| Interacting proteins: P48552 and P04150 |
Pubmed |
SVM Score :0.0 |
| Receptor interacting protein 140 binds c Jun and inhibits estradiol induced activator protein 1 activity by reversing glucocorticoid receptor interacting protein 1 effect . ^^^ |
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| Interacting proteins: P48552 and P04150 |
Pubmed |
SVM Score :0.0 |
| Receptor interacting protein 140 ( RIP 140 ) is a corepressor that negatively regulates the ligand induced activity of several nuclear receptors , including the glucocorticoid receptor ( GR ) . ^^^ |
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| Interacting proteins: P48552 and P04150 |
Pubmed |
SVM Score :0.0 |
| However , other target proteins ( e . g . , RIP 140 , and SRC 1 ) , which interact with the GR C terminus , did not show significant interactions with the tau 1 core under our conditions . . ^^^ |
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| Interacting proteins: P48552 and P04150 |
Pubmed |
SVM Score :0.0 |
| In this report we have studied the effect of RIP 140 on gene regulation by the glucocorticoid receptor ( GR ) . ^^^ We demonstrate that RIP 140 antagonized all GR mediated responses tested , which included activation through classical GRE , the synergistic effects of glucocorticoids on AP 1 and Pbx1 / HOXB1 responsive elements , as well as gene repression through a negative GRE and cross talk with NF kappaB ( RelA ) . ^^^ Instead , this suggests that RIP 140 acts as a direct inhibitor of GR function . ^^^ In line with a direct effect of RIP 140 on the GR , we demonstrate a GR RIP 140 interaction in vitro by a glutathione S transferase pull down assay . ^^^ Furthermore , the repressive effect of RIP 140 could partially be overcome by overexpression of the coactivator TIF 2 , which involved a competition between TIF 2 and RIP 140 for binding to the GR . . ^^^ |
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| Interacting proteins: P48552 and P04150 |
Pubmed |
SVM Score :0.0 |
| Furthermore , the ER LBD and the TR compete in vitro for the related p 160 coactivators , SRC1a and GRIP 1 ( glucocorticoid receptor interacting protein 1 ) , or the putative corepressor , RIP 140 . ^^^ |
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| Interacting proteins: P48552 and P04150 |
Pubmed |
SVM Score :0.0 |
| Coexpression of other AF 2 interacting proteins , including the p 160 factors , steroid receptor coactivator 1a ( SRC 1a ) and glucocorticoid receptor interacting protein 1 ( GRIP 1 ) , had negligible effects on ER alpha / Pit 1 cooperative activation , but partially relieved RIP 140 inhibition . ^^^ |
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| Interacting proteins: P48552 and P04150 |
Pubmed |
SVM Score :0.0 |
| We have investigated the mechanism of transcriptional activation by the GR by studying the role of the receptor interacting protein RIP 140 . ^^^ Both in vivo and in vitro protein protein interaction assays revealed a ligand dependent interaction between the GR and RIP 140 . ^^^ The ligand binding domain of the GR was sufficient for this interaction , while both the N and C terminal regions of RIP 140 bound to the receptor . ^^^ In a yeast transactivation assay RIP 140 and SRC 1 , a member of the steroid receptor coactivator family of proteins , both enhanced the transactivation activity of a GR protein ( GRA 1 ) in which the potent N terminal tau 1 transactivation domain has been deleted . ^^^ Thus , in yeast cells a stimulation of receptor activity was observed , while in mammalian cells RIP 140 repressed GR function . ^^^ |
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| Interacting proteins: P48552 and P04150 |
Pubmed |
SVM Score :0.0 |
| Regulation of glucocorticoid receptor activity by 14 3 3 dependent intracellular relocalization of the corepressor RIP 140 . ^^^ In transfection assays , RIP 140 antagonizes 14 3 3 enhanced GR transactivation . ^^^ In summary , our findings suggest that 14 3 3 mediated intracellular relocalization of the GR corepressor RIP 140 might be a novel mechanism to enhance glucocorticoid responsiveness of target genes . ^^^ |
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| Interacting proteins: P48552 and P04150 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P48552 and P04150 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P48552 and P04150 |
Pubmed |
SVM Score :0.0 |
| NA |
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