| Interacting proteins: P23458 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23458 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23458 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P23458 and P04049 |
Pubmed |
SVM Score :0.0 |
| Therefore , JAK 1 activity may be involved in activation of Raf 1 and ERK 1 via G proteins activated by C5b 9 . ^^^ |
|
| Interacting proteins: P23458 and P04049 |
Pubmed |
SVM Score :0.0 |
| Beta interferon and oncostatin M activate Raf 1 and mitogen activated protein kinase through a JAK 1 dependent pathway . ^^^ IFN beta and OSM induced Raf 1 activity could be coimmunoprecipitated with either Jak 1 or Tyk 2 . ^^^ Furthermore , HeLa cells lacking Jak 1 displayed no activation of STAT1alpha , STAT 3 , and Raf 1 by IFN beta or OSM and also demonstrated no increase in the relative level of GTP bound p21ras in response to OSM . ^^^ The requirement for Jak 1 for IFN beta and OSM induced activation of Raf 1 was also seen in Jak 1 deficient U4A fibrosarcoma cells . ^^^ Interestingly , basal MAPK , but not Raf 1 , activity was constitutively enhanced in Jak 1 deficient HeLa cells . ^^^ |
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| Interacting proteins: P23458 and P04049 |
Pubmed |
SVM Score :0.0 |
| Interferon gamma activation of Raf 1 is Jak 1 dependent and p21ras independent . ^^^ In HeLa ( E1C3 ) and fibrosarcoma ( U4A ) cell lines , both of which are deficient in Jak 1 kinase , Raf 1 activation by IFNgamma was absent . ^^^ Reconstitution of Raf 1 activity was observed only with kinase active Jak 1 in both cell lines . ^^^ In COS cells , transient expression of wild type or kinase inactive Jak 1 coimmunoprecipitated with Raf 1 , but activation of Raf 1 activity was only observed in cells expressing kinase active Jak 1 . ^^^ These observations suggest that a kinase active Jak 1 is required for IFNgamma activation of Raf 1 that is p 21 ( ras ) independent . . ^^^ |
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