| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.58009729 |
| Human pregnancy zone protein , rat alpha 1 , alpha 2 macroglobulin ( R alpha 1M , R alpha 2M ) , all homologous with H alpha 2M , were tested for their ability to bind IL 1 beta . 0.58009729^^^ |
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| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.62683877 |
| Thus , the possibility that IL 1 beta forms a disulfide bond with alpha 2M was investigated . 125I labeled human rIL 1 beta ( 15 kDa ) was incubated with fresh normal human serum or with purified alpha 2M , treated or not with methylamine . 0.62683877^^^ |
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| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| Administration of IL 1 beta to rat hepatocyte produced a similar decline of albumin secretion and a 5 fold increase of fibrinogen secretion , whereas alpha 2 macroglobulin secretion remained undisturbed . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^ |
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| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| The 125I IL 1 beta binding factors could be immunoprecipitated from TAT IC activated serum 125I IL 1 beta solutions by antisera to alpha 2 macroglobulin ( alpha 2M ) or to the third complement component ( C 3 ) . ^^^ Treatment of purified plasma alpha 2M and C 3 with trypsin or activation with methylamine , which causes cleavage of the internal thiol ester and the appearance of free thiol groups in these proteins , mediated binding of 125I IL 1 beta to alpha 2M and C3b . ^^^ The results suggest that cleavage of the internal thiol ester in C 3 and alpha 2M makes these plasma proteins susceptible to binding of 125I IL 1 beta and that free thiol groups do play a role in the formation of 125I IL 1 beta plasma protein complexes . ^^^ Activated C 3 and alpha 2M may function as IL 1 beta carrier proteins in biologic fluids , in addition to their other physiologic roles . . ^^^ |
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| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| Recent studies implicate alpha 2 macroglobulin ( alpha 2M ) as a protein that regulates the distribution and activity of many cytokines , including transforming growth factors beta ( TGFs beta ) , tumor necrosis factor alpha ( TNF alpha ) , platelet derived growth factor ( PDGF ) , interleukin 6 ( IL 6 ) , nerve growth factor ( NGF ) , fibroblast growth factor ( b FGF ) , and interleukin 1 beta ( IL 1 beta ) . ^^^ Some cytokines , including PDGF , NGF , and IL 6 bind preferentially to the native secreted form of alpha 2M , whereas the TGF beta s , TNF alpha and IL 1 beta bind preferentially to forms of alpha 2M that have been modified by proteinases such as plasmin . ^^^ |
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| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| The levels of immune complexes , complement , alpha 2 macroglobulin , acute phase proteins ( alpha 1 acid glycoprotein , C reactive protein and alpha 1 antitrypsin ) , and tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 alpha ( IL 1 alpha ) , and interleukin 1 beta ( IL 1 beta ) in the patients ' sera were within normal limits . ^^^ |
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| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| Recent immunocytochemical data have demonstrated increases in interleukin 1 beta ( IL 1 beta ) , interleukin 6 ( IL 6 ) and the IL 6 inducible acute phase protein , alpha 2 macroglobulin ( alpha 2 M ) , in Alzheimer ' s disease ( AD ) brains . ^^^ Alzheimer ' s temporal cortex , we examined mature IL 1 beta , IL 1RA , IL 6 , alpha 2 M and C reactive protein ( CRP ) . ^^^ Cytokine indices in Alzheimer ' s temporal cortex : no changes in mature IL 1 beta or IL 1RA but increases in the associated acute phase proteins IL 6 , alpha 2 macroglobulin and C reactive protein . ^^^ |
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| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| In cells treated with lipopolysaccharide ( LPS ) conditioned Raw media , interleukin ( IL ) 6 , or IL 1 beta for 24 h , there was a significant decrease of alpha 2M secretion below control levels . ^^^ |
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| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| Thus we measured the production of mitogen induced cytokines ( IL 1 beta , IL 2 , IL 6 , IL 10 , interferon gamma ) and sIL 2R in a whole blood assay , and serum protein levels such as C reactive protein ( CRP ) , haptoglobin ( Hp ) and alpha 2 macroglobulin ( alpha 2 M ) in a longitudinal 6 week study in an attempt to assess leucocyte function during and after acute clinical stage of depression in 39 patients . ^^^ |
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| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| In the present study we have applied aqueous two phase systems to analyze the interaction of IL 1 beta and alpha 2 M receptor to different forms of alpha 2 M . ^^^ The partition of IL 1 beta was changed by addition of transformed alpha 2 M to the two phase systems rather than by the native inhibitor . ^^^ The interaction between IL 1 beta and alpha 2 M was enhanced by divalent cations . ^^^ |
