Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P08648 and P02751	Pubmed	SVM Score :1.1270015
Inhibition of interactions between FN and integrin alpha 5 beta 1 by the competitive GRGDSP peptide or anti alpha 5 integrin antibody restricted cell spreading , reduced cell surface staining for alpha 5 beta 1 and FN fibrils , and inhibited PDGF BB induced DNA synthesis . 1.1270015^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.83264617
These findings indicate that integrin alpha 5 is rapidly expressed on GC during luteinization , probably under LH stimulation , and suggest that integrin alpha 5 is involved in CL formation via interaction with its ligand FN . . 0.83264617^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.75730794
These results suggest that FN induces apoptosis via its interaction with VLA 5 , and also raise the possibility that the FN VLA 5 interaction may contribute to negative regulation of hematopoiesis . . 0.75730794^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.70092157
Human hematopoietic progenitor cells express very late antigen 4 ( VLA 4 ) and VLA 5 , which mediate their interaction with fibronectin by recognizing the connecting segment 1 ( CS 1 and RGD motifs , respectively . 0.70092157^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
To explore this issue , we previously selected a series of CHO variants that are deficient in expression of the integrin alpha 5 beta 1 , the `` classical ' ' fibronectin receptor . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The surface expression of fibronectin and its major integrin receptor in liver , integrin alpha 5 beta 1 , was studied during liver regeneration after partial hepatectomy . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Complete inhibition of adhesion to fibronectin was observed with anti beta 1 antibody , and partial inhibition with anti alpha 5 , suggesting that integrin alpha 5 beta 1 is mainly responsible for the interaction . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
We have studied the interactions , over several hours , of adult rat hepatocytes with fibronectin substrata in vitro by interference reflection microscopy and by laser scanning confocal immunomicroscopy using antisera against the fibronectin receptors , integrin alpha 5 beta 1 and nonintegrin Agp 110 , together with phalloidin , a specific marker for filamentous actin . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Fibronectin and the fibronectin receptor , integrin alpha 5 beta 1 , were distributed pericellularly and expressed at a steady level during development from the 16th day of gestation and in neonate and adult liver . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Characterization of the integrin alpha 5 beta 6 as a fibronectin binding protein . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Expression of fibronectin , the integrin alpha 5 , and alpha smooth muscle actin in heart and lung development . ^^^ We examined the distribution of FN and the alpha 5 subunit of its receptor , the integrin alpha 5 beta 1 , in the lungs and hearts of murine embryos at 11 , 13 , 16 , and 18 days of gestation . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Insoluble fibronectin activates the Na / H antiporter by clustering and immobilizing integrin alpha 5 beta 1 , independent of cell shape . ^^^ We now show that insoluble fibronectin activates the Na / H antiporter by clustering and immobilizing integrin alpha 5 beta 1 , independent of effects on cell shape . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
This structural difference apparently results in a functional distinction , since purified alpha 5 beta 3 bound to vitronectin , fibrinogen , von Willebrand factor , and fibronectin , whereas integrin alpha 5 beta 5 bound preferentially to vitronectin . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Previous work has shown that adhesion of anchorage dependent cells to fibronectin via integrin alpha 5 beta 1 leads to activation of the Na H antiporter and a rise in intracellular pH ( pHi ) . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Vitronectin receptor antiserum specific for integrin alpha 5 beta 3 blocked cell adhesion and spreading on substrates containing 100 fmol / cm2 of surface bound GRGDY , while fibronectin receptor antiserum specific for alpha 5 beta 1 did not . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Distribution of fibronectin and fibronectin binding proteins , AGp 110 and integrin alpha 5 beta 1 , during chemically induced hepatocarcinogenesis in adult rats . ^^^ We have used single and double label immunocytochemistry to examine the distribution of AGp 110 , integrin alpha 5 beta 1 and fibronectin in adult rat liver during carcinogenesis induced by aflatoxin B 1 or diethylnitrosamine . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Integrin alpha 5 beta 1 is a heterodimeric fibronectin receptor . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
This laminin binding integrin did not bind to Sepharose conjugated with a 105 kDa cell binding fragment of fibronectin and conversely , the fibronectin receptor of the cells ( integrin alpha 5 beta 1 ) did not bind to the laminin Sepharose . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
When human fibroblasts were cultured on fibronectin for 4 h in the presence of 0 . 5 mg / ml of the GRGDSP peptide derived from the fibronectin cell binding site , they adhered and spread normally and organized talin and integrin alpha 5 and beta 1 subunits into focal adhesions . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Antibody mAb 16 , which recognizes the integrin alpha 5 subunit , partially blocked binding of 125I fibronectin and 125I 70 kD . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Transformation of rat 1 , NRK , and Nil 8 cells by Rous sarcoma virus or by murine sarcoma viruses encoding ras oncogenes leads to reductions in the level of integrin alpha 5 beta 1 , which is a well defined fibronectin receptor , and of two other integrin receptors . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
We analyzed the binding of fibronectin to integrin alpha 5 beta 1 in various cells ; in some cells fibronectin bound with low affinity ( e . g . , K 562 cells ) whereas in others ( e . g . , CHO ) , it bound with high affinity ( Kd approximately 100 nM ) in an energy dependent manner . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Endocytosis of integrin alpha 5 beta 1 ( fibronectin receptor ) of mouse peritoneal macrophages in vitro : an immunoelectron microscopic study . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Integrin alpha 5 beta 3 differentially regulates adhesive and phagocytic functions of the fibronectin receptor alpha 5 beta 1 . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The interaction between the central cell binding domain ( CBD ) of fibronectin ( FN ) and its receptor integrin alpha 5 beta 1 was analyzed by both ligand binding and cell adhesion assays . ^^^ The present study suggests that the alpha 5 subunit does not participate in direct binding to the Arg Gly Asp site in CBD when cells adhere to FN through the integrin alpha 5 beta 1 , but that it is involved in the interaction with the synergistic regions of CBD , which then enhances the binding of the beta 1 subunit to the Arg Gly Asp sequence containing CBD . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Synchronous appearance of fibronectin , integrin alpha 5 beta 1 , vinculin and actin in epithelial cells and fibroblasts during rat tracheal wound healing . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The integrin alpha 5 beta 3 binds promiscuously to cell adhesive proteins : vitronectin , fibronectin , and several others containing the RGD motif . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Consequently , we have used this model system to study the effects of repression of autocrine TGF beta 1 activity on the expression of integrin alpha 5 beta 1 and integrin alpha 5 beta 1 mediated cell adhesion to fibronectin . ^^^ The diminished expression of integrin alpha 5 beta 1 on the cell surface led to the reduced adhesion of TGF beta 1 antisense transfected cells to fibronectin . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
To examine whether IL 6 promotes cell attachment through up regulation of expression of integrin alpha 5 beta 1 , i . e . , the major cell surface fibronectin receptor , we quantified the levels of both alpha 5 and beta 1 subunit transcripts by reverse transcription polymerase chain reaction in cultured rabbit corneal epithelial cells pretreated with various concentrations of IL 6 . ^^^ These results indicate that the increased attachment of corneal epithelial cells to fibronectin and enhanced ex vivo migration on corneal stroma by IL 6 is , at least in part , due to the temporal up regulation of integrin alpha 5 beta 1 expression in corneal epithelial cells . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The cytoplasmic domain of the integrin alpha 5 subunit was required for the alpha 5 beta 1 mediated cell survival on fibronectin . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Requirement for the synergy site for cell adhesion to fibronectin depends on the activation state of integrin alpha 5 beta 1 . ^^^ We investigated the influence of the activation state of integrin alpha 5 beta 1 on its dependence on the PHSRN synergy site for binding to RGD in fibronectin . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Integrin alpha 5 beta 1 expression negatively regulates cell growth : reversal by attachment to fibronectin . ^^^ These findings demonstrate that integrin alpha 5 beta 1 expression in the absence of attachment to fibronectin activates a signaling pathway leading to decreased cellular proliferation and that ligation of this receptor with fibronectin reverses this signal , thereby contributing to the proliferation of transformed cells . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Regulation of integrin alpha 5 beta 1 fibronectin interactions by divalent cations . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The increased integrin alpha 5 beta 1 appears to function by reducing mitogenic activity since blockade of fibronectin binding to its receptor with a RGD peptide , a monoclonal anti fibronectin antibody , or a monoclonal anti alpha 5 subunit antibody during the release from quiescence significantly stimulated DNA synthesis . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Tissue specimens were collected at different times after injury and stained with antibodies against subunits of the fibronectin receptor , integrin alpha 5 beta 1 , and the vitronectin receptor , integrin alpha 5 beta 5 . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The integrin alpha 5 beta 6 is expressed primarily in epithelial cells and mediates attachment to the extracellular matrix protein fibronectin . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Role of the integrin alpha 5 beta 6 in cell attachment to fibronectin . ^^^ The integrin alpha 5 beta 6 has been shown to be a fibronectin binding protein . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Affinity modulation of integrin alpha 5 beta 1 : regulation of the functional response by soluble fibronectin . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
However , functionally blocking antisera directed against the vitronectin receptor , integrin alpha 5 beta 3 , and the fibronectin receptor , integrin alpha 5 beta 1 , did not affect HFF spreading on these substrates . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Because fibulin co distributes with both FN and the FN receptor , the integrin alpha 5 beta 1 , we examined the possibility that fibulin was interacting with both components . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Purified FN , antibodies to the integrin alpha 5 beta 1 ( FN receptor on human placenta ) , and antibodies specific for the integrin beta 1 subunit recognized a C . tropicalis membrane protein of 125 + / 25 kDa on immunoblots . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Alendronate inhibited the cAMP increase induced by ligands that primarily utilize integrin alpha nu beta 3 ( vitronectin , Peptite 2000 ) , but not by fibronectin which can also use integrin alpha 5 beta 1 . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Our data indicated that , while talin , vinculin , paxillin , fibronectin receptor and integrin beta 1 were present at the three stress fiber membrane association sites , vitronectin receptor and integrin alpha 5 were absent from the apical plaque and the lateral association site . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Integrin alpha 5 beta 1 clustering was changed in the sites of focal contacts , most probably representing its relocalization as a consequence of the increased amounts of fibronectin . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Integrin alpha 5 beta 1 / alpha 5 beta 3 mediated adhesion to fibronectin , CD 44 mediated adhesion to hyaluronate , and E cadherin / beta 1 integrin mediated adhesion to keratinocytes were not affected by PMA . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Intrablastocoelic injections of several probes including antibodies to FN or integrin alpha 5 beta 1 , competitive peptides to the major cell binding site of FN or the antiadhesive protein tenascin all block mesodermal cell migration . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
