Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.56871349
Using a solid phase binding assay , we have demonstrated that TGF beta 1 binds to fibronectin immobilized on Immunlon ITM microtiter plates . 0.56871349^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.53268397
In parallel , TGF beta 1 was expressed by nuclear and occasional anular cells spatially associated with the fibronectin synthesizing cells . 0.53268397^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
The proteins encoded by these clones could be grouped into four distinct classes : extracellular matrix proteins ( fibronectin , beta ig h 3 , collagen 6 ) , enzymes ( collagenase , urokinase ) , cytoskeletal proteins ( vinculin , SM 22 ) and regulatory proteins ( beta glycan , integrin associated protein , myosin kinase , IGFBP 5 ) . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
The downregulated expression of ADAM 9 may serve as a marker for anterior polar cataracts in addition to previously known proteins , fibronectin , alpha SMA , and beta ig h 3 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Synthetic peptides of ERGDEL and GRGDSP derived from beta ig h 3 and fibronectin , respectively , also induced apoptosis , unlike ERGEEL and GRGESP . ^^^ A fusion protein composed of the N terminal 100 amino acids of fibronectin and the RGD containing C terminal part of beta ig h 3 was also subjected to C terminal cleavage and overexpression resulted in apoptosis . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Putative cis regulatory elements were identified by comparing promoter regions from three genes that are highly upregulated in chondroinduced hDFs ( BIGH 3 , COL1A2 , and FN 1 ) [ Zhou , S . , Glowacki , J . , Yates , K . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
We investigated in anesthetized rats whether FN stimulation modifies PIDs expression and , if so , the threshold for evoking cortical spreading depression ( CSD ) , a process sharing characteristics with PIDs and an index of cortical excitability . ^^^ In normal rats , stimulation of FN increased the threshold current for eliciting CSD by 175 % and slowed its propagation velocity by 35 % ( p < 0 . 01 for each ) immediately , but not 72 h , after FN stimulation . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Human Caco 2 enterocytes were cultured on matrix proteins ( collagen 1 , laminin , fibronectin ) with growth factors ( epidermal growth factor [ EGF ] and transforming growth factor beta 1 [ TGF beta 1 ] ) and the tyrosine kinase and prostaglandin inhibitors genistein and indomethacin . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Both the parental and tumorigenic hybrid cell lines were capable of responding to TGF beta as evidenced by increased levels of mRNA for fibronectin , type 4 collagenase , and plasminogen activator inhibitor after treatment with TGF beta 1 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
During this pre confluent stage , TGF beta 1 also increased fibronectin secretion into the culture medium . ^^^ Under these conditions , TGF beta 1 and bFGF had little effect on general protein synthesis , but TGF beta 1 alone caused large , dosage dependent increases in synthesis of fibronectin , and to some extent type 2 and 10 collagens . ^^^ Given together with bFGF , TGF beta 1 synergistically increased secretion of fibronectin . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
While pulse chase studies demonstrated that total ( medium plus cell associated ) collagen levels were stable throughout the 48 h period , TGF beta 1 increased the fraction of cell associated collagen between 24 48 h , and this was partially blocked by alpha amanitin , but not by antibody to fibronectin or beta 1 integrin subunit . ^^^ TGF beta 1 , therefore , has multiple effects on type 1 collagen in fetal bone derived cell cultures , including small increases in mRNA , large increases in polypeptide synthesis , and enhanced association of secreted collagen to the cell layer , which may require synthesis of extracellular components unrelated to fibronectin or the beta 1 integrin subunit . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
To examine this hypothesis , we studied the effect of TGF beta 1 on Fn production by bovine bronchial epithelial cells in culture . ^^^ Fn , released into the media of cultures exposed to TGF beta 1 , increased in a dose and time responsive fashion . ^^^ Fn in the cell layer also increased in response to TGF beta 1 . ^^^ TGF beta 1 also induced an increase in expression of Fn mRNA from cultured bronchial epithelial cells , suggesting that TGF beta modulates Fn production of these cells , at least in part , through modulation of Fn gene expression . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Antagonistic effects of TGF beta 1 and MSF on fibroblast migration and hyaluronic acid synthesis . ^^^ Data presented in this communication indicate that ( a ) MSF specifically stimulates the synthesis of high molecular weight species of HA , ( b ) TGF beta 1 inhibits the elevated migration of adult fibroblasts plated at subconfluent cell density , ( c ) under these conditions , TGF beta 1 induces a parallel decrease in the synthesis of high molecular weight HA and increase in the synthesis of low molecular weight HA , ( d ) TGF beta 1 is a potent antagonist of MSF , effectively blocking its stimulation of cell migration and synthesis of high molecular weight HA , and ( e ) the inhibition of fibroblast migration by TGF beta 1 does not appear to be a chemotactic response dependent upon the existence of a concentration gradient of the cytokine . ^^^ Our results are discussed in terms of the possible combined contribution of MSF and TGF beta 1 to wound healing . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
However , both keloid and normal fibroblasts treated with TGF beta 1 exhibit accelerated fibronectin biosynthesis , indicating that keloid cells can respond to TGF beta 1 . ^^^ In the absence of serum , the TGF beta 1 induced increase in fibronectin biosynthesis occurs more rapidly in keloid fibroblasts , also suggesting modification of this regulatory pathway . ^^^ The TGF beta 1 mediated increase in keloid fibronectin production is independent of the steroid regulatory pathway for fibronectin , which accelerates synthesis by means of a post transcriptional mechanism . ^^^ Thus , TGF beta 1 stimulation of fibronectin production in keloid cells is likely to involve a transcriptional mechanism and keloid overproduction of extracellular matrix components may be due to an inherent modification of the TGF beta regulatory program . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
TGF beta 1 , in the presence of 2 . 5 % donor calf serum stimulated the production of fibronectin and the major collagen types 1 , 3 and 5 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
In TGF beta 1 supplemented cultures ( with , but especially without , serum supplementation ) the epithelia were thin , medial edge epithelial degeneration was marked , and the fibronectin content of the mesenchyme was increased . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Exposure of glomerular epithelial cells to TGF beta 1 increased the production of fibronectin and type 4 collagen , in addition to biglycan . ^^^ TGF beta 1 released in the glomerulus secondary to injury could thus affect both cell types and lead to increased intraglomerular production of proteoglycans , whereas the increased fibronectin , type 4 collagen , and laminin may primarily originate from the epithelial cells . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
In this study , we have investigated the in vivo relationship of the localization of TGF beta 1 to various ECM proteins , including glycosaminoglycans ( GAGs ) , fibronectin , collagens 1 and 3 and to the enzyme alkaline phosphatase , at the early and late bell stages of rat molar tooth development . ^^^ We have used polyclonal antibodies specific to peptides present in the precursor and processed forms of TGF beta 1 , fibronectin and Collagens 1 and 3 and immunohistochemical methods of detection . ^^^ Our results indicate that both the intracellular and extracellular forms of TGF beta 1 are expressed in a site specific manner , and that the growth factor colocalizes with alkaline phosphatase , fibronectin , collagens 1 and 3 and GAGs during the bell stages of tooth development . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
In contrast , TGF beta 1 mimicked most of the effects of PMA by enhancing the attachment of maturating U 937 cells on FN through alpha 5 receptors and by promoting adherence to LM . ^^^ TGF beta 1 stimulation also promoted U 937 cell spreading on both FN and LM coated surfaces . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Cardiac hypertrophy induced changes in mRNA levels for TGF beta 1 , fibronectin , and collagen . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Fibronectin , known to be induced by TGF beta 1 was used as a positive control and tubulin as a negative control . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
When cells were treated with TGF beta 1 for 16 hr prior to exposure to ECM in a 4 hr adhesion assay , a significant increase in adhesion to fibronectin and collagen was observed for the Moser cells . ^^^ The up modulation of adhesion to fibronectin , laminin and collagen by TGF beta 1 was mediated through RGD related integrin receptors . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Immunocytochemistry and ELISA demonstrate 1 to 5 fold higher amounts of desmoplakin and fibronectin after DMF or TGF beta 1 exposure . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
In contrast , the sixfold stimulation of fibronectin production by exposure of cells to 30 pM TGF beta 1 for 48 h was reduced by 50 % in the presence of 3 mM 3 AB , whereas 3 mM 3 ABA had no effect . ^^^ Northern blot hybridization analyses demonstrated that 3 AB , but not 3 ABA , attenuated the induction of fibronectin mRNA by TGF beta 1 by up to 50 % . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
On the other hand , fibronectin expression was increased by TGF beta 1 by about twofold at both early and late times in culture . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Gene transcription , mRNA levels , and message stability of the genes for the extracellular matrix proteins type 1 collagen and fibronectin were also increased by TGF beta 1 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
In 2 D cultures TGF beta 1 is known to increase cellular fibronectin accumulation ; however , in 3 D cultures no difference is seen between control and TGF beta 1 treated cells as established by ELISA testing for type 4 collagen , fibronectin , and laminin . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Transforming growth factor beta ( TGF beta 1 ) suppresses cortisol production when added to cultured bovine adrenocortical ( BAC ) cells while concomitantly increasing fibronectin synthesis and assembly into extracellular fibrils . ^^^ Sodium dodecyl sulfate polyacrylamide gel electrophoresis of gelatin Sepharose treated media from BAC cells demonstrated a 2 fold stimulation of fibronectin production by TGF beta 1 in both the presence and absence of serum . ^^^ Indirect immunofluorescence studies revealed that TGF beta 1 caused a striking increase in the fibronectin content of BAC extracellular matrix . ^^^ TGF beta 1 caused a 4 fold increase in deoxycholate insoluble fibronectin after 12 h and a 7 fold increase after 24 h over that of control BAC cultures . ^^^ Northern hybridization analyses indicated that TGF beta 1 stimulated levels of fibronectin poly ( A ) + RNA 2 . 3 fold . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Full thickness skin biopsies obtained from four patients with rapidly progressive diffuse fasciitis associated with the Eosinophilia Myalgia syndrome ( EMS ) were examined for the expression of transforming growth factor beta 1 ( TGF beta 1 ) , type 6 collagen , and fibronectin genes employing immunohistochemistry and in situ hybridizations . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Neointima formation and TGF beta 1 immunoreactivity were correlated with increases in fibronectin , collagen alpha 2 ( 1 ) , and collagen alpha 1 ( 3 ) gene expression . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
When added exogenously to cultures of mesoderm cells growing with epithelial characteristics on fibronectin , TGF beta 1 effects an epithelial mesenchymal transformation within 24 h . ^^^ TGF beta 1 is also able to down regulate the deposition of fibronectin by mesoderm cells grown on fibronectin and of epiblast cells grown on laminin , but up regulate fibronectin deposition by mesoderm on laminin . ^^^ Expression of the fibronectin receptor is also down regulated by TGF beta 1 in mesoderm cells grown on fibronectin , and this may explain the decreased deposition of fibronectin associated with these cells under these conditions . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Moreover , these hybrids regain full responsiveness to TGF beta 1 , as measured by inhibition of DNA synthesis as well as stimulation of fibronectin and plasminogen activator inhibitor 1 production . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Further studies showed that c myc is not a target for TGF beta 1 in liver epithelial cells and that TGF beta 1 no longer induces fibronectin mRNA in transformed cells . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
TGF beta 1 addition to exponentially growing Mv1Lu cells increased the levels of junB mRNA and of three extracellular matrix proteins : plasminogen activator inhibitor 1 , fibronectin , and thrombospondin . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
The effect of TGF beta 1 on the expression of the basement membrane genes , fibronectin , laminin B 1 , and collagen alpha 1 ( 4 ) , was examined . ^^^ Incremental doses of TGF beta 1 also increased cellular levels of fibronectin protein in undifferentiated keratinocytes and resulted in increased secretion of fibronectin . ^^^ Treatment of these differentiated cells with TGF beta 1 induced the expression of fibronectin mRNA to levels seen in TGF beta treated , undifferentiated keratinocytes but only marginally increased the expression of collagen alpha 1 ( 4 ) and laminin B 1 mRNA . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Treatment of cells with TGF beta 1 resulted in a time and dose dependent enhancement of type 4 collagen and fibronectin gene expression , as detected both at the mRNA level by Northern hybridizations and at the protein level by semi quantitative indirect immunofluorescence analyses . ^^^ Cycloheximide experiments provided evidence that the TGF beta 1 elicited upregulation of the expression of the fibronectin gene is , but that of type 4 collagen is not , entirely dependent on protein synthesis , suggesting two different mechanisms for enhancement of gene expression . ^^^ These studies provide evidence for the role of beta 1 integrins in TGF beta 1 elicited alterations in HT 1080 cell culture morphology , possibly mediated by the expression of ligand proteins for these integrins , such as type 4 collagen and fibronectin . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
In an experimental model of glomerulonephritis in rats we found increased proteoglycan and fibronectin synthesis by cultured nephritic glomeruli , which was greatly reduced by addition of antiserum to TGF beta 1 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
In an experimental model of glomerulonephritis in rats , we found increased proteoglycan and fibronectin synthesis by cultured nephritic glomeruli , which was greatly reduced by the addition of antiserum to TGF beta 1 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
However , nonactivated BON conditioned medium stimulated DNA synthesis , soft agar growth , and an increase in TGF beta 1 and fibronectin mRNA expression in AKR 2B fibroblasts . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
With immunofluorescent staining , the extracellular fibronectin was observed as coarse fibrils only in some cells of the control culture , while cells treated with 20 ng / ml transforming growth factor beta 1 ( TGF beta 1 ) showed intense fluorescences in a radiating pattern around most of the cells , indicating that TGF beta 1 markedly increased fibronectin secretion in keratinocytes . ^^^ Analysis of [ 35S ] methionine labeled proteins also revealed that TGF beta 1 increased fibronectin secretion eight fold in culture medium . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Transforming growth factors beta ( TGF beta 1 , TGF beta 2 ) mediate the inhibition of epithelial cell proliferation and the induction of fibronectin and collagen gene expression . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Since cellular responses to peptide growth factors are mediated , in part , through the regulation of specific gene expression , we have studied the effect of TGF beta 1 on the EGF dependent transcriptional of a diverse panel of genes which included the fibronectin gene , the cytoskeletal beta and gamma actin genes , and the c fos protooncogene . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
PT did not , however , affect TGF beta 1 stimulated fibronectin and collagen mRNA accumulation nor did it have any inhibitory effect on TGF beta 1 induced morphological transformation . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
The more tumorigenic cell lines had very few surface receptors for TGF beta 1 and showed no increase in the secretion of a number of specific proteins , including fibronectin , following TGF beta 1 treatment . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Colocalization of TGF beta 1 and collagen 1 and 3 , fibronectin and glycosaminoglycans during lung branching morphogenesis . ^^^ The possible in vivo role of TGF beta 1 in regulating various proteins of the extracellular matrix , including fibronectin , collagen 1 and 3 , and glycosaminoglycans , was examined by immunohistochemical methods during critical stages of lung morphogenesis in the 11 to 18 day old mouse embryo . ^^^ Sections of Bouin fixed , paraffin embedded whole embryos were exposed to polyclonal antibodies specific to synthetic peptides present in the precursor part of TGF beta 1 ( pro TGF beta 1 ) , in the processed TGF beta 1 ( antibody CC ) , collagen 1 and 3 , fibronectin , followed by the PAP or ABC technique to visualize the location of the antibody . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Stimulation of fibronectin production by TGF beta 1 is independent of effects on cell proliferation : the example of bovine adrenocortical cells . ^^^ In the present study , we addressed the question of whether these cells modify the synthesis of their extracellular matrix ( in particular of fibronectin ) in response to TGF beta 1 , similarly to the changes observed in cell types whose growth is modified by this factor ( e . g . , fibroblasts ) . ^^^ Immunofluorescence studies using anti fibronectin antibodies revealed that TGF beta 1 treatment in serum free medium induced the formation of fibronectin containing fibrils associated with adrenocortical cells . ^^^ Metabolic labeling of adrenocortical cells with [ 35S ] methionine showed that fibronectin synthesis and secretion were highly stimulated by low concentrations of TGF beta 1 . ^^^ Stimulation of adrenocortical fibronectin synthesis by TGF beta 1 appeared to imply a transcriptional event since it was no longer observed in the presence of DRB , a potent inhibitor of RNA polymerases , and because the level of fibronectin mRNA was stimulated under TGF beta 1 treatment . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
We demonstrated that 24 hours of incubation of human dermal fibroblasts with TGF beta 1 ( 1 ng / ml ) under serum free conditions resulted in an elevated expression of TGF beta 1 , collagen alpha 2 ( 1 ) and fibronectin mRNAs that persisted at least 96 hours after removal of TGF beta 1 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Data focus on the stimulatory role of this growth factor in granulation tissue formation , on the enhanced mRNA content of collagen types 1 and 3 , fibronectin , TGF beta 1 itself , and on the reduction in stromelysin mRNA , suggesting that increased matrix formation measured after treatment with TGF beta 1 is due to fibroplasia regulated by the abundance of mRNAs for several different structural , matrix proteins as well as inhibition of proteolytic phenomena elicited by metalloproteinases . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Fibronectin , collagen , and gelatin were also unable to signal changes in receptor expression , even though in other systems such matrix components can regulate the responsiveness of cells to TGF beta 1 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Further analysis indicated that binding to the high Mr proteoglycan is not necessary for TGF beta 1 induced regulation of DNA , collagen and fibronectin synthesis , change in cell morphology , or reorganization of the actin cytoskeleton . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
We confirmed that TGF beta 1 increases the expression of fibronectin , an adhesive glycoprotein expressed during embryogenesis and tissue remodeling . ^^^ Using a monospecific antibody raised to the human placental fibronectin receptor complex , we found that TGF beta 1 stimulated fibronectin receptor synthesis up to 20 40 fold and increases mRNA levels encoding both the alpha and beta subunits up to 3 fold , compared to control IMR 90 in serum free medium . ^^^ The earliest detectable TGF beta 1 mediated increases in fibronectin receptor complex protein synthesis and mRNA levels occur at 8 h , whereas the earliest increases in fibronectin protein synthesis and mRNA levels occur at 12 h . ^^^ These results demonstrate that TGF beta 1 stimulates fibronectin receptor synthesis , extending the diverse stimulatory activities of this polypeptide to matrix receptors . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Human platelet derived transforming growth factor beta ( TGF beta 1 ) increases the accumulation of the extracellular matrix proteins , fibronectin and type 1 collagen , in mesenchymal and epithelial cells . ^^^ To determine the basis for this effect , we have examined the levels of mRNAs corresponding to fibronectin and alpha 2 ( 1 ) procollagen in NRK 49 rat fibroblasts and L6E9 rat myoblasts treated with TGF beta 1 . ^^^ The increase in fibronectin and alpha 2 ( 1 ) procollagen mRNAs was detectable 2 h after addition of TGF beta 1 to the cells and their maximal levels remained constant for several days . ^^^ Actinomycin D , but not cycloheximide , inhibited the increase in fibronectin and alpha 2 ( 1 ) procollagen mRNA levels induced by TGF beta 1 . ^^^ The results indicate that TGF beta 1 controls the composition and abundance of extracellular matrices at least in part by inducing a coordinate increase in the levels of fibronectin and type 1 collagen mRNAs . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Cell shape related induction of TGF beta 1 , collagenase , and TIMP 1 genes appears to be selective , as the levels of mRNA for fibronectin and type 1 procollagen were not significantly altered . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
