Pubmed abstracts for Protein-Protein Interaction search result :


Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.60750639
Our data show that CD 71 self associates on the cell surface , that a small fraction of CD 4 can be detected by copurifying it with CD 71 after cross linking , and that the level of association between CD 4 and CD 71 significantly increases after phorbol 12 myristate 13 acetate induced endocytosis of CD 4 . 0.60750639^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Furthermore , these hybrids allowed us to confirm the assignment of the CD 4 antigen , the CD 7 antigen , and the CD 71 antigen to human chromosomes 12 , 17 and 3 , respectively . . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
The effects of tautomycin , a protein phosphatase inhibitor , on recycling of cell surface molecules were studied with transferrin receptor ( TFR ) of human myeloid leukemia K 562 cells and with CD 4 of murine thymocytes . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
T cell antigen expression following PHA stimulation was assessed for helper ( CD 4 ) and suppressor ( CD 8 ) subpopulations : the early T cell activation antigens interleukin 2 receptor ( IL2R ) and transferrin receptor ( TFR ) , and the late antigen ( HLA DR ) using flow cytometry . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Analysis of the changes of surface marker expression of human PBMC during preincubation with CySF L 1 revealed an efficient stimulation of CD 8 and TfR ( transferrin receptor ) expression , partly in conjunction with diminished expression of CD 4 ( T 4 ) . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
To evaluate the modulatory effects of trace metals on lymphocyte growth and maturation , thymidine uptake ( TU ) , protein , ATP , Fe , Cu , Zn , ferritin , CD 3 , CD 4 , CD 8 antigens , surface transferrin receptors ( TFR ) and interleukin 2 receptors ( IL2R ) were assessed in normal and T cell leukemia human lymphocytes , cultured in media with varying Fe , Cu and Zn concentrations [ Me ] . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
To evaluate if Ag targeting to receptors expressed on APC could be an effective tool for promoting Ag uptake and presentation , we tested the capacity of activated T cells not expressing TfR to induce HBenvAg specific T cell responses when pulsed with a hybrid particle containing HBenvAg coupled to gp 120 of human immunodeficiency virus ( HIV ) , exploiting the ability of gp 120 to bind to CD 4 receptor . ^^^ The finding that the addition of anti CD 4 monoclonal antibody ( mAb ) before the pulsing of CD4+ / TfR T cells with the hybrid particle drastically blocked the specific T cell response , together with the finding that CD8+ / TfR T cells were unable to serve as APC even if pulsed with this molecule , demonstrated that CD 4 receptor was crucial for the HBenvAg internalization . ^^^ On the other hand , HBenvAg presentation by CD4+ / TfR+ T cells pulsed with the hybrid particle was inhibited only when both anti CD 4 and anti TfR were added before the pulsing . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
We measured the soluble ( s ) receptors CD 23 , CD 8 , CD 4 , interleukin 2 receptor ( IL 2R , CD 25 ) , and transferrin receptor ( TfR , CD 71 ) , in normal serum and in patients with chronic lymphocytic leukemia ( CLL ) and evaluated them in relation to clinical and biological parameters of the disease , as well as serum immunoglobulin E ( IgE ) . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
With 18 patients afflicted with recently diagnosed , non leukemic stages of B and T cell lymphoma cytofluorimetric analysis was performed with peripheral blood lymphocytes ( PBL ) after 72 h in culture with and without phytohemagglutinin , using antibodies against the differentiation antigens CD 3 , CD 8 , CD 4 , CD 16 , CD 19 , CDw 14 , and the activation antigens interleukin 2 receptor ( IL 2R , CD 25 ) , HLA DR ( DR ) , CD 56 and transferrin receptor ( TR ) . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
In parallel , cytofluorimetric analysis was done with PBL after 72 hours in culture with and without PHA , using antibodies against the differentiation antigens : CD 3 , CD 8 , CD 4 , CD 19 , and CDw 14 and the activation antigens : interleukin 2 ( IL 2 ) receptor ( IL 2R , CD 25 ) , human leukocyte antigen DR ( HLA DR ) , and transferrin receptor ( TR ) . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
