Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.70526184
We tested a hypothesis that interactions between fibronectin ( FN ) , the major extracellular matrix component , and its integrin alpha 4 beta 1 receptor is important in the development of ischemia / reperfusion injury of steatotic liver transplants . 0.70526184^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.5799413
Our studies of T cell proliferation complement concurrent studies of T cell adhesion ; both demonstrate that resting CD4+ human T lymphocytes express the VLA integrins VLA 4 , VLA 5 , and VLA 6 , and can use these receptors to interact with the extracellular matrix ( ECM ) proteins fibronectin ( VLA 4 and VLA 5 ) and laminin ( VLA 6 ) . 0.5799413^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :1.4252625
These data suggest the wide use by plasma cells of VLA 4 , possibly as a ligand with fibronectin and high endothelial venules ( HEV ) . 1.4252625^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.97106748
These results indicate that myeloma cells use VLA 4 to interact with CS 1 / FN , H1 / FN and VCAM 1 on MM stroma , and that its function can be potentially up regulated , enabling higher degrees of cell adhesion to these VLA 4 ligands , which might influence myeloma cell localization in the bone marrow . . 0.97106748^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.70932341
We propose that surface associated FN is a ligand for VLA 4 , which by binding to VLA 4 on an adjacent cell , provides a costimulatory signal , thus sustaining T cell proliferation . . 0.70932341^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.51893489
Cell adhesion was reduced 95+ / 4 % by monoclonal antibodies HP1 / 2 and HP2 / 1 , which block VLA 4 binding to both VCAM 1 and FN connecting segment 1 . mAb HP1 / 3 preferentially blocked interaction of VLA 4 with FN but not VCAM 1 and decreased adhesion by 30+ / 8 % . 0.51893489^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
The leukocyte integrin alpha 4 beta 1 ( VLA 4 , CD49d / CD29 ) is a receptor for the extracellular matrix protein fibronectin and the endothelial adhesion protein VCAM 1 . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Role of integrin alpha 4 beta 7 / alpha 4 beta P in lymphocyte adherence to fibronectin and VCAM 1 and in homotypic cell clustering . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
The cell binding sites CS 1 and CS 5 in the IIICS region of human fibronectin ( FN ) mediate the adhesion of specific cell types by interacting with the integrin alpha 4 beta 1 . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Vascular endothelial cell adhesion and spreading promoted by the peptide REDV of the IIICS region of plasma fibronectin is mediated by integrin alpha 4 beta 1 . ^^^ This peptide has been reported to be the minimal active sequence within the CS 5 site of the alternatively spliced type 3 connecting segment ( IIICS ) region of fibronectin , and the integrin alpha 4 beta 1 has been identified as the receptor on melanoma cells for this site . ^^^ In this report we demonstrate for the first time that alpha 4 is present in the endothelial cell membrane , in contrast to previous reports by others , and that integrin alpha 4 beta 1 is the receptor for REDV mediated adhesion to the IIICS region of region of plasma fibronectin . . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
The CS 5 peptide is a second site in the IIICS region of fibronectin recognized by the integrin alpha 4 beta 1 . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Identification of a novel recognition sequence for the integrin alpha 4 beta 1 in the COOH terminal heparin binding domain of fibronectin . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Furthermore , CFU S 12 stem cells express the alpha 4 subunit of the VLA 4 integrin receptor , which is known to be a receptor for the CS 1 sequence , and monoclonal antibodies against the integrin alpha 4 subunit of VLA 4 block adhesion of CFU S 12 stem cells to plates coated with the C terminal fibronectin fragment . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Affinity chromatographic isolation of the melanoma adhesion receptor for the IIICS region of fibronectin and its identification as the integrin alpha 4 beta 1 . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Antibodies to integrin alpha 4 inhibit spreading of WEHI 231 cells on V+ fibronectin . ^^^ Therefore , integrin alpha 4 beta 1 is a fibronectin receptor specific for an alternatively spliced cell adhesion site and may play important roles in selective adhesion of various cell types to specific forms of fibronectin . . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Competitive binding of vascular cell adhesion molecule 1 and the HepII / IIICS domain of fibronectin to the integrin alpha 4 beta 1 . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Adhesion of committed human hematopoietic progenitors to synthetic peptides from the C terminal heparin binding domain of fibronectin : cooperation between the integrin alpha 4 beta 1 and the CD 44 adhesion receptor . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Integrin alpha 4 beta 1 mediated melanoma cell adhesion and migration on vascular cell adhesion molecule 1 ( VCAM 1 ) and the alternatively spliced IIICS region of fibronectin . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Integrin alpha 4 beta 1 recognizes both fibronectin ( CS 1 sequence ) and vascular cell adhesion molecule 1 ( VCAM 1 ) . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Mechanisms of VCAM 1 and fibronectin binding to integrin alpha 4 beta 1 : implications for integrin function and rational drug design . ^^^ Integrin alpha 4 beta 1 can mediate both cell cell and cell extracellular matrix adhesion by binding to either fibronectin or vascular cell adhesion molecule 1 ( VCAM 1 ) . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
The integrin alpha 4 beta 7 mediates lymphocytes adhesion to VCAM 1 on activated endothelium , fibronectin in the extracellular matrix , and the mucosal vascular addressin MAdCAM 1 . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Integrin alpha 4 beta 1 is a leukocyte receptor for fibronectin and vascular cell adhesion molecule 1 ( VCAM 1 ) . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Pre B cells adhere to fibronectin via interactions of integrin alpha 5 / alpha 5 with RGDS as well as of integrin alpha 4 with two distinct 5 region sequences at its different binding sites . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
In this report , we present evidence that cultured normal human fibroblasts express the integrin alpha 4 beta 1 and that this receptor facilitates fibroblast attachment to fibronectin . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Integrin alpha 4 beta 1 is a receptor for vascular cell adhesion molecule ( VCAM ) 1 and fibronectin ( CS 1 ) . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
The increase in integrin alpha 4 beta 1 expression on primary melanomas has been reported to significantly correlate with the development of metastases . alpha 4 beta 1 is a cell surface heterodimer that mediates cell cell and cell extracellular matrix interactions through adhesion to vascular cell adhesion molecule ( VCAM ) 1 and to the IIICS region of fibronectin . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Thus , tissue Fn contains approximately 50 % more of the CS 1 cell binding region recognized by the integrin alpha 4 beta 1 compared with plasma Fn . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
The novel recognition site in the C terminal heparin binding domain of fibronectin by integrin alpha 4 beta 1 receptor on HL 60 cells . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
The type 3 connecting segment of fibronectin contains an aspartic acid residue that regulates the rate of binding to integrin alpha 4 beta 1 . ^^^ The type 3 connecting segment ( IIICS ) within fibronectin is the major binding site for the integrin alpha 4 beta 1 . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
The integrin alpha 4 beta 1 interacts with the immunoglobulin superfamily member vascular cell adhesion molecule 1 ( VCAM 1 ) , and with an alternatively spliced form of fibronectin . ^^^ The integrin alpha 4 beta 7 binds not only VCAM 1 and fibronectin , but also the mucosal addressin cell adhesion molecule ( MAdCAM ) . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Integrin alpha 4 beta 7 mediates human eosinophil interaction with MAdCAM 1 , VCAM 1 and fibronectin . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
The lymphocyte integrin alpha 4 beta 1 is the receptor for the Hep 2 domain and CS 1 site in fibronectin ( Fn ) as well as for VCAM 1 . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Hematopoietic cells differentially bind to the C terminal heparin binding domain of fibronectin depending on the activation state of integrin alpha 4 beta 1 . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
FET cells expressed alpha 4 beta 1 integrin ( another fibronectin receptor ) ; however , addition of anti human integrin alpha 4 monoclonal antibody had no effect on DNA synthesis . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Recently we have shown an evidence that a peptide , corresponding to residues Gln 1892 to Gly 1910 , from the C terminal heparin binding domain of fibronectin promotes adhesion of phorbol 12 myristate 13 acetate ( PMA ) treated U 937 cells and binds to both integrin alpha 4 beta 1 and glycosaminoglycans on U 937 cells surface . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
LPAM 1 ( integrin alpha 4 beta 7 ) ligand binding : overlapping binding sites recognizing VCAM 1 , MAdCAM 1 and CS 1 are blocked by fibrinogen , a fibronectin like polymer and RGD like cyclic peptides . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
This sequence contains a tripeptide , IDG , homologous to the sequences LDV , IDA , and LDA in fibronectin and IDS in VCAM 1 recognized by the closely related integrin alpha 4 beta1 . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Similarly , avian embryos injected with synthetic peptides that contain the integrin alpha 4 binding site in fibronectin displayed abnormal neural crest cell migration . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Because integrin FN adhesive interactions have been shown to play essential roles in mammalian development , studies were initiated of integrin alpha 4 in amphibian embryos , which are better suited to experimental analyses of the earliest stages of embryogenesis . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
On the basement membrane of CNSDC , fibronectin , a ligand of integrin alpha 4 , was strongly and frequently expressed in PBC , while such expression on the biliary basement membrane was rare in other controls . ^^^ These results suggest that increased fibronectin expression on the biliary basement membrane and integrin alpha 4 fibronectin interaction facilitate adhesion and the penetration of infiltrating alpha 4 expressing lymphocytes into the biliary epithelial layer in PBC . . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
