| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.69528369 |
| Although direct connections of Rap 1 with the serine / threonine kinases Raf 1 and B raf have been reported , we were unable to find functional evidence for an interaction of endogenous Rap 1 signalling with the Raf / extracellular signal regulated kinase ( ERK ) pathway . 0.69528369^^^ |
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| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| Phosphorylation of RGL did not affect its binding to ras p 21 and rap 1 p21 under the conditions that phosphorylation of Raf 1 reduced its affinity for ras p 21 . ^^^ These results demonstrate that rap 1 p21 but not protein kinase A regulates the interaction of ras p 21 with RGL and suggest that rap 1 p21 and protein kinase A may cooperate to distinguish the signal or ras p 21 to RGL from that to Raf 1 . . ^^^ |
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| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| Rap 1 , activated by mutation or by the cAMP dependent protein kinase PKA , is a selective activator of B Raf and an inhibitor of Raf 1 . ^^^ |
|
| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| The inactivation of Rap 1 was associated with an insulin stimulated decrease in the amount of Rap 1 that was bound to Raf 1 . ^^^ In parallel with the dissociation of Raf 1 from Rap 1 , there was an increased association of Raf 1 with Ras . ^^^ Together , these data demonstrate that insulin treatment results in the de repression of Rap 1 inhibitory function on the Raf 1 kinase concomitant with Ras activation and stimulation of the downstream Raf1 / MEK / ERK cascade . . ^^^ |
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| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| In its active GTP bound form , the Rap 1 GTPase may act as a negative regulator of Ras mediated signaling by sequestering Ras effectors ( e . g . , Raf 1 ) and preventing their activation . ^^^ |
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| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| Interestingly , beta ( 2 ) AR activates both the small G proteins Rap 1 and Ras , but only Rap 1 is capable of coupling to Raf isoforms . beta ( 2 ) AR inhibits the Ras dependent activation of both Raf isoforms Raf 1 and B Raf , whereas Rap 1 activation by isoproterenol recruits and activates B Raf . beta ( 2 ) AR activation of ERKs is not blocked by expression of RasN 17 , an interfering mutant of Ras , but is blocked by expression of either RapN 17 or Rap1GAP1 , both of which interfere with Rap 1 signaling . ^^^ |
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| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| Cyclic AMP can either activate or inhibit the mitogen activated protein kinase ( MAPK ) pathway in different cell types ; MAPK activation has been observed in B Raf expressing cells and has been attributed to Rap 1 activation with subsequent B Raf activation , whereas MAPK inhibition has been observed in cells lacking B Raf and has been attributed to cAMP dependent protein kinase ( protein kinase A ) mediated phosphorylation and inhibition of Raf 1 kinase . ^^^ |
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| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| SrcY529F also inhibited activated Ras induced cell detachment but failed to rescue detachment caused by an activated mutant of Raf 1 ( Raf BXB ) that Rap 1 can not inhibit . ^^^ |
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| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| Antibodies directed against either the cAMP activated guanine nucleotide exchange factor Epac 1 , the monomeric G protein Rap 1 or the kinase Raf B , curtailed the stimulation of H , K ATPase by calcitonin , whereas antibodies against the related monomeric G protein Ras or kinase Raf 1 had no effect . ^^^ |
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| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| Raf 1 is also thought to be recruited to the small G protein Rap 1 upon GTP loading of Rap 1 . ^^^ We propose that this is because Raf 1 is not phosphorylated on Tyr 341 upon recruitment to Rap 1 . ^^^ Redirecting Rap 1 to Ras containing membranes or mimicking Tyr 341 phosphorylation of Raf 1 by mutation converts Rap 1 into an activator of Raf 1 . ^^^ In contrast to Raf 1 , B Raf is activated by Rap 1 . ^^^ |
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| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| METHODS : Kidneys of 8 week old normal Han : SPRD rats ( + / + ) or rats heterozygous ( Cy / + ) for ADPKD were examined by Western blot analysis and immunohistochemistry to determine the expression of extracellular regulated kinase ( ERK ) , phosphorylated ERK ( P ERK ) , Raf 1 ( MAPKKK ) , phosphorylated Raf 1 ( P Raf 1 ) , B Raf , Rap 1 and phosphorylated protein kinase A ( P PKA ) . ^^^ |
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| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| In addition , using dominant negative mutants , we show that sst 2 mediated ERK 2 stimulation is dependent on the pertussis toxin sensitive Gi / o protein , the tyrosine kinase Src , both small G proteins Ras and Rap 1 , and the MEK kinase B Raf but is independent of Raf 1 . ^^^ |
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| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| These actions of Rap 1 occur in selected cell types to enhance or diminish ERK signaling , depending on the expression pattern of the MAP kinase kinase kinases of the Raf family : Raf 1 and B Raf . ^^^ |
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| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| A decrease in the phosphorylation of c Raf , MEK1 / 2 , and ERK1 / 2 was accompanied by an increase in Rap 1 expression in both FK 228 treated and Rap 1 overexpressing cells . ^^^ |
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| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE / DESIGN / PATIENTS : In an attempt to clarify such controversies and to find whether or not FVPTC cases share the molecular features of follicular tumors , we searched for the presence of PAX 8 PPARgamma rearrangements , RAS mutations , and RAP 1 , RAF 1 , and BRAF mutations in a series of 40 FVPTCs as well as in 27 follicular thyroid carcinomas ( FTCs ) and 12 follicular thyroid adenomas ( FTAs ) . ^^^ No mutations were detected in RAP 1 and RAF 1 . ^^^ |
