Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.5624092
In order to exert its role on target cells , IFN gamma has to interact with a specific cell membrane receptor termed the IFN gamma receptor ( IFN gamma R ) . 0.5624092^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.51007128
A specific IFN gamma R 2 : IFN gamma cross linked complex was observed in cells expressing both IFN gamma R 1 and IFN gamma R 2 indicating that IFN gamma R 2 ( AF 1 ) interacts with IFN gamma and is closely associated with IFN gamma R 1 . 0.51007128^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.78609738
Mycobacterial infections are critically controlled by interferon gamma ( IFN gamma ) and the cellular responses it elaborates , as shown by patients with mutations in the IFN gamma receptor ligand binding chain ( IFN gamma R 1 ) who have disseminated nontuberculous mycobacterial infections . 0.78609738^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.71672844
The extracellular interferon gamma receptor alpha chain comprises two immunoglobulin like domains , each with fibronectin type 3 topology , which are responsible for binding interferon gamma at the cell surface . 0.71672844^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.55677614
Moreover , there was no association between IFNG , IFNGR 1 and IFNGR 2 genotypes and the combined incidence of death form any cause and non fatal myocardial infarction during the first 12 months following the intervention ( P > 0 . 61 ) . 0.55677614^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
To determine whether this was related to IFN gamma receptor ( IFN gamma R ) underexpression , binding characteristics of IFN gamma to peripheral blood lymphocytes were studied in chronic HBV infection using radioiodinated recombinant IFN gamma . ^^^ Peripheral blood lymphocytes from patients with chronic HBV infection ( n = 20 ) , normal healthy controls ( n = 12 ) and patients with non viral related chronic liver disease expressed a similar number of IFN gamma R ( medians ( ranges ) : 1891 ( 1581 2515 ) ; 1916 ( 1589 2441 ) ; 1893 ( 1692 2104 ) sites / cell , respectively , p = N . ^^^ There was no correlation between IFN gamma R expression and serum transaminase , serum HBsAg and HBV DNA titres and liver histology . ^^^ IFN alpha therapy in vivo also did not enhance IFN gamma R expression ( n = 3 ) . ^^^ There is therefore , no evidence from this data that IFN gamma R is underexpressed in patients with chronic HBV infection to account for the difference in clinical response to these two forms of therapy . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
We have studied this mechanism by examining the binding of overlapping synthetic peptides of IFN gamma to cloned soluble murine IFN gamma R . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
The number of IFN gamma R per cell rose by three and fourfold after 16 h of treatment with TNF alpha and IL 6 , respectively . ^^^ The increased surface expression of IFN gamma R induced by TNF alpha and IL 6 correlated with the rise in IFN gamma mRNA receptor levels . ^^^ TNF alpha mediated up regulation of IFN gamma R was due to an increase in transcriptional activity of the IFN gamma R gene , as shown by run on experiments . ^^^ No significant modulation of IFN gamma R gene transcription was observed in nuclei from cells treated with IL 6 , whose effect appeared to be related to IFN gamma mRNA receptor stabilization . ^^^ Taken together , the present results provide , to our knowledge , the first evidence for cytokine mediated up regulation of IFN gamma R in human monocytic cells . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Recombinant human gamma interferon ( IFN gamma ) was used to study IFN gamma receptors ( IFN gamma R ) on human CD4+ and CD8+ peripheral blood T lymphocytes . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
The distribution of human IFN gamma R ( HuIFN gamma R ) was , therefore , investigated in situ by immunohistochemistry , using affinity purified rabbit polyclonal antibodies directed against the extracellular domain of the receptor . ^^^ In lymphoid organs , IFN gamma R expression is restricted to the B cell areas of lymph nodes , adult and fetal spleen , tonsils , appendix , and mucosa associated lymphoid tissue of the small bowel . ^^^ Macrophages and other reticular cells in lymphoid tissues and other organs are strongly positive for IFN gamma R , whereas its expression was consistently negative in the cortical and medullary thymocytes . ^^^ Two color flow cytofluorometric analysis of blood , lymph node , tonsil , spleen and thymus cells confirms that most B lymphocytes are IFN gamma R positive , whereas T lymphocytes are negative . ^^^ In non lymphoid organs , IFN gamma R is expressed on endothelial cells of the medium and small size vessels . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
TGF beta also decreased the absolute number of IFN gamma R on the surfaces of pretreated macrophages by approximately 30 to 35 % , without altering the affinity with which IFN gamma bound . ^^^ These results suggest that TGF beta mediates its negative regulatory effects on macrophage activation by interfering with coupling of the IFN gamma R to the pathways that induce and maintain macrophage activation for tumor cell killing . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
We localized the IFN gamma R Ag to the trophoblasts of normal human first trimester placenta . ^^^ Jar cells expressed the IFN gamma R Ag . ^^^ These data suggest that down regulation of MHC class 2 Ag on trophoblasts in the first trimester placenta and in a cell line include a lack of productive intracellular signaling by the IFN gamma R and maintenance , in a coordinate manner , of low steady state levels of the mRNA encoded by the DRA , DRB , and invariant chain genes . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
We determined the species specificity and function of structural domains of the interferon gamma receptor ( IFN gamma R ) by construction of human / murine chimeric IFN gamma R cDNA clones and their expression in various cells . ^^^ We demonstrate that we can reconstitute a biologically active IFN gamma R in eukaryotic cells with chimeric receptors as long as the extracellular domain and an accessory factor are from the same species . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
These data collectively indicate that BALB / c mice have a non H 2 linked gene which decreases B cell sensitivity to IFN gamma , but the gene effect is not associated with the expression of IFN gamma R mRNA on B cells . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Monoclonal antibodies produced against native interferon gamma receptor ( IFN gamma R ) have been characterized for their capacity to react with purified receptor and receptor positive cells , to inhibit the binding of IFN gamma to cellular receptor , to precipitate the receptor protein when cross linked to IFN gamma , and to recognize the recombinant interferon gamma receptor and 19 overlapping fragments of this protein expressed in Escherichia coli . ^^^ The results of this analysis showed that : ( 1 ) the extracellular portion of human IFN gamma R is located between the N terminus and the transmembrane region ( amino acids 18 246 ) . ( 2 ) The intracellular domain is between the transmembrane region and the C terminus ( amino acids 269 489 ) . ( 3 ) The monoclonal antibodies that react with the IFN gamma R intracellular domain recognize small linear epitopes . ( 4 ) The human IFN gamma R binding site is located between the N terminus and the transmembrane region . ( 5 ) The monoclonal antibodies that react with IFN gamma R extracellular domain and inhibit the binding of IFN gamma recognize two different epitopes . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
This study investigates the ability of the human monocyte like cell line , U 937 , to regulate the cell surface expression of the IFN gamma receptor ( IFN gamma R ) during endocytosis of ligand . ^^^ Recombinant IFN gamma was radiolabelled to high specific radioactivity with Bolton Hunter reagent and used to enumerate IFN gamma R on treated U 937 cells . ^^^ Cells which had internalized IFN gamma for up to 3 h displayed maximal levels of IFN gamma R at all time points tested after all unlabelled IFN gamma had been acid stripped from the cell at pH 2 . 78 . ^^^ After trypsin treatment of the IFN gamma R , the cells were able to synthesize and insert into the cell membrane up to 1000 IFN gamma R molecules / h after a 60 min lag . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
The protein was detectable by a mAb that is specific for an epitope that is found in the ligand binding site of the receptor for mouse IFN gamma , as well as by a goat polyclonal IgG that is monospecific for the mouse IFN gamma R . ^^^ Supernates that contained the secreted protein blocked binding of IFN gamma to mouse IFN gamma R and inhibited in a dose dependent manner the IFN gamma mediated priming of mouse bone marrow culture derived macrophages for tumor cell killing . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
The role of physiologically secreted human IFN gamma in T lymphocyte and NK cell activation has been probed with a panel of mouse mAb directed against various epitopes of the human IFN gamma molecule , or human IFN gamma R . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
In this report we define the IFN gamma receptor ( IFN gamma R ) on human monocytes as a receptor complex consisting of at least three subunits . ^^^ This enabled subunits to be coimmunoprecipitated by several different anti IFN gamma R antibodies raised against the 90 kDa cloned binding protein . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Expression of the human ( hu ) IFN gamma R has been studied in Raji and IM 9 cells ( two B lymphoblastoid cell lines ) and in THP 1 cells ( a monocytic cell line ) with respect to IFN gamma binding sites , receptor protein and mRNA levels . ^^^ Although , in these three cell lines , the hu IFN gamma R mRNA was expressed to the same extent , the high affinity receptor was expressed differently both in cell surface receptor binding and amount of receptor protein . ^^^ We investigated the modulation of the hu IFN gamma R by its ligand . ^^^ Hu IFN gamma induced a rapid and dose dependent decrease of its cell surface receptor number without alteration of receptor affinity , amounts of receptor protein or hu IFN gamma R mRNA accumulation and stability . ^^^ Therefore , the modulation of the hu IFN gamma R expression by PMA , a potent activator of protein kinase C and a modulator of other receptor expression , has been investigated . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Affinity chromatography of crude human urinary proteins on either human recombinant interleukin 6 ( rIL 6 ) or human recombinant interferon gamma ( rIFN gamma ) or anti IFN gamma receptor ( IFN gamma R ) monoclonal antibodies ( McAb ) yielded the two respective soluble receptors in significant amounts . ^^^ Analysis of the eluted proteins from both IFN gamma and anti IFN gamma R columns by inhibition of solid phase radioimmunoassay , enzyme linked immunosorbent assay , sodium dodecyl sulphate polyacrylamide gel electrophoresis and Western blotting proved the existence of soluble IFN gamma R in normal urine . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
The regulation of human IFN gamma receptor ( IFN gamma R ) expression by granulocyte macrophage CSF ( GM CSF ) was investigated . ^^^ Scatchard plot analyses revealed that down regulation was caused by a decrease in IFN gamma R number rather than by a change in affinity . ^^^ GM CSF treatment did not reduce IFN gamma R specific mRNA levels , but reduced the half life of membrane expressed IFN gamma R , indicating a post translational control of IFN gamma R by GM CSF . ^^^ Because both IFN gamma and GM CSF are crucially involved in activation of monocytic function , the data presented suggest that down regulation of IFN gamma R by GM CSF may represent a potential negative feedback control of monocyte activation . ^^^ Further studies of IFN gamma binding characteristics and isolation of IFN gamma R by immunoprecipitation revealed that IFN gamma binding to human peripheral blood monocytes is mediated by a receptor protein structurally and functionally identical to that previously characterized in several established cell lines of other tissue origin . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Treatment of macrophages with IFN gamma increased the specific binding of IL 2 ; treatment of cells with IL 2 , however , did not up regulate the IFN gamma R . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Affinity chromatography of crude human urinary proteins on either human rIL 6 , human rIFN gamma , or anti IFN gamma R mAb yielded the two respective soluble receptors in significant quantities . ^^^ Analysis of the eluted proteins from both IFN gamma and anti IFN gamma R columns by inhibition of solid phase RIA , ELISA , SDS PAGE , and Western blotting proved the existence of soluble IFN gamma R in normal urine . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Interferon gamma receptors ( IFN gamma R ) exhibit remarkable species specificity . ^^^ In order to understand the basis for this phenomenon , we have isolated a recombinant cDNA clone corresponding to the mouse ( Mu ) IFN gamma R . ^^^ Sequence analysis of the cDNA encoding the Mu IFN gamma R and comparison with the corresponding human IFN gamma R sequence shows about 68 % conservation of the extracellular domains and 51 % conservation of the cytoplasmic domains at the nucleotide level . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Purified preparations of the human IFN gamma R derived from placental membranes were used to produce receptor specific murine mAb . ^^^ Two of these ( GIR 208 and GIR 301 ) abrogated all binding of radioligand to either intact placental membranes or soluble , purified IFN gamma R . ^^^ Competition experiments revealed that GIR 208 and GIR 301 recognized similar epitopes on the IFN gamma R and that these ( or this ) epitopes were identical to or linked to the ligand binding site of the receptor . ^^^ These data thus indicate that the IFN gamma R expressed on human placental cells , mononuclear phagocytes , and fibroblasts are similar . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
We have used human rodent somatic cell hybrids to investigate the regional localization of the IFN gamma R gene on human chromosome 6 and studied functional and antigenic characteristics of the expressed IFN gamma R by Scatchard analyses of 125I IFN gamma binding and binding of an anti receptor mAb ( A6C5 ) . ^^^ The data obtained revealed coordinate expression of IFN gamma and A6C5 binding capacity as well as competition in binding to chromosome 6 positive hybrids and normal cells , indicating that the A6C5 defined protein is by itself capable of high affinity IFN gamma binding and , thus , is likely to constitute the major IFN gamma R protein of distinct cell types . ^^^ Genetic linkage of the IFN gamma R gene to an oncogene located in a region of non random chromosomal aberrations may have a causal relationship to the deregulated IFN gamma R expression in several malignancies . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Moreover , they indicate that the regulatory elements for this gene are completely preserved over the phylogenetic distance separating mouse and man , even though substantial drift has occurred in the structural gene , and probably in the IFN gamma R as well . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
The role of interferon gamma ( IFN gamma ) in contact hypersensitivity induced by the haptens , oxazolone and 2 , 4 , 6 trinitrochlorobenzene ( TNCB ) , was investigated in mice with a targeted disruption of the IFN gamma receptor ( IFN gamma R / ) . ^^^ The hapten induced upregulation of intercellular adhesion molecule 1 ( ICAM 1 ) and major histocompatibility complex ( MHC ) class 1 in IFN gamma R / mice was smaller when compared to wild type mice . ^^^ Therefore , reduced cellular infiltrates are likely due to a reduced upregulation of ICAM 1 and class 1 antigen expression in IFN gamma R / mice . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
