| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| The analyses of CK 5 ( the usual partner of CK 14 ) gene expression using specific cDNA and antibody clearly demonstrated its absence in LECs . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| More rarely , they reacted with antibodies recognizing CK 5 , CK 13 , and CK 14 , which consistently labeled the basal cells of lacrimal ducts . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| CK 5 and CK 14 were prominent markers of basal and basaloid keratinocytes , whereas a decrease in staining occurred in advanced maturation stages and areas of terminal keratinization . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| CK 5 and CK 14 were not detected in a majority of samples and CK 18 , a marker of simple epithelia , was aberrantly expressed in 18 samples . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| In addition , four chordoma specimens were focally positive for keratin 903 , which reacts with high molecular weight CKs such as CK 1 , CK 5 , CK 10 , and CK 14 ; one specimen also showed a strong CK 7 expression . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| METHODS : Eight cases of ASC of the liver were analyzed both clinicopathologically and immunohistochemically using antibodies of cytokeratin ( CK ) 7 , CK 8 , CK 18 , CK 19 , and CK 903 ( CK 5 , CK 10 , CK 11 , and CK 14 ) . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| CK 8 positive reactions were detected in the normal alveolar and ductal epithelia and CK 5 and CK 14 positive reactions were seen in myoepithelial cells of nonlactating mammary glands . ^^^ Positive staining for CK 5 and CK 8 was detected in all tumours and CK 14 was expressed in those with a papillary pattern . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| We have investigated the pathway of differentiation in human developing junctional epithelium using monoclonal antibodies and two dimensional gel electrophoresis of microdissected tissue to identify CK 19 , CK 16 , CK 14 , CK 13 , CK 6 , CK 5 , CK 4 in the junctional epithelium ( JE ) over partially erupted human teeth . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| Of 20 leucoplakia samples analysed , CK 5 was not detected in 17 samples , while CK 14 was not found in 13 samples . ^^^ Non expression of CK 5 was observed in five of seven submucous fibrosis samples , while CK 14 was not detected in only two samples . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| These results of one and two dimensional electrophoretic analysis suggest the presence of CK 5 , CK 7 , CK 8 , CK 13 , CK 14 , CK 15 , CK 17 , CK 18 , and CK 19 in the normal submandibular gland . . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| In particular , homogeneous and constant expression of the basal differentiation markers CK 5 and CK 14 were found in both specimen , with no expression of the simple epithelium type keratin CK 8 and the suprabasal differentiation markers CK 1 and CK 10 . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| CK 1 was expressed in 10 out of 18 OF cases ; CK 5 was expressed in one OF case ; CK 14 was positively stained in 10 cases of OF ; CK 19 was positively stained in 16 OF cases ; CK 1 was expressed in three out of five AD cases ; CK 5 was expressed in one case of AD ; CK 14 was positively stained in four AD cases ; and CK 19 was positively stained in five AD cases . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| To analyze whether CK of complex or stratified epithelia are abnormally expressed in pancreas cancers , we have used polypeptide specific mouse monoclonal antibodies ( MAbs ) detecting CK 5 , CK 10 , CK 13 , CK 14 and CK 17 , and an antibody detecting CK 13 , CK 15 and CK 16 . ^^^ CK 5 , CK 14 and CK 17 were expressed by less than 5 % of cells in normal ducts , whereas CK 10 , CK 13 , CK 15 and CK 16 were not expressed at all . ^^^ In tumors , CK 14 , CK 15 / 16 and CK 17 were detected in the majority of cases studied ; CK 5 , CK 10 and CK 13 were present in a sub population of pancreas cancers . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| METHODS AND RESULTS : Antibodies to CK 4 , CK 5 and 6 , CK 7 , CK 14 , CK 8 and 18 , CK 19 , CK 20 and to vimentin were used . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| This selected population has a basal phenotype , as determined by expression of CK 5 and CK 14 and lack of expression of the differentiation specific markers prostate specific antigen ( PSA ) and prostatic acid phosphatase ( PAP ) , and has a fourfold greater ability to form colonies in vitro than the total basal population . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| The present study demonstrates that the majority of cells in embryonic urogenital sinus epithelium and developing prostatic epithelium ( rat , mouse , and human ) co expressed luminal cytokeratins 8 and 18 ( CK 8 , CK 18 ) , the basal cell cytokeratins ( CK 14 , CK 5 ) , p 63 , and the so called transitional or intermediate cell markers , cytokeratin 19 ( CK 19 ) and glutathione S transferase pi ( GSTpi ) . ^^^ In the adult prostate , the predominant basal epithelial subpopulation expressed the classical basal cell markers ( CK 5 , CK 14 , p 63 ) as well as CK 19 and GSTpi . ^^^ However , a small fraction of adult prostatic basal epithelial cells co expressed the full spectrum of basal and luminal epithelial cell markers ( CK 5 , CK 14 , CK 8 , CK 18 , CK 19 , p 63 , GSTpi ) . ^^^ Progenitor / stem cells also give rise to mature basal cells by maintaining CK 5 , CK 14 , p 63 , CK 19 , and GSTpi and losing K 8 and K 18 . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| We evaluated the expression patterns of a broad range of low to intermediate molecular weight cytokeratins ( CK 7 , CK 8 , CK 10 , CK 17 , CK 18 , CK 19 , and CK 20 ) and high molecular weight cytokeratins ( K 903 : a combination of CK 1 , CK 5 , CK 10 , and CK 14 ; and CK5 / 6 ) in these cases . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| Amyloid deposits in two cases contained a CK 5 and CK 14 pair , and in another two cases they contained both a CK 5 and CK 14 pair , and a CK 1 and CK 10 pair . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| Monoclonal antibodies directed against vimentin , CK AE1 / AE3 , CK 18 8 ( for luminal epithelial cells ) , CK 5 , CK clone 8 . 12 and CK 14 ( for basal cells ) were employed . ^^^ Basal cells were positive for CK 5 and CK clone 8 . 12 but , in contrast to findings in man , were negative for CK 14 . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| RESULTS : SMA negative ME cells in two cases also failed to display immunoreactivity for other markers , including calponin , CD 10 , smooth muscle myosin heavy chain , protease inhibitor 5 ( maspin ) , Wilms ' tumor 1 , and cytokeratins 5 , 14 , and 17 ( CK 5 , CK 14 , and CK 17 ) . ^^^ However , in one case SMA negative ME cells displayed immunoreactivities for maspin , CK 5 , CK 14 , and CK 17 . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| We reviewed histology and performed immunohistochemistry for AE1 / AE3 , 34betaE12 , CK 5 and CK 14 , Cam5 . 2 , CK 7 and CK 19 , epithelial membrane antigen ( EMA ) ( B 55 ) , smooth muscle actin ( SMA ) , S 100 , desmin , vimentin , CD 31 , CD 34 , and bcl 2 on paraffin embedded tissue from 18 MSCs , 26 PTs , and 8 fibromatoses . ^^^ Sarcomatous areas in MSCs were positive for 34betaE12 in 11 cases ; for SMA in 10 ; for CK 5 in 7 ; for CK 14 in 6 ; for Cam5 . 2 , AE1 / AE3 , and S 100 in 5 ; and for CK 7 and CK 19 in 3 . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| Paraffin embedded sections of skin tissue from 64 patients with PCA , 111 with SCA and 3 with systemic amyloidosis were analyzed immunohistochemically using 12 different polyclonal or monoclonal anti CK antibodies ( 34betaE12 , MNF 116 , LP 34 , AE1 / AE3 , anti CK 1 , CK 5 , CK 6 , CK 7 , CK 10 , CK 14 , CK 16 and CK 17 ) . ^^^ In all 12 frozen sections of PCA , the amyloid deposits were immunopositive for anti CK 5 antibody , 34betaE12 , MNF 116 and LP 34 , and seven ( 58 . 3 % ) , three ( 25 % ) and one ( 8 . 3 % ) were immunopositive for anti CK 1 , CK 14 , and CK 10 antibodies , respectively . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| CK 7 , 49 % ( 18 / 37 ) ; CK 20 , 35 % ( 13 / 37 ) ; CK 1 , CK 5 , CK 10 , CK 11 , CK 13 , CK 14 , CK 15 , CK 16 , and CK 17 were all negative in MBs . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| Differentiation of culture expanded hREC was further characterized by gene expression analysis of cytokeratins CK 5 , CK 13 , CK 14 and CK 18 using semi quantitative real time RT PCR technique . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| However , in the second tumour the expression of CK 14 in most of vacuolated cells and alpha smooth muscle actin ( alpha SMA ) in non vacuolated cells , alone or in combination with CK 5 suggested a myoepithelial immunophenotype for both cell types . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| Thirty nine cases of AOT from three Oral Diagnosis services ( Brazil , Mexico and Guatemala ) were studied , considering their clinical , radiographic , and histological features and immunohistochemical expression of cytokeratins ( AE1 / AE3 , 34betaE12 , CK 1 , CK 5 , CK 6 , CK 7 , CK 8 , CK 10 , CK 13 , CK 14 , CK 16 , CK 18 , and CK 19 ) , vimentin and Ki 67 . ^^^ All cases were positive for AE1 / AE3 , 34betaE12 , CK 5 , CK 14 and CK 19 . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Immunohistochemically , all the tumours showed a constant expression of high molecular weight cytokeratins ( Ck ) Ck 5 and Ck 14 , p 63 , SMA and vimentin . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| Ck 5 , 13 , and 14 were positive for both groups , nevertheless suprabasal staining for Ck 14 was more evident in mucositis than in the control group . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| In addition to CK 34ssE12 , these basal cells also completely lacked the expression of other phenotypic markers , including CK 5 , CK 14 , p 63 , and maspin , in contrast to adjacent basal cells , which were strongly positive for these markers . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| The overall positive rates were 84 . 7 % for CK 4 , 3 . 2 % for CK 5 , 28 . 7 % for CK 6 , 71 . 1 % for CK 7 , 96 . 6 % for CK 8 , 0 % for CK 10 , 81 . 6 % for CK 13 , 0 . 3 % for CK 14 , 4 . 1 % for CK 16 , 0 % for CK 17 , 99 . 4 % for CK 18 , 89 . 7 % for CK 19 , and 30 . 0 % for CK 20 . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| In this study , we used tissue microarray to examine the expression of basal cytokeratins ( CK ) ( CK 5 and CK 14 ) and luminal CK ( CK8 / 18 ) , epidermal growth factor receptor , c kit , hormone receptors ( HRs ) , p 53 , and Her2 / neu in 776 consecutive patients diagnosed with invasive breast carcinoma from January 1993 to December 1998 and categorized these cases into 5 subgroups ( basal like , HR expressing , Her2 / neu overexpressing , HR and Her2 / neu expressing , and null subtypes negative for all markers ) , based on the immunohistochemical data . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| KA 1 antibody , which by one and two dimensional gel electrophoresis and immunoblotting was shown to bind preferentially to cytokeratin 14 in a complex with cytokeratin 5 , reacted with the nonproliferating myoepithelium of normal gland , cystic disease , adenosis , papilloma , fibroadenoma , and in situ carcinoma ; it also reacted with a subpopulation of proliferating cells in sclerosing adenosis and epitheliosis ( papillomatosis ) but was negative with the tumor cells of all preinvasive and most invasive carcinomas . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| The cytokeratins 5 , 7 , 8 , 14 , 18 , and 19 are expressed in the bladder epithelium as a function of developmental age with cytokeratin 7 , 8 , 18 , and 19 being coexpressed at 15 days ' gestation followed by cytokeratin 5 at 17 days ' gestation and cytokeratin 14 in the newborn bladder . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| In the first family , linkage was found to chromosome 17 markers flanking the keratin 14 gene ( D17S74 : Zmax = +2 . 45 , theta = 0 . 10 ; COL1A1 : Zmax = +0 . 97 , theta = 0 . 00 ) and markers near the keratin 5 gene on chromosome 12 were excluded ( D12S17 : Z less than 2 . 0 , theta = 0 . 08 ; COL2A1 : Z less than 2 . 0 , theta = 0 . 13 ) . ^^^ In contrast , the second family showed linkage to the region containing the keratin 5 gene ( D12S17 : Zmax = +1 . 37 , theta = 0 . 08 ; COL2A1 : Zmax = +0 . 33 , theta = 0 . 15 ) and was not linked to the keratin 14 gene ( D17S74 : Z less than 2 . 0 , theta = 0 . 14 ) . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| Keratin 14 ( K 14 ) expression , determined by protein synthesis and mRNA levels , was dramatically down regulated in the cervical SCC lines while keratin 5 ( K 5 ) expression was not . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| In this report , we describe in vitro filament assembly studies on headless , tailless , headless / tailless , and R / K L L E G E truncated mutants of keratin 5 and its partner keratin 14 . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| A comparison of the keratin 5 sequence with coexpressed keratin 14 reveals an absence of sequence conservation in regulatory regions and suggests that common sequence elements may not be necessary for coordinate expression of type 1 and type 2 keratin partners . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| The patterns of binding of antibodies by these epithelia indicated that ( a ) keratin 19 was expressed by all epithelia , and ( b ) rests of Malassez also expressed keratin 5 but not large amounts of other keratins and ( c ) epithelial proliferation in periapical lesions was associated with increased expression of keratin 14 , a marker of stratifying epithelia , new expression of keratins 4 and 13 , differentiation markers for non cornifying epithelia and variable , low levels of keratins 8 and 18 , markers of simple epithelia . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| The disorder results from a mutation in either the keratin 5 or keratin 14 gene , which encode the peptide components of the obligate heterodimeric keratin intermediate filaments of the basal cell . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| Recent advances in molecular biology have enabled the association of epidermolysis bullosa simplex ( EBS ) with point mutations of keratin 14 and / or keratin 5 genes to be established . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| Keratin 5 and keratin 14 have been touted as the hallmarks of the basal keratin networks of all stratified squamous epithelia . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| Recently , it was discovered that the more severe types , Dowling Meara and Koebner , are genetic disorders of the basal epidermal keratins , keratin 5 ( K 5 ) and keratin 14 ( K 14 ) . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| Point mutations in the keratin intermediate filament genes for keratin 5 or keratin 14 are known to cause hereditary skin blistering disorders such as epidermolysis bullosa simplex , in which epidermal keratinocytes are extremely fragile and the skin blisters on mild trauma . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| It is now well established that the three major subtypes of EBS are genetic disorders of the basal epidermal keratins , keratin 5 ( K 5 ) and keratin 14 ( K 14 ) . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| In the AP keratin 14 was basally localized by 1 day postnatal but keratin 5 and 7 did not colocalize to the basal cells until days 9 and 12 , respectively . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| The expression of keratin 5 , the obligate copolymer of keratin 14 , was slightly reduced . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| Despite a lack of normal keratin 5 molecules , and an effective doubling of abnormal molecules , available for heterodimerization with keratin 14 during IF formation , there were no significant differences in the clinical severity or the ultrastructural organization of the keratin IF cytoskeleton of the homozygous individual . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| By contrast , only slight alterations in mRNA levels were observed for one differentiation marker ( keratinocyte transglutaminase ) and for keratin 5 , keratin 14 , AP 2 or glyceraldehyde phosphate dehydrogenase . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| The keratin 5 and keratin 14 genes encode proteins that form the primary structural components of the basal epidermal keratinocytes , mutations in either of these genes can cause epidermolysis bullosa simplex . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| These cells were transduced with retroviral vectors containing the factor 9 cDNA under the control of a cytomegaloviral ( CMV ) promoter / enhancer alone or as hybrids with either the human papilloma virus 16 ( HPV 16 ) , keratin 14 ( hK 14 ) or keratin 5 ( hK 5 ) regulatory elements . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| The mutations are located in the keratin 14 L 12 linker region ( D273G ) , the keratin 5 L 12 linker ( M327K and D328H ) , and the H 1 domain of keratin 5 ( P156L ) . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| Epidermolysis bullosa simplex ( EBS ) is a group of autosomal dominant inherited skin diseases caused by mutations in either the keratin 5 ( K 5 ) or the keratin 14 ( K 14 ) genes and characterized by development of intraepidermal skin blisters . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| Keratin 5 and keratin 14 are known to be essential for the basal keratinocyte cytoskeleton and are defective in several forms of epidermolysis bullosa simplex . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| One patient carries a point mutation in keratin 14 ( converting arginine at position 125 to histidine ) and the other has a novel point mutation in keratin 5 ( converting serine at position 181 to proline ) . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| Skin fragility is caused by expression of either an abnormal keratin 5 or an abnormal keratin 14 protein , which compromises the structure and function of the keratin cytoskeleton of basal cells . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| The POU homeodomain factors Skn 1a and Tst 1 , which are expressed in epidermis , may play a role in the suprabasal repression of the keratin 5 and 14 genes because keratin 14 mRNA expression persists in suprabasal cells in Skn 1 / Tst 1 double knockout mice . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| The major subtypes of EBS , including EBS Dowling Meara ( EBS DM ) , are caused by mutations of the basal keratin genes , keratin 5 ( KRT 5 ) or keratin 14 ( KRT 14 ) . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| Most cases are caused by mutations in the genes encoding keratin 5 ( K 5 ) and keratin 14 ( K 14 ) and are characterized by cytolysis within the basal layer of the epidermis . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| Ultraviolet B irradiation increases keratin 5 and keratin 14 expression through epidermal growth factor receptor of SV 40 transformed human keratinocytes . ^^^ UVB irradiation ( 10 mJ / cm ( 2 ) ) increased keratin 5 and keratin 14 mRNAs and proteins without affecting cell viability . ^^^ Upregulation of keratin 5 and keratin 14 was dependent on the dose of radiation : the effect was observed at 5 mJ / cm ( 2 ) and the maximal effect was observed at 10 mJ / cm ( 2 ) . ^^^ The stimulatory effects on keratin 5 and keratin 14 expression were also observed in cultured normal human keratinocytes ( NHK ) and HaCaT keratinocytes . ^^^ The tyrosine kinase inhibitor , genistein , and the epidermal growth factor ( EGF ) receptor inhibitor , AG 1429 , significantly suppressed the increase in expression of keratin 5 and keratin 14 by SVHK . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| By this approach , we demonstrated that ultraviolet B upregulated the transcription of keratin 19 gene and to a lesser extent the keratin 6 , keratin 5 , and keratin 14 genes . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| Mutations in the epidermal keratin genes , keratin 5 or keratin 14 lead to the disruption of keratin filament assembly , resulting in an autosomal dominant inherited blistering skin disease , epidermolysis bullosa simplex ( EBS ) . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| All three EBS subtypes are caused by mutations in either keratin 5 or keratin 14 , the major keratins expressed in the basal layer of the epidermis . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| Blot overlays pointed to molecular interactions of synemin with desmin , vimentin , GFAP and keratin 5 and 6 , but not with keratin 14 . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| The three major clinical subtypes ( Weber Cockayne , Koebner and Dowling Meara ) are all caused by mutations in either the keratin 5 ( KRT 5 ) or keratin 14 ( KRT 14 ) gene . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| In this study , we generated transgenic mice that overexpressed either a constitutively active human c src mutant ( src ( 530 ) ) or a wild type human c src ( src ( wt ) ) in epidermal basal cells driven by human keratin 14 ( HK 14 ) or bovine keratin 5 ( BK 5 ) promoters , respectively . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| Keratins are particularly important for the structural integrity of skin , and several genetically inherited blistering diseases have been linked to mutations in keratin 5 and keratin 14 . ^^^ Western blotting with specific monoclonal antibodies revealed the formation of stable high molecular weight `` aggregates ' ' containing keratin 14 and / or keratin 5 . ^^^ Purification and analysis of these aggregates by one and two dimensional gel electrophoresis and mass spectrometry confirmed the presence of keratin 14 and keratin 5 and indicate that at least some of the aggregates are composed of keratin 14 keratin 14 , keratin 14 keratin 5 , or keratin 5 keratin 5 dimers . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| EBS is caused by mutations in either keratin 5 or keratin 14 , the major keratins expressed in the basal layer of the epidermis . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| Inherited mutations in the intermediate filament ( IF ) proteins keratin 5 ( K 5 ) or keratin 14 ( K 14 ) cause epidermolysis bullosa simplex ( EBS ) , in which basal layer keratinocytes rupture upon trauma to the epidermis . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| Among these patients , 18 were found to harbor heterozygous mutations in the keratin 5 or keratin 14 genes , KRT 5 and KRT 14 , respectively , whereas in 14 cases , the disease was associated with mutations in both alleles of the plectin gene . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| Immunohistochemical analysis showed that the suprabasal keratinocytes of the TAK 1 deficient epidermis expressed keratin 5 and keratin 14 , which are normally confined to the basal layer . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| The KRT 5 and KRT 14 genes encode the proteins keratin 5 and 14 , respectively , which are the primary structural components of the 10 nm intermediate filaments of the mitotic epidermal basal cells . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| Epidermolysis bullosa simplex ( EBS ) is caused by defective assembly of keratin intermediate filaments in basal keratinocytes and recent studies indicated causal mutations in the keratin KRT 5 and KRT 14 genes . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| In patients with the major forms of epidermolysis bullosa simplex , either of the keratin genes KRT 5 or KRT 14 is mutated . ^^^ Consequently , mutation detection in epidermolysis bullosa simplex has mostly been carried out on cDNA synthesized from KRT 5 and KRT 14 transcripts in mRNA isolated from skin biopsies . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| Epidermolysis bullosa simplex is a hereditary skin blistering disorder caused by mutations in the KRT 5 or KRT 14 genes . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| Epidermolysis bullosa simplex is a group of blistering skin disorders caused by defects in one of the keratin genes , KRT 5 and KRT 14 . ^^^ Previously reported KRT 5 and KRT 14 mutations are clustered in several hotspots , namely the rod ends of the 1A and 2B domains and in the non helical linker region L 12 . ^^^ Therefore , genomic KRT 5 and KRT 14 mutation analysis was initially limited to these hotspots . ^^^ In two patients , with the Koebner and the Weber Cockayne subtypes of epidermolysis bullosa simplex respectively , we could , however , not identify any mutation in one of the hotspot domains of KRT 5 and KRT 14 . ^^^ Using the complete genomic mutation detection system for both KRT 5 and KRT 14 , we identified four novel KRT 5 mutations ( IVS 1 1G > C , K404E , A438D , E475K ) , two of which are outside the KRT 5 hotspot domains , and three novel KRT 14 mutations ( IVS4+1G > A , L408M , L130P ) . . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| Mutations in genes keratin 5 ( KRT 5 ) and 14 ( KRT 14 ) encoding the basal type keratin intermediate filaments have been identified in epidermolysis bullosa simplex ( EBS ) families and are likely to cause skin fragility . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| The mutation was identified by direct sequencing of polymerase chain reaction ( PCR ) amplified genomic DNA encoding the exons of the KRT 5 and KRT 14 genes , and confirmed by mismatch allele specific PCR , followed by restriction enzyme digestion with Tsp 509 1 . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| Novel and recurrent mutations in keratin KRT 5 and KRT 14 genes in epidermolysis bullosa simplex : implications for disease phenotype and keratin filament assembly . ^^^ Epidermolysis bullosa simplex ( EBS ) is a group of autosomal dominant genetic skin disorders caused by mutations of the keratin genes KRT 5 and KRT 14 . ^^^ We investigated 27 EBS patients and families of mainly German origin by sequence analysis of the entire coding sequences of KRT 5 and KRT 14 and identified 12 novel and seven previously reported mutations within the KRT 5 and KRT 14 genes . ^^^ |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02533 and P13647 |
Pubmed |
SVM Score :0.0 |
| NA |
|