| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :1.0877081 |
| Moreover , Raf 1 directly competes with Rin 1 for binding to H Ras in vitro . 1.0877081^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.57569811 |
| GTP dependent association of Raf 1 with Ha Ras : identification of Raf as a target downstream of Ras in mammalian cells . 0.57569811^^^ The GTP dependent association of Ha Ras with Raf 1 was observed with lysates of various types of cultured cells , including NIH 3T3 , pheochromocytoma ( PC ) 12 , Ba / F3 , and Jurkat T cells , and also with crude extracts from rat brain . 0.51185458^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.92207401 |
| Here , we found that Raf 1 CRR interacts directly with Ha Ras independently of RBD and that participation of CRR is necessary for efficient Ras Raf binding . 0.92207401^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.72679533 |
| To prove possible differences in their recognition mechanisms of Ras , we screened 44 human Ha Ras proteins carrying mutations in the effector region and its flanking sequences for interaction with human Raf 1 , Schizosaccharomyces pombe Byr 2 , and Saccharomyces cerevisiae adenylyl cyclase . 0.72679533^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.99746969 |
| As an association of Raf 1 with p21Ras does not activate Raf 1 kinase , but merely targets Raf 1 to the plasma membrane , we examined the binding of Raf 1 to 14 3 3 proteins and to the insulin receptor itself . 0.99746969^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.54081669 |
| Contraction also resulted in an apparent increase in the association of Raf 1 with p21Ras , although stimulation of MAP kinase signaling occurred independent of Shc , IRS 1 , and IRS 2 tyrosine phosphorylation or the formation of Shc / Grb2 or IRS1 / Grb2 complexes . 0.54081669^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.84638229 |
| We have examined the interaction between Raf 1 and human H ras in three different systems that utilize H ras induced phenotypes in Saccharomyces cerevisiae . 0.84638229^^^ Raf 1 , therefore , appears to interact with H Ras and Rap1A in these in vivo systems with properties that suggest it is an immediate downstream effector . . 0.79733428^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.78934115 |
| B Raf was shown to share almost the same H Ras binding specificity with Raf 1 by examining binding of the H Ras mutants to Raf 1 and B Raf in the yeast two hybrid and in vitro binding assays . 0.78934115^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.7216317 |
| Recent studies have shown that elevated expression of the rate limiting R 2 component of ribonucleotide reductase increases Raf 1 protein activation and mitogen activated protein kinase activity and acts as a novel malignancy determinant in cooperation with activated oncogenes like H ras . 0.7216317^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :1.0442176 |
| Recent studies have shown that elevated expression of the rate limiting R 2 component increases Raf 1 protein activation and mitogen activated protein kinase activity and acts as a novel malignancy determinant in cooperation with H ras and rac 1 . 1.0442176^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.96639262 |
| Analysis of the Ras signalling complexes in immunoprecipitates from Ha ras transformed cells revealed that Raf 1 co immunoprecipitated with endogenous c N ras but not ( G12V ) Ha ras . 0.96639262^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Ha ras , raf 1 and 5 abl ) are thought to code for proteins that participate in growth factor induced signal transductions . . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Identification of discrete segments of human Raf 1 kinase critical for high affinity binding to Ha Ras . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Isolation of a new protein factor required for activation of Raf 1 by Ha Ras : partial purification from rat brain cytosols . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| We observe that activation of ERK 1 via Raf 1 and p21ras dependent signals can result in the hyperphosphorylation of c jun . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Quantitative analysis of the complex between p21ras and the Ras binding domain of the human Raf 1 protein kinase . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| The protein Raf 1 , a key mediator of mitogenesis and differentiation , associates with p21ras ( refs 1 3 ) . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Normal and oncogenic p21ras proteins bind to the amino terminal regulatory domain of c Raf 1 . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Interferon gamma activation of Raf 1 is Jak 1 dependent and p21ras independent . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Peroxynitrite activates ERK via Raf 1 and MEK , independently from EGF receptor and p21Ras in H9C2 cardiomyocytes . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Three different membrane targeting motifs from K Ras , Fyn , and Src proteins were fused to the N or C terminus of Raf 1 . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Other high level amplified oncogenes included NRAS , RAF 1 , MYB , EGFR , FGF 4 , EMS 1 , and D17S167 . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Treatment of human Raf 1 purified from insect Sf 9 cells co expressing c H Ras and Src ( Y527F ) ( in which phenylalanine replaces tyrosine at residue 527 ) with either serine threonine or tyrosine phosphatases resulted in enzymatic inactivation of Raf 1 . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Northern blot analyses using 11 different oncogene probes revealed that 5 oncogenes , i . e . c myc , c H ras , c sis , c raf , and c fos , were expressed . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Signal transducers included phospholipase C gamma ( PLC gamma ) , protein kinase C gamma ( PKC gamma ) , and two proto oncogenes , c H ras and c raf 1 . ^^^ Three of these proteins , PLC gamma , PKC gamma and Raf 1 , did not transform NIH3T3 cells alone or in combination . c H ras , which under its own promoter control has low transforming activity , also did not cooperate with PLC gamma or PKC gamma . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| After four or eight weeks , the lung tissue of the mice treated with NNK displayed a significantly high level of expression in c myc , c raf , and c H ras oncogenes , and they were all inhibited by tea drinking with inhibitory rates of 50 % , 20 % , and 50 % , respectively . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Apoptosis and cell proliferation were determined by immunocytological techniques and the markers p 53 , p21WAF / Cip , Bax , Bak , Bcl 2 , cyclin D 1 and PCNA , and also screened for signal transduction indicators such as c H ras , c fos and raf 1 . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| The earliest detectable step was activation of Src tyrosine kinases , followed by activation of Ha Ras and Raf 1 . ^^^ The response to UV was blocked by tyrosine kinase inhibitors and dominant negative mutants of 5 src , Ha ras , and raf 1 . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| These oncoproteins , 5 Sis , 5 Src , Ha Ras , and Raf 1 , participate in a signal transduction pathway that leads to increased phosphorylation of serines 63 and 73 on c Jun . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| The expression of mRNAs for c myc , Ha ras , c raf , and the protein phosphatase 2A alpha gene ( PP 2A alpha ) was also examined in the four HCCs by northern blot analysis . ^^^ In contrast , the expression levels of the Ha ras , c raf , and PP 2A alpha genes were the same as those in the livers of control rats . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| A poly ( ADP ribose ) polymerase inhibitor , benzamide ( BA ) , was found to induce flat revertants of NIH 3T3 cells that had been transformed by human Ha ras , rat Ki ras , rat c raf , and human ret 2 . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Elevated expression of protooncogenes c myc , c fos , Ha ras 1 , Ki ras 2 , N myc and Raf 1 was observed in carcinomas of human stomach . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| The transcriptional levels of the type 4 collagenase and the type 4 procollagen gene in the oncogene ( Ha ras , ki ras , raf and myc ) transformed human bronchial epithelial cell lines were compared with the properties of invasiveness in vitro , of tumorigenicity and of metastatic ability in athymic nude mice . 5 Ha ras gene enhanced invasion and metastasis , whereas 5 ki ras , c raf and c myc had lesser effects on these malignant phenotypes . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Moreover , by use of Ha ras and Raf 1 dominant negative mutants , we show that both Ha ras and Raf 1 are required for pX induced activation of c jun transcriptional activity . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| The transformation defect of Ha ras ( G12V , E37G ) is rescued by a mutant , raf 1 , that restores interaction . ^^^ These results indicate that multiple cellular components , including Raf 1 , are activated by Ha Ras and contribute to Ha Ras induced mammalian cell transformation . . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| With the use of dominant negative mutants of Ha Ras and Raf 1 , we investigated some of the early signaling events leading to the activation of NF kappa B by hypoxia . ^^^ Both dominant negative alleles of Ha Ras and Raf 1 inhibited NF kappa B induction by hypoxia , suggesting that the hypoxia induced pathway of NF kappa B induction is dependent on Ras and Raf 1 kinase activity . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Coexpression of dominant inhibitory mutants of Ha ras and raf 1 with MT inhibited MT mediated transactivation and focus formation . ^^^ The dominant inhibitory mutants of Ha ras and raf 1 interfered with the ability of MT to activate c Jun . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Mutations that abolish the ability of Ha Ras to associate with Raf 1 . ^^^ In the present study , we tested 34 mutants of Ha Ras carrying substitution ( s ) in the region of residues 23 71 for their ability to associate with Raf 1 . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| We analysed the mechanisms of c Jun transcription factor activation by pX and in particular the role of cellular proteins involved in the transduction of mitogenic signals ( namely Ha Ras and Raf 1 ) . ^^^ We show by use of Ha Ras and Raf 1 dominant negative mutants that both Ha Ras and Raf 1 are required for pX induced activation of c Jun transcriptional activity . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Despite the similarity in the structure between the JRE IL 6 element and the polyomavirus enhancer PyPEA 3 , composed of an EBS and an AP 1 binding site and known to be activated by a variety of oncogene signals , JRE IL 6 could not be activated by activated Ha Ras , Raf 1 , or 12 O tetradecanoylphorbol 13 acetate . ^^^ The combination of JEBS and the CRE like site appears to form the basis for the selective and efficient response of JRE IL 6 to IL 6 signals , but not to signals generated by activated Ha Ras , Raf 1 , or protein kinase C . . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Finally , since Ha Ras T35A and E37G mutations prevent Ras interaction with full length Raf 1 , we suggest that Raf Cys is a cryptic binding site that is unmasked upon Ras interaction with Raf 1 ( 55 131 ) . . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Similar inhibitory effects were seen with injection of mRNAs encoding dominant negative mutants of c Ha Ras , c Raf , or c Jun . ^^^ DN BR induced dorsalization of the embryo , revealed by the formation of a secondary body axis or dorsalization of the ventral mesoderm explant analyzed by histological and molecular criteria , was significantly reversed by coinjection of [ Val 12 ] Ha Ras , c Raf , or c Jun mRNA . ^^^ Furthermore , the BMP 4 stimulated erythroid differentiation in the ventral mesoderm was substantially inhibited by coinjection with the dominant negative c Ha Ras , c Raf , or c Jun mutant . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Utilizing ASO directed against the raf 1 gene , a central component of this proposed pathway , we were able to reverse the RR phenotype of human tumor cell lines having elevated HER 2 expression or a mutant form of Ha ras , two genes upstream of raf 1 in signal transduction . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| We now report that thyroid stimulating hormone represses mitogen activated protein kinase activity and that microinjection of an effector domain mutant Ha Ras protein , Ras ( 12V , 37G ) , defective in Raf 1 binding and mitogen activated protein kinase activation , stimulates DNA synthesis in quiescent and thyroid stimulating hormone treated thyrocytes . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Coassociation of Rap1A and Ha Ras with Raf 1 N terminal region interferes with ras dependent activation of Raf 1 . ^^^ Ha Ras proteins carrying mutations N26G and V45E , which failed to bind to CRR , also failed to activate Raf 1 . ^^^ Both Rap1A and Ha Ras ( E31K ) mutant failed to activate Raf 1 and interfered with Ha Ras dependent activation of Raf 1 in Sf 9 cells . ^^^ Enhanced binding of Rap1A to CRR led to co association of Rap1A and Ha Ras with Raf 1 N terminal region through binding to CRR and Ras binding domain , respectively . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Here , we examined the activities of various mutants of human Ha Ras to induce membrane translocation of Raf 1 and to activate Raf 1 in vivo . ^^^ Overexpression of an activator region mutant Ha Ras ( V45E ) in COS 7 cells induced membrane translocation of Raf 1 as effectively as wild type Ha Ras . ^^^ However , the activity of this mutant to activate Raf 1 and extracellular signal regulated kinase 2 ( ERK 2 ) was attenuated by approximately 70 % compared to that of wild type Ha Ras . ^^^ The decrease in the specific activity was further demonstrated by measuring the activity of the Ha Ras ( V45E ) associated Raf 1 purified from the membrane fraction . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| The peptide with the D38N mutation inhibited association of Ha Ras with Byr 2 but not with the others , whereas that with the P34G mutation inhibited association of Ha Ras with Raf 1 and Byr 2 but not with adenylyl cyclase . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Ha ras overexpression triggered apoptosis was also prevented in the 7 4 derivatives that express either dominant negative rasAsn 17 or dominant negative raf 1C4B to suppress Ha ras signal transduction at different stages , indicating that overexpression of activated Ha ras can induce cell apoptosis and that raf 1 pathway activity is required for this process . . