Pubmed abstracts for Protein-Protein Interaction search result :


Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Human myeloma cells adhere to fibronectin in response to hepatocyte growth factor . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Adhesion of myeloma cells to the bone marrow stroma matrix protein fibronectin is stimulated by hepatocyte growth factor . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
The purified metalloproteases of Serratia marcescens strains SF 178 and SH 186 attacked soluble human serum fibronectin . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
The minimum SF ( MSF ) value attained was not correlated with the appearance of iron deficiency . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Fibronectin protects both HSF and HGF from the effects of extracellular ATP . . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
HGF also inhibited TGF beta 1 mediated fibronectin and type 1 collagen expression . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Furthermore , HGF induces PI3K dependent alpha4beta1 integrin mediated adhesion to VCAM 1 and fibronectin . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.90438659
METHODS : 125I labeled HGF was incubated with collagens 1 6 , single collagen chains and their cyanogen bromide peptides , with fibronectin , fibrinogen , and laminin that were either immobilized on polystyrene or blotted to nitrocellulose after sodium dodecyl sulfate polyacrylamide gel electrophoresis . 0.90438659^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
We investigated the effects of human placental scatter factor ( hSF ) , mouse scatter factor ( mSF ) and recombinant human hepatocyte growth factor ( HGF ) on motility and morphology of individual Madin Darby canine kidney cells using a computerized cell tracking system . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Constitutive expression is repressed at 1 hour for transcripts that encode receptors for fibronectin ( FN ) , epidermal growth factor , and hepatocyte growth factor ( c met ) , and the secreted proteins FN and osteopontin . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
By Northern blot analysis of total RNA , extracted from normal human and rat Ito cells , either freshly isolated or in culture , recent studies demonstrated the expression of mRNA for collagen type 1 , 3 , 4 , fibronectin , laminin , transforming growth factor beta ( TGF beta ) , hepatocyte growth factor , nuclear retinoic acid receptor beta and alpha smooth m muscle actin . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
FCM activity did not appear to be accounted for by hepatocyte growth factor , keratinocyte growth factor , acidic fibroblast growth factor , or fibronectin . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
An anti alphavbeta 6 monoclonal antibody 10D5 , generated by immunization of beta 6 / mice with murine keratinocytes , inhibited adhesion and migration of wild type keratinocyte on both vitronectin and fibronectin to levels similar to those seen with keratinocytes from beta 6 / mice . alphavbeta 6 mediated migration on both ligands was dramatically augmented by treatment with phorbol myrisate acetate ( PMA ) or with hepatocyte growth factor , and augmentation of migration by either stimulus could be abolished by the PKC inhibitor GF109203X , suggesting a critical role for PKC in enhancement of alphavbeta 6 mediated cell migration . . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Hepatocyte growth factor stimulates migration of type 2 alveolar epithelial cells on the provisional matrix proteins fibronectin and fibrinogen . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Cooperative effect of hepatocyte growth factor and fibronectin in anchorage independent survival of mammary carcinoma cells : requirement for phosphatidylinositol 3 kinase activity . ^^^ We showed previously that the addition of exogenous hepatocyte growth factor ( HGF ) and the presence of fibronectin fibrils stimulate anchorage independent colony growth of a murine mammary carcinoma , SP 1 , which expresses both HGF and HGF receptor ( Met ; R . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
In addition to adsorption of LDL , DSC adsorbs plasma constituents that have the following characteristics : proteins containing apolipoprotein B ( Lp [ a ] ) ; proteins involved in the initial contact phase of the intrinsic coagulation pathway ( coagulation factor 12 , high molecular weight kininogen and prekallikrein ) ; factors with lipophilic characteristics ( coagulation factor 7 , coagulation factor 8 , and vitamin E ) ; and proteins with adhesive or other characteristics ( von Willebrand factor , fibronectin , serum amyloid P component , hepatocyte growth factor ) . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
However , whereas fibronectin , bFGF , hepatocyte growth factor , and TGF beta 1 significantly augmented migration of mouse myoblasts , TNF alpha did not , nor did PDGF BB or IGF 1 . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