This inhibitory effect of the P 3 peptide seems to be specific for alpha 6 beta 1 , since it did not affect the binding of fibronectin to integrin alpha 5 beta 1 . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Identification of a new biological function for the integrin alpha 5 beta 3 : initiation of fibronectin matrix assembly . ^^^ Although the integrin alpha 5 beta 1 has been implicated in the assembly of fibronectin matrices , fibroblastic cells derived from alpha 5 integrin null mutant embryos assemble a fibronectin matrix . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The integrin alpha 5 beta1 mediated fibronectin adhesion , demonstrating for the first time the existence of a functionally active beta 1 integrin on mouse PMN . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The relatively high expression of integrin alpha 5 beta 1 in Group 1 cell lines resulted in strong enhancement of cell adhesion to fibronectin ( FN ) tissue culture plates , whereas Group 3 cell lines showed little or no enhancement of cell adhesion by coating . ^^^ Cell adhesion to FN in Group 1 cells was mainly mediated by integrin alpha 5 beta 1 because a monoclonal anti alpha 5 subunit antibody could block cell adhesion to FN , whereas anti alpha 2 and anti alpha 3 antibodies had no effect on cell adhesion to FN . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Immunohistochemical staining showed that fibronectin was detected in the extracellular matrix ( and partially on the surface of tumor cells ) , integrin alpha 5 beta 1 on the cell surface , and vinculin and actin were detected in the cytoplasm of the tumor cells . ^^^ We conclude that the expression of fibronectin , integrin alpha 5 beta 1 , vinculin , cytoplasmic actin and fibronexus like structure in leukemic cells indicate these cells possess an adhesive function . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The degree of expression of the integrin alpha 5 subunit correlated well with the strength of glioma cell adhesion to fibronectin rather than that of migration to the molecule . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The cell spreading activity of EDA+ FN was not affected by antibodies recognizing the EDA segment but was abolished by antibodies against integrin alpha 5 and beta 1 subunits and by Gly Arg Gly Asp Ser Pro peptide , indicating that the EDA segment enhanced the cell adhesive activity of FN by potentiating the interaction of FN with integrin alpha5beta1 . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
We report that disruption of ligation between alpha 5 alpha 1 and fibronectin by integrin alpha 5 subunit or fibronectin monoclonal antibodies stimulated DNA synthesis in growth arrested FET human colon carcinoma cells . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
To clarify the mechanisms by which substance P ( SP ) and insulin like growth factor 1 ( IGF 1 ) synergistically facilitate corneal epithelial wound healing , we tested the hypothesis that the combination promotes cell attachment to a fibronectin matrix through up regulation of expression of integrin alpha 5 beta 1 , the major cell surface fibronectin receptor in rabbit corneal epithelial cells . ^^^ The combination of SP and IGF 1 significantly increased the number of cells attached to the fibronectin matrix and the expression of integrin alpha 5 . ^^^ Thus , the synergistic enhancing effect of SP and IGF 1 on the attachment of corneal epithelial cells to the fibronectin matrix and on corneal epithelial migration is partly due to the up regulation of integrin alpha 5 expression in corneal epithelial cells . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The expression of integrin alpha 5 beta1 and fibronectin was found to be increased transiently during chondrogenesis . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The cation binding domain from the alpha subunit of integrin alpha 5 beta1 is a minimal domain for fibronectin recognition . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Expression of transforming growth factor beta 1 ( TGF beta 1 ) , the only known inducer of CTGF so far , as well as of the CTGF target genes collagen 1 alpha 1 , fibronectin and integrin alpha 5 revealed a strong correlation with the expression of CTGF . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Arg Arg Glu Thr Ala Trp Ala ( RRETAWA ) is a novel ligand peptide for integrin alpha 5 beta1 , which blocks alpha 5 beta1 mediated cell adhesion to fibronectin ( Koivunen , E . , Wang , B . , and Ruoslahti , E . ( 1994 ) J . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Binding to immobilized anti CD 44 antibody induced a monolayer morphology which is accompanied by fibronectin production and secretion , and expression of the integrin alpha 5 beta 6 . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The combination of both FGLM and IGF 1 and substance P and IGF 1 significantly increased the number of cells adhered to the fibronectin matrix and the expression of integrin alpha 5 . ^^^ The synergistic effect of FGLM and IGF 1 on the adhesion of HCE cells to the fibronectin matrix is partly carried through up regulation of integrin alpha 5 expression in HCE cells . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Expression and detection of integrin alpha 5 beta 1 , fibronectin and its fragment in patients with hepatocellular carcinoma ] . ^^^ OBJECTIVE : To study the relationship between the expression of integrin alpha 5 beta 1 , fibronectin ( FN ) and its fragment ( malignant disease associated DNA binding protein 2 MAD 2 ) and the biologic behavior of human hepatocellular carcinoma HCC ) as well as the significance of MAD 2 detection in plasma . ^^^ METHODS : Integrin alpha 5 beta 1 , FN and MAD 2 were localized by immunohistochemical methods and quantified by image analysis in tumor and peripheral liver tissues from 40 HCC cases . ^^^ RESULTS : The expression of integrin alpha 5 beta 1 , FN and MAD 2 in well differentiated HCC were not significantly different from that of normal tissues , while they were markedly decreased or undetectable in mode rately and poorly differentiated HCC . ^^^ Decreased expression of integrin alpha 5 beta 1 and FN at sites where HCC invaded the connective tissue and enhanced expression on the HCC cells around cancerous emboli were also noted . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
This suppressive effect was accompanied by down regulation of alpha smooth muscle actin , EDA spliced form of fibronectin , and integrin alpha 5 . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The association of fascin with actin in fibronectin adherent cells was also evident in the presence of an inhibitory antibody to integrin alpha 5 subunit . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Human Tenon ' s capsule fibroblasts were cultured into 96 well plates and treated with different concentrations ( ranging from 10 ( 6 ) to 1 microg ml ( 1 ) ) of three different integrin antibodies : human integrin alpha 2 antibody , human integrin alpha 3 antibody and human integrin alpha 5 / FnR ( fibronectin receptor ) antibody . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
On uncoated or fibronectin coated plastic , the integrin alpha 5 and control ( vector only ) transfectants grew at similar rates . ^^^ However , when cultured on fibronectin , the integrin alpha 5 transfectants were not growth inhibited by mAb 225 . ^^^ The reversal of mAb 225 mediated growth inhibition on fibronectin for the integrin alpha 5 transfectants correlated with activation of the EGFR , activation of MAPK , and expression of proliferating cell nuclear antigen . ^^^ Although EGFR activation occurred when either the integrin alpha 5 transfected or control cells were cultured on fibronectin , coprecipitation of the EGFR with SHC could be demonstrated only in the integrin alpha 5 transfected cells . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Column stability is dependent on the interaction between extracellular fibronectin and integrin alpha 5 beta 1 expressed at the cell surface , both of which are upregulated in this pathway . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Expression of integrin alpha 5 , beta 1 subunit and fibronectin mRNAs in human hepatocellular carcinoma ] . ^^^ OBJECTIVE : To study the biologic significance of the expression of integrin alpha 5 , beta 1 subunit and fibronectin ( FN ) mRNAs in hepatocellular carcinoma ( HCC ) . ^^^ METHODS : Integrin alpha 5 , beta 1 subunit and FN mRNAs were detected by using Northern blot ( alpha 32P labeled cDNA probes ) and in situ hybridization ( Dig labeled cDNA probes ) in 15 cases of HCC , 5 cases of paracancerous cirrhotic liver and 3 cases of normal liver tissue . ^^^ RESULTS : In well differentiated HCC , the level of integrin alpha 5 , beta 1 or FN mRNA expression was similar to those in normal liver and paracancerous cirrhotic liver tissues , but was markedly decreased , even absent in the poorly and moderately differentiated HCC tissues ( P < 0 . 01 , respectively ) . ^^^ The expression of integrin alpha 5 and FN mRNAs was mainly detected in the cytoplasm of HCC cells . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The integrin alpha 5 beta 6 is a fibronectin receptor that is undetectable on normal keratinocytes in situ , but is increased significantly in wound healing and in culture established keratinocytes , suggesting that it may promote changes associated with cell motility . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Occurrence of oligosialic acids on integrin alpha 5 subunit and their involvement in cell adhesion to fibronectin . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
These results indicate that the ECM regulates the induction of ALP expression by AsA in fibroblasts : FN enables them to express ALP in response to AsA through interaction with integrin alpha 5 beta 1 , whereas type 1 collagen fibrils cause the suppression of ALP expression and overcome FN . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Expression of dominant negative form of Ets 1 suppresses fibronectin stimulated cell adhesion and migration through down regulation of integrin alpha 5 expression in U 251 glioma cell line . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The production of basement membrane molecules such as collagen type 4 and several laminins , the interstitial molecules fibronectin and tenascin C , and the cell surface molecules integrin alpha 5 and alpha 6 were quantitated in vitro using ELISA on cultured cells from MyoD ( / ) and wild type mice . ^^^ Differences were observed in the production of fibronectin , tenascin C , collagen type 4 , laminin 1 and integrin alpha 5 between control and MyoD ( / ) myotubes in vitro . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Expression of fibronectin receptor , integrin alpha 5 beta 1 of hepatic stellate cells in rat liver fibrosis . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Like the 24 K fragment , a peptide from the 14th type 3 repeat , termed FNIII 14 , which suppressed the integrin alpha 5 beta 1 mediated adhesion of ST 13 cells to FN , accelerated the adipocyte differentiation by preventing FN fibrillogenesis without direct incorporation into the FN matrix . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
We used cell adhesion assay to estimate the affinity of alpha 5 beta 1 integrin with fibronectin , and analyzed the amount of integrin alpha 5 and beta 1 subunits by performing FACS analysis . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Cell adhesion to extracellular matrix proteins such as poly L lysine and fibronectin was mediated by H . pylori induced expression of integrin alpha 5 . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Integrin alpha 5 and beta 1 were strongly expressed in all groups , but integrin alpha 1 was strongly expressed only in collagen type 1 and fibronectin conjugated alginate beads , and integrin alpha 2 was strongly expressed only in collagen type 2 conjugated alginate beads . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
We have used solution 10 ray scattering to visualize the head region of integrin alpha 5 beta 1 in an inactive ( Ca2+ occupied ) state , and in complex with a fragment of fibronectin containing the RGD and synergy recognition sequences . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Their formation is heavily dependent on interactions between integrin alpha 5 beta 1 and fibronectin . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The formation of stress fibers and the localization of integrin alpha 5 and beta 1 chains ( which together form a fibronectin receptor ) in bovine TM cells were investigated by laser confocal microscopy of cells stained with phalloidin and antibodies to the integrin subunits . ^^^ Furthermore , fibronectin induced the spreading of cells , the formation of actin stress fibers , and the expression of integrin alpha 5 in the collagen gel embedded TM cells . ^^^ Moreover , the formation of actin stress fibers and upregulation of integrin alpha 5 appear to contribute to this permissive effect of fibronectin . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Tissue RNA levels of fibronectin and integrin alpha 5 were significantly higher in the hypoxic condition than under normoxic conditions . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
