| Interacting proteins: Q00839 and P04150 |
Pubmed |
SVM Score :0.77117414 |
| The glucocorticoid receptor is associated with the RNA binding nuclear matrix protein hnRNP U . 0.77117414^^^ |
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| Interacting proteins: Q00839 and P04150 |
Pubmed |
SVM Score :0.0 |
| A functional interaction between the NMTS of rat GR and the RNA binding nuclear matrix protein hnRNP U was revealed in cotransfection experiments in which hnRNP U overexpression was found to interfere with the transactivation activity of GR derivatives that possess nuclear matrix binding capacity . ^^^ |
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| Interacting proteins: Q00839 and P04150 |
Pubmed |
SVM Score :0.0 |
| Previously we identified hnRNP U / SAF A as a factor interfering with GR dependent transcription by repressing glucocorticoid induced activation . ^^^ To gain insight into the mechanisms that govern this interference , we have now investigated the transcription of GR dependent reporter genes in Ltk ( ) cells transiently transfected with a variety of hnRNP U constructs . ^^^ We demonstrate that a hnRNP U construct lacking the GR binding domain acts as a dominant negative factor that now enhances GR driven transcription . ^^^ In addition , hnRNP U repression of glucocorticoid induced transcription was found to be dependent on the amount of cotransfected GR , where a high amount of GR leads to ligand inducible repression of GR dependent reporter gene activity by hnRNP U , whereas low amounts of GR showed nearly no effect . ^^^ The relative concentrations of GR , hnRNP U and DNA binding sites for GR are important for the effect of hnRNP U on transcription , suggesting a model where hnRNP U acts as a storage site for intranuclear GR . . ^^^ |
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