RESULTS : Hepatocyte growth factor stimulated hyaluronic acid production , reduced collagen type 1 production , and did not affect fibronectin production , while transforming growth factor beta 1 stimulated the productions of all components . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Hepatocyte growth factor receptor , matrix metalloproteinase 11 , tissue inhibitor of metalloproteinase 1 , and fibronectin are up regulated in papillary thyroid carcinoma : a cDNA and tissue microarray study . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
In vivo and in vitro studies of the murine mammary gland have provided insights into novel stroma dependent mechanisms by which estrogen and progesterone action in the epithelium can be modulated by hepatocyte growth factor ( HGF ) and the extracellular matrix proteins , collagen type 1 , fibronectin and laminin . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
RESULTS : PLT releasate contains more epithelial growth factor , PLT derived growth factor , and transforming growth factor beta , but less hepatocyte growth factor , fibronectin , and vitamins . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Effects of hepatocyte growth factor on IL 1alpha triggered tubular epithelial myofibroblast transdifferentiation and fibronectin secretion in vitro ] . ^^^ OBJECTIVE : To observe the effects of hepatocyte growth factor ( HGF ) on tubular epithelial myofibroblast transdifferentiation ( TEMT ) triggered by IL 1alpha and the fibronectin secretion of TEMT . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Novel hepatocyte growth factor ( HGF ) binding domains on fibronectin and vitronectin coordinate a distinct and amplified Met integrin induced signalling pathway in endothelial cells . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Fibronectin hepatocyte growth factor enhances reendothelialization in tissue engineered heart valve . ^^^ We developed a new decellularized valve containing a fusion protein combined fibronectin and hepatocyte growth factor . ^^^ Fibronectin hepatocyte growth factor was introduced into the decellularized valves . ^^^ The decellularized valves with fibronectin hepatocyte growth factor were implanted into the pulmonary arterial trunk of dogs ( F group : n = 15 ) . ^^^ CONCLUSIONS : This study demonstrated that fibronectin hepatocyte growth factor enhanced early in situ recellularization in decellularized valves . . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Examples include : 1 ) scatter factor ( SF ) , a mesenchymal cell derived protein which causes contiguous sheets of epithelium to separate into individual cells and stimulates the migration of epithelial as well as vascular endothelial cells ; 2 ) autocrine motility factor ( AMF ) , a tumor cell derived protein which stimulates migration of the producer cells ; and 3 ) migration stimulating factor ( MSF ) , a protein produced by fetal and cancer patient fibroblasts which stimulates penetration of three dimensional collagen gels by non producing adult fibroblasts . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
We have recently shown that human SF antigen is identical to what has been known as the `` cold insoluble globulin ' ' and that it shows affinity toward fibrin and fibrinogen . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
It is concluded from these studies that the large external transformation sensitive ( LETS ) protein detected by other workers is the same molecule as SF 210 . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Basal fibronectin production ( which increased with the duration of incubation ) was significantly greater ( p < 0 . 001 ) in granulosa cells derived from mature follicle ( F 1 ) than in F 3 or SF cells . ^^^ Both LH and forskolin stimulated fibronectin production in SF and F 3 cells in a dose dependent manner ; however , they were without effect in F 1 cells . ^^^ The magnitude of increase in fibronectin production elicited by LH or forskolin was greater in SF cells than in F 3 cells . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
The role of VLA 4 fibronectin ( FN ) receptors in the interaction of activated SF T cells from RA patients with a 38 kD fragment of FN has been previously demonstrated . ^^^ A significant higher proportion of SF T cells were able to bind to an 80 kD fragment of FN , containing the Arg Gly Asp ( RGD ) cell binding site , compared with PB T cells . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