It is well established that integrin alpha 4 beta 1 binds to the vascular cell adhesion molecule ( VCAM ) and fibronectin and plays an important role in signal transduction . ^^^ To better understand how various inhibitors might block the interaction of VCAM and fibronectin with alpha 4 beta 1 , we began constructing a structure model for the integrin alpha 4 beta 1 complex . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Normal gastrula cells lack integrin alpha 4 and , correspondingly , are unable to adhere to the alpha 4 ligand , the 5 region of FN . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NAC pretreatment augmented integrin alpha 4 dependent fibronectin adhesion and aggregation of Jurkat cells without changing its expression by fluorescence activated cell sorter , suggesting that reduction of surface disulfides can affect proteins function . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Functional differences between integrin alpha 4 and integrins alpha5 / alphav in modulating the motility of human oral squamous carcinoma cells in response to the 5 region and heparin binding domain of fibronectin . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Lymphocytes and the H 2 tissue expressed adhesion molecule subunits for the following potential cell cell or cell matrix interactions : alpha 3 beta 1 ( VLA 3 ) to fibronectin , laminin , and collagen ; alpha 4 beta 1 ( VLA 4 ) and alpha 5 beta 1 ( VLA 5 ) to fibronectin ; alpha 6 beta 1 ( VLA 6 ) to laminin ; alpha 4 beta 1 to VCAM 1 ; alpha L beta 2 ( Leu CAMa / LFA 1 ) to CD 54 ( ICAM 1 ) ; CD 44 to fibronectin , collagen , laminin , hyaluronate ; CD 2 to CD 58 ( LFA 3 ) ; and CD 56 ( N CAM ) to CD 56 . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
In addition , the binding of T cells with the CS 1 alternatively spliced segment of fibronectin ( the binding site recognized by VLA 4 ) induced pp 105 tyrosine phosphorylation . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
These alpha 4 beta 7 positive JY cells bound poorly or not at all to VLA 4 ligands ( soluble form of vascular cell adhesion molecule 1 ( sVCAM 1 ) and the CS 1 region of fibronectin ) . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
A further enhancement of VLA 4 mediated T cell binding to VCAM 1 and fibronectin could be observed when already in vivo activated synovial T cells were stimulated in vitro with phorbol esters , suggesting the existence of several cellular affinity levels for both very late activation 4 ( VLA 4 ) ligands . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
This beta 1 mediated regulatory effect involved both VLA 4 and VLA 5 fibronectin receptors . ^^^ Non activated peripheral blood T lymphocytes , unable to mediate VLA 4 interactions with fibronectin or VCAM 1 , acquired the ability to bind these ligands in the presence of anti beta 1 mAb . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Combination of 4B9 mAb with function blocking antiserum to human fibronectin , a second known ligand for VLA 4 , also failed to inhibit as much as anti VLA 4 mAb . ^^^ These findings suggest that adhesion of lymphoid cell lines through VLA 4 or other alpha 4 integrins may involve inducible counter receptor ( s ) on endothelium distinct from either VCAM 1 or fibronectin . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
The role of VLA 4 fibronectin ( FN ) receptors in the interaction of activated SF T cells from RA patients with a 38 kD fragment of FN has been previously demonstrated . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Homogeneous marrow stromal cell monolayers capable of supporting proliferation of cocultivated CD34hi cells expressed VCAM 1 and fibronectin ( ligands for VLA 4 and VLA 5 ) as well as ICAM 1 ( ligand for LFA 1 and Mac 1 ) . ^^^ Adhesion blocking experiments indicated that VLA 4 / VCAM 1 , VLA 5 / fibronectin , and beta 2 integrin / ICAM 1 pathways all are important for CD34hi cell attachment to stromal cells . ^^^ In addition , CD34hi cells utilized VLA 4 to adhere to purified VCAM 1 and employed VLA 5 ( and to a lesser extent VLA 4 ) to adhere to purified fibronectin . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Finally , in adherence assays , Jurkat cell binding to resting FLS monolayers was inhibited by antibody to alpha 4 / beta 1 integrin ( VLA 4 ) , CS 1 peptide from alternatively spliced fibronectin ( which is another VLA 4 ligand ) , and , to a lesser extent , anti VCAM 1 antibody . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
The beta 1 integrin VLA 4 ( alpha 4 beta 1 , CD49d / CD29 ) , which is expressed on a large subpopulation of peripheral blood T lymphocytes , functions as a receptor for the endothelial adhesion protein VCAM 1 and the extracellular matrix protein fibronectin . ^^^ Previous studies showed that immobilized fibronectin enhanced anti CD 3 monoclonal antibody ( mAb ) induced T cell proliferation through binding to the integrins VLA 4 and VLA 5 ( alpha 5 beta 1 , CD49e / CD29 ) . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Phenotypical analysis of integrin receptor expression ( particularly FN receptors VLA 4 and VLA 5 ) at the membrane of untreated or PMA treated cells revealed that PMA induced no significant modification of the level of expression of these receptors . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
LFA 1 dependent adhesion was mediated by ligands on HEC other than the intercellular adhesion molecule 1 ( ICAM 1 ) and the VLA 4 related pathway was mediated by ligands other than the CS 1 domain of fibronectin . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Finally , pretreatment and coculture of the IM 9 cell line with interleukin 6 ( IL 6 ) resulted in a 52 % decrease in specific binding to fibronectin ( 30 % + / 6 % to 15 % + / 6 % ; P = . 001 ) , associated with a decrease in the number of cells expressing VLA 4 and a decrease in intensity of VLA 4 expression . ^^^ These data suggest that myeloma cells adhere to fibronectin through VLA 4 as well as through RGD dependent mechanisms , and that this binding can be downregulated by IL 6 . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
DN , DP , and SP subsets were isolated and examined for their capacity to express VLA 4 and to adhere to fibronectin ( FN ) molecules as well as thymic stromal cells expressing FN . ^^^ The binding of the DN subset to FN plates or thymic stromal monolayers was inhibited only marginally by the RGDS peptide , but was efficiently inhibited by V 10 peptide ( cell binding sequence that is located in the 5 region on FN and reacts with the VLA 4 integrin ) or anti VLA 4 antibody . ^^^ Anti VLA 4 antibody plus RGDS peptide strongly inhibited DN cell binding to FN coated plates and thymic stromal monolayers . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
VLA 4 fibronectin interaction is required for the terminal differentiation of human bone marrow cells capable of spontaneous and high rate immunoglobulin secretion . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Enhancement of CD 2 mediated T cell activation by the interaction of VLA 4 with fibronectin . ^^^ Evidence is provided that the interaction of VLA 4 with immobilized fibronectin can enhance the proliferation of T cells subjected to suboptimal stimulation via CD 2 . ^^^ Our results suggest that the fibroblast stimulated T cell proliferative response to low doses of anti T 112 and T 113 antibodies is due to the interaction of VLA 4 with fibroblast fibronectin . ^^^ These findings also suggest a role for the fibronectin / VLA 4 interaction in the inflammatory process . . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
VCAM 1 on activated endothelium interacts with the leukocyte integrin VLA 4 at a site distinct from the VLA 4 / fibronectin binding site . ^^^ In addition , VLA 4 expression allowed K 562 cells to attach to the heparin 2 binding region ( FN 40 ) of fibronectin . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
In order to ascertain whether these adhesive proteins are normally expressed in inherited epidermolysis bullosa ( EB ) dermal epidermal junction , we studied the reactivity of MoAb recognizing receptors for VLA 1 ( R for unknown ligand ) , VLA 2 ( R for collagen ) , VLA 3 ( R for collagen , laminin , fibronectin ) , VLA 4 ( R for unknown ligand ) , VLA 5 ( R for fibronectin ) , VLA 6 ( R for laminin ) , VNR alpha , and VNR beta ( R for vitronectin ) on cryostat skin sections from EB patients and normal controls and on cytospins of normal epidermal cell suspensions with indirect immunohistochemical methods . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Fibronectin promotes proliferation of naive and memory T cells by signaling through both the VLA 4 and VLA 5 integrin molecules . ^^^ The augmentation of anti CD 3 stimulated proliferation induced by immobilized fibronectin was also inhibited partially by mAb to either VLA 4 or VLA 5 and completely by a combination of the two mAb . ^^^ The mAb to VLA 4 not only blocked the capacity of immobilized fibronectin to enhance anti CD 3 induced T cell proliferation but also directly costimulated T cell responses . ^^^ These studies indicate that signals are transduced through the fibronectin receptors , VLA 4 and VLA 5 , that augment T cell responses and therefore implicate the extracellular matrix protein fibronectin as an important influence regulating T cell responsiveness in vivo . . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Human natural killer cells express VLA 4 and VLA 5 , which mediate their adhesion to fibronectin . ^^^ Very late Ag ( VLA ) 3 , VLA 4 , and VLA 5 , belonging to the beta 1 subfamily of integrins , have been recently identified as receptors for different binding regions of fibronectin ( FN ) . ^^^ Using as inhibitory probes a polyclonal antibody against the beta 1 chain of the human FN receptor , the synthetic peptide GRGDSP , which is able to inhibit cellular adhesion mediated by VLA 5 , the CS 1 fragment , which contains the principal adhesion site in the IIICS domain recognized by VLA 4 , and functional mAb directed against alpha 4 or alpha 5 subunits , we show that both VLA 4 and VLA 5 mediate the adhesion of human NK cells to FN . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
All cell lines expressed VLA 3 and VLA 4 , two other receptors reported to mediate fibronectin binding in some cell types . ^^^ In addition , Raji cells , which lack VLA 5 but express VLA 3 and VLA 4 , showed very low level binding to fibronectin . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
The human integrin VLA ( very late activation antigens ) 4 ( CD49d / CD29 ) , the leukocyte receptor for both the CS 1 region of plasma fibronectin ( Fn ) and the vascular cell surface adhesion molecule 1 ( VCAM 1 ) , also mediates homotypic aggregation upon triggering with specific anti VLA 4 monoclonal antibody ( mAb ) . ^^^ By testing this panel of anti VLA 4 mAb for inhibition of cell binding to both a 38 kDa Fn fragment containing CS 1 and to VCAM 1 , as well as for induction and inhibition of VLA 4 mediated homotypic cell adhesion , we have found overlapping but different functional properties associated with each epitope . ^^^ Further evidence for multiple VLA 4 functions is that anti Fn and anti VCAM 1 antibodies inhibited binding to Fn or to VCAM 1 , respectively , but did not affect VLA 4 mediated aggregation . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Although fibronectin is a ligand for VLA 4 , anti fibronectin antibody and a soluble fibronectin fragment containing the VLA 4 binding domain did not block adhesion , suggesting that VLA 4 is involved in adhesion of B cell precursors , but not as a fibronectin receptor . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