In contrast , blocking of integrin alpha 5 and beta 1 suppressed adhesion and migration on full length Fn and the 110 kDa Fn fragment . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Syndecan 2 cooperates with integrin alpha 5 beta 1 in cell adhesion to a fibronectin substratum and regulates actin cytoskeletal organization in an expression level dependent manner ; Lewis lung carcinoma derived P 29 cells with high expression form stress fibers , whereas the same tumor derived low expressers , LM 66 H11 cells , form cortex actin [ Munesue , S . , Kusano , Y . , Oguri , K . , Itano , N . , Yoshitomi , Y . , Nakanishi , H . , Yamashina , I . , and Okayama , M . ( 2002 ) BIOCHEM : J . 363 , 201 209 ] . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
In Raldh 2 ( / ) embryos , there was down regulation of TGF beta 1 , fibronectin ( Fn ) and integrin alpha 5 , which was associated with decreased visceral endoderm survival and production of VEGF A , Indian hedgehog ( IHH ) , and bFGF . ^^^ Exogenous provision of RA or Fn to Raldh 2 ( / ) explants in whole mouse embryo culture restored vascular remodeling , visceral endoderm survival , as well as integrin alpha 5 expression and its downstream signaling that controls endothelial growth . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
We have previously shown that invasion of human brain microvascular endothelial cells ( HBMEC ) by meningococci is mediated by bacterial outer membrane protein Opc that binds fibronectin , thereby anchoring the bacterium to the integrin alpha 5 beta 1 receptor on the endothelial cell surface . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Gene expression profiling of MMA 2 revealed a set of genes upregulated by the EP 2 agonist , including several growth promoting and apoptosis protecting genes such as the cyclin D 1 , fibronectin , integrin alpha 5 , AP2alpha , and 14 3 3gamma genes . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Our observations unveil a new mechanism of action against NSCLC for COX 2 inhibitors that relates to regulation of integrin alpha 5 gene expression and , consequently , recognition of extracellular matrices ( i . e . , fibronectin ) by tumor cells . ( c ) 2005 Wiley Liss , Inc . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
In addition , TNF alpha induced metastatic properties including adhesion to fibronectin , mRNA expression of integrin alpha 5 , production of matrix metalloproteinase 9 and invasion were inhibited by gefitinib without affecting cell proliferation . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Expression of integrin alpha 5 , a counter adhesion molecule for fibronectin , was inhibited by the treatment with PPARgamma antagonists . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Expression of integrin alpha 5 and integrin beta 4 and their extracellular ligands fibronectin and laminin in human decidua during early pregnancy and its sex steroid mediated regulation . ^^^ The present study aimed to investigate distribution patterns , staining intensity and sex steroid mediated regulation of integrin alpha 5 ( CD49e ) , integrin beta 4 ( CD49f ) expression and their ligands fibronectin and laminin during decidualization . ^^^ Correlating immunostaining was found between laminin and integrin beta 4 , and between fibronectin and integrin alpha 5 . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Increased expression of genes encoding proteins involved in GAS host cell adherence and internalization ( fibronectin , integrin alpha 5 ) was found as a common response . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Based on these data , we hypothesize that fucoidan inhibits the adhesion of MDA MB 231 cells to fibronectin 1 ) by blocking the protein ' s heparin and cell binding domains , 2 ) by modulating the reorganization of the integrin alpha 5 subunit and 3 ) by down regulating the expression of vinculin . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
These findings suggest that mEMAP 2 blocks MEC adhesion and spreading on fibronectin , via a direct interaction with the integrin alpha 5 beta1 , thus implicating that alpha 5 integrin may be a mediator of mEMAP 2 ' s antiangiogenic function . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
METHODS : The expression indexes of integrin alpha 5 and beta 1 on K 562 cells , the binding capability of K 562 cells to fibronectin ( FN ) , and K 562 cell FN binding blocking induced by integrin alpha 5 and beta 1 antibodies were evaluated by flow cytometry ( FCM ) . ^^^ IFNalpha 2b could not change the expression of integrin alpha 5 and beta 1 on K 562 cells , but improved the binding capability of K 562 cells to FN , which could be blocked by anti alpha 5 and / or anti beta 1 antibodies . ^^^ CONCLUSION : IFNalpha 2b could inhibit the proliferation of K 562 cells through restoring the function of integrin alpha 5 and beta 1 , enhancing binding capability of integrin alpha 5 and beta 1 to FN , and up regulating FAK gene expression . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The SHG 44 cells transfected with antisense cDNA of GalT 5 demonstrated decreased Golgi localization of integrin beta 1 , strengthened the interaction between integrin alpha 5 and beta 1 subunit , and enhanced the adhesion ability to fibronectin and the level of focal adhesion kinase phosphorylation . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Lymphocytes and the H 2 tissue expressed adhesion molecule subunits for the following potential cell cell or cell matrix interactions : alpha 3 beta 1 ( VLA 3 ) to fibronectin , laminin , and collagen ; alpha 4 beta 1 ( VLA 4 ) and alpha 5 beta 1 ( VLA 5 ) to fibronectin ; alpha 6 beta 1 ( VLA 6 ) to laminin ; alpha 4 beta 1 to VCAM 1 ; alpha L beta 2 ( Leu CAMa / LFA 1 ) to CD 54 ( ICAM 1 ) ; CD 44 to fibronectin , collagen , laminin , hyaluronate ; CD 2 to CD 58 ( LFA 3 ) ; and CD 56 ( N CAM ) to CD 56 . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
In contrast , anti very late antigen ( VLA ) 5 mAb antibodies significantly reduced Mn ( 2+ ) mediated PMN adherence to fibronectin , but not to laminin or fibrinogen , demonstrating that VLA 5 mediated PMN adherence to fibronectin , but not to fibrinogen or laminin . ^^^ Activation of PMN with phorbol ester or C5a stimulated VLA 5 mediated adhesion to fibronectin , but the contribution of VLA 5 to the forces mediating adherence could only be detected when CD 18 function was either blocked with mAb , or when CD 18 was congenitally absent . ^^^ These data further confirm the presence of PMN VLA integrins and demonstrate that PMN VLA 5 contributes to stimulated PMN adherence to fibronectin . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Chondrocytes strongly expressed beta 1 and alpha 5 integrin subunits , known to form the classical fibronectin receptor , VLA 5 , strongly . alpha 1 was expressed more weakly and variably ; alpha 3 was expressed by occasional cells only . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
This beta 1 mediated regulatory effect involved both VLA 4 and VLA 5 fibronectin receptors . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
VLA family in rheumatoid arthritis : evidence for in vivo regulated adhesion of synovial fluid T cells to fibronectin through VLA 5 integrin . ^^^ Here we have focused functional studies on VLA 5 as an alternative FN receptor for RA T cells . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Homogeneous marrow stromal cell monolayers capable of supporting proliferation of cocultivated CD34hi cells expressed VCAM 1 and fibronectin ( ligands for VLA 4 and VLA 5 ) as well as ICAM 1 ( ligand for LFA 1 and Mac 1 ) . ^^^ Adhesion blocking experiments indicated that VLA 4 / VCAM 1 , VLA 5 / fibronectin , and beta 2 integrin / ICAM 1 pathways all are important for CD34hi cell attachment to stromal cells . ^^^ In addition , CD34hi cells utilized VLA 4 to adhere to purified VCAM 1 and employed VLA 5 ( and to a lesser extent VLA 4 ) to adhere to purified fibronectin . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Previous studies showed that immobilized fibronectin enhanced anti CD 3 monoclonal antibody ( mAb ) induced T cell proliferation through binding to the integrins VLA 4 and VLA 5 ( alpha 5 beta 1 , CD49e / CD29 ) . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
This response to FN was observed with tumor clones isolated from a subcutaneous metastasis and with primary or metastatic melanomas from different patients , but only when tumor cells expressed the alpha 5 subunit of the FN receptor ( i . e . , VLA 5 ) . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Phenotypical analysis of integrin receptor expression ( particularly FN receptors VLA 4 and VLA 5 ) at the membrane of untreated or PMA treated cells revealed that PMA induced no significant modification of the level of expression of these receptors . ^^^ However , inhibition studies carried out with anti VLA monoclonal antibodies demonstrated that the FN specific adhesion triggered by PKC involved the alpha 5 beta 1 FN specific receptors ( VLA 5 ) . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The VLA 5 integrin was shown to be the predominant receptor for fetal melanocyte attachment to FN , in contrast with neonatal melanocytes in which the very late antigen ( VLA ) 5 , VLA 3 , and alpha 5 integrins each contributed to melanocyte attachment to FN . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
These results suggest that the chemotactic ability to fibronectin positively correlates with the metastatic potential in SN 12 and B 16 cell lines , and that VLA 5 receptor is concerned in the motility of highly metastatic SN 12 C 2 cells to soluble fibronectin . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
VLA 3 and especially VLA 5 mediated Langerhans cell binding to fibronectin through the RGDS sequence of the protein . ^^^ Moreover , VLA 5 and VLA 3 take part in the in vitro Langerhans cell binding to fibronectin , suggesting that in vivo these fibronectin receptors could facilitate Langerhans cell passage throughout the fibronectin network of the dermis before migration to lymph nodes . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Interaction of fibronectin with VLA 5 receptor on CD 4 cells induces the AP 1 transcription factor . ^^^ In addition , anti CD 29 ( integrin beta 1 ) as well as anti VLA 5 ( human fibronectin receptor ) antibodies blocked this CD 4 cell activation in this system . ^^^ In an analysis of the molecular mechanism by which IL 2 message was generated , we showed that a fibronectin VLA 5 fibronectin receptor interaction may contribute an independent signal distinct from the CD 3 pathway of activation by the induction of an AP 1 transcriptional factor . ^^^ Thus the VLA 5 fibronectin receptor on CD 4 cells can play a complementary role in CD 3 TCR mediated signal transduction through its interaction with fibronectin . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Negative staining was obtained with antibodies to the fibronectin receptor VLA 5 and VLA 3 . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