To investigate the role of Tc FN interactions in synovitis the binding of synovial fluid ( SF ) and peripheral blood ( PB ) T cells to FN coated wells , and the expression of cell surface VLA molecules on these cells by double label immunofluorescence , were studied . ^^^ SF T cells bound better to FN than PB T cells . ^^^ Anti VLA alpha 4 , VLA beta 1 and VLA alpha 5 MoAbs inhibited the binding of SF T cells to FN . ^^^ The increased binding of SF T cells to FN could have been related to activation and / or to their predominantly memory phenotype . ^^^ In contrast , compared with SF T cells , concanavalin A activated T cells showed a very similar level of binding to FN , comparable expression of VLA molecules and the same pattern of inhibition of binding to FN by MoAbs . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Since fibronectin levels in the synovial fluid ( SF ) and on the cartilage surface of patients with osteoarthritis ( OA ) have been shown to be greatly increased over normal levels and since protease levels are also enhanced in diseased cartilage , we have investigated the presence of fibronectin fragments in the SF of patients with OA . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Furthermore , the majority of these activated SF T cells were able to adhere to a 38 kD FN proteolytic fragment containing the connecting segment 1 ( CS 1 ) specifically through VLA 4 receptors , whereas a significantly lower proportion of PB T cells displayed this capacity . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Migration of endothelial cells from beads , while extremely sensitive to SF , was not affected by serum concentration ( 1 to 10 % ) , various purified growth factors , or fibronectin . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
We propose that fibronectin collagen interactions are , in part , responsible for inducing endothelial synthesis of cytoskeletal proteins required for SF assembly . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Although fibronectin also was synthesized and secreted into the culture medium , HepG 2 cell spreading was observed in response to human serum SF , but not in response to human plasma fibronectin . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
In one approach , skin explants were placed on the bottom of plastic dishes coated with human FN , human fibrinogen ( FGN ) , human serum spreading factor ( SF ) , or bovine type 1 collagen . ^^^ In these experiments exposure to 50 micrograms ml 1 of RGDS reduced migration over FN , FGN and SF to 2 7 % of control levels . ^^^ These results indicate that newt epidermal cells possess RGDS receptors and that these receptors are involved in epidermal wound closure in vivo and in migration from skin explants onto plastic coated with FN , FGN , SF and collagen . ^^^ The relative RGDS insensitivity of wound closure in vivo and in migration from explants onto collagen may reflect in these instances the presence of a relatively high density of RGDS receptor binding sites on the substrate ; the presence of RGDS receptor binding sites of relatively high affinity ; or the participation of receptors other than those involved in migration over plastic coated with FN , FGN or SF . . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
For all cell types tested , peptides effective at inhibiting fibronectin promoted cell spreading were found also to inhibit cell spreading promoted by human serum spreading factor ( SF ) . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
The findings indicate that the SF platelets had large amounts of surface bound and small amounts of intracellular fibronectin , whereas the converse was found in the case of peripheral platelets from the patients and normal subjects . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Fibronectin , a glycoprotein produced by mesenchymal cells , was present in 11 of 16 plasma cryoprecipitates and 12 of 14 synovial fluid ( SF ) cryoprecipitates . ^^^ Fibronectin levels were related to the development of serum turbidity in the cold and fibronectin was involved in the development of cold turbidity induced by some charged polysaccharides in plasma , serum , and SF . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
To determine if differential glycosylation of fibronectin ( Fn ) in inflammatory synovial fluid ( SF ) included expression of an oncofetal epitope ( Onf Fn ) heretofore detected only on Fn derived from embryonal or neoplastic tissue . ^^^ Fn were purified from plasma , SF and synoviocyte conditioned medium by affinity chromatography and were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis and Western blotting utilizing a monoclonal antibody ( FDC 6 ) specifically recognizing the Onf Fn . ^^^ The Onf Fn was not expressed on Fn isolated from normal or RA plasma but was strongly expressed on Fn from rheumatoid arthritis ( RA ) SF and to a lesser extent osteoarthritis SF . ^^^ Fn present in the SF but not plasma of patients with rheumatic disease contains an epitope previously thought to be restricted to Fn produced by embryonal or malignant tissue . . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