IL 12 induced organ alterations are reported for mice and the pathogenetic role of IFN gamma is investigated by the use of mice deficient in the IFN gamma receptor ( IFN gamma R / ) . ^^^ IL 12 induced a qualitatively similar macrophage infiltration in IFN gamma R / mice , less marked splenomegaly ( to 2 10 normal ) , and no MHC upregulation . ^^^ Strikingly increased vascular endothelial intercellular adhesion molecule 1 expression was apparent in both IFN gamma R / and IFN gamma R+ / + mice . ^^^ Extensive quantitative reverse transcriptase polymerase chain reaction analysis revealed an increase of only IL 6 and IL 10 pulmonary gene transcripts in IFN gamma R / mice compared with wild type mice . ^^^ The pulmonary pathology in IFN gamma R / mice , however , reveals a toxic potential for IL 12 and suggests that endogenous IFN gamma plays a protective role in preventing fatal pulmonary disease in these mice . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Previously , we identified a species nonspecific binding site for the C terminus of IFN gamma , encompassed by IFN gamma peptide IFN gamma ( 95 133 ) , on the membrane proximal region of the cytoplasmic domain of the IFN gamma R alpha chain . ^^^ Using both a radioligand binding assay and coimmunoprecipitation with antireceptor antiserum , we were able to demonstrate specific interaction of JAK 2 with the murine IFN gamma R ( MIR ) alpha chain . ^^^ These data show site specific interaction between JAK 2 and IFN gamma with the IFN gamma R and have broader implications for the role of the IFN gamma ligand in the IFN gamma signal transduction pathway . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
The role of IFN gamma in the regulation of host resistance of Staphylococcus aureus was studied using IFN gamma receptor deficient ( IFN gamma R / ) mice in a model of S . aureus induced septicemia and arthritis . ^^^ IFN gamma R / mice and wild type controls were inoculated intravenously with a toxic shock syndrome toxin 1 producing S . aureus LS 1 strain . ^^^ IFN gamma R / mice displayed significantly more frequent and more severe arthritis compared with wild type littermates ( p < 0 . 01 ) throughout the course of infection . ^^^ Notably , IFN gamma R / mice developed severe sepsis with high mortality early after the inoculation with staphylococci . ^^^ However , the mortality of wild type mice became significantly higher at later stages of the disease compared with IFN gamma R / mice ( p < 0 . 05 ) . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
The receptors studied were interleukin ( IL ) 1 receptor type 1 ( IL 1RI ) and type 2 ( IL 1RII ) , p 75 and p 55 tumor necrosis factor ( TNF ) receptors ( p75TNFR and p55TNFR ) , interferon ( IFN ) alpha / beta and gamma receptors ( IFN alpha / beta R and IFN gamma R ) , granulocyte macrophage ( GM ) colony stimulating factors receptor alpha subunit ( GM CSFR ) , G CSF receptor ( G CSFR ) , M CSF receptor ( c fms , M CSFR ) , stem cell factor receptor ( c kit , SCFR ) , IL 6 receptor ( IL 6R ) , and IL 8 receptor ( IL 8R ) . ^^^ Transcripts for IL 1RI , p55TNFR , IFN alpha / beta R , and IFN gamma R were present in all cell lines . ^^^ Normal astrocytes were positive for IL 1RI , p75TNFR , p55TNFR , IFN alpha / beta R , IFN gamma R , M CSFR , and SCFR , showing a similarity to glioblastoma cells . ^^^ Of the 11 glioblastoma tissue specimens , IL 1RI was positive in 11 , IL 1RII in 10 , p75TNFR in nine , p55TNFR in nine , IFN alpha / beta R in 10 , IFN gamma R in 10 , GM CSFR in two , G CSFR in three , IL 8R in eight , and M CSFR and SCFR in 11 . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
T cell independent and T cell dependent B cell activation increases IFN gamma R expression and renders B cells sensitive to IFN gamma mediated inhibition . ^^^ The LPS activation of conventional and CD5+ B cells resulted in increased IFN gamma R expression and increased the ability of IFN gamma to inhibit LPS induced B cell differentiation correlated with increased IFN gamma R expression . ^^^ We detected increased B cell IFN gamma R expression 12 h after activation , and maximal IFN gamma R expression was observed at 24 h . ^^^ Activation of B cells by F ( ab 2 ) ' anti IgM induced a similar increase in IFN gamma R expression . ^^^ In autoimmune New Zealand Black mice , both conventional and CD5+ B cells showed a pattern of IFN gamma R expression similar to that seen in DBA / 2 mice , and both populations of B cells were sensitive to inhibition by IFN gamma . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Although the tyrosine kinases , Jak 1 and Jak 2 , have recently been shown to be critical for signal transduction by IFN gamma , evidence is lacking for both tyrosine phosphorylation of the IFN gamma receptor ( IFN gamma R ) and the interaction between Jak 1 , Jak 2 , and the IFN gamma R . ^^^ In this report , we show that binding of IFN gamma to HeLa cells initiated a series of events that resulted in the extremely rapid ( 15 s ) tyrosine phosphorylation of not only Jak 1 , Jak 2 , and p 91 but also the IFN gamma R . ^^^ Coimmunoprecipitation experiments revealed that Jak 1 was associated with the IFN gamma R prior to ligand binding , whereas Jak 2 became part of the IFN gamma R Jak 1 complex immediately after ligand binding . ^^^ H2O2 / vanadate treatment of cells for 15 min resulted in only the tyrosine phosphorylation of Jak 1 and IFN gamma R . ^^^ These data suggest that JAK 1 associates with the IFN gamma R prior to ligand binding . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
IFN gamma receptor deficient ( IFN gamma R / ) mice , immunized with different developmental stages of malaria parasites , were used to define the mechanisms of protection against the various stages of this infection . ^^^ IFN gamma R / mice failed to develop protective immunity against Plasmodium yoelii sporozoites or liver stages , upon immunization with a single dose of irradiated sporozoites , whereas in immunized wild type mice , parasite development was strongly inhibited . ^^^ Immunized wild type mice expressed high levels of inducible nitric oxide synthase ( iNOS ) mRNA in their liver , upon challenge with viable sporozoites , whereas only background levels of iNOS were detected in immunized IFN gamma R / mice . ^^^ In contrast , after immunization with multiple doses of irradiated sporozoites , both IFN gamma R / and wild type mice mounted an immune response , which strongly inhibited the development of liver stage parasites . ^^^ As for the course of the erythrocytic phase of infection by nonlethal malaria species , P . yoelii yoelii and P . chabaudi adami , we observed only a moderately prolonged parasitemia in IFN gamma R / mice compared with wild type mice , indicating that IFN gamma may only play a modest role in immunity against erythrocytic stages . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Total RNA was prepared from the days 7 embryos by acid guanidinium thiocyanate phenol chloroform ( AGPC ) method . cDNA was synthesized by M MLV reverse transcriptase , and amplified using the specific oligonucleotide primers for IL 1 , IL 2 , IL 3 , IL 4 , IL 5 , IL 6 , TNF alpha , IFN alpha , IFN beta , IFN gamma , IFN alpha / beta receptor ( IFN alpha / beta R ) , IFN gamma receptor ( IFN gamma R ) , interferon reguratory factor ( IRF ) 1 , IRF 2 and 2 ' 5 ' oligoadenylate synthetase ( 2 5AS ) by PCR method . ^^^ On the other hand , the expressions of IFN related genes such as IFN alpha / beta R , IFN gamma R , IRF 1 , IRF 2 and 2 5AS mRNA , were also detected . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Mice with a disruption of the IFN gamma receptor alpha chain gene ( IFN gamma R alpha o / o mice ) were found to be significantly more sensitive than their wild type counterparts to induction of the anti CD 3 induced disease syndrome . ^^^ Specifically , when given a selected dose of anti CD 3 Ab , IFN gamma R alpha o / o mice developed severe hypothermia and hypoglycemia , leading to 100 % mortality within 72 h . ^^^ Other histologic alterations were steatosis in livers , and erythrocyte extravasation and infiltration of apoptotic cells in lungs , all of which were exclusively observed in IFN gamma R alpha o / o mice . ^^^ Blood levels of TNF , IL 2 , IL 6 , and IL 10 were slightly more elevated in IFN gamma R alpha o / o mice , but insufficiently so to explain increased disease severity . ^^^ Increased susceptibility of IFN gamma R alpha o / o mice was associated with impaired nitric oxide ( NO ) production , as indicated by significantly lower plasma nitrite levels and by more transient expression of spleen inducible NO synthase mRNA . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
We previously discovered that the IFN gamma receptor ( IFN gamma R ) was able to bind IFN gamma , but required one or more accessory factors to exhibit functional activity . ^^^ The presence of the Hu IFN gamma R and the human accessory factor 1 ( designated AF 1 ) are both necessary and sufficient to induce MHC class 1 antigens in response to Hu IFN gamma in hamster cells . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Here , we used interferon ( IFN ) gamma receptor deficient ( IFN gamma R / ) mice to investigate whether the in vivo inhibition of hematopoiesis by IL 12 is indirectly mediated by IL 12 induced IFN gamma . ^^^ IL 12 administered for 4 d ( 1 microgram / mouse per day ) resulted in lower peripheral blood counts and a 2 fold decrease in bone marrow cellularity in wild type mice , but not in IFN gamma R / mice . ^^^ Bone marrow hematopoietic progenitors were decreased after IL 12 treatment in wild type mice , but rather increased in IFN gamma R / mice . ^^^ In IFN gamma R / mice , spleen cellularity was less increased , there were fewer infiltrating NK cells , but a strong extramedullary hematopoiesis . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
The extracellular portion of the human interferon gamma receptor ( IFN gamma R ) is predicted to adopt two Ig like domains each with Ig superfamily type C 2 topology . ^^^ Surface plasmon resonance technology has been used here to compare the equilibrium and kinetic constants for binding reactions between these and several mutant domains , and neutralising anti IFN gamma R monoclonal antibodies . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
This unique broad species specificity of the IFN gamma R would aid virus replication in different species and suggests that vaccinia , cowpox , and camelpox viruses may have evolved in several species , possibly including humans but excluding mice . ^^^ Last , the conservation of an IFN gamma R in orthopoxviruses emphasizes the importance of IFN gamma in defense against poxvirus infections . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
The IFN gamma inducible signal transducing factor ( STF IFN gamma ) was activated in murine TH 2 but not in TH 1 cell clones , because in the latter the second chain of the IFN gamma receptor ( accessory factor 1 or IFN gamma R beta ) was absent . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
A substantial proportion of human peripheral blood mononuclear cells ( PBMC ) manifested a decreased capacity to express membrane interferon gamma receptors ( IFN gamma R ) when co cultured with Trypanosoma cruzi . ^^^ Among the lymphocytes , B cells accounted for the bulk of this effect , evidenced by a marked drop in the proportion of CD19+ or CD20+ cells expressing IFN gamma R . ^^^ Decreased IFN gamma R expression by B lymphocytes was seen as early as 3 h after co culture with T . cruzi and persisted for at least 24 h . ^^^ In PBMC cultures stimulated with Staphlyococcus aureus Cowan 1 ( SACI ) , T . cruzi decreased the percentages of both IFN gamma R+ and IFN R+bright ( cells expressing above normal levels of surface IFN gamma R ) B lymphocytes . ^^^ Cell free filtrates of T . cruzi suspensions reproduced the suppressive effects of living parasites on IFN gamma R expression by B cells . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Signaling by interferon gamma ( IFN gamma ) requires two structurally related cell surface proteins : a ligand binding polypeptide , known as the IFN gamma receptor ( IFN gamma R ) , and an accessory factor . ^^^ However , it is not known whether IFN gamma forms a ternary complex with the IFN gamma R and accessory factor to initiate signaling . ^^^ We observe complexes containing ligand , two molecules of IFN gamma R ( designated the IFN gamma R alpha chain ) , and one or two molecules of accessory factor ( designated the IFN gamma R beta chain ) . ^^^ Transfected cells expressing both IFN gamma R chains bind IFN gamma with higher affinity than do cells expressing alpha chain alone . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
As IFN gamma exerts its biological functions through specific cell surface receptors and a great number of IFN gamma receptors ( IFN gamma R ) have been purified from human placenta , we have examined the relative distribution of IFN gamma and IFN gamma R in human placental tissues at different stages of pregnancy . ^^^ By using immunohistochemical analysis and monoclonal antibodies , it was found that IFN gamma expression is intense in the first trimester but almost imperceptible at term , whereas the expression of IFN gamma R is present at both stages of pregnancy . ^^^ From these results it appears that the expression of IFN gamma R in trophoblast is related to the presence of the lymphokine in the early phase of gestation but not later . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
These data suggest that an IFN gamma inhibitor , such as the soluble IFN gamma R , can be used for SLE therapy in the early stages of the disease . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
The extracellular interferon gamma receptor alpha chain ( IFN gamma R ) is believed to comprise two discrete approximately 110 amino acid immunoglobulin like domains , perhaps similar to those seen in the crystal structure of the extracellular human growth hormone receptor [ De Vos , A . ^^^ In accord with this idea , we show that these IFN gamma R immunoglobulin like domains can be produced separately in a soluble form with a native like fold . ^^^ Cleavage of the fusion protein by enterokinase released the receptor fragment ( approximately 12 kDa ) , which was recognized by several neutralizing antibodies with affinities , measured using surface plasmon resonance technology , that were essentially indistinguishable from those seen with the full length extracellular IFN gamma R produced in eukaryotic cells . ^^^ The second membrane proximal Ig like domain of the IFN gamma R ( residues 90 229 ) was produced , albeit less efficiently , and characterized in a similar way . ^^^ The production of these two independently folded proteins provides experimental support for the two domain organization of the IFN gamma R and opens new avenues for structural studies on these Ig like molecules by NMR and crystallographic methods . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
The purpose of this investigation was to characterize the gene that encodes the receptor for mouse interferon gamma ( IFN gamma R ) , including determination of its size , intronic boundaries and its transcription start points ( tsp ) . ^^^ The mouse IFN gamma R gene is 22 kb long , with six introns that range in size from approx . 1 to 7 kb . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
In order to evaluate the potential of placental cells to bind the multifunctional cytokine , interferon gamma ( IFN gamma ) , tissues collected from pregnant Swiss mice were analysed for IFN gamma receptor ( IFN gamma R ) mRNA and protein . ^^^ Northern blot hybridization studies indicated that the relative abundance of IFN gamma R mRNA increased as gestation progressed to term . ^^^ Analysis by in situ hybridization revealed that trophoblast cells first contained high steady state levels of IFN gamma R mRNA at g . d . 12 . ^^^ These cells also contained immunoreactive IFN gamma R protein . ^^^ By g . d . 18 , IFN gamma R mRNA was clearly detectable in large spongiotrophoblast cells and labyrinthine trophoblast . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
We studied its significance in the development of antiviral immune responses by using IFN gamma receptor deficient ( IFN gamma R / ) mice . ^^^ However , the absence of the IFN gamma R resulted in increased proliferative splenocyte responses . ^^^ After immunization with either live attenuated or inactivated PRV , IFN gamma R / mice produced significantly less antiviral Ab , and more succumbed to challenge infection than the intact control animals . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
