Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The data suggest that when anti CD 3 stimulation is suboptimal , responses can be enhanced by MAb to CD 5 or CD 28 ( Tp 44 ) or by immobilized MAb to CD 4 , CD 2 , CD11a ( LFA 1 ) , or class 1 MHC encoded gene products . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Anti CD 4 and CD 8 antibodies inhibit alternative , nonreceptor pathways of T cell triggering via Tp 103 and Tp 44 in the absence of class 2 positive accessory or target cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Antibodies binding to a large subset of T cell differentiation antigens , including CD 2 , CD 4 , CD 5 , CD 6 , CD 7 , CD 8 , Tp 44 , and CDw 18 , cause an increase in the cytoplasmic calcium concentration [ ( Ca2+ ] 1 ) after the antigens are crosslinked on the cell surface . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
We investigated the phosphorylation of CD 2 , CD 3 , CD 4 , CD 5 , CD 7 , CD 8 , CD 28 ( Tp 44 ) , CD 43 ( sialophorin , gp 115 ) , and LFA 1 after incubation of human PBMC with the ( PKC ) activator PMA . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
With Tp 44 and CD 11 , and CD 4 and CD 8 , the development and function of T cells can thus be examined within the framework of two distinct systems of reciprocally expressed antigens . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Whereas Nef can reduce expression of CD 4 , Tat reduces the expression of CD 28 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The levels of expression of cell surface molecules involved in T cell functions ( CD 4 , CD 3 , CD 28 , CD 29 , IL 2 receptor ) were not modified in cell populations expressing Nef . ^^^ In immunocompetent T cell clones , cell proliferation and lymphokine production in response to activating stimuli ( IL 2 , alloantigens , phorbol esters , or antibodies directed against CD 2 , CD 3 , CD 4 , CD 28 ) remained unmodified . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
T cell antigens including CD 2 , CD 4 , CD 6 , CD 8 , and CD 28 serve as coreceptors with the T cell receptor ( TCR ) / CD3 complex in control of T cell growth . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
CD 28 expression among CD 4 CD8+ and CD 4 CD8 was variable but lower than on CD4+ T cell clones . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Signal transduction via CD 4 , CD 8 , and CD 28 in mature and immature thymocytes . ^^^ Subsequent cell proliferation could be induced by soluble anti CD 28 mAb , which was comitogenic for cells stimulated with CD 3 10 CD 4 or CD 3 10 CD 8 cross linking , but was without effect on cells stimulated with CD 3 10 CD 3 cross linking . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Antigen specific nonresponsiveness was induced by both nonmitogenic and mitogenic anti CD 3 antibodies but not by antibodies against CD 2 , CD 4 , CD 5 , CD 8 , CD 18 , or CD 28 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
No differences in expression of the CD 2 , CD 3 , CD 4 , Tp 103 , and CD 28 Ag between these cells and the parental cells could be demonstrated , with the exception of the expression of IL R p 55 and HLA DR Ag , which were constitutively expressed on the phase 1 cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Virtually all TCR gamma / delta+ thymocytes / lymphocytes expressed CD 7 , CD 2 , and CD 5 but were heterogeneous with respect to their expression of CD 1 , CD 4 , CD 8 , CD 28 , CD11b , CD 16 , and Leu 7 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In addition to the CD 3 T cell receptor complex , the CD 2 , CD 4 , CD 5 , CD 7 , CD 8 and CD 28 receptors mobilize cytoplasmic calcium within minutes of binding with monoclonal antibodies and additional crosslinking occurs on the cell surface . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Purified CD 4 T cells that do not respond to CD 3 mAb in solution do respond to the CD3 / CD4 heteroconjugate in solution by proliferating in the presence of a CD 28 mAb , with a significant fraction of CD 4 cells entering the second cycle within the first three days of stimulation . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
All of the five clones selected for detailed analysis did not express CD 4 , CD 8 , and CD 28 antigens and did not release IL 2 , whereas they displayed cytolytic activity against NK sensitive , NK resistant , and fresh tumor target cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Ligation of CD 28 on CD 4 Th1 clones and freshly isolated mixtures of naive and memory CD 4 T cells triggered their T cell receptors ( TCR ) is sufficient to induce the costimulatory signals necessary for interleukin 2 ( IL 2 ) production by these cells . ^^^ Because B cell blasts express CTLA 4 reactive ligands and high levels of adhesion and major histocompatibility complex class 2 molecules , they would be expected to engage both the TCR and CD 28 and consequently stimulate IL 2 production by naive CD 4 T cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
We conclude that CD 28 ligation with the natural ligand B 7 1 provides a strong accessory signal for CD 4 and CD 8 cell activation through CD2 . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
DC mediated transmission was blocked by MAb to CD 4 and MHC class 2 , but strongly increased by MAb to CD 40 on DCs or CD 28 on T cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In the absence of gp 120 , CD 4 displayed high association with CD 3 , CD 5 , CD45RC , CD 25 , CD 28 , CD 44 , and CD 53 ; weak association with CD 2 , CD 38 , CD45RB , CD62L , and CD 26 ; and no association with CD45RA , CD45RO , CD11b , CD11a , CD 54 , CD 7 , CD 48 , CD 98 , CD 59 CD55 , HLA class 1 and class 2 molecules . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Here , we demonstrate that ligation of CD 2 and CD 28 in conjunction with TCR occupancy rescue T cells that have been programmed for apoptotic death by prior CD 4 ligation to gp 120 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The relative density of lymphocyte CD 3 , CD 4 , CD 5 , CD 8 , CD 20 , CD 23 , CD 28 , CD 38 , CD45RA , CD45RO , CD 57 and HLA DR antigens was measured as antibody binding capacity ( ABC ) in 58 blood donors aged 19 66 years . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Tyr phosphorylation was observed when the T cells were activated by crosslinking CD 3 or by cocrosslinking CD 3 with CD 2 or CD 4 , but not when the cells were stimulated by crosslinking CD 2 , CD 4 , or CD 28 alone . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
CTL activity could be generated by CD 3 and CD 28 ligation in both CD 4 ( + ) and CD 8 ( + ) subpopulations . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Activities found in four lots each of horse ATG ( ATGAM , Upjohn ) , rabbit and horse ATG ( thymoglobulin and lymphoglobulin , Merieux ) , horse ALG ( Minnesota ) , and rabbit ATG ( Fresenius ) have been compared for CD 2 , CD 3 , CD 4 , CD 5 , CD 7 , CD 8 , CD11a , CD 18 , CD 28 , CD 44 , CD 45 , and TCR alpha / beta antigens . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Milk T cells had a more equivalent CD 4 : CD 8 ratio than blood ; milk CD 4 T cells mainly expressed the CD45RO ( antigen primed / memory ) phenotype ; milk CD 8 cells had an equivalent CD11b : CD 28 suppressor : cytotoxic phenotype ; and milk T cells had 2 3 fold higher percentages of activated CD 4 IL 2R and CD 8 HML 1 or CD 8 VLA 1 cells than blood . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
These results indicate that the costimulatory signal delivered by CD 28 may have fundamentally different biochemical properties in CD 4 and CD 8 T cell subsets , and therefore the functional role of CD 28 may differ in these T cell subsets . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Single , two and three colour flow cytometry was performed using FITC labelled MoAbs directed against the following TCR 5 beta subfamilies : V 2 , V 3 , V5a , V5b , V5c , V 6 , V 8 , V 12 , V13 . 3 , V 17 , V 19 , alone or in combination with PE labelled CD 4 , CD 8 , HLA DR , CD 28 , CD 38 and with Peridinin chlorophyll A protein ( PerCP ) conjugated CD 8 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The major costimulatory molecules for CD 4 T cells appear to be B 7 and B7 . 2 that bind to the CD 28 and CTLA 4 receptors on the T cell . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
CD 2 , CD 4 , CD 28 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
As tyrosine phosphorylation is the earliest of the biochemical events initiated by stimulation of CD 3 TCR , we studied protein tyrosine phosphorylation in purified CD4+ lymphocytes from 25 asymptomatic seropositive patients ( CD 4 T cells > 350 / mm3 ) previously stimulated in vitro by immobilized anti CD 3 mAb or by co immobilized anti CD 3 and anti CD 28 mAbs . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The proliferation of CD 8 T cells is inhibited by mAbs reactive with CD 8 or HLA A and B molecules but not with CD 4 or HLA DR . mAb blocking studies show a role for CD 2 , LFA 3 , and CD 59 , but not for intercellular adhesion molecule 1 , intercellular adhesion molecule 2 , very late activation Ag 4 , vascular cell adhesion molecule 1 , CD 28 , or CD 28 ligand , as costimulatory molecules . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Two step TCR zeta / CD3 CD 4 and CD 28 signaling in T cells : SH2 / SH3 domains , protein tyrosine and lipid kinases . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Whereas some TcR alpha beta expressing IEL subsets such as CD4+8 and CD 4 8 alpha+ beta+ cells express CD 28 at the same levels as their phenotypic counterparts in lymph node , other subsets of TcR alpha beta cells ( including CD 4 8 alpha+ beta and CD4+8 alpha+ beta cells ) as well as TcR gamma delta+ IEL fail to express CD 28 . ^^^ These results suggest that CD 28 IEL ( CD 4 8 alpha+ beta cells , and some CD4+ 8 alpha+ beta cells ) may follow a different developmental pathway from that of CD28+ IEL in a thymus independent environment , and that expression of CD 28 correlates with responsiveness of the cells to triggering via the TcR CD 3 complex . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Crosslinking CD 28 causes a shift in the relative mobility of p56lck from 56 to 60 kD similar to that seen after crosslinking of CD 2 and CD 4 , cell surface receptors known to be associate with and activate p56lck . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
None of the other cytokines tested ( IL 1 alpha , IL 6 , TNF alpha , IFN alpha , IFN gamma ) supported growth of NK 92 cells which have the following characteristics : surface marker positive for CD 2 , CD 7 , CD11a , CD 28 , CD 45 , CD 54 , CD56bright ; surface marker negative for CD 1 , CD 3 , CD 4 , CD 5 , CD 8 , CD 10 , CD 14 , CD 16 , CD 19 , CD 20 , CD 23 , CD 34 , HLA DR . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Resting T lymphocytes that have recognized antigen bound to a major histocompatibility complex molecule with the T cell receptor require costimulatory signals through accessory receptors , including CD 2 , CD 4 , CD 8 , and CD 28 , for their clonal growth and expression of their functional repertoires . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
At these low doses of bound SEA , monoclonal antibodies to CD 3 , CD 4 , and CD 28 almost completely block T cell proliferation . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In this study we have investigated the putative role of D 3 phosphoinositides as mediators of CD 28 receptor signaling , since phosphoinositide ( PI ) 3 kinase , the enzyme responsible for D 3 phosphoinositide formation , is a known substrate for protein tyrosine kinases associated with certain T cell surface receptors such as CD 4 and interleukin 2 receptor . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Using a sensitive immune complex kinase reaction , we show that cross linking of the stimulatory and costimulatory receptors CD 3 , CD 4 , or CD 28 induces c Raf 1 activation in highly purified resting peripheral blood human T cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Protein tyrosine kinases have been implicated in signal transduction in T lymphocytes after stimulation of many cell surface molecules , including the T cell antigen receptor , CD 4 , CD 8 , CD 2 , CD 5 , and CD 28 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Various T cell accessory molecules including CD 4 , CD 28 , and LFA 1 participate and facilitate the activation event . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Both CD4+ CD8+ subsets resembled CD4+ CD 8 cells in CD45RA , CD45RO , and CD 25 expression ; the comparable CD CD8+ cells in CD 62 L expression ; and CD 4 CD8bright cells in CD11b , CD11b , CD16 / 56 , and CD 28 expression . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
We demonstrate in this report that both CD 28 B7 . 1 and CD 28 B7 . 2 interactions induce the association of PI 3 K to CD 28 in the CD 4 subpopulation , whereas it was barely detectable in CD 8 cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
CD 4 T cells isolated from the CD 28 deficient mice can receive costimulatory activity from CD40L induced B cells , demonstrating that the induced molecules can costimulate T cells by a CD 28 independent mechanism . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Upregulation of IL 2 gene expression in CD 4 T cells by CD 28 co stimulation increases the amount of IL 2 mRNA per cell as visualized by mean fluorescence intensity . ^^^ In addition the effect of CD 28 co stimulation on IL 2 mRNA stabilisation was demonstrated by the maintenance of a high frequency of IL 2 expressing CD 4 T cells and an elevated level of mRNA per cell for prolonged period after PMA+Io stimulation . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NKL cells express CD 2 , CD 6 , CD11a , CD 26 , CD 27 , CD 29 , CD 38 , CD 43 , CD 58 , CD 81 , CD 94 , CD 95 , class 2 MHC , and the C1 . 7 . 1 antigen , but do not express detectable levels of CD 3 , CD 4 , CD 5 , CD 8 , CD 14 , CD 19 , CD 20 , CD 28 , alpha / beta or gamma / delta T cell receptors on the cell surface . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In this report we have demonstrated that the inability of MAIDS CD4+ T cells to respond to CD 3 stimulation was not associated with reduced surface expression of CD 3 , CD 4 , or CD 28 and could not be overcome by costimulation with anti CD 28 antibody . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
CD1a+ cells differed from CD14+ cells by ( 1 ) dendritic morphology , ( 2 ) higher expression of CD1a , CD1c , CD 4 , CD 40 , adhesion molecules ( CD11c , CD 54 , CD 58 ) , major histocompatibility complex ( MHC ) class 2 molecules and CD 28 ligands ( CD 80 and CD 86 ) , ( 3 ) lack of Fc receptor FcgammaRI ( CD 64 ) and complement receptor CR 1 ( CD 35 ) expression , and ( 4 ) stronger induction of allogeneic T cell proliferation . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Immunophenotyping of lymphoma revealed CD45++ , c kit dim+ , terminal deoxynucleotidyl transferase ( TdT ) < + , CD38++ , CD34+ < ++ , CD33+ < , CD13dim+approximately+ , HLA DR+ , CD7+ , cytoplasmic CD 3 ( cCD 3 ) + , surface CD 3 ( sCD 3 ) , CD2dim+ , CD56+ , CD 16 , CD11b+ , CD 57 , CD1a , CD 5 , TCR alpha beta , TCR gamma delta , CD 4 , CD 8 , CD 28 , CD 10 , CD 19 , CD 20 , CD 22 , surface immunoglobulins , and CD 14 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
CD 28 , investigated in CD 3 , CD 4 and CD 8 positive T cells from 18 CLL patients appeared to be reduced in the three subsets but more marked in CD 8 positive lymphocytes . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Many cell signals such as CD 28 and CD 4 binding can costimulate cytokine gene expression in activated T cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Using a selected anti CD 3 mAb ( 64 . 1 ) we next demonstrate that colligation of CD 3 and CD 28 with soluble mAb is sufficient to activate highly purified naive CD 4 T cells for proliferation , IL 4 mRNA expression , IL 4 secretion , and maturation into IL 4 and IL 5 producing cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Although Abs specific for CD 4 , CD 26 , CD 28 , CD 44 , CD45RA , or CD45RO were sufficient to activate T cells when co immobilized with anti CD 3 mAb , all were refractory to the inhibitory effects of mAb 18D3 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Therefore , in order to elucidate further the pathogenesis of psoriasis we studied the expression of CD 27 and CD 28 , together with CD 4 , CD 8 and CD45RA in this benign inflammatory dermatological disease . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Here , we show that interleukin ( IL ) 12 and IL 4 directly induce human neonatal CD 4 T cells , activated via CD 3 and CD 28 , to differentiate into Th 1 and Th 2 subsets . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Some of these TCR antagonistic analogues carrying relatively conservative amino acid substitutions partially activated the T cells to induce large increases in size and expression levels of CD 4 , CD11a ( LFA 1 ) , CD 28 , CD49d ( VLA 4 ) , and CD 95 ( Fas ) , and small increases in CD 25 and CD 44 expressions on the cell surface . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The lack of CD 28 expression is a common denominator of CD4+ , CD8+ , and CD 4 CD 8 T cells prone to develop clonal dominance . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
CD 4 , LFA 1 ( CD11a / CD18 ) , and CD 28 were critical in the adhesion response . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Our extensive phenotype analysis of peripheral blood lymphocytes ( PBL ) from SLE patients revealed that the absolute number of CD28+ T cells of both CD 4 and CD 8 phenotypes was selectively decreased , while that of CD 28 T cells was maintained . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
A two colour cytofluorometry was employed using monoclonal antibodies against Fas receptor ( CD 95 ) in combination with the surface markers CD 4 , CD 8 , CD 28 , CD 26 and CD45RO . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
We have used this method to generate functional sFv to human CD 2 , CD 3 , CD 4 , CD 8 , CD 28 , CD 40 , CD 45 and to murine CD 3 and gp 39 , from hybridomas producing murine , rat , or hamster antibodies . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Multiparameter FACS analyses of peripheral blood mononuclear cells were performed using antibodies against CD 3 , CD 4 , CD 8 , HLA DR , CD 56 , CD 28 , CD 38 , CD 19 , sIgM , and sIgG . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Chimeric proteins in which the CD 2 transmembrane and / or cytoplasmic domains were deleted or exchanged with analogous regions of CD 4 , CD 28 or CD 58 retained association with high levels of murine CD 45 phosphatase activity , suggesting that the CD 2 extracellular domain largely controls interaction with CD 45 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Thus , exposure of T cells to physiological concentrations of STE or nicotine can alter the T cell expression of CD 28 and CTLA 4 , and the CD 4 T cell cytokine expression pattern . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
