| Interacting proteins: Q13188 and P04049 |
Pubmed |
SVM Score :0.0 |
| Role of the kinase MST 2 in suppression of apoptosis by the proto oncogene product Raf 1 . ^^^ We used proteomic analysis of Raf 1 signaling complexes to show that Raf 1 counteracts apoptosis by suppressing the activation of mammalian sterile 20 like kinase ( MST 2 ) . ^^^ Raf 1 prevents dimerization and phosphorylation of the activation loop of MST 2 independently of its protein kinase activity . ^^^ Depletion of MST 2 from Raf 1 / mouse or human cells abrogated sensitivity to apoptosis , whereas overexpression of MST 2 induced apoptosis . ^^^ Conversely , depletion of Raf 1 from Raf 1+ / + mouse or human cells led to MST 2 activation and apoptosis . ^^^ |
|
| Interacting proteins: Q13188 and P04049 |
Pubmed |
SVM Score :0.0 |
| Raf 1 inhibits MST 2 by preventing its dimerisation and recruiting a phosphatase that removes activating phosphorylations on MST 2 . ^^^ Both functions require Raf 1 binding to MST 2 , but are independent of Raf 1 ' s kinase activity and the ERK pathway . ^^^ Downregulation of MST 2 by siRNA reverts the apoptosis hypersensitivity of Raf 1 ( / ) mouse fibroblasts . ^^^ In contrast , the downregulation of Raf 1 in Raf 1 ( + / + ) cells and human cancer cell lines enhances susceptibility to Fas induced apoptosis , which is rescued by concomitant downregulation of both Raf 1 and MST 2 . ^^^ The MST 2 : Raf 1 complex is dissociated by stress signals as well as mitogens . ^^^ |
|
| Interacting proteins: Q13188 and P04049 |
Pubmed |
SVM Score :0.0 |
| Recently , we showed that Raf 1 kinase sequesters and inhibits MST 2 and that this event is critical for Raf mediated cell survival . ^^^ |
|