We here describe an efficient procedure for overexpression and purification of recombinant complete human interferon gamma ( IFN gamma ) receptor ( IFN gamma R ) and its extracellular fragment employing a baculovirus ( BV ) expression system . ^^^ Infection of Sf 158 cells with recombinant BV results in membrane expression of high affinity IFN gamma R ( Kd 1 . 6 10 10 ( 10 ) M ) , with approximately 10 ( 6 ) molecules / cell 40 h postinfection . ^^^ Solubilized , affinity purified IFN gamma R and a secreted extracellular domain of IFN gamma R were compared for ligand binding capacity and antagonistic activity in an IFN gamma bioassay . ^^^ This suggests that the transmembrane and cytoplasmic domains of IFN gamma R contribute to stability and / or enhance formation of biologically active receptor complexes in solution . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Expression of the human interferon gamma receptor ( IFN gamma R ) in mouse cells is not sufficient to confer biological responsiveness to human IFN gamma and vice versa . ^^^ We identified this cofactor by expression cloning in simian COS cells stably transfected with the nonfunctional murine IFN gamma R and a IFN gamma inducible reporter construct encoding the human Tac antigen ( interleukin 2 receptor alpha chain , CD 25 ) . ^^^ A cDNA clone was obtained that , upon stable transfection , rendered human HEp 2 cells expressing the murine IFN gamma R fully responsive to murine IFN gamma . ^^^ This cDNA encodes a novel 332 amino acid type 1 transmembrane protein that belongs to the IFN receptor family and that we designate IFN gamma R beta chain . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
The advent of IFN gamma receptor negative ( IFN gamma R / ) mutant mice has enabled a more direct assessment of the role of IFN gamma in endotoxin ( lipopolysaccharide [ LPS ] induced shock . ^^^ We report that IFN gamma R / mice have an increased resistance to LPS induced toxicity , this resistance manifesting well before the synthesis and release of LPS induced IFN gamma . ^^^ LPS induced lymphopenia , thrombocytopenia , and weight loss seen in wild type mice were attenuated in IFN gamma R / mice . ^^^ IFN gamma R / mice tolerated 100 1 , 000 times more LPS than the minimum lethal dose for wild type mice in a D galactosamine ( D GalN ) / LPS model . ^^^ Bone marrow and splenic macrophages from IFN gamma R / mice had a four to sixfold decreased LPS binding capacity which correlated with similar reduction in CD 14 . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
The ligand binding chain of the interferon gamma receptor ( IFN gamma R ) is a unique cell surface protein which has no similarities to other cytokine receptors . ^^^ We and others have shown that IFN gamma mediated signal transduction requires a species specific interaction of the extracellular portion of the known IFN gamma receptor alpha chain with an additional receptor subunit that was cloned recently and designated IFN gamma R beta chain or accessory factor 1 . ^^^ A cell line derived from embryos that lack the IFN gamma R alpha subunit was reconstituted with a hybrid mouse human alpha subunit that consisted of an extracellular murine and transmembrane and cytoplasmic human domains . ^^^ The experiments reported herein showed that in mouse cells , the human intracellular domain of the hybrid IFN gamma R alpha subunit was fully functional and that , therefore , signaling steps involving this domain are not species specific . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
In order to evaluate potential receptivity of these cells to interferon gamma ( IFN gamma ) , tissues taken from cycling and pregnant mice were tested for IFN gamma receptor ( IFN gamma R ) mRNA and protein . ^^^ In cycling uteri , the abundance of IFN gamma R mRNA relative to an invariant message ( glyceraldehyde 3 phosphate dehydrogenase ) increased during progression of the hormonally regulated estrous cycle . ^^^ IFN gamma R mRNA in situ hybridization signals were slightly higher in macrophage like than in other types of endometrial stromal cells . ^^^ In pregnant uteri , the highest proportions of IFN gamma R mRNA were observed at gestation day ( g . d . ) 16 . ^^^ IFN gamma R expression in both lineages remained stable through the balance of pregnancy . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Since IL 10 inhibits macrophage activation by IFN gamma , a possible implication of this relationship interaction of IL 10R and IFN gamma R or their signaling pathways . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
IFN gamma R expression is subject to contrasting modulation on human T cells . ^^^ IFN gamma R constitutive expression is low on three human malignant T cells ( ST 4 , PF 382 , and Jurkat ) growing in medium supplemented with serum . ^^^ The addition of IFN gamma down modulates IFN gamma R expression and increases both proliferation and MHC class 1 Ag expression . ^^^ By contrast , when malignant T cells are cultured in medium without serum , IFN gamma R expression dramatically increases and the cells undergo a slow apoptotic death . ^^^ This opposite ability of IFN gamma to stimulate malignant T cell proliferation or death correlates with the intensity of IFN gamma R cell expression , high expression being a marker for cell apoptosis . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
The mechanism coupling the IFN gamma receptor ( IFN gamma R ) to PLA 2 activation is not clearly defined , and no information is available on this mechanism in neuroblast cells . ^^^ We have tested the hypothesis that GTP binding proteins ( G proteins ) may couple the IFN gamma R to PLA 2 in the human neuroblastoma ( NB ) cell line LAN 5 . ^^^ IFN gamma stimulated AA release was still observed in permeabilized cells that were blocked by pretreatment with anti IFN gamma R antibodies . ^^^ A role for G proteins in IFN gamma R coupling to PLA 2 was further supported by the inhibition of IFN gamma induced [ 3H ] AA release by treatment of permeabilized cells with pertussis toxin and with the antiserum against the common alpha subunits of G proteins . ^^^ Altogether , these findings suggest the existence of IFN gamma R , which couples a Ca ( 2+ ) independent PLA 2 activation via pertussis toxin sensitive G proteins . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
In addition , we noted an increase in the cytokine and growth factor receptors TNF R , IL 1R type 2 , IL 2R , IFN gamma R and PDGF beta R . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
In this report we show that culture of human peripheral blood monocytes for up to 6 days in the presence of GM CSF results in an eightfold increase in the level of IFN gamma R expression , as determined by radioligand binding . ^^^ The IFN gamma R on these cells maintains a specificity typical of that observed in fresh monocytes . ^^^ Only IFN gamma , not IFN alpha or beta , blocks the binding of IFN gamma to its receptor , and anti IFN gamma R antibodies block at least 80 % of binding of IFN gamma to these cultured cells . ^^^ Therefore , suppression of IFN gamma mediated IP 10 induction is not the result of a global abrogation of signal transduction across the IFN gamma R but a more selective inhibition that appears to occur downstream of the receptor . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
No significant immunogenicity was detected after multiple chronic injections of mouse ( Mo ) IFN gamma R or injections of MoIFN gamma R in emulsion with CFA . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Despite a blood half life of only 1 3 hr as demonstrated in pharmacokinetic experiments , the mouse soluble IFN gamma R was able to modify the onset of acute GVHD ( alloreaction ) and chronic GVHD ( lupuslike disease ) . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
In this study , the kinetics of the surface expression of the 90 kDa IFN gamma receptor ( IFN gamma R ) was followed during human mixed lymphocyte reaction ( MLR ) to alloantigens . ^^^ IFN gamma R mRNA is constitutively expressed on resting peripheral blood lymphocytes emerging from nylon wood column ( NW PBL ) and its expression increases two to threefold on alloactivated NW PBL . ^^^ IFN gamma R protein is poorly expressed on the membrane of resting CD3+ cells , but up modulates after 3 day MLR and sharply down modulates at day 6 . ^^^ Both the p 55 and the p 75 chains of interleukin 2 receptor ( IL 2R ) were shown to up modulate in parallel with IFN gamma R , whereas they were still highly expressed at day 6 . ^^^ After alloactivation , IFN gamma and IL 2 secretion starts at 24 h , peaks at day 3 and decreases just when IFN gamma R and IL 2R begin to up modulate . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
In vivo depletion experiments revealed that IL 12 accounts for Yersinia induced IFN gamma production by both NK cells and CD4+ T cells , the latter of which are an essential source of IFN gamma r while NK cell derived IFN gamma production can be compensated by other cells . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
We constructed this immunoadhesin by linking the extracellular portion of the mouse IFN gamma R to the hinge and Fc region of an IgG 1 heavy chain . ^^^ Murine IFN gamma R IgG is secreted by transfected cells as a disulphide bonded homodimer which binds IFN gamma bivalently , with high affinity and in a species specific manner . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Here we show that H 21 A beta / mice ( lacking MHC class 2 molecules and thus devoid of mature CD4+ TCR alpha beta cells ) , TCR beta / mice ( devoid of TCR alpha beta cells ) , and IFN gamma R / mice ( unresponsive to IFN gamma ) are highly susceptible to S . typhimurium aroA infection compared with heterozygous controls . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Murine monoclonal antibodies ( mAbs ) are described that recognize the extracellular human interferon gamma receptor alpha chain ( IFN gamma R ) and inhibit the binding to it of interferon gamma . ^^^ The mAbs A 6 and gamma R 38 recognize overlapping epitopes on the N terminal Ig like domain of the IFN gamma R , whereas the gamma R 99 epitope is located largely in the membrane proximal Ig like domain . ^^^ These studies provide essential information for crystallographic and mutagenesis experiments aimed at understanding the molecular basis of the interactions of these mAbs with the extracellular IFN gamma R . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Mice with disrupted IFN gamma genes have reduced parasitemia and prolonged survival , while the outcome is reversed in mice that lack the IFN gamma R gene . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
With the use of chimeric receptors involving the interferon gamma receptor ( IFN gammaR ) complex as a model system , we demonstrate that Jak 2 activation is not an absolute requirement for IFN gamma signaling . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
We tested the existence of alternative pathways of MHC class 2 regulation using IFN gamma receptor deficient ( IFN gamma R / ) mice . ^^^ Since injection of IFN gamma induced proximal tubular MHC class 2 expression in wild type mice but not in IFN gamma R / mice , an alternative signaling pathway for IFN gamma does not seem to exist . ^^^ The markedly patchy pattern of immunofluorescence in IFN gamma R / mice suggests that induction of MHC class 2 after LPS injection may represent renal injury due to shock . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
To investigate the modulatory role of IFN gamma on the induction and maintenance of Th 2 mucosal immunity in vivo , experiments were performed in mice lacking the IFN gamma R . ^^^ However , while immunized IFN gamma R deficient mice exhibited elevated levels of IgE , IgG 1 , and reduced levels of IgG2a compared with wild type mice , there was no difference in the recruitment of eosinophils into the lung or the production of IL 4 and IL 5 from lung T cells on day 3 . ^^^ In contrast , up to 2 mo after a single Ag challenge , eosinophils were still present in the lungs of IFN gamma R deficient , but not wild type , mice . ^^^ Likewise , lung derived T cells from IFN gamma R deficient mice produced higher levels of IL 4 and IL 5 , both at 1 and 2 mo after OVA challenge compared with T cells from wild type mice . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
The role of this cytokine has been investigated in mice with a targeted disruption of the IFN gamma receptor gene ( IFN gamma R / mice ) . ^^^ In contrast the IFN gamma R / mice showed a massive and generalized leucocytic infiltration of the airways and interstitium in which eosinophils were a prominent feature . ^^^ Cultures of airway leucocytes from C57BL / 6 mice produced abundant IFN gamma whilst those from IFN gamma R / mice produced interleukin 4 ( IL 4 ) , IL 5 and IL 10 , indicating default to the Th 2 pathway ; the WT animals showed an intermediate response . ^^^ The loose but intense infiltrate of leucocytes in the lungs of IFN gamma R / mice was clearly ineffective in eliminating challenge parasites , whereas the level of IFN gamma protein and mRNA in the lungs of C57BL / 6 and WT mice correlated with the size and compactness of effector foci . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Although the majority of fLC were positive for the alpha chain of GM CSF receptor ( GM CSFR ) , the beta chain of GM CSFR was detected only on 15 % of CD1a+ cells . fLC were also positive for IL 1 receptor ( IL 1R ) type 1 , IL 1R type 2 , 75 , 000 molecular weight TNF receptor ( TNFR ) and interferon gamma receptor ( IFN gamma R ) . ^^^ In contrast , IL 1R type 1 and 75 , 000 molecular weight TNFR were down modulated and the expression of IFN gamma R was not affected by culture . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Three single chain antibody fragments that recognize the extracellular human interferon gamma receptor alpha chain ( IFN gamma R ) , and inhibit the binding of human IFN gamma , have been produced in Escherichia coli . ^^^ Using surface plasmon resonance technology ( BIAcore ) , the soluble proteins were shown to retain a high affinity for recombinant IFN gamma R , and by radioimmunoassay to possess a high inhibitory activity towards IFN gamma binding to human Raji cells . ^^^ Attempts to dissect further the antibodies to isolated VH and VL chains and to synthetic linear and cyclic peptides derived from the individual complementarity determining regions failed to afford fragments with significant IFN gamma R binding affinity . ^^^ Nevertheless , these native like variable region fragments and petidomimetics derived from them are of interest in the design of novel IFN gamma R antagonists . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
We describe here the bioengineering of a bivalent IFN gamma RFc immunoadhesin consisting of the extracellular domain of the human IFN gamma receptor alpha chain ( IFN gamma R ) fused to a human IgG 1 Fc region ( encoding hinge , CH 2 and CH 3 domain ) that was efficiently expressed as a covalently linked homodimer in insect cells and purified in a one step purification procedure . ^^^ The IFN gamma RFc fusion protein exerted a 3 fold higher ligand binding affinity in binding competition studies in vitro compared with the monovalent extracellular IFN gamma R domain . ^^^ The described IFN gamma R immunoadhesin is a potential therapeutic reagent to interfere with the disease promoting activities of IFN gamma in several autoimmune diseases . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
The absence of IFN gamma function in mice with a targeted disruption of the IFN gamma R alpha chain gene ( IFN gamma R / ) resulted in increased susceptibility to coronaviral hepatitis associated with augmented viral replication and increased hepatocellular injury . ^^^ Unlike MHV infected wild type mice , however , the mutant IFN gamma R / mice showed no increase in IL 12 p4O gene expression , similar to that in naive animals . ^^^ IL 12 treatment failed to restore host resistance in IFN gamma R / mice , but significantly protected MHV susceptible C57BL / 6 mice against lethal infection , although less than IFN gamma treatment . ^^^ Our data demonstrate that despite reduced IL 12 gene expression and defective IFN gamma R function , virus induced IFN gamma production can occur . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
