| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :1.2757754 |
| By expressing in vitro a mutant PON 1 with a cleavable N terminus , we demonstrate that PON 1 associates with lipoproteins through its N terminus by binding phospholipids directly rather than binding apoA 1 . 1.2757754^^^ |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.5163578 |
| Further , the displacement of PON 1 by apoA 2 could explain in part why PON 1 is mostly found in HDL particles with apoA 1 and without apoA 2 , as well as the poor antiatherogenic properties of apoA 2 rich HDL . . 0.5163578^^^ |
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| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| Interstitial fluid PON 1 concentration was dependent on the interstitial fluid apo AI concentration ( r = 0 . 690 , P < 0 . 005 ) indicating PON 1 remained associated with HDL . ^^^ However , the ratio of PON 1 concentration to apo AI was lower in interstitial fluid ( 0 . 60 + / 0 . 20 ) than in the serum ( 0 . 95 + / 0 . 18 ) ( P < 0 . 001 ) indicating sequestration of PON 1 in the sub intimal space . ^^^ |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| Subjects from Belfast also had significantly higher serum cholesterol , triglycerides , LDL cholesterol , and apolipoprotein B , and significantly lower HDL cholesterol and apolipoprotein A 1 , but these lipoprotein parameters were independent of the PON 1 192 polymorphisms . ^^^ |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| Human serum paraoxonase ( PON 1 ) is closely associated with a specific HDL subfraction also containing apoA 1 and clusterin . ^^^ |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| Human paraoxonase ( PON 1 ) is a calcium dependent esterase closely associated with high density lipoprotein ( HDL ) containing apolipoprotein AI ( apoAI ) , which has been shown to confer antioxidant properties to HDL . ^^^ We have undertaken a study of the effect of the lipid lowering drug simvastatin on serum PON 1 activity ( in relation to paraoxon and arylesterase activity ) , on apoAI containing and apolipoprotein B ( apoB ) containing lipoproteins , and on lipid peroxide concentrations in 64 ( 39 women and 25 men ) unrelated FH patients . ^^^ We conclude that simvastatin may have important antioxidant properties through increasing serum PON 1 activity , perhaps as a consequence of reducing oxidative stress , by a mechanism independent of apoAI containing lipoprotein concentration and without the influence of PON 1 192 and PON 1 55 genetic polymorphisms . ^^^ |
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| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| Genotype information included measurements at 18 diallelic loci in six coronary heart disease candidate susceptibility gene regions : APOA 1 C 3 A 4 , APOB , APOE , LDLR , LPL , and PON 1 . ^^^ |
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| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| To characterize the nature of PON 1 in apoA 1 deficiency , we investigated PON 1 in an apoA 1 deficient patient . ^^^ When serum was analyzed on fast protein liquid chromatography , PON 1 protein was distributed almost exclusively on HDL despite the absence of apoA 1 ; on the other hand , 38 . 5 % of PON 1 protein was found in the lipoprotein free fraction when the lipoproteins were fractionated through ultracentrifugation . ^^^ Our results demonstrated that PON 1 protein possesses a preferential association with HDL even in the absence of apoA 1 , although apoA 1 is a crucial factor for the maximal activity and stabilization of PON1 . . ^^^ |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| Accumulation of PON 1 in the cell membrane was not influenced by the ability of the cell to co secrete of apoA 1 . ^^^ |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| Therefore , we subjected apolipoprotein A 1 proteoliposomes containing either 1 palmitoyl 2 linoleoyl sn glycero 3 phosphocholine or 1 palmitoyl 2 arachidonoyl sn glycero 3 phosphocholine to oxidation by a peroxynitrite generator , SIN 1 , in the presence and absence of purified PON 1 . ^^^ |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| Human paraoxonase ( PON 1 ) is a calcium dependent esterase exclusively bound to apolipoprotein A 1 and clusterin , containing high density lipoprotein ( HDL ) particles that hydrolyzes organophosphates and aryl esters . ^^^ |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| In hyperthyroid patients , significantly lower PON 1 activity ( 45 + / 23 vs 67 + / 37 IU / ml , p < 0 . 