There were no differences between TR and NTR in age at seroconversion ( 32 . 4 years each ) , in time from seroconversion to AIDS ( 67 versus 79 months ) , in mean CD 4 number ( 245 / microl ] versus 260 / microl ) ; nor in the proportion who developed AIDS ( 4 of 5 [ 80 % ] versus 24 of 34 [ 71 % ] ) . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Therefore , the absence of CD 4 ( + ) T cell response in the first week of infection might explain the non clearance of pathogen in TR mice . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Mouse CD 4 ( + ) Tr cells that express CD 25 possess remarkable suppressive activity in vitro and in autoimmune disease models in vivo . ^^^ Thus far , the existence of a similar subset of CD 25 ( + ) CD 4 ( + ) Tr cells in humans has not been reported . ^^^ Here we show that human CD 25 ( + ) CD 4 ( + ) Tr cells isolated from peripheral blood failed to proliferate and displayed reduced expression of CD 40 ligand ( CD40L ) , in response to T cell receptor mediated polyclonal activation , but strongly upregulated cytotoxic T lymphocyte associated antigen ( CTLA ) 4 . ^^^ Human CD 25 ( + ) CD 4 ( + ) Tr cells also did not proliferate in response to allogeneic antigen presenting cells , but they produced interleukin ( IL ) 10 , transforming growth factor ( TGF ) beta , low levels of interferon ( IFN ) gamma , and no IL 4 or IL 2 . ^^^ Importantly , CD 25 ( + ) CD 4 ( + ) Tr cells strongly inhibited the proliferative responses of both naive and memory CD 4 ( + ) T cells to alloantigens , but neither IL 10 , TGF beta , nor CTLA 4 seemed to be directly required for their suppressive effects . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
A large number of CD 4 ( + ) Tr cells producing interleukin ( IL ) 4 , IL 10 and TGF beta accumulated with functionally depressed CD 8 ( + ) cytotoxic T lymphocytes ( CTLs ) at tumour sites on day 20 after subcutaneous ( s . c . ) inoculation of B 16 tumour cells . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Collectively , these data demonstrate that CD 4 ( + ) T cells exposed to G CSF in vivo acquire the properties of T regulatory ( Tr ) cells once triggered in vitro through the T cell receptor , including a peculiar cytokine production profile ( IL 10 ( ++ ) TGF beta 1 ( + ) IL 2 ( low / ) IL 4 ( low / ) ) , an intrinsic low proliferative capacity , and a contact independent suppression of antigen driven proliferation . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Several types of Tr cells exist , including CD 4 ( + ) T cells which express CD 25 constitutively and suppress immune responses via direct cell to cell interactions , and type 1 T regulatory ( Tr 1 ) cells , which function via secretion of interleukin ( IL ) 10 and transforming growth factor ( TGF ) beta . ^^^ The relationship between CD 25 ( + ) CD 4 ( + ) T cells and Tr 1 cells remains unclear . ^^^ Here , we demonstrate at the clonal level that Tr 1 and CD 25 ( + ) CD 4 ( + ) T cells are two distinct subsets of regulatory cells with different cytokine production profiles . ^^^ Furthermore , CD 25 ( ) CD 4 ( + ) T cells can be rendered anergic by IL 10 and differentiated into Tr 1 cells in the absence of CD 25 ( + ) CD 4 ( + ) T cells . ^^^ Together these data indicate that naturally occurring human CD 25 ( + ) CD 4 ( + ) T cells are distinct from IL 10 producing Tr 1 cells . . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Downregulation or upregulation of CD 4 ( + ) CD 25 ( + ) Tr cells might be a way to achieve better protection from neurodegenerative conditions induced by self destruction or avoid autoimmune inflammatory disease development , respectively . . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Unlike human normal DCs , which caused the activation of allogeneic CD 4 ( + ) and CD 8 ( + ) T cells , human regulatory DCs not only induced their anergic state but also generated CD 4 ( + ) or CD 8 ( + ) regulatory T ( Tr ) cells from their respective naive subsets in vitro . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
We studied therapeutic regimens before Ti and after treatment reinitiation ( TR ) , clinical events related to Ti , CD 4 cells and viral loads before Ti and at months 3 , 6 , 9 , and 12 after TR . ^^^ Viral loads evolution after TR was better if Ti was related to treatment failure ( P < 0 . 05 ) , was prolonged ( P < 0 . 05 ) , and if CD 4 cells were high before Ti and at TR ( P < 0 . 05 ) . ^^^ Median CD 4 cells was of 296 / mm ( 3 ) at month 12 after TR vs 382 before Ti . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Because MHC class 2 ( / ) ( class 2 ( / ) ) mice generate efferent Tr cells following a . c . inoculation , we conclude that conventional CD 4 ( + ) T cells are not needed for the development of efferent CD 8 ( + ) T cells . ^^^ Furthermore , Ab depletion of CD 4 ( + ) cells in both wild type mice ( remove both conventional and CD 4 ( + ) NKT cells ) and class 2 ( / ) mice ( remove CD 4 ( + ) NKT cells ) abrogated the generation of Tr cells . ^^^ We conclude that CD 4 ( + ) NKT cells , but not the class 2 molecule or conventional CD 4 ( + ) T cells , are required for generation of efferent CD 8 ( + ) Tr cells following Ag introduction into the eye . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Ag specific CD 4 ( + ) T cell lines and clones generated from these mice had cytokine profiles characteristic of Th 2 or type 1 Tr cells , and these T cells suppressed IFN gamma production by Th 1 cells . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