To find out how physiologically secreted IFN gamma controls either the proliferation or the apoptosis of human T lymphocytes , the kinetics of expression of the alpha and beta chains of its receptor ( IFN gamma R ) were sequentially followed on T lymphocytes first activated with PHA and then cultured in the presence of IL 2 , and related to the kinetics of expression of Fas , Bcl 2 , and IL 2R p 55 chain . ^^^ Both IFN gamma R chains were poorly expressed on the membrane of resting T lymphocytes . ^^^ Following their stimulation with PHA , IFN gamma R alpha but not IFN gamma R beta chain up modulated before T lymphocyte entry into the S phase , and then IFN gamma R alpha down modulated when they passed through the S and G2 / M . ^^^ The ensuing proliferative response was inhibited by an anti IFN gamma R alpha mAb that impeded the binding of IFN gamma . ^^^ When PHA activated T lymphoblasts were cultured for 16 days with IL 2 , IFN gamma R alpha expression increased , whereas that of the beta chain remained barely detectable . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : MCA 101 murine tumor cells were transfected with a plasmid expression vector containing an antisense construct to the IFN gamma receptor ( IFN gamma R ) or a control construct . ^^^ Finally , in vivo experiments demonstrated significantly increased untransfected tumor growth rate in animals after blockade of endogenous IFN gamma by a single dose of anti IFN gamma antibody and more rapid growth of the IFN gamma R deficient cells compared to controls . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
In addition , it was shown that mice with disrupted IFN gamma R genes died rapidly after infection with the BCG strain of M . bovis , whereas normal mice survived the infection . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
A complex of human interferon gamma ( IFN gamma ) with the soluble extracellular domain of the IFN gamma receptor alpha chain ( IFN gamma R ) has been crystallised . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Mice with a targeted deletion of either the interferon ( IFN ) gamma gene or the IFN gamma receptor gene ( IFN gamma R ( 0 / 0 ) mice ) fail to survive infection with the Bacillus Calmette Guerin ( BCG ) strain of Mycobacterium bovis . ^^^ Here we show that resident peritoneal macrophages isolated 2 weeks after BCG infection from IFN gamma R ( 0 / 0 ) mice produced significantly less nitric oxide ( NO ) than wild type macrophages . ^^^ However , the response to lipopolysaccharide ( LPS ) was not completely abrogated in the IFN gamma R ( 0 / 0 ) macrophages . ^^^ This increase in urinary nitrite / nitrate was not detected in BCG infected IFN gamma R ( 0 / 0 ) mice , indicating that no other cytokine can replace IFN gamma as a mediator of increased NO synthesis after BCG infection in the intact organism . ^^^ A comparison of circulating levels of IFN gamma in BCG infected animals revealed that sera from IFN gamma R ( 0 / 0 ) mice contained up to 66 fold more IFN gamma than sera from identically treated wild type mice . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
In contrast to the highly species specific cellular homologues , the vaccinia IFN gamma R has novel broad species specificity . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
A systemic lupus like disease was induced by injection of 50 micrograms bacterial LPS twice a week for 4 weeks in wild type and in IFN gamma receptor deficient ( IFN gamma R / ) mice . ^^^ All those alterations took place in a similar manner in wild type and in IFN gamma R / mice . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
A soluble interferon gamma receptor ( IFN gamma R ) is produced early after infection and efficiently blocks the binding of IFN gamma to cellular receptors , thus inhibiting both the anti viral and immune functions of IFN gamma . ^^^ An IFN gamma R is highly conserved among members of the poxvirus family , suggesting a major role in viral pathogenesis . ^^^ The IFN gamma R encoded by myxoma virus , a natural pathogen of rabbits , is specific for rabbit IFN gamma . ^^^ However , the IFN gamma R encoded by orthopoxviruses ( vaccinia , cowpox , camelpox , ectromelia ) shows a novel , broad species specificity suggesting that these viruses have evolved in several species . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
However , the antiviral state induced by transfection with IFN gamma KDEL was strictly dependent on the presence of the IFN gammaR , since fibroblasts derived from IFN gammaR deficient mice ( IFN gammaR / ) were not rendered virus resistant . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
In the present study , we investigated characteristic synthesis of interferon gamma ( IFN gamma ) and expression of IFN gamma receptor ( IFN gamma R ) in the peripheral blood mononuclear cells ( PBMC ) from patients with IgAN and other chronic proliferative glomerulonephritis ( PGN ) . ^^^ Other parts of PBMC pellets were reacted with anti human IFN gamma R monoclonal antibody and FITC labeled anti mouse second antibody for analysis of IFN gamma R expression on these cells by FACScan . ^^^ The isolated cells were evaluated for IFN gamma or IFN gamma R mRNA expression by the semiquantitative RT PCR method . ^^^ On the other hand , the expression of IFN gamma R on macrophages was suppressed in CGN patients . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Here we show that IL 4 is produced during listeriosis by IFN gamma receptor gene disruption ( IFN gammaR ( / ) ) mutant mice . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Studies of hamster human and mouse human somatic fibroblast hybrids and transfected mouse fibroblasts have demonstrated that signaling through the human interferon gamma receptor ( hu IFN gammaR ) requires the formation of a complex consisting of ligand ( IFN gamma ) , a ligand binding receptor chain ( IFN gammaR 1 ) , and a signal transducing receptor chain ( IFN gammaR 2 ) . ^^^ These results demonstrate that while co expression of hu IFN gammaR 1 and hu IFN gammaR 2 is necessary and sufficient for most IFN gamma induced responses , it is not sufficient to confer a generalized antiviral state . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
IL 12 was administered to wild type or IFN gamma receptor deficient ( IFN gamma R / ) mice . ^^^ IL 12 treatment resulted in lower peripheral blood counts and a twofold decrease in bone marrow cellularity and hematopoietic progenitors in wild type mice , but not in IFN gamma R / mice . ^^^ Splenic weight and cellularity were dramatically increased after IL 12 administration in wild type mice and somewhat less in IFN gamma R / mice . ^^^ The increase was predominantly due to NK cell and macrophage infiltration in wild type mice , and strong extramedullary hematopoiesis in IFN gamma R / mice . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
In contrast , activation of T cells , recruitment of immune cells to inflammatory foci , and anti T . gondii IgM antibody production were unaffected by the mutation of the IFN gamma R . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Furthermore , the cytoplasmic domains of the IFN gamma R 2 subunits , like the cytoplasmic domains of the IFN gamma R 1 chains , can be interchanged between species with no loss of biologic activity , demonstrating that the species specific interaction of the IFN gamma R 1 and IFN gamma R 2 chains involves only the extracellular domains of the two proteins . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
We examined the expression of MHC class 1 and 2 in the arterial endothelium of interferon gamma ( IFN gamma , GKO ) and IFN gamma R ( IFN gamma R , GRKO ) gene knockout mice in comparison with mice with intact IFN gamma and IFN gamma R genes , BALB / c and 129Sv / J wild type , respectively . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Another study had suggested that TDRF upregulated both TNF alpha receptor ( TNF alpha R ) and IFN gamma receptor ( IFN gamma R ) mRNA synthesis , as well as increased TNF alpha and IFN gamma binding to their receptors . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
IFN gamma and PRL both affected the expression of the IFN gamma receptor ( IFN gamma R ) gene by increasing IFN gamma R mRNA levels . ^^^ The rise in IFN gamma R transcripts was accompanied by a low but significant release of IL 6 which has previously been shown to stabilize IFN gamma R mRNA . ^^^ Moreover , a transient increase in surface expression of IFN gamma R was observed in U 937 cells treated by IFN gamma alone or in combination with PRL , whereas no apparent modulation of cell surface IFN gamma R was observed in cells treated with PRL . ^^^ Together , our data indicate that IL 6 secretion and increased expression of the IFN gamma R gene correlate with U 937 cell growth arrest induced by IFN gamma and PRL , probably through a signaling mechanism which does not involve the Stat 1 / IRF 1 pathway . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Southern blot and fluorescence in situ hybridization analyses show that NTT is a single copy gene residing in chromosome 6q23 q 24 , near the interferon gamma receptor gene ( IFN gamma R ) . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Binding of interferon gamma ( IFN gamma ) causes oligomerization of the two interferon gamma receptor ( IFN gammaR ) subunits , receptor chain 1 ( IFN gammaR 1 , the ligand binding chain ) and the second chain of the receptor ( IFN gammaR 2 ) , and causes activation of two Jak kinases ( Jak 1 and Jak 2 ) . ^^^ Although IFN gammaR 1 alone can not initiate signal transduction , the chimera EpoR / gammaR1 ( extracellular / intracellular ) generates slight responses characteristic of IFN gamma in response to Epo and the EpoR / gammaR1 . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
IFN gamma has a direct antitumor effect on many tumor cell lines mediated through the IFN gammaR . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
This survival promoting effect of IFN gamma was blocked by neutralizing antibodies to IFN gamma or to IFN gamma receptor ( IFN gamma R ) . ^^^ Polymerase chain reaction ( PCR ) amplification of the alpha chain of IFN gamma R ( IFN gamma R alpha ) yielded products of the correct size predicted from the sequence of the receptor . ^^^ In addition , flow cytometry using anti IFN gamma R monoclonal antibodies ( mAbs ) indicated that these mast cells bear IFN gamma R on their surface . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
To investigate the role of IFN gamma in control of the virus infection , mice lacking the cellular receptor for IFN gamma ( IFN gamma R / mice ) were infected with murine gammaherpesvirus 68 ( MHV 68 ) . ^^^ IFN gamma R / mice showed no difference from wild type mice in the titers of infectious virus in the lungs or in the rate of clearance of the lung infection . ^^^ By 14 days postinfection , spleens from IFN gamma R / mice were pale , shrunken , and fibrous . ^^^ These changes were accompanied by a 10 to 100 fold greater load of latently infected cells in IFN gamma R / mice than in wild type mice at 14 to 17 days postinfection , but this was reduced to the levels found in wild type mice by 21 days postinfection . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
IFN gamma receptor deficient ( IFN gammaR / ) mice were used to study the innate immune responses during infection with Listeria monocytogenes . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Since we have previously shown that IFN gamma exerts a biphasic effect during the evolution of CIA in DBA / 1 mice , we analyzed the development of this disease in mice with a disruption of the IFN gamma receptor gene ( IFN gammaR ( 0 / 0 ) ) . ^^^ Taken together , our findings provide evidence that IFN gammaR deficiency in DBA / 1 mice leads to the occurrence of severe CIA with an accelerated onset compared with that in wild type mice , indicating that the proinflammatory action of IFN gamma has been bypassed in the IFN gammaR ( 0 / 0 ) mice . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Mice with a genetically engineered deficiency for either IL 4 or IFN gamma R 1 ( single mutants ) , and IL 4 / IFN gamma R 1 ( double mutants ) on the Balb / c and 129Sv background were used to study the course of infection with Leishmania major . ^^^ In contrast to genetically resistant 129Sv wildtype mice , IL 4 / IFN gamma R 1 double mutant mice developed fetal disease with parasite dissemination to visceral organs similar to mice lacking IFN gamma R 1 only . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
For this reason , cytokine receptor expression by placental and uterine cells using immunohistology and flow cytometry has been investigated , those being : IGF 1r , PDGF r alpha / beta , IL 1rII , IL 6r , IL 7r , IFN gamma r , TNF rp 80 and endoglin . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
We have previously shown that the contrasting ability of interferon gamma ( IFN gamma ) either to stimulate the proliferation of malignant T cells or to induce their apoptosis is determined by the low and high intensity of IFN gamma receptor ( IFN gamma R ) expression , respectively . ^^^ High IFN gamma R expression is a marker for the T cell stress that precedes apoptosis . ^^^ In this paper , we show that a 12 to 24 h culture of three human malignant T cell lines displaying distinct differentiation stages ( ST 4 , PF 382 , and Jurkat ) in medium supplemented with four chemotherapy drugs ( etoposide , cisplatin , cytarabine , and daunomycin ) up modulates their IFN gamma R expression followed by their apoptosis after 24 48 h later . ^^^ Increased IFN gamma R expression ( by at least an order of magnitude ) was observed in 30 to 90 % of cells during exposure to pharmacologic drug concentrations . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Interferon gamma receptor ( IFN gamma R ) immunoreactivity was observed in the superficial dorsal horn and lateral spinal nucleus in rat and mouse spinal cord . ^^^ IFN gamma R distribution overlapped with nitric oxide synthase 1 immunoreactivity . ^^^ In wild type mice , intrathecal injections of mouse IFN gamma evoked biting behaviour , whereas mice with disruption of the functional gene for IFN gamma R did not respond . ^^^ In contrast to wild type mice , IFN gamma R knock out mice did not show autotomy after sciatic nerve section . ^^^ This study demonstrates a functional IFN gamma R in spinal nociceptive pathways related to neuropathic pain . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Here , we report that human Th 0 and Th 2 clones constitutively express transcripts for the IFN gammaR beta chain , whereas mRNA for this signaling component of the IFN gamma receptor is absent in Th 1 clones . ^^^ The loss of IFN gammaR beta chain expression in Th 1 populations was accompanied by a failure of IFN gamma to induce the expression of the IFN gamma inducible gene , IFN response factor 1 , whereas IFN gamma was effective in inducing IFN response factor 1 mRNA expression in Th 0 and Th 2 cells . ^^^ These results indicate that down regulation of the IFN gammaR beta chain correlates with impaired IFN gamma induced signaling in Th 1 cells . ^^^ Finally , Th 2 populations , generated in the presence of both IL 4 and IFN gamma , expressed levels of IFN gammaR beta chain transcripts similar to those produced by cells differentiated in the presence of IL 4 only , demonstrating that IFN gamma does not modulate the expression of its receptor . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
In addition , both types of clones overexpressed MHC class 1 glycoproteins following IFN gammaR triggering by exogenous IFN gamma , although the kinetics was slower in Th 1 , and this exposure induced mRNA for IRF 1 . ^^^ Apoptosis of both types of clones was mediated by autocrine or exogenous IFN gamma through the up regulation of Fas L expression , since anti IFN gammaR alpha mAb inhibited its expression on Th 1 and exogenous IFN gamma increased its expression on Th 2 . ^^^ These results indicate that activated human Th 1 and Th 2 lymphocytes express IFN gammaR alpha and beta chains and are both sensitive to signals provided by IFN gamma . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Here , we describe experiments showing that IFN gamma receptor knockout ( IFN gammaR alpha KO ) mice of the DBA / 1 strain develop CIA more readily than their wild type counterparts . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