The different adhesive capacities of the VLA 4 integrin , namely the interaction with a 38 kDa fibronectin fragment containing the CS 1 region of plasma fibronectin ( Fn 38 ) , the binding to the vascular cell adhesion molecule 1 ( VCAM 1 ) , or the ability to mediate the anti alpha 4 induced cell aggregation , were not altered on VLA 4 from cells upon mild trypsin treatment , when compared to non treated cells . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Most cell lines showed coexpression of the fibronectin receptors ( VLA 4 and VLA 5 ) and the laminin receptor ( VLA 6 ) . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Transfectants with the intact or the cleaved alpha 4 were equally capable of engaging in VLA 4 dependent adhesion to vascular cell adhesion molecule 1 and to the Hep 2 fragment of fibronectin ( 40 kDa ) and aggregated equally well in response to anti alpha 4 antibodies . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Coexpression of two fibronectin receptors , VLA 4 and VLA 5 , by immature human erythroblastic precursor cells . ^^^ In this study , we report data showing that immature human erythroblasts express the integrins VLA 4 and VLA 5 and that both these molecules act as fibronectin receptors on these cells . ^^^ Furthermore , monoclonal antibodies recognizing the VLA alpha 4 chain blocked the adhesion of immature erythroblasts to Fn coated surfaces , thus demonstrating that , as VLA 5 , VLA 4 is also a functional Fn receptor on these cells . . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Its binding to FN was inhibited by anti FN antibody or a mixture of synthetic peptides corresponding to two different sites of FN , termed the V 10 sequence and the RGDS ( Arg Gly Asp Ser ) sequence , which interact , respectively , with the VLA 4 and VLA 5 FN receptors expressed on T lineage cells . ^^^ The involvement of FN FN receptor interaction in this adhesion was demonstrated by the following lines of evidence : ( 1 ) the MRL104 . 8a cells expressed FN molecules on their surface and ( 2 ) the adhesion of CD 4 8 thymocytes to MRL104 . 8a monolayers was almost completely inhibited by simultaneous addition of anti FN antibody and a mixture of peptides ( V 10 plus RGDS ) capable of binding to anti FN receptors ( VLA 4 and 5 ) . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Upregulated expression and function of VLA 4 fibronectin receptors on human activated T cells in rheumatoid arthritis . ^^^ We have investigated the expression and function of VLA 4 fibronectin ( FN ) receptors on T cells localized in the inflammed synovium of patients with rheumatoid arthritis ( RA ) . ^^^ Furthermore , the majority of these activated SF T cells were able to adhere to a 38 kD FN proteolytic fragment containing the connecting segment 1 ( CS 1 ) specifically through VLA 4 receptors , whereas a significantly lower proportion of PB T cells displayed this capacity . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
The induced expression of VLA 4 after activation of tonsillar B lymphocytes was accompanied by the acquisition of the capacity to bind to a 38 kDa proteolytic fragment , containing the connecting segment 1 domain , of FN . ^^^ Interestingly , nonactivated peripheral blood B cells were unable to attach to this FN fragment , in spite of their constitutive expression of VLA 4 , and only acquired this functional capacity after cell activation with phorbol esters and SAC . ^^^ These results underline that the FN binding activity of VLA 4 is dependent on processes affecting cellular activation as described for other members of the integrin family . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
VLA 4 binds to the peptide sequence Gly Pro Glu Ile Leu Asp Val Pro Ser Thr ( GPEILDVPST in single letter code ) on the alternatively spliced CS 1 form of FN , whereas VLA 5 binds to an Arg Gly Asp sequence found on all forms of FN . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
VLA 4 mediates CD 3 dependent CD4+ T cell activation via the CS 1 alternatively spliced domain of fibronectin . ^^^ In the present study , we showed that VLA 4 is also involved in this CD 3 dependent CD 4 cell activation through its interaction with the alternatively spliced CS 1 domain of FN . ^^^ These data indicate that VLA 4 mediates CD 3 dependent CD 4 cell proliferation via the CS 1 domain of FN . ^^^ Anti VLA 4 also partially ( 10 40 % ) inhibited CD 4 cell proliferation induced by native FN plus anti CD 3 , implying that the CS 1 domain is active in the native plasma FN . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Because the appropriate reagents that recognize individual mouse VLA proteins were not available , we used the human T cell line Jurkat to demonstrate that T cell binding to collagen , laminin , and fibronectin is mediated largely by VLA 2 , VLA 6 , and a combination of VLA 5 and VLA 4 , respectively . . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
We describe here the regulated binding of resting CD4+ human T cells to ECM through three VLA integrins : VLA 4 and VLA 5 binding to fibronectin ( FN ) , and a novel pathway of VLA 6 binding to laminin ( LN ) . ^^^ Second , binding is regulated with T cell differentiation ; memory T cells express three to four fold more VLA 4 , VLA 5 , and VLA 6 than do naive cells , and bind more efficiently through them to FN and LN . . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Cell attachment to types 1 and 4 collagen and to fibronectin was not affected by antibodies to VLA 4 and VLA 6 . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Immunophenotypic analysis of marrow blast cells revealed a significant predominance of the ' null ' phenotype in the CD 34 positive group , together with a strong expression of the VLA 4 and VLA 5 integrins ( fibronectin receptors ) . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
The beta 1 subfamily of integrins is thought to play an important role in both the adhesion / migration and proliferation / differentiation of T cells . beta 1 integrins can provide T cell costimulation through interaction of very late antigen ( VLA ) 4 ( VLA 4 ) ( alpha 4 beta 1 ) and VLA 5 ( alpha 5 beta 1 ) with the extracellular matrix protein fibronectin ( FN ) , or by VLA 4 binding to its cell surface ligand , vascular cell adhesion molecule ( VCAM ) 1 . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Eosinophils exposed to the eosinophil active cytokines recombinant human interleukin 3 ( rhIL 3 ) and granulocyte / macrophage colony stimulating factor ( rhGM CSF ) in addition to CR 3 , CR 4 , and CD 9 expressed the beta 1 integrins VLA 4 and to a lesser extent VL 5 ( both fibronectin receptors ) as well as the intercellular adhesion molecule ICAM 1 . ^^^ The presence of VLA 4 as well as VLA 5 on activated eosinophils was confirmed by demonstration of the formation of immune rosettes using antibody coated microspheres and by their attachment to fibronectin coated surfaces . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Additional inhibition experiments indicated that VLA 4 interacted with unstimulated endothelium by binding to VCAM 1 and , to a much lesser extent , fibronectin . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
These results suggest that perturbation of CD 9 on pre B cells promotes recognition of stromal cell fibronectin by VLA 4 and VLA 5 and implicates CD 9 as a novel regulator of inside out signaling relevant to B lymphopoiesis . . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Whereas high levels of expression of vascular cell adhesion molecule 1 ( VCAM 1 ) and fibronectin , ligands for VLA 4 , were detected in the stroma cells , the adhesion and development of the erythroid progenitor cells were partly inhibited by the blocking antibody against VCAM 1 . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Although the involvement of fibronectin ( coated to B cells via VLA 4 ) in VLA 5 mediated T cell adhesion to B cells is feasible , an anti fibronectin and a VLA 4 specific antibody had no blocking effect . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
In contrast , treatment of stromal cells with antibodies to vascular cell adhesion molecule or fibronectin ( ligands of VLA 4 and VLA 5 ) did not prevent leukemic cell migration . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
VLA 4 , VLA 5 , and VLA 6 activated with either mAbs or their natural ligands ( fibronectin , laminin , and vascular cell adhesion molecule 1 ) are able to transduce costimulatory signals in thymocytes activated via the CD 3 pathway , i . e . , enhancement of thymocyte proliferation , CD 25 and CD 69 expression , and IL 2 secretion . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Inhibition studies with monoclonal antibodies revealed that this binding involves the VLA 4 / VCAM 1 and VLA 5 / fibronectin ( FN ) , but not the VLA 4 / CS1 pathway . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
VLA 4 dependent migration across IL 1 beta stimulated endothelium was markedly inhibited only when mAbs to VCAM 1 were added in combination with peptides of fibronectin . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
In contrast , VLA 1 , VLA 2 , and VLA 6 integrins were not expressed significantly . mAb to VLA 3 , VLA 4 , and VLA 5 each inhibited by 70 % skin mast cell adhesion to fibronectin . mAb to VLA 3 nearly abolished mast cells adhesion to laminin , whereas anti VLA 4 and anti VLA 5 were ineffective . ^^^ Our data demonstrate that human skin mast cells spontaneously adhere to fibronectin and laminin , and that this adhesion is mediated by VLA 3 , VLA 4 , and / or VLA 5 integrins on these cells . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Beads that were coated with the fusion protein maltose binding protein FimD bound to adherent monocytes , and this binding was inhibited by soluble fibronectin or mAb against the alpha or beta chain of VLA 5 , while soluble collagen or mAb against VLA 4 , VLA 6 , CR 3 , or HLA class 2 had no effect . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NK cells bind to fibronectin and laminin using the beta 1 integrins VLA 4 and VLA 5 , and VLA 6 respectively . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Nevertheless , anti alpha 5 MoAb completely inhibited chemotaxis when it was added with anti alpha 4 in FR4ds , demonstrating a novel complementary role of VLA 5 toward VLA 4 in the chemotaxis induced by FN . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
