Pubmed abstracts for Protein-Protein Interaction search result :


Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
We have located a cis acting element ( alpha 1 TAE ) within the promoter sequences of the rat collagen alpha 1 ( 1 ) gene ( COL1A1 ) 1600 bases upstream of the transcription start site which mediates transcriptional activation by transforming growth factor beta ( TGF beta ) . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
Neointima formation and TGF beta 1 immunoreactivity were correlated with increases in fibronectin , collagen alpha 2 ( 1 ) , and collagen alpha 1 ( 3 ) gene expression . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
The effect of TGF beta 1 on the expression of the basement membrane genes , fibronectin , laminin B 1 , and collagen alpha 1 ( 4 ) , was examined . ^^^ Treatment of these differentiated cells with TGF beta 1 induced the expression of fibronectin mRNA to levels seen in TGF beta treated , undifferentiated keratinocytes but only marginally increased the expression of collagen alpha 1 ( 4 ) and laminin B 1 mRNA . ^^^ The inclusion of cycloheximide in the protocol indicated that TGF beta induction of collagen alpha 1 ( 4 ) mRNA was signaled by proteins already present in the cells but that TGF beta required the synthesis of a protein ( s ) to fully induce expression of fibronectin and laminin B 1 mRNA . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
TGF beta and collagen alpha 1 ( 1 ) gene expression are increased in hepatic acinar zone 1 of rats with iron overload . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
RNA was isolated after the addition of varying amounts of TGF beta 1 to the cells in culture for varying periods of time , and collagen alpha 1 ( 4 ) RNA was quantitated by filter hybridization . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
This region harbored a 14 bp element similar to a TGF beta responsive element present in the promoters of collagen alpha 1 ( 1 ) , alpha 2 ( 1 ) , elastin , and growth hormone . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
The results demonstrate that transforming growth factor beta mRNA is expressed by periosteum derived cells and that exogenous transforming growth factor beta 1 acts to upregulate expression of the gene encoding collagen alpha 1 ( 2 ) . ^^^ No synergy was demonstrated between transforming growth factor beta 1 and bone morphogenetic protein 3 in the ability to upregulate expression of the collagen alpha 1 ( 2 ) gene . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
Dose dependent induction by TGF beta 1 was observed for both HSP 47 mRNA and collagen alpha 1 ( 1 ) mRNA , and actinomycin D inhibited this increase of HSP 47 mRNA . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
Exposure of VSMCs from WKY to the TGF beta isoforms beta 1 , beta 2 , or beta 3 induced rapid , transient elevations in mRNAs encoding collagens alpha 1 ( 1 ) , alpha 2 ( 1 ) , and alpha 1 ( 3 ) ; maximum increases were apparent by 2 hours and ranged from twofold [ collagen alpha 1 ( 3 ) ] to ninefold [ collagen alpha 1 ( 1 ) ] . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
Elevated expression of collagen alpha 1 ( 3 ) chain and TGF beta 1 mRNAs , and its reversal by saralasin were also demonstrated in stretched SMCs . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
In a similar manner to PGE 2 , 11 deoxy PGE 1 decreases basal and TGF beta induced type 1 collagen alpha 1 ( COL ) mRNA , basal and IL 1beta induced LO mRNA while it increases COX 1 mRNA . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
KRH 594 ( 10 mg / kg / day ) also suppressed the injury induced gene expressions for transforming growth factor beta 1 and fibronectin and reduced collagen alpha 1 ( 1 ) and alpha 1 ( 3 ) mRNA levels for the first 7 days after injury . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
Although TGFbeta 1 is a potent inducer of collagen alpha 1 ( 1 ) expression in vitro and elevated levels of TGFbeta 1 are found in patients and experimental animals with hepatic fibrosis and cirrhosis , the role of increased TGFbeta 1 in the initiation of hepatic stellate cell activation in vivo is unknown . ^^^ The TGFbeta 1 mRNA levels in the wild type mice were proportional to the collagen alpha 1 ( 1 ) mRNA levels . a sma , a marker of hepatic stellate cell activation , was expressed earlier and at a higher level in wild type mice than TGFbeta ko mice after CCl 4 treatment . ^^^ The Ad TGFbeta 1 infected mice had 14 fold higher hepatic TGFbeta protein levels and 15 fold higher collagen alpha 1 ( 1 ) mRNA levels than the Ad LacZ infected control mice . ^^^ Collagen alpha 1 ( 1 ) mRNA levels were proportional to the transgenic TGFbeta 1 mRNA levels , while the endogenous TGFbeta 1 was only slightly higher than in the controls . ^^^ CONCLUSIONS : Absence of TGFbeta 1 inhibits hepatic collagen alpha 1 ( 1 ) mRNA and alpha sma protein expression by the toxic stimulus CCl 4 , and targeted TGFbeta 1 overexpression increases collagen alpha 1 ( 1 ) mRNA and alpha sma protein levels in the liver in vivo . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
