| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| Thus , in response to 5 src expression , a Ras / MEKK 1 / JNK signal transduction pathway activating c Jun and a Ras / Raf 1 / ERK pathway converge to mediate pgs 2 gene expression via the CRE site in the pgs 2 promoter . . ^^^ |
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| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| MEK 5 is thought to lie in an uncharacterized MAP kinase pathway , because MEK 5 does not phosphorylate the ERK / MAP kinase family members ERK 1 , ERK 2 , ERK 3 , JNK / SAPK , or p38 / HOG1 , nor will Raf 1 , c Mos , or MEKK 1 highly phosphorylate it . ^^^ |
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| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| Mono Q fractionation of LIF stimulated 3T3 L 1 extracts identified a single peak of MAPKKK activity that was largely insensitive to elevated intracellular levels of cAMP , and that failed to correlate with stimulation of either Raf 1 or MEKK 1 protein kinases . ^^^ |
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| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| Taken together , these findings indicate that PKC alpha and PKC epsilon can enhance the activities of at least three signaling pathways that converge on the SRE : c Raf MEK 1 ERK TCF , MEKK 1 SEK1 JNK TCF , and rhoA SRF . ^^^ |
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| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| Although JSAP 1 coprecipitated with MEK 1 MAPKK and Raf 1 MAPKKK , and not MKK 6 or MKK 7 MAPKK , in cotransfected COS 7 cells , MEK 1 and Raf 1 do not interfere with the binding of SEK 1 and MEKK 1 to JSAP 1 , respectively . ^^^ |
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| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| Western immunoblotting confirmed that Raf 1 , A Raf , B Raf , MEKK 1 and MEKK 2 were present at similar levels in E 19 and adult liver . ^^^ |
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| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| Interestingly , the counterpart of Raf 1 in the c Jun N terminal kinase ( JNK ) cascade , MEKK 1 ( mitogen activated protein kinase / ERK kinase ) , is similarly associated with Hsp 90 , and this association as well as the activation of MEKK 1 are disrupted by dexamethasone treatment . ^^^ Disruption of the ERK and JNK cascades at the level of Raf 1 and MEKK 1 could account for the inhibitory action of dexamethasone on the generation of inflammatory mediators in stimulated mast cells . . ^^^ |
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| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| Irreversible commitment to apoptosis in this cell model may derive from loss of Raf 1 and cleavage / activation of MEKK 1 , processes reported in other models to be caspase mediated . ^^^ |
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| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| MEKK 1 binds raf 1 and the ERK 2 cascade components . ^^^ In this study we demonstrate that endogenous MEKK 1 binds to endogenous ERK 2 , MEK 1 , and another MEKK level kinase , Raf 1 , suggesting that it can assemble all three proteins of the ERK 2 MAP kinase module . . ^^^ |
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| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| Further analyses disclosed that transfection of dominant negative forms of raf 1 , MEK 1 , ERK 1 , ERK 2 , or wild type MEKK 1 all increased cystatin A promoter activity in normal human keratinocytes , whereas wild type raf 1 , ERK 1 , ERK 2 , or dominant negative forms of MEKK 1 , MKK 7 , or JNK 1 suppressed the promoter activity . ^^^ |
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| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| Co transfection of the MAO B promoter with dominant negative forms of Ras , Raf 1 , MEKK 1 , MEK 1 , MEK 3 , MEK 7 , ERK 2 , JNK 1 , and p38 / RK inhibit the PMA dependent activation of the MAO B promoter . ^^^ |
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| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| Transfection of human embryonic 293 cells with a kinase defective Raf 1 or a kinase defective mitogen activated protein kinase / extracellular signal regulated kinase kinase 1 ( MEKK 1 ) suppressed Bcl 2 mediated IKK beta activity and NF kappa B activation . ^^^ In this report , we provide the first direct evidence that Bcl 2 activates NF kappa B by a signaling mechanism that involves Raf 1 / MEKK 1 mediated activation of IKK beta . . ^^^ |
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| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| We expressed the catalytic fragment of human MEKK 1 to activate JNK and the Ras binding domain ( RBD ) of Raf 1 to inhibit the Ras ERK pathway . ^^^ Using mutant forms of Ras and Raf 1 we could show that the reduced kinase activity of RBD MEKK 1 fusion proteins was caused by binding to the Ras protein . ^^^ |
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| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| These results suggested that the alteration of intracellular milieu induced by MG through a MEKK 1 mediated and PI3K / PKC / Raf 1 independent pathway resulted in the modification of cell response to IGF 1 for p 21 ( Waf1 / Cip1 ) mediated growth arrest , which may be one of the crucial mechanisms for MG to promote the development of chronic clinical complications in diabetes . . ^^^ |