However , 5 delta 1+ cells exhibited higher levels of VLA 5 that correlated with an increased adhesiveness to fibronectin and to a 120 kDa fibronectin fragment ( FN 120 ) that contains only the VLA 5 binding domain but not to type 1 collagen or to a fibronectin fragment ( FN 40 ) that binds only VLA 4 . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Monoclonal antibodies to the alpha chain of VLA 4 , and the alpha chain of LFA 1 , as well as the synthetic peptide CS 1 ( which corresponds to the alternatively spliced connecting segment of fibronectin ) and the control peptide GRADSP , had no inhibitory effect on monokine production . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Cultured on FN resulted in the increment of the expressive CR 3 ( CD11b ) ( BSA vs FN ; MFI ratio 10 . 50 vs 12 . 09 p < 0 . 05 ) , and decrement of the expressive VLA 4 ( CD49d ) on the eosinophils ( 3 . 93 vs 2 . 91 ( 4 h ) p < 0 . 05 , 2 . 86 vs 2 . 09 ( 48 h ) p < 0 . 05 ) . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Moreover , we have demonstrated that the solid phase cross linking of VLA 4 using Ab ( against A and B ) or the CS 1 region of fibronectin , stimulated tyrosine phosphorylation of 140 , 120 , 80 to 70 , 60 to 55 , 50 , and 45 kDa proteins in addition to the 105 kDa protein . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
We examined the effects of a stimulatory anti beta 1 mAb ( TS2 / 16 ) and different divalent cations on VLA 4 mediated cell adhesion to vascular cell adhesion molecule 1 ( VCAM 1 ) , to the fibronectin derived CS 1 peptide , and to larger fibronectin fragments . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Only J11d+ thymocytes ( 12 to 15 % ) bound to fibronectin ( via VLA 4 and VLA 5 ) and binding to laminin ( via VLA 6 ) was two to fourfold higher in J11d+ compared with J11d thymocytes ( 60 to 80 % vs 20 to 30 % ) . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
The alpha 4 beta 1 integrin VLA 4 , expressed on pre B and mature / activated B cells , mediates adhesion of these cells to its two ligands , VCAM 1 and fibronectin . ^^^ We found that cross linking of VLA 4 by either mAb or natural ligands ( i . e . , VCAM 1 and the FN 40 cleavage fragment of fibronectin ) induced the tyrosine phosphorylation of a 110 kDa protein in a human pre B cell line ( Nalm 6 ) , an EBV transformed B cell line ( SB ) , and normal tonsillar B cells . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Similar activation of T cells with fibronectin also resulted in increased B 7 responsiveness , suggesting the involvement of VLA 4 molecule . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Studies with alpha subunit ( CD 49 ) specific mAbs indicated that the integrins VLA 4 and 5 mediated migration across FN preincubated filters , and VLA 2 , 4 , 5 , and 6 were involved in eosinophil migration through filters covered with HUVEC . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Upon stable transfection of wild type alpha 4 , VLA 4 heterodimer was obtained that mediated ( a ) poor adhesion to CS 1 peptide , fibronectin , or vascular cell adhesion molecule 1 ( VCAM 1 ) ( in K 562 cells ) ; ( b ) poor adhesion to CS 1 peptide but moderate adhesion to VCAM 1 ( in MIP 101 cells ) ; and ( c ) moderate adhesion to both CS 1 peptide and VCAM 1 ( in PMWK cells ) . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Both VLA 4 and VLA 5 fibronectin ( FN ) , as well as VLA 2 collagen ( COL ) receptors mediated cell spreading and morphological changes . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Analysis of binding mechanisms indicated that very late antigen 4 ( VLA 4 ) and VLA 5 were responsible for AML cell binding to fibronectin . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
VLA 4 integrin mediates lymphocyte migration on the inducible endothelial cell ligand VCAM 1 and the extracellular matrix ligand fibronectin . ^^^ We herein tested the ability of lymphocytes to utilize the beta 1 integrin VLA 4 to mediate cell migration when adhering to its cytokine inducible endothelial cell ligand VCAM 1 or its extracellular matrix ligand fibronectin . ^^^ Like the beta 2 integrin LFA 1 , VLA 4 may be a locomotive adhesion receptor which is involved in the transendothelial migration of lymphocytes and the infiltration of lymphocytes into lymphoid or peripheral tissues by binding to VCAM 1 and fibronectin . . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Migration was not inhibited by blocking either of the known VLA 4 counterreceptors ( VCAM 1 or fibronectin ) , although slight inhibition was observed using a combination of blocking antibodies to VCAM 1 and fibronectin . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
This FN enhancement of beta hexosaminidase release was inhibited by soluble MR alpha 4 1 , HM alpha 5 1 and HM beta 3 1 as well as by GRGDSP and DELPQLVTLPHPNHLGPEILDVPST peptides which abrogate VLA 5 / VNR and VLA 4 binding to FN respectively . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
We hypothesized that cellular fibronectin plays a pivotal role in the progression of the allograft arteriopathy by directing the transendothelial trafficking of inflammatory cells through interaction of the connecting segment 1 ( CS 1 ) motif with the very late antigen 4 ( VLA 4 ) integrin , and tested this in vivo using a blocking peptide . ^^^ Our data suggest that the VLA 4 fibronectin interaction is critical to the progression of the allograft arteriopathy by perpetuating the immune inflammatory response in the vessel wall . . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Both the random and chemotactic migration were dependent on fibronectin receptors VLA 4 and VLA 5 on T cells . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Cell adhesion to fibronectin before and after treatment with BZ alpha GalNAc or TPA was inhibited by anti VLA 4 and anti VLA 5 monoclonal antibodies . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Adhesion to fibronectin primes eosinophils via alpha 4 beta 1 ( VLA 4 ) . ^^^ From these studies we conclude that ( 1 ) alpha 4 beta 1 ( VLA 4 ) integrin is a major receptor for Fn on human eosinophils and ( 2 ) adhesion to Fn may prime eosinophils for mediator release during allergic inflammation . . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Parallel analysis of other integrin subunits mRNA ( beta 1 , alpha 5 , beta 7 ) demonstrated that the mRNA levels for the alpha subunits of the fibronectin receptors alpha 4 / beta 1 ( VLA 4 ) and alpha 5 / beta 1 ( VLA 5 ) are differentially regulated during the monocytic differentiation of myeloid cell lines , and suggested that myeloid cells express a heterodimer formed by the association of beta 7 with an integrin alpha subunit distinct from alpha 4 . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Augmentation of stimulated eosinophil degranulation by VLA 4 ( CD49d ) mediated adhesion to fibronectin . ^^^ We examined the effect of VLA 4 mediated adhesion to purified fibronectin ( FN ) on the stimulated release of the granular protein , eosinophil peroxidase ( EPO ) , in human peripheral blood eosinophils . ^^^ Treatment of eosinophils during incubation in FN coated wells with the anti VLA 4 monoclonal antibody HP2 / 1 attenuated stimulated EPO secretion caused by 10 ( 6 ) M FMLP+CYTB from 497 + / 40 to 285 + / 26 ng / 10 ( 6 ) eosinophils ( P < 0 . 02 ) . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Flow cytometric analysis showed that both peripheral blood lymphocytes and the LAK cells express fibronectin receptors VLA 3 , VLA 4 and VLA 5 on their surfaces . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
We also found that pre B cells lacking HSA did not bind to the FN 40 fragment of fibronectin but reexpression of HSA restored VLA 4 mediated binding to fibronectin . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
In particular , beta 1 subfamily integrins ( VLA 1 6 proteins ) have been found to mediate cell adhesion to extracellular matrix ( ECM ) component such as fibronectin , collagen , laminin ; however , VLA 4 has been found to exhibit both cell cell and cell matrix adhesion functions . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Stimulation of PBK 101 by ECM was not inhibited by monoclonal anti VLA 4 or polyclonal anti fibronectin antisera . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Double negative T cell contact with TC 1S cells also increased the production of fibronectin ( FN ) by the thymic stroma and the expression of the VLA 4 FN receptor on the DN cells . ^^^ Together these data suggest that FN / VLA 4 interaction may be an important component of stromal cell dependent thymocyte phenotypic differentiation and that this interaction can be one of the targets for the influence of RA in T cell development . . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
This study was performed to determine whether VLA 4 also affects lymphocyte adhesion to inflamed synovium through interaction with the alternatively spliced CS 1 domain of fibronectin . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Previous studies show that human myeloma derived cell lines specifically adhere to fibronectin ( FN ) through very late antigen 4 ( VLA 4 ; alpha 4 beta 1 integrin complex ) and RGD peptide mechanisms , which may contribute to the localization of tumor cells in bone marrow ( BM ) . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
VLA 4 dependent binding to fibronectin ( FN ) and to a human vascular cell adhesion molecule ( hVCAM 1 ) transfected murine cell line was measured using U 937 cells and guinea pig ( GP ) bronchoalveolar lavage ( BAL ) cells . ^^^ A species cross reactive , blocking monoclonal antibody directed against human VLA 4 ( TY 21 . 6 ) inhibited U937 / FN binding by 71 + / 7 % . ^^^ A specific VLA 4 blocking monoclonal antibody , TY 21 . 6 , inhibited U 937 and BAL cell binding to hVCAM 1 , but only inhibited FN binding with U 937 cells . . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Normal as well as malignant plasma cells were found to be strongly positive for the intercellular adhesion molecule ICAM 1 , the fibronectin receptor VLA 4 and the lymphocyte homing receptor CD 44 . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Antibodies to CD11a , CD 18 , VLA 4 alpha , and VLA 5 alpha failed to inhibit binding of AML cells to bone marrow fibroblasts but anti VLA 5 alpha antibodies inhibited AML cell binding to fibronectin by approximately 50 % . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Furthermore , the fact that vascular cell adhesion molecule 1 ( VCAM 1 ) , one of the ligands of VLA 4 , was not detected on HGF by flow cytometry and anti fibronectin ( FN ) Ab did not block the adhesive interaction to HGF suggests that not yet identified ligand ( s ) for VLA 4 might be present on HGF . . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
In view of the known binding of VLA 4 and VLA 5 to extracellular fibronectin ( FN ) , the adhesion of leukaemic cells to FN was evaluated in a colorimetric assay . ^^^ These studies indicated that ALL cells adhere to soluble fibronectin predominantly through the VLA 5 molecule ( blockable with the PHM 2 antibody and a peptide containing the RGD sequence ) although binding mediated by VLA 4 was also apparent in some experiments ( blockable by a 40 kDa fragment containing the heparin binding domain of FN and inhibitory antibodies ) . ^^^ These results indicate that precursor B ALL cells may adhere to marrow stroma through interaction of VLA 4 and VLA 5 with FN , although other mechanisms of adhesion may be important . . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
