Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P04141 and P15509	Pubmed	SVM Score :1.1111931
GM CSF binds to specific cellular receptors ( GM CSFR ) which belong to a recently described supergene family . 1.1111931^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.66080149
High affinity GMR interacts with GM CSF in a species specific manner , therefore one can analyze the effects of hGMR signals on differentiation of mouse hemopoietic progenitors using hGM CSF . 0.66080149^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.73184538
The effects of granulocyte / macrophage colony stimulating factor ( GM CSF ) are mediated by interaction with its composite receptor ( GMR ) , which consists of a unique alpha subunit ( GMR alpha ) and a beta subunit ( GMR beta ) that is common to the receptors for GM CSF , interleukin 3 , and interleukin 5 . 0.73184538^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.94074055
Thus , GM CSF binds to its receptor CD 116 , and consequently activates Syk phosphorylation , which leads to induce CXCR 3 expression . gamma IP 10 and Mig can induce Syk , Cbl , and Cbl b phosphorylation in CD 34 ( + ) progenitors by means of CXCR 3 . gamma IP 10 or Mig has induced neither chemotaxis nor adhesion in GM CSF stimulated Cbl b blocked CD 34 ( + ) hemopoietic progenitors , whereas SDF 1alpha induces both chemotaxis and adhesion in these cells . 0.94074055^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.61821476
This technology has been applied to the study of interactions between human granulocyte macrophage colony stimulating factor ( GM CSF ) and its receptor ( GM CSFR ) . 0.61821476^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
We examined the changes in mRNA expression of granulocyte macrophage colony stimulating factor receptor beta subunit ( GM CSFR beta subunit ) , which was a common subunit of a high affinity interleukin 3 receptor ( IL 3R ) and a high affinity GM CSFR , and interleukin 6 receptor ( IL 6R ) during megakaryocyte development in a human megakaryocytic leukemia cell line ( CMK ) which could proliferate and / or differentiate in the presence of 12 O tetradecanoylphorbol 13 acetate ( TPA ) , IL 3 , GM CSF , and IL 6 . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
The gene encoding the granulocyte macrophage colony stimulating factor receptor alpha subunit ( CSF2RA ) has previously been mapped to the pseudoautosomal region of the human sex chromosomes . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Granulocyte macrophage colony stimulating factor ( GM CSF ) activates a broad range of myeloid cells through binding to high affinity receptors ( GM CSF R ) consisting of at least two distinct subunits , GM CSF R alpha and GM CSF R beta . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
The granulocyte macrophage colony stimulating factor receptor alpha chain gene ( GM CSF R ) has recently been localised to the pseudoautosomal region of the sex chromosomes . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
The granulocyte / macrophage colony stimulating factor ( GM CSF ) receptor ( GMR ) transduces a signal that results in the proliferation , differentiation , and functional activation of hematopoietic cells . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Affinity purification of human granulocyte macrophage colony stimulating factor receptor alpha chain . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Chimaeric IL 2 receptor beta and gamma chains constructed with the heterodimeric extracellular regions of the granulocyte macrophage colony stimulating factor receptor ( GM CSFR ) also provided the IL 2R signal . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Flow cytometric analysis of five myeloma cell lines ( RPMI 8226 , ARH 77 , KMM 1 , U 266 , and Hs ) and myeloma cells freshly isolated from eight patients showed that interleukin 1 receptor ( IL 1R ) type 1 and type 2 , IL 2R alpha and beta chains , IL 4R , IL 6R , IL 7R , IL 8R , granulocyte macrophage colony stimulating factor receptor ( GM CSFR ) , c kit ( stem cell factor receptor [ SCFR ] ) , membrane bound stem cell factor ( MBSCF ) , and tumor necrosis factor ( TNF ) receptors type 1 and type 2 were not always detected on the myeloma cells . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Granulocyte macrophage colony stimulating factor receptor ( GMR ) is composed of two distinct subunits alpha and beta , and the cytoplasmic domains of both subunits are essential to transduce signals . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
The granulocyte / macrophage colony stimulating factor ( GM CSF ) receptor ( GMR ) is a heterodimeric receptor expressed by myeloid lineage cells . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
While the primary targets for granulocyte macrophage colony stimulating factor ( GM CSF ) are hematopoietic precursors and mature myeloid cells , GM CSF receptors ( GMR ) are also found on normal tissues including placenta , endothelium , and oligodendrocytes as well as certain malignant cells . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Whereas the human IL 3R alpha gene is tightly linked with the granulocyte macrophage colony stimulating factor receptor alpha subunit ( GM CSFR alpha ) gene in the pseudoautosomal region of the 10 and Y chromosomes , the mIL 3R alpha gene ( II3ra ) is located in the proximal region of mouse chromosome 14 , separated from the mouse GM CSFR alpha gene , which is on chromosome 19 . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Demonstration of the low affinity alpha subunit of the granulocyte macrophage colony stimulating factor receptor ( GM CSF R alpha ) on human trophoblast and uterine cells . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Molecular cloning of a soluble form of the granulocyte macrophage colony stimulating factor receptor alpha chain from a myelomonocytic cell line . ^^^ To analyze the molecular and functional characteristics of a soluble form of the granulocyte macrophage colony stimulating factor receptor alpha chain ( sGM CSFR alpha ) , and analyze transcript expression in immune cells and the cellular constituents of rheumatoid arthritis synovial tissue . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
To determine the expression and function of the granulocyte macrophage colony stimulating factor ( GM CSF ) receptor alpha chain ( GMR alpha ) during hematopoiesis and on leukemic cells , monoclonal antibodies were raised by immunizing mice with cells expressing high levels of human GMR alpha . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Interleukin ( IL ) 3 , granulocyte macrophage colony stimulating factor , and IL 5 receptors ( IL 3R , GMR , and IL 5R ) are composed of the alpha chain specific to each and the common beta chain , and both the alpha and beta subunits are members of the cytokine receptor superfamily . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Two distinct cDNA clones , corresponding to alternative mRNA splicing variants of the alpha subunit of the granulocyte macrophage colony stimulating factor receptor ( GM CSF R alpha ) were isolated from human blood mononuclear cells by RT PCR . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
The results suggest a model of transactivation cascade for HGFR such as interleukin 6 receptor ( IL 6R ) , IL 3R , GM colony stimulating factor receptor ( GM CSFR ) , and erythropoietin receptor ( EpR ) , whereby each HGF upmodulates the R ( s ) for distal acting HGF ( s ) . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
They included colony stimulating factor receptor ( CSF2R ) , SRY ( TDF ) , ZFY , RPS4Y ( ribosome protein 4S ) , ADMLY ( Kallman syndrome ) , AMGL ( amelogenin , which we cloned ourselves ) and so on . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Granulocyte macrophage colony stimulating factor ( GM CSF ) binds to the high affinity GM CSF receptor ( GMR ) consisting of alpha and beta subunits and induces rapid tyrosine phosphorylation , activation of early response genes , and proliferation of hematopoietic cells . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Neutralizing and nonneutralizing monoclonal antibodies to the human granulocyte macrophage colony stimulating factor receptor alpha chain . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
To identify domains in hematopoietic growth factor receptors that are important for signal transduction , a hybrid receptor ( GMER ) was constructed by splicing the DNA of the entire extracellular and transmembrane domains of the human granulocyte macrophage colony stimulating factor ( GM CSF ) receptor alpha 2 subunit ( GMR ) to the cytoplasmic domain of the murine erythropoietin receptor ( mEpoR ) . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Only little is known about the expression of a functional granulocyte macrophage colony stimulating factor receptor ( GMR ) and its ligands in human colon carcinoma cell lines . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
The high affinity and functional granulocyte macrophage colony stimulating factor receptor ( GMR ) is composed of two distinct subunits , alpha and beta ; and the cytoplasmic domain of the beta subunit is essential to transduce growth promoting signals . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
The intracytoplasmic tail of the granulocyte macrophage colony stimulating factor receptor ( GM CSFR ) beta c chain is essential for the activation of ligand mediated signal transduction pathways in myeloid cells . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
The granulocyte macrophage colony stimulating factor receptor ( GM CSFR ) is composed of at least two chains ( alpha and beta ) . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
To determine whether heterodimeric PRLr complexes were capable of ligand induced signaling and cellular proliferation , chimeras consisting of the extracellular domain of either the alpha or beta subunit of human granulocyte macrophage colony stimulating factor receptor ( GM CSFr ) and the intracellular domain of the rat intermediate or short PRLr isoforms ( PRLr 1 or PRLr S ) were synthesized . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Cytokine R detectable on HMC 1 but not MC were granulocyte macrophage colony stimulating factor ( GM CSFR ) alpha ( CD 116 ) , CD 40 , Apo 1 / FAS ( CD 95 ) and gp 130 ( CD 130 ) . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
The high affinity receptor for granulocyte macrophage colony stimulating factor ( GMR ) comprises at least 2 distinct subunits , alpha and beta common ( beta c ) , whereas the normal erythropoietin receptor ( nEpoR ) comprises only one known subunit . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Granulocyte macrophage colony stimulating factor ( GM CSF ) receptors ( GMR ) are expressed on myeloid cells throughout their maturational sequence . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
A flow cytometric method to quantify the Granulocyte macrophage colony stimulating factor receptor ( GM CSFr ) on human cells is described . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
A dominant negative granulocyte macrophage colony stimulating factor receptor alpha chain reveals the multimeric structure of the receptor complex . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
We recently demonstrated that a chimeric receptor ( GMER ) that is composed of the extracellular and transmembrane domains of the human granulocyte macrophage colony stimulating factor ( GM CSF ) receptor alpha chain ( GMR alpha ) and the cytoplasmic domain of the murine erythropoietin receptor mEpoR binds hGM CSF with low affinity ( 3 nM ) and confers both proliferative and differentiation signals to stably transfected murine Ba / F3 cells . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
The receptor for granulocyte / macrophage colony stimulating factor ( GM CSF ) consists of a ligand specific low affinity binding chain ( GM CSFR alpha ) and a second chain that is required for high affinity binding and signal transduction . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
In contrast , introduction and activation of the interleukin 5 receptor ( IL 5R ) or of the granulocyte macrophage colony stimulating factor receptor ( GM CSFR ) results in proliferation only . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
The granulocyte macrophage colony stimulating factor receptor ( GM CSFR ) is a potential target for toxin directed therapy , because it is overexpressed on many leukemias and solid tumors and apparently not on stem cells . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
All DDC were highly positive for granulocyte macrophage colony stimulating factor receptor ( GM CSFR ) alpha chain ( mAb hGM CSFR M 1 ; SC 06 ; SC 04 , and 8G6 ) and to a lesser extent for the common beta chain of GM CSFR , IL 3R and IL 5R ( mAb 3D7 ) . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
To investigate the molecular basis of this , we generated a clone of transfected NIH3T3 cells that constitutively expressed the human granulocyte macrophage colony stimulating factor receptor ( GM CSF R ) beta chain and inducibly expressed the human GM CSF R alpha chain . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
The human granulocyte macrophage colony stimulating factor receptor ( GM CSFR ) alpha chain RNA is alternatively spliced to yield receptor isoforms . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
