Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.57932451
We show that CD 4 ( + ) thymocytes with identical specificity for the S 1 self peptide can undergo overt deletion versus abundant selection to become CD 4 ( + ) CD 25 ( + ) regulatory T cells in response to variations in expression of the S 1 self peptide in different lineages of HA transgenic mice . 0.57932451^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
T cells expressing Tac antigen could be CD 4 CD 8 , CD4+ CD 8 , CD 4 CD8+ , or CD4+ CD8+ . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The IL2R alpha chain expression was decreased on CD 4 and CD 8 T cells from HIV+ persons compared to controls . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The proliferating CD 4 ( + ) T cells maintained IL2R expression . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
YOS M expressed myeloid associated antigens ( CD 14 , CD 33 ) as well as CD 4 , CD 25 and CD 34 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The following monoclonal antibodies were used : CD 4 , CD 8 , CD 57 , CD 25 , HLA DR . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
No cytokines induced CD 3 , CD 4 , CD 23 , CD 25 or CD 45 RA antigen expression in organ culture . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Flow cytometry of the abnormal lymphocytes showed that they expressed CD 4 , and CD 25 , but not CD 8 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Antibodies to HLA DR , CD 2 , CD 4 , CD 8 , and CD 25 were also demonstrated but could not be quantified . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
ATL cells show usually positive for CD 2 , CD 3 , CD 4 , CD 25 and HLA DR , but negative for CD 8 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Their surface phenotypes ( CD 3 , CD 4 , CD 8 , CD 16 , CD 25 , Leu 7 , HLA DR ) were measured by FACS . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The tumor cells represented T cell markers , usually CD 4 , but CD 25 was negative . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
PBMC were tested with a panel of monoclonal antibodies ( MoAbs ) including anti CD 3 , CD 4 , CD 8 , CD 25 reagents . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
No significant differences were found between the groups regarding cells positive for CD 3 , CD 4 , CD 8 , CD 20 or CD 25 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The expressions of LFA 1 , VLA 4 , CD 4 , CD 8 and CD 25 ( IL 2R ) molecules were decreased in PUVA treated Con A blasts . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
No significant changes in CD 3 , CD 4 , CD 8 , CD 16 , and CD 25 were observed . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Antibodies against CD 4 , CD 25 or HLA DR produce more limited immunomodulatory effects . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Surface markers were positive for CD 4 , CD 25 and HLA DR , and negative for CD 3 , CD 8 , CD 16 , CD 56 and CD 57 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 3 , CD 4 , CD 8 , CD11b , CD 16 , CD 25 , CD 57 , Leu 8 HLA DR were compared . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Flow cytometry showed that they were T cell lines expressing CD 2 , CD 3 , CD 4 , CD 8 and CD 25 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The expression of CD 4 , CD 25 and CD45RO were detected only in low density . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The surface phenotype of lymphocytes was positive for CD 2 , CD 4 , CD 25 , CD 29 suggesting helper inducer T cell . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 and CD 8 subsets were normal but 40 % of CD 3 cells were Ia positive although not CD 25 positive ( IL 2 receptor ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Both normal and CAPD PM phi expressed CD 4 , CD 69 , and CD 71 very weakly and lacked CD 25 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Eosinophils in blood and mammary glands were negative for MHC class 2 , CD 25 and CD 4 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Cells were labelled with CD 3 , CD 4 , CD 25 , CD45RO or alpha 4 beta 7 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 3 , CD 4 , and CD 25 counts were similar in the two groups . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The percentages of CD 4 and CD 25 positive cells in the infiltrating cells in the CNS were reduced by this treatment . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
After triple staining , cells were analyzed by flow cytometry for CD 4 , CD 8 , and CD 25 antigens . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
They did not express CD 3 , CD 4 , CD 5 , CD 10 , CD11b , CD 13 , CD 25 , CD 56 , CD 14 , CD 33 , or CD 64 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
A panel of monoclonal antibodies was used : CD 5 , CD 4 , CD 8 , CD 25 , and CD45RA . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Percentages of lymphocytes expressing CD 3 , CD 4 , CD 8 , CD 56 , CD 25 , CD45RA , CD45RO , and HLA DR were determined . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Coligation of CD 3 and CD40L increased expression of CD 69 , CD 25 , and CD 54 on CD 4 ( + ) T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Expression of CD 4 , CD 8 , CD 25 , CD 38 , L selectin , CD 44 , alphaEbeta 7 and alpha4beta7 were studied . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Activated CD 4 lymphocytes bearing CD 25 and human leukocyte antigen ( HLA ) DR in BALF were higher than in PB . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
A two to fourfold decline in CD 4 ( + ) T lymphocytes expressing CD 25 and CD 69 was seen later in SIV infection . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) T cells from the spleen retained anergic suppressive properties that were reversed by IL 2 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) , CD 25 ( + ) T cells as regulators of alloimmune responses . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
McLCL stimulated both CD 4 ( + ) and CD 8 ( + ) T cells as measured by HLA DR and CD 25 expression using FACS analysis . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
This was associated with a significantly increased proportion of activated CD 4 ( + ) lymphocytes ( CD 25 ( + ) ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Certified professionals : CD 4 ( + ) CD 25 ( + ) suppressor T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In contrast , CD 25 ( Il 2R ) and CD 27 were expressed at lower levels by the AV2S3 positive CD 4 ( + ) lung T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Activation of CD 4 ( lo ) cells is suggested by their expression of CD 25 and increased amounts of HLA DR . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
PBMC were incubated with mAb against porcine CD 3 , CD 4 , CD 8 , CD 25 , CD 45 , IgM , SWC 3 , and SLA Class 2 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 25 ( + ) CD 4 ( + ) regulatory T cell function also appears to be related to TFG beta . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
ATL cells were CD 2 , CD 3 , CD 4 , CD 25 positive and CD 8 negative . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Immunohistology was performed using monoclonal mouse anti rat antibodies against CD 4 , CD 8 , CD 25 , CD 45 and CD 54 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 and CD 8 T cells expressed similar CD 25 profiles although CD 28 expression was unique in each subset . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In normal mice a subpopulation of CD 4 T cells constitutively expresses the IL 2 receptor alpha chain ( CD 25 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Moreover , the epithelial layer in transgenic mice was significantly enriched for CD 4 ( + ) CD 25 ( + ) T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
LT 1 and LT 3 were CD 4 ( + ) CD 25 ( ) and produced IFN gamma in response to col ( 5 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Regulatory CD 25 ( + ) CD 4 ( + ) T cells are considered as important players in T cell homeostasis and self tolerance . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 CD 25 regulatory T cells are not functionally impaired in adult patients with IgE mediated cow ' s milk allergy . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The activated CD 4 ( + ) donor T cells that expressed CD 25 were purified by cell sorting . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Other authors have correlated the migration of DCs to the thymus with the generation of CD 4 ( + ) CD 25 ( + ) THYr . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Reporter gene expression was detected in CD 4 ( + ) T cells bearing the activation markers CD 25 , CD 44 , and CD 69 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The average number of CD 4 ( + ) and CD 25 ( + ) T lymphocytes also decreased ( both P < . 05 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Both CD 25 and MHC class 2 expressing CD 4 ( + ) T cells were detected from day 7 onwards , peaking around day 13 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
MW exposure restored the CD 25 surface activation marker expression in CD 4 ( + ) T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
This could be a role for regulatory T cells , possibly those expressing both CD 4 and CD 25 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
These CD40L ( + ) T cells were CD 4 ( + ) , CD 69 ( + ) , but negative for CD 8 , CD 25 , CD 28 , and ICOS . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The MET 1 cells are activated T cells that express CD 2 , CD 3 , CD 4 , CD 25 , CD 122 , and CD 52 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) regulatory T ( T ( R ) ) cells are essential negative regulators of multiple immune functions . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Here , we show that CD 4 ( + ) CD 25 ( + ) human T ( R ) cells suppress virus specific T cell responses . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We found no increase in CD 4 ( + ) CD 25 ( + ) regulatory T cells in NOD TdT ( / ) mice . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) T regulatory cells infiltrating the tumors were markedly reduced after DC AdCCL 21 therapy . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Atypical large lymphocytes were positive for CD 30 , CD 4 and CD 25 , and negative for CD 3 and CD79a . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) regulatory T cells are crucial to the maintenance of tolerance in normal individuals . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
It is well established that CD 4 ( + ) CD 25 ( + ) regulatory T cells ( Tregs ) inhibit autoimmune pathology . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Cell activation was assessed by immunophenotyping using antibodies directed toward CD 4 , CD 25 , or CD 69 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) T cells can be used to interfere with spontaneous autoimmune diseases such as type 1 diabetes . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Naturally arising CD 25 ( + ) CD 4 ( + ) regulatory T cells actively maintain immunological self tolerance . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) ) were induced . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 25 ( IL 2 receptor alpha chain ) marks a population of CD 4 positive T cells with a suppressor phenotype . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Transfer activity was lost when cells were depleted of CD 4 ( + ) or CD 25 ( + ) subpopulations . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Analysis of FOXP 3 protein expression in human CD 4 ( + ) CD 25 ( + ) regulatory T cells at the single cell level . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) T cells and cytokines IL 10 and TGF beta 1 did not increase after challenge . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The phenotype of CD 3 , CD 4 , CD 8 , CD3CD56 and CD 25 was identified by flow cytometric analysis every two days . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Peripheral blood leukocytes were scanned for donor chimerism and CD 3 , CD 4 , CD 8 and CD 25 expression . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) regulatory T cells ( T ( reg ) cells ) play important roles in intestinal homeostasis . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Anti CD 4 , CD 5 , micro chain , CD 25 and RLA 2 monoclonal antibodies were used . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 + CD 25 + regulatory T cells and their importance to human illnesses ] . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Naturally occurring CD 4 ( + ) CD 25 ( + ) Tregs are important in the prevention of autoimmune diseases . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
This tolerance is mediated by CD 4 ( + ) CD 25 ( + ) T regulatory ( T ( reg ) ) cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The CD 25 and CD25+ CD 4 T lymphocyte compartments are tightly regulated . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Human CD 4 ( + ) CD 25 ( + ) regulatory T cells ( Treg ) play an essential role in the prevention of autoimmune diseases . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
TLR 4 signaling has been implicated in the regulation of CD 4 ( + ) CD 25 ( + ) Treg functions recently . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) T regulatory ( Treg ) cells are suppressor cells that can enhance alloengraftment . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Flow cytometric analyses of the lymphocytes revealed high expression of CD 4 and CD 25 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 T cells were purified and sorted into Treg ( CD 25 ( high ) ) and Treg depleted populations . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
However , although they do not express the T cell markers Thy 1 , CD 2 , CD 3 , CD 4 and CD 5 , 20 % 30 % of dendritic cells are positive for the interleukin 2 receptor alpha chain ( CD 25 ) , and about 30 % express intermediate levels of CD 8 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Development of immature thymocytes : initiation of CD 3 , CD 4 , and CD 8 acquisition parallels down regulation of the interleukin 2 receptor alpha chain . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Northern blot analysis of RNA prepared from the tumors revealed expression of human interleukin 2 , interleukin 2 receptor alpha chain , HB 24 , and CD 4 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In recent years , a population of CD 4 ( + ) T cells that constitutively express the interleukin 2 receptor alpha chain , CD 25 , has been shown to play a pivotal role in the maintenance of self tolerance in rodent models . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In addition to these passive mechanisms , evidence has accumulated for the active suppression of autoreactivity by a population of regulatory or suppressor T cells that co express CD 4 and CD 25 ( the interleukin 2 receptor alpha chain ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Factor 8 concentrate causes profound inhibition of IL 2 receptor expression ( CD 25 ) ; with an 89 % reduction in CD 25 positive CD 4 cells and a 50 % reduction in CD 25 antigen molecules per cell . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
TNF alpha up regulated the IL 2 receptor ( IL 2R ) alpha chain ( Tac antigen ) on the surface of CD3+ CD8+ CD 4 TAL , enhanced the proliferation of autologous tumor specific CTL , and potentiated their lytic function in long term cultures . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
On the other hand , the altered distribution of regulatory populations mostly due to an absolute decrease of the CD 4 subpopulation , associated to the hyperproduction of IL 2 and deficient expression of the Tac antigen in most of our patients , suggest the existence of functional alterations . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
These included : truncated proteins , lacking transmembrane and or cytosolic domains ; chimeric proteins , in which extracellular , transmembrane or cytosolic domains were replaced with similar domains derived from either the Tac antigen or CD 4 ; and point mutagenized TCR chains . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Taken together , TNC might be involved in the differentiation and / or expansion of murine fetal thymocytes by inducing IL2R beta chain , which forms the functional IL2R together with IL2R alpha chain and CD 4 , one of the T cell accessory molecules , on the cell surface through direct cell cell interaction . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
T cell antigen expression following PHA stimulation was assessed for helper ( CD 4 ) and suppressor ( CD 8 ) subpopulations : the early T cell activation antigens interleukin 2 receptor ( IL2R ) and transferrin receptor ( TFR ) , and the late antigen ( HLA DR ) using flow cytometry . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
After 7 days of culture with live virus , IL 2 receptors ( IL2R ) were found on CD 4 and CD 8 cells while cultures stimulated with fixed or extracted antigen had IL2R only on CD 4 cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Preoperatively , numbers of total lymphocytes and subclasses CD 3 , CD 4 , and CD 8 , as well as the interleukin 2 receptors ( IL2R ) , were the same as controls ( cells / microliters ) : lymphocytes , 1 , 940 ; CD 3 , 1 , 413 + / 410 ; CD 4 , 894 + / 318 ; CD 8 , 490 + / 185 ; IL2R , 96 . ^^^ From implant to postoperative day 5 , levels were below control values ( p less than 0 . 001 ) , reaching a nadir on postoperative day 2 ( lymphocytes , 896 + / 599 ; CD 3 , 489 + / 267 ; CD 4 , 309 + / 207 ; CD 8 , 183 + / 107 ; IL2R , 43 + / 47 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Cell sorting and cytotoxic depletion procedures were used to subdivide the population of CD 4 CD 8 ( `` double negative ' ' ) thymocytes from adult CBA mice on the basis of expression of Ly 1 , HSA ( the `` heat stable antigen ' ' M1 / 69 or B2A2 ) , Pgp 1 glycoprotein , Thy 1 , MEL 14 and the PC 61 antigenic determinant on the IL 2 receptor ( IL2R ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Based on reports that interleukin 2 receptor ( IL2R ) expression correlates well with proliferative responses in HIV infection , we used dual color cytofluorometry to measure IL2R expression by CD 4 and CD 8 cells following PHA activation of MC from HIV seropositive blood donors . ^^^ When compared to the seronegative control and sero + NML groups , the sero + LOW group exhibited significant reductions in the percentage of MC expressing IL2R , the proportion of CD 4 cells expressing IL2R , and the proportion of CD 8 cells expressing IL2R . ^^^ These findings show that reduced PHA induced proliferative responses by MC from HIV infected persons are associated with decreased IL2R expression by both CD 4 and CD 8 lymphocyte subsets . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Further , dual color analysis revealed that the proportion of CD 4 cells expressing IL2R was maximal on day 5 , whereas the proportion of CD 8 cells expressing IL2R continued to increase until day 9 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
To evaluate the modulatory effects of trace metals on lymphocyte growth and maturation , thymidine uptake ( TU ) , protein , ATP , Fe , Cu , Zn , ferritin , CD 3 , CD 4 , CD 8 antigens , surface transferrin receptors ( TFR ) and interleukin 2 receptors ( IL2R ) were assessed in normal and T cell leukemia human lymphocytes , cultured in media with varying Fe , Cu and Zn concentrations [ Me ] . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
At the cell surface marker level , MMTVD CD 4 thymocytes show a delay in surface expression of CD 3 , murine CD 4 and murine CD 8 , along with persistent expression of IL2R alpha compared with foetal non transgenic littermates . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Additionally , the expression of receptors for CD 14 and MHC class 2 on leucocytes , and the expression of receptors for CD 4 , CD 8 , WC 1 , IL2R and B cells on lymphocytes was measured in mammary secretions by flow cytometric analyses . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The percentage of different peripheral blood lymphocyte subsets ( CD 3 , CD 4 , CD 8 , CD 16 , CD 25 ) and the percentage of IL2R positive tumor cells on histologic sections were also measured . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The objective of this investigation was to study ( 1 ) the numbers of leukocytes , ( 2 ) the proportions of lymphocytes expressing CD 4 , CD 8 , WC 1 , B or IL2R and ( 3 ) neutrophil phagocytosis and oxidative burst activity in blood around parturition in three groups of dairy cows fed different levels of a total mixed ration during the last eight weeks before calving . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We used immunohistochemistry on cryopreserved human conjunctiva with monoclonal antibodies to T lymphocytes ( CD 3 , CD 4 and CD 8 ) , B lymphocytes ( CD 19 ) , macrophages ( CD 14 ) , natural killer cells ( NK , CD 57 ) , intercellular adhesion molecule 1 ( ICAM 1 ) , E selectin , vascular cell adhesion molecule 1 ( VCAM 1 ) , lymphocyte function associated antigen 1 ( LFA 1 ) , very late antigen 4 ( VLA 4 ) , interleukin 2 receptor ( IL2r , CD 25 ) and HLA DR . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
When fused to CD 4 or IL2RA ( interleukin 2 receptor alpha subunit ) proteins , which are normally distributed ubiquitously , the M 3 M4 intracellular loop from the alpha 7 subunit promoted dendritic expression , whereas the homologous M 3 M4 loop from the alpha 4 subunit led to surface axonal expression . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Mononuclear cells obtained by density gradient cytocentrifugation were stained with monoclonal fluorescein conjugated antibodies detecting CD 4 ( T helper ) , CD 8 ( T suppressor ) , CD 25 ( Interleukin 2 receptor ) , and the CD 71 ( Transferrin receptor ) antigens . ^^^ Mild rejection led to a statistically insignificant increase of CD 4 CD 25 and CD 7 antigen bearing cells in the coronary sinus blood , whereas the CD 8 positive cells remained stable as compared to mononuclear cells from the right atrium . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Flow cytometry analysis indicated that treatment with the anti CD 3 mAb increased the percentage of CD 3 positive cells , CD 4 positive cells and especially CD 25 positive cells , but decreased th percentage of CD 56 positive cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Surface phenotypic studies on nasal and cutaneous lesions demonstrated a population of T cells expressing CD 2 , CD 4 , CD 25 , CD 30 , and histocompatibility antigen DR ( HLA DR ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The number , distribution , and phenotype of mononuclear cells infiltrating the allograft lung transplant were determined immunohistochemically with monoclonal antibodies directed against cellular antigens ( CD 3 , CD 4 , CD 8 , CD 22 , CD 25 , CD 16 , CD 56 , CD 68 , HLA DR ) on frozen sections of transbronchial biopsies . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Leukemic cells in the peripheral blood and bone marrow were monoblasts positive for alpha naphthol butyrate esterase ( alpha NBE ) staining , CD11c and CD 36 antigens , whereas tumor cells in the pleural effusion were ATL cells positive for CD 2 , CD 4 , CD 25 , CD 29 and CD45RA antigens . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
TLC from both panels showed similar levels of expression of TCR alpha / beta , CD 4 , CD 2 , CD 25 , and CD 29 and recognized nickel in association with class 2 HLA molecules with restriction determinants in HLA DR , HLA DP , and HLA DQ . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We found that CD 25 expression was enhanced among PB CD 4 T cells of RA patients as compared with CD 4 cells of patients with reactive arthritis , degenerative joint disease or of healthy controls . ^^^ Within the CD 4 T lymphocytes subset we found that the CD45RO ( naive ) cells selectively in RA displayed higher levels of CD 25 protein and of interferon gamma mRNA expression when compared with the respective subset of all other investigated groups . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
On hematological examination , leukocyte count was 23 , 500 / microliters , of which 36 % abnormal lymphocytes expressing CD 2 , CD 3 , CD 4 and CD 25 as same as the lymph node cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We have previously described the effects of individual CD 4 ( BWH 4 ) , CD 25 ( IL 2R , ART 18 ) mAbs , and CsA therapeutic regimens upon cardiac allograft survival in sensitized hosts . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
A biopsy specimen of a skin lesion showed diffuse large cell lymphoma ; the lymphoma cells were positively stained with CD 30 ( Ki 1 / Ber H 2 ) , CD 4 ( helper T ) , and CD 25 ( interleukin 2 receptor ) antibodies . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The percentages of total B lymphocytes ( CD 19 ) , natural killer ( NK ) cells ( CD 16 ) , T lymphocytes and their subsets ( CD 2 , CD 3 , CD 4 , CD 5 , CD 7 , CD 8 ) , and IL 2R ( CD 25 ) were found to be within the range found in normal donors . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In addition , the effect of intraventricular fluid ( IVF ) on PHA stimulated in vitro CD 4 and CD 25 expression was investigated to determine whether severe head injury results in the production of mediators within the central nervous system capable of affecting T cell activation . ^^^ There was no significant change in PHA stimulated CD 4 or CD 25 expression following incubation of PBLs with intraventricular fluid ( IVF ) from head injured patients . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Due ti this basic need for information , we have studied diverse lymphocytic subpopulations in a normal population that serves as a reference group , using the following antigens : CD 3 , CD 4 , CD 8 , CD 20 , CD45RA , CD 25 , LAM 1 , CD 29 , CD11b and CD 23 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CR IIA had a normal human karyotype and expressed the surface markers CD 3 ( + ) , CD 4 ( + ) , CD 8 ( ) , CD 19 ( ) , CD 25 ( + ) and HLA DR ( + ) , confirming their helper T cell nature . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Furthermore , the factor significantly inhibited the expression of CD 3 , CD 4 , and IL 2 receptor ( IL 2R ) alpha chain ( CD 25 ) on PHA stimulated T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Surface expression of the activation marker CD 25 ( IL 2 receptor ) was also greatly increased , whereas CD 4 and CD 8 levels were not altered . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The surface phenotypes of 4D1D4 hybridoma cells were Thy 1 . 2+ , L3T4 ( CD 4 ) , Lyt 2 ( CD 8 ) , Asialo GM1+ and p 55 interleukin 2 ( IL 2 ) receptor ( CD 25 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Besides the parameters usually monitored for the evaluation of liver function and state of hepatic encephalopathy , immunological patterns such as lymphocyte subpopulations CD 3 , CD 4 , CD 8 , CD 16 ( NK ) , CD 25 and antibacterial activity against Ty 2 strain of Salmonella typhi were evaluated . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
On immunohistochemical examination , the majority of the tumor cells expressed CD 3 ( Leu 4 ) , CD 4 ( Leu 3 ) , HLA DR CD 30 ( Ki 1 / Ber H 2 ) , and CD 25 ( IL 2 receptor ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Useful methods for characterizing AIDS related non hodgkin ' s lymphomas include immunophenotypic studies using B cell differentiation and activation antigens ( HLA DR , CD 10 , CD 19 , CD 20 , CD 21 , CD 22 , CD 23 , CD 38 ) , evaluation of expression of surface immunoglobulins ( IgS ) , activation and proliferation ( CD 25 , CD 30 , CD 71 , Ki 67 ) , and identification of T cell markers ( CD 1 , CD 2 , CD 3 , CD 4 , CD 5 , CD 7 , CD 8 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Leukocyte antigens investigated were CD 2 , CD 3 , CD 4 ( two antibodies ) , CD 5 , CD 8 , CD 25 , CD 30 , CD 63 antigens and HLA DR ; HIV 1 encoded antigens were gag p 24 , pol reverse transcriptase , and env gp 41 and gp 120 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The great majority of CD 25 positive CBMC are activated CD 3 positive T lymphocytes in that most of them are HLA DR positive , coexpress both CD 4 and CD 8 antigens and at least a part of them display high affinity IL 2R . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Immunohistochemistry revealed changes in the expression of CD 1 , CD 3 , CD 4 , and CD 25 , particularly on perivascular cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
With a combination of 15 antibodies , including CD 2 , CD 3 , CD 4 , CD 5 , CD 7 , and CD 8 for T cell marker analysis , CD 10 , CD 19 , CD 20 , surface immunoglobulins , and cytoplasmic mu chain for B cell marker analysis , CD 13 and CD 33 for myeloid marker analysis , and HLA DR and CD 25 for other marker analysis , acute lymphoblastic leukemias of T cell type , cALL type , pre B cell type and B cell type , acute myeloid leukemias , acute unclassified leukemias and adult T cell leukemias could be clearly diagnosed by immunophenotyping of cell membrane molecules . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Consecutive sections were stained for T 1 ( CD 5 ) , T 4 ( CD 4 ) , T 8 ( CD 8 ) , TAC ( CD 25 ) , the delta chain markers delta TCR 1 and delta TCS 1 , and the beta chain marker beta F 1 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Induction of CD 23 , CD 25 and CD 4 expression on an eosinophilic cell line ( EoL 3 ) by interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) and interleukin 5 ( IL 5 ) . ^^^ The effects of IL 3 , GM CSF and IL 5 on the expression of CD 23 ( Fc epsilon RII ) , CD 25 ( IL 2R / p55 ) and CD 4 on an eosinophilic cell line ( EoL 3 ) were investigated by flow cytometry . ^^^ A separate incubation with IL 3 , GM CSF or IL 5 alone , did not induce the expression of CD 23 , CD 25 , or CD 4 . ^^^ After a further incubation for 6 days with IL 3 , GM CSF and IL 5 , CD 4 was then expressed , while CD 23 and CD 25 expression still increased . ^^^ Northern blot analysis revealed the presence of mRNA encoding CD 23 , CD 25 and CD 4 in EoL 3 stimulated by IL 3 , GM CSF and IL 5 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Similarly , changes in the expression of CD 3 , CD 4 , CD 8 , CD 57 , CD 16 and CD 25 cell surface antigens were independent or rIL 2 concentration and not altered by the presence of mismatched dsRNA . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The cell subsets assessed included natural killer cells , B cells , T cells , CD 4 and CD 8 subsets of T cells , and T cell subsets defined by the coexpression of markers that appear ( HLA DR , CD 25 , CD 38 ) or disappear ( CD45RA ) after activation . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Subsequent characterization of CD 4 T cells showed reduced expression of HLA DR and CD 25 ( IL 2 receptor alpha chain ) antigens later in SIV infection . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Peripheral T lymphocytes from 16 randomly selected patients with nonsegmental vitiligo were labeled with monoclonal antibodies recognizing T cell receptor ( TCR ) alpha / beta , TCR gamma / delta , CD 3 , CD 4 , CD 8 , CD45RA , CD45RO , CD11b , CD11c , CD 16 , CD 56 , CD 25 , CD 54 , and HLA DR antigens . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We previously reported that CD 25 ( IL 2R p 55 ) positive CD 3 CD4 CD 8 murine thymocytes can be induced to express CD 8 alpha ( Lyt 2 ) by transforming growth factor beta plus TNF alpha in the presence of IL 7 ( which is necessary to maintain the viability and differentiation capacity of CD25+CD3 CD 4 CD8 thymocytes in vitro ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The T cell line expressed CD 2 , CD 3 , CD 4 , CD45RA , CD 29 , HLA DR , CD 25 , and CD 26 antigens , but not CD 8 and CD 20 antigens . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
An increase in CD8+ cells and variable decreases in CD4+ cells have been found in the intestinal lamina propria by immunohistologic studies , resulting in a decreased CD 4 to CD 8 ratio ; in addition , CD 25 expression is reduced . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The parameters assessed were ( 1 ) percentages of lymphocytes expressing CD 4 and / or CD 25 ( interleukin 2 receptor ) , ( 2 ) serum levels of soluble CD 25 , ( 3 ) serostatus for other viruses , ( 4 ) anti HTLV antibody levels , and ( 5 ) HTLV type determined by polymerase chain reaction or serologic reactivity with type specific peptides . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Finally , while activated cells characterized by DR expression represented about 4 % of CD 4 and 9 % of CD 8 cells , CD 25 appeared to be poorly represented among CD 4 , CD 8 and CD20 . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Three years following the accident , exposed subjects were compared to residents of a nearby unexposed community for the following immunological parameters : white blood cell count , total lymphocyte count , percent and total lymphocyte subsets ( CD 5 , CD 4 , CD 8 , CD 19 , CD 25 , and CD 26 cells ) , prevalence of autoantibodies , and antibodies to HCHO human serum albumin ( HCHO HSA ) conjugate . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
When comparing the HTLV seropositive group ( N = 19 ) and a seronegative control group ( N = 20 ) , no significant differences were observed in either the proportions of the major lymphocyte subsets ( CD 3 , CD 4 , CD 8 , CD 19 , CD16 / 56 ) coexpressing CD 25 or the phenotypic distribution of CD25+ cells among these lymphocyte subsets . ^^^ Similarly , the total percentages of CD 3 , CD 4 , CD 8 , and CD 19 cell subsets were unchanged ; however , the percentage of CD16 / 56+ cells was significantly decreased in the HTLV group and reflected a decrease in the percentage of CD16 / 56 cells lacking CD 25 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Isoprinosine significantly enhanced the lymphoproliferative response after stimulation with phytohaemagglutinin ( PHA ) and purified derivative of tuberculin ( PPD ) , while isoprinosine had no effect on the following immune parameters : the expression of surface markers on blood mononuclear cells including CD 2 , CD 3 , CD 4 , CD 8 , CD 14 , CD 19 , CD 20 , CD 25 , leu 8 , and HLA DR . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Mononuclear cells from 16 HTLV seropositive former blood donors and 9 seronegative controls were cultured for 7 days ; activation was then assessed by measuring DNA synthesis in cultured cells and by monitoring CD 25 expression by CD 3 , CD 4 , CD 8 , CD 19 , and CD16 / 56 lymphocyte subsets . ^^^ Although neither the fraction of CD 4 cells expressing CD 25 nor the fraction of CD 25 cells expressing CD 4 were increased for the Prol + group , the modal fluorescence intensity value for CD 25 expression by CD 4 cells was increased , suggesting some CD 4 cell activation occurred as well . ^^^ Blastoid cells were , on average , 79 % CD 25 + , whereas the sum of CD 4 + CD 25 + ( 27 % ) , CD 8 + CD 25 + ( 30 % ) , CD 19 + CD 25 + ( 3 % ) , and CD16 / 56 + CD 25 + ( 35 % ) subsets was 95 % ; the presence of 17 % CD 8 + CD16 / 56 + cells accounted for most of this discrepancy . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
When expression of CD 25 was examined separately in CD 4 positive and CD 8 positive T cells and in B cells , there was a wider spread of values in the HIV group compared with controls , but no systematic difference . ^^^ In the patient group studied ( 110 samples from 53 patients ) there was a weak correlation between CD 25 and CD 4 expression ( correlation coefficient r = 0 . 21 , P less than 0 . 02 ) , but there were patients with low CD 25 and high CD 4 as well as patients with high CD 25 and low CD 4 , suggesting that CD 25 can vary independently of changes in CD 4 lymphocytes . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In the synovial tissue and synovial fluid of RA patients there was an increased percentage of cells expressing HLA DR , beta 2 microglobulin receptors , CD 25 , CD 5 , CD 4 , and Thy 1 , but the proportion of CD8+ cells was significantly decreased compared with that seen in peripheral blood . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Whereas CD 7 expression was increased 78 + / 25 % by 0 . 5 microM ionomycin , expression of CD 25 ( IL 2R alpha ) , class 2 MHC , 4F2 , transferrin receptor , CD 2 , CD 3 , CD 4 , CD 5 , and CD 8 molecules was not increased . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The phenotype and activation status of 5000 TILs were studied using Mabs to CD 4 , CD 8 , HLA DR , CD 25 ( the low affinity inducible IL 2 receptor ) and the transferrin receptor and related to Class 1 and Class 2 MHC expression on 5000 primary tumour cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Reactivity with the following antigens was assessed : MHC class 1 , 2 DR , DQ , and DP antigens ; leukocyte markers CD 1 , CD 2 , CD 3 , CD 4 , CD 8 , CD 14 , CD 16 , CD 25 , CD 57 ; a proliferation associated nuclear antigen ; interferon gamma and tumor necrosis factor alpha . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
By means of a panel of immunological reagents , including alloantisera and monoclonal antibodies against public domains of the T cell receptor , CD 4 , CD 8 , and the inducible interleukin 2 receptor light chain ( CD 25 ) , it is shown that the bulk of cells in the enlarged spleen are of host origin and do not express markers typical of mature T or B lymphocytes . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Extensive immuno cytochemical studies showed that the neoplastic cells were almost exclusively localized in the epidermis , expressed the gamma / delta variant of the T cell receptor ( CD3+ , TCR delta 1+ ) and were CD5+ , CD7+ , CD27+ , CD29+ , CD43+ , CD44+ , CD45+ , CD45RA+ , CD54+ , CD69+ , but beta F 1 , Ti gamma a , BB 3 , A 13 , CD 2 , CD 4 , CD 8 , CD11a , CD49d , CD 25 , CD 30 , and HLA DR . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
T lymphocytes form the peripheral blood of 12 patients with aplastic anemia ( AA ) and 4 patients with aplastic anaemia paroxysmal hemoglobinuria syndrome ( AA PNH ) were cultivated and their subsets were measured by monoclonal antibodies CD 4 , CD 8 , CD 25 with indirect immunofluorescence technique . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 25 is found on subsets of all thymocyte populations as defined by TcR alpha beta , TcR gamma delta , 2 , CD 4 , and CD 8 expression , cortical or medullary localization , and is also present on a subset of intrathymic nurse cell lymphocytes . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 3 , CD 4 , CD 16 and CD 57 antigens were weakly expressed , and the IL 2 receptor ( CD 25 ) was not detectable on these cells either before and after treatment with IL 2 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The distribution of cells expressing CD 3 , CD 4 , CD 8 , CD 14 , CD 16 , CD 19 and CD 25 markers was the same at 37 degrees and 39 degrees . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
TNF induced expression of CD 4 and CD 71 , increased the intensity of HLA DR , CD 25 , CD11c and CD 13 expression and decreased both the intensity and frequency of sIg and cIg positivity . alpha IFN decreased CD 25 expression , the tartrate resistant acid phosphatase activity ( TRAP ) , reduced the TNF induced CD 4 and CD 71 expression and antagonized the TNF effect on the Ig expression . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Soluble CD 8 , soluble CD 4 , soluble CD 25 ( IL 2 receptor ) , beta 2 microglobulin and the cytokine tumour necrosis factor alpha ( TNF alpha ) were measured in sera from patients with common variable immunodeficiency ( CVI ) . ^^^ Levels of soluble CD 8 , soluble CD 25 and beta 2 microglobulin but not of soluble CD 4 and TNF alpha were raised significantly above levels in normal sera . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Indirect immunofluorescence and monoclonal antibodies to leukocytic differentiated antigens CD 3 , CD 4 , CD 8 , CD 22 , CD 25 , CD 71 and HLA DR were used . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The two treatment groups did not differ from each other in respect to the number of CD 3 ( T 3 ) , CD 4 ( T 4 ) , CD 8 ( T 8 ) , CD 14 ( monocytes ) , CD 20 ( B cells ) or CD 25 ( interleukin 2 receptor positive cells ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The phenotype of these cells were as follows : Fc gamma R+ , CD2+ , CD 5 , CD 7 , CD 3 , CD 4 , CD 1 , CD 8 , sIg , CD 20 , CD 11 , CD 13 , OKIa+ , CD 25 , CD16+ , Leu 7 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
By using an immunogold silver ( IGS ) enhancement method to label cell surface antigens , it was possible to detect the presence of CD 3 , CD 4 , CD 25 and OKT 11 marker in 55 % , 54 % , 77 % , and 71 % of the IFN gamma producer cells , respectively . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
A majority of the cells from peripheral blood coexpressed the antigens against MAbs CD 2 , CD 3 , CD 4 , CD 5 , Ti ( WT 31 ) , CD 25 , CD 38 , CD 45 , and CD 29 , but did not express the antigens against CD 1 , CD 13 , CD 14 , CD 33 , CD 36 , CD 10 , CD 19 , CD 20 , CD 21 , CD 24 , CD 41 , CD 42 , CD45RA , CD 56 , and CD 57 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Other cell membrane markers , such as CD 2 and CD 25 , remain constant throughout the course of infection ; after its initial decrease , CD 4 remains at 70 % of control values . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Using this method we tested which T cell sub subpopulation is responsible for the weak CD 25 ( IL 2R alpha ) expression constantly associated with freshly isolated human T cells and concluded that it is predominantly expressed on the CD4+ CD45RO+ ( CD 4 positive memory ) T cell subpopulation . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
With 18 patients afflicted with recently diagnosed , non leukemic stages of B and T cell lymphoma cytofluorimetric analysis was performed with peripheral blood lymphocytes ( PBL ) after 72 h in culture with and without phytohemagglutinin , using antibodies against the differentiation antigens CD 3 , CD 8 , CD 4 , CD 16 , CD 19 , CDw 14 , and the activation antigens interleukin 2 receptor ( IL 2R , CD 25 ) , HLA DR ( DR ) , CD 56 and transferrin receptor ( TR ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In the present study a comparative immunohistochemical study was performed on skin biopsies from of patients with Jessner ' s lymphocytic infiltration of the skin ( LIS ) , polymorphous light eruption ( PLE ) , discoid lupus erythematosus ( DLE ) and subacute cutaneous lupus erythematosus ( SCLE ) using a large panel of monoclonal antibodies against T cell differentiation antigens ( CD 3 , CD 4 , CD 8 ) , immunoregulatory T cell subsets ( CD 7 , 4B4 , 2H4 , Leu 8 ) , B cells ( CD 22 ) , activated cells CD 25 , OKT 9 , HLA DR ) , Langerhans cells ( CD 1 ) and macrophages ( Leu M 5 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Antibodies to the following cluster of differentiation ( CD ) antigens were used : CD 3 , CD 4 , CD 8 ( T cell markers ) , CD 11b , CD 14 ( macrophage marker ) , CD 16 [ an antigen expressed by natural killer ( NK ) cells and granulocytes ] , and CD 25 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In this report it is shown by immunofluorescence analysis , biochemical analysis and mRNA hybridization that human eosinophils express surface CD 4 and interleukin 2 receptor ( IL 2R ) ( CD 25 ) when exposed to eosinophil activators granulocyte macrophage colony stimulating factor ( GM CSF ) and IL 3 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
PHA was mostly mitogenic for T PBL bearing the CD 3 , CD 5 , CD 7 , CD 8 and CD 25 differentiation clusters , and a low density of CD 1 and CD 4 had a small effect on human natural killer cells ( HNK ) and also did not stimulate B ( CD 19 ) and HLA DR+ PBL . ^^^ The mitogenic effect of PWM was evident on CD 3 , CD 5 , CD 7 , CD 4 , CD 25 and CD 8 , but not on HNK , HLA DR and CD 19 B PBL , which presumably had already differentiated into antibody secreting cells . ^^^ After MLC stimulation all T , B and HNK PBL subsets tested were increased , but the cells bearing CD 1 , CD 4 , CD 5 , CD 7 , CD 25 , HNK , CD 19 and HLA DR had the greatest proliferation with respect to the unmixed control PBL . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
These subsets included B , T , CD 4 , and CD 8 cells and subpopulations of CD 4 and CD 8 cells defined by the coexpression of markers that appear ( CD 25 , HLA DR , CD 38 ) or disappear ( Leu 8 , CD45RA ) after activation . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
IL 2 treatment did not induce PBL proliferation , or changes in the expression of CD 25 ( Tac ) , HLA DR , CD 38 , CD 4 , CD 8 , CD 57 , or OKB 7 in the patients ' PBL . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In parallel , cytofluorimetric analysis was done with PBL after 72 hours in culture with and without PHA , using antibodies against the differentiation antigens : CD 3 , CD 8 , CD 4 , CD 19 , and CDw 14 and the activation antigens : interleukin 2 ( IL 2 ) receptor ( IL 2R , CD 25 ) , human leukocyte antigen DR ( HLA DR ) , and transferrin receptor ( TR ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In functional assays , anti CD 2 , anti DR 6 15C , and anti CD 25 MoAbs blocked the proliferative response to DT and anti CD 3 , anti CD 4 , anti D 44 MoAbs had intermediate inhibition effects . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
S 100 beta+ T cells recognized by their diffuse intracytoplasmic immunoperoxidase reaction , also expressed CD 2 , CD 3 , CD 8 antigens demonstrated by surface blue alkaline phosphatase reactivity , but not CD 4 , CD 1 , CD 25 ( interleukin 2 receptor ) , or HLA DR antigens . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The percentage of cells expressing IL 2 receptors ( CD 25 ) was also normal both after addition of PHA and after addition of A23187 / PMA and the expression was normal on both CD 4 and CD 8 cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The immunolabelling study confirmed this diagnosis , demonstrating the following phenotype CD 4 , CD 25 ( TAC ) , CD 30 ( Ki 1 ) , OKT 9 ( transferrin receptor ) , EMA and HLA DR positivity . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
To investigate whether the presence or absence of tissue eosinophilia is related to the immunophenotype of the T cells , we studied 43 cases of HD ( 28 nodular sclerosing , ten mixed cellularity , and five unclassifiable ) for the relative numbers of lymphocytes positive for CD 2 , CD 3 , CD 4 , CD 5 , CD 8 , CD 25 , CD 38 , T 9 , TQ 1 , HLA DR , and beta F 1 , and for the number of eosinophils in tissue sections . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
At 48 h , lymphocytes were more numerous than macrophages in both groups , CD 8 lymphocytes were more abundant in CRS reactions , but CD 4 and CD 25 ( activated ) T lymphocytes and macrophages had a similar density in both groups . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
After short term culture without an addition of any differentiation stimulators , the blast cells expressed CD 2 , CD 3 , CD 4 , CD 8 , and CD 25 antigens . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In this study the following monoclonal antibodies ( MoAb ) were used : Leu 2a ( CD 8 ) , Leu 3a / 3b ( CD 4 ) , Leu 4 ( CD 3 ) , anti HLA DR and anti IL 2 R ( CD 25 ) , OKM 1 ( CD 11 ) , OKB 2 ( CD 24 ) and 1HT4 4H3 ( CD 25 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Classical markers of T cell activation ( CD 25 , HLA D ) and proliferation were absent , and strong expression of the CD 7 `` immunostimulation ' ' marker was approximately equal in both CD 4 and CD 8 subsets . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Leucocyte subsets were enumerated with a panel of monoclonal antibodies which included CD 3 , CD 4 , CD 8 , TQ 1 , Leu 7 , CD 15 , HLA DR , CD 25 , CD 22 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Granulated lymphoid cells ( CD2+ , CD7+ , CD38+ , NKH1+ , CD 3 , CD 5 , CD 4 , CD 8 , CD 25 ) are prominent in human endometrial stroma in the late secretory phase of the menstrual cycle and in early pregnancy , and may play an important role in implantation and placentation . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The surface marker analysis revealed that CD 3 , CD 4 , CD 8 and CD 25 positive cells were 88 . 1 , 83 . 9 , 6 . 4 and 1 . 3 % of the peripheral mononuclear cells , respectively . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Five cases of prescalenic granulomatous lymphadenitis ( GL ) and 2 cases of prescalenic nongranulomatous lymphadenitis ( NGL ) in 7 patients with sarcoidosis were studied with a large panel of monoclonal antibodies ( anti CD 1 , CD 3 , CD 4 , CD 8 , CD 14 , CD 22 , CD 25 , HLA DR , HLA DQ , HNK 1 , R 4 / 23 ) . ^^^ Intra and intergranulomatous T lymphocytes were mainly of the CD 4 type in 4 out of 5 cases ; in all the cases less than 25 % of T lymphocytes stained also for CD 25 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
TPA induced the differentiation of the more mature pre T ALL cells of this case in vitro , and not only CD 25 ( Tac ) antigen but also CD 4 and CD 8 antigens appeared on the cell surface . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Immunolabeling experiments showed that resident Thy 1+DEC are CD45+ , Thy 1+ , CD3+ , TcR 5 beta 8 , CD 5 , CD 4 CD 8 , CD 25 lymphocytes . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We tested the ability of these heterodimers to direct a CD 2 + CD 3 + CD 8 + CD 4 CD 25 + transferrin receptor + MHC restricted human cytolytic T lymphocyte ( CTL ) clone to lyse a CALLA + human tumor in vitro . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In the present study , the leukocyte infiltrates in the affected skin of EDP and vitiligo patients were studied using the avidin biotin ( ABC ) immunoperoxidase technique and monoclonal antibodies which recognise the following mononuclear cell subgroups : T suppressor / cytotoxic ( CD 8 Leu 2 ) , T helper ( CD 4 = OKT 4 ) , T suppressor + macrophages ( Leu 15 ) , Pan T ( CD 3 = Leu 4 ) , macrophages ( Leu M 3 ) and Langerhans cells ( CD 1 = Leu 6 ) , and other cellular markers such as Ia antigens and the Interleukin 2 receptor ( CD 25 = TAC ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
This response was noted as soon as 2 h after addition of rIL 2 . 2 h rIL 2 activated plastic adherent lymphocytes were 90 98 % LGL , expressed surface markers characteristic of rat NK cells ( OX 8 [ CD 8 ] + , asialo GM 1 , laminin+ , OX 19 [ CD 5 ] , R 1 3B3 [ CD 5 ] , W3 / 25 [ CD 4 ] , OX 39 [ CD 25 ] , Ia , and Ig ) , and expressed very high levels of cytotoxicity against YAC 1 target cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We report a case of Ph 1 negative , bcr negative CML BC , in which the primary leukemic cells displayed T related antigens ( CD 7 , CD 4 ) in addition to HLA DR and CD 25 determinants . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
With our system , a wide variety of antigens are detected in plastic sections without trypsinization or prolonged embedding procedures ; pan B ( CD 19 , CD 22 ) , pan T ( CD 7 , CD 5 , CD 3 , CD 2 ) , T subset ( CD 4 , CD 8 , CD 1 , CD 25 ) markers as well as surface immunoglobulin and markers for myeloid and mononuclear phagocyte cells are preserved . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Phenotypic analysis showed that the hybrids could be grouped as follows : a high frequency expressing CD 25 ( IL 2 receptor ) , human T 200 , HLA class 1 alpha and beta 2microglobulin , and reacting with the mAb H 207 and 12E7 ; an intermediate frequency expressing CD 1 and CD 2 ; and a low frequency expressing CD 3 , CD 4 , CD 5 , CD 7 , CD 8 and CD 9 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Immunohistological analysis with a range of monoclonal antibodies showed the PTC of our case to be CD 5 ( T 1 ) + , CD 4 ( T 4 ) + , CD 3 ( T 3 ) , CD 8 ( T 8 ) , CD 2 ( T 11 ) , and CD 25 ( TAC ) , but HLA DR+ and transferrin receptor positive . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We observed no changes in the percentage of CD 3 , CD 4 , CD 8 and CD 25 positive lymphocytes in both MTX and NSAID treated groups . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Both CD 4 and CD 8 T cell subsets within MC cultures are activated during SLP , as judged by high density CD 25 ( CD25bright ) expression ; it is unclear , however , whether both cell subsets can directly undergo SLP . ^^^ However , inhibition of CD 8 or CD 4 cell SLP by cyclosporin A or anti Tac ( anti CD 25 ) did not reduce Ag production , indicating that Ag production is not coupled to SLP . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Lymphocyte subpopulations including CD 2 , CD 3 , CD 4 , CD 8 , CD 19 , CD 20 , and CD 25 were also measured , rhG CSF was administered once daily as a 30 min infusion . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Surface marker analysis revealed the blasts to be CD 2 , CD 3 , CD 4 , CD 5 , CD7+ , CD 8 , CD 10 , CD 13 + / , CD 19 , CD 20 , CD 25 , CD33+ and human leukocyte antigen DR ( HLA DR+ ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Significant increases were seen in the proportion of PBMC expressing natural killer ( NK ) antigen CD 56 ( 14 . 7 + / 2 . 4 % versus 18 . 1 + / 2 . 6 % ; P < 0 . 05 ) and surface IL 2R ( CD 25 ; 0 % versus 0 . 42 + / 0 . 15 % ; P < 0 . 05 ) , in sIL 2R ( 314 + / 86 U / ml versus 736 + / 173 U / ml ; P < 0 . 05 ) , and in the CD 4 : CD 8 ratio ( 2 . 34 + / 0 . 93 versus 3 . 47 + / 1 . 23 ; P < 0 . 01 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
When fetal thymic ontogeny was examined , we found a marked difference between zeta / embryos and heterozygous littermates from embryonic day 17 . 5 , in terms of CD 25 , CD 4 and CD 8 expression , and thymus size . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In the immunohistochemical study , antibodies against 8 lymphocyte differentiation antigens , including CD 4 , CD 8 , CD 3 , CD 1 , CD 2 , CD 25 , CD 22 and T 9 as well as those against keration and S 100 protein were used . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
PMT 2Y cells are positive for CD 2 , CD 3 , CD 4 , CD 25 , T cell receptor alpha beta and HLA DR , but negative for CD 1 , CD 7 , CD 8 , CD 19 and CD 20 , indicating that the clone belongs to a helper / inducer subset of T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In addition , a time course of the effects of E 2 on circulating adhesion molecules ( AMs ) , cortisol serum levels , differential blood counts , and surface expression of the lymphocyte function associated antigen 1 ( CD11a / CD18 ) , CD 3 , CD 4 , CD 19 , and CD 25 on leukocytes was studied in another group of volunteers . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Ascites and peripheral blood of 12 patients with advanced ovarian cancer ( stage 4 ) have been investigated by two color flow cytometry for leukocytes and lymphocyte subsets with monoclonal antibodies , against CD 3 , CD 4 , CD 8 , CD 14 , CD16 / 56 , CD 19 , CD 25 , CD 45 , CD 57 , and HLA DR . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Tissue sections were tested by peroxidase antiperoxidase method , using the following monoclonal antibodies : OX 19 ( CD 5 ) , W3 / 25 ( CD 4 ) , OX 8 ( CD 8 ) , OX 6 ( Ia ) , OX 33 ( LCA ) and OX 39 ( CD 25 ) . ^^^ Few T lymphocytes ( CD5+ ) were found in arthritic synovial membrane , whereas an increase of cells expressing CD 8 , CD 4 , CD 25 , Ia , and LCA was found . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In the absence of gp 120 , CD 4 displayed high association with CD 3 , CD 5 , CD45RC , CD 25 , CD 28 , CD 44 , and CD 53 ; weak association with CD 2 , CD 38 , CD45RB , CD62L , and CD 26 ; and no association with CD45RA , CD45RO , CD11b , CD11a , CD 54 , CD 7 , CD 48 , CD 98 , CD 59 CD55 , HLA class 1 and class 2 molecules . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
With prolonged culture , CD1a was downregulated on a major population of cells , paralleled by a loss of BG and an increase of CD 4 , CD 25 , and CD 80 expression that may correspond to the maturation of epidermal LC in vitro . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Subpopulations of alveolar macrophages separated using discontinuous Percoll density gradient centrifugation and T lymphocytes ( with CD 3 , CD 4 , CD 8 , and CD 25 monoclonal antibodies ) were quantified . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In our first study , 157 patients in CDC groups 2 4 were examined cross sectionally for in vivo expression of the activation markers HLA DR and CD 25 on CD 3 , CD 4 and CD 8 T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Alterations in the expression of cell surface receptors have been reported in HIV infected cells for CD 4 , CD 25 ( IL 2 receptor ) , CD 2 , CD 3 and CD 8 and CD 26 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In addition , the peculiar immunophenotype of infiltrating cells ( CD 3 , CD 4 , CD 8 , CD 25 ) and their correlation with HLA DR expression before and after treatment with oral cetirizine , a highly selective , third generation H 1 receptor antagonist has been examined using the labeled avidin biotin ( LAB ) system . ^^^ The numbers of infiltrating CD 3 , CD 4 , CD 8 positive cells were reduced , whereas there was no significant modification of CD 25 positive cells within the epidermis and the dermis . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
On immunohistochemical examination , Ki 1 , HLA DR , CD 25 ( IL 2 receptor alpha ) , CD 122 ( IL 2 receptor beta ) , CD 4 , CD11c and CD 68 were all positive in the tumor cells , whereas CD1a , CD 3 , CD 5 , CD 8 and CD 19 were negative . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Lymphocytes were also characterized for the expression of CD 4 and CD 8 T cell markers and , within each population , for the expression of activation markers ( CD 25 , CD 69 ) and adhesion markers ( CD 51 , CD 54 , CD11b , CD49e , L selectin ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The presence of T cell markers ( CD 2 , CD 3 , CD 4 , CD 8 in our cases ) and antigens of cell activation ( CD 25 , HLA DR ) were found . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Most of these lymphocytes expressed CD 3 , CD 4 , CD45RO , and TCR alpha beta , whereas less than 5 % of them expressed CD 25 and CD 69 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
During the process of maturation , macrophage precursors gradually lost their MHC class 2 expression , acquired other surface markers ( CD 45 , Thy 1 , CD 25 , CD 4 , etc . ) and increased the acid phosphatase activity . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
It has been reported that both CD4lo ( CD44+ CD 25 c kit+ CD 3 CD4lo CD 8 ) and pro T cells ( CD44+ CD25+ c kit+ CD 3 CD 4 CD 8 , representing the next step in maturation ) exhibit germline T cell receptor beta and gamma loci , suggesting that neither population is exclusively committed to the T cell lineage . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The amount of TNF alpha released correlated positively with the percentage expression of CD 4 ( r = 0 . 72 ) and CD 25 ( r = 0 . 70 ) by lymphocytes , and of CD 14 ( r = 0 . 63 ) , VLA 4 ( r = 0 . 59 ) , FRD 1 ( r = 0 . 67 ) and 27E10 ( r = 0 . 67 ) by AM , with p < 0 . 001 for all correlations . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We evaluated the infiltrating mononuclear cells in both epithelium and lamina propria for expression of CD 2 , CD 4 , CD 8 , CD 25 , T alpha / beta and gamma / delta receptors , CD 16 , CD 56 , CD 57 and also studied the distribution of cell adhesion molecules ( ELAM 1 , VCAM 1 , ICAM 1 , PECAM 1 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
This MFI declined once the CD 4 expressed HLA DR in PB of SS patients ( P < 0 . 004 ) and normal controls ( P < 0 . 02 ) or CD 25 in PB of RA ( P < 0 . 004 ) and SS patients ( P < 0 . 0004 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Flow cytometry analysis revealed that these cells express CD 45 , CD 56 , CD 2 , CD 7 , CD11a , CD 18 , CD 69 and the intermediate affinity ( p 70 ) IL 2 receptor ( IL 2R ) but not CD 57 , CD 16 , CD 3 , CD 4 , CD 5 , CD 8 , CD45RA , CD 25 , or the high affinity p 55 IL 2R . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Peripheral blood T lymphocytes and IL 2R expression were analysed using fluorescein isothiocyanate labelled CD 3 , CD 4 , CD 8 and CD 25 monoclonal antibodies with flow cytometry . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The phenotype of the major population of embryonic day 12 thymus cells was HSA+ , CD44+ , c kit+ , Thy 1 , CD 25 , CD 4 , CD 8 , TcR , and Sca 1 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 and CD 25 monoclonal antibody cocktail in kidney transplant rejection prophylaxis : clinical results of a pilot study . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
These markers consist of T cell specific antigens ( CD 2 , CD 3 , CD 4 , and CD 8 ) , activated T cell antigens ( CD 25 and CD 71 ) , major histocompatibility complex ( MHC ) antigens ( class 1 and 2 ) , and adhesion molecules ( LFA 1 and ICAM 1 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The subset distribution ( CD 3 , CD 4 , CD 8 , CD 16 , DR ) was assessed by cytofluorometry with specific monoclonal antibodies ( MAbs ) ; the p 55 ( CD 25 ) and p 75 subunit expression was evaluated by specific MAb ( OKT26a and anti p 75 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The cells positive to antigens CD 3 , CD 4 , CD 8 , HLA DR , CD 19 , CD 25 , CD 57 , CD 16 , and CD 14 , and the cytotoxic activity of the lymphocytes on target cells K 562 were assessed by cytofluorimetric analysis . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Also , both trophoblast and decidual cells caused no significant changes in the expression of Thy 1 , CD 5 , CD 4 , CD 8 , CD 3 , CD 25 , CD 44 , and L selectin by the thymocytes . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The malignant cells expressed the CD 30 ( Ki 1 ) , CD 3 , and CD 4 antigens , the beta chain of the interleukin 2 receptor ( CD 25 ) , and the betaF 1 antigen ( alpha / beta T cell receptor ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In the abnormal lymphocyte cells surface marker test by flow cytometry showed that they expressed CD 2 , CD 3 , CD 4 , CD 29 , CD45RA , and CD 38 , but not CD 8 , CD 16 , and CD 25 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Double fluorescence activated flow cytometry was used to assess HML 1 expression with T cell subtype antigens ( CD 3 , CD 4 , CD 8 ) or activation markers interleukin 2 receptor ( IL 2R ) ( CD 25 ) , HLA DR , and lymphocyte function associated antigen ( LFA 1 ) ( CD11a ) were assessed by flow cytometry . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The immunophenotypical analysis of lymphoma revealed that the tumor expressed CD 2 , CD 3 , CD 4 , CD 5 , CD 25 and HLA DR . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Using such agents , donor specific tolerance has been induced in experimental models after a limited course of therapy directed against selected effector cell surface associated molecules such as CD 4 , CD 25 , and CD 54 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The percentages of gated lymphocytes expressing CD 3 , CD 8 , CD 19 , and HLA DR , but not CD 2 , CD 4 , CD 16 , and CD 25 , were significantly lower in FITC stained versus PE stained preparations . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
During the repeat provocation there was no difference in numerical values , with respect to any of the five leukocyte subpopulations studied ( CD 4 , CD 8 , CD 25 , CD45RA and CD45RO ) , between patients and controls . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We have investigated antibodies against CD 4 , CD 8 , CD 20 , CD 23 and CD 25 in frozen sections of human nasal mucosa ; however , the methodology of standardization is of general validity . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
T helper ( CD 4 ) cells in the lamina propria show a non proliferative activation ( CD 25 ) which can be induced after gluten challenge in vitro . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Flow cytometry was used as a quantification technique to measure lymphocyte subpopulations , CD 3 ( T cells ) , CD 19 ( B cells ) , CD 16 ( natural killer cells ) , CD 4 ( T helper / inducer cells ) , CD 8 ( T cytotoxic / suppressor cell ) , CD 25 ( interleukin 2 receptor ) , CD 69 ( activation inducer molecule marker ) , CD 71 ( transferrin receptor ) and CD 54 ( ICAM 1 ) ; CD4 / CD8 ratios were also estimated . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Immunophenotypic analysis showed that they were positive for CD 3 , HLA DR , CD 25 and CD 30 , but negative for CD 4 , CD 8 , CD 19 , FVIII and GpIIb / IIIa indicating Ki 1 positive lymphoma with T cell lineage . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Three colour staining combining a polyclonal antibody reagent to CD 3 and a monoclonal antibody to CD 25 with a monoclonal antibody to CD45R0 , CD 4 , CD 8 , the p 75 beta chain of interleukin 2 receptor , integrin alpha E beta 7 , or HLA DR showed that most of the CD25+ T cells ( > 90 % ) were CD4+CD8 , co expressed CD45R0 and the p 75 beta chain , and often also the integrin alpha E beta 7 but not HLA DR . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Our results demonstrate that whereas expression of major histocompatibility complex ( MHC ) molecules , CD 45 , Mac 1 , LFA 1 , B cell markers , macrophage markers and some T cell markers ( as CD 2 , CD 4 and CD 8 ) did not undergo changes in culture , the level of expression of the adhesion molecules VLA 4 and ICAM 1 , and the T cell markers CD 5 , CD 25 and Thy 1 increased after 14 h incubation at 37 degrees C . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Although gamma delta T cells could not proliferate on their own in the absence of CD 4 T cells ( or exogenous IL 2 ) , the appearance of IL 2 receptors ( CD 25 ) was triggered in the absence of CD 4 T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The blasts were positive for CD1a , CD 4 , CD 16 , CD 35 , HLADR , HLADQ , CD11b , CD11c , CD 14 , CD 33 , CD 34 , CD11a , CD 71 , CD 19 , CD 25 , IL 2R beta and negative for CD 2 , CD 7 , CD 8 , CD 10 , CD 22 , CD 56 , CD 57 , surface or cytoplasmic CD 3 , TCR delta and TCR beta , HTLV 1p19 and P glycoprotein . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In supporting the existence of thymic DC subpopulations , we also demonstrated a differential expression of various cell markers , including CD 4 , CD 8 , CD 25 , adhesion molecules and the antigen recognized by OX 44 monoclonal antibody ( mAb ) , on thymic DC during both embryonic and adult life . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We studied the lymphocyte activation markers CD 25 ( interleukin 2 receptor ) , CD 38 , CDw49b ( VLA 2 ) , CD 71 ( transferrin receptor ) , and HLA DR , as well as the surface antigens CD 3 , CD 4 , CD 7 , and CD 8 by two color flow cytometry in 23 chronic HD patients before and after a single dialysis session ; we also studied 30 normal controls . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Eosinophils from eosinophilic patients became hypodense compared to their pre treatment levels as demonstrated by sedimentation through a discontinuous metrizamide density gradient ; they also showed an increased expression of CD 4 , CD 25 and CD11b cell surface activation markers . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Cryostat sections from retrobulbar tissues of 15 GO patients and 11 controls were analyzed with a battery of monoclonal antibodies against CD 2 , CD 4 , CD 8 , CD11a , CD19 / 22 , CD 25 , CD 54 , CD 57 , CD 68 , C3b , HLA A , B , C , and HLA DR . ^^^ In contrast to controls , the retrobulbar adipose tissue showed an increase of HLA DR expression , an activation of intercellular adhesion molecule 1 ( ICAM 1 , CD 54 ) , as well as a marked infiltration of activated T cells ( especially CD 4 ) and macrophages ( CD 68 ) , whereas a positive staining for interleukin 2 receptors ( CD 25 ) , T cells ( CD 2 ) , B cells ( CD19 / 22 ) , complement factors ( C3b ) , and LFA 1 ( CD11a ) was present only in a few patients ' specimens . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