In the present study , venous blood was obtained from MS patients concurrent with MRI examination of the brain , and expression of chemokine receptors CCR 1 , CCR 2 , CCR 5 , CXCR 3 and CXCR 4 by CD 4 T cells and monocytes , proportions of Tr cells , as well as expression of CD45RO , CD 95 , CTLA 4 , HLA DR and interleukin ( IL ) 10 by Tr cells and non Tr ( CD 25 ) CD 4 T cells was analyzed by flow cytometry . ^^^ Proportions of Tr cells amongst all CD 4 T cells , and expression of IL 10 by Tr cells were increased in the patients with high LL and Gd enhancing lesions . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
CD 4 ( + ) CD 25 ( high ) T regulatory ( Tr ) cells , representing high IL 2 receptor alpha chain expressing cells , have been shown to inhibit proliferation and cytokine secretion by CD 4 ( + ) T cells that are assumed to represent important effector cells in auto aggressive immunity . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Besides CD 4 ( + ) CD 25 ( + ) T cells , different populations of regulatory CD 4 ( + ) T cells secreting high amounts of IL 10 ( T regulatory type 1 ( Tr 1 ) ) or TGF beta ( Th 3 ) have also been described in in vivo models . ^^^ These results confirm in vitro studies showing that CD 4 ( + ) CD45RB ( low ) do not contain high IL 10 producing cells and suggest that CD 4 ( + ) CD45RB ( low ) Tr cells maintain tolerance in vivo , in part indirectly , through the differentiation of IL 10 secreting Tr 1 cells . . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
We compare the CDR 3 spectratype distributions of 24 different TR 5 beta ( TRBV ) segments in nave CD 4 T cells from discordant MS twins and from healthy identical twins . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Although a great deal has been learned as to how 4 1BB mediated signaling governs the immunity of conventional T cells , the functional role of 4 1BB in the context of CD 4 ( + ) CD 25 ( + ) regulatory T cell ( Tr ) activation is largely unknown . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Experimental animal models for autoimmunity have demonstrated the existence and crucial role of CD 4 ( + ) CD 25 ( + ) T regulatory ( Tr ) cells in suppressing autoreactive T cells and promoting peripheral tolerance . ^^^ Recent in vitro functional studies showed that Tr cells are enriched in the CD 25 ( high ) cell population among CD 4 ( + ) T cells , and that they totally inhibit proliferation and cytokine secretion by CD 4 ( + ) T cells . ^^^ As it was reported that the suppressive activity of CD 4 ( + ) CD 25 ( + ) Tr cells is mainly through cell surface contact pathway , we secondly analyzed the expression of the functionally important cell surface molecules of CD 4 ( + ) CD 25 ( high ) Tr cells . ^^^ Two or three colour flow cytometry was used to identify and quantify CD 4 ( + ) CD 25 ( + ) Tr cells and CD 4 ( + ) CD 25 ( high ) Tr cells among blood CD 4 ( + ) T cells in MS patients without treatment vs . patients treated with either interferon beta ( IFN beta ) or glatiramer acetate ( GA ) or IFN beta + GA in combination vs . healthy controls ( HC ) . ^^^ Expression of functionally important surface molecules CD45RO , CD 69 , CD 95 , HLA DR , and intracellular CTLA 4 and IL 10 production by CD 4 ( + ) CD 25 ( high ) Tr cells were investigated . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
In addition to the well established role of natural CD 4 ( + ) CD 25 ( + ) regulatory T ( Tr ) cells in the maintenance of tolerance to self antigens , there is accumulating evidence for distinct populations of Tr cells induced in the periphery after encounter with pathogens and foreign antigens . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Studies in experimental animal models of human autoimmune diseases have revealed that CD 4 ( + ) CD 25 ( + ) T regulatory ( Tr ) cells are of thymic origin and have potentials in preventing auto aggressive immunity . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
We developed a standardized and highly reproducible protocol to differentiate Tr cells by repetitive exposure of naive peripheral blood CD 4 ( + ) T cells to allogeneic iDCs . ^^^ In addition , Tr 1 cells induced by iDCs do not require the presence of CD 4 ( + ) CD 25 ( + ) Tr cells for their generation , nor do they express high constitutive levels of CD 25 or the transcription factor FoxP 3 . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