The granulocyte macrophage colony stimulating factor ( GM CSF ) receptor ( GMR ) is a heterodimeric receptor expressed by myeloid lineage cells . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
FDCs in the follicular apical light zone expressed transforming growth factor beta receptor 2 ( TGF betaR 2 ) , granulocyte macrophage colony stimulating factor receptor alpha ( GM CSFRalpha ; CDw 116 ) , tumor necrosis factor receptor 1 ( TNFR 1 ; CD120a ) , interleukin 1 receptor 2 ( IL 1R 2 ; CDw121b ) , IL 2 receptor beta ( IL 2Rbeta ; CD 122 ) , IL 4 receptor ( IL 4R ; CDw 124 ) , and IL 6 receptor ( IL 6R ; CD 126 ) , among the 10 receptors examined . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Self peptides derived from the carboxy terminal end of the breakpoint cluster region ( bcr ) protein , as well as several differentiation stage and tissue specific self antigens characteristic of early stages of myeloid differentiation , such as c fes , c pim , granulocyte macrophage colony stimulating factor receptor alpha chain , proteinase 3 , and cathepsin G , were identified . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
To identify and test the functional stoichiometry of those PRLr motifs required for transduction and growth , chimeras consisting of the extracellular domain of either the alpha or beta subunit of human granulocyte macrophage colony stimulating factor ( GM CSF ) receptor ( GM CSFr ) and the intracellular domain of the rat PRLr were synthesized . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
HRMCs were also negative for CD 116 ( granulocyte macrophage colony stimulating factor [ GM CSF ] receptor alpha ) , CD 123 ( interleukin [ IL ] 3Ralpha ) , CD121a ( IL 1R type 1 ) , CD 122 ( IL 2Rbeta ) , and CD 127 ( IL 7R ) and were also found to lack C5aR ( CD 88 ) . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Granulocyte macrophage colony stimulating factor ( GM CSF ) activity is mediated by a cellular receptor ( GM CSFR ) that is comprised of an alpha chain ( GM CSFRalpha ) , which specifically binds GM CSF , and a beta chain ( betac ) , shared with the interleukin 3 and interleukin 5 receptors . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
The high affinity human granulocyte macrophage colony stimulating factor ( GM CSF ) receptor ( GMR ) consists of an alpha ( GMRalpha ) and a common beta ( betac ) subunit . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
The inhibition of DC differentiation by RCC CM was preceeded by an induction of M CSF receptor ( M CSFR ; CD 115 ) and a loss of granulocyte macrophage colony stimulating factor receptor ( GM CSFR ; CD 116 ) expression at the surface of CD 34 ( + ) cells , two phenomenon reversed by anti IL 6 / IL 6R and anti M CSF antibodies , respectively . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Cells from all samples strongly expressed c kit , granulocyte colony stimulating factor receptor ( G CSFR ) , c fms ( macrophage colony stimulating factor receptor ) , interleukin 3 receptor alpha chain ( IL 3Ralpha ) , and granulocyte macrophage colony stimulating factor receptor alpha chain ( GM CSFRalpha ) , and these data corresponded well to the growth responsiveness to the cytokines . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
To analyze the function of each subunit of the receptor for granulocyte macrophage colony stimulating factor ( GM CSF ) , GMR , we previously generated a single chain chimeric receptor by fusion of the extracellular and transmembrane domain from the alpha subunit ( alpha GMR ) to the intracellular part of the beta subunit ( beta GMR ) introducing an additional glutamate residue at the fusion site ( alpha / beta GMR ) . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
The granulocyte macrophage colony stimulating factor ( GM CSF ) receptor ( GMR ) is composed of two chains that belong to the superfamily of cytokine receptors typified by the growth hormone receptor . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Granulocyte macrophage colony stimulating factor ( GM CSF ) is an important hematopoietic cytokine that exerts its effects by interaction with the GM CSF receptor ( GMR ) on the surface of responsive cells . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Furthermore , chimeric receptors consisting of cytosolic and transmembrane part of ovine GHR or ovine PRLR and ECDs of human granulocyte macrophage colony stimulating factor receptor ( GM CSFR ) alpha or beta were constructed . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Both KA and MT 1 + 2 deficiency significantly affected the expression of MT 3 , granulocyte macrophage colony stimulating factor ( GM CSF ) and its receptor ( GM CSFr ) . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
CD 116 ( granulocyte macrophage colony stimulating factor receptor ) . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Among the downregulated genes in DCs were genes that encode proteins of cell cycle regulation : mitotic growth and transcription activator , platelet derived growth factor receptor beta subunit , interleukin 2 receptor ( IL 2R ) gamma subunit , IL 7R alpha subunit , leucocyte interferon gamma ( IFN gamma ) and granulocyte macrophage colony stimulating factor receptor ( GM CSFR ) . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
The receptors for human granuloctye macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) , and IL 5 are composed of a ligand specific alpha chain ( eg , alpha GM CSF receptor [ alpha GMR ] ) and a common beta subunit ( beta GMR ) . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate the iufluence of dexamethasone ( DM ) on expression of interleukin 5 receptor alpha ( IL 5R alpha ) , interleukin 3 receptor alpha ( IL 3R alpha ) , granulocyte macrophage colony stimulating factor receptor alpha ( GM CSFR alpha ) and common beta receptor ( beta cR ) in bronchalveolar lavage fluid ( BALF ) eosinophils in guinea pig asthma model , and to study the mechanism of promotion effect of steroid on eosinophil apoptosis . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Cultured fibroblasts obtained from skin biopsies in SSc involved and non involved areas and norrmal skin fibroblasts were cultured with different doses of granulocyte macrophage colony stimulating factor ( GM CSF ) to study the effects of this factor on the expression of GM CSF receptor ( GM CSFR ) on fibroblast proliferation and cellular adhesion structures . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Zidovudine ( AZT ) treatment suppresses granulocyte monocyte colony stimulating factor receptor type alpha ( GM CSFR alpha ) gene expression in murine bone marrow cells . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
The Granulocyte Macrophage Colony Stimulating Factor ( GM CSF ) is a hematopoietic growth factor that regulates the in vitro and in vivo proliferation and differentiation of hematopoietic cells through the interaction with a specific heterodimeric receptor complex ( GM CSFR ) , consisting of an alpha and a beta chain with molecular weights of 80 and 120 KDa , respectively . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
We have now extended this concept by using extracellular domains of the heteromeric granulocyte / macrophage colony stimulating factor receptor ( GM CSFR ) . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
CD 116 [ granulocyte macrophage colony stimulating factor receptor alpha ( GM CSFRalpha ) ] and CD 123 ( IL 3Ralpha ) were expressed on MC precursors on day 14 , but disappeared thereafter . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
The cytokine granulocyte macrophage colony stimulating factor ( GM CSF ) regulates hematopoiesis and the function of mature host defense cells through the GM CSF receptor ( GMR ) , which is composed of alpha ( alphaGMR ) and beta ( betaGMR ) subunits . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Binding studies confirmed the presence of receptors for GM CSF ( GM CSFR ) on EC . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
For the samples in groups A and B , RA affected neither G CSF receptor ( GR ) nor GM CSF receptor ( GMR ) . ^^^ This finding supports the hypothesis that the increase of GMR is one of the causes of the stimulative effects of RA on cells cultured with GM CSF in group C . . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
The receptor for human granulocyte macrophage ( GM ) CSF ( GMR ) is a heterodimer , consisting of an alpha chain ( GMR alpha ) and a beta chain ( GMR beta ) . ^^^ While GMR alpha is capable of binding GM CSF , GMR beta is necessary for signal transduction . ^^^ We have recently demonstrated that tyrosine 750 ( Y 750 ) in GMR beta is a site of GM CSF induced phosphorylation and this site may contribute to the maintenance of cellular viability in response to GM CSF . ^^^ To investigate possible contributions made by additional GMR beta cytoplasmic tyrosine residues to receptor function , we mutated other selected tyrosine residues to phenylalanine and tested for any defects in signaling . in the present study , we show that Y 577 is required for phosphorylation of Shc and an Shc associated p 140 in response to GM CSF . ^^^ Interestingly , mutation of Y 750 reduced detectable GM CSF induced tyrosine phosphorylation of GMR beta , suggesting that the reduction of Shc phosphorylation associated with that mutant might be actually due to a failure to phosphorylate Y 577 . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
High affinity receptor for IL 5 ( IL 5R ) , a predominant eosinophil maturation factor , is composed of an IL 5 binding alpha chain ( IL 5R alpha ) and a signal transducing beta chain that is shared by IL 3 and granulocyte macrophage CSF ( GM CSF ) receptors ( IL 3R and GM CSFR ) . ^^^ By Northern blot analysis of mRNAs obtained from normal human blood eosinophils , we show in this report that the hematopoietic cytokines IL 5 , IL 3 , and GM CSF down regulate IL 5R alpha mRNA while up regulating alpha chain mRNAs for both IL 3R and GM CSFR as well as the beta chain mRNA . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Receptors for IL 1beta , IL 6 , and GM CSF were readily detectable in all epithelial cell preparations at levels equal to ( GM CSFR ) or lower than those seen on monocytes . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
As a further comparison of the actions of the two CSFs , CSF 1R and GM CSFR levels were measured by flow cytometry , and it was shown that GM CSFR levels decreased within 5 days of culture , independent of the conditions examined . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
In order to understand a possible inter relationship between the G CSF and GM CSF induced leukocyte responses and expression levels of receptors for G CSF ( G CSFr ) and GM CSF ( GM CSFr ) , the levels of each receptor and CSF were measured in patients with basophilia ( 8 ) , eosinophilia ( 14 ) and bacterial infection showing neutrophilia ( 12 ) in comparison with normal healthy adults ( 12 ) and children ( 14 ) . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
We analyzed the comparative amounts of granulocyte colony stimulating factor ( G CSFr ) and granulocyte macrophage CSF ( GM CSFr ) receptors expressed on neutrophils and monocytes in measles patients to investigate the role of these CSFrs in the development of leukopenia including neutropenia and monocytopenia in measles . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Arrangement and localization of the human GM CSF receptor alpha chain gene CSF2RA within the 10 Y pseudoautosomal region . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
We identified a truncated , soluble form of the low affinity GM CSF receptor ( GMR ) in chorio carcinoma cells . ^^^ Expression of the soluble GMR cDNA in murine psi AM cells as well as GM CSF dependent myeloid 32Dc13 cells produced a secreted protein that retained its capacity to bind GM CSF in solution . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
We have therefore studied the coding sequence of the GM CSF receptor alpha chain ( GM CSFR alpha ) in patients with acute myeloid leukaemia ( AML ) and non AML controls using single strand conformation polymorphism ( SSCP ) analysis . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
The membrane proximal proline rich sequence of the cytoplasmic domain of IL 5R alpha , which is conserved among the alpha chains of IL 5R , IL 3R , and GM CSF receptor ( GM CSFR ) , was found to be essential for the IL 5 induced proliferative response , expression of nuclear proto oncogenes such as c jun , c fos , and c myc , and tyrosine phosphorylation of cellular proteins including JAK 2 protein tyrosine kinase . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
The role of the lyn product ( p53 / p56lyn ) , a membrane associated protein tyrosine kinase in the signaling pathway used by granulocyte macrophage CSFR ( GM CSFR ) was investigated by using the GM CSF dependent human megakaryoblastic leukemia cell line M 07e . ^^^ M 07e cells express GM CSFR and are dependent on GM CSF for survival and proliferation in vitro . ^^^ The formation of GM CSF / GM CSFR / lyn signaling complexes was verified in an autoradiographic study with anti lyn IP of M 07e cells that had been bound with 125I labeled recombinant human ( rh ) GM CSF . ^^^ Finally , the interaction of Lyn kinase with the GM CSFR complexes was further corroborated using anti GM CSF ( G 133 ) mAb , which coimmunoprecipitated both the p 120 beta subunit and lyn product in the lysates of M 07e cells that had been bound with rhGM CSF before cell lysis . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Activation of GM CSF receptor ( GMR ) triggers two distinct cytoplasmic signalling pathways , JAK 2 and Ras , and is sufficient to maintain proliferation of growth factor dependent cell lines . ^^^ Shc proteins are phosphorylated upon activation of GMR and may be involved in the transmission of GM CSF signals to Ras . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