DDC strongly expressed interferon gamma receptor ( IFN gamma R ) ( mAb GIR 208 ) and were negative for IL 8R ( mAb B G 20 ; and B F 25 ) . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
However , IFN gamma activity was not necessary for ICAM 1 induction , as its levels were also increased during infection in IFN gamma receptor knock out ( IFN gamma R ) mice . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
The role of IFN gamma in controlling primary infections with C . pseudotuberculosis was examined in mice deficient for the IFN gamma receptor ( IFN gammaR ( / ) mice ) . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Expression of genes encoding cytokines ( TNF alpha / beta , IL 1 alpha / beta , IL 2 , IL 3 , IL 4 , IL 5 , IL 6 and IFN gamma ) , cytokine receptors ( TNF Rp 55 and p 75 , IL 1R p 60 and p 80 , IFN gamma R , and IL 6R ) , the cell adhesion molecule ( ICAM 1 ) , inducible nitric oxide synthetase ( iNOS ) , anti chymotrypsin ( EB22 / 5 . 3 ) , and the gliotic marker ( GFAP ) was evaluated over a 6 month period using a sensitive RNase protection assay ( RPA ) . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
To determine whether cytokines induced the expression , we infected mice lacking receptors for interferon alpha / beta ( IFN alpha / beta R ( / ) ) , interferon gamma ( IFN gamma R ( / ) ) , and tumor necrosis factor alpha ( TNFRp 55 ( / ) ) . ^^^ TMEV infected IFN gamma R ( / ) and TN FRp 55 ( / ) mice expressed Class 1 antigens in the CNS , whereas IFN alpha / beta R ( / ) mice did not , establishing that IFN alpha / beta mediated the expression . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
To clarify the precise functional roles of IFN gammaR in human CD34+ cells , we examined the effect of IFN gamma alone and in combination with various cytokines on the growth of haemopoietic progenitor cells in CD34+ cells using a serum free clonal culture . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Interferon gamma receptor ( IFN gamma R ) deficient mice parasitized with blood stage Plasmodium chabaudi chabaudi were used to assess the anti malarial activity of interferon gamma ( IFN gamma ) . ^^^ However , IFN gamma R deficient mice displayed an increased leucocytosis and a high mortality rate , whereas all of the wild type mice survived . ^^^ IFN gamma R deficient mice , unlike wild type mice , developed a pronounced second parasitaemia peak , 9 to 11 days after the first one , with a parasitaemia of up to 65 % associated with mortality . ^^^ Furthermore , increased serum levels of nitric oxide ( NO ) were only found in wild type mice at the peak of parasitaemia , whereas it remained at background levels in IFN gamma R deficient mice . ^^^ Parasite specific antibody production was not significantly different in IFN gamma R deficient mice , as compared to wild type mice . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) receptor knock out ( IFN gamma R / ) mice were used to analyse the role of IFN gamma in mucosal immune responses following oral immunization . ^^^ We found that the IFN gamma R / mice demonstrated 50 % reduced spot forming cell ( SFC ) responses in the gut lamina propria and spleen after oral immunization with keyhold limpet haemocyanin ( KLH ) plus cholera toxin ( CT ) adjuvant . ^^^ The IFN gamma R / mice exhibited 10 fold reduced total serum KLH specific antibody levels compared with wild type mice after oral immunization , while after intravenous immunization , no such difference was seen , suggesting a selective impairment of mucosal immune responses . ^^^ Moreover , oral immunizations resulted in impaired interleukin 4 ( IL 4 ) , IL 10 and IFN gamma production by spleen T cells from IFN gamma R / mice , indicating that no reciprocal up regulation of Th 2 activities had occurred despite the lack of IFN gamma R function . ^^^ Despite potentially strong modulating effects of IFN gamma on epithelial cell IgA transcytosis and electrolyte barrier functions , CT immunized IFN gamma R / mice demonstrated unaltered protection against CT in ligated intestinal loops together with normal anti CT IgA and total IgA levels in gut lavage . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Repeated daily exposure of actively immunized mice to aerosolized ovalbumin ( OVA ) , as compared with aerosolized saline ( SAL ) , induced a significant increase in bronchoalveolar lavage fluid ( BALF ) eosinophilia and OVA specific serum IgE in both IFN gamma receptor deficient ( IFN gammaR KO ) and wild type mice . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Further , to characterize IFN gammaR on eosinophils , we did binding experiments with 125I IFN gamma on purified peripheral eosinophils . ^^^ To determine whether IFN gammaR on eosinophils is functional , we examined surface eosinophilic cationic protein ( ECP ) and CD 69 induction after IFN gammaR ligation with recombinant human IFN gamma ( rhIFN gamma ) on eosinophils by flow cytometry . rhIFN gamma stimulation significantly induced both ECP and CD 69 expression on the 2 18 h cultured eosinophils in a dose dependent manner . ^^^ Further , the effects of rhIFN gamma stimulation were significantly blocked by both a neutralizing anti IFN gamma MoAb and a blocking anti IFN gammaR MoAb . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Mice deficient in either IFN gamma , IFN gamma R , IFN alpha / beta R , TNFRs , CD 40 or IL 6 were , in general , highly susceptible to poxvirus infection . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Mutant mice deficient in major histocompatibility complex class 2 molecules ( A beta ( / ) ) , T cell receptor alphabeta cells ( TCR beta ( / ) ) , or all mature T and B lymphocytes ( RAG 1 ( / ) ) were naturally susceptible to P . carinii , whereas mouse mutants lacking the gamma interferon ( IFN gamma ) receptor ( IFN gamma R ( / ) ) or tumor necrosis factor alpha ( TNF alpha ) type 1 receptor ( p 55 ) ( TNF alpha RI ( / ) ) resisted disease acquisition . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
To define the role of IFN gamma in the pathogenesis of glomerulonephritis , we established a population of BW mice deficient in IFN gammaR ( BWgammaR [ / ] ) by repeated crossing ; these mice were compared with BWgammaR ( + / + ) and + / littermates . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Soluble IFN gamma R and IFN levels were evaluated at day 10 ( preconditioning ) , day 0 ( day of BMT ) , day of engraftment , and during BMT related complications using sIFN gamma R specific monoclonal antibodies ( McAB ) followed by double sandwich ELISA , and a sensitive radioimmunoassay respectively . ^^^ Soluble IFN gamma R levels increased in direct correlation with engraftment ( 0 . 63 + / 0 . 11 ng / ml at the day of BMT to 1 . 43 + / 0 . 16 ng / ml at the day of engraftment ; n = 14 ; P < 0 . 001 ) . ^^^ In short , both s IFN gamma R and IFN gamma were found to be significantly elevated during engraftment and GVHD . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
To explore the respective roles of TNF and interferon ( IFN ) gamma in resistance to BCG and granuloma differentiation , controls and sTNFR 1 transgenic mice were compared to IFN gammaR mutant mice and mice double defective in TNF and IFN gamma activity ( obtained by crossing transgenic and mutant mice ) . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
To investigate the role of interferon gamma ( IFN gamma ) in experimental autoimmune thyroidits ( EAT ) , H 2k mice with a disrupted IFN gamma receptor ( IFN gamma R ) gene were immunized with porcine thyroglobulin ( pTg ) . ^^^ We observed that EAT occurred on day 19 and remitted on day 35 in IFN gamma R deficient ( IFN gamma R ( 0 / 0 ) ) mice , whereas in wild type mice , EAT occurred on day 21 and remitted on day 42 49 . ^^^ In contrast , Tg specific IgG 1 was increased in the IFN gamma R ( 0 / 0 ) mice . ^^^ In supernatants from T cells further stimulated in vitro by Tg , IFN gamma levels were higher in IFN gamma R ( 0 / 0 ) than in wild type mice throughout the course of the disease , whereas interleukin 10 was transiently increased prior to EAT onset in both groups of mice . ^^^ Finally , using IFN gamma R ( 0 / 0 ) mice , we demonstrate that induction of EAT does not require an intact IFN gamma system , while progression to full blown disease depends on the action of IFN gamma . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
These responses were analyzed by comparing mouse lines deficient for the gamma interferon ( IFN gamma ) receptor ( IFN gammaR ( / ) ) or deficient for inducible nitric oxide synthase ( iNOS ( / ) ) . ^^^ Despite this defect , both mutant mouse strains had a rather normal proinflammatory cytokine response ( interleukin 12 [ IL 12 ] , IFN gamma , IL 6 ) , with the exception of an impaired tumor necrosis factor alpha and IL 1alpha response in IFN gammaR ( / ) mice , demonstrating that only the latter two cytokines are dependent on IFN gamma activation . ^^^ Moreover , polarization of T cells in type 1 and type 2 T helper ( Th1 / Th2 ) and cytotoxic T ( Tc1 / Tc2 ) cells as well as T . cruzi specific antibody responses were normal in IFN gammaR ( / ) mice , demonstrating that IFN gamma is not necessary for the promotion of T cell differentiation and T . cruzi specific antibody responses . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Chromatography of human ( hu ) plasma from rheumatoid arthritis ( RA ) patients and controls on immobilized hu IFN gamma or antibodies against IFN gamma R alpha chain permitted us to isolate the sIFN gamma R . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
In contrast , MHV infected IFN gamma receptor deficient ( IFN gammaR [ / ] ) mice showed an increased susceptibility to coronaviral hepatitis . ^^^ The virus induced type 1 cytokine secretion pattern was not reversed in IL 12 deficient mice by in vivo neutralization of IFN gamma nor in IFN gammaR ( / ) mice receiving IL 12 neutralizing Abs . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
To determine the impact of IFN gamma in Mphi growth factor incited renal injury , we constructed a MRL Fas ( lpr ) IFN gamma receptor ( IFN gammaR ) deficient strain . ^^^ Proliferation of bone marrow Mphi from IFN gammaR intact MRL Fas ( lpr ) costimulated with CSF 1 or GM CSF and IFN gamma was reduced twofold , while the IFN gammaR deficient MRL Fas ( lpr ) bone marrow Mphi remained stable . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Therefore , we wanted to dissect the different mechanisms exerted by IL 12 during Yersinia infections by using different models of Yersinia resistant and susceptible mice , including resistant C57BL / 6 mice , susceptible BALB / c mice , intermediate susceptible wild type 129 / Sv mice , 129 / Sv IFN gamma receptor deficient ( IFN gamma R / ) mice and C57BL / 6 tumor necrosis factor ( TNF ) receptor p 55 chain deficient ( TNFR p 55 / ) mice . ^^^ IFN gamma R / mice turned out to be highly susceptible to infection by Y . enterocolitica compared with IFN gamma R+ / + mice . ^^^ Administration of IL 12 was protective in IFN gamma R+ / + mice but not in IFN gamma R / mice , suggesting that IFN gamma R induced mechanisms are essential for IL 12 induced resistance against yersiniae . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Expression of interferon gamma receptors and interferon gamma induced up regulation of intercellular adhesion molecule 1 in basal cell carcinoma ; decreased expression of IFN gamma R and shedding of ICAM 1 as a means to escape immune surveillance . ^^^ The expression of interferon gamma receptors ( IFN gamma R ) in the biopsies was also investigated . ^^^ The expression of IFN gamma R was significantly decreased on the tumour cells compared with the overlying epidermis . ^^^ The decreased expression of IFN gamma R on the tumour cells and the shedding of ICAM 1 into the peritumoural stroma may be a plausible mechanism by which the tumour cells are protected against an active cell mediated immune response . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
To examine the impact of IFN gamma on renal injury in MRL Fas ( lpr ) mice , we constructed a IFN gamma R deficient strain . ^^^ In MRL Fas ( lpr ) mice lacking IFN gamma R , circulating and intrarenal CSF 1 were absent , TNF alpha was markedly reduced , survival was extended , lymphadenopathy and splenomegaly were prevented , and the kidneys remained protected from destruction . ^^^ Mesangial cells ( MC ) that were signaled through the IFN gamma R induced CSF 1 and TNF alpha in MRL Fas ( lpr ) mice . ^^^ We detected a large number of apoptotic renal parenchymal cells in advanced nephritis and determined that signaling via the IFN gamma R induces apoptosis of tubular epithelial cells ( TEC ) , but not MC . ^^^ In conclusion , IFN gamma R signaling is essential for the initiation ( CSF 1 ) , acceleration ( CSF 1 and TNF alpha ) , and apoptotic destruction of renal parenchymal cells in MRL Fas ( lpr ) autoimmune kidney disease . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Therefore , we employed IFN gamma R deficient mice to study the role of IFN gamma . ^^^ To introduce the CIA susceptibility gene ( H 2q ) , IFN gamma R deficient ( H 2b / b / IFN gamma R / ) mice were mated with DBA / 1 ( H 2q / q / IFN gamma R+ / + ) mice ; next , the F 1 mice were interbred to yield F 2 offspring bearing different combinations of H 2 ( H 2q / q , H 2q / b , and H 2b / b ) and IFN gamma R ( IFN gamma R+ / + , IFN gamma R+ / , and IFN gamma R / ) genes . ^^^ Although the H 2q allele appeared to confer susceptibility to CIA , mice that were homozygous for the IFN gamma R mutation showed a substantially decreased incidence and severity of CIA . ^^^ The CII specific IgG levels of serum samples , which are known to be involved in the development of CIA , were remarkably reduced in IFN gamma R / mice . ^^^ Furthermore , the anti CII IgG2a levels controlled by IFN gamma R were significantly reduced in IFN gamma R / F 2 mice compared with those seen in IFN gamma R+ / + and IFN gamma R+ / mice , although the levels of all IgG subclass Abs examined were lower in IFN gamma R / mice than in IFN gamma R+ / + mice . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Mice lacking the IFN gamma receptor ( IFN gammaR / ) developed and maintained striking chronic aortic inflammation . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
In control in vitro experiments , macrophages from IFN gammaR or TNFR 55 deficient mice , while failing to respond to IFN gamma or TNF alpha , respectively , produced high levels of NO under appropriate stimulation . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
However , IFN gamma receptor knockout ( IFN gammaR KO ) mice of the DBA / 1 strain have been reported to be more susceptible to CIA than corresponding wild type mice , indicating the existence of an IFN gamma mediated protective pathway in this model . ^^^ In vivo stimulation with anti CD 3 antibody in arthritic IFN gammaR KO mice resulted in production of higher levels of circulating IFN gamma , TNF and IL 2 than in corresponding control mice that had not received the arthritis inducing immunization . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Given that the JAK / STAT is the major , but not the only pathway linked to the IFN gammaR , aim of our work was to investigate the signal transduction pathway ( s ) by which IFN gamma exerts its effects on acute replication of HIV in monocytic cells . ^^^ The functional defect of plus clones in response to IFN gamma was correlated to a selective defect of IFN gammaR 2 , but not IFN gammaR 1 , membrane expression . ^^^ These results strongly support the hypothesis that the JAK / STAT pathway is responsible for the antiretroviral effect of IFN gamma , and further provide evidence for a potential second pathway triggered by IFN gamma in the absence of IFN gammaR 2 chain cell surface expression and involving ISGF3gamma . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
RT PCR was used to measure the gene expression of the IFN gamma receptor ( IFNGR 1 ) , as well as the immunomodulator HLA DR and two enzymes of the 2 5 A pathway . ^^^ In epidermal keratinocytes , the induction of IFNGR 1 was upregulated by incubation with IFN gamma , and this increased with the differentiation potential of the culture medium . ^^^ In mucosal keratinocytes , in contrast , IFN gamma failed to induce expression of IFNGR 1 or the other genes . ^^^ The gene expression of the receptor IFNGR 1 appears to be the dominant factor in the sensitivity of other genes to IFN gamma , although there are substantial disparities among them that presumably reflect functional differences . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