We show that transient depletion of regulatory T ( Tr ) cells recovers the primary CD 4 T cells response in MyD 88 deficient mice , demonstrating that a major mechanism of TLR mediated activation of T cell responses is the blocking of suppression by regulatory T cells . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Immunization of NOD mice with autoantigens such as glutamic acid decarboxylase ( GAD ) 221 235 peptide ( p 221 ) can induce Ag specific CD 4 ( + ) T regulatory ( Tr ) cells . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Regulatory T ( Tr ) cell activity is enriched within the naturally arising CD 4 ( + ) CD 25 ( + ) Tr subset that has been shown to prevent a number of inflammatory diseases . ^^^ CD 4 ( + ) CD 25 ( + ) Tr cells control intestinal inflammation induced by both innate and adaptive immune responses via IL 10 and TGF beta dependent mechanisms . ^^^ Recent results have shown that CD 4 ( + ) CD 25 ( + ) Tr cells can cure established colitis , suggesting their utility for the treatment of inflammatory bowel disease . . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Among the CD 4 ( + ) Tr cells , T regulatory type 1 cells ( Tr 1 ) have been shown to downmodulate immune responses through production of the immunosuppressive cytokines IL 10 and TGF beta . ^^^ Murine Tr 1 cells can be induced to differentiate in vitro by activating naive CD 4 ( + ) T cells in the presence of high doses of IL 10 . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Active suppression mediated by CD 4 ( + ) CD 25 ( + ) T regulatory ( Tr ) cells plays an important role in the down regulation of T cell responses to both foreign and self Ags . ^^^ In this report we show that human PF 4 stimulates proliferation of the naturally anergic human CD 4 ( + ) CD 25 ( + ) Tr cells while inhibiting proliferation of CD 4 ( + ) CD 25 ( ) T cells . ^^^ In coculture experiments we found that CD 4 ( + ) CD 25 ( + ) Tr cells exposed to PF 4 lose the ability to inhibit the proliferative response of CD 4 ( + ) CD 25 ( ) T cells . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Here , we provide new evidence that rapamycin selectively expands the murine naturally occurring CD 4 ( + ) CD 25 ( + ) FoxP 3 ( + ) Tr cells in vitro . ^^^ Based on this new mode of action , rapamycin can be used to expand CD 4 ( + ) CD 25 ( + ) FoxP 3 ( + ) Tr cells for ex vivo cellular therapy in T cell mediated diseases . . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
This study was designed to determine the role of CD 25 ( + ) CD 4 ( + ) regulatory T ( Tr ) cells in CTL maturation and effector functions using a murine CTL line and in vitro MLC . ^^^ In CD 4 ( + ) responder T cell depleted MLC supplemented with IL 2 , Tr cells suppressed mature CTL generation only when added within the first 2 days of culture . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Briefly , T regulatory ( Tr ) 1 cells as well as T helper ( Th ) 3 T cells express common T cell markers such as CD 4 and are characterized by secretion of IL 10 and TGF beta , which provides a means by which proliferation of conventional CD 4 ( + ) cells is blocked . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Immunohistological analysis of CD 4 , CD 25 , and CTLA 4 and the gene expression analysis of FOXP 3 , TGF beta 1 , and IL 10 on gingival biopsies revealed the presence of CD4+CD25+ Tr cells in all tissues . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
The difference and similarity between bovine activated CD 8 ( + ) T cells ( CD 8 ( + ) CD 26 ( + ) ) and well established human CD 4 ( + ) CD 25 ( + ) T regulatory ( Tr ) cells may help to reveal their unique immune regulatory system in the host infected with S . aureus . . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
To date , three main groups of Treg can de distinguished , mainly by their functional properties ( for review see Jonuleit and Schmitt 2003 ) Briefly , T regulatory ( Tr ) 1 cells as well as T helper ( Th ) 3 T cells express common T cell markers such as CD 4 and are characterized by secretion of IL 10 and TGF beta , which provides a means by which proliferation of conventional CD4+ cells is blocked . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
DCs differentiated in the presence of VIP impair allogeneic haplotype specific responses of donor CD 4 ( + ) cells in mice given transplants by inducing the generation of Tr cells in the graft . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Neither CD 4 cell count at TI , numbers of treatments , nor duration of treatment and infection before TI were independent predictive factors of early treatment resumption ( TR ) . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Adoptive transfer experiments revealed that DNA vaccination generated protective CD 4 ( + ) regulatory T cells ( Tr ) of either CD 25 ( + ) or CD 25 ( ) phenotype . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Mononuclear cells obtained by density gradient cytocentrifugation were stained with monoclonal fluorescein conjugated antibodies detecting CD 4 ( T helper ) , CD 8 ( T suppressor ) , CD 25 ( Interleukin 2 receptor ) , and the CD 71 ( Transferrin receptor ) antigens . ^^^ However , patients with moderate rejection showed a significant elevation of CD 4 positive cells and activated T cells ( CD 15 , CD 71 positive cells ) in the coronary sinus as compared with cells from the right atrium . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