This paper examines the structure , expression , and sequence of the GM CSF receptor alpha chain gene ( GMR alpha ) in three primary AML M 2 , XO samples and one AML M 2 , XO cell line . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
It is generally believed that the high affinity receptor is responsible for the multiple functions of GM CSF and that the isolated alpha subunit ( GMR alpha ) does not transduce a signal . ^^^ Xenopus laevis oocytes injected with RNA encoding human GMR alpha expressed up to 10 ( 10 ) low affinity sites for GM CSF ( Kd = 6 nM ) . ^^^ GM CSF binding to the alpha subunit expressed in Xenopus oocytes caused activation of 2 deoxyglucose transport through endogenous glucose transporters . 2 Deoxyglucose transport was stimulated by similar low concentrations of GM CSF in HL 60 leukemia cells as well as normal human neutrophils and Xenopus oocytes expressing GMR alpha . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
The GM CSF receptor ( GM CSFR ) is composed of alpha and beta subunits . ^^^ We have examined the dynamic regulation of high and low affinity GM CSFR expression in neutrophils ( 1100 + / 200 R / cell , KD 50 + / 15 pM ) and a GM CSF dependent human leukaemic cell line , TF 1 ( 2000 + / 450 R / cell KD 15 + / 5 pM ) and 8600 + / 1150 R / cell KD 1 . 8 + / 0 . 3 nM ) . ^^^ TF 1 cells , but not neutrophils , were able to re express GM CSFR following removal of GM CSF from medium . ^^^ Therefore , proliferation of TF 1 cells required the continued presence of GM CSF at a time when there was no detectable surface high affinity GM CSFR . ( ABSTRACT TRUNCATED AT 400 WORDS ) . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Interestingly , each GF induces the transactivation of the R ( s ) for the `` distal ' ' GF ( s ) : ( 1 ) IL 6 induces transactivation of IL 3R , but not of GM CSFR / EpR ; ( 2 ) IL 3 causes a rapid upmodulation of GM CSFR / EpR ( `` pure ' ' progenitors treated with IL 3 show upmodulation of GM CSFR alpha chain mRNA by reverse transcriptase polymerase chain reaction ) ; whereas ( 3 ) GM CSF induces the transactivation of the EpR . ^^^ Finally , Ep , GM CSF , and IL 3 do not modulate the expression of the `` proximal ' ' HGFRs ( ie , GM CSFR / IL 3R / IL 6R , IL 3R / IL 6R , and IL 6R , respectively ) . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Inhibition of 125I GM CSF binding to the GM CSFR alpha chain was demonstrated by mAb which mapped to the middle third of the third alpha helix ( amino acids 78 83 ) , the distal two thirds of the third alpha helix and the initial 7 residues of the loop between the third and fourth helix ( amino acids 78 94 ) , and the extreme carboxy terminus . ^^^ No inhibition of binding occured with an antibody whose domain begins in the first beta pleated sheet ( amino acids , 39 43 ) , continues through the second helix ( amino acids 55 64 ) and into the proximal third of the third helix ( to amino acid 77 ) . mAb which mapped to the distal half of the fourth helix and the carboxy terminal tail ( amino acids 110 127 ) increased the binding of 125I GM CSF to GM CSFR alpha . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
The genes encoding the IL 5 receptor alpha chain and the common beta chain reside on chromosome 3 and 22 respectively , while the GM CSF receptor alpha chain gene ( CSF2RA ) has been mapped to the pseudoautosomal region ( PAR ) of the sex chromosomes , which is a 2 . 6 Mb stretch of homologous sequence at the tips of the short arms within which a single obligatory recombination occurs during male meiosis . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
The high affinity GM CSF receptor ( GMR ) consists of two transmembrane anchored subunits ; a ligand binding alpha subunit ( transmembrane GMRalpha ) and a signal transducing beta subunit ( GMRbeta ) , both of which belong to the cytokine receptor superfamily . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Although the majority of fLC were positive for the alpha chain of GM CSF receptor ( GM CSFR ) , the beta chain of GM CSFR was detected only on 15 % of CD1a+ cells . fLC were also positive for IL 1 receptor ( IL 1R ) type 1 , IL 1R type 2 , 75 , 000 molecular weight TNF receptor ( TNFR ) and interferon gamma receptor ( IFN gamma R ) . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
OBJECTIVE : This study was conducted to investigate the expression of alpha IL 5 and alpha GM CSF receptors ( alpha IL5r and alpha GM CSFr ) mRNA in the paranasal sinus mucosa of atopic and nonatopic subjects with chronic sinusitis compared to controls . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
We report that the human ANT 3 ADP / ATP translocase gene is a pseudoautosomal gene located proximal to the GM CSF receptor alpha chain gene ( CSF2RA ) . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Northern and reverse transcriptase polymerase chain reaction analysis of GM CSF R alpha and beta c ( KH 97 ) transcripts did not provide indications for the involvement of GM CSF R splice variants in the formation of the intermediate affinity GM CSFR complex . ^^^ These results are discussed in view of the concept that increasing expression of beta c subunits may cause alternative oligomerization of the GM CSF R alpha and beta c subunits resulting in the formation of intermediate rather than high affinity GM CSFR alpha . beta c complexes . . ^^^ In COS cell transfectants with increasing amounts of beta c in the presence of a fixed number of GM CSF R alpha chains , the high affinity GM CSF binding converted into intermediate affinity GM CSF binding . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
GMR alpha and IL 3R alpha were expressed on GM CSF and IL 3 responsive cell lines , such as TF 1 and UT 7 , whereas the expression levels were much lower on UT 7E , a GM CSF and IL 3 unresponsive subline of UT 7 . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
In this paper , we describe a physical association between the tyrosine phosphorylated GM CSF receptor ( GMR ) beta c chain and Shc in vivo . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Thus , the differentiation of BCL 1 B20 into IgM secreting cells can be equally supported by either IL 3 or GM CSF , suggesting that intracellular signaling through IL 5R can be replaced by signaling from IL 3R and GM CSFR . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
This rAb and a peptide derived from the rAb first complementarity determining region ( CDR ) sequence bind to a monoclonal anti GM CSF antibody that mimics the GM CSFR alpha chain , compete with GM CSF for binding to GM CSF receptor alpha chain ( GM CSFR alpha ) , and are specific biological antagonists . ^^^ Molecular modeling of the rAb suggests structural similarity with a site previously implicated in GM CSF binding to the GM CSFR alpha . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
We found by reverse transcriptase polymerase chain reaction ( RT PCR ) that hOB cells express constitutively both GM CSF and the alpha and beta chains of the GM CSF receptor ( GMR ) . ^^^ Immunocytochemistry showed that serum starved hOB cells express both GM CSF and GMR alpha chain . ^^^ The findings that human osteoblastic cells produce GM CSF and express functional GMR constitutively and that suppression of endogenous GM CSF results in inhibition of cell growth demonstrate that GM CSF is involved as an autocrine growth factor for human osteoblastic cells . . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
We now report that ECs express the alpha and beta subunits of GM CSF receptor ( GM CSFR ) , and that GM CSF is able to activate the Janus kinase ( JAK ) 2 , a member of the cytosolic tyrosine kinase family , which is known to mediate signals of several non tyrosine kinase receptors . ^^^ The effect is specific as inferred by the lack of effect of heat inactivated GM CSF or neutralized by specific antibodies and by the finding that interleukin 5 , which utilizes a specific alpha chain and the same beta chain of GM CSFR , does not phosphorylate JAK 2 . ^^^ Furthermore , we show that the amount of JAK 2 physically associated with GM CSFR beta chain is increased after GM CSF stimulation and that GM CSF triggers both beta chain and JAK 2 tyrosine phosphorylation . ^^^ Taken together , these results suggest that biologic activities of GM CSF in vascular endothelium may , in part , be elicited by GM CSFR mediated JAK 2 activation . . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Co expression of human GMR alpha chain and wild type human beta c in two murine leukaemic cell lines ( M 1 and WEHI 3B D+ ) conferred the ability to terminally differentiate into macrophages when stimulated with human GM CSF . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
We screened RNA from six patients with JCML for mutations in the GM CSF receptor ( GM CSFR ) coding sequence using RT PCR SSCP . ^^^ Polymorphisms of the GM CSFR alpha and beta chains are common , but pathogenic point mutations of the GM CSFR are infrequent in patients with JCML and are unlikely to be involved in the hypersensitivity to GM CSF demonstrated by progenitor cells . . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
OBJECTIVE : This study was carried out to investigate the expression of alpha GM CSF receptor ( alpha GM CSFr ) messenger RNA and protein in the bronchial mucosa of patients with intrinsic or atopic asthma and of control subjects and to correlate the expression of alpha GM CSFr to the number of EG2+ cells ( eosinophils ) and CD68+ cells ( macrophages ) and pulmonary function . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
The GM CSF receptor ( GMR ) alpha chain confers low affinity binding only ( 5 10 nM ) , whereas the other chain , betac , does not bind GM CSF by itself but confers high affinity binding when associated with GMR alpha ( 25 100 pM ) . ^^^ Third , removal of the charge at position R 280 of GMR alpha introduced a 10 fold decrease in the association rate constant and only a 2 fold change in the dissociation rate constant , suggesting that R 280 is implicated in ligand recognition , possibly through interaction with Asp 112 of GM CSF . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
We found that monoclonal antibodies to the GM CSF receptor alpha chain ( GMR alpha ) and to the common beta chain of the GM CSF , IL 3 , and IL 5 receptors ( beta ( c ) ) immunoprecipitated both GMR alpha and beta ( c ) from the surface of primary myeloid cells , myeloid cell lines , and transfected cells in the absence of GM CSF . ^^^ Significantly , receptor phosphorylation experiments showed that not only GM CSF but also IL 3 and IL 5 stimulated the phosphorylation of GMR alpha associated beta ( c ) . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
We describe here expression defects of betac or betac plus GM CSF receptor alpha chain ( GM CSFR alpha ) in 3 pediatric patients with AML and PAP symptoms . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
GM CSF receptor ( GM CSFR ) and c Fes tyrosine kinase were also activated upon GM CSF stimulation . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
In this regard the AA have evaluated in primary cultures of human gengival normal fibroblasts ( PG 1 cells ) : a ) the expression of GM CSF receptor ( GM CSFR ) ( alfa unit ) on the cell surface ; b ) the in vitro effects of different doses of GM CSF on the GM CSFR expression and on the proliferation and activity of fibroblasts . ^^^ PG 1 cells have been stimulated in vitro with different concentrations of GM CSF ( 10 , 50 , 80 , 100 and 150 ng / ml ) using promonocytic cell line U 937 as positive control for GM CSFR expression . ^^^ At high GM CSF concentration ( 80 ng / ml ) the AA observed : 1 ) A marked increase of GM CSFR expression evaluated as fluorescence intensity ( about three fold in respect to the controls ) ; 2 ) Maximal increase of PG 1 cells proliferation . ^^^ These results demonstrate : 1 ) The presence of GM CSFR on the surface of fibroblasts ; 2 ) The proliferation and the synthesis activity of these cells ( in vitro ) are modulated by different concentration of GM CSF . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Despite the heightened growth responses of the motheaten macrophages to GM CSF , there were no marked differences between motheaten macrophages and normal controls in GM CSF induced phosphorylation of GM CSFR beta , Jak 2 , STAT 5 and MAPK , indicating that these molecules are not major HCP substrates in GM CSF signaling . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
A quantitative analysis of expression levels of GM CSF receptors was performed by flow cytometry in different disease categories , ie AML ( n = 72 ) , ALL ( n = 18 ) , and MDS ( n = 12 ) , as well as 12 healthy volunteers , using three different unconjugated GM CSF / R monoclonal antibodies ( McAbs ) ( HGM CSFR ( CD 116 ) , M5D12 , 4B5F5 ) , and appropriate standards . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