In addition , beta GBP up regulates the expression of both the alpha and the beta chains of the IFN gamma R on activated T lymphocyte membrane . ^^^ None of these effects depend on sugar binding : saturating amounts of lactose do not affect the cell cycle block nor IFN gamma R up modulation . ^^^ They also provide the first evidence of up modulation of IFN gamma R expression on T lymphocytes by a negative cell growth regulator . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Here we show that gamma interferon receptor deficient ( IFN gamma R / ) mice mount a T cell response to lymphocytic choriomeningitis virus ( LCMV ) infection but fail to undergo the transient immune deficiency . ^^^ To determine whether this process was mediated by AICD associated with IFN gamma or with Fas Fas ligand interactions , LCMV specific precursor CTL frequencies were examined in LCMV Clone 13 infected IFN gamma R / or lpr ( Fas deficient ) mice . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Abeta also induced expression of IFN gamma receptor ( IFN gammaR ) both in endothelial and smooth muscle cells . ^^^ Moreover , IL 1beta and IFN gamma synergistically increased the Abeta induced expression of CD 40 and IFN gammaR . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Accordingly , we examined the expression of IFN gammaR alpha and beta chains and the association of the two chains after binding of IFN gamma to the receptor . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
To elucidate the role of IFN gamma in antifungal CD4+ Th dependent immunity , 129 / Sv / Ev mice deficient for IFN gamma receptor ( IFN gammaR ( / ) ) were assessed for susceptibility to gastrointestinal or systemic Candida albicans infection and for parameters of innate and adaptive T helper immunity . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
A murine pulmonary infection model using Mycobacterium bovis BCG was used to study the development of Th 1 and Th 2 type responses in mice lacking a functional IFN gamma receptor ( IFN gamma R / ) . ^^^ Strikingly , the IFN gamma R / mice maintained the Th 1 response and developed a profound M . bovis BCG , specific Th 2 type immune response characterized by IL 5 producing CD4+ T cells , eosinophil infiltration of granulomas , and significantly elevated serum IgE levels . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
These results describe the development of a new synthetic agonist of IFN gamma , which substitutes for the native cytokine in any IFN gamma responsive cells , by acting intracellularly on IFN gammaR . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
We hypothesized that the n 3 PUFA induced hyporesponsiveness to IFN gamma is mediated , in part , by a reduction in the number of IFN gamma receptors ( IFNGR ) expressed on the surface of these cells . ^^^ These data suggest that reduced IFNGR expression may be responsible for immune cell hyporesponsiveness to IFN gamma , which may , in part , explain some of the immunomodulatory and anti inflammatory effects associated with the consumption of diets high in n 3 PUFA . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
The purpose of this study was to investigate whether the amino acid polymorphism ( Val14Met ) found within the IFN gamma receptor gene ( IFNGR 1 ) plays a prominent role in susceptibility to SLE . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
This correlated with reduced expression of IFN gamma R ( CD 119 ) by mature DC . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Mice with intact genes ( wild type ) , and knockout mice with disrupted IFN gamma ( IFN gamma / ) or IFN gammaR ( IFN gammaR / ) genes were studied . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Whereas TGF beta R ( CD 105 ) , MCSFR ( CD 115 ) , SCFR ( CD 117 ) , IL 4R / IL 13R ( CD 124 ) and IL 6R ( CD 126 ) were not expressed , the cell lines were mostly positive for IFN gamma R ( CD 119 ) , IL 7R ( CD 127 ) and FLT 3R ( CD 135 ) . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Tyrosine phosphorylation of JAK 1 , JAK 2 , and IFN gamma R alpha was also reduced in infected cells . ^^^ Northern and Western blot analyses showed that a down regulation of IFN gamma R alpha and beta chain mRNA and protein occurred in M . avium infected macrophages . ^^^ The down regulation of IFN gamma R and inhibition of STAT 1 activation were time dependent and required 4 h of infection for down regulation of the IFN gamma R and 8 h for STAT 1 inhibition . ^^^ These findings suggest that M . avium infection inhibits induction of IFN gamma inducible genes in mouse macrophages by down regulating IFN gamma R , resulting in reduced phosphorylation of IFN gamma R alpha , JAK 1 , JAK 2 , and STAT1 . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
An intense expression of interferon gamma receptor ( IFN gamma R ) was found in the basal layers of the cholesteatoma matrix , and overexpression of the epithelial growth factor receptor ( EGFR ) was found in all layers of the matrix . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
IFN gamma receptor chain 1 ( IFNGR 1 ) tyrosine phosphorylation is markedly enhanced in cells treated with TNF alpha / IFN gamma without alteration in receptor levels . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Specifically , mice deficient for the interferon gamma receptor ( IFN gammaR / ) were used to determine the role for IL 12 in the absence of IFN gamma mediated signalling . ^^^ We show that recombinant IL 12 administered to IFN gammaR / mice caused the up regulation of mRNA for IFN gamma in lung tissue , and the secretion of abundant IFN gamma by in vitro cultured lymph node cells in response to egg antigens . ^^^ We also show that increases in mRNA for the beta 2 subunit of the IL 12 receptor and the p 40 subunit of IL 12 after rIL 12 treatment were lower in IFN gammaR / mice , compared to wild type mice , indicating that their expression was primarily dependent upon IFN gamma with only a minor role for IL 12 . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
At 72 h , cells that evade apoptosis start to proliferate again , overexpress both chains of the IFN gammaR , and thus become susceptible to apoptosis in the presence of IFN gamma . ^^^ By contrast , in the presence of IFN gamma , no apoptosis , but an increase of proliferation was displayed by control cultures of T cells not exposed to NO and not overexpressing IFN gammaR chains . ^^^ The NO induced cell surface overexpression of IFN gammaR chains did not affect the transduction of IFN gamma mediated signals , as shown by the expression of the transcription factor IFN regulatory factor 1 ( IRF 1 ) . ^^^ By inducing the overexpression of IFN gammaR chains , NO decides whether IFN gamma promotes cell proliferation or the induction of apoptosis . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Low surface IFN gamma R 2 expression appears to be due to recycling between cytoplasmic stores and the cell surface , which does not depend on signals mediated by endogenous IFN gamma , because IFN gamma R 2 surface expression is low , and its internalization is equally observed in patients with inherited IFN gamma R 1 gene deficiency and in healthy donors . ^^^ Moreover , IFN gamma R 2 internalization in T lymphoblasts from healthy donors was not affected by the presence of anti IFN gamma neutralizing or anti IFN gamma R 1 blocking mAb . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Flow cytometric analysis revealed that biotinylated IFN gamma bound equally well to purified peritoneal mast cells from both IFN gammaR knockout and wild type mice , indicating a non IFN gammaR binding site . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Cellular responses to IFN gamma are mediated by its heterodimeric cell surface receptor ( IFN gammaR ) , which activates downstream signal transduction cascades , ultimately leading to the regulation of gene expression . ^^^ In order to study the role of IFN gamma in a number of immune responses and pathways , researchers have generated mice with altered patterns of IFN gammaR gene expression . ^^^ These studies , together with analyses of naturally occurring mutations of the IFN gammaR in man , have been instrumental in elucidating the diverse functions of IFN gamma , and are the subject of this review . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
We have now used IFN receptor subunit knockout mice to produce mouse fetuses that carry three No . 16 chromosomes and one copy each of disabled IFN gamma receptor ( IFNGR ) and IFN alpha / beta receptor ( IFNAR 2 ) component genes . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
IFN gamma stimulation induced marked phosphorylation of its own receptor ( IFN gammaR ) alpha chain . ^^^ Inhibition of the IFN gammaR complex formation correlated with inhibition of STAT1alpha binding to the IFN gamma response region . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
These results indicate that the protection of NOD mice from the development of diabetes is not directly linked to the defective IFN gamma receptor gene but , rather , is influenced by the presence of a diabetes resistant gene ( s ) closely linked to the IFN gammaR loci derived from the 129 mouse strain . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Immunoreactivity for the interferon gamma receptor ( IFN gamma R ) occurs in the superficial layers of the dorsal horn and the lateral spinal nucleus in the rat and mouse spinal cord , as well as in subsets of neurons in the dorsal root ganglia . ^^^ The aim of the present study was to examine the cellular localization and origin of the IFN gamma R in the spinal cord . ^^^ As viewed by confocal microscopy , the immunopositivity for the IFN gamma R was co localized with that of the presynaptic marker synaptophysin and with neuronal nitric oxide synthase in the lateral spinal nucleus , whereas only a minor overlap with these molecules was observed in laminae 1 and 2 of the dorsal horn . ^^^ There was no co localization of the IFN gamma R with markers for astrocytes and microglial cells . ^^^ Ultrastructurally , the IFN gamma R was found predominantly in axon terminals in the lateral spinal nucleus , but at postsynaptic sites in dendrites in laminae 1 and 2 . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
The role of the interferon gamma ( IFN gamma ) receptor 1 ( IFN gammaR 1 ) was investigated in the regulation of MHC expression in kidney in the basal state , in response to potent inflammatory stimuli , and after renal injury . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
We now report that differently from sporozoite induced immunity , interferon ( IFN ) gamma does not mediate the protective immunity induced by AdPyCS , since a similar degree of protection was observed in AdPyCS immunized mice lacking IFN gamma / and the IFN gamma receptor ( IFN gammaR / ) compared to that in wild type mice . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
The cerebrum and cerebellum were collected from young adult ( 8 10 weeks old ) and aged ( 24 26 months old ) BALB / c mice , and the expressions of IFN gamma and IFN gamma receptor 1 ( IFNGR 1 ) mRNA were examined by RT PCR . ^^^ Expression of IFN gamma and IFNGR 1 was also identified in isolated microvessels from brains . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Furthermore , spleen cells isolated from IFN gamma receptor ( IFN gammaR ) deficient mice secreted IL 5 upon allogeneic stimulation in primary MLC . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Internalization and nuclear translocation of IFN gamma and IFN gammaR : an ultrastructural approach . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
IFN gamma displays several effects on different tissues via its specific cell surface receptor ( IFN gammaR ) . ^^^ In order to identify the target compartments of IFN gamma and IFN gammaR ( alpha and beta chains ) , we used a quantitative immunogold approach . ^^^ In physiological conditions , IFN gamma and IFN gammaR immunoreactivities were detected in the plasma membrane , in the endoplasmic reticulum area , in the mitochondria and in the nucleus . ^^^ After a single IFN gamma injection , we observed , in a quantitative manner , an increase of signal density without modification of the subcellular distribution of IFN gamma and IFN gammaR subunits . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
The quantitative expression of genes for CD 14 , interferon gamma receptor ( IFN gamma R ) , tumor necrosis factor alpha ( TNF alpha ) , integrin alpha 4 beta 1 ( VLA 4 ) , tumor necrosis factor receptor p 55 ( TNFR 1 ) and CD 44 ( standard form ) was examined in 9 patients with primary or secondary leg lymphedema before and after phase 1 of CDP . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Constitutive expression of the interferon gamma receptor protein ( IFN gammaR ) , and the distribution of cells in which Fos , a marker of cell activation , is induced by intracerebroventricular administration of IFN gamma , were studied in the rat brain by immunohistochemistry . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
By using mice genomically lacking IFN gammaR , IL 12 , perforin , and recombination activating gene 1 ( RAG 1 ) , we analyzed the regulation and importance of IFN gamma in the control of infection with Chlamydia pneumoniae . ^^^ In experiments including RAG 1 / / IFN gammaR / mice we showed that IFN gamma produced by innate cells controls the bacterial load and is necessary for the increased accumulation of transcripts for enzymes controlling high output NO release ( inducible NO synthase ) , superoxide production ( gp 91 NADPH oxidase ) , and catalysis of tryptophan ( indoleamine 2 , 3 dioxygenase ( IDO ) ) , mechanisms probably related to bacterial killing . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Importantly , deletion of IFN gamma or IFN gammaR in several lupus predisposed mouse strains resulted in significant disease reduction , suggesting the potential for therapeutic intervention . ^^^ Therapy significantly reduced serum levels of IFN gamma , as well as disease manifestations ( autoantibodies , lymphoid hyperplasia , glomerulonephritis , mortality ) , when treatment was initiated at the predisease stage , particularly when IFN gammaR / Fc expression was enhanced by electroporation at the injection site . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
DBA / 1 mice deficient in expressing the interferon gamma ( IFN gamma ) membrane receptor ( IFN gammaR KO mice ) are more susceptible to collagen induced arthritis ( CIA ) than wild type mice , indicating that endogenous IFN gamma plays a protective role in the pathogenesis of CIA . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Experiments performed in IFN gammaR knockout mice demonstrated that IFN gamma was not involved in the killer activity of IL 12 / IL 18 derived NK cells . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
A mutant form of human interferon gamma ( IFN gamma SC 1 ) that binds one IFN gamma receptor alpha chain ( IFN gamma R alpha ) has been designed and characterized . ^^^ Isothermal titration calorimetry shows that IFN gamma SC 1 forms a 1 : 1 complex with its high affinity receptor ( IFN gamma R alpha ) with an affinity of 27 ( + / 9 ) nM . ^^^ As a result , surface charge rather than structural changes is likely responsible for the inability of the His 111 > Asp domain of to bind IFN gamma R alpha . ^^^ The AB loops of IFN gamma SC 1 adopt conformations similar to the ordered loops of IFN gamma observed in the crystal structure of the IFN gamma / IFN gamma R alpha complex . ^^^ Thus , IFN gamma R alpha binding does not result in a large conformational change in the AB loop as previously suggested . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
On day 14 of culture , cells constitutively expressed CD 69 and the IFN gamma receptor ( IFN gammaR ) . ^^^ Stimulation with IFN gamma for 24 h did not affect IFN gammaR expression by the cells . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Differential nuclear localization of the IFNGR 1 and IFNGR 2 subunits of the IFN gamma receptor complex following activation by IFN gamma . ^^^ Because the uptake of IFN gamma is a receptor mediated endocytotic process , we examined in this study the fate of both the receptor subunits ( IFNGR 1 and IFNGR 2 ) of the heterodimeric IFN gamma receptor complex . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