In order to ascertain whether these adhesive proteins are normally expressed in inherited epidermolysis bullosa ( EB ) dermal epidermal junction , we studied the reactivity of MoAb recognizing receptors for VLA 1 ( R for unknown ligand ) , VLA 2 ( R for collagen ) , VLA 3 ( R for collagen , laminin , fibronectin ) , VLA 4 ( R for unknown ligand ) , VLA 5 ( R for fibronectin ) , VLA 6 ( R for laminin ) , VNR alpha , and VNR beta ( R for vitronectin ) on cryostat skin sections from EB patients and normal controls and on cytospins of normal epidermal cell suspensions with indirect immunohistochemical methods . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Fibronectin promotes proliferation of naive and memory T cells by signaling through both the VLA 4 and VLA 5 integrin molecules . ^^^ The augmentation of anti CD 3 stimulated proliferation induced by immobilized fibronectin was also inhibited partially by mAb to either VLA 4 or VLA 5 and completely by a combination of the two mAb . ^^^ These studies indicate that signals are transduced through the fibronectin receptors , VLA 4 and VLA 5 , that augment T cell responses and therefore implicate the extracellular matrix protein fibronectin as an important influence regulating T cell responsiveness in vivo . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Human natural killer cells express VLA 4 and VLA 5 , which mediate their adhesion to fibronectin . ^^^ Very late Ag ( VLA ) 3 , VLA 4 , and VLA 5 , belonging to the beta 1 subfamily of integrins , have been recently identified as receptors for different binding regions of fibronectin ( FN ) . ^^^ Using as inhibitory probes a polyclonal antibody against the beta 1 chain of the human FN receptor , the synthetic peptide GRGDSP , which is able to inhibit cellular adhesion mediated by VLA 5 , the CS 1 fragment , which contains the principal adhesion site in the IIICS domain recognized by VLA 4 , and functional mAb directed against alpha 4 or alpha 5 subunits , we show that both VLA 4 and VLA 5 mediate the adhesion of human NK cells to FN . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Expression of the fibronectin receptor VLA 5 is regulated during human B cell differentiation and activation . ^^^ We examined the expression of VLA 5 , a fibronectin receptor , during human B cell development and activation . ^^^ Binding studies , however , demonstrated that adherence of both pre B REH cells and SKW cells to fibronectin was almost completely inhibited by a monoclonal antibody to VLA 5 alpha . ^^^ In addition , Raji cells , which lack VLA 5 but express VLA 3 and VLA 4 , showed very low level binding to fibronectin . ^^^ This demonstrates that for some B lymphocytes VLA 5 , rather than other possible fibronectin receptors , primarily mediates attachment to fibronectin . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
GPIa IIa ( also known as VLA 2 ) , GPIc IIa ( VLA 5 ) , and GPIc * IIa ( VLA 6 ) are involved , respectively , in platelet adhesion to collagen , fibronectin , and laminin . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Most cell lines showed coexpression of the fibronectin receptors ( VLA 4 and VLA 5 ) and the laminin receptor ( VLA 6 ) . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Coexpression of two fibronectin receptors , VLA 4 and VLA 5 , by immature human erythroblastic precursor cells . ^^^ In this study , we report data showing that immature human erythroblasts express the integrins VLA 4 and VLA 5 and that both these molecules act as fibronectin receptors on these cells . ^^^ We have recently demonstrated that adhesion to Fn of purified human CFU E and their immediate progeny preproerythroblasts was inhibited by antibodies directed against the human fibronectin receptor ( VLA 5 ) . ^^^ Furthermore , monoclonal antibodies recognizing the VLA alpha 4 chain blocked the adhesion of immature erythroblasts to Fn coated surfaces , thus demonstrating that , as VLA 5 , VLA 4 is also a functional Fn receptor on these cells . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Its binding to FN was inhibited by anti FN antibody or a mixture of synthetic peptides corresponding to two different sites of FN , termed the V 10 sequence and the RGDS ( Arg Gly Asp Ser ) sequence , which interact , respectively , with the VLA 4 and VLA 5 FN receptors expressed on T lineage cells . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
VLA 4 binds to the peptide sequence Gly Pro Glu Ile Leu Asp Val Pro Ser Thr ( GPEILDVPST in single letter code ) on the alternatively spliced CS 1 form of FN , whereas VLA 5 binds to an Arg Gly Asp sequence found on all forms of FN . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Costimulation of proliferative responses of resting CD4+ T cells by the interaction of VLA 4 and VLA 5 with fibronectin or VLA 6 with laminin . ^^^ Our studies of T cell proliferation complement concurrent studies of T cell adhesion ; both demonstrate that resting CD4+ human T lymphocytes express the VLA integrins VLA 4 , VLA 5 , and VLA 6 , and can use these receptors to interact with the extracellular matrix ( ECM ) proteins fibronectin ( VLA 4 and VLA 5 ) and laminin ( VLA 6 ) . ^^^ However , coimmobilization with the CD 3 mAb of FN or LN , but not other ECM proteins such as fibrinogen and collagen , consistently results in strong T cell proliferation . mAb blocking studies demonstrate that three VLA integrin receptor / ligand interactions mediate costimulation : VLA 4 / FN , VLA 5 / FN , and VLA 6 / LN . ^^^ VLA 5 dependent binding to FN but not costimulation by FN can be specifically blocked with peptides containing the RGD ( arg gly asp ) tripeptide sequence whereas VLA 4 dependent binding and costimulation can both be efficiently inhibited by a 12 amino acid peptide , LHGPEILDVPST ( leu his gly pro glu iso leu asp val pro ser thr ) , derived from the alternatively spliced IIICS region of FN . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
We previously showed that fibronectin ( FN ) synergized with anti CD 3 in induction of CD4+ T cell proliferation , and that VLA 5 acted as a functional FN receptor in a serum free culture system . ^^^ However , this native FN dependent proliferation was entirely abolished by addition of anti VLA 5 alone . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
While VLA 3 interaction with Fn was often low or undetectable in cells having both VLA 3 and VLA 5 , NCI H 69 cells readily attached to Fn in a VLA 3 dependent manner . ^^^ Both Arg Gly Asp ( RGD ) peptide inhibition studies , and Fn fragment affinity columns suggested that VLA 3 , like VLA 5 , may bind to the RGD site in human Fn . ^^^ However , unlike Fn , both Coll and Lm supported VLA 3 mediated adhesion that was not inhibited by RGD peptide , and was totally unaffected by the presence of VLA 5 . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
VLA 6 ( laminin receptor ) and VLA 5 ( fibronectin receptor ) are expressed mainly by LC , and in this way could subserve LC to leave the epidermal compartment through the basement membrane , to migrate throughout the fibronectin network of the dermis before migrating via the afferent lymphatics to the regional lymph nodes , where they present antigen to T cells . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Because the appropriate reagents that recognize individual mouse VLA proteins were not available , we used the human T cell line Jurkat to demonstrate that T cell binding to collagen , laminin , and fibronectin is mediated largely by VLA 2 , VLA 6 , and a combination of VLA 5 and VLA 4 , respectively . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Finally , CFU E adhesion to Fn was blocked by polyclonal lgG raised against the Fn receptor and by a monoclonal antibody against VLA 5 . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
We describe here the regulated binding of resting CD4+ human T cells to ECM through three VLA integrins : VLA 4 and VLA 5 binding to fibronectin ( FN ) , and a novel pathway of VLA 6 binding to laminin ( LN ) . ^^^ Second , binding is regulated with T cell differentiation ; memory T cells express three to four fold more VLA 4 , VLA 5 , and VLA 6 than do naive cells , and bind more efficiently through them to FN and LN . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
VLA 1 was demonstrated in the glomerular mesangium and VLA 5 , an FN specific receptor , was present in the mesangium on glomerular endothelial cells and on MC . ^^^ In addition , anti VLA 5 but not anti VLA 3 antibodies inhibited significantly the binding of MC to FN , MC demonstrated binding and phagocytosis of FN coated beads and , purified FN inhibited both phenomena . ^^^ MC FN receptors ( VLA 5 ) mediate the binding of MC to FN and could mediate the phagocytosis of FN coated antigen or immune complexes by mesangial cells . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Such embryos have defects in FN synthesis and gastrulation . ( b ) Fab ' fragments of anti FN and anti integrin VLA 5 IgGs prohibit mesodermal cell adhesion both in vitro and in vivo and gastrulation is arrested . ( c ) Peptides containing the RGDS sequence specifically inhibit interactions between migrating mesodermal cells and the FN fibrillar matrix . ( d ) Tenascin blocks cell adhesion to FN in vitro and gastrulation in vivo . ( e ) Antibodies against the cytoplasmic domain of beta 1 integrin , when injected into blastomeres , prevent FN fibrillogenesis in progeny of injected blastomeres and delay mesodermal cell migration selectively in the progeny of injected blastomeres but not in the uninjected blastomere progeny . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Except for the identification of VLA 5 as a fibronectin receptor structure , the functions of the VLA proteins have remained unclarified . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Monocytes and T cells reacted with VLA 5 that was previously identified as the human FN receptor , whereas no labeling with anti VLA 5 could be shown for B cells . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
A synergistic effect mediated by the VLA 5 fibronectin receptor complex . ^^^ Furthermore , it is the VLA 5 protein within the set of molecules recognized by anti 4B4 that serves as a fibronectin receptor on the CD 4 lymphocytes . ^^^ The VLA 5 fibronectin receptor was mainly expressed on CD4+ CD45R CDw29+ cells and may in part contribute to the unique function of these cells . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Evidence for distinct VLA 2 , VLA 5 ( fibronectin receptor ) , and VLA 6 structures . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
This paper shows that the common VLA beta subunit is equivalent to subunits found in structures with known fibronectin and laminin receptor activity , and that VLA 3 and VLA 5 are similar or identical to these previously defined receptors for adhesion molecules . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Immunophenotypic analysis of marrow blast cells revealed a significant predominance of the ' null ' phenotype in the CD 34 positive group , together with a strong expression of the VLA 4 and VLA 5 integrins ( fibronectin receptors ) . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The beta 1 subfamily of integrins is thought to play an important role in both the adhesion / migration and proliferation / differentiation of T cells . beta 1 integrins can provide T cell costimulation through interaction of very late antigen ( VLA ) 4 ( VLA 4 ) ( alpha 4 beta 1 ) and VLA 5 ( alpha 5 beta 1 ) with the extracellular matrix protein fibronectin ( FN ) , or by VLA 4 binding to its cell surface ligand , vascular cell adhesion molecule ( VCAM ) 1 . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The presence of VLA 4 as well as VLA 5 on activated eosinophils was confirmed by demonstration of the formation of immune rosettes using antibody coated microspheres and by their attachment to fibronectin coated surfaces . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
These results suggest that perturbation of CD 9 on pre B cells promotes recognition of stromal cell fibronectin by VLA 4 and VLA 5 and implicates CD 9 as a novel regulator of inside out signaling relevant to B lymphopoiesis . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
VLA 5 and fibronectin could mediate adhesion of the erythroid progenitor cells to the stromal cells , but the adhesion itself may not be sufficient for the stroma supported erythropoiesis . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The growth suppression or apoptosis induced by FN was rescued by the addition of either anti FN antibody , anti very late antigen 5 monoclonal antibody ( anti VLA 5 mAb ) , or GRGDSP peptide , but not by that of anti VLA 4 mAb or GRGESP peptide , suggesting that the FN effects on M07E cells were mediated through VLA 5 . ^^^ In addition , the FN induced apoptosis was detectable in VLA 5 positive human hematopoietic cell lines other than M07E cells , but not in any of the VLA 5 negative cell lines . ^^^ These results suggest that FN is capable of inducing apoptosis via its interaction with VLA 5 , and also raise the possibility that the FN VLA 5 interaction may contribute , at least in part , to negative regulation of hematopoiesis . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The cells of the lining layer were strongly ICAM 1+ and VLA 5+ , suggesting ( 1 ) that ICAM 1 may function to facilitate the adhesion of ICAM 1 bearing type A cells to type B cells , and ( 2 ) that the lining cells may utilize VLA 5 for anchorage to fibronectin at the surface of the synovial membrane . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
In contrast , treatment of stromal cells with antibodies to vascular cell adhesion molecule or fibronectin ( ligands of VLA 4 and VLA 5 ) did not prevent leukemic cell migration . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
They adhered to fibronectin through VLA 5 when stimulated with steel factor and also directly to membrane anchored steel factor through c kit . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
VLA 4 , VLA 5 , and VLA 6 activated with either mAbs or their natural ligands ( fibronectin , laminin , and vascular cell adhesion molecule 1 ) are able to transduce costimulatory signals in thymocytes activated via the CD 3 pathway , i . e . , enhancement of thymocyte proliferation , CD 25 and CD 69 expression , and IL 2 secretion . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Inhibition studies with monoclonal antibodies revealed that this binding involves the VLA 4 / VCAM 1 and VLA 5 / fibronectin ( FN ) , but not the VLA 4 / CS1 pathway . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