The level of GM CSF receptor ( GM CSFR ) expression was determined by flow cytometry after labelling with specific antibodies for the alpha and beta subunits . ^^^ Although the toxicity to DT GM CSF was specifically mediated by the GM CSFR , no correlation was found between the level of expression of the GM CSFR alpha or beta subunit and the sensitivity for DT GM CSF . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Freshly prepared Langerhans cells ( fLC ) expressed the mRNA for receptors for M CSF ( cfms ) , GM CSF ( GM CSFR ) , and TNF alpha ( TNFRII ) . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Expression of GM CSF receptor ( GM CSFR ) alpha and beta mRNA subunits by cumulus oocyte complexes and mural granulosa cells was examined using RT PCR . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
To investigate the mechanisms of the defective response in MDS , we examined expression levels of GM CSF receptor alpha ( GMR alpha ) and common beta ( beta c ) subunits on CD 16 ( + ) neutrophils , CD 14 ( + ) monocytes and CD 3 ( + ) T cells from 26 MDS patients and 10 healthy controls using flow cytometry . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
The proportion of PCM positive for the GM CSF receptor ( GM CFSR ) alpha chain was examined by flow cytometric analysis and the expression of GM CSFR alpha chain mRNA in PCM was examined by reverse transcription / polymerase chain reaction analysis . ^^^ CONCLUSION : These findings suggest that GM CSF may stimulate GM CSFR alpha chain positive PCM and that these PCM may augment LAK activities developed by PLY and PBL . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Therefore we investigated ( 1 ) the expression levels of the GM CSF receptor ( GM CSFR ) constituents in three malignant plasma cell lines and in native malignant plasma cells , ( 2 ) the ability of the receptor to mediate common signalling pathways regulating proliferation and cell survival in malignant plasma cell lines , and ( 3 ) the effects of GM CSF on tumour cell biology . ^^^ We conclude that ( 1 ) the functional GM CSFR is commonly expressed on malignant plasma cells and that ( 2 ) GM CSF promotes the clonal expansion of myeloma cells by inhibiting spontaneous apoptosis and promoting DNA synthesis . . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
The high affinity GM CSF receptor ( GMR ) consists of two transmembrane anchored subunits : a ligand specific , low affinity subunit ( GMRalpha ) ; and a signal transducing beta subunit ( GMRbeta ) . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
We examined GM CSF / GM CSF receptor ( GM CSFR ) gene expression in 20 human non small cell lung cancer ( NSCLC ) xenografts . ^^^ A NSCLC xenograft ( LC 11 JCK ) , expressed GM CSFR but not GM CSF , and showed more rapid growth in GM Tg SCID than non GM CSF transgenic SCID mice ( non Tg SCID ) . ^^^ GM CSF gene expression was detected in 48 of 90 ( 53 % ) primary NSCLC human specimens and GM CSFR gene expression was detected in 42 specimens ( 47 % ) . ^^^ GM CSF expression was detected in 13 of 30 squamous cell carcinoma specimens ( 43 % ) and GM CSFR expression was detected in 10 specimens ( 33 % ) . ^^^ Patients with squamous cell carcinoma coexpressing GM CSF and GM CSFR showed significantly poorer prognosis than those expressing neither GM CSF nor GM CSFR ( P < 0 . 05 , Cox Mantel test ) . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Binding of GM CSF activates at least one receptor associated tyrosine kinase , JAK 2 , and rapidly induces tyrosine phosphorylation of the GMR beta c chain ( GMR beta ) , but not the GMR alpha chain ( GMR alpha ) . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
By transient transfection experiments with HIV 1 LTR reporter constructs , we showed that strong LTR mediated activation was induced by GM CSF in mouse Ba / F3 cells expressing human GM CSF receptors ( GM CSFR ) . ^^^ Further analyses of GM CSFR mutants suggested that some other unknown signalling pathway also participates in GM CSF induced HIV 1 LTR activation . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Activating monocytes with soluble CD 40 ligand ( CD40L ) led to accelerated differentiation toward mature CD 83 ( + ) DCs with up regulated human leukocyte antigen DR , costimulatory molecules and CD 116 ( GM CSF receptor ) , and down regulation of molecules involved in antigen capture . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
We show that in vivo thymic DC express E cadherin , CLA , CD 4 , CD 38 , CD 40 , CD 44 , and granulocyte macrophage colony stimulating factor R ( GM CSF R ; CD 116 ) but no CD1a . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
In this work we demonstrated the expression of the receptor for GM CSF ( GM CSFR ) on human normal skin fibroblasts from healthy subjects ( NFPC ) and on a human normal fibroblast cell line ( NHDF ) and we try to investigate the biological effects of this cytokine . ^^^ Human normal fibroblasts were cultured with different doses of GM CSF to study the effects of this factor on GM CSFR expression , on cell proliferation and adhesion structures . ^^^ The growth rate of fibroblasts from healthy donors ( NFPC ) is not augmented by GM CSF stimulation in spite of increased expression of the GM CSFR . ^^^ We suggest that the in vitro treatment with GM CSF can shift human normal fibroblasts towards a more differentiated state , due or accompanied by an increased expression of GM CSFR and that such `` differentiation ' ' is an important event induced by such cytokine . . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
The proportion of GM CSFR positive PBE and in vitro responsiveness of PBE to recombinant GM CSF were not different between the groups . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
GM CSF receptor ( GM CSFR ) was down regulated by GM CSF or phorbol 12 myristate 13 acetate in both of the normal controls and SLE patients , while this change was more remarkable in the latter . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
A number of residues located on the B and C helices of GM CSF are postulated to interact with the alpha chain of the GM CSF receptor ( GM CSFR ) . ^^^ Key residues involved in CDR 1 peptide / GM CSFR binding were identified by truncation and alteration of individual residues , while the structural elements required to antagonize the biological action of GM CSF were separately tested in binding and inhibitory activity assays of multiple cyclic analogues . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
The expression of MHC class 2 molecules , co stimulatory molecules and the GM CSF receptor ( CD 116 ) on the ganglioside treated DC was significantly reduced . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Granulocyte macrophage colony stimulating factor ( GM CSF ) up regulates HLA DR expression on monocytes via the GM CSF receptor ( GM CSFr ) through a transcriptional mechanism involving the class 2 transactivator factor ( CIITA ) . ^^^ Granulocyte macrophage colony stimulating factor ( GM CSF ) up regulates HLA DR expression on monocytes via the GM CSF receptor ( GM CSFr ) through a transcriptional mechanism involving the class 2 transactivator factor ( CIITA ) . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
By Southern analysis of somatic cell hybrid panels , pulsed field gel electrophoresis ( PFGE ) , and fluorescence chromosomal in situ hybridization , we have mapped the cloned sequence for the IL 3 receptor alpha ( IL3RA ) to the 10 Y pseudoautosomal region at bands Xp22 . 3 and Yp11 . 3 , near the gene for the alpha subunit of the CSF 2 receptor ( CSF2RA ) . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Little is known about this signal transduction pathway and the role of the alpha subunit of the GM CSF receptor ( alpha GMR ) in modulating transporter activity . ^^^ We examined the function of phosphatidylinositol 3 kinase ( PI 3 kinase ) in GM CSF stimulated glucose uptake and found that PI 3 kinase inhibitors , wortmannin and LY 294002 , completely blocked the GM CSF dependent increase of glucose uptake in Xenopus oocytes expressing the low affinity alpha GMR and in human cells expressing the high affinity alpha beta GMR complex . ^^^ Experiments with a construct eliminating most of the intracellular portion of alpha GMR showed a 50 % reduction in GM CSF stimulated glucose uptake with residual activity blocked by wortmannin . ^^^ Catalase treatment abrogated GM CSF or anti alpha GMR antibody stimulated glucose uptake in alpha GMR expressing oocytes , and H ( 2 ) O ( 2 ) activated PI 3 kinase and led to some stimulation of glucose uptake in uninjected oocytes . ^^^ Human myeloid cell lines and primary explant human lymphocytes expressing high affinity GM CSF receptors responded to alpha GMR antibody with increased glucose uptake . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Most cell types , including hepatocytes and skeletal , smooth , and cardiac myocytes , also expressed the GM CSF receptor ( GMR ) but the relative tissue levels of GMR were not always proportional to Magmas . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
These data suggest that a majority of CLPs and a fraction of pro T cells possess plasticity for myelomonocytic differentiation that can be activated by ectopic GM CSF signals , supporting the hypothesis that the down regulation of GM CSFR is a critical event in producing cells with a lymphoid restricted lineage potential . . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
GM CSF is an important growth factor for cells of both the macrophage and granulocyte lineages and enhances effector functions of these cells via the heterodimeric GM CSF receptor ( GM CSFR ) . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
Constant exposure of M CSF and RANKL treated cells to GM CSF resulted in inhibition of osteoclast formation and the downregulation of CTSK and TRAP implicating the upregulation of CSF2R in a possible feedback inhibition of osteoclastogenesis . . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
GM CSF receptor ( GM CSFR ) expression was determined by Western blot of cell lysates . ^^^
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
NA
Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
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Interacting proteins: P04141 and P15509	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.66080149
High affinity GMR interacts with GM CSF in a species specific manner , therefore one can analyze the effects of hGMR signals on differentiation of mouse hemopoietic progenitors using hGM CSF . 0.66080149^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.73184538
The effects of granulocyte / macrophage colony stimulating factor ( GM CSF ) are mediated by interaction with its composite receptor ( GMR ) , which consists of a unique alpha subunit ( GMR alpha ) and a beta subunit ( GMR beta ) that is common to the receptors for GM CSF , interleukin 3 , and interleukin 5 . 0.73184538^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.94074055
Thus , GM CSF binds to its receptor CD 116 , and consequently activates Syk phosphorylation , which leads to induce CXCR 3 expression . gamma IP 10 and Mig can induce Syk , Cbl , and Cbl b phosphorylation in CD 34 ( + ) progenitors by means of CXCR 3 . gamma IP 10 or Mig has induced neither chemotaxis nor adhesion in GM CSF stimulated Cbl b blocked CD 34 ( + ) hemopoietic progenitors , whereas SDF 1alpha induces both chemotaxis and adhesion in these cells . 0.94074055^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :1.1111931
GM CSF binds to specific cellular receptors ( GM CSFR ) which belong to a recently described supergene family . 1.1111931^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.61821476
This technology has been applied to the study of interactions between human granulocyte macrophage colony stimulating factor ( GM CSF ) and its receptor ( GM CSFR ) . 0.61821476^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
FDCs in the follicular apical light zone expressed transforming growth factor beta receptor 2 ( TGF betaR 2 ) , granulocyte macrophage colony stimulating factor receptor alpha ( GM CSFRalpha ; CDw 116 ) , tumor necrosis factor receptor 1 ( TNFR 1 ; CD120a ) , interleukin 1 receptor 2 ( IL 1R 2 ; CDw121b ) , IL 2 receptor beta ( IL 2Rbeta ; CD 122 ) , IL 4 receptor ( IL 4R ; CDw 124 ) , and IL 6 receptor ( IL 6R ; CD 126 ) , among the 10 receptors examined . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Granulocyte macrophage colony stimulating factor ( GM CSF ) activates a broad range of myeloid cells through binding to high affinity receptors ( GM CSF R ) consisting of at least two distinct subunits , GM CSF R alpha and GM CSF R beta . ^^^ Out of a panel of various cytokines and factors tested , only interferon gamma ( IFN gamma ) affected the expression of one of the GM CSF R subunit genes by increasing the GM CSF R beta mRNA expression threefold to sixfold with no effect on GM CSF R alpha . ^^^ Granulocyte macrophage colony stimulating factor ( GM CSF ) activates a broad range of myeloid cells through binding to high affinity receptors ( GM CSF R ) consisting of at least two distinct subunits , GM CSF R alpha and GM CSF R beta . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