We show that this is IFN gamma dependent but does not require IFN gamma receptor ( IFN gamma R ) expression on tumor cells , T cells , or other hematopoietic cells and that IFN gamma R expression is not necessary in the priming phase . ^^^ However , tumor immunity requires IFN gamma R expression on nonhematopoietic cells in the effector phase and involves inhibition of tumor induced angiogenesis . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Splenocytes from mf infected animals produce high levels of gamma interferon ( IFN gamma ) upon in vitro restimulation with Ag , and experiments in IFN gamma receptor deficient ( IFN gamma R ( / ) ) mice demonstrated that signaling via the IFN gamma R is essential in the induction of NO production and subsequent proliferative suppression . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
We have previously shown that infection of gamma interferon receptor knockout ( IFN gamma R ( / ) ) mice with gammaherpesvirus 68 ( gamma HV 68 ) results in severe inflammation of the large elastic arteries that is pathologically similar to the lesions observed in Takayasu ' s arteritis , the nongranulomatous variant of temporal arteritis , and Kawasaki ' s disease ( K . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
IFN gamma exerts its pleiotrophic effects by transcriptional regulation of expression of numerous genes , such as major histocompatibility complex ( MHC ) class 1 and Fas , through interaction with IFN gamma receptor ( IFN gamma R ) . ^^^ Although hepatocytes in normal liver express weak or no IFN gamma R , those in acute and chronic liver disease up regulate its expression . ^^^ A study using IFN gamma R alpha chain knock out mice revealed the actions of IFN gamma on tumor cells as an extrinsic tumor suppressor mechanism . ^^^ We examined the expression of IFN gamma R and IFN gamma inducible genes in 44 cases with HCC using real time reverse transcriptase polymerase chain reaction ( RT PCR ) and immunohistochemistry . ^^^ In noncancerous liver tissues ( n = 38 ) , IFN gamma R expression on the cell surface was up regulated in 27 cases . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Along with SLAM , expression of the IL 12 receptor chain beta 2 ( IL 12R beta 2 ) and the IFN gamma receptor chain beta ( IFN gamma R beta ) proved to be useful molecular markers indicating the state of T cell polarisation , as previously reported . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
For example , Th 2 cells express both chains of the interferon gamma receptor ( IFN gammaR ) , whereas Th 1 cells do not express the second chain of the IFN gammaR ( IFN gammaR 2 ) and are therefore unresponsive to IFN gamma . ^^^ To determine whether the regulation of IFN gammaR 2 expression , and therefore IFN gamma responsiveness , is important for the differentiation of naive CD 4 ( + ) T cells into Th 1 cells or for Th 1 effector function , we generated mice in which transgenic ( TG ) expression of IFN gammaR 2 is controlled by the CD 2 promoter and enhancer . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
For this purpose , we used various knockout mice lacking molecules , which include interferon gamma ( IFN gamma ) , interferon gamma receptor ( IFN gamma R ) , interferon regulatory factor 1 ( IRF 1 ) , inducible nitric oxide synthase ( iNOS ) , and perforin . ^^^ When we infected these mice with MCMV and determined the viral titers in their lungs at different time points , we found that IFN gamma , IFN gamma R , IRF 1 , iNOS , and perforin deficient mice developed significantly higher titers of infectious MCMV in the lung , compared to those observed in their respective wild type controls . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Systemic production and mobilization of inflammatory cells and formation of hepatic periovular granulomas were studied in Schistosoma mansoni infected mice with deficient interferon gamma ( IFN gamma ) receptor ( IFN gammaR ( o / o ) ) . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Mutations in IFNGR 1 and IFNGR 2 , encoding the two IFN gamma receptor chains , and mutations in STAT 1 , encoding an essential signaling component , result in impaired cellular responses to IFN gamma . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Wild type 129 Sv Ev mice ( 129 mice ) and IFN gamma receptor knockout mice based on the 129 background ( IFN gammaR ( ) ) were challenged orally with approximately 5 . 5 10 10 ( 7 ) L . intracellularis cells . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
CD 28 ligation induced not only IL 12Rbeta1 and beta 2 mRNA expression , but also enhanced IFN gammaR to mediate up regulation of IL 12R by IFN gamma . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
This gene is not located in the 5q31 q 33 region , but maps to chromosome 6q22 q 23 and is closely linked to the IFN gamma R 1 gene encoding the receptor of the strongly anti fibrogenic cytokine Interferon gamma . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Recently , recessive mutations in the interferon gamma receptor ligand binding chain ( IFN gamma R 1 ) , interferon gamma receptor signalling chain ( IFN gamma R 2 ) , interleukin 12 p 40 subunit ( IL 12 p 40 ) , and interleukin 12 receptor beta 1 chain ( IL 12R beta 1 ) genes have been identified in a number of patients with disseminated BCG or NTM infection . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
CD 40 modulation of IFN gamma action on lymphoid DC involved a reduction in IFN gamma R expression and tryptophan degrading ability . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
To study the role of tumour necrosis factor alpha ( TNF alpha ) on some of these metabolic responses to endotoxin , we have used transgenic mice expressing soluble tumour necrosis factor receptor 1 IgG fusion protein ( TNFR 1 IgG ) as well as TNF alpha knockout ( KO ) , lymphotoxin alpha ( LT alpha ) KO , and interferon gamma receptor ( IFN gamma R ) KO mice . ^^^ The anorectic response shows the strongest association with IFN gamma since both IFN gamma R KO mice and mice treated with anti IFN gamma antibody showed marked reduction in the LPS induced anorexia compared to other mice . ^^^ IFN gamma R KO mice also have an attenuated thermogenic response to endotoxin . ^^^ Anti Asialo GM 1 antibody treatment attenuated both the hypermetabolic and anorectic responses to LPS , to an extent comparable to that observed in IFN gamma R KO mice . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
METHODS AND RESULTS : We showed that IFN gamma receptor deficient mice ( IFN gammaR ( / ) ) on a BALB / c background immunized with a peptide derived from cardiac alpha myosin heavy chain develop severe myocarditis with high mortality . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
The IFNGR 1 signal transducing subunit of the IFN gamma receptor is tyrosine phosphorylated through the chimeric receptors and the endogenous IL 6 and OSM receptors . ^^^ Cross phosphorylation of IFNGR 1 is not , however , required for the IFN gamma like response through the chimeric receptors , nor does it mediate an IFN gamma like response to IL 6 or OSM . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Recently , mutations in the interferon gamma receptor ligand binding chain ( IFN gamma R 1 ) , interferon gamma receptor signaling chain ( IFN gamma R 2 ) , Signal Transducer and Activator of Transcription 1 ( STAT 1 ) , interleukin 12 p 40 subunit ( IL 12 p 40 ) , and interleukin 12 receptor beta 1 chain ( IL 12R beta 1 ) genes have been identified in a number of patients with severe BCG or NTM infection . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
PURPOSE : To investigate the mRNA expression of the receptors for tumour necrosis factor alpha ( TNFRp 55 , TNFRp 75 ) , interferon gamma ( IFN gamma R alpha , IFN gamma R beta ) , interleukin 10 ( IL 10R , CRFB 4 ) and transforming growth factor beta ( TGF beta RII ) on human retinal pigment epithelium ( RPE ) cells and to modulate this expression with the pro inflammatory cytokines TNF alpha and IFN gamma as stimulators . ^^^ RESULTS : The mRNA for TNFRp 55 , TNFRp 75 , IFN gamma R alpha , IFN gamma R beta , CRFB 4 and TGF beta RII was constitutively expressed in vitro . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
However , such restimulation of Th 1 primed cells from interferon ( IFN ) gamma ( 2 / ) or IFN gamma receptor ( IFN gammaR ) ( / ) mice resulted in substantial production of IL 4 and other Th 2 cytokines . ^^^ Even IFN gamma producing cells from IFN gammaR ( / ) mice could acquire IL 4 producing capacity . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Reporter gene analyses of 5 ' deletion constructs of the IFN gamma receptor 1 gene ( IFNGR 1 ) promoter indicated that the critical region for control of transcription and the TPA responsive element ( TRE ) were present in the 128 to 109 base pair ( bp ) region . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Using IFN gamma R ( / ) and the respective wild type mice , we demonstrate that although sensitization to LPS and killed Listeria monocytogenes is exclusively IFN gamma dependent , an IFN gamma independent , moderate sensitization to certain TNF alpha inducing constituents in bacteria may develop in parallel . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
After stimulation , these T cells produced high levels of the pro inflammatory cytokine , IFN gamma , and developed an increased population of IFN gamma ( + ) IFN gamma R ( ) T cells . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
It has been hypothesized that thymulin increases the responsiveness of immune cells to IFN gamma by either increasing the expression of IFN gammaR or by increasing the production and / or secretion of IFN gamma . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Analysis of IFN gamma producing CD 8 ( + ) T cells demonstrates that , like Th 1 cells , these cells do not express IFN gammaR 2 . ^^^ To define the importance of IFN gamma signaling for the development of functional CD 8 ( + ) T cells , mice either lacking IFN gammaR 2 or overexpressing this protein were examined . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
We have studied the function of IFN gamma and its receptor in the EAE model using two different IFN gamma receptor knockout ( IFN gamma R ( / ) ) mouse types : C57Bl / 6x129Sv , with a disruption of the IFN gamma receptor cytoplasmic domain , and 129Sv , homozygous for a disrupted IFN gamma receptor gene . ^^^ However , it decreased the mean daily score in IFN gamma R ( / ) 129Sv and the incidence of the disease down to 50 % in C57Bl / 6x129Sv IFN gamma R ( / ) mice . ^^^ Moreover , after anti IFN gamma mAb treatment , oxidative stress levels , metallothionein 1 and 2 antioxidant protein expression , and apoptoticneuronal death were increased in wild type mice while decreased in IFN gamma R ( / ) mice . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Administration of cDNA encoding soluble IFN gamma receptor ( IFN gamma R ) / IgG Fc fusion proteins , soluble TNF alpha receptors , or IL 1 receptor antagonist ( IL 1ra ) , protects against either lupus , various forms of arthritis , autoimmune diabetes , or other autoimmune diseases . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Differential cell membrane expression of IFN gammaR 2 determined the number of receptor complexes that transduced the IFN gamma signal and resulted in a different response to IFN gamma . ^^^ After IFN gamma stimulation , high IFN gammaR 2 membrane expression induced rapid activation of signal transducer and activator of transcription 1 ( STAT 1 ) and high levels of interferon regulatory factor 1 ( IRF 1 ) , which then triggered the apoptotic program . ^^^ Because the forced expression of IFN gammaR 2 on T cells switched their response to IFN gamma from proliferative to apoptotic , we concluded that the surface expression of IFN gammaR 2 determines whether a cell stimulated by IFN gamma undergoes proliferation or apoptosis . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
To address this issue , we measured immune responses in mice with targeted deletions of the ISS induced genes encoding IL 12 ( IL 12 ( / ) ) , IFN gamma ( IFN gamma ( / ) ) , the IFN gamma receptor ( IFN gammaR ( / ) ) , and the IFN alpha / beta receptor ( IFN alpha / betaR ( / ) ) after immunization with ISS containing oligodeoxynucleotides and model antigens . ^^^ IL 12 ( / ) and IFN alpha / betaR ( / ) mice were compromised in their ability to develop a cross primed CTL response , whereas IFN gamma ( / ) and IFN gammaR ( / ) mice were not . ^^^ Antigen nonspecific ISS induced B cell proliferation was normal in the four deficient strains ; however , innate IL 6 production was reduced in IFN gamma ( / ) and IFN gammaR ( / ) splenocytes and eliminated in IFN alpha / betaR ( / ) cells . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Its biological activity depends on expression of IFN gamma receptor ( IFN gammaR ) . ^^^ To address whether increased expression of IFN gammaR is associated in vivo with a higher biological response by IFN gamma , we constructed an adenovirus vector including murine IFN gammaR ( Ad mIFN gammaR ) . ^^^ To our knowledge , this is the first report of adenovirus mediated IFN gammaR gene transfer being effective in augmenting the biological activity of IFN gamma , and the strategy employed in the present study will be useful in studying other kinds of cytokine receptors and applications to gene therapy for cancer and infectious diseases . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Here we demonstrate that the IFN gammaR from 10 , cowpox virus and camelpox virus exists naturally as a homodimer , whereas the cellular IFN gammaR dimerizes only upon binding the homodimeric IFN gamma . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
The survival period of B 16 melanoma bearing IFN gamma R ( / ) mice was , however , shorter than wild type mice . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Phytohemagglutinin ( PHA ) derived T lymphoblasts or T cell clones from patients genetically deficient in IL 12R beta 1 ( IL 12R beta 1 ( / ) ) or IFN gamma R 1 ( IFN gamma R 1 ( / ) ) produced two to threefold reduced IFN gamma levels compared to the corresponding cells from healthy individuals after anti CD 3 and PMA stimulation . ^^^ Moderate IFN gamma production was observed in PHA derived T lymphoblasts or T cell clones derived from healthy subjects in the presence of anti IFN gamma R 1 or anti IL 12 mAb , whereas it was negligible in the presence of both mAb . ^^^ However , when anti IFN gamma R 1 and / or anti IL 12 mAb were added during restimulation , the cells produced normal levels of IFN gamma , indicating that both IFN gamma and IL 12 had an effect on the priming phase . ^^^ Moderate production of IFN gamma was partially enhanced only in IFN gamma R 1 ( / ) T cell clones generated in the presence of IL 12 , but was almost completely abolished when IL 12R beta 1 ( / ) and IFN gamma R 1 ( / ) T cell clones were generated in the presence of anti IFN gamma R 1 or anti IL 12 mAb , respectively . ^^^ IL 4 production was enhanced in T cell clones from IL 12R beta 1 ( / ) , but not from IFN gamma R 1 ( / ) patients , whereas IL 10 and IL 2 production did not differ significantly in polyclonal T cells or clones from healthy and deficient individuals . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
To determine the role of interferon ( IFN ) gamma in pneumonia , IFN gamma receptor deficient ( IFN gamma R ( / ) ) and 129 / Sv ( wild type [ wt ] ) mice were inoculated intranasally with Streptococcus pneumoniae . ^^^ Although mortality did not differ between the groups 48 h after inoculation , IFN gamma R ( / ) mice had significantly fewer pneumococci in their lungs than the wt mice . ^^^ The relatively increased resistance of IFN gamma R ( / ) mice was not related to favorable effects on defense mechanisms known to contribute to antibacterial immunity that is , the neutrophilic influx was reduced and the cytokine and nitric oxide levels were similar or lower in IFN gamma R ( / ) mice . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