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| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| Raf 1 interferes with Ras and Rap1A effector functions in yeast . ^^^ Raf 1 also blocked Rap1A effector function in S . cerevisiae . ^^^ Raf 1 , therefore , appears to interact with H Ras and Rap1A in these in vivo systems with properties that suggest it is an immediate downstream effector . . ^^^ |
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| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| The interactions of H Ras , R Ras , and Rap1A with the Ras binding domains ( RBD ) of the c Raf kinase and of the Ral guanine nucleotide exchange factor ( RGF ) was studied biochemically in solution . ^^^ |
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| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| Coassociation of Rap1A and Ha Ras with Raf 1 N terminal region interferes with ras dependent activation of Raf 1 . ^^^ However , Rap1A , which has an identical effector domain to that of Ras , can not activate Raf 1 and even antagonizes several Ras functions in vivo . ^^^ Both Rap1A and Ha Ras ( E31K ) mutant failed to activate Raf 1 and interfered with Ha Ras dependent activation of Raf 1 in Sf 9 cells . ^^^ Enhanced binding of Rap1A to CRR led to co association of Rap1A and Ha Ras with Raf 1 N terminal region through binding to CRR and Ras binding domain , respectively . ^^^ These results suggest that Rap1A interferes with Ras dependent Raf 1 activation by inhibiting binding of Ras to Raf 1 CRR . . ^^^ |
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| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| Surprisingly , as opposed to Rap1A , which has no effect on Raf 1 oncogene mediated transformation , C3G also reduced dramatically ( 6 8 fold ) the number of 5 raf induced foci in transfected NIH3T3 cells . ^^^ |
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| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| Antibodies against the monomeric G protein Ras or the kinase Raf 1 curtailed the stimulation of H K ATPase by isoproterenol , whereas antibodies against the related proteins Rap 1 and B Raf had no effect . ^^^ |
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| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| The Ras homologue Rap1A is incapable of activating Raf 1 and even antagonizes Ras dependent activation of Raf 1 . ^^^ We proposed previously that this property of Rap1A may be attributable to its greatly enhanced interaction with Raf 1 CRD compared to Ras . ^^^ When interactions with Ras and Rap1A were measured , B Raf CRD did not exhibit the enhanced interaction with Rap1A , suggesting that the strength of interaction at CRDs may account for the differential action of Rap1A on Raf 1 and B Raf . ^^^ The importance of the interaction at the CRD is further supported by a domain shuffling experiment between Raf 1 and B Raf , which clearly indicated that the nature of CRD determines the specificity of response to Rap1A : Raf 1 , whose CRD is replaced by B Raf CRD , became activatable by Rap1A , whereas B Raf , whose CRD is replaced by Raf 1 CRD , lost its response to Rap1A . ^^^ |
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| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| Correspondingly , RasG12V ( E31K ) as well as Rap1A , but not RasG12V ( E31R ) , exhibited abnormally tight binding with the cysteine rich domain of Raf 1 . ^^^ All of these results clearly show that the sharp contrast between the characteristics of Ras and Rap1A , with respect to the interactions with Raf 1 and RalGDS , depends on their residues at position 31 . . ^^^ |
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| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| This is done by taking the complex between Rap1A ( Rap ) and the p21ras binding domain of c Raf ( Raf RBD ) ( Nassar et al . , Nature 375 : 554 560 , 1995 ) as a model system . ^^^ |
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| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| Effect of phosphorylation on activities of Rap1A to interact with Raf 1 and to suppress Ras dependent Raf 1 activation . ^^^ However , it is not known whether Rap1A phosphorylation is involved in regulation of its cellular functions , including suppression of Ras dependent Raf 1 activation . ^^^ We have previously shown that this suppressive activity of Rap1A is attributable to its greatly enhanced ability to bind to the cysteine rich region ( CRR , residues 152 184 ) of Raf 1 compared with that of Ras . ^^^ Furthermore , a mutant Rap1A ( S180E ) , whose sole PKA phosphorylation residue , Ser 180 , was substituted by an acidic residue , Glu , to mimic its phosphorylated form , failed to suppress Ras dependent Raf 1 activation in COS 7 cells . ^^^ These results indicate that the CRR binding activity and the Ras suppressive function of Rap1A can be modulated through phosphorylation and suggest that Rap1A may function as a PKA dependent regulator of Raf 1 activation , not merely as a suppressor . . ^^^ |
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| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| In the complex of the `` Ras like ' ' E30D / K31E mutant Rap1A with the Ras binding domain ( RBD ) , residues 51 131 of Raf 1 , Glu 31 in Rap1A forms a tight salt bridge with Lys 84 in Raf 1 . ^^^ Here , the `` Rap1A like ' ' D30E / E31K mutant of Ras was prepared and shown to bind the Raf 1 RBD less strongly than wild type Ras , but slightly more tightly than the E31K mutant . ^^^ |
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| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| Here , the methods of continuum electrostatics are used to investigate the contribution of electrostatic interactions to the binding of four protein protein complexes ; barnase barstar , human growth hormone and its receptor , subtype N 9 influenza virus neuraminidase and the NC 41 antibody , the Ras binding domain ( RBD ) of kinase cRaf and a Ras homologue Rap1A . ^^^ |
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| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P62834 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|