Pubmed abstracts for Protein-Protein Interaction search result :


Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.99218658
As a follow up to our previous findings that gp 17 binds to CD 4 with high affinity and interferes with both HIV 1 gp 120 binding to CD 4 and syncytium formation , we investigated whether gp 17 could affect the T lymphocyte apoptosis induced by a separate ligation of CD 4 and TCR . 0.99218658^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :1.013776
We previously demonstrated that gp 17 binds to CD 4 with high affinity and strongly inhibits T lymphocyte apoptosis induced by sequential cross linking of CD 4 and T cell receptor ( TCR ) . 1.013776^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
To establish further the correspondence of gp 17 and GCDFP 15 / PIP / SABP , the latter was expressed in bacteria from a cloned cDNA and purified by affinity chromatography to either anti gp 17 mAb Sepharose or CD 4 Sepharose . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
We have previously isolated from human seminal plasma a CD 4 ligand , the gp 17 glycoprotein , which shares sequence identity with three previously identified proteins : secretory actin binding protein ( SABP ) from seminal plasma , gross cystic disease fluid protein 15 ( GCDFP 15 ) and prolactin inducible protein ( PIP ) from breast tumor cells . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
Differential antibody reactivity and CD 4 binding of the mammary tumor marker protein GCDFP 15 from breast cyst and its counterparts from exocrine epithelia . ^^^ We compare here the ability of independently purified GCDFP 15 , SABP and gp 17 and of recombinant PIP both to cross react with a panel of monoclonal antibodies ( MAbs ) raised against GCDFP 15 or gp 17 , respectively , and to bind to CD 4 . ^^^ Surface plasmon resonance analysis also showed that anti GCDFP 15 and anti gp 17 antibodies inhibit the binding of CD 4 to GCDFP 15 and gp 17 , respectively , to different extents . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
GCDFP 15 / gp17 is a ligand of CD 4 and is a potent inhibitor of T cell apoptosis induced by sequential CD4 / T cell receptor triggering . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
Isolation from a human seminal vesicle library of the cDNA for gp 17 , a CD 4 binding factor . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
CD 4 mediated anchoring of the seminal antigen gp 17 onto the spermatozoon surface . ^^^ Immunoblotting experiments with monoclonal antibodies indicated that SDS lysates from spermatozoa contain proteins with the same electrophoretic and antigenic properties of CD 4 and gp 17 . ^^^ Anti CD 4 mouse monoclonal antibodies were used to coprecipitate from NP 40 lysate proteins reacting with chicken anti gp 17 antibodies . ^^^ The possible function of CD 4 and gp 17 on male germ cells is discussed . . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
Specifically , we examined T cell subsets separated according to expression of CD 4 , CD 8 , Thy 1 , B 220 , alpha / beta T cell receptor ( TcR ) and gamma / delta TcR in PP and IEL of MRL / lpr mice at 6 , 12 and 21 weeks of age . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
We examined the TcR gamma delta usage and co expression of CD 5 , CD 4 , CD 8 and major histocompatibility complex ( MHC ) class 2 on isolated sheep IEL and lamina propria lymphocytes ( LPL ) , and compared them with the TcR gamma delta + cells in peripheral blood , intestinal lymph and jejunal Peyer ' s patches ( PP ) . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
As in responses to CD 2 mAbs , cross linking of CD 4 and / or CD 8 to the TCR CD 3 complex but not CD 3 cross linking alone promoted pp 19 dephosphorylation . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
These cells are particularly prevalent in Peyer ' s patches ( PP ) , representing 17 + / 3 % of PP CD 4 T cells , whereas they are much less prevalent in spleen , lymph nodes , lamina propria , or peritoneum . ^^^ Phenotypic studies of fresh isolated PP T cells demonstrate that all PP CD 4 T cells ( both Thy 1 and Thy 1+ ) express CD 3 , alpha beta TCR , and CD 5 ( Lyt 1 ) , whereas none coexpress CD 8 ( Lyt 2 ) . ^^^ Thy 1 and Thy 1+ CD 4 T cell lines generated from PP also coexpress CD 3 and alpha beta TCR , but are heterogeneous in expression of CD 5 and again do not coexpress CD 8 . ^^^ Finally , immunohistochemical studies suggested that many of the CD4+ T cells in PP germinal centers were Thy 1 , indicating that Thy 1 and Thy 1+ CD 4 T cells differ in their distribution within the PP . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
The use of the P . pastoris secretory pathway allowed instead the accumulation in the culture medium of a GCDFP 15 / gp17 species which retained the ability to bind to CD 4 and also most of the biochemical and immunological properties of the native protein . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