In Exp . 1 , SF was administered i . v . at 0 ( CONT ) , 50 ( LSF ) , 100 ( MSF ) , or 150 ( HSF ) microgram / kg . 75 BW in four mature Spanish cross does ( average BW 36 + / 7 kg ) fitted with indwelling jugular vein catheters in a 4 10 4 Latin square design . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
The highest SF binding capacities were observed for thrombospondin 1 ( TSP 1 ) , fibronectin ( Fn ) , and heparan sulfate proteoglycan , although SF did not bind to albumin . ^^^ Mature two chain SF and precursor single chain SF bound approximately equally well to TSP 1 and Fn . ^^^ Moreover , two SF alpha chain peptides ( NK 1 and NK 2 ) both bound to TSP 1 and Fn , suggesting that the whole SF molecule is not required for binding . ^^^ Based on binding competition assays , TSP 1 exhibited higher affinity for SF than did nine other ECM molecules , including Fn and heparan sulfate proteoglycan . ^^^ SF binding was modulated by binding interactions among ECM molecules ( TSP 1 Fn , TSP 1 collagen 1 , and Fn collagen 1 ) , suggesting that the matrix capacity to bind SF depends upon its exact composition . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
HGF / SF stimulated adhesion of the c MET+ B cell lines to the extracellular matrix molecules fibronectin ( FN ) and collagen ( CN ) in a dose dependent manner . ^^^ Adhesion to FN was mediated by beta 1 integrins alpha 4 beta 1 ( VLA 4 ) and alpha 5 beta 1 ( VLA 5 ) since blocking antibodies against beta 1 ( CD 29 ) , alpha 4 ( CD49d ) , or alpha 5 ( CD49e ) integrin subunits , completely reversed the effect of HGF / SF . ^^^ HGF / SF increased adhesion of c MET+ B lymphoma cells to FN and CN , mediated via beta 1 integrins alpha 4 beta 1 and alpha 5 beta 1 , and furthermore promoted migration and invasion . . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Furthermore , HGF / SF promotes adhesion of HPCs to immobilized fibronectin . ^^^ HGF / SF induced adhesion to fibronectin is probably caused by activation of the integrins alpha4beta1 and alpha5beta1 , insofar as we were able to block this interaction by using monoclonal blocking antibodies directed against these integrin subunits . ^^^ The enhanced adhesion of HGF / SF to fibronectin proved to be beneficial for the maintenance of the colony forming potential of HPCs . ^^^ HGF / SF alone and especially in combination with fibronectin prolongs survival of GM colony forming cells in liquid culture . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
In addition , reports identifying Fn fragments in synovial fluid ( SF ) were assessed . ^^^ RESULTS : A series of Fn fragments have been identified in arthritic SF by several investigators . ^^^ Fn and fragments ranging from 30 to 200 kd are present in elevated concentrations in inflammatory SF . ^^^ SF Fn fragments display reduced affinity for fibrin and collagen . ^^^ CONCLUSIONS : Fn fragments can be functionally and biochemically characterized in diseased SF . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Steel factor ( SF ) or Kit ligand is produced as a membrane bound form ( mSF ) and a soluble form . ^^^ Because both primary gynecological tumors and primary leukemic cells from patients with acute myeloblastic leukemia ( AML ) have been shown to coexpress c Kit and SF , we addressed the question of whether mSF could contribute to cell interaction in these cancers . ^^^ Using an antisense strategy with phosphorothioate modified oligonucleotides that specifically target SF without affecting other surface markers , we provide direct evidence for a role of mSF and c Kit in cell interaction and cell survival in these gynecological tumor cell lines as well as in primary AML blasts . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
OBJECTIVE : To characterize the species of synovial fluid ( SF ) fibronectin ( FN ) bearing the alternatively spliced EIIIA segment . ^^^ The absence of recognition of EIIIA sequences within the approximately 200+ kd forms of OA SF FN was unrelated to their derivation from dimers , since anti EIIIA mAb recognized the smaller fragment species in preference to both monomeric and dimeric forms . ^^^ Additionally , `` FN species specific ' ' recognition of this segment within OA SF could constitute a marker by which to gauge the activity of the OA disease process . . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
OBJECTIVE : To characterize the domain localization , quantitation , and functional binding consequences of an O linked oligosaccharide expressed on synovial fluid ( SF ) fibronectin ( Fn ) . ^^^ These SF collagen binding Fn fragments were more basic and had higher gelatin binding affinities than corresponding plasma fibronectin fragments . ^^^ METHODS : Identification and localization of the O linked oligosaccharide was performed by limited digestion of isolated SF Fn with a series of proteolytic enzymes followed by Western blotting with peroxidase labeled peanut agglutinin . ^^^ RESULTS : A desialyated O linked oligosaccharide was identified on the C terminal 18 kDa segment of the SF Fn collagen binding domain . ^^^ Expression of this O linked oligosaccharide was highest on Fn isolated from osteoarthritic SF , followed by Fn isolated from rheumatoid arthritis SF , and finally normal human plasma . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