This increase in transglutaminase activity was paralleled by a TGF beta 1 stimulated increase in fibronectin levels . ^^^ The induction of fibronectin and tissue transglutaminase suggests that the TGF beta 1 pathway of cell death differs from that of normal ectocervical epithelial cell differentiation , which is mediated by epidermal transglutaminase . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Accumulation of fibronectin in articular cartilage explants cultured with TGF beta 1 and fucoidan . ^^^ In these experiments , explants of disease free cartilage cultured in the presence of a combination of TGF beta 1 and the sulfated fucopolysaccharide , fucoidan , accumulated fibronectin at levels comparable to those found in osteoarthritic lesions . ^^^ TGF beta 1 increased fibronectin synthesis , most of which was released to the medium . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
By reverse transcriptase polymerase chain reaction through amplification of the entire fibronectin type 3 ( FN 3 ) splicing domain , two distinct TN isoforms were detected in total RNA isolated from gestational day 21 rat lung explant culture treated with TGF beta 1 and from postnatal day 8 rat lung . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Consequently , we have used this model system to study the effects of repression of autocrine TGF beta 1 activity on the expression of integrin alpha 5 beta 1 and integrin alpha 5 beta 1 mediated cell adhesion to fibronectin . ^^^ The diminished expression of integrin alpha 5 beta 1 on the cell surface led to the reduced adhesion of TGF beta 1 antisense transfected cells to fibronectin . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
The mRNAs for type 1 procollagen and osteonectin were also increased by TGF beta 1 but fibronectin mRNA was decreased . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
An antibody to FGF 2 blocked the mitogenic effect of transforming growth factor beta 1 ( TGF beta 1 ) for MFLCs but not TGF beta 1 induced increase in cellular fibronectin messenger RNA ( mRNA ) . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
TGF beta 1 also induced other extracellular matrix components , fibronectin and laminin . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
The presence of TGF beta 1 and TGF beta 2 in invasive breast carcinomas has been determined and related to pathological features , the presence of fibronectin and tenascin and lymphocyte / macrophage infiltration , using immunohistochemistry . ^^^ Prominent reactivity for TGF beta 1 was associated with lymph node metastasis , ( 0 . 02 > P > 0 . 01 ) , increased detection of cellular fibronectin , fine stromal fibronectin staining , more prominent reactivity for tenascin ( 0 . 02 > P > 0 . 01 ) , the presence of tumour associated macrophage infiltration and altered ratios of CD 4 and CD 8 lymphocyte populations , with CD 8 lymphocytes predominating . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Cells from the injured arteries produced more fibronectin and proteoglycans into the culture medium than the cells from normal arteries and contained more TGF beta 1 mRNA . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
In TGF beta 1 and beta 2 treated cells , a greatly enhanced immunostaining for Tn and three isoforms of Fn was seen both as to the number of positive cells and to the amount of immunoreactive material around them . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
By immunohistochemical and Northern blot analyses , the fibronectin content in carotid intimal and medial layers was greater in the TGF beta 1 group compared with that in the control group . ^^^ TGF beta 1 mainly enhanced the formation of matrix containing fibronectin . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Treatment of trunk neural crest cultures with transforming growth factor beta 1 ( TGF beta 1 ) stimulates them to express both FN and PCol 1 immunoreactivities at levels comparable to those normally seen in cranial cultures and simultaneously decreases their expression of melanin . ^^^ However , trunk neural crest cells are significantly less sensitive to the FN inducing effect of TGF beta 1 than are cranial neural crest cells . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Rather , incubating DA SMC with TGF beta 1 for 22 h decreased the rate of migration of SMC on extracellular matrix substrata composed of fibronectin , vitronectin , laminin , and collagen 1 and 4 . ^^^ TGF beta 1 had little effect on overall DA SMC integrin expression , except for a modest increase in the fibronectin receptor ( alpha 5 beta 1 integrin ) . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Exogenous addition of neutralising antibody to TGF beta 1 plus neutralising antibody to TGF beta 2 reduced the monocyte and macrophage profile , neovascularisation , fibronectin , collagen 3 and collagen 1 deposition in the early stages of wound healing compared to control wounds . ^^^ Exogenous addition of neutralising antibody to TGF beta 1 alone also reduced the monocyte and macrophage profile , fibronectin , collagen 3 and collagen 1 deposition compared to control wounds . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
This enhanced glycanation of CD 44 after the TGF beta 1 , bFGF and combined treatments correlated with a 2 fold increase in the affinity of the proteoglycan for fibronectin but had no influence on the binding to type 1 collagen . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
U 46619 and TGF beta 1 stimulated fibronectin ( Fn ) synthesis in MC , as shown by [ 35S ] methionine incorporation into immunoprecipitable Fn . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Notably , astroglial overexpression of TGF beta 1 consistently induced a strong upmodulation of the extracellular matrix proteins laminin and fibronectin in the CNS , particularly in the vicinity of TGF beta 1 expressing perivascular astrocytes , but was not associated with obvious CNS infiltration by hematogenous cells . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Using in situ reverse transcription , the glomerular cell sites of alpha 1 ( 4 ) collagen , fibronectin , laminin B 1 , angiotensinogen , and TGF beta 1 mRNA synthesis were determined at sequential periods following renal ablation . ^^^ No changes were noted for fibronectin , TGF beta 1 , and angiotensinogen mRNAs . ^^^ At 24 d after ablation , at which time sclerosis is not evident , endothelial cells , particularly in the dilated capillaries at the vascular pole , expressed angiotensinogen and TGF beta 1 mRNAs , as well as fibronectin and laminin B 1 RNA transcripts . ^^^ All receptor blockade inhibited angiotensinogen , TGF beta 1 , fibronectin , and laminin B 1 mRNA expression by the endothelium . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Therefore , the effects of TGF beta 1 on the cellular morphology and the distribution of ECM components such as laminin , tenascin , fibronectin , and types 1 and 4 collagens were investigated in confluent cultures of differentiated PECs during a period of 6 days . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Prop partially inhibited the Iso evoked increases in ANF and FN mRNA , completely prevented the Iso induced changes in TGF beta 1 and SERCA mRNA , but had no effect on the Iso stimulated changes in SK and PNK gene expression . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
The fibronectin mRNA level was increased by exogenous TGF beta 1 treatment in a dose dependent manner in RII positive clones , whereas the control cells remained insensitive . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Furthermore , bFGF and TGF beta 1 have an additive effect on levels of tenascin , but not fibronectin , in the conditioned medium . ^^^ Thus bFGF , alone or in combination with TGF beta 1 , is a potential regulator of tenascin expression in vitro . bFGF may alter not only the relative abundance of tenascin and fibronectin in the extracellular matrix , but also the splice variant of tenascin expressed by a given cell type . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
In both PC 3 and PC 3U cells , TGF beta 1 was found to stimulate the induction of fibronectin and plasminogen activator inhibitor 1 and the expression of junB mRNA , and PMA did not affect these responses . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
In addition , TGF beta 1 up regulates the expression of genes involved in adipogenesis such as fatty acid synthase , glycerol 3 phosphate dehydrogenase , malic enzyme and glucose 6 phosphate dehydrogenase , as well as induces the expression of fibronectin ( specific target gene for TGF beta 1 ) . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
We studied the effect of TGF beta 1 and TFG beta 2 on collagen type 4 and fibronectin ( FN ) synthesis in human glomerular mesangial cells in culture ( GMC ) . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
TGF beta 1 and fibronectin mRNA levels started to increase at 6 hours after injury and remained enhanced until 7 days after injury . ^^^ Treatment with TCV 116 significantly inhibited the induction of mRNAs for c fos , c jun , Egr 1 , ODC , and fibronectin in injured artery , whereas the increase in TGF beta 1 gene expression after injury was not prevented by TCV 116 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Treatment with TGF beta 1 inhibited proliferation and stimulated proteoglycan and fibronectin synthesis in MC3T3 E 1 and MG 63 cells . ^^^ In SaOS 2 cells , however , TGF beta 1 enhanced fibronectin synthesis . ^^^ These observations using clonal osteoblastic cell lines demonstrate that the ability of osteoblastic cells to synthesize bone matrix proteoglycans is associated with the responsiveness of these cells to TGF beta 1 , that the responsiveness of osteoblastic cells to TGF beta 1 in cell proliferation and proteoglycan synthesis correlates with the presence of both types 1 and 2 receptors , and that the effect of TGF beta 1 on fibronectin synthesis can develop with little binding of TGF beta 1 to type 2 receptor if type 1 receptor is present . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
High glucose and TGF beta 1 stimulate fibronectin gene expression through a cAMP response element . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
In SHR , types 1 and 3 collagen mRNAs were increased in the left ventricle , and in contrast , fibronectin , collagen 4 , and transforming growth factor beta 1 ( TGF beta 1 ) mRNAs were increased in aorta , compared with those in Wistar Kyoto rats . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Normal odontoblast and in vitro induced odontoblast like cells expressed transcripts encoding for TGF beta 1 and 3 , BMP 2 and 4 , bone sialoprotein and osteonectin whereas either ubiquitous expression or no expression could be detected for TGF beta 2 , IGF 1 or fibronectin mRNAs . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
At the end point of the treatment , we examined renal function , the gene expressions of TGF beta 1 and extracellular matrix components in the interstitium [ collagen types 1 ( COI ) and 3 ( COIII ) , fibronectin ( FN ) ] and the basement membrane ( COIV and laminin ) , and renal microscopic morphology in rats aged 32 weeks . ^^^ In vehicle treated 32 week old SHRSP with renal dysfunction and nephrosclerosis , renal mRNA levels for TGF beta 1 , COI , COIII , FN , COIV were all several fold higher than in WKY . ^^^ Thus , renal TGF beta 1 gene expression was enhanced in SHRSP , which may contribute to the increased renal expressions of COI , COIII , FN , COIV in SHRSP . ^^^ Treatment with TCV 116 ( 0 . 1 mg / kg / day ) in SHRSP , in spite of no reduction of blood pressure , decreased renal mRNA levels for TGF beta 1 , COI , COIII , FN , COIV , being accompanied by the significant decrease in urinary protein and albumin excretion , blood urea nitrogen and plasma creatinine . ^^^ Treatment with TCV 116 ( 10 mg / kg / day ) in SHRSP decreased mRNAs for TGF beta 1 , COI , COIII , FN and COIV to almost the same levels as WKY , being associated with normalization of urinary protein and albumin excretion and the prevention of nephrosclerosis , as judged by microscopic histological observations . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Additional evidence for abnormal cell ECM interactions playing a role in autoimmune like lesions comes from the recent observations in TGF beta 1 knockout mice , in which mononuclear cell infiltration of the heart and lungs has been corrected by the systemic administration of synthetic FN peptides ( Hines at al . , PNAS 91 : 5187 5191 , 1994 ) . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Both TGF beta 1 ( 71 Trp ) and TGF beta 1 ( delta 69 73 ) increased levels of mRNAs for fibronectin and plasminogen activator inhibitor with Mv1Lu cells , whereas only TGF beta 1 ( 71 Trp ) and not TGF beta 1 ( delta 69 73 ) up regulated the mRNA level of carcinoembryonic antigen in LS 513 cells . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
OBJECTIVE : The aim was to investigate changes in cardiac transforming growth factor beta 1 ( TGF beta 1 ) , fibronectin , and collagen types 1 and 3 mRNA levels in isoprenaline induced cardiac hypertrophy , and the effects of delapril , an angiotensin converting enzyme inhibitor , and TCV 116 , an angiotensin 2 type 1 receptor antagonist , on this hypertrophy . ^^^ With low dose infusion , cardiac TGF beta 1 mRNA was not stimulated throughout the infusion , while fibronectin mRNA and collagen types 1 and 3 mRNAs began to increase at 24 h and 48 h , respectively , after the infusion . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Similar to sarcoid lungs , analysis of lung sections obtained from a murine model of granuloma formation revealed increased expression of fibronectin , collagen , integrin receptors , and TGF beta 1 within granulomas . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Although cytokines or growth factors such as IL 1 beta , TGF beta 1 , TNF alpha or EGF stimulated the secretion of gelatinases A and B from MSF cells , their effects on gelatinase B activity were far less than that of the MCAS 3 conditioned medium . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
In vehicle treated 32 week old SHRSP , urinary albumin excretion per 24 h was about 26 fold greater than that in age matched Wistar Kyoto ( WKY ) rats , and the mRNA levels of renal TGF beta 1 , fibronectin and collagen types 1 and 3 in SHRSP were all several fold higher than those in WKY . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
TGF beta 1 and TGF beta 2 upregulated the FN release in a dose and time dependent manner . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
We determined the effects of TGF beta 1 on type 2 cell expression of fibronectin and surfactant protein C ( SP C ) in vitro . ^^^ TGF beta 1 increased the proportion of type 2 cells with detectable mRNA for fibronectin from 9 to 68 % , as demonstrated by in situ hybridization , and increased the fibronectin mRNA levels 10 fold . ^^^ TGF beta 1 treated cultures had increased immunostaining for fibronectin and increased secretion of metabolically labeled fibronectin . ^^^ These data indicate that TGF beta 1 regulates type 2 cell fibronectin protein an mRNA levels . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
TGF beta 1 increased the mRNA levels for alpha 1 ( 1 ) and alpha 1 ( 4 ) collagens and fibronectin . ^^^ Concomitant treatment with cycloheximide did not prevent the stimulation by TGF beta 1 and resulted in exceptional superinduction for the fibronectin gene . ^^^ These results suggest that TGF beta 1 , which is released in glomerular injury , induces an increase in the transcription of the alpha 1 ( 1 ) and alpha 1 ( 4 ) collagen and fibronectin genes in mesangial cells and may mediate the accumulation of the extracellular matrix in the mesangium . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Transcription rates of cell growth and bone related genes ( including histone H 4 , c fos , c jun , TGF beta 1 , beta 2 macroglobulin , collagen , fibronectin , osteocalcin , osteopontin , and tartrate resistant acid phosphatase ) change as a function of calvarial development from birth to 6 weeks and are selectively modified in osteopetrotic animals . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) and transforming growth factor beta 1 ( TGF beta 1 ) stimulate fibronectin synthesis and the transdifferentiation of fat storing cells in the rat liver into myofibroblasts . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Transforming growth factor beta 1 ( TGF beta 1 ) is a potent cytokine that promotes fibrosis by enhancing the synthesis of extracellular matrix components , including fibronectin and the alpha 5 beta 1 fibronectin receptor . ^^^ The tissue distributions of TGF beta 1 , the TGF beta 1 binding proteoglycan decorin , fibronectin , and the alpha 5 beta 1 fibronectin receptor were assessed immunohistochemically . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Renal mRNA levels for TGF beta 1 ; types 1 , 3 , and 4 collagen ; and fibronectin in DOCA salt hypertensive rats were increased by severalfold ( P < . 01 ) compared with sham operated rats . ^^^ In the aorta of DOCA salt hypertensive rats , TGF beta 1 and fibronectin mRNA levels were increased , but types 1 , 3 , and 4 collagen mRNAs did not increase . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Extracellular matrix genes , fibronectin , osteopontin and alpha 1 ( 1 ) collagen , are altered in their expression pattern which may provide an aberrant matrix environment for mineralization and osteoblast maturation and potentiate the TGF beta 1 response throughout the course of osteoblast differentiation . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
With the C 1 coating 4 . 3 10 10 ( 3 ) cells migrated through the filter without chemotactic stimulation compared to 12 . 8 10 10 ( 3 ) with interleukin 1 beta ( IL 1 beta ) 5 ng / ml , 12 . 2 10 10 ( 3 ) with granulocyte macrophage colony stimulating factor ( GM CSF ) 25 ng / ml , 11 . 7 10 10 ( 3 ) with Fn 20 micrograms / ml , and 9 . 0 10 10 ( 3 ) with transforming growth factor beta 1 ( TGF beta 1 ) 20 ng / ml ( all p < 0 . 01 ) . ^^^ The percentage of cells migrating toward each chemoattractant at its optimal concentration was 64 . 3 % for IL 1 beta , 60 . 8 % for IL 8 , 64 . 7 % for GM CSF , 61 . 0 % for Fn , 58 . 9 % for IL 6 , and 69 . 1 % for TGF beta 1 ( all p < 0 . 01 ) . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
In this study we analyzed the effect of acetaldehyde and transforming growth factor beta 1 ( TGF beta 1 ) on undulin and FN synthesis in cultured fat storing cells ( FSC ) isolated from wedge sections of normal human livers . ^^^ TGF beta 1 induced a dose dependent increase of both undulin and FN synthesis in FSC cultures . ^^^ Furthermore , TGF beta 1 antagonized the inhibitory effect of acetaldehyde on undulin production and potentiated the stimulatory effect of acetaldehyde on FN synthesis . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
METHODS AND MATERIALS : To determine if extracellular matrix mRNA and transforming growth factor beta abundance was affected acutely after irradiation , we measured mRNA levels of collagen 1 ( CI ) , collagen 3 ( CIII ) , collagen 4 ( CIV ) , fibronectin ( FN ) , and transforming growth factor beta ( TGF beta 1 , 2 & 3 ) in mouse lungs on day 1 and day 14 after graded doses of radiation . ^^^ Total lung RNA was prepared and immobilized by Northern and slot blotting and hybridized with radiolabelled cDNA probes for CI , CIII , CIV , FN , TGF beta 1 , 2 & 3 and a control probe encoding for glyceraldehyde 3 phosphate dehydrogenase ( GAPDH ) . ^^^ RESULTS : Changes in the expression of CI , CIII , CIV , FN and TGF beta 1 , 2 & 3 were observed as early as 1 day after exposure . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Antibodies against TGF beta 1 or FN almost completely abolished the chemotactic migration of B 16 BL6 cells to the CM ( B 16 BL6 / fibroblast ) or CM ( TGF beta 1 treated fibroblast ) when these antibodies were co incubated with fibroblasts and either B 16 BL6 or TGF beta 1 . ^^^ Analysis of amounts of TGF beta 1 or FN in various CM using ELISA plates , and using their specific antibodies , revealed that the concentration of TGF beta 1 in the CM ( B 16 BL6 ) was slightly higher than in the CM ( B 16 F1 ) , and the amount of FN in the CM ( B 16 BL6 / fibroblast ) was twice as high as in the CM ( B 16 F1 / fibroblast ) . ^^^ These results suggest that TGF beta 1 released from B 16 BL6 cells can stimulate fibroblasts to produce FN ; consequently , the tumor cells were able to chemotactically migrate toward the released FN , and the differences in invasive and migratory activities towards fibroblasts in B 16 BL6 and B 16 F1 cells may in part be due to the amounts of TGF beta 1 from tumor cells and of FN from TGF beta 1 stimulated fibroblasts . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Immunohistochemical staining showed that two TGF beta 1 induced extracellular matrix components , EDA + fibronectin and versican , were greatly increased in the untreated neointimal lesions , but were almost completely absent from the lesions of the anti TGF beta 1 treated animals . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Neutrophil chemotaxis in response to TGF beta isoforms ( TGF beta 1 , TGF beta 2 , TGF beta 3 ) is mediated by fibronectin . ^^^ A mAb to the cell binding domain ( CBD ) of Fn inhibited the chemotactic response to TGF beta 1 and TGF beta 3 by 63 % and to TGF beta 2 by 70 % , whereas the response to FMLP , a classic chemoattractant , was only inhibited by 18 % . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