In view of the recent isolation of a cDNA , designated GM CSF R beta , that confers high affinity binding of GM CSF in cotransfection experiments with the low affinity receptor , we suggest that the previously isolated low affinity receptor be designated GM CSF R alpha 1 and the one described in this report be designated GM CSF R alpha 2 . . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Demonstration of the low affinity alpha subunit of the granulocyte macrophage colony stimulating factor receptor ( GM CSF R alpha ) on human trophoblast and uterine cells . ^^^ In this study we have performed a detailed immunohistological localization of the low affinity GM CSF receptor ( GM CSF R alpha ) in human first trimester implantation site and non pregnant endometrium . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Two distinct cDNA clones , corresponding to alternative mRNA splicing variants of the alpha subunit of the granulocyte macrophage colony stimulating factor receptor ( GM CSF R alpha ) were isolated from human blood mononuclear cells by RT PCR . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Northern and reverse transcriptase polymerase chain reaction analysis of GM CSF R alpha and beta c ( KH 97 ) transcripts did not provide indications for the involvement of GM CSF R splice variants in the formation of the intermediate affinity GM CSFR complex . ^^^ In COS cell transfectants with increasing amounts of beta c in the presence of a fixed number of GM CSF R alpha chains , the high affinity GM CSF binding converted into intermediate affinity GM CSF binding . ^^^ These results are discussed in view of the concept that increasing expression of beta c subunits may cause alternative oligomerization of the GM CSF R alpha and beta c subunits resulting in the formation of intermediate rather than high affinity GM CSFR alpha . beta c complexes . . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Activation of GM CSF receptor ( GMR ) triggers two distinct cytoplasmic signalling pathways , JAK 2 and Ras , and is sufficient to maintain proliferation of growth factor dependent cell lines . ^^^ Shc proteins are phosphorylated upon activation of GMR and may be involved in the transmission of GM CSF signals to Ras . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
This paper examines the structure , expression , and sequence of the GM CSF receptor alpha chain gene ( GMR alpha ) in three primary AML M 2 , XO samples and one AML M 2 , XO cell line . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
To determine the expression and function of the granulocyte macrophage colony stimulating factor ( GM CSF ) receptor alpha chain ( GMR alpha ) during hematopoiesis and on leukemic cells , monoclonal antibodies were raised by immunizing mice with cells expressing high levels of human GMR alpha . ^^^ Anti GMR alpha inhibited the GM CSF dependent growth of cell lines and normal bone marrow cells and inhibited the binding of iodinated GM CSF to its receptor . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Interleukin ( IL ) 3 , granulocyte macrophage colony stimulating factor , and IL 5 receptors ( IL 3R , GMR , and IL 5R ) are composed of the alpha chain specific to each and the common beta chain , and both the alpha and beta subunits are members of the cytokine receptor superfamily . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
To investigate the molecular basis of this , we generated a clone of transfected NIH3T3 cells that constitutively expressed the human granulocyte macrophage colony stimulating factor receptor ( GM CSF R ) beta chain and inducibly expressed the human GM CSF R alpha chain . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
We identified a truncated , soluble form of the low affinity GM CSF receptor ( GMR ) in chorio carcinoma cells . ^^^ Expression of the soluble GMR cDNA in murine psi AM cells as well as GM CSF dependent myeloid 32Dc13 cells produced a secreted protein that retained its capacity to bind GM CSF in solution . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Granulocyte macrophage colony stimulating factor receptor ( GMR ) is composed of two distinct subunits alpha and beta , and the cytoplasmic domains of both subunits are essential to transduce signals . ^^^ Granulocyte macrophage colony stimulating factor receptor ( GMR ) is composed of two distinct subunits alpha and beta , and the cytoplasmic domains of both subunits are essential to transduce signals . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
The granulocyte / macrophage colony stimulating factor ( GM CSF ) receptor ( GMR ) is a heterodimeric receptor expressed by myeloid lineage cells . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
While the primary targets for granulocyte macrophage colony stimulating factor ( GM CSF ) are hematopoietic precursors and mature myeloid cells , GM CSF receptors ( GMR ) are also found on normal tissues including placenta , endothelium , and oligodendrocytes as well as certain malignant cells . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
To identify domains in hematopoietic growth factor receptors that are important for signal transduction , a hybrid receptor ( GMER ) was constructed by splicing the DNA of the entire extracellular and transmembrane domains of the human granulocyte macrophage colony stimulating factor ( GM CSF ) receptor alpha 2 subunit ( GMR ) to the cytoplasmic domain of the murine erythropoietin receptor ( mEpoR ) . ^^^ Human GM CSF binding to Ba / F3 cells that expressed GMER showed a kd of 3 . 0 nmol / L and 475 binding sites / cell , while the same cells that expressed GMR had 300 sites / cell and a kd of 3 . 5 nmol / L . ^^^ Expression of GMR plus the human GM CSF receptor beta chain in the same cell line also resulted in human GM CSF stimulated proliferation ; however , cell surface glycophorin was not detected . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Only little is known about the expression of a functional granulocyte macrophage colony stimulating factor receptor ( GMR ) and its ligands in human colon carcinoma cell lines . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
It is generally believed that the high affinity receptor is responsible for the multiple functions of GM CSF and that the isolated alpha subunit ( GMR alpha ) does not transduce a signal . ^^^ Xenopus laevis oocytes injected with RNA encoding human GMR alpha expressed up to 10 ( 10 ) low affinity sites for GM CSF ( Kd = 6 nM ) . ^^^ GM CSF binding to the alpha subunit expressed in Xenopus oocytes caused activation of 2 deoxyglucose transport through endogenous glucose transporters . 2 Deoxyglucose transport was stimulated by similar low concentrations of GM CSF in HL 60 leukemia cells as well as normal human neutrophils and Xenopus oocytes expressing GMR alpha . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
For the samples in groups A and B , RA affected neither G CSF receptor ( GR ) nor GM CSF receptor ( GMR ) . ^^^ This finding supports the hypothesis that the increase of GMR is one of the causes of the stimulative effects of RA on cells cultured with GM CSF in group C . . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Granulocyte macrophage colony stimulating factor ( GM CSF ) binds to the high affinity GM CSF receptor ( GMR ) consisting of alpha and beta subunits and induces rapid tyrosine phosphorylation , activation of early response genes , and proliferation of hematopoietic cells . ^^^ Granulocyte macrophage colony stimulating factor ( GM CSF ) binds to the high affinity GM CSF receptor ( GMR ) consisting of alpha and beta subunits and induces rapid tyrosine phosphorylation , activation of early response genes , and proliferation of hematopoietic cells . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
The cytoplasmic domain of the GM CSF receptor alpha subunit ( GMR alpha ) comprises 54 amino acids that have been shown to be important for signal transduction through the beta chain . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
GMR alpha and IL 3R alpha were expressed on GM CSF and IL 3 responsive cell lines , such as TF 1 and UT 7 , whereas the expression levels were much lower on UT 7E , a GM CSF and IL 3 unresponsive subline of UT 7 . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
The high affinity and functional granulocyte macrophage colony stimulating factor receptor ( GMR ) is composed of two distinct subunits , alpha and beta ; and the cytoplasmic domain of the beta subunit is essential to transduce growth promoting signals . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
In this paper , we describe a physical association between the tyrosine phosphorylated GM CSF receptor ( GMR ) beta c chain and Shc in vivo . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
The high affinity GM CSF receptor ( GMR ) consists of two transmembrane anchored subunits ; a ligand binding alpha subunit ( transmembrane GMRalpha ) and a signal transducing beta subunit ( GMRbeta ) , both of which belong to the cytokine receptor superfamily . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
The high affinity receptor for granulocyte macrophage colony stimulating factor ( GMR ) comprises at least 2 distinct subunits , alpha and beta common ( beta c ) , whereas the normal erythropoietin receptor ( nEpoR ) comprises only one known subunit . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Granulocyte macrophage colony stimulating factor ( GM CSF ) receptors ( GMR ) are expressed on myeloid cells throughout their maturational sequence . ^^^ Granulocyte macrophage colony stimulating factor ( GM CSF ) receptors ( GMR ) are expressed on myeloid cells throughout their maturational sequence . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
The receptor for human granulocyte macrophage ( GM ) CSF ( GMR ) is a heterodimer , consisting of an alpha chain ( GMR alpha ) and a beta chain ( GMR beta ) . ^^^ While GMR alpha is capable of binding GM CSF , GMR beta is necessary for signal transduction . ^^^ We have recently demonstrated that tyrosine 750 ( Y 750 ) in GMR beta is a site of GM CSF induced phosphorylation and this site may contribute to the maintenance of cellular viability in response to GM CSF . ^^^ To investigate possible contributions made by additional GMR beta cytoplasmic tyrosine residues to receptor function , we mutated other selected tyrosine residues to phenylalanine and tested for any defects in signaling . in the present study , we show that Y 577 is required for phosphorylation of Shc and an Shc associated p 140 in response to GM CSF . ^^^ Interestingly , mutation of Y 750 reduced detectable GM CSF induced tyrosine phosphorylation of GMR beta , suggesting that the reduction of Shc phosphorylation associated with that mutant might be actually due to a failure to phosphorylate Y 577 . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
We recently demonstrated that a chimeric receptor ( GMER ) that is composed of the extracellular and transmembrane domains of the human granulocyte macrophage colony stimulating factor ( GM CSF ) receptor alpha chain ( GMR alpha ) and the cytoplasmic domain of the murine erythropoietin receptor mEpoR binds hGM CSF with low affinity ( 3 nM ) and confers both proliferative and differentiation signals to stably transfected murine Ba / F3 cells . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
We found by reverse transcriptase polymerase chain reaction ( RT PCR ) that hOB cells express constitutively both GM CSF and the alpha and beta chains of the GM CSF receptor ( GMR ) . ^^^ Immunocytochemistry showed that serum starved hOB cells express both GM CSF and GMR alpha chain . ^^^ The findings that human osteoblastic cells produce GM CSF and express functional GMR constitutively and that suppression of endogenous GM CSF results in inhibition of cell growth demonstrate that GM CSF is involved as an autocrine growth factor for human osteoblastic cells . . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Co expression of human GMR alpha chain and wild type human beta c in two murine leukaemic cell lines ( M 1 and WEHI 3B D+ ) conferred the ability to terminally differentiate into macrophages when stimulated with human GM CSF . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
The GM CSF receptor ( GMR ) alpha chain confers low affinity binding only ( 5 10 nM ) , whereas the other chain , betac , does not bind GM CSF by itself but confers high affinity binding when associated with GMR alpha ( 25 100 pM ) . ^^^ Third , removal of the charge at position R 280 of GMR alpha introduced a 10 fold decrease in the association rate constant and only a 2 fold change in the dissociation rate constant , suggesting that R 280 is implicated in ligand recognition , possibly through interaction with Asp 112 of GM CSF . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
We found that monoclonal antibodies to the GM CSF receptor alpha chain ( GMR alpha ) and to the common beta chain of the GM CSF , IL 3 , and IL 5 receptors ( beta ( c ) ) immunoprecipitated both GMR alpha and beta ( c ) from the surface of primary myeloid cells , myeloid cell lines , and transfected cells in the absence of GM CSF . ^^^ Significantly , receptor phosphorylation experiments showed that not only GM CSF but also IL 3 and IL 5 stimulated the phosphorylation of GMR alpha associated beta ( c ) . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
