| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.83799539 |
| Extending our previous observation that tissue transglutaminase ( TGase ) binds to extracellular matrix ( ECM ) fibronectin , we report here that endogenous tissue TGase is localized on the adjacent ECM after puncture wounding embryonic human lung fibroblasts ( WI 38 ) . 0.83799539^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.84457099 |
| Tissue transglutaminase ( tTG ) has recently been established as a novel cell surface adhesion protein that binds with high affinity to fibronectin in the pericellular matrix . 0.84457099^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.75021344 |
| Specific association of tissue transglutaminase ( tTG ) with matrix fibronectin ( FN ) results in the formation of an extracellular complex ( tTG FN ) with distinct adhesive and pro survival characteristics . tTG FN supports RGD independent cell adhesion of different cell types and the formation of distinctive RhoA dependent focal adhesions following inhibition of integrin function by competitive RGD peptides and function blocking anti integrin antibodies alpha5beta1 . 0.75021344^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.50303541 |
| Using synthetic peptides encompassing this region , we found that the peptide 88WTATVVDQQDCTLSLQLTT106 inhibited the interaction of tissue transglutaminase with fibronectin and decreased transglutaminase dependent cell adhesion and spreading . 0.50303541^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| The dansyl conjugated ( Dns ) peptides Dns Pro Gly Gly Gln Gln Ile Val and Dns Ala Gln Gln Ile Val , patterned on the N terminal sequence of fibronectin , were synthesized and used for the transglutaminase ( protein glutamine : amine gamma glutamyltransferase , EC 2 . 3 . 2 . 13 ) directed selective blocking of lens proteins that otherwise might participate in donating lysyl side chains in forming N epsilon ( gamma glutamyl ) lysine cross linked oligomers and polymers . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| Peptides patterned on the N terminal sequence of fibronectin were synthesized and tested for amine acceptor qualities in reactions with dansylcadaverine catalyzed either by coagulation factor XIIIa or intracellular transglutaminase ( protein glutamine : amine gamma glutamyltransferase , EC 2 . 3 . 2 . 13 ) . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| Two plasma proteins , alpha 2 plasmin inhibitor and plasma fibronectin , are cross linked to fibrin by plasma transglutaminase ( R glutaminyl peptide : amine gamma glutamyl yltransferase , EC 2 . 3 . 2 . 13 , fibrin stabilizing factor ) when blood coagulation takes place . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| Guinea pig liver transglutaminase ( EC 2 . 3 . 2 . 13 ) displays a Ca ( 2+ ) independent binding ( Ka = 10 ( 7 ) M 1 ) to the same gelatin binding domain of human plasma fibronectin that is known to form a very tight complex with the human red cell enzyme . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| Complex formation between the human erythrocyte transglutaminase ( protein glutamine : amine gamma glutamyltransferase , EC 2 . 3 . 2 . 13 ) and fibronectin or its fragments was examined by immunoanalytical procedures and by fluorescence polarization . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| Complexation of fibronectin with tissue transglutaminase . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| Kinetic studies revealed that inhibitors of DPP 4 which enhanced fibronectin binding in vitro accelerated the initial binding of fibronectin to the cell surface where it was subsequently cross linked ( presumably by tissue transglutaminase ) to as yet undefined components . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| The sites of transglutamination of fibronectin and fibronectin fragments , by coagulation factor XIIIa and tissue transglutaminase , were studied . ^^^ It was shown that the intact fibronectin molecule has two sites sensitive to coagulation factor XIIIa and four sites sensitive to tissue transglutaminase : 180 190 kDa gelatin / heparin binding fragments , 2 and 5 6 sites ; 29 kDa heparin I / fibrin 1 binding N terminal fragments , 1 and 2 sites ; 70 kDa gelatin binding fragments , 0 and 1 site ; 60 kDa cell binding central fragments , 1 and 3 4 sites ; 60 kDa , 45 kDa , 30 kDa heparin 2 binding C terminal fragments , 1 and 2 sites . ^^^ Tissue transglutaminase appeared to interact with all of the three coagulation factor XIIIa sensitive sites and , in addition , some others which are either available on the intact molecule or can be revealed only in proteolytic fragments of the fibronectin . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| The results suggest a specific carrier function for fibronectin that might be of physiological importance in determining the fate of a tissue transglutaminase accidentally discharged into plasma . . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| The induction of fibronectin and tissue transglutaminase suggests that the TGF beta 1 pathway of cell death differs from that of normal ectocervical epithelial cell differentiation , which is mediated by epidermal transglutaminase . . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| The cross linking of the substrate immobilized [ 125I ] fibronectin was mediated by cell associated tissue transglutaminase and occurred more rapidly during the first 30 min after endothelial cell seeding but also continued for several hours after the cells were fully spread . ^^^ Thus , endothelial cells process fibronectin into cross linked multimers due to the expression of tissue transglutaminase at the basal surface of the cell . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| Met B clones expressing the exogenous tissue transglutaminase exhibited a reduced incidence of primary tumour formation and an increased adherence to tissue culture plastic and fibronectin coated surfaces when compared to transfected and non transfected control cells . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| Trophoblast transglutaminase was identified as a tissue transglutaminase by non denaturing gel electrophoresis and dansylcadaverine activity staining , fibronectin binding and Western blotting with a specific antibody . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| Peptides 4 and 7 also inhibited guinea pig liver tissue transglutaminase from cross linking fibrinogen , N , N ' dimethylcasein , and fibronectin . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| Reduced expression of tissue transglutaminase in a human endothelial cell line leads to changes in cell spreading , cell adhesion and reduced polymerisation of fibronectin . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| Regulated expression of tissue transglutaminase in Swiss 3T3 fibroblasts : effects on the processing of fibronectin , cell attachment , and cell death . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| On different tissue sections , binding of gliadin to reticular matrix components was observed which was Ca2+ dependent , inhibited by Cu2+ and Zn2+ ions , by putrescine , and by preincubation with antibodies against tissue transglutaminase ( tTG ) suggesting that binding of gliadin is mediated by tTG . tTG was able to bind to gliadin and fibronectin fixed to microplates . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| Cell surface localization of tissue transglutaminase is dependent on a fibronectin binding site in its N terminal beta sandwich domain . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| Tissue transglutaminase is an integrin binding adhesion coreceptor for fibronectin . ^^^ The protein cross linking enzyme tissue transglutaminase binds in vitro with high affinity to fibronectin via its 42 kD gelatin binding domain . ^^^ Overexpression of tissue transglutaminase increases its amount on the cell surface , enhances adhesion and spreading on fibronectin and its 42 kD fragment , enlarges focal adhesions , and amplifies adhesion dependent phosphorylation of focal adhesion kinase . ^^^ Together our results demonstrate that tissue transglutaminase mediates the interaction of integrins with fibronectin , thereby acting as an integrin associated coreceptor to promote cell adhesion and spreading . . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| PURPOSE : To study whether human trabecular meshwork ( HTM ) cells are capable of expressing and secreting tissue transglutaminase ( tTgase ) , an enzyme cross linking extracellular matrix ( ECM ) proteins , and whether tTgase and synthesis of cross linked fibronectin are increased after treatment of HTM cells with transforming growth factor ( TGF ) beta 1 or beta 2 . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| Tissue transglutaminase ( tTG ) is a sulfhydryl rich protein that is expressed by endothelial cells and secreted into the extracellular matrix ( ECM ) where it is bound to fibronectin . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| Cell surface tissue transglutaminase is involved in adhesion and migration of monocytic cells on fibronectin . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| We have previously shown that cell surface tissue transglutaminase serves as an integrin binding adhesion coreceptor for fibronectin . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| Localization of tissue transglutaminase ( tTgase ) , its reaction product epsilon ( gamma glutamyl ) lysine , and fibronectin was investigated immunohistochemically . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| Tissue transglutaminase ( tTG ) serves as a potent and ubiquitous integrin associated adhesion co receptor for fibronectin on the cell surface and affects several key integrin functions . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| Incorporation of fluorescein cadaverine into matrix proteins was accompanied by the cross linking of fibronectin and tissue transglutaminase into nonreducible high molecular weight polymers . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| We show that this is accompanied by a partial blockage of the transcriptional induction of proline rich tyrosine kinase 2 , a gene coding for a focal adhesion kinase active in monocytes , and of tissue transglutaminase , a gene coding for a fibronectin coreceptor in monocytes . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| TGF beta 2 induced cell surface tissue transglutaminase increases adhesion and migration of RPE cells on fibronectin through the gelatin binding domain . ^^^ In the present study , the functional role of cell surface tissue transglutaminase ( tTG ) in adhesion and migration of RPE cells on fibronectin ( Fn ) and collagen type 1 ( Col 1 ) after stimulation with TGF beta 2 was investigated . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| PURPOSE : To study whether glaucomatous extracellular matrix ( ECM ) modifications in the lamina cribrosa might be induced by TGF beta 2 , the effect of TGF beta 2 on the expression of collagen types 1 ( Col 1 alpha 1 ) , 3 ( Col 3 alpha 1 ) , and 4 ( Col 4 alpha 2 ) ; fibronectin ( FN ) ; tissue transglutaminase ( TGM 2 ) ; connective tissue growth factor ( CTGF ) ; and thrombospondin ( TSP 1 ) in cultured human optic nerve head ( ONH ) astrocytes was investigated . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| CONTEXT : Tissue transglutaminase ( tTG ) has a high affinity for fibronectin ( FN ) and is a coreceptor of both beta 1 and beta 3 integrin subunits . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| Tissue transglutaminase ( tTG ) is a multifunctional protein that serves as cross linking enzyme and integrin binding adhesion coreceptor for fibronectin on the cell surface . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| Enhanced cross linking of ECM by tissue transglutaminase and decreased degradation due to reduced active MMP 9 expression may be at least partially responsible for the deposition of FN within injured glomeruli in experimental FSGS . . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| On the other hand , the cell adhesion to TGc is mediated by alpha4beta1 integrin but not alpha5beta1 ; a CS 1 peptide , which represents the binding site of fibronectin to alpha4beta1 integrin , completely inhibited the cell adhesion to TGc . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| Here the authors sought to examine whether transglutaminase 2 ( TGase 2 ) is expressed in lens epithelial cells from patients with anterior polar cataracts , to determine whether TGase 2 expression is induced by transforming growth factor ( TGF beta ) in cultured lens epithelial cells , and to determine whether TGase 2 participates in the crosslinking of fibronectin in lens epithelial cells in vitro . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| METHODS : TG 2 knockout ( TG 2 / ) and wild type mouse oesophagus , jejunum , liver , and kidney sections , and TG 2 / sections coated with human recombinant TG 2 were used as substrates in single and double immunofluorescent studies for patient IgA binding and tissue localisation of TG 2 , fibronectin , actin , and calreticulin . ^^^ These positivities partly colocalised with actin and calreticulin but not with TG 2 or fibronectin . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| TG 2 activities , regulated by nucleotides and calcium , include cross linking of cartilage matrix proteins , binding of fibronectin , and hydrolysis of GTP and ATP . ^^^ Following transfection of TG 2 site directed mutants into chondrocytic cells , we observed that wild type TG 2 , and TG catalytic site and fibronectin binding mutants promoted type 10 collagen expression and matrix calcification consistent with chondrocyte hypertrophic differentiation . ^^^ In conclusion , externalized GTP bound TG 2 serves as a molecular switch for differentiation of chondrocytes to a hypertrophic , calcifying phenotype in a manner that does not require either TG 2 transamidation activity or fibronectin binding . . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| Another important property of TG 2 is that it has high binding affinity for the ECM component protein , fibronectin and thus can promote interaction between cell surface integrin with fibronectin . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| The incubation of TG 2 expressing drug resistant MCF 7 cells on fibronectin ( Fn ) coated surfaces strongly activated focal adhesion kinase , an event that leads to the activation of several downstream signaling pathways and , in turn , can confer apoptosis resistant phenotype to cancer cells . ^^^ The role of TG 2 in Fn mediated cell attachment , cell growth , and cell survival functions was further analysed by small interfering RNA ( siRNA ) approach . ^^^ Inhibition of TG 2 by siRNA inhibited Fn mediated cell attachment and cell survival functions in drug resistant MCF 7 cells . ^^^ We conclude that the expression of TG 2 in breast cancer cells contributes to the development of the drug resistance phenotype by promoting interaction between integrins and Fn . . ^^^ |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21980 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|