We have , therefore , examined the influence of TGF beta 1 on mesangial cell fibronectin synthesis . ^^^ Considering , first , levels of mRNA , TGF beta 1 increased steady state fibronectin RNA in cultured mesangial cells by 1 . 9 times 24 hr after treatment of cycling mesangial cells and by 11 . 8 times in growth arrested cells . ^^^ Fibronectin protein concentrations in cell culture supernatants , determined by immunoprecipitation of supernatants from cells labeled with [ 35S ] methionine and by ELISA , were not increased by treatment with TGF beta 1 . ^^^ Western blots and immunoprecipitation of metabolically labeled cells showed that fibronectin was increased , however , in the deoxycholate insoluble extracellular matrix ( ECM ) of cells stimulated with TGF beta 1 . ^^^ TGF beta 1 altered the physicochemical properties of fibronectin in ECM and supernatant such that the isoelectric point of fibronectin , determined from Western blots of 2D SDS PAGE gels , was reduced so that both became more acidic . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Biol . 151 , 191 203 , 1992 ) that transforming growth factor beta 1 ( TGF beta 1 ) inhibited proliferation , up regulated fibronectin synthesis , and suppressed melanogenesis in a population of quail neural crest cells in vitro . ^^^ The SH EP cell line exhibits primarily nonneuronal traits and responded to TGF beta 1 with increased thymidine uptake after 6 days of culture , increased expression of fibronectin mRNA and protein , and decreased laminin synthesis . ^^^ Neither fibronectin mRNA nor protein was detectable in either TGF beta 1 treated or untreated cultures , although laminin synthesis was upregulated by the growth factor . ^^^ In TGF beta 1 treated cultures of the intermediate SH IN cell line , which has been reported to display both neuronal and nonneuronal characteristics , there was marked flattening of many cells , a steady decrease in thymidine uptake , and increased expression of both fibronectin and laminin . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
In serum deprived confluent cultures of bovine pulmonary artery SMCs , TGF beta 1 treatment increased the steady state levels of the mRNAs of collagen types 5 and 11 , as well as of types 1 and 3 , elastin and fibronectin . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Binding activity in quiescent and activated cells correlated with responsiveness to TGF beta 1 ; TGF beta 1 induced cellular fibronectin mRNA expression only in activated and not quiescent cells . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
TGF beta 1 prevents the down regulation of type 1 procollagen , fibronectin , and TGF beta 1 gene expression associated with 3T3 L 1 pre adipocyte differentiation . ^^^ To begin to elucidate the role of the extracellular matrix in adipogenesis , as well as the mechanism whereby TGF beta 1 inhibits differentiation , we examined the expression of two extracellular matrix genes , type 1 ( alpha 1 ) procollagen and fibronectin , as well as endogenous TGF beta 1 . ^^^ When cells from the control or differentiation groups were treated with TGF beta 1 , there was a 2 5 fold induction of procollagen and fibronectin mRNAs throughout the 6 day time course . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
TGF beta 1 stimulated fibronectin and desmoplakins 1 and 2 only . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
The alteration does not impair TGF beta 1 binding to cell surface receptors or the ability of TGF beta 1 to induce fibronectin or plasminogen activator inhibitor type 1 production . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Under these growth inhibitory conditions , TGF beta 1 is still capable of upregulating the expression of fibronectin mRNA . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
An enzyme linked immunosorbent assay showed that AP 16 cells synthesized fibronectin and laminin , and that the expression of fibronectin was increased by TGF beta 1 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
TGF beta 1 also stimulated the production of laminin and fibronectin and their incorporation into the ECM of primary cultures grown in medium with or without serum . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Since adhesion can be altered through expression of cell surface receptors , binding affinities of adhesion molecules for receptors , or both , we investigated the effect of TGF beta 1 on the binding properties of fibronectin and laminin to their cell surface receptors by saturation binding and Scatchard analyses using radiolabeled fibronectin and laminin . ^^^ TGF beta 1 treatment rapidly up modulated the number of cell surface fibronectin binding sites by 1 . 9 fold . ^^^ Concurrent with receptor modulation , TGF beta 1 induced the secretion of fibronectin and laminin from Moser cells . ^^^ Thus the production of fibronectin and laminin and the expression of their receptors were tightly co regulated by TGF beta 1 . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Extracellular matrix markers of TGF beta 1 activity , a special isoform of fibronectin , tenascin , biglycan and plasminogen activator inhibitor 1 , were significantly elevated in kidneys undergoing fibrosis . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Incubation of TGF beta 1 ( / ) MNLs with selected synthetic peptides corresponding to cell and heparin binding sequences of fibronectin ( FN ) significantly attenuated adhesion of these cells not only to FN but also to endothelial cells in vitro . ^^^ Daily injections of a combination of four synthetic FN peptides that interact with beta 1 integrins and / or cell surface proteoglycans blocked the massive infiltration of MNLs into the heart and lungs of TGF beta 1 ( / ) mice . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
RESULTS : The data indicate that expression in glomeruli of alpha 1 , alpha 5 , and beta 1 subunits paralleled both mesangial content of the ligands for the alpha 1 beta 1 and alpha 5 beta 1 integrins , laminin , collagen and fibronectin , and TGF beta 1 protein ; increasing on day 7 of disease and decreasing toward normal by day 28 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
TGF beta 1 stimulated adhesion of human mononuclear phagocytes to fibronectin and laminin is abolished by IFN gamma : dependence on alpha 5 beta 1 and beta 2 integrins . ^^^ In this study , we analyzed the capacity of TGF beta 1 and IFN gamma to regulate adhesion of human activated monocytes to fibronectin ( FN ) and to laminin ( LM ) , two components of the extracellular matrix . ^^^ Exogenous TGF beta 1 increased the adhesive ability of monocytes to FN and to LM , respectively , linked to the increase of alpha 5 and beta 2 mRNA and protein synthesis levels . ^^^ Furthermore , when stimulated with TGF beta 1 , IFN gamma pretreated monocytes reacquired the ability to bind to FN and LM . ^^^ Conversely , IFN gamma reduced adhesiveness to FN and LM of monocytes initially stimulated with TGF beta 1 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Cholesterol , macrophages , and gene expression of TGF beta 1 and fibronectin during nephrosis . ^^^ We also assessed whether cholesterol feeding , which raises the glomerular M phi number , alters the glomerular mRNA levels for TGF beta 1 and fibronectin . ^^^ Glomerular mRNA levels for TGF beta 1 and fibronectin in nephrotic rats exhibited a biphasic temporal pattern , decreasing significantly below control at 3 and 7 days after PA but increasing significantly at 11 and 42 days after PA . ^^^ The upregulated gene expression for TGF beta 1 and fibronectin at 11 days after PA temporally corresponded to the phase of mesangial M phi infiltration in this model . ^^^ Cholesterol feeding to both normal and nephrotic rats significantly increased glomerular TGF beta 1 and fibronectin mRNA levels at 11 days after PA . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
In this study , the effect of TGF beta 1 on procollagen types 1 , 3 , 4 , laminin ( Lam ) , and fibronectin ( FN ) synthesis in cultured human FSCs was analyzed . ^^^ TGF beta 1 induced a dose dependent increase of procollagen 1 , 3 , and FN accumulation in human FSC cultures , whereas procollagen 4 and Lam production was not affected . ^^^ Furthermore , TGF beta 1 increased the expression of alpha 1 ( 1 ) , alpha 1 ( 3 ) procollagen , FN and TGF beta 1 mRNA in human FSC cultures . ^^^ CONCLUSIONS : These data indicate that TGF beta 1 is able to increase the synthesis of procollagen 1 , 3 , and FN in cultured human FSCs . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Although H ras expression in IEC 18 cells causes resistance to TGF beta mediated growth inhibition , the cells remain responsive to TGF beta 1 stimulation of fibronectin expression . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
This effect could not be reversed by astrocyte migration inducing factors such as transforming growth factor beta 1 ( TGF beta 1 ) or by any of the tested extracellular matrix ( ECM ) components ( laminin and fibronectin ) except for vitronectin ( Vn ) . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Furthermore , the motility of HOON and NALM 6 cells on immobilized fibronectin , which appears to be alpha 4 beta 1 integrin mediated , was stimulated two to threefold by TGF beta 1 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
We report additive / synergistic effects of platelet mitogens ( PDGF and TGFb 1 ) as well as bFGF , with ECM proteins ( laminin , fibronectin , collagen 1 and vitreous deposited ECM ) on RPE proliferation . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
The ability of RA to upregulate fibronectin and thrombospondin expression in NRP 152 cells was also blocked by the monoclonal antibody . 1 , 25 ( OH ) 2D3 , which also induced TGF beta s 2 and 3 but not TGF beta 1 , and their respective mRNAs , also induced fibronectin and thrombospondin through induction of TGF beta . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
In the renal cortex of nephritic rats there was a significant increase in gene expression of TGF beta 1 , fibronectin and collagens , and ACE activity . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
The influence of transforming growth factor beta 1 ( TGF beta 1 ) and of dexamethasone on fibronectin ( FN ) synthesis of human renal tubular epithelial cells in culture ( TEC ) was studied . ^^^ Cocultivation with TGF beta 1 increased the steady state level of FN RNA within 24 to 48 hours . ^^^ In TGF beta 1 treated cells , a small increase of FN in the cell supernatants was seen ( 1 . 7 fold ) , and a more prominent increase in the cell lysates ( 4 . 5 fold ) . ^^^ The FN content of the extracellular matrix was also increased in TGF beta 1 treated cells . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
The mRNA expressions of fibronectin or TGF beta 1 were not affected by a 2 week LPD in the Adriamycin rats at any stage . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Diabetic mice given IgG demonstrated total kidney and glomerular hypertrophy , significantly elevated urinary TGF beta 1 protein , and increased mRNAs encoding TGF beta 1 , type 2 TGF beta receptor , alpha 1 ( 4 ) collagen , and fibronectin . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
When cultured on FN , melanoma cells expressed IL 1beta and IL 6 transcripts in addition to IL 1beta , IL 8 , ECGF , TGF beta 1 , TGF beta 2 and TGF beta 3 , already present in quiescent cells . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
TGF beta 1 increases fibronectin expression and urokinase plasminogen activator ( uPA ) activity and plasminogen activator inhibitor 1 ( PAI 1 ) levels in 3D cultures . ^^^ The inhibitory effect of TGF beta 1 on proliferation is suppressed in transfected 2D cultures expressing a truncated form of the type 2 receptor , while its stimulatory effect on fibronectin production is reduced in both 2D and 3D transfected cultures expressing a truncated form of the type 1 receptor . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Additional responses of U 937 cells to TGF beta 1 , including stimulation of fibronectin synthesis , cellular adhesion , platelet / endothelial cell adhesion molecule 1 ( PECAM 1 ) phosphorylation , and homotypic aggregation were also inhibited in the endoglin transfectants . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Immunostaining for transforming growth factor beta 1 ( TGF beta 1 ) was present in germ cells , Sertoli cells and Leydig cells ; vimentin immunostaining was found mainly in Sertoli cells ; desmin immunostaining was found in the peritubular cells , and immunostaining for type 4 collagen , laminin and fibronectin was found in the extracellular matrix of the lamina propria . ^^^ The volume densities of seminiferous tubules ( including seminiferous epithelium , lamina propria and tubular lumen ) that immunostained for TGF beta 1 , vimentin , laminin , desmin or fibronectin were calculated . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
TGF beta 1 induced a more marked increase in collagen and fibronectin release and greater production of sulphated GAGs as DS and heparan sulphate ( HS ) in the otosclerotic cells . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
To determine whether the structural and functional defects were developmental due to the absence of TGF beta 1 or secondary to the inflammation , TGF beta 1 / mice were treated with synthetic fibronectin peptides , which block leukocyte infiltration . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Moreover , in vitro investigations evidenced a TGF beta 1 dependent regulation of alternative splicing of fibronectin mRNA . ^^^ To investigate a possible implication of these growth factors in the tissue formation process of palmar fibromatosis , TGF beta 1 / 2 and bFGF synthesis , as well as TGF beta 1 / 3 and bFGF tissue distribution , is demonstrated by RNA in situ hybridization and / or immunohistochemistry in relation to myofibroblast phenotype development ( alpha smooth muscle actin , desmin immunohistochemistry ) , expression of different fibronectin isoforms ( ED A+ , ED B+ and oncofetal glycosylated fibronectin immunohistochemistry , fibronectin RNA in situ hybridization ) and cellular activity ( cyclin RNA in situ hybridization , Ki 67 immunolabelling ) . ^^^ The myofibroblast phenotype ( alpha smooth muscle actin , desmin ) , the growth factor synthesis ( TGF beta 1 and 2 , bFGF ) , fibronectin matrix synthesis ( RNA in situ hybridization with cDNA ) and ED A+ , ED B+ and oncofetal glycosylated fibronectin immunostaining are exclusively localized in the active proliferative nodules ( Ki 67 immunolabelling and cyclin mRNA demonstration ) . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
During the bone matrix formation / maturation period ( days 10 16 ) , type 1 collagen expression and biosynthesis , fibronectin , TGF beta 1 and osteonectin expressions were high and maximal around day 16 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
TGF beta 1 reduced anti CD 3 activated alpha 4 beta 1 integrin expression and constitutive adhesion to fibronectin , while initial alpha 5 beta 1 expression was heightened and adhesive function sustained . ^^^ Fibronectin based RGD peptides that bind alpha 5 beta 1 integrins and alpha 5 or beta 1 integrin chain specific Abs blocked TGF beta 1 dependent proliferation , while connecting segment 1 peptide that binds alpha 4 beta 1 integrin and alpha 4 chain specific Abs had no effect . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
TGF beta 1 response as assessed by induction of fibronectin expression was higher ( 3 . 0 fold ) in exponentially growing cells than in quiescent cells . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Protein and mRNA expression of TGF beta isoforms , TGF beta 1 , beta 2 and beta 3 , and deposition of fibronectin containing extra domain A ( fibronectin EDA+ ) and plasminogen activator inhibitor 1 ( PAI 1 ) were studied in human chronic glomerulonephritis and diabetic nephropathy . ^^^ While glomerular and tubulointerstitial deposition of two matrix components induced by TGF beta , fibronectin EDA+ and PAI 1 , was significantly elevated in all diseases with matrix accumulation , correlation analysis revealed a close relationship primarily with TGF beta 1 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
RESULTS : Fascial fibroblasts displayed 1 . 75 to 4 . 6 fold greater collagen biosynthesis , 3 . 4 to 8 . 5 fold elevation of the steady state levels of fibronectin mRNA , up to 3 . 9 fold elevation of the steady state mRNA levels for alpha 1 ( 1 ) , alpha 1 ( 3 ) and alpha 3 ( 6 ) collagens , and a marked increase in TGF beta 1 mRNA levels compared to fibroblasts from the adjacent dermis from the same individuals . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Addition of TGF beta 1 to serum free cultures resulted in a dramatic transformation of corneal keratocytes to spindle shaped , fibroblast like cells that expressed alpha SM actin in 100 % of cells and exhibited a 20 fold increase in fibronectin synthesis and a 13 fold increase in alpha 5 beta 1 integrin synthesis . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Ang 2 alone ( 1 microM ) added to quiescent , murine mesangial cells in serum free , insulin free media slightly but not significantly increased TGF beta 1 , fibronectin , collagen 1 , collagen 4 and laminin message levels . ^^^ In contrast , significant increases in mRNA for collagen 1 ( 6 fold ) , collagen 4 ( 4 fold ) , fibronectin 1 ( 4 fold ) and TGF beta 1 ( 2 fold ) were seen with insulin alone ( 10 ( 6 ) M ) in rat mesangial cells , and a dose response effect could be demonstrated for insulin ( 10 ( 9 ) to 10 ( 6 ) M ) . ^^^ Ang 2 plus insulin further significantly increased collagen 1 ( 9 fold ) , collagen 4 ( 9 fold ) , fibronectin 1 ( 5 fold ) and TGF beta 1 ( 3 fold ) message expression . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Present findings suggest a beneficial role for TGF beta 1 during wound healing in providing the acquisition of a migratory phenotype , with a higher capacity to migrate either on collagen or on different extracellular matrix components including laminin and fibronectin . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Condensations form following activation of at least three pathways : ( 1 ) Initiation of epithelial mesenchymal interactions by tenascin , BMP 2 , TGF beta 1 and Msx 1 and 2 . ( 2 ) Up regulation of N CAM by activin . ( 3 ) Up regulation of fibronectin by TGF beta , further enhancing N CAM accumulation ( Fig . 3 ) . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
The cryptic migration stimulating activity of GBD was not affected by the presence of serum or native fibronectin , but was inhibited by TGF beta 1 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Because alveolar type 2 cells in vitro express FN , we examined the splice variants in two conditions that induce FN [ transforming growth factor beta 1 ( TGF beta 1 ) treatment and time in culture ] . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Using an adhesion assay , the authors then investigated the effects of cRA and transforming growth factor beta 1 ( TGF beta 1 ) on cellular adhesion to purified type 4 collagen , fibronectin , and laminin matrices . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Hepatocytes plated onto other collagen containing substrates ( collagen 4 , Matrigel , or ECL , an entactin collagen 4 laminin matrix ) , but not on fibronectin or laminin alone , also displayed enhanced EGF stimulated motility by TGF beta 1 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
TGF beta 1 stimulates cultured human fibroblasts to proliferate and produce tissue like fibroplasia : a fibronectin matrix dependent event . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
After the treatment , the aorta and superior mesenteric artery were excised , and subjected to the extraction of RNA . mRNA levels for the transforming growth factor beta 1 ( TGF beta 1 ) and extracellular matrix components such as fibronectin ( FN ) , collagen type 1 ( CoI ) , type 3 ( CoIII ) and type 4 ( CoIV ) and laminin , were measured by northern blot analysis , using each specific cDNA probe . 3 . ^^^ In the aorta , only TGF beta 1 and fibronectin mRNAs were increased in SHRSP , but collagen and laminin were not increased . 4 . ^^^ In the mesenteric artery of SHRSP , TGF beta 1 mRNA levels were increased compared with Wistar Kyoto ( WKY ) rats , being accompanied by a significant increase in mRNA levels for FN , CoI , CoIII , CoIV and laminin . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
PURPOSE : To examine the effect of TGF beta 1 and post transurethral resection of bladder tumor ( TURBT ) urine on fibronectin ( FN ) expression by transitional carcinoma ( TCC ) cell lines . ^^^ MATERIALS AND METHODS : Northern analysis and rtPCR for mRNA transcripts , in combination with ELISA and immunohistochemistry for protein , were used to quantify the effect of TGF beta 1 and post TURBT urine on cellular expression of FN in two TCC lines , 253J and 647V . ^^^ TGF beta 1 and post TURBT urine increased FN expression at both the mRNA and protein levels in the 253J line . ^^^ Anti TGF beta 1 antibodies inhibited the effect of post TURBT urine on FN expression in 253J cells . ^^^ High levels of TGF beta 1 decreased FN expression in 647V cells . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