However , CD2+ DC precursors alone gave rise to APC that presented soluble Ags to naive CD4+ T cells , a property that could be inhibited by Abs to CD 4 , CD11a , and CD 28 on T cells or CD 86 on DC . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Collaboration of TCR , CD 4 and CD 28 mediated signalling in antigen specific MHC class 2 restricted T cells . ^^^ A previously developed experimental system was applied to obtain qualitative and quantitative data on the contribution of TCR , CD 4 and CD 28 mediated signalling in the activation of an antigen specific T cell hybridoma . ^^^ These results demonstrate that distinct peptide doses and the presence or absence of CD 4 result in quantitative changes in T cell responses , while the degree of CD 28 mediated signalling has a qualitative affect on the outcome of T cell activation , revealed by complete or partial inhibition of IL 2 secretion as a result of limited CD 28 B7 interaction as well as by alteration in the duration and time kinetics of the calcium response . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Cells were analyzed from the arterial line before , at 5 , 10 , 15 , 30 , 90 and 240 min with a panel of 33 surface markers including antibodies towards CD 3 , CD 4 , TCR alpha / beta , CD 7 , CD 8 , CD11a c , CD 14 , CD 18 , CD 19 , CD 25 , CD 28 , CD 29 , CD 38 , CD 44 , CD49a e , CD51 / 61 , CD 54 , CD 56 , CD 58 , CD 62 , CD 106 , and HLA class 2 using single fluorescence analysis . ^^^ Our results suggest a substantial difference in the expression of various surface markers and cell adhesion molecules ( lymphocytes : CD 4 , CD 25 , CD 28 , CD 51 , CD 54 ; monocytes : CD 49 , CD 54 , CD 58 , CD62E , CD62P ) compared to healthy persons , and a distinct modulation during hemodialysis ( lymphocytes : CD 11 , CD 18 , CD 49 , CD 51 , CD 61 ; monocytes : CD 29 , CD 49 , CD 51 , CD 54 , CD 61 ) which might affect the immune response beyond the single dialysis session . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Activation of CD 4 ( + ) T lymphocytes from human immunodeficiency virus type 1 ( HIV 1 ) infected donors with immobilized antibodies to CD 3 and CD 28 induces a virus resistant state . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In this report , we demonstrate that the T cell tropic strain of HIV , LAI , does not replicate in naive CD 4 T cells stimulated by cross linking CD 3 and CD 28 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The levels of expression of the CD 2 , CD 3 , CD 4 and CD 28 antigens were similar ; however , CD 45 and CD45RA antigen expression were slightly lower in cord cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Induction of HIV 1 resistance was specific for CD 28 costimulation , in that a variety of other accessory receptors , such as CD 2 , CD 4 , CD 5 , and MHC class 1 , failed to confer the antiviral resistance . ^^^ The soluble component was secreted by both CD 4 and CD 8 T cells , was not unique to CD 28 costimulation , and could be neutralized by removal of C C chemokines ( RANTES ( regulated upon activation , normal T cell expressed and secreted ) and macrophage inflammatory protein 1alpha and 1beta ) from the culture supernatants of costimulated CD 4 T cells . ^^^ In contrast , CD 28 stimulation of CD 4 cells resulted in the specific induction of a pronounced intrinsic resistance to HIV 1 infection by macrophage tropic isolates of HIV 1 . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Naive CD 4 cells stimulated with high concentrations of antigen and without CD 28 costimulation produced low but detectable amounts of IL 2 and interferon gamma . ^^^ Furthermore , naive CD 4 T cells activated for a 7 day period by either low or high concentrations of antigen with or without CD 28 costimulation were functionally similar , indicating that signals transmitted through the TCR were not intrinsically tolerogenic for CD 4 T cells . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
CD 4 T cell anergy in murine AIDS : costimulation via CD 28 and the addition of IL 12 are not sufficient to rescue anergic CD 4 T cells . ^^^ We examined responses of anergic CD 4 T cells upon addition of a costimulatory signal ( anti CD 28 ) and a cytokine ( IL 12 ) , which might help to restore the function of cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The effects of CD 3 , CD 4 and CD 28 signaling on lymphocytes during human immunodeficiency virus 1 infection . ^^^ We investigated the effects of early human immunodeficiency virus 1 infection ( HIV 1 ) on CD 4 and CD 28 mediated co signaling of the T cell receptor ( TCR ) / CD3 complex in peripheral blood lymphocytes ( PBL ) . ^^^ Our data demonstrate the importance of appropriate CD 3 , CD 4 and CD 28 co stimulatory function to prevent apoptosis . ^^^ The CD 4 mediated signaling defects of the TCR could contribute to the loss of immunocompetent cells during HIV 1 infection via activation induced cell death , whereas stimulation through the CD 28 pathway could reverse these detrimental effects . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
CD 28 and CTLA 4 mRNA were detected in efferent lymph and afferent lymph populations containing CD 4 and CD 8 T cells stimulated with Con A and IL 2 or with Con A alone , respectively . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Immunochemistry was performed on fresh tissue using a panel of monoclonal antibodies including anti CD1a , CD 3 , CD 4 , CD 8 , CD 16 , CD 20 , CD 28 , and CD 68 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
After depletion of CD 8 cells from the peripheral blood mononuclear cells ( PBMCs ) of both patients and normal donors , activation of patient CD 4 lymphocytes with immobilized antibodies to CD 3 and CD 28 enabled the isolation of virus from PBMCs of six patients despite the suppression of their plasma HIV RNA to fewer than 50 copies per milliliter for up to 2 years . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Impaired induction of the CD 28 responsive complex in granulocyte colony stimulating factor mobilized CD 4 T cells . ^^^ Furthermore , as shown in electrophoretic mobility shift assays , the inducible level of the T cell transcription factor CD 28 responsive complex ( CD28RC ) was suppressed in CD 4 cells derived from G PBMC . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
We now report that CD 28 derived costimulation can revert the nonresponsive state of antigen exposed CD 4 T cells . ^^^ We therefore examined the possible role of Bcl 2 family proteins in the nonresponsiveness of antigen exposed CD 4 T cells , and determined whether CTLA 4 , in analogy to CD 28 , mediates its negative regulatory effects via the Bcl 2 family of apoptotic mediators . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
However , targeted mutation of CD 28 does not significantly affect production of IgG and activation of CD 4 T helper cells in response to infections by some viruses and nematode parasites . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
To determine whether the strength of TCR signaling controls the involvement of CD 28 costimulation in selective CD 4 T cell differentiation , naive CD 4 T cells bearing a transgenic TCR are primed by a weak or strong TCR signal ( signal 1 ) in the presence or absence of B 7 costimulatory molecules ( signal 2 ) . ^^^ Thus , our results suggest that naive CD 4 T cells are receptive to CD 28 dependent IL 4 production only if they receive a weak TCR signal . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Expression of the costimulatory receptor CD 28 in CD 8 cells was significantly reduced , and the apoptosis repressor ratio of bcl 2 / bax in both CD 4 and CD 8 was increased in patients ( P < 0 . 05 ) . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Notably , a similar pattern of increased CD3+ CD57+ , CD3+ HLA DR+ , CD3+ CD ( 16 + 56 ) + , CD 4 CD8+ , CD8+ CD57+ , CD8+ CD 28 , and CD8+ CD62L subsets was detected . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Cohorts of 97 centenarians , 40 subjects aged 70 90 ( ELD group ) , and 40 young adults ( under age 40 ) were phenotyped for T cell surface expression of CD 28 , CD 4 , and CD 8 antigens . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
However , the inhibitory effects of YHB . 46 on T cell proliferation were equipotent in the presence or the absence of CTLA 4Ig , showing that the CD 4 mAb was not acting on CD 28 induced activation signals per se . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
For example , binding of antibodies to the CD 28 coreceptor can downregulate expression of beta chemokine receptors , and this appears to have important consequences on the susceptibility of CD 4 ( + ) T lymphocytes to infection by HIV 1 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
We report here an infant with clinical and laboratory features of SCID and selective CD 4 lymphopenia and lack of CD 28 expression on CD 8 ( + ) T cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
T lymphocyte populations , including CD 3 , CD 4 , CD 28 , and adhesion molecules ( L selectin , LFA 1 , VLA 4 , and ICAM 1 ) were measured by flow cytometry in a cross sectional study of 100 HIV infected and 49 HIV seronegative adults . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
MTX resistance of Jurkat T cells in vitro was accompanied by significantly ( P < 0 . 05 ) decreased expression of CD 2 , CD 3 , CD 4 , CD 28 , and CD 69 , IL 2 production , and MTX uptake assessed by cell association or disassociation of 3 [ H ] MTX or fluoresceinated MTX ( FMTX ) , respectively . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In the CD 4 population , mean CD 28 and CD 95 expression was not altered in category 1 patients . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
A decrease of the percentage of CD 4 ( + ) CD 28 ( + ) cells and an increase in the expression of HLA DR both on CD 4 ( + ) and on CD 8 ( + ) cells suggest that although these T cells express activation markers their function may be altered by the lack of CD 28 expression . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Here we describe that aging is associated with the emergence of an unusual CD 4 T cell subset characterized by the loss of CD 28 expression . ^^^ The loss of CD 28 expression is correlated with a lack of CD 40 ligand expression rendering these CD 4 T cells incapable of promoting B cell differentiation and immunoglobulin secretion . ^^^ We propose that the emergence of CD 28 deficient CD 4 T cells in the elderly can partially explain age specific aberrations in immune responsiveness . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The decreasing levels of CD 4 ( + ) cells were characterized by an increasing frequency of cells expressing the activation markers HLA Dr and CD45RO but not the CD 28 surface antigen . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Nave and memory CD 4 T cells differ in their susceptibilities to human immunodeficiency virus type 1 infection following CD 28 costimulation : implicatip6s for transmission and pathogenesis . ^^^ To explore this paradox , we stimulated CD45RA+ CD 4 ( + ) ( nave ) and CD45RO+ CD 4 ( + ) ( memory ) cells with antibodies to CD 3 and CD 28 and infected them with either CCR 5 dependent ( R 5 ) or CXCR 4 dependent ( X 4 ) HIV 1 isolates . ^^^ While both nave and memory CD 4 ( + ) subsets downregulated CCR 5 expression in response to CD 28 costimulation , only the memory cells produced high levels of the beta chemokines RANTES , MIP 1alpha , and MIP 1beta upon stimulation . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Reverse transcription PCR ( RT PCR ) , competitive RT PCR , and Northern blot demonstrated that the expression of MHC class 2 group genes [ MHC class 2 , MHC class 2 associated invariant chain ( 2 ) , CD 4 and IgM ( B cell marker ) ] , MHC class 1 group genes ( MHC class 1 and CD 8 ) , B 7 1 ( costimulator on dendritic cells ) , and CD 28 ( receptor to B 7 on T cells ) in the pyloric mucosa was elevated by MNNG in both rat strains but was elevated to a 4 7 fold greater extent in Buffalo rats than in ACI rats . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Age related persistent clonal expansions of CD 28 ( ) cells : phenotypic and molecular TCR analysis reveals both CD 4 ( + ) and CD 4 ( + ) CD 8 ( + ) cells with identical CDR 3 sequences . ^^^ As indicated by FACS analysis , the clonally expanded cells occur in the CD 4 ( + ) CD 28 ( ) cells . ^^^ Taken together , these results suggest that expanded CD 4 ( + ) CD 28 ( ) cells might also acquire CD 8 alpha / alpha expression and become DP and imply that CD 4 clonality is a more frequent phenomenon than previously suspected . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Two million CD 4 ( + ) CD 28 ( / ) or CD 28 ( + / + ) T cells were transplanted into sublethally irradiated ( 750 cGy ) , MHC class 2 incompatible ( B 6 10 bm 12 ) F 1 recipients . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
We previously reported that anti CD 3 activated and CD 28 costimulated naive human CD 4 ( + ) T cells themselves release very low but sufficient levels of IL 4 to support their development into high IL 4 producing cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In the stepwise forward multivariate logistic regression models , after adjustment for CD 4 T cell counts , depending on whether it was analyzed continuously or after dichotomization ( 20th percentile ) , both T cell reactivity to CD 2 plus CD 28 antibodies or to CD 3 antibodies were the most predictive markers of DTHA ( odds ratio , 0 . 80 ; 95 % confidence interval , 0 . 67 0 . 94 ; and odds ratio , 2 . 97 ; 95 % confidence interval , 1 . 1 8 . 3 , respectively ) . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
CTLA 4 , through TGF beta , may serve as a counterbalance for CD 28 costimulation of IL 2 and CD 4 ( + ) T cell activation . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In order to evaluate immune response during pollen exposition cellular populations CD 2 , CD 4 , CD 8 , CD 19 , CD 22 , CD 23 , CD 28 , CD 29 , CD45RA , CD45RO have been studied before , during and after pollen season by flux cytometry . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Furthermore , expression of CD1a , CD 4 , CD 8 , CD 28 , CD 54 , CD 80 , CD 86 and HLA DR on migrating lymph cells and cytokine levels ( IL 1alpha , IL 1beta , IL 2 , IL 6 , IL 8 , IL 10 , IL 13 , TNF alpha and IFN gamma ) in the afferent lymph were analyzed by cytofluorometry and ELISA . ^^^ However , no changes in the expression of CD1a , CD 4 , CD 8 , CD 28 , CD 54 , CD 80 , CD 86 and HLA DR on migrating lymph cells were detectable . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
CD 28 expression was present on most CD 4 cells but was lower on CD 8 cells especially from those patients who also showed evidence of expanded T cell clones ( median 40 % . z=2 . 4 ; p < 0 . 02 ) . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Resting T cells treated with sub lethal levels of BPD MA and light did not exhibit changes in surface levels of CD 3 , CD 4 , CD 8 , CD 28 , CD 45 or T cell receptor ( TCR ) beta chain structures . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
As the dietary DHA concentration increased , the surface expression of CD 4 and CD 8 on splenic T cells decreased , while that of CD 28 increased . ^^^ These observations suggest that diets rich in DHA exert some of their immunomodulatory effects by a downregulation of surface expression of CD 4 and CD 8 and by an upregulation of CD 28 mediated costimulatory signal . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In both age groups , there was a strong association with CMV seropositivity and increased number of CD 28 CD 4 or CD 8 T cells , as well as with increased numbers of T cells expressing CD 56 or DR . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Prevalent class 1 restricted T cell response to the Theiler ' s virus epitope Db : VP 2121 130 in the absence of endogenous CD 4 help , tumor necrosis factor alpha , gamma interferon , perforin , or costimulation through CD 28 . ^^^ H 2 ( b ) mice lacking CD 4 , class 2 , gamma interferon , or CD 28 expression are susceptible to persistent virus infection but surprisingly still generate high frequencies of CD 8 ( + ) , Db : VP 2121 130 specific T cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Finally , we characterized two enzymes , PI3Kinase and PI PLC , which are both downstream effectors of the CD 4 ( HIV envelope protein receptor ) and CD 28 ( B 7 receptor ) activation pathways , and that participated in the early steps of priming for apoptosis . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Analyses included a flow cytometric immunophenotyping of peripheral blood mononuclear cells ( CD 3 , CD 4 , CD 8 , CD 19 , CD 25 , CD 28 , CD 56 , CD 57 , CD 80 and HLA . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
BR mice reflected quantitative or qualitative changes in T helper cells was examined by comparing cytokine production and expression of cell surface markers ( CD 4 , CD 8 , and CD 28 ) in mesenteric lymph node cells and spleen cells from both strains and comparing these with the characteristics of cells from resistant CBA mice and from CBA mice that had been rendered permissive to infection by a combination of irradiation and corticosteroid treatment . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In this study we have investigated the kinetics of CD 28 and CTLA 4 expression upon CD 4 ( + ) T cell activation in response to soluble peptide in vivo . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
METHODS : The proportion of CD 8 T cells that stain for CD 38 and HLA DR , CD 28 and CD 57 was compared with plasma viraemia and CD 4 cell count . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Nave and memory CD 4 T cells , CD 28 expression on CD 4 and CD 8 T cells , and restoration of in vitro proliferative response to mitogens and recall antigens increased in the intravenous and subcutaneous groups . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
CD 28 is the primary co stimulatory receptor for inducing high level IL 2 production and survival of nave CD 4 ( + ) T cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
This overexpression of CD 80 resulted in the loss of detectable cell surface CD 28 expression on thymocytes and a significant reduction in both the surface T cell receptor expression and the ratio of CD 4 ( + ) to CD 8 ( + ) single positive thymocytes in Tg animals compared to nontransgenic ( non Tg ) controls . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