A faster rate of activation by gamma delta T cells compared to either CD 4 or CD 8 T cells was seen in the time course of IL 2 receptor alpha chain ( CD 25 ) cell surface expression . ^^^ All gamma delta T cells expressed the CD 25 protein within 8 hr of activation whereas the majority of CD 4 or CD 8 T cells did not express CD 25 until 24 hr post concanavalin A ( Con A ) stimulation . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Leukocyte and differential counts ; percentages of lymphocyte subpopulations ( CD 3 , CD 4 , CD 8 , CD 16 , CD 20 , CD 25 , and B 5 positive lymphocytes ) and monocytes ( CD 14 ) ; phytohemagglutinin , concanavalin A , and pokeweed mitogen induced and unstimulated proliferation of separated lymphocytes ; unstimulated and pokeweed mitogen stimulated production of IgG , IgM , or IgA ; and serum interleukin 6 , interleukin 1 beta , and serum C reactive protein concentrations were measured preoperatively and on postoperative Days 1 , 7 , and 21 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Soluble CD 4 , soluble CD 8 , soluble CD 25 , lymphopoieitic recovery , and endogenous cytokines after high dose chemotherapy and blood stem cell transplantation . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Expression of the activation markers CD 25 , HLA DR and VLA 1 and the CD 45 isoforms CD45RA and CD45RO on peripheral blood CD 4 and CD 8 T lymphocytes was measured using dual fluorescence flow cytometry . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In contrast , TGF beta 1 did not influence the constitutive or activation induced expression of CD 4 , CD 8 , CD45RA , CD45RO , CD 25 , CD 71 , CD 54 , CD 58 , CD 59 , and B 7 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Cellular immunity data did not differ significantly between groups , but alterations occurred at day 14 to 15 after surgery : CD 3 , CD 4 and ratio CD 4 : CD 8 subsets of cells showed minimum values ; CD 8 , CD4CD8 , CD 25 and white blood cell counts were at maximum at this time . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Immunohistochemical studies showed that the neoplastic cells expressed the gamma / delta T cell receptor ( CD3+ , C gamma M1+ ) and were CD2+ , CD43+ , CD45+ , CD45RO+ , PCNA+ , but were beta F 1 , CD 1 , CD 4 , CD 8 , CD 15 , CD 20 , CD 25 , CD 30 , CD45R , CD 57 , CD 68 , Mac 387 , and HLA Dr . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The other surface markers including classical sialyl Le ( 10 ) antigen defined by SNH 3 or FH 6 and LFA 1 , VLA 4 , CD 4 , CD 25 , ICAM 1 , Leu 8 , and HLA DR did not show a significant difference regardless of skin involvement . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
These observations indicate that sCD 4 and sCD 25 in the CSF are probably associated with meningeal infiltration of leukaemia cells that expressed CD 4 and CD 25 on the surface membrane and new markers for the meningeal infiltration of ATL cells . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Encouraging results have been so far obtained with : a ) cyclosporine A , a somewhat more specific immunosuppressive agent ; b ) monoclonal antibodies against surface antigens ( CD 4 , CD 5 , CD 7 , CD 25 , CD 54 ) expressed on activated T cells ; c ) T cell vaccination ; and finally ( i . v . ) recombinant cytokines , their agonists or antagonists . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Other non B cell lineage markers such as myeloid ( CD 13 , CD 14 , CD 15 , CD 33 , CD 41 , and glycophorin A ) , T cell ( CD 2 and CD 4 ) , and natural killer associated ( CD 56 ) antigens , as well as CD 23 , CD 24 , CD 25 , CD 37 , CD 39 , CDw 40 , CD45R , CD 71 , and certain unclustered antigens ( R 1 3 , PCA 1 , PCA 2 , PC 1 , 62B1 , 8A , 8F6 , and MM 4 ) , have been noted in myelomatous PCs . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Increased IL 2R expression ( CD 25 ) on CD 4 lymphocytes was noted in groups 1 , 2 and 3 implying activation of these cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Expression of HLA DR antigens and infiltration of T lymphocyte subsets ( CD 4 , CD 8 ) , cell activation marker ( CD 25 ) , B cells ( CD 20 ) , macrophages ( CD 68 and Ber Mac 3 ) and natural killer cells ( CD 56 ) in the rectal mucosa of patients with bacillary dysentery and in healthy controls were studied in an effort to interpret the immunopathological changes taking place in the rectal mucosa during the acute phase of shigellosis . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
There was no demonstrable alteration in the percentage of cells positive for CD 3 , CD 4 , CD 8 , CD 25 or CD 56 in peripheral blood or urine populations in six patients treated with intravesical IL 2 , or the pattern of MHC class 1 or 2 expression on tumour biopsies before and after treatment . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
RESULTS Compared with normal control marrows ( n = 23 ) no major abnormalities of T cells ( CD 3 ) , T cell subsets ( CD 4 , CD 8 , CD 25 , TCR gamma / delta ) or natural killer cells ( CD 56 , CD 57 ) were seen in the 65 patients . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Percentages of the different lymphocytes subsets , including CD 2 ( T cells ) ; CD 4 ; CD 45 RA ( suppressor inducer / virgin T cells ) ; CD 4 CD29 ; CD 8 ; CD 19 ( B cells ) as well as interleukin 2 ( IL 2 ) receptor ( CD 25 ) and class 2 antigen ( HLA DR ) expression , were analyzed by flow cytometry . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Synovial fluid and peripheral blood mononuclear cells were analysed for cell surface expression of CD 2 , CD 3 , CD 4 , CD 8 , CD 19 , CD 25 , CD 29 , CD45R and Ia using flow cytometry . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Human immunodeficiency virus type 1 ( HIV 1 ) infected H 9 and blood mononuclear cells ( MNCs ) were studied by immunogold electron microscopy for the presence of HIV 1 gag p 24 protein , env gp 41 and gp 120 proteins , and host cell molecules CD 4 , CD11a , CD 25 , CD 54 , CD 63 , HLA class 1 and HLA DR . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Expression of CD 25 , human lymphocyte antigen ( HLA ) DR , CD45RA , and CD45RO on CD 4 and CD 8 T lymphocytes was measured by flow cytometry , and serum concentrations of interleukin 5 ( IL 5 ) were measured using an enzyme linked immunosorbent assay technique . ^^^ On Day 1 the asthmatic patients showed significantly higher percentages , as compared with the control subjects of CD 4 T lymphocytes expressing the markers CD 25 , HLA DR , and CD45RO and significantly lower percentages of CD 4 T cells expressing CD45RA . ^^^ After glucocorticoid therapy , the percentages of CD 4 T cells expressing CD 25 , HLA DR , and CD45RO were significantly reduced in the asthmatic patients , and the percentages of those expressing CD45RA significantly increased so that by Day 7 expression of all four markers was no longer significantly different from that of the control subjects . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The efficacy of this method was evaluated by analyzing leukocytes of 42 healthy donors for the following markers : CD 3 , CD 4 , CD 8 , CD 14 , CD 16 , CD 19 , CD 25 , CD 38 , CD45RO , CD45RA , CD 56 , CD 57 , TCR gamma / delta and HLA DR . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In order to define the role of immune activation in vivo , we assessed the expression of the T cell activation markers HLA DR and CD 25 by flow cytometry in peripheral blood in relation to disease severity and the surrogate markers CD 4 and beta 2 microglobulin in 157 patients with HIV infection and 53 healthy seronegative blood donors . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Activated and resting CD 4 T cells were similarly reduced , the expression of CD 25 and HLA DR of CD 8 T cells was unaltered and increased , respectively . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
To reveal the association between gamma / delta TCR of blood T lymphocytes and the accessory molecules , expression of CD 4 , CD 8 and CD 25 were determined . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Biopsy specimens were strained with the indirect immunoperoxidase technique and monoclonal antibodies to CD 3 , CD 4 , CD 8 , CD 22 , and CD 25 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Synovial cells bearing CD 3 , CD 4 , CD 8 , CD 19 , LeuM 5 ( CD11c ) , HLA DR , HLA DP and CD 25 antigens were measured by stereology . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Their phenotypic repertoire was performed by FACS analysis using the following monoclonal antibodies : CD 3 , CD 19 , CD 4 , CD 29 , CD 18 , CD 16 , CD 56 , CD 25 , TCR alpha / beta , TCR alpha / delta . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Intravenous immunoglobulins , cytokines and anticytokines , immunotoxins , monoclonal anti leucocyte antibodies ( CD 4 , CD 25 , LFA 1 ) and soon peptide vaccination constitute new types of treatments which are now being evaluated . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
These were compared with the proportion of CD 4 CD45RO , IL 2R ( CD 25 ) , and transferrin receptor ( CD 71 ) positive peripheral blood lymphocytes . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In addition , PBMCs from these patients were phenotyped after in vitro MoAb stimulation with antibodies against CD 2 , CD 3 , CD 4 , CD 8 , CD 16 , CD 20 , CD 25 ( interleukin 2 receptor [ IL 2R ] ) , CD45RA , and UCHL 1 , and the production of interleukin 2 ( IL 2 ) was evaluated by the CTLL 2 bioassay . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The cellular measures were the absolute numbers of CD 2 , CD 3 , CD 22 , CD 25 , CD 4 , CD 8 and CD4 / CD8 T cell ratio and serologic measures were the levels of cellular products ie interleukin 2 ( IL 2 ) and soluble interleukin 2 receptors ( SIL 2 R ) . ^^^ In addition , a decrease in CD 4 T cells with a relative increase in CD 8 and CD 25 cell count was observed in these groups of patients , leading to a considerable reduction in CD4 / CD8 T cell ratio . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Soluble CD 23 and other receptors ( CD 4 , CD 8 , CD 25 , CD 71 ) in serum of patients with chronic lymphocytic leukemia . ^^^ We measured the soluble ( s ) receptors CD 23 , CD 8 , CD 4 , interleukin 2 receptor ( IL 2R , CD 25 ) , and transferrin receptor ( TfR , CD 71 ) , in normal serum and in patients with chronic lymphocytic leukemia ( CLL ) and evaluated them in relation to clinical and biological parameters of the disease , as well as serum immunoglobulin E ( IgE ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
There were no differences in the expression of CD 3 , CD 4 , CD 8 , CD 25 , CD45RA , CD45RO , LFA 1 , VLA 4 or VLA 6 in inhibited cultures , except for slight decreases in CD 25 and CD45RO in TT cultures . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Monoclonal antibodies against activation antigens , including CD 4 , CD 5 , CD 25 ( interleukin 2 receptor ) , CD 52 , and adhesion molecules , have been investigated in animal studies and open trials in humans . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Transfectants expressing CD 4 , CD 5 , CD 8 , CD 25 , CD 44 , two different WC 1 gene products and a transfectant expressing an unknown gene product were isolated using these techniques . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In HIV 1 seropositive patients a variable number of CD 4 and CD 8 lymphocytes failed to further increase CD 25 , and died as a sign of activation associated lymphocyte death ( AALD ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
They were stained immunohistochemically with a panel of monoclonal antibodies against : T cell subsets ( CD 3 , CD 4 , CD 8 , CD 57 ) , and interleukin 2 receptor ( CD 25 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Suramin also decreased the expression of T cell surface molecules such as CD 2 , CD 25 and CD 4 in preactivated PBMC and had pronounced effects on cytokine production . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The tumor cells represented outgrowth of the original ATL leukemic clone in that they had monoclonal or oligoclonal integrations of the HTLV 1 provirus identical to the leukemic clone and predominantly expressed the cell surface markers , CD 4 and CD 25 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The number , distribution and variation of interleukin 2 receptor ( IL 2R , CD 25 ) and CD 4 positive cells in the above lesions were studied . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The neoplastic cells expressed the T cell receptor ( TCR ) delta chain detected by TCR delta 1 and delta TCS 1 , CD 3 , CD 30 , CD45RO , and epithelial membrane antigen , but not the TCR beta chain detected by beta F 1 , CD1a , CD 2 , CD 4 , CD 5 , CD 7 , CD 8 , CD 25 , HLA DR , and terminal deoxynucleotidyl transferase . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The surface phenotype showed that both the original leukemic cells and the WHN 2 cells had a common phenotype of ATL , i . e . , positive for CD 2 , CD 4 , human leukocyte antigen DR ( HLA DR ) and CD 25 , but negative for CD 8 , a characteristic of helper / inducer T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Flow cytometric analysis revealed a predominance of T cells , especially CD 4 subsets , and higher positivity for both CD 25 and HLA DR . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
UVB had no effect on CD 4 and CD 8 expression or their ratio , whereas the expression of IL 2R ( CD 25 ) was only slightly reduced . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We have studied the expression of several leukocyte surface markers ( CD 2 , CD 3 , CD 4 , CD 8 , CD 7 , CD 26 , CD 25 , CD 14 , CD 18 , kCD 20 , CD 43 , CD 45 , CD45R , CD 71 and HLA class 1 and 2 ) and we find no differences in their expression between PPL . 1 and its parental Jurkat cell line . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Cell surface marker studies for CD 2 ( total T cells ) , CD 4 ( helper T cells ) , CD 8 ( suppressor T cells ) , CD 25 ( activated T cells ) , CD 20 ( total B cells ) , CD 14 ( monocytes ) , and HLA DR positive cell populations were measured . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Induction of gene ( mRNA ) expression of CD 23 , CD 25 and CD 4 on eosinophils by eosinophilopoietic growth lymphokines ] . ^^^ The effects of IL 3 , GM CSF and IL 5 on the expression of CD 23 ( Fc ( E ) RII ) , CD 25 ( IL 2R / p55 ) and CD 4 on an eosinophilic cell line ( EoL 3 ) were investigated by flow cytometry . ^^^ A separate incubation with IL 3 , GM CSF or IL 5 , did not induce the expression of CD 23 , CD 25 , or CD 4 . ^^^ After a further incubation with IL 3 , GM CSF and IL 5 , CD 4 was then expressed , while CD 23 and CD 25 expression still increased . ^^^ Northern blot analysis revealed the presence of mRNA encoding CD 23 , CD 25 and CD 4 in EoL 3 stimulated by IL 3 , GM CSF and IL 5 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Circulating blasts were positive for CD 4 , CD 7 , CD 25 , CD 13 , CD 33 , CD 34 , and HLA DR and partly positive for CD 41 and CD 36 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 and CD 25 double positive cell ( CD4+ / CD25+ ) was 0 . 7 + / 0 . 5 and 1 . 2 + / 0 . 9 % and CD8+ / CD25+ cell was 0 . 4 + / 0 . 4 and 0 . 6 + / 0 . 5 % before and after treatment respectively . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Monoclonal antibodies ( mAb ) to CD11a , CD 25 , and CD 4 were used for three color flow cytometry . ^^^ These results indicate that expression of ICAM 1 in pocket epithelium is relevant to the migration of CD11a , CD 25 , CD 4 positive cells in connective tissue subjacent to the pocket epithelium into the periodontal pocket . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Both CD4+ CD8+ subsets resembled CD4+ CD 8 cells in CD45RA , CD45RO , and CD 25 expression ; the comparable CD CD8+ cells in CD 62 L expression ; and CD 4 CD8bright cells in CD11b , CD11b , CD16 / 56 , and CD 28 expression . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Fresh colonic mucosal biopsy specimens from patients with UC and controls were fixed for the immunohistochemical study of CD 4 , HLA DR , and CD 25 , and other specimens were prepared for the RNA analysis of cytokines . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Aliquots were stained with antibodies to CD 19 ( B cells ) , CD 3 ( T cells ) , CD 4 ( helper ) , CD 8 ( suppressor ) , and the activation marker CD 25 ( IL 2 receptor ) on T cell subsets and were assayed by flow cytometry . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Although the cell separation procedure had no effect on interleukin 2 receptor ( IL 2R , CD 25 ) expression , it induced production of IL 4 by both T cell subsets selected positively , implying cell activation by ligation of CD 4 and CD 8 molecules . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
These findings indicate that levels of sCD 4 and sCD 25 in the CSF are probably associated with meningeal infiltration by leukemia cells expressing CD 4 and CD 25 on surface membranes . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Cardiac allografts in CD 4 mAb treated rats at 24 hours displayed reduced CD 3 , CD 25 , tumor necrosis factor alpha , interleukin ( IL ) 1beta , IL 2 , interferon ( IFN ) gamma , and IL 10 mRNA levels as compared to those in rejecting grafts . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In human peripheral blood lymphocytes ( PBL ) and mouse thymocytes we incorporated light scatter properties , cell cycle stage , relevant cell surface immunophenotypic markers ( CD 25 or CD 4 ) and CD 8 ) and a marker of plasma membrane integrity ( merocyanine 540 ) to enable multiparametric phenotyping of apoptotic cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The effects of the oral administration of 100 mg / kg / day egg white lysozyme ( EWL ) on the expression of CD 3 , CD 4 , CD 8 and CD 25 antigens of lymphocytes harvested from IEL and mesenteric lymph nodes ( MLNL ) were tested in mice bearing MCa mammary carcinoma . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The collective immunoreactive expression of six markers ( S 100 protein , CD 1 a , HLA DR , CD 4 , CD 8 and CD 25 ) was analyzed in 13 cutaneous malignant melanomas . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Results : The absolute counts of CD 4 T lymphocytes expressing the activation marker CD 25 in the peripheral blood on day 8 correlated significantly with the values of the coefficient of variation ( CV ) of both morning PEF ( p < 0 . 01 ) and night PEF ( p < 0 . 05 ) obtained over 7 days , but those of CD8+ / CD25+ T lymphocytes , those of CD23+ B lymphocytes , and the serum concentrations of soluble CD 25 did not . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In ontogeny , F3Ag expression begins in the CD 4 ( ) CD 8 ( ) double negative ( DN ) CD 25 ( + ) population and is maintained through approximately 85 % of the CD 4 ( + ) CD 8 ( + ) double positive ( DP ) stage . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Addition of EtxB to such cultures led to the complete depletion of CD 8 ( + ) T cells compared with enhanced activation of CD 4 ( + ) cells [ as measured by expression of CD 25 ( IL 2Ralpha ) ] . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Since 5 delta 2 responsiveness to mycobacterial Ags was shown to be normally dependent on IL 2 secretion by Th 1 type CD 4 T cells , the ability of IL 2 to restore 5 delta 2 T cells ' responsiveness to TUBAg 1 was tested . 5 delta 2 T cell anergy persisted in spite of the presence of IL 2 , and was frequently correlated with a defect in CD 25 expression on stimulated 5 delta 2 T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Assessment of these T cells for expression of a range of surface molecules after completion of the isolation procedure revealed almost complete loss of expression of CD 4 and CD 25 and reduced expression of a number of other surface antigens . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
A total of 387 HD cases were tested for differences among LMP 1 positive and negative cases with respect to age , sex , histotype and immunophenotypic parameters ( CD 2 , CD 3 , CD 4 , CD 15 , CD 19 , CD 20 , CD 21 , CD 22 , CD 23 , CD 25 , CD 30 , CD 43 , CD45RA , CD45R0 , CD 70 , HLA DR , T cell receptor beta chain , and p 53 expression ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
These abnormal lymphocytes ( leukemic cells ) were positive for CD 4 , and some expressed IL 2R ( CD 25 ) and CD 3 molecules , respectively . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The percentage of T cells expressing CD 25 and CD 26 varied no more than repeated estimation of the CD 4 T cell subset , whereas other actags showed greater variability . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Immunohistochemical staining was performed on cryostat sections with monoclonal antibodies against : eosinophil cationic protein ( EG 1 , EG 2 ) , mast cell tryptase ( AA 1 ) , CD 45 , CD 22 , CD 3 , CD 4 , CD 8 , CD 25 , and CD45RO . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Expression of surface CD 3 , CD 4 and CD 8 and the activation marker CD 25 was quantified on T lymphocytes using flow cytometry . ^^^ In contrast , CD 25 expression on both CD 4 and CD 8 T lymphocytes was inhibited by both drugs to an equivalent extent over the same range of concentrations . ^^^ At this time point , the degree of inhibition of CD 4 re expression by both drugs correlated significantly with the degree of inhibition of CD 25 expression . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
A large population of YAP cells reacted with the CD 4 , CD11b , CD 25 and CD 33 surface markers , but not with CD 2 , CD 3 , CD 8 or CD 19 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
On the other hand , CD 4 ( ) CD 8 ( ) double negative ( DN ) thymocytes , most of which were arrested at the CD 44 ( ) CD 25 ( + ) stage similarly to those in zeta deficient mice , could not be promoted for differentiation into CD 4 ( + ) CD 8 ( + ) double positive thymocytes in these mice in spite of the fact that the expression of the transgene in DN thymocytes was higher than that of zeta in wild type mice . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The CD 3 , CD 4 , CD 8 triple negative thymic subset was composed almost entirely of the earliest population ( CD44+ , CD 25 ) , with the remaining maturational stages ( CD44+ , CD25+ ; CD 44 , CD25+ ; and CD 44 , CD 25 ) depleted . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
To further examine the immune mechanisms which potentially are involved in alcoholic cirrhosis and the relationship to ethanol , we have determined the expression of surface antigens CD 4 , CD 8 , and of adhesion molecules CD 25 , LFA 1 , ICAM 1 and LFA 3 in patients and in response to stimulation with OKT 3 , IL 2 and with ethanol in vitro . ^^^ Lymphocytes from patients showed increased basal and stimulated expression of CD 4 , CD 25 , LFA 1 , ICAM 1 and LFA 3 molecules and increased TNF alpha production in comparison to controls . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
RESULTS : There was a significant ( p < 0 . 01 ) difference in the proportion of CD 4 positive cells with CD 25 between BD patients with active uveoretinitis ( 27 . 6 % ( SD 8 . 4 % ) ) and the controls ( 14 . 7 % ( 2 . 3 % ) ) , but no significant difference in the proportion of CD 4 or CD45RO positive cells with Fas . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Specimens were analysed histologically and by indirect immunoperoxidase method using anti monoclonal antibodies to CD 2 , CD 4 , CD 8 , CD 25 , CD45RO , RFDR 1 , RFD 1 and RFD 7 by two ' blind ' observers . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 25 and class 2 MHC molecule expression and the CD 4 : CD 8 ratio remained unchanged over the comparable period in the syngeneic LEW to LEW control model ( n = 5 , p > 0 . 3 for all comparisons ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
He was diagnosed as ATL by leukocytosis [ leukocyte count 18 , 200 / microliters with 56 % of abnormal lymphocytes which express CD 4 ( + ) and CD 25 ( + ) ] and seropositive result of anti HTLV 1 antibody . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
On PHA activated , Fas sensitive T cell blasts , anti Fas mAb rapidly triggered downmodulation of a variety of antigens , including CD 2 , CD 4 , CD 8 , CD 7 , CD 44 , LFA1alpha , LFA1beta , and CD62L , but not CD 3 , CD 25 , or CD 26 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Some of these TCR antagonistic analogues carrying relatively conservative amino acid substitutions partially activated the T cells to induce large increases in size and expression levels of CD 4 , CD11a ( LFA 1 ) , CD 28 , CD49d ( VLA 4 ) , and CD 95 ( Fas ) , and small increases in CD 25 and CD 44 expressions on the cell surface . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Groups of mice from both strains were killed at 3 , 5 , 10 , 15 , 30 , and 150 days after infection and lymphocytes were harvested from the peritoneal exudate cells ( PEC ) , spleen , intestinal epithelial lymphocytes ( IEL ) , lamina propria lymphocytes ( LPL ) , Peyer ' s patches , and mesenteric lymph node ( MLN ) and labelled with monoclonal antibodies to CD 3 , CD 4 , CD 8 , gamma delta TCR , CD 25 , and CD 44 for flow cytometric analysis . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
However , CD 4 mAbs decreased neither the number of blast cells nor the expression of CD 25 ( the alpha chain of IL 2 receptor ) , indicating that initial activation events leading to blast transformation were not affected . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The polypectomy specimens taken from the rectum revealed infiltration of neoplastic T cells , the integration of HTLV 1 proviral DNA , and increased CD 4 ( OKT 4 ) and CD 25 ( IL 2R ) cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The mononuclear cells stained positively for Pan T , CD 2 , CD 3 , CD 4 , CD 5 , CD 25 , and DNA from the cells showed HTLV 1 provirus monoclonal integration . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Frozen sections were stained with monoclonal antibodies directed against IL 7R , CD 25 , CD 30 and T antigens ( CD 3 , CD 2 , CD 5 , CD 7 , CD 4 , CD 8 ) , using the alkaline phosphatase antialkaline phosphatase technique . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Percentages of the different lymphocytes subsets , including CD 2 ( T cells ) , CD 4 , CD 45 RA ( suppressor inducer / virgin T cells ) , CD 4 , CD 29 , CD 8 , CD 19 ( B cells ) , as well as interleukin 2 ( IL 2 ) receptor ( CD 25 ) and class 2 antigen ( human leukocyte antigen HLA DR ) expression , were analyzed by flow cytometry . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Frozen sections of eyelids and conjunctiva were immunostained with the following monoclonal antibody markers : W3 / 13 ( CD 3 ) , W3 / 25 ( CD 4 , macrophages ) , OX 8 ( CD 8 ) , MARD 3 ( B cells ) , ED 1 , ED 2 ( macro / monocytes ) , OX 6 ( class 2 MHC , Ia ) and OX 39 ( CD 25 , IL 2 receptor ) . ^^^ The p values were < 0 . 02 for W3 / 13 ( CD 3 ) , W3 / 25 ( CD 4 ) , OX 8 ( CD 8 ) , OX 39 ( CD 25 ) ( IE only ) , ED 1 , ED 2 and OX 6 positive cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Inhibitory lysates did not significantly change CD 25 expression on either CD 4 , CD 8 , or CD45R0 lymphocytes by flow cytometry . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Moreover , in both strains , blood CD 4 , CD 8 , CD 25 , naive CD 4 ( CD4 / CD45RC ) cell and B ( CD45R ) cell counts and plasma corticosterone levels , constituents of systemic inflammatory responses to carrageenan were evaluated . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
On this basis , we have undertaken a study in order to examine , in addition to activation markers such as CD 25 and CD 54 ( ICAM 1 ) on peripheral blood mononuclear cells ( PBMNCs ) surface , serum levels of soluble interleukin ( IL ) 2 receptor ( sIL 2R ) , soluble ICAM 1 ( sICAM 1 ) , soluble CD 4 ( sCD 4 ) , soluble CD 8 ( sCD 8 ) , beta 2 microglobulin and fibronectin ( FN ) in psoriatic patients analyzed both in acute and remission phase obtained by topical therapy alone . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
To evaluate whether these effects were related to the modulation of T lymphocyte recruitment and activation and cytokine expression , we performed immunohistochemical staining for T cell surface marker CD 3 and CD 4 , cell activation marker CD 25 , and cytokines granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 4 ( IL 4 ) and interleukin 5 ( IL 5 ) on lung tissue sections from mice exposed to Aspergillus fumigatus and treated or not with dexamethasone or cyclosporin A . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Cytopreparations of BAL fluid ( n = 13 ) and cryostat sections ( five mediastinal nodes , 14 transbronchial biopsies ) were stained with alkaline phosphatase antialkaline phosphatase and monoclonal antibodies to CD 3 , CD 4 , CD 8 , CD 25 , and gamma delta T cell receptor ( TCR ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The CD11b / CD18 expression was related to the magnitude of DCFH oxidation , but not to lymphocyte numbers or subpopulations ( CD 4 , CD 8 , CD 25 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In addition , the phenotype of the cells was measured using flow cytometry with antibodies against CD 4 , CD 8 , CD 5 , MHC class 2 , CD 25 , CD 14 and CD 19 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Compared to normal controls , both atopic groups showed higher IL 2 and IL 5 production ( p < 0 . 01 and p < 0 . 05 ) and higher percentages of CD 4 cells expressing CD 25 and HLA DR ( p < 0 . 05 ) in response to rye grass pollen , elevated IL 4 production in response to PHA ( p < 0 . 01 ) , and a non significant trend towards lower IFN gamma production . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Surface molecules of CTL lines were analysed by flow cytometry using MAbs specific for CD 4 , CD 8 , CD 16 , CD 25 , CD 56 , HLA DR , T cell antigen receptor ( TCR ) alpha beta and TCR gamma delta . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
A significant proportion of LP CD 4 T cells are CD 25 ( IL 2R ) positive , but following activation they secrete IL 4 , with little or no IL 2 production . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Tests for the peripheral blood mononuclear cell phenotype ( CD 3 , CD 4 , CD 8 , CD 57 [ corrected ] , CD 20 , and CD 25 ) , spontaneous proliferation , and the proliferative response to phytohemagglutinin , pokeweed mitogen , and the lipopolysaccharide of S . dysenteriae 1 were performed , as were skin tests for delayed type hypersensitivity ( DTH ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
B220+ fetal liver cells did not contain detectable D J rearrangement of TCR beta gene and were c kit+ IL 7R+ Thy 1 CD 3 CD 4 ( low ) CD 8 CD 25 CD44+ . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The test laboratory examination was performed in all patients : complete blood cell count , immunoglobulins A , G , M and E determination , lymphocyte subpopulations CD 3 , CD 4 , CD 8 , CD 4 CD8 proportion , CD 25 , rosette formation for B and T lymphocytes , coproparasitoscopic examination , throat and nose cultures , nasal cytology , skin tests of cellular immunity to PPD , thrichophytin , candidine , varidasa ; skin prick test to poliens , fungi , inhalants and foods . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The main findings were that impaired proliferative responses to mitogens correlated with ( 1 ) decreased cell surface expression of the interleukin 2 receptor ( CD 25 ) , ( 2 ) increased expression of HLA DR antigens on CD 4 cells , ( 3 ) reduced CD 4 and increased CD 8 cell numbers , and ( 4 ) increased levels of serum immune complex dissociated p 24 antigen . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Adult T cell leukemia / lymphoma ( ATL ) was detected in 4 patients including leukemia with proliferation of CD 4 and CD 25 in two cases and lymphoma in one case . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
These CD 4 cells were functional , demonstrating normal proliferative responses and up regulation of CD 25 and CD 69 after activation by mitogens or alloantigens . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The skin biopsies were stained to detect T cell subsets ( CD 4 , CD 8 , CD45Ro and CD 25 ) , macrophage subsets ( RFD 7 ) , dendritic cells ( RFD 1 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Unlike the lymphocyte counts and activities of patients from the control group who gave preoperative values after 3 6 months ) , mistletoe treatment induced a statistically significant up regulation of cell counts ( CD 3 , CD 4 , CD 8 cells ) and activities ( CD 25 , HLA / DR positive cells ) after 3 months , as compared to preoperative values . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The cell surface markers included complement receptors [ CR 1 ( CD 35 ) , CR 2 ( CD 21 ) , CR 3 ( CD11b , CD 18 ) ] , immunoglobulin Fc receptors [ FcgammaRII ( CD 32 ) , FcgammaRIII ( CD 16 ) ] , proteins important for lymphocyte function [ CD 3 , CD 4 , CD 8 , CD 19 , CD 25 , CD45RA ] , and the framework antigen HLA ABC . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Quantitative alterations of the functionally distinct subsets of CD 4 and CD 8 T lymphocytes in asymptomatic HIV infection : changes in the expression of CD45RO , CD45RA , CD11b , CD 38 , HLA DR , and CD 25 antigens . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Lymphocytes were analyzed for expression of CD 2 , CD 3 , CD 4 , CD 8 , CD 25 , gamma delta TCR , and CD 20 by two or three color flow cytometric analysis . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In 15 subjects , criostal sections were immunostained with anti CD 3 , CD 4 , CD 8 and CD 25 antibodies . ^^^ There was a significant positive correlation between the numbers of Hansel staining positive cells and CD 3 , CD 4 and CD 25 positive cells , and between the numbers of EG 2 positive cells and CD 4 , CD 25 positive cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Cells were analyzed from the arterial line before , at 5 , 10 , 15 , 30 , 90 and 240 min with a panel of 33 surface markers including antibodies towards CD 3 , CD 4 , TCR alpha / beta , CD 7 , CD 8 , CD11a c , CD 14 , CD 18 , CD 19 , CD 25 , CD 28 , CD 29 , CD 38 , CD 44 , CD49a e , CD51 / 61 , CD 54 , CD 56 , CD 58 , CD 62 , CD 106 , and HLA class 2 using single fluorescence analysis . ^^^ Our results suggest a substantial difference in the expression of various surface markers and cell adhesion molecules ( lymphocytes : CD 4 , CD 25 , CD 28 , CD 51 , CD 54 ; monocytes : CD 49 , CD 54 , CD 58 , CD62E , CD62P ) compared to healthy persons , and a distinct modulation during hemodialysis ( lymphocytes : CD 11 , CD 18 , CD 49 , CD 51 , CD 61 ; monocytes : CD 29 , CD 49 , CD 51 , CD 54 , CD 61 ) which might affect the immune response beyond the single dialysis session . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
IL 8 concentrations were measured by an ELISA method while immunophenotypic analysis was performed by using FITC conjugated monoclonal antibodies reacting against the following cell surface molecules : CD 3 , CD 4 , CD 8 , CD 25 ( IL 2R+ cells ) , CD 19 , and CD 68 . ^^^ Unlike the pleural compartment , patients with TBP showed a marked and significant decrease in their circulating levels of cells bearing the CD 3 , CD 4 , CD 19 , CD 25 , and CD 68 phenotypes not only when comparing with Co but also with PNE and mild patients . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
While displaying normal development of CD 2 , CD 3 , CD 4 , and CD 8 expression , CD 25 deficient cortical thymocytes do not express CD 1 , and furthermore they fail to normally down regulate levels of the anti apoptotic protein bcl 2 . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
METHODS : Conjunctival biopsy specimens obtained 48 hours after a 2 mm biopsy of the epibulbar conjunctiva in 26 Turkish patients with inactive ocular Behet disease and 9 Turkish patients with inactive idiopathic uveitis were studied by immunoperoxidase using a panel of monoclonal antibodies : anti CD 1 , CD 3 , CD 4 , CD 5 , CD 14 , CD 22 , CD 25 , and CD 67 , HLA DR , E selectin , intercellular adhesion molecule 1 ( ICAM 1 ) , and vascular cell adhesion molecule 1 ( VCAM 1 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Frozen sections of small bowel were studied with monoclonal antibodies anti CD 5 , CD 4 , CD 8 , CD 25 , macrophages , MHC 2 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
RESULTS : The more severe ( Group 1 ) as compared with the milder ( Group 2 ) asthmatics showed evidence of increased peripheral blood T cell activation , with elevated percentages of CD 4 cells expressing the activation markers CD 25 and HLA DR , and CD 8 cells expressing CD 25 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The CD 3 CD 4 CD 8 subset showed a significant decline in number by 20 mo of age , but despite this reduction , no significant difference was noted in the number of CD44+ CD 25 cells , the earliest stage of this subset between 3 and 20 mo of age . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Most primitive thymic progenitors , termed CD 4 ( low ) cells ( CD 25 CD44+ CD117+ ) , retain the ability to generate multiple lymphoid lineages . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The results indicated that following stimulation with IPP , gamma delta T cells express CD 25 and CD 69 antigens , but fail to proliferate unless growth factors are provided exogenously or endogenously through activation of CD 4 T cells by additional stimuli such as tetanus toxoid , alloantigen , or superantigens . ^^^ In response to IPP stimulation , purified CD 4 T cells neither express CD 25 or CD 69 , nor do they proliferate even in the presence of exogenous IL 2 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In both cases a lymphocyte transformation test ( LTT ) was performed and we analyzed the T cell activation parameters CD 25 and HLA DR on CD 4 and CD 8 T cells to demonstrate in vivo activation of T cells during the active disease . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The ration of CD 3 , CD 4 , CD 8 , CD 19 , CD 5 , CD 25 HLA DR cells in full blood was assessed by the monoclonal antibody technique of direct immunofluorescence , using evaluation by means of a flow cytometer . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Flow cytometric analysis showed that T cells from toxin treated mice had lower membrane CD 3 , CD 4 and CD 8 density , and higher percentages of CD8+ splenocytes and CD4+ thymocytes expressing CD 25 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Interferons alpha / beta inhibit IL 7 induced proliferation of CD 4 CD 8 CD 3 CD44+ CD25+ thymocytes , but do not inhibit that of CD 4 CD 8 CD 3 CD 44 CD 25 thymocytes . ^^^ The IL 7 induced proliferation of CD 4 CD 8 thymocytes from T cell receptor ( TCR ) transgenic mice , the majority of which are CD3+ CD 44 CD 25 , was resistant to IFN alpha / beta mediated suppression . ^^^ In contrast , the addition of IL 7 markedly decreased the percentages of CD 4 CD 8 CD 3 CD 44 CD 25 cells , and IFN alpha / beta reversed the effect and increased the subpopulations of CD 44 CD25+ and CD 44 CD 25 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The early thymocyte developmental stages , based on CD 44 and CD 25 expression , revealed the CD 4 CD8 CD 3 triple negative compartment ( TN ) to be composed of almost entirely the earliest population ( CD44+CD25 ) with the remaining maturational stages depleted . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Peripheral blood ( MOAb for immunophenotyping of lymphocytes : CD 14 , CD 45 , CD 3 , CD 19 , CD 4 , CD 8 , CD16 / 56 , HLA DR , CD 38 , CD 25 ) is the material of choice for detection and monitoring of immunodeficiences . ^^^ Extravascular fluids , suspension of single cells and BAL are preferred materials for detection and classification of non Hodgkin lymphomas ( MOAb for immunophenotyping of lymphocytes : CD 14 , CD 45 , CD 3 , CD 19 , CD 4 , CD 8 , CD16 / 56 , HLA DR , CD 38 , CD 25 , CD 23 , CD 5 , CDl1c , CD 30 , light chain immunoglobulins ) . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Expression of CD 3 , CD 4 , CD 8 , CD 20 , HLA DR , and CD 25 molecules in lymphocytic and epithelial cells on minor salivary glands was detected by immunohistochemical assays . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Despite being CD69+ , CD 4 cells did not express the high affinity IL 2 receptor chain , CD 25 , to any significant extent on any of the days studied . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
PMA inhibited cell growth and decreased the amounts of CD1a and CD 4 while CD 3 , CD 8 , CD 25 , CD 45 , CD 57 and MHCI increased it ; the p 53 anti oncogene increased while actin levels remained constant . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Cellular infiltration was studied using immunohistochemistry with monoclonal antibodies using CD 3 , CD 4 , CD 8 , CD 25 , major basic protein ( BMK 13 ) , eosinophil cationic protein ( EG 2 ) , neutrophil elastase , and tryptase . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Coexpression of CD 4 and CD 8 antigens and the appearance of HLA DR and CD 25 receptors on lymphocytes as markers of immune activation in patients with HIV infection ] . ^^^ Results of quantitative determinations of peripheral blood lymphocytes presenting CD 4 or simultaneously CD 4 and CD 8 antigens as well as HLA DR and CD 25 antigens in patients with different clinical stages of CD4+ and CD4+CD8+ cells were observed . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
METHOD : The cornea and adjacent bulbar conjunctiva taken from 14 patients with Mooren ' s ulcer were stained immunohistochemically for CD 3 , CD 4 , CD 8 , HLA DR , CD 1 and CD 25 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The percentage of cells positive for CD 19 , CD 25 , CD 38 , and CD 71 was significantly lower during convalescence compared with pre IVIG ( medians : CD 19 , 18 % vs 26 % , P = 0 . 0004 ; CD 25 , 6 % vs 9 % for CD 3 ( + ) cells , P = 0 . 0074 ; CD 38 , 78 % vs 89 % for CD 8 ( + ) cells , P = 0 . 0015 ; CD 71 , 1 % vs 6 % for CD 4 ( + ) cells , P = 0 . 0024 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Specimens were stained immunohistochemically with monoclonal antibodies against stored eosinophil cationic protein ( EG 1 ) , the secreted form of eosinophil cationic protein ( EG 2 ) , mast cell tryptase ( AA 1 ) , neutrophil elastase ( NP 57 ) , CD 3 , CD 4 , CD 8 , and CD 25 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Immunohistopathological studies were performed on cryostat sections of bucal mucosa biopsies using antibodies to CD 3 , CD 4 , CD 8 , CD 20 , CD 14 , CD 25 , LFA 1 , ICAM 3 HLA class 2 , tumor necrosis factor alpha , interleukin 1 , and interferon gamma . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
There were no significant differences in the total and differential leukocyte counts as well as the lymphocyte subpopulations : CD 3 ( T cells ) , CD 19 ( B cells ) , CD 4 ( T helper cells ) , CD 8 ( T suppression cells ) , CD4 / CD8 ratio , and CD 25 ( Interleukin 2 receptor cells ) between the two subject groups throughout the experimental period . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
This was accompanied by a reduction of EG 2 ( + ) eosinophils ( p < 0 . 01 ) , CD 3 ( + ) ( p < 0 . 001 ) , CD 4 ( + ) ( p < 0 . 05 ) , CD 8 ( + ) ( p < 0 . 05 ) T lymphocytes , and CD 25 ( + ) activated T lymphocytes ( p < 0 . 001 ) in the bronchial mucosa . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Specimens were snap frozen and immunohistochemical staining was performed on cryostat sections with monoclonal antibodies against : ( secreted ) eosinophil cationic protein ( EG 1 , EG 2 ) , mast cell tryptase ( AA 1 ) , CD 45 , CD 22 , CD 4 , CD 8 , CD 25 , and CD45RO . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We used a flow cytometry technique , the `` FOG ' ' method ( formaldehyde and octylglucopyranoside ) , to investigate the expression of activation antigens , i . e . , CD 4 , CD 23 , CD 25 , HLA DR , and the EG 2 epitope of eosinophilic cationic protein , on peripheral blood eosinophils ( PBEs ) in leukemic patients who had developed acute graft vs . host disease ( aGVHD ) with eosinophilia after allogeneic bone marrow transplantation ( alloBMT ) or leukocyte buffy coat infusion . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
All glycosylphospha tidylinositol ( GPI ) anchored proteins ( CD 14 , CD16B , CD 48 , CD 59 , CD 87 and GPI anchored versions of CD 2 and CD 25 ) and protein tyrosine kinase ( PTK ) Lck but not transmembrane proteins ( CD 2 , CD 4 , CD 5 , CD 6 , CD 8 ) and PTK ZAP 70 were in part localized in buoyant , lipid rich , detergent resistant membrane GPI microdomains of the COS cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
To characterize immune responses to these immunogens , we examined the production of antibodies to the B 700 melanoma antigen , the stimulation of endogenous IL 2 production , the expression of CD 4 , CD 8 , Vbeta and CD 25 T cell markers , and the induction of NK activity . ^^^ Levels of antibodies to the B 700 melanoma antigen were also significantly higher in mice immunized with the SEA secreting B 16 cells , as was expression of CD 4 , CD 8 , CD 25 and Vbeta T cell antigens , particularly CD 4 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
While the conventional view holds that LPS is ignored by T cells , we report here that administration of LPS to mice activates all B cells , but also engages most CD 4 and CD 8 T cells , as measured by the expression of the activation markers CD 69 and CD 25 and by size increase . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Intravenous immunoglobulins , cytokines , and anticytokines , immunotoxins , monoclonal anti leukocyte antibodies ( CD 4 , CD 25 ) and soon peptide vaccination constitute the new types of treatments . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Both MS Tg mice and CD 80 Tg mice by themselves showed no evidence for activation of T cells and B cells , whereas MS 10 CD 80 double Tg mice with a H 2k background revealed an abnormal increase in the number of splenocytes and in the expression of activation markers ( CD 69 and CD 25 ) on CD 4 T cells in the spleen . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Expression of the early activation markers IL 2R alpha chain ( CD 25 ) and CD 69 on splenic donor CD 4 ( + ) cells was considerably higher at early time points ( 36 and 72 hours post BMT ) in IL 12 treated mice compared with GVHD controls . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The cell binding carriers of anti HIV ITs have been directed against different regions of the HIV envelope glycoprotein ( gp 120 and gp 41 ) and surface antigens ( e . g CD 4 , CD 25 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
At remission , the number of CD 4 , CD 25 , and CD45RO positive cells decreased , while CD 8 and CD45RA positive cells increased to normal levels as previously reported by Suzuki et al . [ 1 ] . ^^^ FCM using mononuclear cells in the fluid revealed that 87 . 3 % of the cells were double positive for CD 4 and CD 25 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
A humanised therapeutic CD 4 mAb inhibits TCR induced IL 2 , IL 4 , and IL 10 secretion and expression of CD 25 , CD40L , and CD 69 . ^^^ The CD 4 mAb inhibited the CD 3 induced expression of the CD 25 and CD 69 activation markers on the T cell surface and suppressed CD 40 ligand expression , but not that of CD 25 and CD 69 , when their expression was induced by phorbol ester plus ionomycin . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Intracytoplasmic cytokine staining of PBMC combined with surface marker detection revealed that IL 10 was produced initially by activated CD 4 ( + ) CD 25 ( + ) , allergen specific T cells , and followed by B cells and monocytes . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Immunohistochemistry was performed using monoclonal antibodies ( MAb ) specific for CD 3 , CD 4 , CD 8 , CD 22 , CD 25 , CD 38 , CD 68 , MAb 67 , Ki 67 , interferon gamma ( IFN gamma ) , interleukin ( IL ) 1alpha , IL 1beta , tumour necrosis factor alpha ( TNF alpha ) , E selectin , ICAM 1 and VCAM 1 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Quantitative immunohistochemistry analyzing a panel of immune cell types , including T lymphocytes , T helper / inducer cells ( CD 4 ) , T suppressor / cytotoxic cells ( CD 8 ) , T cells positive for interleukin 2 receptor ( CD 25 ) , B lymphocytes and plasma cells , macrophages , natural killer cells , granulocytes and all leukocytes expressing the leukocyte common antigen ( CD 45 ) , yielded no evidence for the ability of VAA to stimulate a substantial cellular immunological reaction in the wall of the normal urinary bladder or during urothelial carcinogenesis . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Expression of CD 4 and CD 25 and sensitivity to MTX returned to near parental levels after removal of MTX from culture medium , whereas expression of CD 26 and MTX uptake were significantly increased . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Cell surface markers CD 3 , CD 4 ( OKT 4 and Leu 3a ) , CD 8 , CD 20 , CD 25 , CD45Ro , CD45Ra and DR were analyzed , and proliferation to mitogens and recall antigens was measured . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The statistical analysis of results showed that two sets of variables may be distinguished : ( a ) the first set , including CD 20 , CD 4 , CD 8 cell markers and CD20 / CD3 and CD4 / CD8 ratios , that was able to differentiate between FA patients and the other subject groups ; ( b ) the second set , including CD 25 , HLA DR , HLA DP , HLA DQ cell markers , that was able to differentiate healthy subjects from the other groups . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In a dose escalation study , we found that this tumor immunogen induced a moderate local inflammatory response consisting predominantly of CD 4 ( + ) T lymphocytes , and a systemic response , with a rise in circulating CD 25 ( + ) and DR+ CD 3 ( + ) T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The monoclonal antibody panel included RT 1 ( a ) ( ACI , MHC 1 ) combined with CD 2 , CD 4 , CD 8 , CD 16 , and CD 25 or RT 1 ( a , c ) ( bone marrow chimeras ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Thy1 . 2 ( CDw 90 ) , 1 A ( MHC class 2 . glycoprotein ) , L3T4 ( CD 4 ) , IL 2R ( CD 25 ) and CD 3 , is considerably increased in Con A stimulated T lymphocytes . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Both cortical TEC subsets are also present in recombination activating gene 1 ( RAG 1 ( / ) ) and TCRbetaxdelta / thymi in which T cell development is blocked at the CD 4 ( ) CD 8 ( ) CD 25 ( + ) CD 44 ( ) pre T cell stage . ^^^ In contrast , K 8 ( + ) K 5 ( + ) TECs predominate in the thymi of human CD3epsilon transgenic mice in which thymocyte development is blocked at an earlier CD 4 ( ) CD 8 ( ) CD 25 ( ) CD 44 ( + ) stage . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
At T 1 and T 2 , eight patients showed an up regulation of CD 25 on CD 4 , as well as an increase in the CD4+CD25+ cell number . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
There was no significant increase at night in the number of CD 3 , CD 4 , CD 8 , CD 25 , AAI ( tryptase ) and EG 2 immunopositive cells in the submucosa in both groups . ^^^ The number of EG 2 , CD 4 and CD 25 positive cells at 04 : 00 and 16 : 00 h tended to be higher in asthmatics with a PEF variation > 15 % than in asthmatics with PEF variation < or =15 % . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Under our experimental conditions , Emdogain produced a slight increase of the proliferation of lymphocytes , restricted to the CD 25 ( IL 2 receptor ) fraction of the CD 4 positive T lymphocytes , and a concomitant decrease of CD 19 positive B lymphocytes . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We studied by simple and double immunolabeling techniques on tissue sections of normal human skin the phenotype of these cells and found them to express vimentin and Te 7 but none of the remaining markers sought ( factor XIIIa , von Willebrand factor , CD1a , CD 3 , CD 4 , CD 8 , CD 14 , CD 25 , CD 36 , CD 45 , CD 54 , CD 56 , LFA 1 , EGF R , S 100 protein , Mac 387 , and muscle specific actin ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
METHODS : IEL and LPL were obtained from 18 CD patients and 20 controls by enzymatic extraction , and subsequently characterized by flow cytometry for CD 3 , CD 4 , CD 8 , CD 25 , LFA 1 alpha ( CD11a ) , CD 44 , alpha 4 and alpha E beta 7 integrin . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 25 was mainly expressed by stimulated CD 4 ( + ) T cells , and its detection on total T cells was both a sensitive ( 98 % ) and a specific ( 97 % ) indicator of prior T . gondii infection . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In macaques , the proportion of CD 4 ( + ) T lymphocytes expressing CD 25 declined during SIV infection , while in mangabeys , CD 25 expressing CD 4 ( + ) T lymphocytes increased . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Examination of immunological parameters revealed a transient decrease in CD 25 ( + ) peripheral blood lymphocytes and , in seven of eight patients , an increase in CD 4 ( + ) : CD 8 ( + ) ratios at 2 weeks . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
T cell subsets and activated T cells were analysed by flow cytometry with dual stain using monoclonal antibodies to CD 3 , CD 4 , CD 8 and CD 25 . ^^^ The ESEA correlated with the percentage of eosinophils ( p < 0 . 05 ) and that of CD 25 ( + ) CD 4 lymphocytes ( p < 0 . 05 ) of BAL cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Lymphocyte subpopulations were examined using flow cytometry and monoclonal antibodies against CD 4 , CD 8 , CD 20 , CD 25 , CD45RA , and HLA DR in 34 patients with non segmental vitiligo . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
A clear circadian rhythm was validated for cortisol serum levels , for CD 8 , CD 8 dim , CD 16 and delta TcS 1 with acrophase in the morning , and for CD 2 , CD 4 , CD / CD8 ratio , HLA DR , CD 20 and CD 25 with acrophase at night . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
METHODS : We investigated in vitro induction of activated memory helper T cells ( CD 4 ( + ) CD 25 ( + ) CD45RO+ cells ) in response to gelatin in children with nonanaphylactic reactions to vaccines containing gelatin . ^^^ RESULTS : In patients with delayed type sensitivity to gelatin confirmed with a positive skin test response , CD 4 ( + ) CD 25 ( + ) CD45RO+ cells were significantly more strongly induced in culture containing gelatin than in control cultures . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
RESULTS : Granulocyte macrophage colony stimulating factor ( GM CSF ) , tumor necrosis factor alpha ( TNF alpha ) and sIL 2R were elevated , and newly expressed antigens on eosinophils CD 4 , CD 25 , and HLA DR were found to be involved in the pathophysiology of this disorder . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The lymphocyte populations ( T , B , NK cells ) , and the activated T cells ( CD 25 , and HLA Dr positive CD 4 and CD 8 cells ) were analyzed by flow cytometry with dual color direct immunofluorescence after whole blood lysis . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The frequency of activated ( CD 25 ( + ) ) and helper ( CD 4 ( + ) ) T cells appeared to be increased in Ag sites as well , though not significantly . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
PATIENTS AND METHODS : We have studied the minor salivary glands in 30 patients with pSS , 30 patients with sSS , 19 patients with AD without SS and 18 controls , using immunohistochemical techniques to analyze the molecular expression of CD 3 , CD 4 , CD 8 , CD 20 , CD 25 , CD 14 , CD 56 , CD11a , CDw 50 ( ICAM 3 ) , HLA DR , IL 1 , TNF alpha and IFN gamma in lymphocytic infiltration and epithelial cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
However , no significant changes in circulating CD 3 , CD 4 , CD 8 , CD 19 , or CD 25 cells were found , evaluated by FACS analysis . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Activated T cells treated with sub lethal levels of BPD MA and light exhibited lower CD 25 levels , a temporary block in cell cycle transition , but unaltered expression of MHC Class 1 , CD 3 , CD 4 , CD 8 , CD 45 , CD 54 , CD 71 , CD 122 ( IL 2R beta chain ) or TCR beta chain antigens 24 h afterward . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Also , the method of direct immunoluminescence using fluoroesceinisothiocymate labeled monoclonal antibodies against human antigens CD 4 , CD 8 , CD 25 and CD 45 was employed . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Otherwise , the prophylactic intracavitary BCG instillations in SBTCC patients are associated with a transitory variation of T lymphocyte subset distribution ( CD 4 and CD 8 ) and activation antigens expression ( CD 25 ) . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
METHODS : The serum concentration of sIL2R , TPA and lymphocyte subsets CD 4 , CD 8 , CD 25 , CD 16 were measured at diagnosis and then 1 and 6 months after the start of chemotherapy . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The CD1a+ cells reacted with antibodies against protein S 100 , HLA DR , the Lag antigen , CD 4 , CD11a , CD11b , CD 18 , CD 25 , CD 40 , CD 54 , CD 80 and CD 86 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In response to two types of measles virus ( MV ) antigens , a vaccine strain CAM and a wild strain isolated in 1994 , the expression of IL 2 receptor alpha ( CD 25 ) ( + ) CD45RO ( + ) CD 4 ( + ) T lymphocytes ( T cell activation ) was analyzed by flow cytometry . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Expression of interleukin 2 receptor , CD 25 , on CD 4 lymphocytes in response to varicella zoster virus antigen among patients with malignancies immunized with live attenuated varicella vaccine . ^^^ CONTROLS : The percentage of CD 25 positive cells among CD 4 lymphocytes significantly increased in response to the VZV antigen in vaccinated patients and patients with past natural varicella infection as observed in the controls . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The CD 4 ( + ) CD 8 ( + ) cells were the major ( > 90 % ) population that expressed elevated levels of CD 25 in BALB / c mice . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Analyses included a flow cytometric immunophenotyping of peripheral blood mononuclear cells ( CD 3 , CD 4 , CD 8 , CD 19 , CD 25 , CD 28 , CD 56 , CD 57 , CD 80 and HLA . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Fluorescence activated cellular cytometry analysis revealed unaltered expression of the TCR / CD3 complex in the anergized clone ; however , both CD 4 and CD 25 expression increased after 24 hr of stimulation by SEA under these conditions . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
METHODS : B and T lymphocyte activation was studied by cytometric assessment of the expression of the activation markers CD 38 on B cells and CD 25 and HLA DR on CD 4 ( + ) and CD 8 ( + ) T cell subsets , respectively . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
RESULTS : We found no differences among the study groups with respect to the general proportions of T cells , CD 4 ( + ) T cells , CD 8 ( + ) T cells , B cells , NK cells , CD 103 ( + ) T cells , and CD 25 ( + ) T cells among total circulating lymphocytes . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Further , a small population of CD 4 ( + ) T cells expressing CTLA 4 , CD 25 and CD45RBlow was identified in mice that had not been treated with specific peptide . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
METHODS : Paired peripheral blood ( PB ) and SF samples were analyzed by 3 color flow cytometry using monoclonal antibodies to CD 3 , CD 4 , CD 8 , HLA DR , CD 25 , CD45RA , and CD45RO . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Lymphocyte measurements included total lymphocyte counts ; T cell ( CD 3 , CD 4 , CD 5 , and CD 8 ) , B cell ( CD 20 ) , and NK cell ( CD 16 and CD 56 ) subsets ; and expression of the activation antigen CD 25 on CD 3 T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In human T cell stimulation studies in vitro , the bsCD 28 vaccine caused an up regulation of early ( CD 69 ) and late ( CD 25 ) T cell activation markers on CD 4 and CD 8 T lymphocytes from either normal healthy donors or cancer patients ( autologous system ) and induced tumor cytostasis in nonmodified bystander tumor cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Also , there is a dramatic increase in immature CD 44 ( + ) CD 25 ( ) cells , a decrease in the more mature CD 4 ( + ) CD 8 ( + ) cells , and development of an abnormal CD 44 ( + ) CD 8 ( + ) population . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Development of thymocytes can be staged according to the levels of expression of the cell surface markers CD 4 , CD 8 , CD 44 , CD 25 and CD 2 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
A small fraction of rat 13 day old fetal liver ( r13FL ) cells , which weakly expressed the leukocyte common antigen CD 45 , constituted a homogeneous Thy 1 ( hi ) , CD 71 ( ) , CD 44 ( + ) , MHC class I+ , CD 43 ( + ) cell subpopulation negative for CD45RC , CD 3 , TCRalphabeta , TCRgammadelta , CD 2 , CD 5 , CD 4 , CD 8 , CD 25 , CD 28 , NKR P1a and sIg . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
FPM 1 originated from rat thymocytes cocultured with a human HTLV 1 producer , MT 2 cells , and expressed rat CD 4 , CD 5 , CD 25 , and HTLV 1 Tax . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Immunochemical analysis showed that CD 3 , CD 4 , CD 25 , and TCR beta were positive , and in situ hybridization revealed Epstein Barr virus encoded small RNAs in the nuclei of the lymphoma cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In order to investigate the possibility of whether or not the lymphocytes of patients with Alzheimer ' s Disease ( AD ) are in an activated state , blood mononuclear cells from 45 AD patients and 45 healthy age matched controls were immunophenotyped by measuring the expression of CD 3 , CD 4 , CD 7 , CD 8 , CD 25 , CD 28 , CD 56 and HLA DR by flow cytometry . ^^^ A significant increase in the CD 4 , CD 25 and CD 28 antigen expression was also observed in the AD group ( 55 . 3 % 24 . 8 % and 65 . 1 % ) with respect to healthy subjects ( 44 . 5 % , 10 . 3 % and 54 . 3 % ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In addition , altered thymocyte development in TAM 67 expressing mice was revealed by a decrease in thymic cellularity ( approximately 50 % ) which could be accounted for primarily by a reduction in the number of CD 4 ( + ) CD 8 ( + ) thymocytes , a large percentage of which retained CD 25 . ^^^ The decrease in the number of CD 4 ( + ) CD 8 ( + ) thymocytes did not appear to be due to an enhanced rate of apoptosis but rather to a decrease in the number of CD 4 ( ) CD 8 ( ) CD 25 ( ) cells in the S + G ( 2 ) / M stages of the cell cycle . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We investigated the effects of acute injection of morphine into the rat mesencephalon periaqueductal gray ( PAG ) , on splenic natural killer ( NK ) cell and lymphocyte functions , interleukin 2 ( IL 2 ) production , expression of T cell ( CD 3 ) , T helper cell ( CD 4 ) , T suppressor cell ( CD 8 ) , and NK cell ( NKR P 1 ) surface markers , and expression of IL 2 ( CD 25 ) and transferrin ( CD 71 ) receptors . ^^^ Furthermore , morphine did not alter the expression of CD 3 , CD 4 , CD 8 , and NKR P 1 surface markers , but significantly ( p < 0 . 001 ) downregulated the expression of CD 25 and CD 71 receptors following in vitro activation . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Recombinant IL 16 was found to up regulate CD 25 and CD 80 but to down regulate CD 4 and CD 86 surface expression in MDM cultures . ^^^ However , no change could be observed on the level of CD 4 , CD 80 and CD 86 expression in IL 16 stimulated MDDC , although a significant up regulation of CD 25 and CD 83 was consistently detected . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We show that the majority of PU . 1 / thymocytes are blocked in differentiation prior to T cell commitment , and contain a population of thymocyte progenitors with the cell surface phenotype of CD44+ , HSAbright , c kitint , Thy 1 , CD 25 , Sca 1 , CD 4 , and CD 8 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
No significant differences among the groups were found in the values of CD 2 ( total T cells ) , CD 4 ( T helper / inducer subset ) , CD8dim ( T cytotoxic subset ) , CD4 / CD8 ratio , HLA DR ( B cells and activated T cells ) , CD 20 ( total B cells ) and CD 25 ( T activated lymphocytes with expression of the alpha chain of interleukin 2 receptor ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In bone marrow of ALL patients , T cells do not express CD40L and CD 25 markers , their apoptosis rate is increased , and a predominance of a CD 4 cell subset expressing a Th 2 phenotype is detected . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Their mucosal CD 3 , CD 4 , CD 25 , EG 1 and EG 2 positive cell counts were intermediate between those of the control subjects and asthmatics . ^^^ At this time , bronchial biopsies revealed an increase in the extent of subepithelial fibrosis and in the number of CD 25 and CD 4 positive cells , and a decrease in the number of CD8+ cells , particularly in subjects who developed asthma symptoms . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Interleukin 16 is a soluble ligand to the CD 4 molecule with chemotactic properties for CD4+ cells and a competence growth factor for CD4+ T cells , upregulating HLA DR and the interleukin 2 receptor CD 25 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Approximately 43 45 % ( median of cells in each tumour ) of both the CD 4 ( + ) and the CD 8 ( + ) TILs expressed HLA class 2 ; 14 . 2 % of the CD 4 ( + ) TILs expressed CD 25 and none of the CD 8 ( + ) TILs expressed CD 25 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
During adult thymus development immature CD 4 ( ) CD 8 ( ) [ double negative ( DN ) ] precursor cells pass through four phenotypically distinct stages defined by expression of CD 44 and CD 25 : CD 44 ( hi ) CD 25 ( ) ( DN 1 ) , CD 44 ( hi ) CD 25 ( + ) ( DN 2 ) , CD 44 ( lo ) CD 25 ( + ) ( DN 3 ) and CD 44 ( lo ) CD 25 ( ) ( DN 4 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
T lymphocytes ( CD 3 ) , T lymphocyte subpopulations ( CD 4 and CD 8 ) , B lymphocytes ( CD 19 ) , natural killer cells ( CD16+56 ) and activation markers of T and B lymphocytes ( CD 23 and CD 25 ) were determined using flow cytometry . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Inhibition was not associated with altered cell surface expression of receptors such as CD 3 , CD 4 , ICAM 1 , LFA 1 , CD 25 , and HLA DR however , these antibodies did impede the ability of generation of functionally active T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We show that p 56 ( lck ) transcripts are detectable in each CD 4 ( ) CD 8 ( ) double negative ( DN ) thymocyte subset defined by CD 25 and CD 44 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Increases in the total numbers of cells expressing CD 25 ( interleukin 2 receptor ) , VLA 4 and the combinations of CD 4 : CD45RA and CD 4 : CDw 29 were not evident during therapy but were seen at 15 months ( P < 0 . 05 : CD 25 , CD 4 : CDw 29 , CD 4 : CD45RA ; P < 0 . 001 : VLA 4 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
After 7 days of proliferation in response to stimulation by HA peptide or whole influenza vaccine , cells staining positive with the HA tetramer had undergone between 6 and 9 divisions and were CD 3 ( + ) , CD 4 ( + ) , CD 25 ( + ) , and CD 8 ( ) , characteristic of activated T helper cells responding to antigen . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The percentages of CD 4 ( + ) cells coexpressing activation markers HLA DR and CD 25 , and of CD 8 ( + ) cells expressing HLA DR , were significantly higher in HAM patients and carriers than in controls . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The gross morphological damage score was evaluated and the expression of CD 4 , CD 8 , CD11a , CD11b , CD 25 , CD 54 and class 2 major histocompatibility complex ( MHC ) antigen was investigated by immunohistochemical methods . 3 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
There was no significant difference between elderly group and controls in CD 2 , CD 3 , CD 4 , CD 8 , CD 19 , CD 25 and HLA DR antigen distribution . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 25 expression , which is associated with proliferation , was static or slightly down regulated in CD 4 ( + ) T cells from both peripheral blood ( PB ) and lymph nodes ( LN ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
K 2 MDS cells are positive for the expression of CD 4 , CD 5 , CD 13 , CD 25 , CD 71 , CD 95 , HLA DR and cytoplasmic CD 3 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Moreover , leptin dose dependently enhanced the expression of the late activation markers CD 25 and CD 71 in both CD 4 ( + ) and CD 8 ( + ) T lymphocytes after 48 h stimulation with PHA or Con A . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Expression of CD 3 , CD 4 , CD 8 , CD 25 , CD 38 , CD 56 , CD 71 , CD 122 , and HLA DR antigens , serum level of the soluble IL 2R alpha , and NK activity against K 562 cell line were evaluated in 11 patients representative for both types of immunotherapy . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