We also report on the effects of TGF beta 1 on fibronectin synthesis of diabetic smooth muscle cells by use of ELISA and immunoprecipitation , in order to clarify the role of TGF beta fibronectin pathway in forming characteristic changes of diabetic smooth muscle cells . ^^^ These changes were accompanied by increased fibronectin synthesis in diabetic smooth muscle cells in response to TGF beta 1 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Oral feeding of LY 333531 prevented the increased mRNA expression of TGF beta 1 and extracellular matrix components such as fibronectin and alpha 1 ( 4 ) collagen in the glomeruli of diabetic rats in parallel with inhibition of glomerular PKC activity . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Treatment of NSCLC cells with TGF beta 1 and PMA resulted in a persistent increase in the expression of fibronectin mRNA and protein . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Addition of cycloheximide , a protein synthesis inhibitor , upregulated PAF induced fibronectin mRNA expression but downregulated PAF induced TGF beta 1 gene expression , suggesting the existence of different regulatory transcriptional factors of the two proteins . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
We have demonstrated that transient overexpression of active , but not latent , TGF beta 1 resulted in prolonged and severe interstitial and pleural fibrosis characterized by extensive deposition of the extracellular matrix ( ECM ) proteins collagen , fibronectin , and elastin , and by emergence of cells with the myofibroblast phenotype . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Glomerular TGF beta 1 , fibronectin and collagen 4 mRNAs in nephrectomized rats were significantly higher than those in sham operated rats . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
To investigate the relationship between plasminogen activator ( PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) and the extracellular matrix in malignant and normal lung epithelial cells and to determine whether malignant lung epithelial cells may be more invasive than normal lung epithelial cells because of differences in expression of these proteins in response to TGF beta , the regulation of PA , PAI 1 , fibronectin , laminin and thrombospondin by TGF beta 1 in human non small cell lung cancer ( NSCLC ) cells was examined and compared with normal human bronchial epithelial ( NHBE ) cells . ^^^ Treatment of NSCLC cells with TGF beta 1 resulted in a persistent increase in expression of the mRNAs for fibronectin , laminin and thrombospondin ; expression of fibronectin protein also increased after treatment with TGF beta 1 in these cells . ^^^ When NHBE cells were similarly cultured in the presence of TGF beta 1 , expression of fibronectin mRNA also increased in a persistent manner ; however , only an early transient increase in the level of the mRNAs for laminin and thrombospondin was detected in these cells . ^^^ These data show that there is differential regulation of the genes for PA and PAI 1 and the extracellular matrix protein fibronectin in response to TGF beta 1 not only when NSCLC and NHBE cells are compared , but also when different NSCLC cells are compared with each other . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
The mRNA and / or protein levels of IGF 1 , IGF 2 , IGF receptors , IGF binding proteins ( IGFBPs ) , TGF beta 1 and the ECM components fibronectin , laminin , and collagen 4 were measured , together with cell proliferation . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Our results suggest that the activities of genes for proteins involved in the structure ( Type 1 collagen and fibronectin ) , turnover ( collagenase and TIMP 1 ) and regulation ( TGF beta 1 ) of extracellular matrix ( ECM ) , are all governed at least in part by the status of the cytoskeleton . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
ME24t6 cells did not respond to exogenous TGF beta 1 as measured by inhibition of proliferation or fibronectin ( FN ) induction , indicating that the truncated T beta RII acted as a dominant negative inhibitor of both the growth inhibitory and extracellular matrix ( ECM ) stimulatory TGF beta effects . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
To test this hypothesis , we measured mRNA encoding TGF beta 1 , the TGF beta Type 2 receptor and fibronectin , a key matrix component of the TGF beta 1 tissue response , after incubation of mesangial cells with glycated albumin . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Stimulation of fibronectin mRNA by TGF beta 1 was also attenuated in cells overexpressing PKI . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Activity of cathepsin B and collagenase in urine and excretion of fibronectin and TGF beta 1 in urine of patients with membranous glomerulonephritis ] . ^^^ We aimed to estimate urine cathepsin B , collagenase activity and urine excretion of TGF beta 1 and fibronectin in MG . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
The differential use of VLA 4 and VLA 5 correlated with an increase in fibronectin surface expression by stromal cells in response to TGF beta 1 . ^^^ These results indicate that regulation of fibronectin expression on BM stromal cells by TGF beta 1 results in a modulation of the pattern of integrins used by the precursor and myeloma cells to adhere to BM stroma , which could have important consequences on the proliferation and differentiation of hematopoietic precursor cells as well as on the localization and growth of myeloma cells . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Urinary excretion of TGF beta 1 , PDGF BB and fibronectin in insulin dependent diabetes mellitus patients . ^^^ We studied the urinary excretion of transforming growth factor beta 1 ( TGF beta 1 ) , platelet derived growth factor BB ( PDGF ) and fibronectin ( FN ) in 104 patients ( 52 normoalbuminuric , 24 microalbuminuric , and 28 with overt diabetic nephropathy ) with insulin dependent diabetes mellitus ( IDDM ) of a long duration and in 30 non diabetic controls . ^^^ IDDM patients had higher urinary excretion of TGF beta 1 , PDGF and FN compared to controls . ^^^ Urinary excretion of TGF beta 1 and PDGF was elevated in all IDDM subgroups , while FN excretion was significantly increased only in patients with macroalbuminuria . ^^^ Urinary excretion of TGF beta 1 and FN did not differ between normoalbuminuric IDDM patients with long duration of diabetes , a group at low risk of ever developing diabetic nephropathy , and IDDM patients with incipient or overt diabetic nephropathy . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Stimulation with Ang 2 increased mRNA expression of TGF beta 1 , fibronectin and type 1 collagen . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
FK 506 treated kidneys had a progressive increase in the expression of TGF beta 1 and matrix proteins ( biglycan , tenascin , fibronectin , and type 1 collagen ) . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Expression of mRNA for fibronectin , TGF beta 1 , types 1 and 3 collagen was studied by Northern blot analysis . ^^^ On the other hand , mRNA expression for fibronectin and TGF beta 1 were much lower in the imidapril group than in the control group . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
In the present study we investigated the ability of pirfenidone to moderate fibronectin synthesis by cultured human retinal pigment epithelial ( RPE ) cells maintained in media containing 1 % foetal bovine serum when stimulated with TGF beta 1 . ^^^ RESULTS : Transforming growth factor beta 1 ( 1 10 ng / mL ) increased the production of soluble fibronectin , while pirfenidone ( 300 micromol / L ) significantly reduced the TGF beta 1 induced synthesis of fibronectin . ^^^ CONCLUSION : We conclude that the anti fibrotic effect of pirfenidone may be partly mediated through inhibition of TGF beta 1 induced fibronectin synthesis . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
In the adjacent noninfarcted myocardium , mRNA levels for alpha skeletal actin , atrial natriuretic polypeptide ( ANP ) , transforming growth factor beta 1 ( TGF beta 1 ) , fibronectin , and collagen types 1 and 3 increased significantly . ^^^ In the remote noninfarcted myocardium , mRNA levels for alpha skeletal actin , ANP , and collagen types 1 and 3 increased , while mRNA levels for beta myosin heavy chain , TGF beta 1 and fibronectin did not change . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Concurrently , the expression of pro alpha 1 ( 1 ) collagen , fibronectin , and plasminogen activator inhibitor 1 ( PAI 1 ) was also quantified by hybridization of total mRNA from TGF beta 1 ( + / + ) and TGF beta 1 ( / ) embryonic fibroblasts . ^^^ Further , TGF beta 1 null fibroblasts accumulated and synthesized lower constitutive levels of pro alpha 1 ( 1 ) collagen , fibronectin , and PAI 1 mRNA . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Lastly , in explants treated with TGF beta 1 an increment in the level of fibronectin was demonstrated by means of Western blotting analysis . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Northern blot analyses probing for fibronectin , transin , TIMP 1 and TGF beta 1 were carried out on RNA extracted from mesangial cells exposed to individual and combinations of cytokines . ^^^ However , when mesangial cells were exposed to TGF beta 1 in combination with either TNF alpha or IL 1 beta then fibronectin levels were synergistically up regulated approximately fivefold over unstimulated levels . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
The HGF strains produced greater amounts of TGF beta 1 and TGF beta 2 ( P < or = 0 . 003 ) as well as FN ( P < or = 0 . 04 ) and type 1 collagen ( P < or = 0 . 03 ) ( measured by specific ELISA ) than the GN strains . ^^^ Treatment of HGF fibroblasts with anti TGF beta 1 , beta 2 , or beta 3 , as well as a combination of all 3 antibodies , decreased their FN production by up to 60 % ( P < or = 0 . 04 ) , and was able to decrease FN production by HGF fibroblasts to the levels of the GN fibroblasts . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Expression of transforming growth factor beta 1 ( TGF beta 1 ) , the only known inducer of CTGF so far , as well as of the CTGF target genes collagen 1 alpha 1 , fibronectin and integrin alpha 5 revealed a strong correlation with the expression of CTGF . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
OPC 15161 suppresses the proliferation of Tenon ' s capsule fibroblasts and the production of type 1 collagen and fibronectin stimulated by TGF beta 1 in vitro . ^^^ TGF beta 1 enhanced the TCF production of procollagen 1 and fibronectin . ^^^ OPC 15161 significantly decreased the procollagen 1 content in both the medium , in the cell lysate of TGF beta 1 stimulated cells , and fibronectin content in the lysate . ^^^ CONCLUSIONS : OPC 15161 inhibited the proliferation of TCFs , and reduced their production of procollagen 1 and fibronectin in the presence of TGF beta 1 without evidence of cytotoxicity . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Thus TNF alpha and TGF beta 1 modulate both ECM synthesis and fibronectin content in coal dust exposed type 2 cell cultures . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
RESULTS : The production of laminin and fibronectin was stimulated by treatment with TGF beta 1 or TGF beta 2 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
RESULTS : Organ culture in TGF beta 1 , beta 2 , or beta 3 induced myofibroblast transformation of corneal endothelial cells , with formation of stress fibers containing alpha SM actin , loss of normal pericellular ZO 1 organization , development of extracellular fibronectin fibrils , and formation of focal contacts as indicated by punctate vinculin staining . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Fibronectin synthesis stimulated with 10 microg ml ( 1 ) of saponin was inhibited with 69 % by 5 microg ml ( 1 ) of an anti TGF beta 1 antibody . mRNA of TGF beta type 1 receptor increased 4 . 8 and 4 . 4 fold at fibroblasts treated with 1 and 10 microg ml ( 1 ) of saponin , respectively , and that of TGF beta type 2 receptor also increased 3 . 4 and 3 . 2 fold at fibroblasts treated with 1 and 10 microg ml ( 1 ) of saponin , respectively . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
The expression of TGF beta 1 , beta 2 and beta 3 , TGF beta type 1 and type 2 receptors , alpha 3 , alpha 5 , beta 4 integrin subunits , laminin and fibronectin was studied immunohistochemically . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
To gain better understanding of the temporal relationships between high glucose ( HG ) and mesangial cell ( MC ) TGF beta 1 synthesis and between TGF beta 1 and extracellular matrix ( ECM ) synthesis , the present study examined early and sequential effects of HG on TGF beta 1 and fibronectin ( FN ) mRNA expression and protein synthesis . ^^^ CONCLUSIONS : The present finding demonstrate that HG stimulates TGF beta 1 very early and prior to FN production and that HG induced FN production is mediated by TGF beta . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Tolazamide significantly stimulated transforming growth factor beta 1 ( TGF beta 1 ) secretion and the total synthesis of collagen and collagen and fibronectin accumulation in the medium of MCs maintained in high or low glucose concentrations . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
At concentrations of 300 and 1000 microg / ml , heparin inhibited fibronectin mRNA levels in TGF beta 1 ( 6 ng / ml ) stimulated cells . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Urine activity of cathepsin B , collagenase and urine excretion of TGF beta 1 and fibronectin in membranous glomerulonephritis . ^^^ The purpose of the study was to estimate urine cathepsin B , collagenase activity and urine excretion of TGF beta 1 and fibronectin in MG . ^^^ Urine excretion of fibronectin ( 45 . 60 ng / mg creatinine vs control 10 . 30 ng / mg creatinine ; P < 0 . 04 ) and TGF beta 1 levels in the urine were higher than in controls ( 283 . 55 + / 248 . 13 pg / ml vs 36 . 11 + / 48 . 01 pg / ml ; P < 0 . 01 ) . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Addition of authentic TGF beta 1 to parental cells greatly stimulated secretion of PAI 1 but not FN , whereas addition of TGF beta antibody or lipofection with specific antisense TGF beta 1 oligonucleotides to EGR 1 regulated cells completely inhibits the secretion of PAI 1 but not FN . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
In addition , TGF beta RII / Fc diminished the TGF beta 1 induced production of EIIIA positive fibronectin in cultured normal rat kidney cells . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Expression of N cadherin , N CAM , fibronectin and tenascin is stimulated by TGF beta 1 , beta 2 , beta 3 and beta 5 during the formation of precartilage condensations . ^^^ However , little is known about the relationship between adhesion molecules ( N cadherin and N CAM ) , extracellular matrix proteins ( fibronectin and tenascin ) and TGF beta 1 , TGF beta 2 and TGF beta 3 during in vitro precartilage condensations in mouse chondrogenesis . ^^^ Results showed that TGF beta 1 , TGF beta 2 , TGF beta 3 , and TGF beta 5 differentially enhanced the expression of N cadherin , N CAM , fibronectin and tenascin in precartilage condensations , suggesting that TGF beta isoforms play an important role in the establishment of cell cell and cell extracellular matrix interactions during precartilage condensations . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Enhanced expression of glomerular TGF beta 1 and fibronectin mRNAs and proteinuria were employed as indices of diabetic nephropathy . ^^^ Four weeks after streptozotocin , diabetic rats ( n = 6 : plasma glucose 516+ / 12 mg / dl ) exhibited 6 . 1 fold increase in urinary protein excretion , 1 . 4 fold increase in glomerular TGF beta 1 mRNA , 1 . 7 fold increase in glomerular fibronectin mRNA , 2 . 2 fold increase in plasma lipid peroxides ( LPO ) , and 44 fold increase in urinary LPO excretion above the values in control rats ( n = 6 : plasma glucose 188+ / 14 mg / dl ) . ^^^ Chronic administration of melatonin ( n = 6 ) and taurine ( n = 6 ) prevented increases in glomerular TGF beta 1 and fibronectin mRNAs and proteinuria without having effect on blood glucose . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
OBJECTIVE AND DESIGN : The abilities of osteogenic protein 1 ( OP 1 ) and TGF beta 1 to affect cartilage damage caused by fibronectin fragments ( Fn fs ) that are known to greatly enhance cartilage proteoglycan ( PG ) degradation were compared . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
After 3 , 6 , 9 , and 12 days cells were counted , and TGF beta 1 and fibronectin was measured in supernatants . ^^^ TGF beta 1 and fibronectin may be involved in this act of `` self activation ' ' . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
TGF beta 1 markedly increased the levels of fibronectin , type 1 collagen , and alpha SMA mRNA in bovine lens epithelial explants and intact rabbit lenses . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
One cell line however , HTh 7 , did not respond to TGF beta 1 with growth inhibition , induction of the extracellular matrix protein fibronectin or immediate early genes junB , Smad 6 and Smad 7 mRNA . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Accumulation of fibronectin , collagen type 1 and type 4 was noted in the interstitium by immunofluorescence microscopy in association with enhanced expression of mRNA for these ECM components and their regulatory molecules such as matrix metalloproteinase ( MMP 2 ) , tissue inhibitor of metalloproteinase ( TIMP ) 1 and TGF beta 1 both in glomeruli and cortex . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Significant increases in mRNA for fibronectin ( 7 . 5 fold , P < 0 . 01 ) , heparan sulfate proteoglycan core protein ( 53 fold , P < 0 . 001 ) , laminin beta 1 ( 12 fold , P < 0 . 001 ) , collagen alpha 1 ( 4 ) ( 17 fold , P < 0 . 001 ) , collagen alpha 1 ( 3 ) ( fourfold , P < 0 . 001 ) , and MMP 9 ( twofold , P < 0 . 05 ) were observed after perfusion with TGF beta 1 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Hepatocyte growth Factor / Scatter factor ( HGF / SF ) is a regulator of fibronectin splicing in MDCK cells : comparison between the effects of HGF / SF and TGF beta 1 on fibronectin splicing at the EDA region . ^^^ HGF / SF treatment increased the ratio of EDA + FN mRNA to mRNA of FN that lacks EDA ( EDA FN ) ( EDA+ / EDA ratio ) more than TGF beta 1 treatment did : at a range from 0 . 02 to 20 ng / ml , HGF / SF increased the ratio in a dose dependent manner by up to 2 . 1 fold compared with nontreated control , while TGF beta 1 stimulated the EDA+ / EDA ratio by 1 . 5 fold at the optimum dose of 10 ng / ml . ^^^ However , TGF beta 1 increased total FN mRNA levels by 3 fold at 10 ng / ml , but HGF / SF did not . ^^^ Furthermore , at low cell density , HGF / SF stimulated EDA inclusion into FN mRNA more effectively than did TGF beta 1 , whereas at high cell density , TGF beta 1 was more potent than HGF / SF . ^^^ Simultaneous treatment of cells with HGF / SF and TGF beta 1 synergistically stimulated EDA inclusion into FN mRNA . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Role of fibroblasts in HGF / SF induced cohort migration of human colorectal carcinoma cells : fibroblasts stimulate migration associated with increased fibronectin production via upregulated TGF beta 1 . ^^^ The synergistic stimulation of L 10 cell cohort migration by HGF / SF and TGF beta 1 was associated with increased production of motility enhancing EDA+FN by L 10 cells , and blocking FN with a specific antibody effectively inhibited the synergistic effect . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Lung fibroblasts upregulate tenascin and fibronectin production when treated with TGF beta 1 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
METHODS : The synthesis of the basement membrane ( BM ) proteins entactin , laminin , and collagen type 4 and of the extracellular matrix ( ECM ) proteins tenascin and fibronectin with or without TGF beta 1 stimulation was analyzed by immunostaining , immunoprecipitation of endogenously labeled proteins and Northern blot analysis of total RNA extracted from freshly isolated or cultured SECs from rat or guinea pig livers . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Direct respiratory tract micro injection of adenovirus over expressing active TGF beta 1 resulted in a dose dependent inhibition of epithelial surfactant protein ( SP ) C and SP B mRNA levels by up to 76 % and 70 % , respectively , while in contrast , fibronectin and matrix Gla protein ( MGP ) mRNA levels remained stable . ^^^ However , lungs that had been submerged in adenovirus expressing active TGF beta 1 demonstrated a concentration dependent induction of both fibronectin and MGP mRNA levels up to 4 . 3 and 4 . 7 fold respectively in the presence of 1 10 10 ( 11 ) pfu / ml active TGF beta 1 virus . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
RESULTS : The mRNAs encoding TGF beta 1 ( in both mouse and human ) , entactin , fibronectin , and the collagen alpha 1 ( 4 ) and alpha 2 ( 4 ) chains were significantly induced in total kidney as a function of Alport renal disease progression . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
RNA expression of the extracellular matrix proteins thrombospondin 1 , fibronectin , collagen type 1 , and biglycan as well as expression of the cytokine TGF beta 1 was determined by Northern blotting . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
We conclude that regulation of tTgase expression is important for controlling matrix storage of latent TGF beta 1 complexes and that fibronectin may be one extracellular component to which LTBP 1 is crosslinked when LTBP 1 and tTgase interact at the cell surface . ( J Histochem Cytochem 47 : 1417 1432 , 1999 ) . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
The aims of our study were to ascertain whether epidermal growth factor ( EGF ) , transforming growth factor beta 1 ( TGF beta 1 ) , interleukin 1alpha ( IL 1alpha ) , or interleukin 1beta ( IL 1beta ) can modify MIA PaCa 2 ( pancreatic cancer cell line ) and CAPAN 1 ( metastatic pancreatic cancer cell line ) adhesion to fibronectin , laminin , or type 1 collagen , and whether these cytokines can shift the membrane expression of the hyaluronic acid receptor ( CD 44 ) . ^^^ TGF beta 1 reduced MIA PaCa 2 adhesion to type 1 collagen , but enhanced CAPAN 1 adhesion to fibronectin and laminin . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
