| Interacting proteins: P17936 and P02751 |
Pubmed |
SVM Score :0.83882522 |
| Using purified proteins we demonstrated that IGFBP 3 binds to fibronectin in a dose dependent manner , but not to tenascin C . 0.83882522^^^ Using immunofluorescent staining and confocal microscopy we found that IGFBP 3 accumulates with increasing age in the chondrocyte territorial matrix where it co localizes with fibronectin , but not with tenascin C or type 6 collagen . 0.54238744^^^ |
|
| Interacting proteins: P17936 and P02751 |
Pubmed |
SVM Score :0.60947747 |
| We found that IGFBP 3 accumulated around chondrocytes in the pericellular / territorial matrix , where it co localized with fibronectin , but not with the other matrix proteins tenascin C and type 6 collagen . 0.60947747^^^ |
|
| Interacting proteins: P17936 and P02751 |
Pubmed |
SVM Score :0.87814723 |
| In agarose diffusion assays IGFBP 3 interacted with fibronectin and heparan sulfate proteoglycan but not with type 6 collagen or tenascin C . 0.87814723^^^ |
|
| Interacting proteins: P17936 and P02751 |
Pubmed |
SVM Score :0.0 |
| Mouse testicular Leydig cells express Klk 21 , a tissue kallikrein that cleaves fibronectin and IGF binding protein 3 . ^^^ The enzyme activity was strongly inhibited with typical serine protease inhibitors . mKlk 21 hydrolyzed casein , gelatin , fibronectin , and IGF binding protein 3 ( IGFBP 3 ) . ^^^ |
|
| Interacting proteins: P17936 and P02751 |
Pubmed |
SVM Score :0.0 |
| Upon measuring the expression of two progesterone regulated genes ( fibronectin and IGF binding protein 3 ) , tibolone , the Delta ( 4 ) isomer and medroxyprogesterone acetate showed similar gene expression regulation . ^^^ |
|
| Interacting proteins: P17936 and P02751 |
Pubmed |
SVM Score :0.0 |
| The known genes included alpha 1 ( 1 ) procollagen , alpha 2 ( 1 ) procollagen , fibronectin , ferritin heavy chain , insulinlike growth factor binding protein 3 ( IGFBP 3 ) , osteonectin , human tissue plasminogen activator inhibitor type 1 , thrombospondin , and alpha B crystallin . ^^^ |
|
| Interacting proteins: P17936 and P02751 |
Pubmed |
SVM Score :0.0 |
| In this study , we have demonstrated that IGFBP 3 binding on the cell surface is specific and receptor mediated , by showing : 1 ) a dose dependent increase of IGFBP 3 binding by addition of divalent cations ( CaCl 2 and MnCl 2 ) ; 2 ) dose dependent competition of 125I IGFBP 3E . coli by unlabeled IGFBP 3E . coli ( > 80 % competition at 100 nM ) , but not by IGFBP 1 or fibronectin . ^^^ |
|
| Interacting proteins: P17936 and P02751 |
Pubmed |
SVM Score :0.0 |
| Concentrations of IGF 1 and alpha 2 M in human seminal plasma were related to semen quality , basal concentrations of HGH , testosterone , IGFBP 3 , soluble fibronectin receptor ( sFNR ) , PSA and PP 14 in seminal plasma and to serum concentrations of luteinizing hormone ( LH ) and follicle stimulating hormone ( FSH ) . ^^^ |
|
| Interacting proteins: P17936 and P02751 |
Pubmed |
SVM Score :0.0 |
| Similarly , IGFBP 3 decreased from 4 . 0+ / 0 . 2 mg l ( 1 ) before chemotherapy to 3 . 0+ / 0 . 3 mg l ( 1 ) during FN ( p = 0 . 01 ) , and returned to 4 . 1+ / 0 . 2 mg l ( 1 ) at 6 months ( p = 0 . 01 ) . ^^^ Scanning densitometry showed a decrease in IGFBP 3 from 94 to 54 % during FN , when the presence of IGFBP 3 protease activity was observed . ^^^ |
|
| Interacting proteins: P17936 and P02751 |
Pubmed |
SVM Score :0.0 |
| Intrinsic actions of IGFBP 3 and IGFBP 5 on Hs578T breast cancer epithelial cells : inhibition or accentuation of attachment and survival is dependent upon the presence of fibronectin . ^^^ Attachment to fibronectin , however , was increased by IGFBP 3 and decreased by IGFBP 5 . ^^^ The actions of IGFBP 3 and 5 on cell attachment to ECM were lost in the presence of a soluble Arg Gly Asp ( RGD ) containing fibronectin fragment . ^^^ With cells grown on fibronectin , the action of IGFBP 3 was reversed , and it conferred cell survival , whereas the survival effect of IGFBP 5 was lost . ^^^ These effects on cell survival are paralleled by acute effects on integrin receptor function ; IGFBP 3 and 5 were able to either enhance or inhibit cell attachment in the presence of fibronectin . ^^^ |
