| Interacting proteins: P53041 and P04150 |
Pubmed |
SVM Score :0.74135154 |
| In this work , we show that PP 5 is recovered with both GR that is nuclear and GR that is cytoplasmic in hormone free cells . 0.74135154^^^ |
|
| Interacting proteins: P53041 and P04150 |
Pubmed |
SVM Score :0.0 |
| Since glucocorticoid receptors ( GR ) exist as large heterocomplexes containing hsp 90 bound to TPR proteins , we hypothesized that PP 5 might be associated with these complexes . ^^^ Consistent with this hypothesis , PP 5 specifically co immunoprecipitated with GR from mouse L cell lysates . ^^^ |
|
| Interacting proteins: P53041 and P04150 |
Pubmed |
SVM Score :0.0 |
| Ser / Thr protein phosphatase type 5 ( PP 5 ) is a negative regulator of glucocorticoid receptor mediated growth arrest . ^^^ The suppression of PP 5 expression ( 1 ) markedly increases the association of GR with its cognate DNA binding sequence , ( 2 ) induces GR transcriptional activity without the addition of hormone , and ( 3 ) increases dexamethasone mediated induction of GR reporter activity to a level that is approximately 10 times greater than the maximal response obtainable in the presence of PP 5 . ^^^ PP 5 has no apparent effect on the binding of hormone to the GR , and dexamethasone mediated growth arrest correlates with an increase in p 53 phosphorylation . ^^^ Comparative studies in p 53 wild type , p 53 defective , and p 53 deficient cell lines indicate that either ( 1 ) p 53 participates in GR mediated induction of p 21 ( WAF1 / Cip1 ) , with the hyperphosphorylation of basal p 53 induced by glucocorticoids sufficient for the propagation of an antiproliferative response when PP 5 expression is inhibited , or ( 2 ) PP 5 acts where p 53 mediated and GR induced signaling networks converge to regulate the transcriptional induction of p 21 ( WAF1 / Cip1 ) . ^^^ Thus , aberrant PP 5 expression may have an additive effect on the development of human cancers by promoting cell proliferation via the inhibition of a GR induced antiproliferative signaling cascade , and facilitating neoplastic transformation via the inhibition of a growth arresting p 53 mediated response that guards against genomic instability . . ^^^ |
|
| Interacting proteins: P53041 and P04150 |
Pubmed |
SVM Score :0.0 |
| Serine / threonine protein phosphatase 5 ( PP 5 ) participates in the regulation of glucocorticoid receptor nucleocytoplasmic shuttling . ^^^ CONCLUSION : These studies suggest that PP 5 participates in the regulation of glucocorticoid receptor nucleocytoplasmic shuttling , and that the GR induced transcriptional activity observed when the expression of PP 5 is suppressed by treatment with ISIS 15534 results from the nuclear accumulation of GR in a form that is capable of binding DNA yet still requires agonist to elicit maximal transcriptional activation . . ^^^ Serine / threonine protein phosphatase type 5 ( PP 5 ) associates with the GR heat shock protein 90 complex , and the suppression of PP 5 expression with ISIS 15534 stimulates the activity of GR responsive reporter plasmids , without affecting the binding of hormone to the GR . ^^^ RESULTS : To further characterize the mechanism by which PP 5 affects GR induced gene expression , we employed immunofluorescence microscopy to track the movement of a GR green fluorescent fusion protein ( GR GFP ) that retained hormone binding , nuclear translocation activity and specific DNA binding activity , but is incapable of transactivation . ^^^ The nuclear accumulation of GR GFP , without the addition of glucocorticoids , was also observed when the expression of PP 5 was suppressed by treatment with ISIS 15534 . ^^^ |
|
| Interacting proteins: P53041 and P04150 |
Pubmed |
SVM Score :0.0 |
| Serine / threonine phosphatase 5 ( PP 5 ) can act as a suppresser of p 53 dependent growth suppression and has been reported to associate with several proteins , including the glucocorticoid receptor / heat shock protein 90 complex . ^^^ To characterize the relationship of PP 5 , glucocorticoid receptor activation and p 53 , here we describe the development of chimeric antisense oligonucleotides that potently inhibit human p 53 expression . ^^^ Together , these findings indicate that the basal expression of p 53 plays a functional role in a glucocorticoid receptor mediated response regulating the expression of p 21 ( Waf1 / Cip1 ) via a mechanism that is suppressed by PP 5 and associated with the phosphorylation of p 53 at Ser 15 . . ^^^ |
|
| Interacting proteins: P53041 and P04150 |
Pubmed |
SVM Score :0.0 |
| In this work , we have investigated the effects of four TPR proteins ( FKBP 52 , FKBP 51 , Cyp 40 , and PP 5 ) on hormone binding function of glucocorticoid receptor ( GR ) endogenously expressed in mammalian L 929 cells . ^^^ This result correlated with loss of GR associated FKBP 51 and replacement with PP 5 . ^^^ These results show that immunosuppressive ligands can alter GR hormone binding function by changing the TPR protein composition of receptor complexes and that TPR proteins exert a hierarchical effect on this GR function in the following order : FKBP 52 > PP 5 > FKBP51 . . ^^^ |
|
| Interacting proteins: P53041 and P04150 |
Pubmed |
SVM Score :0.0 |
| TTE ability to detect the presence ( > GR 0 ) of ABD AO ATH on TEE was measured in terms of sensitivity ( SN ) , specificity ( SP ) , positive ( PPV ) and negative ( NPV ) predictive accuracy in patients with adequate and suboptimal images compared to TEE . ^^^ |
|
| Interacting proteins: P53041 and P04150 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P53041 and P04150 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P53041 and P04150 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P53041 and P04150 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P53041 and P04150 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P53041 and P04150 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P53041 and P04150 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P53041 and P04150 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P53041 and P04150 |
Pubmed |
SVM Score :0.0 |
| NA |
|