In controls , there were relatively low signals of alpha 1 ( 4 ) collagen , alpha 4 ( 4 ) collagen , S laminin , and TGF beta 1 mRNAs , but there were no fibronectin or TGF beta 2 transcripts in glomerular cells . ^^^ In MN , the number of alpha 4 ( 4 ) collagen , alpha 1 ( 4 ) collagen , S laminin or TGF beta 1 mRNA expressing cells per glomerular cross section was significantly larger than in controls ( p < 0 . 05 ) , and fibronectin mRNA was occasionally expressed in glomerular visceral epithelial cells ( GECs ) . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
A causal relationship between oxidative stress and diabetic nephropathy has been established by observations that ( 1 ) lipid peroxides and 8 hydroxydeoxyguanosine , indices of oxidative tissue injury , were increased in the kidneys of diabetic rats with albuminuria ; ( 2 ) high glucose directly increases oxidative stress in glomerular mesangial cells , a target cell of diabetic nephropathy ; ( 3 ) oxidative stress induces mRNA expression of TGF beta 1 and fibronectin which are the genes implicated in diabetic glomerular injury , and ( 4 ) inhibition of oxidative stress ameliorates all the manifestations associated with diabetic nephropathy . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
To develop a RT PCR assay for evaluating gene expression concerning renin ( REN ) , angiotensinogen ( ATG ) and the following ECM components : transforming growth factor beta 1 ( TGF beta 1 ) , fibronectin ( FN ) , matrix metalloproteinase 2 ( MMP 2 ) and tissue inhibitor of metalloproteinases 2 ( TIMP 2 ) ; 2 . ^^^ To assess the influence of renal mass reduction ( RMR ) caused by subtotal ( 5 / 6 ) or partial ( 2 / 6 ) nephrectomy on gene expression for TGF beta 1 , FN , MMP 2 and TIMP 2 ; 3 . ^^^ Quantitation of renal gene expression for REN , ATG , TGF beta 1 , FN , MMP 2 and TIMP 2 was performed using RT PCR assay and comparing amounts of respective gene mRNA with house keeping gene mRNA encoding L 19 ribosomal protein . ^^^ Long term treatment with enalapril prevents an early increase in renal TGF beta 1 and FN gene expression , retards the progression of chronic renal failure caused by critical renal mass reduction , and prolongs survival . 4 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Under high glucose , TGF beta 1 and fibronectin mRNA and proteins were upregulated . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Type 1 collagen and fibronectin , as well as transforming growth factor beta 1 ( TGF beta 1 ) , were increased at day 5 in rats treated with rSPARC ( N = 4 , P < 0 . 05 vs . delivery buffer ) , but only minimal effects were seen on mesangial cell and endothelial cell proliferation . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
In cerebral microvessels from 3 to 4 month old TGF beta 1 transgenic mice , which display a prominent perivascular astrocytosis , levels of the basement membrane proteins perlecan and fibronectin were severalfold higher than in vessels from nontransgenic mice . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
SMC fibronectin ( Fn ) production was measured ( by means of Western blotting ) in SMCs stimulated for 72 hours with TGF beta 1 or EGF . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
TGF beta 1 stimulation of fibronectin transcription in cultured human lung fibroblasts requires active geranylgeranyl transferase 1 , phosphatidylcholine specific phospholipase C , protein kinase C delta , and p 38 , but not erk1 / erk2 . ^^^ In the present work , we demonstrate that TGF beta 1 produces a fourfold increase in transcription of the fibronectin gene in cultured human fetal lung fibroblasts with only a small increase in mRNA stability resulting in a significant increase in fibronectin mRNA steady state level . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Although fibronectin expression peaked at 12 weeks and correlated with that of TGF beta 1 , its elevated expression tended to be prolonged . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
In in vitro culture system , fibronectin synthesis was stimulated by transforming growth factor beta 1 ( TGF beta 1 ) in SMC from OLETF rats , but not in those from LETO rats , suggesting that SMC from OLETF rats respond to TGF beta 1 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Treatment of diabetic mice with antisense ODN partially but significantly decreased kidney TGF beta 1 protein levels and attenuated the increase in kidney weight and the alpha 1 ( 4 ) collagen and fibronectin mRNAs . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
The cortical mRNA expressions of both TGF beta 1 and fibronectin were significantly elevated in L NAME rats . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Isolated glomeruli were used for mRNA extraction or were placed in culture for determination of production of TGF beta 1 , the matrix protein fibronectin , and the protease inhibitor plasmin activator inhibitor type 1 ( PAI 1 ) by enzyme linked immunosorbent assay . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Effects of TGF beta 1 were examined by DNA synthesis in hepatocytes and by measurement of fibronectin production in HSC after CCl ( 4 ) administration . ^^^ Fibronectin production of both normal and injured HSC was affected by TGF beta 1 treatment . ^^^ In HSC , whenever TGF beta 1 is increased , TGF beta 1 can transduce its signal for fibronectin production via its receptor because signalling receptors are expressed constantly . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
To clarify the role of rhEPO in the development of stenosis of AV fistulae , this study examined expression of the erythropoietin receptor ( EPO R ) , TGF beta 1 , plasminogen activator inhibitor type 1 ( PAI 1 ) , cellular fibronectin containing an extra domain A ( EDA+ ) , and TGF beta 1 mRNA , and assessed in situ rhEPO binding in tissue specimens from seven cutaneous veins and eight patent and seven stenosed portions of AV fistulae of patients undergoing dialysis . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
To determine EGR 1 functions , we re expressed EGR 1 in human glioblastoma U 251 cells and found that the secretion of transforming growth factor beta 1 ( TGF beta 1 ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and fibronectin ( FN ) was greatly enhanced . ^^^ Addition of anti TGF beta antibodies completely inhibited the secretion of PAI 1 , but had little effect on secretion of FN , indicating that PAI 1 is under the control of EGR 1 induced TGF beta 1 . ^^^ These results suggest that EGR 1 may regulate cell interaction with the extracellular matrix by coordinated induction of TGF beta 1 , FN , and PAI 1 in human glioblastoma cells . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
This study was aimed to establish the temporal correlation between TGF beta 1 expression , the expression of the extracellular matrix protein fibronectin , and plaque development during atherogenesis of hypercholesterolemic rabbits . ^^^ These results implicate a role for TGF beta 1 in modulating fatty streak formation and the synthesis of extracellular protein fibronectin during plaque development . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
TGF beta 1 , TGF beta receptor 2 and ED A fibronectin expression in myofibroblast of vitreoretinopathy . ^^^ Electron microscopy , immunohistochemistry , and confocal microscopy with antibodies recognizing beta smooth muscle ( SM ) actin , desmin , TGF beta 1 , TGF beta receptors 1 and 2 , and ED A FN were performed . ^^^ The current results suggest that the presence of alpha SM actin positive myofibroblasts is probably dependent on the concomitant neoexpression of TGF beta 1 , TGF beta RII , and ED A FN . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
RESULTS : HG up regulated the expression of monocyte chemotactic peptide 1 ( MCP 1 ) , transforming growth factor beta 1 ( TGF beta 1 ) , and fibronectin messenger RNAs ( mRNAs ) and proteins . ^^^ HG induced activation of diacylglycerol PKC ( DAG PKC ) plays a major role in up regulation of MCP 1 , TGF beta 1 , and fibronectin synthesis by HPMC cultured under HG . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
In this study , we show that , TGF beta 1 treatment enhanced the amount of alpha 5 beta 1 integrin on cell surface , the mRNA level of alpha 5 subunit , and subsequently stimulated cell adhesion onto a fibronectin ( Fn ) and laminin ( Ln ) matrix in SMMC 7721 cells . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
We also demonstrate that TGF beta 1 enhanced the formation of the structural elements important in myofibroblast contractile force generation and transmission , including stress fibers , vinculin containing fibronexus adhesion complexes , and fibronectin fibrils , and that this enhancement occurred prior to , and independent of , alpha sm actin expression . ^^^ Removal of TGF beta 1 resulted in reduced expression of alpha sm actin as well as a decreased assembly of stress fibers and vinculin containing adhesion complexes ; however , there was no reduction in fibronectin fibrils . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
We studied , in human mesangial cells , the effect of stretch on p 38 MAP kinase activation and the role of p 38 MAP kinase in stretch induced fibronectin and transforming growth factor beta 1 ( TGF beta 1 ) accumulation . p 38 MAP kinase was activated by stretch in a rapid ( 11 fold increase at 30 min , P < 0 . 001 ) and sustained manner ( 3 fold increase at 33 h , P < 0 . 001 ) ; this activation was mediated by protein kinase C ( PKC ) . ^^^ Stretch induced fibronectin and TGF beta 1 protein levels were completely abolished ( 100 % inhibition , P < 0 . 001 ; and 92 % inhibition , P < 0 . 01 , respectively ) by SB 203580 , a specific p 38 MAP kinase inhibitor . ^^^ At 33 h , TGF beta 1 blockade did not affect stretch induced fibronectin production , but partially prevented stretch induced p 38 MAP kinase activation ( 59 % inhibition , P < 0 . 05 ) . ^^^ TGF beta 1 induced fibronectin accumulation after 72 h of exposure via a p 38 MAP kinase dependent mechanism ( 30 % increase over control , P < 0 . 01 ) . ^^^ In human mesangial cells , stretch activates , via a PKC dependent mechanism , p 38 MAP kinase , which independently induces TGF beta 1 and fibronectin . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
In this paper we show that , compared with administration of Blm by itself , when a synthetic peptide of CD 36 between amino acids 93 and 110 is given concomitantly with Blm to rats , alveolar macrophages generate markedly less active TGF beta 1 , the rats gain weight more rapidly , and there is less inflammation , collagen 1 and 3 , and fibronectin synthesis . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Our results showed that genistein and curcumin blocked this response in a dose dependent manner and also inhibited the TGF beta 1 induced synthesis of fibronectin , an early responsive gene to the growth factor . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NO production , physiologic parameters , and histology were studied in addition to the mRNA expression of TGF beta 1 , plasminogen activator inhibitor 1 ( PAI 1 ) and the matrix proteins biglycan and collagens type 1 and 4 by Northern and the protein expression of PAI 1 and fibronectin by enzyme linked immunosorbent assay . ^^^ In addition , the CsA induced up regulated expression of TGF beta 1 , PAI 1 , and the matrix proteins biglycan , fibronectin , and collagen 1 was significantly increased with L NAME and strikingly improved with L Arg . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
METHODS : We examined the sequential changes of urinary albumin excretion ( UAE ) , morphology , and glomerular mRNA expression for TGF beta 1 and fibronectin ( FN ) in relation to glomerular mRNA expression for angiotensinogen ( ATN ) , angiotensin converting enzyme ( ACE ) , angiotensin 2 type 1a ( AT1a ) , and type 1b ( AT1b ) receptors , and intervention with angiotensin 2 type 1 receptor antagonist candesartan and equihypotensive hydralazine . ^^^ Northern blot analysis showed that glomerular levels of TGF beta 1 and FN mRNA were higher in SHRSP than in WKY rats at all time points . ^^^ While candesartan reduced mRNA levels for RAS components , TGF beta 1 , and FN to control levels , hydralazine was not effective in this respect . ^^^ Conclusion Results suggest that increases in glomerular RAS components that occur independently of circulating RAS alter glomerular permselectivity and increase the glomerular expression of TGF beta 1 and FN in young SHRSP . ^^^ Findings in old SHRSP suggest that altered glomerular permselectivity and an increased glomerular expression of TGF beta 1 and FN may be associated with the activation of systemic RAS . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
We have investigated the effect of transforming growth factor beta 1 ( TGF beta 1 ) on the in vitro adhesion activity of the rat osteosarcoma cell lines ( ROS 17 / 2 . 8 ) to extracellular matrix substrata , including fibronectin , type 1 and 4 collagen , as well as laminin . ^^^ The results showed that incubation with various concentration of TGF beta 1 ( 1 15 ng / ml ) significantly increased the adhesion activity ( 1 . 4 to 2 . 5 folds ) of ROS 17 / 2 . 8 to fibronectin and type 1 collagen ( p < 0 . 01 ) , whereas the adhesion activity to laminin and type 4 collagen was slightly elevated ( 1 . 1 to 1 . 5 folds ) . ^^^ Treatment with Arg Gly Asp Ser ( RGDS ) and rhodostomin effectively suppressed the TGF beta 1 enhanced adhesion activity to fibronectin and type 1 collagen . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
CONCLUSIONS : Our results demonstrate that TGF beta 2 and TGF beta 1 modified the alternative splicing pattern of fibronectin pre mRNA and enhanced the synthesis and secretion of this extracellular matrix molecule by trabecular cells in a dose dependent fashion . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
The effects of all three substances on matrix synthesis were evaluated by immunoblot analyses and ELISA for collagen type 1 and fibronectin after stimulation with TGF beta 1 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
The expression of TGF beta 1 protein and the production of extracellular matrix such as FN , Co1A1 were markedly decreased in the antisense TGF beta 1 transfected cultured cells by ELISA , immunohistochemistry and in situ hybridization . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Second , in an approach to quantify and to study possible relations between eosinophilic inflammation and changes in extracellular tissue components we measured interleukin 5 ( IL 5 ) , eotaxin , eosinophil cationic protein ( ECP ) , leukotrienes ( LTC4 / D4 / E4 ) , transforming growth factor beta 1 ( TGF beta 1 ) , fibronectin , hyaluronic acid , and albumin in nasal tissue homogenates of 31 subjects . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Furthermore , addition of TGF beta 1 specifically induced morphological changes compatible with a shift to a mesenchymal phenotype , accompanied by loss of ZO 1 at cell borders and up regulation of the mesenchymal markers alpha smooth muscle actin and fibronectin . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Runx 2 mimicked common effects of TGF beta 1 and BMP 2 by inducing expression of matrix gene products ( for example , collagen and fibronectin ) , suppressing MyoD expression , and inhibiting myotube formation of C2C12 cells . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Cells that were exposed to high glucose exhibited significant increases in ERK activity , TGF beta 1 expression ( total protein , mRNA levels , and promoter activity ) , [ ( 3 ) H ] proline uptake , and alpha 1 ( 1 ) collagen and fibronectin mRNA levels . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
The HG induced generation of ROS was , in turn , related to activation of PKC and transcription factors nuclear factor kappaB ( NF kappaB ) and activator protein 1 ( AP 1 ) as well as to the up regulation of transforming growth factor beta 1 ( TGF beta 1 ) , fibronectin mRNA expression and protein synthesis , because antioxidants effectively inhibited HG induced PKC , NF kappaB , AP 1 activation , and TGF beta 1 and fibronectin expression in mesangial cells cultured under HG . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Effect of lovastatin on small GTP binding proteins and on TGF beta 1 and fibronectin expression . ^^^ We have also shown that lovastatin suppresses both control and high glucose ( HG ) induced TGF beta 1 and fibronectin mRNA expression and protein synthesis by rat mesangial cell ( RMC ) and that this down regulation by lovastatin is reversed by mevalonate . ^^^ To determine the isoprenoid and small GTP binding proteins involved in TGF beta 1 and fibronectin expression . ^^^ Suppression of TGF beta 1 and fibronectin expression by lovastatin was reversed effectively when GGPP was added alone . ^^^ Partial reversal of lovastatin effect on fibronectin and TGF beta 1 expression was found when FPP was added alone . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
RESULTS : Northern blot analysis revealed significantly increased steady state levels of TGF beta 1 and ECM proteins ( collagen types 1 , 3 , and 4 , and fibronectin ) by approximately twofold . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Pancreata were histopathologically examined at every 4 weeks , and the expression of fibrosis related factors such as transforming growth factor beta 1 ( TGF beta 1 ) , fibronectin ( FN ) , alpha smooth muscle actin ( alpha SMA ) , and type 3 collagen was analyzed . ^^^ In untreated WBN / Kob rats , chronic pancreatitis developed at 12 weeks and progressed with marked fibrosis at 16 weeks , and the expression of TGF beta 1 and FN peaked at 12 weeks . ^^^ However , in the TJ 10 treated rats , the rate of pancreatic fibrosis and the expression of TGF beta 1 , FN , alpha SMA , and type 3 collagen at 12 and 16 weeks decreased significantly compared to those in the untreated rats . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Exogenous TGF beta 0 1 ng / ml induced a dose dependent increase in FN protein ( 280+ / 45 % increase at TGF beta 1 ng / ml , P < 0 . 0001 ) , and FN mRNA levels ( 10 . 0+ / 1 . 8 fold at TGF beta 1 ng / ml ) . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
After unilateral ureteral obstruction , TGF beta 1 and CTGF messenger RNA ( mRNA ) expression in the obstructed kidney was coordinately upregulated from the early stage of interstitial fibrosis , followed by marked induction of fibronectin and alpha 1 ( 1 ) collagen mRNA expression . ^^^ In cultured normal rat kidney fibroblast ( NRK 49F ) cells , CTGF antisense ODN transfection significantly attenuated TGF beta 1 induced fibronectin and alpha 1 ( 1 ) collagen mRNA expression compared with control reverse ODNs . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
ANG 2 and TGF beta 1 increased fibronectin secretion , smooth muscle alpha actin synthesis , and formation of actin stress fibers and enhanced attachment of fibroblasts to a fibronectin matrix . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
High expression of collagen , fibronectin , tenascin , and alpha 2 integrin was detected in the TGF beta 1 expressing tumors by immunohistochemistry . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
On day 70 , the ECM composition was expanded by increased deposition of fibronectin , whereas connective tissue growth factor , fibroblast growth factor ( FGF ) 1 , and FGF 2 mRNA expression levels were elevated in addition to TGF beta 1 . ^^^ In vitro , TGF beta 1 stimulated collagen type 1 expression but not fibronectin expression in PSC , in contrast to FGF 2 , which stimulated both . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Cells maintained in suspension culture ( i . e . , over agarose underlays ) in growth factor free medium or treated with TGF beta 1 in suspension expressed relatively low levels of PAI 1 transcripts compared with the significant induction of PAI 1 mRNA evident in T 2 cells upon stimulation with TGF beta 1 during adhesion to a fibronectin coated substrate . ^^^ Attachment to fibronectin alone ( i . e . , in the absence of added growth factor ) was sufficient to initiate PAI 1 transcription , albeit at levels considerably lower than that induced by the combination of cell adhesion in the presence of TGF beta 1 . ^^^ PAI 1 mRNA synthesis in response to substrate attachment , like TGF beta 1 mediated induction in adherent cultures , also required MEK activity as fibronectin stimulated PAI 1 expression was effectively attenuated by the MEK inhibitor PD 98059 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
TGF beta 1 up regulates paxillin protein expression in malignant astrocytoma cells : requirement for a fibronectin substrate . ^^^ TGF beta 1 stimulation of primary astrocytes and serum starved U 251MG malignant astrocytoma cells attached to fibronectin induced a substantial increase in the levels of paxillin protein ( fivefold increase at 2 . 0 ng / ml ) in a dose and time dependent manner compared to the levels observed on plating onto fibronectin in the absence of stimulation . ^^^ The TGF beta 1 stimulated increase in paxillin levels required ligation of the fibronectin receptor , as it was not induced when the cells were plated onto vitronectin , collagen or laminin . ^^^ The increase in the pool of paxillin on TGF beta 1 stimulation of the fibronectin plated astrocytoma cells was associated with an increase in translation , but was not associated with an increase in the steady state levels of paxillin mRNA . ^^^ Stimulation with TGF beta 1 on a fibronectin substrate increased subsequent attachment and spreading of U 251MG cells onto fibronectin and , to a lesser extent , vitronectin , but not collagen . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