|
| Interacting proteins: P17936 and P02751 |
Pubmed |
SVM Score :0.0 |
| Differentially regulated genes include those involved in cell matrix interactions including proline 4 hydroxylase ( P4HA2 ) , galectin 1 ( LGALS 1 ) and galectin 3 ( LGALS 3 ) , fibronectin 1 ( FN 1 ) and p cadherin ( CDH 3 ) , and cell proliferation ( CRIP 1 , IGFBP 3 ) and transformation ( S100P , S100A4 ) . ^^^ |
|
| Interacting proteins: P17936 and P02751 |
Pubmed |
SVM Score :0.0 |
| The same was true between BMD and IGFBP 3 in 2 skeletal sites ( LS , FN ) . ^^^ |
|
| Interacting proteins: P17936 and P02751 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Sixty six transcripts were overexpressed > or =3 fold in PENs compared with normal islet cells , including putative oncogenes ( MLLT10 / AF10 ) , growth factors [ insulin like growth factor binding protein 3 ( IGFBP 3 ) ] , cell adhesion and migration molecules ( fibronectin ) , and endothelial elements ( MUC18 / MelCAM and CD 31 ) . ^^^ Using PEN tissue microarrays , we confirmed the differential up regulation of IGFBP 3 ( 70 % ) and fibronectin ( 22 % ) and differential down regulation of p 21 ( 46 % ) and MIC 2 ( CD 99 ; 91 % ) in PENs versus normal pancreatic islets . ^^^ |
|
| Interacting proteins: P17936 and P02751 |
Pubmed |
SVM Score :0.0 |
| Consistent with this we have demonstrated that although IGFBP 3 and a non IGF binding fragment ( serine phosphorylation domain peptide ) reduced attachment and enhanced apoptosis of Hs578T breast cancer cells cultured on collagen or laminin , it promoted their attachment and survival on fibronectin , which is abundant in the matrix of aggressive tumors . ^^^ In contrast , IGFBP 3 promoted growth and survival for nonmalignant MCF 10A cells , but these effects were again reversed on fibronectin and blocked by the beta 1 antibody or a MAPK inhibitor or by disruption of cholesterol stabilized complexes . ^^^ |
|
| Interacting proteins: P17936 and P02751 |
Pubmed |
SVM Score :0.0 |
| Previous studies have shown that in solution , IGFBP 3 binds constituent proteins of the extracellular matrix ( ECM ) such as fibronectin and collagen and is present in ECM deposited by fibroblasts in vitro ; however , binding of IGFBP 3 to matrix has not been characterized , nor has its function in this environment been investigated . ^^^ |
|
| Interacting proteins: P17936 and P02751 |
Pubmed |
SVM Score :0.0 |
| PSA is a serine protease with substrates that include semenogelin 1 and 2 , insulin like growth factor binding protein 3 , fibronectin , and laminin . ^^^ |
|
| Interacting proteins: P17936 and P02751 |
Pubmed |
SVM Score :0.0 |
| Sequencing of the partially enriched cDNAs identified 36 genes differentially expressed , including ECM proteins ( pro alpha ( 1 ) collagen type 3 , fibronectin ) , structural protein ( spectrin ) , enzyme ( GTP specific succinyl CoA synthetase ) , transcriptional regulators ( glucocorticoid induced leucine zipper , inhibitor of DNA binding 3 ) and proteins involved in cell division control ( cdc 2 ) or cell signalling ( insulin like growth factor binding protein 3 , mutant p 53 binding protein , grp 75 , CGI 121 , protein phosphatase type 2A , tspan 2 and Sam 68 ) . 4 . ^^^ |
|
| Interacting proteins: P17936 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P17936 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P17936 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P17936 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P17936 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P17936 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P17936 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P17936 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P17936 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P17936 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P17936 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P17936 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P17936 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|