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| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| The plasma protein alpha 2 macroglobulin ( alpha 2M ) has been reported to bind the proinflammatory cytokines , tumor necrosis factor alpha ( TNF alpha ) and interleukin 1 beta ( IL 1 beta ) , which play a central role in the pathogenesis of chronic inflammatory disorders , including Crohn ' s disease and rheumatoid arthritis . ^^^ In this study , we chemically modified alpha 2M to stabilize a conformation of the protein ( termed MAC , Macroglobulin Activated for Cytokine binding ) with greatly increased TNF alpha and IL 1 beta binding activity . ^^^ |
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| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| Inflammatory cytokines ( IL 1 beta , IL 6 , TNF alpha ) , alpha 2M and MHCII la antigen mRNAs were not detected in the brain of SAM . ^^^ |
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| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| In all , 57 different measurements ( 30 in serum and 27 in ascitic fluid ) including erythrocyte sedimentation rate , number of white blood cells , cytokines , interleukin 1a ( IL 1a ) , IL 1b , IL 2 , IL 6 , IL 8 , tumor necrosis factor alpha , immunoglobulins ( IgG , IgA , IgM ) , complement factors C 3 and C 4 , acute phase proteins such as alpha 1 acid glycoprotein , alpha 2 macroglobulin , alpha 1 antitrypsin , haptoglobin , C reactive protein , ferritin , ceruloplasmin and transferin , were performed in 61 patients with ascites ( 25 with malignant exudates , 13 with nonmalignant exudates , and 23 with transudates ) . ^^^ |
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| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| The inhibitory effect of IL 1 beta on IL 6 induced alpha 2 macroglobulin expression is due to activation of NF kappa B . ^^^ |
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| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| NF kappaB mediates IL 1beta induced synthesis / release of alpha 2 macroglobulin in a human glial cell line . ^^^ |
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| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| Circulating levels of proinflammatory cytokines ( tumor necrosis factor alpha ( TNF alpha ) interleukin 1 beta [ IL 1 beta ] , IL 2 , IL 6 , and interferon gamma [ IFN gamma ] ) , anti inflammatory cytokines ( transforming growth factor beta 1 [ TGF beta 1 ] , IL 4 , IL 10 , and IL 13 ) , the cytokine carrier protein alpha 2 macroglobulin , IL 1 receptor antagonist ( IL 1ra ) , soluble TNF receptors ( sTNFr ) p 55 and p 75 , and soluble IL 6 receptor ( sIL 6r ) were determined in 15 patients with POEMS syndrome and 15 with multiple myeloma . ^^^ |
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| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| In vivo , carp intraperitoneally injected with T . borreli up regulate expression of TNFalpha , IL 1beta and mRNAs for acute phase response proteins ( complement factor 3 , serum amyloid A and alpha 2 macroglobulin ) . . ^^^ |
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| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| Other cytokines that bind alpha 2M ( transforming growth factor beta 1 and beta 2 , tumor necrosis factor alpha , basic fibroblast growth factor , interleukin 1 beta , and 6 ) did not compete for 125I PDGF BB binding slow alpha 2M , but transforming growth factor beta 1 and basic fibroblast growth factor inhibited 125I PDGF BB binding alpha 2M methylamine by 30 50 % . ^^^ |
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| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| They found that treating human hepatoma ( Hep G 2 ) cells with interleukin 1 ( IL 1 ) leads to the induction of alpha 1 acidglycoprotein ( AGP ) and complement 3 ( C 3 ) mRNA synthesis , and to a concomitant downregulation of albumin ( alb ) , alpha fetoprotein ( AFP ) and alpha 2 macroglobulin ( alpha 2M ) mRNA synthesis . ^^^ |
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| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| The regulation of the three major acute phase proteins alpha 2 macroglobulin , cysteine proteinase inhibitor and alpha 1 antitrypsin by recombinant human interleukin 1 beta , recombinant human interleukin 6 and recombinant human tumor necrosis factor alpha was studied in rat hepatocyte primary cultures . ^^^ Interleukin 1 beta had only a partial effect on the regulation of the four patients studied : only a twofold stimulation of alpha 2 macroglobulin and a 60 % reduction of albumin synthesis were observed . ^^^ The stimulation of alpha 2 macroglobulin and cysteine proteinase inhibitor synthesis by interleukin 6 was inhibited by interleukin 1 beta in a dose dependent manner . ^^^ |