The phenotype of the 1 , 25 DHCC induced cells shows light adherence , class I+ , increase in CD 4 and CD 14 and decrease in CD 71 . ^^^ The PMA induced cell is tightly adherent , class I+ , and strongly positive for CD 13 with a concomitant decrease in both CD 4 and CD 71 . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
On Day 2 we observed a reduced proportion of transferrin receptor ( CD 71 ) positive CD 4 OX44 cells , representing the cycling immature CD 4 CD8+ cells , and of CD71+OX44 cells , representing the cycling CD4+CD8+ cells , but not of CD71+CD4 CD 8 cells . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Useful methods for characterizing AIDS related non hodgkin ' s lymphomas include immunophenotypic studies using B cell differentiation and activation antigens ( HLA DR , CD 10 , CD 19 , CD 20 , CD 21 , CD 22 , CD 23 , CD 38 ) , evaluation of expression of surface immunoglobulins ( IgS ) , activation and proliferation ( CD 25 , CD 30 , CD 71 , Ki 67 ) , and identification of T cell markers ( CD 1 , CD 2 , CD 3 , CD 4 , CD 5 , CD 7 , CD 8 ) . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Several phenotypic markers ( CD 38 , CD 71 , and HLA DR ) that are susceptible to short term effects of ZDV ( but with changes that differ from CD 4 T cell changes ) are surrogate marker candidates for evaluation in anti HIV treatment . . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Osteoclast like cells were positive for CD 4 , CD 13 , CD 45 , CD 68 , CD 71 , and vimentin , but negative for lysozyme and HLA DR . ^^^ Mononuclear tumor cells were positive for CD 4 , CD11c , CD 13 , CD 14 , CD 45 , CD 68 , CD 71 , HLA DR , and vimentin , but negative for lysozyme . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
TNF induced expression of CD 4 and CD 71 , increased the intensity of HLA DR , CD 25 , CD11c and CD 13 expression and decreased both the intensity and frequency of sIg and cIg positivity . alpha IFN decreased CD 25 expression , the tartrate resistant acid phosphatase activity ( TRAP ) , reduced the TNF induced CD 4 and CD 71 expression and antagonized the TNF effect on the Ig expression . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Indirect immunofluorescence and monoclonal antibodies to leukocytic differentiated antigens CD 3 , CD 4 , CD 8 , CD 22 , CD 25 , CD 71 and HLA DR were used . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
CD 4 , CD 54 , CD 58 , and CD 71 markers become highly expressed on the cells after about 10 days in vitro while CD 56 is permanently lacking . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Surface markers on T lymphocytes ( CD 3 , CD 4 , CD 8 ) , B lymphocytes ( CD 19 ) and NK cells ( CD16 / 56 ) were normal in both groups ; however , in the acromegalic subjects , CD4+ and CD8+ cells showed a significant higher expression of transferrin receptors ( CD 71 ) , indicating enhanced T cell activity . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
These markers consist of T cell specific antigens ( CD 2 , CD 3 , CD 4 , and CD 8 ) , activated T cell antigens ( CD 25 and CD 71 ) , major histocompatibility complex ( MHC ) antigens ( class 1 and 2 ) , and adhesion molecules ( LFA 1 and ICAM 1 ) . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Flow cytometry was used as a quantification technique to measure lymphocyte subpopulations , CD 3 ( T cells ) , CD 19 ( B cells ) , CD 16 ( natural killer cells ) , CD 4 ( T helper / inducer cells ) , CD 8 ( T cytotoxic / suppressor cell ) , CD 25 ( interleukin 2 receptor ) , CD 69 ( activation inducer molecule marker ) , CD 71 ( transferrin receptor ) and CD 54 ( ICAM 1 ) ; CD4 / CD8 ratios were also estimated . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
The blasts were positive for CD1a , CD 4 , CD 16 , CD 35 , HLADR , HLADQ , CD11b , CD11c , CD 14 , CD 33 , CD 34 , CD11a , CD 71 , CD 19 , CD 25 , IL 2R beta and negative for CD 2 , CD 7 , CD 8 , CD 10 , CD 22 , CD 56 , CD 57 , surface or cytoplasmic CD 3 , TCR delta and TCR beta , HTLV 1p19 and P glycoprotein . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
We studied the lymphocyte activation markers CD 25 ( interleukin 2 receptor ) , CD 38 , CDw49b ( VLA 2 ) , CD 71 ( transferrin receptor ) , and HLA DR , as well as the surface antigens CD 3 , CD 4 , CD 7 , and CD 8 by two color flow cytometry in 23 chronic HD patients before and after a single dialysis session ; we also studied 30 normal controls . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
