| Interacting proteins: P30086 and P04049 |
Pubmed |
SVM Score :0.80959915 |
| In vitro , RKIP binds to Raf 1 , MEK and ERK , but not to Ras . 0.80959915^^^ |
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| Interacting proteins: P30086 and P04049 |
Pubmed |
SVM Score :0.7306608 |
| RKIP can form ternary complexes with Raf 1 , MEK , and ERK . 0.7306608^^^ |
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| Interacting proteins: P30086 and P04049 |
Pubmed |
SVM Score :0.76936627 |
| This mode of action bears striking similarities to the interactions of RKIP with Raf 1 and MEK 1 in the MAPK pathway . 0.76936627^^^ |
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| Interacting proteins: P30086 and P04049 |
Pubmed |
SVM Score :1.6510725 |
| RKIP has been shown to disrupt the Raf 1 MEK1 / 2 [ mitogen activated protein kinase ERK ( extracellular signal regulated kinase ) kinase 1 / 2 ] ERK1 / 2 and NF kappaB signaling pathways , via physical interaction with Raf 1 MEK1 / 2 and NF kappaB inducing kinase or transforming growth factor beta activated kinase 1 , respectively , thereby abrogating the survival and antiapoptotic properties of these signaling pathways . 1.6510725^^^ |
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| Interacting proteins: P30086 and P04049 |
Pubmed |
SVM Score :0.56043424 |
| Rituximab treatment of NHL B cells significantly up regulated RKIP expression , thus interrupting the ERK1 / 2 signaling pathway through the physical association between Raf 1 and RKIP , which was concomitant with Bcl ( xL ) down regulation . 0.56043424^^^ |
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| Interacting proteins: P30086 and P04049 |
Pubmed |
SVM Score :0.51099558 |
| HCNP precursor protein ( HCNP pp ) is known to interact with other molecules including phosphatidylethanolamine and Raf 1 kinase , and is also known as phosphatidylethanolamine binding protein and raf kinase inhibitory protein . 0.51099558^^^ |
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| Interacting proteins: P30086 and P04049 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : Raf 1 kinase inhibitory protein ( RKIP ) was recently identified as a physiologic endogenous inhibitor of the extracellular signal regulated kinase ( ERK ) pathway . ^^^ Studies using asRKIP and ssRKIP demonstrated that RKIP blocked activation of MEK and ERK by Raf 1 in beta cells . ^^^ |
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| Interacting proteins: P30086 and P04049 |
Pubmed |
SVM Score :0.0 |
| We have previously identified Raf kinase inhibitor protein ( RKIP ) as a negative regulator of the Raf MEK ERK signaling pathway by specifically binding to the Raf 1 isoform . ^^^ |
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| Interacting proteins: P30086 and P04049 |
Pubmed |
SVM Score :0.0 |
| The rat homologue ( known as RKIP ; Raf 1 kinase inhibitor protein ) has been shown to negatively regulate the MAP kinase pathway through formation of inhibitory complexes with Raf 1 and MEK . ^^^ |
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| Interacting proteins: P30086 and P04049 |
Pubmed |
SVM Score :0.0 |
| Here we report that one mechanism involves dissociation of Raf kinase inhibitory protein ( RKIP ) from Raf 1 . ^^^ RKIP Ser 153 phosphorylation by PKC either in vitro or in response to 12 O tetradecanoylphorbol 13 acetate or epidermal growth factor causes release of RKIP from Raf 1 , whereas mutant RKIP ( S153V or S153E ) remains bound . ^^^ |
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| Interacting proteins: P30086 and P04049 |
Pubmed |
SVM Score :0.0 |
| After stimulation of GPCR , RKIP dissociates from its known target , Raf 1 ( refs 6 8 ) , to associate with GRK 2 and block its activity . ^^^ |
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| Interacting proteins: P30086 and P04049 |
Pubmed |
SVM Score :0.0 |
| In this study , we provide evidence that the Raf 1 kinase inhibitory protein ( RKIP ) and its effects on Raf 1 mediated activation of mitogen activated protein / extracellular signal regulated kinase kinase are important for the metastatic potential of malignant melanoma . ^^^ |
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| Interacting proteins: P30086 and P04049 |
Pubmed |
SVM Score :0.0 |
| RKIP binds inhibits Raf 1 mediated phosphorylation of MEK through binding to Raf 1 . ^^^ Protein kinase C ( PKC ) phosphorylates RKIP , resulting in release of Raf 1 and activation of MEK and ERK . ^^^ |
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| Interacting proteins: P30086 and P04049 |
Pubmed |
SVM Score :0.0 |
| RKIP is also the first MAP kinase signaling modulator to be identified as playing a role in cancer metastasis , and identification of the mechanism by which it regulates Raf 1 activation provides new targets for therapeutic intervention . . ^^^ |