A small fraction of rat 13 day old fetal liver ( r13FL ) cells , which weakly expressed the leukocyte common antigen CD 45 , constituted a homogeneous Thy 1 ( hi ) , CD 71 ( ) , CD 44 ( + ) , MHC class I+ , CD 43 ( + ) cell subpopulation negative for CD45RC , CD 3 , TCRalphabeta , TCRgammadelta , CD 2 , CD 5 , CD 4 , CD 8 , CD 25 , CD 28 , NKR P1a and sIg . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Costimulation with anti CD 28 overcame the inhibitory effect of anti CD 4 Abs , by induction of JNK activation . ^^^ Furthermore , animals pretreated with anti CD 4 elicited enhanced IL 4 secretion induced by OVA and CD 28 . ^^^ These observations suggest that CD 4 specific Abs can inhibit T cell activation by interfering with signal 1 transduced through the TCR , but potentiate those delivered through the costimulatory molecule CD 28 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
HIV specific cytotoxic T lymphocyte precursors exist in a CD 28 CD8+ T cell subset and increase with loss of CD 4 T cells . ^^^ More CD 28 positive HIV specific cytotoxic T lymphocyte precursors were found in patients with CD 4 : CD 8 ratios > 1 , whereas more CD 28 CD57+ precursors were found in patients whose CD 4 : CD 8 ratios were < 1 ( r 2 , 0 . 68 ) . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Although the majority of repopulating CD 4 ( + ) T cells in the intestinal mucosa were functionally capable of providing B and T cell help , as evidenced by their expression of CD 28 , these CD 4 ( + ) T cells were found to have a reduced capacity to produce interleukin 2 ( IL 2 ) compared to the potential of CD 4 ( + ) T cells prior to SIV infection . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In order to investigate the possibility of whether or not the lymphocytes of patients with Alzheimer ' s Disease ( AD ) are in an activated state , blood mononuclear cells from 45 AD patients and 45 healthy age matched controls were immunophenotyped by measuring the expression of CD 3 , CD 4 , CD 7 , CD 8 , CD 25 , CD 28 , CD 56 and HLA DR by flow cytometry . ^^^ A significant increase in the CD 4 , CD 25 and CD 28 antigen expression was also observed in the AD group ( 55 . 3 % 24 . 8 % and 65 . 1 % ) with respect to healthy subjects ( 44 . 5 % , 10 . 3 % and 54 . 3 % ) . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
TCR alpha beta independent CD 28 signaling and costimulation require non CD 4 associated Lck . ^^^ We demonstrate that CD 4 , but not CD4+ clonal variants respond to CD 28 specific mAb with both early and late indicators of activation . ^^^ Forced expression of A418 / A420 mutated CD 4 or wild type CD 4 in the CD 4 variant recapitulated the CD 28 mediated responses of the CD 4 and CD4+ variants , respectively . ^^^ The results demonstrate that constitutive levels of non CD 4 associated Lck functionally limit CD 28 mediated signaling . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Expression of cell surface CD 3 , CD 4 and CD 28 was comparable between RA+ and RO+ cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
We have proposed that molecules at the T cell / antigen presenting cell ( APC ) interface segregate according to size , with small `` accessory ' ' molecules ( e . g . , CD 2 , CD 4 , CD 8 , CD 28 , and CD 154 ) contributing to the formation of a close contact zone , within which the TCR engages peptide MHC , and from which large molecules are excluded ( Davis , S . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Simultaneous triggering of the costimulatory receptor CD 28 on T cells through its natural ligand CD 80 partly corrected the CD40L defect in patients with intermediate CD 4 T counts ( 200 500 ) , but not in AIDS patients . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Although normal alveolar macrophages ( AMs ) did not bear or bore low levels of costimulatory molecules , AMs from sarcoid patients with CD 4 T cell alveolitis upmodulated CD 80 , CD 86 , and CD 72 and expressed high levels of interleukin ( IL ) 15 ; lymphocytes accounting for T cell alveolitis expressed Th 1 type cytokines [ interferon ( IFN ) gamma and / or IL 2 ] and bore high levels of CD 5 and CD 28 but not of CD 152 molecules . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Addition of IL 2 , anti CD 28 antibodies , or phorbol esters , but not IL 1 , IL 4 , or ionomycin , blocked CD 4 mediated inhibition and restored the response to levels equal or higher than those of cultures activated by anti CD 3 alone . ^^^ Although CD 28 ligation does not produce any clear change in the tyrosine phosphorylation pattern in lysates from cells activated by indirectly bound anti CD 3 plus anti CD 4 antibodies , the analysis of active forms of the MAP kinase ERK suggests that downstream signaling pathways involved in IL 2 gene activation can be differentially activated depending on the direct or indirect CD 3 CD4 adsorption and CD 28 ligation . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Surprisingly , B . abortus induced down regulation of CD 28 and up regulation of B7 . 2 on murine CD 4 ( + ) and CD 8 ( + ) T cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
CD 28 co stimulation results in down regulation of lymphotactin expression in human CD 4 ( + ) but not CD 8 ( + ) T cells via an IL 2 dependent mechanism . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Flow cytometric analysis demonstrates that mPEG modification dramatically decreases antibody recognition of multiple molecules involved in essential cell : cell interactions , including both T cell molecules ( CD 2 , CD 3 , CD 4 , CD 8 , CD 28 , CD11a , CD62L ) and antigen presenting cell ( APC ) molecules ( CD 80 , CD 58 , CD62L ) likely preventing the initial adhesion and costimulatory events necessary for immune recognition and response . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Signals provided by coactivating molecules such as CD 4 and CD 28 also influence the fate of immature thymocytes . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Compromised OX 40 function in CD 28 deficient mice is linked with failure to develop CXC chemokine receptor 5 positive CD 4 cells and germinal centers . ^^^ These data suggest that CD 28 dependent OX 40 ligation of CD 4 T cells at the time of priming is linked with upregulation of CXCR 5 expression , and migration of T cells into B cell areas to support germinal center formation . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Analysis of 4 1BB ligand ( 4 1BBL ) deficient mice and of mice lacking both 4 1BBL and CD 28 reveals a role for 4 1BBL in skin allograft rejection and in the cytotoxic T cell response to influenza virus . 4 1BB ligand ( 4 1BBL ) is a member of the TNF family expressed on activated APC . 4 1BBL binds to 4 1BB ( CD 137 ) on activated CD 4 and CD 8 T cells and in conjunction with strong signals through the TCR provides a CD 28 independent costimulatory signal leading to high level IL 2 production by primary resting T cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The secondary signal for the activation of CD 4 ( + ) and CD 8 ( + ) T cells was provided with anti CD 18 mAb ( TS1 / 18 ) and anti CD 28 mAb ( 9 . 3 ) in the first and the second assay respectively . ^^^ RESULTS : CD 4 ( + ) and CD 8 ( + ) T cells were activated by cross linking CD 3 and CD 18 in the first assay ( CD3 / CD18 ) and CD 3 and CD 28 in the second assay ( CD3 / CD28 ) . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
On day 6 after alphaCD3 / alphaCD28 suture stimulation , T cell subpopulations expressing CD 3 , CD 4 , CD 8 , CD 28 , and CD45RO were enhanced . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Increased production of IFN gamma in UA could be attributed to the expansion of an unusual subset of T cells , CD 4 ( + ) CD 28 ( null ) T cells . ^^^ IFN gamma is produced by CD 4 ( + ) CD 28 ( null ) T cells , which are expanded in UA and distinctly low in SA and controls . ^^^ The emergence of CD 4 ( + ) CD 28 ( null ) T cells may result from persistent antigenic stimulation . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Anti CD 3 induced cytotoxicity of T cells from CD 4 knockout mice and CD 4 depleted nylon wool nonadherent spleen cells from wild type mice was inhibited by anti B 7 2 mAb , implying that B 7 2 is able to bind directly to CD 28 on CD8+ T cells and costimulate their activation . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In addition , this association was enhanced following independent ligation of the CD 2 , CD 4 , or CD 28 costimulatory molecules , but not of CD 5 or CD 6 surface receptors , correlating to the induced tyrosine phosphorylation of Emt . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In some patients the percentage of CD 28 ( ) T cells within the CD 4 ( + ) subset was increased up to 80 % . ^^^ These CD 4 ( + ) CD 28 ( ) T cells did not proliferate in a mixed lymphocyte culture ( MLC ) against allogeneic PBMC . ^^^ After enrichment for CD 4 ( + ) CD 28 ( + ) T cells , the proliferative response in the MLC was recovered , but this response was still lower than the proliferative response from control T cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The relative contribution of T cell receptor versus CD 28 mediated signals in co stimulation of resting CD 4 T cells is thought to influence their functional differentiation towards T helper ( Th ) 1 versus Th 2 subsets . ^^^ We have used a conventional and a mitogenic CD 28 specific monoclonal antibody to assess the effect of polyclonal T cell activation through CD 28 alone on CD 4 subset differentiation . ^^^ We suggest that CD 28 dependent induction of GATA 3 in concert with other transcription factors , which are preferentially induced by strong CD 28 signals , primes CD 4 T cells for IL 4 dependent Th 2 differentiaton . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
We performed in vitro kinase assays on JNK in CD 4 ( + ) T cells , from young and old mice , activated by antibodies to CD 3 , CD 4 , and CD 28 , and found a approximately 2 fold decline in JNK activity at the peak of activation , but no significant change in the kinetics of stimulation or in the steady state expression of JNK . ^^^ Aging does not , however , alter the level of Ras activation by anti CD3 / CD4 + / anti CD 28 or change the level of Ras protein in CD 4 ( + ) cells , suggesting that the JNK defect is due to changes in the regulation of other upstream regulators . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
We found that aging led to significant declines in the ability of mouse CD 4 ( + ) T cells to respond to CD 3 and CD 28 stimuli by induction of the cyclin dependent kinases CDK 2 , CDK 4 , and CDK 6 , whether the defect was assessed by protein level or functional activity . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Recent evidence indicates that RA patients carry expanded CD 4 clonotypes which are characterized by deficient CD 28 expression and autoreactivity . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The first results from the engagement of the TCR : CD 3 : CD 4 complex , and the second from the interaction of CD 28 with the B 7 family of co stimulatory molecules . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The pathogenesis of rheumatoid arthritis ( RA ) is a consequence of the activation of T cells by as yet unknown antigens and the co stimulatory molecules CD 4 and CD 28 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Using pigeon cytochrome c presenting fibroblast antigen presenting cells transfected with 4 1BB ligand ( 4 1BBL ) , we show that engaging 4 1BB on naive CD 4 cells promotes proliferation , cell cycle progression and IL 2 secretion , and suppresses cell death , all to a similar extent as B 7 1 engagement of CD 28 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Activation of T cells requires co stimulation of the TCR and accessory receptors like CD 2 , CD 4 , CD 8 , CD11a or CD 28 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Since the importance of MEK / ERK in the initial steps of viral replication is poorly understood , we have examined the role of MEK / ERK signaling in the CD 3 and CD 28 ( CD3 / CD28 ) mediated activation of HIV 1 replication in resting peripheral blood CD 4 ( + ) T lymphocytes infected with X 4 or R 5 HIV 1 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
UA 04 cells express CD3low , CD 4 , TCR 2low , MHC class 2 , and CD 28 antigens on their surface . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
This suggests that the p 38 MAPK pathway is required for induction of full AP 1 binding activity in human CD 4 ( + ) T cells co stimulated with A6H or CD 28 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
We determined B lymphocytes ( CD 40 ( + ) ) , T lymphocytes ( CD 3 ( + ) ) , T helper ( CD 4 ( + ) ) , and T suppressor ( CD 8 ( + ) ) cells and the expression of costimulatory signals B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) on B cells and their counter receptors CD 28 and CTLA 4 on T cells by fluorescence activated cell sorting analysis . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
CD 28 , Ox 40 , LFA 1 , and CD 4 modulation of Th1 / Th2 differentiation is directly dependent on the dose of antigen . ^^^ Reports suggest that molecules such as CD 4 , CD 28 , and Ox 40 support Th 2 differentiation and suppress Th 1 differentiation , whereas others such as LFA 1 support Th 1 responses and suppress Th 2 responses . ^^^ We have used this system to confirm that CD 4 , CD 28 , and Ox 40 interactions can promote , and LFA 1 interactions can suppress , differentiation of cells secreting the Th 2 cytokines IL 5 and IL 13 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
A simple culture system devoid of antigen presenting cells was used to examine the ability of immobilized antibodies to lymphocyte function associated antigen 1 ( LFA 1 ) ( CD11a ) , CD 28 and CD 4 or CD 8 to modulate the responses of normal murine CD4+ and CD8+ lymph node T cells to immobilized anti CD 3 antibody and interleukin 2 ( IL 2 ) . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
However , we found that stimulation of PBLs with maximal CD 28 costimulation , using beads coupled to Abs against CD 3 and CD 28 , led to a very prolonged expression of CD 154 on CD 4 cells ( > 4 days ) that was dependent upon autocrine IL 2 production . ^^^ Previously activated CD 4 T cells could respond to IL 2 , or the related cytokine IL 15 , by de novo CD 154 production and expression without requiring an additional signal from CD 3 and CD 28 . ^^^ These results provide evidence that CD 28 costimulation of CD 4 T cells , through autocrine IL 2 production , maintains high levels of CD 154 expression . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Only a modest increase in intracellular oxygen species was found in thymocytes stimulated by strong cross linking of TCR together with CD 4 or CD 28 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Cofilin is dephosphorylated on phosphoserine residues following co stimulation via accessory receptors such as CD 2 , CD 4 , CD 8 or CD 28 , but not in response to TCR engagement alone . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
All these clones were found to express a CD 3 ( + ) , TCRalphabeta ( + ) , CD 8 ( + ) , CD 4 ( ) , CD 28 ( ) phenotype . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Therefore , CD 28 ( + ) CD 4 ( + ) T cells are sufficient for clearance of avirulent Salmonella in naive hosts , whereas CD 4 ( + ) T cells and specific antibodies are required for protection from virulent Salmonella in immune hosts . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Primary stimulation of naive CD 4 ( + ) T cells with anti CD 28 mAb in the presence of IL 2 , but in the absence of anti CD 3 mAb induces preferential production of IL 4 and expression of CCR 4 mRNA after secondary stimulation with anti CD 3 , indicating the preferential differentiation of Th 2 cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In vivo , antigen presenting cells ( APCs ) activate CD 4 T cells in part by signaling through the T cell receptor and CD 28 , yet cells stimulated in this manner are susceptible to HIV 1 infection . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Patients with MG had lower percentages of CD 8 ( + ) CD 28 ( + ) cells , augmented percentages of CD 4 ( + ) CD 80 ( + ) , CD 4 ( + ) CD 86 ( + ) , CD 8 ( + ) CD 80 ( + ) , CD 8 ( + ) CD 86 ( + ) , CD 14 ( + ) CD 80 ( + ) , and CD 14 ( + ) CD 86 ( + ) cells , and similar levels of cells expressing CD 40 and CD40L and of B cells expressing CD 80 and CD 86 compared to the controls . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
These involved ( a ) increased plasma levels of neopterin and sTNF receptor indicating elevated IFNgamma and TNF cytokine activity ; ( b ) increased proportion of mature ( CD45RO ) versus nave ( CD45RA ) T cells ; and ( c ) a diverse group of related changes including impaired proliferative response , reduced T cells , CD 28 and CD 25 expression , B cell percentage and lower CD 4 : CD 8 ratios and increased HLA DR expression . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The subset of CD 4 ( + ) CD 28 ( null ) T cells was expanded in patients with UA and infrequent in patients with stable angina ( median frequencies : 10 . 8 % versus 1 . 5 % , P < 0 . 001 ) . ^^^ CD 4 ( + ) CD 28 ( null ) T cells included a large monoclonal population , with 59 clonotypes isolated from 20 UA patients . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Despite lacking CD 28 molecules , these unusual CD 4 T cells undergo clonal proliferation and form large and long lived clonal populations . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Together , our results demonstrate that ICOS regulates both CD 28 dependent and CD 28 independent CD 4 ( + ) subset ( Th 1 and Th 2 ) responses but not CTL responses in vivo . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In spite of lacking CD 28 expression , DP T cells showed similar proliferative ability and apoptosis sensitivity to CD 4 SP and CD 8 SP T cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
RESULTS : ( 1 ) In all cases of UC , colonic lamina propria contained numerous basal aggregates composed of lymphocytes , follicular dendritic cells , and CD80 / B7 . 1 positive dendritic cells . ( 2 ) CD 4 ( + ) CD 28 ( ) alphabeta T cells and B cells were the dominant cell types in the aggregates . ( 3 ) The aggregates contained a large fraction of cells that are normally associated with the epithelium : that is , gammadelta T cells ( 11 ( 7 ) % ) and alpha ( E ) beta ( 7 ) ( + ) cells ( 26 ( 13 ) % ) . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
