| Interacting proteins: Q14766 and P02751 |
Pubmed |
SVM Score :1.1325118 |
| The association of LTBP 1 with cellular fibronectin was abolished by treatment of the medium with plasmin , which cleaves LTBP 1 and inhibits its assembly to matrix . 1.1325118^^^ |
|
| Interacting proteins: Q14766 and P02751 |
Pubmed |
SVM Score :0.0 |
| Fibrillin containing microfibrils separated at 1 . 37 g / ml and copurified with MAGP 1 , but not LTBP 1 , LTBP 2 , or fibronectin . ^^^ |
|
| Interacting proteins: Q14766 and P02751 |
Pubmed |
SVM Score :0.0 |
| Both anti LTBP 1 antibody ( Ab 39 ) and synthetic oligopeptides inhibited the stretch induced mRNA expression of type 1 collagen and fibronectin in a dose dependent manner , but the inhibition by Ab 39 or the oligopeptides was recovered by adding recombinant TGF beta . ^^^ |
|
| Interacting proteins: Q14766 and P02751 |
Pubmed |
SVM Score :0.0 |
| Early colocalization of LTBP 1 with fibronectin suggested a role for fibronectin in the initial assembly of LTBP 1 into the matrix ; however , in more differentiated osteoblast cultures , LTBP 1 and fibronectin 1 were found in distinct fibrillar networks . ^^^ |
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| Interacting proteins: Q14766 and P02751 |
Pubmed |
SVM Score :0.0 |
| LTBP 1 mRNA and protein were overexpressed in the fibrotic region and colocalized with other indicators of fibrosis such as collagen and fibronectin and the fibroblast marker proteins alpha actin and vimentin . ^^^ |
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| Interacting proteins: Q14766 and P02751 |
Pubmed |
SVM Score :0.0 |
| Here we show that fibronectin is critical for the incorporation of LTBP 1 and transforming growth factor beta ( TGF beta ) into the ECM of osteoblasts and fibroblasts . ^^^ Immunolocalization studies suggested that fibronectin provides an initial scaffold that precedes and patterns LTBP 1 deposition but that LTBP 1 and fibronectin are later localized in separate fibrillar networks , suggesting that the initial template is lost . ^^^ Treatment of fetal rat calvarial osteoblasts with a 70 kDa N terminal fibronectin fragment that inhibits fibronectin assembly impaired incorporation of LTBP 1 and TGFbeta into the ECM . ^^^ Consistent with this , LTBP 1 failed to assemble in embryonic fibroblasts that lack the gene for fibronectin . ^^^ LTBP 1 assembly was rescued by full length fibronectin and superfibronectin , which are capable of assembly into fibronectin fibrils , but not by other fibronectin fragments , including a 160 kDa RGD containing fragment that activates alpha5beta1 integrins . ^^^ |
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| Interacting proteins: Q14766 and P02751 |
Pubmed |
SVM Score :0.0 |
| Fibronectin is required for integrin alphavbeta 6 mediated activation of latent TGF beta complexes containing LTBP 1 . ^^^ The LTBP 1 polypeptide rL1N , which includes the hinge , associates with fibronectin in binding assays . ^^^ |
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| Interacting proteins: Q14766 and P02751 |
Pubmed |
SVM Score :0.0 |
| Increased tTgase expression led to an increased rate of LTBP 1 deposition in the matrix , which was accompanied by an increased pool of deoxycholate insoluble fibronectin . ^^^ LTPB 1 was also found to co localize with both intracellular and extracellular fibronectin , and increased immunoreactivity for LTBP 1 and fibronectin was found in large molecular weight polymers in the deoxycholate insoluble matrix of fibroblasts overexpressing tTgase . ^^^ We conclude that regulation of tTgase expression is important for controlling matrix storage of latent TGF beta 1 complexes and that fibronectin may be one extracellular component to which LTBP 1 is crosslinked when LTBP 1 and tTgase interact at the cell surface . ( J Histochem Cytochem 47 : 1417 1432 , 1999 ) . ^^^ |
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| Interacting proteins: Q14766 and P02751 |
Pubmed |
SVM Score :0.0 |
| LTBP 1 initially co distributes with fibronectin in the extracellular matrix of osteoblasts , and depends on fibronectin for its assembly . ^^^ To gain further insights into the mechanisms of assembly of LTBP 1 and its spatial and temporal interactions with fibronectin , we have performed dual fluorescence time lapse imaging of these two proteins in living osteoblasts using fluorescent probes . ^^^ Time lapse movies showed surprisingly large fibril displacements associated with cellular movement as well as occasional breaking of LTBP 1 or fibronectin containing fibrils . ^^^ |
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| Interacting proteins: Q14766 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
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