| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :1.0282444 |
| Finally , TSP can interact with fibrinogen and fibronectin , perhaps on cellular surfaces , which might serve as secondary receptor like mechanisms for TSP binding and subsequent mediation of cell adhesion . . 1.0282444^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| Several cell growth and differentiation related genes , including thrombospondin 1 ( THBS 1 ) , SPARC [ secreted protein , acidic , cysteine rich ( osteonectin ) ] , and fibronectin ( FN 1 ) were also found to be upregulated in the nontumorigenic clones ; however , these were expressed in the slow growing tumorigenic clones as well . ^^^ Our results suggest that THY 1 is a putative tumor suppressor gene for ovarian cancer and that THBS 1 , SPARC , and FN 1 are genes associated with the regulation of in vivo tumor growth rate . . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| We also examined the roles of THBS 1 and CTGF in mediating high glucose and glycated albumin induced synthesis of the matrix protein , fibronectin , by mesangial cells . ^^^ In contrast to high glucose conditions , increases in fibronectin synthesis that were stimulated by glycated albumin were not dependent on THBS 1 activation of latent TGFB 1 . ^^^ In contrast to THBS 1 , CTGF is required for mesangial synthesis of fibronectin stimulated by high glucose or glycated albumin , and is thus a potential therapeutic target . . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| We have studied the distribution of fibronectin , laminin , heparan sulfate proteoglycan ( HSPG ) and collagen types 1 , 3 and 4 , along with the platelet alpha granule glycoprotein , thrombospondin ( TSP ) , by immunofluorescence in frozen sections of muscle from control denervating conditions and ALS patients . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| The GP IIb / IIIa complex is an adhesion receptor belonging to the integrin superfamily ; it can bind five adhesive proteins containing the arginine glycine aspartic acid ( RGD ) sequence in their structure : fibrinogen ( Fg ) , von Willebrand factor ( vWf ) , thrombospondin ( Tsp ) , fibronectin ( Fn ) and vitronectin ( Vn ) . ^^^ Fg mediates platelet aggregation ; vWf , Tsp and Fn are large molecules that support platelet adhesion to vessel wall ; Vn is a molecular connection among hemostasis and others physiological processes . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| Immunoreactivity to TSP in tissues was also compared with that of fibronectin , laminin , and collagen type 1 , 3 , and 4 . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| TSP immunoreactivity was compared to the pattern of von Willebrand factor , plasma transglutaminase ( blood coagulation factor 13 ) , fibronectin , and mononuclear phagocytes , using double label immunofluorescence microscopy . ^^^ The codistribution of catalytic factor 13 and two cross linking substrates , fibronectin and TSP , suggests a functional role of the enzyme in the extracellular matrix build up in PVR and PDR . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| Flow cytometry was used to detect platelet associated fibrinogen ( PA Fbg ) , platelet associated fibronectin ( PA FN ) and platelet associated thrombospondin ( PA TSP ) on the surface membrane of platelets and plasma ( P ) TSP in 30 patients with lung cancer ( 16 case of adenocarcinoma and 14 of squamous cell carcinoma ) . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| In the presence of chlorate , newly synthesized TSP is not incorporated into the cell matrix but mainly released into the culture medium , whereas localization and incorporation of newly synthesized fibronectin is not altered . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| Most of the active TGF beta released from platelets after degranulation was associated with TSP , as demonstrated by anti TSP immunoaffinity and gel permeation chromatography . 125I TGF beta binds to purified TSP in an interaction that is specific in the sense that bound TGF beta could be displaced by TGF depleted TSP but not significantly by native TSP , heparin , decorin , alpha 2 macroglobulin , fibronectin , or albumin . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| The cell attachment activity following TSP treatment was interfered with by the addition of a synthetic peptide containing the RGD sequence from the cell attachment region of fibronectin ( FN ) . ^^^ Cell attachment on TSP coated plates was inhibited by the addition of anti chicken FN rabbit antiserum , but not by anti chicken vitronectin ( VN ) rabbit antiserum . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| The kinetics of adhesion and spreading on TSP were similar to that of vitronectin ( VN ) , laminin ( LN ) , and fibronectin ( FN ) . ^^^ Activation of PMN with the calcium ionophore A 23187 or the chemotactic peptide FMLP increased PMN adherence to LN and FN , but not to TSP or VN , suggesting that PMN activation may differentially regulate expression of TSP and VN receptors as compared to LN and FN receptors . ^^^ Inasmuch as the 140 kDa fragment of TSP contains an Arg Gly Asp sequence similar to the cell recognition site of FN and VN , we determined whether RGDS peptides would inhibit PMN adhesion . ^^^ RGDS did not significantly inhibit PMN adhesion to TSP , VN , or LN , but reduced PMN adhesion to FN by 50 % . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| RNA slot blot analysis demonstrated that heparin treatment specifically decreased the steady state mRNA levels for both FN and TSP in the cells . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| Binding of both intact [ 125I ] EC TSP and of the 125I labeled 70 kDa fragment was inhibited by unlabeled TSP , heparin , fibronectin ( FN ) , monoclonal anti TSP antibody directed against the 70 kDa fragment ( B 7 3 ) , and by full serum , but not by heparin absorbed serum or the cell adhesion peptide GRGDS . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| We have shown that thrombospondin ( tsp ) , like fibronectin ( fn ) and von Willebrand factor ( vWf ) , exhibits a rapid , specific and saturable binding to collagen type 1 fibres ( from bovine tendon ) . ^^^ The conformation of tsp , like that of fn and vWf , is important since denaturation causes reduced complexing . ^^^ Tsp , like vWf but in contrast to fn , shows little affinity for denatured fibres emphasizing the importance of collagen conformation . ^^^ No evidence was found for a role for fn , the proteoglycan PG 2 or collagen type 5 , which could be present in type 1 fibres , in mediating the interaction between tsp and the fibres . ^^^ VWf did not inhibit the interaction of tsp , but fn did slightly when tested in large excess . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| Earlier studies from this laboratory have shown that infection of vascular cells with herpes simplex virus 1 or 2 ( HSV 1 , HSV 2 ) results in the differential suppression of extracellular matrix proteins including fibronectin ( FN ) , type 4 collagen , thrombospondin ( TSP ) and Factor 8 von Willebrand protein . ^^^ We have measured the steady state levels of mRNAs for several extracellular matrix proteins ( type 4 collagen , FN and TSP ) and two intracellular proteins ( actin and tubulin ) in human endothelial cells ( EC ) following HSV 1 infection . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| The mechanisms involved in erythroblast macrophage binding are still unknown , but the expression by macrophages and erythroid progenitors of receptors for fibronectin and thrombospondin ( TSP ) , as well as their respective ligands in the case of macrophages , suggests that these molecules could be involved in the formation of the erythroblastic island . . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| Chondrocytes expressed high levels of message for thrombospondin ( Tsp ) and fibronectin ( Fn ) . ^^^ RNA slot blot analysis demonstrated that treatment of the cultures with IL 1 ( 100 ng / ml ) for 24 h caused a 70 % suppression of their steady state Tsp mRNA levels whereas those of Fn were not affected . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| The adhesion promoting activity of TSP was species independent , specific , and not due to contamination by fibronectin , vitronectin , laminin , or platelet factor 4 . ^^^ The cell surface receptor for TSP is protein in nature and appears distinct from that for fibronectin . . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| Human epidermal keratinocytes obtained from normal skin attached and spread on thrombospondin ( TSP ) coated plastic dishes but failed to attach and spread on untreated plastic culture dishes or dishes coated with fibronectin or laminin . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| Migration of these cells as measured in two different assays ( migration out of an agarose drop explant , and into micropore filters in a modified Boyden chamber ) , is stimulated by fibronectin ( FN ) and to a lesser extent by thrombospondin ( TSP ) . ^^^ In contrast to these factors , interferon gamma ( INF gamma ) inhibits KC production of both FN and TSP and concomitantly inhibits both motility and proliferation . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| The keratinocytes were simultaneously examined for adhesiveness and production of two extracellular matrix molecules , e . g . , thrombospondin ( TSP ) and fibronectin ( FN ) . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| We have investigated the localization of thrombospondin ( TSP ) , fibrinogen , fibronectin , and von Willebrand factor in human platelets by transmission electron microscopy of antibody stained ultrathin frozen sections . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| We used flow cytometry to investigate the change of platelet membrane glycoproteins ( GPIb and GP IIb / IIIa ) and the distributions of fibrinogen ( Fbg ) , thrombospondin ( TSP ) and fibronectin ( Fn ) on the surface of thrombin stimulated platelets . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| High concentrations of exogenously added TSP as well as the recombinant form of the heparin binding domain from the TSP molecule also partially inhibited killing while laminin and fibronectin were ineffective . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| Incubation of cells in suspension with soluble TSP before or at the time of seeding onto fibronectin substrates resulted in an inhibition of focal adhesion formation . ^^^ Furthermore , the addition of TSP to fully adherent cells in situ or prespread on fibronectin substrates caused a reduction in the number of cells , which were positive for focal adhesions , although there was no significant effect on cell spreading . ^^^ The observed effects were specific for TSP and were not observed with platelet factor 4 , beta thromboglobulin , or fibronectin . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| The TSP values were then compared to the patients plasma levels of two traditional markers of platelet activation , beta TG and PF 4 , and to fibronectin ( FN ) and von Willebrand factor ( 8 : vWF ) . ^^^ No correlation was observed , in CRF patients , between the TSP level and PF 4 ( 2 . 5 + / 1 . 5 ng / ml ) , beta TG ( 131 . 1 + / 21 ng / ml ) , FVIII : vWF ( 252 + / 85 % ) , or FN ( 102 + / 33 % ) plasma levels . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| Using SDS PAGE we observed that synthesis of fibronectin ( FN ) , type 4 procollagen and thrombospondin ( TSP ) was inhibited in infected cultures as early as 2 hours becoming almost complete by 15 hours post infection . ^^^ The degree of inhibition of matrix protein synthesis was dependent on the dose of the virus inoculum ( MOI 20 greater than MOI 5 ) and varied with the particular matrix protein , i . e . shut off of type 4 collagen occurred first followed by that of FN and then TSP . ^^^ Suppression of matrix proteins in infected cultures was confirmed by measuring the level of FN and TSP in uninfected and infected cultures in an enzyme linked immunosorbent assay ( ELISA ) . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| The development of the TSP matrix was compared to that of fibronectin ( FN ) and of von Willebrand Factor ( VWF ) in the same cultures using our monoclonal anti TSP antibody B 7 3 and monospecific anti FN or anti VWF antibodies . ^^^ The first appearance of the fibrillar arrays of TSP coincides perfectly with that of the FN fibers ; extracellular VWF also was first detected at this point . ^^^ From this time in culture , there was an increasing coincidence of the TSP with the FN and the VWF matrices suggesting the formation of a complex multicomponent structure of the fibrillar network . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| In permeable resting cells , punctate staining similar to that reported for fibrinogen ( Fbg ) , thrombospondin ( TSP ) , fibronectin ( Fn ) , von Willebrand factor ( VWF ) , B thromboglobulin ( BTG ) , and platelet Factor 4 ( PF 4 ) was observed . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| Activation dependent adhesion of platelets to TSP substrates does not appear to be mediated solely by direct interaction of TSP with platelet membrane components , but appears to be mediated in large part by intervening bridging molecules , such as fibronectin or fibrinogen . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| Thrombospondin ( TSP ) , a multifunctional alpha granule glycoprotein of human platelets binds fibrinogen , fibronectin , heparin , histidine rich glycoprotein ( HRGP ) , and plasminogen ( Plg ) , and thus , may play an important role in regulating thrombotic influences at vessel surfaces . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| The distribution and transport of thrombospondin ( TSP ) , fibrinogen ( Fbg ) , fibronectin ( Fn ) , and Factor 8 related antigen ( 8 : RAg ) in resting and thrombin stimulated platelets was investigated by immunofluorescence microscopy . ^^^ Double label immunofluorescence staining for Fbg and either beta thromboglobulin ( beta TG ) , TSP , or Fn demonstrated co localization , indicating their presence in the same intracellular structures . 8 : RAg showed general co localization ; however , the staining was finer , suggesting a possible differential intragranular localization . ^^^ Thus , TSP , Fbg , Fn , and beta TG are localized in the same structure in resting cells . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| Thrombospondin ( TSP ) , a multifunctional alpha granule glycoprotein of platelets , binds fibrinogen , fibronectin , heparin , and histidine rich glycoprotein and thus may play an important role in regulating thrombotic influences at vessel surfaces . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| In bone matrix there are multiple glycoproteins that contain the integrin binding RGD sequence : fibronectin ( FN ) , thrombospondin ( TSP ) , osteopontin ( OPN ) , bone sialoprotein ( BSP ) , type 1 collagen ( COLL 1 ) , and vitronectin ( VN ) . ^^^ In this study , the localization of TSP , FN , VN , and several integrins within developing human long bone using immunohistochemical methods was examined , as was the effect of all bone RGD proteins on the adhesion of human osteoblastic cells . ^^^ COLL 1 , TSP , VN , FN , OPN , and BSP promoted bone cell attachment in a dose dependent manner and were equivalent in action when used in equimolar concentrations . ^^^ In the presence of GRGDS peptide in the medium , the adhesion to BSP , OPN , and VN was almost completely blocked ( 10 , 10 , and 15 % of control , respectively ) , and attachment to FN , COLL 1 , and TSP was only slightly decreased ( 80 , 75 , and 55 % , respectively ) . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| Dentine slices were soaked in TSP 1 , FN or buffer before carrying out resorption assays using bone cell suspensions prepared from the long bones of 18 20 day old pre hatch chick embryos . ^^^ Soaking dentine slices in FN resulted in a five fold increase in resorption over controls , while TSP 1 gave a two fold increase . ^^^ However , whereas the increase in resorption on FN soaked slices could be explained by a vast increase in cell adhesion to the slices , TSP 1 soaked wafers showed no increase in cell adhesion . ^^^ In confirmation of this , TSP 1 was able to further increase resorption on FN soaked slices where cell adhesion was already maximal . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| We examined the effects of proinflammatory cytokines on the expression of two extracellular matrix proteins , e . g . , thrombospondin ( TSP ) and fibronectin ( FN ) b cultured human umbilical vein endothelial cells ( HUVECs ) . ^^^ Treatment of HUVECs with human recombinant interleukin 1 beta ( IL 1 beta ) or human tumor necrosis factor alpha ( TNF alpha ) caused a time and dose dependent decline in TSP production whereas FN production was not modified . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| Localization of fibronectin ( Fn ) , thrombospondin ( Tsp ) , and F actin was examined in ( 1 ) primary rat tracheal epithelial ( RTE ) cells ; ( 2 ) spontaneously immortalized nonneoplastic cells ( SPOC 1 ) ; and ( 3 ) neoplastic cells ( EGV5T ) derived from tumors arising following transplantation of an N methyl N ' nitro N nitrosoguanidine transformed RTE cell line into nude mice . ^^^ Primary RTE cells display organized F actin stress fibers , perinuclear Fn and Tsp , and pericellular Fn in fibrillar arrays . ^^^ Fn and Tsp are localized to the perinuclear region with Fn staining more intensely . ^^^ Perinuclear staining of Fn and Tsp occurs in some cells within the colony . ^^^ Levels of Tsp RNA and Fn RNA and protein are significantly reduced in both cell lines compared to primary RTE cells . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| On GCT cells we considered cytoskeletal organization , adhesion properties , and integrin expression upon plating in serum free medium onto fibronectin ( FN ) , collagen ( COL ) , thrombospondin ( TSP ) , bone sialoprotein ( BSPII ) , and osteopontin ( OPN ) . ^^^ GCT cells promptly adhered and spread on FN , BSPII , and OPN , while only 50 % adhered on COL and none on TSP . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| TSP promoted attachment but not spreading of MDA MB 231 cells which attached and spread on FN and LN substrates . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| Anti CBP IgG and anti CSVTCG peptide IgG inhibited A 549 cell spreading and adhesion on TSP but not on fibronectin and laminin . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| Both osteoblastic ( ROS 17 / 2 . 8 , RCT 3 ) and nonosteoblastic ( ROS 25 / 1 , RCT 1 ) cell lines attached in a time and concentration dependent manner to plates coated with fibronectin ( FN ) , osteopontin ( OP ) , type 1 collagen ( Col 1 ) , type 4 collagen ( Col 4 ) , and laminin ( LN ) but only weakly to osteocalcin ( OC ) and thrombospondin ( TSP ) . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| On the other hand , the production of both fibronectin and type 4 collagen from mesangial cells was enhanced by TSP . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| Using mixed substrata containing different proportions of TSP 1 , and fibronectin , fascin microspike formation by H9c2 and C2C12 cells was found to be reduced on substrata containing 25 % fibronectin and abolished on substrata containing 75 % fibronectin . ^^^ Adhesion to intermediate mixtures of TSP 1 and fibronectin resulted in coassembly of fascin microspikes and focal contacts , colocalization of fascin with actin stress fiber bundles and altered distributions of beta 1 integrins , cortical alpha actinin , and tropomyosin . ^^^ In cells adherent on 50 % TSP 1 : 50 % fibronectin , GRGDSP peptide treatment decreased focal contact assembly and altered cytoskeletal organization but did not inhibit microspike assembly . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| Thereafter , the effects of TSP on the activation of transforming growth factor beta ( TGF beta ) and fibronectin production were investigated in MCs . ^^^ Exposure of MCs to TSP caused enhancement of fibronectin production in both media and cell layers in a TSP dose dependent manner with the maximum at a TSP concentration of 1 microg / ml . ^^^ The TSP induced increase in fibronectin production from MCs was completely prevented by concomitant treatment with 10 microg / ml anti TGF beta neutralizing antibody . ^^^ These results indicate that the TSP production is promoted by a high ambient glucose concentration in human MCs and that TSP , in turn , causes an increase in fibronectin production via activation of TGF beta . . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| Based on this assay , we found that apoptotic but not fresh PMN bound specifically to surface immobilized TSP ( 33 + / 0 . 03 10 10 ( 3 ) cells / well ) compared to fibrinogen , fibronectin or laminin ( 8 . 0 + / 0 . 3 10 10 ( 3 ) cells / well ) . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| The normal tubular basement membrane ( TBM ) contains laminin ( LM ) , collagen 4 ( containing collagen 4 alpha chain 1 [ COL4A1 ] and COL4A2 ) , thrombospondin ( TSP ) , and fibronectin ( FN ) . ^^^ Only TSP and FN extend beyond the TBM into the interstitial space . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| The degradation of extracellular matrix ( ECM ) adhesive glycoproteins , fibronectin ( FN ) , thrombospondin ( TSP ) and von Willebrand factor ( vWF ) , by human leukocyte cathepsin G and elastase , and by plasmin or thrombin , was analysed by immunoblotting after incubation of physiologic doses of the proteases with confluent human umbilical vein endothelial cells . ^^^ Elastase induced an almost complete disappearance of intact FN , TSP , and vWF from the ECM at 0 . 02 units / ml within 5 minutes of incubation at 37 degrees C . ^^^ TSP was extremely sensitive to degradation by all enzymes except thrombin , whereas vWF released from the ECM was more resistant to proteolysis than constitutively secreted vWF , and FN was poorly degraded by plasmin . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| Our objective was to elucidate the mechanism of adherence to activated EC monolayers and to determine whether the matrix proteins thrombospondin ( TSP ) and fibronectin ( FN ) are mediators of this adherence . ^^^ Using static adherence assays we investigated whether TSP , FN , or the integrins alpha ( 5 ) beta ( 3 ) and alpha ( 5 ) beta ( 1 ) mediated adherence . ^^^ Neither TSP nor FN was exposed in confluent cultures but they both became available as receptors after EC retraction . ^^^ These data suggest that SS RBC adhere to a complex of matrix TSP and FN maintained in an adhesive conformation by interactions with both integrins . . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| As extracellular matrix ( ECM ) is critical to MVEC survival , and cytokines which regulate ECM , such as transforming growth factor ( TGF ) beta 1 , have been reported anecdotally to be altered in TTP / HUS , we examined the role of TGF beta 1 and two ECM proteins , fibronectin and thrombospondin ( TSP ) , in these disorders . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| We assessed plasma concentrations of fibronectin ( FN ) and thrombospondin ( TSP ) during acute attacks and attack free periods of patients with familial Mediterranean fever ( FMF ) . ^^^ Plasma levels of FN and TSP were assayed by enzyme linked immunosorbent assay ( ELISA ) . ^^^ Both FN and TSP concentrations were found to increase during acute attacks . ^^^ Significant correlations were found between FN and TSP levels and the concentrations of acute phase response indicators ( P < 0 . 05 ) . ^^^ This study disclosed for the first time significantly higher increments in the plasma levels of FN and TSP during acute FMF attacks than in attack free periods . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| Expression of thrombospondin 1 ( TSP 1 ) and fibronectin ( FN ) in HTM cells was evaluated by Western blot analysis . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| Furthermore , contraction of these activated endothelial cells leads to exposure of extracellular matrix proteins , such as TSP , laminin , and fibronectin and their participation in adhesive interactions with bridging molecules from the plasma such as von Willebrand factor ( vWf ) released from endothelial cells , ultimately culminating in vasoocclusion and local tissue ischemia , the pathognomonic basis of vasoocclusive crisis . . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| These studies therefore identify the alpha4beta1 integrin on SS RBCs as a CD 47 activated receptor for TSP , VCAM 1 , and plasma fibronectin , revealing novel binding characteristics of this integrin . . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| Quantitative differences of immunofluorescence labelling for chondroitin sulfate proteoglycan ( CSPG ) , fibronectin ( FN ) , tenascin C ( TN C ) , and thrombospondin ( TSP ) were evaluated in intact rat dorsal and ventral roots and dorsal and ventral roots 2 and 4 weeks after rhizotomy using image analysis . ^^^ Four weeks after rhizotomy , the immunoreactivity for CSPG , TN C and TSP decreased in dorsal and increased in ventral root stumps , although a higher level of immunofluorescence for FN remained in both dorsal and ventral root stumps 4 weeks after injury in comparison to 2 weeks after injury . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| Expression of alphaB crystallin , fibronectin ( FN ) , plasminogen activator inhibitor ( PAI ) 1 , thrombospondin ( TSP ) 1 , and connective tissue growth factor ( CTGF ) mRNA and protein in medium perfused and TGF beta 2 perfused anterior segments was examined by Northern and Western blot analyses . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| In addition , the correlation of stromal TSP 1 expression with extracellular matrix components fibronectin and tenascin indicate its possible implication in tumour stroma remodeling . . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| We have demonstrated that cEC of different morphology display variations in the expression of fibronectin ( FN ) , thrombospondin 1 ( TSP 1 ) and collagen 4 ( C 4 ) . ^^^ Astrocytes were shown to contain FN , TSP 1 , TN and SPARC mRNA . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| Whereas cells adherent on fibronectin organize actin stress fibers , cells adherent on TSP 1 display prominent membrane ruffles and lamellae that contain radial actin microspikes . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| The highest SF binding capacities were observed for thrombospondin 1 ( TSP 1 ) , fibronectin ( Fn ) , and heparan sulfate proteoglycan , although SF did not bind to albumin . ^^^ Mature two chain SF and precursor single chain SF bound approximately equally well to TSP 1 and Fn . ^^^ Moreover , two SF alpha chain peptides ( NK 1 and NK 2 ) both bound to TSP 1 and Fn , suggesting that the whole SF molecule is not required for binding . ^^^ Based on binding competition assays , TSP 1 exhibited higher affinity for SF than did nine other ECM molecules , including Fn and heparan sulfate proteoglycan . ^^^ SF binding was modulated by binding interactions among ECM molecules ( TSP 1 Fn , TSP 1 collagen 1 , and Fn collagen 1 ) , suggesting that the matrix capacity to bind SF depends upon its exact composition . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| The major adhesive proteins postulated to play a role in tumor arrest in the vascular bed are thrombospondin 1 ( TSP 1 ) , laminin , fibronectin and hyaluronan proteoglycans . ^^^ The major tumor and vascular receptors mediating these adhesive interactions are the CSVTCG specific TSP 1 receptor , the 67 kD laminin receptor , the alpha 5 beta 3 vitronectin / TSP 1 / fibronectin receptor , and CD 44 hyaluronan receptor . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| Growth of the cells on a fibronectin substrate also suppressed induction of apoptosis by TSP 1 or the peptides . ^^^ These results demonstrate that induction of apoptosis by the TSP 1 analogues is not a general cytotoxic effect and is conditional on a lack of strong survival promoting signals , such as those provided by a fibronectin matrix . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| Immunoblot analysis of fibrinogen ( Fg ) , thrombospondin 1 ( TSP 1 ) , fibronectin ( Fn ) and von Willebrand factor ( vWf ) was performed on extracts of platelets exposed under stirring to increasing concentrations of plasmin for up to 10 min at 37 degrees C . ^^^ Under conditions leading to formation of large aggregates , Fg , Fn and TSP 1 are extensively degraded concomitantly with their secretion , and readily lost from the surface of aggregated cells . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| The central region of TSP 1 , when used as a bait in two hybrid system , showed strong binding to the fibronectin ( FN ) type 3 like repeats 4 7 of type 7 collagen NC 1 domain . ^^^ |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| Thrombospondin 1 ( TSP 1 ) and an alternatively spliced fibronectin ( Fn ) EIIIA isoform are adhesive proteins associated with embryogenesis and tissue remodeling . ^^^ We compared , by immunohistochemistry and in situ hybridization , the course of TSP 1 and Fn EIIIA expression in a model of glomerulonephritis induced by Habu snake venom ( HV ) and characterized by mesangial cell migration , proliferation , and extracellular matrix ( ECM ) synthesis . ^^^ At 24 hr after HV , TSP 1 and Fn EIIIA proteins localized in the central aspects of lesions associated with platelets and macrophages and at the margins of lesions coinciding with mesangial cell migration ( determined by Thy 1 staining ) . ^^^ Mesangial cells at this time expressed TSP 1 but not Fn EIIIA mRNA . ^^^ Therefore , platelet and macrophage derived Fn EIIIA and TSP 1 in early lesions are associated with mesangial cell migration . ^^^ |