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| We show here , however , that in vivo there are marked quantitative differences in the ability of Ki and Ha Ras to activate Raf 1 and phosphoinositide 3 kinase . ^^^ Thus , Ki Ras both recruits Raf 1 to the plasma membrane more efficiently than Ha Ras and is a more potent activator of membrane recruited Raf 1 than Ha Ras . ^^^ Interestingly , the ability of Ha Ras to recruit Raf 1 to the plasma membrane is significantly increased when the Ha Ras hypervariable region is shortened so that the spacing of the Ha Ras GTPase domains from the inner surface of the plasma membrane mimicks that of Ki Ras . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| A role for c Raf kinase and Ha Ras in cytokine mediated induction of cell adhesion molecules . ^^^ Using highly specific antisense oligonucleotides that target the mRNA encoding c Raf kinase and Ha Ras , we show here that inhibition of c raf and Ha ras expression blocks the up regulation of E selectin and vascular adhesion molecule 1 induced by TNFalpha in endothelial cells . ^^^ These results indicate that TNFalpha induction of E selectin and vascular adhesion molecule 1 in endothelial cells occurs through signaling pathways that are , at least in part , dependent on c Raf kinase , Ha Ras , and JNK2 . . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Raf 1 is involved in the regulation of the interaction between guanine nucleotide exchange factor and Ha ras . ^^^ This work studies the influence of the Ras effector c Raf 1 on the action of guanine nucleotide exchange factors ( GEFs ) on Ha Ras in vitro . ^^^ Our data show that Raf 1 and phosphatidylinositol 3 kinase can influence the upstream activation of Ha Ras . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Ce AF 6 associated with human Ha Ras in a GTP dependent manner , with an efficiency comparable to that of human Raf 1 Ras binding domain . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Overexpression of dominant negative Raf 1 , Rac 1 , or RhoA in the Ha ras transformants clarified that among these factors , only dominant negative Raf 1 could reverse the cell sensitivity to TNF alpha , indicating that Raf 1 , as a proapoptotic factor , indeed participates in TNF alpha cytotoxicity . ^^^ Taken together , we suggest that the possible action of Ha ras overexpression to sensitize TNF alpha treated fibroblasts is predominantly through the Ras / Raf 1 / MAPK pathway to increase the responses of AP 1 , c fos , and c myc , which are possibly involved in the aberration of cell cycle machinery , and subsequently to turn on the death program . . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Further , the expression of the catalytically inactive mutants of Ha Ras , RhoA , c Raf , and Erk 2 or addition of the Mek 1 specific inhibitor PD 098059 abrogated the stimulation of the urokinase promoter by HGF / SF . ^^^ These data suggest that the induction of urokinase by HGF / SF is regulated by a Grb2 / Sos1 / Ha Ras / c Raf / RhoA / Mek1 / Erk2 / c ++ +Jun dependent mitogen activated protein kinase pathway . . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| The hierarchies were as follows : for Raf 1 activation , Ki Ras 4B > Ki Ras 4A > > > N Ras > Ha Ras ; for focus formation , Ha Ras > / = Ki Ras 4A > > > N Ras = Ki Ras 4B ; for anchorage independent growth , Ki Ras 4A > / = N Ras > > > Ki Ras 4B = Ha Ras = no growth ; and for cell migration , Ki Ras 4B > > > Ha Ras > N Ras = Ki Ras 4A = no migration . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| On the other hand , transient transfection of the dominant negative construct of raf 1 rescued serum deprived Ha ras transformed brown adipocytes from apoptosis , decreasing the percentage of hypodiploid cells , the external display of phosphatidylserine , and the DNA laddering . ^^^ However , inhibition of mitogen activated protein kinase with PD 098059 did not preclude apoptosis and in fact increased the rate of apoptosis observed in serum deprived Ha ras transformed cells , indicating that the Ras / Raf 1 pathway induced apoptosis throughout a mitogen activated protein kinase kinase 1 ( MEK 1 ) independent pathway . ^^^ Thus , we propose a mechanism of apoptosis in Ha ras transformed adipocytes under serum deprivation involving Raf 1 association with phosphorylated Bcl 2 , down regulation of Bcl 2 expression , and up regulation of Bcl xS expression . . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| We demonstrate that transient transfection of oncogenic Ha Ras leads to a preferential activation of endogenous c Raf 1 in HEK 293 cells as opposed to A Raf . ^^^ Mutational analysis reveals that this residue represents a point of isozyme discrimination : c Raf R59K binds Ha Ras weaker than the wildtype , likewise A Raf K22R increases its affinity to Ha Ras in vivo and in vitro . ^^^ Immunecomplex kinase assays reveal that Ha Ras mediated Raf activation is decreased for c Raf R59K and increased for A Raf K22R when compared to the respective wildtype forms . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| We show that a cationic polymer , polyethylenimine ( PEI ) , improves the uptake and antisense activity of 3 ' capped 20 mer and 12 mer antisense phosphodiester oligonucleotides ( PO ODN ) targeted to different regions of Ha ras mRNA and to the 3 ' untranslated region ( 3 ' UTR ) of C raf kinase . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Treatment of COS 7 cells expressing Ha Ras ( Val 12 ) and Raf 1 with TPA enhances the Ha Ras ( Val 12 ) dependent Raf 1 kinase activity . ^^^ In contrast , the Ha Ras ( Val 12 ) dependent activities of the Raf 1 ( S259D ) and Raf 1 ( S259A ) mutants are comparable to that of wild type Raf 1 stimulated by both Ha Ras ( Val 12 ) and TPA and can not be further stimulated by TPA treatment . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Moreover , expression of antisense Ha Ras or dominant negative Raf 1 abrogated the mitogenic effect of TGF beta 1 in TSU Pr 1 , and the TGF beta 1 inhibition of DU 145 was switched to stimulation by V12Ha Ras transfection . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| The linker domain of the Ha Ras hypervariable region regulates interactions with exchange factors , Raf 1 and phosphoinositide 3 kinase . ^^^ N Myristoylation also prevents the release of activated Ha Ras from lipid rafts and inhibits Raf 1 activation . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| One antisense compound has been approved for local treatment of cytomegalovirus induced retinitis , and several others are in clinical trials , including those targeting the mRNA of Bcl 2 , protein kinase C alpha ( PKC alpha ) , c raf or Ha ras . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| These results indicate that Ras , Bcl 2 , as well as Raf 1 and PI3K pathways play pivotal roles in 5 FU induced apoptosis under Ha ras overexpressed condition . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Both p21ras and pp60v src are required , but neither alone is sufficient , to activate the Raf 1 kinase . ^^^ Further , Raf 1 has been shown to act either downstream or independently of the p21ras protein , as indicated by experiments involving microinjection of anti Ras antibodies . ^^^ To investigate the potential role of p21ras in the activation of Raf 1 by tyrosine kinases , we have used the baculovirus / Sf9 cell system to overproduce various wild type and mutant forms of pp60src , p21ras , and Raf 1 proteins . ^^^ When Raf 1 is analyzed by immunoblotting , as previously reported for mammalian cell experiments , a marked increase in the apparent molecular weight of Raf 1 is seen only when it is coexpressed with both pp60v src and p21ras . . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| New perspectives on Raf 1 : the involvement of p21ras in the activation of Raf 1 and a potential role for Raf 1 in events occurring later in the cell cycle . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Here we show that ligation of CD 28 by a monoclonal antibody ( mAb ) also stimulates p21ras and induces Ras dependent events such as stimulation of the microtubule associated protein ( MAP ) kinase ERK 2 and hyperphosphorylation of Raf 1 . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Interleukin 1 activates p 54 mitogen activated protein ( MAP ) kinase / stress activated protein kinase by a pathway that is independent of p21ras , Raf 1 , and MAP kinase kinase . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| The expression of dominant negative Syp blocked the activation of MEK and raf 1 kinase in response to insulin and had no detectable effect on insulin induced activation of p21ras . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Recent studies of growth factor signal transduction pathways demonstrate that Raf 1 functions downstream of activated tyrosine kinases and p21ras and upstream of mitogen activated protein kinase . ^^^ However , coexpression of both activated tyrosine kinases and p21ras is required for maximal activation of Raf 1 in the baculovirus Sf 9 expression system . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Inasmuch as hSOS is involved in p21ras activation , we also demonstrated that mIgM ligation activated a Ras dependent kinase cascade in which sequential activation of Raf 1 and MEK 1 culminates in the activation of p 42 mitogen activated protein ( MAP ) kinase ( ERK 2 ) . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| The TCR regulates the activity of p21ras , and a range of experiments have shown that p21ras couples the TCR to an intracellular kinase cascade involving the serine / threonine kinase Raf 1 and the MAP kinase ERK 2 . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Previous analyses have demonstrated that Ras 1 ( p21ras ) operates upstream of the D Raf ( Raf 1 ) serine / threonine kinase in this signaling pathway . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Using dominant negative mutants of p21ras and raf 1 , we demonstrate that induction of egr 1 after antigen receptor cross linking is mediated by activation of the p21ras / mitogen activated protein kinase signaling pathway . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| G protein motif of CD 3 gamma : relevance for signal transduction Association of lipid kinase with the T cell antigen receptor Intracellular signaling by phospholipid metabolites and calcium : activation of protein kinase C Protein kinase C isoenzymes Heterogenity of protein kinase C and mode of activation Phospholipid derived mediators in activation of protein kinase C in T cells Role of phospholipase D metabolites in activation of protein kinase C Polyunsaturated fatty acids and lysophosphatidylcholine as activators of protein kinase C Potein kinase C and p21ras function in interdependent and distinct signaling pathways during T cell activation Raf 1 kinase : regulator or target of protein kinase C ? ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| In contrast to Raf 1 immunodepleted lysates , preclearance of the cytosolic B Raf significantly reduced , by 96 % , the amount of MEK 1 activity associated with immobilized p21ras . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Recent studies have demonstrated the existence of a physical complex containing p21ras ( RAS ) , p74raf 1 ( RAF 1 ) , and MEK 1 . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| The major signalling pathway employed by these receptors involves the activation of p21ras and raf 1 kinase . ^^^ Analysis of various signalling intermediates shows that cAMP interferes at a site downstream of p21ras , but upstream of raf 1 kinase . ^^^ Also TPA induced , p21ras independent , activation of raf 1 kinase and ERK 2 is inhibited by cAMP . ^^^ We have used the inhibitory effect of cAMP to investigate whether phosphorylation of mSos , a p21ras nucleotide exchange factor , is dependent on the activity of the raf 1 kinase / ERK2 pathway . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| The protein encoded by the c raf 1 proto oncogene is thought to function downstream of p21ras because disruption of Raf blocks signalling by Ras in a number of systems . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Mitogen activated protein ( MAP ) kinases Raf 1 , pp60src , and p21ras all play important roles in the transfer of signals from the cell surface to the nucleus . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Tyrosine kinase oncogenes and receptors and p21ras can activate Raf 1 , and recent studies have suggested that Raf 1 functions upstream of MEK ( MAP / ERK kinase ) , which phosphorylates and activates ERK . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Both p21ras and its downstream Raf 1 kinase participate in the transduction of signals initiated from a variety of cell surface receptors and in the regulation of transcription factors . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Activation of other members of the signal transduction cascade ( Shc , p21ras , Raf 1 , ERK 1 ) revealed similar phosphorylation levels induced by suramin and NGF . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| The cytokine activated tyrosine kinase JAK 2 activates Raf 1 in a p21ras dependent manner . ^^^ In the baculovirus coexpression system , Raf 1 was activated by JAK 2 in the presence of p21ras . ^^^ Under these conditions , a ternary complex of p21ras , JAK 2 , and Raf 1 was observed . ^^^ In contrast , in the absence of p21ras , coexpression of JAK 2 and Raf 1 resulted in an overall decrease in the Raf 1 kinase activity . ^^^ Taken together , these data suggest that JAK 2 and p21ras cooperate to activate Raf 1 . . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Mitogen activated protein ( MAP ) kinases are proline directed kinases which are downstream components of a pathway involving p21ras and the serine / threonine kinase Raf 1 . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| The activation induced phosphorylation of two p21ras effectors , Raf 1 and Erk 2 , was inhibited by the CD 4 ligands , indirectly pointing to inhibition of the p21ras activation pathway . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| AMP suppresses p21ras and Raf 1 responses but not the Erk 1 response to granulocyte colony stimulating factor : possible Raf 1 independent activation of Erk 1 . ^^^ We examined the effect of 8BrcAMP addition on the G CSF stimulated extracellular signal related protein kinase 1 ( Erk 1 ) , p21ras and Raf 1 activation . ^^^ The Erk 1 activity was not down regulated by the increase in intracellular cAMP levels , whereas p21ras and Raf 1 activities were , suggesting that Erk 1 activity might not be dependent on upstream p21ras and / or Raf 1 activity in this system . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Furthermore , HeLa cells lacking Jak 1 displayed no activation of STAT1alpha , STAT 3 , and Raf 1 by IFN beta or OSM and also demonstrated no increase in the relative level of GTP bound p21ras in response to OSM . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| ERK 2 is activated via a kinase cascade initiated by activation of the G protein p21Ras followed by phosphorylation and activation of Raf 1 and mitogen activated protein kinase kinase 1 ( MEK 1 ) . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| This is done by taking the complex between Rap1A ( Rap ) and the p21ras binding domain of c Raf ( Raf RBD ) ( Nassar et al . , Nature 375 : 554 560 , 1995 ) as a model system . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Downstream effector molecules of p21Ras include Raf 1 kinase which mediates external signals via kinase signaling pathways to nuclear transcription factors including c Fos and c Jun . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Pretreatment of cells with 0 . 5 mM sodium arsenite ( but not other activators of stress activated MAP kinase cascades ) prevents the activation of p21Ras and strongly suppresses the activation of c Raf and the MAP kinase cascade by a variety of growth factors . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Injection of antibodies to p21ras itself , or a recombinant Raf 1 protein domain which binds to the effector region of ras in a GTP dependent manner , results in the inhibition of cell cycle progression throughout G 1 phase . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| We have examined whether the p21ras raf 1 mitogen activated protein kinase / extracellular signal regulated protein kinase ( ERK ) kinase ( MEK ) ERK cascade plays a role in regulating the production of LT , because the activity of these signaling molecules is up regulated in activated T lymphocytes . ^^^ Transfection of Jurkat leukemic T cells with a dominant negative mutant of p21ras ( ras17N or ras15A ) , raf 1 ( raf 1 130 ) , or ERK 1 ( Erk 1 K71R ) resulted in the suppression of the mitogen / phorbol ester stimulated production / secretion of LT . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Although p21ras , raf 1 and MEK have been shown to regulate directly the transcriptional activity of NFAT ( nuclear factor of activated T cells ) and / or the interleukin 2 ( IL 2 ) promoter , direct evidence that the extracellular signal regulated protein kinase ( ERK ) is involved in regulating IL 2 production is still lacking . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Despite the fact that RAF 1 lies immediately downstream of p21RAS in the MAP kinase signalling cascade , recent evidence in non haematopoietic environments suggest that RAS and RAF can transduce signals through alternative pathways specific to a particular cell type . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Despite the involvement of p21ras , the protein kinase Raf 1 is not hyperphosphorylated in response to GHRH , contrary to what usually occurs when the Ras Raf MAP kinase pathway is activated . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Considering the lack of coimmunoprecipitation of p21ras with Raf 1 , the reduction of the level of GTP associated p21ras and the decrease of the activation of the extracellular signal regulated protein kinases ( ERK ) , also known as mitogen activated protein ( MAP ) kinase , in ribavirin treated cells , we suggest a possible relationship between the expression of heat shock proteins and the change , in GTP depleted cells , of the regulation of Raf kinase by ras protein . . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Immunocytochemical detection of p21ras , Raf 1 , ERK1 / MAP kinase and PKC isoforms in a 20 methylcholanthrene induced transformed murine embryonal fibroblast cells in culture . ^^^ An immunocytochemical study using antibodies against p21ras , Raf 1 , MAP kinase / ERK1 and PKCalpha , beta , gamma , delta , epsilon , zeta , isoforms were performed on a 20 methylcholanthrene induced transformed murine embryonal fibroblast cells in both in vitro and in vivo growth conditions . ^^^ Altered expression of p21ras , Raf 1 , MAP kinase in this particular cell line strongly supported the previous findings of the activation of one component of signal transduction under the influence of the other in the MAP kinase cascade of signal transduction during neoplastic transformation and which also seemed to be involved in CNCI PM 20 cell line . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| NO and cGMP dependent activation of p21Ras result in binding of the oncoprotein to the Ras binding domain of Raf 1 kinase . ^^^ It is concluded that NO and cGMP stimulate a signaling pathway involving p21Ras Raf 1 kinase MEK ERK1 / 2 . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Whereas CaMK inhibition affected neither integrin stimulated Akt phosphorylation nor p21Ras or Mek 1 activity , it was necessary for Raf 1 activity . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| The time course of p21ras S glutathiolation following peroxynitrite corresponds to the increase in its Raf 1 binding activity and translocation to the membrane . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| The activity of p21Ras was decreased and Raf 1 abundance increased in PCOS , suggesting that altered mitogenic signaling began at this level . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| The expression of nine proto oncogenes ( c myc , N myc , c fos , c jun , p 53 , H ras , N ras , c raf , hst ) and other three genes ( AFP , PCNA , GST P ) were investigated during spontaneous development to hepatocellular carcinomas ( HCCs ) in LEC rats . ^^^ Expressions of c myc , H ras , N ras , c raf , p 53 , and PCNA genes were detected but did not significantly change during the development to HCCs in LEC rats . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| We have studied the expressions of nine proto oncogenes ( c myc , N myc , c fos , C jun , p 53 , H ras , N ras , c raf , hst ) and two other genes ( PCNA , GST P ) during the spontaneous development of hepatocellular carcinomas ( HCCs ) in LEC rats . ^^^ Expression of c myc , H ras , N ras , C raf , p 53 and PCNA genes was detected , but this did not significantly change during the development of HCCs in LEC rats . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Frequently multiple oncogenes were found expressed although expression levels were rather moderate . 25 30 % of the analyzed tumors showed significant expression of either erbB , src , raf 1 , lck or H ras . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| We obtained evidence that NIH3T3 cells transformed by the c raf or H ras oncogene maintained a decreased level of phosphorylation of the 80K protein , with or without phorbol ester ( TPA ) stimulation , at all concentrations of serum tested while normal NIH3T3 cells maintained an elevated level of phosphorylation of the 80K protein . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Lod scores for close linkage of each candidate sequence to breast cancer were 19 . 6 for HRAS , 12 . 3 for KRAS 2 , 1 . 0 for NRAS , 6 . 0 for MYC , 6 . 1 for MYB , 8 . 2 for ERBA 2 , 7 . 9 for INT 2 , and 5 . 1 for RAF 1 . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Analysis of cellular oncogenes showed that myc and fps were amplified approximately tenfold and threefold , respectively , in this cell line , whereas N myc , L myc , N ras , K ras , H ras , abl , erbB 2 , Blym , src , raf 1 , myb , and sis were not changed significantly . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| We found that in vitro activation of adenylyl cyclase by yeast Ras 2 and human H Ras proteins is subject to competitive inhibition by its leucine rich repeats domain and by the N terminal regulatory domain of human Raf 1 protein . ^^^ The Kd of Raf 1 for the modified H Ras , 3 . 5 nM , was significantly lower than that for Ras 2 , 24 nM , whereas adenylyl cyclase bound preferentially to Ras 2 . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| DNA probes for the NRAS , HRAS , KRAS 2 , LCK , RAF 1 , MET , MYCL 1 , MYCN , MYB , ERBB 2 , FOS , CSF1R , and SRC protooncogene loci ; the retinoblastoma gene locus ( RB 1 ) ; the tumor virus integration sites INT 2 , PVT 1 , and MLV 12 ; and the locus of the tumor specific antigen T1A were used to screen mouse genomic DNAs from RF / J , CAST / Ei , MOLF / Ei , Mus musculus musculus , M . m . poschiavinus , and M . spretus . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| IGF 1 or 10 % FCS , however , similarly increased the mRNA late expression of c myc , H ras , c raf , beta actin , and glucose 6 phosphate dehydrogenase ( G6PD ) at 72 h , as compared to quiescent cells . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Employing the transient expression of dominant negative forms of H Ras and Raf 1 , we determined that the GHR / JAK2 mediated activation of ERK2 / MAPK is dependent on both Ras and Raf . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| By using methods based on inhibition of yeast adenylyl cyclase or RasGAP activity and by in vitro binding assays , we have shown that the segment of B Raf corresponding to amino acid 1 326 binds directly to H Ras with a dissociation constant ( Kd ) comparable to that of Raf 1 and that the binding is not significantly affected by the post translational modifications . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| We examined the effects of the Gly 60 to Ala mutation on the interaction of H Ras with Ras GTPase activating protein ( GAP ) , neurofibromin 1 ( NF 1 ) , Raf 1 , and ral guanine nucleotide dissociation stimulator ( ralGDS ) , factors that interact with GTP bound form of H Ras . ^^^ The G60A mutation moderately reduces the binding of H Ras to Raf 1 Ras binding domain ; however , the binding of H Ras to ralGDS Ras binding domain was more significantly affected by the same mutation . ^^^ These results indicate that although GAP , NF 1 , Raf 1 , and ralGDS all interact with H Ras in a GTP dependent manner and they are able to compete against each other for binding to H Ras , these factors share overlapping but not identical binding domains on H Ras . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| The interactions of H Ras , R Ras , and Rap1A with the Ras binding domains ( RBD ) of the c Raf kinase and of the Ral guanine nucleotide exchange factor ( RGF ) was studied biochemically in solution . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Nine of these genes ( H ras , c myc , c fos , c jun , junB , N myc , c abl , c yes , and p 53 ) were expressed in epithelial cells , whereas two ( RB 1 and N ras ) were not . expression of four other genes ( c src , K ras , c raf , and c myb ) was observed , but the intensity of these bands was too low for densitometric analysis . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| However , whereas constitutively activated mutants of Ras [ H Ras ( 61L ) and K Ras4B ( 12V ) ] and Raf 1 ( DeltaRaf 22W and Raf CAAX ) caused indistinguishable morphologic and growth ( in soft agar and nude mice ) transformation of NIH 3T3 fibroblasts , only mutant Ras caused morphologic transformation of RIE 1 rat intestinal cells . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Here we demonstrate that Rheb interacts with and appears to regulate Raf 1 kinase , an essential component of the H Ras signaling pathway . ^^^ In direct contrast to H Ras , however , the interaction of Rheb with Raf 1 is potentiated by growth factors in combination with agents that increase cyclic AMP ( cAMP ) levels . ^^^ Protein kinase A dependent phosphorylation of serine 43 within the regulatory domain of Raf 1 reciprocally potentiates its interaction with Rheb and decreases its interaction with H Ras . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Using a screen for suppressors of integrin activation , we identified the small GTP binding protein , H Ras , and its effector kinase , Raf 1 , as negative regulators of integrin activation . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| H Ras 12V , 35S retains the ability to activate the Raf / MEK / mitogen activated protein ( MAP ) kinase cascade , whereas H Ras 12V , 40C is unable to interact directly with Raf 1 yet still influences other signaling intermediates , including Rac and Rho . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Assaying ERK 5 activity in immune complexes with one of these substrates , c Myc , we determined that the ERK 5 catalytic domain is activated by V 12 H Ras and to a lesser extent by phorbol ester but not by constitutively active mutants of Raf 1 . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| The sequences of these peptides were derived from the H Ras effector region ( amino acids 25 to 51 ) and the Ras binding domain of Raf 1 ( amino acids 64 to 105 ) . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Like PMA , the H ras oncogene , another activator of the Raf 1 / MEK / MAPK pathway , transactivated p 21 ( WAF1 / CIP1 ) in SKBr 3 cells . 1 further investigated PMA induced growth arrest following infection of SKBr 3 cells with 12S E1A expressing adenovirus . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Anti beta 1 integrin antibody ( clone ; 9EG7 ) , which is known to change integrin conformation and activate integrins , induced the adhesion of dominant negative H Ras transfected Baf 3 cells as much as the other types of H Ras transfected Baf 3 cells . 8 Br cAMP , Dibutyryl cAMP , Ras Raf 1 pathway inhibitors , and PD 98059 , a MAPK kinase inhibitor , suppressed proliferation and phosphorylation of MAPK detected by Western blotting with anti phospho MAPK antibody , but not adhesion of any type of H Ras transfected Baf 3 cells , whereas U 73122 , a phospholipase C ( PLC ) inhibitor , suppressed adhesion of these cells completely . ^^^ These data indicate that H Ras and PLC , but not Raf 1 , MAPK kinase , or the MAPK pathway , are involved in the inside out signaling pathway of IL 3 induced VLA 4 and VLA 5 activation in Baf 3 cells . . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| While activation of the serine / threonine kinases Raf 1 and B Raf has been implicated in signalling pathways leading to transformation by H Ras , it has been argued that TC 21 does not activate Raf 1 or B Raf . ^^^ |
|
| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| On the other hand , a dominant negative mutant of H Ras or that of Raf 1 enhanced the basal and CrkL induced cell adhesion and that of R Ras modestly decreased the adhesion . ^^^ |
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| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Recent clinical studies have tested antisense compounds directed against seven cancer related genes including p 53 , bcl 2 , c raf , H ras , protein kinase C alpha , and protein kinase A . ^^^ |
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| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Moreover , ET 18 OCH3 , an inhibitor of Raf translocation and PI PLC enzyme , which produces the activator of PKC , as well as the antibodies against Raf 1 or MEK also inhibited GnT 5 activity in the parental and H ras transfected cells . ^^^ These results reveal that in parental and H ras transfected 7721 cells , the basal activity of GnT 5 is also regulated by the Ras / Raf 1 / MEK / MAPK cascade in addition to PI 3 K / PKB signaling pathway . ^^^ |