A study of the lymphocyte populations using Coulter flux cytometry in 35 PP patients ( 32 women , 3 men , average age 46 years ) did not show any modification in the number of CD 4 and CD 8 lymphocytes by comparison with the controls . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
On the other hand , CD 4 cells localized consistently more efficiently than CD 8 cells in both PP and LN of adult NOD and BALB / c mice , whereas both populations migrated roughly equivalently in LN of adult DBA / 2 , CBA , and C57BL / 6 mice . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
In vivo treatment with mAb HP2 / 1 against the integrin alpha 4 subunit inhibited almost entirely CD45RC CD 4 T cell migration into the PP ( 98 . 1 % ) , intestine ( 87 . 1 % ) , MLN ( 89 . 1 % ) and thymus ( 93 . 5 % ) ; migration into the CLN was only reduced by half . ^^^ The results indicated ( 1 ) that CD45RC CD 4 T cells alone showed enhanced localization to the gut and PP , probably via alpha 4 beta 7 MAdCAM 1 interaction ; ( 2 ) that many CD45RC cells entered non mucosal LN independently of alpha 4 integrin or VCAM 1 ; and ( 3 ) that entry of mature recirculating CD45RC CD 4 T cells into the thymus across thymic endothelium was apparently regulated by alpha 4 integrin VCAM 1 interaction . . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
Reverse transcription polymerase chain reaction analyses revealed that PP CD 4 T cells expressed early Th 1 type ( interferon gamma and interleukin [ IL ] 2 ) and late Th 2 type ( IL 4 , 5 , and 6 ) cytokine mRNA . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
The CD 4 count was significantly higher in patients with PP . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
The CD 4 : CD 8 ratio in NALT cell preparations was less than that observed in PP and more closely resembled that seen in spleen . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
An age related decrease of the CD 4 subset was observed in SP of aged rats , but not in their PP . ^^^ When the maturation of the CD 4 T cells in PP was followed during the first week after weaning , we found that an important proportion of this subset changes its phenotype at this time , from recent thymic emigrant ( CD45RC Thy 1+ ) to naive T cell ( CD45RC+ Thy 1 ) and then to activated / memory cell ( CD45RC Thy 1 ) . ^^^ Therefore it appeared that CD 4 T cells from PP mature faster than SP CD 4 T cells , and they are not subject to the deleterious effect of aging . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
PP B cells from control animals also secreted elevated levels of IgA when cocultured with splenic CD 4 ( + ) cells from VT treated animals , thus confirming previous studies showing that T cell help also contributes to increased IgA production . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
We have immunized transgenic mice expressing the susceptible HLA DR ( alpha1 * 0101 , beta1 * 0401 ) ( hereafter called DRB1 * 0401 ) and human CD 4 molecules on a murine major histocompatibility complex class 2 null background , with human preproinsulin ( PPI ) , proinsulin ( PI ) , and insulin and derived large panels of T cell hybridomas to determine the immunogenic epitopes of these proteins . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
Therefore , PIP was performed for CD 4 , CD 8 , T cell intracellular antigen ( TIA ) 1 , and betaF 1 expression in 31 PTCLs previously studied for CD 4 and CD 8 by flow cytometry . ^^^ PIP showed 71 % correlation with the 21 PTCLs that had distinct CD4+ CD 8 or CD 4 CD8+ phenotypes by flow cytometry , with 64 % and 90 % sensitivity for CD 4 and CD 8 expression , respectively . ^^^ Tumor cells in four of six PTCLs that had no clear CD 4 or CD 8 predominance or coexpression of these antigens by flow cytometry were shown to be CD4+ CD 8 or CD 4 CD8+ by PIP . ^^^ Twelve ( 39 % ) PTCLs demonstrated a cytotoxic ( TIA 1+ ) phenotype by PIP , including eight CD 4 CD8+ , one CD4+ CD 8 and three CD 4 CD 8 cases . ^^^ We conclude that CD 4 and CD 8 T cell subsets can be assigned for most PTCLs by PIP , with CD 4 showing moderate and CD 8 showing strong correlation with flow cytometric results . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
PPTD was defined as both : an abnormal TSH , and fT 4 pp women developing PPTD and / or being positive for TPO Ab ( n = 26 ) ; and thyroidological uneventful control women of the same cohort , matched for age and parity ( n = 21 ) , were tested for thyroid stimulating antibodies , percentages of peripheral blood lymphocyte subsets using fluorescence activated cell sorter analysis ( CD 3 , CD 4 , CD 8 , CD 16 , CD 56 , major histocompatibility complex class 2 ) , for monocyte polarization , and for cluster capability of monocyte derived DC . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