The expression of the three mRNAs of HSCs in vitro isolated from the experimental group ( 6 weeks ) was higher than those from normal liver . ( 3 ) The expression of FN , alpha 5 and beta 1 mRNAs increased in normal rat HSCs after treatment with transforming growth factor beta 1 ( TGF beta 1 ) for 2 hours . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
The degree of tubular lesion , the distribution of transforming growth factor beta ( TGF beta ) , alpha smooth muscle actin and fibronectin and the activated TGF beta 1 level were determined . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
When compared with normal rats , an elevated content of intraglomerular TGF beta 1 ( 44 . 25 + / 21 . 9 vs . 71 . 1 + / 23 . 4 ng / microgram DNA , p < 0 . 05 ) as well as fibronectin ( 2 . 62 + / 0 . 49 vs . 3 . 42 + / 0 . 62 ng / microgram DNA , p < 0 . 05 ) was observed . ^^^ In the diabetic rats , treatment with intraperitoneal proteases prevented the rise of intraglomerular TGF beta 1 content ( 34 . 9 + / 22 . 2 ng / microgram DNA , p < 0 . 01 ) and attenuated the rise of fibronectin ( 3 . 03 + / 1 . 12 ng / microgram DNA NS ) . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Immunoreactivities for TGF beta 1 and FN were found in islets of OLETF rats at 20 wk of age and were seen in acinar and interstitial cells at 50 wk of age . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Using unilateral ureteral obstruction ( UUO ) in rats , we analyzed gene expression of TGF beta 1 , CTGF , and fibronectin . ^^^ After UUO , CTGF mRNA expression in the obstructed kidney was significantly upregulated subsequent to TGF beta 1 , followed by marked induction of fibronectin mRNA . ^^^ With the use of a reporter assay , rat fibronectin promoter activity was increased by 2 . 5 fold with stimulation by TGF beta 1 , and this increase was abolished with antisense CTGF treatment . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
With this information , optimization of the triarylimidazole hit 8 gave the selective inhibitor 14 , which inhibits TGF beta 1 induced fibronectin mRNA formation while displaying no measurable cytotoxicity in the 48 h XTT assay . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Northern blot analysis and immunocytochemistry techniques were performed in order to detect the expression of alpha ( 5 ) beta ( 1 ) , FN mRNA and their relevant proteins in cultured rat pulmonary fibroblasts ( PFbs ) after administration of TGF beta 1 . ^^^ Changes in FN were similar to that of the integrin alpha ( 5 ) beta ( 1 ) . ( 2 ) Expression of alpha ( 5 ) beta ( 1 ) , FN mRNAs and their relevant proteins was increased in rat PFbs after TGF beta 1 administration . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Treatment with exogenous TGF beta 1 resulted in a failure to induce transcription from an artificial Smad dependent promoter and a failure to down regulate c myc , but resulted in an up regulation of AP 1 associated genes ( Fra 1 , JunB and fibronectin ) . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Moreover , we evaluated the production of interleukin 1 beta ( IL 1beta ) , transforming growth factor beta 1 ( TGF beta 1 ) , fibronectin , and prostaglandin E 2 ( PGE 2 ) by pure fibroblasts ( fourth passage ) . ^^^ Levels of IL 1beta , TGF beta 1 , and fibronectin in conditioned mediums ( CM ) of fibroblast cultures were measured by enzyme linked immunosorbent assay ( ELISA ) kit and PGE ( 2 ) by radioimmunoassay ( RIA ) kit . ^^^ In conclusion , BM fibroblasts from LCP and BCP presented a defective proliferative and confluence capacity , and this deficiency may be associated with the alteration of IL 1beta , TGF beta 1 , fibronectin , and PGE 2 production . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
We previously showed that type 2 IFN gamma reduces adhesiveness to fibronectin ( Fn ) whereas TGF beta 1 enhances cell attachment . ^^^ In contrast , IFN beta , like IFN gamma , abrogated U 937 adhesion to fibronectin and antagonized TGF beta 1 mediated cell attachment to Fn . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Similarly , these two kinds of therapies in SHR DM abolished the overexpression of renal TGF beta 1 by Western blot analysis and reduced the accumulation of collagen type 4 , laminin and fibronectin proteins by an immunochemical approach . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
In this study we used immunohistochemistry to investigate the presence of transforming growth factor beta 1 ( TGF beta 1 ) , insulin like growth factor 1 ( IGF 1 ) , interleukin 6 ( IL 6 ) , IL 6 receptor ( IL 6R ) and fibronectin in lumbar disc bioptic specimens from 30 patients with disc herniation ( protrusion type ) . ^^^ Chondrocytes of herniated discs stained positive for TGF beta 1 , IGF 1 , IL 6 and fibronectin . ^^^ In these sections no immunoreaction for TGF beta 1 , IL 6 , or IL 6R was found , while they expressed IGF 1 and fibronectin , but in lower quantities than herniated discs . ^^^ These results demonstrated the production of factors such as TGF beta 1 , IGF 1 , IL 6 , IL 6R and fibronectin at the site of lumbar disc herniation . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Using single and double immunostaining techniques , renal tissues were examined for TSP 1 protein , TGF beta 1 , platelet derived growth factor BB , extracellular matrix proteins , such as collagens and fibronectin , myofibroblast formation and macrophage influx . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
FGF 2 also induced intracellular fibronectin synthesis but not secretion , the latter of which was stimulated exclusively by TGF beta 1 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
METHODS : We measured serum glycated albumin , urine protein , glomerular TGF beta 1 expression and morphometry , and collagen 4 and fibronectin mRNA in db / m and db / db controls and in db / db mice treated for eight weeks with a synthetic compound that inhibits the condensation of glucose with albumin . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
All compounds inhibited TGF beta 1 induced fibronectin ( FN ) mRNA , indicating that FN synthesis is mediated in part via the p 38 MAPK pathway . ^^^ These data indicate that some matrix markers that are stimulated by TGF beta 1 are mediated via the p 38 MAPK pathway ( i . e . , FN ) , whereas others seem to be activated via ALK 5 signaling independent of the p 38 MAPK pathway ( i . e . , col Ialpha 1 ) . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Our results show that the TGF beta 1 intracellular Ca ( 2+ ) signal is responsible , in part , for stimulation of alpha 5 integrin expression , but not beta 1 integrin or FN expression . ^^^ TGF beta 1 increased both FN and beta 1 integrin protein production within 48 h , independent of nifedipine cotreatment . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
This study was performed to investigate the effects of transforming growth factor beta 1 ( TGF beta 1 ) latency associated peptide ( LAP ) and betaglycan on TGF beta 1 activity , and on the glucose induced overproduction of fibronectin in cultured human mesangial cells ( MCs ) . ^^^ Recombinant LAP and soluble betaglycan also inhibited a recombinant TGF beta 1 stimulated increase in fibronectin production in MCs . ^^^ These results indicate that recombinant LAP and soluble betaglycan suppress the glucose induced overproduction of fibronectin presumably via inhibition of TGF beta 1 activity in MCs . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Renal levels of TGF beta 1 and beta 2 , alpha actin , type 3 collagen , and fibronectin mRNA decreased in rats treated with anti TGF beta Ab . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Co incubation with neutralizing anti TGF beta 1 antibodies reduced ( P < 0 . 05 ) CsA induced expression of TbetaR 1 ( 1 . 0+ / 0 . 1 fold ) , TbetaR 2 ( 1 . 3+ / 0 . 1 fold ) and PAI 1 ( 1 . 3 fold ) , but not FN production ( 1 . 6 fold ) . ^^^ Whereas up regulation of PAI 1 is mediated by TGF beta 1 , up regulation of FN is at least in part either directly induced by CsA or mediated by factors other than TGF beta1 . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
In mesangial cells , high glucose potentiated the effect of low dose TGF beta 1 ( 0 . 2ng / ml ) on the following TGF beta responsive constructs : 3TP Lux ( containing AP 1 sites and PAI 1 promoter ) , SBE 4 Luc ( containing four tandem repeats of SBE sequence ) , and the fibronectin promoter . ^^^ Additionally , Smad 3 overexpression increased fibronectin promoter activity , an effect that was enhanced by high ambient glucose or treatment with TGF beta 1 ( 2ng / ml ) . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Corticosteroids are standard therapy in asthma and the presence of dexamethasone decreased wound induced release of TGF beta 1 and the expression of collagen 1 , fibronectin , and TbetaR 2 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
TGF beta 1 thus up regulates the synthesis of extracellular matrix components such as fibronectin and collagen 4 providing a basis for the restoration of epithelial coverage in the regenerating tubule . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
The combined therapy also synergistically inhibited the accumulation of interstitial matrix components , such as fibronectin and collagen 1 , and suppressed renal expression of transforming growth factor beta 1 ( TGF beta 1 ) and its type 1 receptor . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Furthermore , MKK 3 deficiency resulted in a selective disruption of TGF beta 1 stimulated up regulation of pro alpha 1 ( 1 ) collagen expression but not TGF beta 1 induction of fibronectin and PAI 1 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Given that interaction between the extracellular matrix protein fibronectin and alpha5beta1 integrin on the cell surface is principally responsible for osteoblast substrate adhesion , we examined here whether the TGF beta 1 stimulated Ca2+ signal is involved in this pathway . ^^^ Our results show that , in primary human osteoblasts , the TGF beta 1 induced intracellular Ca2+ signal is responsible , in part , for the stimulation of expression of alpha 5 integrin , but not of beta 1 integrin or fibronectin . ^^^ TGF beta 1 treatment increased both fibronectin and beta 1 integrin protein production within 48 h , in a manner unaffected by co treatment with nifedipine . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Protein levels of transforming growth factor ( TGF ) beta 1 , fibronectin , PGE ( 2 ) , and TGF beta 1 mRNA were quantified . ^^^ CSE also inhibited fibronectin and TGF beta 1 production in low density cultures but stimulated fibronectin production in high density cultures . ^^^ These results suggest that CSE induced inhibition of low density fibroblast contraction is due to inhibition of fibronectin production , whereas CSE ' s stimulatory effect on high density cells is the result of increased release of TGF beta 1 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Ureteral obstruction induced significant increases in alpha 2 ( 1 ) and alpha 1 ( 4 ) collagen , fibronectin , and TGF beta 1 mRNA levels , as well as in total kidney collagen but changes were similar in Eng ( + / ) and Eng ( + / + ) mouse kidneys . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
The present study was designed to characterize the morphology of the frontonasal ( FN ) suture of the rat at different developmental stages and to investigate the presence and temporal spatial expression of transforming growth factor beta 1 ( Tgf beta 1 ) , Tgf beta 2 , Tgf beta 3 and Msx 2 mRNA within these structures . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
METHODS : Using in situ hybridization and immunohistochemistry to detect Smad 2 mRNA expression and TGF beta 1 , collagen 4 , fibronectin expression in renal biopsies from 61 cases with a spectrum of glomerulonephritis including IgA nephropathy ( 40 cases ) , membranous glomerulonephritis ( 10 cases ) and sclerosing glomerulonephritis ( 11 cases ) , compared with 11 cases of glomerular mild lesion with image analysis system . ^^^ The expressions of glomerular TGF beta 1 and Smad 2 mRNA positively correlated with collagen 4 and fibronectin deposition in the glomeruli . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Therefore , we examined adhesion of bone marrow derived mast cells cultured with and without TGF beta 1 to laminin 1 , fibronectin , and vitronectin along with expression of integrins likely to regulate this adhesion . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Immunohistochemical analysis of cardiac biopsy specimens showed strong fibronectin staining in the extracellular matrix and staining for phosphorylated Smad 2 ( activation of the TGF beta 1 signaling pathway ) in cell nuclei . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
However , whereas fibronectin , bFGF , hepatocyte growth factor , and TGF beta 1 significantly augmented migration of mouse myoblasts , TNF alpha did not , nor did PDGF BB or IGF 1 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
AII , TGF beta 1 , fibronectin and type 4 collagen in the culture media were measured by ELISA . ^^^ The glucose induced increases in the production of TGF beta 1 , fibronectin and type 4 collagen were partially , but significantly restored ( P < 0 . 01 ) by CC , while GFX totally abolished these effects of glucose . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Activation of the mitogen activated protein kinase ( MAPK ) transforming growth factor beta 1 ( TGF beta 1 ) fibronectin pathway in kidney , responsible for glomerular dysfunction , was markedly blunted by SP treatment in a dose dependent manner . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
TGF beta 1 rapidly reduced hepatocarcinoma cells adhesion on to fibronectin and stimulated FAK dephosphorylation ] . ^^^ Our results showed that TGF beta 1 could rapidly attenuated cell adhesion onto Fn without changing the expression of alpha 5 beta 1 integrin , and at the meantime dephosphorylated FAK . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Effect of amniotic membrane on expressions of TGF beta 1 , collagens 1 , 3 and fibronectin in rabbit corneal healing after photorefractive keratectomy ] . ^^^ OBJECTIVE : To observed the effect of amniotic membrane transplantation ( AMT ) on expressions of transforming growth factor beta 1 ( TGF beta 1 ) , collagens 1 , 3 and fibronectin ( FN ) in rabbit corneal healing after photorefractive keratectomy ( PRK ) , and investigate the anti scarring formation mechanism of AMT . ^^^ The expressions of TGF beta 1 , collagens 1 , 3 and FN were studied by immunohistochemistry at 4 weeks after operation . ^^^ CONCLUSION : The expressions of TGF beta 1 , collagens 3 and FN can be suppressed by amniotic membrane after PRK . ^^^ These data suggest that part of the anti scarring effect of AMT may be mediated through the suppression of TGF beta 1 , collagens 3 and FN expression . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
It significantly suppressed renal malondialdehyde ( MDA ) , microalbuminuria , glomerular hypertrophy , mesangial expansion , and the upregulation of renal TGF beta 1 , fibronectin , and collagen in STZR without significantly affecting plasma glucose . ^^^ Both 30 mM of glucose and 100 uM of H ( 2 ) O ( 2 ) significantly increased TGF beta 1 and fibronectin protein secretion by mesangial cells . ^^^ LAB at 10 micro g / ml inhibited high glucose and H ( 2 ) O ( 2 ) induced TGF beta 1 and fibronectin secretion . ^^^ The in vitro data of this study show that LAB inhibits ROS generation leading to PKC activation and TGF beta 1 and fibronectin upregulation in mesangial cells cultured under high glucose conditions . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
TGF beta 1 up regulates expression of integrins and fibronectin , an effect that is associated with autophosphorylation / activation of FAK . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
In the same way , expressions of fibronectin , collagen type 3 , 5 and transforming growth factor beta 1 ( TGF beta 1 ) were measured . ^^^ Cultured HPMCs coexpressed cytokeratin and vimentin , and synthesized fibronectin , TGF beta 1 and collagen types 3 ( instead of type 5 ) . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Evidence for a novel TGF beta 1 independent mechanism of fibronectin production in mesangial cells overexpressing glucose transporters . ^^^ Our data indicate that increased glucose uptake and metabolism induce PKC dependent AP 1 activation that is sufficient for enhanced fibronectin production , but not for increased TGF beta 1 expression . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Glucose induced fibronectin and collagen type 3 expression in renal fibroblasts can occur independent of TGF beta 1 . ^^^ METHODS : Human renal fibroblasts ( TK 173 ) were used to examine the effects of high glucose and TGF beta 1 on fibronectin and collagen type 3 expression . ^^^ RESULTS : Fibronectin secretion by renal fibroblasts was increased upon exposure to high glucose , but with delayed kinetics compared to TGF beta 1 induced fibronectin . ^^^ Importantly , fibroblasts expressing a dominant negative TGF beta type 2 receptor were defective in TGF beta 1 induced fibronectin production , whereas glucose induced fibronectin and collagen type 3 were unaffected . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
After 40 days mouse survival , albuminuria , matrix accumulation , inflammatory cell infiltration , immunoglobulin G ( IgG ) deposition , expression of transforming growth factor beta 1 ( TGF beta 1 ) , fibronectin and plasminogen activator inhibitor 1 ( PAI 1 ) mRNA and protein , anti DNA antibody titer , inducible nitric oxide synthase ( iNOS ) mRNA expression , blood amino acid levels , blood urea nitrogen ( BUN ) concentrations and blood and urinary NOx ( nitrite + nitrate ) levels were assessed . ^^^ L Arginine administration increased albuminuria , renal matrix accumulation , TGF beta 1 , fibronectin , PAI 1 , blood L arginine , L citrulline , BUN and blood and urine NOx levels , while protein restriction reduced these parameters . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
When rat mesangial cells were cultured with an amino acid mixture designed to replicate the composition in plasma after protein feeding , production of mRNA ( Northern blot analysis ) and / or protein ( ELISA or Western blot analysis ) for transforming growth factor beta 1 ( TGF beta 1 ) , fibronectin , thrombospondin 1 ( TSP 1 ) , and collagen 4 were enhanced in a manner comparable to a culture with high glucose ( 30 . 5 mM ) . ^^^ The TSP 1 antagonist LSKL peptide reduced bioactive TGF beta and fibronectin , indicating the dependence of TGF beta 1 activation on TSP 1 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Furthermore , the ECM substrates fibronectin and vitronectin , but not laminin , also promoted microglial activation and increased expression of the alpha ( 4 ) beta ( 1 ) , alpha ( 5 ) beta ( 1 ) and Mac 1 integrins , but significantly , the influence of fibronectin and vitronectin was not diminished by TGF beta 1 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
TN C was then compared with fibronectin ( FN ) , laminin ( LN ) , basic fibroblast growth factor ( b FGF ) and transforming growth factor beta 1 ( TGF beta 1 ) . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
MAIN OUTCOME MEASURE ( S ) : Multiplex reverse transcriptase polymerase chain reaction ( RT / PCR ) of type 3 collagen , fibronectin , TGF beta 1 , and beta actin was performed , with analysis of PCR amplified products performed by densimetric analysis of gel bands using the National Institutes of Health Image analysis program . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
RESULTS : TGF beta 1 had no effect on RIE 1 adhesion to collagen types 1 and 4 , fibronectin , and laminin , or invasion through collagen types 1 and 4 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
METHODS : The expression of proliferative cell nuclear antigen ( PCNA ) , epithelial cell growth factor ( EGF ) , alpha smooth muscle actin ( alpha SMA ) , fibronectin ( FN ) , collagen type 3 and 4 , transforming growth factor beta 1 ( TGF beta 1 ) and connective tissue growth factor in renal biopsy samples of patients with MD ATN ( 4 patients ) and A ATN ( 5 patients ) were compared with immunohistochemical SP method . ^^^ RESULTS : ( 1 ) The PCNA positive rate of renal tubular epithelial cell ( RTEC ) and EGF expression in the MD ATN group were significantly lower than those in the A ATN group respectively ( P < 0 . 01 ) ; ( 2 ) Renal tubular mesenchymal alpha SMA level increased in both groups with no significant difference ; ( 3 ) TGF beta 1 positive cell infiltration and obvious CTGF expression revealed in renal mesenchyma of both groups with insignificant difference ; ( 4 ) FN , collagen 3 and 4 levels were significantly higher in the MD ATN group than that in the A ATN group ( P < 0 . 05 ) . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Glutamine : fructose 6 phosphate aminotransferase enzyme activity is necessary for the induction of TGF beta 1 and fibronectin expression in mesangial cells . ^^^ Production of TGF beta 1 and fibronectin protein was examined by ELISA . ^^^ An increased concentration of wild type GFAT in mesangial cells enhanced both TGF beta 1 and fibronectin production 1 . 5 fold , while mesangial cells transfected with the mutated GFAT constructs showed no effect . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Analysis included systolic blood pressure , a glomerular histologic matrix score , and the glomerular mRNA and protein expression of the key fibrogen TGF beta 1 , the matrix protein fibronectin , and the protease inhibitor plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^ Glomerular matrix accumulation , fibronectin and PAI 1 mRNA and protein expression closely followed the expression of TGF beta 1 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