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| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| A comparison of the interaction of interleukin 1 beta and basic fibroblast growth factor with native p alpha 2M , p alpha 2M / MA and the mutant C949Y r alpha 2M revealed that when assayed under nondenaturing conditions , no binding occurred to ' slow ' p alpha 2M whereas quantitatively similar binding was observed to ' fast ' p alpha 2M / MA and C949Y r alpha 2M . ^^^ |
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| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| Interleukin 1beta , elastase alpha 1 antitrypsin complex ( E A1AT ) , alpha 1 antitrypsin ( A1AT ) , and alpha 2 macroglobulin ( A2MG ) were measured with ELISA . ^^^ |
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| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| Binding of platelet derived growth factor BB and transforming growth factor beta 1 to alpha 2 macroglobulin in vitro and in vivo : comparison of receptor recognized and non recognized alpha 2 macroglobulin conformations . alpha 2 Macroglobulin ( alpha 2M ) undergoes a major conformational change when reacting with proteinases or primary amines . ^^^ Previous studies demonstrated that many cytokines , including transforming growth factor beta 1 ( TGF beta 1 ) and interleukin 1 beta , bind preferentially or exclusively to alpha 2M which has undergone conformational change . ^^^ In order to determine whether PDGF BB or TGF beta 1 binds to native alpha 2M , we defined the native conformation by lack of interaction with alpha 2M R / LRP instead of electrophoretic mobility . 125I PDGF BB was incubated with 4 . 3 microM native alpha 2M and 0 . 47 microM alpha 2M methylamine . ^^^ Binding of 125I PDGF BB and 125I TGF beta 1 to alpha 2M was demonstrated in vivo by injecting the free growth factors intravenously into mice . ^^^ Therefore , under normal physiological conditions , native alpha 2M ( non alpha 2M R / LRP recognized ) is the primary form of the proteinase inhibitor functioning as a carrier of PDGF BB and TGF beta 1 in the blood . . ^^^ |
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| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| Interleukin 1 beta , interleukin 6 and transforming growth factor beta synergize with hydrocortisone , increasing the synthesis of alpha 2 macroglobulin 2 to 4 fold , although they have no effect in the absence of hydrocortisone . ^^^ Interleukin 6 , unlike interleukin 1 beta or transforming growth factor beta , decreases the lag phase from 10 h to 4 h before alpha 2 macroglobulin synthesis is induced by hydrocortisone . ^^^ |
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| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| We now report that human alpha 2 macroglobulin ( alpha2M ) , a major circulating endoproteinase inhibitor , which has recently been shown to be present in senile plaques in AD , binds 125I A beta ( 1 42 ) with high affinity ( apparent dissociation constant of 3 . 8 10 10 ( 10 ) M ) . ^^^ |
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| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| Equine alpha 2M bound 125I TGF beta 1 . ^^^ However , poor or no binding was observed between alpha 2M and either of 125I rhTNF alpha or 125I IL 1 beta . ^^^ In all plasma and joint fluid samples , whether untreated or reacted with methylamine , 125I TGF beta 1 bound predominantly to alpha 2M , and preferentially to the activated form of alpha 2M . ^^^ In synovial fluid , the amount of 125I TGF beta 1 binding was proportional to the quantity of alpha 2M present . ^^^ These results indicate that : 1 ) equine alpha 2M binds TGF beta 1 ; 2 ) the native form of alpha 2M is present in both equine plasma and synovial fluid , and 3 ) alpha 2M is a major binding protein for TGF beta 1 in equine synovial fluid . ^^^ |
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| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| In pleural effusion and in plasma the following were quantified : total proteins , LDH , glucose , amylase , cholesterol , albumin , cholinesterase , phosphatase alkaline , urea , beta 2 microglobulin , IgG , IgM , alpha 2 macroglobulin , C reactive protein , transferrin , alpha 1 antitrypsin , serum amyloid A protein , interleukin 1 beta , interleukin 6 , tumoral necrosis factor alpha , and lysozyme . ^^^ |
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| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| Finally , this study associated the first appearance of myeloid cells with an immune response on the molecular level : 2 days post fertilization embryos responded to LPS injection with upregulation of interleukin 1 beta , inducible nitric oxide synthase and serum amyloid A , and down regulation of complement factor 3 and alpha 2 macroglobulin , implying a functional embryonic innate defense system . . ^^^ |
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| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01584 and P01023 |
Pubmed |
SVM Score :0.0 |
| NA |
|