These interactions of the lymphocytes with 3H labelled FN in solution could be almost completely blocked by monoclonal anti VLA 4 and VLA 5 antibodies . ^^^ Although both freshly purified and MLC activated lymphocytes have equal expression of the integrins VLA 4 and VLA 5 , only activated cells are capable of ' spontaneous ' binding FN in solution via an integrin mediated process , probably via an increase in the affinity of these receptors for FN . . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
These peptides blocked the interaction of the integrins VLA 4 and VLA 5 with fibronectin ( FN ) , and our results suggested that by preventing the interaction of T cell integrins with FN , we successfully prevented the migration of T cells to sites of antigenic challenge . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Finally , mAb specific for the alpha 4 and alpha 5 subunits of the two major Fn binding molecules of macrophages ( VLA 4 and VLA 5 , respectively ) , either alone or in combination , were poor inhibitors of T . cruzi uptake , suggesting that several members of the VLA family , including VLA 4 and VLA 5 , are involved in binding and entry of T . cruzi into macrophages . . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Fn induced eosinophil survival was comparable to that obtained with exogenous interleukin 3 ( IL 3 ) or granulocyte / macrophage colony stimulating factor ( GM CSF ) and was inhibitable by antibodies against Fn , very late antigen 4 ( VLA 4 ) , IL 3 , and GM CSF . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Cortical thymocytes , identified as CD3dim cells by using immunofluorescence in suspension , are highly positive for VLA 4 , a fibronectin ligand . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Fibronectin receptors , VLA 4 or VLA 5 , were expressed at various levels on all myeloma cell lines . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
As assessed by inhibition of adhesion using mAb to the VLA 2 , 3 , 4 , 5 , and 6 , NK cell adhesion to fibronectin was mediated by VLA 4 and 5 , and their adhesion to laminin by VLA 3 and 6 . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Adhesion and costimulation of proliferative responses of human gamma delta T cells by interaction of VLA 4 and VLA 5 with fibronectin . ^^^ The very late antigens , VLA 4 and VLA 5 belong to the beta 1 subfamily of integrins and have been identified as receptors for different binding regions of fibronectin ( FN ) . ^^^ Most FN binding is mediated by VLA 4 . ^^^ Additionally , resting gamma delta T cells cultured on coimmobilized anti TCR delta 1 mAb and FN or the 40 kDa fragment ( which contains the adhesion site in the IIICS domain recognized by VLA 4 ) for 96 h in the absence of exogeneous IL 2 showed significant increase in proliferation when compared to that of resting gamma delta T cells cultured on immobilized anti TCR delta 1 mAb alone . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Adhesion assay to fibronectin revealed augmented adhesion of mononuclear cells in IgA nephropathy ( P < 0 . 05 ) , and this increased adhesion was inhibited by mAbs to VLA 4 alpha and 5 alpha . ^^^ The expression of beta 1 integrins in IgA nephropathy was similar to that of healthy individuals , but the function of these molecules in terms of adhesion to fibronectin though VLA 4 and VLA 5 is increased in these patients . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
This adherence was partially inhibited by antibodies specific for plasma FN , VLA 4 , VLA 5 , and VLA beta 1 , and by the synthetic peptide RGDS . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
The VLA 4 ( alpha 4 beta 1 ) integrin is involved in the adhesion of cells to fibronectin and vascular cell adhesion molecule 1 ( VCAM 1 ) . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
It is generally accepted that the interaction of cells with FN is mediated by several integrins such as alpha 5 beta 1 ( VLA 5 ) or alpha 4 beta 1 ( VLA 4 ) , and analysis of flow cytometry showed that 185 4 cells express these integrins on their surface . ^^^ Furthermore , these effects of FN are blocked specifically by RGD peptide or anti VLA 4 mAb . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
The MCP 1 induced binding of T lymphocytes to FN was rapid , dose dependent , and resulted from activation of both VLA 4 and VLA 5 . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
The structural characterization of FNHLs and reactive lymph nodes was studied with indirect immunohistochemical stainings using monoclonal antibodies against VLA 4 , VCAM 1 , and fibronectin , with special reference to the reaction pattern in the normal and neoplastic follicles . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Immunogold labelingsoliduselectron microscopy analysis revealed that the areas of contact between lymphoblasts and fibroblasts contained beta 1 integrins ( VLA 4 and VLA 5 ) , fibronectin , vascular cell adhesion molecule ( VCAM 1 ) , and a cartilage link protein , CD 44 . ^^^ Double immunogold labeling studies disclosed a direct in situ relationship between fibronectin and VLA 4 , VLA 5 , and CD 44 . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Cell adhesion and migration are regulated at distinct stages of thymic T cell development : the roles of fibronectin , VLA 4 , and VLA 5 . ^^^ We demonstrated previously that immature CD3loCD69lo double positive human thymocytes adhere avidly to FN via the integrin , VLA 4 . ^^^ Our studies identify a novel VLA 4 and VLA5 / FN mediated pathway likely to be involved in regulating cell traffic between the cortex and medulla of the thymus . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
We have previously shown that normal CD34+ HPCs express high levels of fibronectin ( Fn ) receptors very late antigen 4 ( VLA 4 ) and VLA 5 in a low affinity state , which do not allow HPCs to strongly adhere on immobilized Fn , and that cytokines such as interleukin 3 , granulocyte monocyte colony stimulating factor , and stem cell factor transiently activate these receptors , providing HPCs with an adhesive phenotype on Fn . ^^^ Therefore , we propose a 2 step model with an initial activation of VLA 4 and VLA 5 generated by cytokine receptors that is followed by a secondary signal resulting from Fn binding to VLA 4 and VLA 5 , which may cooperate with those generated by cytokine receptors . . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Controlled detachment assays in shear flow suggested that adhesive strength of VLA 4 for VCAM 1 or the 40 kD fragment of fibronectin ( FN 40 ) is more rapidly increased and subsequently reduced by MCP 1 than by MIP 1 alpha , and confirmed late and sustained activation of the adhesive strength of VLA 5 for the 120 kD fragment of fibronectin ( FN 120 ) . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
These results suggest that : 1 ) B cell activation may cause decreased expressions of VLA 4 and 5 , which gives a costimulatory effect on B cell activation itself again in cooperation with FN , 2 ) Increased expressions of VLA 4 and 5 on tonsillar lymphocytes with age may be related to regional immune response of the palatine tonsils . . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
We also showed that the solid phase crosslinking of VLA 4 by the antibody against this molecule or by its ligand , the CS 1 region of fibronectin , stimulates tyrosine phosphorylation of 140 , 120 105 , 80 70 , 60 55 , 50 and 45 kilodalton proteins . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Adhesiveness of VLA 4 ( alpha 4 beta 1 , CD29 / CD49d ) for vascular cell adhesion molecule 1 or fibronectin was rapidly increased but subsequently reduced by RANTES , MCP 3 , or C5a . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Together with mAb R 1 2 , we characterized the expression of VLA 4 ( alpha 4 beta 1 ) and VLA 5 integrins , the two major fibronectin receptors , on two long term cultured mast cell lines , CFTL 15 and MC / 9 . ^^^ During the first 3 weeks the expression of VLA 4 and VLA 5 increased progressively and both were involved in mediating adhesion of BMMC to fibronectin . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
The VLA 4 integrin in these cells was constitutively active in terms of adhesiveness to both fibronectin and vascular cell adhesion molecule 1 ( VCAM 1 ) . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
V 120 FN protein , an isoform that mediates the attachment of leukocytes through the VLA 4 integrin , was present in the mesangium and glomerular capillary loops in control animals , and increased in the severe phase of nephritis , coinciding with a strong leukocyte infiltration . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
To this end , we ( Adams , Lin , Lobb , and Gill , unpublished data ) and others have generated peptidomimetic small molecule antagonists of VLA 4 based on the connecting segment 1 ( CS 1 ) peptide sequence of fibronectin that are potent blockers of integrin adhesive function in vitro and show efficacy in vivo . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Eosinophil VLA 4 binding to fibronectin augments bronchial narrowing through 5 lipoxygenase activation . ^^^ Styrene microplate wells were FN coated and eosinophils were allowed to adhere in the presence of 1 ) buffer control , 2 ) 20 micrograms / ml monoclonal antibody ( HP2 / 1 ) to the alpha 4 beta 1 ligand ( VLA 4 ) on the eosinophils , 3 ) 20 micrograms / ml anti CD 18 R15 . 7 , 4 ) 20 micrograms / ml anti CD 16 3G8 , or 5 ) 10 ( 6 ) M A 63162 , a 5 lipoxygenase inhibitor . ^^^ Both augmented airway luminal narrowing and increased LTC 4 secretion caused by PAF activated cells after FN ligation were blocked completely by anti VLA 4 MAb ( P < 0 . 05 vs . control ) . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
VLA 5 in the plasma cell line , FR4ds , acts as a common regulator of VLA 4 and VLA 6 in spreading induced by fibronectin and laminin . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Stimulation of monocyte TFA may be more dependent on interactions between monocytes and the vegetational surface via fibronectin receptors , such as VLA 4 and VLA 5 ( very late antigens 4 and 5 ) . . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Among these ligands we could find fibronectin and VCAM 1 for VLA 4 , collagen 1 for VLA 2 and VLA 3 and laminin for VLA 2 , 3 and 6 . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Adhesion to FN was mediated by beta 1 integrins alpha 4 beta 1 ( VLA 4 ) and alpha 5 beta 1 ( VLA 5 ) since blocking antibodies against beta 1 ( CD 29 ) , alpha 4 ( CD49d ) , or alpha 5 ( CD49e ) integrin subunits , completely reversed the effect of HGF / SF . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
VLA 4 bearing lymphocytes conspicuously increased in number among the perivascular leukocytes , while immunoreactivity for LFA 1 , VCAM 1 , ICAM 1 and fibronectin was unchanged from that for post challenge day 2 . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