In contrast , TGF beta 1 did not influence the constitutive or activation induced expression of CD 4 , CD 8 , CD45RA , CD45RO , CD 25 , CD 71 , CD 54 , CD 58 , CD 59 , and B 7 . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Therefore , we analyzed the expression of CD 71 on fetal , neonatal , and adult thymocytes in correlation with cell size , cell cycle status , and expression of CD 3 , CD 4 , CD 8 , alpha beta TcR , and gamma delta TcR . ^^^ Phenotypic analysis showed that only the large , immature CD 4 8 3 , CD 4 8+3 , and CD4+8+3 cells in fetal , neonatal , and adult thymus expressed CD 71 . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Other non B cell lineage markers such as myeloid ( CD 13 , CD 14 , CD 15 , CD 33 , CD 41 , and glycophorin A ) , T cell ( CD 2 and CD 4 ) , and natural killer associated ( CD 56 ) antigens , as well as CD 23 , CD 24 , CD 25 , CD 37 , CD 39 , CDw 40 , CD45R , CD 71 , and certain unclustered antigens ( R 1 3 , PCA 1 , PCA 2 , PC 1 , 62B1 , 8A , 8F6 , and MM 4 ) , have been noted in myelomatous PCs . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
The release is mediated by a Zn ( 2+ ) dependent , membrane bound metalloprotease that does not cleave HLA DR , CD 4 , and CD 71 surface receptors and can be activated by phorbol myristate acetate . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
These were compared with the proportion of CD 4 CD45RO , IL 2R ( CD 25 ) , and transferrin receptor ( CD 71 ) positive peripheral blood lymphocytes . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
IFN gamma was specifically produced from CD16+ natural killer ( NK ) cells under stimulation by S . aureus Cowan 1 or Wood because these strains ( 1 ) induced IFN gamma production exclusively from CD 3 , CD 4 CD 8 , and CD16+ cells and ( 2 ) induced CD 69 and interleukin 2 ( IL 2 ) receptor alpha expression on CD16+ cells without simultaneously augmenting CD 71 or IL 2 receptor alpha on T cells . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
We have studied the expression of several leukocyte surface markers ( CD 2 , CD 3 , CD 4 , CD 8 , CD 7 , CD 26 , CD 25 , CD 14 , CD 18 , kCD 20 , CD 43 , CD 45 , CD45R , CD 71 and HLA class 1 and 2 ) and we find no differences in their expression between PPL . 1 and its parental Jurkat cell line . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
The surface expression of CD 13 ( aminopeptidase N ) , CD 4 , CD 35 ( C3b receptor ) , CD 64 ( Fc gamma RI ) , CD 71 ( transferrin receptor ) and HLA Class 2 was not affected by probucol . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Two types of large complexes containing protein tyrosine kinases were observed : the first one contained CD 3 and the transmembrane proteins CD 2 , CD 4 , CD 5 , CD 6 , CD 7 , CD 8 , CD11a , CD 38 , CD 43 , CD 45 , CD 71 , CD 98 and CD 99 and the other contained mainly the GPI anchored proteins CD 48 , CD 55 , CD 59 and CDw 108 as well as a fraction of CD 4 and CD 8 . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Duallabelling studies were conducted to compare the reactivity patterns of LR 1 and the HSA reactive monoclonal antibody J11d and indicated that both antibodies recognized splenocytes bearing B cell ( IgM ) or erythroid ( TER 119 , CD 71 ) but not T cell ( CD 4 , CD 8 ) markers . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Both normal and CAPD PM phi expressed CD 4 , CD 69 , and CD 71 very weakly and lacked CD 25 . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Because fluvastatin did not affect the expression of other cell surface markers , CD 4 and CD 71 , the inhibitory effects of fluvastatin on adhesion molecule expression could not be attributed to the non specific suppression of the cell . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
The percentage of cells positive for CD 19 , CD 25 , CD 38 , and CD 71 was significantly lower during convalescence compared with pre IVIG ( medians : CD 19 , 18 % vs 26 % , P = 0 . 0004 ; CD 25 , 6 % vs 9 % for CD 3 ( + ) cells , P = 0 . 0074 ; CD 38 , 78 % vs 89 % for CD 8 ( + ) cells , P = 0 . 0015 ; CD 71 , 1 % vs 6 % for CD 4 ( + ) cells , P = 0 . 0024 ) . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
We have shown that treatment of the CD 4 T lymphocytes with HIV 1 gp 120 before anti CD 3 stimulation impedes cell cycle progression as measured by reduced CD 71 expression and inhibition of [ 3H ] thymidine incorporation . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Peripheral blood mononuclear cells stimulated by phytohemagglutinin ( PHA ) were simultaneously phenotyped for PpIX , CD 71 and the T cell markers CD 3 and CD 4 or CD 8 . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
