| Interacting proteins: P42229 and P04150 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42229 and P04150 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42229 and P04150 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42229 and P04150 |
Pubmed |
SVM Score :0.68413985 |
| Expression level dependent contribution of glucocorticoid receptor domains for functional interaction with STAT 5 . 0.68413985^^^ |
|
| Interacting proteins: P42229 and P04150 |
Pubmed |
SVM Score :0.51554375 |
| Although these pathways appear to be unrelated , we show here that the glucocorticoid receptor can act as a transcriptional co activator for Stat 5 and enhance Stat 5 dependent transcription . 0.51554375^^^ |
|
| Interacting proteins: P42229 and P04150 |
Pubmed |
SVM Score :0.53329486 |
| Our results suggest that in lactogenic hormone response elements glucocorticoid receptor molecules bound to nonclassical half palindromic sites gain competence as transcriptional activators by the interaction with Stat 5 molecules binding to vicinal sites . . 0.53329486^^^ |
|
| Interacting proteins: P42229 and P04150 |
Pubmed |
SVM Score :0.54031004 |
| We find that glucocorticoid receptor , mineralocorticoid receptor and progesterone receptor synergize with Stat 5 in the induction of the transcription from the beta casein gene promoter . 0.54031004^^^ |
|
| Interacting proteins: P42229 and P04150 |
Pubmed |
SVM Score :0.5957559 |
| Previously , physical association between GR and Stat 5 has been shown to enhance Stat 5 dependent and suppress GR dependent transcription . 0.5957559^^^ |
|
| Interacting proteins: P42229 and P04150 |
Pubmed |
SVM Score :0.66781869 |
| Thus , synergistic and antagonistic interactions between GR , NF kappa B , and STAT 5 involve ( a ) cross talk mechanisms influencing the activation of STAT 5 and ( b ) promoter dependent interactions modulating the DNA binding activity of the transcription factors . . 0.66781869^^^ |
|
| Interacting proteins: P42229 and P04150 |
Pubmed |
SVM Score :0.85659433 |
| This is in good agreement with the demonstration that the DNA binding defective GR is still able to interact with phosphorylated Stat 5 proteins , suggesting that transcriptional regulation by protein protein interaction forms the basis of glucocorticoid action in this process . 0.85659433^^^ |
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| Interacting proteins: P42229 and P04150 |
Pubmed |
SVM Score :0.87969875 |
| We show that Stat 5 proteins physically interact with GR and GR is present in vivo on Stat 5 dependent IGF 1 and ALS regulatory regions . 0.87969875^^^ |
|
| Interacting proteins: P42229 and P04150 |
Pubmed |
SVM Score :0.0 |
| This effect was mediated through the glucocorticoid receptor and independent of STAT 5 activity . ^^^ |
|
| Interacting proteins: P42229 and P04150 |
Pubmed |
SVM Score :0.0 |
| Specific DNA binding of Stat 5 , but not of glucocorticoid receptor , is required for their functional cooperation in the regulation of gene transcription . ^^^ It has been demonstrated that Stat 5 and the glucocorticoid receptor form a molecular complex which cooperates in the induction of transcription of the beta casein gene . ^^^ We have defined the DNA sequence requirements for this cooperative mechanism and have delimited the functional domains in Stat 5 and the glucocorticoid receptor that are necessary for the functional interaction . ^^^ We find that the Stat 5 response element ( Stat5RE ) within the beta casein gene promoter is sufficient to elicit the cooperative action of Stat 5 and the glucocorticoid receptor on transcription . ^^^ Deletion of the transactivation domain of Stat 5 results in a molecule which can not mediate transactivation by itself but can still cooperate with the glucocorticoid receptor . ^^^ |
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| Interacting proteins: P42229 and P04150 |
Pubmed |
SVM Score :0.0 |
| Mechanism of interaction between the glucocorticoid receptor and Stat 5 : role of DNA binding . ^^^ The functional interaction between the glucocorticoid receptor ( GR ) and the signal transducer and activator of transcription 5 ( Stat 5 ) was investigated by studying the synergistic activation of beta cascin gene transcription by prolactin and glucocorticoids . ^^^ The synergism was shown to be mediated by a complex hormone response region with multiple binding sites for Stat 5 , the glucocorticoid receptor , and CCAAT / enhancer binding proteins ( C / EBP ) . ^^^ HC 11 mammary epithelial cells , which contain physiological levels of GR and Stat 5 , and COS 7 cells overexpressing GR and Stat 5 were employed . ^^^ In both cell types intact binding sites for Stat 5 and the GR were a prerequisite for the synergism , whereas C / EBP sites were only required in HC 11 cells . ^^^ |
|