CD 28 deficient CD 25 ( + ) CD 4 ( + ) T cells can also suppress normal T cells , indicating that CD 28 is dispensable for activation of the regulatory T cells . ^^^ Thus , the CD 25 ( + ) CD 4 ( + ) regulatory T cell population engaged in dominant self tolerance may require CTLA 4 but not CD 28 as a costimulatory molecule for its functional activation . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Response differences between human CD 4 ( + ) and CD 8 ( + ) T cells during CD 28 costimulation : implications for immune cell based therapies and studies related to the expansion of double positive T cells during aging . ^^^ The CD 8 ( + ) but not the CD 4 ( + ) subset developed a reversible double positive phenotype during CD 28 costimulation . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In univariate analyses , the counts of the following subsets showed a significant inverse correlation with infection rates : total B cells , IgD ( + ) B cells , IgD ( ) B cells , total CD 4 T cells , CD 28 ( + ) CD 4 T cells , CD 28 ( ) CD 4 T cells , CD45RA ( low / ) CD 4 T cells and monocytes . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
We recently reported that aging is accompanied by the emergence of CD 4 ( + ) CD 28 ( null ) T cells , a functionally aberrant lymphocyte subset rarely seen in individuals younger than 40 years . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Bacterial pathogens induce abscess formation by CD 4 ( + ) T cell activation via the CD 28 B7 2 costimulatory pathway . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 28 ( null ) T cells are oligoclonal lymphocytes rarely found in healthy individuals younger than 40 yr , but are found in high frequencies in elderly individuals and in patients with chronic inflammatory diseases . ^^^ In this study , we show that CD 4 ( + ) CD 28 ( null ) T cells are protected from undergoing activation induced cell death . ^^^ Consistent with previous data showing protection of CD 28 ( null ) cells from spontaneous cell death , the present studies unequivocally show dysregulation of apoptotic pathways in CD 4 ( + ) CD 28 ( null ) T cells that favor their clonal outgrowth and maintenance in vivo . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
To identify intrinsic defects in lupus , we studied short term , CD 4 ( + ) T cell lines that were established from 16 lupus patients ( active or inactive ) and 15 normal subjects by stimulating once with anti CD 3 , anti CD 28 , and IL 2 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Leucocyte number and viability were essentially unchanged from freshly obtained blood , but the expression of cell surface lymphocyte activation antigens ( CD 3 , CD 4 , CD 28 , CD 2 , CD 45 ) decreased rapidly within the first 24 h and continued to fall to less than 20 % of original levels by d 9 of storage at 4 degrees C . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In secondary lymphoid organs , CD 4 ( + ) CXCR 5 ( + ) cells lose expression of CCR 7 , which allows them to localize to B cell follicles and germinal centers where they express high levels of CD 40 ligand ( CD40L ) , a costimulatory molecule required for B cell activation and inducible costimulator ( ICOS ) , a recently identified costimulatory molecule of the CD 28 family . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Tg mice immunized with the FC / MUC1 fusion cells proliferate in response to MUC 1 antigen by a mechanism dependent on the function of CD 4 , major histocompatibility complex ( MHC ) class 2 , B 7 1 , B 7 2 , CD 28 , CD 40 and CD 40 ligand . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Here , we report that the appearance of Vbeta 5 ( ) CD 4 ( + ) T cells is CD 28 dependent . ^^^ The diversity of the revised TCRs , the minimal mouse to mouse variation in the expressed endogenous Vbeta repertoire , the atypical length of junctional sequences , and the CD 28 dependence of the accumulation of Vbeta 5 ( ) CD 4 ( + ) T cells all point to their extrathymic origin . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Compared with healthy controls , CD 4 and CD 8 T cells from patients with HIV and / or PTB expressed more activation markers ( HLA DR , CD 38 ) ; their CD 8 T cells expressed more CD 95 ( pre apoptosis ) and less CD 28 ( co stimulatory receptor ) . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The numbers of CD 4 cells that expressed CD 28 increased from a median of 308 10 10 ( 6 ) / l at baseline to 477 10 10 ( 6 ) / l at week 48 . ^^^ Higher baseline plasma HIV 1 RNA levels predicted the magnitude of early CD 4 ( r = 0 . 35 ; P = 0 . 01 ) , memory CD 4 ( r = 0 . 38 ; P = 0 . 001 ) and CD 28 CD4 cell ( r = 0 . 29 ; P = 0 . 01 ) restoration but was not related to second phase changes . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
This inhibition affected both CD 4 and CD 8 T cells and was only partially reversed by costimulation with interleukin 2 or CD 28 . gp 105 strongly inducted TNF alpha production by monocytes . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
OX 40 has been shown to participate in regulating clonal expansion and memory development of CD 4 T cells and may synergize with CD 28 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The plasma levels of sTNFR 2 , sCD 27 , b ( 2 ) m , IL 12 , and IgG were inversely correlated with numbers of CD 4 ( + ) T cells , the proportion of nave ( CD45RA ( + ) CD 27 ( + ) ) CD 8 ( + ) T cells , and the proportion of CD 8 ( + ) T cells expressing CD 28 ( CD 8 ( + ) CD 28 ( + ) ) were positively correlated with the proportions of activated ( HLA DR ( + ) CD 38 ( + ) ) CD 4 ( + ) T cells , as well as activated ( HLA DR ( + ) CD 38 ( + ) ) CD 8 ( + ) T cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Differential requirement of CD 28 for IL 12 receptor expression and function in CD 4 ( + ) and CD 8 ( + ) T cells . ^^^ CD 28 ( / ) CD 4 ( + ) but not CD 8 ( + ) cells exhibited a defect in IL 12Rbeta1 expression that was associated with a marked decrease in IL 12 binding as well as IL 12 induced IFN gamma production . ^^^ IL 2 could restore IL 12R expression to CD 28 ( / ) CD 4 ( + ) cells , however , this occurred independently of IL 2 induced proliferation . ^^^ Thus , these findings identify distinct requirements for CD 28 in the capacity of CD 4 ( + ) and CD 8 ( + ) cells to respond maximally to IL 12 . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Robust IL 2 secretion and T cell proliferation were observed when the same CD 4 CC49 zeta T cells were stimulated through the CD 28 receptor and endogenous T cell receptor . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
There were also more cells expressing CD 28 , SLe ( 10 ) , CD 4 , HLA DR , and NU B 2 in H . pylori positive ulcer patients than in non ulcer patients and healthy volunteers . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
By quantifying graft vs host disease alloresponses in vivo , we demonstrate that both CD 4 ( + ) and CD 8 ( + ) T cell mediated alloresponses are regulated by 4 1BB / 4 1BB ligand interactions to approximately the same extent . 4 1BB receptor facilitated CD 4 ( + ) T cell mediated alloresponses were partly CD 28 independent . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
CONCLUSION : Patients with RA have an expanded and unusual subset of CD 4 T cells that infiltrates the tissue lesions and is characterized by a deficiency of CD 28 , the expression of CD 8 alphaalpha homodimers , and the expression of several types of HLA class 1 recognizing NK receptors . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Abundant numbers of in vivo activated ( CD 69 ( high ) CD 44 ( high ) CD 28 ( high ) ) NK 1 ( + ) and NK 1 ( ) CD 4 ( + ) T cells were isolated from the inflamed colonic lamina propria ( cLP ) of transplanted mice with IBD that produced large amounts of TNF alpha and IFN gamma but low amounts of IL 4 and IL 10 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
These results show that Ag stimulated CD 4 T cells produce IL 2 in a polarized fashion and undergo CD 28 independent TCR redistribution in vivo . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In the absence of TCR ligation CD 28 stimulation induced Th 2 differentiation of memory but not of naive CD 4 ( + ) T cells , whereas costimulation via CD 28 and the TCR enhanced Th 2 differentiation from naive T cells but suppressed it from memory T cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
There was no difference in leukocyte distribution , CD 28 , CD 56 , CD 4 , CD8 / CD45 , colony forming units granulocyte / monocytes formation , peripheral blood mononuclear cell apoptotic rate , or serum TNF , TNF R 1 , TNF R 2 , IL 6 , soluble IL 6R , and IL 1 between the groups . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
CD 8 ( + ) CD 28 ( ) T cells suppress alloresponse of CD 4 ( + ) T cells both in primates and rodents . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Signaling via the CD 28 molecule plays a particular role in the activation of human CD 4 ( + ) CD45R0 ( + ) T cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The effects of Nef on CD 28 , CD 4 , CD 3 and class 1 MHC expression are all genetically separable , indicating that all are selected independently . ^^^ The likely consequence of the concerted down regulation of CD 28 , CD 4 and / or CD 3 by Nef is disruption of antigen specific signaling machineries in infected T cells following a productive antigen recognition event . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Isolated peripheral blood lymphocytes were stained for the lymphocyte subset markers CD 4 , CD 8 , CD 19 , their respective activation markers CD 25 , HLA DR , CD 38 , and the costimulatory molecules CD40L , CD 28 , CD 40 , CD 80 , and CD 86 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
CD 28 is a costimulatory receptor expressed in most CD 4 ( + ) T cells . ^^^ Four subsets of CD 28 ( ) , CD 28 ( low ) , CD 28 ( int ) , and CD 28 ( high ) peripheral blood human CD 4 ( + ) T cells were defined using three color flow cytometry . ^^^ RA patients , whether active or inactive , showed a distinct phenotype when compared to healthy donors : ( 1 ) the percentage of CD 4 ( + ) CD 28 ( high ) cells was increased twofold and the CD 4 ( + ) CD 28 ( low ) subset was reduced twofold ( P < 0 . 01 ) and ( 2 ) the CD 4 ( + ) CD 28 ( high ) cells from RA patients showed an in vivo activated phenotype , CD45RO ( + ) CD 5 ( high ) IL 2Ralpha ( + ) ( P < 0 . 01 ) . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Increased naive CD8+ and CD 28 +CD8+ T cells showed a positive correlation with % CD 4 and a negative correlation with % CD 8 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The genetic determinants that confer risk for rheumatoid vasculitis are not known , but patients with vascular complications are known to have an expansion of CD 4 ( + ) CD 28 ( null ) T cells , a cell population potentially involved in endothelial damage . ^^^ CD 4 ( + ) CD 28 ( null ) T cell clones isolated from RA patients with vasculitis were found to express killer cell immunoglobulin like receptors ( KIRs ) with the stimulatory KIR2DS2 often present in the absence of opposing inhibitory receptors with related specificities . ^^^ Specifically , KIR2DS2 in conjunction with the appropriate HLA C ligand may have a role in vascular damage by regulating CD 4 ( + ) CD 28 ( null ) T cells . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The frequency of cells expressing activation markers CD 26 , CD 28 , CD 69 , and HLA DR was enhanced in AV2S3 positive versus AV2S3 negative BAL CD 4 ( + ) T cell subsets . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Increasing the strength of signal by providing either costimulation with CD 28 cross linking or the addition of IL 2 to a maximal anti CD 3 stimulus resulted in a modest induction of proliferation and the loss of observable suppression in cocultures of CD 4 ( + ) CD 25 ( high ) regulatory cells and CD 4 ( + ) CD 25 ( ) responder cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
To compare CD 4 vs CD 8 responses to 4 1BBL under similar conditions of antigenic stimulation , we performed MLRs with purified CD 4 or CD 8 responders from CD 28 ( + / + ) and CD 28 ( / ) mice . ^^^ CD 4 and CD 8 T cells exhibited enhanced proliferation and earlier cell division when stimulated with anti CD 3 plus anti CD 28 compared with anti CD 3 plus 4 1BBL , and both subsets responded comparably to anti CD 3 plus 4 1BBL . ^^^ These data support the idea that CD 28 plays a primary role in initial T cell expansion , whereas 4 1BB / 4 1BBL sustains both CD 4 and CD 8 T cell responses , as well as enhances cell division and T cell effector function . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
CD45RB ( high ) CD 4 ( + ) cells from CD 28 ( / ) mice failed to induce mucosal inflammation in SCID recipients . ^^^ Of note , CD 25 ( + ) CD 4 ( + ) cells from CD 28 ( / ) mice similar to those from the wild type mice were efficient to prevent intestinal mucosal inflammation induced by the wild type CD45RB ( high ) cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
A number of other coreceptor molecules , including CD 4 , CD 8 , and CD 28 , have now been characterized that also play important roles in initiating or amplifying the activation of the T cell . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Lowered expression of CD 4 is correlated with a reduced expression of CD 3 , as well as with a decreased amount of CD 28 and CD95Fas . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
There was a positive correlation of percentage of CD 28 ( + ) CD 57 ( ) CD 8 ( + ) T cells with CD 4 ( + ) T cell percentages in each HIV infected group . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The immunological mechanisms involved in this antitumor effect were suggested by a decisively increased secretion of tumor necrosis factor alpha TNFTnTNa and IFN gamma from CD 4 ( + ) and CD 8 ( + ) T cells and a markedly up regulated expression on CD 8 ( + ) T cells of high affinity IL 2 receptor alpha chain ( CD 25 ) , costimulatory molecule CD 28 , and adhesion molecule lymphocyte function associated antigen 2 ( LFA 2 / CD2 ) . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Splenic CD 4 T cells ( approximately 90 % purity ) or CD 8 T cells ( approximately 85 % purity ) were incubated with agonists which act at the plasma membrane receptor level [ anti ( alpha ) CD3 / anti ( alpha ) CD 28 ] , the intracellular level ( PMA / Ionomycin ) , or at both the receptor and intracellular levels ( alphaCD3 / PMA ) . ^^^ Conversely , IL 2 production by alphaCD3 / alpha CD 28 stimulated CD 4 T cells was reduced in FO fed animals ( P < 0 . 0001 ) . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Aging and chronic inflammatory syndromes , such as rheumatoid arthritis , are associated with high frequencies of CD 4 ( + ) CD 28 ( null ) T cells , which are rarely seen in healthy individuals younger than 40 years . ^^^ This effect of TNF alpha was reversible ; however , continuous culture of CD 4 ( + ) CD 28 ( + ) T cell clones in TNF alpha resulted in the appearance of a CD 28 ( null ) subset . ^^^ We propose that the emergence of CD 4 ( + ) CD 28 ( null ) T cells in vivo is facilitated by increased production of TNF alpha . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Concentrations of CD 8 ( + ) and CD 4 ( + ) T cells lacking CD 28 and CD 95 on their surface increased more than those of cells expressing these receptors . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Naive CD 4 T cells inhibit CD 28 costimulated R 5 HIV replication in memory CD 4 T cells . ^^^ Our results show that inhibitory mechanisms , expressed predominantly by strongly stimulated naive CD 4 T cells and mediated independently of CCR 5 binding chemokines , play a role in the inhibition of R 5 HIV replication in CD 4 T cells upon CD 28 costimulation . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
We show that OX 40 ( CD 134 ) is required with CD 28 for the survival of CD 4 T cells following antigen driven expansion . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Substitution of Y223F disrupts the ability of simian immunodeficiency virus ( SIV ) Nef to down modulate major histocompatibility complex ( MHC ) class 1 from the cell surface but has no effect on other Nef functions , such as down regulation of CD 4 , CD 28 , and CD 3 cell surface expression or stimulation of viral replication and enhancement of virion infectivity . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Signal transduction regions studied include the TCR zeta chain , the CD 4 coreceptor , the lck protein tyrosine kinase , and the CD 28 costimulatory receptor . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
BACKGROUND : Blockade of the CD 40 and CD 28 pathways is a powerful strategy to inhibit CD 4 mediated alloimmune responses . ^^^ In this study , we examine the relative roles of the CD 40 and CD 28 pathways on CD 4 mediated allograft rejection responses , and further characterize the role of these pathways on CD4+ T cell activation , priming for cytokine production , and cell proliferation in response to alloantigen in vivo . ^^^ METHODS : BALB / c skin allografts were transplanted onto C57BL / 6 Rag 1 / recipients reconstituted with CD 4 cells from CD 28 / or CD40L / donors . ^^^ To investigate the role of CD 40 and CD 28 blockade on CD 4 cell proliferation , the fluorescein dye carboxyfluorescein diacetate succinimidyl ester was used . ^^^ CD40L blockade was equivalent to or inferior to CD 28 blockade in inhibition of in vivo CD 4 cell activation , priming for cytokine production , and proliferation responses to alloantigen . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Major histocompatibility complex ( MHC ) class 2 expressing cells as well as CD 3 , CD 4 , CD 25 , CD 28 , CD 68 , CD 80 , and CD 83 positive cells were localized immunohistologically . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In vitro TCR stimulation with or without CD 28 costimulation of CD 4 ( + ) T cells did not lead to the enhancement of gene transcription of these PTKs . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In addition , adoptive transfers of CD 4 ( + ) T cells lacking either the costimulatory molecule CD 28 or the ability to produce IFN gamma were inadequate to restore injury phenotype . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Coimmobilization of mAb to CD 4 , CD11a , and / or CD 28 increased the size of these clones but each exerted different effects on their cytokine profiles . ^^^ However , whereas 0 6 % of clones obtained with mAb to CD11a or CD 28 produced IL 4 , 10 40 % of those coactivated with anti CD 4 mAb were IL 4 producers . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Using DO11 . 10 TCR transgenic T cells , we confirmed that CD 28 expression was dispensable for homeostatic expansion , and showed that the presence of endogenous CD 4 ( + ) CD 25 ( + ) regulatory cells did not detectably influence homeostatic expansion . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