PTX also suppressed both basal and TGF beta 1 augmented collagen alpha 1 ( 1 ) and collagen alpha 1 ( 3 ) mRNA levels beginning at 24 h , and attenuated both basal and TGF beta 1 ( 5 ng / ml ) stimulated total collagen synthesis at 48 h . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
Bacterial cell wall polymers directly stimulated collagen alpha 1 ( 1 ) , TGF beta 1 , IL 1beta , and IL 6 mRNA expression in the intestinal myofibroblasts derived from both normal and inflamed cecum . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
Significant increases in mRNA for fibronectin ( 7 . 5 fold , P < 0 . 01 ) , heparan sulfate proteoglycan core protein ( 53 fold , P < 0 . 001 ) , laminin beta 1 ( 12 fold , P < 0 . 001 ) , collagen alpha 1 ( 4 ) ( 17 fold , P < 0 . 001 ) , collagen alpha 1 ( 3 ) ( fourfold , P < 0 . 001 ) , and MMP 9 ( twofold , P < 0 . 05 ) were observed after perfusion with TGF beta 1 . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
RESULTS : The mRNAs encoding TGF beta 1 ( in both mouse and human ) , entactin , fibronectin , and the collagen alpha 1 ( 4 ) and alpha 2 ( 4 ) chains were significantly induced in total kidney as a function of Alport renal disease progression . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
The candidate genes studied were the androgen receptor , type 1 collagen A 1 ( COLIA 1 ) , COLIA 2 , COLIIA 1 , vitamin D receptor ( VDR ) , colony stimulating factor 1 , calcium sensing receptor , epidermal growth factor ( EGF ) , estrogen receptor 1 ( ESR 1 ) , fibrillin type 1 , insulin like growth factor 1 , interleukin 1 alpha ( IL 1alpha ) , interleukin 4 ( IL 4 ) , interleukin 6 ( IL 6 ) , interleukin 11 ( IL 11 ) , osteopontin , parathyroid hormone ( PTH ) , PTH related peptide , PTH receptor type 1 ( PTHR 1 ) , transforming growth factor beta 1 , and tumor necrosis factors alpha and beta . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
We have previously identified a potential TGF beta activation element ( TAE ) in the rat collagen alpha 1 ( 1 ) promoter at 1624 upstream of the transcriptional start site [ Ritzenthaler et al . , 1991 , 1993 ] . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
At 2 mo , inulin clearance , urinary albumin excretion , fractional albumin clearance , glomerular volume , and glomerular content of immunoreactive transforming growth factor beta ( TGF beta ) and collagen alpha 1 ( 4 ) all were significantly increased in unsupplemented D compared with age matched nondiabetic controls . ^^^ VC suppressed urinary albumin excretion , fractional albumin clearance , and glomerular volume but not glomerular or tubular TGF beta or glomerular collagen alpha 1 ( 4 ) content . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
Urinary albumin excretion , fractional albumin clearance , urinary transforming growth factor beta ( TGF beta ) excretion , glomerular volume , glomerular content of immunoreactive TGF beta , and collagen alpha 1 ( 4 ) and renal cortical malondialdehyde ( MDA ) levels were significantly higher in D WT mice compared with corresponding values in D SOD mice . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
RESULTS : TGF beta stimulated collagen alpha 1 ( 1 ) mRNA expression of HPMC was inhibited by dipyridamole in a dose dependent manner . ^^^ The addition of PD 98059 , which blocked activation of ERK1 / 2 , suppressed TGF beta induced collagen alpha 1 ( 1 ) mRNA expression in a dose dependent manner . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
Transforming growth factor beta ( TGF beta ) stimulates protein complex formation on a TGF beta response element ( TAE ) found in the distal portion ( 1624 ) of the collagen alpha 1 ( 1 ) promoter . ^^^ In conclusion , we have identified YB 1 as a protein that interacts with a TGF beta response element in the distal region of the collagen alpha 1 ( 1 ) gene . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
Cellular ANG , TGF beta 1 , and collagen alpha 1 ( type 4 ) mRNA levels were determined by Northern blot analysis or by reverse transcriptase PCR assay . ^^^ The results showed that stable transfer of antisense ANG cDNA into IRPTC suppressed the basal TGF beta 1 and collagen alpha 1 ( type 4 ) mRNA expression and blocked the stimulatory effect of high glucose ( i . e . , 25 mM ) on TGF beta 1 and collagen alpha 1 ( type 4 ) mRNA expression and induction of IRPTC hypertrophy . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
Renal cortical mRNA levels of TGF beta 1 , collagen alpha 1 ( 4 ) , collagen alpha 1 ( 1 ) , decorin , and the household gene glyceraldehyde 3 phosphate dehydrogenase in biopsy specimens taken during acute rejection episodes were quantified by real time polymerase chain reaction . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