BALF and blood were prepared for cell differential count and flow cytometry analysis using monoclonal antibodies CD 3 , CD 4 , CD 8 , CD 25 , HLA DR and gamma / delta as well as alpha / beta T lymphocyte receptors . ^^^ The absolute number of total lymphocytes , CD3+ lymphocytes and CD3+ gamma / delta T lymphocytes were significantly higher in the BALF , but not in the blood , of patients with TB , however , the proportions of CD3+ gamma / delta T lymphocytes in BALF of patients with TB was comparable to that of normal controls . gamma / delta T lymphocytes in the BALF rarely expressed CD 4 , CD 25 , and HLA DR in both groups . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Lymphocytes subsets ( CD 3 , CD 4 , CD 8 ) and their activation status ( CD 25 ) in bronchoalveolar lavage were also measured . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Furthermore , small MOPC 315 tumors established in Id specific TCR TG mice contained clusters of activated ( CD 69 ( + ) CD 25 ( + ) ) and proliferating ( BrdUrd ( + ) ) Id specific transgenic CD 4 ( + ) blasts . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 3 ( epsilon ) transgenic mice , RAG ( null ) mice and RAG ( null ) bone marrow transplanted CD 3 ( epsilon ) transgenic mice , that the control point in lymphoid development where triple negative ( CD 3 ( ) , CD 4 ( ) , CD 8 ( ) ) thymocytes progress from CD 44 ( + ) CD 25 ( ) towards CD 44 ( ) CD 25 ( + ) , influences the development of epithelial cells , critically inducing the extra , third dimension in the organization of the epithelial cells in the cortex . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
BAL lymphocytes were phenotyped using monoclonal antibodies coupling CD 4 , CD 8 ( both in coexpression with CD 25 ) , CD 19 and HLA DR human antigens and flow cytometer FACStar ( Becton Dickinson ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
At 4 weeks after transplantation , absolute lymphocytes ( 0 . 48 vs 0 . 63 , p=0 . 08 ) and CD 25 cells ( 0 . 04 vs 0 . 08 , p=0 . 007 ) were higher in the PBSC group , and the proportion of patients with absolute lymphopenia ( 74 % vs 33 % , p=0 . 03 ) and CD 4 lymphopenia ( 59 % vs 24 % , p=0 . 05 ) was significantly higher in the bone marrow group . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 25 upregulation on peptide stimulated CD 4 ( + ) CD 8 ( + ) cells dominated by Th memory cells in the pig and inhibition using anti major histocompatibility complex class 2 monoclonal antibodies indicated recognition by Th lymphocytes . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Flow cytometric analysis then revealed that LPS stimulation in vitro induced the expression of an IL 2 receptor ( CD 25 ) on CD 4 T cells ; this indicates that the activation of islet specific T cells is a prerequisite to eliciting diabetes in this situation . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Phenotype was studied using flow cytometry for a varity of human immunocompetent cell surface markers ( CD 3 , CD 4 , CD 8 , CD 25 ) . ^^^ RESULTS : ( 1 ) There was no significant difference in CD 3 , CD 4 , CD 8 and CD 25 expression among the three groups ( P > 0 . 05 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
BAL lymphocytes subpopulations ( CD 3 , CD 19 , NK , CD 4 , CD 8 , HLA DR , CD 25 , ) were estimated by flow cytometry . ^^^ Also the mean values of absolute number of lymphocytes and lymphocytes CD 4 , HLA DR , CD 25 in ND group were significantly higher in BAL from s . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The phenotype of tumor cells revealed CD7+ , CD5+ , CD2+ , CD3+ , WT 31 , TcR delta 1 , CD 4 , CD 8 , CD 25 , and the karyotype showed a 5q , t ( 12 ; 18 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
More relevant were the changes in the CD 4 ( + ) / CD8 ( + ) T cell ratio , particularly for cells bearing the CD 25 ( interleukin 2 receptor alpha ) marker . ^^^ In fact , a progressively increased number of both CD 4 ( + ) alpha / beta TCR and CD 4 ( + ) CD 25 ( + ) VL was observed after the second and third Candida challenges , reversing the high initial CD 8 ( + ) cell number of controls ( estrogenized but uninfected rats ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Expression of CD 3 , CD 4 , CD 8 , CD 56 , CD1a , CD 68 , CD 25 , HLA DR , CD 54 , perforin , granzyme B , IL 12 and IFNgamma was analysed using immunohistochemistry . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We have addressed this problem in colorectal cancer at systemic level by examining the proliferative response of peripheral blood mononuclear cells ( PBMC ) to interleukins ( IL ) IL 2 , IL 4 , antiCD 3 monoclonal antibody ( antiCD 3 ) , IL 2+CD3 , IL 2+IL 4 , IL 4+CD3 , IL 2+CD3+IL 4 ; the PBMC expression of phenotypic antigens CD 3 , CD 4 , CD 8 , CD 25 , DR , CD 16 , CD 56 , CD 57 and CD 19 in the patients and healthy subjects as control group . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
These involved ( a ) increased plasma levels of neopterin and sTNF receptor indicating elevated IFNgamma and TNF cytokine activity ; ( b ) increased proportion of mature ( CD45RO ) versus nave ( CD45RA ) T cells ; and ( c ) a diverse group of related changes including impaired proliferative response , reduced T cells , CD 28 and CD 25 expression , B cell percentage and lower CD 4 : CD 8 ratios and increased HLA DR expression . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Although displaying normal development of CD 2 , CD 3 , CD 4 , and CD 8 expression , CD 25 deficient cortical thymocytes do not express CD 1 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Thymuses of young transgenics showed an increased number of thymocytes , particularly late CD 4 ( ) 8 ( ) cells , a failure to down regulate CD 25 in post CD 4 ( ) 8 ( ) subsets and sustained activity of NF kappaB . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Treatment resulted in a decrease in the concentration of myelin basic protein , a decrease in the serum concentration of immunoglobulin G ( IgG ) and intrathecal IgG synthesis , an increase in the cerebrospinal fluid concentration of transforming growth factor beta 1 , and changes in the expression of CD 25 , CD 26 , and human leukocyte antigen DR ( HLA DR ) on CD 4 T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Significant increases in the percentage of CD 4 ( + ) T cells co expressing MHC Class 2 , but not CD 25 , were observed in the larval infected groups except in adult nematode infected sheep . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Newly expressed surface antigens such as CD 25 , CD 4 , and CD 69 were observed on peripheral blood eosinophils in five cases . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Cytotoxic T lymphocyte associated antigen 4 plays an essential role in the function of CD 25 ( + ) CD 4 ( + ) regulatory cells that control intestinal inflammation . ^^^ Here , we report that the Treg cells that control intestinal inflammation express the same phenotype ( CD 25 ( + ) CD45RB ( low ) CD 4 ( + ) ) as those that control autoimmunity . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Immunologic self tolerance maintained by CD 25 ( + ) CD 4 ( + ) regulatory T cells constitutively expressing cytotoxic T lymphocyte associated antigen 4 . ^^^ In normal naive mice , CTLA 4 is constitutively expressed on CD 25 ( + ) CD 4 ( + ) T cells , which constitute 5 10 % of peripheral CD 4 ( + ) T cells . ^^^ When the CD 25 ( + ) CD 4 ( + ) T cells are stimulated via the T cell receptor in vitro , they potently suppress antigen specific and polyclonal activation and proliferation of other T cells , including CTLA 4 deficient T cells , and blockade of CTLA 4 abrogates the suppression . ^^^ CD 28 deficient CD 25 ( + ) CD 4 ( + ) T cells can also suppress normal T cells , indicating that CD 28 is dispensable for activation of the regulatory T cells . ^^^ Thus , the CD 25 ( + ) CD 4 ( + ) regulatory T cell population engaged in dominant self tolerance may require CTLA 4 but not CD 28 as a costimulatory molecule for its functional activation . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Cryosections of corneas were immunostained with a panel of monoclonal antibodies ( mAb ) against CD 3 , CD 4 , CD 8 , CD 14 , CD 25 , CD 68 , HLA DP , and HLA DR molecules using the StreptABC method . ^^^ Weak positivity for CD 4 and CD 25 was observed in six of 15 and 11 of 15 rejected corneas , respectively . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Additionally , the appearance of several activation ( CD 25 , CD 69 , HLA DR ) and adhesion ( CD11a , CD11b , CD11c , CD 18 , CD49b , CD49d , CD 54 ) receptors on CD 4 cells were analyzed . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The patient ' s peripheral blood lymphocyte derived cultured T cell line had a CD 3 ( + ) Vbeta 22 ( + ) CD 4 ( + ) CD8alphaalpha ( + ) CD 25 ( ) phenotype . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Naturally anergic and suppressive CD 25 ( + ) CD 4 ( + ) T cells as a functionally and phenotypically distinct immunoregulatory T cell subpopulation . ^^^ Here we show that the expression of CD 25 is highly specific , when compared with other molecules , in delineating the autoimmune preventive immunoregulatory CD 4 ( + ) T cell population . ^^^ Furthermore , although CD 25 is an activation marker for T cells , the following findings indicate that immunoregulatory CD 25 ( + ) CD 4 ( + ) T cells are functionally distinct from activated or anergy induced T cells derived from CD 25 ( ) CD 4 ( + ) T cells . ^^^ Second , such activated or anergy induced CD 25 ( ) spleen cells produce various autoimmune diseases when transferred to syngeneic athymic nude mice , whereas similarly treated normal spleen cells , which include CD 25 ( + ) CD 4 ( + ) T cells , do not . ^^^ Third , upon polyclonal T cell stimulation , CD 25 ( + ) CD 4 ( + ) T cells express CD 25 at higher levels and more persistently than CD 25 ( ) CD 4 ( + ) T cell derived activated T cells ; moreover , when the stimulation is ceased , the former revert to the original levels of CD 25 expression , whereas the latter lose the expression . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Functionally , 4 1BB was a potent stimulator of proliferation , cytokine secretion , and CD 25 expression by CD 8 T cells , but 4 1BB signals had a weak effect on the proliferation of CD 4 T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
T595B1 expressed CD 3 , T cell receptor ( TCR ) beta , TCR Vbeta 2 , CD 4 , CD 25 , CD45RB , CD 44 , LFA 1 , and ICAM 1 molecules on its cell surface and showed MHC class 2 1 E ( k ) restricted tumor antigen specific proliferation . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Changes in CD 25 ( + ) cells and in CD 4 ( + ) cells expressing class 2 MHC also correlated with changes in disability as measured by EDSS in patients with relapsing remitting disease , and changes in CD 4 ( + ) CD 25 ( + ) cells correlated with the occurrence of attacks in patients with relapsing remitting disease . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
IL 16 expression was correlated to numbers of CD 4 ( + ) cells , CD 25 ( + ) cells , and activated eosinophils . ^^^ IL 16 expression correlated with the numbers of CD 4 ( + ) cells and CD 25 ( + ) cells after allergen challenge in asthmatic subjects with a provocative concentration required to decrease the FEV ( 1 ) by 20 % of its baseline value ( PC ( 20 ) FEV ( 1 ) ) < 4 mg / mL . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Flow cytometric analyses and cell sorting experiments identified T helper cells as the main target responding to inactivated poxviruses : the activated cells had a CD 4 ( high ) CD 25 ( + ) major histocompatibility complex type 2 positive phenotype and were the major source of secreted cytokines . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Immunohistochemical analysis revealed that lymphoid CD 4 ( + ) and CD 8 ( + ) T cells as well as macrophages accumulated outside untreated tumour nodes while CD 8 ( + ) and CD 25 ( + ) activated T cells and macrophages heavily infiltrated the tumours after the different treatments . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Newly expressed surface antigens CD 4 , CD 25 , CD 69 , and HLA DR , but not CD 54 , were observed on peripheral blood eosinophils . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
During the experiment clinical observations and measurements were made and when the animals were killed , blood was sampled for haematological and immunological assays ( CD 4 , CD 8 , CD 25 T cells and NK cells , antinuclear autoantibody and immune complexes ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
After performing immunological phenotyping of lymphocytes we have found some alterations in the patient ' s immune status : increased T CD 4 : TCD 8 ratio , increased percentage of B CD19+ and B CD19+5+ lymphocytes , increased percentage of Natural Killer cells CD 3 ( ) 16 / 56+ , deficiency of T CD8+ suppressor lymphocytes and increased expression of CD 25 and HLA DR antigens on T CD4+ lymphocytes . ^^^ After forty days of prednisone therapy in the dose mentioned above patient ' s immunological status has evolution favorably : T CD 4 : TCD 8 ratio decreased , percentage of B CD19+ and B CD19+5+ lymphocytes decreased , percentage of Natural Killer cells CD 3 ( ) 16 / 56+ decreased , the expression of CD 25 and HLA DR antigens on T CD4+ lymphocytes decreased . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
BAL lymphocyte subsets were phenotyped by direct two and three color immunofluorescence ( applied DAKO A / S monoclonal antibodies : anti CD 3 , CD 4 , CD 8 , CD11b , CD 14 , CD 15 , CD 16 + 56 , CD 19 , CD 25 , CD 45 , HLA DR ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Baseline mean bronchial cell counts ( per mm ( 2 ) connective tissue surface ) for CD 3 ( + ) , CD 4 ( + ) , CD 8 ( + ) , CD 25 ( + ) , EG 1 ( + ) , CD45ro ( + ) , and AA 1 ( + ) cells were similar in both groups . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The proliferative response was defined as a Th response by the selective expansion of CD 4 ( + ) cells , up regulation of CD 25 and CD40L , and IL 2 and IFN gamma expression . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Furthermore , patients with pltc < 50 10 10 ( 9 ) / l and splenectomised patients had significantly higher levels of CD 4 + CD 25 + activated T cells [ 26 . 2 + / 14 . 8 % ( P < 0 . 05 ) and 26 . 7+ / 11 . 9 % ( P < 0 . 005 ) , respectively ] than healthy controls ( 16 . 5+ / 4 . 0 % ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Increased expression of CD 25 and the Th 1 associated chemokine receptor CCR 5 was also seen on CD 4 ( + ) and CD 8 ( + ) PP T cells , but not blood T cells , stimulated with betalg . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Analysis within the immature CD 4 ( ) 8 ( ) thymocyte subset further revealed an alteration in normal CD 44 and CD 25 subset distribution . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
No intergroup differences were found in the surface expression of HLA DR , CD 25 , CCR 5 , CXCR 4 , CCR 3 on CD 4 T cells , or monocytes before infection . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Suppression was abolished by the elimination of CD 25 ( + ) T cells in the tolerant CD 4 ( + ) T cell subset . ^^^ CD 25 ( + ) CD 4 ( + ) T cells suppressed the proliferative response of the residual fraction of the nonanergic population , namely , 6C10 ( + ) CD 4 ( + ) T cells still present in the tolerant mice . ^^^ However , 6C10 ( ) CD 4 ( + ) T cells still had reduced reactivity to Mls 1 ( a ) even after CD 25 ( + ) CD 4 ( + ) T cells were removed and exogenous IL 2 was added . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We present a rare case of adult T cell leukemia / lymphoma ( ATL ) in which leukemic T cells expressed CD 4 and CD 25 surface antigens and infiltrated mammary glands during clinical course of the disease . ^^^ Peripheral blood lymphocytes were highly pleomorphic and presented CD 2 , CD 4 , CD 25 , CD 38 membrane surface antigens . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The primary phase of infection was associated with changes in the T cells expressing CD 25 , CD 69 , or HLA DR and with a priming of the peripheral blood CD 4 and CD 8 T cells for a process of apoptosis in vitro that was enhanced by CD 95 ( Fas ) ligation , and was detected in two macaques as early as 7 days after infection . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The expanded CD 4 ( + ) cell population expressed CD 25 ; however , in contrast to concanavalin A treated culture , delayed CD 44 expression was observed . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The main outcome measures were lymphocyte subset and activation markers determined by two and three color flow cytometry ( CD 2 , CD 3 , CD 4 , CD 5 , CD 8 , CD 16 , CD 19 , CD 23 , CD 25 , CD 56 , and HLA DR ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Skin biopsies were taken at the same time points , fixed , sectioned , and stained with Giemsa for histologic evaluation , or with mouse anti swine monoclonal antibodies ( CD 1 , CD 2 , CD 4 , CD 5 , CD 8 , CD 25 , CD 45 , MHCII ) and with one mouse anti human monoclonal antibody ( CD62E ) cross reacting with swine for immunohistochemical evaluation . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Results showed that tucaresol : ( 1 ) increases influenza A virus , gp 160 peptide , and HLA alloantigen stimulated proliferation as well as interleukin ( IL ) 2 and interferon gamma ( IFN gamma ) production by PBMC of HIV infected individuals with higher CD 4 counts ( > 500 / microl ) but had only a marginal immunomodulatory effect on PBMC of patients with lower CD 4 counts ( < 500 / microl ) ; ( 2 ) did not modify IL 10 production ; ( 3 ) augmented CD 25 expression on mitogen stimulated T cells of HC but not of HIV infected individuals ; and ( 4 ) marginally increased CTL activity . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In the CD 25 depleted cultures , IL 2 treatment ( 100 IU / ml ) increased the percentage of CD 4 T cells expressing CD 25 by 30 . 6 % ( P = 0 . 05 ) and of CD 8 T cells by 48 . 2 % ( P = 0 . 01 ) on day 7 compared to no treatment . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In gut associated lymphoid tissues of wild type mice , only CP harbored a large population of c kit ( high ) IL 7R ( + ) CD 44 ( + ) Thy 1 ( + / ) CD 4 ( + / ) CD 25 ( low / ) alpha ( E ) beta ( 7 ) ( ) Lin ( ) ( Lin , lineage markers ) lymphocytes that included cells expressing germline but not rearranged TCRgamma and TCRbeta gene transcripts . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The development of thymocytes in the transgenic strain in which p 27 ( Kip 1 ) is most abundant ( p 27 Tg ( high ) mice ) appeared to be blocked at the CD 4 ( ) CD 8 ( ) CD 25 ( + ) CD 44 ( low ) stage . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In CD 40 deficient mice , the CD 25 ( + ) CD45RB ( low ) CD 4 ( + ) subpopulation which regulates T cell autoreactivity was markedly reduced . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The lymphocyte subsets studied were : T ( CD 3 , CD 4 , CD 8 ) , B ( CD 19 , CD 23 ) , natural killer ( NK ) ( CD 56 , CD 16 ) , and interleukin 2 ( CD 25 ) . ^^^ After NC there was a significant percentage increase in CD 3 , CD 4 , CD 8 , CD 25 and CD3+CD56+ cells and a decrease in CD 19 , CD 23 , CD 56 , CD 16 and CD16+CD8+ cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) Thy 1 ( ) thymocytes express high levels of CD 3 , intermediate to high levels of heat stable antigen ( HSA ) , and low levels of CD 25 , CD45RB , CD 69 , CD 44 and CD62L . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We found a numeric decrease in helper T cells ( higher in CD 4 ( + ) CD45RA ( + ) than in CD 4 ( + ) CD 29 ( + ) ) and B cells , and a rise in activated , CD 4 ( + ) CD 25 ( + ) lymphocytes that was correlated with lymphocyte depletion . ^^^ In addition , we performed striatal dopamine depletion in rats with either MPP ( + ) or 6 OHDA , showing that MPP ( + ) but not 6 OHDA can increase CD 4 ( + ) CD 25 ( + ) lymphocytes . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Sensitized monkeys exhibited increased levels of HDMA specific IgE in serum , numbers of eosinophils and exfoliated cells within lavage , and elevated CD 25 expression on circulating CD 4 ( + ) lymphocytes . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In human PBMNC jaundice reduced the percentages of CD 3 , CD 5 , CD 4 , CD 8 and HLA DR expressing cells and increased the proportion of CD 25 and perforin positive lymphocytes . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In EBV seropositive individuals , the phenotype of the EBV specific cytokine producing T cells was evaluated using four color flow cytometry and found to be CD 45 ( + ) , CD 3 ( + ) , CD 4 ( + ) , CD45RA ( ) , CD 69 ( + ) , CD 25 ( ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Noninfectious CXCR 4 tropic HIV 1 virions , but not microvesicles , partially activated freshly isolated CD 4 ( + ) and CD 8 ( + ) peripheral blood mononuclear cell T lymphocytes to express FasL and Fas , but not CD 69 or CD 25 ( interleukin 2 receptor alpha ) and eventually die via apoptosis starting 4 to 6 days postexposure . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Because there is no age associated decrease in the number of CD 44 ( + ) CD 25 ( ) CD 3 ( ) CD 4 ( ) CD 8 ( ) cells , this study investigated whether alterations in apoptosis within the TN pathway accounted for diminishing thymocyte numbers with age . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In N15tg RAG 2 ( / ) CD 2 ( / ) mice , thymic dysfunction is evident by 6 wk with a pre TCR block in the CD 4 ( ) CD 8 ( ) double negative thymocytes at the CD 25 ( + ) CD 44 ( ) stage . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We have therefore performed an immunohistochemical study with a large panel of monoclonal antibodies ( to CD 3 , CD 4 , CD 8 , CD 25 , CD 30 , myeloperoxidase , eosinophil cationic protein EG 2 , tryptase , human interleukin ( IL ) 2 , human IL 4 , human IL 5 , human IL 6 , human IL 8 , and interferon ( IFN ) gamma using the alkaline phosphatase antialkaline phosphatase procedure on lesional and uninvolved skin of six patients with clinical , histological , and immunofluorescent proven pemphigus . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Functional characterization of a swine CD 4 ( + ) / CD8 ( + ) double positive lymphoblastoid T cell line with a CD 25 ( + ) / CD45RA ( ) phenotype generated in vitro with interleukin 2 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Here , we show that the CD 25 ( + ) subpopulation of naturally activated ( CD45RB ( low ) ) CD 4 T cells , but not CD 25 ( ) CD45RB ( low ) CD 4 T cells , inhibits the accumulation of cotransferred CD45RB ( high ) CD 4 T cells in lymphocyte deficient mice . ^^^ However , both CD 25 ( + ) and CD 25 ( ) CD45RB ( low ) CD 4 T cell subpopulations contain regulatory cells , since they can prevent naive CD 4 T cell induced wasting disease . ^^^ CD 25 ( + ) CD45RB ( low ) CD 4 T cells from IL 10 deficient mice do not protect from disease . ^^^ Although CD 25 ( + ) CD45RB ( low ) CD 4 T cells are capable of expanding when transferred in vivo , they reach a homeostatic equilibrium at lower cell numbers than CD 25 ( ) CD45RB ( low ) or CD45RB ( high ) CD 4 T cells . ^^^ We conclude that CD 25 ( + ) CD45RB ( low ) CD 4 T cells from nonmanipulated mice control the number of peripheral CD 4 T cells through a mechanism involving the production of IL 10 by regulatory T cells . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In contrast , the CD45RB ( low ) CD 4 ( + ) and CD 25 ( + ) CD 4 ( + ) populations failed to induce rejection , but rather , were able to inhibit rejection initiated by naive CD45RB ( high ) CD 4 ( + ) T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
There was near unanimity that additional markers ( selected from CD 22 , FMC 7 , CD11c , CD 103 , CD 38 , CD 25 , CD79b and heavy chains for B cell disorders , and CD 4 , CD 7 , CD 8 , CD 2 , CD 56 , CD 16 , TCRa / b , and TCRg / d for T cell disorders ) would be needed to fully characterize CLD , although not every marker would be useful in all cases . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Autoreactive T cells specific to these autoantigens are present in most normal individuals but are kept under control by a number of peripheral tolerance mechanisms , among which CD 4 ( + ) CD 25 ( + ) CD62L ( + ) T cell mediated regulation probably plays a central role . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
This is most apparent in regulation of the CD 4 ( + ) CD 25 ( + ) CTLA 4 ( + ) immunoregulatory T cells that control multiple autoimmune diseases . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Differential cytokine requirements for regulation of autoimmune gastritis and colitis by CD 4 ( + ) CD 25 ( + ) T cells . ^^^ Using RAG 2 ( / ) mice as recipients , we determined that the induction of disease relies on CD 4 ( + ) CD 25 ( ) effector cells and prevention relies on CD 4 ( + ) CD 25 ( + ) regulatory cells ; neither requires participation of CD 8 cells or B cells . ^^^ The severity of gastritis was dependent on the cytokine repertoire of CD 4 ( + ) CD 25 ( ) effector T cells . ^^^ Protection from gastritis does not require either IL 4 or IL 10 because CD 4 ( + ) CD 25 ( + ) cells from IL 4 ( / ) or IL 10 ( / ) mice completely abrogated the disease process . ^^^ CD 4 ( + ) CD 25 ( + ) cells also protected RAG 2 ( / ) recipients from colitis and inhibitory activity was partially dependent on IL 10 expression . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Here we show that the thymic T progenitor cells ( CD 25 ( + ) CD 44 ( + ) c kit ( + ) CD 3 ( ) CD 4 ( ) CD 8 ( ) thymocytes , termed pro T cells ) from young adult mice that have yet to express TCRs can be subdivided based on interleukin 7 receptor ( IL 7R ) expression . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Second , the development of CD 4 ( + ) CD 25 ( + ) immunoregulatory T cells was impaired in Stat5a ( / ) mice , as indicated by a significant decrease in the number of CD 4 ( + ) CD 25 ( + ) T cells in Stat5a ( / ) mice . ^^^ Furthermore , the depletion of CD 4 ( + ) CD 25 ( + ) T cells from wild type splenocytes significantly decreased Th 2 cell differentiation but increased Th 1 cell differentiation , whereas the depletion of CD 4 ( + ) CD 25 ( + ) T cells from Stat5a ( / ) splenocytes had no significant effect on the Th 1 and Th 2 cell differentiation . ^^^ Together , these results indicate that the intrinsic expression of Stat5a in CD 4 ( + ) T cells is required for Th 2 cell differentiation and that Stat5a is involved in the development of CD 4 ( + ) CD 25 ( + ) immunoregulatory T cells that modulate T helper cell differentiation toward Th 2 cells . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Human anergic / suppressive CD 4 ( + ) CD 25 ( + ) T cells : a highly differentiated and apoptosis prone population . ^^^ Anergic / suppressive CD 4 ( + ) CD 25 ( + ) T cells exist in animal models but their presence has not yet been demonstrated in humans . ^^^ We have identified and characterized a human CD 4 ( + ) CD 25 ( + ) T cell subset , which constitutes 7 10 % of CD 4 ( + ) T cells in peripheral blood and tonsil . ^^^ Depletion of this small subset from CD 4 ( + ) T cells significantly enhances proliferation by threefold in the remaining CD 4 ( + ) CD 25 ( ) T cells , while the addition of isolated CD 4 ( + ) CD 25 ( + ) T cells to CD 4 ( + ) CD 25 ( ) T cells significantly inhibits proliferative activity . ^^^ Isolated CD 4 ( + ) CD 25 ( + ) T cells are susceptible to apoptosis that is associated with low Bcl 2 expression , but this death can be prevented by IL 2 or fibroblast secreted IFN beta . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
However , these observations still leave open the important issue of whether CD 25 cleavage , and the consequent suppression of IFN gamma secretion , leads to enhanced IL 4 secretion , and whether such cytokine changes would be exhibited by both CD 4 and CD 8 T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Human CD 4 ( + ) CD 25 ( + ) thymocytes and peripheral T cells have immune suppressive activity in vitro . ^^^ CD 4 ( + ) CD 25 ( + ) T cells in mice and rats are capable of transferring protection against organ specific autoimmune disease and colitis and suppressing the proliferation of other T cells after polyclonal stimulation in vitro . ^^^ Here we describe the existence in humans of CD 4 ( + ) CD 25 ( + ) T cells with the same in vitro characteristics . ^^^ CD 4 ( + ) CD 8 ( ) CD 25 ( + ) T cells are present in both the thymus and peripheral blood of humans ( approximately 10 % of CD 4 ( + ) CD 8 ( ) T cells ) , proliferate poorly in response to mitogenic stimulation and suppress the proliferation of CD 4 ( + ) CD 25 ( ) cells in co culture . ^^^ CD 4 ( + ) CD 25 ( + ) cells from thymus and blood were poor producers of IL 2 and IFN gamma , and suppressed the levels of these cytokines produced by CD 4 ( + ) CD 25 ( ) cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The HSR attenuated leukocyte infiltration into CNS assessed by quantitation of perivascular infiltrates , and by reduced staining for CD 4 and CD 25 immunopositive T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Isolated peripheral blood lymphocytes were stained for the lymphocyte subset markers CD 4 , CD 8 , CD 19 , their respective activation markers CD 25 , HLA DR , CD 38 , and the costimulatory molecules CD40L , CD 28 , CD 40 , CD 80 , and CD 86 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
METHODS : Skin biopsy specimens from drug induced maculopapular exanthema and normal skin were analyzed for CD 4 , CD 8 , CD 25 , HLA DR , CD 54 , perforin , granzyme B , IL 5 and IFN gamma using immunohistochemistry . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Concentration of soluble CD 4 , CD 8 and CD 25 receptors in early localized and early disseminated Lyme borreliosis . ^^^ BACKGROUND : The aim of this study was to evaluate the concentrations of soluble CD 4 , CD 8 and CD 25 antigens in the serum of patients with early LocaLized , ( group 1 , n = 22 patients ) and early disseminated Lyme borreliosis ( group 2 n = 23 patients ) , before and after antibiotic therapy compared with controls ( group 3 n = 7 healthy persons ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Rather , evidence is provided that the pTalpha cytoplasmic tail also causes a reduced surface expression of individual membrane molecules such as CD 25 and CD 4 , which are shown to be retained in the endoplasmic reticulum ( ER ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We have in this report studied the early T cell development in these mice and we show that the transgenic TCRalpha chain is expressed by precursor thymocytes already at the CD 3 ( ) CD 4 ( ) CD 8 ( ) ( triple negative , TN ) CD 44 ( + ) CD 25 ( ) stage of development . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The total number of thymocytes was profoundly reduced in the transgenic mice , whereas in the double negative ( CD 4 ( ) CD 8 ( ) ) thymocyte subset , in particular the CD 25 ( + ) CD 44 ( ) pre T cell population , it was significantly increased . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Control of T cell hyperactivation in IL 2 deficient mice by CD 4 ( + ) CD 25 ( ) and CD 4 ( + ) CD 25 ( + ) T cells : evidence for two distinct regulatory mechanisms . ^^^ Interestingly however , regulatory CD 4 ( + ) CD 25 ( + ) cells also efficiently contain the clone size of antigen stimulated IL 2 deficient T cells . ^^^ Since CD 4 ( + ) CD 25 ( + ) cells do not produce IL 2 , this suggests a mechanism of suppression distinct from paracrine IL 2 delivery . ^^^ In keeping with this , the residual IL 2 ( ) TCRtg cells recovered after cotransfer of regulatory CD 4 ( + ) CD 25 ( + ) cells do not show increased CD 25 or CD 69 expression , suggesting that they had not received paracrine IL 2 and that clonal containment occurred at the level of initial activation rather than clonal contraction by AICD . ^^^ IL 2 deficiency therefore may upset T cell homeostasis by two distinct mechanisms : the failure to program expanding T cells for apoptosis , and the failure to generate functional CD 4 ( + ) CD 25 ( + ) regulatory cells . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Identification and functional characterization of human CD 4 ( + ) CD 25 ( + ) T cells with regulatory properties isolated from peripheral blood . ^^^ A subpopulation of peripheral human CD 4 ( + ) CD 25 ( + ) T cells that expresses CD45RO , histocompatibility leukocyte antigen DR , and intracellular cytotoxic T lymphocyte associated antigen ( CTLA ) 4 does not expand after stimulation and markedly suppresses the expansion of conventional T cells in a contact dependent manner . ^^^ After activation , CD 4 ( + ) CD 25 ( + ) T cells express CTLA 4 on the surface detectable for several weeks . ^^^ The anergic state of CD 4 ( + ) CD 25 ( + ) T cells is not reversible by the addition of anti CD 28 , anti CTLA 4 , anti transforming growth factor beta , or anti IL 10 antibody . ^^^ However , the refractory state of CD 4 ( + ) CD 25 ( + ) T cells was partially reversible by the addition of IL 2 or IL 4 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Human cd 25 ( + ) cd 4 ( + ) t regulatory cells suppress naive and memory T cell proliferation and can be expanded in vitro without loss of function . ^^^ Mouse CD 4 ( + ) Tr cells that express CD 25 possess remarkable suppressive activity in vitro and in autoimmune disease models in vivo . ^^^ Thus far , the existence of a similar subset of CD 25 ( + ) CD 4 ( + ) Tr cells in humans has not been reported . ^^^ Here we show that human CD 25 ( + ) CD 4 ( + ) Tr cells isolated from peripheral blood failed to proliferate and displayed reduced expression of CD 40 ligand ( CD40L ) , in response to T cell receptor mediated polyclonal activation , but strongly upregulated cytotoxic T lymphocyte associated antigen ( CTLA ) 4 . ^^^ Human CD 25 ( + ) CD 4 ( + ) Tr cells also did not proliferate in response to allogeneic antigen presenting cells , but they produced interleukin ( IL ) 10 , transforming growth factor ( TGF ) beta , low levels of interferon ( IFN ) gamma , and no IL 4 or IL 2 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Ex vivo isolation and characterization of CD 4 ( + ) CD 25 ( + ) T cells with regulatory properties from human blood . ^^^ It has been known for years that rodents harbor a unique population of CD 4 ( + ) CD 25 ( + ) `` professional ' ' regulatory / suppressor T cells that is crucial for the prevention of spontaneous autoimmune diseases . ^^^ Here we demonstrate that CD 4 ( + ) CD 25 ( + ) CD45RO ( + ) T cells ( mean 6 % of CD 4 ( + ) T cells ) are present in the blood of adult healthy volunteers . ^^^ In contrast to previous reports , these CD 4 ( + ) CD 25 ( + ) T cells do not constitute conventional memory cells but rather regulatory cells exhibiting properties identical to their rodent counterparts . ^^^ Cytotoxic T lymphocyte associated antigen ( CTLA ) 4 ( CD 152 ) , for example , which is essential for the in vivo suppressive activity of CD 4 ( + ) CD 25 ( + ) T cells , was constitutively expressed , and remained strongly upregulated after stimulation . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) immune regulatory cells are required for induction of tolerance to alloantigen via costimulatory blockade . ^^^ Immune regulatory CD 4 ( + ) CD 25 ( + ) cells play a vital role in the induction and maintenance of self tolerance and are essential for T cell homeostasis and the prevention of autoimmunity . ^^^ To determine whether CD 4 ( + ) CD 25 ( + ) cells regulate T cell responses to alloantigen and are critical for tolerance induction , murine CD 4 ( + ) T cells were tolerized to alloantigen via ex vivo CD 40 ligand ( CD40L ) / CD40 or CD28 / cytotoxic T lymphocyte associated antigen 4 / B7 blockade resulting in secondary mixed leukocyte reaction hyporesponsiveness and tolerance to alloantigen in vivo . ^^^ CD 4 ( + ) CD 25 ( + ) T cells were found to be potent regulators of alloresponses . ^^^ Depletion of CD 4 ( + ) CD 25 ( + ) T cells from the CD 4 ( + ) responder population completely abrogated ex vivo tolerance induction to alloantigen as measured by intact responses to alloantigen restimulation in vitro and in vivo . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The lungs of memory immune mice contained CD 4 ( + ) and CD 8 ( + ) T cells with the cell surface phenotype characteristic of memory cells ( CD 69 ( low ) CD 25 ( low ) CD 44 ( high ) ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CT but not LT IIa or LT IIb reduced the expression of CD 4 ( + ) T cell surface activation markers ( CD 25 and CD 69 ) and subsequent proliferative responses of anti CD 3 stimulated T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
After 3 days , the expression of CD 25 and CD8alpha in mitogen stimulated CD 4 ( + ) cells increased 10 to 20 fold as measured by flow cytometry . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Regulatory CD 4 ( + ) CD 25 ( + ) T cells in tumors from patients with early stage non small cell lung cancer and late stage ovarian cancer . ^^^ However , increased percentages of CD 4 ( + ) CD 25 ( + ) T cells were observed in the non small cell lung cancer tumor infiltrating lymphocytes and ovarian cancer tumor associated lymphocytes . ^^^ Furthermore , these CD 4 ( + ) CD 25 ( + ) T cells were found to secrete transforming growth factor beta , consistent with the phenotype of regulatory T cells . ^^^ Thus , tumor associated T cells from patients with early and late stage epithelial tumors contain increased proportions of CD 4 ( + ) CD 25 ( + ) T cells that secrete the immunosuppressive cytokine transforming growth factor beta . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
METHODS : The surface expression of VLA 4 , Mac 1 , ICAM 1 , CD 4 , CD 25 , CD 69 , CD 89 , IL 5 receptor and GM CSF receptor on eosinophils isolated from the peripheral blood of 15 patients with eosinophilia and 16 healthy volunteers was measured . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Immunoregulatory CD 25 ( + ) CD 4 T cells are thought to arise from the thymus as a distinct lineage of CD 4 T cells specific for self Ags . ^^^ Ag dose or using an oral tolerance protocol yielded the greatest numbers of Ag specific CD 25 ( + ) CD 4 T cells . ^^^ In contrast , immunogenic Ag exposure in the presence of an adjuvant did not lead to emergence of CD 25 ( + ) CD 4 T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The analytical method included the evaluation of T cell subpopulations ( using AcMc CD 2 , CD 4 , CD 8 ) and activated T cells ( AcMc HLA DR and CD 25 ) from CSF and peripheral blood by immunocytochemical techniques on a fixed cell slide as described by Bernd . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
IL 2 / Ig treatment of healthy rhesus monkeys induced significant increases in CD 4 ( + ) T lymphocyte counts and expression of CD 25 by these cells . ^^^ Short courses of IL 2 / Ig treatment of simian immunodeficiency virus ( SIV ) infected rhesus monkeys in conjunction with antiretroviral drugs resulted in increased CD 25 expression on T lymphocytes , and transient increases in CD 4 ( + ) T lymphocyte counts . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) regulatory T cells inhibit organ specific autoimmune diseases induced by CD 4 ( + ) CD 25 ( ) T cells and are potent suppressors of CD 4 ( + ) CD 25 ( ) T cell activation in vitro . ^^^ We demonstrate that CD 4 ( + ) CD 25 ( + ) T cells also suppress both proliferation and IFN gamma production by CD 8 ( + ) T cells induced either by polyclonal or Ag specific stimuli . ^^^ CD 4 ( + ) CD 25 ( + ) T cells inhibit the activation of CD 8 ( + ) responders by inhibiting both IL 2 production and up regulation of IL 2Ralpha chain ( CD 25 ) expression . ^^^ Suppression is mediated via a T T interaction as activated CD 4 ( + ) CD 25 ( + ) T cells suppress the responses of TCR transgenic CD 8 ( + ) T cells stimulated with soluble peptide MHC class 1 tetramers in the complete absence of APC . ^^^ These results broaden the immunoregulatory role played by CD 4 ( + ) CD 25 ( + ) T cells in the prevention of autoimmune diseases , but also raise the possibility that they may hinder the induction of effector CD 8 ( + ) T cells to tumor or foreign Ags . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Here , we report the identification of a CD 4 ( + ) population of regulatory T cells in the circulation of humans expressing high levels of CD 25 that exhibit in vitro characteristics identical with those of the CD 4 ( + ) CD 25 ( + ) regulatory cells isolated in mice . ^^^ With TCR cross linking , CD 4 ( + ) CD 25 ( high ) cells did not proliferate but instead totally inhibited proliferation and cytokine secretion by activated CD 4 ( + ) CD 25 ( ) responder T cells in a contact dependent manner . ^^^ The CD 4 ( + ) CD 25 ( high ) regulatory T cells expressed high levels of CD45RO but not CD45RA , akin to the expression of CD45RB ( low ) on murine CD 4 ( + ) CD 25 ( + ) regulatory cells . ^^^ Increasing the strength of signal by providing either costimulation with CD 28 cross linking or the addition of IL 2 to a maximal anti CD 3 stimulus resulted in a modest induction of proliferation and the loss of observable suppression in cocultures of CD 4 ( + ) CD 25 ( high ) regulatory cells and CD 4 ( + ) CD 25 ( ) responder cells . ^^^ Whereas higher ratios of CD 4 ( + ) CD 25 ( high ) T cells are required to suppress proliferation if the PD L 1 receptor is blocked , regulatory cell function is shown to persist in the absence of the PD 1 / PD L 1 or CTLA 4 / B7 pathway . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Of note , CD 25 ( + ) CD 4 ( + ) cells from CD 28 ( / ) mice similar to those from the wild type mice were efficient to prevent intestinal mucosal inflammation induced by the wild type CD45RB ( high ) cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Activated DR 6 ( / ) CD 4 ( + ) T cells exhibited upregulated CD 25 expression and enhanced proliferation in response to exogenous IL 2 stimulation . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
MEASUREMENTS : Analysis of peripheral blood on admission and 48 h later of the absolute white cell count and the immunophenotype of lymphocyte ( CD 3 , CD 3 HLADR , CD 4 , CD 8 , CD4 / CD8 ratio , CD 19 , and CD 25 ) and monocyte ( CD 13 , CD 13 HLADR , CD 14 , CD 14 HLADR , CD 13 CD14 , and CD 4 ) subpopulations . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The immunological mechanisms involved in this antitumor effect were suggested by a decisively increased secretion of tumor necrosis factor alpha TNFTnTNa and IFN gamma from CD 4 ( + ) and CD 8 ( + ) T cells and a markedly up regulated expression on CD 8 ( + ) T cells of high affinity IL 2 receptor alpha chain ( CD 25 ) , costimulatory molecule CD 28 , and adhesion molecule lymphocyte function associated antigen 2 ( LFA 2 / CD2 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
By flow cytometry in peripheral blood , pre and postinfusion assessment was made of : a ) CD 4 and CD 8 counts and mean fluorescence ; b ) CD 25 , human leukocyte antigen DR ( HLA DR ) , CD45RO and CD45RA expression on CD4+ T cells ; and c ) interferon ( IFN ) gamma , interleukin ( IL ) 4 and IL 5 expression in CD4+ T cells . ^^^ Following infusion in C0 . 5 , C1 . 5 and C3 . 0 but not Cpl : 1 ) the CD 4 , but not CD 8 count was significantly decreased ; 2 ) there was total loss of Leu3a staining ; 3 ) there were significant reductions in the mean fluorescence of OKT 4 binding ; and 4 ) there were significant reductions in the numbers of CD 25 , HLA DR , CD45RO and CD45RA / CD4+ cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Assembly of T cell receptor ( TCR ) alpha / beta genes by variable / diversity / joining ( 5 [ D ] J ) rearrangement is an ordered process beginning with recombination activating gene ( RAG ) expression and TCRbeta recombination in CD 4 ( ) CD 8 ( ) CD 25 ( + ) thymocytes . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Blood samples taken 24 h preoperatively from 49 patients ( 3 18 years old ) were stained with monoclonal antibodies for adhesion molecules ( ICAM 1 , LFA 1 , Mac 1 ) or constitutive / activation markers ( CD 4 , CD 14 , CD 16 , CD 25 , CD 54 , CD 69 , HLA DR ) and measured on a microbead calibrated FCM . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We investigated CD 25 , CD 4 , CD 8 expression and CD4 / CD8 ratio of systemic and peritoneal T lymphocytes in a rat model . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Role of CD 4 ( + ) CD 25 ( + ) regulatory T cells in T helper 2 cell mediated allergic inflammation in the airways . ^^^ It has recently been shown that CD 4 ( + ) CD 25 ( + ) T cells are immunoregulatory T cells that prevent CD 4 ( + ) T cell mediated organ specific autoimmune diseases . ^^^ To determine whether CD 4 ( + ) CD 25 ( + ) T cells downregulate Th 2 cell mediated allergic inflammation in the airways , we studied antigen induced eosinophil recruitment in the airways in BALB / c Rag 2 ( ) ( / ) mice transferred with CD 4 ( + ) CD 25 ( + ) T cell depleted or unfractionated T cells from ovalbumin specific TCR transgenic mice . ^^^ Antigen induced eosinophil recruitment into the airways was significantly decreased in the mice transferred with CD 4 ( + ) CD 25 ( + ) T cell depleted splenocytes as compared with those transferred with unfractionated splenocytes . ^^^ On the other hand , the depletion of CD 4 ( + ) CD 25 ( + ) T cells increased antigen induced neutrophil and T cell recruitment in the airways of the mice . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Cell contact dependent immunosuppression by CD 4 ( + ) CD 25 ( + ) regulatory T cells is mediated by cell surface bound transforming growth factor beta . ^^^ CD 4 ( + ) CD 25 ( + ) T cells have been identified as a population of immunoregulatory T cells , which mediate suppression of CD 4 ( + ) CD 25 ( ) T cells by cell cell contact and not secretion of suppressor cytokines . ^^^ In this study , we demonstrated that CD 4 ( + ) CD 25 ( + ) T cells do produce high levels of transforming growth factor ( TGF ) beta 1 and interleukin ( IL ) 10 compared with CD 4 ( + ) CD 25 ( ) T cells when stimulated by plate bound anti CD 3 and soluble anti CD 28 and / or IL 2 , and secretion of TGF beta 1 ( but not other cytokines ) , is further enhanced by costimulation via cytotoxic T lymphocyte associated antigen ( CTLA ) 4 . ^^^ As in prior studies , we found that CD 4 ( + ) CD 25 ( + ) T cells suppress proliferation of CD 4 ( + ) CD 25 ( ) T cells ; however , we observed here that such suppression is abolished by the presence of anti TGF beta . ^^^ In addition , we found that CD 4 ( + ) CD 25 ( + ) T cells suppress B cell immunoglobulin production and that anti TGF beta again abolishes such suppression . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
These comparisons suggest that only a minority of the TCR ( + ) CD8alpha alpha ( + ) intraepithelial lymphocytes ( IEL ) of the transgenic euthymic mice originate from hematopoietic precursors acquiring a TCR in the gut wall , while a majority of these CD8alpha alpha ( + ) IEL appear to be of thymic origin ( as were all TCR ( + ) CD8alpha beta ( + ) or CD 4 ( + ) in any location ) ; these last cells are released from the thymus as double negative thymocytes , which are at a more immature stage ( CD 44 ( + ) CD 25 ( + ) ) in female mice than in males ( CD 44 ( ) ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
There was also no change in the proportion of lymphocytes in the BAL expressing CD 3 , CD 4 , CD 8 , CD 19 , nor activation markers CD 25 or CD 69 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Most CD 4 cells in such cultures had the phenotype CD 25 ( ) CD 69 ( ) DR ( ) Ki 67 ( ) and were CD45RO ( bright ) CD45RA ( dim ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
RESULTS : Immunostaining of bronchial biopsy specimens showed no changes in the numbers of eosinophils , neutrophils , basophils , mast cells , CD 3 ( + ) , CD 4 ( + ) or CD 8 ( + ) T cells , CD 25 ( + ) cells or macrophages , or cells mRNA ( + ) for IL 4 , IL 5 , or IL 13 when the FC1P day was compared with the diluent control day . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Unique chemotactic response profile and specific expression of chemokine receptors CCR 4 and CCR 8 by CD 4 ( + ) CD 25 ( + ) regulatory T cells . ^^^ In rodents and humans , a unique subset of CD 4 ( + ) CD 25 ( + ) cytotoxic T lymphocyte antigen ( CTLA ) 4 ( + ) regulatory T cells ( Treg ) has been proposed to control peripheral tolerance . ^^^ Here , we determined the chemotactic response profile and chemokine receptor expression of human blood borne CD 4 ( + ) CD 25 ( + ) Treg cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Control of organ specific autoimmunity by immunoregulatory CD 4 ( + ) CD 25 ( + ) T cells . ^^^ CD 4 ( + ) CD 25 ( + ) T cells regulate the activity of autoreactive T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Natural regulatory CD 4 T cells expressing CD 25 . ^^^ In normal mice , a subpopulation of CD 4 T cells constitutively express CD 25 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Expression of HLA DR , CD 38 and CD 95 increased on both CD 4 and CD 8 T lymphocytes whereas CD 25 induction was observed exclusively on CD 4 T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Lymphocyte subpopulations were investigated using monoclonal antibodies ( Moscow ) to the following antigens : CD 3 ( T lymphocytes ) , CD 4 ( T helpers ) , CD 8 ( T supressors ) , CD 20 ( 8 lymphocytes ) , CD 25 ( IL 2 receptor ) , CD 16 ( natural killers ) , CD 95 ( activated cells ready to apoptosis ) . ^^^ In MS we found a fall of CD 3 , CD 4 , CD 8 , CD 20 and CD 16 , but an increase of CD4 / CD8 , CD 95 , CD 25 . ^^^ We found an increase of CD 3 , CD 4 , CD 16 , CD 25 , but a decrease of IL 1 ( p < 0 . 01 ) spontaneous production and IL 6 , IL 8 , TNF a stimulated secretion in DR 2 ( + ) MS patients , comparing to DR 2 ( ) patients and controls . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Activation of CD 25 ( + ) CD 4 ( + ) regulatory T cells by oral antigen administration . ^^^ CD 25 ( + ) CD 4 ( + ) T cells are naturally occurring regulatory T cells that are anergic and have suppressive properties . ^^^ We found that oral administration of OVA to OVA TCR transgenic mice resulted in a modification of the ratio of CD 25 ( + ) CD 4 ( + ) to CD 25 ( ) CD 4 ( + ) cells with an increase of CD 25 ( + ) CD 4 ( + ) T cells accompanied by a decrease of CD 25 ( ) CD 4 ( + ) T cells . ^^^ The relative increase in CD 25 ( + ) CD 4 ( + ) T cells persisted for as long as 4 wk post feeding . ^^^ We also found that CTLA 4 was dominantly expressed in CD 25 ( + ) CD 4 ( + ) T cells and there was an increase in the percentage of CD 25 ( + ) CD 4 ( + ) T cells expressing CTLA 4 in OVA fed mice . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) T cells facilitate the induction of T cell anergy . ^^^ A similar state of unresponsiveness is observed when the proliferative response of murine CD 4 ( + ) CD 25 ( ) T cells is suppressed in vitro by coactivated CD 4 ( + ) CD 25 ( + ) T cells . ^^^ We have developed a suppression system that uses beads coated with anti CD 3 and anti CD 28 Abs as surrogate APCs to study the interaction of CD 4 ( + ) CD 25 ( + ) and CD 4 ( + ) CD 25 ( ) T cells in vitro . ^^^ CD 4 ( + ) CD 25 ( + ) T cell induced suppression , in this model , was not abrogated by blocking the B 7 CTLA 4 pathway . ^^^ When the CD 4 ( + ) CD 25 ( ) T cells were separated from the CD 4 ( + ) CD 25 ( + ) suppressor cells after 24 h of coactivation by the Ab coated beads , the CD 4 ( + ) CD 25 ( ) T cells were unable to proliferate or to produce IL 2 upon restimulation . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The analysis of T cell subsets was performed with a flow cytometry method and with the following antibodies : anti CD 3 , CD 4 , CD 8 , CD 16 , CD 25 , CD 45 , CD 56 , and HLA DR . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
To obtain resting memory CD 4 T cells containing HIV 1 provirus , residual CD 25 ( + ) , CD 69 ( + ) and HLA DR ( + ) cells were immunodepleted after a 3 week cultivation period . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We propose that the malignant cell transformation of LBC cells could coincide with the transition stage from late double negative , DN 3 ( CD 4 CD 8 CD44 / low , CD25+ ) or DN 4 ( CD 4 low , CD 8 / low , CD 44 , CD 25 ) to double positive ( DP : CD4+CD8+ ) stage of T cell development . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
MAb to antigens CD 25 , CD 8 , CDw 50 , CD 5 , CD 4 , CD 7 , CD 3 , CD 71 , CD 34 , CD 45 , CD 38 , CD11b , CD 16 , and CD 95 have gained worldwide recognition at International HDLA Workshop Conferences . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Major histocompatibility complex ( MHC ) class 2 expressing cells as well as CD 3 , CD 4 , CD 25 , CD 28 , CD 68 , CD 80 , and CD 83 positive cells were localized immunohistologically . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The OLP lesions showed higher frequencies of subepithelial cells expressing CD1a , CD 86 , CD 4 , CD 8 and CD 25 than the cGVHD lesions . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
This unusually high IgE response was prevented by the infusion of regulatory alpha / beta CD 4 ( + ) T cells belonging to both CD 25 ( + ) and CD 25 ( ) subpopulations . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
After T cell transfer in lymphopenic recipients , the present results show that alloreactive T cells rapidly divided ; up regulated CD 69 , CD 25 , and CD 4 molecules ; and down regulated CD62L . ^^^ In contrast , nonalloreactive T cells started to divide later and did not up regulate CD 69 , CD 25 , and CD 4 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Using DO11 . 10 TCR transgenic T cells , we confirmed that CD 28 expression was dispensable for homeostatic expansion , and showed that the presence of endogenous CD 4 ( + ) CD 25 ( + ) regulatory cells did not detectably influence homeostatic expansion . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
After collecting blood samples from all rats , the CD 3 + ( T cells ) , CD 4 + ( T helper ) , CD 8 + ( T cytotoxic ) , CD 19 + ( B cells ) and CD 25 + ( IL 2 receptor , active T cell ) cell ratios were determined by indirect immunofluorescence method and , serum IgG , IgA , IgM levels were evaluated by using rat radial immunodiffusion plates . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Lymphocyte subset markers ( CD 4 , CD 8 , CD45RA , CD62L , CD 16 , CD 19 ) , activation markers ( HLA DR , CD 38 , CD 25 ) were performed by cytometry analysis . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The surface phenotypes of activated PBMC or PBMC were tested by two color immunostaining technique with anti CD 3 , CD 4 , CD 8 and also anti CD 16 , CD 25 or CD 56 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Tumor process involved enhanced expression of the activation markers CD 25 , CD 71 and HLA DR as well as differentiation antigens CD 4 , CD 8 , CD 22 and CD 16 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
These cells , which express CD 4 , CD 25 , and CD62L , develop in the thymus and survive in a CD 28 dependent manner in the periphery to maintain the homeostatic equilibrium of immunity and tolerance . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
RESULTS : Increases in the proportions of total , helper ( CD 4 ( + ) ) , and activated ( CD 25 ( + ) ) T lymphocytes and natural killer cells were measured in the subjects ' blood after consumption of B . lactis HN 019 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
An arthritogenic subpopulation of CD 4 ( + ) T cells , enriched by either negative or positive selection , expressed the activation markers CD 25 ( IL 2 receptor alpha ) , CD 71 ( transferrin receptor ) , CD 134 ( OX 40 antigen ) and MHC class 2 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Homeostasis and anergy of CD 4 ( + ) CD 25 ( + ) suppressor T cells in vivo . ^^^ CD 4 ( + ) CD 25 ( + ) suppressor T ( TS ) cells play a critical role in the maintenance of peripheral tolerance . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Age associated thymic atrophy is not associated with a deficiency in the CD 44 ( + ) CD 25 ( ) CD 3 ( ) CD 4 ( ) CD 8 ( ) thymocyte population . ^^^ Age associated thymic atrophy has been proposed to be due to changes in both the thymic microenvironment and in the intrinsic properties of the early T cell progenitors , the CD 44 ( + ) CD 25 ( ) CD 3 ( ) CD 4 ( ) CD 8 ( ) cells . ^^^ We also demonstrate that in the presence of anti IL 7 , CD 44 ( + ) CD 25 ( ) CD 3 ( ) CD 4 ( ) CD 8 ( ) cells from young mice show reduced thymocyte development in fetal thymic organ cultures compared with controls . ^^^ The increased thymopoiesis seen in the old animals occurs in the sequential manner which would be anticipated for an agent working directly on the early stages , including the CD 44 ( + ) CD 25 ( ) CD 3 ( ) CD 4 ( ) CD 8 ( ) cells . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Depletion of CD 25 ( + ) CD 4 ( + ) T cells and treatment with tyrosinase related protein 2 transduced dendritic cells enhance the interferon alpha induced , CD 8 ( + ) T cell dependent immune defense of B 16 melanoma . ^^^ Importantly , depletion of regulatory CD 25 ( + ) CD 4 ( + ) T cells prevented growth of B 16 IFN alpha in most mice and promoted long lasting protective tumor immunity . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The CD 28 ( ) CD 4 ( + ) T cells did not express detectable levels of CD 25 , CD 69 , 5 ( alpha ) 24 , or CTLA 4 but expressed heterogeneous amounts of CD 45 RA on the surface . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The CD 4 ( + ) CD 25 ( + ) regulatory T cells can be found in the thymus , but their need to undergo positive and negative selection has been questioned . ^^^ Instead , it has been hypothesized that CD 4 ( + ) CD 25 ( + ) cells mature following TCR binding to MHC backbone , to low abundant MHC / peptide complexes , or to class 2 MHC loaded with peripheral autoantigens . ^^^ In all these circumstances , processes that are distinct from positive and negative selection would govern the provenance of CD 4 ( + ) CD 25 ( + ) cells in the thymus . ^^^ By comparing the development of CD 4 ( + ) CD 25 ( ) and CD 4 ( + ) CD 25 ( + ) cells in mice expressing class 2 MHC molecules bound with one or many peptide ( s ) , we show that the CD 4 ( + ) CD 25 ( + ) cells appear during natural selection of CD 4 ( + ) T cells . ^^^ The proportion of CD 4 ( + ) CD 25 ( + ) cells in the population of CD 4 ( + ) thymocytes remains constant , and their total number reflects the complexity of selecting class 2 MHC / peptide complexes . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) T cell development is regulated by at least 2 distinct mechanisms . ^^^ It has recently been shown that CD 4 ( + ) CD 25 ( + ) T cells are immunoregulatory T cells that prevent CD 4 ( + ) T cell mediated organ specific autoimmune diseases . ^^^ In this study , the regulatory mechanism of CD 4 ( + ) CD 25 ( + ) T cell development were investigated using T cell receptor ( TCR ) transgenic mice . ^^^ It was found that CD 4 ( + ) CD 25 ( + ) T cells preferentially expressed the endogenous TCRalpha chain in DO 10 ( + ) TCR transgenic mice compared with CD 4 ( + ) CD 25 ( ) T cells . ^^^ Finally , it was found that , in contrast to thymus , CD 4 ( + ) CD 25 ( + ) T cells were readily detected in spleen of DO 10 ( + ) TCR alpha ( / ) mice in positively selecting backgrounds and that splenic CD 4 ( + ) CD 25 ( + ) T cells , but not CD 4 ( + ) CD 25 ( + ) thymocytes , were significantly decreased in B cell deficient mice , suggesting that B cells may control the peripheral pool of CD 4 ( + ) CD 25 ( + ) T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We also report low numbers of resting CD 4 ( + ) CD 25 ( + ) T cells in IMD patients , a subset of T cells shown to have important immunoregulatory functions in abrogating autoimmunities in 3 day thymectomized experimental mice . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In vivo migration of T cells was assessed by adoptive transfer of ( 51 ) Cr labelled CD 4 ( + ) CD 25 ( ) alpha beta ( + ) T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The MZ 93 cell line , established from a patient with CML , expressed CD 4 , CD 7 , CD 13 , CD 25 , CD 33 , CD 34 , CD 56 and NKp 46 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In addition , in CD 2 / animals , lymph node CD 4 SP T cells manifest a 10 to 100 fold attenuated activation response to cytochrome c ( CytC ) agonist peptides as judged by induction of CD 25 and CD 69 cell surface expression or [ ( 3 ) H ] TdR incorporation ; differences in the magnitude of responsiveness and requisite molar peptide concentrations were even greater for altered peptide ligands . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Mice that inherit the viable hypomorphic 17Rn5 ( 1989SB ) eed allele sustain a partial developmental block at or before the CD 4 ( ) CD 8 ( ) CD 44 ( ) CD 25 ( + ) stage of thymocyte differentiation . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The peripheral blood lymphocytes included CD 4 , CD 8 , CD 25 and HLA DR . ^^^ The sputum lymphocytes and eosinophils included CD 4 , CD 8 , CD 25 and HLA DR , and EG 2 , respectively . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In contrast , expression of other surface markers , CD 25 and CD 4 for T cells and CD 19 and surface IgM for B cells , was not affected by NAC . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Preferential recognition of self antigens despite normal thymic deletion of CD 4 ( + ) CD 25 ( + ) regulatory T cells . ^^^ Some autospecific T cells nevertheless migrate to peripheral lymphoid organs but are kept under control by the recently identified CD 4 ( + ) CD 25 ( + ) regulatory T cell subset . ^^^ In this study we show that significantly more peripheral CD 4 ( + ) CD 25 ( + ) regulatory T cells recognize self than non self Ags . ^^^ However , we also show for a large panel of endogenous superantigens as well as for self peptide / MHC complexes that autospecific CD 4 ( + ) CD 25 ( + ) thymocyte precursors are normally deleted during ontogeny . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Peripheral blood mononuclear cells were cultured in vitro with live BRSV and analyzed by dual color flow cytometry for surface expression of CD 25 on CD 4 ( + ) , CD 8 ( + ) , and gammadeltaT cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
OBJECTIVES : To measure the percentages of peripheral blood CD 4 and CD 8 T cells expressing nave / memory ( CD45RA / CD45RO ) and activation ( HLA DR , CD 25 ) markers , as well as mRNA encoding interleukin 4 ( IL 4 ) and interleukin 5 ( IL 5 ) in atopic and nonatopic childhood asthmatics and in nonasthmatic controls matched for age and atopic status ; and to study the effects of inhaled glucocorticoid therapy of the asthmatics on these measurements . ^^^ The percentages of both CD 4 and CD 8 T lymphocytes expressing HLA DR and CD 25 were elevated in the asthmatics as compared with controls , and significantly reduced in association with de novo or augmented inhaled glucocorticoid therapy . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 , CD 8 , CD 25 and HLA DR were analyzed , with flow cytometry , in lymphocytes prepared from peripheral blood of uremic ( n = 10 ) and hemodialysis patients ( n = 10 ) . ^^^ In CAPD patients the relative distribution of CD 4 positive cells ( p < 0 . 001 ) was lower , while quantitative CD 25 level ( p < 0 . 01 ) and the relative count of HLA DR ( p < 0 . 0001 ) was increased in the peritoneal dialysate compared to blood . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Monocytes and lymphocytes of 30 patients with acute hearing loss were determined by flow cytometry before , throughout and after HBO therapy using monoclonal antibodies to CD 3 , CD 4 , CD 8 , CD 14 , CD 25 , CD 45 and HLA DR . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The studied antigens were the following : CD 3 , CD 4 , CD 8 , CD 14 , CD 19 , CD 25 , CD 38 , CD 45 , CD45RO , CD 54 , CD 56 , CD62L , CD 71 and HLA DR . ^^^ In our investigation the occurrence of chromosome aberrations negatively correlated with CD 25 ( IL 2R ) expression in both CD 4 and CD 8 positive T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
As changes of peripheral blood lymphocyte subsets ( LS ) may accompany severe systemic lymphocyte immune responses , we compared peripheral LS of 44 patients with CF , 23 non CF patients with recurrent pulmonary infections and 83 healthy controls ( flow cytometry ; CD 3 , CD 19 , CD 16 , CD 56 , CD 4 , CD 8 , CD11b , CD45RA , CD45RO , HLA DR and CD 25 antigens ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Medial synovium was immunostained using antibodies to anti type 2 collagen , CD 68 , CD 2 , CD 4 , CD 8 , CD 15 , CD 19 , CD 25 , HLA DR , CD1a and LN 5 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Pancreatic lymph node derived CD 4 ( + ) CD 25 ( + ) Treg cells : highly potent regulators of diabetes that require TRANCE RANK signals . ^^^ Here we show in a CD 8 ( + ) T cell mediated model of type 1 diabetes that CD 4 ( + ) CD 25 ( + ) Treg cells prevent beta cell destruction following localized inflammation in the islets of Langerhans . ^^^ Indeed , blockade of this pathway results in decreased frequency of CD 4 ( + ) CD 25 ( + ) Treg cells in the PLN , resulting in intra islet differentiation of CD 8 ( + ) T cells into CTLs and rapid progression to diabetes . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The combination of all four agonists significantly induced expression of CD 45 RO ( fresh p < 0 . 05 , and thawed p < 0 . 001 ) in both the CD 4 ( + ) and CD 8 ( + ) T cells expressing CD 25 ( fresh UCB p < 0 . 01 [ CD 4 ] and p < 0 . 005 [ CD 8 ] , respectively ; thawed UCB p < 0 . 001 [ CD 4 ] and p < 0 . 001 [ CD 8 ] ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
However , thymocyte development , cell cycle , and survival were unperturbed by mNumb overexpression , even though transgenic Numb was expressed at an early stage in thymocyte development ( CD 4 ( ) CD 8 ( ) CD 3 ( ) cells that were CD 44 ( + ) CD 25 ( + ) or CD 44 ( ) CD 25 ( + ) ; double negative 2 / 3 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The immunoprofile of L 82 corresponds to that seen typically in primary ALCL cells , with positivity for CD 30 , EMA , CD 3 , CD 4 , CD 25 , CD 71 , TIA 1 , and granzyme B ; the cells were negative for EBV related antigens . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We have previously reported that immunization of mice with melanoma cells transfected to secrete the superantigen , Staphylococcal enterotoxin A ( SEA ) , increased the production of antibodies to the B 700 melanoma antigen , stimulated the production of endogenous interleukin 2 ( IL 2 ) , activated the expression of CD 4 , CD 8 and CD 25 T cell markers and enhanced NK cell activity . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The earliest intrathymic precursors were CD 4 ( lo ) CD 8 ( ) CD 25 ( ) CD 44 ( + ) c Kit ( + ) cells that expressed elevated levels of Notch 1 mRNA . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
This approach can be consolidated by performing lymphocyte activation tests in parallel and measuring in vitro T cell activation by Candida albicans antigens by three colour flow cytometry based on CD 3 , CD 4 and either CD 69 or CD 25 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
RESULTS : There were significant increases in the numbers of intradermal CD 3 ( + ) ( P = . 007 ) , CD 4 ( + ) ( P = . 004 ) , CD 8 ( + ) ( P = . 012 ) , and CD 25 ( + ) ( P = . 018 ) T cells , as well as eosinophils ( P = . 02 ) , neutrophils ( P = . 01 ) , basophils ( P = . 004 ) , and macro phages ( P = . 0014 ) in patients with CIU compared with numbers in nonatopic control subjects . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In all cases tested , tumor cells were immunoreactive for CD 3 , CD 4 , CD 5 , and CD 25 and were nonimmunoreactive for CD 7 and CD 8 . ^^^ The tumor cells in all cases tested were positive for CD 2 , CD 3 , CD 4 , CD 5 , and CD 25 and were negative for CD 7 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Although the observations are converging towards the naturally activated CD 25 ( + ) CD 4 T cells as a major population responsible for this protection , there is still considerable disagreement on the molecular and cellular requirements involved to achieve a stable immune homeostasis in vivo . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The number of Gd enhanced lesions was significantly correlated with CSF cell counts , as well as the number of CD 4 ( + ) CD 29 ( + ) helper inducer and IL 2 receptor ( CD 25 ) positive activated helper T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Histological and FACS analysis of tumor infiltrating leukocytes reveal that the injection of recombinant virus expressing antibodies specific for the TCR complex attracts and activates ( CD 25 ( + ) , CD 69 ( + ) ) CD 4 and CD 8 lymphocytes . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