We confirm that the integrins very late antigen ( VLA ) 4 and VLA 5 are expressed on the CD34+ cell lines MO7e , TF 1 , and on normal bone marrow CD34+ progenitor cells , but in a low affinity state , conferring on them a weak adhesive phenotype on fibronectin ( Fn ) . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
In contrast , VLA 1 , VLA 2 , and VLA 6 integrins were not expressed significantly . mAb to VLA 3 , VLA 4 , and VLA 5 each inhibited by 70 % skin mast cell adhesion to fibronectin . mAb to VLA 3 nearly abolished mast cells adhesion to laminin , whereas anti VLA 4 and anti VLA 5 were ineffective . ^^^ Our data demonstrate that human skin mast cells spontaneously adhere to fibronectin and laminin , and that this adhesion is mediated by VLA 3 , VLA 4 , and / or VLA 5 integrins on these cells . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Cytokine network plays an essential role for immune and inflammatory reaction , and FN has been shown to induce monokines through VLA 5 receptor on cultured monocytes . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Soluble fibronectin , which is the natural ligand for VLA 5 , or mAbs against VLA 5 inhibited binding to monocytes of B . pertussis strains that express FimD but not of mutant strains that lack FimD . ^^^ Beads that were coated with the fusion protein maltose binding protein FimD bound to adherent monocytes , and this binding was inhibited by soluble fibronectin or mAb against the alpha or beta chain of VLA 5 , while soluble collagen or mAb against VLA 4 , VLA 6 , CR 3 , or HLA class 2 had no effect . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Incubation of fluid phase PMN with anti VLA 5 ( alpha 5 / beta 1 ) and anti VLA 6 ( alpha 6 / beta 1 ) mAbs blocked the effect of fibronectin and laminin on CD 16 and CD11b / CD18 expression . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NK cells bind to fibronectin and laminin using the beta 1 integrins VLA 4 and VLA 5 , and VLA 6 respectively . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Nevertheless , anti alpha 5 MoAb completely inhibited chemotaxis when it was added with anti alpha 4 in FR4ds , demonstrating a novel complementary role of VLA 5 toward VLA 4 in the chemotaxis induced by FN . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
However , 5 delta 1+ cells exhibited higher levels of VLA 5 that correlated with an increased adhesiveness to fibronectin and to a 120 kDa fibronectin fragment ( FN 120 ) that contains only the VLA 5 binding domain but not to type 1 collagen or to a fibronectin fragment ( FN 40 ) that binds only VLA 4 . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Interaction of the cell binding domain of fibronectin with VLA 5 integrin induces monokine production in cultured human monocytes . ^^^ These results indicate that fibronectin induced production of IL 1 alpha , IL 1 beta , TNF alpha and IL 6 from cultured monocytes is mediated predominantly by interaction of the cell binding domain of fibronectin with VLA 5 , although Mac 1 also may contribute to this effect of fibronectin . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Only J11d+ thymocytes ( 12 to 15 % ) bound to fibronectin ( via VLA 4 and VLA 5 ) and binding to laminin ( via VLA 6 ) was two to fourfold higher in J11d+ compared with J11d thymocytes ( 60 to 80 % vs 20 to 30 % ) . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Both VLA 4 and VLA 5 fibronectin ( FN ) , as well as VLA 2 collagen ( COL ) receptors mediated cell spreading and morphological changes . ^^^ Furthermore , the beta 1 regulated cell spreading on VCAM 1 and COL took place independently of the VLA 5 FN receptor function . ^^^ The tyrosine phosphorylation of polypeptides of 130 kDa and 77 kDa was triggered in U 937 cells by the interaction of FN with the VLA 5 receptor in a high affinity conformation . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Analysis of binding mechanisms indicated that very late antigen 4 ( VLA 4 ) and VLA 5 were responsible for AML cell binding to fibronectin . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Attachment of fibroblasts to collagen and fibronectin is dominated by specific integrin binding : only few cells were able to attach to collagen after inhibition with an anti VLA 2 antibody , or to attach to fibronectin after inhibition with an anti VLA 5 antibody . ^^^ On unexposed sites , cells from old donors showed a significantly increased adhesion capacity on collagen ( plus 50 . 7 % ) and on fibronectin ( plus 62 . 4 % ) and an increased staining pattern of VLA 2 and VLA 5 integrins in immunohistochemistry in comparison with young donors . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
In the present report , we studied the effects of LPS on the expression of VLA 5 ( alpha 5 beta 1 ) , VLA 3 ( alpha 3 beta 1 ) , and VLA 2 ( alpha 2 beta 1 ) integrins and fibronectin ( FN ) by human alveolar macrophages in an attempt to understand the mechanism by which LPS regulates macrophage adhesion to matrix proteins . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
These mast cells expressed fibronectin receptor integrins , including very late antigen ( VLA ) 4 , VLA 5 and vitronectin receptor ( VNR ) , as estimated by immunofluorescent staining and inhibition of FN adherence by newly established mAbs reactive with the rat alpha 4 ( MR alpha 4 1 ) , alpha 5 ( HM alpha 5 1 ) or beta 3 ( HM beta 3 1 ) chains of the integrin molecules . ^^^ This FN enhancement of beta hexosaminidase release was inhibited by soluble MR alpha 4 1 , HM alpha 5 1 and HM beta 3 1 as well as by GRGDSP and DELPQLVTLPHPNHLGPEILDVPST peptides which abrogate VLA 5 / VNR and VLA 4 binding to FN respectively . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Although many receptors mediating the effects of ECM components on ligament cell function remain poorly defined , it is known that fibronectin ( FN ) allows ligament cells to adhere through the VLA 5 receptor ( alpha 5 beta 1 ) . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Both the random and chemotactic migration were dependent on fibronectin receptors VLA 4 and VLA 5 on T cells . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Effects of glycated protein on the expression of very late antigen 5 ( VLA 5 ) , a fibronectin receptor , of cultured rat mesangial cells . ^^^ In the present study , 1 assessed cultured rat mesangial cells for the presence of mRNA for VLA 5 , one of the fibronectin receptors . ^^^ Using these cells , 1 also evaluated the effects of glycated proteins on the expression of VLA 5 and fibronectin . ^^^ Immunostaining and Northern blot analyses showed that the cells incubated with AGE BSA exhibited a decrease in VLA 5 protein and related mRNA , but showed an increase in the synthesis of fibronectin and related mRNA . ^^^ On the other hand , non glycated BSA did not induce these changes in the expression of VLA 5 or fibronectin in the mesanginal cells . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Both LTBMC adherent cells from myeloma and normal donor origin expressed CD 10 , CD 13 , the adhesion molecules CD 44 , CD 54 , vascular cell adhesion molecule 1 , very late antigen 2 ( VLA 2 ) , and VLA 5 , and were positive for extracellular matrix components fibronectin , laminin , and collagen types 3 and 4 . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Cell adhesion to fibronectin before and after treatment with BZ alpha GalNAc or TPA was inhibited by anti VLA 4 and anti VLA 5 monoclonal antibodies . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Platelet adhesion to fibronectin in flow : dependence on surface concentration and shear rate , role of platelet membrane glycoproteins GP IIb / IIIa and VLA 5 , and inhibition by heparin . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Parallel analysis of other integrin subunits mRNA ( beta 1 , alpha 5 , beta 7 ) demonstrated that the mRNA levels for the alpha subunits of the fibronectin receptors alpha 4 / beta 1 ( VLA 4 ) and alpha 5 / beta 1 ( VLA 5 ) are differentially regulated during the monocytic differentiation of myeloid cell lines , and suggested that myeloid cells express a heterodimer formed by the association of beta 7 with an integrin alpha subunit distinct from alpha 4 . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Other adhesion receptors include members of the VLA subclass of the integrin family : VLA 2 , VLA 5 and VLA 6 , which mediate platelet adhesion to collagen , fibronectin and laminin , respectively . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Flow cytometric analysis showed that both peripheral blood lymphocytes and the LAK cells express fibronectin receptors VLA 3 , VLA 4 and VLA 5 on their surfaces . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Furthermore , a mAb against the VLA 5 integrin ( VLA 5 ; Fn receptor ) also inhibited TGF beta induced chemotaxis . ^^^ These results indicate that chemotaxis of PMNs in response to TGF beta isoforms is mediated by the interaction of the Arg gly Asp ser sequence in the CBD of Fn with an integrin on the PMN cell surface , primarily the VLA 5 integrin . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
FN in the culture medium ( 200 micrograms / ml ) induced strong monokine production that was mediated by the domain epitope including RGD sequence on FN and the VLA 5 integrins on monocytes . ^^^ These results indicate that monocytes activation in vitro may depend on not only monokines but on adhesion molecules such as FN VLA 5 . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Blocking the classical fibronectin receptor with antibody to VLA 5 alpha gave a slightly lower inhibition at 42 % . ^^^ In normal peripheral lymph nodes , the synthetic peptide CS 1 and antibodies to fibronectin and VLA 5 also partially inhibited T cell binding to HEVs ( 45 , 47 , and 52 % respectively ) . ^^^ CONCLUSION These results show that fibronectin mediates lymphocyte HEV interactions not only through its classical VLA 5 receptor , but also through its CS 1 adhesion motif in inflamed synovium and peripheral lymph nodes . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
It was found that soluble fibronectin and soluble mAb against VLA 5 markedly inhibited the attachment of B . pertussis to monocytes . ^^^ When VLA 5 on monocytes was cross linked by plating these cells onto surfaces precoated with fibronectin or mAb against VLA 5 , the binding of both B . pertussis and C3bi coated sheep erythrocytes to these cells was significantly enhanced , whereas the binding of a B . pertussis mutant strain deficient in filamentous hemagglutinin was not affected . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
HCs expressed abundant VLA 5 , an FN receptor not present on normal circulating B lymphocytes , but important in matrix formation . ^^^ Furthermore , HCs were shown to adhere to an FN fragment containing the VLA 5 binding site . ^^^ It is therefore suggested that the VLA 5 of HCs is implicated in their assembly of FN matrix . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
In addition , a much weaker expression of the second fibronectin receptor VLA 5 , the laminin receptor VLA 6 and the vitronectin receptor CD 51 was demonstrated . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Interestingly , mast cells were positive for VLA 3 and VLA 5 , both of which are receptors for fibronectin , indicating that these integrins play an important role in mast cell function . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Antibodies to CD11a , CD 18 , VLA 4 alpha , and VLA 5 alpha failed to inhibit binding of AML cells to bone marrow fibroblasts but anti VLA 5 alpha antibodies inhibited AML cell binding to fibronectin by approximately 50 % . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The integrin VLA 2 as a collagen receptor and VLA 5 as a fibronectin receptor were detected immunohistochemically in normal , benign tumor and carcinoma tissues of the breast . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
In view of the known binding of VLA 4 and VLA 5 to extracellular fibronectin ( FN ) , the adhesion of leukaemic cells to FN was evaluated in a colorimetric assay . ^^^ These studies indicated that ALL cells adhere to soluble fibronectin predominantly through the VLA 5 molecule ( blockable with the PHM 2 antibody and a peptide containing the RGD sequence ) although binding mediated by VLA 4 was also apparent in some experiments ( blockable by a 40 kDa fragment containing the heparin binding domain of FN and inhibitory antibodies ) . ^^^ These results indicate that precursor B ALL cells may adhere to marrow stroma through interaction of VLA 4 and VLA 5 with FN , although other mechanisms of adhesion may be important . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