In cells lacking Sp 1 and KLF 6 , transfected KLF 6 and Sp 1 cooperatively transactivated the endoglin promoter and those of collagen alpha 1 ( 1 ) , urokinase type plasminogen activator , TGF beta 1 , and TGF beta receptor type 1 . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
RESULTS : TGF beta stimulated collagen alpha 1 ( 1 ) mRNA expression of HPMC was inhibited by PTX . ^^^ The addition of PD 98059 and SB 203580 , which blocked the activation of ERK1 / 2 and p 38 ( HOG ) , respectively , suppressed the TGF beta induced collagen alpha 1 ( 1 ) mRNA expression . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
The activation of primary and immortalized human stellate cells after incubation with apoptotic bodies , as well as their fibrogenic activity , was indicated by an increase in alpha smooth muscle actin ( primary cells ) , TGF beta 1 , and collagen alpha 1 ( 1 ) mRNA ( primary and immortalized cells ) . ^^^ The profibrogenic response was dependent upon apoptotic body engulfment , because nocodazole , a microtubule inhibiting agent , blocked both the engulfment and the increase of TGF beta 1 and collagen alpha 1 ( 1 ) mRNA . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
Liver hydroxyproline content , the mRNA expression of collagen alpha 1 ( 1 ) and alpha smooth muscle actin ( alpha SMA ) , and plasma levels of transforming growth factor beta 1 ( TGF beta 1 ) were significantly reduced by olmesartan treatment , suggesting that olmesartan improved liver fibrosis . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
The effect of NS 398 , a selective COX 2 inhibitor , and PGE ( 2 ) on TGF beta 1 mediated fibrogenesis was examined by measuring mRNA levels of collagen alpha 1 ( 1 ) . ^^^ Importantly , TGF beta 1 mediated induction of collagen alpha 1 ( 1 ) was markedly suppressed by the addition of exogenous PGE ( 2 ) . ^^^ Prostaglandin E 2 inhibits transforming growth factor beta 1 mediated induction of collagen alpha 1 ( 1 ) in hepatic stellate cells . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
Levels of collagen alpha 1 gene expression , hydroxyproline , angiotensin 2 and TGF beta 1 proteins , and TGF beta 1 mRNA were measured on the colonic tissue . ^^^ RESULTS : In chronic colitis , captopril significantly reduced the score of macroscopic and histologic lesions , as well as the colonic tissue levels of collagen alpha 1 , hydroxyproline , ANG 2 and TGF beta 1 proteins , and TGF beta 1 mRNA . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
RT PCR revealed IL 10 gene therapy to reduce liver transforming growth factor beta 1 , tumor necrosis factor alpha , collagen alpha 1 , cell adhesion molecule , and tissue inhibitors of metalloproteinase mRNA upregulation . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
RESULTS : The mRNA expressions of collagen alpha 1 ( 1 ) , MMP 1 , TIMP 1 , TGF beta 1 were not changed by 17beta estradiol stimulations at a concentration of 10 ( 12 ) to 10 ( 8 ) M . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
Seventeen days after UUO , significant increases in collagen alpha 2 ( 1 ) , collagen alpha 1 ( 4 ) , TGF beta 1 and endoglin mRNA levels were detected in L kidneys vs NL kidneys . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
Finally , RT PCR measurements showed a significant upregulation of genes involved in tissue remodeling and fibrosis ( matrix metalloproteinases , collagen alpha 1 ) , suppressor of cytokines signaling 1 , and the inflammatory cytokines ( IL 1beta , IL 6 , TNF alpha , and TGF beta ) in the livers of rats fed MCD . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
RT PCR revealed that alpha MSH gene therapy attenuated the liver TGF beta 1 , collagen alpha 1 , and cell adhesion molecule mRNA upregulation . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
Reverse transcription polymerase chain reaction revealed that alpha MSH gene therapy attenuated the liver transforming growth factor beta 1 , collagen alpha 1 and cell adhesion molecule mRNA upregulation . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
RT PCR revealed that IL 10 gene therapy attenuated liver TGF beta 1 , collagen alpha 1 , fibronectin , and cell adhesion molecule mRNA upregulation . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
However , there was a marked decrease in hepatic factors involved in regulating fibrogenesis ( collagen alpha 1 ( 1 ) , transforming growth factor beta 1 , alpha smooth muscle actin , and tissue inhibitor of metalloproteinase 1 expression and alpha smooth muscle actin content ) and inflammation ( macrophage chemoattractant protein 1 and interleukin 8 expression ) ( P < . 05 , compared with values before weight loss ) . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