The sitABCD feoB mutant ( mutant SF ) and the mntH sitABCD feoB mutant ( mutant MSF ) showed minimal Fe ( 2 ) uptake and were slightly impaired for replication in susceptible macrophages . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Collapse of statistical indices was defined as any of the following : pattern deviation probability plot without a single VF location showing P < 0 . 5 % ; corrected pattern standard deviation ( CPSD ) and pattern standard deviation ( PSD ) probability less than 5 % or within normal limits ( WNL ) ; short term fluctuation ( SF ) probability WNL ; glaucoma hemifield test ( GHT ) not outside normal limits ( ONL ) ; or presence of a low patient reliability comment triggered by 40 % or more false negative ( FN ) responses . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Interestingly , we found , using the calcium channel blocker , 2 APB ( 2 aminoethoxydiphenyl borate ) and Lyn / BMMCs that both IgE and IgE + Ag induced adhesion to FN require extracellular calcium entry , whereas SF does not . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
SF selectively induced migration towards fibronectin , but not towards collagen 1 . ^^^ SF reduced adhesion of NHK to collagen types 1 and 4 , laminins 1 and 5 and fibronectin . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
In the confluent , central cells in SF medium alpha5beta1 showed a punctate distribution while Fn was present in strongly staining fibres . ^^^ In the peripheral , motile cells of the patches in SF conditions alpha5beta1 was localized in well defined focal adhesion plaques at the ends of actin stress fibres , while Fn was distributed in a punctate perinuclear pattern . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Four face types were used , including original ( broad band SF , BSF ) , low SF ( LSF , < 5 cycles / face ) , middle SF ( MSF , 5 15 cycles / face ) , and high SF ( HSF , > 15 cycles / face ) face images . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
The RNA was hybridized with HGF , fibronectin , and alpha actin specific cDNA probes , consecutively . ^^^ The amount of the HGF transcripts decreases significantly in FSC during the time of culture , while fibronectin gene expression increases and alpha actin gene expression as well . ^^^ TGF beta dramatically inhibits HGF gene expression , but causes an enhanced fibronectin mRNA level . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
FN did not affect the migration of HGF , barely enhanced that of HABC , and was less potent than CAP at enhancing the migration of HPC . ^^^ FN was more effective than CAP in promoting the attachment of HGF to root slices , but it was as potent as CAP in supporting the attachment of HPC and HABC . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Treponema denticola adhesion and degradation of fibronectin ( Fn ) on human gingival fibroblasts ( HGF ) were studied by immunofluorescence and enzyme linked immunosorbent assays . ^^^ Anti human Fn impaired bacterial adhesion to HGF . ^^^ Sonicated extracts and diluted spent growth medium degraded HGF Fn but , unlike intact T . denticola cells , they hardly stimulated F actin rearrangements . . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
By individually adding back these components to MDCK cells grown in Type 1 collagen gels in the presence of HGF , we were able to establish that : ( 1 ) certain ECM proteins , such as laminin , entactin , and fibronectin , actually facilitated the formation of branching tubular structures and increased their complexity ; ( 2 ) other ECM proteins , such as Type 4 collagen , heparan sulfate proteoglycan , and vitronectin , caused marked inhibition of HGF induced morphogenesis ; and ( 3 ) not only did transforming growth factor beta ( TGF beta ) inhibit the formation of tubular structures , but those which did form exhibited little branching , thereby suggesting that TGF beta modulates tubulogenesis as well as branching . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Furthermore , the fact that vascular cell adhesion molecule 1 ( VCAM 1 ) , one of the ligands of VLA 4 , was not detected on HGF by flow cytometry and anti fibronectin ( FN ) Ab did not block the adhesive interaction to HGF suggests that not yet identified ligand ( s ) for VLA 4 might be present on HGF . . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Thus , both growth factors ( HGF and TGF beta ) and fibronectin may be important regulators of paracrine stimulation by stromal cells of anchorage independent growth of mammary carcinoma cells . . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