These interactions of the lymphocytes with 3H labelled FN in solution could be almost completely blocked by monoclonal anti VLA 4 and VLA 5 antibodies . ^^^ Although both freshly purified and MLC activated lymphocytes have equal expression of the integrins VLA 4 and VLA 5 , only activated cells are capable of ' spontaneous ' binding FN in solution via an integrin mediated process , probably via an increase in the affinity of these receptors for FN . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
These peptides blocked the interaction of the integrins VLA 4 and VLA 5 with fibronectin ( FN ) , and our results suggested that by preventing the interaction of T cell integrins with FN , we successfully prevented the migration of T cells to sites of antigenic challenge . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Finally , mAb specific for the alpha 4 and alpha 5 subunits of the two major Fn binding molecules of macrophages ( VLA 4 and VLA 5 , respectively ) , either alone or in combination , were poor inhibitors of T . cruzi uptake , suggesting that several members of the VLA family , including VLA 4 and VLA 5 , are involved in binding and entry of T . cruzi into macrophages . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Fibronectin receptors , VLA 4 or VLA 5 , were expressed at various levels on all myeloma cell lines . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
To quantitate the effect of the regulatory anti beta 1 TS2 / 16 mAb on the affinity of VLA 5 for its ligand , an RGD containing fragment of fibronectin ( FN 80 ) , we performed binding studies of radiolabeled soluble FN 80 to U 937 cells . ^^^ The yield of VLA 5 fibronectin receptor bound to FN 80 Sepharose columns was strongly increased upon treatment of U 937 cell lysates with mAb TS2 / 16 . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Adhesion and costimulation of proliferative responses of human gamma delta T cells by interaction of VLA 4 and VLA 5 with fibronectin . ^^^ The very late antigens , VLA 4 and VLA 5 belong to the beta 1 subfamily of integrins and have been identified as receptors for different binding regions of fibronectin ( FN ) . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Recently we found that steel factor and c kit induce adhesion to fibronectin by VLA 5 in mast cells . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The expression of beta 1 integrins in IgA nephropathy was similar to that of healthy individuals , but the function of these molecules in terms of adhesion to fibronectin though VLA 4 and VLA 5 is increased in these patients . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Here we demonstrate that VLA 5 , an RGD specific fibronectin receptor on a wide variety of cell types , can bind to murine L 1 . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
This adherence was partially inhibited by antibodies specific for plasma FN , VLA 4 , VLA 5 , and VLA beta 1 , and by the synthetic peptide RGDS . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
HTSC express CD 54 ( ICAM 1 ) , CD49a ( VLA 1 ) , CD49b ( VLA 2 ) , CD49c ( VLA 3 ) , CD49e ( VLA 5 ) , CD49f ( VLA 6 ) , CD 29 , CD 51 , CD 44 and produce vinculin , beta tubulin , alpha actin , vimentin , fibronectin , laminin and collagen types 1 , 3 and 4 . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
It is generally accepted that the interaction of cells with FN is mediated by several integrins such as alpha 5 beta 1 ( VLA 5 ) or alpha 4 beta 1 ( VLA 4 ) , and analysis of flow cytometry showed that 185 4 cells express these integrins on their surface . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Interaction between fibronectin ( FN ) and very late activation Ag 5 ( VLA 5 ) integrin was recently reported to be involved in apoptosis of hematopoietic cells . ^^^ In an effort to clarify the physiologic role of FN in the regulation of biologic behavior of terminally differentiated hematopoietic cells , we have examined the change of VLA 5 expression during myeloid cell differentiation and its effects on monocytes and granulocytes . ^^^ Susceptibility to FN induced apoptosis was greatly increased upon monocytic differentiation , and it was almost completely abrogated by anti VLA 5 Ab or RGD peptide . ^^^ These results suggest that FN might play an important role in negative regulation of the survival of monocytes through its interaction with VLA 5 , which is selectively up regulated during monocytic differentiation . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Furthermore , tat expressing cells produce more fibronectin and display upregulation of VLA 5 cell surface receptor levels compared to control cells , while alpha 5 expression was unchanged . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
MFR 5 , a monoclonal antibody which selectively blocks function of the alpha 5 chain of the alpha 5 beta 1 integrin , VLA 5 , decreased the chemotactic response toward fibronectin of ND cells by 37 % ; the chemotactic response by LTP cells was reduced by 49 % . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The MCP 1 induced binding of T lymphocytes to FN was rapid , dose dependent , and resulted from activation of both VLA 4 and VLA 5 . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
In the same model , we determined herein that the expression of VLA 5 and VLA 6 ( fibronectin and laminin receptors , respectively ) were upregulated by low doses of IFN gamma , whereas high doses of the cytokine induced an opposite effect . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Immunogold labelingsoliduselectron microscopy analysis revealed that the areas of contact between lymphoblasts and fibroblasts contained beta 1 integrins ( VLA 4 and VLA 5 ) , fibronectin , vascular cell adhesion molecule ( VCAM 1 ) , and a cartilage link protein , CD 44 . ^^^ Double immunogold labeling studies disclosed a direct in situ relationship between fibronectin and VLA 4 , VLA 5 , and CD 44 . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Cell adhesion and migration are regulated at distinct stages of thymic T cell development : the roles of fibronectin , VLA 4 , and VLA 5 . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
We have previously shown that normal CD34+ HPCs express high levels of fibronectin ( Fn ) receptors very late antigen 4 ( VLA 4 ) and VLA 5 in a low affinity state , which do not allow HPCs to strongly adhere on immobilized Fn , and that cytokines such as interleukin 3 , granulocyte monocyte colony stimulating factor , and stem cell factor transiently activate these receptors , providing HPCs with an adhesive phenotype on Fn . ^^^ Therefore , we propose a 2 step model with an initial activation of VLA 4 and VLA 5 generated by cytokine receptors that is followed by a secondary signal resulting from Fn binding to VLA 4 and VLA 5 , which may cooperate with those generated by cytokine receptors . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
A host protein present in the extracellular matrix , fibronectin , has binding activities similar to those of B . pertussis fimbriae , binding to both Vla 5 and sulfated sugars . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Further immunohistochemistry showed the presence of cell adhesion molecules such as fibronectin , VLA 2 , VLA 5 , and VLA 6 , the vitronectin receptor , 1CAM 1 , LFA 1 , and the PDGF receptor B . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The effect was due to enhanced function of the VLA 5 integrin fibronectin receptor and not to increased surface expression . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Adhesion assays on purified vascular cell adhension molecule ( VCAM ) 1 , fibronectin , and fibronectin fragments revealed distinct kinetic patterns for the regulation of very late antigen ( VLA ) 4 ( alpha 4 beta 1 ) and VLA 5 ( alpha 5 beta 1 ) avidity by the CC chemokines monocyte inflammatory protein ( MIP ) 1 alpha , RANTES ( regulated on activation , normal T expressed and secreted ) , or monocyte chemoattractant protein ( MCP ) 1 in monocytes . ^^^ Controlled detachment assays in shear flow suggested that adhesive strength of VLA 4 for VCAM 1 or the 40 kD fragment of fibronectin ( FN 40 ) is more rapidly increased and subsequently reduced by MCP 1 than by MIP 1 alpha , and confirmed late and sustained activation of the adhesive strength of VLA 5 for the 120 kD fragment of fibronectin ( FN 120 ) . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
An antibody to the alpha chain of VLA 3 mimicked the effect of the 120 kD FN , whereas an antibody to the alpha chain of VLA 5 was partly inhibitory . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
We examined the expressions of alpha subunits of very late antigen ( VLA ) 4 ( alpha 4 ) and VLA 5 ( alpha 5 ) on tonsillar lymphocytes and the interaction between these integrin receptors and their ligand , fibronectin ( FN ) . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Together with mAb R 1 2 , we characterized the expression of VLA 4 ( alpha 4 beta 1 ) and VLA 5 integrins , the two major fibronectin receptors , on two long term cultured mast cell lines , CFTL 15 and MC / 9 . ^^^ During the first 3 weeks the expression of VLA 4 and VLA 5 increased progressively and both were involved in mediating adhesion of BMMC to fibronectin . ^^^ In comparison , VLA 5 remained stably expressed and functioned as the major receptor for fibronectin . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Furthermore , the immunochemical evidence that FDCs were fibronectin ( VLA 5 , CD49e / 29 ) and laminin ( VLA 6 , CD49l / 29 ) receptor positive discussed above was confirmed by immunoelectron microscopy and binding assays . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
VLA 5 in the plasma cell line , FR4ds , acts as a common regulator of VLA 4 and VLA 6 in spreading induced by fibronectin and laminin . ^^^ VLA 5 recognizes the GRGDSP sequence of fibronectin ( FN ) in the extracellular matrix ( ECM ) . ^^^ We first examined the role of VLA 5 in the spreading induced by FN . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Of the three cultures , only two , SK N AS and GL 15 , had a complete response to TGFbeta , with induction of the following effects : ( 1 ) inhibition of cell proliferation ; ( 2 ) up regulation of the extracellular matrix glycoprotein fibronectin , together with down regulation of the VLA 5 integrin receptor ; ( 3 ) up regulation of histotype specific cytoskeletal intermediate filaments ( neurofilaments for neuroblastoma and GFAP for glioblastoma ) ; and ( 4 ) increase in the glycoprotein CD 44 , only in SK N AS . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Stimulation of monocyte TFA may be more dependent on interactions between monocytes and the vegetational surface via fibronectin receptors , such as VLA 4 and VLA 5 ( very late antigens 4 and 5 ) . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
We demonstrate herein that the expression of the epitope recognized by mAb HUTS 21 is induced on T lymphoblasts upon binding of soluble vascular cell adhesion molecule ( VCAM ) 1 and an 80 kDa tryptic fragment of fibronectin ( FN 80 ) to the beta 1 integrins very late activation antigen ( VLA ) 4 and VLA 5 , and that this effect is dependent on ligand concentration and is specific for beta 1 integrins . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Adhesion to FN was mediated by beta 1 integrins alpha 4 beta 1 ( VLA 4 ) and alpha 5 beta 1 ( VLA 5 ) since blocking antibodies against beta 1 ( CD 29 ) , alpha 4 ( CD49d ) , or alpha 5 ( CD49e ) integrin subunits , completely reversed the effect of HGF / SF . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