The granulocyte macrophage colony stimulating factor ( GM CSF ) receptor ( GMR ) is a heterodimeric receptor expressed by myeloid lineage cells . ^^^ Binding of GM CSF activates at least one receptor associated tyrosine kinase , JAK 2 , and rapidly induces tyrosine phosphorylation of the GMR betac chain ( GMRbeta ) , but not the GMR alpha chain ( GMRalpha ) . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
The high affinity human granulocyte macrophage colony stimulating factor ( GM CSF ) receptor ( GMR ) consists of an alpha ( GMRalpha ) and a common beta ( betac ) subunit . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
The high affinity GM CSF receptor ( GMR ) consists of two transmembrane anchored subunits : a ligand specific , low affinity subunit ( GMRalpha ) ; and a signal transducing beta subunit ( GMRbeta ) . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Binding of GM CSF activates at least one receptor associated tyrosine kinase , JAK 2 , and rapidly induces tyrosine phosphorylation of the GMR beta c chain ( GMR beta ) , but not the GMR alpha chain ( GMR alpha ) . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
To analyze the function of each subunit of the receptor for granulocyte macrophage colony stimulating factor ( GM CSF ) , GMR , we previously generated a single chain chimeric receptor by fusion of the extracellular and transmembrane domain from the alpha subunit ( alpha GMR ) to the intracellular part of the beta subunit ( beta GMR ) introducing an additional glutamate residue at the fusion site ( alpha / beta GMR ) . ^^^ We demonstrated the capacity of alpha / beta GMR to bind GM CSF with low affinity and to induce GM CSF dependent activation of tyrosine kinase activity and proliferation in transfected Ba / F3 cells . ^^^ None of these mutants was capable of supporting GM CSF dependent proliferation ; however , when beta GMR was coexpressed , GM CSF mediated short and long term proliferation . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
The granulocyte macrophage colony stimulating factor ( GM CSF ) receptor ( GMR ) is composed of two chains that belong to the superfamily of cytokine receptors typified by the growth hormone receptor . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Granulocyte macrophage colony stimulating factor ( GM CSF ) is an important hematopoietic cytokine that exerts its effects by interaction with the GM CSF receptor ( GMR ) on the surface of responsive cells . ^^^ Granulocyte macrophage colony stimulating factor ( GM CSF ) is an important hematopoietic cytokine that exerts its effects by interaction with the GM CSF receptor ( GMR ) on the surface of responsive cells . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
To investigate the mechanisms of the defective response in MDS , we examined expression levels of GM CSF receptor alpha ( GMR alpha ) and common beta ( beta c ) subunits on CD 16 ( + ) neutrophils , CD 14 ( + ) monocytes and CD 3 ( + ) T cells from 26 MDS patients and 10 healthy controls using flow cytometry . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
The receptors for human granuloctye macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) , and IL 5 are composed of a ligand specific alpha chain ( eg , alpha GM CSF receptor [ alpha GMR ] ) and a common beta subunit ( beta GMR ) . ^^^ In these cells , coexpression of beta GMR ( IT ) inhibits GM CSF mediated survival and proliferation in a GM CSF concentration dependent manner . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Most cell types , including hepatocytes and skeletal , smooth , and cardiac myocytes , also expressed the GM CSF receptor ( GMR ) but the relative tissue levels of GMR were not always proportional to Magmas . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
The cytokine granulocyte macrophage colony stimulating factor ( GM CSF ) regulates hematopoiesis and the function of mature host defense cells through the GM CSF receptor ( GMR ) , which is composed of alpha ( alphaGMR ) and beta ( betaGMR ) subunits . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
The granulocyte macrophage colony stimulating factor receptor alpha chain gene ( GM CSF R ) has recently been localised to the pseudoautosomal region of the sex chromosomes . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Affinity purification of human granulocyte macrophage colony stimulating factor receptor alpha chain . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Molecular cloning of a soluble form of the granulocyte macrophage colony stimulating factor receptor alpha chain from a myelomonocytic cell line . ^^^ To analyze the molecular and functional characteristics of a soluble form of the granulocyte macrophage colony stimulating factor receptor alpha chain ( sGM CSFR alpha ) , and analyze transcript expression in immune cells and the cellular constituents of rheumatoid arthritis synovial tissue . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Little is known about this signal transduction pathway and the role of the alpha subunit of the GM CSF receptor ( alpha GMR ) in modulating transporter activity . ^^^ We examined the function of phosphatidylinositol 3 kinase ( PI 3 kinase ) in GM CSF stimulated glucose uptake and found that PI 3 kinase inhibitors , wortmannin and LY 294002 , completely blocked the GM CSF dependent increase of glucose uptake in Xenopus oocytes expressing the low affinity alpha GMR and in human cells expressing the high affinity alpha beta GMR complex . ^^^ Experiments with a construct eliminating most of the intracellular portion of alpha GMR showed a 50 % reduction in GM CSF stimulated glucose uptake with residual activity blocked by wortmannin . ^^^ Catalase treatment abrogated GM CSF or anti alpha GMR antibody stimulated glucose uptake in alpha GMR expressing oocytes , and H ( 2 ) O ( 2 ) activated PI 3 kinase and led to some stimulation of glucose uptake in uninjected oocytes . ^^^ Human myeloid cell lines and primary explant human lymphocytes expressing high affinity GM CSF receptors responded to alpha GMR antibody with increased glucose uptake . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
HRMCs were also negative for CD 116 ( granulocyte macrophage colony stimulating factor [ GM CSF ] receptor alpha ) , CD 123 ( interleukin [ IL ] 3Ralpha ) , CD121a ( IL 1R type 1 ) , CD 122 ( IL 2Rbeta ) , and CD 127 ( IL 7R ) and were also found to lack C5aR ( CD 88 ) . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
The inhibition of DC differentiation by RCC CM was preceeded by an induction of M CSF receptor ( M CSFR ; CD 115 ) and a loss of granulocyte macrophage colony stimulating factor receptor ( GM CSFR ; CD 116 ) expression at the surface of CD 34 ( + ) cells , two phenomenon reversed by anti IL 6 / IL 6R and anti M CSF antibodies , respectively . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
We show that in vivo thymic DC express E cadherin , CLA , CD 4 , CD 38 , CD 40 , CD 44 , and granulocyte macrophage colony stimulating factor R ( GM CSF R ; CD 116 ) but no CD1a . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
CD 116 ( granulocyte macrophage colony stimulating factor receptor ) . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
The expression of MHC class 2 molecules , co stimulatory molecules and the GM CSF receptor ( CD 116 ) on the ganglioside treated DC was significantly reduced . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Neutralizing and nonneutralizing monoclonal antibodies to the human granulocyte macrophage colony stimulating factor receptor alpha chain . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Self peptides derived from the carboxy terminal end of the breakpoint cluster region ( bcr ) protein , as well as several differentiation stage and tissue specific self antigens characteristic of early stages of myeloid differentiation , such as c fes , c pim , granulocyte macrophage colony stimulating factor receptor alpha chain , proteinase 3 , and cathepsin G , were identified . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Cells from all samples strongly expressed c kit , granulocyte colony stimulating factor receptor ( G CSFR ) , c fms ( macrophage colony stimulating factor receptor ) , interleukin 3 receptor alpha chain ( IL 3Ralpha ) , and granulocyte macrophage colony stimulating factor receptor alpha chain ( GM CSFRalpha ) , and these data corresponded well to the growth responsiveness to the cytokines . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Cytokine R detectable on HMC 1 but not MC were granulocyte macrophage colony stimulating factor ( GM CSFR ) alpha ( CD 116 ) , CD 40 , Apo 1 / FAS ( CD 95 ) and gp 130 ( CD 130 ) . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
A quantitative analysis of expression levels of GM CSF receptors was performed by flow cytometry in different disease categories , ie AML ( n = 72 ) , ALL ( n = 18 ) , and MDS ( n = 12 ) , as well as 12 healthy volunteers , using three different unconjugated GM CSF / R monoclonal antibodies ( McAbs ) ( HGM CSFR ( CD 116 ) , M5D12 , 4B5F5 ) , and appropriate standards . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Activating monocytes with soluble CD 40 ligand ( CD40L ) led to accelerated differentiation toward mature CD 83 ( + ) DCs with up regulated human leukocyte antigen DR , costimulatory molecules and CD 116 ( GM CSF receptor ) , and down regulation of molecules involved in antigen capture . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
CD 116 [ granulocyte macrophage colony stimulating factor receptor alpha ( GM CSFRalpha ) ] and CD 123 ( IL 3Ralpha ) were expressed on MC precursors on day 14 , but disappeared thereafter . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
They included colony stimulating factor receptor ( CSF2R ) , SRY ( TDF ) , ZFY , RPS4Y ( ribosome protein 4S ) , ADMLY ( Kallman syndrome ) , AMGL ( amelogenin , which we cloned ourselves ) and so on . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Constant exposure of M CSF and RANKL treated cells to GM CSF resulted in inhibition of osteoclast formation and the downregulation of CTSK and TRAP implicating the upregulation of CSF2R in a possible feedback inhibition of osteoclastogenesis . . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Arrangement and localization of the human GM CSF receptor alpha chain gene CSF2RA within the 10 Y pseudoautosomal region . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
The gene encoding the granulocyte macrophage colony stimulating factor receptor alpha subunit ( CSF2RA ) has previously been mapped to the pseudoautosomal region of the human sex chromosomes . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
The genes encoding the IL 5 receptor alpha chain and the common beta chain reside on chromosome 3 and 22 respectively , while the GM CSF receptor alpha chain gene ( CSF2RA ) has been mapped to the pseudoautosomal region ( PAR ) of the sex chromosomes , which is a 2 . 6 Mb stretch of homologous sequence at the tips of the short arms within which a single obligatory recombination occurs during male meiosis . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
By Southern analysis of somatic cell hybrid panels , pulsed field gel electrophoresis ( PFGE ) , and fluorescence chromosomal in situ hybridization , we have mapped the cloned sequence for the IL 3 receptor alpha ( IL3RA ) to the 10 Y pseudoautosomal region at bands Xp22 . 3 and Yp11 . 3 , near the gene for the alpha subunit of the CSF 2 receptor ( CSF2RA ) . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
We report that the human ANT 3 ADP / ATP translocase gene is a pseudoautosomal gene located proximal to the GM CSF receptor alpha chain gene ( CSF2RA ) . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
We examined the changes in mRNA expression of granulocyte macrophage colony stimulating factor receptor beta subunit ( GM CSFR beta subunit ) , which was a common subunit of a high affinity interleukin 3 receptor ( IL 3R ) and a high affinity GM CSFR , and interleukin 6 receptor ( IL 6R ) during megakaryocyte development in a human megakaryocytic leukemia cell line ( CMK ) which could proliferate and / or differentiate in the presence of 12 O tetradecanoylphorbol 13 acetate ( TPA ) , IL 3 , GM CSF , and IL 6 . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Chimaeric IL 2 receptor beta and gamma chains constructed with the heterodimeric extracellular regions of the granulocyte macrophage colony stimulating factor receptor ( GM CSFR ) also provided the IL 2R signal . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
We have therefore studied the coding sequence of the GM CSF receptor alpha chain ( GM CSFR alpha ) in patients with acute myeloid leukaemia ( AML ) and non AML controls using single strand conformation polymorphism ( SSCP ) analysis . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Flow cytometric analysis of five myeloma cell lines ( RPMI 8226 , ARH 77 , KMM 1 , U 266 , and Hs ) and myeloma cells freshly isolated from eight patients showed that interleukin 1 receptor ( IL 1R ) type 1 and type 2 , IL 2R alpha and beta chains , IL 4R , IL 6R , IL 7R , IL 8R , granulocyte macrophage colony stimulating factor receptor ( GM CSFR ) , c kit ( stem cell factor receptor [ SCFR ] ) , membrane bound stem cell factor ( MBSCF ) , and tumor necrosis factor ( TNF ) receptors type 1 and type 2 were not always detected on the myeloma cells . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