The alpha 4 beta 1 integrin very late activation antigen 4 ( VLA 4 ) has been implicated to play a role in the adhesive interactions between hematopoietic progenitor cells ( HPC ) and bone marrow stromal cells which express the vascular cell adhesion molecule 1 ( VCAM 1 ) or produce fibronectin ( FN ) . ^^^ Indeed , it has been shown that tyrosine kinase dependent stimulation of CD34+ hematopoietic cell lines with c kit ligand ( KL ) , IL 3 or GM CSF transiently activates the ability of VLA 4 to bind to VCAM 1 or FN . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Monocyte adherence to fibronectin or engagement of VLA 4 has been demonstrated to stimulate production of potent inflammatory mediators such as tumor necrosis factor alpha , interleukin 1 , and the procoagulant tissue factor protein . ^^^ Inhibition of tyrosine kinase activity with genistein ( 10 microg / ml ) as well as selective MAP kinase inhibition with the MEK 1 inhibitor PD 98059 abolished the VLA 4 dependent ERK tyrosine phosphorylation , inhibited NF kappaB nuclear binding , and abrogated tissue factor expression induced by both VLA 4 cross linking and adhesion to fibronectin in THP 1 cells and human peripheral blood monocytes . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
In vitro the integrin VLA 4 mediates the adhesion of haemopoietic progenitors to bone marrow stroma through an interaction with its ligands VCAM 1 and the CS 1 moiety of fibronectin . ^^^ These studies therefore imply that , in contrast to VCAM 1 , the CS 1 moiety of fibronectin is not a significant ligand in VLA 4 mediated progenitor trafficking in vivo . . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Binding of human leukocytes to fibronectin is augmented by an anti CD 44 mAb ( TL 1 ) and blocked by another anti CD 44 mAb ( Hermes 3 ) but not by anti VLA 4 / VLA 5 mAbs . ^^^ Lymphoid cells are activated by binding to FN of the VLA 4 and VLA 5 receptors . ^^^ This augmentation was not inhibited by the CS 1 and RGD peptides of FN or by anti VLA 4 and VLA 5 mAbs ; it was energy dependent and associated with cytoplasmic actin filaments . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Treatment of PMNL with a peptide based on the VLA 4 binding domain in the CS 1 fragment of fibronectin , but not a control peptide , inhibited PMNL migration to a comparable extent to treatment with mAb to VLA 4 . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
The CS 1 peptide of fibronectin ( which recognizes VLA 4 ) , when used in concert with anti CD 18 and anti VCAM 1 ( but not anti VLA 4 ) , caused a small , but significant increase in inhibition . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Transmigrated T cells exhibited downregulation of VLA 4 and LFA 1 integrin surface expression and decreased binding to rVCAM 1 and rICAM 1 and increased binding to collagens 1 and 4 , fibronectin , and laminin . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Human peripheral blood eosinophils express a functional c kit receptor for stem cell factor that stimulates very late antigen 4 ( VLA 4 ) mediated cell adhesion to fibronectin and vascular cell adhesion molecule 1 ( VCAM 1 ) . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
The enhanced adhesion of L 5 cells to fibronectin , laminin and Matrigel coated substrates , as well as their haptotactic migration to fribronectin , may be associated with the preferential expression of VLA 2 and VLA 4 integrins on the surface of these cells detected by flow cytometry . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Anti VLA 5 ( CD49e ) but not anti VLA 1 through VLA 4 and VLA 6 , blocked the effect of Fn on TNF alpha receptors , whereas anti VLA 6 but not anti VLA 1 through VLA 5 blocked the effect of Ln on IL 1 beta receptors . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Fibronectin binding was mediated via alpha4beta1 integrin ( VLA 4 ) , which is expressed at high levels on thymocytes . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
To further test this hypothesis , we administered by aerosol either a potent small molecule inhibitor of VLA 4 , which prevents VLA 4 mediated binding to fibronectin ( CS 1 ligand mimic ) , or an inactive control ( 30 mg twice daily for 3 d , and on the fourth day 0 . 5 h before and 4 h after antigen challenge ) to six sheep with airway hypersensitivity to Ascaris suum antigen . ^^^ The active agent , but not the inactive control , significantly blocked macrophage adherence to fibronectin ( FN ) , indicating that the CS 1 ligand interfered with VLA 4 mediated adhesion in sheep cells . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
The development of efficient culture techniques for the production of megakaryocytes ( Mks ) from CD34+ purified BM cells , enables the study of the expression and function of adhesion receptors for collagen ( VLA 2 ) , fibronectin ( VLA 4 and VLA 5 ) and laminin ( VLA 6 ) during the maturation of Mks . ^^^ Our data suggest that VLA 4 may be involved in the adhesion of CFU MK and immature Mks on fibronectin , then replaced by VLA 5 in the final stages of maturation . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Alternatively spliced CS 1 fibronectin isoform and its receptor VLA 4 in rheumatoid arthritis synovium . ^^^ Interaction between the integrin very late antigen 4 ( VLA 4 ) and the connecting segment 1 ( CS 1 ) fibronectin ( FN ) isoform may contribute to lymphocyte interaction in RA synovium . ^^^ We examined both mRNA and protein expression of CS 1 FN in inflamed synovium , and VLA 4 expression in synovial tissue and on cultured fibroblast like synoviocytes from patients with RA . ^^^ Thus , VLA 4 / CS 1 FN interaction may facilitate lymphocyte interaction and synovial proliferation in RA . . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Incubation with interleukin 6 and stem cell factor enhanced the expression of fibronectin ligands VLA 4 and VLA 5 on CD34+ cells . ^^^ This enhanced expression of VLA 4 and VLA 5 was considered to facilitate a close contact of the CD34+ cell to the retroviral vector via fibronectin fragments and the subsequent transduction process . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Fibronectin ( FN ) is supposed to play important roles in various aspects of hematopoiesis through binding to very late antigen 4 ( VLA 4 ) and VLA 5 . ^^^ Fibronectin ( FN ) is supposed to play important roles in various aspects of hematopoiesis through binding to very late antigen 4 ( VLA 4 ) and VLA 5 . ^^^ The augmented colony formation by FN was completely abrogated by the addition of CS 1 fragment , but not of GRGDSP peptide , suggesting an essential role of FN VLA 4 interaction in the FN effects . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Gene transfer was predominantly augmented by the interaction between VLA 4 on the T lymphocytes and the FN adhesion site CS 1 . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
The differential use of VLA 4 and VLA 5 correlated with an increase in fibronectin surface expression by stromal cells in response to TGF beta 1 . ^^^ Adhesion assays to purified fibronectin using Nalm 6 cells showed a predominant utilization of VLA 4 at low concentrations of this ligand , whereas higher concentrations resulted in a preferential use of VLA 5 . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
A novel region of the alpha 4 integrin subunit with a modulatory role in VLA 4 mediated cell adhesion to fibronectin . ^^^ The integrin VLA 4 ( alpha 4 beta1 ) is a receptor for fibronectin and vascular cell adhesion molecule 1 ( VCAM 1 ) . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
The results are summarized as follows : ( 1 ) cytoskeletal elements : virtually all the MMS fibroblasts were stained positively for alpha sm actin while only a small fraction of BM and of NS fibroblasts were positive for this antigen ; ( 2 ) extracellular matrix elements : MMS fibroblasts stained positively for ED B fibronectin and tenascin while the other 2 fibroblast cell types did not ; ( 3 ) cell surface molecules : NS and MMS fibroblasts expressed significantly higher levels of ICAM 1 , VLA 4 and CD 9 than BM fibroblasts . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Antibody blocking studies determined that P388D1 Mode K binding was partially dependent on beta 2 integrin ( CD 18 ) function , Mode K constitutively expressed CD 106 ( VCAM 1 ) and cell associated fibronectin , while P388D1 expressed low levels of CD49d / CD29 ( VLA 4 ) but blocking antibodies to these surface molecules did not inhibit P388D1 Mode K adherence . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Binding to a natural ECM , derived from cultured EC , or to purified fibronectin was high : 52 % + / 6 % ( n = 8 ) involved VLA 4 and VLA 5 integrins . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Interleukin 3 and Flt 3 ligand induce adhesion of Baf3 / Flt3 precursor B lymphoid cells to fibronectin via activation of VLA 4 and VLA 5 . ^^^ Whereas both Baf 3 and Baf3 / Flt3 cells expressed VLA 4 and 5 integrins as FN receptors , expression levels of VLA 4 and 5 were not affected by IL 3 or Flt 3 ligand treatment . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Immunofluorescence staining analysis for the fibronectin receptors VLA 4 and VLA 5 showed no expression on injured proximal tubules , whereas the expression of the laminin receptor VLA 6 was increased and irregular on altered PT . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Effect of the interaction between fibronectin and VLA 4 on the proliferation of human B cells , especially a novel human B cell line , OPM 3 . ^^^ However , little is known concerning the roles of the fibronectin ( FN ) / VLA 4 interaction in the proliferation of human B cells . ^^^ In this study we investigated the effect of immobilized FN on the proliferation of various B cell lines , including a newly established B cell line , OPM 3 , and human tonsillar B cells , that primarily express VLA 4 but not VLA 5 . ^^^ Immobilized FN significantly promoted the proliferation of OPM 3 cells and normal B cells via VLA 4 . ^^^ These results demonstrate that the FN / VLA 4 interaction transmits the growth signal ( s ) which may be mediated by Ras pathway in OPM 3 cells , and suggest that OPM 3 cells may be of great value in studying the roles of the FN / VLA 4 interaction in human B cell growth . . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
We demonstrate that interactions of VLA 4 with VCAM 1 , fibronectin , or a 40 kDa fragment but not a 120 kDa fragment of fibronectin supported the lateral random migration of isolated blood monocytes induced by CC chemokines , termed chemokinesis . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
CONCLUSION : Our results suggest that increased expression of VLA 4 on peripheral monocytes and augmented adhesion capacity of PBMC to fibronectin may be involved in the development of CTS in haemodialysis patients . . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Jurkat cells also adhered to VCAM 1 free sites , and binding was inhibitable by anti CS 1Fn antibody , thereby demonstrating a role for VLA 4 / fibronectin interactions especially at intraglomerular sites in acute rejection where VCAM 1 is notably absent . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Adhesion of Baf 3 cells to fibronectin was induced by IL 3 in a dose dependent manner via very late antigen 4 ( VLA 4 ; alpha4beta1 integrins ) and VLA 5 ( alpha5beta1 integrins ) activation . ^^^ Constitutively active H Ras transfected Baf 3 cells adhered to fibronectin without IL 3 stimulation through VLA 4 and VLA 5 , whereas dominant negative H Ras transfected Baf 3 cells showed significantly less adhesion induced by IL 3 compared with wild type and constitutively active H Ras transfected Baf 3 cells . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