IL 10 was produced by a subpopulation of CD 4 T cells , which expressed the CD 25 component of the IL 2 receptor and the CTLA 4 receptor characteristic of regulatory T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Thus far , the potential role of CD 4 ( + ) CD 25 ( + ) T cells in human tumors has not been reported . ^^^ Furthermore , the CD 4 ( + ) CD 25 ( + ) T cells mediate potent inhibition of autologous T cell proliferation . ^^^ Together these results suggest that the CD 4 ( + ) CD 25 ( + ) T cells found in lung tumors selectively inhibit the host immune response and therefore could contribute to the progression of lung cancer . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Generation of CD 4 ( + ) CD 25 ( + ) regulatory T cells from autoreactive T cells simultaneously with their negative selection in the thymus and from nonautoreactive T cells by endogenous TCR expression . ^^^ One recent study demonstrated that CD 4 ( + ) CD 25 ( + ) T cells were generated from autoreactive T cells without negative selection . ^^^ To investigate the ontogeny of CD 4 ( + ) CD 25 ( + ) regulatory T cells , neo autoantigen bearing transgenic mice expressing chicken egg OVA systemically in the nuclei ( Ld nOVA ) were crossed with transgenic mice expressing an OVA specific TCR ( DO11 . 10 ) . ^^^ Ld nOVA 10 DO11 . 10 mice had increased numbers of CD 4 ( + ) CD 25 ( + ) regulatory T cells in the thymus and the periphery despite clonal deletion . ^^^ In Ld nOVA 10 DO11 . 10 mice , T cells expressing endogenous TCR alpha beta chains were CD 4 ( + ) CD 25 ( ) T cells , whereas T cells expressing autoreactive TCR were selected as CD 4 ( + ) CD 25 ( + ) T cells , which were exclusively dominant in recombination activating gene 2 deficient Ld nOVA 10 DO11 . 10 mice . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Surface expression of CD 25 , CD 28 , CD 40 , CD 54 , CD 80 , CD 86 and CTLA 4 was evaluated on CD 3 , CD 4 , CD 8 , CD 14 and CD 19 PBMC subpopulations by three colour flow cytometry . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Arrest of disease progression is accompanied by an enhanced frequency in the pancreatic lymph nodes of CD 4 ( + ) CD 25 ( + ) regulatory T cells that are able to inhibit the T cell response to the pancreatic autoantigen insulinoma associated protein 2 and to significantly delay disease transfer by pathogenic CD 4 ( + ) CD 25 ( ) cells . ^^^ Thus , a short treatment of adult NOD mice with an analog of 1 , 25 dihydroxyvitamin D ( 3 ) inhibits IL 12 production , blocks pancreatic infiltration of Th 1 cells , enhances CD 4 ( + ) CD 25 ( + ) regulatory cells , and arrests the progression of type 1 diabetes , suggesting its possible application in the treatment of human autoimmune diabetes . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
T cell activation defined in flow cytometry by CD 4 ( high ) and / or CD 25 markers were observed in all type 1 diabetic patients and some at risk subjects , but not in normal control subjects . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Another set of evidence indicates that a subpopulation of CD 4 ( + ) T cells constitutively expressing the CD 25 molecule prevent lymphopenia induced autoimmunity and inflammatory bowel disease . ^^^ We tested the ability of this CD 25 ( + ) CD 4 ( + ) population to regulate CD 4 ( + ) T cell mediated inflammatory response to PC . ^^^ Adoptive transfer of CD 25 ( ) CD 4 ( + ) cells into PC infected recombination activating gene 2 deficient mice led to lethal pneumonia within 13 days post transfer . ^^^ PC infection appeared to trigger CD 25 ( ) CD 4 ( + ) cells , since recipients with reduced PC load survived up to 5 weeks after transfer . ^^^ In contrast , transfer of CD 25 ( + ) CD 4 ( + ) cells did not induce lethal pneumonia and prevented the development of the disease induced by CD 25 ( ) CD 4 ( + ) cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Lymphocyte subgroups containing CD 4 , CD 5 , CD 6 , CD 8 , CD 19 , CD 23 , and CD 25 , and immunoglobulin G ( IgG ) , IgM , IgA , and IgE , were in the normal range ; hemoglobin F ( HbF ) , 2 . 8 % . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Both CD 4 ( + ) CD 25 ( + ) and CD 4 ( + ) CD 25 ( ) regulatory cells mediate dominant transplantation tolerance . ^^^ CD 4 ( + ) CD 25 ( + ) T cells have been proposed as the principal regulators of both self tolerance and transplantation tolerance . ^^^ Although CD 4 ( + ) CD 25 ( + ) T cells do have a suppressive role in transplantation tolerance , so do CD 4 ( + ) CD 25 ( ) T cells , although 10 fold less potent . ^^^ CD 4 ( + ) CD 25 ( + ) T cells from naive mice can also prevent rejection despite the lack of any previous experience of donor alloantigens . ^^^ Serial analysis of gene expression confirmed that cells sorted into CD 4 ( + ) CD 25 ( + ) and CD 4 ( + ) CD 25 ( ) populations were distinct in that they responded to TCR ligation with very different programs of gene expression . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Absence of transgenic CD 25 ( + ) 4 ( + ) cells in animals deficient for endogenous TCRalpha chains and analyses of endogenous TCR gene expression in subsets of CD 4 ( + ) cells from T / R ( + ) mice demonstrate that development of transgenic MBP specific CD 25 ( + ) 4 ( + ) T ( reg ) depends on the coexpression of endogenous TCRalpha chains . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Furthermore , we found higher expression of CD 25 molecule on T CD 3 ( + ) and T CD 4 ( + ) lymphocytes in the study group , when compared to controls . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The accumulation of CD 4 ( ) CD 8 ( ) stage of thymocytes in 22 month old BXD mice was further identified to be associated with a developmental block between the CD 25 ( ) CD 44 ( + ) and the CD 25 ( + ) CD 44 ( + ) stages . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) regulatory T cells prevent organ specific autoimmune diseases in various animal models . ^^^ Adult peripheral blood contained two populations of CD 4 ( + ) T cells that expressed CD 25 at different densities . ^^^ In addition to the activation / memory T cell antigens mentioned above they also expressed intracellular CD 152 ( CTLA 4 ) as well as enhanced levels of cell surface CD 122 , similar to the murine CD 4 ( + ) CD 25 ( + ) regulatory counterpart . ^^^ CD 25 ( ++ ) , CD 152 ( + ) and CD 122 ( ++ ) cells were present in paediatric thymus ( 10 % of CD 4 ( + ) CD 8 ( ) thymocytes ) expressing signs of recent selection ( CD69+ ) and in cord blood ( 5 % of CD 4 ( + ) cells ) where they showed a naive phenotype . ^^^ In addition , cord blood contained a small population of CD 25 ( + ) cells ( approximately 2 % of CD 4 T cells ) that were CD 152 ( ) and CD 122 ( low ) and displayed signs of activation . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Thymectomy of BALB / c mice on day 3 of life results in the development of autoimmune gastritis ( AIG ) due to the absence of CD 4 ( + ) CD 25 ( + ) regulatory T cells . ^^^ However , depletion of CD 4 ( + ) CD 25 ( + ) T cells by treatment with anti CD 25 rarely resulted in AIG . ^^^ One explanation for this result is that CD 4 ( + ) CD 25 ( ) T cells upon transfer to nude recipients undergo lymphopenia induced proliferation , providing a signal for T cell activation . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Parameters of cellular and humoral immunity ( differential blood count , T cell markers CD 2 , CD 3 , CD 4 , and CD 8 , B cell markers CD 19 and CD 20 , monocyte markers CD 13 and CD 14 , NK ( natural killer ) cell marker CD 16 , activation markers CD 25 , CD 26 , CD 69 , and HLA DR , and cytokines IL 1 receptor antagonist ( IL 1RA ) , IL 2 , soluble IL 2 receptor ( sIL 2R ) , IL 6 , IL 10 , and TGFbeta ) were measured in venous blood . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
ATPgammaS and BzATP inhibited secretion of IL 2 , IL 5 , IL 10 , and IFN gamma ; expression of CD 25 ; and proliferation after activation of CD 4 ( + ) T cells by immobilized anti CD 3 and soluble anti CD 28 Abs , without increasing cell death . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We found that aberrant expression on thymic stroma yielded predominantly CD 4 ( + ) CD 25 ( + ) T ( R ) cells , which under physiological conditions may be induced by ectopically expressed organ specific antigens and thus prevent organ specific autoimmunity . ^^^ Expression of the agonist antigen by nonactivated hematopoietic cells produced mostly CD 4 ( + ) CD 25 ( ) T ( R ) cells . ^^^ Suppression of CD 4 ( + ) T cell proliferative responses by both CD 25 ( + ) and CD 25 ( ) subsets was interleukin 10 ( IL 10 ) independent and was overcome by IL 2 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Results obtained revealed similar expression of CD 25 , CD 24 , CD 8 , CD 4 , C 18 and CD 44 , while differences in binding to hyaluronic acid ( HA ) were found . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
There was marked T cell activation in the lungs of SP D ( ) ( / ) mice , as reflected by an increased percentage of both CD 4 ( + ) and CD 8 ( + ) T cells expressing CD 69 and CD 25 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We have previously shown that regulatory CD 25 ( + ) CD 4 ( + ) T cells are resistant to clonal deletion induced by viral superantigen in vivo . ^^^ In this work we report that isolated CD 25 ( + ) CD 4 ( + ) T cells activated in vitro by anti CD 3 Ab are resistant to Fas induced apoptosis , in contrast to their CD 25 ( ) CD 4 ( + ) counterparts . ^^^ Resistance of CD 25 ( + ) CD 4 ( + ) T cells to Fas dependent activation induced cell death is not linked to their inability to produce IL 2 or to their ability to produce IL 10 . ^^^ The sensitivity of both populations to Fas induced apoptosis can be modulated in vitro by changing the CD 25 ( + ) CD 4 ( + ) : CD 25 ( ) CD 4 ( + ) T cell ratio . ^^^ The sensitivity of CD 25 ( ) CD 4 ( + ) T cells to apoptosis can be reduced , while the sensitivity of CD 25 ( + ) CD 4 ( + ) T cells can be enhanced . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) regulatory T cells can mediate suppressor function in the absence of transforming growth factor beta 1 production and responsiveness . ^^^ CD 4 ( + ) CD 25 ( + ) regulatory T cells inhibit organ specific autoimmune diseases induced by CD 4 ( + ) CD 25 ( ) T cells and are potent suppressors of T cell activation in vitro . ^^^ The role of TGF beta 1 in CD 4 ( + ) CD 25 ( + ) suppressor function remains unclear . ^^^ While most studies have failed to reverse suppression with anti transforming growth factor ( TGF ) beta 1 in vitro , one recent study has reported that CD 4 ( + ) CD 25 ( + ) T cells express cell surface TGF beta 1 and that suppression can be completely abrogated by high concentrations of anti TGF beta suggesting that cell associated TGF beta 1 was the primary effector of CD 4 ( + ) CD 25 ( + ) mediated suppression . ^^^ Here , we have reevaluated the role of TGF beta 1 in CD 4 ( + ) CD 25 ( + ) mediated suppression . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Human CD 4 ( + ) CD 25 ( + ) regulatory , contact dependent T cells induce interleukin 10 producing , contact independent type 1 like regulatory T cells [ corrected ] . ^^^ It has been recently demonstrated that regulatory CD 4 ( + ) CD 25 ( + ) CD45RO ( + ) T cells are present in the peripheral blood of healthy adults and exert regulatory function similar to their rodent counterparts . ^^^ Here we show that human CD 4 ( + ) CD 25 ( + ) T cells induce long lasting anergy and production of interleukin ( IL ) 10 in CD 4 ( + ) CD 25 ( ) T cells . ^^^ These anergized CD 4 ( + ) CD 25 ( ) T cells then suppress proliferation of syngenic CD 4 ( + ) T cells via IL 10 but independent of direct cell contact , similar to the so called type 1 regulatory T ( Tr 1 ) cells . ^^^ This ' catalytic ' function of CD 4 ( + ) CD 25 ( + ) T cells to induce Tr 1 like cells helps to explain their central role for the maintenance of immune homeostasis . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Infectious tolerance : human CD 25 ( + ) regulatory T cells convey suppressor activity to conventional CD 4 ( + ) T helper cells . ^^^ Regulatory CD 4 ( + ) CD 25 ( + ) T cells ( Treg ) are mandatory for maintaining immunologic self tolerance . ^^^ We demonstrate that the cell cell contact mediated suppression of conventional CD 4 ( + ) T cells by human CD 25 ( + ) Treg cells is fixation resistant , independent from membrane bound TGF beta but requires activation and protein synthesis of CD 25 ( + ) Treg cells . ^^^ Coactivation of CD 25 ( + ) Treg cells with Treg cell depleted CD 4 ( + ) T cells results in anergized CD 4 ( + ) T cells that in turn inhibit the activation of conventional , freshly isolated CD 4 ( + ) T helper ( Th ) cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In the adult , GR is specifically induced to high levels in CD 25 ( + ) CD 4 ( ) CD 8 ( ) thymocytes and returns to basal levels in CD 4 ( + ) CD 8 ( + ) thymocytes of wild type and positively selecting female HY TCR transgenic mice , but not negatively selecting male HY TCR transgenic mice . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Day 3 thymectomy ( D3Tx ) results in a loss of peripheral tolerance mediated by CD 4 ( + ) CD 25 ( + ) T cells and the development of autoimmune ovarian dysgenesis ( AOD ) in A / J and ( C57BL / 6J 10 A / J ) F ( 1 ) ( B6AF ( 1 ) ) hybrids but not in C57BL / 6J mice . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Utilizing flow cytometry , we found anti CD 3 activated CD 4 ( + ) T cells from these patients were incapable of fully upregulating activation markers ( CD 25 , CD 69 , and CD40L ) or producing interferon gamma and IL 2 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The expression of CD 25 and high levels of CD 44 was initiated in the thymus during the development from CD 4 ( + ) CD 8 ( + ) to CD 4 ( + ) ; a large proportion of maturing CD 4 ( + ) thymocytes expressed both CD 25 and high levels of CD 44 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Phenotype , localization , and mechanism of suppression of CD 4 ( + ) CD 25 ( + ) human thymocytes . ^^^ Phenotypic markers , localization , functional activities , and mechanisms of action in vitro of CD 4 ( + ) CD 25 ( + ) T cells , purified from postnatal human thymuses , were investigated . ^^^ These cells showed poor or no proliferation in mixed lymphocyte culture ( MLC ) , and suppressed in a dose dependent fashion the proliferative response to allogeneic stimulation of CD 4 ( + ) CD 25 ( ) thymocytes . ^^^ Virtually all CD 4 ( + ) CD 25 ( + ) thymocytes constitutively expressed cytoplasmic T lymphocyte antigen ( CTLA ) 4 , surface tumor necrosis factor type 2 receptor ( TNFR 2 ) , and CCR 8 . ^^^ After polyclonal activation , CD 4 ( + ) CD 25 ( + ) thymocytes did not produce the cytokines interleukin ( IL ) 2 , IL 4 , IL 5 , IL 13 , interferon gamma , and only a very few produced IL 10 , but all they expressed on their surface CTLA 4 and the majority of them also transforming growth factor ( TGF ) beta 1 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Donor type CD 4 ( + ) CD 25 ( + ) regulatory T cells suppress lethal acute graft versus host disease after allogeneic bone marrow transplantation . ^^^ CD 4 ( + ) CD 25 ( + ) regulatory T ( T ( reg ) ) cells have recently been shown to suppress proliferative responses of CD 4 ( + ) CD 25 ( ) T cells to alloantigenic stimulation in vitro and are required for ex vivo tolerization of donor T cells , which results in their reduced potential to induce aGVHD . ^^^ Here we show that CD 4 ( + ) CD 25 ( + ) T cells isolated from the spleen or BM of donor C57BL / 6 ( H 2 ( b ) ) mice that have not been tolerized are still potent inhibitors of the alloresponse in vitro and of lethal aGVHD induced by C57BL / 6 CD 4 ( + ) CD 25 ( ) T cells in irradiated BALB / c ( H 2 ( d ) ) hosts in vivo . ^^^ The addition of the CD 4 ( + ) CD 25 ( + ) T ( reg ) cells at a 1 : 1 ratio with responder / inducer CD 4 ( + ) CD 25 ( ) T cells resulted in a > 90 % inhibition of the mixed leukocyte reaction and marked protection from lethal GVHD . ^^^ This protective effect depended in part on the ability of the transferred CD 4 ( + ) CD 25 ( + ) T cells to secrete interleukin 10 and occurred if the T ( reg ) cells were of donor , but not host , origin . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) immunoregulatory T Cells : new therapeutics for graft versus host disease . ^^^ CD 4 ( + ) CD 25 ( + ) immunoregulatory T cells play a pivotal role in preventing organ specific autoimmune diseases and in tolerance induction to allogeneic organ transplants . ^^^ Here , we show that the few CD 4 ( + ) CD 25 ( + ) T cells naturally present in the transplant regulate GVHD because their removal from the graft dramatically accelerates this disease . ^^^ Furthermore , the addition of freshly isolated CD 4 ( + ) CD 25 ( + ) T cells at time of grafting significantly delays or even prevents GVHD . ^^^ Ex vivo expanded CD 4 ( + ) CD 25 ( + ) regulatory T cells obtained after stimulation by allogeneic recipient type antigen presenting cells can also modulate GVHD . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Stimulation of CD 25 ( + ) CD 4 ( + ) regulatory T cells through GITR breaks immunological self tolerance . ^^^ CD 25 ( + ) CD 4 ( + ) regulatory T cells in normal animals are engaged in the maintenance of immunological self tolerance . ^^^ We show here that glucocorticoid induced tumor necrosis factor receptor family related gene ( GITR , also known as TNFRSF 18 ) a member of the tumor necrosis factor nerve growth factor ( TNF NGF ) receptor gene superfamily is predominantly expressed on CD 25 ( + ) CD 4 ( + ) T cells and on CD 25 ( + ) CD 4 ( + ) CD 8 ( ) thymocytes in normal nave mice . ^^^ We found that stimulation of GITR abrogated CD 25 ( + ) CD 4 ( + ) T cell mediated suppression . ^^^ Thus , GITR plays a key role in dominant immunological self tolerance maintained by CD 25 ( + ) CD 4 ( + ) regulatory T cells and could be a suitable molecular target for preventing or treating autoimmune disease . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Rapamycin blocks the generation of regulatory T cells facilitated by posttransplant infusion of donor T cells through increasing the apoptosis of thymic CD 4 ( + ) CD 25 ( + ) cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Lymphocyte subpopulations were evaluated by enzyme immunoassay according to expression of membrane antigens CD 3 , CD 4 , CD 8 , CD 16 , CD 20 , CD 25 , CD 56 , CD 95 using monoclonal antibodies ICO ( Biomedspectr ) , humoral immunity by serum levels of IgA , IgM and IgG . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
There has recently been an explosion of renewed interest in regulatory T cells , particularly those within the CD 4 ( + ) CD 25 ( + ) population . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The disease protective WT CD 4 ( + ) cells are contained within the CD45RB ( low ) fraction and unexpectedly were found in both the CD 25 ( + ) and the CD 25 ( ) subpopulations of these cells , their frequency being higher in the latter . ^^^ The mechanism by which CD 25 ( + ) and CD 25 ( ) CD45RB ( low ) CD 4 ( + ) cells block colitis involves IL 10 and not transforming growth factor ( TGF ) beta , as treatment with anti IL 10R but not anti TGF beta monoclonal antibody abrogated their protective effect . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The number of lymphocytes expressing CD 3 , CD 4 , CD 8 , CD 16 , CD 25 , and CD 19 antigens was studied in patients with chronic hepatitis C before and after 12 week therapy with interferon alpha ( IFN alpha ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
By flow cytometry , the number of lymphocytes , granulocytes , epithelial cells and red blood cells was analysed as well as the expression on lymphocyte subsets of the following antigens : CD 3 , CD 4 , CD 8 , CD 19 , CD 25 , CD 38 , CD45RO , CD45RA , HLA DR , TcRgd , CD 161 and l selectin . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The enhanced responses to H / Kbeta in H / Kbeta deficient mice were shown to be intrinsic to CD 4 ( + ) CD 25 ( ) T cells rather than a change in status of CD 4 ( + ) CD 25 ( + ) regulatory T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Recently , CD 4 ( + ) CD 25 ( + ) T cells have been implicated in the control of diabetes , suggesting that the inflamed islets of Langerhans in prediabetic NOD mice are under peripheral immune surveillance . ^^^ Here we show that CD 4 ( + ) CD 25 ( + ) splenocytes inhibit diabetes in cotransfer with islet infiltrating cells . ^^^ Furthermore , CD62L expression is necessary for this disease delaying effect of CD 4 ( + ) CD 25 ( + ) cells in vivo , but not for their suppressor function in vitro . ^^^ We demonstrate that the CD 4 ( + ) CD 25 ( + ) CD62L ( + ) splenocytes express CCR 7 at high levels and migrate toward secondary lymphoid tissue chemokine and ELC ( macrophage inflammatory protein 3beta ) , lymphoid chemokines , whereas CD 4 ( + ) CD 25 ( + ) CD62L ( ) splenocytes preferentially express CCR 2 , CCR 4 , and CXCR 3 and migrate toward the corresponding inflammatory chemokines . ^^^ These data demonstrate that CD 4 ( + ) CD 25 ( + ) CD62L ( + ) , but not CD 4 ( + ) CD 25 ( + ) CD62L ( ) , splenocytes delay diabetes transfer , and that CD 4 ( + ) CD 25 ( + ) suppressor T cells are comprised of at least two subpopulations that behave differently in cotransfer in vivo and express distinct chemokine receptor and chemotactic response profiles despite demonstrating equivalent suppressor functions in vitro . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In this study , we measured the prevalence of T ( reg ) that coexpress CD 4 and CD 25 in the PBLs , tumor infiltrating lymphocytes , and regional lymph node lymphocytes from 65 patients with either pancreas or breast cancer . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Here , we show that CD 4 ( + ) CD 25 ( + ) regulatory T cells were not readily detected in IL 2Rbeta ( / ) mice , but the production of functional CD 4 ( + ) CD 25 ( + ) T cells was reconstituted in Tg / mice . ^^^ Adoptive transfer of normal CD 4 ( + ) CD 25 ( + ) T cells into neonatal IL 2Rbeta deficient mice prevented this lethal autoimmune syndrome . ^^^ The CD 4 ( + ) CD 25 ( + ) T cells in disease free adult IL 2Rbeta deficient recipient mice were present at a near normal frequency , were solely donor derived , and depended on IL 2 for expansion . ^^^ These observations indicate that the essential function of the IL 2 / IL 2R system primarily lies at the level of the production of CD 4 ( + ) CD 25 ( + ) regulatory T cells . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Increased CD 25 expression , especially in CD 4 ( + ) T cells but also in CD 8 ( + ) T cells , without increases in expression of the beta and gamma chains of the IL 2 receptor was detected in the IL 2 group . ^^^ Up to 79 % of naive CD 4 ( + ) T cells ( median , 61 % ) from patients in the IL 2 group expressed CD 25 , and the number of naive CD 4 ( + ) / CD25 ( + ) T cells correlated positively with both the total and naive CD 4 ( + ) T cell counts . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) T cell production in healthy humans and in patients with thymic hypoplasia . ^^^ Regulatory T cells are found primarily in the CD 4 ( + ) CD 25 ( + ) fraction of T cells and play an important role in the prevention of autoimmunity . ^^^ We examined CD 4 ( + ) CD 25 ( + ) T cells in 33 healthy children and adults and compared them to a population with an inherited form of thymic hypoplasia and a predisposition to autoimmune disease . ^^^ Absolute numbers of CD 4 ( + ) CD 25 ( + ) T cells were markedly higher in healthy infants than in infants with chromosome 22q11 . 2 deletion syndrome . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Furthermore , by enhancing CD 25 expression , extended signaling through surface replenishing TCRs significantly amplified the number of daughter cells generated by naive CD 4 ( + ) T cells that had already committed to proliferate . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
To assess the role of IL 2 in the generation and function of regulatory T cells , we transferred CD 4 ( + ) T cells from mice genetically deficient in IL 2 or IL 2R ( alpha ) ( CD 25 ) expression . ^^^ In contrast , splenic or thymic CD 4 ( + ) T cells from CD 25 knockout donor mice conferred little or no protection . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Patients ' peripheral blood mononuclear cells ( PBMC ) were phenotyped for CD 3 , CD 4 , CD 8 , and CD 25 by FACS analysis and tested for T cell proliferation in vitro in the presence of c MOv 18 with and without IL 2 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
However , in both groups expression of B7 . 2 on dendritic cells as well as CD 25 , CD 69 , and T1 / ST2 on CD 4 ( + ) T cells was enhanced , suggesting immune activation . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) regulatory T ( Treg ) cells naturally occur in mice and humans , and similar Treg cells can be induced in vivo and in vitro . ^^^ The suppressor cells developed from CD 4 ( + ) CD 25 ( ) CD45RO ( + ) cells . ^^^ The results suggest that 4C8 costimulation induces the generation of Treg cells that share phenotypic and functional features with CD 4 ( + ) CD 25 ( + ) T cells , and that CD 25 ( ) memory T cells may differentiate into certain Treg cell subsets in the periphery . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The T cell receptor expression was assessed by three colour flow cytometry using monoclonal antibodies against CD 3 , CD 4 , CD 8 , CD 25 and human leucocyte antigen ( HLA ) DR . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
However , significant amounts of CTLA 4 were observed in the CD 25 ( + ) CD 4 ( + ) subset of T cells which , when removed from the cultures , accounted for the CTLA 4 inhibition observed . ^^^ Overall , these data provide evidence that CD 80 and CD 86 differ in their interactions with CTLA 4 and that CD 80 appears to be the preferential inhibitory ligand for CTLA 4 working via a population of CD 4 ( + ) CD 25 ( + ) CTLA 4 ( + ) regulatory T cells . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Previously we reported that TGF beta has an important role in the generation and expansion of human `` professional ' ' CD 4 ( + ) CD 25 ( + ) regulatory T cells in the periphery that have a cytokine independent mechanism of action . ^^^ We report that TGF beta induces activated CD 4 ( + ) CD 25 ( ) T cells to become Th 3 suppressor cells . ^^^ While stimulating CD 4 ( + ) cells with TGF beta modestly increased expression of CD 25 and intracellular CTLA 4 in primary cultures , upon secondary stimulation without TGF beta the total number and those expressing these markers dramatically increased . ^^^ Depletion studies revealed that the precursors of these TGF beta producing CD 4 ( + ) suppressor cells were CD 25 negative . ^^^ These studies provide evidence that CD 4 ( + ) CD 25 ( + ) regulatory cells in human blood consist of at least two subsets that have TGF beta dependent and independent mechanisms of action . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Peripheral MHC 2 expression appeared to influence CD 4 ( + ) T cell reactivity by two complementary mechanisms : maintenance of CD 4 ( + ) CD 25 ( + ) regulatory T cells ( `` suppression ' ' ) and direct dampening of CD 4 ( + ) T cell reactivity ( `` tuning ' ' ) . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Here , we have studied the MHC class 2 molecule presentation of gastric parietal cell ( PC ) specific H ( + ) / K ( + ) ATPase , which induces a destructive autoimmune gastritis in BALB / c mice lacking CD 4 ( + ) CD 25 ( + ) regulatory T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The current study investigates the role of CD 4 ( + ) CD 25 ( + ) T regulatory ( T ( R ) ) cells during autoimmune disease using the CD 4 ( + ) T cell dependent myelin oligodendrocyte glycoprotein ( MOG ) specific experimental autoimmune encephalomyelitis model of multiple sclerosis . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Furthermore , in contrast to recently described CD 4 ( + ) CD 25 ( + ) T ( S ) and plasmacytoid dendritic cell activated T ( S ) , tumor induced L selectin ( high ) T ( S ) prevent tumor rejection via blockade of sensitized , activated T ( E ) rather than via afferent blockade . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In chimeras reconstituted with bone marrow cells from IL 2Ralpha deficient donors , restitution of a population of CD 25 ( + ) CD 4 ( + ) T cells prevents the chaotic accumulation of lymphoid cells , and rescues the mice from autoimmune disease and death . ^^^ The reintroduction of IL 2 producing cells in IL 2 deficient chimeras establishes a population of CD 25 ( + ) CD 4 ( + ) T cells , and restores the peripheral lymphoid compartments to normal . ^^^ The CD 25 ( + ) CD 4 ( + ) T cells regulated selectively the number of naive CD 4 ( + ) T cells transferred into T cell deficient hosts . ^^^ The CD 25 ( + ) CD 4 ( + ) / naive CD 4 T cell ratio and the sequence of cell transfer determines the homeostatic plateau of CD 4 ( + ) T cells . ^^^ Overall , our findings demonstrate that IL 2Ralpha is an absolute requirement for the development of the regulatory CD 25 ( + ) CD 4 ( + ) T cells that control peripheral CD 4 T cell homeostasis , while IL 2 is required for establishing a sizeable population of these cells in the peripheral pools . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
HDAC 1 furthermore inhibited activation induced CD 25 and CD 154 expression on CD 4 cells , without affecting induction of CD 69 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 3 , CD 4 , CD 8 , CD 25 , Mast Cell Tryptase monoclonal antibodies were used to characterise stroma inflammatory cells in nine cervical tumours . ^^^ The proportion of CD 3 ( + ) , CD 4 ( + ) , and CD 25 ( + ) cells was significantly lower in tumours with high tumour / stroma cyclooxygenase 2 IDV ratio , while a higher percentage of mast cells was detected in tumours showing high tumour / stroma cyclooxygenase 2 IDV ratio . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
For instance , CD 4 ( + ) CD 25 ( + ) regulatory T cells have received attention due to their immunosuppressive properties in vitro and in vivo , but in several instances it has been shown that CD 4 ( + ) CD 25 ( ) T cell populations also contain potent regulatory activity . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Studies of suppressor T cell function have been hampered until their recent identification as a minor fraction ( approximately 10 % ) of CD 4 ( + ) T cells that coexpress CD 25 . ^^^ CD 4 ( + ) CD 25 ( + ) T cells have been shown to play a critical role in the prevention of organ specific autoimmunity and allograft rejection . ^^^ Because tumor antigens are self antigens , it is not surprising that CD 4 ( + ) CD 25 ( + ) T cells also inhibit the induction of tumor immunity . ^^^ The spectrum of activity of CD 4 ( + ) CD 25 ( + ) cells extends to non self antigens , including infectious agents . ^^^ Although CD 4 ( + ) CD 25 ( + ) T cells are capable of inhibiting T ( H ) 2 responses , their role in the suppression of allergic responses has not been firmly established . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In this work we demonstrate that blockade of the CD 40 CD154 pathway at the time of transplantation enables the generation of donor alloantigen specific CD 4 ( + ) CD 25 ( + ) regulatory T cells , and that if the regulatory cells are present in sufficient numbers they can suppress allograft rejection mediated by CD 154 independent CD 8 ( + ) T cells . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Our data suggest that termination of BMP2 / 4 signaling is necessary for differentiation of CD 44 ( + ) CD 25 ( ) CD 4 ( ) CD 8 ( ) double negative ( DN ) cells along the T cell lineage . ^^^ Neutralization of endogenous BMP 2 and BMP 4 by treatment with the antagonist Noggin promotes and accelerates thymocyte differentiation , increasing the expression of CD 2 and the proportion of CD 44 ( ) CD 25 ( ) DN cells and CD 4 ( + ) CD 8 ( + ) double positive cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Likewise , in vivo depletion of CD 4 ( + ) CD 25 ( + ) T cells in BALB / c mice does not increase survival , demonstrating that CD 4 ( + ) CD 25 ( + ) cells do not regulate immunity . ^^^ Therefore , immunosurveillance in STAT 6 ( / ) mice facilitates survival against metastatic cancer via an IFN gamma dependent mechanism involving hemopoietic and nonhemopoietic derived cells , and is not exclusively dependent on counteracting IL 13 or CD 4 ( + ) CD 25 ( + ) T cells . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The three key features of this inhibition of activation are : First , late events in T cell activation ( after 18 h ) are affected ; second , these cells do not undergo anergy ; and third , CD 4 ( + ) CD 25 ( + ) regulatory T cells are not responsible . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Here we show that this ability , assessed by retinal ganglion cell survival after optic nerve injury or locomotor activity after spinal cord contusion , is decreased if the animals were immunized as neonates with myelin proteins ( resulting in their nonresponsiveness as adults to myelin proteins ) or injected with naturally occurring regulatory CD 4 ( + ) CD 25 ( + ) T cells immediately after the injury , and is improved by elimination of these regulatory T cells . ^^^ In nude BALBc mice replenished with a splenocyte population lacking CD 4 ( + ) CD 25 ( + ) regulatory T cells , significantly more neurons survived after optic nerve injury than in nude mice replenished with a complete splenocyte population or in matched wild type controls . ^^^ In contrast , neuronal survival in wild type BALBc mice injected with CD 4 ( + ) CD 25 ( + ) regulatory T cells immediately after injury was significantly worse than in noninjected controls . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In vitro chemotaxis experiments using immune lymph node ( LN ) cells showed that RANTES attracted mTg specific responder LN cells , whereas MCP 1 attracted mTg specific CD 4 ( + ) , CD 25 ( + ) regulator cells that secreted IL 10 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Several types of Tr cells exist , including CD 4 ( + ) T cells which express CD 25 constitutively and suppress immune responses via direct cell to cell interactions , and type 1 T regulatory ( Tr 1 ) cells , which function via secretion of interleukin ( IL ) 10 and transforming growth factor ( TGF ) beta . ^^^ The relationship between CD 25 ( + ) CD 4 ( + ) T cells and Tr 1 cells remains unclear . ^^^ Here , we demonstrate at the clonal level that Tr 1 and CD 25 ( + ) CD 4 ( + ) T cells are two distinct subsets of regulatory cells with different cytokine production profiles . ^^^ Furthermore , CD 25 ( ) CD 4 ( + ) T cells can be rendered anergic by IL 10 and differentiated into Tr 1 cells in the absence of CD 25 ( + ) CD 4 ( + ) T cells . ^^^ Cloned human CD 25 ( + ) CD 4 ( + ) T cell populations are heterogeneous and only a subset of clones continues to express high levels of CD 25 and is suppressive . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Interestingly , amongst circulating memory CD 4 ( + ) CD45RO ( + ) T cells , chemotactic responsiveness to CCL 1 is restricted to cells expressing CD 25 and / or CLA surface markers for regulatory T cells ( Treg ) and skin homing T cells and maximal responsiveness is observed on CLA ( + ) CD 25 ( + ) T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Inhibition of human CD 4 ( + ) CD 25 ( +high ) regulatory T cell function . ^^^ CD 4 ( + ) CD 25 ( +high ) T cells are potent regulators of autoreactive T cells . ^^^ However , it is unclear how regulatory CD 4 ( + ) CD 25 ( +high ) cells discriminate between desirable inflammatory immune responses to microbial Ags and potentially pathologic responses by autoreactive T cells . ^^^ In this study , an in vitro model was created that allowed differential activation of regulatory CD 4 ( + ) CD 25 ( +high ) and responder CD 4 ( + ) T cells . ^^^ If CD 4 ( + ) CD 25 ( +high ) regulatory cells were strongly activated , they maintained suppressive effector function for only 15 h , while stimulation with weaker TCR stimuli produced regulatory cells that were suppressive until 60 h after activation . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Here we show that CD 4 ( + ) CD 25 ( + ) T cells , negatively regulating antifungal Th 1 reactivity , are generated in mice with candidiasis . ^^^ CD 4 ( + ) CD 25 ( + ) T cells were not generated in B 7 2 or CD 28 deficient mice or in condition of IL 10 signaling deficiency . ^^^ CD 4 ( + ) CD 25 ( + ) T cells poorly proliferated in vitro ; were highly enriched for cells producing IL 4 , IL 10 , and TGF beta ; and required IL 10 producing , Candida hypha activated dendritic cells for generation . ^^^ Adoptive transfer of CD 4 ( + ) CD 25 ( + ) T cells or IL 10 producing dendritic cells restored resistance to reinfection and decreased inflammation in B 7 2 deficient mice . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Immunocytochemical analysis showed a significant increase of CD 2 , CD 4 CD 8 , CD 25 and HLA DR positive cells with rejection . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
AGC 10 also decreased the intracellular levels of CD 25 , CD 122 , and CD 132 in CD 4 ( + ) and CD 8 ( + ) cells stimulated with the T cell mitogen phytohemagglutinin ( PHA ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Differences in CD 25 and CD11a expression indicate that the CD 4 ( + ) cells from the lung airways and parenchyma are distinct memory populations . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Autoimmunity on alert : naturally occurring regulatory CD 4 ( + ) CD 25 ( + ) T cells as part of the evolutionary compromise between a ' need ' and a ' risk ' . ^^^ Likewise , the naturally occurring regulatory CD 4 ( + ) CD 25 ( + ) T cells have a physiological function , and are not merely an evolutionary adaptation to suppress self reactive T cell clones that escaped deletion in the thymus . ^^^ Downregulation or upregulation of CD 4 ( + ) CD 25 ( + ) Tr cells might be a way to achieve better protection from neurodegenerative conditions induced by self destruction or avoid autoimmune inflammatory disease development , respectively . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Stimulation with PPD or PWM increased CD 25 and CD 44 mean fluorescence intensity ( MFI ) and decreased CD62L MFI on CD 4 ( + ) cells from infected animals . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The re expressed CD 28 was functional and restored the ability of CD 4 ( + ) CD 28 ( null ) T cells to express CD 25 and CD 40 ligand . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) regulatory T ( T ( R ) ) cells can inhibit a variety of autoimmune and inflammatory diseases , but the precise mechanisms by which they suppress immune responses in vivo remain unresolved . ^^^ Here , we have used Helicobacter hepaticus infection of T cell reconstituted recombination activating gene ( RAG ) ( / ) mice as a model to study the ability of CD 4 ( + ) CD 25 ( + ) T ( R ) cells to inhibit bacterially triggered intestinal inflammation . ^^^ H . hepaticus infection elicited both T cell mediated and T cell independent intestinal inflammation , both of which were inhibited by adoptively transferred CD 4 ( + ) CD 25 ( + ) T ( R ) cells . ^^^ T cell independent pathology was accompanied by activation of the innate immune system that was also inhibited by CD 4 ( + ) CD 25 ( + ) T ( R ) cells . ^^^ Thus , CD 4 ( + ) CD 25 ( + ) T ( R ) cells do not only suppress adaptive T cell responses , but are also able to control pathology mediated by innate immune mechanisms . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Graft acceptance is associated with an increased percentage of CD 4 ( + ) CD 25 ( + ) regulatory cells in the spleen and in the draining lymph node that can protect 100 % of syngeneic recipients from islet allograft rejection . ^^^ CD 4 ( + ) CD 25 ( + ) cells , able to inhibit the T cell response to a pancreatic autoantigen and to significantly delay disease transfer by pathogenic CD 4 ( + ) CD 25 ( ) cells , are also induced by treatment of adult nonobese diabetic ( NOD ) mice with 1 , 25 dihydroxy 16 , 23Z diene 26 , 27 hexafluoro 19 nor vitamin D ( 3 ) ( BXL 698 ) . ^^^ The enhancement of CD 4 ( + ) CD 25 ( + ) regulatory T cells , able to mediate transplantation tolerance and to arrest type 1 diabetes development by a short oral treatment with VDR ligands , suggests possible clinical applications of this approach . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Mononuclear cells from the peripheral blood from all three groups were separated on Ficoll gradient and labelled for surface antigens ( i . e . cluster of differentiation ( CD ) 3 , CD 4 , CD 8 , CD 25 , CD 14 and human leucocyte antigen D receptor ( HLA DR ) ) using monoclonal antibodies . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Increases in splenic Treg and Tol DC were observed in tolerant recipients as assessed by an increase in CD 4 ( + ) CD 25 ( + ) T cells and DC with immature phenotype . ^^^ Tol DC purified from tolerant recipients incubated with naive T cells induced the generation / expansion of CD 4 ( + ) CD 25 ( + ) Treg . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Specifically , and contrary to published reports , the thymocyte subset distribution , the rate and composition of thymic export , and the composition of the peripheral T cell pool , including the proportion of CD 25 ( + ) CD 4 ( + ) T cells , are essentially normal in prediabetic NOD mice . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
OBJECTIVE : We aimed to investigate if there is an alteration in CD 4 and CD 8 T cell populations and in CD 25 and CD49d activation antigens linked to these cells during an acute attack of Mnire ' s disease ( MD ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Lymphocyte proliferation , expression of T cell surface activation antigens ( CD 25 , CD 71 , CD11a , CD 95 , CD 154 ) , production of intracellular cytokines ( IL 2 , INFgamma , TNFalpha ) , and lymphocyte subsets ( CD 4 , CD 8 , CD 16 , CD 20 ) were assessed by flow cytometry . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Reduced expression of CD 25 on CD 4 ( + ) T cells after infection of B7KO mice was consistent with deficits in T cell activation to effector functions . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Compared with controls , IL 2 induced a greater increase of naive and memory CD 4 T cells , lymphocyte expression of CD 28 and CD 25 ( P < 0 . 0001 ) and natural killer cells ( P < 0 . 001 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Recently , a brief period of CD40L mAb treatment was reported to induce tolerance and enhancement of CD 4 ( + ) CD 25 ( + ) regulatory T cell activity . ^^^ We therefore determined the efficacy of CD40L mAb treatment in autoimmunity that resulted from CD 4 ( + ) CD 25 ( + ) regulatory T cell deficiency . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We report in this study that approximately 70 % of CD 4 ( + ) CD 25 ( ) T cell preparations from individual 24 mo old mice are hyporesponsive to in vitro stimulation with anti CD 3 Ab . ^^^ The remaining 30 % of CD 4 ( + ) CD 25 ( ) T cell preparations showing the intermediate or normal responsiveness in the primary stimulation also exhibit the hyporesponsive properties after primary stimulation . ^^^ Both of these hyporesponsive aged CD 4 ( + ) CD 25 ( ) T cells could inhibit the proliferation of cocultured CD 4 ( + ) CD 25 ( ) T cells from young mice , like CD 4 ( + ) CD 25 ( + ) T cells , which have recently been demonstrated as an immune regulator in young mice . ^^^ Another experiment revealed that hyporesponsive aged CD 4 ( + ) CD 25 ( ) T cells arrest the cell division of cocultured young CD 4 ( + ) CD 25 ( ) T cells . ^^^ The suppressive activity observed in aged CD 4 ( + ) CD 25 ( ) T cells is aging dependent , not mediated by humoral factors , cell contact dependent , and broken by the addition of IL 2 or anti GITR Ab , but not by anti CTLA 4 Ab . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Furthermore , enzymatically active ( < 0 . 1 U / ml ) but not E 64 inactivated CPB2 . 8 was able to proteolytically cleave CD 23 and CD 25 , although not B 220 or CD 4 from murine lymphocytes . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Of interest , the greatest intensity of CTLA 4 expression occurred in CD 4 ( + ) CD 25 ( + ) cells , a population purported to include suppressor cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The immunoregulatory properties of CD 4 ( + ) CD 25 ( + ) and CD 4 ( + ) CD 25 ( ) T cells were characterized by proliferation and suppression assays . ^^^ RESULTS : Patients with epithelial malignancies show an increase of CD 4 ( + ) CD 25 ( + ) T cells in the peripheral blood with characteristics of Tregs , i . e . , they are CD45RA ( ) , CTLA 4 ( + ) , and transforming growth factor beta ( + ) . ^^^ Notably , CD 4 ( + ) T cells from cancer patients are characterized by an impaired proliferative capacity , which is restored to the extend of CD 25 depleted CD 4 ( + ) T cells from control persons by prior removal of CD 25 ( + ) T cells . ^^^ In contrast to CD 4 ( + ) CD 25 ( ) T cells , isolated CD 4 ( + ) CD 25 ( + ) T cells from cancer patients were anergic towards T cell receptor stimulation . ^^^ In addition , CD 4 ( + ) CD 25 ( + ) T cells suppressed the proliferation of CD 4 ( + ) CD 25 ( ) T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Routine analysis and lymphocyte phenotyping by an immunoperoxidase technique with the monoclonal antibodies CD 3 , CD 20 , CD 4 , CD 8 , CD 57 , CD 1 , CD 25 , CD 71 and HLA DR were performed . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Cytokines were measured in the cell culture supernatant by means of ELISA , and CD 4 ( + ) CD 25 ( + ) cells were analyzed by means of flow cytometry . ^^^ Maternal D pteronyssinus immunization induced a significant decrease of the IFN gamma level in the offspring , avoided an exacerbation of T ( H ) 2 cytokine secretion , and , concomitantly , upregulated the number of CD 4 ( + ) CD 25 ( + ) T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We report that the subset of CD 4 cells known to exert regulatory functions in vivo ( CD45RB ( low ) CD 25 ( + ) ) selectively express Toll like receptors ( TLR ) 4 , 5 , 7 , and 8 . ^^^ Exposure of CD 4 ( + ) CD 25 ( + ) cells to the TLR 4 ligand lipopolysaccharide ( LPS ) induces up regulation of several activation markers and enhances their survival / proliferation . ^^^ Most importantly , LPS treatment increases CD 4 ( + ) CD 25 ( + ) cell suppressor efficiency by 10 fold and reveals suppressive activity in the CD 4 ( + ) CD45RB ( low ) CD 25 ( ) subset that when tested ex vivo , scores negative . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Murine CD 4 ( + ) CD 25 ( + ) regulatory cells have been reported to express latency associated peptide ( LAP ) and TGF beta on the surface after activation , and exert regulatory function by the membrane bound TGF beta in vitro . ^^^ We have now found that a small population of CD 4 ( + ) T cells , both CD 25 ( + ) and CD 25 ( ) , can be stained with a goat anti LAP polyclonal Ab without being stimulated . ^^^ In the CD 4 ( + ) CD45RB ( high ) induced colitis model of SCID mice , regulatory activity was exhibited not only by CD 25 ( + ) LAP ( + ) and CD 25 ( + ) LAP ( ) cells , but also by CD 25 ( ) LAP ( + ) cells . ^^^ CD 4 ( + ) CD 25 ( ) LAP ( + ) T cells were part of the CD45RB ( low ) cell fraction . ^^^ CD 4 ( + ) CD 25 ( ) LAP ( ) CD45RB ( low ) cells had minimal , if any , regulatory activity in the colitis model . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Removal of one group of regulatory cells , the CD 25 ( + ) CD 4 ( + ) T cells , leads to autoimmune manifestations in experimental animal models , and reintroduction of this population prevents disease . ^^^ This study addresses the role of such regulatory T cells in humans with an autoimmune disease , where we demonstrate the presence of CD 25 ( bright ) CD 4 ( + ) T cells in the target organ of patients with active rheumatoid arthritis . ^^^ The patients displayed an enrichment of CD 25 ( bright ) CD 4 ( + ) T cells in synovial fluid as compared to peripheral blood . ^^^ Although the frequency of CD 25 ( bright ) CD 4 ( + ) T cells varied between patients , it was found to be constant over time in any one joint during each relapse . ^^^ In summary , it is striking that in addition to all activated , potentially pathological T cells the synovial fluid from RA patients also contains CD 25 expressing CD 4 ( + ) T cells with a regulatory capacity . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) T cells from both nave and tolerised donors are able to transfer tolerance to grafts in lymphopenic recipients , and this may be directly applicable to bone marrow transplantation . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Hepatitis B virus ( HBV ) specific CD 4 ( + ) T cells were ex vivo generated by activating with HBV transfected dendritic cells and selecting with antibodies to CD 25 activation molecules , and then expanded with antibodies to CD3 / CD28 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Functional alteration of peripheral CD 25 ( + ) CD 4 ( + ) immunoregulatory T cells in a transgenic rat model of autoimmune diseases . ^^^ Murine peripheral CD 25 ( + ) CD 4 ( + ) T cells play roles in maintaining immunological self tolerance . ^^^ To examine if alterations of immunoregulatory cells may be evident in env pX rats , quantitative and qualitative analyses of splenic CD 25 ( + ) CD 4 ( + ) T cells were done before these rats developed autoimmune diseases . ^^^ Env pX and non transgenic rats had equivalent number of CD 25 ( + ) CD 4 ( + ) T cells . ^^^ However , CD 25 ( + ) CD 4 ( + ) T cells from env pX rats did not suppress proliferation of T cells stimulated by anti CD 3 antibodies ( Ab ) in vitro , whereas those from non transgenic rats did . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The number of CD 3 ( + ) , CD 4 ( + ) , CD 8 ( + ) , CD 25 ( + ) , CD 71 ( + ) and CD 26 ( + ) lymphocytes was also evaluated . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We demonstrate that adult human CD25+ cells regulate the response to myelin oligodendrocyte glycoprotein ( MOG ) , as depletion of CD 25 ( + ) cells increases responses of PBMC and the addition of purified CD25+ cells suppresses MOG specific proliferation and IFN gamma production of CD 4 ( + ) CD 25 ( ) T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) T cells play a central role in the suppression of autoimmunity and inflammation , making their in vivo expansion a highly attractive therapeutic target . ^^^ By phenotyping with a novel rat CTL antigen 4 ( CTLA 4 ) specific monoclonal antibody ( mAb ) and functional in vitro assays , we here first establish that rat CD 4 ( + ) CD 25 ( + ) T cells correspond to the regulatory T cells ( Treg cells ) described in mice and humans : they constitutively express CTLA 4 , produce IL 10 but not IL 2 , and are able to suppress the proliferation of costimulated CD 25 negative indicator cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Our choice of topics in basic immunology included the description of T bet as a determinant factor for T ( H ) 1 differentiation , the role of the activation induced cytosine deaminase gene in B cell development , the characterization of CD 4 ( + ) CD 25 ( + ) regulatory T cells , and the use of dynamic imaging to study MHC class 2 transport and T cell and dendritic cell membrane interactions . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Monoclonal antibodies specific for human T lymphocytes and subpopulations : CD 2 , CD 3 , CD 4 , CD 8 , CD 19 and activation markers : CD 25 , CD45RA , CD45RO , CD 54 AND HLA ( ) DR were used and detected using a two colour fluorescence analysis with an automated flow cytometer . ^^^ PE is associated with elevated levels of CD 4 ( + ) HLA ( ) DR ( + ) , and CD 3 ( + ) NK cells but decreased total numbers of T lymphocytes , and the CD 3 ( + ) CD 25 ( + ) subpopulation . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) T cells co expressing CD 25 ( CD 4 ( + ) CD 25 ( + ) T cells ) have been identified as immunoregulatory suppressors modulating autoimmune response . ^^^ Based on these data , we hypothesised that CD 4 ( + ) CD 25 ( + ) T cells may influence protective immunity to malaria parasites , while suppressing autoimmune response arising throughout the course of malarial infection . ^^^ To test this possibility , we evaluated the kinetics of CD 4 ( + ) CD 25 ( + ) T cells during malaria infection and investigated the influence of CD 25 depletion by anti mouse CD 25 monoclonal antibody ( PC 61 ) on the infection , using a mouse model of premunition to Plasmodium berghei NK 65 malaria . ^^^ The results showed that , during exacerbation of P . berghei NK 65 infection , the proportion of CD 4 ( + ) CD 25 ( + ) T cells among CD 4 ( + ) T cells decreased , although that of CD 4 ( + ) T cells increased . ^^^ These findings demonstrated that CD 4 ( + ) CD 25 ( + ) T cells are able to influence protective immunity underlying premunition to P . berghei NK 65 parasites . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The subpopulation of CD 4 T cells , characterized by a constitutive expression of CD 25 , plays a critical role in the regulation of multiple auto immune diseases . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Both viruses induced similar humoral and cellular immune responses in the host , but only RV also caused a decrease in splenic CD 4 ( + ) CD 25 ( + ) T cells in both BBDR rats and normal WF rats . ^^^ Surprisingly , RV infection increased CD 4 ( + ) CD 25 ( + ) T cells in pancreatic lymph nodes of BBDR but not WF rats . ^^^ This increase appeared to be due to the accumulation of nonproliferating CD 4 ( + ) CD 25 ( + ) T cells . ^^^ The results imply that the reduction in splenic CD 4 ( + ) CD 25 ( + ) cells observed in RV infected animals is virus specific , whereas the increase in pancreatic lymph node CD 4 ( + ) CD 25 ( + ) cells is both virus and rat strain specific . ^^^ More generally , the results suggest a mechanism that could link an underlying genetic predisposition to environmental perturbation and transform a `` regulated predisposition ' ' into autoimmune diabetes , namely , failure to maintain regulatory CD 4 ( + ) CD 25 ( + ) T cell function . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
A unique population of CD 4 ( + ) T lymphocytes that constitutively express CD 25 has been recognized as anergic / suppressor cells . ^^^ While the immunosuppressive activity of these CD 4 ( + ) CD 25 ( + ) cells has been validated and implicated in tolerance , autoimmunity , transplantation , cancer and infectious diseases , the mechanism ( s ) by which they function still remains controversial . ^^^ Although the involvement of TGF beta was initially discounted , emerging evidence now links this cytokine with CD 4 ( + ) CD 25 ( + ) T cell mediated suppression of antigen activated T cells . ^^^ Collectively , multiple previously unconnected puzzle pieces are beginning to be linked into a more coherent , albeit incomplete picture of CD 4 ( + ) CD 25 ( + ) T cell mediated suppression . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
OPP clones bear a CD 4 ( + ) CD 25 ( + ) phenotype and show significantly lower proliferative response compared to T ( h ) 0 clones . ^^^ Thus , antigen specific CD 4 ( + ) CD 25 ( + ) T ( reg ) , which have characteristics of anergic cells and actively suppress antibody production are induced in PP upon oral administration of protein antigen . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The lymphocyte markers CD 3 , CD 4 , CD 8 , CD 16 , CD 19 , CLA , CD 25 , CD 69 , CD45RO , CD45RA were determined by flow cytometry . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) regulatory T cells have been shown to prevent T cell mediated immune pathology ; however , their ability to ameliorate established inflammation has not been tested . ^^^ Using the CD 4 ( + ) CD45RB ( high ) T cell transfer model of inflammatory bowel disease , we show that CD 4 ( + ) CD 25 ( + ) but not CD 4 ( + ) CD 25 ( ) CD45RB ( low ) T cells are able to cure intestinal inflammation . ^^^ Transfer of CD 4 ( + ) CD 25 ( + ) T cells into mice with colitis led to resolution of the lamina propria infiltrate in the intestine and reappearance of normal intestinal architecture . ^^^ CD 4 ( + ) CD 25 ( + ) T cells were found to proliferate in the mesenteric lymph nodes and inflamed colon . ^^^ These studies suggest that manipulation of CD 4 ( + ) CD 25 ( + ) T cells may be beneficial in the treatment of chronic inflammatory diseases . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The BiAb targeted to the thyroid most likely facilitated engagement of CTLA 4 , resulting in an increase in the number of CD 4 ( + ) CD 25 ( + ) T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The expression of C2gamma or C2zeta on the surface of CD 4 ( + ) T cells was down regulated upon binding CII , and cells activated in such a way proliferated , up regulated CD 25 expression and produced cytokines . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
BACKGROUND : Recently , it has been established that CD 4 ( + ) CD 25 ( + ) T cells with regulatory capacity are present in human peripheral blood , inhibiting allogeneic proliferation and cytokine production of preactivated CD 4 ( + ) CD 25 ( ) respond er T cells . ^^^ OBJECTIVE : The aim of this study was to analyze in an allergen specific setting whether such regulatory CD 4 ( + ) CD 25 ( + ) T cells also exist and function normally in atopic individuals , especially concerning the inhibition of T ( H ) 2 cytokines . ^^^ METHODS : For this purpose , CD 4 ( + ) CD 25 ( ) or CD 4 ( + ) CD 25 ( + ) T cells from donors allergic to grass or birch pollen ( mainly with rhinitis ) or from healthy nonatopic donors were stimulated in the presence of autologous , mature , monocyte derived , allergen pulsed dendritic cells , and the preactivated CD 4 ( + ) CD 25 ( + ) T cells were added to CD 4 ( + ) CD 25 ( ) T cells during restimulation . ^^^ RESULTS : CD 4 ( + ) CD 25 ( + ) T cells from the nonatopic donors and from the majority of the patients investigated proliferated poorly , produced fewer cytokines , and inhibited the proliferation and T ( H ) 1 ( IFN gamma ) and T ( H ) 2 ( IL 4 and IL 5 ) cytokine production of CD 4 ( + ) CD 25 ( ) T cells but not IL 10 production . ^^^ The suppression of CD 4 ( + ) CD 25 ( ) T cells by CD 4 ( + ) CD 25 ( + ) T cells was at least partially antigen unspecific and not reversible with anti IL 10 , anti transforming growth factor beta , or anti cytotoxic T lymphocyte associated antigen 4 mAb but was reversible with IL 2 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
However , the expansion of CD 3 ( ) CD 4 ( ) CD 8 ( ) triple negative T cell precursors at the CD 44 ( ) CD 25 ( + ) and CD 44 ( ) CD 25 ( ) stages , and CD 4 ( + ) CD 8 ( + ) double positive thymocytes was affected during embryogenesis in these mutants . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The disease susceptibility neonatal time window was not related to a propensity of neonatal ovaries to autoimmune inflammation , and it was not affected by infusion of functional adult CD 4 ( + ) CD 25 ( + ) T cells . ^^^ However , resistance to neonatal AOD in 9 day old mice was abrogated by CD 4 ( + ) CD 25 ( + ) T cell depletion . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
PATIENTS AND METHODS : Lymphocyte subpopulations ( CD 3 , CD 4 , CD 8 , CD 16 , CD 19 , and CD 25 ) and plasma levels of soluble ( s ) interleukin ( IL ) 1 receptor antagonist ( RA ) , IL 2 , sIL 2R , IL 3 , IL 4 , IL 6 , sIL 6R , IL 8 , IL 10 , transforming growth factor beta ( 2 ) , tumor necrosis factor alpha , interferon gamma , and neopterin were studied in 52 CDT and 33 LDT recipients 1 to 2 , 4 to 6 , and 8 to 10 days after transplantation . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
To clarify whether this is due to the absence or functional impairment of antigen specific CD 4 ( + ) T cells we developed an assay that relies on the induced expression of the T cell activation marker CD 25 and is therefore independent from cytokine secretion or proliferation . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We sought to determine whether donor CD 4 ( + ) CD 25 ( + ) regulatory T cells could control ongoing GVHD , thereby providing an initial window of time in which the alloreactive anti host response is permitted to begin , with the intent of most effectively eliminating residual leukemia cells . ^^^ Prevention of lethal GVHD by infusion of donor CD 4 ( + ) CD 25 ( + ) cells early after HCT ( day 2 ) was achieved across a major histocompatibility complex barrier in the haploidentical C3H > ( B6xC3H ) F ( 1 ) model . ^^^ However , in vitro expansion of donor CD 4 ( + ) CD 25 ( + ) T cells , stimulated by recipient cells in the presence of high dose interleukin 2 , was required for successful regulation . ^^^ BR > CBA GVHD model , lethal disease could be completely prevented by a single infusion of freshly isolated donor CD 4 ( + ) CD 25 ( + ) cells administered as late as 10 days after HCT . ^^^ Of importance , this late regulatory effect required only a 3 : 1 ratio of effector CD 8 : CD 4 ( + ) CD 25 ( + ) T cells , indicating a strong potential for the delayed infusion of CD 4 ( + ) CD 25 ( + ) cells to control GVHD across minor histocompatibility antigen barriers . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In this variant of experiment , a great similarity has been established of changes in expression of mononuclear membrane markers ( CD 3 , CD 4 , CD 8 , CD 20 , CD 16 , HLA DR and to a lesser degree of CD 25 ) ; the ability has been proven of the photomodified blood to `` translate ' ' the light induced changes to a much higher volume of non irradiated blood , which might represent a mechanism of the systemic immunomodulating effect of phototherapy . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Graft acceptance is associated with impaired development of type 1 CD 4 ( + ) and CD 8 ( + ) cells and an increased percentage of CD 4 ( + ) CD 25 ( + ) regulatory cells expressing CD 152 in the spleen and in the draining lymph node . ^^^ Transfer of CD 4 ( + ) CD 25 ( + ) cells from tolerant mice protects 100 % of the syngeneic recipients from islet allograft rejection . ^^^ CD 4 ( + ) CD 25 ( + ) cells that are able to inhibit the T cell response to a pancreatic autoantigen and to significantly delay disease transfer by pathogenic CD 4 ( + ) CD 25 ( ) cells are also induced by treatment of adult nonobese diabetic ( NOD ) mice with a selected vitamin D receptor ( VDR ) ligand . ^^^ The enhancement of CD 4 ( + ) CD 25 ( + ) regulatory T cells able to mediate transplantation tolerance and to arrest type 1 diabetes development by a short oral treatment with small organic compounds that induce tolerogenic DCs , like VDR ligands , suggests possible clinical applications of this approach . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The degree of B cell dysfunction in viremic patients correlated strongly with the inability of B cells to express CD 25 in response to activated CD 4 ( + ) T cells , resulting in an inability to mount a normal proliferative response to IL 2 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
However , although capable of efficiently restricting the fungal growth , mu MT mice did not survive the re infection with C . albicans , and this was concurrent with the failure to generate IL 10 producing dendritic cells and regulatory CD 4 ( + ) CD 25 ( + ) T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In addition , SIT induced an antigen specific suppressive activity in CD 4 ( + ) CD 25 ( + ) T cells of allergic individuals . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Thymus derived CD 4 ( + ) CD 25 ( + ) regulatory T ( T ( r ) ) cells play a critical role in suppressing aberrant responses to self in vivo . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
This feature is reminiscent of natural CD 4 ( + ) CD 25 ( + ) regulatory T cells . ^^^ However , it is not known whether the regulatory function of in vivo tolerized T cells is similar to the function of natural CD 4 ( + ) CD 25 ( + ) regulatory T cells . ^^^ In this study , we demonstrate that CD 4 ( + ) CD 25 ( + ) as well as CD 4 ( + ) CD 25 ( ) T cells isolated from mice treated with superantigen three consecutive times to induce tolerance were functionally comparable to natural CD 4 ( + ) CD 25 ( + ) regulatory T cells , albeit more potent . ^^^ Importantly , we demonstrate that conventional CD 4 ( + ) CD 25 ( ) T cells could also be induced to acquire regulatory functions by the same regimen in the absence of natural regulatory T cells in vivo , but that such regulatory cells were functionally different . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Transfer of splenic CD 4 ( + ) CD 25 ( + ) and CD 4 ( + ) CD 25 ( ) T cell subsets from OVA tolerized mice revealed that both subsets were equally able to suppress a delayed type hypersensitivity response in acceptor mice . ^^^ Together , these findings demonstrate a role for CD 4 ( + ) CD 25 ( ) T ( r ) cells in mucosal tolerance , which suppresses CD 4 ( + ) T cells in an antigen specific fashion , irrespective of initial T ( h ) 1 / T ( h ) 2 skewing of the immune response . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In the lung of double transgenic mice , a large number of Ag specific CD 4 T cells expressed CD 25 and functioned as regulatory T cells . ^^^ The CD 25 ( + ) CD 4 T cells suppressed proliferation of CD 25 ( ) CD 4 T cells in vitro and inhibited type 2 immune responses induced by aerosolized Ags in vivo . ^^^ Despite their ability to suppress allergic type 2 immunity in the airways , however , CD 25 ( + ) CD 4 regulatory T cells had no effect on the development of bronchial hyperreactivity . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
There was no difference in the percentage of CD 3 , CD 4 , CD 8 , CD 25 , CD45RO , CD 103 , and cutaneous lymphocyte Ag positive cells in PBMCs among the patients with asthma , dermatitis , rheumatoid arthritis , and normal subjects , and CD 3 positive cells in peripheral blood of asthmatic , dermatitis , rheumatic , and normal huPBMC SCID mice . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Further , stimulation of cells from Chagas ' disease patients with PFR enhanced the frequencies of both small and large CD 4 ( + ) CD 25 ( + ) and CD 4 ( + ) CD 69 ( + ) lymphocytes , as well as that of small CD 8 ( + ) CD 25 ( + ) lymphocytes . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) regulatory T cells have been shown to regulate a variety of autoimmune and allogeneic responses in mice and humans . ^^^ The role of CD 4 ( + ) CD 25 ( + ) cells in regulating alloresponses in human transplant recipients remains uncertain . ^^^ A number of mechanisms have been proposed to account for this finding ; the purpose of this study was to determine whether CD 4 ( + ) CD 25 ( + ) cells play a significant role . ^^^ Twelve stable renal transplant patients were investigated using limiting dilution assay ( LDA ) and ELISPOT for interferon gamma to determine the effect of depleting CD 4 ( + ) CD 25 ( + ) cells on the direct pathway alloresponse . ^^^ The percentage of CD 4 ( + ) CD 25 ( + ) cells in the peripheral blood of the study patients was equivalent to that of healthy controls . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Interestingly , the CD 25 ( + ) subset that corresponds to the CD 4 ( + ) regulatory T cells controlling autoimmune processes was lacking . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In humans and rodents a population of naturally occurring CD 4 ( + ) CD 25 ( + ) T cells are suppressor T ( CD 25 ( + ) Ts ) cells , which are considered to maintain peripheral immunological tolerance . ^^^ Expression of CCR 4 associates with both CD 25 and CLA cell surface markers , being highest on CD 4 ( + ) CLA ( + ) CD 25 ( + ) T cells . ^^^ Importantly , CD 4 ( + ) CD 25 ( + ) Ts cells isolated from human cord blood lack expression of CLA while expressing CCR 4 , suggesting intrinsic expression of CCR 4 on CD 25 ( + ) Ts cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Parameters of cellular and humoral immunity ( differential blood count , T cell markers CD 2 , CD 3 , CD 4 , and CD 8 , B cell markers CD 19 and CD 20 , monocyte markers CD 13 and CD 14 , NK cell marker CD 16 , activation markers CD 25 , CD 26 , CD 69 and HLA DR , and cytokines IL 1 receptor antagonist ( IL 1RA ) , IL 2 , soluble IL 2 receptor ( sIL 2R ) , IL 6 , IL 10 , and TGFbeta ) were measured in peripheral venous blood . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Day 3 thymectomy ( D3Tx ) leads to a paucity of CD 4 ( + ) CD 25 ( + ) suppressor T cells , a loss of peripheral tolerance , and the development of organ specific autoimmune disease in adult mice . ^^^ Given the roles of cathepsins in Ag processing and presentation , Stfa 1 and Stfa 2 alleles have the potential to control susceptibility to autoimmune disease at the level of both CD 4 ( + ) CD 25 ( + ) suppressor and CD 4 ( + ) CD 25 ( ) effector T cells . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
