| Interacting proteins: P04150 and Q04206 |
Pubmed |
SVM Score :0.0 |
| Negative cross talk between RelA and the glucocorticoid receptor : a possible mechanism for the antiinflammatory action of glucocorticoids . ^^^ In this report we present evidence that the ligand activated glucocorticoid receptor ( GR ) is able to repress RelA mediated activation of the ICAM 1 NF kappa B site . ^^^ |
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| Interacting proteins: P04150 and Q04206 |
Pubmed |
SVM Score :0.0 |
| Distinct domains of the RelA NF kappaB subunit are required for negative cross talk and direct interaction with the glucocorticoid receptor . ^^^ The RelA subunit of NF kappaB and the glucocorticoid receptor mutually repress each others transcriptional activity , thus providing a mechanism for immunosuppression . ^^^ Here , we show by deletions and point mutations that both the Rel homology domain and the transactivation domains of RelA are required for repression of the transcriptional activity of the glucocorticoid receptor in intact cells . ^^^ However , only the Rel homology domain of RelA was found to associate with the glucocorticoid receptor in vitro . ^^^ RelA mutants , not able to repress glucocorticoid receptor activity , but still able to dimerize , behaved as transdominant inhibitors of the repressive activity of wild type RelA . ^^^ |
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| Interacting proteins: P04150 and Q04206 |
Pubmed |
SVM Score :0.0 |
| Negative transcriptional regulation or cross coupling between NF kappa B ( RelA ) and the glucocorticoid receptor ( GR ) is proposed to play a regulatory role in human physiology and disease . ^^^ |
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| Interacting proteins: P04150 and Q04206 |
Pubmed |
SVM Score :0.0 |
| Utilizing green fluorescent protein tagged glucocorticoid receptor ( GR ) and relA as our working model and with judicious use of LMB , we show in living cells that all the soluble components of the nuclear import machinery exit nucleus via exportin1 / CRM1 independent pathway ( s ) . . ^^^ |
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| Interacting proteins: P04150 and Q04206 |
Pubmed |
SVM Score :0.0 |
| As predicted from these results the mineralocorticoid receptor that contains a C terminal zinc finger identical to that of the GR was also able to repress RelA dependent transcription . ^^^ Mutation of a conserved arginine or a lysine in the second zinc finger of the GR DBD ( Arg 488 or Lys 490 in the rat GR ) abolished the ability of GR to inhibit RelA activity . ^^^ In contrast , C terminal zinc finger GR mutants with mutations in the dimerization box or mutations necessary for full transcriptional GR activity were still able to repress RelA dependent transcription . ^^^ In addition , we found that the steroid analog ZK 98299 known to induce GR transrepression of AP 1 had no inhibitory effect on RelA activity . ^^^ |
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| Interacting proteins: P04150 and Q04206 |
Pubmed |
SVM Score :0.0 |
| We demonstrate that RIP 140 antagonized all GR mediated responses tested , which included activation through classical GRE , the synergistic effects of glucocorticoids on AP 1 and Pbx1 / HOXB1 responsive elements , as well as gene repression through a negative GRE and cross talk with NF kappaB ( RelA ) . ^^^ |
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| Interacting proteins: P04150 and Q04206 |
Pubmed |
SVM Score :0.0 |
| That is , we show that the GR zinc binding region ( ZBR ) , which includes the DNA binding and dimerization functions of the receptor , binds directly to the dimerization domain of the RelA subunit of NFkappaB in vitro and that the ZBR is sufficient to associate with RelA bound at NFkappaB response elements in vivo . ^^^ |
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| Interacting proteins: P04150 and Q04206 |
Pubmed |
SVM Score :0.0 |
| The glucocorticoid receptor was shown to repress activity of both the RelA homodimer and the NF kappa B1 / RelA heterodimer . ^^^ |
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| Interacting proteins: P04150 and Q04206 |
Pubmed |
SVM Score :0.0 |
| The p 65 ( RelA ) component of nuclear factor kappaB ( NF kappaB ) and the glucocorticoid receptor ( GR ) mutually repress each other ' s ability to activate transcription . ^^^ |
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| Interacting proteins: P04150 and Q04206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04150 and Q04206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04150 and Q04206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04150 and Q04206 |
Pubmed |
SVM Score :0.0 |
| NA |
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