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| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| Thrombospondin 1 ( TSP 1 ) interacts specifically with heparin and fibronectin in vitro and colocalizes with fibronectin and heparan sulfate in the extracellular matrix ( ECM ) . ^^^ We have previously shown that both heparin and fibronectin have two binding sites on the TSP 1 subunit which may require conformational change for their occupancy ( R . ^^^ To investigate the effect of TSP 1 binding to fibronectin and heparin on its functional conformation , TSP 1 was subjected to proteolysis in the presence and absence of ligands and of Ca2+ . ^^^ We found that while trypsin cleavage of free TSP 1 resulted in the inactivation of ligand binding , TSP 1 bound to either fibronectin or heparin remained stably associated with these ligands . ^^^ Cleavage by thrombin or tissue plasminogen activator ( tPA ) showed that Ca2+ depleted TSP 1 , when bound to fibronectin or to heparin , yielded proteolytic cleavage patterns typical of the Ca2+ containing form . ^^^ |
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| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| RPE cells grown on TSP 1 coated plates showed increased VEGF and FGF 2 in their media compared to cells grown on plates coated with type 4 collagen , laminin , vitronectin , or fibronectin . ^^^ |
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| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| The soluble peptide inhibited adhesion of cells to the immobilized TSP 1 peptide or spreading on intact TSP 1 but at the same concentrations did not inhibit attachment or spreading on type 4 collagen or fibronectin . ^^^ |
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| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| The ECM proteins , thrombospondin 1 ( TSP 1 ) , fibronectin ( Fn ) , and vitronectin ( Vn ) can induce VSMC migration ; however , the cellular signaling pathways involved are not identical for each ECM protein . ^^^ We sought to elucidate the roles of these enzymes in TSP 1 , Fn , and Vn stimulated VSMC migration . ^^^ TSP 1 , Fn , or Vn ( 20 microg / mL ) or serum free media ( SFM ) was placed in the bottom wells of the chamber . ^^^ RESULTS : The VSMC migration was significantly increased by TSP 1 , Fn , and Vn . ^^^ LY 294002 inhibited TSP 1 , Fn , and Vn stimulated VSMC migration ( 85 % to 89 % , P < . 05 ) . ^^^ |
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| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| Shear loading of monospecific anti fascin immunoglobulins , which block the binding of fascin to actin into cytoplasm , strongly inhibited spreading , actin cytoskeletal organization and migration on TSP 1 and also affected the motility of cells on fibronectin . ^^^ |
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| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| This conformation was not induced by known cell surface TSP 1 receptors , whereas binding of F 18 was stimulated when TSP 1 bound to fibronectin but not to heparin or fibrinogen . ^^^ Conversely , binding of F 18 to TSP 1 enhanced TSP 1 binding to fibronectin . ^^^ Exogenous fibronectin also stimulated TSP 1 dependent binding of F 18 to melanoma cells . ^^^ Binding of the fibronectin TSP 1 complex to melanoma cells was mediated by alpha4beta1 and alpha5beta1 integrins . ^^^ Furthermore , binding to F 18 or fibronectin strongly enhanced the adhesive activity of immobilized TSP 1 for some cell types . ^^^ |
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| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| In the present study , we investigated the effect of the angiotensin AT ( 1 ) and AT ( 2 ) receptors on DNA synthesis as well as on the expression of the extracellular matrix ( ECM ) components , thrombospondin 1 ( TSP 1 ) and fibronectin ( FN ) in endothelial cells ( EC ) . ^^^ |
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| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| T lymphocyte adhesion to fibronectin and collagen type 4 induces TSP 1 expression on the cell surface via a brefeldin sensitive mechanism . ^^^ |
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| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| Using single and double immunostaining techniques , renal tissues were examined for TSP 1 protein , TGF beta 1 , platelet derived growth factor BB , extracellular matrix proteins , such as collagens and fibronectin , myofibroblast formation and macrophage influx . ^^^ |