The effects of HGF on the synthesis of albumin , transferrin , fibronectin , alpha 1 antichymotrypsin , and haptoglobin could be counteracted by the simultaneous presence of IL 6 in the incubation media . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Increased proliferation and elevated production of the ECM molecules type 1 collagen and fibronectin ( FN ) could contribute to the clinical increased bulk of HGF gingiva . ^^^ All HGF strains produced greater amounts of FN ( measured by ELISA ) than all of the normal fibroblast strains ( 23 % to 49 % increase , depending on the strain ) ( P < or = 0 . 04 ) . ^^^ The results show that , in vitro , HGF fibroblasts display several phenotypic characteristics of activated fibroblasts : increased proliferative rates as well as increased production of FN and type 1 collagen , consistent with in vitro studies of fibroblasts derived from other types of fibrotic tissue . ^^^ These results suggest that the increased proliferation of HGF fibroblasts and their increased production of extracellular matrix molecules such as collagen and FN may contribute to the clinical gingival enlargement characteristics of HGF . . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Progressive PKD is associated with the cellular expression of proto oncogenes ( fos , myc , ras , erb ) , growth factors ( EGF , HGF , acid and basic FGF ) , chemokines ( MCP 1 . osteopontin ) , metalloproteinases , and apoptotic markers , and the interstitial accumulation of Types 1 and 4 collagen , laminin , fibronectin , macrophages and fibroblasts , the magnitudes of which increase with age . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Pretreatment with the proteinase inhibitor phenylmethylsulfonyl fluoride , which had previously been found to block T . denticola ' s degradation of endogenous fibronectin and detachment of HGF from the extracellular matrix , had little effect on F actin stress fiber disruption and the IP response . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
These data suggest that HGF increase the invasion activity of human HCC cell lines by affecting the production of u PA or the chemotaxis for fibronectin . . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
In vitro , HGF fibroblasts produce greater amounts of the ECM components fibronectin ( FN ) and type 1 collagen than normal human gingival ( GN ) fibroblasts . ^^^ The objective of this study was to determine whether HGF fibroblasts produce TGF beta and , with the use of neutralizing antibodies to TGF beta isoforms , if their increased expression of FN and type 1 collagen is under autocrine TGF beta control . ^^^ The HGF strains produced greater amounts of TGF beta 1 and TGF beta 2 ( P < or = 0 . 003 ) as well as FN ( P < or = 0 . 04 ) and type 1 collagen ( P < or = 0 . 03 ) ( measured by specific ELISA ) than the GN strains . ^^^ Treatment of HGF fibroblasts with anti TGF beta 1 , beta 2 , or beta 3 , as well as a combination of all 3 antibodies , decreased their FN production by up to 60 % ( P < or = 0 . 04 ) , and was able to decrease FN production by HGF fibroblasts to the levels of the GN fibroblasts . ^^^ The results suggest that autocrine stimulation by the increased amounts of TGF beta isoforms made by HGF fibroblasts contributes to their increased production of FN and type 1 collagen . . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Constitutive expression of HGF modulates renal epithelial cell phenotype and induces c met and fibronectin expression . ^^^ Autocrine expression of HGF and c met in renal epithelial cells also stimulated fibronectin gene expression , which was totally blocked by incubation with a neutralizing anti HGF but not a pan specific anti TGF beta antibody , suggesting that it is independent of TGF beta production . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Decreased fibronectin expression in Met / HGF mediated tumorigenesis . ^^^ We learned that Met / HGF signaling resulted in greatly decreased fibronectin mRNA production in three different human and mouse tumor cell lines ; these decreases in fibronectin mRNA were paralleled by decreases in fibronectin protein . ^^^ We also found a progressive decrease in fibronectin in tumor explants and metastases derived from the Met / HGF transformed cells . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
In this study we examined the effects of Porphyromonas gingivalis proteases on human gingival fibroblast ( HGF ) integrins and their fibronectin matrix . ^^^ However purified RI , an alpha / beta heterodimer in which the catalytic chain is associated with an adhesin chain , was 12 times more active than RIA , the catalytic monomer , in causing loss of the alpha5beta1 integrin ( fibronectin receptor ) from HGF . ^^^ It is concluded that the adhesin component of RI targets the P . gingivalis arginine protease to sites of fibronectin deposition on HGF , contributing to the rapid loss of both fibronectin and its main alpha5beta1 integrin receptor . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