| Interacting proteins: P42229 and P04150 |
Pubmed |
SVM Score :0.0 |
| In the whey protein gene promoters , these include binding sites for nuclear factor ( NF ) 1 , as well as the glucocorticoid receptor ( GR ) and signal transducers and activators of transcription ( Stat 5 ) . ^^^ In the casein promoters , these include binding sites for Stat 5 , Yin Yang 1 ( YY 1 ) , GR and the CCAAT / enhancer binding protein ( C / EBP ) . ^^^ |
|
| Interacting proteins: P42229 and P04150 |
Pubmed |
SVM Score :0.0 |
| Immunodepletion experiments indicated that a fraction of GR and Stat 5 proteins are not associated , suggesting that there are different intracellular pools of these proteins . ^^^ Although the GR does not bind the Stat 5 DNA binding site directly , it could be detected with the Stat 5 DNA complex . ^^^ These results suggest that glucocorticoids affect milk protein gene expression via association of the GR with Stat 5 . ^^^ |
|
| Interacting proteins: P42229 and P04150 |
Pubmed |
SVM Score :0.0 |
| We recently showed cross talk between Stat 5 and the glucocorticoid receptor . ^^^ The activated glucocorticoid receptor forms a complex with Stat 5 and enhances Stat 5 mediated transcriptional induction . ^^^ Here , we investigated the role of p300 / CBP ( CREB binding protein ) , a transcriptional coactivator of several groups of transcription factors , in Stat 5 mediated transactivation and in the cross talk between Stat 5 and the glucocorticoid receptor . p300 / CBP acts as a coactivator of Stat 5 . ^^^ Coimmunoprecipitation and mammalian two hybrid assays demonstrate a direct interaction between the carboxyl terminal transactivation domain of Stat 5 and p300 / CBP . p300 / CBP also positively interacts with the glucocorticoid receptor and enhances glucocorticoid receptor dependent transcriptional activation of the mouse mammary tumor virus long terminal repeat promoter . ^^^ Overexpression of p300 / CBP does not counteract the Stat 5 mediated inhibition of glucocorticoid receptor dependent transactivation , i . e . the repression of the glucocorticoid response by Stat 5 is not a consequence of competition for limiting amounts of p300 / CBP . . ^^^ |
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| Interacting proteins: P42229 and P04150 |
Pubmed |
SVM Score :0.0 |
| Glucocorticoid receptor / signal transducer and activator of transcription 5 ( STAT 5 ) interactions enhance STAT 5 activation by prolonging STAT 5 DNA binding and tyrosine phosphorylation . ^^^ Previous studies have demonstrated that the downstream effectors of these pathways , signal transducer and activator of transcription 5 ( STAT 5 ) and the glucocorticoid receptor ( GR ) , are associated via protein protein interactions and act synergistically to enhance beta casein gene transcription . ^^^ Indirect immunofluorescence microscopy was used to demonstrate that PRL activated STAT 5 can translocate GR into the nucleus , and that ligand bound GR can translocate STAT 5 into the nucleus . ^^^ To better understand the mechanism of transcriptional synergy between STAT 5 and GR , experiments were performed in cells transiently transfected with STAT 5 alone or with STAT 5 and GR . ^^^ GR cotransfection enhanced the DNA binding activity of STAT 5 without affecting STAT 5 protein levels . ^^^ |
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| Interacting proteins: P42229 and P04150 |
Pubmed |
SVM Score :0.0 |
| Yin yang 1 and glucocorticoid receptor participate in the Stat 5 mediated growth hormone response of the serine protease inhibitor 2 . 1 gene . ^^^ The glucocorticoid receptor was also present in GHINF , and Stat 5 co immunoprecipitates with glucocorticoid receptor in hepatic nuclear extracts from rats treated with GH . ^^^ |
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| Interacting proteins: P42229 and P04150 |
Pubmed |
SVM Score :0.0 |
| Positive enhancement of STAT 5 trans activation potential is provided by the glucocorticoid receptor . ^^^ An additional regulatory loop is provided by the reactivation of the short form of STAT 5 through glucocorticoid receptor association . ^^^ |
|
| Interacting proteins: P42229 and P04150 |
Pubmed |
SVM Score :0.0 |
| Cooperative effects of STAT 5 ( signal transducer and activator of transcription 5 ) and C / EBPbeta ( CCAAT / enhancer binding protein beta ) on beta casein gene transcription are mediated by the glucocorticoid receptor . ^^^ This CoRE contains binding sites for STAT 5 ( signal transducer and activator of transcription 5 ) and C / EBPbeta ( CCAAT / enhancer binding protein beta ) and several half sites for glucocorticoid receptor ( GR ) . ^^^ Cooperative transactivation was observed when all three factors were expressed , but unexpectedly was not seen between STAT 5 and C / EBPbeta in the absence of full length , transcriptionally active GR . ^^^ Cooperativity required the amino terminal transactivation domain of C / EBPbeta , and neither C / EBPalpha nor C / EBPdelta was able to substitute for C / EBPbeta when cotransfected with STAT 5 and GR . ^^^ Different GR determinants were needed for transcriptional cooperation between STAT 5 and GR as compared with those required for all three transcription factors . ^^^ |