001 ) , triglycerides ( 112 + / 53 vs 166 + / 130 mg / dl , p < 0 . 05 ) , apolipoprotein A 1 ( 137 + / 26 vs 154 + / 21 mg / dl , p < 0 . 001 ) and apolipoprotein B ( 75 + / 18 vs 86 + / 25 mg / dl , p < 0 . 05 ) were found . ^^^ |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| A number of studies have examined the potential role in longevity of other genes involved in vascular risk , haemostasis , and blood pressure regulation [ methyltetrahydrofolatereductase ( MTHFR ) , apolipoprotein A 1 ( APOA 1 ) , apolipoprotein C 3 ( APOC 3 ) , apolipoprotein A 4 ( APOA 4 ) , paraoxonase 1 ( PON 1 ) , plasminogen activator inhibitor type 1 ( PAI 1 ) ] , with contrasting results . ^^^ |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| Profiles of PON 1 activity and ApoA 1 , ApoA 2 , ApoE , ApoJ , and cholesterol concentrations were obtained by use of various density gradients . ^^^ |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| Similarly , macrophage loading with LPC ( by either adding LPC , or PON 1 or phospholipase A ( 2 ) ) significantly increased apolipoprotein A 1 ( apoA 1 ) mediated cholesterol efflux by 104 , 65 and 56 % , respectively , in ABCA 1 overexpressing macrophages . ^^^ |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| Serum PON 1 activity towards both paraoxon and phenylacetate , but not diazoxon , was positively correlated with HDL cholesterol ( HDL C ) and apo AI concentrations . ^^^ |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| High affinity , stability , and lactonase activity of serum paraoxonase PON 1 anchored on HDL with ApoA 1 . ^^^ This study examined the interaction of recombinant PON 1 with reconstituted HDL comprised of PC , cholesterol , and various apolipoproteins ( apoA 1 , 2 , and 4 ) . ^^^ We found that PON 1 binds apoA 1 HDL with sub nanomolar affinities ( K ( d ) < < 10 ( ) ( 9 ) M ) and slow dissociation rates ( t ( 1 / 2 ) > 80 min ) , while binding affinity for other particles was dramatically lower . ^^^ A truncated form of PON 1 lacking the N terminal helix maintains considerable binding to apoA 1 HDL ( K ( d ) = 1 . 2 10 10 ( ) ( 7 ) M ) , validating the structural model which indicates additional parts of the enzyme involved in HDL binding . ^^^ Overall , the results indicate the high stability , selectivity , and catalytic proficiency of PON 1 when anchored onto apoA 1 HDL , toward lactone substrates , and lipophilic lactones in particular . . ^^^ |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| There was significantly reduced plasma paraoxonase ( PON 1 ) activity , impaired HDL vascular antiinflammatory function , and increased basal levels of monocyte chemotactic protein 1 in the plasma of LA apoA 1 / mice compared with LA apoA I+ / + mice . ^^^ |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| Paraoxonase 1 ( PON 1 ) associates to specific high density lipoproteins ( HDLs ) those containing apolipoprotein A 1 ( apoA 1 ) and apolipoprotein J ( apoJ ) and is largely responsible for their antiatherogenic properties . ^^^ Serum and liver paraoxonase and arylesterase activities , serum triglyceride , total and HDL cholesterol concentrations , serum PON 1 , apoA 1 and apoJ contents and liver PON 1 mRNA levels were measured . ^^^ Serum PON 1 content was higher in female rats and decreased in both genders with CR , whereas apoA 1 and apoJ contents , which were higher in female rats too , decreased only in the former animals , accounting for the high PON 1 activity decrease observed in these animals . ^^^ In conclusion , the short term CR associated reduction of serum PON 1 activity and PON 1 , apoA 1 and apoJ levels points toward a reduced stability of HDL PON 1 complexes and / or HDL particle levels responsible for PON 1 transport and function in the blood . ^^^ |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| Highly significant ( P < 0 . 0001 ) , positive associations were observed between PON 1 activities and concentrations and HDL cholesterol and apolipoprotein A 1 ( apoA 1 ) concentrations in cases and controls . ^^^ Corrected slopes were significantly different in cases ( cases vs . controls : arylesterase , r = 0 . 