The vast majority of DPP 4 positive T ALL cases expressed CD 4 , CD 8 , CD 7 , CD 5 , CD 2 along with CD 71 and CD 38 antigens , but the cells were surface membrane CD 3 antigen negative . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Activated T cells treated with sub lethal levels of BPD MA and light exhibited lower CD 25 levels , a temporary block in cell cycle transition , but unaltered expression of MHC Class 1 , CD 3 , CD 4 , CD 8 , CD 45 , CD 54 , CD 71 , CD 122 ( IL 2R beta chain ) or TCR beta chain antigens 24 h afterward . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
RESULTS : Laparoscopy was found to cause significantly less reduction in the number of cells expressing T lymphocyte phenotypic surface markers ( CD 2 , CD 3 , CD 8 , CD 4 : CD 8 ratio ) , activation markers ( CD 71 and HLA DR ) , and NK cell subsets ( CD11b , CD 16 , CD 56 and CD 57 ) , when compared with the minilaparotomy technique . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
A small fraction of rat 13 day old fetal liver ( r13FL ) cells , which weakly expressed the leukocyte common antigen CD 45 , constituted a homogeneous Thy 1 ( hi ) , CD 71 ( ) , CD 44 ( + ) , MHC class I+ , CD 43 ( + ) cell subpopulation negative for CD45RC , CD 3 , TCRalphabeta , TCRgammadelta , CD 2 , CD 5 , CD 4 , CD 8 , CD 25 , CD 28 , NKR P1a and sIg . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
We investigated the effects of acute injection of morphine into the rat mesencephalon periaqueductal gray ( PAG ) , on splenic natural killer ( NK ) cell and lymphocyte functions , interleukin 2 ( IL 2 ) production , expression of T cell ( CD 3 ) , T helper cell ( CD 4 ) , T suppressor cell ( CD 8 ) , and NK cell ( NKR P 1 ) surface markers , and expression of IL 2 ( CD 25 ) and transferrin ( CD 71 ) receptors . ^^^ Furthermore , morphine did not alter the expression of CD 3 , CD 4 , CD 8 , and NKR P 1 surface markers , but significantly ( p < 0 . 001 ) downregulated the expression of CD 25 and CD 71 receptors following in vitro activation . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
We evaluated FN ( fibronectin ) , CD 4 and CD 71 expression in uninfected and acutely or chronically infected cells , both untreated and treated with Nef . ^^^ Our studies showed a significant up regulation of FN especially in uninfected cells , with a dose of 2 . 5 microg ml ( 1 ) ; in contrast , a significant down modulation of CD 4 and CD 71 both in uninfected and in acutely or chronically infected cells , was detected . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Her bone marrow was normocellular with an excess of blasts ( 89 . 1 % , peroxidase stain ( ) , PAS stain ( ) ) that displayed a positive immunophenotype for CD 2 , CD 4 , CD 5 , CD 7 , CD 34 , CD 38 , and CD 71 . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
K 2 MDS cells are positive for the expression of CD 4 , CD 5 , CD 13 , CD 25 , CD 71 , CD 95 , HLA DR and cytoplasmic CD 3 . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Moreover , leptin dose dependently enhanced the expression of the late activation markers CD 25 and CD 71 in both CD 4 ( + ) and CD 8 ( + ) T lymphocytes after 48 h stimulation with PHA or Con A . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Expression of CD 3 , CD 4 , CD 8 , CD 25 , CD 38 , CD 56 , CD 71 , CD 122 , and HLA DR antigens , serum level of the soluble IL 2R alpha , and NK activity against K 562 cell line were evaluated in 11 patients representative for both types of immunotherapy . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Most ( > 75 % ) agreed that at least one more B ( CD 20 , CD 22 , CD79a , IgM ) , T ( CD1a , CD 2 , CD 4 , CD 5 , CD 8 ) , myeloid ( CD11b , CD 15 , CD 64 , CD 117 , myeloperoxidase ) , erythroid ( CD 36 , CD 71 , glycophorin A ) , and megakaryocytic ( CD 41 , CD 61 ) reagents should be included in the essential panel . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
The expression of CD 3 , CD 4 , CD 7 , CD 8 , CD1a , CD 34 , CD 68 , CD 71 , Ki 67 , bcl 2 and p 53 was studied immunohistochemically . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
MAb to antigens CD 25 , CD 8 , CDw 50 , CD 5 , CD 4 , CD 7 , CD 3 , CD 71 , CD 34 , CD 45 , CD 38 , CD11b , CD 16 , and CD 95 have gained worldwide recognition at International HDLA Workshop Conferences . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Frequencies of activated CD 8 lymphocytes were higher in HIV infected study subjects than in seronegative controls , and they frequently coexpressed CD 4 ( mean frequencies on CD 69 ( + ) , CD 71 ( + ) , and HLA class 2 ( + ) cells of 23 , 37 , and 8 % , respectively , compared with 1 to 2 % for nonactivated CD 8 lymphocytes ) . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Tumor process involved enhanced expression of the activation markers CD 25 , CD 71 and HLA DR as well as differentiation antigens CD 4 , CD 8 , CD 22 and CD 16 . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
