| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| While the efflux Km with apoA 1 and A 2 were 7 . 5 and 4 . 5 micrograms / ml for MP , those for SMC and FB were both 1 microgram / ml or lower , as low as 1 / 1500 and 1 / 500 of their plasma concentrations , respectively . ^^^ |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| Kinetic analysis of one mutant apoA 1 form showed similar Vmax but a 15 fold increase in the Km of the mutant apoA 1 form . ^^^ |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| Apparent Km values of the cholesterol release were estimated as 0 . 11 , 0 . 14 , and 0 . 24 microM , and Vmax values 35 , 11 , and 14 micrograms of cholesterol / mg of cell protein / 6 h , for apoA 1 , A 2 , and E , respectively . ^^^ Because the Km values are as low as 1 : 340 400 , 1 : 140 160 , and 1 : 6 8 of plasma concentrations of apoA 1 , A 2 , and E , respectively , the results have physiological relevance for a function of the free apolipoproteins in interstitial fluid to form high density lipoprotein and to reduce cellular cholesterol . . ^^^ |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| In 26 male patients in a 100 km run , concentrations of the HDL constituents cholesterol , apolipoprotein A 1 , phosphatidyl choline and sphingomyelin were determined before and immediately after the run . ^^^ |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| In contrast , changes in the cholesterol content of native and recombinant HDL have no significant effect on the apparent Km values when expressed in terms of the concentration of either apoA 1 or palmitoyloleoyl phosphatidylcholine . ^^^ Increasing the cholesterol content of the different HDL particles progressively increases the particle net negative charge , and these changes in apoA 1 charge are strongly correlated with both the Vmax and apparent Km values for LCAT . ^^^ |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| Linear regression analysis of the kinetic data shows that the ratio app Vmax / app Km was significantly lower ( p < 0 . 01 ) for glycated apolipoprotein A 1 ( 0 . 29 nmol . l / h . mumol ) than for normal apolipoprotein A 1 ( 0 . 78 nmol . l / h . mumol ) . ^^^ |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| L alpha Dimyristoylphosphatidylcholine 10F6 apoA 1 complexes had a 5 6 fold lower apparent Vmax / apparent Km as compared with wild type apoA 1 containing particles . ^^^ |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| The apparent catalytic efficiency ( Vmax ( app ) / Km ( app ) ) of the apoA 1 mutants ranged from 17 . 8 to 107 . 2 % of the control and was the result of variations in both the Km and the Vmax in the different mutants . ^^^ |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| Finally , we showed that the reaction of the hybrid HDL with LCAT was inhibited 2 5 fold , relative to apoA 1 rHDL , due to a corresponding increase in the apparent Km value . ^^^ |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| Compared to normal apoA 1 , the LCAT cofactor activity of apoA 1 ( P165R ) and apoA 1 ( R160L ) Oslo as defined by the ratio of Vmax to appKm was reduced significantly by 62 % and 29 % , respectively ( here and throughout the text , the apparent Km is given as Michaelis Menten kinetics do not take particle binding into account and therefore would result in errors with an interfacial enzyme such as LCAT ; Vmax estimates are not affected by this error ) . ^^^ |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| Compared to normal apoA 1 , apoA 1 ( R151C ) Paris had a reduced LCAT cofactor activity with a 60 % lower Vmax / Km ratio due to a 50 % higher affinity constant , Km . ^^^ |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| Log Vmax correlated with log triglyceride ( beta = 0 . 307 ; P = 0 . 02 ) , and high density lipoprotein ( HDL ) ( beta = 0 . 74 ; P = 0 . 03 ) whilst Km correlated with HDL ( beta = 1 . 73 ; P < 0 . 001 ) and apoAI ( beta = 1 . 76 ; P = 0 . 0048 ) , LDL ( beta = 0 . 14 ; P = 0 . 05 ) , and creatine kinase ( beta = 0 . 003 ; P = 0 . 01 ) . ^^^ |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01834 and P02647 |
Pubmed |
SVM Score :0.0 |
| NA |
|