We also examined the effects of IL 7 , PKC inhibitor , and STAT inhibitor on the expressions of TGF beta 1 and type 1 collagen alpha 1 chain ( col1A1 ) m RNA by using real time RT PCR . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
Northern analysis showed abundant TGF beta 1 mRNA in AOM induced tumors , but not in paired mucosa . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
We postulated that TGF beta 1 and TbetaR 2 alterations may also be involved in mouse colon tumorigenesis induced by the chemical carcinogen , azoxymethane ( AOM ) . ^^^ In the present study , normal colon tissues and AOM induced colon tumors from SWR / J mice were analyzed for mutational changes in the TbetaR 2 gene , and the expression and localization of TGF beta 1 and TbetaR 2 were examined by reverse transcription polymerase chain reaction ( RT PCR ) and immunohistochemisty . ^^^ These findings suggest that alterations in the expression of TGF beta 1 and TbetaR 2 may be involved in the pathogenesis of colon tumors induced by AOM in mice . . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
We hypothesize that alterations in the TGF beta pathway contribute to differential sensitivity of mice to the colon carcinogen azoxymethane ( AOM ) . ^^^ Twenty four weeks after AOM exposure , mutational analysis of TGF beta type 2 receptor ( TbetaR 2 ) from normal colons and from tumors showed no AOM induced alterations . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
To determine the expression and activation status of TGF beta 1 in chemically induced tumors , 6 wk old A / J mice were injected intraperitoneally with either azoxymethane ( AOM ) ( 10 mg / kg body weight , once a week for 6 wk ) or normal saline solution , and colon tumors were isolated 24 wk following the last injection . ^^^ To gain further insight into the functionality of the TGF beta 1 pathway , cDNA microarrays were performed and the expression levels of a panel of 21 TGF beta 1 specific target genes were determined in AOM induced tumors that overexpress the ligand . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
Constructs of promoter and first intron sequences associated with the luciferase reporter gene were used to delineate the gene sequences involved in TGF beta 1 control of human COL2A1 gene transcription . ^^^ Cotransfection of these DNA fragments with a TbetaRII / I cDNA hybrid receptor , capable of inducing a TGF beta 1 dominant negative effect , showed that TGF beta 1 inhibits specifically COL2A1 gene transcription in RAC by a 63 bp proximal promoter . ^^^ Transfection of decoy Sp binding oligonucleotides corroborated the implication of the proximal promoter in the TGF beta 1 induced inhibition of COL2A1 gene transcription . ^^^ These findings suggest that TGF beta 1 inhibition of COL2A1 gene transcription in RAC is mediated by an increase of the Sp3 / Sp1 ratio and by the repression of Sp 1 transactivating effects on that gene . . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
Because we demonstrated previously that Sp 1 was a strong activator of COL2A1 gene expression via the 63 / +1 promoter region , whereas Sp 3 overexpression blocked Sp 1 induced promoter activity and inhibited COL2A1 gene transcription , we conclude that IL 1 beta down regulation of that gene , as we found previously for transforming growth factor beta 1 , is mediated by an increase in the Sp3 / Sp1 ratio . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
We observed that Smad2 / 3 associated with Sox 9 in a TGF beta dependent manner and formed the transcriptional complexes with SOX 9 on the enhancer region of COL2A1 . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
Asporin suppresses TGF beta mediated expression of the genes aggrecan ( AGC 1 ) and type 2 collagen ( COL2A1 ) and reduced proteoglycan accumulation in an in vitro model of chondrogenesis . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
Transforming growth factor ( TGF ) beta activated kinase 1 mimics and mediates TGF beta induced stimulation of type 2 collagen synthesis in chondrocytes independent of Col2a1 transcription and Smad 3 signaling . ^^^ Stimulation by TAK1a or TGF beta did not require increased Col2a1 mRNA , and TAK 1 actually reduced Col2a1 mRNA in parallel with the cartilage markers , SRY type HMG box 9 ( Sox 9 ) and aggrecan . ^^^ Thus , TAK 1 increased target gene expression ( Col2a1 ) by translational or posttranslational mechanisms as a Smad 3 independent response shared by TGF beta 1 and BMP2 . . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
Wnt8c further enhanced canonical bone morphogenetic proteins ( BMP 2 ) induced expression of Runx 2 and col10a1 while Wnt8c and Wnt9a inhibited TGF beta induced expression of Sox 9 and col2a1 . ^^^
Interacting proteins: P02458 and P01137 Pubmed SVM Score :0.0
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