METHODS : We investigated the effects of emodin on the expression of PKC alpha , TGF beta 1 , fibronectin , and collagen type 1 in HPMC , and its effects on HPMC proliferation under physiologic ( 5 mmol ) or high ( 30 mmol ) glucose concentrations . ^^^ Induction of TGF beta 1 synthesis by 30 mmol D glucose was associated with induction of PKC alpha , phosphorylation of cAMP responsive element binding protein ( CREB ) and activating transcription factor 1 ( ATF 1 ) , and increased fibronectin and type 1 collagen translation . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Collagens 1 , 3 , 4 , and 5 and fibronectin were increased in explants treated with pMX L s 223 , 225 TGF beta 1 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
We assessed the content of fibronectin ( FN ) and transforming growth factor beta 1 ( TGF beta 1 ) as well as the activities of collagenase and cathepsin B and L in renal artery walls of chronically rejected human renal allografts . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
We previously showed that the high glucose mediated fibronectin and laminin synthesis in MES cells is mediated by the HBP and that GlcN is more potent than glucose in inducing TGF beta 1 promoter luciferase activity . ^^^ The presence of anti TGF beta 1 antibodies ( 20 microg / ml ) blocks both TGF beta 1 and GlcN induced fibronectin synthesis . ^^^ TGF beta 1 increased ECM levels via PKA ( laminin and fibronectin ) and PKC ( fibronectin ) pathways . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
BACKGROUND : To determine the plasma concentrations of placental growth factor ( PLGF ) , vascular endothelial growth factor ( VEGF ) , transforming growth factor beta 1 ( TGF beta 1 ) , soluble tumor necrosis factor alpha receptor ( sTNFp 55 ) , interleukin 2 receptor ( IL 2R ) , and interleukins 6 and 10 ( IL 6 , IL 10 ) in normotensive and preeclamptic women , and to evaluate the correlations between these cytokines and the diastolic blood pressure and fibronectin levels . ^^^ Peripheral venous blood samples were obtained and plasma levels of PLGF , VEGF , TGF beta 1 , sTNFp 55 , IL 2R , IL 6 and IL 10 were measured by an enzyme linked immunoassay and fibronectin by a radial immundiffusion technic . ^^^ The plasma levels of PLGF and VEGF significantly decreased , whereas TGF beta 1 , sTNFp 55 , IL 2R , IL 6 and IL 10 levels significantly increased with the increments in diastolic blood pressure and fibronectin levels ( p < 0 . 001 ) . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Fn and PAI 1 significantly increased compared with control with 5 ng / ml TGF beta 1 stimulated at 24 hours and 48 hours ( P < 0 . 05 ) ; 3 . ^^^ The different concentration of huangqi decreased FN and PAI 1 expression compared with TGF beta 1 group , especially 1 mg / ml huangqi at 24 hours ( P < 0 . 05 ) . 4 . ^^^ FN , PAI 1 and bFGF mRNA expression were higher in 12 hours after stimulation with TGF beta 1 compared with control . ^^^ FN , PAI 1 and bFGF mRNA expression were lower in 12 hours after stimulation with different concentration of huangqi ( 100 micrograms / ml and 1 mg / ml ) than those in the TGF beta 1 group . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
At various time points , corneas were processed for histology and stained for nuclei , f actin , ED A fibronectin , alpha smooth muscle actin , TGF beta 1 , TGF beta 2 , TGF beta receptor 2 , and CTGF . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Immunohistochemistry was used to examine the expression of transforming growth factor beta 1 ( TGF beta 1 ) , type 4 collagen and fibronectin in the glomeruli . ^^^ The glomerular expressions of TGF beta 1 , type 4 collagen and fibronectin were significantly up regulated in the model group in comparison with those in the sham operation group ( all P < 0 . 01 ) . ^^^ The glomerular protein expressions of TGF beta 1 , type 4 collagen and fibronectin were significantly weaker in the fluvastatin treatment group as compared with the model group ( all P < 0 . 01 ) . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Increased expression levels of TGF beta 1 , collagen 3 and fibronectin mRNA in 5 were reduced in Q 20 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
RESULTS : Glomerular expression of CTGF and TGF beta 1 mRNA was coordinately upregulated as early as day 3 , followed by fibronectin induction and mesangial matrix accumulation . ^^^ Chronic aspirin treatment in diabetic rats significantly attenuated mesangial expansion , and effectively suppressed CTGF induction , as well as inhibiting the upregulation of TGF beta 1 and fibronectin expression . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Immunohistochemistry with image analysis was performed to detect the expression of BrdU , alpha SMA , decorin , TGF beta 1 , FN and ColIV in diseased glomeruli . ^^^ TGF beta 1 , FN , ColIV expressions in diseased glomeruli of rats with ATS nephritis were decreased significantly at day 4 ( TGF beta 1 , P < 0 . 05 ) and day 2 ( FN and ColIV , P < 0 . 01 ) respectively , compared to uninjected kidneys . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
By FACS and Western blot analysis , we observed that TGF beta 1 enhanced the expression of alpha5beta1 integrin obviously , and subsequently stimulated cell adhesion onto fibronectin ( Fn ) . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
At 0 , 24 , and 72 h after wounding , changes in mRNA expression of transforming growth factor beta 1 ( TGF Beta 1 ) , b FGF , HGF , VEGF , and FN were semiquantified by reverse transcription polymerase chain reaction . ^^^ For RPMC , TGF Beta 1 , HGF , b FGF , and FN mRNA gradually increased up to 72 h postwounding to 1 . 5 fold , 1 . 6 fold , 1 . 3 fold , and 2 . 1 fold of the control levels , respectively . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Peroxisome proliferator activated receptor gamma ligands inhibit TGF beta 1 induced fibronectin expression in glomerular mesangial cells . ^^^ TGF beta 1 enhanced fibronectin mRNA expression , and this enhancement was abrogated by pretreatment with pioglitazone . ^^^ PPAR gamma overexpression inhibited TGF beta 1 induced fibronectin expression as well as the activation of AP 1 . 15 Deoxy Delta ( 12 , 14 ) prostaglandin J ( 2 ) ( 15d PGJ ( 2 ) ) , a natural PPAR gamma ligand , also inhibited TGF beta 1 induced fibronectin expression by suppressing AP 1 activation by TGF beta 1 . 15d PGJ ( 2 ) inhibited the TGF beta 1 induced MAPK activation . ^^^ In conclusion , pioglitazone inhibits TGF beta 1 induced fibronectin expression by inhibiting AP 1 activation dependent on PPAR gamma , while 15d PGJ ( 2 ) acts through a dual mechanism independent of and dependent on PPAR gamma activation in mouse mesangial cells . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Baseline active TGF beta 1 levels were increased in both types of ADO ( 60 % in ADO 1 [ P = 0 . 006 ] ; 46 % in ADO 2 [ P = 0 . 001 ] , respectively ) , whereas fibronectin levels were decreased in both ( ADO 1 58 % and ADO 2 63 % of normal , respectively [ P = 0 . 012 and P = 0 . 001 ] ) . ^^^ In the murine models , active TGF beta 1 was significantly decreased in the tl and ia rat , whereas fibronectin levels were decreased in the mi mouse , however , increased in the ia rat . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
The other half was frozen and reverse transcriptase polymerase chain reaction was performed to measure steady state levels of epidermal growth factor , transforming growth factor ( TGF ) alpha , TGF beta 1 , TGF beta 2 , TGF beta 3 , keratinocyte growth factor , vascular endothelial growth factor , insulin like growth factor , platelet derived growth factor , collagen type 1 , integrin and fibronectin transcript expression . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Fibronectin ( FN ) and TGF beta 1 levels were assayed in the supernatant from cultured HK 2 cells by ELISA . ^^^ Fibronectin ( FN ) and TGF beta 1 levels were assayed in the supernatant from cultured HK 2 cells by ELISA . ^^^ RESULTS : After stimulation by 2 . 5 mg / L AA 1 , HK 2 cells secreted TGF beta 1 at hour 12 and FN at hour 36 . ^^^ Anti TGF beta 1 neutralizing antibody ( 5 mg / L ) could suppress AA 1 induced apoptosis by 63 . 7 % ( P < 0 . 001 ) , and it blocked AA 1 induced FN secretion by 50 . 2 % ( P < 0 . 001 ) . ^^^ In contrast , Anti TGF beta 1 neutralizing antibody had no effect on AL 1 induced apoptosis and FN secretion , even though AL 1 ( 5 mg / L ) had similar effect on these events compared to AA 1 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
HIV infected lymphocytes regulate fibronectin synthesis by TGF beta 1 secretion . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
EGF , PDGF , TGF beta 1 , fibronectin , and vitamin A were quantified by means of ELISA and HPLC . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Furthermore , transfection of dominant negative mutants of p38alpha and p38delta resulted in marked inhibition of TGF beta 1 induced VEGF 164 expression but not VEGF 188 , and treatment with recombinant mouse VEGF 164 increased collagen and fibronectin mRNA expression in mesangial cells . ^^^ Further , VEGF 164 stimulates collagen and fibronectin expression in mesangial cells and thus in turn enhances TGF beta 1 induced extracellular matrix and may play an important role in progressive glomerular fibrosis . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
TGF beta 1 and GnRHa increased ERK1 / 2 nuclear accumulation and inversely regulated the expression of c fos and c jun and that of fibronectin in a cell specific manner . ^^^ Pretreatment with U 0126 , a MEK1 / 2 inhibitor , blocked basal , as well as GnRHa and TGF beta 1 induced pERK1 / 2 ; however , it differentially affected c fos , c jun , and fibronectin expression . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
The expression of collagen 4 , fibronectin ( FN ) , laminin ( LN ) , transforming growth factor beta 1 ( TGF beta 1 ) and tumor necrosis factor alpha ( TNF alpha ) were detected by using immunohistochemistry or in situ hybridization in fibrous capsules around 40 hepatic hydatid cysts and 15 splenic hydatid cysts including adjacent parenchyma . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
In vitro effects of flunisolide on MMP 9 , TIMP 1 , fibronectin , TGF beta 1 release and apoptosis in sputum cells freshly isolated from mild to moderate asthmatics . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Hyperglycemia for 24 hr produced significantly increased fibronectin , slightly increased TGF beta 1 , and unchanged TGF beta 2 or TGF beta 3 , by immunohistochemistry . ^^^ Hyperglycemia increased fibronectin protein and mRNA expression in embryonic hearts after 24 hr , and pericardial injection of TGF beta 1 also increased fibronectin mRNA in the embryonic heart . ^^^ It is proposed that TGF beta 1 and fibronectin may play a role in diabetes induced cardiac dysmorphogenesis . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
At various time points , corneas were processed for histology and stained for nuclei ; F actin ; ED A fibronectin ; alpha smooth muscle actin ; TGF beta 1 , beta 2 , and beta receptor 2 ; and connective tissue growth factor ( CTGF ) . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
In week 20 , the following parameters were determined : blood lymphocyte number , kidney function , both glomerular and tubulointerstitial histologic matrix accumulation , protein expression of transforming growth factor beta 1 ( TGF beta 1 ) , fibronectin , and plasminogen activator inhibitor 1 ( PAI 1 ) as well as infiltration with macrophages and lymphocytes . ^^^ In comparison to the untreated chronic progressive glomerulosclerosis animals , the lymphocyte depletion achieved significantly limited the progression of the disease , as shown by lowered proteinuria , tubulointerstitial matrix expansion , and TGF beta 1 , fibronectin , and PAI 1 expression , as well as improved renal function . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
In culture , Smad 3 deficient fibroblasts expressed reduced fibronectin in response to TGF beta 1 compared to wild type cells . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Using Masson stain and light microscope , the authors examined the rats ' hepatic tissues and counted the hepatic fiber components , then examined and counted TGF beta 1 , alpha SMA , FN , Type 1 , 3 collagen by means of immunohistochemical technique . ^^^ RESULTS : The levels of FN , LN , Type 1 and 3 collagen , TGF beta 1 , and alpha SMA of the CCl 4 damaged rats increased ( P < 0 . 01 ) . ^^^ After 3 weeks of high dose `` anti hepatic fibrosis 268 ' ' treatment , the levels of TGF beta 1 , alpha SMA , FN , LN , Type 1 and 3 collagen decreased ( P < 0 . 01 ) and the degree hepatic fibrosis took a favorable turn significantly ( P < 0 . 05 ) as compared with the model control . ^^^ In the rats of the low dose group , the levels of TGF beta 1 , alpha SMA , FN , Type 3 collagen significantly decreased ( P < 0 . 05 ) , the levels of LN , Type 1 collagen were not different from the model control ; The hepatic fibrosis improved to a certain extent ( P < 0 . 05 ) . ^^^ CONCLUSION : The mechanism of reversing hepatic fibrosis by `` anti hepatic fibrosis 268 ' ' in this experiment is that the medicine regulates TGF beta 1 and further affects alpha SMA , thus resulting in the decline of FN , Type 1 , 3 collagen levels in liver extracellular matrix . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
However , it is not known whether they are also involved in the homeostasis of intracellular fibronectin content via upregulation of protein kinase C ( PKC ) , extracellular signal regulated kinase ( ERK ) , and transforming growth factor beta 1 ( TGF beta 1 ) . ^^^ To determine this , we have studied the effect of multiple amines on fibronectin , TGF beta 1 , ERK , and PKC levels in mesangial cells under high glucose conditions . ^^^ However , levels of fibronectin , TGF beta 1 , ERK , and PKC were unaffected by either putrescine or spermidine . ^^^ A decrease in fibronectin secretion was accompanied by decreases in TGF beta 1 and ERK . ^^^ Such cumulative results lead us to hypothesize that agmatine reduces high glucose induced fibronectin secretion via several pathways including ERK TGF beta 1 fibronectin and spermine , via a decrease in TGF beta 1 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
The brains were perfusion fixed 14 days later and immunohistochemistry was performed for TGF beta 1 , beta 2 and beta 3 , p44 / 42 mitogen activated protein ( MAP ) kinases , and the extracellular matrix proteins laminin , vitronectin and fibronectin . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
The addition of exogenous TGF beta 1 ( 1 ng / ml ) for 8 h increased FN mRNA by 2 . 7 + / 1 . 1 fold . ^^^ Both the TGF beta 1 and high glucose induced FN mRNA increases were inhibited by SB 203580 and PD 98059 . ^^^ Dominant negative Smad 4 did not affect the FN mRNA increase induced by TGF beta 1 and high glucose . ^^^ The FN synthesis induced by high glucose and TGF beta 1 was not affected by the Smads pathway and was not due to increased osmolarity . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
An adhesion assay revealed that treatment with TGF beta 1 , HGF , and RA induced a marked reduction of cell attachment to fibronectin ( FN ) coated surfaces , whereas there was no change with bFGF and EGF . ^^^ These findings suggest that TGF beta 1 , HGF , and RA may regulate pulpal cell adhesion to FN coated surfaces and that this effect is mediated by TN . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Glomerular mRNA and production of fibronectin , plasminogen activator inhibitor 1 ( PAI 1 ) , TGF beta 1 , and p Smad 2 protein were also reduced . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Term placental fibroblasts were isolated and cultured under hypoxia ( 3 % O ( 2 ) ) or in the presence of TGF beta 1 , and the expression of fibronectin , collagen 1 , and collagen 4 was examined using immunohistochemistry , ELISA of cell monolayers with associated ECM , and real time RT PCR . ^^^ Both TGF beta 1 and hypoxia increased fibronectin , collagen 1 , and collagen 4 protein and mRNA in placental fibroblasts . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
METHODS : We evaluated the time course of TGF beta 1 fibronectin , Smad 2 and Smad 3 protein expression and Smad 3 activation in glomeruli from spontaneously diabetic Otsuka Long Evans Tokushima Fatty ( OLETF ) rats , using immunohistochemistry and Western blot analysis . ^^^ No differences were found in TGF beta 1 fibronectin , Smad 2 and Smad 3 protein expression and Smad 3 activation in glomeruli between the two strains at 12 weeks of age when OLETF rats were not diabetic . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
METHOD : Fibronectin ( FN ) and collagen type 4 ( Col 4 ) of extracellular matrix were detected by enzyme linked immunosorbent assay ; the expression of TGF beta 1 mRNA were measured by semi quantitative reverse transcription polymerase chain reaction . ^^^ FN , Col 4 and TGF beta 1 mRNA were inhibited by Shenluotong significantly . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
High levels of glucose also increase EDB ( + ) FN expression in endothelial cells ( ECs ) via transforming growth factor beta 1 ( TGF beta 1 ) and endothelin 1 ( ET 1 ) . ^^^ Using human macro and microvascular ECs , we show that high levels of glucose , TGF beta 1 , and ET 1 increase the EDB ( + ) FN expression via SGK 1 alteration at the mRNA , protein , and activity levels . ^^^ Inhibition of TGF beta 1 and ET 1 prevented glucose induced SGK 1 activation and the EDB ( + ) FN expression . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
This went along with significant reductions in proteinuria , glomerular matrix accumulation , expression of TGF beta 1 , fibronectin , and PAI 1 , macrophage infiltration and fibrinogen deposition as compared to the untreated anti thy 1 animals . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
In these co cultures , TGF beta 1 treatment resulted in significantly increased mRNA levels of type 1 collagen and fibronectin in the TFB . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
ET 1 , ET 3 , transforming growth factor beta 1 ( TGF beta 1 ) and angiotensinogen mRNA levels were measured to elucidate genes involved in FN expression . ^^^ RESULTS : Our results show that diabetes leads to upregulation of FN , PAI 1 , ET 1 , ET 3 , TGF beta 1 and angiotensinogen mRNA levels in association with increased medial thickness . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
However , AM beginning at 10 ( 8 ) M alleviated the action of TGF beta 1 stimulated cellular collagen synthesis and secretion of fibronectin into cell culture supernatant . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
TGF beta 1 increased dichlorofluorescein sensitive cellular ROS , phosphorylated Smad 2 , p 38 MAPK , extracellular signal regulated kinases ( ERK ) 1 / 2 , alpha smooth muscle actin ( alpha SMA ) expression , and fibronectin secretion and decreased E cadherin expression . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Aminoguanidine and vitamin E prevented AGE accumulation and induction of protein and mRNA for fibrosis markers [ transforming growth factor beta 1 ( TGF beta 1 ) , fibronectin , and collagen 4 ] . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
The secretion level of fibronectin ( FN ) and TGF beta 1 in HK 2 cells were assayed by ELISA . ^^^ AL 1 could stimulate the secretion of FN and TGF beta 1 . ^^^ The effects of AL 1 on apoptosis , secretion of FN and TGF beta 1 were all weaker than AA 1 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
The concentrations of EGF , TGF beta 1 , PDGF AB , FGF , HGF , fibronectin , vitamin A and vitamin E in these samples were evaluated with ELISA and HPLC . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
The matrix metalloproteinases ( MMP 1 and MMP 8 ) , fibronectin , OPN , TGF beta 1 proteins and the mRNAs for fibronectin , TGF beta 1 , and OPN were significantly elevated in the infarct area as compared to the remote area and the non infarcted hearts . ^^^ Moreover , OPN , fibronectin and TGF beta 1 protein and gene expression are elevated after ischemia and reperfusion in the ex vivo working hemoperfused porcine heart model . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Angiotensin 2 mediates high glucose induced TGF beta 1 and fibronectin upregulation in HPMC through reactive oxygen species . ^^^ METHODS : In cultured HPMC , the expression of mRNAs for angiotensinogen , angiotensin converting enzyme ( ACE ) , Ang 2 type 1 receptor ( AT 1 ) , and TGF beta 1 was evaluated by real time polymerase chain reaction ; ACE , AT 1 , and fibronectin proteins by Western blot analysis ; and Ang 1 , Ang 2 , and TGF beta 1 proteins by ELISA . ^^^ Ang 2 increased TGF beta 1 and fibronectin protein expression and DCF sensitive cellular ROS . ^^^ Both losartan and captopril inhibited high glucose induced upregulation of TGF beta 1 and fibronectin expression in HPMC in a dose dependent manner . ^^^ Antioxidant catalase and NADPH oxidase inhibitor diphenyleneiodinium effectively inhibited Ang 2 induced TGF beta 1 and fibronectin protein expression . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
TGF beta 1 mediated activations of c Src and Rac 1 modulate levels of cyclins and p 27 ( Kip 1 ) CDK inhibitor in hepatoma cells replated on fibronectin . ^^^ We found that basal and TGF beta 1 mediated activation of c Src and Rac 1 , expression of cyclins E and A , and suppression of p27Kip1 were significant in cells replated on fibronectin , but not in cells on collagen 1 , indicating a different integrin mediated cellular response to TGF beta 1 treatment . ^^^ Studies using pharmacological inhibitors or transient transfections revealed that the preferential TGF beta 1 effects in cells on fibronectin required c Src family kinase activity . ^^^ These observations suggest that a specific cross talk between TGF beta 1 and fibronectin binding integrin signal pathways leads to the activation of c Src / Rac1 / actin organization , leading to changes in cell cycle regulator levels in hepatoma cells . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
DNA synthesis ( thymidine incorporation ) , intracellular Ca ( 2+ ) mobilization ( fura 2 fluorimetry ) , fibronectin secretion [ enzyme linked immunosorbent assay ( ELISA ) , immunoblotting ] , monocyte chemoattractant protein 1 ( MCP 1 ) secretion ( ELISA ) , and transforming growth factor beta 1 ( TGF beta 1 ) secretion ( ELISA ) were measured . ^^^ RESULTS : Thrombin enhanced DNA synthesis , fibronectin secretion , MCP 1 secretion , and TGF beta 1 secretion in a concentration dependent manner . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