The membrane proximal proline rich sequence of the cytoplasmic domain of IL 5R alpha , which is conserved among the alpha chains of IL 5R , IL 3R , and GM CSF receptor ( GM CSFR ) , was found to be essential for the IL 5 induced proliferative response , expression of nuclear proto oncogenes such as c jun , c fos , and c myc , and tyrosine phosphorylation of cellular proteins including JAK 2 protein tyrosine kinase . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
The role of the lyn product ( p53 / p56lyn ) , a membrane associated protein tyrosine kinase in the signaling pathway used by granulocyte macrophage CSFR ( GM CSFR ) was investigated by using the GM CSF dependent human megakaryoblastic leukemia cell line M 07e . ^^^ M 07e cells express GM CSFR and are dependent on GM CSF for survival and proliferation in vitro . ^^^ The formation of GM CSF / GM CSFR / lyn signaling complexes was verified in an autoradiographic study with anti lyn IP of M 07e cells that had been bound with 125I labeled recombinant human ( rh ) GM CSF . ^^^ Finally , the interaction of Lyn kinase with the GM CSFR complexes was further corroborated using anti GM CSF ( G 133 ) mAb , which coimmunoprecipitated both the p 120 beta subunit and lyn product in the lysates of M 07e cells that had been bound with rhGM CSF before cell lysis . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Binding studies confirmed the presence of receptors for GM CSF ( GM CSFR ) on EC . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Whereas the human IL 3R alpha gene is tightly linked with the granulocyte macrophage colony stimulating factor receptor alpha subunit ( GM CSFR alpha ) gene in the pseudoautosomal region of the 10 and Y chromosomes , the mIL 3R alpha gene ( II3ra ) is located in the proximal region of mouse chromosome 14 , separated from the mouse GM CSFR alpha gene , which is on chromosome 19 . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
The results suggest a model of transactivation cascade for HGFR such as interleukin 6 receptor ( IL 6R ) , IL 3R , GM colony stimulating factor receptor ( GM CSFR ) , and erythropoietin receptor ( EpR ) , whereby each HGF upmodulates the R ( s ) for distal acting HGF ( s ) . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
The GM CSF receptor ( GM CSFR ) is composed of alpha and beta subunits . ^^^ We have examined the dynamic regulation of high and low affinity GM CSFR expression in neutrophils ( 1100 + / 200 R / cell , KD 50 + / 15 pM ) and a GM CSF dependent human leukaemic cell line , TF 1 ( 2000 + / 450 R / cell KD 15 + / 5 pM ) and 8600 + / 1150 R / cell KD 1 . 8 + / 0 . 3 nM ) . ^^^ TF 1 cells , but not neutrophils , were able to re express GM CSFR following removal of GM CSF from medium . ^^^ Therefore , proliferation of TF 1 cells required the continued presence of GM CSF at a time when there was no detectable surface high affinity GM CSFR . ( ABSTRACT TRUNCATED AT 400 WORDS ) . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Interestingly , each GF induces the transactivation of the R ( s ) for the `` distal ' ' GF ( s ) : ( 1 ) IL 6 induces transactivation of IL 3R , but not of GM CSFR / EpR ; ( 2 ) IL 3 causes a rapid upmodulation of GM CSFR / EpR ( `` pure ' ' progenitors treated with IL 3 show upmodulation of GM CSFR alpha chain mRNA by reverse transcriptase polymerase chain reaction ) ; whereas ( 3 ) GM CSF induces the transactivation of the EpR . ^^^ Finally , Ep , GM CSF , and IL 3 do not modulate the expression of the `` proximal ' ' HGFRs ( ie , GM CSFR / IL 3R / IL 6R , IL 3R / IL 6R , and IL 6R , respectively ) . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
The intracytoplasmic tail of the granulocyte macrophage colony stimulating factor receptor ( GM CSFR ) beta c chain is essential for the activation of ligand mediated signal transduction pathways in myeloid cells . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
The granulocyte macrophage colony stimulating factor receptor ( GM CSFR ) is composed of at least two chains ( alpha and beta ) . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Inhibition of 125I GM CSF binding to the GM CSFR alpha chain was demonstrated by mAb which mapped to the middle third of the third alpha helix ( amino acids 78 83 ) , the distal two thirds of the third alpha helix and the initial 7 residues of the loop between the third and fourth helix ( amino acids 78 94 ) , and the extreme carboxy terminus . ^^^ No inhibition of binding occured with an antibody whose domain begins in the first beta pleated sheet ( amino acids , 39 43 ) , continues through the second helix ( amino acids 55 64 ) and into the proximal third of the third helix ( to amino acid 77 ) . mAb which mapped to the distal half of the fourth helix and the carboxy terminal tail ( amino acids 110 127 ) increased the binding of 125I GM CSF to GM CSFR alpha . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
OBJECTIVE : This study was conducted to investigate the expression of alpha IL 5 and alpha GM CSF receptors ( alpha IL5r and alpha GM CSFr ) mRNA in the paranasal sinus mucosa of atopic and nonatopic subjects with chronic sinusitis compared to controls . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Thus , the differentiation of BCL 1 B20 into IgM secreting cells can be equally supported by either IL 3 or GM CSF , suggesting that intracellular signaling through IL 5R can be replaced by signaling from IL 3R and GM CSFR . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
This rAb and a peptide derived from the rAb first complementarity determining region ( CDR ) sequence bind to a monoclonal anti GM CSF antibody that mimics the GM CSFR alpha chain , compete with GM CSF for binding to GM CSF receptor alpha chain ( GM CSFR alpha ) , and are specific biological antagonists . ^^^ Molecular modeling of the rAb suggests structural similarity with a site previously implicated in GM CSF binding to the GM CSFR alpha . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
To determine whether heterodimeric PRLr complexes were capable of ligand induced signaling and cellular proliferation , chimeras consisting of the extracellular domain of either the alpha or beta subunit of human granulocyte macrophage colony stimulating factor receptor ( GM CSFr ) and the intracellular domain of the rat intermediate or short PRLr isoforms ( PRLr 1 or PRLr S ) were synthesized . ^^^ Because high affinity binding of GM CSF is mediated by the extracellular domain of one alpha and beta GM CSFr pair , use of GM CSFr / PRLr chimera specifically directed the dimerization of the PRLr intracellular domains within ligand receptor complexes . ^^^ When tested for their ability to proliferate in response to GM CSF , only chimeric transfectants expressing GM CSFr / PRLr 1 homodimers demonstrated significant [ 3H ] thymidine incorporation . ^^^ GM CSF stimulation of transfectants expressing either GM CSFr / PRLr S homodimers or GM CSFr / PRLr S+1 heterodimers failed to induce proliferation . ^^^ Consistent with these data , the GM CSF induced activation of two phosphotyrosine kinases , Jak 2 and Fyn , was observed only in homodimeric GM CSFr / PRLr 1 transfectants . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Although the majority of fLC were positive for the alpha chain of GM CSF receptor ( GM CSFR ) , the beta chain of GM CSFR was detected only on 15 % of CD1a+ cells . fLC were also positive for IL 1 receptor ( IL 1R ) type 1 , IL 1R type 2 , 75 , 000 molecular weight TNF receptor ( TNFR ) and interferon gamma receptor ( IFN gamma R ) . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
A flow cytometric method to quantify the Granulocyte macrophage colony stimulating factor receptor ( GM CSFr ) on human cells is described . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Despite the heightened growth responses of the motheaten macrophages to GM CSF , there were no marked differences between motheaten macrophages and normal controls in GM CSF induced phosphorylation of GM CSFR beta , Jak 2 , STAT 5 and MAPK , indicating that these molecules are not major HCP substrates in GM CSF signaling . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
All DDC were highly positive for granulocyte macrophage colony stimulating factor receptor ( GM CSFR ) alpha chain ( mAb hGM CSFR M 1 ; SC 06 ; SC 04 , and 8G6 ) and to a lesser extent for the common beta chain of GM CSFR , IL 3R and IL 5R ( mAb 3D7 ) . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
We now report that ECs express the alpha and beta subunits of GM CSF receptor ( GM CSFR ) , and that GM CSF is able to activate the Janus kinase ( JAK ) 2 , a member of the cytosolic tyrosine kinase family , which is known to mediate signals of several non tyrosine kinase receptors . ^^^ The effect is specific as inferred by the lack of effect of heat inactivated GM CSF or neutralized by specific antibodies and by the finding that interleukin 5 , which utilizes a specific alpha chain and the same beta chain of GM CSFR , does not phosphorylate JAK 2 . ^^^ Furthermore , we show that the amount of JAK 2 physically associated with GM CSFR beta chain is increased after GM CSF stimulation and that GM CSF triggers both beta chain and JAK 2 tyrosine phosphorylation . ^^^ Taken together , these results suggest that biologic activities of GM CSF in vascular endothelium may , in part , be elicited by GM CSFR mediated JAK 2 activation . . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
The receptor for granulocyte / macrophage colony stimulating factor ( GM CSF ) consists of a ligand specific low affinity binding chain ( GM CSFR alpha ) and a second chain that is required for high affinity binding and signal transduction . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
In contrast , introduction and activation of the interleukin 5 receptor ( IL 5R ) or of the granulocyte macrophage colony stimulating factor receptor ( GM CSFR ) results in proliferation only . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
We screened RNA from six patients with JCML for mutations in the GM CSF receptor ( GM CSFR ) coding sequence using RT PCR SSCP . ^^^ Polymorphisms of the GM CSFR alpha and beta chains are common , but pathogenic point mutations of the GM CSFR are infrequent in patients with JCML and are unlikely to be involved in the hypersensitivity to GM CSF demonstrated by progenitor cells . . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
OBJECTIVE : This study was carried out to investigate the expression of alpha GM CSF receptor ( alpha GM CSFr ) messenger RNA and protein in the bronchial mucosa of patients with intrinsic or atopic asthma and of control subjects and to correlate the expression of alpha GM CSFr to the number of EG2+ cells ( eosinophils ) and CD68+ cells ( macrophages ) and pulmonary function . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
The granulocyte macrophage colony stimulating factor receptor ( GM CSFR ) is a potential target for toxin directed therapy , because it is overexpressed on many leukemias and solid tumors and apparently not on stem cells . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
The level of GM CSF receptor ( GM CSFR ) expression was determined by flow cytometry after labelling with specific antibodies for the alpha and beta subunits . ^^^ Although the toxicity to DT GM CSF was specifically mediated by the GM CSFR , no correlation was found between the level of expression of the GM CSFR alpha or beta subunit and the sensitivity for DT GM CSF . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
The human granulocyte macrophage colony stimulating factor receptor ( GM CSFR ) alpha chain RNA is alternatively spliced to yield receptor isoforms . ^^^ Because the GM CSFR beta chain ( beta c ) is shared with the receptors for interleukins 3 and 5 it is possible that the alpha chain confers specificity on the GM CSF response and that the different isoforms might refine this response further . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
To identify and test the functional stoichiometry of those PRLr motifs required for transduction and growth , chimeras consisting of the extracellular domain of either the alpha or beta subunit of human granulocyte macrophage colony stimulating factor ( GM CSF ) receptor ( GM CSFr ) and the intracellular domain of the rat PRLr were synthesized . ^^^ Because the high affinity binding of GM CSF results from the specific pairing of one alpha and one beta GM CSFr , use of GM CSFr / PRLr chimera enabled targeted dimerization of the PRLr intracellular domain . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Granulocyte macrophage colony stimulating factor ( GM CSF ) activity is mediated by a cellular receptor ( GM CSFR ) that is comprised of an alpha chain ( GM CSFRalpha ) , which specifically binds GM CSF , and a beta chain ( betac ) , shared with the interleukin 3 and interleukin 5 receptors . ^^^ Granulocyte macrophage colony stimulating factor ( GM CSF ) activity is mediated by a cellular receptor ( GM CSFR ) that is comprised of an alpha chain ( GM CSFRalpha ) , which specifically binds GM CSF , and a beta chain ( betac ) , shared with the interleukin 3 and interleukin 5 receptors . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