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| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| Prx 1 gene induction by TPA was decreased by protein kinase C inhibitors and overexpressed dominant negative forms of Ras and MEKK 1 , but not Raf 1 . ^^^ |
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| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| The DVD sites bind to their specific upstream MAP kinase kinase kinases ( MAPKKKs ) , including MTK 1 ( MEKK 4 ) , ASK 1 , TAK 1 , TAO 2 , MEKK 1 , and Raf 1 . ^^^ |
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| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| TAK 1 ( transforming growth factor beta activated kinase 1 ) , a MAP3K with considerable sequence similarity to Raf 1 and MEKK 1 , has been identified as a transforming growth factor beta / bone morphogenetic protein ( BMP ) activated cytosolic component of the MAPK pathways . ^^^ |
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| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| Fractions containing kinase activity were also probed with antibodies against MEK kinase 1 , Raf 1 , A Raf , B Raf , Mos , and Tpl 2 . ^^^ |
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| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| Raf 1 kinase ( Raf 1 ) and ( MAPK / extracellular signal regulated kinase ) kinase kinase ( MEKK ) , both of which have MAPKKK activity , were also activated by stretching cardiac myocytes for 2 min . ^^^ These results suggest that mechanical stress activates the protein kinase cascade of phosphorylation in cardiac myocytes in the order of Raf 1 and MEKK , MAPKK , MAPKs and p90rsk , and that angiotensin 2 , which may be secreted from stretched myocytes , may be partly involved in stretch induced hypertrophic responses by activating PKC . . ^^^ |
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| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| Furthermore , Raf 1 and MEKK were activated strikingly by the osmotic shock . ^^^ Activation of Raf 1 and MEKK in response to osmotic shock was detected also in PC 12 cells , in which MEKK activation by the osmotic shock was much stronger than that by epidermal growth factor . ^^^ These results suggest that the MAPK cascade consisting of Raf 1 , MAPKK , and MAPK and the SAPK cascade consisting of MEKK , XMEK 2 , and SAPK are both activated in response to osmotic shock . ^^^ |
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| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| These observations , together with a recent finding that several MAPKK Ks such as Raf 1 and MEKK may function within a cell , suggest a diversity of MAPKK Ks . ^^^ |
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| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| Differential activation of ERK and JNK mitogen activated protein kinases by Raf 1 and MEKK . ^^^ Ras activates two protein kinases , Raf 1 and MEK ( MAPK , or ERK , kinase ) kinase ( MEKK ) . ^^^ Raf 1 contributes directly to ERK activation but not to JNK activation , whereas MEKK participated in JNK activation but caused ERK activation only after overexpression . ^^^ These results demonstrate the existence of two distinct Ras dependent MAPK cascades one initiated by Raf 1 leading to ERK activation , and the other initiated by MEKK leading to JNK activation . . ^^^ |
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| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| D . , n = 3 ) . 1 MEKK was characterized by anion exchange and gel filtration chromatography and separated into two distinct activities of approximately 56 kDa that phosphorylated and activated MEK . 1 MEKKs did not co elute on anion exchange with c Raf or 73 kDa MEK kinase ( MEKK ) , suggesting they are distinct enzymes . ^^^ |
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| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| Three MAP kinase kinase kinases have been described c Raf , c Mos , and Mekk that phosphorylate and activate Mek , the MAP kinase kinase . ^^^ |
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| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| We have identified a protein kinase , MUK , as an activator of the JNK pathway , whose kinase domain shows significant homology to MAPKKK related proteins such as c Raf and MEKK . ^^^ |
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| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| Receptor induced activation of other Ser / Thr kinases , such as c Raf , B Raf , Mek 1 , Mek 2 , Mekk , was not affected by wortmannin . ^^^ |
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| Interacting proteins: Q13233 and P04049 |
Pubmed |
SVM Score :0.0 |
| While activation of tyrosine kinase growth factor receptors is accompanied by hyperphosphorylation of Raf 1 , stimulation of receptors coupled to G proteins has in some cases been shown to result in activation of a non Raf MEKK rather than of Raf itself . ^^^ |
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