In this study , we demonstrated that immobilized fibronectin ( FN ) enhanced lymphokine activated killer cell ( LAK ) activity , and that the enhanced LAK activity was completely abrogated by an anti VLA 5 monoclonal antibody and RGD peptide . ^^^ LAK cells showed increased adhesion to immobilized FN compared with that to control bovine serum albumin , and the increased adhesion of LAK cells to immobilized FN was inhibited by anti VLA 5 monoclonal antibody . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The interaction between beta 1 integrin ( VLA 5 ) and extracellular matrix protein ( fibronectin ) activation of cultured monocytes by fibronectin ] . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Haemopoietic progenitors also bind to the RGD motif of fibronectin through an interaction with VLA 5 and we therefore also studied the effect of antibodies to VLA 5 on progenitor homing and mobilization . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Binding of human leukocytes to fibronectin is augmented by an anti CD 44 mAb ( TL 1 ) and blocked by another anti CD 44 mAb ( Hermes 3 ) but not by anti VLA 4 / VLA 5 mAbs . ^^^ Lymphoid cells are activated by binding to FN of the VLA 4 and VLA 5 receptors . ^^^ This augmentation was not inhibited by the CS 1 and RGD peptides of FN or by anti VLA 4 and VLA 5 mAbs ; it was energy dependent and associated with cytoplasmic actin filaments . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Treatment of a mouse thymic epithelial cell line with these hormones induced an increase in thymocyte adhesion , an effect significantly prevented in the presence of antibodies to fibronectin , laminin or respective receptors VLA 5 and VLA 6 . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Anti VLA 5 ( CD49e ) but not anti VLA 1 through VLA 4 and VLA 6 , blocked the effect of Fn on TNF alpha receptors , whereas anti VLA 6 but not anti VLA 1 through VLA 5 blocked the effect of Ln on IL 1 beta receptors . ^^^ Modulation of IL 1 beta and TNF alpha receptors by VLA 5 and VLA 6 required protein tyrosine kinase activation as herbimycin A ( 10 micrograms / mL ) blocked the affect of Fn and Ln . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Thrombopoietin promotes adhesion of primitive human hemopoietic cells to fibronectin and vascular cell adhesion molecule 1 : role of activation of very late antigen ( VLA ) 4 and VLA 5 . ^^^ In the present study we demonstrate that Tpo can stimulate the adhesion of the Mo7e progenitor cell line to fibronectin ( Fn ) as well as vascular cell adhesion molecule 1 through activation of very late antigen ( VLA ) 4 and VLA 5 , adhesion molecules previously demonstrated to be involved in regulation of steady state hemopoiesis . ^^^ Finally , Tpo stimulated adhesion of primitive ( CD34+ CD 38 ) human bone marrow cells to fibronectin , predominantly through activation of VLA 5 , whereas no such effect could be observed on CD34+ CD38+ bone marrow cells . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The development of efficient culture techniques for the production of megakaryocytes ( Mks ) from CD34+ purified BM cells , enables the study of the expression and function of adhesion receptors for collagen ( VLA 2 ) , fibronectin ( VLA 4 and VLA 5 ) and laminin ( VLA 6 ) during the maturation of Mks . ^^^ Our data suggest that VLA 4 may be involved in the adhesion of CFU MK and immature Mks on fibronectin , then replaced by VLA 5 in the final stages of maturation . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Incubation with interleukin 6 and stem cell factor enhanced the expression of fibronectin ligands VLA 4 and VLA 5 on CD34+ cells . ^^^ This enhanced expression of VLA 4 and VLA 5 was considered to facilitate a close contact of the CD34+ cell to the retroviral vector via fibronectin fragments and the subsequent transduction process . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Filters were then processed for immunoelectron microscopy using antibodies for alpha smooth muscle ( alpha SM ) actin as a marker for myofibroblasts , cellular fibronectin ( cFn ) and VLA 5 . 3 . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Overexpression of Chk was found to suppress VLA 5 integrin mediated cell spreading , but not cell attachment , throughout fibronectin ( FN ) stimulation . ^^^ These results suggest that sustained activation of Lyn , which is regulated by membrane anchored Chk , plays a crucial role in VLA 5 mediated cell spreading but not cell attachment to a FN substrate . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Fibronectin ( FN ) is supposed to play important roles in various aspects of hematopoiesis through binding to very late antigen 4 ( VLA 4 ) and VLA 5 . ^^^ Fibronectin ( FN ) is supposed to play important roles in various aspects of hematopoiesis through binding to very late antigen 4 ( VLA 4 ) and VLA 5 . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The differential use of VLA 4 and VLA 5 correlated with an increase in fibronectin surface expression by stromal cells in response to TGF beta 1 . ^^^ Adhesion assays to purified fibronectin using Nalm 6 cells showed a predominant utilization of VLA 4 at low concentrations of this ligand , whereas higher concentrations resulted in a preferential use of VLA 5 . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Binding to a natural ECM , derived from cultured EC , or to purified fibronectin was high : 52 % + / 6 % ( n = 8 ) involved VLA 4 and VLA 5 integrins . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
By immunohistochemistry and western blotting using the antibodies against integrin ( VLA 5 ) , talin , vinculin , fodrin , vimentin , ankyrin , plectin , fibronectin , and focal adhesion kinase ( FAK ) , there was no significant difference in localization and amount between the groups . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Interleukin 3 and Flt 3 ligand induce adhesion of Baf3 / Flt3 precursor B lymphoid cells to fibronectin via activation of VLA 4 and VLA 5 . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
VLA 5 is expressed by mouse and human long term repopulating hematopoietic cells and mediates adhesion to extracellular matrix protein fibronectin . ^^^ Here we show that mouse long term in vivo repopulating stem cells , as well as primitive human NOD / SCID mouse repopulating cells , can bind extracellular matrix protein FN by using integrin VLA 5 in vitro . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Baseline and enhanced adhesion occur through the surface FN receptor very late antigen 5 ( VLA 5 , CD49e / CD29 ) which is expressed by AML cells . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Immunofluorescence staining analysis for the fibronectin receptors VLA 4 and VLA 5 showed no expression on injured proximal tubules , whereas the expression of the laminin receptor VLA 6 was increased and irregular on altered PT . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
In this study we investigated the effect of immobilized FN on the proliferation of various B cell lines , including a newly established B cell line , OPM 3 , and human tonsillar B cells , that primarily express VLA 4 but not VLA 5 . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Very late activation antigen ( VLA ) 4 and VLA 5 are two major ligands to FN on thymocytes . ^^^ Furthermore , the enhancing effect by FN was inhibited in the presence of the Gly Arg Gly Asp Ser Pro ( GRGDSP ) peptide but not in the presence of the connecting segment 1 ( CS 1 ) peptide , suggesting that enhancement of AICD observed in CD4+ CD8+ thymocytes is mediated through VLA 5 . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Adhesion of Baf 3 cells to fibronectin was induced by IL 3 in a dose dependent manner via very late antigen 4 ( VLA 4 ; alpha4beta1 integrins ) and VLA 5 ( alpha5beta1 integrins ) activation . ^^^ Constitutively active H Ras transfected Baf 3 cells adhered to fibronectin without IL 3 stimulation through VLA 4 and VLA 5 , whereas dominant negative H Ras transfected Baf 3 cells showed significantly less adhesion induced by IL 3 compared with wild type and constitutively active H Ras transfected Baf 3 cells . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
We show here that drug sensitive 8226 human myeloma cells , demonstrated to express both VLA 4 ( alpha4beta1 ) and VLA 5 ( alpha5beta1 ) integrin fibronectin ( FN ) receptors , are relatively resistant to the apoptotic effects of doxorubicin and melphalan when pre adhered to FN and compared with cells grown in suspension . ^^^ Whether VLA 4 mediated FN adhesion offers a survival advantage over VLA 5 mediated adhesion remains to be determined . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
However , this cell line did not express classical FN receptors such as very late antigen ( VLA ) 4 and VLA 5 , as estimated by immunofluorescent staining . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
In this study , we reported that affinity of beta 1 integrin very late Ag 5 ( VLA 5 ) for fibronectin was rapidly increased in bone marrow derived mast cells by Ag cross linking of FcepsilonRI . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
We found that cross linking of CD 43 with monoclonal antibodies ( MoAbs ) enhanced integrin alpha4beta1 ( very late antigen [ VLA ] 4 ) and alpha 5 beta1 ( VLA 5 ) dependent adhesion of human cord blood CD 34 ( + ) cells to fibronectin . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The absence of CD 45 correlated with enhanced adhesion to fibronectin via integrin alpha5beta1 ( VLA 5 ) , but not alpha4beta1 ( VLA 4 ) in both cell lines . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Taken together , our results suggest that FN and its receptors VLA 4 and VLA 5 might be involved in the entrance , migration and retention of inflammatory cells into the CNS during parasitic infections . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Flow cytometry indicates that cell surface expression of very late antigen 4 ( VLA 4 ) , VLA 5 , and the vitronectin receptor ( VNR ) remain constant following IFN gamma treatment , indicating the inhibitory effect of IFN gamma on adhesion to FN is not achieved through a reduction in integrin receptors for FN . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Fibronectin fragments modulate monocyte VLA 5 expression and monocyte migration . ^^^ To identify the mechanisms that cause monocyte localization in infarcted myocardium , we studied the impact of ischemia reperfusion injury on the surface expression and function of the monocyte fibronectin ( FN ) receptor VLA 5 ( alpha ( 5 ) beta ( 1 ) integrin , CD49e / CD29 ) . ^^^ Incubating monocytes with postreperfusion cardiac lymph that contained these FN fragments selectively reduced expression of VLA 5 , an effect suppressed by specific immunoadsorption of the fragments . ^^^ Treating monocytes with purified , 120 kDa cell binding FN fragments ( FN 120 ) likewise decreased VLA 5 expression , and did so by inducing a serine proteinase dependent proteolysis of this beta ( 1 ) integrin . ^^^ We postulated that changes in VLA 5 expression , which were induced by interactions with cell binding FN fragments , may alter monocyte migration into tissue FN , a prominent component of the cardiac extracellular matrix . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
To investigate the possible roles of the Ras / Rho family members in the inside out signals to activate integrins , we examined the ability of Ras / Rho small GTPases to stimulate avidity of alpha ( 5 ) beta ( 1 ) ( VLA 5 ) to fibronectin in bone marrow derived mast cells . ^^^ We found that both Ha Ras ( Val 12 ) and R Ras ( Val 38 ) had strong stimulatory effects on adhesion and ligand binding activity of VLA 5 to fibronectin . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The content of integrin alpha5beta1 ( VLA 5 ) , receptor for fibronectin , was increased at both lower and higher ion doses ( +22 and +57 % ) . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Interleukin 11 enhancement of VLA 5 mediated adhesion of CD34+ cells from cord blood to fibronectin is associated with the PI 3 kinase pathway . ^^^ In this report , we demonstrate that IL 11 enhanced adhesion of freshly isolated and 3 day expanded CD34+ cells to immobilized fibronectin . the expression of very late antigen ( VLA ) 4 and VLA 5 integrins was detected on CD34+ cells . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The more potent compounds were highly selective and did not affect U 937 cell adhesion to fibronectin ( VLA 5 ) , PMA differentiated U 937 cell adhesion to intercellular cell adhesion molecule 1 expressing Chinese hamster ovary cells ( LFA 1 ) and ADP induced platelet aggregation ( GPIIb / IIIa ) . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The more potent compounds were highly selective and did not affect U 937 cell adhesion to fibronectin ( VLA 5 ) , phorbolmyristate acetate or PMA differentiated U 937 cell adhesion to intercellular cell adhesion molecule 1 ( ICAM 1 ) expressing Chinese hamster ovary cells ( LFA 1 ) and adenosine diphosphate ( ADP ) induced platelet aggregation ( GPIIb / IIIa ) . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
We have previously reported that the priming of thioglycollate elicited peritoneal macrophages ( PMphi ) , as a representative population of mononuclear phagocytes ( MNP ) , by macrophage colony stimulating factor ( M CSF or CSF 1 ) rendered these cells more susceptible to secondary stimulation by extracellular matrix ( ECM ) proteins , in particular fibronectin ( FN ) , and that at least two beta 1 integrins , VLA 4 ( alpha4beta1 or CD49d ) and VLA 5 ( alpha5beta1 or CD49e ) , regulate IL 6 gene expression when PMphi come into contact with FN . ^^^ By using granulocyte macrophage colony stimulating factor ( GM CSF ) and IL 6 knockout ( null ) mice , we demonstrated that the cooperative effect between CSF 1 and FN in IL 6 release was a result of a sequential stimulation of the GM CSF , but not the TNF alpha , gene via interaction with VLA 5 . ^^^ The observed effects were specific because CSF 1 enhanced VLA 5 expression and blocking FN treated resident PMphi in vitro with VLA 5 monoclonal antibodies inhibited the IL 6 response . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
In this report , we show that VLA 5 mediated adhesion of bone marrow derived mast cells to fibronectin can be induced by two different pathways : first , FcepsilonRI clustering , which depends on calmodulin activation and extracellular Ca ( 2+ ) , and , second , by Mn ( 2+ ) stimulation , which is independent of calmodulin activation and antagonized by Ca ( 2+ ) . ^^^ We conclude that CALP 1 and 2 can inhibit VLA 5 mediated adhesion of mast cells to fibronectin through binding to EF hands of multiple proteins , and that these peptides can be used as lead compounds for the development of future therapy against allergy . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