VPAC ( 2 ) R null ( VPAC ( 2 ) R ( / ) ) mice on a C57BL / 6 background are shown here to have normal basic immune characteristics , including serum Ig concentrations , blood levels of all leukocytes , and spleen number of total T cells ( CD 3 ( + ) ) and T cells bearing CD 4 , CD 8 , and CD 28 . ^^^ Cytokine production by splenic CD 4 ( + ) T cells , stimulated with adherent anti CD 3 plus anti CD 28 antibodies , revealed higher levels of IL 2 ( mean = 3 fold ) and IFN gamma ( mean = 3 fold ) , and lower levels of IL 4 ( mean = one fifth ) in VPAC ( 2 ) R null mice than wild type controls . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The composition of the T cell compartment was analyzed by flow cytometry and the sizes of three T cell subsets , CD 4 ( + ) CD45RO ( + ) cells , CD 4 ( + ) CD 28 ( null ) cells , and CD 8 ( + ) CD 28 ( null ) cells , were determined . ^^^ The sizes of the CD 4 ( + ) CD45RO ( + ) memory T cell and CD 4 ( + ) CD 28 ( null ) T cell subsets had no effect on the ability to mount anti influenza virus antibody responses . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Without CD 4 help , CD 8 rejection of pig xenografts requires CD 28 costimulation but not perforin killing . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In contrast , the simultaneous triggering of CD 3 and CD 28 was required for equivalent IL 2 production in control single positive CD 4 ( + ) thymocytes . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Characterization of CD 28 ( ) CD 4 ( + ) T cells in living kidney transplant patients with long term allograft acceptance . ^^^ CD 28 ( ) CD 4 ( + ) T cell subpopulation is expanded in kidney allograft patients with long graft survival . ^^^ To seek for the roles of CD 28 ( ) CD 4 ( + ) T cells in the long term acceptance of kidney allografts , we characterized this population by analyzing cell surface molecules , TCR 5 ( beta ) repertoire , mixed lymphocyte reaction ( MLR ) , and cytokine production . ^^^ The number of CD 28 ( ) CD 4 ( + ) T cells increased correlatively with time after transplantation in this group of patients . ^^^ The CD 28 ( ) CD 4 ( + ) T cells did not express detectable levels of CD 25 , CD 69 , 5 ( alpha ) 24 , or CTLA 4 but expressed heterogeneous amounts of CD 45 RA on the surface . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
CD 4 and CD 8 T cells expressed similar CD 25 profiles although CD 28 expression was unique in each subset . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In the second system , functional differentiation was induced in purified naive CD 4 T cells with cytokines and Abs to CD 3 and CD 28 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Poor responders were found to have significantly reduced CD 28 expression on both CD 4 ( + ) and CD 8 ( + ) cells , enhanced IL 10 generation from peripheral blood mononuclear cells ( PBMCs ) , higher plasma IL 12 levels and enhanced TNF alpha release from PBMCs . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
METHODS AND RESULTS : Gene profiling identified perforin , CD 161 , and members of the killer cell immunoglobulin like receptors as being differentially expressed in CD 4 ( + ) CD 28 ( null ) T cells , a T cell subset that preferentially infiltrates unstable plaque . ^^^ CD 161 appeared on CD 4 ( + ) CD 28 ( null ) T cells after stimulation , suggesting spontaneous activation of circulating CD 4 T cells in UA . ^^^ HUVEC cytolysis was dependent on granule exocytosis , as demonstrated by the paralyzing effect of pretreating CD 4 ( + ) CD 28 ( null ) T cells with strontium . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Regulation of Lck activity by CD 4 and CD 28 in the immunological synapse . ^^^ Instead , the T cell accessory molecules CD 4 and CD 28 cooperated to induce autophosphorylation of Lck . ^^^ CD 4 recruited Lck to the T cell APC interface , whereas CD 28 sustained Lck activation . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
CD 28 median fluorescence intensity in CTLA 4 cells was twice that in CTLA 4+ cells ( 140 + / 5 . 3 versus 70 + / 2 . 28 , P < 0 . 00001 ) , whereas cells low in CD 28 and CD 4 , expressed more CTLA 4 ( P < 0 . 0001 ) . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
To investigate the changes of CD 28 and HLA DR molecules on CD4+ and CD8+ T cells during HIV infection , we classified 130 HIV infected Koreans into four groups by the CD 4 level as follows : group 1 ( > or = 500 cells / mm3 ) , group 2 ( 201 499 cells / mm3 ) , group 3 ( 51 200 cells / mm3 ) , and group 4 ( < or = 50 cells / mm3 ) . ^^^ In CD4+ T cells , the proportion of CD 28 expression decreased significantly with the CD 4 level while the proportion of HLA DR expression increased gradually . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Co ligation of TCR and CD 4 co receptor on T cells by MHC 2 peptide complexes ( signal 1 ) leads to various T cell functions depending on the nature of TCR and CD 4 co ligation , and whether costimulatory receptors ( signal 2 ) such as CD 28 , CTLA 4 , CD40L are involved in this interaction . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
There were no phenotypic differences in lymph node T cell subpopulations ( CD 3 , CD 4 , CD 8 , CD 28 , CD45RO ) between groups . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
PTEN expression was up regulated on RNA and protein level in freshly isolated human CD 4 ( + ) T cells following stimulation with CD 28 or CD 2 . ^^^ Intracellular staining revealed PTEN expression in CD 4 ( + ) T cell populations stimulated with anti CD 28 or anti CD 28 / anti CD 3 . ^^^ Inhibition of PTEN expression by antisense oligonucleotides in CD 4 ( + ) T cells stimulated with non mitogenic anti CD 28 resulted in massively increased proliferation , which was sensitive to the phosphatidylinositol 3 kinase ( PI 3 K ) inhibitor wortmannin . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Killer Ig like receptors ( KIRs ) are expressed on CD 4 ( + ) CD 28 ( null ) T cells , a highly oligoclonal subset of T cells that is expanded in patients with rheumatoid arthritis . ^^^ To determine whether KIR expression is a consequence of clonal expansion and replicative senescence , multiple CD 4 ( + ) CD 28 ( null ) T cell clones expressing the in vivo dominant TCR beta chain sequences were identified in three patients and analyzed for their KIR gene expression pattern . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
T cell costimulation via CD 28 and CD40L , however , is necessary and sufficient for activation and expansion of CD 4 ( + ) T cells in vivo . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Surface expression of CD 25 , CD 28 , CD 40 , CD 54 , CD 80 , CD 86 and CTLA 4 was evaluated on CD 3 , CD 4 , CD 8 , CD 14 and CD 19 PBMC subpopulations by three colour flow cytometry . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Further , the engagement of CD 160 receptors on normal clonal T lymphocyte populations lacking CD 4 , CD 8 and CD 28 molecules by MHC class 1 molecules results in an increased CD 3 induced cell proliferation . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Using adoptive transfer of TCR transgenic CD 8 T cells , this effect of IL 12 is shown to be independent of CD 4 T cells and to require costimulation provided by CD 28 and possibly LFA 1 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
We examined the co stimulatory activity of H4 / ICOS on murine activated CD 4 ( + ) T cells and found that the cross linking of H4 / ICOS enhanced their proliferation , in addition to raising IFN gamma , IL 4 and IL 10 production to levels comparable to those induced by CD 28 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Our evidence that the activation of Lck activates in T cells apoptotic pathways which are counteracted by Vav , a signaling molecule that cooperates with CD 28 to boost TCR signals , suggests a novel role for costimulation in protecting T cells from CD 4 mediated cell death . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In this work , we identify for the first time the neoplastically transformed cells in MD as the CD 4 ( + ) major histocompatibility complex ( MHC ) class 1 ( hi ) , MHC class 2 ( hi ) , interleukin 2 receptor alpha chain positive , CD 28 ( lo / ) , phosphoprotein 38 negative ( pp 38 ( ) ) , glycoprotein B negative ( gB ( ) ) , alphabeta T cell receptor positive ( TCR ( + ) ) cells which uniquely overexpress a novel host encoded extracellular antigen that is also expressed by MDV transformed cell lines and recognized by the monoclonal antibody ( MAb ) AV 37 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
For this reason antiviral CD 4 ( + ) and CD 8 ( + ) T cell responses in CD 28 deficient mice were studied using two different viruses [ vesicular stomatitis virus and lymphocytic choriomeningitis virus ( LCMV ) ] . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Using blocking mAbs to costimulatory ligands and CD 4 T cells deficient in expression of their receptors , we found that the CD 4 T cell response requires CD 28 : B7 . 1 / B7 . 2 interactions , but not CTLA 4 or CD 40 CD40 ligand interactions . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In support of this hypothesis , infection of wild type or CD 28 ( / ) mice with T . gondii resulted in the increased expression of ICOS by activated CD 4 ( + ) and CD 8 ( + ) T cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
These analyses revealed 1 ) optimal peptides capable of eliciting specific responses by themselves at doses as low as 2 pg / ml , with each log increase in dose eliciting ever increasing frequencies of responding cells over a 4 to 5 log range ; 2 ) significant augmentation of response frequencies at all submaximal peptide doses by CD 28 and CD49d mediated costimulation ; 3 ) differential dose response and costimulatory characteristics for IFN gamma and IL 2 responses ; and 4 ) no association of activation requirements with the CD 27 defined CD 4 ( + ) T cell memory differentiation pathway . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
MUC1 tumor cell line was primarily mediated by CD 4 ( + ) T cells , and required Fas ligand , lymphotoxin alpha , CD 40 , CD 40 ligand and CD 28 , but not perforin , gammadelta T cells , IL 4 , IL 10 , IL 12 or tumor necrosis factor receptor 1 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
However , the expression of known cell surface accessory and adhesion molecules , such as CD 4 , CD 28 , leukocyte function associated antigen 1 , intercellular adhesion molecule 1 , and CTLA 4 , was not affected by beta SQDG ( 18 : 0 ) treatment . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Here we demonstrate that CD 4 ( + ) T cells from CD 28 deficient mice show increased susceptibility to Fas mediated apoptosis via a phosphatidylinositol 3 kinase ( PI3K ) dependent pathway . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In this study , we show that CD 4 T cells do not become AINR , but instead are rendered susceptible to Fas mediated activation induced cell death following stimulation through TCR and CD 28 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The ratio of CD 80 : CD 86 expression was significantly lower on CD 4 ( + ) and F4 / 80 ( + ) spleen cells in CD 28 peptide treated mice . ^^^ Peripheral deletion of Ag specific CD 4 ( + ) T cells occurs following in vivo blockade of CD 28 with synthetic CD 28 peptides . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Costimulation through the B 7 CD28 interaction is an important second signal for T cell activation , and previous studies have shown that CD 28 ( / ) mice infected with Toxoplasma gondii generate suboptimal CD 4 ( + ) T cell responses , associated with a defect in production of the T cell growth factor interleukin 2 ( IL 2 ) . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Ligation of CD 28 alone induced a set of short lived early response transcripts in both Jurkat T cells and primary CD 4 T cells , thus providing evidence that CD 28 engagement can affect gene regulation independently of TCR engagement . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
On coculture of `` naive ' ' CD 4 ( + ) T cells with mature DC in the presence of superantigen , ICOS was highly up regulated on T cells , but played only a secondary role in the CD 28 dominated release of TNF alpha and IFN gamma , and did not participate in the induction of IL 2 . ^^^ Cocultures of `` effector ' ' CD 4 ( + ) T cells with mature DC revealed CD 28 as the driving force for the secretion of IL 2 , IFN gamma , IL 6 , and IL 13 , with no apparent contribution of ICOS . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Compared with pre G CD 4 ( + ) T cells , alloantigen activated post G CD 4 ( + ) T cells preferentially expressed markers associated with memory T cells , in conjunction with reduced levels of CD 28 and CD62L . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The costimulation induced full activation of CD 4 ( + ) T cells with high levels of IL 2 production and cellular expansion that were comparable to those obtained on costimulation by CD 28 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The peripheral blood T cell subsets including CD 3 ( + ) , CD 4 ( + ) , CD 8 ( + ) , NK , CD 4 ( + ) / CD8 ( + ) ratio , CD 4 ( + ) CD 45 ( + ) , CD 4 ( + ) CD 29 ( + ) , CD 8 ( + ) CD 28 ( + ) and CD 8 ( + ) CD 28 were measured by flow cytometry in both groups before and one week after treatment . ^^^ After TACE , no significant changes occurred in CD 3 ( + ) , CD 4 ( + ) , CD 8 ( + ) , NK , CD 4 ( + ) / CD8 ( + ) , CD 4 ( + ) CD 29 ( + ) or CD 8 ( + ) CD 28 cells in group A , while significant decrease in CD 4 ( + ) CD 45 ( + ) and increase in CD 8 ( + ) CD 28 ( + ) cells were observed ( P < 0 . 05 and P < 0 . 001 , respectively ) . ^^^ In group B , CD 4 ( + ) and CD 4 ( + ) CD 29 ( + ) levels , together with CD 4 ( + ) / CD8 ( + ) ratio , were significantly lower than those before treatment ( P < 0 . 05 ) , but CD 8 ( + ) and CD 8 ( + ) CD 28 subsets were significantly higher ( P < 0 . 05 ) . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The proportion of CD 4 lymphocytes expressing CD 28 increased during the first and second years , but even after 3 years , CD 28 expression on CD 4 cells remained abnormally low . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Tumours were resected post biochemotherapy and analysed for the expression of CD 28 in CD 4 ( + ) and CD 8 ( + ) lymphocytes in areas where histopathological regressive changes had occurred , and close to tumour cells in areas of unaffected tumour growth using a double staining technique . ^^^ A high percentage of the lymphocytes in areas with regressive changes were found to be CD 4 ( + ) CD 28 ( ) . ^^^ In contrast , the vast majority of CD 4 ( + ) lymphocytes migrating close to the tumour cells were found to be CD 28 ( + ) ( P < 0 . 001 ) . ^^^ A difference in down regulation of the expression of CD 28 was found between CD 4 ( + ) and CD 8 ( + ) lymphocytes both in the areas of regressive changes and in the unaffected tumour areas . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Monoclonal Ab against CD 4 and CD 28 inhibited the proliferative response of ATL 1 for syngeneic B cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Conversely , augmenting CD 28 signals with an agonistic Ab bypassed the requirement for CD 4 ( + ) T help or CD 40 activation . ^^^ These results support a model in which activation induced up regulation of B 7 molecules on APC leads to increased CD 28 signaling and a commitment to cross priming of CD 4 dependent CTL . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
CD 28 , TNF receptor , and IL 12 are critical for CD 4 independent cross priming of therapeutic antitumor CD8+ T cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In correlation with their selective effects on humoral and cellular immune responses , these chemokines also differentially attract CD 4 ( + ) versus CD 8 ( + ) T cells and modulate CD 40 , CD 80 , and CD 86 expressed by B 220 ( + ) cells as well as CD 28 , 4 1BB , and gp 39 expression by CD 4 ( + ) and CD 8 ( + ) T cells in a dose dependent fashion . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Nef , an essential pathogenic determinant for human immunodeficiency virus type 1 , has multiple functions that include disruption of major histocompatibility complex class 1 molecules ( MHC 1 ) and CD 4 and CD 28 cell surface expression . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
We found that the tNef proteins were unable to down regulate the cell surface expression of major histocompatibility complex class 1 proteins , CD 4 , and CD 28 and neither stimulated SIV replication nor enhanced virion infectivity . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In a search for novel early T cell activation transcripts , we identified expressed sequence tags ( ESTs ) more abundantly expressed in normal human CD 4 ( + ) T lymphocytes fully activated by a 5 h exposure to CD 3 plus CD 28 mAbs , compared to the same cells stimulated with either CD 3 mAb or CD 28 mAb alone . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
These findings demonstrate there is a spectrum of associations between SIV , HIV 2 , and HIV 1 Nefs , and the TCR / CD3 complex , and suggest that down modulation of the TCR / CD3 complex occurs via association with subsets of cellular proteins that are different from those involved in CD 4 and CD 28 down modulation . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Accumulation of specific CD 28 ( ) IL 2 ( + ) helpers and loss of IFN gamma ( + ) IL 2 ( + ) CD 4 T cells may compromise specific CD 8 responses and , in turn , immunity to HIV . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
RESULTS : The patients studied showed a significant decrease of total lymphocyte count , T CD 4 ( + ) CD 3 ( + ) , CD 28 ( + ) , CD 8 ( + ) CD 28 ( + ) lymphocytes and natural killer ( NK ) CD 56 ( + ) CD 16 ( + ) CD 3 ( ) cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Mice genetically deficient in CD 28 , CD 40 , and CD40L were able to reject SVR tumors , but depletion of these mice of CD 8 , but not CD 4 cells led to rapid tumor growth . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
G ( 0 ) > G ( 1 ) phase progression by CD 28 deficient CD 4 ( + ) T cells or wild type T cells cultured in the presence of neutralizing anti B 7 mAbs was slowed , confirming that a synergy does exist between TCR and CD 28 signaling in the initial activation of the T cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Divergent telomerase and CD 28 expression patterns in human CD 4 and CD 8 T cells following repeated encounters with the same antigenic stimulus . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