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| Interacting proteins: P42229 and P04150 |
Pubmed |
SVM Score :0.0 |
| Differential interactions of specific nuclear factor 1 isoforms with the glucocorticoid receptor and STAT 5 in the cooperative regulation of WAP gene transcription . ^^^ Point mutations in the binding sites for specific transcription factors present within this CoRE have demonstrated the importance of both nuclear factor 1 ( NFI ) and STAT 5 as well as cooperative interactions with the glucocorticoid receptor ( GR ) in the regulation of WAP gene expression in the mammary gland of transgenic mice . ^^^ Some but not all of these NFI isoforms synergistically activate WAP gene transcription in cooperation with GR and STAT 5 , as determined using transient cotransfection assays in JEG 3 cells . ^^^ |
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| Interacting proteins: P42229 and P04150 |
Pubmed |
SVM Score :0.0 |
| Positive enhancement of Stat 5 transactivation potential is provided by the glucocorticoid receptor ( GR ) . ^^^ An additional regulatory loop is provided by the reactivation of the short form of Stat 5 through GR association . . ^^^ |
|
| Interacting proteins: P42229 and P04150 |
Pubmed |
SVM Score :0.0 |
| Immunoprecipitation experiments revealed that phosphorylated STAT 5 and GCR formed immune complexes . ^^^ This association may lead to retardation of GCR nuclear translocation because IL 2 was not able to induce steroid insensitivity in splenocytes from STAT 5 knockout mice . ^^^ |
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| Interacting proteins: P42229 and P04150 |
Pubmed |
SVM Score :0.0 |
| These findings support a model in which STAT 5 , in concert with the glucocorticoid receptor , recruits a multifunctional coactivator complex to initiate the PRL dependent transcription . . ^^^ |
|
| Interacting proteins: P42229 and P04150 |
Pubmed |
SVM Score :0.0 |
| Signal transducer and activator of transcription 5 ( STAT 5 ) is a transcription factor involved in the proliferation , differentiation and survival of haematopoietic cells , which interacts with glucocorticoid receptor . ^^^ This suggests that dexamethasone could act on proliferation , differentiation and apoptosis during eosinophil differentiation through an association of STAT 5 with the glucocorticoid receptor . . ^^^ |
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| Interacting proteins: P42229 and P04150 |
Pubmed |
SVM Score :0.0 |
| Erythroid progenitor renewal versus differentiation : genetic evidence for cell autonomous , essential functions of EpoR , Stat 5 and the GR . ^^^ Using primary erythroblast cultures from these mutants , we present genetic evidence that functional GR , EpoR , and Stat 5 are essential for erythroblast renewal in vitro . ^^^ |
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| Interacting proteins: P42229 and P04150 |
Pubmed |
SVM Score :0.0 |
| Here we show that two proline juxtaposed serine residues within the transactivation domain of Stat5a are phosphorylated in the mammary gland during late gestation and lactation , and that these phosphorylation sites inhibit the transcriptional activity of Stat5a in the absence of glucocorticoid receptor costimulation . ^^^ |
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| Interacting proteins: P42229 and P04150 |
Pubmed |
SVM Score :0.0 |
| Transfection experiments show enhancement of human type 2 3beta HSD promoter activity by coexpression of the glucocorticoid receptor ( GR ) and Stat5A and treatment with 100nM dexamethasone . ^^^ |
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| Interacting proteins: P42229 and P04150 |
Pubmed |
SVM Score :0.0 |
| STAT5A promotes adipogenesis in nonprecursor cells and associates with the glucocorticoid receptor during adipocyte differentiation . ^^^ Moreover , we have shown that STAT5A is associated with the glucocorticoid receptor during adipogenesis in a highly regulated manner . . ^^^ |
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| Interacting proteins: P42229 and P04150 |
Pubmed |
SVM Score :0.0 |
| Electrophoretic Mobility Shift Assays ( EMSA ) show that the region ( bases pairs 386 to 322 , where there is a STAT binding site , TTTCAAAAA ) binds to STAT5a , not to glucocorticoid receptor ( GR ) or other STAT factors . ^^^ |
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