| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| BM 180 has a mobility of 180 and 60 kDa in the absence or presence of dithiothreitol , respectively , and shows no immunological identity by competitive enzyme linked immunosorbent assay with laminin 1 , collagen 4 , entactin , fibronectin , BM 40 , perlecan , or vitronectin . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| More moderate affinities were observed for fibulin 1 , laminin 1 and perlecan , while several other ligands such as collagens , nidogen 1 , fibronectin and BM 40 showed little or no binding . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Other extracellular matrix molecules , such as BM 40 ( also known as SPARC / osteonectin ) , thrombospondins 1 and 2 , fibronectin , nidogens 1 and 2 , and collagen types 8 , 15 , and 18 , are also differentially expressed by endothelium , varying with the endothelium type and / or pathophysiological state . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| In bone , the ECM is composed of proteins such as collagen ( CO ) , fibronectin ( FN ) , laminin ( LM ) , vitronectin ( VN ) , osteopontin ( OP ) and osteonectin ( ON ) . ^^^ ECM components , including CO , FN , LM , ON , OP and VN , produced on the surface of the polymers were quantified by ELISA and localized by immunofluorescence staining . ^^^ On PLAGA , OP and ON were the most abundant ECM components , followed by CO , FN , VN and LN . ^^^ Immunofluorescence revealed an extracellular distribution for CO and FN , whereas OP and ON were found both intracellularly as well as extracellularly on the polymer . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| The effects of the extracts on cell growth was measured using the MTT assay and an ELISA assay was used to measure the expression of bone sialoprotein ( BSP ) , osteonectin ( ON ) and fibronectin ( FN ) , antigens which play a fundamental part in the integrity and function of hard connective tissue . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Compared with CCs of 1 week , PDLs and ABCs after 2 weeks revealed relatively high levels of all analyzed mRNAs , viz . , for EGF , HGF , VEGF , TGFbeta 1 , collagen 1 ( COL 1 ) , osteonectin ( ON ) , fibronectin ( FN 1 ) , and MMP 13 . ^^^ Analysis of our data indicated that the expression of mRNAs for growth factors and for COL 1 , ON , FN 1 , and MMP 13 was modulated in the distinct cell types with respect to culture time and culture type . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Expression of major adhesive ECM molecules , constituting the endothelial basement membrane ( i . e . , fibronectin , laminin A , B and collagen 4 ) and of other attachment factors , such as SPARC ( osteonectin ) , tenascin and thrombospondin 1 , was examined . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| In addition to the production of type 1 collagen , these cells also synthesized fibronectin and osteonectin . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| The known genes included alpha 1 ( 1 ) procollagen , alpha 2 ( 1 ) procollagen , fibronectin , ferritin heavy chain , insulinlike growth factor binding protein 3 ( IGFBP 3 ) , osteonectin , human tissue plasminogen activator inhibitor type 1 , thrombospondin , and alpha B crystallin . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| That these antilaminin antibodies were , at least in part , responsible for the toxicity was indicated 1 ) by reduced cultured embryo toxicity for six of seven serum samples after pre absorption with purified laminin , 2 ) by demonstrating a relationship between the amount of affinity purified antilaminin IgG added to control serum for culture and the severity of embryo abnormalities seen at the end of culture , and 3 ) by the sera ' s failing to react with other basement membrane proteins , including type 4 collagen , fibronectin , osteonectin , and heparan sulfate proteoglycan . . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| The present study focused on the localization and distribution of the major macromolecules that are normally encountered in cartilage and bone , including collagens , proteoglycans , fibronectin , osteonectin , osteocalcin , alkaline phosphatase , and anchorin CII . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| NE secreted soluble laminin , osteonectin , entactin and fibronectin but did not form visible pericellular matrix . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Restriction fragment length polymorphisms for growth hormone , prolactin , osteonectin , alpha crystallin , gamma crystallin , fibronectin and 21 steroid hydroxylase in cattle . ^^^ Eleven RFLPs were identified using seven different probes : growth hormone , prolactin , osteonectin , alpha A crystallin , gamma crystallin , fibronectin and 21 steroid hydroxylase . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Utilizing immunohistochemical techniques , positive detection of ECM proteins within the ( pre ) odontoblast or in their extracellular matrices has been demonstrated for ( pro ) collagen type 1 , 3 , 4 , 5 and 6 , fibronectin , tenascin , laminin , basement membrane heparan sulfate , nidogen , dentinophosphophoryns ( DPP ) , osteocalcin ( OC ) , osteonectin , osteopontin and 95 kDal glycoprotein . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Competitive binding studies showed that bovine serum albumin , fibronectin , and bovine bone 34K glycoprotein ( osteonectin ) did not compete with phosphophoryn and did not inhibit its binding to collagen fibrils . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Several other proteins found in bone , namely fibronectin , type 1 collagen , and osteonectin , were not chemotactic . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| None of the proteins known to interact with thrombospondin ( fibrinogen , fibronectin , collagen , plasminogen ) had a significant inhibitory effect on thrombospondin osteonectin complex formation . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| The mRNAs for type 1 procollagen and osteonectin were also increased by TGF beta 1 but fibronectin mRNA was decreased . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Recent advances in the isolation and characterization of a large number of different extracellular matrix macromolecules ( eg various types of collagens , fibronectin , chondronectin , osteonectin , laminin , proteoglycans , enamel protein ) now suggest a renewed interest in this problem area , and also the promise for significant breakthroughs in the near future . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Not only have specific bone cell matrix components ( collagen , osteonectin , the large chondroitin sulfate proteoglycan , biglycan , and decorin ) been found to be present in reduced levels in OI bone cells , but some matrix components ( thrombospondin , fibronectin , and hyaluronan ) have also been found to be present in elevated levels in the matrix of OI cells . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Histological aspects and the transcriptional phenotypes ( transcripts of TGF beta s , BMPs , msxs , IGF 1 , fibronectin , osteonectin , bone sialoprotein genes ) are very similar in vivo and in vitro . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Comparative analysis of TGF beta s , BMPs , IGF 1 , msxs , fibronectin , osteonectin and bone sialoprotein gene expression during normal and in vitro induced odontoblast differentiation . ^^^ Normal odontoblast and in vitro induced odontoblast like cells expressed transcripts encoding for TGF beta 1 and 3 , BMP 2 and 4 , bone sialoprotein and osteonectin whereas either ubiquitous expression or no expression could be detected for TGF beta 2 , IGF 1 or fibronectin mRNAs . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| These appear to be useful inflammatory markers and may be distinguished from products of connective tissue breakdown which include collagenous and non collagenous products , including collagen peptides , osteonectin , and fibronectin . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Osteonectin / SPARC regulates cellular secretion rates of fibronectin and laminin extracellular matrix proteins . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Application of ICF over this time did not alter the synthesis of type 1 collagen , fibronectin or bone SPARC ( osteonectin ) compared to that of control cells . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Serial sections were processed by indirect immunogold cytochemistry using primary antibodies against human fibronectin , vitronectin , bone sialoprotein , alkaline phosphatase , osteonectin , and osteopontin . ^^^ Large amounts of osteonectin and fibronectin , together with vitronectin , were localized on the microfilament aggregates , which were often associated with altered elastic fibers in PXE dermis and were never visualized in the dermis of control subjects . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Osteopenia due to deficient extracellular matrix synthesis is a hallmark of osteogenesis imperfecta ( OI ) , Previous studies carried out within 72 h of osteoblast subculture , at an early stage of matrix synthesis , indicated that for osteoblasts derived from human OI patients the total amounts of collagen , osteonectin , and three proteoglycans were significantly reduced , while total amounts of thrombospondin , fibronectin , and matrix hyaluronan were elevated compared with age matched controls . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Osteocytes ( and osteoblasts ) adhere to the same substrates ( i . e . , collagen types 1 and 2 , collagen fibers , osteopontin , osteonectin , fibronectin , fibrinogen , thrombospondin , and laminin ) . ^^^ Cell spreading varied between substrates , from all cells rounded on thrombospondin to all cells fully spread out on osteopontin , osteonectin , vitronectin , fibronectin , fibrinogen , and laminin . ^^^ The adhesion of osteocytes and osteoblasts to osteopontin , osteonectin , vitronectin , and fibrinogen was strongly inhibited , and to fibronectin and laminin moderately , by an RGD peptide . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| During the bone matrix formation / maturation period ( days 10 16 ) , type 1 collagen expression and biosynthesis , fibronectin , TGF beta 1 and osteonectin expressions were high and maximal around day 16 . ^^^ The other is the expression pattern of fibronectin and osteonectin , which was higher in the maturation and mineralization periods than in the proliferation period . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| With an immunocytochemical method we determined the presence of collagen type 1 , fibronectin , osteocalcin , osteopontin and osteonectin in cultures of isolated chicken osteocytes , osteoblasts and periosteal fibroblasts . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Matrix proteins thought to be crosslinked by transglutaminases in cartilage and bone matrix include glycoproteins such as osteonectin , osteopontin , fibronectin , fibrillin , and collagens 2 , 3 , 5 , and 11 . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Their platelet alpha granular proteins factor 5 , thrombospondin , von Willebrand factor , fibrinogen , fibronectin , osteonectin , and P selectin were proteolyzed and comigrated with the degradation products found in patients from the other family . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| In this study the presence of the ECM / BM components fibronectin , collagen 4 , alpha 1 , beta 1 , gamma 1 laminin , several laminin variants , osteonectin , and perlecan was demonstrated in cryosections of nonadenomatous and toxic adenoma human thyroid tissue . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| After birth , osteonectin and fibronectin were mainly associated with blood vessels . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| All three HERKO lines expressed high amounts of collagen 1A1 , osteopontin , osteonectin , fibronectin , decorin , biglycan , and alkaline phosphatase . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Construction of cDNA libraries from two conditional cell lines and application of differential screening and subtractive hybridization techniques have resulted in the cloning of eight senescence induced genes ( SGP 2 / Apo J , alpha 1 procollagen , osteonectin , fibronectin , SM 22 , cytochrome C oxidase , GTP alpha , and a novel gene ) and a senescence repressed gene ( FRS 2 ) . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| The patients with VWD had partial to complete deficiencies of plasma and platelet VWF but normal alpha granular multimerin and factor 5 , and normal alpha granular fibrinogen , thrombospondin 1 , fibronectin , osteonectin and P selectin . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Spatial and temporal expression of genes for major collagens ( Types 1 and 2 ) , minor fibrillar collagens ( Types 4 and 11 ) , and several extracellular matrix components ( osteocalcin , osteonectin , osteopontin , fibronectin and CD 44 ) are detected by in situ hybridization . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| In addition , the histochemical expression of the bone matrix proteins osteonectin , osteopontin and fibronectin was compared to that of E 11 using monoclonal antibodies . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| The in situ expression of amelogenins , keratins , collagens type 3 and 4 , vimentin , fibronectin , osteonectin , and osteocalcin was performed on normal and tumor odontogenic human cells . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Moreover , immunohistochemical staining in osteoblasts with zirconia and alumina showed the capacity of these cells to elaborate the extracellular matrix composed of types 1 and 5 collagen , osteocalcin , osteonectin , bone sialoprotein , and cellular fibronectin . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Flow cytometry analysis was carried out using rabbit antibodies against osteonectin , osteopontin , bone sialoprotein , fibronectin , and collagen type 1 to measure the effects of the materials on key cellular functions . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| The extent of accumulation of fibrillar collagen and of fibronectin differed , while those of other proteins in the ECM , such as tenascin , osteocalcin and osteonectin , were similar at the two sites . . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| These corresponding genes include fibronectin , osteonectin , alpha 1 ( 1 ) procollagen , apolipoprotein J , SM 22 , SS 9 , and GTP alpha binding protein . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| We found that the genes human TGF beta induced gene product ( betaig h 3 ) , osteoblast specific factor 2 , osteonectin , fibronectin , and type 1 collagen were all downregulated in the affected skin fibroblasts , with betaig h 3 the most significantly affected . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Thus , antibodies raised against extracellular matrix proteins , laminin , fibronectin , entactin or nidogen , tenascin , heparan sulfate proteoglycan , osteonectin , and Type 4 collagen reacted positively with basal lamina proteins . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Proliferation was highest in primary rat calvarial osteoblasts cells grown on fibronectin but less on type 1 collagen ; osteonectin and poly L lysine did not support early proliferation . ^^^ Fibronectin and type 1 collagen binding requires integrins , whereas cell adhesion to osteonectin or poly L lysine does not involve integrins . ^^^ Therefore , the role of integrins in osteoblast signaling , leading to the induction of AP 1 transcription factors ( c fos and c jun ) which are important in cell proliferation , was studied . c fos and c jun message levels were increased at 60 min in osteoblasts plated onto fibronectin or collagen , but not in cells on osteonectin or poly L lysine . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| In this work , we show that transforming growth factor beta 1 ( TGF beta 1 ) regulates the induction of several of these biomarkers in SIPS : cellular morphology , senescence associated beta galactosidase activity , increase in the steady state level of fibronectin , apolipoprotein J , osteonectin , and SM 22 mRNA . ^^^ In the presence of each of these antibodies , the steady state level of fibronectin , osteonectin , apolipoprotein J , and SM 22 mRNA is no more increased at 72 h after stress . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| The enzyme could also degrade osteonectin , vitronectin , and fibronectin , but not laminin and albumin . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Stenotic ligamentum flavum ( SLF ) cells were seen to express high levels of alkaline phosphatase ( ALP ) activity and to produce a matrix rich in type 1 and 3 collagen , fibronectin and osteonectin . ^^^ Cultures from control patients showed neither osteoblastic nor chondrocytic features : they expressed type 1 and type 3 collagen and fibronectin , but did not stain for osteonectin , nor were bone like calcifications observed in presence or absence of betaGP . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Cultured cells were immunoreactive for type 1 and type 3 collagen , osteonectin , and fibronectin ; when ascorbic acid and beta glycerophosphate were added , they synthesized a rich extracellular matrix . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Moreover , the transduced cells grown at 34 . 5 degrees C were also found to express bone sialoprotein , osteopontin and type 1 collagen at levels similar to those of the parental cells at 37 degrees C , although osteonectin and fibronectin were down regulated . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Expression of osteonectin was also associated with reduced adhesion to vitronectin , but not to fibronectin . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| In this study . human osteosarcoma MG 63 were used in short term in vitro tests to assay for cell viability and cell proliferation at 12 , 24 and 72 h while SaOS 2 were used in long term in vitro tests to assay for osteonectin , osteopontin , osteocalcin gene expression , total protein amount ( TP ) . alkaline phosphatase activity ( ALP ) and fibronectin production ( FN ) for 1 4 weeks . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Decrease in osteonectin , fibronectin , and collagen types 6 and 16 mRNAs was observed . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Third , the steady state level of the mRNA of three senescence associated genes , i . e . fibronectin , osteonectin and SM 22 , was increased in HDFs at 72 h after three and five exposures to UVB . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Several cell growth and differentiation related genes , including thrombospondin 1 ( THBS 1 ) , SPARC [ secreted protein , acidic , cysteine rich ( osteonectin ) ] , and fibronectin ( FN 1 ) were also found to be upregulated in the nontumorigenic clones ; however , these were expressed in the slow growing tumorigenic clones as well . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Expressions of fibronectin and osteonectin were assessed through immunogold labelling by scanning electron microscopy . ^^^ Further , osteoblastic cells on the grooved surfaces also displayed a strong labelling for fibronectin at the cytoplasmic extensions coupled with intense osteonectin expression in comparison to the rough surfaced implants . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| A striking finding of the ultrastructural immunocytochemical investigations was the synthesis and deposition of bone related proteins ( osteonectin , fibronectin , fibronectin receptor ) by osteoblasts from day one of bone / biomaterial interaction . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Tenascin ( TN ) , fibronectin ( FN ) , type 1 ( col 1 ) and 3 collagen ( col 3 ) and osteonectin ( ONEC ) were detected by immunofluorescence . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| We have found that the presence of low levels of adhesion proteins , e . g . fibronectin , together with high concentrations of non adhesion proteins , e . g . osteonectin , can promote cell attachment on plastic culture dishes . ^^^ Adding the adhesion protein fibronectin to the plate first , followed by osteonectin , resulted in no ' activation ' of attachment . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| This new formed tissue is markedly positive for several antibodies for bone , including osteonectin , bone sialoprotein , osteocalcin , fibronectin , collagen 3 , and bone alkaline phosphatase ( BALP ) . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| For gene analysis , conventional and quantitative polymerase chain reactions were used for COL1A2 , COL2A1 , aggrecan , BMP 2 , biglycan , decorin , osteonectin , fibromodulin , fibronectin , matrix metalloproteinase 13 ( MMP 13 ) , and tissue inhibitor of MMP 1 . ^^^ Gene expression analysis showed a significant up regulation of COL1A2 , osteonectin , decorin , fibronectin , tissue inhibitor of MMP 1 , BMP 2 , and MMP 13 in compressed discs . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| To evaluate extracellular matrix genes , quantitative real time reverse transcriptase polymerase chain reaction was applied for collagen 1 , collagen 2 , biglycan , decorin , fibromodulin , fibronectin , aggrecan , and osteonectin . ^^^ Matrix gene expression was characterized by two major developments : collagen 1 and 2 , biglycan , and decorin showed early elevation followed by later downregulation to control levels , whereas fibromodulin , fibronectin , aggrecan , and osteonectin showed continuous upregulation or remained at similar levels . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| In this study , we investigated the effects of several growth factors and cytokines on the synthesis of DNA , alkaline phosphatase ( ALPase ) , fibronectin , and secreted protein acidic and rich in cysteine ( SPARC ) in human periodontal ligament ( HPL ) cells . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| We now show that addition of purified SPARC protein , or an N terminal synthetic peptide ( SPARC 4 23 ) , to strains of bovine aortic endothelial cells undergoing angiogenesis in vitro resulted in a dose dependent decrease in the synthesis of fibronectin and thrombospondin 1 and an increase in the synthesis of type 1 plasminogen activator inhibitor . ^^^ SPARC decreased fibronectin mRNA by 75 % over 48 h , an effect that was inhibited by anti SPARC immunoglobulins . ^^^ Half maximal induction of PAI 1 mRNA or inhibition of fibronectin and thrombospondin mRNAs occurred with 2 5 micrograms / ml SPARC and approximately 0 . 05 mM SPARC 4 23 . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| TGF beta promotes tissue matrix formation by stimulating both the synthesis of matrix proteins ( collagen , fibronectin and SPARC ) and proteinase inhibitors ( TIMP , PAI 1 ) and by decreasing the synthesis of MMPs , but not the 72 kDa gelatinase . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Moreover , levels of mRNA for fibronectin and SPARC ( Secreted Protein that is Acidic and Rich in Cysteine ) in network forming cells were similar to levels seen in endothelial cells that did not form networks . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| However , DNA synthesis was not affected by SPARC when the cells were plated on gelatin or fibronectin in serum free medium . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Transfected fetal calf ligament fibroblasts showed significantly reduced levels of endogenously produced type 1 collagen , as determined by imaging and digital quantitation of immunofluorescence by confocal microscopy ; synthesis of fibronectin , thrombospondin , and SPARC ( secreted protein , acidic and rich in cysteine ) was unchanged or increased in these cells . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Decreased amounts of collagen and fibronectin , but not of SPARC ( secreted protein , acidic and rich in cysteine ) in the conditioned medium was the result of MMP activity since steady state mRNA levels and transcription of the respective matrix protein genes were unaffected by ConA . . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Immunohistochemical localization of type 1 collagen ( CI ) , fibronectin ( FN ) , secreted protein , acidic and rich in cysteine ( SPARC ) , vitronectin ( VN ) , and bone sialoprotein ( BSP ) was performed on 6 microns thick sections . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| We used an in vitro model of rat lung branching morphogenesis to examine airway branching in the presence of : a ) a neutralizing anti SPARC antibody ; or b ) a synthetic peptide from a region of SPARC that , like the native protein , perturbs cell adhesion and diminishes the synthesis of fibronectin and thrombospondin 1 . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Following treatment with endotoxin , endothelial cell monolayers altered their production of [ 3H ] proline labeled procollagen ( types 3 and 5 ) , fibronectin , and a 43 kDa protein , Secreted Protein Acidic and Rich in Cysteines ( SPARC ) . ^^^ Our results show that endotoxin decreased the levels of procollagen and fibronectin , whereas the production of SPARC was augmented approximately twofold . ^^^ Northern blot analyses revealed no changes in the expression of SPARC or beta actin transcripts , whereas the expression of fibronectin transcript increased two to threefold . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Analysis of radiolabeled proteins in the secreted fraction by sodium dodecyl sulfate ( SDS ) polyacrylamide gel electrophoresis ( PAGE ) revealed prominent protein bands at 220 and 49 . 5 kD in both diabetic and nondiabetic cultures that were identified by immunoblot analysis as fibronectin and a mixture of secreted protein acidic and rich in cysteine ( SPARC ) and plasminogen activator inhibitor 1 ( PAI 1 ) , respectively . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| In general , however , the level of NaB induced SPARC expression was considerably reduced in FN cultures correlating with a lower efficiency of CE formation . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Collagenase was induced within 4 h in cells treated with phorbol diesters or plated on fibronectin fragments , but was induced after 8 h in cells treated with SPARC . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Among them are six known sequences coding for : acid sphingomyelinase , fibronectin , SPARC , nm 23 metastasis suppressor protein , and two translation factors , eIF 2 beta and EF 1 alpha . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Secreted extracellular proteins included fibronectin , collagen types 1 and 4 , laminin , and SPARC ( secreted protein , acidic , and rich in cysteine ) . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Several matricellular proteins , proteoglycans , and growth factors bind to type 1 collagen , e . g . , fibronectin , secreted protein acidic and rich in cysteine ( SPARC ) , decorin , and transforming growth factor beta . ^^^ However , accumulation of extracellular SPARC was severely affected in the tissues of Mov 13 homozygotes , whereas extracellular deposition of the secreted glycoproteins fibronectin and type 3 collagen was not altered . ^^^ |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Differential effects of various growth factors and cytokines on the syntheses of DNA , type 1 collagen , laminin , fibronectin , osteonectin / secreted protein , acidic and rich in cysteine ( SPARC ) , and alkaline phosphatase by human pulp cells in culture . ^^^ Adding transforming growth factor beta ( TGF beta ) decreased laminin and ALPase levels , whereas it increased SPARC and fibronectin levels 3 to 10 fold . ^^^ Basic fibroblast growth factor ( bFGF ) decreased type 1 collagen , laminin , SPARC , and ALPase levels without changing the fibronectin level . ^^^ These findings demonstrated that TGF beta , bFGF , EGF , PDGF , TNF alpha , and IL 1beta have characteristically different patterns of actions on DNA , laminin , type 1 collagen , fibronectin , ALPase , and SPARC syntheses by pulp cells . . ^^^ |
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| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Several extracellular matrix proteins including fibronectin , thrombospondin 1 , laminin , SPARC , and osteopontin have been suggested to modulate tumor phenotype by affecting cell migration , survival , or angiogenesis . ^^^ |
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| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Expression of various proteins , including SPARC ( secreted protein acidic and rich in cysteines ) , type 4 procollagen and fibronectin ( FN ) was examined by radiolabeling the cells with [ 3H ] proline , immunoprecipitation with specific antibodies , and Northern blot analyses by using specific cDNA probes . ^^^ |