An arthritogenic subpopulation of CD 4 ( + ) T cells , enriched by either negative or positive selection , expressed the activation markers CD 25 ( IL 2 receptor alpha ) , CD 71 ( transferrin receptor ) , CD 134 ( OX 40 antigen ) and MHC class 2 . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
The studied antigens were the following : CD 3 , CD 4 , CD 8 , CD 14 , CD 19 , CD 25 , CD 38 , CD 45 , CD45RO , CD 54 , CD 56 , CD62L , CD 71 and HLA DR . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
The composition of the oral cavity and intestine microflora as well as the total IgE level , peripheral blood eosinophils and lymphocytes expressing receptors CD 3 , CD 4 , CD 8 , CD 22 , CD11b , CD 71 , HLA DR were detected . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
The immunoprofile of L 82 corresponds to that seen typically in primary ALCL cells , with positivity for CD 30 , EMA , CD 3 , CD 4 , CD 25 , CD 71 , TIA 1 , and granzyme B ; the cells were negative for EBV related antigens . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
In particular , IFN gamma shows a significant effect on cell replication and on reduction of CD 4 and CD 71 surface expression . . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Lymphocyte proliferation , expression of T cell surface activation antigens ( CD 25 , CD 71 , CD11a , CD 95 , CD 154 ) , production of intracellular cytokines ( IL 2 , INFgamma , TNFalpha ) , and lymphocyte subsets ( CD 4 , CD 8 , CD 16 , CD 20 ) were assessed by flow cytometry . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Routine analysis and lymphocyte phenotyping by an immunoperoxidase technique with the monoclonal antibodies CD 3 , CD 20 , CD 4 , CD 8 , CD 57 , CD 1 , CD 25 , CD 71 and HLA DR were performed . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
The number of CD 3 ( + ) , CD 4 ( + ) , CD 8 ( + ) , CD 25 ( + ) , CD 71 ( + ) and CD 26 ( + ) lymphocytes was also evaluated . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
CONCLUSIONS : CD 4 positive lymphocytes in BALF were increased in MCTD compared to PM DM patients , while CD 71 positive AM were decreased in MCTD compared to SSc patients . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
We studied a group of 42 patients with SSc and a group of 28 matched normal controls by flow cytometry using the lymphocyte cell surface markers CD 2 , CD 3 , CD 4 , CD 8 , CD 19 , CD 25 , CD45RA , CD 56 , CD 71 , HLA DR , TCR alpha / beta , and TCR gamma / delta . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
After cell count and differentiation of second morning urine samples with New Sternheimer and Papanicolaou stains , immunocytochemistry was performed using antibodies against the following antigens : CD 2 , CD 4 , CD 8 , CD 25 , CD 71 ( transferrin receptor ) , HLA DR and cytokeratin ( Lu 5 ) . ^^^ Immunocytochemical analysis showed a significant increase in CD 2 , CD 4 , CD 8 , CD 25 , CD 71 , and HLA DR positive epithelial cells and in the ratio HLA DR / cytokeratin positive epithelial cells in rejection . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Circulating cytokines , C reactive protein ( CRP ) , cortisol , and cell surface receptor expression of immune cells ( cluster of differentiation [ CD ] 14 , human leukocyte antigen DR , CD 86 , CD 71 , CD 3 , CD 4 , CD 8 , CD 16 , and CD 19 ) were measured perioperatively to characterize immunological functions . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
RESULTS : HR 8348 cell line did not express CD 2 , CD 3 , CD 4 , CD 5 , CD 7 , CD 8 , TCR , CD 10 , CD11b , CD 14 , CD 16 , CD 19 , CD 22 , CD 25 , CD 28 , SmIg , CD 33 , CD 35 , CD 36 , CD41a , CD 45 , CD45RA , CD45RO , CD 56 , CD 61 , CD 64 , CD66b , CD 69 , CD 71 , CD 117 , CD 122 and P glycoprotein but expressed CD 13 , CD 15 , CD 20 , CD 38 , CD 95 and HLA DR . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
We describe here that primary human adenotonsillar memory phenotype CD45R0 ( + ) CD 4 ( + ) T cells , but not adenotonsillar naive phenotype CD45RA ( + ) CD 4 ( + ) T cells , or peripheral blood naive or memory CD 4 ( + ) T cells , express high levels of activation associated antigens CD 38 , CD 69 , CD 71 , and HLA DR . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Indirect immunofluorescence was used to determine the count of CD 3 , CD 4 , CD 80 , CD 38 , CD 25 , CD 71 , and CD 95 lymphocytes . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
TPA induced the following antigens in decreasing order of the change : CD11c , CD 9 , CD11b , CD 54 , CD 38 , CD45RO and CD66c , with repression of CD 4 , CD 117 , CD 95 , CD 71 and CD 64 . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
Subclasses of T , B and NK lymphocytes were tested for in the blood samples : CD 3 , CD 4 , CD 5 , CD 7 , CD 8 , CD11B , CD 16 , CD 20 , CD 25 , CD 38 , CD45RA , CD 50 , CD 71 and CD 95 . ^^^
Interacting proteins: P02786 and P01730 Pubmed SVM Score :0.0
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