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| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| HUVECs adhered more weakly on TSP 1 coated surfaces , remaining round shaped , as compared to the well spread phenotype displayed on fibronectin and gelatin . ^^^ |
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| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| Relative to C2C12 cells , BC3H1 cells underaccumulated cyclin dependent kinase inhibitor p 21 and underaccumulated transcripts for p 21 , GADD 45 , CDO , decorin , osteopontin , H 19 , fibronectin , and thrombospondin 1 ( tsp 1 ) . ^^^ Levels of accumulation of H 19 , tsp 1 , and larger isoforms of fibronectin messenger ribonucleic acid ( mRNA ) were found to increase in response to expression of myogenic regulatory factors as shown by their accumulation in differentiated myogenically converted 10T1 / 2 cells but not in 10T1 / 2 fibroblasts . ^^^ Our findings suggest participation of tsp 1 and specific isoforms of fibronectin in myogenesis and suggest additional avenues of research in myogenesis and oncogenesis . . ^^^ |
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| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| When rat mesangial cells were cultured with an amino acid mixture designed to replicate the composition in plasma after protein feeding , production of mRNA ( Northern blot analysis ) and / or protein ( ELISA or Western blot analysis ) for transforming growth factor beta 1 ( TGF beta 1 ) , fibronectin , thrombospondin 1 ( TSP 1 ) , and collagen 4 were enhanced in a manner comparable to a culture with high glucose ( 30 . 5 mM ) . ^^^ The TSP 1 antagonist LSKL peptide reduced bioactive TGF beta and fibronectin , indicating the dependence of TGF beta 1 activation on TSP 1 . ^^^ |
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| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| With a binding assay , TSP 1 interacted physically with MMP 2 , and TSP 1 bound MMP 2 showed the strongest binding activity in comparison with collagen 1 , fibronectin , and elastin . ^^^ |
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| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| We investigated the response of human skin fibroblasts to elevated glucose level ( 11 . 0 and 30 . 0 mM ) in terms of : ( 1 ) the expression and secretion of fibronectin ( FN ) and plasminogen activator inhibitor 1 ( PAI 1 ) ; ( 2 ) the accumulation of hyaluronic acid ( HA ) in pericellular matrix and in the conditioned medium ; ( 3 ) TGFbeta 1 expression , secretion and activation ; ( 4 ) TSP 1 expression and secretion . ^^^ |
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| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| In double labeling experiments , staining for TSP 1 did not overlap with that of fibronectin or type 6 collagen . mRNA for TSP 1 was detected in both fresh and cultured TM cells . ^^^ |
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| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| PURPOSE : To study whether glaucomatous extracellular matrix ( ECM ) modifications in the lamina cribrosa might be induced by TGF beta 2 , the effect of TGF beta 2 on the expression of collagen types 1 ( Col 1 alpha 1 ) , 3 ( Col 3 alpha 1 ) , and 4 ( Col 4 alpha 2 ) ; fibronectin ( FN ) ; tissue transglutaminase ( TGM 2 ) ; connective tissue growth factor ( CTGF ) ; and thrombospondin ( TSP 1 ) in cultured human optic nerve head ( ONH ) astrocytes was investigated . ^^^ Expression of Col 1 alpha 1 , Col 3 alpha 1 , Col 4 alpha 2 , FN , TGM 2 , CTGF , and TSP 1 was examined by semiquantitative RT PCR and Northern and Western blot analyses . ^^^ RESULTS : TGF beta 2 treatment upregulated the expression of Col 1 alpha 1 , Col 4 alpha 2 , FN , CTGF , TGM 2 , and TSP 1 mRNA and protein in cultured astrocytes . ^^^ Transfection of 10 nM CTGF siRNA inhibited the TGF beta 2 induced upregulation of CTGF , Col 4 alpha 2 , Col 1 alpha 1 , TGM 2 , and FN , whereas TSP 1 expression was not reduced . ^^^ |
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| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| Furthermore , 2 genes , Fibronectin ( FN ) and Thrombospondin ( TSP 1 ) involved in cellular differentiation and the regulation of tumor angiogenesis , respectively , were found to be up regulated upon THY 1 induction . ^^^ |
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| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| The increased levels of fibronectin and active Akt / PKB were also observed in retinal vasculature of TSP 1 / mice in vivo . ^^^ Thus , our results demonstrate that in the absence of TSP 1 , the proangiogenic signaling is enhanced , possibly through up regulation of fibronectin expression . ^^^ |
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| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07996 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
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