High affinity receptor for IL 5 ( IL 5R ) , a predominant eosinophil maturation factor , is composed of an IL 5 binding alpha chain ( IL 5R alpha ) and a signal transducing beta chain that is shared by IL 3 and granulocyte macrophage CSF ( GM CSF ) receptors ( IL 3R and GM CSFR ) . ^^^ By Northern blot analysis of mRNAs obtained from normal human blood eosinophils , we show in this report that the hematopoietic cytokines IL 5 , IL 3 , and GM CSF down regulate IL 5R alpha mRNA while up regulating alpha chain mRNAs for both IL 3R and GM CSFR as well as the beta chain mRNA . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
GM CSF receptor ( GM CSFR ) and c Fes tyrosine kinase were also activated upon GM CSF stimulation . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
In this regard the AA have evaluated in primary cultures of human gengival normal fibroblasts ( PG 1 cells ) : a ) the expression of GM CSF receptor ( GM CSFR ) ( alfa unit ) on the cell surface ; b ) the in vitro effects of different doses of GM CSF on the GM CSFR expression and on the proliferation and activity of fibroblasts . ^^^ PG 1 cells have been stimulated in vitro with different concentrations of GM CSF ( 10 , 50 , 80 , 100 and 150 ng / ml ) using promonocytic cell line U 937 as positive control for GM CSFR expression . ^^^ At high GM CSF concentration ( 80 ng / ml ) the AA observed : 1 ) A marked increase of GM CSFR expression evaluated as fluorescence intensity ( about three fold in respect to the controls ) ; 2 ) Maximal increase of PG 1 cells proliferation . ^^^ These results demonstrate : 1 ) The presence of GM CSFR on the surface of fibroblasts ; 2 ) The proliferation and the synthesis activity of these cells ( in vitro ) are modulated by different concentration of GM CSF . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Therefore we investigated ( 1 ) the expression levels of the GM CSF receptor ( GM CSFR ) constituents in three malignant plasma cell lines and in native malignant plasma cells , ( 2 ) the ability of the receptor to mediate common signalling pathways regulating proliferation and cell survival in malignant plasma cell lines , and ( 3 ) the effects of GM CSF on tumour cell biology . ^^^ We conclude that ( 1 ) the functional GM CSFR is commonly expressed on malignant plasma cells and that ( 2 ) GM CSF promotes the clonal expansion of myeloma cells by inhibiting spontaneous apoptosis and promoting DNA synthesis . . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Receptors for IL 1beta , IL 6 , and GM CSF were readily detectable in all epithelial cell preparations at levels equal to ( GM CSFR ) or lower than those seen on monocytes . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
We examined GM CSF / GM CSF receptor ( GM CSFR ) gene expression in 20 human non small cell lung cancer ( NSCLC ) xenografts . ^^^ A NSCLC xenograft ( LC 11 JCK ) , expressed GM CSFR but not GM CSF , and showed more rapid growth in GM Tg SCID than non GM CSF transgenic SCID mice ( non Tg SCID ) . ^^^ GM CSF gene expression was detected in 48 of 90 ( 53 % ) primary NSCLC human specimens and GM CSFR gene expression was detected in 42 specimens ( 47 % ) . ^^^ GM CSF expression was detected in 13 of 30 squamous cell carcinoma specimens ( 43 % ) and GM CSFR expression was detected in 10 specimens ( 33 % ) . ^^^ Patients with squamous cell carcinoma coexpressing GM CSF and GM CSFR showed significantly poorer prognosis than those expressing neither GM CSF nor GM CSFR ( P < 0 . 05 , Cox Mantel test ) . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
We describe here expression defects of betac or betac plus GM CSF receptor alpha chain ( GM CSFR alpha ) in 3 pediatric patients with AML and PAP symptoms . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Expression of GM CSF receptor ( GM CSFR ) alpha and beta mRNA subunits by cumulus oocyte complexes and mural granulosa cells was examined using RT PCR . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
The proportion of PCM positive for the GM CSF receptor ( GM CFSR ) alpha chain was examined by flow cytometric analysis and the expression of GM CSFR alpha chain mRNA in PCM was examined by reverse transcription / polymerase chain reaction analysis . ^^^ CONCLUSION : These findings suggest that GM CSF may stimulate GM CSFR alpha chain positive PCM and that these PCM may augment LAK activities developed by PLY and PBL . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
In order to understand a possible inter relationship between the G CSF and GM CSF induced leukocyte responses and expression levels of receptors for G CSF ( G CSFr ) and GM CSF ( GM CSFr ) , the levels of each receptor and CSF were measured in patients with basophilia ( 8 ) , eosinophilia ( 14 ) and bacterial infection showing neutrophilia ( 12 ) in comparison with normal healthy adults ( 12 ) and children ( 14 ) . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Among the downregulated genes in DCs were genes that encode proteins of cell cycle regulation : mitotic growth and transcription activator , platelet derived growth factor receptor beta subunit , interleukin 2 receptor ( IL 2R ) gamma subunit , IL 7R alpha subunit , leucocyte interferon gamma ( IFN gamma ) and granulocyte macrophage colony stimulating factor receptor ( GM CSFR ) . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
As a further comparison of the actions of the two CSFs , CSF 1R and GM CSFR levels were measured by flow cytometry , and it was shown that GM CSFR levels decreased within 5 days of culture , independent of the conditions examined . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Freshly prepared Langerhans cells ( fLC ) expressed the mRNA for receptors for M CSF ( cfms ) , GM CSF ( GM CSFR ) , and TNF alpha ( TNFRII ) . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Furthermore , chimeric receptors consisting of cytosolic and transmembrane part of ovine GHR or ovine PRLR and ECDs of human granulocyte macrophage colony stimulating factor receptor ( GM CSFR ) alpha or beta were constructed . ^^^ Upon transfection into Chinese hamster ovary cells along with reporter luciferase gene and stimulation by GM CSF , a significant increase in luciferase activity occurred when GM CSFR alpha PRLR and GM CSFR beta GHR or GM CSFR alpha GHR and GM CSRR beta PRLR were cotransfected . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Both KA and MT 1 + 2 deficiency significantly affected the expression of MT 3 , granulocyte macrophage colony stimulating factor ( GM CSF ) and its receptor ( GM CSFr ) . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
We analyzed the comparative amounts of granulocyte colony stimulating factor ( G CSFr ) and granulocyte macrophage CSF ( GM CSFr ) receptors expressed on neutrophils and monocytes in measles patients to investigate the role of these CSFrs in the development of leukopenia including neutropenia and monocytopenia in measles . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
The proportion of GM CSFR positive PBE and in vitro responsiveness of PBE to recombinant GM CSF were not different between the groups . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Zidovudine ( AZT ) treatment suppresses granulocyte monocyte colony stimulating factor receptor type alpha ( GM CSFR alpha ) gene expression in murine bone marrow cells . ^^^ In this study , we have observed that AZT exposure also induced a concentration dependent suppressive effect ( 35 90 % ) on GM CSF receptor type alpha ( GM CSFR alpha ) gene expression . ^^^ Exposure to increasing concentrations ( 10 500 U / ml ) of GM CSF was unable to override the suppressive effect of AZT on CFU GM derived colonies , however , treatment in combination with IL 3 ( 10 250 U / ml ) ameliorated the suppressive effects of AZT on CFU GM and on GM CSFR alpha and IL 3R alpha gene expression . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
GM CSF receptor ( GM CSFR ) was down regulated by GM CSF or phorbol 12 myristate 13 acetate in both of the normal controls and SLE patients , while this change was more remarkable in the latter . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate the iufluence of dexamethasone ( DM ) on expression of interleukin 5 receptor alpha ( IL 5R alpha ) , interleukin 3 receptor alpha ( IL 3R alpha ) , granulocyte macrophage colony stimulating factor receptor alpha ( GM CSFR alpha ) and common beta receptor ( beta cR ) in bronchalveolar lavage fluid ( BALF ) eosinophils in guinea pig asthma model , and to study the mechanism of promotion effect of steroid on eosinophil apoptosis . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
In this work we demonstrated the expression of the receptor for GM CSF ( GM CSFR ) on human normal skin fibroblasts from healthy subjects ( NFPC ) and on a human normal fibroblast cell line ( NHDF ) and we try to investigate the biological effects of this cytokine . ^^^ Human normal fibroblasts were cultured with different doses of GM CSF to study the effects of this factor on GM CSFR expression , on cell proliferation and adhesion structures . ^^^ The growth rate of fibroblasts from healthy donors ( NFPC ) is not augmented by GM CSF stimulation in spite of increased expression of the GM CSFR . ^^^ We suggest that the in vitro treatment with GM CSF can shift human normal fibroblasts towards a more differentiated state , due or accompanied by an increased expression of GM CSFR and that such `` differentiation ' ' is an important event induced by such cytokine . . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Cultured fibroblasts obtained from skin biopsies in SSc involved and non involved areas and norrmal skin fibroblasts were cultured with different doses of granulocyte macrophage colony stimulating factor ( GM CSF ) to study the effects of this factor on the expression of GM CSF receptor ( GM CSFR ) on fibroblast proliferation and cellular adhesion structures . ^^^ GM CSF stimulation in vitro did not increase SSc fibroblast growth , in spite of a strongly increased expression of the GM CSFR . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
GM CSF is an important growth factor for cells of both the macrophage and granulocyte lineages and enhances effector functions of these cells via the heterodimeric GM CSF receptor ( GM CSFR ) . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
GM CSF receptor ( GM CSFR ) expression was determined by Western blot of cell lysates . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
Granulocyte macrophage colony stimulating factor ( GM CSF ) up regulates HLA DR expression on monocytes via the GM CSF receptor ( GM CSFr ) through a transcriptional mechanism involving the class 2 transactivator factor ( CIITA ) . ^^^ Granulocyte macrophage colony stimulating factor ( GM CSF ) up regulates HLA DR expression on monocytes via the GM CSF receptor ( GM CSFr ) through a transcriptional mechanism involving the class 2 transactivator factor ( CIITA ) . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
The Granulocyte Macrophage Colony Stimulating Factor ( GM CSF ) is a hematopoietic growth factor that regulates the in vitro and in vivo proliferation and differentiation of hematopoietic cells through the interaction with a specific heterodimeric receptor complex ( GM CSFR ) , consisting of an alpha and a beta chain with molecular weights of 80 and 120 KDa , respectively . ^^^ We have studied the expression of the GM CSFR ( alpha chain ) on the surface of the human osteosarcoma cell line SaOS 2 and the in vitro effects of different concentrations ( 10 , 100 , and 200 ng / ml ) of GM CSF on GM CSFR expression and the biological activity of SaOS 2 cells . ^^^ Our data show that SaOS 2 cells express GM CSFR and that GM CSF can down regulate the expression of its own receptor on these cells . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
A number of residues located on the B and C helices of GM CSF are postulated to interact with the alpha chain of the GM CSF receptor ( GM CSFR ) . ^^^ Key residues involved in CDR 1 peptide / GM CSFR binding were identified by truncation and alteration of individual residues , while the structural elements required to antagonize the biological action of GM CSF were separately tested in binding and inhibitory activity assays of multiple cyclic analogues . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
By transient transfection experiments with HIV 1 LTR reporter constructs , we showed that strong LTR mediated activation was induced by GM CSF in mouse Ba / F3 cells expressing human GM CSF receptors ( GM CSFR ) . ^^^ Further analyses of GM CSFR mutants suggested that some other unknown signalling pathway also participates in GM CSF induced HIV 1 LTR activation . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
These data suggest that a majority of CLPs and a fraction of pro T cells possess plasticity for myelomonocytic differentiation that can be activated by ectopic GM CSF signals , supporting the hypothesis that the down regulation of GM CSFR is a critical event in producing cells with a lymphoid restricted lineage potential . . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
We have now extended this concept by using extracellular domains of the heteromeric granulocyte / macrophage colony stimulating factor receptor ( GM CSFR ) . ^^^
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
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Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA
Interacting proteins: P15509 and P04141	Pubmed	SVM Score :0.0
NA