We show here that drug sensitive 8226 human myeloma cells , demonstrated to express both VLA 4 ( alpha4beta1 ) and VLA 5 ( alpha5beta1 ) integrin fibronectin ( FN ) receptors , are relatively resistant to the apoptotic effects of doxorubicin and melphalan when pre adhered to FN and compared with cells grown in suspension . ^^^ Whether VLA 4 mediated FN adhesion offers a survival advantage over VLA 5 mediated adhesion remains to be determined . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Very late antigen 4 ( VLA 4 ) is the complex with alpha 4 and beta 1 integrins , which is the receptors to fibronectin and VCAM 1 . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Specifically , EIIIA and EIIIB containing isoforms have been implicated in the regulation of cell proliferation and migration , whereas FN isoforms containing the full length 5 region ( named V 120 ) are ligands to the VLA 4 integrin . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Integrins beta 1 or Very Late Antigens ( VLA ) are specialized transmembrane receptors allowing the attachment of the cells to collagen ( VLA 2 ) , fibronectin ( VLA 4 and 5 ) and laminin ( VLA 6 ) . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
The absence of CD 45 correlated with enhanced adhesion to fibronectin via integrin alpha5beta1 ( VLA 5 ) , but not alpha4beta1 ( VLA 4 ) in both cell lines . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
The participation of fibronectin ( FN ) and its receptor , the VLA 4 molecule , in the migration of inflammatory cells into the CNS has been proposed . ^^^ Taken together , our results suggest that FN and its receptors VLA 4 and VLA 5 might be involved in the entrance , migration and retention of inflammatory cells into the CNS during parasitic infections . . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Finally , CD 31 stimulation of eosinophils increased the adhesive function of alpha4beta1 integrin ( VLA 4 ) to its ligand fibronectin and their adhesion to IL 4 stimulated HUVECs in a VLA 4 dependent manner . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Flow cytometry indicates that cell surface expression of very late antigen 4 ( VLA 4 ) , VLA 5 , and the vitronectin receptor ( VNR ) remain constant following IFN gamma treatment , indicating the inhibitory effect of IFN gamma on adhesion to FN is not achieved through a reduction in integrin receptors for FN . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
The corresponding binding ligands to VLA 4 are vascular cell adhesion molecule 1 ( VCAM 1 ) present on the endothelial cells and the CS 1 sequence of the fibronectin present in the extracellular matrix . ^^^ The distribution of VLA 4 , VCAM 1 , CS 1 fibronectin and TGF beta was shown by immunohistochemistry using an alkaline phosphorylase method for light microscopy . ^^^ These cells often showed VLA 4 expression and co localised with areas of strong production of CS 1 fibronectin . ^^^ Normal palmar fascia contained only scanty amounts of CS 1 fibronectin , almost no VCAM 1 and only an occasional cell staining positively for VLA 4 or TGF beta . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
However , both glucose and 12 ( S ) HETE induced a 60 % increase in HAEC surface expression of connecting segment 1 ( ie , CS 1 ) fibronectin , a ligand for very late acting antigen 4 ( VLA 4 ) . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
The number of eosinophils , the level of eosinophil cationic protein ( ECP ) , the bindings of soluble intercellular adhesion molecule 1 ( sICAM 1 ) and fibronectin ( FN ) , and the expressions of CD11b ( alpha chain of Mac 1 ) and CD49d ( alpha chain of VLA 4 ) on eosinophils were evaluated in peripheral blood ( n=4 ) and BALF ( n=3 ) before and after the administration of Y 24180 . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
VCAM 1 is a primary ligand of VLA 4 , although alternatively spliced fibronectin ( FN ) containing the CS 1 region ( CS 1 FN ) also binds to VLA 4 . ^^^ CS 1 peptide and anti VCAM antibody treatment were not additive ( 50 + / 7 % inhibition ) , and 38 + / 6 % of new VLA 4 mediated adhesion to IL 1 treated HUVEC was due to an increase in CS 1 FN . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
These cells bound to fibronectin ( FN ) and to its 38 000 MW proteolytic fragment through the VLA 4 integrin that was blocked with HP2 / 1 anti alpha 4 monoclonal antibodies ( mAbs ) and with the FN peptide fragment CS 1 . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Ac cyclo ( D Lys D Ile Leu Asp Val ) ( 62 ) and beta Ala cyclo ( D Lys D Leu Leu Asp Val ) ( 68 ) were more potent than CS 1 in inhibiting the adhesion of the VLA 4 expressing MOLT 4 cells to fibronectin . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
We have previously reported that the priming of thioglycollate elicited peritoneal macrophages ( PMphi ) , as a representative population of mononuclear phagocytes ( MNP ) , by macrophage colony stimulating factor ( M CSF or CSF 1 ) rendered these cells more susceptible to secondary stimulation by extracellular matrix ( ECM ) proteins , in particular fibronectin ( FN ) , and that at least two beta 1 integrins , VLA 4 ( alpha4beta1 or CD49d ) and VLA 5 ( alpha5beta1 or CD49e ) , regulate IL 6 gene expression when PMphi come into contact with FN . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
A transduction strategy has been developed , using fibronectin ( FN ) assisted retroviral mediated gene transfer , based on the observation that hematopoietic stem and progenitor cells bind to specific adhesion domains of fibronectin , via the integrins , very late antigen 4 ( VLA 4 ) alpha 4 beta 1 and very late antigen 5 ( VLA 5 ) alpha 5 beta 1 . ^^^ Retrovirus mediated transduction on a recombinant FN fragment , FN CH 296 , containing binding sites for VLA 4 and VLA 5 , separated by type 3 repeats 12 to 14 , makes it possible to efficiently target hematopoietic stem and progenitor cells and T lymphocytes due to colocalization of target cells and retrovirus particles . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Antibodies against intercellular adhesion molecule ( ICAM ) 1 , lymphocyte function associated antigen ( LFA ) 1 , very late activation antigen ( VLA ) 1 , VLA 4 and VLA 6 , and fibronectin did not affect the potentiation of PMC histamine release by NMC reconstitution . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Further derivatisation produced compounds with IC ( 50 ) ' s < 10 nmol for inhibiting the VLA 4 mediated binding of fibronectin to RAMOS cells , providing an ideal starting point for a lead optimisation Programme . . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Consistently , vascular cell adhesion molecule 1 positive endothelial cells and a fine fibronectin network surrounding VLA 4 ( + ) mononuclear cells were found in the inflamed myocardium . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
The cecal peritoneum 24 hr after perforation was stained with specific antibodies against LFA 1 , Mac 1 , VLA 4 , ICAM 1 , VCAM 1 , and fibronectin diluted with cold University of Wisconsin ( UW ) solution in conjunction with immuno gold labeling . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
VLA 4 interacts with its ligands VCAM 1 and fibronectin ( FN ) CS 1 during chronic inflammatory diseases , such as rheumatoid arthritis , asthma , psoriasis , transplant rejection , and allergy . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
We therefore examined the efficacy of porcine very late antigen 5 ( VLA 5 ) and VLA 4 interactions with the human extracellular matrix ( ECM ) protein , fibronectin . ^^^ Human and pig PBMC adhesion to human fibronectin ( hFN ) or 120 kDa fragment coated plates was assessed following incubation with control ab , anti VLA 4 , anti VLA 5 , or soluble fibronectin . ^^^ However , porcine VLA 4 is capable of mediating adhesion to human FN . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
We report the structure activity relationships of a series of VLA 4 antagonists that were based upon the integrin binding sequence of the connecting segment peptide of fibronectin ( Leu Asp Val ) , and of VCAM 1 ( Ile Asp Ser ) , both natural ligands of VLA 4 . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Biochemical engineering of the acyl side chain of neuraminic acids activates beta ( 1 ) integrins ( VLA 4 or VLA 5 ) , resulting in an increased adhesion of HL 60 cells to fibronectin . . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Proliferative active leukocytes and those that have migrated from vessels pass through the stomata via an interaction of VLA 4 and fibronectin , adhere to the microvilli of the activated mesothelial cell surface as the result of an interaction between ICAM 1 / VCAM 1 and integrins , and exude into the peritoneal cavity . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Complete inhibition of cytokine stimulated retention was also accomplished by 4 administration of monoclonal antibodies against fibronectin or its CS 1 domain , a ligand for VLA 4 . ^^^ This increased retention was inhibited by a VLA 4 antagonist ( TBC 772 ) , a VLA 4 neutralizing antibody or by blocking one of VLA 4 ' s ligands , the CS 1 portion of fibronectin . . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Blocking antibodies to fibronectin , as well as to the integrins very late antigen 4 ( VLA 4 ) and VLA 5 , reduced monocyte adhesion to mesangial matrix by approximately 50 % . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Increased adhesions to FN and serum were abolished by a VLA 4 specific monoclonal antibody , HP2 / 1 , and a Mac 1 specific monoclonal antibody , ICRF 44 , respectively . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
In contrast , there were no remarkable differences between PB CD34+ cells before and after the 37 degrees C incubation in their expression of selectin ligand , VLA 4 , and VLA 5 or in their affinity for VCAM 1 or fibronectin . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Interaction between leukemic cell VLA 4 and stromal fibronectin is a decisive factor for minimal residual disease of acute myelogenous leukemia . ^^^ We found that VLA 4 positive cells acquired resistance to anoikis ( loss of anchorage ) or drug induced apoptosis through the phosphatidylinositol 3 kinase ( PI 3K ) / AKT / Bcl 2 signaling pathway , which is activated by the interaction of VLA 4 and fibronectin . ^^^ Thus , the interaction between VLA 4 on leukemic cells and fibronectin on stromal cells may be crucial in bone marrow MRD and AML prognosis . . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Concomitantly , membrane expression of fibronectin and laminin receptors ( VLA 4 , VLA 5 and VLA 6 ) was also enhanced on thymocyte subsets of infected mice . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
IDV appeared to act upon VLA 4 and VLA 5 since addition of antagonist monoclonal antibodies ( mAb ) similarly reduced adhesion of non treated DC to fibronectin . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
We first demonstrated that adhesion to Fn was stimulated in S / G2 + M as compared to G0 / G1 , in ex vivo cultured CD34+ cells , with a predominant usage of very late antigen ( VLA ) 5 over that of VLA 4 . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