As for CD 4 ( + ) regulatory cells this defect was probably qualitative , because the percentages of CD 4 ( + ) CD 25 ( + ) or CD 4 ( + ) CD45RC ( low ) populations were normal after xenothymus transplantation . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In this study , we investigated whether CD 4 ( + ) CD 25 ( ) and CD 4 ( + ) CD 25 ( + ) T cells of human CD 4 ( + ) subpopulation could differentially express HO 1 . ^^^ Our results obtained from qualitative reverse transcriptase polymerase chain reaction and quantitative flow cytometry analyses revealed that the CD 4 ( + ) CD 25 ( + ) T cells constitutively express HO 1 and that T cell stimulation with plate bound anti CD 3 in combination with soluble anti CD 28 not only induced HO 1 gene expression in the CD 4 ( + ) CD 25 ( ) T cells but also up regulated HO 1 gene expression in the CD 4 ( + ) CD 25 ( + ) T cells . ^^^ Our further studies showed that CD 28 signal alone was enough to induce HO 1 expression and CD 3 signal , of which signal alone did not induce HO 1 expression , was required at least for full HO 1 expression in both CD 25 ( ) and CD 25 ( + ) subsets of human CD 4 ( + ) T cells . ^^^ Taken together , we have first reported that human CD 4 ( + ) CD 25 ( + ) regulatory T cells constitutively express HO 1 and that HO 1 inhibits Jurkat T cell proliferation . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Methods We performed an immunohistochemical study with a large panel of monoclonal antibodies to CD 3 , CD 4 , CD 8 , HLA DR , CD 25 , myeloperoxidase , tryptase , eosinophil cationic protein EG 2 , human interleukin ( IL ) 2 , 4 , 5 , 8 , interferon ( IFN ) gamma , and granulocyte macrophage colony stimulating factor using the alkaline phosphatase antialkaline phosphatase procedure on lesional skin of seven patients with PG . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) regulatory T cells and their function in maintaining transplantation tolerance ] . ^^^ This article reviews that as a functionally and phenotypically distict immunoregulatory T cell subpopulation , CD 4 ( + ) CD 25 ( + ) regulatory T cells can suppress the activation and proliferation of CD 4 ( + ) CD 25 ( ) T cells and CD 8 ( + ) T cells and the production of IL 2 and IFN gamma . ^^^ CD 4 ( + ) CD 25 ( + ) regulatory T cells can be isolated , activated and expanded in vitro without loss of their immunoregulatory function . ^^^ The suppressive function of activated CD 4 ( + ) CD 25 ( + ) cells is antigen non specific . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) regulatory T cells in normal animals are engaged in the maintenance of immunological self tolerance and prevention of autoimmune disease . ^^^ However , accumulating evidence suggests that a fraction of the peripheral CD 4 ( + ) CD 25 ( ) T cell population also possesses regulatory activity in vivo . ^^^ Recently , it has been shown glucocorticoid induced TNFR family related gene ( GITR ) is predominantly expressed on CD 4 ( + ) CD 25 ( + ) regulatory T cells . ^^^ In this study , we show evidence that CD 4 ( + ) GITR ( + ) T cells , regardless of the CD 25 expression , regulate the mucosal immune responses and intestinal inflammation . ^^^ Interestingly , both CD 4 ( + ) CD 25 ( + ) and CD 4 ( + ) CD 25 ( ) GITR ( + ) T cells prevented wasting disease and colitis . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
This potentially pathogenic population of Ag experienced T cells is subject to T cell mediated regulation in vivo by both CD 4 ( + ) CD 25 ( + ) and CD 4 ( + ) CD 25 ( ) cells in an IL 10 dependent manner . ^^^ Interestingly , IL 10 was not absolutely required for CD 4 ( + ) CD 25 ( + ) T cell mediated inhibition of colitis induced by transfer of naive CD 4 ( + ) CD45RB ( high ) cells , suggesting a differential requirement for IL 10 in the regulation of naive and Ag experienced T cells . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Ferritin levels were evaluated by ELISA and regulatory T cell numbers were assessed by three color flow cytometry to identify CD 4 ( + ) CD 25 ( + ) CD 69 ( ) T cells . ^^^ CD 69 positive cells were excluded to avoid inclusion of normal activated CD 4 , CD 25 expressing T cells . ^^^ In addition , these studies revealed a marked increase in the number of CD 4 ( + ) CD 25 ( + ) CD 69 ( ) T cells in such patients , compared with age matched controls . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Although there were no differences in CD 4 ( + ) or CD 8 ( + ) T cell counts or the percentage of cells that were CD 4 ( + ) CD 25 ( + ) , CD 8 ( + ) CD 25 ( + ) , or CD 4 ( + ) DR ( + ) , patients who received SIT had a significantly higher percentage of CD 8 ( + ) CD 38 ( + ) and CD 8 ( + ) DR ( + ) cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Hearts transplanted into Tg recipients showed decreased CD 4 ( + ) lymphocyte infiltration and diminished immune activation , as judged by CD 25 expression . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Medians ( range ) of positive cells were for CD 3 : 20 . 5 ( 0 530 . 0 ) , CD 4 : 6 . 2 ( 0 124 . 4 ) , CD 8 : 1 . 8 ( 0 81 . 5 ) , CD 25 : 0 ( 0 62 . 3 ) , HLA DR : 80 . 0 ( 3 . 5 524 . 2 ) , EG 1 : 5 . 3 ( 0 180 . 6 ) , EG 2 : 6 . 4 ( 0 48 . 8 ) , AA 1 : 51 . 3 ( 0 286 . 4 ) , CD 45 : 39 . 7 ( 0 448 . 5 ) and CD45ro : 28 . 6 ( 0 425 . 2 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Prednisone decreased levels of proinflammatory cytokines during IL 2 cycles but , despite induction of expression of CD 25 , blunted increases in IL 2 associated CD 4 ( + ) T lymphocyte count . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Cellularity and dendritic cell content in DLNC population , as well as the expression of TCR alpha , CD 4 , CD 8 and CD 25 , were not changed between irradiated and nonirradiated animals . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 25 ( + ) CD 4 ( + ) regulatory T cells have major roles in controlling immune responses , and use heterogeneous regulatory mechanisms . ^^^ Here we show that CD 103 ( + ) CD 25 ( + ) CD 4 ( + ) T cells ( that control inflammatory bowel disease ) are highly enriched in gut associated lymphoid tissue and have unique functional properties . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In our previous report , we showed that CD 4 ( + ) CD 25 ( ) T cells in aged ( 24 month old ) mice , especially after in vitro pre stimulation of these cells , exhibit hyporesponsive and suppressive properties . ^^^ We examined here whether the suppressive activity of aged CD 4 ( + ) CD 25 ( ) T cells is ascribable to a particular population within these cells . ^^^ In vitro analyses revealed that cell populations rapidly extruding Rhodamine 123 ( R 123 ) ( referred to as R 123 ( lo ) cells ) in aged CD 4 ( + ) CD 25 ( ) T cells have a more potent suppressive function compared with R 123 ( hi ) populations . ^^^ In addition , CD 103 ( + ) cells in freshly prepared aged CD 4 ( + ) CD 25 ( ) R 123 ( lo ) T cells had a most potent suppressive activity . ^^^ Furthermore , the R 123 ( lo ) population in young CD 4 ( + ) CD 25 ( ) T cells also had different properties from R 123 ( hi ) T cells : low responsiveness , no additive effect in proliferation assays , and the gain of a suppressive function after in vitro pre stimulation . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The role of CD 4 ( + ) CD 25 ( + ) regulatory T cells in Leishmania infection . ^^^ There is growing evidence that regulatory T cells and , in particular , the endogenous CD 4 ( + ) CD 25 ( + ) T cells ( T ( reg ) ) are playing a fundamental role in the infectious process due to the protozoan parasite Leishmania . ^^^ Endogenous CD 4 ( + ) CD 25 ( + ) T cells are a population of regulatory T cells , recently described for their capacity to control excessive or misdirected immune response . ^^^ Such control occurs by modulation of the effector immune response ; in susceptible mouse strains , CD 4 ( + ) CD 25 ( + ) T cells suppress excessive T helper ( Th ) 2 response , while in genetically resistant mouse strains , they control protective Th 1 responses , allowing for parasite survival and maintenance of memory response . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
There are two broad categories of naturally occurring and induced CD 4 ( + ) CD 25 ( + ) regulatory T cells . ^^^ Induced T ( R ) are either CD 4 ( + ) CD 25 ( + ) or CD 8 ( + ) , produce immunosuppressive cytokines such as IL 10 , act directly on other T cells or APC and are antigen specific in some but not in all systems . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We show here that such tolerogenic DC anergize alloreactive CD 4 ( + ) CD45RO ( + ) CD 25 ( + ) T cells converting them into regulatory T cells ( T ( R ) ) , which in turn , continue the cascade of suppression by tolerizing other DC . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
These CD 4 ( + ) CD 25 ( + ) regulatory T cells inhibit a variety of autoimmune and inflammatory diseases and they are also efficient in the suppression of alloantigen responses . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The latter cells express CD 4 , CD 25 and CTLA 4 . ^^^ These CD 4 ( + ) CD 25 ( + ) T cells are similar in origin , phenotype , and function to those that maintain natural self tolerance and T cell homeostasis in the periphery . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We studied a group of 42 patients with SSc and a group of 28 matched normal controls by flow cytometry using the lymphocyte cell surface markers CD 2 , CD 3 , CD 4 , CD 8 , CD 19 , CD 25 , CD45RA , CD 56 , CD 71 , HLA DR , TCR alpha / beta , and TCR gamma / delta . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD28 / B7 blockade leads to exacerbated autoimmune disease in the nonobese diabetic mouse strain as a result of a marked reduction in the number of CD 4 ( + ) CD 25 ( + ) regulatory T cells ( Tregs ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
A population of CD 25 ( + ) CD 4 ( + ) regulatory T cells ( T regs ) functions to maintain immunological self tolerance by inhibiting autoreactive T cell responses . ^^^ CD 25 ( + ) CD 4 ( + ) T regs are present in low , but steady , numbers in the peripheral lymphoid tissues of healthy mice . ^^^ How this cytokine functions to regulate CD 25 ( + ) CD 4 ( + ) T reg homeostasis and prevent autoimmune disease remains unknown . ^^^ We show here that bcl 2 deficiency does not affect CD 25 ( + ) CD 4 ( + ) T reg homeostasis , and that ectopic expression of this molecule fails to rescue CD 25 ( + ) CD 4 ( + ) T reg numbers or to prevent the development of autoimmunity in IL 2 deficient mice . ^^^ Furthermore , transient activation of STAT 5 is sufficient to increase CD 25 ( + ) CD 4 ( + ) T reg numbers in IL 2 deficient mice . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
However , the suppressor cells differ from the natural CD 4 ( + ) T regulatory cells , described recently in human and mouse , because they do not express cell surface CD 25 . ^^^ By contrast , after repeated SEB injection , CD 4 ( + ) CD 25 ( + ) splenocytes were heterogenous and contained both effector as well as regulatory cells . ^^^ In vivo , CD 4 ( + ) CD 25 ( ) T regulatory cells prevented SEB induced death independently of CD 4 ( + ) CD 25 ( + ) T cells . ^^^ Collectively , these data demonstrate that , whereas CD 4 ( + ) CD 25 ( + ) T regulatory cells are required for the induction of SAg induced tolerance , CD 4 ( + ) CD 25 ( ) T cells exert their regulatory activity at the maintenance stage of SAg specific unresponsiveness . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Within the inhibitory population , CD 4 ( + ) CD 25 ( + ) and CD 8 ( + ) CD 25 ( ) T cells had the greatest activity . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Glucocorticoid induced tumor necrosis receptor ( GITR ) has been implicated in regulation of T cell suppression by CD 25 ( + ) CD 4 ( + ) regulatory T cells ( Tregs ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) regulatory lymphocytes require interleukin 10 to interrupt colon carcinogenesis in mice . ^^^ Recent studies have shown that microbially driven inflammation can lead to colon cancer and that prior transfer of regulatory lymphocytes expressing CD 4 and CD 25 prevents the innate inflammatory events that lead to colon cancer in mice . ^^^ Mice were then treated at 1 , 3 , or 12 months after infection with adoptive transfer of CD 4 ( + ) CD45RB ( lo ) CD 25 ( + ) regulatory cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In the nonobese diabetic ( NOD ) mouse , pathogenic and suppressor CD 4 ( + ) T cells can be distinguished by the constitutive expression of CD 25 . ^^^ CD 4 ( + ) CD 25 ( + ) suppressor T cells from 8 , but not 16 wk old NOD mice delayed the onset of diabetes transferred by 16 wk old CD 25 depleted spleen cells . ^^^ These results were paralleled by the inhibition of alloantigen induced proliferation of CD 4 ( + ) CD 25 ( ) cells , indicating an age dependent decrease in suppressive activity . ^^^ In addition , CD 4 ( + ) CD 25 ( ) pathogenic T cells became progressively less sensitive to immunoregulation by CD 4 ( + ) CD 25 ( + ) T cells during diabetes development . ^^^ CD 4 ( + ) CD 25 ( ) T cells showed a higher proliferation and produced more IFN gamma , but less IL 4 and IL 10 , whereas CD 4 ( + ) CD 25 ( + ) T suppressor cells produced significantly lower levels of IL 10 in 16 compared with 8 wk old NOD mice . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Increased IgG2a Ab titer in the sera of tumor free animals after vaccination and elevated CD 4 ( + ) CD 25 ( + ) regulatory T cells in the PBL of tumor bearing animals suggested that IFN gamma producing Th 1 cells may be responsible for partial protection of CD 4 ( + ) T cells against the mammary tumor challenge , whereas CD 4 ( + ) CD 25 ( + ) regulatory T cells ( Th 2 cells ) may suppress the antitumor immune responses . ^^^ Together , these results suggest that HSP 110 ICD complex can elicit effective IFN gamma producing T cells against spontaneous mammary tumors and that up regulation of CD 4 ( + ) CD 25 ( + ) regulatory T cells may prevent complete eradication of the tumor following immunotherapy . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In ADA deficient FTOCs initiated at day 15 of gestation , thymocyte development was arrested at the CD 4 ( ) CD 8 ( ) CD 44 ( lo ) CD 25 ( + ) to CD 4 ( ) CD 8 ( ) CD 44 ( lo ) CD 25 ( ) transition . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Regulatory T cells ( T regs ) , characterized by coexpression of CD 4 and CD 25 markers , can inhibit the immune response mediated by CD4+ / CD25 and CD8+ T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We review the evidence for the emergence of regulatory cells , such as CD 4 ( + ) CD 25 ( + ) T cells during infection or during induction of tolerance by mucosal antigen administration . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Thymic derived dysregulated tolerance has been suggested to occur in type 1 diabetes via impaired generation of CD 4 ( + ) CD 25 ( + ) T regulatory cells , leading to autoimmune beta cell destruction . ^^^ In this study , we demonstrate that Notch 3 expression is a characteristic feature of CD 4 ( + ) CD 25 ( + ) cells . ^^^ The failure to develop the disease is associated with an increase of CD 4 ( + ) CD 25 ( + ) T regulatory cells , accumulating in lymphoid organs , in pancreas infiltrates and paralleled by increased expression of IL 4 and IL 10 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) DO11 . 10 T cells from CLN were phenotypically similar to CD 4 ( + ) DO11 . 10 T cells from ILN , however , in CLN a higher percentage of CD 25 ( + ) proliferating CD 4 ( + ) DO11 . 10 T cells were detected compared with ILN . ^^^ CD 25 is not a discriminative marker for mucosal T ( R ) cells because both CD 25 ( + ) and CD 25 ( ) CD 4 ( + ) DO11 . 10 T cells from the CLN could suppress delayed type hypersensitivity responses in adoptive transfer . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In addition to the naturally occurring CD 4 ( + ) CD 25 ( + ) population , several subtypes of induced regulatory cells have been reported , but their mechanisms of action remain unclear . ^^^ These data demonstrate that cytotoxic CD 4 ( + ) CD 25 ( + ) T cells that lack proliferation capacities have the potential to down regulate an immune response by killing Ag presenting B cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
These effects were mediated , at least in part , by dampening of the antigen specific immunosuppressive function of CD 4 ( + ) CD 25 ( + ) regulatory T cell populations . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The present study is aimed at exploring the regulatory CD 4 ( + ) CD 25 ( + ) T cells in the thymus from myasthenia gravis ( MG ) patients . ^^^ Preliminary studies indicate that these CD 4 ( + ) CD 25 ( + ) cells include activated autoreactive T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Several mechanisms have been tested as potential inhibitors , including CD 4 ( + ) CD 25 ( + ) T regulatory cells , IL 13 , a T ( h ) 2 shift , and myeloid suppressor cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( high ) T regulatory ( Tr ) cells , representing high IL 2 receptor alpha chain expressing cells , have been shown to inhibit proliferation and cytokine secretion by CD 4 ( + ) T cells that are assumed to represent important effector cells in auto aggressive immunity . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In addition to decreases in CSF albumin and IgG levels , treatment resulted in an increase of CD 8 ( + ) CXCR 3 ( + ) cells as well as a decrease in CD 4 ( + ) subsets expressing CD 25 , CD 29 , and CCR 4 in the CSF . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Using a syngeneic ( Lewis to Lewis ) rat lung transplant model , we observed that recipient CD 4 ( + ) T cells infiltrated lung grafts within 1 h of reperfusion and up regulated the expression of CD 25 over the ensuing 12 h . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In this study , we provide direct evidence that CD 4 ( + ) CD 25 ( + ) regulatory T cells can cure an established , severe , and progressive colitis . ^^^ SCID mice developed severe colitis when adoptively transferred with naive CD 4 ( + ) CD 25 ( ) T cells and infected with the protozoan parasite Leishmania major . ^^^ The disease development can be completely halted and symptoms reversed , with a healthy outcome , by transferring freshly isolated or activated CD 4 ( + ) CD 25 ( + ) T cells from syngeneic donors . ^^^ These results therefore demonstrate that CD 4 ( + ) CD 25 ( + ) T cells are therapeutic and that the effect is mediated by both IL 10 / TGF beta dependent and independent mechanisms . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
There is now compelling evidence that CD 4 ( + ) CD 25 ( + ) T cells play a major role in the maintenance of tolerance . ^^^ Besides CD 4 ( + ) CD 25 ( + ) T cells , different populations of regulatory CD 4 ( + ) T cells secreting high amounts of IL 10 ( T regulatory type 1 ( Tr 1 ) ) or TGF beta ( Th 3 ) have also been described in in vivo models . ^^^ In contrast , inflammation was not controlled when high numbers of CD 4 ( + ) CD45RB ( low ) or CD 4 ( + ) CD 25 ( + ) T cells were injected as early as 1 wk after the start of the experiment . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Because IL 2 signaling is thought to contribute to tolerance through maintenance of a CD 4 ( + ) CD 25 ( + ) regulatory T cell population , we examined these cells and observed a numerical reduction in STAT5A / 5B ( / ) mice along with a higher rate of apoptosis . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Depletion of CD 4 ( + ) CD 25 ( + ) Treg cells with CD 25 mAb resulted in mammary tumor regression and increased susceptibility to thyroiditis . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In vivo administration of anti CD 25 mAb ( PC 61 ) caused regression of RL male 1 , suggesting that CD 4 ( + ) CD 25 ( + ) immunoregulatory cells were involved in the tumor growth . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In PHA activated lymphocytes , CD 4 expression was increased , whereas CD 8 and IL 2R beta chain ( CD 25 ) expression were decreased . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Peripheral blood lymphocytes were double stained for CD 4 or CD 8 , as well as CD11a , CD 25 , CD 26 , CD 29 and CD45RA or the chemokine receptors CCR 3 , CCR 4 , CCR 5 or CXCR 3 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
After cell count and differentiation of second morning urine samples with New Sternheimer and Papanicolaou stains , immunocytochemistry was performed using antibodies against the following antigens : CD 2 , CD 4 , CD 8 , CD 25 , CD 71 ( transferrin receptor ) , HLA DR and cytokeratin ( Lu 5 ) . ^^^ Immunocytochemical analysis showed a significant increase in CD 2 , CD 4 , CD 8 , CD 25 , CD 71 , and HLA DR positive epithelial cells and in the ratio HLA DR / cytokeratin positive epithelial cells in rejection . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
A spectrum of CD 4 ( + ) T cells , including Th 3 cells , T ( R ) cells , CD 4 ( + ) CD 25 ( + ) cells and NKT cells play a critical role in regulating these diseases . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) regulatory T cell selection is initiated by high specificity interactions with self peptides in the thymus , although how these cells respond to cytokine derived signals and to re exposure to self peptide : MHC complexes in the periphery is not well understood . ^^^ We have used a transgenic mouse system , in which the peptide that induces thymic selection of a clonal population of CD 4 ( + ) CD 25 ( + ) regulatory T cells is known , to show that CD 4 ( + ) CD 25 ( + ) T cells proliferate in response to their selecting self peptide in vivo . ^^^ That their selecting self peptide is both required for and promotes the peripheral expansion of CD 4 ( + ) CD 25 ( + ) regulatory T cells may direct their accumulation in sites where the self peptide is expressed . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) T cells isolated from the peripheral blood of six healthy , nonallergic individuals showed a limited capacity to proliferate in response to nickel in vitro , but responsiveness was strongly augmented ( mean increment + / SD , 240 + / 60 % ) when cells were depleted of CD 25 ( + ) Treg . ^^^ The site of a negative patch test in response to nickel showed an infiltrate of CD 4 ( + ) CLA ( + ) cells and CD 25 ( + ) cells , which accounted for approximately 20 % of the total T cells isolated from the tissue . ^^^ Finally , CD 25 ( + ) T cells isolated from peripheral blood of nickel allergic patients showed a limited or absent capacity to suppress metal specific CD 4 ( + ) and CD 8 ( + ) T cell responses . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In addition , STAT 5 activation has dramatically divergent effects on CD 8 ( + ) vs CD 4 ( + ) T cells , leading to the selective expansion of CD 8 ( + ) memory like T cells and CD 4 ( + ) CD 25 ( + ) regulatory T cells . ^^^ These results establish that activation of STAT 5 is the primary mechanism underlying both IL 7 / IL 15 dependent homeostatic proliferation of naive and memory CD 8 ( + ) T cells and IL 2 dependent development of CD 4 ( + ) CD 25 ( + ) regulatory T cells . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 25 ( + ) regulatory T ( T reg ) cells suppress the activation / proliferation of other CD 4 ( + ) or CD 8 ( + ) T cells in vitro . ^^^ In this study , we show that depletion of CD 25 ( + ) T reg cells allows the host to induce both CD 4 ( + ) and CD 8 ( + ) antitumoral responses following tumor challenge . ^^^ Simultaneous depletion of CD 25 ( + ) and CD 8 ( + ) cells , as well as adoptive transfer experiments , revealed that tumor specific CD 4 ( + ) T cells , which emerged in the absence of CD 25 ( + ) T reg cells , were able to reject CT 26 colon cancer cells , a MHC class 2 negative tumor . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
O sialoglycoprotein endopeptidase , which removes the external domain of CD 43 and other O sialoglycoproteins from the aged naive CD 4 ( + ) T cells of TCR transgenic mice , restores early agonist independent stages and later agonist dependent stages of synapse formation as well as expression of the activation markers CD 69 and CD 25 to the levels found in the young mice . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Amongst the many types of regulatory cells that have been described during the past few years , the spontaneously occurring population that is characterized by co expression of CD 4 and CD 25 appears to play a key role in the prevention of autoimmunity and the maintenance of transplantation tolerance . ^^^ In this study we have examined the ability of CD 4 ( + ) CD 25 ( + ) T cells to regulate human CD 8 ( + ) T cells , and the behavior of CD 8 ( + ) T cells following activation in the presence of regulatory CD 4 ( + ) CD 25 ( + ) T cells . ^^^ The experiments described here demonstrate that human CD 4 ( + ) CD 25 ( + ) T cells cause pronounced and sustained inhibition of CD 8 ( + ) T cell proliferation in response to polyclonal and allogeneic stimulation . ^^^ These data provide insights into the short and long term effects of CD 4 ( + ) CD 25 ( + ) T cells on CD 8 ( + ) T cells that could be of considerable value in optimizing vaccination against tumor and viral antigens . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In vitro stimulation demonstrated that pure CD 44 ( + ) CD 25 ( ) , CD 44 ( + ) CD 25 ( + ) and CD 44 ( + ) CD 25 ( + ) thymocytes respond to ActivinA , while the mature CD 4 ( + ) and CD 8 ( + ) SP thymocytes respond to TGF beta stimulation measured as enhanced phosphorylation of Smad 2 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Moreover , such mice had increased numbers of CD 4 ( + ) sdLN cells that were CD 25 ( + ) , CD 28 ( + ) or CD 152 ( + ) and accessory cells that were CD 40 ( + ) or MHC 2 ( + ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Notably , HCV persistence was associated with a reversible CD 4 mediated suppression of HCV specific CD 8 T cells and with higher frequency of CD 4 ( + ) CD 25 ( + ) regulatory T cells ( 7 . 3 % chronic vs . 2 . 5 % recovered , P = . 002 ) that could directly suppress HCV specific type 1 CD 8 T cells ex vivo . ^^^ The high frequency of CD 4 ( + ) CD 25 ( + ) regulatory T cells and their suppression of HCV specific CD 8 T cells ex vivo suggests a novel role for regulatory T cells in HCV persistence . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The number of positive cells for CD 4 , CD 8 , CD 25 , CD 152 , and Fas ligand were determined using immunohistochemical stainings and imaging analysis techniques . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Finally , human naturally occurring regulatory CD 4 ( + ) CD 25 ( + ) T cells appeared important in generating regulatory T cells by costimulation blockade . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Immunocytochemistry revealed that brain tumors in mice previously vaccinated with AdB 7 infectants had been infiltrated by a larger number of CD 3 ( + ) lymphocytes and that these CD 3 ( + ) lymphocytes contained not only CD 4 ( + ) and CD 8 ( + ) T cells but also CD 25 ( + ) activated T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We investigated if MC is associated with a deficit of CD 4 ( + ) CD 25 ( + ) immunoregulatory T ( Treg ) cells , which have been shown to control autoimmunity . ^^^ Because Treg cells express higher amounts of CD 25 than activated CD 4 ( + ) T cells , we analyzed blood CD 4 ( + ) CD 25 ( high ) Treg cells in 69 untreated patients chronically infected with HCV . ^^^ In symptomatic MC patients , the purified remaining CD 4 ( + ) CD 25 ( + ) T cells retained suppressive activity in vitro . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Although a great deal has been learned as to how 4 1BB mediated signaling governs the immunity of conventional T cells , the functional role of 4 1BB in the context of CD 4 ( + ) CD 25 ( + ) regulatory T cell ( Tr ) activation is largely unknown . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Depleting mice of CD 25 ( + ) T cells with and without CTL associated Ag 4 ( CTLA 4 ) blockade demonstrated that tolerance of Tyr specific CTL was not regulated by CD 4 ( + ) CD 25 ( + ) T regulatory cells ( Treg ) or CTLA 4 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Here we show that depletion of CD 4 ( + ) CD 25 ( + ) regulatory T cells ( T ( reg ) ) protects mice from death when infected with a lethal strain of Plasmodium yoelii , and that this protection is associated with an increased T cell responsiveness against parasite derived antigens . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The peak of AVHA was characterized by relative lymphocytosis , by decreases in the counts of T helper / inductor cells and natural killer cells , by increases in the count of CD 25 cells , convalescence , by preserved lymphocytosis , by the increased levels of T lymphocytes that carry the markers CD 3 , CD 4 , CD 8 , CD 16 , CD 25 , and CD 95 , and by higher toxicity of beta 2 microglobulin . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
LY 411 , 575 decreased overall thymic cellularity and impaired intrathymic differentiation at the CD 4 ( ) CD 8 ( ) CD 44 ( + ) CD 25 ( + ) precursor stage . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) regulatory T ( Treg ) cells suppress naive T cell responses , prevent autoimmunity , and delay allograft rejection . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Ex vivo selection of recipient type alloantigen specific CD 4 ( + ) CD 25 ( + ) immunoregulatory T cells for the control of graft versus host disease after allogeneic hematopoietic stem cell transplantation . ^^^ CD 4 ( + ) CD 25 ( + ) immunoregulatory T cells , which play a pivotal role in preventing organ specific diseases , can also modulate GVHD if administered in equal numbers of T cells at the time of grafting . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Several autoimmune disease models depend on an imbalance in the activation of aggressor T ( h ) 1 and CD 4 ( + ) CD 25 ( + ) regulatory T ( T ( reg ) ) cells . ^^^ We used a colitis model in which disease causing CD45RB ( hi ) T cells alone or in combination with CD 4 ( + ) CD 25 ( + ) T cells from either CD 28 deficient or wild type donors were transferred into T cell deficient animals , half of which were treated with ICOS blocking reagents . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 25 ( + ) CD 4 ( + ) regulatory T cells inhibit the activation of autoreactive T cells in vitro and in vivo , and suppress organ specific autoimmune diseases . ^^^ The mechanism of CD 25 ( + ) CD 4 ( + ) T cells in the regulation of experimental autoimmune encephalomyelitis ( EAE ) is poorly understood . ^^^ To assess the role of CD 25 ( + ) CD 4 ( + ) T cells in EAE , SJL mice were immunized with myelin proteolipid protein ( PLP ) ( 139 151 ) to develop EAE and were treated with anti CD 25 mAb . ^^^ Anti CD 25 antibody treatment caused a decrease in the percentage of CD 25 ( + ) CD 4 ( + ) T cells in blood , peripheral lymph node ( LN ) and spleen associated with increased production of IFN gamma and a decrease in IL 10 production by LN cells stimulated with PLP ( 130 151 ) in vitro . ^^^ In addition , transfer of CD 25 ( + ) CD 4 ( + ) regulatory T cells from naive SJL mice decreased the severity of active EAE . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Our findings also suggest that insufficient expression of SOCS 1 results in impaired function of CD 25 ( + ) CD 4 ( + ) regulatory T cells , which may contribute to aberrant activation of CD 4 ( + ) T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 25 has become widely used as a marker for a subset of regulatory CD 4 ( + ) T cells present in the thymus and periphery of mice , rats and humans . ^^^ This study provides a detailed account of the phenotype of CD 4 ( + ) CD 25 ( + ) regulatory T cells in rats . ^^^ In the thymus , CD 4 ( + ) CD 8 ( ) CD 25 ( + ) cells were found to have a more mature phenotype than the corresponding CD 4 ( + ) CD 8 ( ) CD 25 ( ) cells with respect to expression of Thy 1 ( CD 90 ) , CD 53 and CD 44 , suggesting that CD 25 expression , and perhaps commitment to regulatory function , might be a late event in thymocyte development . ^^^ CD 4 ( + ) CD 25 ( + ) cells in both the thymus and periphery were found to have enriched and heterogeneous expression of activation markers such as OX 40 ( CD 134 ) and OX 48 ( an antibody determined in this study to be specific for CD 86 ) . ^^^ CD 4 ( + ) CD 25 ( + ) T cells were also found to have enriched expression of CD 80 , at both the mRNA and protein level . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Phenotypic and functional analyses demonstrated that they were antigen specific CD 4 ( + ) Treg cells expressing CD 25 and GITR molecules and possessing suppressive activity on the proliferative response of naive CD 4 ( + ) T cells to anti CD 3 antibody stimulation . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
OBJECTIVE : In the present study we compared the lymphocyte composition of tumor draining lymph nodes ( TDLN ) in 13 cervical ( CC ) and 26 endometrial cancer ( EC ) patients with special emphasis on the suppressive CD 4 ( + ) T cell subset constitutively expressing CD 25 ( T regulatory , Treg , cells ) . ^^^ RESULTS : Compared to EC patients , TDLN from CC patients contained a higher proportion of naive CD 4 ( + ) and CD 8 ( + ) cells ( P < 0 . 01 ) , a lower proportion of CD 4 ( + ) pre effector cells ( P < 0 . 05 ) , and a higher proportion of the most potent Treg cell subset identified by coexpression of a high level of CD 25 and CD 152 ( P < 0 . 05 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Shh is necessary for expansion of CD 4 ( ) CD 8 ( ) double negative ( DN ) thymocytes and for efficient transition from the earliest CD 44 ( + ) CD 25 ( ) DN population to the subsequent CD 44 ( + ) CD 25 ( + ) DN population and from DN to CD 4 ( + ) CD 8 ( + ) double positive cells . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We show here that PROb tumor volume is correlated with an expansion of CD 4 ( + ) CD 25 ( + ) regulatory T lymphocytes in lymphoid tissues . ^^^ A single administration of cyclophosphamide depletes CD 4 ( + ) CD 25 ( + ) T cells in PROb tumor bearing animals , delays the growth of PROb tumors , and cures rats bearing established PROb tumors when followed by an immunotherapy which has no curative effect when administered alone . ^^^ These results demonstrate the role of CD 4 ( + ) CD 25 ( + ) regulatory T cells in tumor induced immune tolerance and the interest of regulatory T cell depletion to sensitize established tumors to immunotherapy . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The in vivo differentiation / survival of CD 4 ( + ) CD 25 ( + ) T suppressor cells is dependent on IL 2 and CD 28 mediated costimulatory signals . ^^^ In the subsequent culture , activated CD 4 ( + ) CD 25 ( + ) cells were then mixed with responders in order to assess their suppressor function . ^^^ Inhibition of the interaction of CD 28 or cytotoxic T lymphocyte associated antigen 4 ( CTLA 4 ) with CD80 / CD86 in the pre culture of CD 4 ( + ) CD 25 ( + ) cells did not prevent the induction of suppressor function . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Emerging data have unveiled a pivotal role for TGF beta as a perpetrator of suppression by CD 4 ( + ) CD 25 ( + ) regulatory T ( Treg ) cells and in apoptotic sequelae . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The secondary endpoints were renal function ; histological parameters related to CyA ; serum level of TGF beta , PDGF BB , blockade of CD 25 molecule and surface expression of CD 3 , CD 4 , CD 8 , CD 69 , CD11a , CD49d , CD 28 , CD 152 molecules in the subpopulations of T cells in the peripheral blood . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Thymus derived regulatory T lymphocytes of CD 4 ( + ) CD 25 ( + ) phenotype regulate a large variety of beneficial and deleterious immune responses and can inhibit lethal graft versus host disease in rodents . ^^^ In vitro , CD 4 ( + ) CD 25 ( + ) T cells require specific major histocompatibility complex ( MHC ) / peptide ligands for their activation , but once activated they act in an antigen nonspecific manner . ^^^ Here we show that CD 4 ( + ) CD 25 ( + ) regulatory T lymphocytes isolated from naive mice and activated in vitro with allogeneic antigen presenting cells ( APCs ) induced specific long term tolerance to bone marrow grafts disparate for major and minor histocompatibility antigens ; whereas `` target ' ' bone marrow was protected , third party bone marrow was rejected . ^^^ These results indicate that CD 4 ( + ) CD 25 ( + ) regulatory T cells can act in an antigen specific manner in vivo . ^^^ Our results suggest that CD 4 ( + ) CD 25 ( + ) regulatory T cells could in the future be used in clinical settings to induce specific immunosuppression . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
During T cell responses to alloantigens expressed on dendritic cells , we observed that Abs against CD 86 potently enhanced suppression by CD 4 ( + ) CD 25 ( + ) Treg . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
This is characterized by an increase in thymocytes with the early development stage phenotype of CD 25 ( ) CD 44 ( + ) CD 4 ( ) CD 8 ( ) in aged IL 12b ( / ) mice . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Importantly , most of the CD 4 ( + ) T cell produced IL 10 was confined to a subset of T cells expressing CD 25 . ^^^ Nevertheless , when naive , naturally occurring CD 4 ( + ) CD 25 ( + ) cells were depleted in adoptive transfers , recipient IL 10 / RAG2 deficient animals were more susceptible than RAG 2 deficient mice , confirming an additional host protective role for non T cell derived IL 10 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The generation of CD 4 ( pos ) T cells with regulatory function by autologous stimulation did not require the presence of natural CD 4 ( pos ) CD 25 ( pos ) regulatory T cells . ^^^ In addition , the acquisition of regulatory function by CD 4 ( pos ) CD 25 ( neg ) T cells stimulated by autologous mature DC was accompanied by the induction of FOXP 3 expression . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( bright ) regulatory T cells are known to play an important role in the development and maintenance of tolerance in peripheral tissues . ^^^ However , the potential role of CD 4 ( + ) CD 25 ( bright ) T cells in maintaining human pregnancy has not been reported . ^^^ In this study , we show that early human pregnancy decidua contains an abundance of CD 4 ( + ) CD 25 ( bright ) T cells , which express CD 152 ( CTLA 4 ) at a high level . ^^^ CD 4 ( + ) CD 25 ( bright ) T cells mediate potent inhibition of autologous T cell proliferation by anti CD 3 stimulation . ^^^ Furthermore , these cells inhibit the proliferation of autologous CD 4 ( + ) CD 25 ( ) T cells in a dose dependent fashion . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Further , BV LPS induced CD 25 and Foxp 3 expression in lymphocytes and CD 4 ( + ) CD 25 ( + ) cells expressed IL 10 mRNA . ^^^ Thus , the low expression of functioning LPS receptor molecules and induction of IL 10 producing CD 4 ( + ) CD 25 ( + ) lymphocytes by indigenous LPS may play a central role in the maintenance of colonic immunological homeostasis . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) T cells that constitutively express CD 25 exhibit powerful suppressive properties . ^^^ A particular characteristic is that CD 4 ( + ) CD 25 ( + ) T cells inhibit the proliferative responses of CD 4 ( + ) CD 25 ( ) T cells by suppressing the capacity of the responders to transcribe IL 2 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Biopsies were taken before treatment and after four weeks of treatment , and markers for epidermal proliferation ( Ki 67 ) and epidermal differentiation ( keratin 10 ) were studied using a quantitative image analysis , and T cell subsets in epidermis and dermis ( CD 4 , CD 8 , CD 25 , CD45RO , CD45RA , CD 94 , CD 161 , and CD 2 ) were studied using immunohistochemical scoring . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
This suggestion received further support from the findings that irradiation was accompanied by an increased incidence of activated T cells in the lymphoid organs and peripheral blood and an increase in mRNA encoding for the pro inflammatory cytokines interleukin 12 and interferon gamma , and that after irradiation , passive transfer of a subpopulation of suppressive T cells ( naturally occurring regulatory CD 4 ( + ) CD 25 ( + ) T cells ) wiped out the irradiation induced protection . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Recent studies of these cells , especially CD 4 ( + ) CD 25 ( + ) T cells and NKT cells , have revealed molecular and cellular mechanisms of immunosuppression . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The essential role played by CD 25 ( + ) CD 4 ( + ) regulatory T cells ( T ( R ) cells ) in the control of physiological as well as pathological immunity is now well established , but many aspects of their biology still remain unclear . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Experimental animal models for autoimmunity have demonstrated the existence and crucial role of CD 4 ( + ) CD 25 ( + ) T regulatory ( Tr ) cells in suppressing autoreactive T cells and promoting peripheral tolerance . ^^^ Recent in vitro functional studies showed that Tr cells are enriched in the CD 25 ( high ) cell population among CD 4 ( + ) T cells , and that they totally inhibit proliferation and cytokine secretion by CD 4 ( + ) T cells . ^^^ This study was done firstly to determine whether alterations of the CD 4 ( + ) CD 25 ( high ) T cells occur in MS , examining their frequencies . ^^^ As it was reported that the suppressive activity of CD 4 ( + ) CD 25 ( + ) Tr cells is mainly through cell surface contact pathway , we secondly analyzed the expression of the functionally important cell surface molecules of CD 4 ( + ) CD 25 ( high ) Tr cells . ^^^ Two or three colour flow cytometry was used to identify and quantify CD 4 ( + ) CD 25 ( + ) Tr cells and CD 4 ( + ) CD 25 ( high ) Tr cells among blood CD 4 ( + ) T cells in MS patients without treatment vs . patients treated with either interferon beta ( IFN beta ) or glatiramer acetate ( GA ) or IFN beta + GA in combination vs . healthy controls ( HC ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Regulatory T lymphocytes expressing CD 4 and CD 25 antigens and naturally present in the peripheral blood were the first to be phenotypically characterized . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In vitro expanded human CD 4 ( + ) CD 25 ( + ) T regulatory cells can markedly inhibit allogeneic dendritic cell stimulated MLR cultures . ^^^ CD 4 ( + ) CD 25 ( + ) T regulatory ( Treg ) cells have been shown to critically regulate self and allograft tolerance in several model systems . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
This apparent paradox has recently been resolved with the discovery that IL 2 is critical for the development and peripheral expansion of CD 4 ( + ) CD 25 ( + ) regulatory T cells , which promote self tolerance by suppressing T cell responses in vivo . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) regulatory T cells ( T ( reg ) ) can inhibit a variety of autoimmune and inflammatory diseases , but their involvement in regulating virus induced immunopathology is not known . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Using this method , we found the earliest thymocyte subset ( CD 4 ( ) CD 8 ( ) CD 25 ( ) CD 44 ( + ) ) to contain both IL 7 responsive and nonresponsive cells . ^^^ Transition through the next stages of development ( CD 4 ( ) CD 8 ( ) CD 25 ( + ) CD 44 ( + and ) ) was associated with responsiveness of all thymocytes within these populations . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Indeed , CD 4 ( + ) CD 25 ( + ) Treg , which are at the very center of this fascination , have emerged as a dominant T cell population capable of mediating peripheral tolerance to autoantigens , but whose functions have now been extended to regulation of T cell responses directed to foreign antigens . ^^^ However , a number of fundamental questions concerning the origin , phenotypic nature and mechanism of action of CD 4 ( + ) CD 25 ( + ) Treg cells remain elusive and misunderstood . ^^^ We propose the existence of two general subsets of CD 4 ( + ) CD 25 ( + ) Treg cells , naturally occurring and induced , that differ in their origin , developmental and activation requirements , and mechanism of action . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In addition to the well established role of natural CD 4 ( + ) CD 25 ( + ) regulatory T ( Tr ) cells in the maintenance of tolerance to self antigens , there is accumulating evidence for distinct populations of Tr cells induced in the periphery after encounter with pathogens and foreign antigens . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The suppression of immune response can be adoptively transferred with CD 4 ( + ) cells and does not partition with the CD 25 phenotype . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Although the phenotypic and regulatory properties of the CD 4 ( + ) CD 25 ( + ) T cell lineage ( Treg cells ) have been well described , the specificities remain largely unknown . ^^^ We demonstrate here that the CD 4 ( + ) CD 25 ( + ) Treg population includes the recognition of a broad spectrum of human TCR CDR 2 determinants found in the germline 5 gene repertoire as well as that of a clonotypic nongermline encoded CDR3beta sequence present in a recombinant soluble T cell receptor ( TCR ) protein . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Studies in experimental animal models of human autoimmune diseases have revealed that CD 4 ( + ) CD 25 ( + ) T regulatory ( Tr ) cells are of thymic origin and have potentials in preventing auto aggressive immunity . ^^^ We determined the frequencies and properties of circulating CD 4 ( + ) CD 25 ( + ) versus CD 4 ( + ) CD 25 ( ) cells in MG patients and healthy controls ( HCs ) , with special focus on the effect of thymectomy on CD 4 ( + ) CD 25 ( + ) cells . ^^^ CD 4 ( + ) CD 25 ( high ) cells comprise only about 2 % of blood lymphocytes in both MG patients and HCs . ^^^ Frequencies of CD 4 ( + ) CD 25 ( high ) cells were similar in MG patients irrespective of treatment with thymectomy . ^^^ CD 4 ( + ) CD 25 ( + ) cells in both MG patients and HCs are mainly memory T cells and are activated to a greater extent than CD 4 ( + ) CD 25 ( ) cells , as reflected by high levels of CD45RO and human leucocyte antigen ( HLA ) DR positive cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We show that age dependent reduction in effective precursor numbers depends on the one hand , extensive , activation dependent apoptosis occurring from the very onset of stimulation and , on the other , the accumulation of non dividing , regulatory ( suppressor ) CD 4 ( lo ) CD 25 ( + ) CD 28 ( lo ) T cells . ^^^ In addition , analysis of changes in surface expression of activation related antigens , including CD 25 , CD 69 , and PCNA in consecutive generations of dividing CD 4 ( + ) cells traced by carboxyfluorescein diacetate succinimidyl ester staining showed variable patterns of these changes that may relate to various aspect of impaired division of these cells in elderly . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Preferential replication of FIV in activated CD 4 ( + ) CD 25 ( + ) T cells independent of cellular proliferation . ^^^ In the present study , we investigated the susceptibility of naive and activated CD 4 ( + ) cell subsets ( distinguished by differential expression of CD 25 ) to feline immunodeficiency virus ( FIV ) infection , their ability to replicate the virus , and potentially act as a reservoir for virus persistence in infected animals . ^^^ While both CD 4 ( + ) CD 25 ( + ) and CD 4 ( + ) CD 25 ( ) cells are susceptible to FIV infection in vitro and in vivo , only CD 4 ( + ) CD 25 ( + ) cells produce infectious virions when cultured with interleukin 2 ( IL 2 ) . ^^^ Latently infected CD 4 ( + ) CD 25 ( ) cells produce infectious virions following ConcanvalinA ( ConA ) stimulation , which correlates with upregulated surface expression of CD 25 . ^^^ In contrast to CD 4 ( + ) CD 25 ( ) cells , CD 4 ( + ) CD 25 ( + ) cells remain unresponsive to mitogen stimulation and are relatively resistant to apoptosis whether or not infected with FIV . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The immunophenotype of peripheral blood mononuclear cells ( CD 4 , CD 38 , CD 8 , CD 23 , CD 25 , CD 57 , CD 30 , HLA DR ) was analyzed by flow cytometry . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
However , in circumstances of established tolerance , viral vaccines could break CD 8 tolerance in the presence of CD 4 ( + ) CD 25 ( + ) regulatory T cells , whereas dendritic cell based vaccines achieved this only after removal of regulatory T cells or the coadministration of a Toll like receptor ( TLR ) ligand or irrelevant virus . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The characteristics and functions of CD 4 ( + ) CD 25 ( + ) regulatory cells have been well defined in murine and human systems . ^^^ However , the interaction between CD 4 ( + ) CD 25 ( + ) T cells and dendritic cells ( DC ) remains unclear . ^^^ In this study , we examined the effect of human CD 4 ( + ) CD 25 ( + ) T cells on maturation and function of monocyte derived DC . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The glucocorticoid induced TNFR ( GITR ) is expressed at high levels on resting CD 4 ( + ) CD 25 ( + ) T regulatory ( T ( R ) ) cells and regulates their suppressive phenotype . ^^^ Importantly , naive CD 4 ( + ) CD 25 ( ) T cells up regulated GITR expression in an activation dependent manner and anti GITR mAb treatment enhanced the level of CD 4 ( + ) T cell activation , proliferation , and cytokine production in the absence of T ( R ) cells both in vivo and in vitro . ^^^ Taken together , these findings suggest a dual functional role for GITR as GITR cross linking both inactivates T ( R ) cells and increases CD 4 ( + ) CD 25 ( ) T cell effector function , thus enhancing T cell immunity . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The CD 4 ( + ) CTLA 4 ( + ) B 7 ( + ) phenotype described in FIV ( + ) cats is reminiscent of CD 4 ( + ) CD 25 ( + ) CTLA 4 ( + ) cells , a phenotype described for immunosuppressive T regulatory ( Treg ) cells . ^^^ In the present study , we describe the phenotypic and functional characteristics of CD 4 ( + ) CD 25 ( + ) T cells in PBMC and lymph nodes ( LN ) of FIV ( + ) and control cats . ^^^ Similar to Treg cells , feline CD 4 ( + ) CD 25 ( + ) but not CD 4 ( + ) CD 25 ( ) T cells directly isolated from LN of FIV ( + ) cats do not produce IL 2 and fail to proliferate in response to mitogen stimulation . ^^^ Unstimulated CD 4 ( + ) CD 25 ( + ) T cells from FIV ( + ) cats significantly suppress the proliferative response and the IL 2 production of Con A stimulated autologous CD 4 ( + ) CD 25 ( ) T cells compared with unstimulated CD 4 ( + ) CD 25 ( + ) T cells from FIV ( ) cats . ^^^ Flow cytometric analysis confirmed the apparent activation phenotype of the CD 4 ( + ) CD 25 ( + ) cells in LN of chronically FIV ( + ) cats , because these cells showed significant up regulation of expression of costimulatory molecules B7 . 1 , B7 . 2 , and CTLA 4 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The effect is based on ablation of a replicating dominantly suppressive CD 4 ( + ) T cell population , as indicated by adoptive transfer and in vivo depletion experiments using mAbs against CD 4 as well as combinations of mAbs against the known regulatory cell surface markers CD 25 , GITR , and CTLA 4 . ^^^ Cell surface marker analysis shows a population of CD 4 ( + ) CD 25 ( + ) cells arising shortly before day 14 p . i . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
RESULTS : HR 8348 cell line did not express CD 2 , CD 3 , CD 4 , CD 5 , CD 7 , CD 8 , TCR , CD 10 , CD11b , CD 14 , CD 16 , CD 19 , CD 22 , CD 25 , CD 28 , SmIg , CD 33 , CD 35 , CD 36 , CD41a , CD 45 , CD45RA , CD45RO , CD 56 , CD 61 , CD 64 , CD66b , CD 69 , CD 71 , CD 117 , CD 122 and P glycoprotein but expressed CD 13 , CD 15 , CD 20 , CD 38 , CD 95 and HLA DR . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Furthermore , CD 4 ( + ) CD 25 ( + ) T regulatory cells are not removed by this method of selective allodepletion and retain their function in suppressing allogeneic proliferative responses . ^^^ The retention of immunosuppressive CD 4 ( + ) CD 25 ( + ) T regulatory cells could lead to more ordered immune reconstitution and further suppress alloreactive responses after transplantation . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
HCT 1026 treatment was associated to ( 1 ) decreased mRNA levels of pro inflammatory cytokines , caspase 1 , and iNOS in blood cells ; ( 2 ) decreased ability of encephalitogenic T cells to proliferate ; ( 3 ) reduced number of central nervous system ( CNS ) infiltrating T cells ; ( 4 ) decreased axonal loss and demyelination ; ( 5 ) increased CD 4 ( + ) CD 69 ( ) CD 25 ( + ) regulatory T cells in the spleen . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Both CD 4 and CD 8 TILs were predominantly CD 44 ( High ) and expressed CD 25 , CD 69 , and CD 95 cell surface activation markers . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) regulatory cells are a subpopulation of T lymphocytes of thymic origin . ^^^ In the present work , we demonstrate that TGF beta is able to induce Foxp 3 expression and subsequently a regulatory phenotype in CD 4 ( + ) CD 25 ( ) peripheral murine T cells . ^^^ Similarly , TGF beta induced Foxp 3 in human CD 4 ( + ) CD 25 ( ) T cells . ^^^ Foxp 3 mediated down regulation of Smad 7 subsequently rendered CD 4 ( + ) CD 25 ( ) T cells highly susceptible to the morphogenic and regulatory effects of TGF beta signaling via Smad3 / 4 . ^^^ In summary , we demonstrate that TGF beta induces a regulatory phenotype in CD 4 ( + ) CD 25 ( ) T cells through the induction of Foxp 3 and a positive autoregulatory loop of TGF beta signaling due to the absence of Smad7 . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Thymus derived , natural CD 4 ( + ) CD 25 ( + ) regulatory T cells can educate peripheral CD 4 ( + ) CD 25 ( ) cells to develop suppressive activity by poorly understood mechanisms . ^^^ In this study , we report that CD 4 ( + ) CD 25 ( + ) cells are the targets of the costimulatory effects of IL 2 and TGF beta . ^^^ These cells do not divide , but , instead , greatly increase the numbers of CD 4 ( + ) CD 25 ( ) cells that become CD 25 ( + ) cytokine independent suppressor cells . ^^^ These CD 4 ( + ) CD 25 ( + ) regulatory cells , in turn , induce other alloactivated CD 4 ( + ) CD 25 ( ) cells to become potent suppressor cells by mechanisms that , surprisingly , require both cell contact and TGF beta and IL 10 . ^^^ The suppressive effects of these secondary CD 4 ( + ) CD 25 ( + ) cells depend upon TGF beta and IL 10 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
However , it is not known if CD 4 ( + ) CD 25 ( + ) regulatory T cells ( T ( reg ) ) recognize the same Ag as effector T cells or whether Ag specific TCR repertoire modification occurs in T ( reg ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Despite expression of the high affinity IL 2R , CD 4 ( + ) CD 25 ( + ) regulatory T cells ( Tregs ) are hypoproliferative upon IL 2R stimulation in vitro . ^^^ However the mechanisms by which CD 4 ( + ) CD 25 ( + ) T cells respond to IL 2 signals are undefined . ^^^ In this report , we examine the cellular and molecular responses of CD 4 ( + ) CD 25 ( + ) Tregs to IL 2 . ^^^ IL 2R stimulation results in a G ( 1 ) cell cycle arrest , cellular enlargement and increased cellular survival of CD 4 ( + ) CD 25 ( + ) T cells . ^^^ Negative regulation of phosphatidylinositol 3 kinase signaling and IL 2 mediated proliferation of CD 4 ( + ) CD 25 ( + ) T cells is inversely associated with expression of the phosphatase and tensin homologue deleted on chromosome 10 , PTEN . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 , CD 8 , CD 25 , and cutaneous lymphocyte associated antigen expression on PBMCs was assessed by means of flow cytometry . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 25 expression distinguishes functionally distinct alloreactive CD 4 CD134 ( OX 40 ) T cell subsets in acute graft versus host disease . ^^^ The data presented here reveal that differential expression of CD 25 by CD 4 ( + ) CD 134 ( + ) T cells allows separation of these activated cells into 2 phenotypically and functionally distinct alloreactive T cell subsets . ^^^ These subsets exhibit distinct tissue associations , with CD 4 ( + ) CD 134 ( + ) CD 25 ( ) T cells preferentially found in lymphoid tissues and CD 4 ( + ) CD 134 ( + ) CD 25 ( + ) T cells located in lymphoid tissues and inflamed extralymphoid tissues . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The T cell markers were characterized using a monoclonal antibody panel including CD 3 , CD 4 , CD 8 , CD 25 , CD 29 , CD 38 , CD45RA , CD45RO , CD 54 and HLA DR . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Cells taken from these latter mixed leukocyte cultures ( MLCs ) now contained CD 4 ( + ) CD 25 ( + ) cells able to inhibit the antigen specific MLC response of fresh C3H responder cells to stimulation with C57BL / 6 cells , but not stimulation with BALB / c cells . ^^^ The induction of CD 4 ( + ) CD 25 ( + ) suppressor cells in MLCs using tolerogenic DCs from the initial BM cultures could be overcome by using limiting numbers of tolerogenic DCs and an excess of allostimulatory DCs derived from BM cultures maintained in the absence of anti CD200R . ^^^ These data indicate that anti CD200R biases stem cells in BM toward the development of suppressive antigen presenting cells , which can induce CD 4 ( + ) CD 25 ( + ) regulatory T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Infectious tolerance is a contact dependent mechanism used by naturally occurring CD 25 ( + ) T regulatory cells ( Tregs ) to confer suppressive activity upon conventional CD 4 ( + ) T cells thereby generating secondary T helper suppressor cells ( Th ( sup ) ) , which inhibit T cell activation via soluble mediators . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Suggestive of a causal role for CD 40 in autoimmunity , blocking CD 40 CD154 interactions early during NOD development prevents autoaggressive T cell expansion while promoting increases in CD 4 ( + ) CD 25 ( + ) regulatory T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
However , in several murine models , depletion of CD 4 ( + ) CD 25 ( + ) regulatory T cells ( T ( regs ) ) causes a syndrome resembling human APS 2 with multiple endocrinopathies . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In this study we initially evaluated 43 patients with recent onset Type 1 diabetes ( disease duration < or= 6 months , 29 ICA positive ) with regard to beta cell secretion stimulation test with glucagon and immunologic parameters , including CD 4 + , CD 8 + , CD 4 + CD 25 + , CD 8 + CD 25 + lymphocyte subsets . ^^^ Relative size of CD 4 + CD 25 + subpopulation was 7 + / 5 . 4 % ( healthy control 2 + / 2 % , p < 0 . 001 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) T cells in these mice expressed higher levels of CTLA 4 and glucocorticoid induced TNF related receptor than naive CD 4 ( + ) T cells , but only 3 % of the recolonizing cells were CD 25 ( + ) and did not express significant foxP 3 mRNA . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The presence of autoreactive CD 4 ( + ) T cells in the peripheral blood of healthy human subjects was investigated after removal of CD 4 ( + ) CD 25 ( + ) regulatory T cells ( Treg ) . ^^^ Addition of CD 4 ( + ) CD 25 ( + ) Treg back to the CD 4 ( + ) CD 25 ( ) population suppressed the expansion of the autoreactive T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The relationship between Ag driven IL 10 secreting T ( Reg ) ( IL 10 T ( Reg ) ) and naturally occurring CD 4 ( + ) CD 25 ( + ) T ( Reg ) is as yet unclear . ^^^ We show that mouse IL 10 T ( Reg ) obtained using either in vitro or in vivo regimens of antigenic stimulation did not express the CD 4 ( + ) CD 25 ( + ) T ( Reg ) associated transcription factor Foxp 3 . ^^^ However , despite the absence of Foxp 3 expression , homogeneous populations of IL 10 T ( Reg ) inhibited the in vitro proliferation of CD 4 ( + ) CD 25 ( ) T cells with a similar efficiency to that of CD 4 ( + ) CD 25 ( + ) T ( Reg ) . ^^^ This inhibition of T cell proliferation by IL 10 T ( Reg ) was achieved through an IL 10 independent mechanism as seen for CD 4 ( + ) CD 25 ( + ) T ( Reg ) and was overcome by exogenous IL 2 . ^^^ Both IL 10 T ( Reg ) and CD 4 ( + ) CD 25 ( + ) T ( Reg ) were similar in that they produced little to no IL 2 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) T cells are critical mediators of peripheral immune tolerance . ^^^ To better understand how human CD 4 ( + ) CD 25 ( + ) T cells develop and function , we examined the diversity of CD 4 ( + ) CD 25 ( + ) and CD 4 ( + ) CD 25 ( ) T cell repertoires in both thymus and peripheral blood . ^^^ Levels of T receptor excision circles ( TREC ) were comparable in purified CD 4 ( + ) CD 25 ( + ) and CD 4 ( + ) CD 25 ( ) thymic populations , but were significantly higher than those in samples derived from peripheral blood , consistent with murine studies demonstrating thymic development of CD 4 ( + ) CD 25 ( + ) regulatory T cells . ^^^ Surprisingly , CD 4 ( + ) CD 25 ( ) T cells isolated from peripheral blood had greater TREC quantities than their CD 4 ( + ) CD 25 ( + ) counterparts , supporting the possibility of extrathymic expansion as well . ^^^ CD 4 ( + ) CD 25 ( + ) and CD 4 ( + ) CD 25 ( ) T cells from a given individual showed overlapping profiles with respect to diversity by Vbeta staining and spectratyping . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
This study investigates the role of CD 4 ( + ) CD 25 ( + ) regulatory T cells during the clinical course of juvenile idiopathic arthritis ( JIA ) . ^^^ Our data show that patients with pers OA JIA display a significantly higher frequency of CD 4 ( + ) CD 25 ( bright ) T cells with concomitant higher levels of mRNA FoxP 3 in the peripheral blood than ext OA JIA patients . ^^^ Furthermore , while numbers of synovial fluid ( SF ) CD 4 ( + ) CD 25 ( bright ) T cells were equal in both patient groups , pers OA JIA patients displayed a higher frequency of CD 4 ( + ) CD 25 ( int ) T cells and therefore of CD 4 ( + ) CD 25 ( total ) in the SF than ext OA JIA patients . ^^^ Analysis of FoxP 3 mRNA levels revealed a high expression in SF CD 4 ( + ) CD 25 ( bright ) T cells of both patient groups and also significant expression of FoxP 3 mRNA in the CD 4 ( + ) CD 25 ( int ) T cell population . ^^^ The CD 4 ( + ) CD 25 ( bright ) cells of both patient groups and the CD 4 ( + ) CD 25 ( int ) cells of pers OA JIA patients were able to suppress responses of CD 25 ( neg ) cells in vitro . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
RESULTS : The bronchiolitis obliterans group showed marked neutrophilia , high IL 8 and higher CD 4 ( + ) CD 25 ( + ) and CD 8 ( + ) CD 45 ( + ) bronchoalveolar lavage fluid levels when compared with their stable controls . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) Tregs is a new type of suppressor cell . ^^^ The population of CD 4 ( + ) , CD 8 ( + ) , CD 4 ( + ) CD 25 ( + ) , and CD 4 ( + ) CD 25 ( + ) CTLA 4 ( + ) T cells was evaluated by flow cytometry . ^^^ In the group of chemotherapy , although the CD4 / CD8 ratio ( 1 . 19+ / 0 . 3 ) was almost near to that of the healthy group , the total number of lymphocytes ( 0 . 8+ / 0 . 53 x 10 ( 9 ) ) was less than those of the other two groups , and the proportions of CD 4 ( + ) CD 25 ( + ) Tregs ( 20 . 3 % + / 4 . 1 % ) and CD 4 ( + ) CD 25 ( + ) CTLA 4 ( + ) T cells ( 11 . 5 % + / 2 . 2 % ) were higher than those of the other two groups . ^^^ CONCLUSION : The population of CD 4 ( + ) CD 25 ( + ) Tregs in peripheral blood of the patients with B NHL with or without chemotherapy was significantly higher than those in healthy donors , which may be one of the important reasons of immunosuppression in B NHL . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) regulatory T cells ( Tregs ) were isolated from peripheral blood and tested in functional assays . ^^^ Birch pollen IT did not lead to detectable changes in the number or function of the CD 4 ( + ) CD 25 ( + ) Tregs . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The frequencies of CD 4 ( + ) CD 25 ( + ) regulatory T cells in the spleen were significantly decreased in neonatal thymectomized mice administered with poly 1 : C compared to PBS treated neonatal thymectomized mice ( P < 0 . 01 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We have investigated CTLA 4 kinetics on human purified peripheral CD 4 ( + ) , nave , memory , CD 4 ( + ) CD 25 ( ) , CD 4 ( + ) CD 25 ( + ) regulatory T cells , and T cell clones . ^^^ Cell surface CTLA 4 expression was then investigated on CD 4 ( + ) CD45RA ( + ) ( nave ) , CD 4 ( + ) CD45RO ( + ) ( memory ) , CD 4 ( + ) CD 25 ( ) , and CD 4 ( + ) CD 25 ( + ) T cells . ^^^ On nave and CD 4 ( + ) CD 25 ( ) T cells , CTLA 4 expression declined after four hours . ^^^ In contrast , on memory and CD 4 ( + ) CD 25 ( + ) T cells , high levels of expression were maintained until at least 48 hours . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We measured oxidative markers , serum proinflammatory cytokines , soluble cytokine receptors and subsets of peripheral blood lymphocytes ( by varying combinations of CD 4 , CD 8 , CD 23 or low affinity IgE receptor , and CD 25 or IL 2 receptor ) from 38 type 1 patients , 76 low risk ( i . e . without underlying islet autoimmunity ) non diabetic first degree relatives of diabetic patients , and 95 healthy subjects . ^^^ In the whole study group , we found a correlation ( multiple R 0 . 5 , P < 0 . 001 ) of CD 23 ( + ) CD 25 ( + ) cells with blood counts of monocytes , CD 4 ( + ) CD 8 ( + ) cells , CD 25 ( + ) cells , basal haemolysis and plasma levels of thiols . ^^^ Our study reports the first evidence that the oxidative stress observed in type 1 families is related to immunological hallmarks ( decreased peripheral numbers of monocytes as well as cells bearing a CD 4 ( + ) CD 8 ( + ) , CD 23 ( + ) CD 25 ( + ) and CD 25 ( + ) phenotype ) from which the involvement of some immunoregulatory mechanisms could be suspected . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
OBJECTIVES : In the present study we investigated the number of T cell subsets ( CD 4 , CD 8 , CD45RO , CD45RA , CD 2 , CD 25 ) , cells expressing NK receptors ( CD 94 and CD 161 ) , the proliferation marker Ki 67 and the keratinization marker keratin ( K 10 ) across the margin of the spreading psoriatic plaque : distant uninvolved skin , the outer margin ( immediately outside the clinical edge ) , the inner margin ( immediately inside the clinical edge ) and the central area . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) T cells are potent immunoregulatory cells that suppress TCR induced proliferation of CD 4 and CD 8 T cells in vitro by a cell contact dependent mechanism . ^^^ Addition of IL 2 or anti CD 28 abrogates CD 4 ( + ) CD 25 ( + ) mediated suppression of proliferation and has been assumed to `` break suppression . ' ' We examined IL 2 mRNA by quantitative PCR in cocultures of mouse CD 4 ( + ) CD 25 ( + ) and CD 4 ( + ) CD 25 ( ) T cells . ^^^ These results are most compatible with a model in which CD 4 ( + ) CD 25 ( + ) T cells do not suppress the initial activation of CD 4 ( + ) CD 25 ( ) T cells , but mediate their suppressive effects following production of IL 2 by the responder cells resulting in both the expansion of the CD 4 ( + ) CD 25 ( + ) T cells and the induction of their suppressor function . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We used a TCR transgenic model of allograft rejection to characterize the in vivo activity of CD 4 ( + ) CD 25 ( + ) T reg . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Measures of activation included changes in CD 4 , CD 25 , CD 44 , CD45RB , CD62L , CD 69 , caspase 3 activation , CFSE dilution , size , number of cells recovered , and cytokine production . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The presence of increased frequencies of CD 4 ( + ) CD 25 ( + ) regulatory T cells in donor grafts has been shown to ameliorate GVHD after allogeneic transplantation in murine models . ^^^ To determine whether a similar relationship exists in humans , we quantitated the coexpression of CD 25 on CD 4 ( + ) and CD 8 ( + ) T cells within 60 donor grafts infused into matched siblings and examined GVHD incidence in the respective recipients . ^^^ Recipients in whom GVHD developed received donor grafts containing significantly higher frequencies of CD 4 ( + ) T cells coexpressing CD 25 than those who did not ( median , 9 . 26 % vs 2 . 22 % ; P = . 004 ) . ^^^ Furthermore , transplant recipients who received grafts containing fewer CD 4 ( + ) CD 25 ( + ) and CD 8 ( + ) CD 25 ( + ) T cells were less likely to acquire acute GVHD , even though these donor recipient pairs were similar to others with respect to relevant clinical variables . ^^^ These data suggest that the coexpression of CD 4 and CD 25 may be insufficient to identify regulatory T cells in humans and that increased frequencies and numbers of CD 25 ( + ) T cells in donor grafts is associated with GVHD in transplant recipients . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Lipopolysaccharide increased expression of cells positive for both CD 3 and TLR 4 ; both TLR 4 and interleukin 10 ; and both CD 4 and CD 25 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Flow cytometry was used to determine the percentages of total T lymphocytes , B lymphocytes , helper / inducer T cells , suppressor / cytotoxic T cells , natural killer ( NK ) cells , activated T cells and interleukin 2 receptor ( IL 2R ) with the use of CD 3 , CD 19 , CD 4 , CD 8 , CD 16 , HLA DR and CD 25 monoclonal antibodies , respectively . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Immune responses to retinal self antigens in CD 25 ( + ) CD 4 ( + ) regulatory T cell depleted mice . ^^^ PURPOSE : Prior work has shown that autoimmune uveoretinitis develops spontaneously in CD 25 ( + ) CD 4 ( + ) regulatory T cell depleted mice ( Tr depleted mice ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Upon coculture with autologous lymphocytes , mDC activated the autologous T cells as reflected by increased CD 25 and cytotoxic T lymphocyte antigen 4 expression on CD 4 ( + ) T cells together with the increased production of both T helper 1 ( Th 1 ) ( IL 2 and interferon gamma ) and Th 2 ( IL 10 and IL 4 ) cytokines . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Moreover , IL 10 neutralization and CD 4 ( + ) CD 25 ( + ) T cell depletion synergistically act to dramatically increase HIV p 24 specific CD 4 T cell responses induced by IFN DCs ( 31 . 7 fold increase ) but not responses induced by IL 4 DCs . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