In addition , HGF stimulated migration of early week 4 BMMC , but not of the later stage week 10 BMMC , on fibronectin and laminin substrates . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
We found that in invasive and metastatic MTLn 3 breast carcinoma cells , HGF stimulated both initial adhesion to and motility on the extracellular matrix ( ECM ) ligands laminin 1 , type 1 collagen , and fibronectin . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Functional HGF receptors were deed in MMC by HGF induced extracellular acidification , a response that was inhibited by the HGF inhibitor HGF / NK2 , a splice variant expressing the N terminal domain through the second kringle domain HGF also increased fibronectin and collagen alpha 1 ( 4 ) mRNA levels in these cells ; the increases were associated with a concentration dependent increase in transcriptional activity of the collagen alpha 1 ( 4 ) gene . ^^^ HGF also stimulated fibronectin and collagen alpha 1 ( 4 ) mRNA levels in primary rabbit proximal tubule epithelial cells To evaluate the potential consequences of chronic elevation of HGF on renal fuction , HGF was administered continuously for 18 days to normal and diabetic C57BLKS / J lepr ( db ) mice . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
On the other hand stimulation of c met receptor with HGF leads to enhanced integrin mediated adhesion of activated B cells to vascular cell adhesion molecule ( VCAM 1 ) and fibronectin . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Exogenous HGF expression dramatically inhibited alpha smooth muscle actin expression , attenuated renal interstitial accumulation and deposition of collagen 1 and fibronectin . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
HGF also blocked morphologic transformation of tubular epithelial cells and inhibited the expression and extracellular deposition of fibronectin . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
We used HA tagged SGK 1 to transiently transfect MDCK cells and study the regulation of SGK 1 upon stimulation with HGF , cAMP or upon adhesion of the cells to immobilized fibronectin . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
HGF significantly suppressed renal alpha smooth muscle actin expression , total kidney collagen contents , interstitial matrix components , such as fibronectin , and renal expression of transforming growth factor beta 1 and its type 1 receptor . ^^^ Delayed administration of HGF also suppressed the myofibroblastic transdifferentiation from tubular epithelial cells in vitro , as demonstrated by a decline in alpha smooth muscle actin and fibronectin expression . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
The chemoattractant activity was eluted at a molecular mass of around 600 kDa , which would include laminin and fibronectin , but not HGF , determined by ELISA . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
We investigated the effect of HGF on the invasion of PC 3 and DU 145 prostate cancer cells through a reconstituted basement membrane ( Matrigel ) , the haptotactic migration to fibronectin substrate , the expression of protein and mRNA for matrix metalloproteinases ( MMP ) 1 and 9 , membrane type 1 MMP ( MT 1 MMP ) , urokinase type plasminogen activator ( u PA ) and its receptor ( uPAR ) . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
At 0 , 24 , and 72 h after wounding , changes in mRNA expression of transforming growth factor beta 1 ( TGF Beta 1 ) , b FGF , HGF , VEGF , and FN were semiquantified by reverse transcription polymerase chain reaction . ^^^ For RPMC , TGF Beta 1 , HGF , b FGF , and FN mRNA gradually increased up to 72 h postwounding to 1 . 5 fold , 1 . 6 fold , 1 . 3 fold , and 2 . 1 fold of the control levels , respectively . ^^^ For RPFB , HGF , b FGF , VEGF , and FN mRNA expression were slightly suppressed compared to control levels up to 72 h postwounding . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
The HGF stimulated the production of hyaluronic acid ( HA ) and decreased the production of collagen type 1 from the fibroblasts in Reinke ' s space ( FbRS ) , whereas fibronectin production was not affected . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
In previous work , HGF fibroblasts grew faster and produced more collagen and fibronectin ( FN ) than normal gingival ( GN ) fibroblasts . ^^^ HGF FN and collagen production , but not proliferation , were under autocrine transforming growth factor ( TGF ) beta control , suggesting other means of activation of HGF proliferation . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Further studies on the mechanisms of DMCs to promote wound healing indicate that the supernatant of DMCs could promote the proliferation of fibroblasts and epidermal cells ; DMCs expressed transcripts of a series of cytokines and extracellular matrix molecules , including VEGF , PDGF , HGF , TGF beta , ICAM 1 , VCAM 1 , and Fibronectin , which were closely related to the wound healing by DNA microarray analysis . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Exogenous HGF also mitigated glomerular mesangial expansion , reduced fibronectin and type 1 collagen deposition , and prevented interstitial myofibroblast activation . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