CD 81 facilitates both VLA 4 mediated leukocyte rolling and arrest on VCAM 1 under shear flow as well as VLA 5 dependent adhesion to fibronectin during short stationary contacts . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Here we show that the BCR controls integrin alpha4beta1 ( VLA 4 ) mediated adhesion of B cells to vascular cell adhesion molecule 1 and fibronectin . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
HMR 1031 is a potent and specific antagonist of the integrin VLA 4 ( alpha4beta1 ) binding to vascular cell adhesion molecule 1 ( VCAM 1 ) and fibronectin . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
The alpha4beta1 integrin ( VLA 4 ) is a leucocyte ligand for endothelial vascular cell adhesion molecule 1 ( VCAM 1 ) , fibronectin and osteopontin . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Coincubation of eosinophils with integrin blocking monoclonal antibodies in adhesion assays showed that an association of the VLA 4 , LFA 1 , and Mac 1 integrins mediate the adhesion of SCA eosinophils to FN . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
We first showed , by immunocytochemistry , cytofluorometry , and RT PCR , that PTR produce ECM components , including fibronectin and laminin , and express the corresponding integrin type receptors , VLA 4 , VLA 5 , and VLA 6 . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Both ALA and DHLA downmodulated the surface expression of the alpha4beta1 integrin ( very late activation 4 antigen ; VLA 4 ) , which binds fibronectin and its endothelial cell ligand vascular cell adhesion molecule 1 ( VCAM 1 ) . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Also , PRL promotes the adhesion of PBMC to immobilized intercellular adhesion molecule 1 and fibronectin , ligands for LFA 1 and VLA 4 , respectively . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
The beta 1 integrin very late antigen 4 ( VLA 4 ) plays a key role in lymphocyte rolling and adhesion to endothelium , and in lymphocyte migration through fibronectin . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Proliferation of uncultured LTC IC was inhibited by VLA 4 mediated binding to VCAM 1 and the CS 1 domain of Fn , as well as binding to P selectin . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Neutralisation of TGF beta or binding of VLA 4 to fibronectin prevents rat tendon adhesion following transection . ^^^ Experimentally this has been achieved by neutralisation of transforming growth factor beta over the first 3 days following injury , or by blockade of inflammatory cell binding to the CS 1 locus on fibronectin with an anti VLA 4 antibody , or with the synthetic VLA 4 inhibitor , CS 1 peptide , in a rat model of tendon transection . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Cell to cell contact is required to support progenitor activity , but can be replaced if receptor ligand engagement of the VLA 4 and LFA 1 complexes is provided through the use of recombinant ligands ( fibronectin , ICAM 1 , VCAM 1 ) . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
We also show that VEGFR1+ cells express VLA 4 ( also known as integrin alpha4beta1 ) , and that tumour specific growth factors upregulate fibronectin a VLA 4 ligand in resident fibroblasts , providing a permissive niche for incoming tumour cells . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Human hematopoietic progenitor cells express very late antigen 4 ( VLA 4 ) and VLA 5 , which mediate their interaction with fibronectin by recognizing the connecting segment 1 ( CS 1 and RGD motifs , respectively . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Here we show that integrin alpha4beta1 ( VLA 4 ) promotes the homing of circulating progenitor cells to the alpha4beta1 ligands VCAM and cellular fibronectin , which are expressed on actively remodeling neovasculature . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Basement membrane proteins , laminin , and collagen type 4 , as well as vitronectin and fibronectin , were identified in the intima and there was corresponding expression of integrin and non integrin receptors ( e . g . , CD 29 , CD49b , CD49d , CD49e , CD49f , CD 51 , CD 61 , CD 44 ) for these matrix proteins . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
The alpha 4 beta 1 integrin ( CD49d , CD 29 ) constitutively expressed on leukocytes regulates cell migration to inflammatory sites , cell activation , and development through its interactions with two alternate ligands , vascular cell adhesion molecule 1 ( VCAM 1 ; CD 106 ) expressed on cytokine activated endothelium , dendritic and stromal cells , and the extracellular matrix protein fibronectin . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Analysis of the expression of adhesion receptors on the canine peripheral lymph DC revealed the presence of CD 54 , CD 58 , CD 18 as well as CD49d and CD49e molecules and cell surface fibronectin . ^^^ Antibody against fibronectin , a substrate for CD49d and CD49e receptors , reduced DC lymphocyte binding . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
In vitro adhesion of lymphocytes to fibronectin , being the extracellular matrix ligand of CD49d , was also investigated . ^^^ Furthermore , CD49d ( low ) B CLL cells had lower adhesion to fibronectin , whereas CD49d ( high ) B CLL cells showed normal adhesion ratios ( P < 0 . 002 ) . ^^^ On the basis of these results , we concluded that early stages of CLL are correlated with the expression of CD49d ( low ) , CD11a ( low ) , CD11b ( high ) , CD 13 , CD 33 , sIgM ( low ) and also had lower fibronectin adhesion , whereas advanced stages of CLL are associated with CD49d ( high ) , CD11a ( high ) , CD11b ( low ) , CD13+ , CD33+ , SIgM ( high ) and show normal fibronectin adhesion . . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Significant differences in the expression of very late antigen ( VLA ) integrins , VLA alpha 4 chain ( CD49d ) , the classical fibronectin receptor VLA alpha 5 chain ( CD49e ) , leukocyte function associated molecule 3 ( LFA 3 ; CD 58 ) and fibronectin were observed between patients showing good ( > 10 % ) or poor ( < 10 % ) penetration in the sperm penetration assay ( P = 0 . 0068 ) . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Spermatozoa were incubated with antibodies against alpha 1 ( CD49a ) , alpha 2 ( CD49b ) , alpha 3 ( CD49c ) , alpha 4 ( CD49d ) , alpha 5 ( CD49e ) , alpha 6 ( CD49f ) chains of beta 1 integrins , beta 1 ( CD 29 ) , beta 2 ( CD 18 ) , alpha 5 ( CD 51 ) , beta 3 ( CD 61 ) and beta 4 integrin chains , the LFA 3 ( Lymphocyte function antigen , CD 58 ) from the immunoglobulin superfamily and the extra cellular matrix proteins laminin , fibronectin and collagen 4 . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
We also demonstrated that increased expression of CD49d on T cell lines 24 h and 48 h after stimulation correlated with increased adhesion to the CS 1 fragment of fibronectin . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
We have observed that in patients with high expression of laminin receptors CD49b , CD49f and lack of fibronectin receptors CD49d , CD49e more often renal failure has been confirmed . . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Regarding other adhesion molecules on EoL 1 ( CD11a , CD 18 , CD49d ) , the expression was not significantly augmented by adherence to FN . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
CD49d integrin expression conferred mature MDDC with an elevated capacity to adhere to the CS 1 fragment of fibronectin , and also mediated transendothelial migration of mature MDDC . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Furthermore , mAb ' s specific for CD49d and CD 106 , or the synthetic CS 1 fibronectin peptide , could inhibit B cell pseudoemperipolesis . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Studies using blocking antibodies indicated the involvement of CD11a in binding to ICAMs , and of CD49d , CD49e , and CD 29 in binding to FN . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Therefore , we analyzed the expression of the FN receptors , VLA 4 ( CD49d ) and VLA 5 ( CD49e ) , by direct immunofluorescence , on these leukemic cells . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Adhesion to fibronectin via alpha 4 integrin ( CD49d ) protects B cells from apoptosis induced by serum deprivation but not via IgM or Fas / Apo 1 receptors . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
METHODS : Samples were taken at 2 , 10 , and 48 hours for histological and immunohistochemical studies using monoclonal antibodies against human CS 1 fibronectin , CCL 17 , CD 3 , CD 68 , CD49d , CXCR 3 , CCR 5 , and CCR 3 . ^^^ RESULTS : At positive antigen stimulated sites there was an early expression of CS 1 fibronectin ( 2 hours ) , followed by CCL 17 and a later accumulation of alplha4 / beta1+ ( CD49d ) , CD3+ , CD68+ , CXCR3+ and CCR5+ mononuclear cells . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Adhesion binding experiments showed that CD106+ activated CD29+ BM PC from these patients adhered to fibronectin ( FN ) in a CD29 / CD49d dependent manner . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
The expressions of CD49d and CD 44 and the adhesive ability of mononuclear cells to bone marrow matrix ( fibronectin ) were examined by flow cytometry and ( 51 ) Cr adhesive assay , respectively . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
RESULTS : ( 1 ) The expression of CD49d , CD 44 , CD11a and CD49e on expanded CD 34 ( + ) cells increased or sustained the same levels as those of the fresh isolated UCB CD 34 ( + ) cells , while the expression of CD62L , CD 54 and CD 31 on expanded CD 34 ( + ) cells declined during the culture . ( 2 ) The spontaneous adhesion between CD 34 ( + ) and FN and SDF 1 induced adhesion continuously increased in the course of the first 10 day culture . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
Monocytes of type 1 diabetic patients displayed an increased adhesion to fibronectin in comparison with type 2 patients and healthy control subjects but had a normal expression of the FN binding integrins CD 29 , CD49a , CD49d , and CD49e ( although CD11b and CD 18 expression was increased ) . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
MGDF and FN increased expression of tetraspanin molecules , CD 63 and CD 151 , as well as their co expression with the beta ( 1 ) integrins on both the CD 34 ( + ) and CD 34 ( ) cells ( e . g . and increase from 0 % to 80 % co expression of CD49d and CD 151 on CD 34 ( + ) ) . ^^^ DISCUSSION : FN enhanced Mk progenitor generation through modulation of beta ( 1 ) integrin tetraspanin complexes , such as CD151 / CD49d . . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
When compared to macrophages from control strains , resident NOD macrophages showed a reduced ability to adhere to and migrate on FN , a delayed clearance following peritoneal inflammation , and substantially lower expression levels of the alpha4beta1 integrin alpha chain , CD49d . ^^^ Mature NOD macrophages are characterized by decreased CD49d expression and show defective CD49d mediated adhesion to FN . . ^^^
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P13612 and P02751	Pubmed	SVM Score :0.0
NA