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| Interacting proteins: P30086 and P04049 |
Pubmed |
SVM Score :0.0 |
| Rituximab upregulates Raf 1 kinase inhibitor protein ( RKIP ) expression in non ARL cells . ^^^ Through physical association with Raf 1 and nuclear factor kappaB ( NF kappa B ) inducing kinase ( NIK ) , RKIP negatively regulates two major survival pathways , namely , the extracellular signal regulated kinase1 / 2 ( ERK1 / 2 ) and the NF kappa B pathways , respectively . ^^^ |
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| Interacting proteins: P30086 and P04049 |
Pubmed |
SVM Score :0.0 |
| Here , we show that RKIP inhibits MAPK by regulating Raf 1 activation ; specifically , RKIP acts subsequent to Raf 1 membrane recruitment , prevents association of Raf 1 and p 21 activated kinase ( PAK ) , and blocks phosphorylation of the Raf 1 kinase domain by PAK and Src family kinases . ^^^ Mutation of the PAK and Src phosphorylation sites on Raf 1 to aspartate , a phosphate mimic , prevented RKIP association with or inhibition of Raf 1 signaling . ^^^ Because c Raf 1 and B Raf are both required for maximal MAPK stimulation by epidermal growth factor in neuronal and epithelial cell lines , we determined whether RKIP significantly affects MAPK signaling . ^^^ These results indicate that selective modulation of c Raf 1 but not B Raf activation by RKIP can limit the dynamic range of the MAPK signaling response to growth factors and may play a critical role in growth and development . . ^^^ |
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| Interacting proteins: P30086 and P04049 |
Pubmed |
SVM Score :0.0 |
| The target of locostatin , a small molecule inhibitor of cell movement , has been identified as RKIP , a Raf 1 kinase modulator [ 1 ] . ^^^ |
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| Interacting proteins: P30086 and P04049 |
Pubmed |
SVM Score :0.0 |
| Locostatin abrogates RKIP ' s ability to bind and inhibit Raf 1 kinase , and it acts by disrupting a protein protein interaction , an uncommon mode of action for a small molecule . ^^^ |
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| Interacting proteins: P30086 and P04049 |
Pubmed |
SVM Score :0.0 |
| PURPOSE : Raf 1 kinase inhibitor protein ( RKIP ) was originally identified as the first physiologic inhibitor of the Raf / mitogen activated protein kinase kinase / extracellular signal regulated kinase ( ERK ) pathway . ^^^ |
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| Interacting proteins: P30086 and P04049 |
Pubmed |
SVM Score :0.0 |
| We also demonstrate that phosphatidylethanolamine binding protein ( alternatively named Raf 1 kinase inhibitor protein or RKIP ) acts as an endogenous morphine 6 glucuronide binding protein . ^^^ |
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| Interacting proteins: P30086 and P04049 |
Pubmed |
SVM Score :0.0 |
| We analyzed expression of Raf 1 kinase inhibitory protein ( RKIP ) in HCC . ^^^ |
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| Interacting proteins: P30086 and P04049 |
Pubmed |
SVM Score :0.0 |
| Raf 1 depletion or MEK inhibition reverses the reduction in the mitotic index , whereas hyperactivation of Raf mimics the RKIP depletion phenotype . ^^^ These results indicate that RKIP regulates Aurora B kinase and the spindle checkpoint via the Raf 1 / MEK / ERK cascade and demonstrate that small changes in the MAP kinase ( MAPK ) pathway can profoundly impact the fidelity of the cell cycle . . ^^^ |
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| Interacting proteins: P30086 and P04049 |
Pubmed |
SVM Score :0.0 |
| Hippocampal cholinergic neurostimulating peptide precursor protein ( HCNP pp ) is a unique multifunctional protein , being not only the precursor of HCNP , which promotes the phenotype development of septo hippocampal cholinergic neurons , but also the binding protein of phosphatidylethanolamine , ATP , Raf 1 kinase ( known as `` Raf 1 kinase inhibitory factor ' ' in peripheral organs ) , and serine protease . ^^^ |
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| Interacting proteins: P30086 and P04049 |
Pubmed |
SVM Score :0.0 |
| We were able to identify a number of proteins , which include Rho guanine nucleotide dissociation inhibitor 1 , phosphatidylethanolamine binding protein ( PEBP ) , also termed Raf 1 kinase interacting protein , polyubiquitin , immunophilin FK 506 binding protein 12 ( FKBP 12 ) and cystatin C . ^^^ |
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| Interacting proteins: P30086 and P04049 |
Pubmed |
SVM Score :0.0 |
| Phosphatidylethanolamine binding protein ( PEBP ) has been demonstrated to bind to Raf 1 and mitogen activated protein kinase kinase , components of the extracellular signal regulated protein kinase ( ERK ) pathway , thereby inhibiting the pathway and resulting in the suppression of cell proliferation . ^^^ |
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