It was previously demonstrated that both an in vivo protein ( pneumococcal surface protein A [ PspA ] ) and polysaccharide ( phosphorylcholine [ PC ] determinant of teichoic acid ) specific immunoglobulin ( Ig ) isotype response to Streptococcus pneumoniae was dependent on CD 4 ( + ) TCRalphabeta ( + ) T cells and B 7 dependent costimulation through CD 28 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
CD 4 T cell activation is positively ( CD 28 ) and negatively ( CTLA 4 ) regulated by the costimulatory ligands CD 80 and CD 86 . ^^^ We have previously shown that CD 28 signaling is absolutely required to prime naive CD 4 T cells to differentiate into effectors that provide help for germinal centers and class switched Ab responses . ^^^ In this study , we show that the requirement for CD 28 signaling is transient and effector CD 4 T cells do not require CD 28 signals to sustain their function . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
A cell based artificial antigen presenting cell coated with anti CD 3 and CD 28 antibodies enables rapid expansion and long term growth of CD 4 T lymphocytes . ^^^ K 32 cells loaded with anti CD 3 and anti CD 28 Ab ( K32 / CD3 / 28 ) induced more rapid CD 4 T cell expansion than CD3 / 28 coated beads . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The CD 86 ( + ) major histocompatibility complex class 2 ( + ) ( MHC 2 ) ( + ) cells and CD 28 ( + ) CD 4 ( + ) T cells were decreased by SST treatment , while CD 80 ( + ) MHC 2 ( + ) cells , CD 40 ( + ) MHC 2 ( + ) cells and CD 154 ( + ) CD 4 ( + ) T cells showed no change . ^^^ These data suggested that SST may suppress IL 4 production in CD 4 ( + ) T cells via influencing CD 28 CD86 interaction . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
CD 28 is the quintessential costimulatory molecule expressed on CD 4 ( + ) and CD 8 ( + ) T cells . ^^^ CD 4 ( + ) CD 28 ( null ) T cells constitutively expressed interleukin 12 ( IL 12 ) alpha and beta receptors , which were functional and allowed for the up regulation of the signal transducer and activator of transcription 4 ( STAT 4 ) dependent gene CD 161 . ^^^ Costimulation of the T cell and IL 12 receptors induced the transcription of CD 28 in approximately 50 % of CD 4 ( + ) CD 28 ( null ) T cell clones and lines . ^^^ The re expressed CD 28 was functional and restored the ability of CD 4 ( + ) CD 28 ( null ) T cells to express CD 25 and CD 40 ligand . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Flow cytometry was used to detect the count of CD 4 ( + ) T lymphocytes , CD 8 ( + ) T lymphocytes , and its different subsets including the naive cell subset ( CD 4 ( + ) CD45RA ( + ) CD62L ( + ) ) , activated cell subsets ( CD 4 ( + ) HLA DR ( + ) , CD 8 ( + ) HLA DR ( + ) , CD 8 ( + ) CD 38 ( + ) ) , functional cell subsets ( CD 4 ( + ) CD 28 ( + ) ) , and apoptotic cell subsets ( CD 4 ( + ) CD 95 ( + ) , CD 8 ( + ) CD 95 ( + ) ) . ^^^ The percentage of CD 4 ( + ) CD 28 ( + ) was 93 . 1 % + / 8 . 1 % in the control group , significantly higher than those in HIV infected person group ( 62 . 6 % + / 28 . 2 % ) and in AIDS patient group ( 56 . 9 % + / 26 . 4 % ) with a significant difference between any two groups ( all P < 0 . 01 ) . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Whereas ligation of CD 28 is known to provide a critical costimulatory signal for activation of CD 4 T cells , the requirement for CD 28 as a costimulatory signal during activation of CD 8 cells is less well defined . ^^^ These data demonstrate for the first time that CD 28 is critical for tolerance induction of CD 8 T cells , contrasting markedly with CD 28 independence of in vitro activation , and suggest that the role of CD28 / B7 interactions in peripheral tolerance of CD 8 T cells may differ significantly from that of CD 4 T cells . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Comparison with normal population , decreasing of CD 3 , CD 4 , NK cell and CD 28 in the 36 patients pre operation was statistically significant ( P < 0 . 01 ) . 2 . ^^^ Decreasing of CD 3 , CD 4 , NK cell and CD 28 was statistically significant post operation ( P < 0 . 05 ) . 3 . ^^^ In the non transfusion group change of CD 3 , CD 4 , CD 8 , NK cell and CD 28 post operation was statistically insignificant ( P > 0 . 05 ) . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Compared with controls , IL 2 induced a greater increase of naive and memory CD 4 T cells , lymphocyte expression of CD 28 and CD 25 ( P < 0 . 0001 ) and natural killer cells ( P < 0 . 001 ) . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Surprisingly , however , TGF beta 1 strongly inhibited production of IL 2 from nave CD 4 ( + ) T cells , even when CD 28 was engaged . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Then , CD 4 and CD 8 cell labelling was combined with CD 30 , CD 27 , CD 28 , CD45RA or CD45R0 staining , detection of intracellular interferon gamma ( IFN gamma ) or interleukin 4 ( IL 4 ) and analysis by three colour flow cytometry . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Instead , CD 4 ( + ) CD 28 ( null ) T cells express killer immunoglobulin like receptors ( KIRs ) with a preferential expression of the stimulatory receptor , CD158j . ^^^ The frequency of CD 4 ( + ) CD 28 ( null ) T cells in rheumatoid arthritis ( RA ) correlates with the risk for more severe disease . ^^^ However , the majority of CD 4 ( + ) CD 28 ( null ) T cell clones lacked the expression of KARAP / DAP12 . ^^^ CD 4 ( + ) CD 28 ( null ) T cells expressed DAP 10 and inhibition of phosphatidylinositol 3 kinase , which acts downstream of DAP 10 , inhibited JNK activation ; however , no interaction of DAP 10 with CD158j could be detected . ^^^ Costimulation by CD158j may contribute to the autoreactivity of CD 4 ( + ) CD 28 ( null ) T cells in RA . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Clonally expanded , autoreactive CD 4 ( + ) CD 28 ( null ) cells can be found in the peripheral blood of patients with rheumatoid arthritis and have been shown to be associated with severeextra articular disease manifestations . ^^^ We investigated the size of the CD 4 ( + ) CD 28 ( null ) compartment and the TCR beta chain repertoire of expanded CD 4 ( + ) clonotypes in 94 rheumatoid arthritis patients by complementarity determining region 3 ( CDR 3 ) length analysis ( spectratyping ) in the BV 6 and BV 14 TCR families , with primers specific for three arbitrarily chosen beta chain joining elements ( BJ1S2 , BJ2S3 and BJ2S7 ) . ^^^ The spectratyping results showed a strong correlation of the size of the CD 4 ( + ) CD 28 ( null ) compartment with the detected number of BV 14 clonotypes , whereas no association with BV 6 oligoclonality was found . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
H4 / ICOS mediated activation of JNK , but not ERK or p 38 , is partially dependent on the expression of CD 4 by the cells , whereas H4 / ICOS costimulation is partially independent on CD 28 expression . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Considerable progress has been made in characterizing four key sets of interactions controlling antigen responsiveness in T cells , involving the following : the T cell antigen receptor , its coreceptors CD 4 and CD 8 , the costimulatory receptors CD 28 and CTLA 4 , and the accessory molecule CD 2 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
These have included interactions involving the T cell antigen receptor ( TCR ) itself , its coreceptors CD 4 and CD 8 , the accessory molecule CD 2 , and the costimulatory receptors CD 28 and CTLA 4 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
We define here a G ( 0 ) > G ( 1 ) commitment point that occurs 3 to 5 h after CD 3 and CD 28 stimulation of human CD 4 or CD 8 T cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
This could be a result of a common mechanism involving perturbation in the formation of the central supramolecular activation cluster of the immunological synapse by impaired recruitment of CD 4 and CD 28 , thereby resulting in severely reduced lck activation . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Evidence for activation of CD 4 ( + ) / TCRalphabeta ( + ) and TCRgammadelta ( + ) T cells relied on changes similar to those described for CD 8 ( + ) / TCRalphabeta ( + ) although less pronounced , except for higher levels of both CD 5 and CD 28 in the absence of reactivity for CD11c on CD 4 ( + ) / TCRalphabeta ( + ) T cells and higher levels of CD 161 and CD 94 on TCRgammadelta ( + ) T cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Interestingly , the percentage of PB CD 4 cells expressing CD 28 was lower in JIA than controls . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Likewise , purified CD 4 ( + ) T cells from IDDM patients produced elevated levels of the cytokine TGF beta 1 after stimulation with immobilized monoclonal antibodies directed against CD 3 and CD 28 ( p=0 . 014 ) . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
CD 28 driven recovery of CD 4 cells was superior to that of CD 8 T cells . ^^^ CD 28 superagonist expanded CD 4 T cells had maintained repertoire diversity and were functional both in vitro and in vivo , suggesting that treatment with a human CD 28 specific superagonist will protect T lymphopenic patients from opportunistic infections . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
This study investigated coexpression of CD 28 , CD45RA , and CD45RO on CD 4 ( + ) and CD 8 ( + ) cells in 107 human immunodeficiency virus ( HIV ) type 1 infected patients , who were followed up prospectively and were not treated with highly active antiretroviral therapy , and 65 control subjects . ^^^ The most important novel finding was that a 50 % reduction in CD 4 ( + ) CD 28 ( + ) cells predicted increased mortality ( relative hazards [ HR ] , 1 . 6 ; 95 % confidence interval [ CI ] , 1 . 0 2 . 6 ; P= . 04 ) , even after adjusting for the CD 4 ( + ) cell counts , virus load , beta ( 2 ) microglobulin and hemoglobin levels , and HIV disease stage . ^^^ Concerning the proportions of cells , only CD 4 ( + ) CD 28 ( + ) , CD 4 ( + ) CD45RA ( + ) , and CD 8 ( + ) CD45RO ( + ) cells decreased with HIV progression . ^^^ If our results are confirmed in other studies , coexpression of CD 28 on CD 4 ( + ) cells may be a useful marker to evaluate HIV progression . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
This defect was not related to varying proportions of T cell subsets ( e . g . , CD 4 ( + ) / CD8 ( + ) , CD45RA ( + ) / RO ( + ) , or CD 28 ( ) T cells ) ; 2 ) PKAI activation through the cAMP agonist 8 CPT cAMP markedly inhibited IL 10 secretion from T cells through CD 3 and CD 28 activation in both patients and controls , but the sensitivity for cAMP dependent inhibition was increased in CVID ; 3 ) selective PKAI inhibition by Rp 8 Br cAMPS markedly increased IL 10 secretion in anti CD 3 and anti CD3 / anti CD 28 stimulated T cells in both patients and controls . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In this paper , we report that , in healthy subjects , a subset of CD 8 ( + ) CD 28 ( ) T cells suppresses the specific response to TSH receptor ( TSHR ) of CD 4 ( + ) clones . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Expression of CD 4 , CD8alpha , CD 40 , CD 86 , CD 28 , CD 31 and vascular endothelial growth factor was not different between the two tumor groups but expression of CD 40 ligand , CD 80 , NK1 . 1 and CXCR 3 was relatively lower in A11 / Mig tumors . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
An effective humoral response requires bidirectional interactions between B cells and CD 4 ( + ) T cells , critical of which are interactions between CD80 / CD86 expressed on activated B cells and CD 28 expressed on responder CD 4 ( + ) T cells . ^^^ The importance of interactions of CD80 / CD86 and CD 28 in activating CD 4 ( + ) T cells was clear ; the ablation of a normal response following the addition of neutralizing anti CD86 / CD80 Abs mirrored the response of CD 4 ( + ) T cells to B cells of HIV viremic patients , while the addition of exogenous CD 28 ligands partially restored the poor CD 4 ( + ) T cell response to the B cells of HIV viremic patients . ^^^ Our findings further delineate the scope of defects associated with cognate B cell CD 4 ( + ) T cell interactions in HIV infection and suggest that therapeutic interventions designed to enhance CD 28 dependent costimulatory pathways may help restore immune functions . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
We found that CD 4 ( + ) CD 8 ( + ) T cells expressed the costimulatory CD 28 molecule less and were more prone to die in vitro after phytohemagglutinin / interleukin 2 stimulation than were CD 4 ( + ) T cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Our results obtained from qualitative reverse transcriptase polymerase chain reaction and quantitative flow cytometry analyses revealed that the CD 4 ( + ) CD 25 ( + ) T cells constitutively express HO 1 and that T cell stimulation with plate bound anti CD 3 in combination with soluble anti CD 28 not only induced HO 1 gene expression in the CD 4 ( + ) CD 25 ( ) T cells but also up regulated HO 1 gene expression in the CD 4 ( + ) CD 25 ( + ) T cells . ^^^ Our further studies showed that CD 28 signal alone was enough to induce HO 1 expression and CD 3 signal , of which signal alone did not induce HO 1 expression , was required at least for full HO 1 expression in both CD 25 ( ) and CD 25 ( + ) subsets of human CD 4 ( + ) T cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
CD 28 and inducible costimulatory protein Src homology 2 binding domains show distinct regulation of phosphatidylinositol 3 kinase , Bcl xL , and IL 2 expression in primary human CD 4 T lymphocytes . ^^^ To examine how these differences in signal transduction affected IL 2 production , we transduced primary human CD 4 T cells with a lentiviral vector that expressed the murine CD 28 extracellular domain with a variety of human CD 28 and ICOS cytoplasmic domain swap constructs . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The Nef proteins of human immunodeficiency virus and simian immunodeficiency virus ( SIV ) bind the AP 1 and AP 2 clathrin adaptors to downmodulate the expression of CD 4 and CD 28 by recruiting them to sites of AP 2 clathrin dependent endocytosis . ^^^ Surprisingly , deletion of constitutively strong AP 2 binding determinants ( CAIDs ) in SIV Nef had little effect on its ability to induce TCR CD 3 , or 8 zeta endocytosis , even though these determinants are required for the induction of CD 4 and CD 28 endocytosis via this pathway . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Nef directly interacts with components of the cellular machinery involved in the vesicular transport between the endocytic compartments , mainly the clathrin adaptor complexes ( AP ) , inducing the misrouting of numerous cellular proteins , including CD 4 , MHC 1 , LIGHT , DC SIGN , CD 28 and MHC 2 to the endosomal degradation compartment or the trans Golgi network . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
To address their migratory properties of CD 4 ( + ) CD 28 ( ) T cells we studied the expression of the inducible inflammatory Th 1 type chemokine receptor CCR 5 in localized WG and generalized WG . ^^^ Expansion of CD 4 ( + ) CD 28 ( ) T cells was more prominent in generalized WG compared to localized WG . ^^^ In localized WG a larger fraction of CD 4 ( + ) CD 28 ( ) T cells displayed CCR 5 expression compared to generalized WG . ^^^ Higher levels of CCR 5 expression on CD 4 ( + ) CD 28 ( ) T cells in localized WG may favor stronger CCR 5 mediated recruitment of this T cell subset into granulomatous lesions in localized WG . ^^^ Expansion of Th 1 type CD 4 ( + ) CD 28 ( ) CCR 5 ( + ) effector memory T cells might contribute to disease progression and autoreactivity , either directly , by maintaining the inflammatory response , or as a result of bystander activation . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Our results show that sharp differences exist between the CD 8 and CD 4 T cell subsets in ( 1 ) cell cycle programs ( as assessed by both in vitro proliferation and in vivo turnover measurement ) ; ( 2 ) CD 28 regulation on cell cycle entry ; and ( 3 ) accumulation of immediate effector cells among the CD 28 cells , believed to be close to or at replicative senescence . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Monoclonal CD 4 ( + ) T LGLshowed expression of TCRalphabeta , variable levels of CD 8 ( CD 8 ( / +dim ) ) and a homogeneous typical cytotoxic ( granzyme B ( + ) , CD 56 ( + ) , CD 57 ( + ) , CD11b ( + / ) ) and activated / memory T cell ( CD 2 ( +bright ) , CD 7 ( / +dim ) , CD11a ( +bright ) , CD 28 ( ) , CD62L ( ) HLA DR ( + ) ) immunophenotype . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Melanoma patients with thinner tumors and those surviving revealed an increase of CD 19 ( + ) CD 80 ( + ) and CD 19 ( + ) CD 80 ( + ) CD 86 ( + ) cells , as well as a higher concentration of CD 3 ( + ) CD 4 ( + ) CD 28 ( + ) cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In the second system , functional differentiation was induced in purified naive CD 4 T cells from DO 11 . 10 TCR transgenic and RAG 2 deficient mice with cytokines and antibodies to CD 3 and CD 28 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
A stringent requirement for CD 28 costimulation was not evident in interactions between transitional B cells and preactivated CD 4 expressing T cells , indicating that T cells involved in vivo in an ongoing immune response might rescue Ag specific transitional B cells from negative selection . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The resulting congenic strain , B6 . kd , was mated with partners homozygous for targeted mutations of CD 4 , CD 8 , CD 28 , IL 2 , recombinase activating gene 1 ( Rag 1 ) , ICAM 1 , or beta ( 2 ) microglobulin . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Likewise , CD 4 T lymphocyte expression of the co stimulatory molecule CD 28 was also an independent predictor of response to immunization ( rho = 0 . 5 ; P < 0 . 005 ) . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
These CD40L ( + ) T cells were CD 4 ( + ) , CD 69 ( + ) , but negative for CD 8 , CD 25 , CD 28 , and ICOS . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Immunohistological single and double staining ( PAP / APAAP ) with monoclonal antibodies against HLA I / II , CD 4 , CD 8 , CD 28 , B 7 1 , B 7 2 , LFA 1 and CD 68 were performed in tissue samples from patients with crohn ' s disease ( n = 15 ) , ulcerative colitis ( n = 14 ) , colorectal carcinoma ( n = 5 ) and FAP ( n = 3 ) . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Changes in total and memory CD 4 and CD 8 cells were similar in the groups , as were decreases in immune activation ( e . g . , CD 38 and HLA DR ) and increases in CD 28 expression . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