19 vs . 0 . 38 , P < 0 . 02 for apoA 1 and r = 0 . 15 vs . 0 . 34 , P < 0 . 02 for HDL cholesterol ) such that if PON 1 should influence serum HDL , it would be less effective in coronary cases . ^^^ When examined as a function of the PON 1 gene promoter polymorphism C 107 T , highly significant differences ( P < 0 . 001 ) in HDL cholesterol and apoA 1 were observed between genotypes for controls , with high expresser alleles having the highest HDL concentrations . ^^^ |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| High density lipoprotein ( HDL ) associated paraoxonase 1 ( PON 1 ) anti atherogenic properties in macrophages , i . e . inhibition of cell mediated oxidation of low density lipoprotein ( LDL ) and stimulation of cholesterol efflux , were studied using recombinant variants of PON 1 and apoA 1 expressed in Escherichia coli and reconstituted HDL ( rHDL ) particles composed of phosphatidylcholine / free cholesterol ( PC / FC ) and apoA 1 . ^^^ PON 1 lactonase activity is stimulated by apoA 1 by approximately 7 fold relative to PC / FC particles . ^^^ Wild type ( WT ) PON 1 bound to rHDL inhibited macrophage mediated LDL oxidation and stimulated cholesterol efflux from the cells to 2 . 3 and 3 . 2 fold greater extents , respectively , compared with WT PON 1 bound to PC / FC particles without apoA 1 . ^^^ |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| In support of this , the stimulatory role of apolipoprotein A 1 was less prominent for DMPC bound PON 1 than for DMPS bound PON 1 . ^^^ |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : During inflammation , the serum amyloid A ( SAA ) content of HDL increases , whereas apolipoprotein A 1 ( apoA 1 ) and paraoxonase 1 ( PON 1 ) decrease . ^^^ Because cytokines stimulate the hepatic expression of inflammatory markers , we investigated their role in regulating SAA , apoA 1 , and PON 1 expression . ^^^ METHODS AND RESULTS : A cytokine mixture ( tumor necrosis factor [ TNF ] alpha , interleukin [ IL ] 1beta , and IL 6 ) simultaneously induced SAA and repressed apoA 1 and PON 1 expression levels . ^^^ Consistent with these findings , the basal level of SAA was increased , whereas apoA 1 and PON 1 decreased in primary hepatocytes from PPARalpha deficient mice as compared with wild type mice . ^^^ Moreover , neither WY 14643 nor fenofibrate had any effect on SAA , apoA 1 , or PON 1 expression in the absence of PPARalpha . ^^^ |
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| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| Using a multivariate analysis , we found that ( 1 ) genotypes of APOB , PON , LPL , LDLR , and APOE were significantly associated with variation of plasma apo B related traits ; ( 2 ) genotypes of PON , LPL , and APOC 3 were significantly associated with variation in plasma triglycerides ; and ( 3 ) genotypes of VLDLR , APOC 3 , LDLR , and APOE were significantly associated with variation in plasma apo AI and HDL cholesterol . ^^^ |
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| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| With the exception of a marginal effect on apolipoprotein A 1 levels in Indians , no other significant association was observed between the PON polymorphism and quantitative lipid traits in either racial group . ^^^ |
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| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| In normal aortas ( n = 6 ) there were low levels of extracellular Pon , clusterin , and apoA 1 , immunoreactivity . ^^^ The cytoplasm of smooth muscle cells in the media showed granular positivity for both Pon and apoA 1 , indicating that these proteins were undergoing lysosomal degradation . ^^^ With the progression of disease from fatty streaks ( n = 3 ) to advanced atherosclerosis ( n = 8 ) there was an increase in Pon , apoA 1 , and clusterin immunoreactivity , indicating the increasing presence of these proteins with disease progression . ^^^ The increase in Pon , clusterin , and apoA 1 during the development of atherosclerosis may therefore represent a protective response to the oxidative stress associated with the development of atherosclerosis . . ^^^ |