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| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| General serine threonine phosphatase inhibitors such sodium fluoride , or ss glycerophosphate and sodium pyrophosphate , or specific PP 1 and PP2A inhibitors including microcystin LR , protein phosphatase 2A inhibitor 1 ( 1 ) or protein phosphatase inhibitor 2 all abrogate H Ras and K Ras dependent Raf 1 activation in vitro . ^^^ |
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| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| TPA induced phosphorylation of MEK1 / 2 was also raised by OHT treatment , without any change in their protein level or Raf 1 and H Ras levels . ^^^ |
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| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Thus , we propose that H Ras signals followed by Raf 1 / MAPK pathway but not PI3K not only reduces beta ( 1 ) mediated adhesion of osteoblasts to matrix proteins but induces apoptosis presumably via the Fas up regulation and Bcl 2 down regulation . . ^^^ |
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| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Inhibition of endocytosis by dominant interfering dynamin K44A blocked H Ras but not K Ras mediated PC 12 cell differentiation and selectively inhibited H Ras but not K Ras mediated Raf 1 activation in BHK cells . ^^^ H Ras but not K Ras mediated Raf 1 activation was also selectively dependent on phosphoinositide 3 kinase activity . ^^^ Stimulation of endocytosis and endocytic recycling by wild type Rab 5 potentiated H Ras mediated Raf 1 activation . ^^^ In contrast , Rab 5 Q79L , which stimulates endocytosis but not endocytic recycling , redistributed activated H Ras from the plasma membrane into enlarged endosomes and inhibited H Ras mediated Raf 1 activation . ^^^ |
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| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| H Ras can suppress integrin activation in fibroblasts via its downstream effector kinase , Raf 1 . ^^^ Additional data demonstrating that the ability of H Ras or Raf 1 to suppress integrin activation was unaffected by inhibition of bulk ERK1 / 2 MAP kinase activation supported this hypothesis . ^^^ |
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| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Further characterization showed that MCP compounds restore actin stress fibers and cause flat reversion in NIH 3T3 cells transformed with H Ras ( V 12 ) but not in NIH 3T3 cells transformed with constitutively active Raf 1 ( RafDeltaN ) . ^^^ |
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| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Using genomic microarray , we detected oncogene copy number gains of RAF 1 ( 3p25 ) , CTSB ( 8p22 ) , PAK 1 ( 11q13 ) , and JUNB ( 19p13 ) in 5 of 7 cases of mycosis fungoides ( MF ) / Sezary syndrome ( SS ) ( 71 % ) , gains of FGFR 1 ( 8p11 ) , PTPN ( 20q13 ) , and BCR ( 22q11 ) in 4 cases ( 57 % ) , and gains of MYCL 1 ( 1p34 ) , PIK3CA ( 3q26 ) , HRAS ( 11p15 ) , MYBL 2 ( 20q13 ) , and ZNF 217 ( 20q13 ) in 3 cases ( 43 % ) . ^^^ |
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| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Also , we report immunologic localization of H Ras regulatory proteins including its nucleotide exchange factor ( GRF 1 ) and its effector protein ( eg . , Raf 1 ) in isolated beta cells . ^^^ |
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| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Here we report that in fibroblasts , activated H Ras and Raf 1 oncogenes decrease biosynthesis , association with beta 1 integrins , and surface expression of tTG because of down regulation of tTG mRNA . ^^^ In turn , the reduction of surface tTG inhibits adhesion of H Ras and Raf 1 transformed cells on fibronectin and , in particular , on its tTG binding fragment 1 ( 6 ) 2 ( 1 , 2 ) 1 ( 7 9 ) , which does not interact directly with integrins . ^^^ |
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| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Amplification frequencies of 15 genes ( TERC , MYBL 3 , HRAS , PI3KCA , JUNB , LAMC 2 , RAF 1 , MYC , GARP , SAS , FGFR 1 , PGY 1 , MYCL 1 , MYB , FGR ) increased by > 10 % during hormone escape . ^^^ However , downstream receptor tyrosine kinase signaling pathways showed increased amplification rates in resistant tumors for the mitogen activated protein kinase ( FGR / Src 2 , HRAS , and RAF 1 ; P = 0 . 005 ) and phosphatidylinositol 3 ' kinase pathways ( FGR / Src 2 , PI3K , and Akt ; P = 0 . 046 ) . ^^^ |
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| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Rational drug design has resulted in agents directed against a number of important cellular targets , including the mRNA of bcl 2 , protein kinase ( PK ) C alpha , PKA 1 , H ras , c raf , R 1 and R 2 subunit of ribonucleotide reductase , and transforming growth factor beta 2 . ^^^ |
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| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Co transfectants of K Ras / galectin 3 , but not of H Ras / galectin 3 , exhibited enhanced and prolonged epidermal growth factor stimulated increases in Ras GTP , Raf 1 activity , and PI 3 K activity . ^^^ |
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| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Proteins studied included cell surface receptors ( Ephrins and Eph receptors , CD 44 ) , kinases ( EGFR cytoplasmic domain , CDK 2 and 4 ) , proteases ( MMP 1 , CASP 2 ) , signal transduction proteins ( GRB 2 , RAF 1 , HRAS ) and transcription factors ( GATA 2 , Fli 1 , Trp 53 , Mdm 2 , JUN , FOS , MAD , MAX ) . ^^^ |
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| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Moreover , constitutively active H Ras and Raf 1 activating H Ras mutant enhance uPA expression , whereas dominant negative H Ras and Raf 1 block LPA induced uPA up regulation , suggesting that the Ras Raf pathway works downstream of G ( 1 ) to mediate this LPA induced process . ^^^ |
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| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| However , unlike Raf 1 , the phosphorylation of B Raf at Ser ( 445 ) was constitutive and was only moderately increased by expression of constitutively active H Ras or constitutively active PAK 1 . ^^^ |
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| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Since the Ras effector Raf 1 was localized in non GEM / raft , the growth suppression might be caused , at least partly , by the movement of activated H ras to GEM / raft fraction . . ^^^ |
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| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Similarly , mitotic inhibition of ERK activity in cells expressing active mutants of H Ras and Raf 1 kinase could also be reversed following Cdc 2 inhibition . ^^^ |
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| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| In contrast , exogenous expression of constitutively active mutants of Raf 1 and H Ras only partially restored ERK1 / 2 activity , by 50 % and 10 % respectively . ^^^ Assays for GTP loading and H Ras interactions with the Ras binding domain on Raf 1 demonstrated a decrease in H Ras activity in the presence of NO * . ^^^ These findings indicate that intracellular generation of NO * by nNOS leads to S nitrosylation of H Ras , which interferes with Raf 1 activation and propagation of signalling through ERK1 / 2 . . ^^^ |