This finding correlates with the decreased numbers of CD 4 ( + ) CD 25 ( + ) T regulatory ( T ( R ) ) cells in the islets and pancreatic lymph nodes , in comparison to disease protected CD 154 sufficient mice . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
METHODS : In two follow up surveys of the SARS patients discharged from our hospital , the changes of CD 3 ( + ) , CD 4 ( + ) , CD 8 ( + ) , CD 8 ( + ) CD 28 ( + ) , CD 8 ( + ) CD 28 ( ) , CD 3 ( + ) CD 25 ( + ) , CD 3 ( + ) CD 69 ( + ) , CD 3 ( + ) HLA DR ( + ) T cells and the ratio of CD 4 ( + ) / CD8 ( + ) T cells in peripheral blood of SARS patients treated unitedly with Chinese and Western medicines were detected by flow cytometry . ^^^ As for the percentages of CD 3 ( + ) , CD 8 ( + ) , CD 4 ( + ) / CD8 ( + ) , CD 3 ( + ) CD 25 ( + ) , CD 3 ( + ) CD 69 ( + ) and CD 3 ( + ) HLA DR ( + ) T cells , there was marked difference between 1st and 2nd follow up results , but for other molecules there was no notable difference . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Children who outgrew their allergy ( tolerant children ) had higher frequencies of circulating CD 4 ( + ) CD 25 ( + ) T cells and decreased in vitro proliferative responses to bovine beta lactoglobulin in peripheral blood mononuclear cells ( PBMCs ) compared with children who maintained clinically active allergy . ^^^ This suggests that tolerance is associated with the appearance of circulating CD 4 ( + ) CD 25 ( + ) regulatory T ( Treg ) cells that are capable of suppressing the effector T cells generated 1 wk after reintroduction of cow ' s milk . ^^^ The suppressive function of the CD 4 ( + ) CD 25 ( + ) Treg cells was shown to be partly cell contact dependent . ^^^ Collectively , our study provides human data to suggest that mucosal induction of tolerance against dietary antigens is associated with the development of CD 4 ( + ) CD 25 ( + ) Treg cells . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Adult T cell leukaemia / lymphoma ( ATLL ) is an aggressive neoplastic disease that usually exhibits a CD 4 ( + ) CD 25 ( + ) phenotype . ^^^ Regulatory T cells ( Treg ) , which suppress T cell effector function , are characterized by the co expression of CD 4 and CD 25 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
These IL 10 producing cells might be so called regulatory T cells and appear to be identified by the CD 4 ( + ) CD 25 ( + ) phenotype . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
RENCA implantation reduced the proportion of CD 4 ( + ) cells among splenocytes , whereas anti CD 25 mAb treatment increased it . ^^^ Both CD 4 ( + ) and CD 8 ( + ) cells were stained around the remnant microscopic RENCA tumour after anti CD 25 mAb treatment . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
VDR ligands can induce in vitro and in vivo tolerogenic DCs able to enhance CD 4 ( + ) CD 25 ( + ) suppressor T cells that , in turn , inhibit Th 1 cell responses . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Here , we studied the effect on the induction of EAE of depleting antibodies to CD 4 , CD 8 , or CD 25 administered before either the first or the second dose of MOG p 35 55 . ^^^ These findings are compatible with the idea that CD 4 T cells are required for the initial induction of EAE and that the disease is down regulated by T cells expressing CD 8 or CD 25 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) regulatory T cells from these two mice strains were fully competent and do not explain the difference in disease susceptibility . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
These findings suggest that activated CD 4 ( + ) T cells ( characterized by a high CD 25 expression ) might contribute to the tolerogenic immune response toward an antigen , such as cow ' s milk , through the production of IL 10 . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
A leaky block in the differentiation of CD 4 ( ) CD 8 ( ) CD 3 ( ) CD 44 ( ) CD 25 ( + ) ( TN 3 ) into CD 4 ( ) CD 8 ( ) CD 3 ( ) CD 44 ( ) CD 25 ( ) ( TN 4 ) cells was observed . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The present study demonstrates that CD 4 ( + ) CD 25 ( + ) T cells , expanded in peripheral blood of HIV infected patients receiving highly active antiretroviral therapy ( HAART ) , exhibit phenotypic , molecular , and functional characteristics of regulatory T cells . ^^^ The majority of peripheral CD 4 ( + ) CD 25 ( + ) T cells from HIV infected patients expressed a memory phenotype . ^^^ CD 4 ( + ) CD 25 ( + ) T cells weakly proliferated to immobilized anti CD 3 monoclonal antibody ( mAb ) and addition of soluble anti CD 28 mAb significantly increased proliferation . ^^^ In contrast to CD 4 ( + ) CD 25 ( ) T cells , CD 4 ( + ) CD 25 ( + ) T cells from HIV infected patients did not proliferate in response to recall antigens and to p 24 protein . ^^^ The proliferative capacity of CD 4 T cells to tuberculin , cytomegalovirus ( CMV ) , and p 24 significantly increased following depletion of CD 4 ( + ) CD 25 ( + ) T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Although central and peripheral tolerance are important for the regulation of human immune responses to self and microbial antigens , an important role of suppressor CD 4 ( + ) CD 25 ( + ) T cells is suggested from the recent investigations of human autoimmune diseases and HIV . ^^^ These new data provide increasing evidence that altered function of CD 4 ( + ) CD 25 ( + ) T cells may be an important factor in a wide range of human inflammatory and infectious diseases . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 25 ( + ) CD 4 ( + ) regulatory T cells from the peripheral blood of asymptomatic HIV infected individuals regulate CD 4 ( + ) and CD 8 ( + ) HIV specific T cell immune responses in vitro and are associated with favorable clinical markers of disease status . ^^^ The potential role of CD 25 ( + ) CD 4 ( + ) regulatory T cells ( T reg cells ) , previously shown to inhibit immune responses directed against numerous pathogens , as suppressors of HIV specific T cell responses was investigated . ^^^ In the majority of healthy HIV infected individuals , CD 25 ( + ) CD 4 ( + ) T cells significantly suppressed cellular proliferation and cytokine production by CD 4 ( + ) and CD 8 ( + ) T cells in response to HIV antigens / peptides in vitro ; these effects were cell contact dependent and IL 10 and TGF beta independent . ^^^ Individuals with strong HIV specific CD 25 ( + ) T reg cell function in vitro had significantly lower levels of plasma viremia and higher CD 4 ( + ) : CD 8 ( + ) T cell ratios than did those individuals in whom this activity could not be detected . ^^^ These in vitro data suggest that CD 25 ( + ) CD 4 ( + ) T reg cells may contribute to the diminution of HIV specific T cell immune responses in vivo in the early stages of HIV disease . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Although alpha ( E ) beta ( 7 ) ( + ) CD 4 ( + ) T cells possess a regulatory phenotype ( CD 25 ( + ) , L selectin ( lo ) , and CD45RB ( lo ) ) , express IL 10 , and suppress effector T cell proliferation in vitro , they can not prevent ileitis development in SCID mice adoptively transferred with effector CD 4 ( + ) T cells , although the CD 4 ( + ) CD 25 ( + ) subset , which overlaps with the alpha ( E ) beta ( 7 ) ( + ) CD 4 ( + ) subset , prevents colitis . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Primed by adult host DCs , CB and adult blood CD 4 ( + ) T cells underwent similar changes in the expression of CD45RA / 45RO , CD 25 , CD40L and CTLA 4 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In particular , the disappearance of the CD 4 ( + ) CD 25 ( + ) T cell population , which possibly contains a subpopulation of regulatory T cells , may contribute to the initiation of labor . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) immune regulatory T cells ( Tregs ) , long recognized for their critical role in induction and maintenance of self tolerance and prevention of autoimmunity , are also important in the regulation of immune responses in allogeneic bone marrow ( BM ) and solid organ transplantation . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
This brief review describes the types of interactions that occur between CD 4 ( + ) CD 25 ( + ) regulatory T cells ( Treg ) and microbial pathogens . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
More recent studies reveal that costimulatory molecules enhance clonal deletion of autoreactive T cells as well as generation and homeostasis of the CD 25 ( + ) CD 4 ( + ) regulatory T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The thymus derived CD 4 ( + ) CD 25 ( + ) T cells belong to a subset of regulatory T cells potentially capable of suppressing the proliferation of pathogenic effector T cells . ^^^ In this study , we find that the 4 1BB costimulator receptor , best known for promoting the proliferation and survival of CD 8 ( + ) T cells , also induces the proliferation of the CD 4 ( + ) CD 25 ( + ) regulatory T cells both in culture and in vivo . ^^^ The proliferating CD 4 ( + ) CD 25 ( + ) T cells produce no detectable IL 2 , suggesting that 4 1BB costimulation of these cells does not involve IL 2 production . ^^^ The 4 1BB expanded CD 4 ( + ) CD 25 ( + ) T cells are functional , as they remain suppressive to other T cells in coculture . ^^^ These results support the notion that the peripheral expansion of the CD 4 ( + ) CD 25 ( + ) T cells is controlled in part by costimulation . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In this study , we report that targeted CTLA 4 engagement can induce immune tolerance to a specific target through selective induction of an Ag specific CD 4 ( + ) CD 25 ( + ) CTLA 4 ( high ) regulatory T cell ( Treg cell ) population . ^^^ Although rechallenge with alloantigen failed to break the unresponsiveness , a transient recovery from tolerance was observed in the presence of high concentrations of exogenous IL 2 , saturating concentrations of neutralizing anti TGF beta 1 and anti IL 10 Abs , and blocking anti CTLA 4 Ab , and upon depletion of CD 4 ( + ) CD 25 ( + ) Treg cells . ^^^ The CD 4 ( + ) CD 25 ( + ) CTLA 4 ( high ) Treg cells from tolerant mice suppressed the effector function of CD 25 ( ) T cells from Ag primed mice . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Antigen specific CD 8 ( + ) CD 28 ( ) and CD 4 ( + ) CD 25 ( + ) T suppressor / regulatory cells induce the upregulation of inhibitory receptors expressed by antigen presenting cells ( APC ) belonging to the family of immunoglobulin like transcripts ( ILTs ) and downregulation of costimulatory molecules in APC . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
There are reports suggesting an influence of CD 4 ( + ) CD 25 ( + ) T regulatory cells ( Treg ) on cytotoxic lymphocytes . ^^^ Inhibition of the cytotoxic reactions in the presence of Treg cells was found to be associated with the generation of conglomerates formed by CD 4 ( + ) CD 25 ( + ) and the cytotoxic cells , as observed under the fluorescence microscope . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
BACKGROUND : CD 4 ( + ) CD 25 ( + ) regulatory T cells are key controllers of peripheral immunological self tolerance and suppress various autoimmune diseases in animal models , but few studies have been done to define their roles in myasthenia gravis ( MG ) so far . ^^^ OBJECTIVE : To investigate frequencies and dynamic changes of blood CD 4 ( + ) CD 25 ( + ) T cells from MG patients . ^^^ METHODS : The peripheral blood CD 4 ( + ) CD 25 ( + ) T cells of 29 MG patients and 23 healthy controls were detected by three color flow cytometry . ^^^ RESULTS : Myasthenic patients with symptomatically uncontrollable disease showed slightly lower percentages of CD 4 ( + ) CD 25 ( + ) T cells ( mean = 3 . 79 + / 1 . 40 % ; P = 0 . 12 ) , whereas MG patients with clinically stable disease had significantly increased CD 4 ( + ) CD 25 ( + ) T cells ( mean = 8 . 45 + / 1 . 96 % , P = 0 . 0001 ) , as compared with healthy controls ( mean = 4 . 53 + / 0 . 96 % ) . ^^^ In addition , thymectomized MG patients had significantly higher percentages of CD 4 ( + ) CD 25 ( + ) T cells ( mean = 8 . 44 + / 2 . 39 % ) , as compared with both non thymectomized MG patients ( mean = 5 . 88 + / 2 . 89 % , P = 0 . 038 ) and healthy controls ( P = 0 . 003 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The active suppression is mediated by the CD 4 ( + ) CD 25 ( + ) immunoregulatory cells and is associated with the down regulation of Th 1 type cytokines and the up regulation of the secretion of IL 10 and the immunosuppressive cytokine , transforming growth factor beta . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Finally , exposure to WDDD decreased both the percentage and absolute number of regulatory CD 4 ( + ) CD 25 ( + ) T cells in the spleen , which was consistent with a significant increase in interferon gamma ( IFN gamma ) production from Con A stimulated splenocytes . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The Id 1 transgenic thymus is characterized by an accumulation of CD 4 CD 8 CD44+ CD 25 thymocytes , a dramatic reduction of CD4+ CD8+ thymocytes , and an abundance of apoptotic cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The following markers were investigated in skin biopsies taken from the aforementioned patients by quantitative immunohistochemistry : CD 2 , CD 4 , CD 8 , CD 25 , CD 161 , CD 94 , CD45RO , CD45RA , HLA DR , Keratin 10 , Ki 67 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
A notable finding was the appearance in these cultures of CD 4 ( + ) CD 25 ( + ) CTLA 4 ( + ) T cells that de novo develop MLR suppressive activity . ^^^ CFSE labeling studies indicate that the CD 4 ( + ) CD 25 ( + ) cells observed at the end of the culture period were present in the day 0 starting populations , but they were not suppressive in MLR responses . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In this study , we demonstrate that the CD 4 ( + ) CD 25 ( bright ) T cells reside in the human intestinal lamina propria ( LP ) and functionally retain regulatory activities . ^^^ All human LP CD 4 ( + ) T cells regardless of CD 25 expression constitutively expressed CTLA 4 , glucocorticoid induced TNFR family related protein , and Foxp 3 and proliferate poorly . ^^^ Although LP CD 4 ( + ) CD 25 ( ) T cells showed an activated and anergic / memory phenotype , they did not retain regulatory activity . ^^^ In LP CD 4 ( + ) CD 25 ( + ) T cells , however , cells expressing CD 25 at high levels ( CD 4 ( + ) CD 25 ( bright ) ) suppressed the proliferation and various cytokine productions of CD 4 ( + ) CD 25 ( ) T cells . ^^^ LP CD 4 ( + ) CD 25 ( bright ) T cells by themselves produced fewer amounts of IL 2 , IFN gamma , and IL 10 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
This was associated with an accumulation of CD 4 ( + ) T bet ( + ) CD 25 ( + ) effector Th 1 cells in the lamina propria of colitic mice . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Using MHC class 2 dimers loaded with tolerogenic peptide , we found that pCons favored expansion of peptide reactive CD 4 ( + ) CD 25 ( + ) regulatory T cells ( T ( R ) ) that inhibited in vitro production of anti dsDNA Ab forming cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In this review , we summarize current efforts to induce and maintain tolerance in the autoimmune diabetes setting by using therapeutic vaccination with CD 4 ( + ) CD 25 ( + ) regulatory T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
This was followed by an imbalance between CD 4 ( + ) CD 25 ( + ) and CD 4 ( + ) CD 69 ( + ) T cells in the pancreas that was mirrored in the phenotype of autoreactive T cells in the blood . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In murine models , freshly isolated or ex vivo expanded CD 4 ( + ) CD 25 ( high ) regulatory T cells ( Treg ) are able to ameliorate GVHD while maintaining graft versus leukaemia reactions . ^^^ In this study , we prospectively quantified CD 4 ( + ) CD 25 ( high ) and activated CD 134 ( + ) lymphocytes in 119 peripheral blood samples from 35 consecutive patients who underwent allogeneic bone marrow transplantation ( BMT ) . ^^^ Fifty five samples obtained less than 100 d after allogeneic BMT , were not statistically different regarding CD 4 ( + ) CD 25 ( high ) Treg or CD 134 ( + ) lymphocytes compared with those obtained from patients with ( n = 35 ) or without ( n = 20 ) acute GVHD . ^^^ Our findings suggest that the suppressive activity of CD 4 ( + ) CD 25 ( high ) Treg could be abrogated in vivo during cGVHD by CD 134 expression in a much higher number of activated donor T lymphocytes . ^^^ In addition to CD 4 ( + ) CD 25 ( high ) ex vivo expansion protocols , OX 40 blocking might be crucial to optimize the use of Treg to prevent GVHD . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Here we show that the ability to spontaneously manifest a T cell dependent protective response is restricted by naturally occurring CD 4 ( + ) CD 25 ( + ) regulatory T cells ( Treg ) ; depletion of Treg was beneficial in two mouse strains ( C57BL / 6J and BALB / c / OLA ) differing in their spontaneous T cell dependent ability to withstand the consequences of optic nerve injury . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The decline in the CTL response was associated by a concomitant expansion of CD 4 ( + ) CD 25 ( + ) T cells . ^^^ Triple color flow cytometric analyses revealed cytoplasmic IL 10 in the CD 4 ( + ) CD 25 ( + ) T cell fraction and the number of CD 4 ( + ) CD 25 ( + ) IL 10 ( + ) T cells were found to increase significantly in postvaccine PBL . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We first show that diabetes onset is prevented in such mice by infusion of polyclonal CD 4 ( + ) T cells expressing L selectin ( CD62L ) but not prevented or only marginally prevented by CD 4 ( + ) CD 25 ( + ) T cells . ^^^ Similarly , we found with a cotransfer model that CD 4 ( + ) CD62L ( + ) T cells but not CD 4 ( + ) CD 25 ( + ) T cells inhibited diabetes transfer into NOD SCID recipients by transgenic NOD BDC2 . 5 SCID cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Here we show , in detailed studies of CD 4 ( + ) CD 25 ( + ) FOXP 3 ( + ) T ( reg ) cells in 104 individuals affected with ovarian carcinoma , that human tumor T ( reg ) cells suppress tumor specific T cell immunity and contribute to growth of human tumors in vivo . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate whether the CD 25 + CD 4 + regulatory T cell population , which plays important roles not only in maintaining immunologic self tolerance but also in controlling the magnitude and character of antimicrobial immune responses , is related to the pathophysiology of Kawasaki disease ( KD ) . ^^^ We analyzed CD 25 + CD 4 + cells and the mRNA expression of Foxp 3 , cytotoxic T lymphocyte associated antigen 4 ( CTLA 4 ) , glucocorticoid induced tumor necrosis factor receptor ( GITR ) , and transforming growth factor beta in peripheral blood mononuclear cells and purified CD 4 + T cells . ^^^ RESULTS : The proportions of CD 25 + CD 4 + cells in patients with acute phase KD ( median , 2 . 35 % of total lymphocytes ) were significantly lower than those in healthy control subjects ( median , 3 . 14 % ) and control subjects with disease ( median , 3 . 15 % ) . ^^^ CONCLUSIONS : The decrease of CD 25 + CD 4 + regulatory T cells in the acute phase might have a role in the development of KD . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
However , the main non redundant activity of this cytokine centres on the regulation of T cell tolerance , and recent studies indicate that a failure in the production of CD 4 ( + ) CD 25 ( + ) regulatory T cells is the underlying cause of autoimmunity in the absence of IL 2 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Cat allergen peptide immunotherapy reduces CD 4 ( + ) T cell responses to cat allergen but does not alter suppression by CD 4 ( + ) CD 25 ( + ) T cells : a double blind placebo controlled study . ^^^ BACKGROUND : We have previously described both modification of allergen immunotherapy using peptide fragments , and reduced regulation of allergen stimulated T cells by CD 4 ( + ) CD 25 ( + ) T cells from allergic donors when compared with nonallergic controls . ^^^ It has been suggested that allergen immunotherapy induces regulatory T cell activity : we hypothesized that allergen peptide immunotherapy might increase suppressive activity of CD 4 ( + ) CD 25 ( + ) T cells . ^^^ OBJECTIVE : To examine cat allergen stimulated CD 4 T cell responses and their suppression by CD 4 ( + ) CD 25 ( + ) T cells before and after cat allergen peptide immunotherapy in a double blind placebo controlled study . ^^^ CD 4 ( + ) and CD 4 ( + ) CD 25 ( + ) were then isolated by immunomagnetic beads and cultured with allergen in vitro . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( ) CD 8 ( ) CD 25 ( ) CD44+ fetal thymocytes retained the activity to specifically re enter the thymus . ^^^ Among the chemokines produced by the fetal thymus , CCL 21 , CCL 25 , and CXCL 12 could attract CD 4 ( ) CD 8 ( ) CD 25 ( ) CD44+ fetal thymocytes . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
TGF beta induces Foxp 3 + T regulatory cells from CD 4 + CD 25 precursors . ^^^ CD 4 + CD 25 + regulatory T cells ( Tregs ) are potent suppressors , playing important roles in autoimmunity and transplantation tolerance . ^^^ Recent reports suggest a role for TGF beta in the generation of Tregs from CD 4 + CD 25 precursors , but the mechanism remains unknown . ^^^ Here , we demonstrate that TGF beta 2 triggers Foxp 3 expression in CD 4 + CD 25 precursors , and these Foxp 3 + cells act like conventional Tregs . ^^^ Thus , TGF beta is a key regulator of the signaling pathways that initiate and maintain Foxp 3 expression and suppressive function in CD 4 + CD 25 precursors . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In addition to intrinsic mechanisms regulating T cell quiescence , CD 4 + CD 25 + regulatory T cells ( Treg ) naturally arising in the thymus , engage in the maintenance of immunological self tolerance by preventing autoimmunity in vivo in a non cell autonomous manner . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
RESULTS : Deletion of CD 4 or CD 25 cells during costimulation blockade induced rapid rejection of skin but only minimally shortened islet allograft survival . ^^^ Deletion of CD 4 or CD 25 cells had no effect upon survival of healed in islet allografts , and CD 25 cell deletion had no effect upon healed in skin allograft survival . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Cord blood CD 4 ( + ) CD 25 ( + ) derived T regulatory cell lines express FoxP 3 protein and manifest potent suppressor function . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
RAG 1 ( / ) mice adoptively transferred with CD 8 ( + ) and CD 4 ( + ) T cells lacking the CD 4 ( + ) CD 25 ( + ) compartment mounted robust concomitant immunity , which was suppressed by readdition of CD 4 ( + ) CD 25 ( + ) cells . ^^^ Naturally occurring CD 4 ( + ) CD 25 ( + ) T cells efficiently suppressed concomitant immunity mediated by previously activated CD 8 ( + ) T cells , demonstrating that precursor regulatory T cells in naive hosts give rise to effective suppressors . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Engineered DC promoted an increased prevalence of CD 4 ( + ) CD 25 ( + ) T cells in NOD recipients at all ages examined and diabetes free recipients exhibited significantly greater numbers of CD 4 ( + ) CD 25 ( + ) T cells compared with untreated NOD mice . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In this study , we describe that the most immature intrathymic progenitors ( CD 25 ( ) / CD44 ( + ) / FcR ( + ) ) give rise to CD 4 ( + ) macrophages by oncostatin M responsive thymic epithelial cells ( ORTEC ) in an IL 7 dependent manner . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Treatment of NOD mice with a mitogenic anti CD 3 depletes CD 4 ( + ) CD 25 ( ) CD62L ( + ) but not CD 4 ( + ) CD 25 ( + ) CD62L ( + ) T cells , thereby resulting in an increase of the latter subset in the spleen . ^^^ These results demonstrate that the capacity of anti CD 3 to protect NOD mice from T1D correlates with its ability to perturb T cell homeostasis by inducing CD 8 ( + ) T cell AICD and increasing the number of CD 4 ( + ) CD 25 ( + ) CD62L ( + ) T cells in the periphery . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Here we show that type B CpG ODN effectively promotes PDCs to prime allogeneic naive CD 4 ( + ) CD 25 ( ) T cells to differentiate into CD 4 ( + ) CD 25 ( + ) regulatory T ( Treg ) cells . ^^^ The CD 4 ( + ) CD 25 ( + ) T cells induced by CpG ODN activated PDCs express forkhead transcription factor 3 and produce IL 10 , TGF beta , IFN gamma , and IL 6 , but low IL 2 and IL 4 . ^^^ These CD 4 ( + ) CD 25 ( + ) T cells are hyporesponsive to secondary alloantigen stimulation and strongly inhibit proliferation of autologous or allogeneic naive CD 4 ( + ) T cells in an Ag nonspecific manner . ^^^ CpG ODN activated PDCs require direct contact with T cells to induce CD 4 ( + ) CD 25 ( + ) Treg cells . ^^^ Both CpG A and CpG C ODN activated PDCs similarly induced the generation of CD 4 ( + ) CD 25 ( + ) Treg cells with strong immune suppressive function . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) T cells are an important population that plays a crucial role in the maintenance of peripheral self tolerance . ^^^ The elimination of CD 25 ( + ) T cells did not induce vitiligo like coat color changes , though that of CD 4 ( + ) T cells induced the change in 60 % of mice . ^^^ Furthermore , we confirmed that elimination of CD 25 ( + ) T cells did not affect the T helper ( Th ) 1 / Th2 cytokine profile , while that of CD 4 ( + ) T cells abrogated the Th 2 cytokines ( IL 4 and IL 10 ) and resulted in a Th 1 dominant cytokine profile in the tumor draining lymph nodes ( TDLNs ) of B16 / IL 12 bearing mice . ^^^ These results indicate that in vivo depletion of CD 25 ( + ) regulatory T cells is a potent useful adjuvant in immunotherapy of B16F10 melanoma , when combined with IL 12 gene transfer and that the enhancement of the antitumor effect by CD 25 ( + ) T cell depletion is mediated through CD 8 ( + ) T cells and may differ from the enhancing mechanism caused by CD 4 ( + ) T cell depletion . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We describe here a population of T ( R ) cells that developed in vivo from naive CD 4 ( + ) CD 25 ( ) T cells during a T helper type 1 ( T ( H ) 1 ) polarized response , distinct from CD 25 ( + ) T ( R ) cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Gene modified T cells that can selectively be depleted and CD 4 ( + ) CD 25 ( + ) regulatory T cells are under investigation for their ability to modulate alloreactivity after HSCT . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
At 18 days after plasmid administration , GHRH treated animals had increased numbers of CD 2 ( + ) alphabeta T cells ( P < 0 . 004 ) , CD 25 ( + ) CD 4 ( + ) cells ( P < 0 . 0007 ) , and CD 4 ( + ) CD45R ( + ) cells ( P < 0 . 016 ) compared to controls . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We show that 3 day old mice already contain a significant compartment of Foxp 3 expressing CD 25 ( + ) CD 4 ( + ) splenocytes . ^^^ Whereas , in adult spleen , the subsets of regulatory T cells ( CD 25 ( + ) and / or CD 103 ( + ) ) express high amounts of Foxp 3 mRNA , in 3 day old mice , both thymic and splenic CD 25 ( + ) CD 4 ( + ) T cell subsets express lower amounts of Foxp 3 mRNA , and CD 103 ( + ) cells are barely detected . ^^^ In adult day 3 thymectomized mice , the CD 25 ( + ) CD 4 ( + ) T cell subset is overrepresented ( most of the cells being CD 103 ( + ) ) and expresses high amounts of Foxp 3 mRNA , independent of the development of autoimmune gastritis . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Microglial cells from brain were isolated and assessed through E rosette formation , SEM and FACS for CD 2 , MHC class 2 , CD 25 , and CD 4 . ^^^ A general trend of highest receptor saturation and microglial activation , measured through the activation marker CD 25 and CD 4 expression , was observed in 2nd dose of T11TS administration , which was then dampened via a complex immune feedback mechanism in the 3rd dose . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) regulatory T cells in rheumatoid arthritis : differences in the presence , phenotype , and function between peripheral blood and synovial fluid . ^^^ OBJECTIVE : In mice , CD 4 ( + ) CD 25 ( + ) regulatory T cells play a pivotal role in preventing autoimmunity . ^^^ We investigated the presence , phenotype , and function of CD 4 ( + ) CD 25 ( + ) regulatory T cells in peripheral blood ( PB ) and synovial fluid ( SF ) from patients with rheumatoid arthritis ( RA ) . ^^^ METHODS : The presence and phenotype of CD 4 ( + ) CD 25 ( + ) regulatory T cells were determined by flow cytometry . ^^^ Anergy and suppressive activity were assessed by culturing CD 4 ( + ) CD 25 ( ) and CD 4 ( + ) CD 25 ( + ) T cells with anti CD 3 monoclonal antibodies and antigen presenting cells , followed by proliferation and cytokine detection . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Naturally occurring CD 25 ( + ) CD 4 ( + ) regulatory T cells are engaged in the maintenance of immunological self tolerance and down regulation of various immune responses . ^^^ Recent studies with mice showed that Foxp 3 , which encodes the transcription factor Scurfin , is a master regulatory gene for the development and function of CD 25 ( + ) CD 4 ( + ) regulatory T cells . ^^^ Here we examined the role of FOXP 3 in human CD 25 ( + ) CD 4 ( + ) regulatory T cells . ^^^ The FOXP 3 gene and its protein product were preferentially expressed in peripheral CD 25 ( + ) CD 4 ( + ) T cells , in particular CD 25 ( + ) CD45RO ( + ) CD 4 ( + ) T cells in normal individuals and , interestingly , in some human T cell leukemia virus type 1 infected T cell lines , which constitutively express CD 25 . ^^^ TCR stimulation of CD 25 ( ) CD45RO ( ) CD 4 ( + ) naive T cells failed to elicit FOXP 3 expression at the gene or protein level . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Cord blood samples were collected for organochlorine and mercury analyses and also to isolate cord blood mononuclear cells ( CBMCs ) for the in vitro assessment of cytokine production and expression of surface markers after mitogenic stimulation ( CD 4 ( + ) CD45RO ( + ) , CD 8 ( + ) CD45RO ( + ) , CD 3 ( + ) CD 25 ( + ) , and CD 8 ( + ) HLA DR ( + ) ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In addition , Tr 1 cells induced by iDCs do not require the presence of CD 4 ( + ) CD 25 ( + ) Tr cells for their generation , nor do they express high constitutive levels of CD 25 or the transcription factor FoxP 3 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
It has been demonstrated that T cells with regulatory properties are present within the peripheral blood CD 4 ( + ) CD 25 ( + ) T cell compartment . ^^^ Here , we describe an original method to purify human CD 4 ( + ) CD 25 ( + ) CD 152 ( + ) T lymphocytes as living cells by forcing the exportation of CTLA 4 molecules stored in intracellular vesicules at the cell surface . ^^^ By doing so , we demonstrate that CD 4 ( + ) CD 25 ( + ) T cells contain a smaller and more homogeneous population enriched in cells with in vitro regulatory activity . ^^^ Moreover , we show that this enrichment in regulatory T cells is associated with an increased expression of Foxp 3 and that CD 4 ( + ) CD 25 ( + ) CD 152 ( + ) T lymphocytes display a much stronger suppressive activity in controlling in vitro proliferation of alloantigen specific T cells than CD 4 ( + ) CD 25 ( + ) CD 152 ( ) T lymphocytes purified in parallel . ^^^ Lastly , by purifying such cells expressing CTLA 4 , we demonstrate that indeed CTLA 4 is involved in CD 4 ( + ) CD 25 ( + ) CD 152 ( + ) T cell regulatory activity , while suppressive cytokines are not . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Natural CD 4 ( + ) CD 25 ( + ) Tregs express LAG 3 upon activation , which is significantly enhanced in the presence of effector cells , whereas CD 4 ( + ) CD 25 ( + ) Tregs from LAG 3 ( / ) mice exhibit reduced regulatory activity . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Here , we demonstrate that activated human CD 4 ( + ) CD 25 ( + ) natural Treg cells express granzyme A but very little granzyme B . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
An immunomodulatory role for CD 4 ( + ) CD 25 ( + ) regulatory T lymphocytes in hepatitis C virus infection . ^^^ The CD 4 ( + ) CD 25 ( + ) regulatory T lymphocytes have been implicated in suppressing T cell immune responses . ^^^ Our aim was to characterize the frequency , phenotype , function , and specificity of CD 4 ( + ) CD 25 ( + ) T cells in hepatitis C virus ( HCV ) infection . ^^^ Peripheral CD 4 ( + ) CD 25 ( + ) cells from recovered ( n = 15 ) , chronic infected ( n = 30 ) , and normal control ( n = 15 ) subjects were analyzed ex vivo for quantitation , phenotype , and effect on HCV specific interferon gamma production and proliferation . ^^^ CD 4 ( + ) CD 25 ( + ) specificity was determined by intracellular cytokine staining for interleukin 10 ( IL 10 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Although unfractionated CD 4 ( + ) T cells have not been shown to be an efficient facilitating population , CD 4 ( + ) CD 25 ( + ) regulatory cells ( T reg ' s ) were examined for their capacity to support allogeneic hematopoietic engraftment . ^^^ Donor CD 4 ( + ) CD 25 ( + ) T cells may therefore represent a potential alternative to unfractionated T cells for promotion of allogeneic engraftment in clinical hematopoietic cell transplantation . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Anti CD 3 stimulation of reconstituted T cells showed ' mean ' levels of CD 4 and CD 25 were enhanced by 34 . 5 and 31 . 1 % in immunized mice , which was comparable to 53 . 2 and 50 . 7 % , respectively , in challenged immunized mice , and were dominant over CD8+ T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Signaling through the pre TCR controls the differentiation of immature CD 4 ( ) CD 8 ( ) CD 25 ( + ) CD 44 ( ) [ double negative ( DN ) 3 ] thymocytes into CD 4 ( + ) CD 8 ( + ) double positive ( DP ) cells through the CD 4 ( ) CD 8 ( ) CD 25 ( ) CD 44 ( ) ( DN 4 ) stage . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
BAY 50 4798 induced dose dependent increases in CD 25 expression and proliferation of T cells , NK , and B cells and showed selectivity for CD 4 T cells expressing CD 25 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Concentration of soluble CD 4 , CD 8 , CD 25 receptors as well IFN gamma and IL 4 released by lymphocyte of chronic Lyme patients cultured with 3 genotypes of Borrelia burgdorferi ] . ^^^ The increase in sCD 4 , sCD 8 and sCD 25 concentrations indicates the activation of these lymphocytes with CD 4 , CD 8 , CD 25 receptors in this group of patients . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Histology ( elastin ) and immunohistochemistry using monoclonal antibodies to CD 4 , CD 8 , CD45R , RT1B , CD11b / c , CD 25 , and alpha actin was performed to examine the epicardial and intramyocardial coronary arteries . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) regulatory T cells ( Tregs ) mediate peripheral T cell homeostasis and contribute to self tolerance . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The neurodegeneration was attenuated or augmented by elimination or addition , respectively , of naturally occurring CD 4 ( + ) CD 25 ( + ) regulatory T cells ( Treg ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Regulatory T ( Treg ) cells , derived from co cultures of unfractionated CD 4 ( + ) T cells and immature dendritic cells ( DC ) , suppress enteroantigen induced proliferation of CD 4 ( + ) CD 25 ( ) T cells . ^^^ The DC induced Treg cells , and hereof purified CD 25 ( + ) and CD 25 ( ) T cell fractions , were co injected into severe combined immunodeficiency ( SCID ) mice with colitis inducing CD 4 ( + ) CD 25 ( ) T cells . ^^^ Both unfractionated CD 4 ( + ) and purified CD 25 ( + ) Treg cells fully protected the recipients against the development of colitis . ^^^ The present data demonstrate DC induced CD 4 ( + ) CD 25 ( + ) Treg cells , which phenotypically and functionally differ from the generally accepted prototype of CD 25 ( + ) Treg cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Data regarding the role of TGF beta for the in vivo function of regulatory CD 4 ( + ) CD 25 ( + ) T cells ( Treg ) are controversial . ^^^ A transgenic mouse model with impaired TGF beta signaling specifically in T cells was used to assess the role of endogenous TGF beta for the in vivo function of CD 4 ( + ) CD 25 ( + ) Treg in a murine model of colitis induced by dextran sulfate . ^^^ Transfer of wild type , but not transgenic CD 4 ( + ) CD 25 ( + ) Treg was found to suppress colitis in wild type mice . ^^^ In addition , by transferring CFSE labeled CD 4 ( + ) CD 25 ( + ) Treg we could demonstrate that endogenous TGF beta promotes the expansion of CD 4 ( + ) CD 25 ( + ) Treg in vivo . ^^^ Transgenic mice themselves developed reduced numbers of peripheral CD 4 ( + ) CD 25 ( + ) Treg and were more susceptible to the induction of colitis , which could be prevented by the transfer of wild type Treg . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) T cells with a memory phenotype ( CD45R0 ( + ) ) expressing CD 25 and CD 26 were the predominant cell type responding to antigens . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Finally , the potential of this technology to analyze rare primary cell populations is shown in a study of differential STAT protein phosphorylation in interleukin ( IL ) 2 stimulated CD 4 ( + ) CD 25 ( + ) regulatory T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Indirect immunofluorescence was used to determine the count of CD 3 , CD 4 , CD 80 , CD 38 , CD 25 , CD 71 , and CD 95 lymphocytes . ^^^ Unlike cancer diseases , pulmonary tuberculosis was characterized by the combination of a significantly inverse correlation between the relative count of CD 3 , CD 4 , CD 8 lymphocytes the levels of thymogen antibodies with a significantly direct correlation between the absolute count of CD 25 and CD 38 lymphocytes . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Moreover , CD 4 ( + ) T cell lines directed against HC gp 39 expressed CD 25 , glucocorticoid induced tumor necrosis factor receptor , and Foxp 3 molecules and were capable of suppressing antigen specific T cell responses . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Typical cell surface antigens involved in wound healing were studied [ cluster of differentiation ( CD ) 2 , CD 3 , CD 4 , CD 25 and human leukocyte antigen ( HLA ) diabetic retinopathy ( DR ) ] . ^^^ RESULTS : known to adversely affect wound healing were elevated in diabetic patients ( CD 2 p < 0 . 001 ; CD 3 p=0 . 016 , CD 4 p=0 . 22 , CD 25 p < 0 . 001 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The frequency of CD 25 + memory CD 4 + T cells and transient FOXP 3 mRNA expression by CD 4 + T cells significantly increased after systemic glucocorticoid treatment , whereas TGFB 1 expression did not change . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 25 ( + ) CD 4 ( + ) regulatory T cells ( Tregs ) are required for the maintenance of peripheral tolerance to certain self Ags . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Naturally occurring CD 4 ( + ) regulatory T cells are generally identified through their expression of CD 25 . ^^^ However , in several experimental systems considerable T ( reg ) activity has been observed in the CD 4 ( + ) CD 25 ( ) fraction . ^^^ Upon adoptive transfer , the expression of CD 25 in donor derived cells is not stable , with CD 4 ( + ) CD 25 ( + ) cells appearing in CD 4 ( + ) CD 25 ( ) T cell injected animals and vice versa . ^^^ The maintenance of CD 25 expression by CD 4 ( + ) CD 25 ( + ) cells depends on IL 2 secreted by cotransferred CD 4 ( + ) CD 25 ( ) or by Ag stimulated T cells in peripheral lymphoid organs . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Autoimmunity presumably manifests as a consequence of a shortfall in the maintenance of peripheral tolerance by CD 4 ( + ) CD 25 ( + ) T regulatory cells ( Tregs ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Tolerized ILT 3 ( high ) , ILT 4 ( high ) APC anergize CD 4 ( + ) T ( H ) cells and can induce the generation of antigen specific CD 4 ( + ) CD 25 ( + ) T regulatory cells ( T ( R ) ) cells and CD 8 ( + ) CD 28 ( ) T ( S ) cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
T cell subgroups , dendritic cell ( DC ) subsets , CD 4 ( + ) CD 25 ( + ) regulatory T cells and the expression of CD28 / CD80 / CD86 molecules on monocytes , B and T cells were analyzed using flow cytometry . ^^^ The number of monocytes per microliter was increased 2 . 13 fold , while the numbers of CD 4 ( + ) CD 25 ( + ) regulatory T cells were unchanged . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) CD 69 ( + ) cells were also increased in long term surviving patients . ^^^ The decrease in the percentage of CD 28 ( + ) cells and the increase in CD 4 ( + ) CD 25 ( + ) CD 69 ( + ) cells suggest that regulatory mechanisms of the immune response are still active in such patients . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The number of positive cell for CD 3 , CD 4 , CD 8 , CD 25 , CD 28 , CTLA 4 ( CD 152 ) , Fas , FasL , bcl 2 , bcl 10 were determined using immunohistochemical staining techniques . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
METHODS : Antigen specific T and B cell activation was studied in immunized Balb / c mice against 57 kDa antigen by proliferative responses using [ 3H ] thymidine incorporation and avidin biotin complex ( ABC ) peroxidase staining for CD 4 , CD 8 , CD 3 , CD 22 , and CD 25 followed by IL 2 and IL 4 estimation . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In addition , treatment with anti CD 25 Ab to deplete CD 4 ( + ) CD 25 ( + ) regulatory T cells before DC vaccination considerably improved the effect of the vaccine and allowed the development of long lived immune responses that were tumor protective . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Compared with normal B 6 mice , IFNAR ( / ) or IFN beta ( / ) mice have normal numbers of CD 4 ( + ) and CD 8 ( + ) T cells , and CD 25 ( + ) FoxP 3 ( + ) T regulatory ( T ( R ) ) cells in liver and spleen . ^^^ CD 8 ( + ) T cell responses are thus subjected to negative control by both CD 25 ( + ) FoxP 3 ( + ) T ( R ) cells and CD 4 ( + ) IL 10 ( + ) T ( R 1 ) cells , but only development of the latter T ( R ) cells depends on type 1 IFN . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Prior reports have shown that CD 4 ( + ) CD 25 ( + ) regulatory T cells suppress naive T cell responses by inhibiting IL 2 production . ^^^ In this report , using an Ag specific TCR transgenic system , we show that naive T cells stimulated with cognate Ag in the presence of preactivated CD 4 ( + ) CD 25 ( + ) T cells also become refractory to the mitogenic effects of IL 2 . ^^^ This IL 2 unresponsiveness did not require the continuous presence of CD 4 ( + ) CD 25 ( + ) regulatory T cells . ^^^ The mitotic block was also dissociated from the antiapoptotic effects of IL 2 , because IL 2 still promoted the survival of T cells that had been cocultured with CD 4 ( + ) CD 25 ( + ) T cells . ^^^ Our results therefore show that T cell activation in the presence of CD 4 ( + ) CD 25 ( + ) regulatory T cells can induce an alternative stimulation program characterized by up regulation of high affinity IL 2R , but a failure to produce IL 2 , and uncoupling of the mitogenic and antiapoptotic effects of IL 2 . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We found that a CD 4 ( + ) T lymphocyte subpopulation in peripheral blood , phenotypically CD 25 ( high ) , CTLA 4 ( + ) , Foxp 3 ( high ) ( regulatory T ( Treg ) cells ) , is deficient in its suppressor activity in psoriasis . ^^^ Thus , dysfunctional blood and target tissue CD 4 ( + ) CD 25 ( high ) Treg cell activity may lead to reduced restraint and consequent hyperproliferation of psoriatic pathogenic T cells in vivo . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Treatment with granulocyte colony stimulating factor prevents diabetes in NOD mice by recruiting plasmacytoid dendritic cells and functional CD 4 ( + ) CD 25 ( + ) regulatory T cells . ^^^ G CSF recipients further displayed accumulation of functional CD 4 ( + ) CD 25 ( + ) regulatory T cells that produce transforming growth factor beta 1 ( TGF beta 1 ) and actively suppressed diabetes transfer by diabetogenic effector cells in secondary NOD SCID recipients . ^^^ G CSF ' s ability to promote key tolerogenic interactions between DCs and regulatory T cells was demonstrated by enhanced recruitment of TGF beta 1 expressing CD 4 ( + ) CD 25 ( + ) cells after adoptive transfer of DCs isolated from G CSF relative to vehicle treated mice into naive NOD recipients . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Defective suppressor function in CD 4 ( + ) CD 25 ( + ) T cells from patients with type 1 diabetes . ^^^ The mechanisms involved in maintenance of peripheral tolerance include a specialized subset of regulatory T cells ( Treg ) within the CD 4 ( + ) CD 25 ( + ) T cell population , but the function and phenotype of these cells in type 1 diabetes have not been investigated . ^^^ We hypothesized that a deficiency in the CD 4 ( + ) CD 25 ( + ) Treg population or its function could contribute to the lack of self tolerance evident in patients with type 1 diabetes . ^^^ We show that although levels of CD 4 ( + ) CD 25 ( + ) T cells are normal in patients with recent onset adult type 1 diabetes , the ability of the Tregs in this population to suppress T cell proliferation during in vitro cocultures is markedly reduced compared with control subjects ( P = 0 . 007 ) . ^^^ These deficiencies may reflect a disturbance in the balance of the CD 4 ( + ) CD 25 ( + ) population , because in patients with type 1 diabetes , a higher proportion of these cells coexpress the early activation marker CD 69 ( P = 0 . 007 ) and intracellular CTLA 4 ( P = 0 . 01 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Here we show that H . pylori infected individuals have increased frequencies of CD 4 ( + ) CD 25 ( high ) T cells in both the stomach and duodenal mucosa compared to uninfected controls . ^^^ In contrast , mucosal CD 4 ( + ) CD 25 ( low ) and CD 4 ( + ) CD 25 ( ) cells express little FOXP 3 mRNA and low levels of the CTLA 4 protein . ^^^ Mucosal CD 4 ( + ) CD 25 ( high ) T cells are present in individuals with asymptomatic H . pylori infections as well as in duodenal ulcer patients . ^^^ The frequencies of CD 4 ( + ) CD 25 ( high ) cells are also increased in the stomachs of H . pylori infected patients with gastric adenocarcinoma , particularly in cancer affected tissues . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In order to determine whether FOXP 3 expression is confined to CD 4 ( + ) CD 25 ( + ) T cells with a regulatory phenotype , we analyzed several well defined T cell clones and lines with various specificities . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Here , we investigated the interference of Ag specific CD 4 ( + ) CD 25 ( + ) regulatory T cells ( Treg ) with the tumor specific CD 8 T cell immune response in vivo , by monitoring the homing , expansion , and effector function of both subsets in draining and nondraining lymph nodes . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We also examined other T cell receptors , such as CD 4 , CD 25 , and CD 69 , but they did not change for up to 24 h following exposure . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
An age dependent differential expression of B 7 1 and B 7 2 was associated with the development of insulitis and CD 4 ( + ) CD 25 ( + ) T cell deficiency in autoimmune disease prone mice . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Incubation of purified C57BL / 6 murine CD 4 ( + ) T lymphocytes with anti CD 3 mAb serves as a model of TCR mediated activation and results in increased IFN gamma production and cell surface expression of CD 25 and CD 69 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Adoptive transfer of purified splenic population of CD 4 ( + ) CD 25 ( + ) but not CD 4 ( + ) CD 25 ( ) cells from immunized mice into naive recipients prevented the induction of autoantibody production . ^^^ Altogether , our data establish a critical role for CD 4 ( + ) CD 25 ( + ) cells for control of AIHA , which may help to establish therapeutic strategies for treatment of AIHA . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In particular , even difficult to detect antigens such as CD 4 , cyclin D 1 , granzyme beta , bcl 6 , CD 25 , and lambda chain revealed distinct immunostainings . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
At 18 26 months EBNA 1 transgenic mice did not differ from littermate control in ultimate body weight , in spleen size or weight , in lymph node , kidney , liver , or spleen histology , in splenocyte fractions positive for cluster of differentiation ( CD ) 3epsilon , CD 4 , CD 8 , CD62L , B 220 , CD 5 , IgM , IgD , MHC class 2 , CD11b , or CD 25 , or in serum IgM , IgG , or total Ig levels . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
KIL depends on interleukin 7 for survival and proliferation and is NK1 . 1 ( + ) CD 3 ( ) TCRalphabeta ( ) TCRdeltagamma ( ) CD 4 ( ) CD 8 ( ) CD 19 ( ) CD 25 ( + ) CD 43 ( + ) CD 45 ( + ) CD49b ( ) CD 51 ( + ) CD 94 ( + ) NKG2D ( + ) Mac 1 ( / low ) B 220 ( ) c kit ( + ) perforin 1 ( + ) granzyme B ( + ) Notch 1 ( + ) , and cytotoxic . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
This revealed an association between peripheral blood cell numbers of specific lymphocyte subpopulations ( CD 4 ( + ) , CD 3 ( + ) CD 25 ( + ) , and nave T cells ) and serum levels of markers for AITD ( free thyroxine [ T ( 4 ) ] and thyroid stimulating immunoglobulin ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Foxp 3 , which encodes the transcription factor scurfin , is indispensable for the development and function of CD 4 ( + ) CD 25 ( + ) regulatory T cells ( Treg ) . ^^^ Recent data suggest conversion of peripheral CD 4 ( + ) CD 25 ( ) nai ve T cells to CD 4 ( + ) CD 25 ( + ) Treg by acquisition of Foxp 3 through costimulation with TCR and TGF beta or forced expression of the gene . ^^^ Moreover , we confirm the expression of both Foxp 3 and HO 1 in peripheral CD 4 ( + ) CD 25 ( + ) Treg and suppressive function of the cells are relieved by the inhibition of HO 1 activity . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The population of CD 4 ( + ) CD 25 ( + ) T cells from DKO mice lacks regulatory capacity , except a small subpopulation that highly expresses glucocorticoid induced tumor necrosis factor receptor family related gene ( GITR ) and CD 25 . ^^^ However , neither wild type nor DKO CD 4 ( + ) CD 25 ( + ) regulatory T cells ( T reg cells ) are able to suppress proliferation of DKO CD 4 ( + ) CD 25 ( ) T helper cells . ^^^ Therefore , combined NFATc2 / c3 deficiency is compatible with the development of CD 4 ( + ) CD 25 ( + ) T reg cells but renders conventional CD 4 ( + ) T cells unresponsive to suppression , underlining the importance of NFAT proteins for sustaining T cell homeostasis . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
RESULTS : When compared with healthy pregnant women , both GD cohorts showed higher percentages CD 4 ( + ) CD 25 ( + ) ( P < 0 . 05 ) , CD 4 ( + ) CD45RO ( + ) ( P < 0 . 05 ) and CD 4 ( + ) CD 29 ( + ) ( P < 0 . 01 ) but lower percentages of CD 4 ( + ) CD45RA ( + ) ( P < 0 . 05 ) . ^^^ Higher percentages of the activated phenotypes CD 8 ( + ) CD 25 ( + ) and CD 8 ( + ) HLA DR ( + ) cells in the diet treated cohort and CD 4 ( + ) HLA DR ( + ) cells in insulin treated GB cohort , were observed compared with healthy pregnant subjects ( P < 0 . 05 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
New data show that the sialidase from Clostridium perfringens , but not from Vibrio cholerae , can increase activation of CD 4 cells from both old and young mice as measured by calcium signals , expression of CD 25 and CD 69 , and secretion of IL 2 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The activity of these DCs also failed to sustain robust T cell proliferation and , rather , functioned to enhance the suppressive efficacy of CD 4 ( + ) CD 25 ( + ) T regulatory cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Regulatory T ( Tr ) cell activity is enriched within the naturally arising CD 4 ( + ) CD 25 ( + ) Tr subset that has been shown to prevent a number of inflammatory diseases . ^^^ CD 4 ( + ) CD 25 ( + ) Tr cells control intestinal inflammation induced by both innate and adaptive immune responses via IL 10 and TGF beta dependent mechanisms . ^^^ Recent results have shown that CD 4 ( + ) CD 25 ( + ) Tr cells can cure established colitis , suggesting their utility for the treatment of inflammatory bowel disease . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Peyer ' s patch dendritic cells capturing oral antigen interact with antigen specific T cells and induce gut homing CD 4 ( + ) CD 25 ( + ) regulatory T cells in Peyer ' s patches . ^^^ Some of these T cells acquired a similar phenotype to CD 4 ( + ) CD 25 ( + ) regulatory T cells and CCR 9 ( + ) gut homing T cells . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Recent evidence suggests a deficit in suppressive activity of both CD 4 ( + ) CD 25 ( + ) regulatory T cells , and interleukin ( IL ) 10 producing regulatory T cells may play a role in development of allergic sensitization . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Mice lacking an essential component of the pre TCR exhibit arrest at the ( CD 4 ( ) CD 8 ( ) ) ( CD 44 ( ) CD 25 ( + ) ) stage ( DN 3 ) of thymocyte development , and introduction of p 53 deficiency into those mice abrogates this arrest , resulting in transition to the ( CD 4 ( + ) CD 8 ( + ) ) double positive ( DP ) stage . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Tolerance is dependent on the presence and regulatory function of CD 4 ( + ) CD 25 ( + ) T cells in a number of animal models . ^^^ The direct effects of immunosuppressive drugs on CD 4 ( + ) CD 25 ( + ) cells , particularly those that interfere with IL 2 signaling are uncertain . ^^^ We studied the effects of the rapamycin derivative everolimus and the anti CD 25 monoclonal antibody basiliximab on the regulatory capacity of human CD 4 ( + ) CD 25 ( + ) cells in vitro . ^^^ Both drugs permitted the suppression of proliferation and IFN gamma secretion by CD 4 ( + ) CD 25 ( ) cells responding to allogeneic and other polyclonal stimuli ; CTLA 4 expression was abolished on CD 4 ( + ) CD 25 ( + ) cells without compromising their suppressive ability . ^^^ Everolimus reduced IFN gamma secretion by CD 4 ( + ) CD 25 ( ) cells before the anti proliferative effect : this is a novel finding . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
PRL induces survival , proliferation and differentiation of lymphoid progenitors whereas both an anti PRL antiserum and an anti PRL R mAb block T cell development accumulating CD 25 ( + ) DN ( CD 4 ( ) CD 8 ( ) ) cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The importance of CD 25 ( + ) CD 4 ( + ) regulatory T ( Treg ) cells in the control of immune responses is established , but their antigen specificity in vivo remains unclear . ^^^ Pretreatment of recipient mice with donor alloantigen plus anti CD 4 antibody generates CD 25 ( + ) CD 4 ( + ) Treg cells with the capacity to prevent skin allograft rejection in adoptive transfer recipients . ^^^ We demonstrate that bystander regulation also extends to CD 25 ( + ) CD 4 ( + ) Treg cells generated in vivo by exposure to nominal antigens under anti CD 4 antibody cover . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In recent years a new category of the CD 4 ( + ) CD 25 ( + ) T regulatory ( Treg ) lymphocyte has been identified that maintains immune tolerance to self , and also functions in the regulation of parasite induced immunopathology . ^^^ The Foxp 3 gene which encodes the transcription factor Scurfin was found to be expressed by and required for the generation of CD 4 ( + ) CD 25 ( + ) T reg . ^^^ The percentage of granuloma CD 4 ( + ) CD 25 ( + ) T cells rose from 12 % at 8 weeks to 88 % at 16 weeks of the infection . ^^^ Foxp 3 expression was 3 . 5 fold higher in the CD 4 ( + ) CD 25 ( + ) versus the CD 4 ( + ) CD 25 ( ) T cells in the 8 week infection granulomas . ^^^ As a novel observation neuropilin 1 membrane expression , a recently identified marker for Treg , was correlated with Foxp 3 expression in the granuloma CD 4 ( + ) CD 25 ( + ) but not the CD 25 ( ) cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 25 ( + ) CD 4 ( + ) regulatory T cells exert in vitro suppressive activity independent of CTLA 4 . ^^^ Cytotoxic T lymphocyte antigen 4 ( CTLA 4 ) is constitutively expressed on CD 25 ( + ) CD 4 ( + ) regulatory T cells ( Treg ) and is suggested to play a role in Treg mediated suppression . ^^^ Furthermore , CD 25 ( + ) CD 4 thymocytes from young CTLA 4 deficient mice were analyzed and found to exhibit suppressive activity . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
However , adoptive transfer of CD 4 ( + ) CD 25 ( ) Th cells ( Th cells ) with tumor / self reactive CD 8 ( + ) T cells and vaccination into CD 4 ( + ) T cell deficient hosts induced autoimmunity and regression of established melanoma . ^^^ Transfer of CD 4 ( + ) T cells that contained a mixture of Th and CD 4 ( + ) CD 25 ( + ) T regulatory cells ( T ( reg ) cells ) or T ( reg ) cells alone prevented effective adoptive immunotherapy . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Active suppression mediated by CD 4 ( + ) CD 25 ( + ) T regulatory ( Tr ) cells plays an important role in the down regulation of T cell responses to both foreign and self Ags . ^^^ In this report we show that human PF 4 stimulates proliferation of the naturally anergic human CD 4 ( + ) CD 25 ( + ) Tr cells while inhibiting proliferation of CD 4 ( + ) CD 25 ( ) T cells . ^^^ In coculture experiments we found that CD 4 ( + ) CD 25 ( + ) Tr cells exposed to PF 4 lose the ability to inhibit the proliferative response of CD 4 ( + ) CD 25 ( ) T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Additional separation of CD 4 ( + ) T cells into CD 4 ( + ) CD 25 ( + ) and CD 4 ( + ) CD 25 ( ) subpopulations before their adoptive transfer into Rag 1 ( / ) mice showed that CD 4 ( + ) CD 25 ( + ) T cells were capable of reducing TLR stimulated cytokine production levels to WT levels , whereas CD 4 ( + ) CD 25 ( ) T cells had no regulatory effect . ^^^ These findings suggest a previously unsuspected role for CD 4 ( + ) CD 25 ( + ) T regulatory cells in controlling host inflammatory responses after injury . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We have recently reported that CD 4 ( + ) CD 25 ( + ) regulatory T cells mediate peripheral tolerance induced with mouse Tg in CBA mice . ^^^ To determine whether these cells play a role in our E ( + ) transgenic model , we first confirmed the existence of CD 4 ( + ) CD 25 ( + ) T cells regulating thyroiditis in E ( + ) B10 . ^^^ The depletion of CD 4 ( + ) CD 25 ( + ) T cells enhanced thyroiditis induction in the context of either H2E or H2A . ^^^ Moreover , reconstitution of CD 4 ( + ) CD 25 ( + ) T cells from naive B 10 mice restored resistance to EAT . ^^^ E ( + ) B 10 ( A ( + ) E ( + ) ) mice were also depleted of CD 4 ( + ) CD 25 ( + ) T cells before the challenge to determine their role in thyroiditis in the presence of both H2A and H2E genes . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In all uraemic patients , irrespective of the stage of renal insufficiency or dialysis modality , a significant increase in the percentage of CD 25 positivity in naive CD 4 ( + ) T cells was found ( 64 % + / 21 % versus 23 % + / 18 % , P < 0 . 001 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Little is known about the ontogeny of naturally occurring CD 4 ( + ) CD 25 ( + ) regulatory / suppressor T cells that play a major role in maintaining self tolerance in mice and humans . ^^^ Together with the first mature T cells , thymic CD 4 ( + ) CD 25 ( + ) cells were detected as early as 13 weeks of gestation . ^^^ Thymic CD 25 ( + ) cells appeared to be positively selected at the CD 4 ( + ) CD 8 ( + ) CD 3 ( hi ) differentiation stage , as assessed by CD1a and CD 69 expression . ^^^ The proportion of thymic CD 4 ( + ) CD 25 ( + ) cells appeared quite stable with age , around 6 % to 7 % , similar to the proportion observed in infant thymi . ^^^ Extrathymic CD 4 ( + ) CD 25 ( + ) T cells could hardly be detected at 13 weeks of gestation but were present from week 14 onwards . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) Treg cells do not secrete interleukin ( IL ) 10 and transforming growth factor beta cytokines but express CD 25 , the glucocorticoid induced tumor necrosis factor receptor related protein ( GITR ) , and Forkhead Box P 3 ( Foxp 3 ) , and are capable of suppressing the proliferative responses of naive CD 4 ( + ) and CD 8 ( + ) T cells to stimulation with mitogenic anti CD 3 antibody . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The percentage CD 4 ( + ) CD 25 ( + ) cells appeared lower with regimen 2 : 21 % ( 10 88 ) vs . regimen 1 : 50 % ( 0 87 ) ; p = 0 . 04 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Treg activity was characterized by quantifying CD 4 ( + ) CD 25 ( + ) cells , foxp 3 expression , and interleukin 10 secretion . ^^^ Compared to virgin CBA / J females , normal pregnant mice showed strongly elevated numbers of CD 4 ( + ) CD 25 ( + ) and interleukin 10 ( + ) Treg cells in the thymus whereas significantly lower frequencies of Treg cells were observed in abortion mice . ^^^ Very interestingly , CD 4 ( + ) CD 25 ( + ) Treg cells from normal pregnant and nonpregnant CBA / J mice could inhibit both proliferation and interferon gamma secretion of lymphocytes from abortion mice in vitro whereas in vivo prevention of fetal rejection could only be achieved after adoptive transfer of Treg cells from normal pregnant mice . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Here , we provide new evidence that rapamycin selectively expands the murine naturally occurring CD 4 ( + ) CD 25 ( + ) FoxP 3 ( + ) Tr cells in vitro . ^^^ Based on this new mode of action , rapamycin can be used to expand CD 4 ( + ) CD 25 ( + ) FoxP 3 ( + ) Tr cells for ex vivo cellular therapy in T cell mediated diseases . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Definition of target antigens for naturally occurring CD 4 ( + ) CD 25 ( + ) regulatory T cells . ^^^ The antigenic targets recognized by naturally occurring CD 4 ( + ) CD 25 ( + ) regulatory T cells ( T reg cells ) have been elusive . ^^^ CD 4 ( + ) CD 25 ( + ) T cells from mice immunized with SEREX defined self antigens had strong suppressive activity on peptide specific proliferation of CD 4 ( + ) CD 25 ( ) T cells and CD 8 ( + ) T cells . ^^^ Foxp 3 expression in these CD 4 ( + ) CD 25 ( + ) T cells from immunized mice was 5 10 times greater than CD 4 ( + ) CD 25 ( + ) T cells derived from naive mice . ^^^ We propose that SEREX defined self antigens such as those used in this study represent self antigens that elicit naturally occurring CD 4 ( + ) CD 25 ( + ) T reg cells . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Homeostatic maintenance of natural Foxp 3 ( + ) CD 25 ( + ) CD 4 ( + ) regulatory T cells by interleukin ( IL ) 2 and induction of autoimmune disease by IL 2 neutralization . ^^^ Such treatment selectively reduces the number of Foxp 3 expressing CD 25 ( + ) CD 4 ( + ) T cells , but not CD 25 ( ) CD 4 ( + ) T cells , in the thymus and periphery of normal and thymectomized mice . ^^^ IL 2 neutralization inhibits physiological proliferation of peripheral CD 25 ( + ) CD 4 ( + ) T cells that are presumably responding to normal self antigens , whereas it is unable to inhibit their lymphopenia induced homeostatic expansion in a T cell deficient environment . ^^^ In normal naive mice , CD 25 ( low ) CD 4 ( + ) nonregulatory T cells actively transcribe the IL 2 gene and secrete IL 2 protein in the physiological state . ^^^ IL 2 is thus indispensable for the peripheral maintenance of natural CD 25 ( + ) CD 4 ( + ) regulatory T cells ( T reg cells ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
T cells that can not respond to TGF beta escape control by CD 4 ( + ) CD 25 ( + ) regulatory T cells . ^^^ CD 4 ( + ) CD 25 ( + ) regulatory T ( T reg ) cells play a pivotal role in control of the immune response . ^^^ CD 4 ( + ) CD 25 ( + ) T reg cells from the thymus of dnTbetaRII mice retain the ability to inhibit colitis , suggesting that T cell responsiveness to TGF beta is not required for the development or peripheral function of thymic derived T reg cells . ^^^ In contrast , T reg cell activity among the peripheral dnTbetaRII CD 4 ( + ) CD 25 ( + ) population is masked by the presence of colitogenic effector cells that can not be suppressed . ^^^ Finally , we show that CD 4 ( + ) CD 25 ( + ) T reg cells develop normally in the absence of TGF beta 1 and retain the ability to suppress colitis in vivo . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Essential role for interleukin 2 for CD 4 ( + ) CD 25 ( + ) T regulatory cell development during the neonatal period . ^^^ Although many aspects of CD 4 ( + ) CD 25 ( + ) T regulatory ( T ( reg ) ) cell development remain largely unknown , signaling through the IL 2R represents one feature for the production of T ( reg ) cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We have observed a selective accumulation of CD 4 ( + ) CD 25 ( + ) T cells inside tumors . ^^^ We show here that CD 4 ( + ) CD 25 ( + ) T cells suppressed the proliferation and interferon gamma production of CD 8 ( + ) T cells in vivo at the local tumor site . ^^^ Therefore , CD 4 ( + ) CD 25 ( + ) T cells maintained an environment in the tumor that concealed the immunogenicity of tumor cells to permit progressive growth of antigenic tumors . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Foxp3+ CD 25 CD 4 T cells constitute a reservoir of committed regulatory cells that regain CD 25 expression upon homeostatic expansion . ^^^ Expression of the IL 2 receptor alpha chain ( CD 25 ) by peripheral CD 4 T cells follows cellular activation . ^^^ However , CD 25 expression by CD 4 cells is widely used as a marker to identify regulatory T cells ( T ( R ) ) , although cells with regulatory properties are also found in the CD4+CD25 subset . ^^^ Surface expression of CD 25 on T ( R ) is controlled by the activity of conventional CD 4 cells and is fully labile because it can be lost and regained without affecting the functional potential of the cells . ^^^ This analysis , together with the notion that physiological commitment of T ( R ) takes place exclusively in the thymus should help for the interpretation of experiments assessing peripheral T ( R ) differentiation from naive CD 4 T cells , defined as CD 25 . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) regulatory T cells are thought to be generated in the periphery as well as in the thymus . ^^^ We sought to determine the roles played by CD 4 ( + ) CD 25 ( + ) T cells and transforming growth factor beta ( TGF beta ) in the induction and maintenance of tolerance generated by oral antigens in BALB / c mice . ^^^ Depletion of CD 4 ( + ) CD 25 ( + ) cells after mice were orally tolerized did not reverse the tolerant status , indicating that these cells were not required to maintain the established tolerance . ^^^ CD 25 depletion together with TGF beta neutralization led the expansion of OVA specific CD 4 T cells against the subsequent antigen challenge , and each treatment alone did not . ^^^ Our findings indicate that CD 4 ( + ) CD 25 ( + ) T cells and TGF beta play a complementary role in the induction of oral tolerance , at least in part , by regulating the expansion of antigen specific CD 4 T cells . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