These studies reveal that deletion is efficient in reconstituted radiation chimeras and is B cell , CD 28 , inducible costimulatory molecule , Fas , CD 4 , and CD 8 independent . ^^^ In contrast , TCR revision is radiosensitive , B cell , CD 28 , and inducible costimulatory molecule dependent , Fas and CD 4 influenced , and CD 8 independent . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Ligation of CD 28 , which is present on the majority of CD 4 ( + ) T cells , promotes proliferation and immune responses . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Moreover , such mice had increased numbers of CD 4 ( + ) sdLN cells that were CD 25 ( + ) , CD 28 ( + ) or CD 152 ( + ) and accessory cells that were CD 40 ( + ) or MHC 2 ( + ) . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Enriched CD 4 ( + ) T cells were cultured with or without antibodies against CD 3 and CD 28 T cell receptors and with irradiated peripheral blood mononuclear cells from HIV 1 seronegative individuals . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
CD 7 and CD 28 single deficient and CD7 / CD28 double deficient mice had normal levels of CD 4 and CD 8 single positive T cells in thymus and spleen . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
There was a marked reduction , particular in advanced stage MM , in the proportion of CD 4 and CD 8 cells expressing CD 28 , CD 152 , CD3zeta , p56lck , ZAP 70 and PI 3 k . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
To identify these differences , CD 4 ( + ) T cells were isolated from human cord blood , polarized in vitro to Th 1 and Th 2 and activated via CD 3 and CD 28 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
CD 4 T cells retained the ability to express a costimulatory molecule , CTLA 4 , but showed a decrease in CD 28 expression . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
We used a colitis model in which disease causing CD45RB ( hi ) T cells alone or in combination with CD 4 ( + ) CD 25 ( + ) T cells from either CD 28 deficient or wild type donors were transferred into T cell deficient animals , half of which were treated with ICOS blocking reagents . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In addition , after HAART , HIV 1 specific CD 4 ( + ) T cells are enriched for CD 27 ( + ) CD 28 ( ) expressing cells , a rare phenotype , reflecting an early intermediate stage of differentiation . ^^^ The role for HIV 1 specific CD 4 ( + ) T cells expressing CD 27 ( + ) CD 28 ( ) after HAART remains to be determined . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The in vivo differentiation / survival of CD 4 ( + ) CD 25 ( + ) T suppressor cells is dependent on IL 2 and CD 28 mediated costimulatory signals . ^^^ Inhibition of the interaction of CD 28 or cytotoxic T lymphocyte associated antigen 4 ( CTLA 4 ) with CD80 / CD86 in the pre culture of CD 4 ( + ) CD 25 ( + ) cells did not prevent the induction of suppressor function . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Studies showing an expansion of circulating tumor necrosis factor ( TNF ) alpha producing Th 1 type CD 4 ( + ) CD 28 ( ) T cell effector memory T cells and their presence as Th 1 type cytokine profile driving cell population within granulomatous lesions provide the rationale for using TNF alpha blocking agents in Wegener ' s granulomatosis refractory to standard induction therapy with cyclophosphamide and corticosteroids ( `` Fauci ' s scheme ' ' ) . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The secondary endpoints were renal function ; histological parameters related to CyA ; serum level of TGF beta , PDGF BB , blockade of CD 25 molecule and surface expression of CD 3 , CD 4 , CD 8 , CD 69 , CD11a , CD49d , CD 28 , CD 152 molecules in the subpopulations of T cells in the peripheral blood . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
DESIGN : In 35 patients with pulmonary tuberculosis ( PTB ) and their contacts , 29 persons with tuberculin skin test conversion ( TSTC ) and 20 healthy individuals with negative tuberculin skin test ( NTST ) , we studied T lymphocyte subsets ( CD 3 , CD 4 , CD 8 , alphabetaTCR and gammadeltaTCR ) , B cells , monocytes and co stimulatory molecules CD 28 and CD 86 in peripheral blood . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
CD 28 costimulation enhances the sensitivity of the ELISPOT assay for detection of antigen specific memory effector CD 4 and CD 8 cell populations in human diseases . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
CD 28 costimulation is essential for CD 4 ( + ) T cell proliferation , survival , interleukin 2 ( IL 2 ) production and T helper type 2 development . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Stimulation via CD 40 is an important component of this CD 4 T cell help , and interestingly , some of its effects appear to be independent of CD 28 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Our data show that CD 4 T cell B cell cooperation is dependent on CD 28 but not PKCtheta , whereas CD 28 costimulatory signals that augment proliferation can be uncoupled from signals that regulate anergy . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
An increased percentage of memory CD 4 ( + ) CD45RO ( + ) T cells lacking the expression of costimulatory receptor CD 28 was detected in patients with active CD when compared to the other groups evaluated . ^^^ This expanded CD 4 ( + ) CD45RO ( + ) CD 28 ( null ) T cell subset expressed mostly the effector cell marker CD 57 ( + ) . ^^^ Moreover , sorted CD 4 ( + ) CD45RO ( + ) and CD 4 ( + ) CD45RO ( + ) CD 28 ( null ) T cells from patients with active CD exhibited a lower apoptotic rate than that found in healthy controls and inactive CD patients . ^^^ According to our data , circulating T lymphocytes from active CD patients show distinctive phenotypic and functional changes , characterized by an expansion of memory CD 4 ( + ) CD45RO ( + ) CD 28 ( null ) T cells expressing effector associated cell surface molecules and displaying enhanced resistance to apoptosis . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
IL 1 acts on antigen presenting cells to enhance the in vivo proliferation of antigen stimulated naive CD 4 T cells via a CD 28 dependent mechanism that does not involve increased expression of CD 28 ligands . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
We show that age dependent reduction in effective precursor numbers depends on the one hand , extensive , activation dependent apoptosis occurring from the very onset of stimulation and , on the other , the accumulation of non dividing , regulatory ( suppressor ) CD 4 ( lo ) CD 25 ( + ) CD 28 ( lo ) T cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
RESULTS : HR 8348 cell line did not express CD 2 , CD 3 , CD 4 , CD 5 , CD 7 , CD 8 , TCR , CD 10 , CD11b , CD 14 , CD 16 , CD 19 , CD 22 , CD 25 , CD 28 , SmIg , CD 33 , CD 35 , CD 36 , CD41a , CD 45 , CD45RA , CD45RO , CD 56 , CD 61 , CD 64 , CD66b , CD 69 , CD 71 , CD 117 , CD 122 and P glycoprotein but expressed CD 13 , CD 15 , CD 20 , CD 38 , CD 95 and HLA DR . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
As a group , the seven who lost anti HIV CTL were older , had weaker baseline anti HIV CTL activity , higher historical virus loads , lower historical and contemporary CD 4 T cell counts and a lower percentage of CD 8 T cells expressing CD 28 . ^^^ Multivariate analysis suggested that CD 4 T cell counts and anti HIV CTL amplitude at study onset were independently associated with CTL loss in these individuals , as was percentage of CD 8 T cells expressing CD 28 at study ' s end . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
DESIGN : T cells are a heterogeneous mix of naive and memory subsets delineated by simultaneously measuring CD 4 , CD 8 , CD45RA / RO , CD11a , CD 28 , and CD 27 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Using aAPC that have been transfected with CD137L ( 4 1BBL ) and CD 32 ( FcRgammaII ) , as a means to bind anti CD 3 and anti CD 28 antibody , we found that CD 4 cells preferentially expanded in vitro with K 562 aAPC , while CD 8 cells expanded with both K 562 and YAC 1 aAPC . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The measurements of CD 4 ( + ) and CD 8 ( + ) T lymphocyte subsets ( CD45RA , CD45RACD62L , CD45RO , CD 28 , CD 38 ) were carried out by flow cytometry . ^^^ By 6 months , higher slopes were observed in full versus partial responders in the % of CD 28 on CD 4 ( + ) and the % of CD 4 ( + ) memory subset and in both nave and memory CD 4 ( + ) subsets from 6 to 42 months . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Restimulated CD 4 ( + ) DKO T cells that had been primed in vivo showed decreased proliferation and reduced IL 4 and IL 10 production compared with restimulated CD 4 ( + ) T cells from CD 28 KO mice . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
METHODS : Thymocytes were freshly prepared and were cultured for 20 h in the presence of anti TCRalphabeta mAb+anti CD 28 mAb or anti TCRalphabeta mAb along , The cultured cells were stained with fluorescein labelled Annexin 5 , PI , anti CD 4 mAb and anti CD 8 mAb , then color reagents . ^^^ RESULTS : Compared with the spontaneous apoptosis of thymocytes cultured in medium alone , CD 28 costimulator markedly enhanced the number of thymocyte apoptosis at early and intermediate phases ; under the action of double signaling stimulators , the apoptosis of CD 4 ( + ) CD 8 ( + ) ( DP ) thymocytes were substantially increased , and the expression of CD 28 were also upregulated on these apoptotic DP cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
We previously reported that the CD 28 ( ) CD 4 ( + ) T cell subpopulation was expanded in the kidney allograft patients with long graft survival , although these T cells were rarely found in patients with graft survival < 5 yr . ^^^ To understand the CD 28 ( ) CD 4 ( + ) T cells in the long term acceptance of kidney allografts , we examined functions of this population and performed a 4 yr follow up study . ^^^ CD 28 ( + ) CD 4 ( + ) and CD 28 ( ) CD 4 ( + ) T cells purified by cell sorting were analyzed for expression of 5 ( beta ) repertoire . ^^^ A follow up study with long term kidney allograft patients was performed for 4 yr about the rate of CD 28 ( ) CD 4 ( + ) T cells . ^^^ Four patients with a marked increase of CD 28 ( ) CD 4 ( + ) T cells showed the donor specific responses appeared to be lower when compared with third party specific responses . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
After in vitro culture for 3 12 wk , the flu specific CD 4 T ( CM ) had surface markers that were generally consistent with an effector phenotype described for CD 8 T cells , except for the maintenance of CD 28 expression . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The changes in the expression of CD 3 , CD 5 , CD 8 , CD 4 , CD 28 , CD 56 , and CD 19 were monitored in highly purified lymphocyte populations obtained by negative selection in a fluorescence activated cell sorter . ^^^ The kinetics of expression loss was rapid for CD 8 , CD 56 , and CD 28 , and more than 80 % of initial expression was lost in the early stages of apoptosis but was slower for CD 3 , CD 5 , and CD 4 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The expressions of single staining CD 3 , CD 4 , and CD 8 , as well as double staining CD 4 CD25 , CD 3 CD56 and CD 28 CD95 on CIK cells were analyzed by flow cytomctry on 3 , 6 , 12 , 24 and 30 days of co culture . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
METHODS : In two follow up surveys of the SARS patients discharged from our hospital , the changes of CD 3 ( + ) , CD 4 ( + ) , CD 8 ( + ) , CD 8 ( + ) CD 28 ( + ) , CD 8 ( + ) CD 28 ( ) , CD 3 ( + ) CD 25 ( + ) , CD 3 ( + ) CD 69 ( + ) , CD 3 ( + ) HLA DR ( + ) T cells and the ratio of CD 4 ( + ) / CD8 ( + ) T cells in peripheral blood of SARS patients treated unitedly with Chinese and Western medicines were detected by flow cytometry . ^^^ RESULTS : In two follow up surveys , the percentages of CD 3 ( + ) , CD 4 ( + ) , CD 8 ( + ) CD 28 ( + ) T cells and the ratio of CD 4 ( + ) / CD8 ( + ) T cells were remarkably lower than those of normal values ( P < 0 . 05 ) , while the percentage of CD 8 ( + ) CD 28 ( ) T cells was higher ( P < 0 . 01 ) . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In the present study we demonstrate that SIVcpz and HIV 1 group N or O nef alleles down modulate CD 4 , CD 28 , and class 1 or 2 MHC molecules and up regulate surface expression of the invariant chain ( 2 ) associated with immature major histocompatibility complex ( MHC ) class 2 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Furthermore , blocking OX 40 costimulation induced long term skin allograft survival in CD 4 deficient mice and CD 8 deficient mice when both CD 28 and CD 154 were transiently blocked . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Consistent with this hypothesis , T cells from CD11b KO and CD11d KO splenocytes exhibited reduced intensity of CD 3 and CD 28 expression and decreased ratios of CD4 / CD8 cells , and CD 4 ( + ) T cells were reduced among CD11b KO and CD11d KO thymocytes . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
B 7 CD 28 interaction promotes proliferation and survival but suppresses differentiation of CD 4 CD8 T cells in the thymus . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Antigen specific CD 8 ( + ) CD 28 ( ) and CD 4 ( + ) CD 25 ( + ) T suppressor / regulatory cells induce the upregulation of inhibitory receptors expressed by antigen presenting cells ( APC ) belonging to the family of immunoglobulin like transcripts ( ILTs ) and downregulation of costimulatory molecules in APC . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Although CD 8 ( + ) CD 28 ( ) suppressor T cells , apoptotic cells and co stimulatory molecules were not altered , CD 4 ( + ) T cells expressing interleukin ( IL ) 10 were augmented in rats receiving CD8alpha ( + ) DC compared to rats receiving total DC or medium . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
A role for CD 28 in lymphopenia induced proliferation of CD 4 T cells . ^^^ In this study , we show that accumulation of highly divided CD 4 T cells characterized by great potential to make IFN gamma is significantly delayed in the absence of B 7 : CD 28 costimulation during LIP . ^^^ Furthermore , CD 28 sufficient CD 4 T cells show great competitive advantage over CD 28 deficient CD 4 T cells when transferred together into the same lymphopenic hosts . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
AIM : To explore the role of 4 1BB molecule in the activation and proliferation of CD 4 ( + ) T cells and CD 8 ( + ) T cells and compare it with that of CD 28 molecule . ^^^ The costimulatory signal mediated by 4 1BB molecule could mainly induce the proliferative response of CD 8 ( + ) T cells , while that mediated by CD 28 molecule could induce the activation of CD 4 ( + ) T cells mainly . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The effective deletion of alloreactive T helper and cytotoxic cells in conjunction with the expansion of antigen specific suppressor ( CD 8 + CD 28 FOXP3+ ) and regulatory ( CD 4 + CD25+ FOXP3+ ) T cells creates a milieu in which the graft is well tolerated under an `` umbrella ' ' of low dosage immunosuppression . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Peripheral blood samples were taken on days 1 through 28 after transplantation , and the lymphocyte fractions were exposed to the monoclonal antibodies anti CD 3 , CD 19 , CD 4 , CD 8 and CD 28 for a flow cytometer analysis . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) cells display CD45RO ( high ) , CD45RA ( low ) , CD 28 ( high ) , CD62L ( high ) , and CD 95 ( high ) phenotype . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In contrast with regular KIR ( ) CD 4 T cells , the majority of KIR ( + ) CD 4 T cells lacked surface expression of CD 27 , CD 28 , CCR 4 , and CCR 7 , but did express CD 57 and 2B4 . ^^^ In addition , KIR were detected on approximately one tenth of CD 28 ( ) and CD 57 ( + ) memory CD 4 T cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
These observations indicate that both type 2 CD 4 and CD 8 T cell polarizations are efficiently induced via costimulation of CD 28 with its natural ligands , although the differentiation of CD8+ T cells is dependent on CD4+ cells . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Tolerized ILT 3 ( high ) , ILT 4 ( high ) APC anergize CD 4 ( + ) T ( H ) cells and can induce the generation of antigen specific CD 4 ( + ) CD 25 ( + ) T regulatory cells ( T ( R ) ) cells and CD 8 ( + ) CD 28 ( ) T ( S ) cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
RESULTS : Peripheral blood lymphocytes from patients with long term surviving kidneys had decreased expression of T cell receptor ( TCR ) alphabeta ( P < 0 . 01 ) , CD3epsilon ( P < 0 . 05 ) , and CD 3 zeta chains ( P < 0 . 001 ) ; diminished percentages of CD 4 ( + ) CD 28 ( + ) ( P < 0 . 001 ) cells ; and increased expression of CTLA 4 ( + ) ( P < 0 . 01 ) on CD 3 ( + ) cells . ^^^ The decrease in the percentage of CD 28 ( + ) cells and the increase in CD 4 ( + ) CD 25 ( + ) CD 69 ( + ) cells suggest that regulatory mechanisms of the immune response are still active in such patients . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The number of positive cell for CD 3 , CD 4 , CD 8 , CD 25 , CD 28 , CTLA 4 ( CD 152 ) , Fas , FasL , bcl 2 , bcl 10 were determined using immunohistochemical staining techniques . ^^^ The number of CD 4 , CD 28 , FasL , CTLA 4 and bcl 10 positive cells was higher in adenoids allergic than non allergic children . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
A higher proviral DNA level was associated with increases in CD 4 ( + ) CD45RA ( ) CD 28 ( ) effector cells and reductions in naive CD 4 ( + ) CD45RA ( + ) CD62L ( + ) and CD 8 ( + ) CD45RA ( + ) CD62L ( + ) cells ( P < . 05 ) . ^^^ Higher levels of activated CD 4 ( + ) HLA DR ( + ) and early differentiated CD 4 ( + ) CD45RA ( ) CD 28 ( + ) cells predicted increased risk of subsequent detectable viremia in patients with undetectable HIV RNA ( P < . 05 ) . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
