| Interacting proteins: P84022 and P04150 |
Pubmed |
SVM Score :1.1090729 |
| Finally , we show that GR interacts with Smad 3 both in vitro and in vivo . 1.1090729^^^ |
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| Interacting proteins: P84022 and P04150 |
Pubmed |
SVM Score :0.0 |
| We previously reported that liganded glucocorticoid receptor ( GR ) repressed TGF beta induction of human plasminogen activator inhibitor 1 gene transcription by directly targeting the transcriptional activation function of Smad 3 . ^^^ Co immunoprecipitation experiments in Hep3B cells indicated that physical interaction between GR and Smad 3 is necessary but not sufficient for repression . ^^^ Physical interaction required activation of Smad 3 by TGF beta but not dexamethasone binding to GR . ^^^ Glutathione S transferase pull down assays demonstrated that several regions of the LBD could mediate GR Smad 3 physical interaction . ^^^ |
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| Interacting proteins: P84022 and P04150 |
Pubmed |
SVM Score :0.0 |
| Smad 3 associated in vitro with GR , with GABPalpha ( via the MH 2 domain ) , and with GABPbeta , Smad 4 only with GABPalpha . ^^^ |
|
| Interacting proteins: P84022 and P04150 |
Pubmed |
SVM Score :0.0 |
| We have reported that liganded glucocorticoid receptor ( GR ) represses TGFbeta transactivation of PAI 1 in Hep3B human hepatoma cells and that it interacts functionally and physically with the C terminal activation domain of Smad 3 , a mediator of TGFbeta signaling . ^^^ |
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