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| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| To determine whether SPARC alters the growth , attachment , or migration of gliomas , we have used U87T2 and doxycycline regulatable SPARC transfected clones to examine the effects of SPARC on ( 1 ) cell growth , ( 2 ) cell cycle progression , ( 3 ) cell attachment , and ( 4 ) cell migration , using growth curves , flow cytometry , attachment , and migration analyses on different brain ECMs , including collagen 4 , laminin , fibronectin , vitronectin , hyaluronic acid , and tenascin . ^^^ In comparison to the parental U87T2 clone , the SPARC transfected clones demonstrated increased attachment to collagen , laminin , hyaluronic acid , and tenascin , but not to vitronectin or fibronectin . ^^^ |
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| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Effects of transforming growth factor beta 1 and fibronectin on SPARC expression in cultures of human periodontal ligament cells . ^^^ Transforming growth factor beta ( 1 ) ( TGF beta ( 1 ) ) increases synthesis of secreted protein , acidic and rich in cysteine ( SPARC ) , as well as fibronectin ( FN ) and type 1 collagen . ^^^ We examined the effect of FN on SPARC expression by TGF beta ( 1 ) in cultures of human periodontal ligament cells ( HPL cells ) . ^^^ HPL cells cultured on FN coated plates secreted more SPARC than those on non coated plates . ^^^ This study demonstrated that FN may be partly involved in the increase in SPARC expression by TGF beta ( 1 ) in HPL cells . . ^^^ |
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| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Expression and regulation of SPARC , fibronectin , and collagen 4 by dexamethasone in lens epithelial cells . ^^^ We have examined the effects of dexamethasone ( DEX ) on the regulation of Secreted Protein Acidic and Rich in Cysteines ( SPARC ) , fibronectin ( FN ) , and collagen 4 ( CN 4 ) . ^^^ Thus showing a differential upregulation by DEX of mRNAs of SPARC , FN , and CN 4 . ^^^ |
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| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Direct comparison of EPC samples with the MM samples identified 156 genes significantly upregulated and 85 genes significantly downregulated ( P < 0 . 005 , t test ) in the EPCs , including several genes involved in angiogenesis and adhesion that were upregulated ( including angiopoietin 1 , SPARC , Notch 3 and fibronectin 1 ) . ^^^ |
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| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Cells were plated on poly l lysine or fibronectin and treated with tenascin , Hep 1 , or SPARC peptides to inhibit focal adhesion formation . ^^^ |
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| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Moreover , genes associated with invasion or proliferation were upregulated , including genes such as fibronectin , secreted protein acidic and rich in cystein ( SPARC ) , cathepsin B and KRT 17 . ^^^ |
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| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| The associated ECM is rich in collagen 3 , SPARC , and new blood vessels ( CD 31 ) but is depleted of collagen 1 and fibronectin . ^^^ |
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| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Interestingly , cardiac myocytes plated on FN showed elevated expression of the fibrosis promoting peptides connective tissue growth factor ( CTGF ) , WNT 1 inducible signaling pathway protein 2 ( WISP 2 ) , and secreted acidic cysteine rich glycoprotein ( SPARC ) . ^^^ |
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| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| Herein , we report that SPARC expression does not significantly affect fibronectin induced cell spreading but enhances fibronectin induced stress fiber formation and cell mediated partial unfolding of fibronectin molecules , an essential process in fibronectin matrix assembly . ^^^ Cells lacking SPARC exhibit diminished fibronectin induced integrin linked kinase activation and integrin linked kinase dependent cell contractile signaling . ^^^ Furthermore , induced expression of SPARC in SPARC null fibroblasts restores fibronectin induced integrin linked kinase activation , downstream signaling , and fibronectin unfolding . ^^^ |
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| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09486 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|