EXPERIMENTAL DESIGN : Four biological sequels of HGF related to multiple myeloma were studied : ( 1 ) proliferation of myeloma cells , ( 2 ) secretion of interleukin 11 from osteogenic cells , ( 3 ) migration of myeloma cells , and ( 4 ) adhesion of myeloma cells to fibronectin . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
An adhesion assay revealed that treatment with TGF beta 1 , HGF , and RA induced a marked reduction of cell attachment to fibronectin ( FN ) coated surfaces , whereas there was no change with bFGF and EGF . ^^^ These findings suggest that TGF beta 1 , HGF , and RA may regulate pulpal cell adhesion to FN coated surfaces and that this effect is mediated by TN . . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
HGF induces fibronectin matrix synthesis in melanoma cells through MAP kinase dependent signaling pathway and induction of Egr 1 . ^^^ We report that hepatocyte growth factor / scatter factor ( HGF ) induces fibronectin expression and its extracellular assembly on the surface of melanoma cells through activation of mitogen activated protein ( MAP ) kinase pathway , and induction and transcriptional activation of Early growth response 1 ( Egr 1 ) . ^^^ Inhibition of B RAF / MAP kinase pathway by dominant negative mutants and by U 0126 abrogated HGF induced Egr 1 , and chromatin immunoprecipitation showed that Egr 1 is bound to the fibronectin promoter in response to HGF . ^^^ Exogenously expressed Egr 1 increased fibronectin levels , while blockage of Egr 1 activation by expression of the Egr 1 corepressor NAB 2 interfered with the upregulation of fibronectin synthesis induced by HGF , indicating that Egr 1 exerts a significant role in fibronectin expression in response to HGF . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
For the myofibroblast like cells , HGF increased activities of MMP 2 / 9 , predominant enzymes for breakdown of fibronectin ( FN ) . ^^^ Under such conditions , HGF induced caspase dependent apoptosis , linked with a decrease in a FN central cell binding ( CCB ) domain involved in FAK phosphorylation . ^^^ When MMI 270 ( a broad spectrum MMP inhibitor ) was added together with HGF , decreases in FN CCB domain expression and FAK phosphorylation by HGF were restored , and these events were associated with an inhibition of HGF induced apoptosis , suggesting that increased activities of MMPs underlie the major mechanism of HGF mediated apoptosis in myofibroblasts . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Compared with CCs of 1 week , PDLs and ABCs after 2 weeks revealed relatively high levels of all analyzed mRNAs , viz . , for EGF , HGF , VEGF , TGFbeta 1 , collagen 1 ( COL 1 ) , osteonectin ( ON ) , fibronectin ( FN 1 ) , and MMP 13 . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
The concentrations of EGF , TGF beta 1 , PDGF AB , FGF , HGF , fibronectin , vitamin A and vitamin E in these samples were evaluated with ELISA and HPLC . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Immunoreactive TGF beta 1 , connective tissue growth factor , HGF receptor , c Met , and fibronectin all increased in remnant ( 2 . 85 + / 0 . 51 , 3 . 83 + / 0 . 55 , 2 . 56 + / 0 . 31 , and 2 . 80 + / 0 . 39 fold sham respectively , n = 4 8 , p < 0 . 05 ) , and SC 58236 blunted the increases ( to 1 . 45 + / 0 . 34 , 1 . 85 + / 0 . 13 , 1 . 75 + / 0 . 30 , and 1 . 60 + / 0 . 32 fold sham ) . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
Incubation of cultured human coronary smooth muscle cells with human HGF ( 1 100 ng / ml ) did not significantly alter cell migration and collagen 1 , fibronectin , EMMPRIN , MMP 1 , MMP 2 and MMP 9 release . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
There was no statistically significant difference between HGF treated groups and control group in the result of alpha SMA immunostaining and the level of FN , except that CTGF mRNA expression was slightly increased in the HGF treated groups . ^^^ The addition of HGF inhibited the TGF beta 1 induced TEMT , the secretion of FN , and the CTGF expression of NRK52E cells , there was a significant correlation between the expression of CTGF and the expression of alpha SMA . ^^^ CONCLUSION : HGF could block TEMT and FN secretion triggered by TGF beta 1 , which implies that HGF could participate in renal interstitial fibrosis as a negative regulator . ^^^
Interacting proteins: P14210 and P02751 Pubmed SVM Score :0.0
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