We showed previously that blocking of only L selectin is sufficient to ablate trafficking of naive CD 4 cells and the development of their responses in peripheral lymph nodes but not in PP where alpha4beta7 integrins are thought to primarily regulate entry . ^^^ However , although antibody to alpha 4 integrins partially inhibited homing of naive CD 4 cells to PP and not to lymph nodes , there was no effect on the development primary responses in these tissues or spleens . ^^^ Since previous studies indicate that both alpha4beta7 integrins and L selectin regulate adhesion of naive cells to PP HEV , we examined the effect a blockade of both adhesion pathways on the recirculation of naive CD 4 cells . ^^^ There was no detectable homing of naive CD 4 cells to PP or lymph nodes when interactions with both receptors were inhibited , resulting in a profound depletion of naive CD 4 cells and loss of antigen responses in these sites . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
Taken together , the present results demonstrate that intestinal CD 4 Th1 cells from PP and MLN may be involved in the defence against Y . enterocolitica at different sites of the infection , and that PKH 2 GL labelling is a suitable tool to characterize T cell functions in vivo . . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
Also , phenotypic changes indicating germinal center reactions ( GCRs ) and the activation of CD 4 ( + ) T cells in PP were determined by using fluorescence activated cell sorter analyses . ^^^ Also , the proportion of CD 4 ( + ) , CD45RBlow T cells , indicative of activated cells , gradually increased in the PP to the level found in conventionally reared mice . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
Furthermore , antigen specific CD 4 ( + ) cells were found in PP . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
CD 4 ( + ) cells were distributed in the follicular medullas of the jejunal PP and the CT , but not in those of the ileal PP and the BF . ^^^ Because CD 4 ( + ) T cells would be prerequisite for Ig class switching in these follicles , IgG ( + ) cells of the follicular medullas in the ileal PP and the BF would trap immune complexes from the gut lumen . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
Current models suggest that inductive immune responses to enteric Ag are initiated in Peyer ' s patches ( PP ) and mesenteric lymph nodes ( MLN ) followed by migration of activated , memory like CD 4 ( + ) T cells to extralymphoid sites in the intestinal lamina propria ( LP ) . ^^^ Previously activated DO11 . 10 T cells in the LP responded to oral Ag earlier and at 50 % higher levels compared with memory CD 4 ( + ) T cells localized to PP tissue . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
Upon stimulation , only CD45RB ( low ) CD 4 ( + ) PP T cells produce IL 10 , whereas secretion of IL 2 , IL 4 , and IFN gamma was not detected . ^^^ We conclude that PP favor differentiation of an IL 10 producing , regulatory CD45RB ( low ) CD 4 ( + ) T cell population and that inhibition of T cell proliferation by activated PP MC may reflect regulatory activity consistent with T regulatory cells . . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
We have developed a model using transfer of DO11 . 10 TCR transgenic bone marrow into irradiated recipients in which it has been possible to track antigen specific CD 4 ( + ) cells in mesenteric lymph nodes ( mLN ) , Peyer ' s patches ( PP ) and lamina propria following primary exposure to antigen . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
In advanced stages of HIV disease ( CD 4 count < 50 cells / mm ( 3 ) ) , patients may develop opportunistic cytomegalovirus ( CMV ) nerve infection , which can present as progressive polyradiculopathy ( PP ) or mononeuropathy multiplex ( MM ) . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
The tonsils were similar to PP in the proportions of B and T cells ( 25 32 % T cells , 39 45 % B cells ) , and T cell subpopulations ( CD 4 , CD 8 , and gammadelta ) . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
Increased expression of CD 25 and the Th 1 associated chemokine receptor CCR 5 was also seen on CD 4 ( + ) and CD 8 ( + ) PP T cells , but not blood T cells , stimulated with betalg . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
Significant frequencies of donor CD 4 cells accumulated within recipient Peyer ' s patches ( PP ) , mesenteric lymph nodes ( MLN ) , lamina propria ( LP ) , and spleen ( SP ) , during the first 9 days of GVHR . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
L Sel ( / ) Peyer ' s patch ( PP ) CD 4 ( + ) Th cells revealed IFN gamma dominated responses and an unprecedented absence of IL 4 , whereas the expected mixed Th cell phenotype developed in L Sel ( + / + ) mice . ^^^ PP CD 4 ( + ) Th cell anti Salmonella responses were nearly nonexistent in L Sel ( / ) mice immunized with either Salmonella vaccine . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