DQ 8 mice deficient for CD 28 had comparable numbers of CD 4 and CD 8 T cells as DQ8 . ^^^ We observed an increase in CD 4 ( + ) CD 28 ( ) NKG2D ( + ) cells after immunization , suggesting an important role for cells bearing this receptor in the disease process . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Our laboratory has demonstrated that down regulation of proliferation and cytokine synthesis by CD 4 ( + ) T cells in mice fed diets rich in n 3 polyunsaturated fatty acids ( PUFA ) is highly dependent on the involvement of the co stimulatory molecule , CD 28 . ^^^ The proliferation of purified splenic CD 4 ( + ) T cells activated in vitro with anti CD 3 and anti CD 28 ( alphaCD3 / CD28 ) from conventional mice ( C57BL / 6 ) fed either a control corn oil ( CO ) enriched diet devoid of n 3 PUFA , docosahexaenoic acid ( DHA ; 22 : 6 ) or eicosapentaenoic acid ( EPA ; 20 : 5 ) for 14 days was suppressed by dietary DHA and EPA . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Finally , increased selection of mature CD 4 and CD 8 SP T cells was observed in non TCR Tg CD28KO and B7DKO mice , indicating that this function of CD 28 B7 interaction is not unique to a TCR Tg model . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Sections were stained with antibodies against : EG 2 ( eosinophils ) , CD 4 ( T cells ) , CD 68 ( macrophages ) , CD1a ( Langerhans cells ) , CD 28 ( on T cells ) and costimulatory molecules ( CD 80 , CD 86 ) . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
ATG induced a persistent decrease of peripheral blood lymphocyte counts compared with non ATG treatment because of a predominant decrease of CD4+ T cells ( 4 months , 1 year ; P < 0 . 0005 ) which was associated with a decreased CD 28 expression ( 1 year , P = 0 . 02 ) and CD 4 cell interleukin 2 ( IL 2 ) response ( 4 months , P < 0 . 0005 ) . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
We report that deficiency for p 27 ( Kip 1 ) has a more pronounced effect on the expansion of murine naive CD 8 ( + ) T cells and that this disparity is due to a reduced requirement for CD 28 mediated costimulation in CD 8 ( + ) but not CD 4 ( + ) T cells lacking p 27 ( Kip 1 ) . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
A concomitant increase in levels of CD 4 ( + ) DR ( + ) , CD 8 ( + ) DR ( + ) , and CD 8 ( + ) CD 28 ( ) cells was detected . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Using CD 4 or CD 28 deficient murine T cells , it was suggested that recruitment of Lck to the IS depends on CD 4 , whereas CD 28 sustains Lck activation . ^^^ Thus , we performed a series of experiments to determine 1 ) whether Lck is recruited to the IS through lipid rafts ; and 2 ) whether Lck recruitment to the IS of human resting T cells depends on CD 4 or on CD 28 engagement . ^^^ We found that CD 28 , but not CD 4 , stimulation induced recruitment of Lck into detergent resistant domains as well as its accumulation at the IS . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The expression and functional roles of costimulatory receptors , CD 28 and cytotoxic T lymphocyte antigen 4 ( CTLA 4 ) , and their ligands , CD 80 and CD 86 , on primary mouse CD 4 ( + ) T cells after activation with different doses of Con A were studied . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Senescence of effector CD 4 ( + ) T cells is associated with the loss of CD 28 and the de novo expression of KIR2DS2 , NKG2D , and CX ( 3 ) CR 1 , all of which function as costimulatory molecules and reduce the threshold for T cell activation . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
These include major histocompatibility complex 1 ( MHC 1 ) and ( MHC 2 ) present on antigen presenting cells ( APCs ) and target cells , and CD 4 and CD 28 present on helper T cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Antigen presenting cells ( APCs ) initiate activation of CD 4 ( + ) T cells in a multistep process that minimally involves co ligation of the TCR and CD 4 by the MHC class II / peptide complex and costimulation through additional T cell surface molecules such as CD 28 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Mab 2 4 , which recognizes the chicken CD 28 molecule , was found to react with CD 4 positive duck lymphocytes and with CD 8 positive duck T cells but not with CD 8 positive B cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
HIV may produce inhibitory microRNAs ( miRNAs ) that block production of CD 28 , CD 4 and some interleukins . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
This study examined the role of these inhibitory pathways in regulating CD 28 dependent and CD 28 independent CD 4 and CD 8 alloreactive T cells in vivo . ^^^ The same treatment led to prompt rejection in CD 28 ( / ) and a smaller proportion of CD 4 ( / ) CD 28 ( / ) mice with no effect in CD 8 ( / ) CD 28 ( / ) recipients . ^^^ Thus , the PD 1 : PD L 1 pathway down regulates alloreactive CD 4 T cells , particularly in the absence of CD 28 costimulation . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In vitro CD 8 ( + ) CD 28 ( ) CD 56 ( + ) T cells tolerize APCs , prevent the priming of naive CD 4 ( + ) T cells , and suppress memory CD 4 ( + ) T cell responses . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Mice lacking CTLA 4 , which show spontaneous CD 28 mediated CD 4 ( + ) T cell activation , expansion and differentiation , were treated with antagonistic alphaICOS antibodies . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In addition , we demonstrate that statins reduce cell surface expression of other immunoregulatory molecules , which include MHC 1 , CD 3 , CD 4 , CD 8 , CD 28 , CD 40 , CD 80 , CD 86 , and CD 54 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Dysregulation of CD 28 and CTLA 4 expression by CD 4 T cells from previously immunodeficient HIV infected patients with sustained virological responses to highly active antiretroviral therapy . ^^^ METHODS : We examined expression of CD 28 , cytotoxic T lymphocyte antigen 4 ( CTLA 4 ) and intracellular perforin by CD 4 and CD 8 lymphocytes from 25 highly selected HIV infected patients [ nadir CD 4 T cell counts < 50 cells / microL , > 4 years on HAART and > 6 months of complete viral suppression ( < 50 HIV 1 RNA copies / mL ) ] and 18 HIV seronegative age and sex matched controls . ^^^ RESULTS : HIV infected patients had lower percentages of CD 28 expressing CD 4 lymphocytes and higher percentages of CTLA 4 expressing CD 4 lymphocytes than controls . ^^^ The percentage of CTLA 4 expressing CD 4 lymphocytes correlated inversely with that of CD 28 expressing CD 4 lymphocytes . ^^^ CONCLUSIONS : Expression of CD 28 , CTLA 4 and perforin by CD 4 lymphocytes remain dysregulated in HIV infected patients with previous severe immunodeficiency , despite increased CD 4 T cell counts and control of HIV viraemia by HAART . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Addressing this , we demonstrate that , similarly to the SIVmac 239 nef , primary SIVsm and HIV 2 nef alleles down modulate cell surface expression of human CD 4 , CD 28 , CD 3 , and class 1 or 2 major histocompatibility complex ( MHC 1 or MHC 2 , respectively ) molecules , up regulate surface expression of the invariant chain ( 2 ) associated with immature MHC 2 , inhibit early T cell activation events , and enhance virion infectivity . ^^^ Mutational analysis showed that a dileucine based sorting motif in the C proximal loop of SIV or HIV 2 Nef is critical for its effects on CD 4 , CD 28 , and 2 but dispensable for down regulation of CD 3 , MHC 1 , and MHC 2 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Nef proteins of primate lentiviruses promote viral replication , virion infectivity , and evasion of antiviral immune responses by modulating signal transduction pathways and downregulating expression of receptors at the cell surface that are important for efficient antigen specific responses , such as CD 4 , CD 28 , T cell antigen receptor , and class 1 and class 2 major histocompatibility complex . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Human immunodeficiency virus ( HIV ) Nef is a membrane associated protein decreasing surface expression of CD 4 , CD 28 , and major histocompatibility complex class 1 on infected cells . ^^^ The Nef mediated internalization of receptors , such as CD 4 , major histocompatibility complex class 1 , CD 28 , and CD8alphabeta , may contribute to the subversion of the host immune system and progression towards AIDS . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Our data indicate that patients with RRMS , treated with IFN beta1a , exhibited a significant increase in CD 4 ( + ) CD 25 ( ++ ) T suppressor cells , accompanied by a significant decrease in cytotoxic lymphocytes ( CD 8 ( + ) CD 28 ( ) and natural killer [ NK ] cells ) and IFN gamma production , which could both contribute to an explanation of the previously described beneficial effects of IFN beta treatment in MS . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Disease progression in WG is accompanied by a profound generalized alteration of T cell differentiation with an increase of effector memory T cells ( CD 4 ( + ) CD 28 ( ) ) . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The percentage of CD 4 ( + ) CD 28 ( null ) and cytotoxicity of CD 4 T lymphocytes were also significantly higher in OSA patients than in controls . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Immunophenotyping of tumor cells revealed massive infiltration of lesions by CD 4 ( + ) T cells , which express CD 28 , CD 69 , and interleukin 4 but not interferon gamma , suggesting that tumor B cell proliferation was driven by Th 2 polarized , immunocompetent , and activated T cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
CD 28 is constitutively expressed on CD 4 ( + ) cells , but its homologue CD 152 is only weakly expressed after cell activation . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
By contrast , HGMC did not express detectable amounts of CD 1 , CD 2 , CD 4 , CD 5 , CD 14 , CD 15 , CD 16 , CD 22 , CD 24 , CD 25 , CD 26 , CD 27 , CD 28 , CD 31 , CD 32 , CD 34 , CD 35 , CD 88 , or CD 116 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
RESULTS : CD 4 ( + ) CD 28 ( ) T cells had higher ratios of CXCR 3 to CCR 4 than CD 4 ( + ) CD 28 ( + ) T cells . ^^^ Both , CD 4 ( + ) and CD 8 ( + ) CD 28 ( ) T cells of patients with AS produced more IFNgamma , TNFalpha , and IL 10 than their CD 28 ( + ) counterparts ( p < 0 . 05 ) , and lacked the production of IL 2 and IL 4 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
We have demonstrated that downregulation of proliferation by CD 4 ( + ) T cells in mice fed n 3 PUFA diets is dependent on the involvement of CD 28 . ^^^ The proliferation of splenic CD 4 ( + ) T cells was suppressed by DHA and EPA following stimulation with anti CD 3 and anti CD 28 . ^^^ Surprisingly , the number of surface CD 28 molecules was not reduced in activated CD 4 ( + ) T cells from either group of n 3 PUFA fed mice . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Disruption of CD 28 costimulation reduced the number of type 1 CD 4 and CD 8 cells generated and impaired resistance to infection . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In conclusion , we detected the presence of cells expressing the markers for Tregs ( CD 4 ( + ) CD 25 ( + ) ) and suppressor ( CD 8 ( + ) CD 28 ( ) ) in the tumour microenvironment . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The presence of HIV specific CD 4 ( + ) IL 2 ( + ) T cells was associated with low levels of proliferating T cells within the less differentiated T cell subpopulations ( defined by CD45RA , CCR 7 , CD 27 , and CD 28 ) . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Loss of IL 7Ralpha is associated with CD 4 T cell depletion , high interleukin 7 levels and CD 28 down regulation in HIV infected patients . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The cells were labeled with conjugated antibodies specific for CD 4 , CD 8 , CD 28 , CD45RO , and Fas , and were analyzed by FACS ( ( R ) ) . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Ligation of CD 28 by its natural ligand CD 86 in the absence of TCR stimulation induces lipid raft polarization in human CD 4 T cells . ^^^ Using an Ig fusion with the extracellular domain of CD 86 ( CD86Ig ) bound to a magnetic bead or K 562 cells expressing CD 86 , we demonstrated that ligation of CD 28 by its natural ligand , but not by Ab , induced polarization of LRs at the cell bead interface of fresh human CD 4 T cells in the absence of TCR ligation . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Adoptive transfer of CD4+ T cells from wild type , CD 28 ( / ) , CD40L ( / ) , or IFN gamma ( / ) donors to CD 4 ( / ) recipients delineated functions of these CD4+ T cell expressed molecules on the outcome of infection . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In PBC , numbers of CD 4 ( + ) CD 28 ( ) bIEL , which are reportedly responsible for target tissue destruction in autoimmune diseases , were markedly increased in damaged interlobular ducts . ^^^ Increased CD 4 ( + ) CD 28 ( ) bIEL in damaged bile ducts of PBC may be related to immune mediated biliary damage . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Differential distribution of CD 4 ( + ) CD 25 ( bright ) and CD 8 ( + ) CD 28 ( ) T cells in decidua and maternal blood during human pregnancy . ^^^ The aim of this study was to determine the distribution of specific T cell subsets in d . basalis and d . parietalis in early and term pregnancy , with a special emphasis on the presence of CD 4 ( + ) CD 25 ( bright ) ( regulatory ) T cells and CD 8 ( + ) CD 28 ( ) ( suppressor ) T cells . ^^^ We identified significantly higher percentages of CD 4 ( + ) CD 25 ( bright ) and CD 8 ( + ) CD 28 ( ) T cells in decidua compared to peripheral blood suggesting an important role for these T cell subsets locally at the fetal maternal interface . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
CD 4 T cells in the senescence program were identified by the loss of CD 28 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In recent cellular profiling studies , increased levels of a proinflammatory T cell subset ( CD 4 ( + ) CD 28 ( ) ) have been associated with stroke recurrence and death . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In this study , we show that naive wild type or CD 28 ( / ) CD 4 ( + ) CD 25 ( ) T cells exposed to peptide in vivo develop regulatory activity that suppresses the response of adoptively transferred naive T cells to a subsequent immunogenic challenge . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Rainbow trout IELs contain mainly T cells , because they expressed transcripts of T cell marker homologs of CD 8 , CD 4 , CD 28 , CD3epsilon , TCRzeta , TCRgamma , and TCRbeta and lacked IgM . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
CD 4 T cells were stimulated with Ag presented by B 7 negative APC and CD 28 costimulation was provided in trans by anti CD 28 coated beads or by class 2 negative , B 7 positive cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
This mutation inactivates the N distal AP 2 clathrin adaptor binding element and disrupts the abilities of Nef to downregulate CD 4 , CD 28 and CXCR 4 and to stimulate viral replication in vitro . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
It was found that cells with cytotoxic potential such as CD 8 ( + ) CD 28 ( ) T cells , NK and NKT cells as well as lineage ( ) HLA DR ( + ) DC were increased in per cent values , while CD 4 ( + ) T cells and CD 4 : CD 8 ratio were decreased in hepatitis C group . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
For immunohistochemical stainings anti CD 3 , anti CD 19 , anti CD 4 , anti CD 8 , anti CD 25 , anti CD 28 , anti CTLA 4 ( CD 152 ) , anti bcl 2 , anti Fas , and anti FasL antibodies were used . ^^^ RESULTS : The expression of CD 4 , CD 25 , CD 28 , FasL , and CTLA 4 was significantly increased in group 1 compared to group 2 ( p < 0 . 05 ) . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Twenty four hours of TCR engagement and CD 28 costimulation was found sufficient to elicit an optimal rate of cell division over a 72 h period only when a high concentration of IL 2 was produced in the culture and remained readily available to the CD 4 ( + ) T cells . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
However , absence of CD 28 costimulation did not completely impair effector function of CD 4 or CD 8 T cells . ^^^ Antigen specific nave CD 4 and CD 8 T cells , only when activated in the absence of both CD 28 and ICOS pathways , were completely impaired in effector function . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
While CD 28 plays an essential role in the priming of naive CD 4 ( + ) T cells , its contribution to effector memory T cell responses is controversial . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
ICOS , a CD 28 family member expressed on activated CD 4 ( + ) and CD 8 ( + ) T cells , plays important roles in T cell activation and effector function . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
The number of CD 28 ( + ) CD 4 ( + ) peripheral blood mononuclear cells ( PBMC ) in corticosteroid ( CS ) treated patients was comparable to normal control individuals but decreased in untreated AIH patients . ^^^ Analysis of liver infiltrating mononuclear cells ( LIMC ) showed that the number of CD 28 ( + ) CD 4 ( + ) and CD 28 ( ) CD 8 ( + ) LIMC were positively correlated with the histology activity index score . ^^^ Taken collectively , these results suggest that bcl 2 ( + ) CD 28 ( + ) CD 4 ( + ) and bcl 2 ( ) CD 28 ( ) CD 8 ( + ) cells may play critical and distinct roles in hepatocellular damage in AIH . . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In these HIV infected children , when CD 4 ( + ) was depleted , the amount of naive plus central memory CD 8 ( + ) ( CD 28 ( + ) CD 57 ( ) ) cells was decreased , whereas effector CD 8 ( + ) ( CD 28 ( ) CD 57 ( + ) ) cells were upregulated , and these values were always higher than healthy control values . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
During STI cycles , CD 8 ( + ) , CD 8 ( + ) , CD 28 ( + ) , activation markers and naive CD 4 ( + ) T cells increased significantly ( p < 0 . 0001 ) , while both naive CD 8 ( + ) and memory CD 4 ( + ) T cells decreased . ^^^ During DTI , CD 8 ( + ) CD 28 ( + ) T cells fell and CD 4 ( + ) naive T cells stabilized and the rest of the T cell subsets presented changes similar to those during STI cycles . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
In this report , we examined myelin specific CD 8 and CD 4 T cells for two markers differentially expressed on nave , memory and chronically stimulated T cells , CD 28 and CD 57 . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Peripheral blood mononuclear cells were stained for CD 3 , CD 4 , CD 8 , CD 28 , TNF receptor 1 ( TNFRI ) , and TNFRII , and analyzed by quantitative flow cytometry . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
Importantly , as a result of this increased response to TCR stimulation , PTENDeltaT CD 4 ( + ) T cells no longer require CD 28 costimulation for in vitro or in vivo expansion . ^^^
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10747 and P01730	Pubmed	SVM Score :0.0
NA