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| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| Compared to Lcat ( + / + ) mice , HDL cholesterol is reduced 94 % and apoA 1 , 90 % , in Lcat ( / ) mice ; this reduction in HDL is paralleled by a 71 % decrease in PAF AH activity and in a 58 % decrease in PON activity . ^^^ Even though apolipoprotein A 1 ( apoA 1 ) concentration and PON activity were drastically reduced , there was no reduction in apoA 1 and PON liver mRNA levels suggesting that post transcriptional events are responsible for the reduction of plasma PON and apoA 1 levels . ^^^ Fast protein liquid chromatography ( FPLC ) revealed that in Lcat ( + / + ) mice both PON and PAF AH activity is associated with large , apoA 1 containing HDL particles ( 9 . 7 nm by non denaturing gradient gel electrophoresis ) while in Lcat ( / ) mice both enzymes are associated with small 8 . 2 nm particles . ^^^ We conclude that the concomitant reduction in HDL and apoA 1 concentrations and PON and PAF AH activities is best explained by rapid clearance of the small HDL particles found in LCAT deficiency . . ^^^ |
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| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| After mice consumed an atherogenic diet for 1 or 3 days , switching the mice to a low fat chow diet for 3 days resulted in a return to baseline levels of lipid hydroperoxides but only a small return toward baseline for HDL cholesterol , with no significant increase in apoA 1 mass or PON activity and mass . ^^^ Concomitant with these changes in apoA 1 levels , HDL cholesterol and PON activity and mass declined without changes in mRNA levels for apoA 1 or PON , suggesting increased clearance of these altered HDL particles . ^^^ |
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| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| Our purpose was to examine the effect of the PON genotype on HDL C and apolipoprotein AI ( apo AI ) responses to pravastatin treatment . ^^^ The PON genotype thus modifies the effect of pravastatin on serum HDL C and apo AI concentrations . ^^^ |
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| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| Genetic variations of paraoxonase ( PON ) correlate with HDL cholesterol and apolipoprotein A 1 ( apoA 1 ) , suggesting antiatherogenic properties . ^^^ In the absence of apoA 1 in mice , total PON AEase is reduced and > 60 % is lipid poor . ^^^ PON AEase level and distribution is restored in apoA 1 / mice injected with adenoviruses encoding human apoA 1 and in transgenic mice expressing human apoA 1 at a steady state level . ^^^ Thus , while apoA 1 is not required for the HDL association of PON AEase , induced variations in apoA 1 correlate with changes in HDL associated , but not lipid poor , PON AEase . ^^^ PON AEase associates only with apoA 1 or apoE containing HDL but not VLDL . ^^^ |
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| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| In the control group , the QQ genotype of PON gene was found to have higher serum apoA 1 levels , compared with the RR genotype ( P < 0 . 05 ) . ^^^ |
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| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| To assess whether the increased PON activity was due to elevated HDL and apoA 1 level , we standardized PON activity for HDL and apoA 1 concentrations . ^^^ The standardized values for HDL ( PON / HDL ) increased ( p < 0 . 05 ) while the PON / apoA 1 ratio did not change significantly . ^^^ |
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| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| HDL C , Apo AI , Apo B , concentrations and PON activity were measured before and after 2 and 4 months of treatment as well as 2 months after withdrawing the treatment . ^^^ RESULTS : After 4 months of treatment , an elevation in the serum levels of Apo B ( P < 0 . 0001 ) and a marked decrease in the concentration of HDL C ( P < 0 . 0001 ) , Apo AI ( P < 0 . 0001 ) and PON activity ( P < 0 . 0001 ) were found . ^^^ A significant correlation between PON and both Apo AI ( r=0 . 270 , P < 0 . 04 ) and HDL C ( r=0 . 455 , P < 0 . 0001 ) and also between HDL C and Apo AI ( r=0 . 305 , P < 0 . 02 ) were found . ^^^ It is possible that ND reduces PON activity mostly by reducing both the HDL C and Apo AI levels . . ^^^ |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27169 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|