Inhibitory effects mediated by CD 4 ( + ) PP cells were partially neutralized by antibodies to IL 10 and transforming growth factor beta . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
We show selective association of SIgA with dendritic cells and CD 4 ( + ) T and B lymphocytes recovered from PP in vitro . ^^^ In PP , SIgA associates with and is internalized by dendritic cells in the subepithelial dome region , whereas the interaction with CD 4 ( + ) T cells is limited to surface binding . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
RESULTS : CD 3 ( + ) T cells in NALT , NP and PP increased significantly on the 7th day after the immunization , in which most were CD 4 ( + ) T cells . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
Taken together , these results suggest that the novel subset of CD 4 ( ) c kit ( ) CD3epsilon ( ) IL 2Ralpha ( + ) PP cells are capable of secreting a high level of IL 5 in response to IL 2 , contribute markedly to IL 5 production and help IgA secretion by B cells . . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
RESULTS : CCL 20 was expressed in the FAE before birth at the time when the first hematopoietic CD 4 ( + ) CD 3 ( ) appeared in the PP anlage . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
However , these S IgA responses were supported by increased numbers of PP CD 4 ( + ) T helper ( Th ) cells secreting interleukin 5 ( IL 5 ) and IL 6 and splenic CD 4 ( + ) Th cells secreting IL 6 compared to NK1R ( + / + ) mice . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
Delivery of the vaccine by the oral route results in antigen specific CD 4 ( + ) and CD 8 ( + ) T cells in PP and spleen . ^^^ Addition of CT E29H results in an increase of antigen specific CD 4 ( + ) cell population in PP and both CD 4 ( + ) and CD 8 ( + ) populations in the spleen . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
This suppressive effect was also prominent in six BLG specific CD 4 ( + ) T cell clones ( OPP 1 6 ) established from PP from mice orally administered with high doses of BLG and was partially reversed by antibodies to TGF beta . ^^^ Thus , antigen specific CD 4 ( + ) CD 25 ( + ) T ( reg ) , which have characteristics of anergic cells and actively suppress antibody production are induced in PP upon oral administration of protein antigen . . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
PP were excised from mice , and lymphocyte subpopulations ( B lymphocytes , T lymphocytes , CD 4 ( + ) cells , and CD 8 ( + ) cells ) were determined by flow cytometry . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
Peyer ' s patch ( PP ) dendritic cells ( DCs ) have been shown to exhibit a distinct capacity to induce cytokine secretion from CD 4 ( + ) T cells compared with DCs in other lymphoid organs such as the spleen ( SP ) . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
Immunoperoxidase ( IPX ) labelling for CD 4 , CD 8 , TCR gammadelta , WC 1 , CD1b , IFN gamma , CD45R , CD 56 and lysozyme was used to investigate changes in cell mediated immune effector cell populations in the intestinal Peyer ' s patches ( PP ) and mesenteric lymph nodes of lambs , 2 and 4 months after experimental infection with low doses of sheep strain Mycobacterium avium subsp . paratuberculosis ( M . a . paratuberculosis ) . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
Based on the rates of formation of the specific excision products , ATP and PP ( 1 ) levels were determined : ATP was present at 1 . 3 to 2 . 2 mM in H 9 cells , macrophages , and unstimulated CD 4 ( + ) or CD 8 ( + ) T cells , while PP ( 1 ) was present at 7 to 15 microM . ^^^ Activated CD 4 ( + ) or CD 8 ( + ) T cells contained 1 . 4 to 2 . 7 mM ATP and 55 to 79 microM PP ( 1 ) . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
The cytokine measurement profile of IL 2 , IFN gamma and IL 6 and low levels of IL 4 in the cultural supernatants of SP , PP and LP showed mixed CD 4 ( + ) Th 1 and Th 2 immune response . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
Using a preproinsulin ( PPI ) peptide library approach and in vitro cytokine profiling , cellular autoimmunity was characterized in peripheral blood mononuclear cells ( PBMC ) and CD 4 ( + ) T helper cell subsets . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
CD 3 ( ) CD 4 ( + ) CD 45 ( + ) inducer cells are required for the initiation of mucosa associated organogenesis of both nasopharynx associated lymphoid tissues ( NALT ) and Peyer ' s patches ( PP ) in the aerodigestive tract . ^^^ CXCL 13 ( / ) mice and mice carrying the paucity of lymph node T cell ( plt ) mutation and lacking expression of CCL 19 and CCL 21 accumulate CD 3 ( ) CD 4 ( + ) CD 45 ( + ) cells at the site of NALT but not of PP genesis . ^^^
Interacting proteins: P12273 and P01730 Pubmed SVM Score :0.0
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