A very early ( 24 hours after infection ) and strong induction of TGF beta 1 and FoxP 3 expression was detected and correlated with increases in levels of CD 4 ( + ) CD 25 ( + ) and CD 8 ( + ) CD 25 ( + ) T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Although in animal experiments the role of CD 4 ( + ) CD 25 ( + ) T regulatory cells ( T ( reg ) ) in protection against autoimmunity is well established , the role of these cells in AAV is unknown . ^^^ To investigate the hypothesis that an increased expression of CD 25 on T cells is related to persistent T cell activation and not to disturbances in T ( reg ) cells in AAV ( 34 patients , six of them after renal transplantation ) , we investigated CD 25 expression in different subpopulations of CD 4 ( + ) cells and FOXP 3 mRNA expression by reverse transcription polymerase chain reaction ( RT PCR ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) and CD1d restricted natural killer T ( NKT ) cells are thymus derived self reactive regulatory T cells that play a key role in the control of pathological immune responses . ^^^ Little is known about functional cooperation between innate regulatory NKT cells and adaptive CD 4 ( + ) CD 25 ( + ) regulatory cells . ^^^ When cocultured with CD 4 ( + ) CD 25 ( + ) cells , CD 4 ( + ) NKT cells promoted moderate proliferation of CD 4 ( + ) CD 25 ( + ) cells . ^^^ The proliferation of CD 4 ( + ) CD 25 ( + ) T cells was due to soluble IL 2 produced by activated CD 4 ( + ) NKT cells . ^^^ The expanded CD 4 ( + ) CD 25 ( + ) cells remained anergic and retained their potent suppressive properties . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Both naturally occurring CD 4 ( + ) CD 25 ( + ) regulatory T cells and inducible populations of antigen specific interleukin 10 secreting regulatory T cells inhibit these inappropriate immune responses in experimental models . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) T cells have immunoregulatory and suppressive functions and are responsible for suppressing self reactive cells and maintaining self tolerance . ^^^ In addition to CD 4 ( + ) CD 25 ( + ) T cells , there is some evidence that a fraction of CD 4 ( + ) CD 25 ( ) T cells exhibit suppressive activity in vitro or in vivo . ^^^ We have shown , using aged mice , that aging not only leads to a decline in the ability to mount CD 4 ( + ) CD 25 ( ) T cell responses , but , at the same time , renders aged CD 4 ( + ) CD 25 ( ) T cells suppressive . ^^^ In this study we report two newly established mAbs that could abrogate the suppressive function of aged CD 4 ( + ) CD 25 ( ) T cells . ^^^ Cross linking of CD 45 on aged CD 4 ( + ) CD 25 ( ) T cells was required for the disruption of their suppressive activity . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Regulatory T cells ( Tregs ) are CD 4 ( + ) CD 25 ( bright ) CD62L ( high ) cells that actively down regulate immune responses . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 25 ( + ) CD 4 ( + ) regulatory T cells suppress immune responses and are believed to play roles in preventing autoimmune diseases . ^^^ Here we show that these regulatory T cells downregulated CD 25 ( ) CD 4 ( + ) T cell mediated production of IL 12 from antigen presenting cells , which can act as a growth factor for CD 25 ( ) CD 4 ( + ) T cells . ^^^ We further found that CD 25 ( + ) CD 4 ( + ) T cells , despite their well documented `` anergic ' ' nature , proliferate significantly in vitro only when CD 25 ( ) CD 4 ( + ) T cells are present . ^^^ Thus , CD 25 ( + ) CD 4 ( + ) T cells suppress CD 25 ( ) CD 4 ( + ) T cell responses , at least in part , by inhibiting IL 12 production while they themselves can undergo proliferation with the mediation of CD 25 ( ) CD 4 ( + ) T cells in vitro . ^^^ These results offer a novel negative feedback system involving a tripartite interaction among CD 25 ( + ) CD 4 ( + ) and CD 25 ( ) CD 4 ( + ) T cells , and APCs that may contribute to the termination of immune responses . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Because naturally occurring CD 4 ( + ) CD 25 ( + ) regulatory T cells ( natural CD 25 ( + ) Treg cells ) have been shown to inhibit allograft rejection , we investigated their influence on the outcome of allografts in nonimmunosuppressed mouse recipients . ^^^ Finally , we investigated the impact of natural CD 25 ( + ) Treg cells on the production of T helper ( Th ) 1 and Th 2 cytokines in mixed lymphocyte cultures between C57BL / 6 CD 4 ( + ) CD 25 ( ) T cells as responders and bm 12 or ( C57BL / 6X bm 12 ) F 1 antigen presenting cells as stimulators . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Mechanisms of immunosuppression by CD 4 ( + ) CD 25 ( + ) suppressor T cells have been addressed using many in vitro and in vivo conditions . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
This review discusses the control exerted by natural CD 4 ( + ) CD 25 ( + ) regulatory T cells ( natural T ( reg ) cells ) during infectious processes . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The first strategies targeting the negative influence of CD 4 ( + ) CD 25 ( + ) T regulatory cells and the CTLA 4 signaling pathway are just entering clinical testing in combination with tumor vaccines . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
PURPOSE : Adult T cell leukemia / lymphoma ( ATL ) is a peripheral CD 4 ( + ) CD 25 ( + ) T cell malignancy caused by human T cell leukemia virus type 1 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
A higher percentage of T ( reg ) , defined as CD 4 , CD 25 , CD45RO , and cytotoxic T lymphocyte associated antigen 4 positive cells , was detected within the population of CD 4 ( + ) cells in peripheral blood of chronic HBV patients compared with healthy controls and individuals with a resolved HBV infection . ^^^ Reconstitution of these depleted PBMC with CD 4 ( + ) CD 25 ( + ) T ( reg ) resulted in a dose dependent reduction of both HBV specific proliferation and interferon gamma production . ^^^ We propose that local immune responses in patients with HCC are held in check by tumor infiltrating CD 4 ( + ) CD 25 ( + ) T regulatory lymphocytes ( T ( reg ) cells ) , which suppress the activity and proliferation of effector CD 4 ( + ) and CD 8 ( + ) T cells . ^^^ CD 4 ( + ) CD 25 ( + ) T ( reg ) cells comprised 8 . 7 % ( 1 . 4 13 . 8 ) of TILs and always exceeded the proportion in distant nontumor tissue ( 2 . 4 % [ 1 . 5 5 . 6 ] ; P = . 014 ) . ^^^ T ( reg ) cells isolated from HCC suppressed proliferation of autologous circulating CD 4 ( + ) CD 25 ( ) cells and perforin expression and proliferation of autologous CD 8 ( + ) T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) regulatory T cells are generated in response to both forms of self antigens , but the induction is much more efficient with the tissue antigen . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In these situations , colitis occurs as a result of the absence of a specialized population of regulatory cells , as transfer of CD 4 ( + ) CD 25 ( + ) T cells prevents disease . ^^^ Importantly , from a clinical perspective , CD 4 ( + ) CD 25 ( + ) T cells can also reverse an established colitis . ^^^ CD 4 ( + ) CD 25 ( + ) T cells proliferate both in the secondary lymphoid organs and at the site of inflammation , suggesting that regulation occurs both locally and systemically . ^^^ CD 4 ( + ) CD 25 ( + ) T cells are not only capable of regulating other T cells but are also capable of suppressing components of the innate immune system . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
A search for peripheral T cells that might exert dominant inhibitory effects on autoreactivity has implicated a subpopulation of CD 4 ( + ) CD 25 ( + ) T cells called regulatory T cells ( Tregs ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In contrast , two million CD 4 ( + ) CD 25 ( + ) cells ( expressing Foxp 3 ) prevented the accelerated diabetes when transferred both before and simultaneously with the diabetogenic T cells . ^^^ In addition , 2 million CD 4 ( + ) CD 25 ( + ) T cells prevented spontaneous diabetes , even when given to rats age 42 days , whereas 20 million CD 4 ( + ) CD 25 ( ) cells ( with low Foxp 3 expression ) were far less effective . ^^^ We thus demonstrated that CD 4 ( + ) CD 25 ( + ) cells exhibit powerful regulatory potential in rat diabetes . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Detailed analysis of the draining lumbar lymph nodes revealed an increased frequency and number of CD 4 ( + ) CD 25 ( + ) T cells and an enhanced production of inhibitory cytokines , which correlated with impaired T cell function . ^^^ Functional studies confirmed a role for CD 4 ( + ) CD 25 ( + ) regulatory T cells in suppressing T cell proliferation as well as regulating the growth of transplanted prostate tumor cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
After 7 administrations , OVA specific proliferation and cytokine production of mononuclear cells of the spleen , mesenteric lymph nodes , and Peyer ' s patches and the number of splenic CD 4 + CD 25 + T cells were analyzed . ^^^ The number of OVA specific TCR + T cells decreased in the spleen from OVA fed mice , whereas the number of CD 4 + CD 25 + T cells increased . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
There may also be a role for CD 4 ( + ) CD 25 ( + ) T ( reg ) , but how and when all these different mechanisms operate is still unclear . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) regulatory T ( T ( reg ) ) cells play a critical role in the maintenance of peripheral tolerance and the prevention of autoimmunity . ^^^ In the present study , we have explored the characteristics of CD 4 ( + ) CD 25 ( + ) T ( reg ) cells in patients with rheumatoid arthritis ( RA ) . ^^^ The frequency and phenotype of CD 4 ( + ) CD 25 ( + ) T cells in paired samples of synovial fluid ( SF ) and peripheral blood ( PB ) from patients with RA and PB from normal controls were analysed . ^^^ No significant difference was observed in the numbers of CD 4 ( + ) CD 25 ( + ) T cells in PB from patients and controls . ^^^ SF CD 4 ( + ) CD 25 ( + ) T cells expressed high levels of CTLA 4 ( both surface and intracellular ) , GITR and OX 40 , as well as Foxp 3 transcripts . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) regulatory T cells are selected in the thymus to control autoreactive thymic escapees preventing autoimmunity that can not be achieved by negative selection or deletion alone , thus playing an important role in the maintenance of immunological homeostasis . ^^^ Not only significant in preventing autoimmunity , CD 4 ( + ) CD 25 ( + ) regulatory T cells have also been shown to be involved in allograft tolerance in organ transplantation . ^^^ Using a heterotopic cardiac transplantation model , we show herein that intrathymic inoculation of donor HLA class 1 derived synthetic peptide results in the generation of CD 4 ( + ) CD 25 ( + ) regulatory T cells , which induce graft specific tolerance without any preconditioning of the recipient or use of immunosuppressive drugs . ^^^ This study provides evidence of the novel therapeutic potential of CD 4 ( + ) CD 25 ( + ) regulatory T cells for clinical transplantation . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In four kidney transplant patients with allograft rejection we observed a dramatic drop of circulating T and dendritic cells at the time of rejection , and while myeloid dendritic cells and CD 4 ( + ) and CD 8 ( + ) cells rapidly recovered after 1 month , plasmacytoid dendritic cells and CD 4 ( + ) CD 25 ( + ) T cell numbers remained significantly lower than in patients without rejection . ^^^ Future studies will evaluate the monitoring of circulating CD 4 ( + ) CD 25 ( + ) T cells and myeloid dendritic cell : plasmacytoid dendritic cell ratio as potential biomarkers for rejection or , alternatively , for withdrawal of immune suppression . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
OX 40 ( CD 134 ) , a member of the tumor necrosis factor receptor superfamily , is expressed on activated T cells , including CD 4 ( + ) CD 25 ( + ) T regulatory ( Treg ) cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Here , we report that TGF beta 1 supports the maintenance of Foxp 3 expression , regulatory function , and homeostasis in peripheral CD 4 ( + ) CD 25 ( + ) regulatory T ( T reg ) cells , but is not required for their thymic development . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Interestingly , while this protection correlated with an increase in the frequency of CD 4 ( + ) CD 25 ( + ) regulatory T cells , neither prior depletion of regulatory T cells nor anti TGF beta treatment abrogated the treatment ' s efficacy . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Both CD 25 ( + ) and CD 25 ( ) CD 4 ( + ) CD 8 ( + ) T cells are able to suppress adoptive transfer of Th 1 effector cells mediating cutaneous contact sensitivity . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Co administration of anti CD 40 mAb increased the synthesis of IL 2 and the expression of CD 25 by CD 8 T cells , but neither IL 2 production nor CD 25 expression by CD 4 T cells was enhanced by anti CD 40 mAb . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Although CD 4 ( + ) CD 25 ( + ) regulatory T cells ( Treg ) represent a well characterized population of T cells with in vitro and in vivo suppressive capacity , the basic mechanisms of suppression are still not understood . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Those antigen driven selections are seen not only in conventional CD 4 ( + ) and CD 8 ( + ) T cells but also in CD 4 ( + ) CD 25 ( + ) regulatory T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Natural CD 4 ( + ) CD 25 ( + ) regulatory T ( CD 4 ( + ) CD 25 ( + ) T reg ) cells play a key role in the immunoregulation of autoimmunity . ^^^ However , little is known about the interactions between CD 4 ( + ) CD 25 ( + ) T reg cells and autoreactive T cells . ^^^ This is due , in part , to the difficulty of using cell surface markers to identify CD 4 ( + ) CD 25 ( + ) T reg cells accurately . ^^^ Using a novel real time PCR assay , mRNA copy number of FoxP 3 , TGFbeta 1 , and interleukin ( IL ) 10 was measured in single cells to characterize and quantify CD 4 ( + ) CD 25 ( + ) T reg cells in the nonobese diabetic ( NOD ) mouse , a murine model for type 1 diabetes ( T1D ) . ^^^ The suppressor function of CD 4 ( + ) CD 25 ( + ) CD62L ( hi ) T cells , mediated by TGFbeta , declined in an age dependent manner . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Previously , we have shown that CD 4 ( + ) CD 25 ( + ) T cells by the virtue of their partially activated phenotype represent ideal candidates for a productive feline immunodeficiency virus ( FIV ) infection . ^^^ In the present study , we extended our previous observations with regard to FIV replication in CD 4 ( + ) CD 25 ( + ) and CD 4 ( + ) CD 25 ( ) cells under different stimulation conditions . ^^^ Both CD 4 ( + ) CD 25 ( + ) and CD 4 ( + ) CD 25 ( ) cells remain latently infected in the absence of IL 2 or concanvalinA ( ConA ) , respectively ; harboring a replication competent provirus capable of reactivation several days post infection . ^^^ While CD 4 ( + ) CD 25 ( + ) cells require low levels of exogenous IL 2 and virus inputs for an efficient FIV replication , CD 4 ( + ) CD 25 ( ) T cells can only be productively infected in the presence of either high concentrations of IL 2 or high virus titers , even in the absence of mitogenic stimulation . ^^^ Interestingly , while high virus input activates CD 4 ( + ) CD 25 ( ) cells to replicate FIV , it induces apoptosis in a high percentage of CD 4 ( + ) CD 25 ( + ) T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Over the past decade , a great deal of interest and attention has been directed toward a population of regulatory T cells ( Treg ) coexpressing the markers CD 4 and CD 25 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The growth of UV induced tumors appears to be controlled by UV induced suppressor T cells , because CD 80 ( / ) / CD86 ( / ) mice had strongly reduced numbers of UV induced CD 4 ( + ) CD 25 ( + ) suppressor T cells . ^^^ In vitro , CTLA 4 blockade inhibited the suppressor activity of UV induced CD 4 ( + ) CD 25 ( + ) T cells , suggesting that reduced photocarcinogenesis might be due to decreased numbers or function of suppressor T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Exposure to CT , but not to LT IIa or LT IIb , inhibited mitogen driven CD 4 ( + ) T cell proliferation and expression of CD 25 and CD 69 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Increasing evidence indicates that CD 4 ( + ) CD 25 ( + ) T regulatory ( Treg ) cells control a wide spectrum of immune responses . ^^^ The initial identification of CD 4 ( + ) CD 25 ( + ) Treg cell as a `` professional suppressor ' ' was based on observations made in BALB / c mice . ^^^ We observed that the frequency of CD 4 ( + ) CD 25 ( + ) T cells in the thymus and peripheral lymphoid organs of BALB / c mice was higher than in B 6 mice . ^^^ CD 4 ( + ) CD 25 ( + ) Treg cells from both mouse strains shared similar phenotypic properties , including expression of characteristic immunological markers and hyporesponsiveness to T cell receptor cross linking and in their capacity to suppress proliferation of BALB / c CD 4 ( + ) CD 25 ( ) T responder ( Tres ) cells . ^^^ However , CD 4 ( + ) CD 25 ( ) Tres cells from B 6 mice were notably less susceptible to suppression by CD 4 ( + ) CD 25 ( + ) Treg cells from either mouse strain . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Immunotherapy against a variety of malignancies , including pleural based malignancies , has shown promise in animal models and early human clinical trials , but successful efforts will need to address immunosuppressive factors of the tumor and host , particularly certain cytokines and CD 4 ( + ) CD 25 ( + ) regulatory T cells ( Treg ) . ^^^ Although a high prevalence of functionally suppressive CD 4 ( + ) CD 25 ( + ) T cells was found in carcinomatous pleural effusions , mesothelioma pleural effusions contained significantly fewer CD 4 ( + ) CD 25 ( + ) T cells . ^^^ However , there is clear patient to patient variability and occasional mesothelioma patients with high percentages of CD 4 ( + ) CD 25 ( + ) pleural effusion T cells and low percentages of CD 8 ( + ) CD 25 ( + ) pleural effusion T cells can be identified . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
HIV 1 virions activated CD 4 ( + ) and CD 8 ( + ) T cells to express CD 25 and HLA DR and preferentially induced apoptosis in CD 25 ( + ) HLA DR ( + ) T cells in a CXCR 4 dependent manner . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) Tregs are important in the maintenance of immunological self tolerance and in the prevention of autoimmune diseases . ^^^ As the CD 4 ( + ) CD 25 ( + ) T cell population in patients with human T cell lymphotropic virus type 1 associated ( HTLV 1 associated ) myelopathy / tropical spastic paraparesis ( HAM / TSP ) has been shown to be a major reservoir for this virus , it was of interest to determine whether the frequency and function of CD 4 ( + ) CD 25 ( + ) Tregs in HAM / TSP patients might be affected . ^^^ In these cells , both mRNA and protein expression of the forkhead transcription factor Foxp 3 , a specific marker of Tregs , were lower than those in CD 4 ( + ) CD 25 ( + ) T cells from healthy individuals . ^^^ The virus encoded transactivating HTLV 1 tax gene was demonstrated to have a direct inhibitory effect on Foxp 3 expression and function of CD 4 ( + ) CD 25 ( + ) T cells . ^^^ These results suggest that direct human retroviral infection of CD 4 ( + ) CD 25 ( + ) T cells may be associated with the pathogenesis of HTLV 1 associated neurologic disease . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Almost a quarter of a century ago , Oldstone and colleagues proposed that infection of cells by noncytopathic viruses may lead to an alteration of the cells ' ability to produce certain products or perform certain tasks , i . e . , inhibition of `` luxury function . ' ' In this issue of the JCI , this topic has been revisited by Yamano et al . , who demonstrate that human T cell lymphotropic virus type 1 ( HTLV 1 ) infection of CD 4 ( + ) CD 25 ( + ) Tregs in patients with HTLV 1 associated myelopathy / tropical spastic paraparesis ( HAM / TSP ) results in a decrease in FOXP 3 mRNA and protein expression . ^^^ This leads to the inability of HTLV 1 infected CD 4 ( + ) CD 25 ( + ) Tregs to inhibit the proliferation of CD 4 ( + ) CD 25 ( ) Tregs , due to the effect of the HTLV 1 tax gene . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The sections were examined for the interleukins IL 4 , IL 6 , IL 8 , IL 10 and IFN gamma as well as for the cell markers CD 4 , CD 8 , CD 14 , Cd 19 , CD 25 and CD 30 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Naturally occurring CD 4 ( + ) CD 25 ( + ) regulatory T ( T ( R ) ) cells , a component of the innate immune response , which play a key role in the maintenance of self tolerance , have become the focus of numerous studies over the last decade . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
This study was designed to determine the role of CD 25 ( + ) CD 4 ( + ) regulatory T ( Tr ) cells in CTL maturation and effector functions using a murine CTL line and in vitro MLC . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Here we found that islet transplantation in the testis , an immunologically privileged site , generates much less memory CD 8 ( + ) T cells but induces more Ag specific CD 4 ( + ) CD 25 ( + ) regulatory T cells than in a conventional site . ^^^ These CD 4 ( + ) CD 25 ( + ) cells exhibited the suppression of alloimmune responses in vivo and in vitro . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Spi B expression is acutely induced coincident with T cell lineage commitment at the CD 4 ( ) CD 8 ( ) CD 44 ( ) CD 25 ( + ) ( DN 3 ) stage of thymocyte development and is then down regulated as thymocytes respond to pre TCR signals and develop beyond the beta selection checkpoint to the CD 4 ( ) CD 8 ( ) CD 44 ( ) CD 25 ( ) ( DN 4 ) stage . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
RESULTS : In rodents , 100 days subsequent to the rejection of the first graft , we observed an increase in blood but not spleen CD 4 ( + ) CD 25 ( + ) T cells , whereas no differences were observed in transcriptional patterns . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
This effect was specifically abrogated by the transfer of neu N derived CD 4 ( + ) CD 25 ( + ) T cells . ^^^ Cyclophosphamide seemed to inhibit regulatory T ( T reg ) cells by selectively depleting the cycling population of CD 4 ( + ) CD 25 ( + ) T cells in neu N mice . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Over the last decade it has become increasingly clear that CD 4 ( + ) CD 25 ( + ) T ( Reg ) cells are crucial for maintenance of T cell tolerance to self antigens in the periphery , and to avoid development of autoimmune disorders . ^^^ Recently , evidence has also emerged that demonstrates that CD 4 ( + ) CD 25 ( + ) T ( Reg ) cells can also suppress T cell responses to foreign pathogens , including viruses such as HIV . ^^^ In this article we review the current knowledge and potential role of CD 4 ( + ) CD 25 ( + ) T ( Reg ) cells in HIV infection . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Predominance of Type 1 cytokines and decreased number of CD 4 ( + ) CD 25 ( +high ) T regulatory cells in peripheral blood of patients with recurrent aphthous ulcerations . ^^^ Moreover , we have found that CD 4 ( + ) CD 25 ( +high ) T regulatory cell proportion was decreased in RAU and represented 3 . 58+ / 0 . 654 % of CD 4 ( + ) T cells in active RAU , 4 . 66+ / 0 . 561 % of CD 4 ( + ) T cells in remission RAU , whereas in healthy controls CD 4 ( + ) CD 25 ( +high ) T cells represented 7 . 30+ / 1 . 238 % of CD 4 ( + ) T cells ( p < 0 . 001 ) . ^^^ Alteration in the number of CD 4 ( + ) CD 25 ( +high ) T regulatory cells in RAU may additionally influence the development of the disease . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
OBJECTIVE : In order to examine whether the target cells of Cy in the immuno augmentative effect are CD 25 ( + ) CD 4 ( + ) regulatory T cells or not , we investigated effect of Cy treatment on CD 25 ( + ) CD 4 ( + ) T cells . ^^^ METHOD : We examined Cy treated CD 25 ( + ) CD 4 ( + ) T cells by flow cytometer and by inhibition assay on proliferation of CD 25 ( ) CD 4 ( + ) T cells . ^^^ RESULTS : Cy treatment remarkably reduced the number and percentage of CD 25 ( + ) CD 4 ( + ) T cells in the spleen and lymph nodes 3 and 5 days later . ^^^ Moreover , CD 25 ( + ) CD 4 ( + ) T cells taken from the Cy treated mice 3 days later showed the lower suppressive activity on proliferation of CD 25 ( ) CD 4 ( + ) T cells , as compared to that from the untreated mice . ^^^ Furthermore , transfer of CD 25 ( + ) CD 4 ( + ) T cells from untreated mice resulted in a significant decrease ( p < 0 . 05 ) of the CH reactions enhanced by Cy treatment . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
BMT resulted in increased numbers of CD 4 ( + ) CD 25 ( bright ) regulatory T cells , increased Foxp 3 expression , a shift in T cell epitope recognition , and a strong reduction of autoantibodies even after rechallenge with MOG . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Naturally arising CD 4 ( + ) CD 25 ( + ) regulatory T ( T ( R ) ) cells are engaged in the maintenance of self tolerance and prevention of autoimmune diseases . ^^^ However , accumulating evidence suggests that a fraction of peripheral CD 4 ( + ) CD 25 ( ) T cells also possesses regulatory activity . ^^^ Here , we identified a subpopulation of CD 4 ( + ) CD 25 ( ) PD 1 ( + ) T cells in the spleen of naive mice that constitutively expressed CTLA 4 and FoxP 3 and was hypoproliferative in response to anti CD 3 antibody stimulation in vitro . ^^^ However , the CD 4 ( + ) CD 25 ( ) PD 1 ( + ) T cells uniquely produced large amounts of IL 4 and IL 10 in response to anti CD 3 and anti CD 28 mAb stimulation , unlike the CD 4 ( + ) CD 25 ( + ) T ( R ) cells . ^^^ The CD 4 ( + ) CD 25 ( ) PD 1 ( + ) T cells exhibited a suppressor activity against the proliferation of anti CD 3 antibody stimulated CD 4 ( + ) CD 25 ( ) PD 1 ( ) T cells in vitro , which was partially abrogated by anti CTLA 4 mAb , but not by anti IL 10 or anti PD 1 mAb . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In addition , T cells ( CD 4 , CD 8 , CD 25 , and gamma / delta TCR ) , B cells ( anti IgM ) , macrophages ( CD 14 ) and granulocytes ( CD 15 ) were quantified . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The tyrosine phosphatase Src homology region 2 domain containing phosphatase 1 ( SHP 1 ) is a known negative regulator of TCR mediated signaling . motheaten mice , lacking expression of SHP 1 , showed a 2 to 3 fold increase in the percentage of CD 4 ( + ) CD 25 ( + ) T ( reg ) cells within the CD 4 ( + ) T cells . ^^^ Our data suggest that the development of CD 4 ( + ) CD 25 ( + ) T ( reg ) cells is intrinsically different from non T ( reg ) cells and that T ( reg ) cells are selectively enriched under conditions of enhanced negative selection . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Although CD 4 ( + ) CD 25 ( + ) cells did not play a prominent role in this suppression , CD 152 expressing T cells played a crucial role in this tolerance . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
An expansion of CD 4 ( + ) CD 25 ( + ) CD62L ( high ) T cells was observed specifically within the LN of tolerant animals , but not in other anatomic sites or under nontolerizing conditions . ^^^ However , administration of FTY 720 , a sphingosine 1 phosphate receptor modulator that induces CD62L independent T cell accumulation in the LNs , restored CD 4 ( + ) CD 25 ( + ) Treg in the LNs along with graft survival . ^^^ These data suggest that alloantigen specific Foxp 3 ( + ) CD 4 ( + ) CD 25 ( + ) Treg develop and are required within the LNs during tolerization , and provide compelling evidence that distinct lymphoid compartments play critical roles in transplantation tolerance . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
GM CSF induced EAT suppression in mice was accompanied by an increase in the frequency of CD 4 ( + ) CD 25 ( + ) regulatory T cells that could suppress mouse thyroglobulin ( mTg ) specific T cell responses in vitro , but the underlying mechanism of this suppression was not elucidated . ^^^ Adoptive transfer of CD 4 ( + ) CD 25 ( + ) T cells from GM CSF treated and mTg primed donors into untreated , but mTg primed , recipients resulted in decreased mTg specific T cell responses . ^^^ Interestingly , in vivo blockade of IL 10R did not affect GM CSF induced expansion of CD 4 ( + ) CD 25 ( + ) T cells . ^^^ Taken together , these results indicated that IL 10 , produced by CD 4 ( + ) CD 25 ( + ) T cells that were probably induced by semimature DCs , is essential for disease suppression in GM CSF treated mice . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Regulatory CD 4 ( + ) CD 25 ( + ) T cells ( Tregs ) suppress autoimmune and inflammatory diseases through mechanisms that are only partly understood . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
At 1 3 wk of infection , relatively high percentages of CD 4 ( + ) CD 25 ( + ) CD 86 ( + ) T cells , as well as high levels of FoxP 3 , TGF beta 1 , and IL 10RI transcripts , were detected in the skin and draining lymph nodes , indicating local accumulation of Treg cells . ^^^ Lesion derived , IL 10 producing CD 4 ( + ) CD 25 ( + ) cells effectively suppressed proliferation and cytokine ( IL 2 and IFN gamma ) production of CD 4 ( + ) CD 25 ( ) effector cells . ^^^ Adoptive transfer of lesion derived CD 4 ( + ) CD 25 ( + ) cells to syngeneic , naive C57BL / 6 mice before infection significantly reduced disease development . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
This protocol activates CD 4 ( + ) CD 25 ( + ) T cells in vivo , which secrete IL 10 upon specific recognition of Tg in vitro and express regulatory T cell ( Treg ) associated markers such as glucocorticoid induced TNFR , CTLA 4 , and Foxp 3 . ^^^ These CD 4 ( + ) CD 25 ( + ) Treg cells suppressed the proliferation and cytokine release of Tg specific , CD 4 ( + ) CD 25 ( ) effector cells in vitro , in an IL 10 independent , cell contact dependent manner . ^^^ Prior adoptive transfer of the same CD 4 ( + ) CD 25 ( + ) Treg cells into CBA / J hosts suppressed Tg induced EAT . ^^^ These results demonstrate that the tolerogenic potential of Tg pulsed , semimature DCs in EAT is likely to be mediated through the selective activation of Tg specific CD 4 ( + ) CD 25 ( + ) Treg cells and provide new insights for the study of Ag specific immunoregulation of autoimmune diseases . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
METHODS : Peripheral blood CD 4 T cell populations were examined for T helper 1 cells ( Th 1 ) , T helper 2 cells ( Th 2 ) , and T Reg by intracellular staining for interferon ( IFN ) gamma and interleukin ( IL ) 4 , and surface staining for CD 25 , respectively . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Prior to disease manifestation , the immunosuppressive function of CD 25 ( + ) CD 4 ( + ) T ( T reg ) cells was impaired in these rats . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
This spontaneous disease in athymic double transgenic mice was prevented by transferring lymph node cells from euthymic mice , but was not prevented when CD 4 ( + ) or CD 25 ( + ) cells were depleted from this population . ^^^ Although purified CD 4 ( + ) CD 25 ( + ) cells scarcely prevented the skin disease induced by adoptive transfer of OT 1 cells , they efficiently prevented the disease when co transferred with CD11c ( + ) dendritic cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The role of CD 4 ( + ) CD 25 ( + ) T regulatory ( T ( reg ) ) cells in priming and expanding virus specific CD 8 ( + ) T cells was investigated . ^^^ CD 4 ( + ) CD 25 ( + ) T ( reg ) cells inhibited anti CD3 / CD28 CD 8 ( + ) T cell proliferation and perforin expression . ^^^ Depletion of CD 4 ( + ) CD 25 ( + ) T ( reg ) cells from chronic HCV patients in vitro increased HCV and EBV peptide driven expansion ( P = 0 . 0005 and P = 0 . 002 , respectively ) and also the number of HCV and EBV specific IFN gamma expressing CD 8 ( + ) T cells . ^^^ Although stimulated CD 8 ( + ) T cells expressed receptors for transforming growth factor beta and interleukin 10 , the presence of antibody to transforming growth factor beta and interleukin 10 had no effect on the suppressive effect of CD 4 ( + ) CD 25 ( + ) regulatory T cells on CD 8 ( + ) T cell proliferation . ^^^ In conclusion , marked CD 4 ( + ) CD 25 ( + ) regulatory T cell activity is present in patients with chronic HCV infection , which may contribute to weak HCV specific CD 8 ( + ) T cell responses and viral persistence . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Here , we investigated the potential role of T cells with a CD 4 ( + ) CD 25 ( + ) regulatory phenotype in suppressing virus specific CD 8 ( + ) T cell proliferation during chronic HCV infection . ^^^ In vitro depletion studies and coculture experiments revealed that peptide specific proliferation as well as gamma interferon production of HCV specific CD 8 ( + ) T cells were inhibited by CD 4 ( + ) CD 25 ( + ) T cells . ^^^ Importantly , CD 4 ( + ) CD 25 ( + ) T cells from persons recovered from HCV infection and from healthy blood donors exhibited significantly less suppressor activity . ^^^ This was associated with a higher frequency of circulating CD 4 ( + ) CD 25 ( + ) cells observed in this patient group . ^^^ Taken together , our results suggest that chronic HCV infection leads to the expansion of CD 4 ( + ) CD 25 ( + ) T cells that are able to suppress CD 8 ( + ) T cell responses to different viral antigens . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) T cells prevent arthritis associated with Borrelia vaccination and infection . ^^^ CD 4 ( + ) CD 25 ( + ) T cells are a population of regulatory T cells associated with control of arthritis in anti interleukin 17 antibody treated Borrelia vaccinated and challenged gamma interferon deficient mice . ^^^ Here , we present direct evidence that adoptive transfer of enriched CD 4 ( + ) CD 25 ( + ) T cells from these mice can prevent the development of arthritis in Borrelia vaccinated and challenged mice . ^^^ These findings establish a major role for CD 4 ( + ) CD 25 ( + ) T cells in the prevention of arthritis in Borrelia vaccinated and challenged animals . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Here , we show that this polymorphism is associated with the frequency of CD 4 ( + ) CD 25 ( + ) regulatory T cells in healthy human volunteers . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Transfer of CD 4 ( + ) CD45RB ( high ) T cells into RAG ( / ) mice causes colitis , which can be prevented by CD 4 ( + ) CD 25 ( + ) regulatory T cells ( Treg ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Naturally occurring CD 4 ( + ) CD 25 ( + ) regulatory T cells ( Treg ) are crucial in immunoregulation and have great therapeutic potential for immunotherapy in the prevention of transplant rejection , allergy , and autoimmune diseases . ^^^ To this end , freshly isolated CD 4 ( + ) CD 25 ( + ) Treg were labeled with CFSE and stimulated with ( target ) alloantigen and IL 2 plus IL 15 in short term cultures . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We went on to show that allogeneic T cells ( including CD 4 ( + ) CD45RO , CD 4 ( + ) CD45RA , and CD 4 ( + ) CD 25 ( ) subsets ) were anergized following exposure to vitamin treated DCs , and secreted higher levels of Th 2 cytokines and IL 10 than cells incubated with control DCs . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Murine CD 4 ( + ) CD 25 ( + ) T regulatory ( Treg ) cells were cocultured with CD 4 ( + ) CD 25 ( ) Th cells and APCs or purified B cells and stimulated by anti CD 3 mAb . ^^^ Replacement of APCs by B cells did not significantly affect the suppression of CD 4 ( + ) CD 25 ( ) Th cells . ^^^ When IL 4 was added to separate cell populations , this cytokine promoted CD 4 ( + ) CD 25 ( ) Th and CD 4 ( + ) CD 25 ( + ) Treg cell proliferation , whereas the suppressive competence of CD 4 ( + ) CD 25 ( + ) Treg cells was preserved . ^^^ Conversely , IL 4 added to coculture of APCs , CD 4 ( + ) CD 25 ( ) Th cells , and CD 4 ( + ) CD 25 ( + ) Treg cells inhibited the suppression of CD 4 ( + ) CD 25 ( ) Th cells by favoring their survival through the induction of Bcl 2 expression . ^^^ When naive CD 4 ( + ) CD 25 ( ) Th cells were replaced by Th 1 and Th 2 cells , cell proliferation of both subsets was equally suppressed , but suppression was less pronounced compared with that of CD 4 ( + ) CD 25 ( ) Th cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Moreover , Sle1a and Sle1c were associated with a significantly reduced level of CD 4 ( + ) CD 25 ( + ) regulatory T cells that precedes autoantibody production , suggesting a causal relationship with the generation of autoreactive T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) regulatory T cells are increasingly recognized as central players in the regulation of immune responses . ^^^ Little is known about the ability of CD 4 ( + ) CD 25 ( + ) regulatory T cells to suppress antigen specific immune responses in humans . ^^^ It has been previously shown that CD 4 ( + ) CD 25 ( + ) regulatory T cells anergize CD 4 ( + ) T cells and turn them into suppressor T cells . ^^^ In the present study we demonstrate for the first time in humans that CD 4 ( + ) CD 25 ( + ) T cells are able to inhibit the proliferation and cytokine production of antigen specific CD 4 ( + ) and CD 8 ( + ) T cells . ^^^ This suppression only occurs when CD 4 ( + ) CD 25 ( + ) T cells are preactivated . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
UV induced regulatory T cells have been recently characterized to express CD 4 and CD 25 and to release the immunosuppressive cytokine interleukin 10 upon activation . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Immunosuppression mediated by CD 4 ( + ) CD 25 ( + ) regulatory T cells has been shown to ameliorate such pathogenic immune responses in animal models . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The discovery that CD 4 T cells with the CD 25 surface marker were enriched in suppressor activity enabled further phenotypic and functional analysis of the so called natural suppressor cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Engrafted ATL cells were dually positive for human CD 4 and CD 25 , and displayed patterns of HTLV 1 integration identical to those of donors by Southern blot analysis . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) regulatory T cells ( Treg ) are important mediators of peripheral immune tolerance ; however , whether Treg participate also in hepatic immune tolerance is not clear . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The number of activated memory T cells ( CD 4 ( + ) CD 8 ( + ) CD 25 ( + ) cells ) increased slightly in the peripheral blood mononuclear cell ( PBMC ) population soon after vaccination , then diminished within a few weeks . ^^^ The number of activated cytotoxic T cells ( CD 4 ( ) CD 8 ( + ) CD 25 ( + ) cells ) peaked approximately 2 weeks after the memory population . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
UV induced regulatory T cells express CD 4 and CD 25 and upon activation release the immunosuppressive cytokine interleukin 10 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 25 ( + ) CD 4 ( + ) T cells , but not CD 25 ( ) CD 4 ( + ) T cells , showed a fivefold increase in IFN gamma mRNA expression within 24 h of re encountering alloantigen in vivo . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Therefore , we examined whether CD 25 ( + ) CD 4 ( + ) regulatory T ( Treg ) cells might be involved in a inhibition of CD 8 ( + ) T cell priming or in the modulation of the magnitude of the ' peak ' antiviral CD 8 ( + ) T cell response primed by DNA immunization . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Regulatory CD 4 ( + ) CD 25 ( + ) T cells are important components of the homeostasis of the immune system , as impaired CD 4 ( + ) CD 25 ( + ) T cell activity can cause autoimmune diseases and allergy . ^^^ The mechanism of suppression by CD 4 ( + ) CD 25 ( + ) T cells remains controversial ; different in vivo and in vitro studies raise possible roles for the immunosuppressive cytokines interleukin 10 and transforming growth factor beta , forkhead transcription factor Foxp 3 , glucocorticoid induced tumor necrosis factor receptor , cytotoxic lymphocyte associated antigen 4 , 4 1BB costimulator receptor , a CD 4 related molecule LAG 3 , and neuropilin 1 . ^^^ Current data suggest that Th 2 responses to allergens are normally suppressed by CD 4 ( + ) CD 25 ( + ) T cells . ^^^ Suppression by CD 4 ( + ) CD 25 ( + ) T cells is decreased in allergic individuals . ^^^ Furthermore , CD 4 ( + ) CD 25 ( + ) T cells play a key role in regulating airway eosinophilic inflammation . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
A cross sectional study with 39 relapsing remitting MS patients demonstrated that the percentage of CD 25 ( + ) CD45RO ( + ) CD 4 ( + ) CD 3 ( + ) cells was significantly increased in peripheral blood as well as in CSF of active MS patients compared with inactive MS patients . ^^^ A longitudinal study with 11 relapsing remitting MS patients also showed a higher percentage of CD 25 ( + ) CD45RO ( + ) CD 4 ( + ) CD 3 ( + ) cells in peripheral blood at the phase of exacerbation than during remission . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In this study , we show that exposure to the L 3 of Brugia also induces the expansion of a population of CD 4 cells that express CD 25 and cytotoxic T lymphocyte associated antigen 4 in an IL 4 independent fashion . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Immunohistological analysis of CD 4 , CD 25 , and CTLA 4 and the gene expression analysis of FOXP 3 , TGF beta 1 , and IL 10 on gingival biopsies revealed the presence of CD4+CD25+ Tr cells in all tissues . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) FoxP 3 ( + ) regulatory T cells ( T ( reg ) ) suppress T cell function and protect rodents from autoimmune disease . ^^^ In contrast , TCR restimulation of T ( reg ) in vitro revealed a reduced sensitivity toward activation induced cell death compared with CD 4 ( + ) CD 25 ( ) T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
However , normal numbers of endogenous naive CD 4 T cells , but not CD 25 ( + ) CD 4 ( + ) T regulatory cells , efficiently suppress this expansion in vivo . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Like CD 4 ( + ) CD 25 ( + ) T cells , CD 8 ( + ) CD 25 ( + ) T cells appear late in the periphery during ontogeny . ^^^ Peripheral CD 8 ( + ) CD 25 ( + ) T cells from MHC class 2 deficient mice also share phenotypic and functional features with regulatory CD 4 ( + ) CD 25 ( + ) T cells : in particular , they strongly express glucocorticoid induced TNFR family related gene , CTLA 4 and Foxp 3 , produce IL 10 , and inhibit CD 25 ( ) T cell responses to anti CD 3 stimulation through cell contacts with similar efficiency to CD 4 ( + ) CD 25 ( + ) T cells . ^^^ However , unlike CD 4 ( + ) CD 25 ( + ) T cells CD 8 ( + ) CD 25 ( + ) T cells from MHC class 2 deficient mice strongly proliferate and produce IFN gamma in vitro in response to stimulation in the absence of exogenous IL 2 . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The receptor is preferentially expressed on CD 4 ( + ) CD 25 ( + ) regulatory T cells and GITR signals break the suppressive activity of the subset . ^^^ A single injection of anti GITR mAb ( DTA 1 ) immediately after viral infection significantly increased the number of CD 4 ( + ) and CD 8 ( + ) T cells expressing CD 25 , an activation surface marker , and secreting IFN gamma . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Leukocyte CD40L deficiency affects the CD 25 ( + ) CD 4 T cell population but does not affect atherosclerosis . ^^^ These BM chimeras were analysed by flow cytometry for the presence of innate Tregs ( CD45RB ( low ) CD 25 ( + ) CD 4 ) in lymphoid organs and peripheral blood . ^^^ As in CD 40 ( / ) mice , the CD45RB ( high ) : CD45RB ( low ) CD 4 T cell ratio significantly increased and the CD 25 ( + ) CD 4 ( + ) subpopulation significantly decreased in LDLR ( / ) mice receiving CD40L ( / ) BM compared to LDLR ( / ) mice receiving wild type BM . ^^^ In conclusion , the present study shows that CD 40 CD40L interactions on leukocytes are essential for the size of the CD45RB ( low ) CD 25 ( + ) CD 4 Treg subpopulation . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In this study , we expand upon those observations by demonstrating an alternative pathway by which IL 12 could promote Th 1 inflammatory responses in mice , namely , by restoring proliferation and cytokine expression by effector T cells in the presence of CD 4 ( + ) CD 25 ( + ) regulatory T cells ( Treg ) . ^^^ Our studies suggest that IL 12 could act in concert with other proinflammatory factors to stimulate CD 4 ( + ) CD 25 ( ) T cell activation in the presence of Treg . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Although it has been shown that CD 4 ( + ) CD 25 ( + ) regulatory T cells ( T ( reg ) ) contribute to long term graft acceptance , their impact on the effector compartment and the mechanism by which they exert suppression in vivo remain unresolved . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The development of organ specific autoimmune diseases in mice thymectomized on day 3 of life ( d3tx mice ) can be prevented by transferring CD 4 ( + ) CD 25 ( + ) T cells from syngeneic , normal adult mice . ^^^ Using a d3tx model , we asked whether CD 4 ( + ) CD 25 ( + ) T cell deficiency contributes to glomerulonephritis ( GN ) in lupus prone mice . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We have also found that Sle1c was associated with the production of histone specific autoreactive CD 4 ( + ) T cells , which correlated with higher activation and proliferative responses , and a reduction in the CD 4 ( + ) CD 25 ( + ) CD62L ( + ) forkhead / winged helix transcription factor gene ( Foxp 3 ( + ) ) compartment . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
This proliferation was associated with a low proportion of CD 4 ( + ) CD 25 ( + ) T cells and a high proportion of dendritic cells in the MLN of BBdp rats . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
BACKGROUND AND AIMS : CD 4 T lymphocytes constitutively expressing the IL 2 receptor alpha chain ( CD 25 ) ( T regs ) are central to self tolerance maintenance , preventing the proliferation and effector function of autoreactive T cells . ^^^ T regs were purified from PBMCs by CD 4 negative selection followed by CD 25 positive selection , using immunomagnetic beads . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Here we show that analysis of CD 25 expression in human circulating CD 4 T lymphocytes with respect to their in vivo differentiation stages identifies a distinct subset of CD25CCR7CD62LCTLA 4FOXP3 cells contained in the CD45RA / RO naive fraction . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The impact of HIV infection on regulatory CD 4 ( + ) CD 25 ( high ) ( Treg ) lymphocyte subpopulations was evaluated by FOXP 3 quantitative reverse transcriptase polymerase chain reaction and by flow cytometry . ^^^ The frequency of CD 4 ( + ) CD 25 ( high ) lymphocytes , measured by flow cytometry , was decreased in HIV patients ( n = 38 ) compared with the group of uninfected subjects ( n = 39 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
However , the percentage of CD 4 ( + ) CD 25 ( + ) T cells was significantly greater in both IEL and LPL from random source animals . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The increase a number of splenic CD 4 ( + ) CD 25 ( + ) T cells and decreased synthesis of the Th 1 cytokine IL 2 also occurred . ^^^ These studies show , for the first time , that agonistic anti 4 1BB mAb can improve experimental colitis by reduction of IL 2 and augmentation of CD 4 ( + ) CD 25 ( + ) regulatory T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Relative to the pokeweed mitogen induced responses of CD 4 ( + ) cells from steers ( 5 to 6 mo of age ) , CD 4 ( + ) cells from 1 wk old calves showed decreased proliferative activity , delayed increase in CD 25 expression , and no demonstrable increase in CD 44 expression or decrease in CD62L expression . ^^^ Feeding calves an intensified milk replacer was associated with decreased proliferation of mitogen stimulated CD 4 ( + ) , CD 8 ( + ) , and gammadeltaT cell receptor ( + ) cells ; decreased CD 25 expression by mitogen stimulated CD 4 ( + ) and CD 8 ( + ) cells ; and decreased CD 44 expression by mitogen stimulated CD 8 ( + ) cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In addition , the footpad injection of flagellin or LPS induced significant CD 4 ( + ) T cell activation in the draining popliteal lymph node , as judged by increased CD 69 or CD 25 expression . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
OBJECTIVE : Regulatory CD 4 T cells that express high levels of CD 25 play a vital role in the maintenance of tolerance to self antigens and are required for the induction of nonresponsiveness to alloantigens . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The examinations were performed in FACScan flow cytometer with the use of wide set of monoclonal antibodies : CD 3 , CD 4 , CD 8 , TCRalphabeta , TCRgammadelta , CD 19 , CD 25 , CD45RA , CD45RO , CD 69 , HLA DR , CD 16 and CD 56 , which particularly allowed detailed analysis of T lymphocytes . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) T cells and CD 25 ( + ) ( IL 2R ) T cells were stimulated the most by 85A and 85B , while the 35 kDa protein primarily stimulated CD 21 ( + ) B cells involved in humoral immune responses . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
FoxP 3 ( + ) CD 4 ( + ) CD 25 ( + ) T cells with regulatory properties can be cultured from colonic mucosa of patients with Crohn ' s disease . ^^^ Summary CD 4 ( + ) CD 25 ( + ) regulatory T cells ( T ( regs ) ) are involved in the maintenance of peripheral tolerance and ensure a balanced immune response competent of fighting pathogens and at the same time recognizing commensals as harmless . ^^^ We cultured biopsies of colonic mucosa in the presence of high concentrations of interleukin ( IL ) 2 and IL 4 to overcome the anergic nature of naturally occurring CD 4 ( + ) CD 25 ( + ) T ( regs ) in the mucosa . ^^^ We investigated the expression of FoxP 3 and regulatory potential of gut derived CD 4 ( + ) CD 25 ( + ) T cells cultured from patients with CD and healthy individuals . ^^^ The FoxP 3 expression was analysed by reverse transcriptase polymerase chain reaction ( RT PCR ) , and the suppressive effect of FoxP 3 ( + ) CD 4 ( + ) CD 25 ( + ) T cells on proliferation and cytokine production of autologous CD 4 ( + ) T cells was assessed by flow cytometry . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The enhancement in IgE production after GEN exposure in females but not in males was associated with decreases in the percentages of CD 4 ( + ) CD 25 ( + ) T suppressor cells , and increases in the natural killer ( NK ) cell activity , the basal splenocyte proliferation , the expression of CD 86 by B cells , and the production of IL 2 and IL 4 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Much focus has been placed on thymically derived CD 4 ( + ) CD 25 ( + ) regulatory T cells , given that the depletion of this subset in murine models results in the spontaneous development of autoimmune diseases . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Activated natural killer cells were preferentially recruited by supernatants of virus activated PDC , and supernatants of CD40L activated PDC attracted memory CD 4 ( + ) T cells , particularly the CD 4 ( + ) CD45RO ( + ) CD 25 ( + ) T cells described for their regulatory activities . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The expressions of CD 4 , CD 8 , CD 25 , and CLA of metal specific T cells were determined by flow cytometric analysis ( FACS ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Our data indicate that patients with RRMS , treated with IFN beta1a , exhibited a significant increase in CD 4 ( + ) CD 25 ( ++ ) T suppressor cells , accompanied by a significant decrease in cytotoxic lymphocytes ( CD 8 ( + ) CD 28 ( ) and natural killer [ NK ] cells ) and IFN gamma production , which could both contribute to an explanation of the previously described beneficial effects of IFN beta treatment in MS . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
METHODS : Five OCT specimens obtained from patients with active GO and five control specimens obtained from forensic cadavers who died from nonmedical reasons were stained with anti CD 3 , CD 4 , CD 8 , CD45RO , HLA Dr , CD 25 , and TNF alpha monoclonal antibodies . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
AdIL 23 pretreatment resulted in increased numbers of CD 4 ( + ) CD 25 ( + ) activated T cells in lungs and draining lymph nodes compared to control groups and more CD 4 ( + ) T cells bearing surface memory markers in lung lymph nodes . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In this study , TGF beta 1 production was demonstrated in peripheral blood mononuclear cells from healthy subjects never exposed to leishmaniae in response to live Leishmania guyanensis , and the TGF beta 1 producing cells were described as a distinct subpopulation of CD 4 ( + ) CD 25 ( + ) regulatory T cells . ^^^ The suppressive properties of CD 4 ( + ) CD 25 ( + ) T cells were demonstrated in vitro by their inhibition of production of interleukin 2 ( IL 2 ) and IL 10 by CD 4 ( + ) CD 25 ( ) T cells in the presence of either anti CD 3 or L . guyanensis . ^^^ Although neutralization of TGF beta 1 did not reverse the suppressive activity of CD 4 ( + ) CD 25 ( + ) T cells activated by anti CD 3 , it reversed the suppressive activity of CD 4 ( + ) CD 25 ( + ) T cells activated by L . guyanensis . ^^^ Altogether our data demonstrated that TGF beta 1 is involved in the suppressive activity of L . guyanensis stimulated CD 4 ( + ) CD 25 ( + ) T cells from healthy controls . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
After 24 48 h exposure to FNf , activated T cells and monocytes formed clusters displaying CD 25 , CD 14 , CD 3 , and CD 4 that were not dissociable by chelation of divalent cations . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
These TSLP conditioned dendritic cells are then able to induce the proliferation and differentiation of CD 4 ( + ) CD 8 ( ) CD 25 ( ) thymic T cells into CD 4 ( + ) CD 25 ( + ) FOXP 3 ( + ) ( forkhead box P 3 ) regulatory T cells . ^^^ These findings suggest that Hassall ' s corpuscles have a critical role in dendritic cell mediated secondary positive selection of medium to high affinity self reactive T cells , leading to the generation of CD 4 ( + ) CD 25 ( + ) regulatory T cells within the thymus . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Most of the cells were T helper 2 , although 10 . 4 % + / 1 . 4 % were of the T regulatory ( T ( reg ) ) phenotype , ie , CD 4 ( + ) CD 25 ( + ) . ^^^ Both the engrafted donor CD 4 ( + ) CD 25 ( ) and CD 4 ( + ) CD 25 ( + ) cells induced comparable in vitro suppression of T cell proliferation , thus suggesting their role in the persistence of the donor T cells in vivo . ^^^ The CD 4 ( + ) CD 25 ( ) cells produced interleukin 10 or interleukin 4 and were inhibited by anti T helper 2 cytokine neutralizing antibodies , whereas the CD 4 ( + ) CD 25 ( + ) cells showed no evidence of any involvement of a cytokine like soluble mediator and expressed cytotoxic T lymphocyte antigen 4 ( CTLA 4 ) and foxp 3 constitutively . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Naturally occurring CD 4 ( + ) CD 25 ( + ) T ( reg ) ( nT ( reg ) ) cells , which typically display potent inhibitory effects on T cell functions in vitro and in vivo , may be defective at controlling autoimmunity in T1D . ^^^ We have examined the relative contribution of CD 4 ( + ) CD 25 ( + ) nT ( reg ) cells in the immune regulation of T1D in the NOD mouse model . ^^^ CD 4 ( + ) CD 25 ( + ) T cells represent 5 10 % of CD 4 ( + ) thymocytes or peripheral T cells from prediabetic neonatal NOD mice , are anergic to TCR signals , and potently suppress activated T cells in a contact dependent and cytokine independent fashion in vitro . ^^^ Unlike total CD 4 ( + ) T cells , prediabetic CD 25 ( + ) depleted CD 4 ( + ) T cells are potently diabetogenic when transferred in immunodeficient NOD mice . ^^^ Co transfer of CD 4 ( + ) CD 25 ( + ) T cells from thymocytes or peripheral lymphoid tissues of neonatal NOD mice dramatically halts disease development and beta islet cell lymphocytic infiltration , even when T1D is induced by CD 4 ( + ) T cells from BDC2 . 5 transgenic or diabetic NOD mice . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
T regulatory CD 4 ( + ) CD 25 ( + ) cells play a role in maintaining self tolerance by downregulating Th 1 induced proinflammation . ^^^ Annexin 5 binding on CD 4 ( + ) CD 25 ( + ) was assessed using three color assay by flow cytometry . ^^^ The absolute number of CD 4 ( + ) CD 25 ( + ) cells / mL in the peripheral blood at baseline was reduced in 11 out of 17 active RA patients when compared with that of the control group ( 24 + / 7 vs . 32 + / 11 , respectively ; P = 0 . 02 ) . ^^^ Following anti TNF alpha therapy , CD 4 ( + ) CD 25 ( + ) cell counts of patients were equivalent to those of normals . ^^^ CD 4 ( + ) CD 25 ( + ) T regulatory cells display increased proclivity to undergo spontaneous apoptosis in active RA . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
This physiological repair mechanism is controlled by naturally occurring CD 4 ( + ) CD 25 ( + ) regulatory T cells ( Treg cells ) , with an on / off switch regulated by brain derived compounds . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The findings show for the first time in vivo and in fetal thymus organ culture ( FTOC ) that TGF beta is not required for thymocytes to differentiate along the entire pathway of thymic T cell development , as defined by the expression patterns of CD 4 , CD 8 , CD 25 , and CD 44 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The difference and similarity between bovine activated CD 8 ( + ) T cells ( CD 8 ( + ) CD 26 ( + ) ) and well established human CD 4 ( + ) CD 25 ( + ) T regulatory ( Tr ) cells may help to reveal their unique immune regulatory system in the host infected with S . aureus . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
MT 110 induced a costimulation independent polyclonal activation of CD 4 and CD 8 positive T cells as seen by de novo expression of CD 69 and CD 25 , and secretion of interferon gamma , tumor necrosis factor alpha , and interleukins 2 , 4 and 10 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In the current review , we provide evidences indicating that TIM 3 is capable of modulating the function of CD 4 ( + ) CD 25 ( + ) regulatory T cells and inhibiting aggressive Th 1 mediated auto and allo immune responses . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
BACKGROUND : Active suppression by CD 4 ( + ) CD 25 ( + ) regulatory T lymphocytes plays an important role in the downregulation of T cell responses to foreign and self antigens . ^^^ OBJECTIVE : To analyze whether the CD 4 ( + ) CD 25 ( + ) regulatory T lymphocytes exist and function normally in malignant pleural effusion . ^^^ METHODS : The percentages of CD 4 ( + ) CD 25 ( + ) T lymphocytes in pleural effusion and peripheral blood from patients with lung cancer with malignant effusion , pleural lavage and peripheral blood from patients with lung cancer without effusion , and peripheral blood from healthy control subjects were determined by flow cytometry . ^^^ CD 4 ( + ) CD 25 ( + ) and CD 4 ( + ) CD 25 ( ) T cells from pleural effusion and peripheral blood were isolated , and were cultured to observe the effects of CD 4 ( + ) CD 25 ( + ) cells on proliferation response of CD 4 ( + ) CD 25 ( ) T cells in vitro . ^^^ MAIN RESULTS : There were increased numbers of CD 4 ( + ) CD 25 ( + ) T cells in malignant pleural effusion from patients with lung cancer compared with pleural lavage from patients with lung cancer without pleural effusion , and that these cells have constitutive high level expression of Foxp 3 and cytotoxic lymphocyte associated antigen 4 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Patients with PD had significantly decreased CD4+ : CD 8 ( + ) T cell ratios ( P < 0 . 001 ) , fewer CD 4 ( + ) CD 25 ( + ) T cells ( P < 0 . 01 ) , and significantly increased ratios of IFN gamma producing to IL 4 producing T cells ( P < 0 . 001 ) . ^^^ The characteristics of predominant expression of CD 8 ( + ) T cells , depletion of CD 4 ( + ) CD 25 ( high ) cells , and a shift to a T ( H ) 1 type immune response in the peripheral immune system in PD patients may reflect an immune reaction associated inflammatory process in the brain . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Additionally , evaluation of CD 25 ( + ) and CD 44 ( + ) expression by thymocytes indicated that the decrease in the percentage of CD 44 ( + ) CD 25 ( + ) thymocytes was at least partially responsible for the decrease in the percentage of CD 4 ( ) CD 8 ( ) thymocytes in F ( 1 ) male mice . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Four color flow cytometry was utilized to simultaneously identify three major porcine T cell surface markers ( CD 4 , CD 8 , and gammadelta TCR ) and detect activation marker CD 25 ( alpha chain of IL 2 receptor ) or intracellular IFNgamma . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Expression of CD 25 and CD 26 was increased ( P < 0 . 05 ) on CD 4 ( + ) , CD 8 ( + ) , and gammadelta T cell receptor positive cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Such tumor induced regulatory T cells ( TMTregs ) arose both from precommitted `` natural ' ' regulatory T cells and CD 4 ( + ) CD 25 ( ) GITR ( low ) precursors . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
By contrast , HGMC did not express detectable amounts of CD 1 , CD 2 , CD 4 , CD 5 , CD 14 , CD 15 , CD 16 , CD 22 , CD 24 , CD 25 , CD 26 , CD 27 , CD 28 , CD 31 , CD 32 , CD 34 , CD 35 , CD 88 , or CD 116 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Subsets of CD 4 ( + ) T cells with regulatory capacity , such as CD 25 ( + ) Tregs , have been identified in mice and man , and recent observations suggest that in RA , the function of these regulatory T cells is severely impaired . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Elimination of CD 4 ( + ) CD 25 ( + ) T cell accelerates the development of glomerulonephritis during the preactive phase in autoimmune prone female NZB 10 NZW F mice . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Mice with a defect in Foxp 3 , required for the generation and activity of CD 4 ( + ) CD 25 ( + ) regulatory T cells , exhibit massive lymphoproliferation and severe inflammatory infiltration of multiple organs , in particular the lungs , liver , and skin . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The CD 4 ( + ) CD 25 ( + ) Foxp 3 ( + ) Treg subset develops in the thymus , can be induced in the periphery during the course of normal immune responses and utilizes a T cell repertoire skewed towards autoantigens . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In contrast with other Tregs models , DSBT Tregs activity is not restricted to CD 4 ( + ) CD 25 ( + ) but to CD 4 ( + ) CD45RC ( ) cells , whereas CD 4 ( + ) CD45RC ( + ) cells act as effector cells and accelerate rejection . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The regulatory activity generated in tumor draining LNs exclusively belonged to the CD 4 ( + ) T cell subpopulation that expresses both CD 25 and a high level of CD62L . ^^^ Forkhead / winged helix transcription factor gene expression was detected only in the CD62L ( high ) CD 4 ( + ) CD 25 ( + ) T cells . ^^^ CD62L ( high ) CD 4 ( + ) CD 25 ( + ) Treg and CD62L ( low ) CD 4 ( + ) CD 25 ( + ) T cells , which possess effector T cell functions , had comparable expression of LFA 1 , VLA 4 , CTLA 4 , lymphocyte activation gene 3 , and glucocorticoid induced TNFR . ^^^ Thus , only CD62L expression could distinguish regulatory CD 4 ( + ) CD 25 ( + ) cells from effector CD 4 ( + ) CD 25 ( + ) cells in draining LNs as a surface marker . ^^^ CD62L ( high ) CD 4 ( + ) CD 25 ( + ) Treg contained a subpopulation that expressed CD 86 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In the study of human regulatory cells , we have found that the cells that consistently demonstrate the in vitro regulatory activity most similar to that described for murine cells in vitro are best identified by restricting the isolation of CD25+ CD 4 T cells to those cells expressing only the highest levels of CD 25 , representing approximately 2 3 % of total CD 4 T cells . ^^^ Furthermore , the cells expressing medium to low levels of CD 25 not only do not exhibit suppressive activity directly ex vivo , but also actually contain a significant proportion of CD62L CD 4 T cells which are believed to be in vivo activated T cells . ^^^ Due to the inherent difficulties in using CD 25 as a marker for the purification of Treg cells , the finding that selection of the CD25high subset of CD4+ CD25+ T cells minimizes the co isolation of contaminating activated CD 4 T cells is important for future studies of these Treg cells in human disease . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 25 expressing donor T cells may be involved in the prevention or induction of GvHD as these cells comprise both CD 4 +CD25+ regulatory T ( Treg ) cells and preactivated CD4+ or CD8+ conventional T cells . ^^^ As Treg cells are often identified within the CD 4 +CD25hiCD45RB low subset , we determined the frequencies of CD4+ and CD8+ T cells with different expression levels of CD 25 and CD45RB . ^^^ RESULTS : The frequencies or infused doses of donor CD 4 +CD25+ T cells , regardless of their CD 25 and CD45RB expression levels , showed no correlation with the clinical outcome in univariate and multivariate analyses . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) regulatory T ( T ( reg ) ) cells have a crucial role in maintaining immune tolerance . ^^^ CD 4 ( + ) CD 25 ( hi ) cells underwent homeostatic peripheral expansion during immune reconstitution , and in lymphopenic individuals receiving IL 2 , the T ( reg ) cell compartment was markedly increased . ^^^ These studies suggest that IL 2 and lymphopenia are primary modulators of CD 4 ( + ) CD 25 ( + ) T ( reg ) cell homeostasis . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The frequency of T cell clones and gingival tissues expressing FOXP 3 , a possible master gene for mouse CD 4 ( + ) CD 25 ( + ) regulatory T cells , was very high ( 97 % , 93 % , and 100 % for gingival T cell clones , peripheral blood T cell clones , and gingival tissues , respectively ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Interleukin 2 signaling is believed to be critically involved in several aspects of CD 25 ( + ) CD 4 ( + ) regulatory T cell biology , such as intrathymic development , peripheral survival and suppressive function . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Of the CD 4 ( + ) T cells , 59 . 6+ / 3 . 9 % expressed the marker for the regulatory phenotype , CD 4 ( + ) CD 25 ( + ) . ^^^ About 43 . 6+ / 3 . 8 % CD 4 ( + ) CD 25 ( + ) subset co expressed both the CD 152 and FOXP 3 , the Treg associated molecules . ^^^ A positive correlation was found between the presence of CD 4 ( + ) CD 25 ( + ) subset and age ( > or =50 years old ) ( r=0 . 51 ; p=0 . 045 ) . ^^^ However , no significant correlation between tumour stage and CD 4 ( + ) CD 25 ( + ) T cells was found . ^^^ In addition , we also found that the CD 4 ( + ) CD 25 ( ) subset correlated with the expression of the nuclear oestrogen receptor ( ER ) alpha in the tumour cells ( r=0 . 45 ; p=0 . 040 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Plastic adhesion decreased the macrophage numbers by approximately 30 % and CD 4 ( + ) CD 25 ( + ) cells by about 60 % . ^^^ This was accompanied by increased medium levels of TNFalpha , IFNgamma and IL 10 , which suggest that either CD 4 ( + ) CD 25 ( + ) cells , macrophages , or both , down regulate production of both Th 1 and certain Th 2 cytokines . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Congenital deficiency of CD 4 ( + ) CD 25 ( + ) Treg cells caused by loss of function mutations in the gene encoding Foxp 3 triggers a syndrome of lymphoproliferation and myeloproliferation , autoimmunity , and allergic dysregulation , whereas deficient allergen specific Treg cell responses have been associated with a number of allergic and autoimmune disorders . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Both naturally occurring CD 4 ( + ) CD 25 ( + ) T ( Reg ) cells and inducible populations of allergen specific IL 10 secreting T ( R ) 1 cells inhibit allergen specific effector cells in experimental models . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The transcription factor Foxp 3 is a master switch gene that controls the development and function of natural and adaptive CD 4 ( + ) CD 25 ( + ) regulatory T ( T ( R ) ) cells . ^^^ CONCLUSION : Allergic dysregulation is a common and fundamental consequence of loss of CD 4 ( + ) CD 25 ( + ) T ( R ) cells caused by Foxp 3 deficiency in different species . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