While TGF beta 1 treatment in isolated pancreatic stellate cells ( PSCs ) stimulated the expression of alpha SMA and fibronectin , PSCs transfected with TGF beta dnRII showed attenuation of the ECM components . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Transforming growth factor beta 1 ( TGF beta 1 ) regulates ATDC 5 chondrogenic differentiation and fibronectin isoform expression . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
TGF beta 1 causes augmented extracellular matrix protein deposition ( collagen types 1 , 4 , 5 , and 6 ; fibronectin , and laminin ) at the glomerular level , thus inducing mesangial expansion and glomerular basement membrane thickening . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
In week 16 , analysis included effects on systolic blood pressure , proteinuria , kidney function , glomerular and tubulointerstitial matrix protein accumulation , expression of transforming growth factor beta 1 ( TGF beta 1 ) , fibronectin and plasminogen activator inhibitor type 1 ( PAI 1 ) , macrophage infiltration , cell proliferation , basal and nitric oxide stimulated cGMP production as well as tubulointerstitial mRNA expression of alpha 1 and beta 1 soluble guanylate cyclase . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
These findings were corroborated by studies using siRNAs to Smad 2 and 3 where siRNA to Smad 2 but not to Smad 3 inhibited the TGF beta 1 induction of fibronectin synthesis . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Immunoreactive TGF beta 1 , connective tissue growth factor , HGF receptor , c Met , and fibronectin all increased in remnant ( 2 . 85 + / 0 . 51 , 3 . 83 + / 0 . 55 , 2 . 56 + / 0 . 31 , and 2 . 80 + / 0 . 39 fold sham respectively , n = 4 8 , p < 0 . 05 ) , and SC 58236 blunted the increases ( to 1 . 45 + / 0 . 34 , 1 . 85 + / 0 . 13 , 1 . 75 + / 0 . 30 , and 1 . 60 + / 0 . 32 fold sham ) . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
RT PCR was done to assess expression of collagen 1 and 3 , fibronectin , MCP 1 , TGF beta 1 and bFGF . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
The effects on such indexes as systolic blood pressure ( SBP ) , left ventricular mass ( LVM ) , left ventricular mass index ( LVMI ) , content of myocardial collagen ( MC ) in left ventricle , extracellular matrix fibronectin ( FN ) , laminin ( LN ) , cardiac fibroblast ( cFb ) and transforming growth factor beta 1 ( TGF beta 1 ) were determined after 12 weeks of treatment . ^^^ RESULTS : SBP had no marked pressure depressive effect , but had the effect similar to that of benazepril in reducing the level of LVM , LVMI and content of MC ( P < 0 . 05 ) , as well as the content of LN , FN in myocardium , cFb count and TGF beta 1 expression ( P < 0 . 05 ) . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Kidney expression of RhoA , TGF beta 1 , and fibronectin in human IgA nephropathy . ^^^ Although a close relationship between increased renal tissue levels of TGF beta 1 and fibronectin has been reported in IgA nephropathy , no data are available on renal tissue expression of Rho proteins . ^^^ METHODS : This study was designed to assess in IgA nephropathy patients the kidney tissue immunohistochemical expression of RhoA , TGF beta 1 , and fibronectin , and the rate of immunoreactivity for each antigen by image analysis . ^^^ RESULTS : An increase in RhoA , TGF beta 1 , and fibronectin expression was detected in tubulointerstitium and in glomeruli of IgA nephropathy compared to normal kidneys ; in particular , RhoA was found also in proximal tubules , unlike control kidneys and mainly at the cell boundary level , which is in keeping with its activated form . ^^^ The image analysis confirmed that the kidney tissue levels of RhoA , TGF beta 1 , and fibronectin were significantly enhanced in the patients . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
RT PCR was done to assess renal expression of TGF beta 1 , collagen 1 and 3 , fibronectin , p 27 , p 21 and monocyte chemoattractant protein 1 ( MCP 1 ) . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Here , we investigate the effects of TGF beta 1 on laminin gamma 1 and fibronectin polypeptide expression and cell survival in mouse mesangial cells ( MES 13 ) . ^^^ TGF beta 1 ( 10 ng / ml ) stimulates laminin gamma 1 and fibronectin expression approximately two fold in a time dependent manner ( 0 48 h ) . ^^^ TGF beta 1 also elevates fibronectin glycosylation but its mobility is not altered . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
TGF beta 1 up regulates transglutaminase two and fibronectin in dermal fibroblasts : a possible mechanism for the stabilization of tissue inflammation . ^^^ In the denatured SDS PAGE , TGF beta 1 treatment resulted in marked induction of an approximately 220 kDa protein , which was revealed to be a fibronectin ( FN ) via western immunoblotting with an anti FN antibody . ^^^ Next , when the hDFs were treated with TGF beta 1 ( 1 ng / ml ) , FN expression was induced beginning at the third day after treatment . ^^^ Collectively , TGF beta 1 stimulates the polymerization of FN via the action of TGase 2 , which is supposed to to be an important mechanism in the stabilization of the inflammatory dermis . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
BAY 41 2272 administration resulted in marked reductions of glomerular and tubulointerstitial histological matrix accumulation , expression of TGF beta 1 and fibronectin , macrophage infiltration , and cell proliferation as well as improved renal function . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Both fibronectin and heparin , which are known to possess TGF beta 1 binding interactions , were found to increase VIC alpha SMA expression ( 120 % and 258 % of expression in controls ) , while VICs cultured on collagen 1 modified substrates had diminished alpha SMA expression ( 66 % of control ) . ^^^ The addition of heparin to fibronectin modified substrates led to a synergistic increase in VIC alpha SMA expression , produced by the reciprocal binding of fibronectin , heparin , cell produced TGF beta 1 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
We also examined effects of the chimeric ribozyme to TGF beta 1 on expression of TGF beta 1 , type 4 collagens , and fibronectin mRNAs and expression of TGF beta 1 protein in human gingival fibroblasts . ^^^ Chimeric ribozyme to TGF beta 1 significantly inhibited expression of TGF beta 1 , type 4 collagen , and fibronectin mRNAs and TGF beta 1 protein in human gingival fibroblasts . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Hachimi jio gan also reduced fibronectin and transforming growth factor beta 1 ( TGF beta 1 ) protein expression in the renal cortex . ^^^ These results suggested that Hachimi jio gan could prevent diabetic kidney damage by reducing renal oxidative injury and expression of fibronectin and TGF beta 1 proteins , which are all involved in the pathophysiology of diabetic nephropathy . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
After 6 weeks of administration of Benazepril or Losartan , the mRNA expressions of TGF beta 1 , Col 4 , Fn , ET 1 and iNOS in renal cortex were measured by RT PCR . ^^^ Besides , the expressions of TGF beta 1 , ET 1 and iNOS at protein level were detected by Western blotting and the concentrations of Col 4 and Fn were analyzed with immunohistochemistry respectively . ^^^ Renal cortex TGF beta 1 , Col 4 , Fn , ET 1 and iNOS in rats of group D were increased by 3 . 59 , 2 . 57 , 2 . 21 , 2 . 58 and 3 . 28 times at mRNA level , and by 2 . 60 , 1 . 40 , 0 . 75 , 1 . 83 and 2 . 15 times at protein level , respectively , compared with group C . ^^^ When the animals were treated with Benazepril ( group DB ) or Losartan ( group DL ) , however , the biochemical and pathological damages were significantly recovered , and protein expressions of TGF beta 1 , Col 4 , Fn , ET 1 and iNOS were also significantly diminished ( p < 0 . 05 ) . ^^^ This study suggested that blocking RAS using Benazepril or Losartan can have protective effects on the renal injury in glomerulosclerosis by down regulating the expressions of TGF beta 1 , Col 4 , Fn , ET 1 and iNOS . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
In human mesangial cells 9 cis RA suppressed TGF beta 1 induced alpha smooth muscle actin , fibronectin , and plasminogen activator inhibitor 1 expression , but it did not significantly affect cell proliferation and survival . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Exposure of GMCs to high glucose significantly stimulated the ECM protein , collagen and fibronectin , accumulation and TGF beta 1 secretion , and these changes were dramatically diminished by treatment of GMCs with extracts of Cnidii rhizoma or Tabanus ( 10 microg / ml ) . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
TGF beta 1 was also shown to stimulate the secretion of FN in cultured supernatant and the CTGF mRNA expression of NRK52E cells . ^^^ The addition of HGF inhibited the TGF beta 1 induced TEMT , the secretion of FN , and the CTGF expression of NRK52E cells , there was a significant correlation between the expression of CTGF and the expression of alpha SMA . ^^^ CONCLUSION : HGF could block TEMT and FN secretion triggered by TGF beta 1 , which implies that HGF could participate in renal interstitial fibrosis as a negative regulator . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
The conditioned supernatant was collected and subjected to ELISA assay for detection of fibronectin and TGF beta 1 . ^^^ In addition , albumin significantly upregulated the secretion of both fibronectin and TGF beta 1 at dose over 1 mg / ml . ^^^ Moreover , 24 h pretreatment of albumin significantly enhanced exogenous TGF beta 1 induced secretion of fibronectin . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
In contrast to high glucose conditions , increases in fibronectin synthesis that were stimulated by glycated albumin were not dependent on THBS 1 activation of latent TGFB 1 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
The expression of potential genes driving this process was quantified by Q RT PCR ( TGF beta 1 , fibronectin , its EDA splice variant , and PAI 1 ) . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Incubation of mesangial cells with natural or synthetic PPAR gamma agonists 15 deoxy Delta 12 , 14 prostaglandin J 2 ( 15d PGJ 2 ) or troglitazone and ciglitazone suppressed TGF beta 1 mediated alpha smooth muscle actin , fibronectin , and plasminogen activator inhibitor 1 expression . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Our results show that high glucose levels , TGF beta 1 , and ET 1 upregulated EDB ( + ) FN expression . ^^^ Treatment of cells exposed to high glucose with TGF beta 1 neutralizing antibody and ET receptor antagonist prevented high glucose induced EDB ( + ) FN expression . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
METHODS : We analysed the collagen content in terminal failing ventricles of VAD patients and donor hearts using 4 hydroxyproline for total collagen and real time RT PCR for fibronectin ( FN ) , collagen 1 alpha 1 ( Col1A1 ) , 3 alpha 1 ( Col3A1 ) and TGF beta 1 analysis . ^^^ RESULTS : Compared to donor hearts we found similar increases in Col1A1 and TGF beta 1 but not Col3A1 and FN mRNAs , which were similar in the myocardium from patients receiving a VAD or heart transplant . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
RESULTS : Naringenin reduced not only the accumulation of ECM , including collagen Ialpha 1 ( Col Ialpha 1 ) , fibronectin ( FN ) , and plasminogen activator inhibitor 1 ( PAI 1 ) , but also the production of Smad 3 induced by TGF beta 1 in both mRNA and protein levels in a dose dependent manner . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Human or rat mesangial cells ( MCs ) were treated with human recombinant renin ( HrRenin ) or rat recombinant renin ( RrRenin ) and the effects on TGF beta 1 , plasminogen activator inhibitor type 1 ( PAI 1 ) , fibronectin ( FN ) and collagen 1 mRNA and protein were investigated . ^^^ Renin induced increases in TGF beta 1 in turn stimulate increases in PAI 1 , FN and collagen 1 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Adhesion to fibronectin , laminin and type 1 collagen , and Matrigel invasion after stimulation with saline , IL 1beta and TGF beta 1 were evaluated using three primary and three metastatic pancreatic cancer cell lines . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
The expression levels of transforming growth factor beta 1 ( TGF beta 1 ) , connective tissue growth factor ( CTGF ) , fibronectin ( FN ) and plasminogen activator inhibitor 1 ( PAI 1 ) were detected by real time quantitative reverse transcriptase polymerase chain reaction ( RT PCR ) at the end of the experiment . ^^^ The expression levels of TGF beta 1 , CTGF , PAI 1 and FN in the untreated group were also significantly higher as compared with those in the normal control group ( P < 0 . 05 ) . ^^^ UAER , Ccr , VG , KW / BW ratio and the levels of TGF beta 1 , CTGF , PAI 1 and FN in the Salvia miltiorrhiza treated group were obviously lower than those in the untreated group ( P < 0 . 05 ) . ^^^ CONCLUSION : Salvia miltiorrhiza can protect rats with streptozotocin diabetes from diabetic nephropathy by suppressing the over expressions of TGF beta 1 , CTGF , PAI 1 and FN in renal cortex . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Immunohistochemical studies for TGF beta 1 , collagen type 1 , and fibronectin revealed that the combined treatment abolished glomerular fibrotic findings compared with candesartan . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
OBJECTIVES : To clarify the correlation of connective tissue growth factor ( CTGF ) expression and fibronectin ( FN ) synthesis after transforming growth factor beta 1 ( TGF beta 1 ) stimulation in human prostate stromal cells . ^^^ The gene and protein expressions of CTGF and FN after TGF beta 1 treatment were examined . ^^^ We also investigated the effect of CTGF blockade on TGF beta 1 induced FN synthesis by CTGF antisense oligodeoxynucleotide treatment . ^^^ The upregulation of both CTGF and FN protein by TGF beta 1 treatment was demonstrated in a dose dependent manner . ^^^ CTGF antisense oligodeoxynucleotide inhibited TGF beta 1 stimulated FN synthesis . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
RT PCR revealed that IL 10 gene therapy attenuated liver TGF beta 1 , collagen alpha 1 , fibronectin , and cell adhesion molecule mRNA upregulation . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Furthermore , long term administration of Hachimi jio gan reduced renal cortical expression of proteins , such as transforming growth factor beta 1 ( TGF beta 1 ) , fibronectin , inducible nitric oxide synthase and cyclooxygenase 2 . ^^^ The 100 and 200 mg kg ( 1 ) daily doses of Hachimi jio gan significantly reduced TGF beta 1 and fibronectin protein expression to levels below those of LETO rats . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
In vitro , treatment of human biliary epithelial cells with TGF beta 1 induced alphaSMA and fibronectin expression and suppressed cytokeratin 19 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
In addition , the ability of fibroblasts to release TGF beta 1 , fibronectin , and vascular endothelial growth factor ( VEGF ) was assessed by enzyme linked immunosorbent assay . ^^^ RNS also significantly increased TGF beta 1 ( P < 0 . 01 ) , fibronectin ( P < 0 . 01 ) , and VEGF ( P < 0 . 01 ) release into the media in both 3D gel and monolayer culture . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
The histologic improvements were accompanied by the suppression of gene and protein expression of fibronectin , interstitial collagen , and TGF beta 1 in the nephritic kidney . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Pretreatment of HEp 2 cells with TGF beta 1 increased their capacity to ingest GAS when the bacteria expressed fibronectin binding proteins ( M 1 or PrtF 1 ) . ^^^ TGF beta 1 regulates expression of integrins , Fn , and other extracellular matrix proteins , and positively controls the integrin signaling pathway . ^^^ Western blots revealed significant induction of alpha 5 integrin and Fn expression by HEp 2 cells in response to TGF beta 1 . ^^^ Increased ingestion of streptococci by these cells was blocked by a specific inhibitor of the TGF beta 1 receptor 1 and antibodies directed against alpha 5 integrin and Fn , indicating that increased invasion depends on TGF beta 1 up regulation of both the alpha 5 integrin and Fn . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Aortic TGF beta 1 type 2 receptor ( TbetaRII ) , its downstream molecules p similar to mad mother against decapentaplegic ( SMAD ) 2 / 3 and SMAD 4 , fibronectin , and collagen also increased with age . ^^^ Incubation of young aortic rings ex vivo or VSMCs in vitro with activated MMP 2 enhanced active TGF beta 1 , collagen , and fibronectin expression to the level of untreated old counterparts , and this effect was abolished via inhibitors of MMP 2 . ^^^ Interestingly , in old untreated rings or VSMCs , the increased TGF beta 1 , fibronectin , and collagen were also substantially reduced by inhibition of MMP 2 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
METHODS : The concentrations of EGF , TGF beta 1 , PDGF AB , fibronectin , vitamin A and vitamin E in serum , FFP , and platelet releasate were evaluated with ELISA and HPLC . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
METHODS : The effects of IL 7 on ECM production , immunolocalization of Smads , type 1 collagen , fibronectin , alpha smooth muscle actin ( alpha SMA ) , and cell migration were examined in human subconjunctival fibroblast culture with or without TGF beta 1 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
TGF beta 1 mediated endothelial to mesenchymal transformation correlated with enhanced expression of laminin and fibronectin within scaffolds evidenced by Western blotting . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Aldosterone promotes fibronectin production through a Smad 2 dependent TGF beta 1 pathway in mesangial cells . ^^^ Blocking TGF beta 1 signaling pathway by knocking down Smad 2 significantly blunted the aldosterone induced fibronectin production . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Aldose reductase regulates TGF beta 1 induced production of fibronectin and type 4 collagen in cultured rat mesangial cells . ^^^ RESULTS : The normal MsC showed increased expression of fibronectin and type 4 collagen with stimulation of TGF beta 1 . ^^^ CONCLUSION : AR can regulate the expression of fibronectin and type 4 collagen with the stimulation of TGF beta 1 in MsC , which may have relations with the activation of JNK MAPK and p 38 MAPK signalling pathways and AP 1 . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
LV levels of transforming growth factor beta 1 ( TGF beta 1 ) , procollagen 1 ( C 1 ) , and fibronectin ( FN ) mRNA were measured by Northern blotting . ^^^ LV expression of TGF beta 1 , C 1 , and FN mRNA was approximately 2 fold , approximately 2 . 5 fold , and approximately 5 fold greater , respectively , in hearts of AC 5 compared to SH 5 rats 3 days post operation , and was not significantly decreased by either dose of RSV . ^^^ Inhibition of HMG CoA reductase does not attenuate the pronounced aortic constriction induced increases in the early expression of TGF beta 1 , C 1 , and FN in this model of acute pressure overload of the rat heart . . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Grafts were harvested at 30 , 90 and 140 days for histological and gene expression analyses with respect to ED B FN , ET 1 and transforming growth factor beta 1 ( TGF beta 1 ) mRNA . ^^^ Treatment of allograft recipient rats with bosentan prevented upregulation of ED B FN and TGF beta 1 . ^^^ We further show that total FN , ED B FN , ET 1 and TGF beta 1 mRNA expression were upregulated in human chronic allograft nephropathy specimens . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Ex vivo , primary alveolar epithelial cells cultured on provisional matrix components , fibronectin or fibrin , undergo robust EMT via integrin dependent activation of endogenous latent TGF beta 1 . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
We show that incubation of the human bronchial epithelial cell line , BEAS 2B , or primary cultured human bronchial epithelial cells , normal human bronchial epithelial cells , with subcytotoxic concentrations ( 0 . 1 , 0 . 3 , and 1 microM ) of major basic protein ( MBP ) , or eosinophil peroxidase ( EPO ) , augmented the transcripts of endothelin 1 , TGF alpha , TGF beta 1 , platelet derived growth factor ( PDGF ) beta , epidermal growth factor receptor , metalloproteinase ( MMP ) 9 , fibronectin , and tenascin . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Our results demonstrated that the JNK pathway is required in the TGF beta 1 enhancement of uPA , fibronectin , E cadherin delocalization , actin re organization and vimentin expression , concomitant with the induction of cell migration . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
HCC and HPBL have clearly different patterns of gene expression , with genes IGF 2 , Fibronectin , DLK 1 , TGFb 1 , MALAT 1 and MIG 6 being over expressed in HPBL versus HCC . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
Superoxide , not nitric oxide or hydrogen peroxide , mediated high glucose and AGE induced TGF beta 1 and fibronectin expression . ^^^ Exogenous SOD treatment in diabetic rats significantly attenuated diabetes induction of superoxide , urinary albumin excretion , 8 hydroxy 2 ' deoxyguanosine , TGF beta 1 , and fibronectin immunoreactivities in renal glomerular mesangial cells . ^^^
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
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Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15582 and P02751	Pubmed	SVM Score :0.0
NA