A transduction strategy has been developed , using fibronectin ( FN ) assisted retroviral mediated gene transfer , based on the observation that hematopoietic stem and progenitor cells bind to specific adhesion domains of fibronectin , via the integrins , very late antigen 4 ( VLA 4 ) alpha 4 beta 1 and very late antigen 5 ( VLA 5 ) alpha 5 beta 1 . ^^^ Retrovirus mediated transduction on a recombinant FN fragment , FN CH 296 , containing binding sites for VLA 4 and VLA 5 , separated by type 3 repeats 12 to 14 , makes it possible to efficiently target hematopoietic stem and progenitor cells and T lymphocytes due to colocalization of target cells and retrovirus particles . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Using the highly invasive melanoma cell line BLM , we demonstrate that the chemokine Mig , a ligand for CXCR 3 , activates the small GTPases RhoA and Rac 1 , induces a reorganization of the actin cytoskeleton , and triggers cell chemotaxis and modulation of integrin VLA 5 and VLA 4 dependent cell adhesion to fibronectin . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
We therefore examined the efficacy of porcine very late antigen 5 ( VLA 5 ) and VLA 4 interactions with the human extracellular matrix ( ECM ) protein , fibronectin . ^^^ Peripheral blood mononuclear cells ( PBMCs ) from humans and miniature swine were flourochrome labeled and adhesion to plates coated with whole human fibronectin ( whFN ) or its 120 KDa fragment containing the VLA 5 binding region was determined . ^^^ Human and pig PBMC adhesion to human fibronectin ( hFN ) or 120 kDa fragment coated plates was assessed following incubation with control ab , anti VLA 4 , anti VLA 5 , or soluble fibronectin . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Biochemical engineering of the acyl side chain of neuraminic acids activates beta ( 1 ) integrins ( VLA 4 or VLA 5 ) , resulting in an increased adhesion of HL 60 cells to fibronectin . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
METHODS AND RESULTS : We observed that in all cases , ECM ligands and receptors ( such as fibronectin , laminin , VLA 5 and VLA 6 ) are enhanced in vitro , as ascertained by immunocytochemistry , ELISA and cytofluorometry . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Blocking antibodies to fibronectin , as well as to the integrins very late antigen 4 ( VLA 4 ) and VLA 5 , reduced monocyte adhesion to mesangial matrix by approximately 50 % . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
In particular , the expression of two important integrins , VLA 2 , the receptor for collagen , and VLA 5 , the receptor for fibronectin , as well as CD 44 , were examined in both cell lines after these had been exposed for 7 and 14 days to a 50 Hz , 0 . 5 mT field . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
In contrast , there were no remarkable differences between PB CD34+ cells before and after the 37 degrees C incubation in their expression of selectin ligand , VLA 4 , and VLA 5 or in their affinity for VCAM 1 or fibronectin . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The change of intracellular calcium ( [ Ca2+ ] 1 ) was determined by flow cytometry when human sperms were exposed to Fibronectin ( Fn ) as the specific ligand of integrin VLA 5 . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Concomitantly , membrane expression of fibronectin and laminin receptors ( VLA 4 , VLA 5 and VLA 6 ) was also enhanced on thymocyte subsets of infected mice . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
IDV appeared to act upon VLA 4 and VLA 5 since addition of antagonist monoclonal antibodies ( mAb ) similarly reduced adhesion of non treated DC to fibronectin . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
CD 81 facilitates both VLA 4 mediated leukocyte rolling and arrest on VCAM 1 under shear flow as well as VLA 5 dependent adhesion to fibronectin during short stationary contacts . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The aim of our study was to examine the co stimulatory effects of ECM components ( type 1 and 4 collagen , laminin , fibronectin ) in patients with SSc and to examine the expression of beta 1 integrins ( VLA 2 / CD49b , VLA 3 / CD49c and VLA 5 / CD49e ) on PBMC in these patients . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
We first showed , by immunocytochemistry , cytofluorometry , and RT PCR , that PTR produce ECM components , including fibronectin and laminin , and express the corresponding integrin type receptors , VLA 4 , VLA 5 , and VLA 6 . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The integrins VLA 2 ( alpha2beta1 CD49b / CD29 ) , receptor for laminin and collagen , VLA 5 ( alpha5beta1 CD49e / CD29 ) , receptor for fibronectin , VLA 6 ( alpha6beta1 CD49f / CD29 ) , receptor for laminin , and alphaVbeta 3 CD51 / CD61 ( receptor for vitronectin ) were evaluated by flow cytometry . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Conversely , binding and motility via VLA 5 across Fn were stimulated . ^^^ Growth of ex vivo generated LTC IC became unresponsive to these 3 ligands but was suppressed through VLA 5 engagement by the cell binding domain of Fn . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
In vitro , NOD BMDC showed increased fibronectin adhesion and increased VLA 5 expression . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Anchorage on fibronectin via VLA 5 ( alpha5beta1 integrin ) protects rheumatoid synovial cells from Fas induced apoptosis . ^^^ RESULTS : VLA 5 ( alpha5beta1 integrin ) , a major integrin expressed on RA FLS , was required for the adhesion of RA FLS on Fn . ^^^ This protection by Fn was reversed by anti VLA 5 mAb . ^^^ CONCLUSION : Anchorage of RA FLS on matrix Fn via VLA 5 protects RA FLS from Fas induced apoptosis , and Fn abundantly present in rheumatoid synovium appears to afford RA FLS resistance against apoptosis induction in vivo . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Phosphatidylinositol mannoside from Mycobacterium tuberculosis binds alpha5beta1 integrin ( VLA 5 ) on CD4+ T cells and induces adhesion to fibronectin . ^^^ Alpha5beta1 ( VLA 5 ) is one of two major FN receptors on T cells . ^^^ Thus , PIM interacts directly with VLA 5 on CD4+ T lymphocytes , inducing activation of the integrin , and promoting adhesion to the ECM glycoprotein , FN . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The fibronectin receptor , alpha subunit ( Itga 5 ) maps to murine chromosome 15 , distal to D15Mit16 . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
In addition , a remarkable induction was detected in the genes for syndecan 4 ( SDC 4 ) and alpha 5 integrin ( ITGA 5 ) , the cell adhesion molecules involved in the enhanced adhesion of cancer cells to fibronectin . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
By a genetic screening procedure with zebrafish , we identified two genes , integrinalpha 5 ( itga 5 ) and fibronectin ( fn ) , required for these processes . ^^^ Both Fibronectin accumulation and the epithelialization are dependent on itga 5 , which is expressed in the most medial part of somites . ^^^ Furthermore , with an additional deficiency in ephrin B2a , the segmental defect in itga 5 or fn mutant embryos is expanded posteriorly , indicating that both Integrin Fibronectin and Eph Ephrin systems function cooperatively in maintaining somite boundaries . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Basement membrane proteins , laminin , and collagen type 4 , as well as vitronectin and fibronectin , were identified in the intima and there was corresponding expression of integrin and non integrin receptors ( e . g . , CD 29 , CD49b , CD49d , CD49e , CD49f , CD 51 , CD 61 , CD 44 ) for these matrix proteins . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Analysis of the expression of adhesion receptors on the canine peripheral lymph DC revealed the presence of CD 54 , CD 58 , CD 18 as well as CD49d and CD49e molecules and cell surface fibronectin . ^^^ Antibody against fibronectin , a substrate for CD49d and CD49e receptors , reduced DC lymphocyte binding . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
In vitro examination was carried out to investigate the effects of protein A of Staphylococcus aureus ( S . aureus ) on the production of fibronectin ( Fn ) and the third component of complement ( C 3 ) by macrophages and that of CD11b , CD49e and H2O2 by granulocytes . ^^^ These results show that rPA and PA in CoCS are major components which stimulates Fn synthesis and secretion by cultured M phi some component ( s ) contained in the supernatant of overnight culture of S . aureus increased the production C 3 by cultured M phi , and the supernatant may not contain stimulators to induce production of CD49e , CD11b and H2O2 . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Significant differences in the expression of very late antigen ( VLA ) integrins , VLA alpha 4 chain ( CD49d ) , the classical fibronectin receptor VLA alpha 5 chain ( CD49e ) , leukocyte function associated molecule 3 ( LFA 3 ; CD 58 ) and fibronectin were observed between patients showing good ( > 10 % ) or poor ( < 10 % ) penetration in the sperm penetration assay ( P = 0 . 0068 ) . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Spermatozoa were incubated with antibodies against alpha 1 ( CD49a ) , alpha 2 ( CD49b ) , alpha 3 ( CD49c ) , alpha 4 ( CD49d ) , alpha 5 ( CD49e ) , alpha 6 ( CD49f ) chains of beta 1 integrins , beta 1 ( CD 29 ) , beta 2 ( CD 18 ) , alpha 5 ( CD 51 ) , beta 3 ( CD 61 ) and beta 4 integrin chains , the LFA 3 ( Lymphocyte function antigen , CD 58 ) from the immunoglobulin superfamily and the extra cellular matrix proteins laminin , fibronectin and collagen 4 . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
A monoclonal antibody ( mAb ) against CD49e blocked the adhesion of both types of moDC to FN . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
We have observed that in patients with high expression of laminin receptors CD49b , CD49f and lack of fibronectin receptors CD49d , CD49e more often renal failure has been confirmed . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Studies using blocking antibodies indicated the involvement of CD11a in binding to ICAMs , and of CD49d , CD49e , and CD 29 in binding to FN . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Therefore , we analyzed the expression of the FN receptors , VLA 4 ( CD49d ) and VLA 5 ( CD49e ) , by direct immunofluorescence , on these leukemic cells . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Adherent and nonadherent monocyte populations from TAID patients and healthy controls were subjected to a polarization test with the chemoattractant fMLP ( or MCP 1 ) , FACS analyses ( FITC labelled FN , CD 29 , CD49e , d , b and a ) and tested for their capability to develop into veiled APC . ^^^ Monocytes of healthy individuals up regulated CD49e and d integrins and their capability to bind FITC labelled FN after adherence to a FN coated plate , as well as enhancing their capability to generate T cell stimulatory VCs . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
The stimulation by IL 1alpha up regulated CD 44 , 49e , 51 , 54 , 61 , 106 on MC on polystyrene ; CD49e , 51 , 61 , 106 , 146 , 165 on MC on fibronectin , and CD49e , 51 , 54 on MC grown on human matrix . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
RESULTS : ( 1 ) The expression of CD49d , CD 44 , CD11a and CD49e on expanded CD 34 ( + ) cells increased or sustained the same levels as those of the fresh isolated UCB CD 34 ( + ) cells , while the expression of CD62L , CD 54 and CD 31 on expanded CD 34 ( + ) cells declined during the culture . ( 2 ) The spontaneous adhesion between CD 34 ( + ) and FN and SDF 1 induced adhesion continuously increased in the course of the first 10 day culture . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Monocytes of type 1 diabetic patients displayed an increased adhesion to fibronectin in comparison with type 2 patients and healthy control subjects but had a normal expression of the FN binding integrins CD 29 , CD49a , CD49d , and CD49e ( although CD11b and CD 18 expression was increased ) . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Confirmation of the assignment of the vitronectin ( VNRA ) and fibronectin ( FNRA ) receptor alpha subunits . ^^^ Using human rodent somatic cell hybrids and cDNA probes corresponding to the alpha subunits , we have confirmed the mapping of the fibronectin receptor ( FNRA ) to chromosome 12 and the vitronectin receptor ( VNRA ) to chromosome 2 . . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
Mapping of the alpha subunit of the fox fibronectin receptor ( FNRA ) to chromosome 8 . ^^^
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08648 and P02751	Pubmed	SVM Score :0.0
NA