MLNC from infected animals contained elevated numbers of CD 4 ( + ) CD 25 ( + ) Foxp 3 ( + ) T cells , higher TGF beta expression , and produced strong interleukin ( IL ) 10 responses to parasite antigen . ^^^ Suppression was associated with CD 4 ( + ) T cells from MLNC , with the CD 4 ( + ) CD 25 ( + ) marker defining the most active population . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
A considerable increase in the absolute and relative amount of lymphocytes with markers CD 3 , CD 4 , CD 16 , CD 21 , CD 25 , a rise in the levels of IgA , IgG and a decrease in the level of total IgE in the blood serum were established . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
MATERIAL AND METHODS : Immunological investigation of 136 IHD patients ( 102 with stable angina and 34 with myocardial infarction ) included the study of the phenotype of lymphocytes : CD 3 , CD 4 , CD 8 , CD 16 , CD 19 , CD11 / 18 , CD 25 , CD 95 and HLA DR . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We studied the influence of CD 4 ( + ) CD 25 ( + ) regulatory T cells ( Treg ) on the ability of suboptimal T cell receptor ligands to provoke autoimmunity . ^^^ By using CD 4 ( + ) T cell driven experimental autoimmune encephalomyelitis as a model , it was found that depletion of CD 4 ( + ) CD 25 ( + ) Foxp 3 ( + ) Treg allowed pathology to develop in response to suboptimal T cell stimulation . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Foxp 3 is required for the generation and activity of CD 4 ( + ) CD 25 ( + ) regulatory T ( T reg ) cells , which are important controllers of autoimmunity , including type 1 diabetes . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Increased population of CD 4 ( + ) CD 25 ( high ) , regulatory T cells with their higher apoptotic and proliferating status in peripheral blood of acute myeloid leukemia patients . ^^^ In this study we investigated the possible involvement of CD 4 ( + ) CD 25 ( high ) T reg in the immune impairment of patients with acute myeloid leukemia ( AML ) . ^^^ EXPERIMENTAL DESIGN : The frequencies and phenotypes of CD 4 ( + ) CD 25 ( high ) T cells in the peripheral blood of AML patients were determined by flow cytometry . ^^^ To assess the functional activity of CD 4 ( + ) CD 25 ( high ) T cells , CD 4 ( + ) CD 25 ( high ) , and CD 4 ( + ) CD 25 ( ) T cells were sorted from peripheral blood mononuclear cells with FACS Vantage . ^^^ The immunoregulatory properties of CD 4 ( + ) CD 25 ( high ) and CD 4 ( + ) CD 25 ( ) T cells were characterized by proliferation assays and cytokine production assays . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
E 2 increases the number and activity of FoxP 3 ( + ) T cells through Esr 1 signaling during TCR activation of CD 4 ( + ) CD 25 ( ) T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We found increased ex vivo percentages of CD 4 ( + ) CD 25 ( hi ) and CD 4 ( + ) CD 25 ( + ) CTLA 4 ( + ) cells and increased interleukin ( IL ) 10 responses to parasite Ag in vitro in CBMCs from neonates born to mothers with placental P . falciparum infection at delivery . ^^^ Depleting CBMCs of CD 4 ( + ) CD 25 ( + ) cells before cell culture led to the abrogation of parasite Ag specific IL 10 responses , to enhanced interferon gamma responses , and to enhanced expression of CD 25 on CD 8 ( + ) T cells and of major histocompatibility complex class 1 and 2 on monocytes . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Regulatory T cells ( TREGs ) constitutively expressing CD 4 , CD 25 , and the transcription factor Foxp 3 can prevent a wide range of experimental and spontaneous autoimmune diseases in mice . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In this study , we investigated if cord blood ( CB ) CD 4 ( + ) CD 25 ( + ) T cells play a significant role in reducing the immune responses of allo reactive CD 4 ( + ) CD 25 ( ) T lymphocytes . ^^^ METHODS : We compared CB CD 4 ( + ) CD 25 ( ) and CD 4 ( + ) CD 25 ( + ) T cells , either nave or antigenic stimulated , to their counterparts in unmobilized adult peripheral blood ( APB ) with respect to genetic expression patterns , immunophenotype , suppressive activity , and mechanism ( s ) of suppression . ^^^ RESULTS : Both nave CB and APB CD 4 ( + ) CD 25 ( + ) T cells expressed similarly elevated mRNA levels of CTLA 4 , GITR , Foxp 3 , CD 25 , and elevated protein levels of CTLA 4 ( p < 0 . 001 ) and GITR ( p < 0 . 001 ) . ^^^ However , only nave APB but not CB CD 4 ( + ) CD 25 ( + ) T cells showed suppression of allogeneic responses . ^^^ Stimulation of CD 4 ( + ) CD 25 ( ) T cells by MUTZ iDC ( MUTZ 3 specific immature dendritic cells ) elicited amplification of these genes and potent suppression ( 69 % + / 5 % and 71 % + / 3 % suppression , p < 0 . 001 , CB and APB , respectively ) on CD 4 ( + ) CD 25 ( ) T cell proliferation induced by MUTZ iDC but not by unrelated stimulators . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
RESULTS : We demonstrate that the tolerated allografts express high levels of FoxP 3 transcripts and contain a large number of CD 4 T cells , half of which express CD 25 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Islet antigen specific CD 4 ( + ) CD 25 ( ) T helper cells ( T ( H ) cells ) and T ( reg ) cells swarmed and arrested in the presence of autoantigens . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Telomere length of in vivo expanded CD 4 ( + ) CD 25 ( + ) regulatory T cells is preserved in cancer patients . ^^^ PURPOSE : CD 4 ( + ) CD 25 ( + ) regulatory T cells ( Treg ) are increased in the peripheral blood of cancer patients . ^^^ EXPERIMENTAL DESIGN : Treg and CD 4 ( + ) CD 25 ( ) T cells were isolated from peripheral blood of cancer patients ( n=23 ) and healthy age matched controls ( n=17 ) and analyzed for their content of T cell receptor excision circles ( TREC ) and for telomere length using flow FISH , real time PCR and Southern blotting . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In this article we show that DC elimination in vivo occurs in a perforin dependent manner and does not require IFN gamma or the presence of CD 4 ( + ) CD 25 ( + ) regulatory T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The identification of regulatory CD 4 ( + ) CD 25 ( + ) T cells has generated a great deal of renewed interest in cells that have immune regulatory properties . ^^^ This article will provide a brief historical review of suppressor T cells and cancer , experimental and clinical evidence that CD 4 ( + ) CD 25 ( + ) natural regulatory T cells play a role in cancer progression , and briefly discuss current strategies to inhibit these cells in an effort to enhance cancer immunotherapy . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Naturally occurring CD 25 ( + ) CD 4 ( + ) regulatory T cells ( T ( R ) cells ) , which specifically express the transcription factor Foxp 3 , engage in the maintenance of immunological self tolerance and suppressive control of aberrant or excessive immune responses to foreign antigens . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The immunohistology of tolerant grafts demonstrated the presence of CD 4 ( + ) CD 25 ( + ) T cells expressing Foxp 3 , and islet / kidney composite grafts from tolerant mice , but not from mice lacking lymphocytes , were accepted indefinitely when transplanted into nave B 6 mice , suggesting that recipient T cells were necessary to generate dominant tolerance . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Recent studies demonstrate that immunoregulatory dendritic cells ( iDCs ) confer immune hyporesponsiveness in part through CD 4 ( + ) CD 25 ( + ) T regulatory cells ( Tregs ) . ^^^ Herein , we provide evidence to support the hypothesis that dendritic cells derived from NOD mice and engineered ex vivo to exhibit suppressed expression of the CD 40 , CD 80 , and CD 86 costimulatory molecules motivate an increase in the prevalence of regulatory CD 4 ( + ) CD 25 ( + ) T cells via interleukin ( IL ) 7 . ^^^ Exogenous addition of IL 7 to NOD T cells did not promote expansion or proliferation , but instead selectively maintained the number of CD 4 ( + ) CD 25 ( + ) T cells by inhibiting activation of apoptosis in these cells . ^^^ In vitro , IL 7 receptor alpha chain ( IL 7Ralpha ) was expressed at significantly higher levels on CD 4 ( + ) CD 25 ( + ) T cells compared with CD 4 ( + ) CD 25 ( ) T cells irrespective of resting or stimulated state . ^^^ In vivo , CD 4 ( + ) CD 25 ( + ) T cells obtained from NOD scid mice reconstituted with ex vivo engineered iDCs and NOD splenocytes expressed significantly higher levels of IL 7Ralpha compared with levels in the CD 4 ( + ) CD 25 ( ) subset , especially in diabetes suppressive dendritic cell administered NOD scid recipients . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Our transfer experiments using total splenocytes and purified T cells demonstrated that the locus specifically controls the efficiency of disease protection mediated by the regulatory CD 4 ( + ) CD 25 ( + ) T cell subset . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The transcription factor FOXP 3 plays a key role in CD 4 ( + ) CD 25 ( + ) regulatory T cell function and represents a specific marker for these cells . ^^^ Despite its strong association with regulatory T cell function , in humans little is known about the frequency of CD 4 ( + ) CD 25 ( + ) cells that express FOXP 3 protein nor the distribution of these cells in vivo . ^^^ Flow cytometric analysis showed that FOXP 3 was expressed by the majority of CD 4 ( + ) CD 25 ( high ) T cells in peripheral blood . ^^^ By contrast , less than half of the CD 4 ( + ) CD 25 ( int ) population were FOXP 3 ( + ) , providing an explanation for observations in human T cells that regulatory activity is enriched within the CD 4 ( + ) CD 25 ( high ) pool . ^^^ Although FOXP 3 expression was primarily restricted to CD 4 ( + ) CD 25 ( + ) cells , it was induced following activation of both CD 4 ( + ) and CD 8 ( + ) T cell clones . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
A dysfunction in CD 4 ( + ) CD 25 ( + ) T regulatory cell specific transcription factor FoxP 3 leads to immune dysregulation , polyendocrinopathy , enteropathy 10 linked syndrome , often associated with atopic dermatitis . ^^^ RESULTS : Human T regulatory type 1 cells , their suppressive cytokines , IL 10 and TGF beta , as well as receptors for these cytokines were significantly expressed , whereas CD 4 ( + ) CD 25 ( + ) FoxP 3 ( + ) T regulatory cells were not found in lesional and atopy patch test atopic dermatitis or psoriasis skin . ^^^ CONCLUSION : A dysregulation of disease causing effector T cells is observed in atopic dermatitis lesions , in association with an impaired CD 4 ( + ) CD 25 ( + ) FoxP 3 ( + ) T cell infiltration , despite the expression of type 1 regulatory cells in the dermis . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Although multiple populations of cells appear to participate in this process , CD 4 ( + ) regulatory T cells that innately express CD 25 ( + ) appear to orchestrate the regulatory control of the immune response . ^^^ The recent identification that CD 4 ( + ) CD 25 ( + ) regulatory T cells preferentially express the Foxp 3 nuclear transcription factor and the development of molecular reagents to isolate antigen specific T cells have provided unique opportunities to explore immunoregulatory mechanisms after clinical marrow transplantation . ^^^ Recent studies in recipients of clinical bone marrow transplantation suggest that antigen specific CD 4 ( + ) CD 25 ( + ) Foxp 3 ( + ) T cells play a vital role in the regulatory control of GVH reactions mediated by both alloreactive and autoreactive lymphocytes . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) regulatory T cells ( Treg ) control the immune response to a variety of antigens , including self antigens . ^^^ Several models support the idea of the peripheral generation of CD 4 ( + ) CD 25 ( + ) Treg from CD 4 ( + ) CD 25 ( ) T cells . ^^^ Little is known about the endogenous factors and mechanisms controlling the peripheral expansion of CD 4 ( + ) CD 25 ( + ) Treg . ^^^ The administration of VIP to EAE mice results in the expansion of the CD 4 ( + ) CD 25 ( + ) , Foxp 3 expressing T cells in the periphery and the nervous system , which inhibit encephalitogenic T cell activation . ^^^ In addition to the increase in the number of CD 4 ( + ) CD 25 ( + ) Treg , VIP induces more efficient suppressors on a per cell basis . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In the present study , we have identified a subset of CD 4 ( + ) CD 25 ( + ) T cells with high levels of CTLA 4 and Foxp 3 ( intratumoral T ( reg ) cells ) that are overrepresented in biopsy specimens of B cell NHL ( median of 17 % in lymphoma biopsies , 12 % in inflammatory tonsil , and 6 % in tumor free lymph nodes ; P = . 001 ) . ^^^ We found that these CD 4 ( + ) CD 25 ( + ) T cells suppressed the proliferation and cytokine ( IFN gamma and IL 4 ) production of infiltrating CD 4 ( + ) CD 25 ( ) T cells in response to PHA stimulation . ^^^ PD 1 was found to be constitutively and exclusively expressed on a subset of infiltrating CD 4 ( + ) CD 25 ( ) T cells , and B 7 H1 could be induced on intratumoral CD 4 ( + ) CD 25 ( + ) T cells in B cell NHL . ^^^ Anti B 7 H1 antibody or PD 1 fusion protein partly restored the proliferation of infiltrating CD 4 ( + ) CD 25 ( ) T cells when cocultured with intratumoral T ( reg ) cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
A recently characterized subset of T cells , identified by their cell surface expression of CD 4 and CD 25 , is critical in regulating the function of other immune cells and preventing potentially harmful autoimmune responses . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
A role for regulatory T cells in cutaneous T Cell lymphoma ; induction of a CD 4 + CD 25 + Foxp3+ T cell phenotype associated with HTLV 1 infection . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
OBJECTIVE : Recent reports have shown that donor or host CD 4 ( + ) CD 25 ( + ) Treg cells can be used to control GVHD or graft rejection following allogeneic BMT in mice . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
However , we found that the two DC subtypes were equally potent at activating CD 4 T cells , based on up regulation of CD 69 and CD 25 expression . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Flow cytometric analysis of expression of transforming growth factor beta and glucocorticoid induced tumor necrosis factor receptor on CD 4 ( + ) CD 25 ( + ) T cells of patients with inflammatory bowel disease . ^^^ To determine whether human CD 4 ( + ) CD 25 ( + ) cells express glucocorticoid induced tumor necrosis factor receptor ( GITR ) and transforming growth factor beta ( TGF beta ) and the difference in CD 4 ( + ) CD 25 ( + ) cells between patients with inflammatory bowel diseases and healthy subjects , peripheral blood lymphocytes were obtained from patients with ulcerative colitis ( UC ; n = 50 ) , Crohn ' s disease ( CD ; n = 49 ) , and healthy volunteers ( control ; n = 50 ) and flow cytometric analysis was performed . ^^^ In control subjects , the expression of GITR on CD 4 ( + ) CD 25 ( + ) cells ( 41 . 8 + / 10 . 5 % ) was significantly higher than on CD 4 ( + ) CD 25 ( ) cells ( 11 . 1 + / 7 . 4 % ) . ^^^ Similarly , TGF beta expression on CD 4 ( + ) CD 25 ( + ) cells ( 5 . 3 + / 4 . 6 % ) was higher than on CD 4 ( + ) CD 25 ( ) cells ( 1 . 2 + / 1 . 4 % ) . ^^^ There were no significant differences among UC , CD , and control in CD 4 ( + ) CD 25 ( + ) / CD4 ( + ) ratio . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NF kappaB decoy ODN induced tolerance involved CD 4 ( + ) CD 25 ( + ) regulatory T cells ( Treg ) , because in vivo depletion of CD 25 ( + ) T cells abrogated the tolerance , whereas adoptive transfer of such T cell population from tolerant mice induced tolerance . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) regulatory T cells ( Tregs ) are critical in maintaining self tolerance and preventing organ specific autoimmunity . ^^^ Using flow cytometry to determine cell populations and real time polymerase chain reaction to assay mRNA expression for FOXP 3 , CTLA 4 , and GITR , we characterized CD 4 ( + ) CD 25 ( + ) T cells in paediatric SLE patients and healthy subjects . ^^^ The frequency of CD 4 ( + ) CD 25 ( + ) Tregs was significantly decreased in patients with active SLE compared with patients with inactive SLE and with controls ( 7 . 27 % + / 2 . 50 % , 9 . 59 % + / 2 . 80 % and 9 . 78 % + / 2 . 11 % , respectively ; P = 0 . 027 and P < 0 . 001 , respectively ) , and was inversely correlated with disease activity , as assessed with the Systemic Lupus Erythematosus Disease Activity Index 2000 scores ( r = 0 . 59 , P = 0 . 001 ) and serum anti double stranded DNA levels ( r = 0 . 65 , P < 0 . 001 ) . ^^^ Our preliminary investigations found elevated surface expression of GITR in CD 4 ( + ) CD 25 ( + ) T cells , elevated mRNA expression of CTLA 4 in CD 4 ( + ) T cells and higher amounts of mRNA expression for FOXP 3 in CD 4 ( + ) cells in patients with active SLE compared with patients with inactive disease and controls . ^^^ We demonstrated reduced CD 4 ( + ) CD 25 ( + ) Treg levels were inversely correlated with disease activity , indicating a defective Treg population in paediatric SLE patients . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
However , regulatory CD 4 ( + ) CD 25 ( + ) T cells which express Foxp 3 have also been shown to inhibit antitumor effector T cells . ^^^ Double or triple immunofluorescence staining was done to assess and quantify the activated CD 4 ( + ) CD 69 ( + ) T cells , regulatory CD 4 ( + ) Foxp 3 ( + ) T cells , and mixed CD 4 ( + ) CD 25 ( + ) T cells comprising both activated and regulatory T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In addition , CD 8 T cells can act to moderate this CD 4 T cell mediated pathology , possibly by increasing the ratio of putative CD25+ suppressor CD 4 T cells to CD 25 effector CD 4 T cells . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
This study was designed to evaluate the role ( s ) played by naturally occurring CD 4 ( + ) CD 25 ( + ) regulatory T cells in the development of Graves ' hyperthyroidism in resistant C57BL / 6 and susceptible BALB / c mice . ^^^ Depletion of CD 4 ( + ) CD 25 ( + ) T cells rendered some C57BL / 6 mice susceptible to induction of hyperthyroidism . ^^^ Thus , hyperthyroidism developed in 30 % of the CD 4 ( + ) CD 25 ( + ) T cell depleted C57BL / 6 mice immunized with adenovirus expressing the TSHR A subunit ( AdTSHR 289 ) vs . 0 % of those immunized with AdTSHR 289 alone . ^^^ This immunological manipulation also enhanced disease severity in susceptible BALB / c mice , as reflected by a significant increase in mean T ( 4 ) levels by CD 4 ( + ) CD 25 ( + ) T cell depletion . ^^^ The immunoenhancing effect of CD 4 ( + ) CD 25 ( + ) T cell depletion appears to be attributable to an increase in thyroid stimulating antibody production and / or a decrease in thyroid blocking antibody synthesis , but not immune deviation to either T helper 1 or 2 cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Differential distribution of CD 4 ( + ) CD 25 ( bright ) and CD 8 ( + ) CD 28 ( ) T cells in decidua and maternal blood during human pregnancy . ^^^ Recently , CD 4 ( + ) CD 25 ( + ) regulatory T cells have been identified in human decidua . ^^^ The aim of this study was to determine the distribution of specific T cell subsets in d . basalis and d . parietalis in early and term pregnancy , with a special emphasis on the presence of CD 4 ( + ) CD 25 ( bright ) ( regulatory ) T cells and CD 8 ( + ) CD 28 ( ) ( suppressor ) T cells . ^^^ We identified significantly higher percentages of CD 4 ( + ) CD 25 ( bright ) and CD 8 ( + ) CD 28 ( ) T cells in decidua compared to peripheral blood suggesting an important role for these T cell subsets locally at the fetal maternal interface . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The failure of the immune response may be naturally programmed and related to a specific subpopulation of CD 4 ( + ) CD 25 ( + ) regulatory T cells , whose function is to protect us against autoimmunity . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Our data demonstrate that both the anti CD 25 IT and the anti CD 25 MACS were equally effective in depleting CD 4 ( + ) CD 25 ( + ) cells and in sparing T cells that were reactive with third party cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
However , recent studies have revealed that a subset of CD 4 ( + ) T cells , referred to as CD 4 ( + ) CD 25 ( + ) regulatory T cells ( Treg ) , may accumulate in the tumor environment and suppress tumor specific T cell responses , thereby hindering tumor rejection . ^^^ The presence of infiltrating CD 4 ( + ) CD 25 ( + ) Treg may be detrimental to the host defense against the tumor , while the presence of effector T lymphocytes , including CD 8 ( + ) T cells and non regulatory CD 4 ( + ) helper T cells may be beneficial . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In this study , the frequency of Tregs in liver transplant recipients was determined based on flow cytometric analysis of CD 4 , CD 25 , CD45RO , and cytotoxic T lymphocyte antigen ( CTLA ) 4 markers , and the suppressor activity of Tregs was assessed in a mixed leukocyte reaction . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
One discovery of particular importance was the identification of naturally occurring CD 4 ( + ) CD 25 ( + ) Treg that arise in the thymus and express the transcription factor Foxp 3 ; however , Treg can also be induced in the periphery after immune activation . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
When APCTCM were activated by a maturation cocktail , CD 83 , CD 86 , HLA DR and CD 25 were up regulated to levels considerably higher than in DCIL 10 and comparable to DCims while CD 14 , CD 16 , CD 4 and dextran uptake were down modulated . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Anti CD45RB treated mice exposed to alloantigen exhibited anergic CD 4 ( + ) CD 25 ( ) effector cells and regulatory CD 4 ( + ) CD 25 ( + ) cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We show in this study that long term tolerance to allogeneic skin grafts can be established in the absence of immunosuppression by the combination of the following elements : 1 ) augmenting the frequency of regulatory CD 4 ( + ) CD 25 ( + ) T cells ( Treg ) and 2 ) presentation of the allogeneic stimuli through linked recognition of allo and self epitopes on semiallogeneic F ( 1 ) APCs . ^^^ BALB / c spleen cells enriched for CD 4 ( + ) CD 25 ( + ) T lymphocytes were transferred either to BALB / c nu / nu mice or to BALB / c nu / nu previously injected with F ( 1 ) ( BALB / c 10 B6 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Among the various forms of regulatory T cells described , CD 4 ( + ) CD 25 ( + ) T cells have emerged as one of the most potent tolerogenic subsets . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Here , we show that naturally arising CD 4 ( + ) CD 25 ( + ) regulatory T cells , which actively maintain immunological tolerance to self and nonself antigens , are powerful inhibitors of atherosclerosis in several mouse models . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Recent studies have explored the role of ' natural ' CD 4 ( + ) CD 25 ( + ) regulatory T cells ( Tregs ) in the suppression of tumor immunity in cancer patients . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Although these defects were T cell intrinsic , we also observed abundant CD 4 ( + ) CD 25 ( + ) FoxP 3 ( + ) T cells , as well as transcripts for FoxP 3 , IL 10 , PD L1 / B7 H 1 , and indoleamine 2 , 3 dioxygenase ( IDO ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Compared with the control mice , newborn mice treated with monoclonal antibody ( MoAb ) against mouse CD 25 ( + ) CD 4 ( + ) T cells together with Reo 2 and CpG ODN greatly reduced the absolute number of splenic CD 25 ( + ) T cells and resulted in the development of severe insulitis , leading to an overt early diabetes . ^^^ These evidences suggest that CD 4 ( + ) CD 25 ( + ) T cell may , at least in part , maintain tolerance to Reo 2 triggered and CpG ODN induced prolonged mild Th 1 dependent autoimmune insulitis , leading to the overt disease . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Moreover , in EFNB 1 treated fetal thymus organ culture , CD 117 ( + ) , CD 25 ( + ) , DP , CD 4 SP , and CD 8 SP cells all had significantly enhanced proliferation history , according to bromodeoxyuridine uptake . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Flowcytometric analysis was performed after staining for surface CD 4 , CD 8 , and CD 25 and for intracellular perforin , granzyme B , active caspase 3 , and TGF beta . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In mixed lymphocyte reaction ( MLR ) cultures , IFN lambda treated DCs specifically induced IL 2 dependent proliferation of a CD 4 ( + ) CD 25 ( + ) Foxp 3 ( + ) T cell subset with contact dependent suppressive activity on T cell proliferation initiated by fully mature DCs . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) T cells have been implicated in HCV persistence because their frequency is increased in the blood of HCV infected patients and their in vitro depletion results in increased IFN gamma production by HCV specific T cells . ^^^ Studying a well characterized cohort of 16 chimpanzees , the sole animal model for HCV infection , we here demonstrate that the frequency of Foxp 3 ( + ) CD 4 ( + ) CD 25 ( + ) regulatory T cells ( T ( Regs ) ) and the extent of suppression was as high in spontaneously HCV recovered chimpanzees as in persistently HCV infected chimpanzees . ^^^ Foxp 3 ( + ) CD 4 ( + ) CD 25 ( + ) T ( Regs ) suppressed IFN gamma production , expansion , and activation induced cell death of HCV specific T cells after recovery from HCV infection and in persistent HCV infection . ^^^ However , Foxp 3 ( + ) CD 4 ( + ) CD 25 ( + ) T ( Reg ) cells of both HCV recovered and HCV infected chimpanzees differed from Foxp 3 ( + ) CD 4 ( + ) CD 25 ( + ) T ( Reg ) cells of HCV naive chimpanzees in increased IL 2 responsiveness and lower T cell receptor excision circle content , implying a history of in vivo proliferation . ^^^ This result suggests that HCV infection alters the population of Foxp 3 ( + ) CD 4 ( + ) CD 25 ( + ) T ( Reg ) cells . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
A prospective randomized controlled trial was designed to observe expressions of T lymphocyte subsets ( CD 3 ( + ) , CD 3 ( + ) CD 4 ( + ) and CD 3 ( + ) CD 8 ( + ) ) , natural killer cells ( CD 3 ( ) CD 16 ( + ) CD 56 ( + ) ) , and activated T lymphocytes ( CD 3 ( + ) CD 25 ( + ) ) of patients undergoing gastric cancer surgeries and receiving patient controlled intravenous analgesia ( PCIA ) . ^^^ Expressions of CD 3 ( + ) , CD 3 ( + ) CD 4 ( + ) , CD 3 ( + ) CD 8 ( + ) , CD 3 ( ) CD 16 ( + ) CD 56 ( + ) , and CD 3 ( + ) CD 25 ( + ) were measured as percentages of total lymphocytes by flow cytometer at 5 time points . ^^^ RESULTS : There was no significant difference in analgesic efficacy and the baselines of CD 3 ( + ) , CD 3 ( + ) CD 4 ( + ) , CD 3 ( + ) CD 8 ( + ) , CD 3 ( ) CD 16 ( + ) CD 56 ( + ) , and CD 3 ( + ) CD 25 ( + ) in all groups . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Thymic repertoire selection also induces the development of CD 25 ( + ) CD 4 ( + ) regulatory T cells , which play important roles for maintaining peripheral tolerance . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) regulatory T cells play an important role in preventing autoimmunity . ^^^ We investigated the presence of CD 4 ( + ) CD 25 ( + ) regulatory T cells in the peripheral blood of patients with rheumatoid arthritis ( RA ) , systemic lupus erythematosus ( SLE ) , and systemic sclerosis ( SSc ) , using flow cytometry . ^^^ The percentage of CD 4 ( + ) CD 25 ( + ) regulatory T cells was significantly decreased in RA , especially in patients with high serum levels of either CRP or MMP 3 . ^^^ In SSc and SLE , the percentage of CD 4 ( + ) CD 25 ( + ) regulatory T cells was higher in patients than in controls , but not significant . ^^^ We also investigated the serum levels of IL 10 , which influences the function of CD 4 ( + ) CD 25 ( + ) regulatory T cells and other regulatory T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Few CD 4 ( + ) and CD 8 ( + ) T cells expressed an activated phenotype ( CD 44 ( hi , ) CD 25 ( + ) , CD 69 ( + ) , CD62L ( lo ) , CD45RB ( lo ) ) at the sites of clonal expansion ( lymph nodes , spleen , and blood ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In this study , we show that naive wild type or CD 28 ( / ) CD 4 ( + ) CD 25 ( ) T cells exposed to peptide in vivo develop regulatory activity that suppresses the response of adoptively transferred naive T cells to a subsequent immunogenic challenge . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Burn injury induced higher cell surface CD 25 and CD 152 expression on lymph node CD 4 T cells , whereas SEB treatment increased CD 25 and CD 69 expression on CD 4 and CD 8 T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) T regulatory cells ( T ( reg ) ) can inhibit the proliferation and cytokine secretion of CD 4 ( + ) CD 25 ( ) helper T cells in mice and humans . ^^^ In vitro incubation of human PBMCs with LMB 2 reduced the levels of CD 4 ( + ) CD 25 ( + ) and Foxp 3 expressing cells without impairing the function of the remaining lymphocytes . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) FOXP 3 ( + ) regulatory T cells ( CD 4 ( + ) Treg cells ) are thought to differentiate in the thymus and immigrate from the thymus to the periphery . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We show here that absolute counts of both CD 4 ( + ) T cells and CD 4 ( + ) CD 25 ( + ) FOXP 3 ( + ) CD45RO ( + ) T cells ( T ( regs ) ) are greatly diminished in patients with malignant glioma , but T ( regs ) frequently represent an increased fraction of the remaining CD 4 compartment . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
This was achieved through immune deviation to a T helper cell response with increased IL 10 and TGF beta production and activation of regulatory CD 4 ( + ) CD 25 ( + ) T cells . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
RESULTS : Exposure to MF for 2 h and subsequent in vitro stimulation in the presence of the appropriate mitogen , caused a decrease of interferon gamma production , a decrease of cell proliferation , a decrease of expression of CD 25 and a decrease of cytosolic free calcium concentration in exposed CD 4 ( + ) T cell lines . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) regulatory T cell lines of both patterns expressed CD 25 and reacted with activated autologous T cells but not resting T cells , irrespective of antigen specificity of the target T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) T regulatory ( Treg ) cells are a CD 4 ( + ) T cell subset involved in the control of the immune response . ^^^ In vitro , murine CD 4 ( + ) CD 25 ( + ) Treg cells inhibit CD 4 ( + ) CD 25 ( ) Th cell proliferation induced by anti CD 3 mAb in the presence of APCs . ^^^ The addition of IL 4 to cocultured cells inhibits CD 4 ( + ) CD 25 ( + ) Treg cell mediated suppression . ^^^ Since all cell types used in the coculture express the IL 4Ralpha chain , we used different combinations of CD 4 ( + ) CD 25 ( ) Th cells , CD 4 ( + ) CD 25 ( + ) Treg cells , and APCs from wild type IL 4Ralpha ( + / + ) or knockout IL 4Ralpha ( / ) mice . ^^^ Results show that the engagement of the IL 4Ralpha chain on CD 4 ( + ) CD 25 ( ) Th cells renders these cells resistant to suppression . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
LKC was exacerbated in euthymic mice depleted of CD 4 ( + ) CD 25 ( + ) forkhead / winged helix transcription factor ( + ) regulatory T cells . ^^^ The results suggest that DS exposes shared epitopes in the cornea , conjunctiva , and LG that induce pathogenic CD 4 ( + ) T cells that produce LKC , which under normal circumstances is restrained by CD 4 ( + ) CD 25 ( + ) forkhead / winged helix transcription factor ( + ) regulatory T cells . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Surface expression of both CD 25 and CD 69 was elevated on freshly isolated splenic CD 4 ( + ) T cells from SHIP KO and Dok 1 / SHIP DKO , suggesting these cells were constitutively activated . ^^^ Interestingly , the CD 4 ( + ) T cells from SHIP KO and Dok 1 / SHIP DKO mice produced higher levels of TGF beta , expressed Foxp 3 , and inhibited IL 2 production by CD 3 stimulated CD 4 ( + ) CD 25 ( ) T cells in vitro . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Furthermore , ephrin A 1 costimulation suppresses up regulation of both the IL 2R alpha chain ( CD 25 ) and early activation Ag CD 69 and can block IL 2 production by CD 4 ( + ) single positive cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
After ALS treatment , CD 4 ( + ) CD 25 ( + ) T cells underwent proliferation and expressed a memory T cell marker , CD 44 . ^^^ By real time PCR analyses , ALS treatment of CD 4 deficient mice adoptively transferred with Thy1 . 2 ( + ) CD 4 ( + ) CD 25 ( + ) Foxp 3 ( + ) and Thy1 . 1 ( + ) CD 4 ( + ) CD 25 ( ) Foxp 3 ( ) T cells resulted in the appearance of Thy1 . 2 ( + ) CD 4 ( + ) CD 25 ( ) Foxp 3 ( + ) and Thy1 . 1 ( + ) CD 4 ( + ) CD 25 ( + ) Foxp 3 ( + ) T cells , suggesting the conversion between CD 25 ( + ) and CD 25 ( ) T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Regulatory CD 4 ( + ) CD 25 ( + ) T cells ( Tregs ) are defective numerically and functionally in autoimmune hepatitis ( AIH ) . ^^^ Direct contact is necessary for Tregs to suppress proliferation and IFN gamma production by CD 4 ( + ) CD 25 ( ) and CD 8 ( + ) T cells in patients and controls . ^^^ Both in patients and controls , FOXP 3 expression is present also in CD 4 ( + ) CD 25 ( ) T cells , although at a low level and not associated to suppressive function . ^^^ Both in patients and controls , addition of Tregs does not influence target cell apoptosis , but in AIH , spontaneous apoptosis of CD 4 ( + ) CD 25 ( ) T cells is reduced . ^^^ Deficient CD 4 ( + ) CD 25 ( ) T cell spontaneous apoptosis may contribute to the development of autoimmunity . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) high regulatory T ( Treg ) cells are pivotal for the maintenance of self tolerance of pathogenic alloresponses after solid organ or bone marrow transplantation in murine model systems . ^^^ To investigate the expression of HLA G on the surface of peripheral mononuclear ( PMNL ) cells , we have used monoclonal antibodies in flow cytometry to estimate CD 4 , CD 25 , CD 45 , and HLA G content . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Flow cytometric analysis of NWC after the primary treatment showed marked increases in the CD 4 ( ) CD 8 ( ) non T cell population bearing CD 25 ( + ) , CD45RB ( super high ) and CD 122 ( + ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Flow cytometry showed that the cells were CD 4 ( ) and CD 8 ( ) , but CD 44 ( + ) , CD 25 ( + ) and cytoplasmic CD 3 ( + ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
IL 15 upregulated CD45RO and CD 25 whereas it down regulated CD62L expression of CD3 / CD28 stimulated CD 4 ( + ) T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Naturally occurring CD 4 ( + ) CD 25 ( + ) T regulatory ( Treg ) cells have been shown to inhibit adaptive responses by T cells . ^^^ We investigated whether CD 4 ( + ) CD 25 ( + ) Treg cells could affect NK cell function in vivo by using allogeneic ( full H 2 disparate ) bone marrow ( BM ) transplantation and the model of hybrid resistance , in which parental marrow grafts are rejected solely by the NK cells of irradiated ( BALB / c 10 C57BL / 6 ) F ( 1 ) recipients . ^^^ The inhibitory role of Treg cells on NK cells was confirmed in vivo with adoptive transfer studies in which transferred CD 4 ( + ) CD 25 ( + ) cells could abrogate NK cell mediated hybrid resistance . ^^^ Thus , CD 4 ( + ) CD 25 ( + ) Treg cells can potently inhibit NK cell function in vivo , and their depletion may have therapeutic ramifications for NK cell function in BM transplantation and cancer therapy . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Accumulating evidence indicates an immunosuppressive role for CD 4 ( + ) CD 25 ( + ) regulatory T cells ( Tregs ) in autoimmune diseases . ^^^ In the present study , the phenotypic and functional characteristics of CD 4 ( + ) CD 25 ( + ) T cells isolated from the peripheral blood of patients with RR MS and secondary progressive multiple sclerosis ( SP MS ) were investigated . ^^^ No significant quantitative or phenotypic abnormalities in CD 4 ( + ) CD 25 ( + ) T cells from RR and SP MS patients were detected . ^^^ However , whereas a reduced suppressor function of CD 4 ( + ) CD 25 ( + ) T cells toward proliferation and interferon gamma production of CD 4 ( + ) CD 25 ( ) responder T cells was found in RR MS patients , SP MS patients showed a normal Treg function . ^^^ The suppressive capacity of MS derived CD 4 ( + ) CD 25 ( + ) T cells was correlated with disease duration but not with age , indicating that Treg function is more affected in the early phase of the disease process . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Because there is significant expression of MHC class 2 ( MHC 2 ) determinants ( DR ) on a subpopulation CD4+ CD 25 ( high ) regulatory T cells ( Treg ) , we examined the function of CD 4 cells expressing MHC DR . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
RESULTS : CD4+ CD 25 ( high ) lymphocytes from controls expressed low levels of CD 69 , CD 154 or CD 30 , but also expressed glucocorticoid induced tumour necrosis factor receptor , high levels of intracellular cytotoxin T lymphocyte associated antigen 4 , CD45RO and diminished amounts of CD 4 , all of which are phenotypic characteristics of natural regulatory T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
PURPOSE : We evaluated the effects of celecoxib treatment on tumor infiltrating lymphocyte ( TIL ) subsets [ CD 3 ( + ) , CD 4 ( + ) , CD 8 ( + ) , CD 25 ( + ) , and T cell receptor ( TCR ) zeta expressing cells ] and tryptase positive mast cells in cervical tumors . ^^^ There was no significant treatment related difference in the percentage of CD 3 ( + ) , CD 4 ( + ) , CD 8 ( + ) , and CD 25 ( + ) TIL as well as in tryptase positive cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We showed that dendritic cell precursors ( DCp ) cultured with anti CD200R2 / 3 develop the capacity to induce CD 4 ( + ) CD 25 ( + ) regulatory T cells ( Treg ) from peripheral lymphocytes . ^^^ RESULTS : Foxp 3 ( + ) CD 4 ( + ) CD 25 ( + ) Treg were derived from 60 hr thymocyte and splenocyte T cell cultures using both DC populations . ^^^ CONCLUSIONS : We conclude that CD 200 triggering of bone marrow DCs in the absence of CD200R1 engagement induces CD 4 ( + ) CD 25 ( + ) Treg , and these cloned antigen specific Treg may have clinical utility . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Subclasses of T , B and NK lymphocytes were tested for in the blood samples : CD 3 , CD 4 , CD 5 , CD 7 , CD 8 , CD11B , CD 16 , CD 20 , CD 25 , CD 38 , CD45RA , CD 50 , CD 71 and CD 95 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The numbers of cells expressing CD 2 , CD 4 , CD 8 , and CD 172 ( the 74 22 15 ( SWC 3 ) antibody is now known to be specific for CD 172 ) , MHC class 2 , and CD 25 were quantified using immunohistochemistry . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In addition , immunological analysis revealed that the DNA vaccine induced CD 4 ( + ) CD 25 ( + ) T cells cultured from draining lymph nodes that had immunosuppressive function in vitro . ^^^ Data also revealed that CD 4 ( + ) CD 25 ( ) T cells from mice immunized with the DNA vaccine yielded a cell population that was foxp 3 ( + ) , showed increased expression of CD 25 compared to control , and had immunosuppressive function in vitro , indicating that Tregs could have developed from antigen induced , peripheral T lymphocytes . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We conclude that lymphoma cells may be eradicated using DC vaccination if activation of CD 25 ( + ) Tregs is simultaneously inhibited , and that intentionally enhanced endosomal antigen processing in DC vaccines may shift the balance from CD 4 T cell help toward stimulation of Tregs . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In addition , regulatory CD 4 ( + ) CD 25 ( + ) T cells ( T ( REG ) ) were confirmed to play a critical role in the induction of hyporesponsiveness by anti CD40L and anti CD 86 mAb . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We demonstrate that during immunity to a tumor / self Ag , the predominant role of Th cell derived IL 2 was to maintain IL 2Ralpha ( CD 25 ) on CD 4 ( + ) T regulatory cells ( T ( reg ) ) , which resulted in their maintenance of the T ( reg ) cell lineage factor , Forkhead / winged helix transcription factor ( Foxp 3 ) , and tolerance . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Ag specific hyporesponsiveness could be reversed by the addition of high dose IL 2 but not by removal of CD 4 ( + ) CD 25 ( + ) T cells , indicating that hyporesponsiveness was due to anergy and not due to active suppression . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In this study , we investigate the relationship between peptide induced , CD 4 ( + ) regulatory T cells and naturally occurring CD 4 ( + ) CD 25 ( + ) cells derived from the Tg 4 TCR transgenic mouse . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Furthermore , we showed that the percentages of CD 4 ( + ) cells , CD 8 ( + ) cells , and CD 4 ( + ) CD 25 ( + ) cells in the splenocytes of mice fed with CS are significantly higher than those of the control . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
A low CD 4 ( + ) FOXP 3 ( + ) T cell count early after SCT ( day 30 ) was associated with an increased risk of GVHD , and the ratio of CD 4 ( + ) FOXP 3 ( + ) T cells to CD 4 ( + ) CD 25 ( + ) FOXP 3 ( ) T cells was significantly reduced in patients with GVHD , suggesting diminished control of effector T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Furthermore , leukocyte subpopulations , and surface markers of lymphocytes and monocytes ( CD 3 ; CD 4 ; CD45RO ; CD4 / CD45RO ; CD 25 ; CD 4 and CD 25 ; CD 54 ; CD 69 ; CD14 / HLA DR ; CD 8 ; CD3 / HLA DR ; CD 4 : CD 8 ratio ) were assessed . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Flow cytometric analaysis of lymphocyte supopulations documented increased counts of CD 4 and CD 8 cells and an inreased activation marker expression on lymphocytes ( CD 25 ) and monocytes ( MAC 3 ) in neem treated mice compared to the control animals . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In healthy rodents and humans , CD 4 ( + ) T cells constitutively expressing the interleukin ( IL ) 2 receptor alpha chain ( CD 25 ) are able to exert such suppressive function in vitro and in vivo . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Based on the enhanced infiltration of tumors by CD 4 ( + ) CD 25 ( + ) TIL in Flt 3 ligand + GM CSF treated mice , this could reflect the dominant influence of regulatory T cells in situ . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We sought to improve on this strategy by combining xenogeneic DNA vaccination with an agonist anti glucocorticoid induced tumor necrosis factor receptor family related gene ( GITR ) monoclonal antibody ( mAb ) , DTA 1 , which has been shown previously both to costimulate activated effector CD 4 ( + ) and CD 8 ( + ) T cells and to inhibit the suppressive activity of CD 4 ( + ) CD 25 ( + ) regulatory T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The Foxp 3 protein is a specific marker of CD 4 ( + ) CD 25 ( + ) regulatory T ( T ( reg ) ) cells , and its expression is critical to their development and function . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Finally , naturally occurring CD 4 ( + ) 25 ( + ) T cells seem to have the same effect on PKCtheta recruitment in CD 25 ( ) T cells of the same Ag specificity . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Reconstitution of T cell deficient mice by an adoptive transfer of mature peripheral lymphocytes was accompanied by the development of a CD 25 ( + ) Foxp 3 ( + ) CTLA 4 ( + ) CD 4 ( + ) regulatory T cell population that acted to dampen Ag driven cell cycle progression and facilitate the induction of clonal anergy in nearby responder CD 25 ( ) CD 4 ( + ) T cells . ^^^ Thus , an early recovery of CD 25 ( + ) regulatory T cells following a lymphopenic event can prevent exuberant Ag stimulated CD 4 ( + ) cell cycle progression and promote the development of clonal anergy . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We previously showed that T cell development was inhibited beginning at the CD 4 ( ) CD 8 ( ) CD 25 ( + ) CD 44 ( low ) stage in ADA deficient FTOC initiated at day 15 of gestation when essentially all thymocytes are CD 4 ( ) CD 8 ( ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Estrogen ( E 2 ) induced immunomodulation involves dual effects on antigen presenting cells ( APC ) and CD 4 ( + ) CD 25 ( + ) regulatory T cells ( Treg ) but not a direct effect on effector T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
For immunohistochemical stainings anti CD 3 , anti CD 19 , anti CD 4 , anti CD 8 , anti CD 25 , anti CD 28 , anti CTLA 4 ( CD 152 ) , anti bcl 2 , anti Fas , and anti FasL antibodies were used . ^^^ RESULTS : The expression of CD 4 , CD 25 , CD 28 , FasL , and CTLA 4 was significantly increased in group 1 compared to group 2 ( p < 0 . 05 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Increase of CD 4 ( + ) CD 25 ( + ) T cells in Smad 3 ( / ) mice . ^^^ AIM : To investigate the changes of lymphocyte subpopulations , especially CD 4 ( + ) CD 25 ( + ) T regulatory cells in Smad 3 ( / ) mice . ^^^ More important , Smad 3 ( / ) mice had an increased population of CD 4 ( + ) CD 25 ( + ) T cells in peripheral lymphoid tissues , including thymus , spleen , and lymph nodes . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Here , we report the identification of the ubiquitin like gene UBD as a downstream element of FOXP 3 in human activated regulatory CD 4 ( + ) CD 25 ( hi ) T cells ( T ( reg ) ) . ^^^ Retroviral transduction of UBD in human allo reactive effector CD 4 ( + ) T helper ( T ( h ) ) cells upregulates CD 25 and mediates downregulation of IL 4 and IL 5 expression similar to overexpression of FOXP 3 . ^^^ However , high levels of LGALS 3 protein expression were observed only in human CD 4 ( + ) CD 25 ( hi ) derived T ( reg ) cells and FOXP 3 transduced T ( h ) cells , whereas little was induced in UBD transduced T ( h ) cells . ^^^ High levels of LGALS 3 expression represent a FOXP 3 signature of human antigen stimulated CD 4 ( + ) CD 25 ( hi ) derived regulatory T cells . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In this commentary , PI Tregs are considered in comparison to other Tregs , such as the natural CD 4 ( + ) CD 25 ( + ) and Tr 1 cells , and there seems to be considerable overlap in gene expression between all three Treg populations , but also with both Th 1 and Th 2 cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Thymus derived regulatory T cells ( Tregs ) expressing CD 4 , CD 25 , and the transcription factor Foxp 3 play major roles in preventing autoimmunity . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Autoimmune regulatory cells were CD 4 ( + ) CD 25 ( + ) , but not CD 4 ( + ) CD 25 ( ) , T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Hypereosinophilia in a patient with invasive thymoma with clonal T lymphocyte expansion expressing CD 4 , CD 8 , and CD 25 antigens . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Here we show that mice deficient in NF kappaB inducing kinase have a complex phenotype consisting of immunosuppression mediated by CD 25 ( ) Foxp 3 ( ) memory CD 4 ( + ) cells and , in the absence of those cells , hyper responsive naive CD 4 ( + ) T cells , which caused autoimmune lesions after adoptive transfer into hosts deficient in recombination activating genes . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In addition , we showed that a chimeric anti CCR 4 monoclonal antibody ( mAb ) could deplete CCR 4 ( + ) T cells and inhibit the migration of CD 4 ( + ) CD 25 ( + ) T cells in vitro . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Because usual myeloablation and / or immunosuppression are risk factors in most pathologies , we assessed the potential of monospecific CD 4 ( + ) CD 25 ( + ) regulatory T cells ( Tregs ) to engraft minor mismatched BM without preconditioning . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Lymphocyte subpopulations were studied by flow cytometry , including the markers : CD 3 , CD 4 , CD 7 , CD 8 , CD45RO , CD 38 , HLA DR and CD 25 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
An addition of anti OX 40 ligand ( OX40L ) mAb suppressed CD 25 expression , proliferation and IFN gamma production of CD 8 ( + ) T cells , suggesting that OX 40 functions as a costimulatory molecule not only for CD 4 ( + ) T cells but also for CD 8 ( + ) T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Depletion of CD 4 ( + ) CD 25 ( + ) regulatory T cells exacerbated the disease , whereas HA specific CD 4 ( + ) CD 25 ( + ) T cells given i . v . controlled the disease . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
IL 1beta drives proliferation and cytokine production by CD 4 ( + ) CD 25 ( + ) FoxP 3 ( ) effector / memory T cells , attenuates CD 4 ( + ) CD 25 ( + ) FoxP 3 ( + ) regulatory T cell function , and allows escape of CD 4 ( + ) CD 25 ( ) autoreactive effectors from suppression . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Analysis of tumor infiltrating T lymphocyte populations showed that the expression of Foxp 3 and IL 10 was associated with CD 4 ( + ) CD 25 ( + ) T cells . ^^^ Repeated treatment resulted in a progressive increase in tumor infiltrating CD 4 ( + ) CD 25 ( + ) Foxp 3 ( + ) T suppressor cells establishing their role in long term neutralization of antitumor activity . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Splenic CD 3 ( + ) , CD 4 ( + ) , CD 3 ( + ) CD 25 ( + ) , and CD 4 ( + ) CD 44 ( + ) counts were significantly increased in mice after lymphocyte transfer from IRI mice vs . mice with lymphocytes from sham mice . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) FOXP 3 ( + ) regulatory T cells ( Treg ' s ) play an important role in the maintenance of immune tolerance . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Preparation of CD 25 ( + ) and CD 25 ( ) CD 4 ( + ) T cells for glycomic analysis a cautionary tale of serum glycoprotein sequestration . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Naturally occurring CD 25 ( + ) CD 4 ( + ) regulatory T cells ( Tregs ) actively engage in the maintenance of immunologic self tolerance and immunoregulation . ^^^ Although several cell surface molecules have been reported as Treg specific markers , such as CD 25 , glucocorticoid induced TNFR family related gene / protein and CTL associated molecule 4 , they are also expressed on activated T cells derived from CD 25 ( ) CD 4 ( + ) naive T cells . ^^^ To identify Treg specific molecules controlled by Foxp 3 , we performed DNA microarray analysis by comparing the following pairs of cell populations : fresh CD 25 ( + ) CD 4 ( + ) T cells versus fresh CD 25 ( ) CD 4 ( + ) T cells , activated CD 25 ( + ) CD 4 ( + ) T cells versus activated CD 25 ( ) CD 4 ( + ) T cells and retrovirally Foxp 3 transduced CD 25 ( ) CD 4 ( + ) T cells versus mock transduced CD 25 ( ) CD 4 ( + ) T cells . ^^^ Retroviral transduction of either group of genes in CD 25 ( ) CD 4 ( + ) T cells failed to confer in vitro suppressive activity . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We performed lesional skin biopsies before and after treatment , that were stained immunohistochemically with monoclonal antibodies to CD 4 , CD 25 , forkhead / winged helix transcription factor ( FoxP 3 ) , interleukin ( IL ) 10 and transforming growth factor ( TGF ) beta . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Characteristics of rat CD 4 ( + ) CD 25 ( + ) T cells and their ability to prevent not only diabetes but also insulitis in an adoptive transfer model in BB rats . ^^^ Human and mouse CD 4 ( + ) CD 25 ( + ) T cells have been intensively studied through the last decade . ^^^ This study describes the phenotype of rat CD 4 ( + ) CD 25 ( + ) Foxp 3 ( + ) T cells and the site in which they exert regulation in a transfer induced autoimmune diabetes model . ^^^ Several proteins and mRNAs are up regulated in unstimulated rat CD 4 ( + ) CD 25 ( + ) T cells compared with CD 4 ( + ) CD 25 ( ) T cells , including Foxp 3 , Lag 3 , CD 80 , interleukin 10 ( IL 10 ) and CTLA 4 . ^^^ To investigate CD 4 ( + ) CD 25 ( + ) T cells in vivo , we transferred three million diabetogenic T cells either alone or in combination with two million CD 4 ( + ) CD 25 ( + ) T cells to 30 day old BB rats . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Cre mediated thymocyte specific ablation of c Myc in CD 25 ( + ) CD 44 ( ) thymocytes reduced proliferation and cell growth at the pre TCR checkpoint , resulting in thymic hypocellularity and a severe reduction in CD 4 ( + ) CD 8 ( + ) thymocytes . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Adoptive transfer experiments revealed that DNA vaccination generated protective CD 4 ( + ) regulatory T cells ( Tr ) of either CD 25 ( + ) or CD 25 ( ) phenotype . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
IL 10 production by CD 25 ( ) FoxP 3 ( ) IL 10 ( + ) CD 4 ( + ) T cells is a strong correlate of disease progression , and BMDC from wild type mice inhibited expansion of these cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In addition , their functional activity resembled that of Treg , as evidenced by a poor proliferative capacity ; suppression of proliferation of CD 25 CD 4 or CD8T cells and inhibition of interferon gamma release by CD 25 CD4+ T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
To modulate the immune inhibitory microenvironment in tumors , naturally occurring CD4+ CD25+ Treg cells were depleted by cytotoxic CD 4 or CD 25 antibodies . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) regulatory cells play a crucial role in controlling various autoimmune diseases , and a deficiency in their number or function could be involved in disease development . ^^^ The current study shows that NOD mice had fewer CD 4 ( + ) CD 25 ( + ) regulatory cells , which expressed normal levels of glucocorticoid induced tumor necrosis factor receptor and cytotoxic T lymphocyte associated antigen 4 . ^^^ We have also found that NOD CD 4 ( + ) CD 25 ( + ) cells regulate poorly in vitro after stimulation with anti CD 3 and NOD APCs in comparison with B 6 CD4 ( + ) CD 25 ( + ) cells stimulated with B 6 APCs . ^^^ Surprisingly , stimulation of NOD CD 4 ( + ) CD 25 ( + ) cells with B 6 APCs restored regulation , whereas with the reciprocal combination , NOD APCs failed to activate B 6 CD4 ( + ) CD 25 ( + ) cells properly . ^^^ Interestingly , APCs from disease free ( > 30 weeks of age ) , but not diabetic , NOD mice were able to activate CD 4 ( + ) CD 25 ( + ) regulatory function in vitro and apparently in vivo because only spleens of disease free NOD mice contained potent CD 4 ( + ) CD 25 ( + ) regulatory cells that prevented disease development when transferred into young NOD recipients . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Abnormalities in CD 4 ( + ) CD 25 ( + ) Foxp 3 ( + ) regulatory T ( T reg ) cells have been implicated in susceptibility to allergic , autoimmune , and immunoinflammatory conditions . ^^^ In healthy volunteers , peripheral blood CD 25 ( + ) CD 127 ( lo ) cells comprised 6 . 35 + / 0 . 26 % of CD 4 ( + ) T cells , of which 2 . 05 + / 0 . 14 % expressed the naive subset marker CD45RA . ^^^ Expression of FoxP 3 protein and the CD 127 ( lo ) phenotype were highly correlated within the CD 4 ( + ) CD 25 ( + ) population . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Most T reg cells are defined based on expression of CD 4 , CD 25 , and the transcription factor , FoxP 3 . ^^^ A combination of CD 4 , CD 25 , and CD 127 resulted in a highly purified population of T reg cells accounting for significantly more cells that previously identified based on other cell surface markers . ^^^ In fact , cells separated based solely on CD 4 and CD 127 expression were anergic and , although representing at least three times the number of cells ( including both CD 25 ( + ) CD 4 ( + ) and CD 25 ( ) CD 4 ( + ) T cell subsets ) , were as suppressive as the `` classic ' ' CD 4 ( + ) CD 25 ( hi ) T reg cell subset . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The present study demonstrates that coengagement of CD 55 , using either cross linking mAbs or its natural ligand CD 97 , and CD 3 results in enhanced proliferation of human peripheral blood CD 4 ( + ) T cells , expression of the activation markers CD 69 and CD 25 , and secretion of IL 10 and GM CSF . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
On days 4 , 10 , and 38 postinfection , sinus mucosa was harvested and analyzed by flow cytometry for CD 3 , CD 4 , CD 8 , CD 25 , CD11b , CCR 3 , and GR 1 positive cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The CD 4 ( + ) CD 25 ( hi ) T cell pool in the patients constitutively expressed intracellular FoxP 3 , CTLA 4 , and produced high amounts of IL 10 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Glucocorticoid amplifies IL 2 dependent expansion of functional FoxP 3 ( + ) CD 4 ( + ) CD 25 ( + ) T regulatory cells in vivo and enhances their capacity to suppress EAE . ^^^ IL 2 is crucial for the production of CD 4 ( + ) CD 25 ( + ) T regulatory ( Treg ) cells while important for the generation of effective T cell mediated immunity . ^^^ We show in this study that short term simultaneous administration of Dex and IL 2 markedly expanded functional suppressive Foxp 3 ( + ) CD 4 ( + ) CD 25 ( + ) T cells in murine peripheral lymphoid tissues . ^^^ In a myelin oligodendrocyte glycoprotein induced experimental autoimmune encephalomyelitis ( EAE ) mouse model , we observed that splenic CD 4 ( + ) CD 25 ( + ) T cells failed to suppress the proliferation of CD 4 ( + ) CD 25 ( ) T cells . ^^^ Pretreatment with Dex / IL 2 remarkably increased the proportion of CD 4 ( + ) FoxP 3 ( + ) cells and partially restored the function of splenic CD 4 ( + ) CD 25 ( + ) T cells , and inhibited the development of EAE . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Purified CD 4 ( + ) CD 25 ( + ) CD62L ( high ) B / W T ( regs ) were expanded ex vivo 80 fold , resulting in cells with a stable suppressor phenotype . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
We found that Foxp 3 ( + ) CD 4 ( + ) CD 25 ( + ) T cells are increased in the periphery but not in the thymus after Ag feeding . ^^^ These CD 4 ( + ) CD 25 ( + ) T cells also express glucocorticoid induced TNFR and intracellular CTLA 4 and suppress Ag specific proliferation of CD 4 ( + ) CD 25 ( ) cells in vitro . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
In this study , we show that i . v . injection of PTx in mice induces a decrease in the number of splenic CD 4 ( + ) CD 25 ( + ) regulatory T cells ( Treg cells ) . ^^^ Furthermore , PTx not only induces a depletion of the dominant CD 4 ( + ) CD 25 ( + ) Foxp 3 ( + ) subpopulation of splenic Treg cells , but also reduces to a similar extent the CD 4 ( + ) CD 25 ( ) Foxp 3 ( + ) subpopulation . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Increased CD 4 ( + ) CD 25 ( + ) Foxp 3 ( + ) cells were found in thoracic lymph nodes of helminth infected / OVA exposed mice . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
PKC theta deficient CD 4 ( + ) T cells show an impaired proliferative response , decreased intracellular levels of the cytokines IFN gamma , IL 2 , and IL 4 , and significantly diminished cell surface expression of the activation markers CD 25 , CD 69 , and CD134 / OX40 on memory T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
GITR is expressed at high levels on CD 4 ( + ) CD 25 ( + ) . ^^^ Currently , the majority of studies about GITR ' s role on regulatory cells are focused on CD 4 ( + ) CD 25 ( + ) Treg cells , while very little is known about the importance of this molecule in other Treg subtypes . ^^^ These early results suggest the importance of GITR in human T suppressor lymphocytes other than CD 4 ( + ) CD 25 ( + ) Treg cells . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Expression of CD 3 , CD 4 , CD 8 , HLA DR , Fas L , ICAM 1 and CD 25 were assessed using flow cytometry . mRNA of perforin , granzyme B and Fas L were quantified by real time RT PCR . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 25 ( + ) regulatory T ( T ( reg ) ) cells have emerged as central constituents of this suppressive activity . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The USPIO nanoparticles were incorporated into both the cytoplasm and nucleus of labelled cells , which retained normal in vitro proliferative responses to a polyclonal stimulus ; suppressive responses mediated by labelled CD 4 ( + ) CD 25 ( + ) regulatory T cells ; chemotactic responses to the chemokine CXCL 12 ; and transmigration of an activated endothelial monolayer . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Whereas the biology of CD 4 ( + ) CD 25 ( + ) FOXP 3 ( + ) T ( reg ) cells in murine models seems to be rather straightforward , studies in human diseases are more difficult to interpret due to expression of CD 25 on activated effector T cells as well as T ( reg ) cells . ^^^ More importantly , early studies in human tumors were mainly focused on CD 4 ( + ) CD 25 ( + ) T ( reg ) cells lacking interrogation of more specific markers such as FOXP 3 expression . ^^^ We will discuss earlier data in the context of recent findings in T ( reg ) cell biology with a particular emphasis on CD 4 ( + ) CD 25 ( high ) FOXP 3 ( + ) T ( reg ) cells in human malignancies . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Enrichment of regulatory CD 4 ( + ) CD 25 ( + ) T cells by inhibition of phospholipase D signaling . ^^^ Here we demonstrate that PLD signaling is essential for proliferation of mouse CD 8 ( + ) T cells and CD 4 ( + ) CD 25 ( ) T cells , but is not required for proliferation of CD 4 ( + ) CD 25 ( + ) regulatory T cells . ^^^ Inhibition of PLD signaling blocked effector T cell proliferation after T cell antigen receptor ( TCR ) engagement , but had no significant effect on the proliferation of CD 4 ( + ) CD 25 ( + ) T cells with regulatory functions . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
MEASUREMENTS AND MAIN RESULTS : We detected a threefold increase in the frequency of CD 4 + CD 25 ( high ) T cells ( p < 0 . 001 ) and a 2 . 2 fold increase in FoxP 3 expression ( p = 0 . 006 ) in patients with TB , and there was a positive correlation between these markers ( r = 0 . 58 , p < 0 . 001 ) . ^^^ Ex vivo depletion of CD 4 + CD 25 ( high ) cells from peripheral blood mononuclear cells resulted in increased numbers of M . tuberculosis antigen specific IFN gamma producing T cells in seven of eight patients with TB ( p = 0 . 005 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
HBcAg specific activity of regulatory T cells was evaluated by staining with antibodies to CD 4 , CD 25 , CTLA 4 and interleukin 10 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The proportion of CD 4 cells positive for CD 25 and Foxp 3 was increased , mainly in those with CD 4 cell counts less than 200 cells / microl . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Foxp 3 ( + ) CD 4 ( + ) CD 25 ( + ) regulatory T cells can differentiate from Foxp 3 ( ) CD 4 ( + ) medullary thymocytes and Foxp 3 ( ) CD 4 ( + ) naive T cells . ^^^ Here we followed the fate of individual Foxp 3 ( + ) CD 4 ( + ) CD 25 ( + ) thymocytes and T cells in vivo in T cell receptor ( TCR ) transgenic mice that express a restricted but polyclonal repertoire of TCRs . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
To assess the role of CD 4 ( + ) CD 25 ( + ) Foxp 3 ( + ) regulatory T ( Treg ) cells in overcoming immunosurveillance of Erbb 2 ( HER 2 / neu ) mammary lesions , we studied the effects of their sustained removal in BALB / c female mice made transgenic for the rat Erbb 2 ( r Erbb 2 ) oncogene ( BALB neuT mice ) , which develop multiple mammary carcinomas . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Furthermore , the effects of CpG stimulation appear to enhance the antigen presenting capacity of microglia , shift the immune response toward CD 8 ( + ) T cells , and decrease the number of CD 4 ( + ) CD 25 ( + ) regulatory T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The absolute number and proportion of CD 4 ( + ) CD 25 ( + ) cells were reduced but the proportions of the subgroup of naive regulatory T cells ( CD 4 ( + ) CD 25 ( + ) CD62L ( + ) ) and non activated regulatory T cells ( CD 4 ( + ) CD 25 ( + ) CD 69 ( ) ) were not reduced in the thymectomized children . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Because it is currently not known if regulatory T ( T ( reg ) ) cells are involved in the pathogenesis , we determined the frequency of CD 4 ( + ) CD 25 ( + ) T cells and assayed the related gene expression levels in CD 4 ( + ) CD 25 ( + ) T cells isolated from both lupus mice ( NZB / NZW F ( 1 ) ) and normal control mice ( DBA2 / NZW F ( 1 ) ) . ^^^ The results showed that the frequency of CD 4 ( + ) CD 25 ( + ) T cells in lupus mice was lower than that of normal mice . ^^^ Except for the high expression level of interleukin ( IL ) 10 mRNA , CD 4 ( + ) CD 25 ( + ) T cells from lupus mice expressed normal forkhead box P 3 ( Foxp 3 ) and transforming growth factor ( TGF ) beta mRNA , and exerted suppressive functions . ^^^ These findings indicated that CD 4 ( + ) CD 25 ( + ) T cells might be involved in the regulatory mechanism of autoantibody production . . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Antigen specific iT ( REG ) is a great counterpart of thymus derived CD 25 ( + ) CD 4 ( + ) Foxp 3 ( + ) T ( REG ) ( nT ( REG ) ) , and therefore clinical applications of T ( REG ) should be developed that integrate the advantages of both of these cell populations . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
IL 2 induced cell proliferation was significantly decreased in polarized Th 1 cells from the FO group ; however , the suppressed proliferation was not linked to reduced CD 25 surface expression on antigen stimulated CD 4 ( + ) T cells . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The eye infiltrating host CD 4 lymphocytes underwent additional changes , acquiring a profile of activated lymphocytes , i . e . , up regulation of CD 25 and CD 69 . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
The peripheral lymphocytes of the subjects were analyzed utilizing the following surface markers : CD 3 ( + ) , CD 4 ( + ) , CD 8 ( + ) , CD 19 ( + ) , CD 25 ( + ) , CD 56 ( + ) , CD 14 ( + ) , CD 16 ( + ) and HLA DR ( + ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
This was associated with an increased frequency of CD 4 ( + ) CD 25 ( + ) cells , which express Foxp 3 , in the spleen . alpha GalCer treatment just prior to Ag challenge also suppressed the development of EC in IFN gamma deficient mice , and we found apoptosis and anergy are unlikely to play a major role in the mechanism by which pre challenge alpha GalCer treatment suppresses EC . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
Isolated CD 4 ( + ) CD 25 ( + ) T cells showed a population with high density of alpha 2 , 6 linked sialic acid and a population with low expression . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
BACKGROUND : Pancreas cancer bearing mice have an increased prevalence of immunosuppressive CD 4 ( + ) CD 25 ( + ) regulatory T cells ( T ( reg ) ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
PATIENTS AND METHODS : Immunohistochemistry was used to study numbers and location of cells staining positive for immune cell markers CD 4 , CD 7 , CD 8 , CD 25 , CD 68 , forkhead box protein P 3 ( FOXP 3 ) , T cell intracellular antigen 1 , and Granzyme B in tissue microarrays of paraffin embedded , diagnostic lymph node biopsies taken from 59 FL patients who lived less than 5 years ( short survival group ; n = 34 ) and more than 15 years ( long survival group ; n = 25 ) . ^^^
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01589 and P01730	Pubmed	SVM Score :0.0
NA