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| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Since 75 to 90 % of all human pancreatic ductal adenocarcinomas harbor activating mutations at codon 12 of the K ras oncogene it was our belief that Raf 1 MEK MAPK will be activated in the majority of human pancreatic cancers . ^^^ The aim of this study was to confirm activation of Raf 1 in K ras mutant human pancreatic cancer . ^^^ Additionally , we sought to determine if Raf 1 activation differed in K ras mutant and nonmutant pancreatic cancer . ^^^ There was no difference in expression of the 74 kDa activated form of Raf 1 in K ras mutant vs K ras nonmutant cell lines . ^^^ We conclude that Raf 1 is expressed in its active form in human pancreatic cancer regardless of K ras status . ^^^ |
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| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| We have developed a yeast based assay in which the Saccharomyces cerevisiae mating pheromone induced MAPK pathway relied on co expression of K Ras and Raf 1 . ^^^ |
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| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Stimulation of cells with epidermal growth factor ( EGF ) or fetal calf serum ( FCS ) resulted in activation of N Ras , but not K Ras , as well as activation of c Raf , MEK 1 , and p 42 MAPK . ^^^ |
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| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Special features of tumors with mutant K ras included small size of carcinomas compared with carcinomas lacking this mutation , and correlation of proliferating cell nuclear antigen with raf 1 . ^^^ |
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| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Finally , pull down assays using the Ras binding domain of Raf 1 demonstrated that OSM directly activates K Ras in fetal hepatocytes . ^^^ |
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| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Decrease in K ras p 21 and increase in Raf 1 and activated Erk 1 and 2 in murine lung tumors initiated by N nitrosodimethylamine and promoted by 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin . ^^^ Levels of K ras p 21 , raf 1 , mitogen activated protein kinases Erk 1 and 2 , the phosphorylated activated forms of Erk 1 and 2 ( Erk 1P and 2P ) , and proliferating cell nuclear antigen ( PCNA ) were measured by immunoblotting in mouse lung tumors ( 5 to 9 mm in size ) caused by N nitrosodimethylamine ( NDMA ) and in control lungs . ^^^ A significantly greater decrease in membrane associated K ras p 21 and increase in total and membrane associated raf 1 occurred in the NDMA / TCDD tumors compared with the NDMA only tumors . ^^^ These results suggest a possible tumor suppressive function for K ras p 21 in lung and a positive role for raf 1 and Erk 1 / 2 in lung tumorigenesis . ^^^ TCDD may promote tumors by contributing to downregulation of K ras and stimulation of raf 1 . . ^^^ |
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| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| In addition , NAB 2 cells have two other consistent translocations : a 1 ; 5 ( p 22 ; q 23 ) and a 3 ; 7 ( p 25 ; q 22 ) , with breakpoints close to the location of N ras , fms , and raf 1 protooncogenes , respectively . ^^^ In situ hybridization with myc , N ras , and raf 1 radiolabeled DNA probes to NAB 2 chromosomes showed that none of these genes was relocated as a result of translocation . ^^^ |
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| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Expression of the N ras oncogene in embryo fibroblast NIH / 3T3 cells has been demonstrated to increase radioresistance in vitro , and these results have been confirmed and extended to human cell lines expressing the c raf oncogene . ^^^ |
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| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Expression of the proto oncogenes c myc , p 53 , c jun , pim 1 , lck , c syn , c raf and N ras were detected in Hodgkin ' s disease derived cell lines and in normal hematopoietic cells . ^^^ |
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| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Permanent conversion of NIH3T3 cells transformed by activated c Ha ras , c Ki ras , N ras , or c raf , and of human pancreatic adenocarcinoma containing activated c Ki ras to apparently normal cells by treatment with the antibiotic azatyrosine . ^^^ |
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| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| We have analyzed the expression of 12 protooncogenes , c Ha ras , c Ki ras , N ras , c myc , N myc , c fos , c abl , c fes , c fms , c raf , c erbB 1 , and c erbB 2 , in tissues of human renal cell carcinomas and in adjacent normal kidneys . ^^^ Although N ras , c fos , and c raf gene transcripts were detected in both malignant and normal tissues , differences in these protooncogene expressions were not found between the carcinomas and normal kidneys . ^^^ |
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| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Treatment with azatyrosine caused similar conversion of NIH 3T3 cells transformed by activated c Ki ras , N ras , or c raf to apparently normal cells , but NIH 3T3 cells transformed by hst or ret were not exclusively converted by azatyrosine . ^^^ |
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| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| This location demonstrates that Dsi 1 is different from 16 of the known proto oncogenes ( c abl , c erbA c erbB , c ets 1 , c ets 2 , c fes , c fos , c myb , c myc , c raf , A raf , c Ha ras , c Ki ras , N ras , c sis , and c src ) and 12 cellular regions of tumor associated integrations in retrovirus induced tumors ( c erbB , Fis 1 , int 1 , int 2 , Mis 1 / pvt 1 , Mlvi 1 , Mlvi 2 , c mos , c myb , c myc , Pim 1 , and c Ha ras ) . ^^^ |
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| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Four protooncogenes ( c fos , c abl , N ras , and c raf ) were expressed at low levels in both steps of erythroleukemia . c fos and c abl RNAs were barely detectable in normal erythroid cells . ^^^ |
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| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| We have analysed the c myc , c jun , c raf and N ras and p 53 expression in total RNA preparation from tumor biopsies obtained before treatment . ^^^ No significant differences in c raf and N ras expression were found in responding or resistant patients . ^^^ |
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| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| Expression of oncogenes C myc , C raf and N ras in advanced cancers of the upper respiratory and digestive tracts . ^^^ A correlation between oncogenes expression ( N ras , C myc , C raf ) and response to neoadjuvant chemotherapy has not been established in carcinology of the head and neck yet . ^^^ |
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| Interacting proteins: P01112 and P04049 |
Pubmed |
SVM Score :0.0 |
| This included oncogene copy number gains of FGFR 1 ( 8p11 ) in three cases , and NRAS ( 1p13 . 2 ) , MYCN ( 2p24 . 1 ) , RAF 1 ( 3p25 ) , CTSB ( 8p22 ) , FES ( 15q26 . 1 ) , and CBFA 2 ( 21q22 . 3 ) in two cases . ^^^ |
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