METHODS : We investigated the association of polymorphisms in the T helper ( Th ) 1 and Th 2 cytokine , and related genes ( interferon [ IFN ] gamma , IFN gamma receptor 1 [ IFN gamma R 1 ] , IFN gammaR 2 [ IRF 1 ] , interleukin 12 receptor beta 1 [ IL 12Rbeta1 ] , IL 4 , IL 4R , and IL 10 genes ) with SSPE in Japanese subjects . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
This study explored the relationship between polymorphisms in the promoter region of the gene encoding IFN gamma receptor 1 ( IFNGR 1 ) and susceptibility to malaria in African children . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
SOCS 1 and SOCS 3 overexpression in keratinocyte stable clones inhibited IFN gamma induced phosphorylation of IFN gammaR ( alpha ) and activation of STAT 1 and STAT 3 . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
This protein has amino acid similarity with the extracellular domain of cellular IFN gamma receptor ( IFN gammaR ) and binds and inhibits IFN gamma from a wide range of species . ^^^ In mice , the loss of the 10 IFN gammaR did not affect virulence compared with WT and revertant viruses , consistent with the low affinity of the 10 IFN gammaR for mouse IFN gamma . ^^^ Lastly , the affinity constants of the 10 IFN gammaR for human and mouse IFN gamma were determined by surface plasmon resonance . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Since CD 14 plays a key role in LPS induced cell activation the regulation and function of CD 14 in this sensitization process were studied in IFN gamma R / and the respective wild type ( wt ) mice . ^^^ This response was completely absent in IFN gamma R / mice and is therefore strictly IFN gamma dependent . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
In this report , we have characterized the domains of Jak 1 required to associate with distinct cytokine receptor subunits : IFN alpha R beta L , IFN gamma R alpha , IL 10R alpha , IL 2R beta , and IL 4R alpha . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
IFN gamma contains a nuclear localization sequence that may play a role in the nuclear transport of activated STAT1alpha via a complex of IFN gamma / IFN gamma receptor ( IFNGR ) 1 / STAT1alpha with the nuclear importer nucleoprotein interactor 1 . ^^^ However , treatment of cells with IFN gamma resulted in rapid migration of IFNGR 1 , but not IFNGR 2 , from these microdomains . ^^^ Filipin pretreatment , which specifically inhibits endocytosis from caveolae like microdomains , inhibited the nuclear translocation of IFN gamma and IFNGR 1 as well as the tyrosine phosphorylation and nuclear translocation of STAT1alpha , but did not affect the binding of IFN gamma to these cells . ^^^ Isolation of lipid microdomains from Jurkat cells showed that both IFNGR 1 and IFNGR 2 were associated with lipid microdomains only after stimulation with IFN gamma , suggesting that the IFNGR subunits are recruited to lipid microdomains by IFN gamma binding in lymphocytes ( Jurkat ) in contrast to their constitutive presence in epithelial ( WISH ) cells . ^^^ In contrast , treatments that block clathrin dependent endocytosis did not inhibit either activation or nuclear translocation of STAT1alpha or the nuclear translocation of IFN gamma or IFNGR 1 . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Here we describe macrophage and T cell function in eight individuals with partial IFN gamma receptor 1 ( IFN gammaR 1 ) deficiency due to a mutation that results in elevated cell surface expression of a truncated IFN gammaR 1 receptor that lacks the intracellular domain . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Here we analyze the role of IFN gamma during EAE by using both IFN gamma receptor knockout ( IFN gamma R ( / ) ) and wild type mice , both strains immunized with peptide 40 55 from rat myelin oligodendrocyte glycoprotein . ^^^ The levels of oxidative stress , MT I+II , and apoptotic cell death by EAE were significantly increased in all mice , though more so in IFN gamma R ( / ) mice compared with wild type mice . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Therefore , bile duct ligation ( BDL ) was induced in IFN gamma alpha chain receptor deficient ( IFN gammaR ( 1 ) / ) and wild type ( IFN gammaR ( 1 ) + / + ) mice . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
We show that anti CD 3 Ab induces IL 15 mRNA in spleens of wild type but not IFN gamma receptor knock out ( IFN gammaR KO ) mice . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
A multipoint LOD score of 3 . 1 was obtained at IFNGR 1 , the gene that encodes chain 1 of the interferon gamma ( IFN gamma ) receptor . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
OBJECTIVE : To study the possible differences in the interferon gamma receptor ( IFN gamma R 1 ) response among a variety of clinical types in patients with chronic hepatitis B ( CHB ) and implications in pathogenesis . ^^^ METHODS : The serum level of IFN gamma and the expression level of IFN gamma R 1 in peripheral leucocytes , from 53 CHB patients , were examined by ELISA and flow cytometry respectively , which were compared with the baseline levels of 15 healthy controls and were performed correlation analysis with alanine aminotransferase ( ALT ) , total bilirubin ( TBil ) in serum and morphological change in hepatic tissues . ^^^ RESULTS : The results showed that the level of IFN gamma R 1 ( 28 . 89 % 11 . 77 % ) expressed on the membranes of lymphocytes in CHB patients , which correlated with liver inflammation ( r=0 . 621 , P < 0 . 01 ) and serum TBil level ( r=0 . 575 , P < 0 . 01 ) , was much higher than that ( 9 . 23 % 1 . 30 % ) of the healthy controls ( Z=3 . 988 , P < 0 . 05 ) , and no obvious difference on the membranes of leucocytes . ^^^ CONCLUSION : These findings suggest that the IFN gamma signal transduction pathway is modulated through up regulating the expression of IFN gamma R 1 on the membranes of lymphocytes , which takes part in the immuno pathogenesis in CHB patients . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Individuals with mutations in either chain of the IFN gamma receptor ( IFN gammaR ) are susceptible to infection with mycobacteria . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
In contrast , IFN gamma receptor ( IFNGR ) expression was increased in the presence of anti CD 2 mAb . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
We previously showed decreased OPN expression in the granulomas of patients with disseminated mycobacterial infection in the setting of mutation of the IFN gamma receptor 1 ( IFNGR 1 ) . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Both IGF 1 and anti IGF 1R mAb did not modify the constitutive expression of IFN gamma R 2 mRNA in T cells as well as the high IFN gamma R 1 binding chain surface expression in T , B , and myeloid cells . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Endogenous accumulation of IFN gamma in IFN gamma R ( / ) mice increases resistance to B16F10 Nex 2 murine melanoma : a model for direct IFN gamma anti tumor cytotoxicity in vitro and in vivo . ^^^ In contrast , IFN gamma R ( / ) ( GRKO ) mice are remarkably resistant to tumor development . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
M . tuberculosis interferes with IFN gamma receptor ( IFN gamma R ) signaling in macrophages , but the molecules responsible for this inhibition are poorly defined . ^^^ Thus , M . tuberculosis , through 19 kDa lipoprotein activation of TLR 2 , inhibits IFN gamma R signaling in human macrophages , resulting in decreased MHC 2 Ag processing and recognition by MHC 2 restricted CD 4 T cells . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
This enhanced signal transduction may be due to the increase in IFN gamma receptor ( IFNGR ) expression following combined cytokine stimulation and is a likely contributor to the synergy . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Using an intronic ( CA ) n polymorphic microsatellite marker within the IFN gamma receptor 1 ( IFNGR 1 ) gene , we have compared the allelic frequencies of this marker in hospitalized tuberculosis patients ( n = 120 ) with that of controls ( n = 87 ) from Rijeka , Croatia . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Because interleukin ( IL ) 12 and interferon gamma ( IFN gamma ) productions are known to be a hallmark of Th 1 dominant intestinal inflammation such as 2 , 4 , 6 trinitrobenzene sulfonic acid ( TNBS ) induced colitis , we strictly addressed the roles of IFN gamma and IL 12 in the development of colitis , employing knockout mice with IFN gamma receptor ( IFN gammaR ) or IL 12 p 40 gene disruptions and mice administered with neutralizing monoclonal antibodies ( mAbs ) against IFN gamma or IL 12 . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
RTPCR using naive conjunctivas detected more IL 4 , IFN gamma , and IFN gamma R beta chain RNA expression in BN rats . ^^^ After the EC induction , BN rats expressed significantly higher amounts of IFN gamma R beta chain , and upregulation of interferon regulatory factor ( IRF ) 1 was observed . ^^^ The findings demonstrate differential expression of IFN gamma R and signaling through IFN gamma in the conjunctiva between the two strains . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Studies in IFN gammaR ( / ) mice indicated that IFN gamma was not required for the adjuvant effect of alpha GalCer . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
In this study , the roles of interferon ( IFN ) gamma and nitric oxide ( NO ) in the pathogenesis of SFV infection were assessed using mice deficient in inducible nitric oxide synthase ( iNOS / ) , an enzyme important in the production of NO , and mice deficient in IFN gamma receptor ( IFN gammaR / ) . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Interferon ( IFN ) gamma is a cytokine that plays a pivotal role in the defense against infection , and mutations in the gene coding for the ligand binding chain ( alpha , R 1 ) of the IFN gamma receptor ( IFNGR 1 ) confer suseptibility on infections caused by poorly virulent mycobacteria . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
T cell dependent protection against C . pneumoniae was weaker when IFN gammaR ( / ) CD 4 ( + ) or IFN gammaR ( / ) CD 8 ( + ) SC were inoculated into RAG 1 ( / ) / IFN gamma ( / ) mice . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
IFN gamma effects are regulated by IFN gamma receptor ( IFNGR ) expression . ^^^ IFN gamma is pro inflammatory at low IFNGR levels , but induces apoptosis in cells with high IFNGR levels . ^^^ Surface IFNGR alpha ( the binding chain ) and IFNGR beta ( signaling chain ) , as well as intracellular IFN gamma and IL 10 , were measured with flow cytometry . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
The role of IFN gamma receptor 1 ( IFNGR 1 ) and IFN gamma receptor 2 ( IFNGR 2 ) gene polymorphisms has not been studied in MS , and , for the IFNG gene polymorphism there is only one previous study , which incorporates clinical , but not imaging , data . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
In the present study we analyzed the expression of IFN gamma receptor ( IFN gammaR ) and signal transducer and activator of transcription 1 ( STAT 1 ) in 4 children with Mycobacterium tuberculosis infection of uncommon clinical presentation . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Migration of a rodent pathogenic T . brucei strain from the cerebral blood vessels into the brain parenchyma was impeded in IFN gamma ( / ) , IFN gamma receptor ( / ) ( IFN gammaR ( / ) ) , IL 12p40 ( / ) , and recombinant activating gene 1 ( / ) ( RAG 1 ( / ) ) mice as compared with their WT littermates despite higher levels of parasitemia in the mutant strains . ^^^ Parasites accumulated in the perivascular compartment , confined between the endothelial and the parenchymal basement membranes , in certain areas of the brains of IFN gamma ( / ) , IFN gammaR ( / ) , and RAG 1 ( / ) mice . ^^^ The number of CD4+ and CD8+ T cells infiltrating the brain parenchyma was also reduced in the IFN gamma ( / ) and IFN gammaR ( / ) mice . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
We have created a series of novel mutants of IFN gammaR 2 that have facilitated the identification of intracellular domains that control IFN gammaR 2 accumulation and IFN gamma responsiveness . ^^^ These IFN gammaR 2 mutants will allow fine dissection of the role of IFN gamma signaling in immunity . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Interestingly , Rac 1 appeared to associate with IFN gammaRalpha and augment the interaction of IFN gammaR with either STAT 1 or STAT 3 in response to IFN gamma . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Both IFN gamma and LIF are produced by maternal cells and tissues at the fetal maternal interface , whereas the IFN gamma receptors ( IFN gamma R 1 and IFN gamma R 2 ) and LIF receptor are abundantly expressed on the surface of placental trophoblasts . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
A nuclear localization sequence ( NLS ) in the type 2 interferon ( IFN ) IFN gamma , which is responsible for the nuclear translocation of both the ligand and the alpha subunit ( IFNGR 1 ) of the receptor complex , has previously been characterized and its role in signaling examined in detail . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Acute lethal colitis induced by Ad5IL12 was T cell mediated and IFN gamma receptor ( IFN gamma R ) dependent . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
In this study , we compared STAT 6 phosphorylation in wild type ( WT ) and IFN gamma receptor knockout ( IFNGR ( / ) ) Th 1 cells . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Previous research has shown that infection of mouse macrophages with Mycobacterium avium causes decreased expression of the IFN gamma receptor ( IFNGR ) . ^^^ In spite of the decrease in IFNGR 2 receptor expression , activation of Stat 1 activation by an optimal dose of IFN gamma was identical between control and TLR 2 stimulated RAW264 . 7 cells . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Mice with a deficiency in IFN gamma or IFN gamma receptor ( IFN gammaR ) are more susceptible to collagen induced arthritis ( CIA ) , an experimental autoimmune disease that relies on the use of complete Freund ' s adjuvant ( CFA ) . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
The pathogenetic importance of IFNs was determined by monitoring tumor development in mice genetically deficient in the IFN alpha / beta receptor ( IFN alpha / betaR KO ) or the IFN gamma receptor ( IFN gammaR KO ) . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Previous studies have indicated that Ctx induces intestinal interferon gamma ( IFN gamma ) production and that adjuvant properties require activation of the IFN gamma receptor ( IFNGR ) . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
These mimetics do not act through recognition by the extracellular domain of IFN gamma receptor but rather bind to the cytoplasmic domain of the receptor chain 1 , IFNGR 1 , and thereby initiate the cellular signaling . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Although it is well known that IFN gamma exerts its effects by binding to the IFN gamma receptor ( IFN gammaR ) , the role that IFN gammaR plays in the development of EAU has not been investigated . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
We show that development of IFN gamma producing B cells by either Th 1 cells or IL 12 / IL 18 is absolutely dependent on expression of the IFN gammaR and the T box transcription factor , T bet . ^^^ Interestingly , although T bet up regulation in developing B effector 1 ( Be 1 ) cells is controlled by IFN gammaR mediated signals , STAT 1 deficient B cells up regulate T bet and produce IFN gamma , indicating that additional transcriptional activators must be coupled to the IFN gammaR in B cells . ^^^ Finally , we show that although IL 12 / IL 18 or IFN gamma producing Th 1 cells are required to initiate transcription of the IFN gamma gene in B cells , sustained expression of IFN gamma and T bet by B cells is dependent on an IFN gamma / IFN gammaR / T bet autocrine feedback loop . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
This was the case , although peptide recall of primed IFN gamma ( / ) or IFN gammaR ( / ) CD 8 T cells up regulated pro TGF beta protein as measured by surface latency associated peptide expression but yet were unable to suppress . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
In addition , IFN gamma producing ( Th 1 ) Ag specific CD 4 T cells were generated in mice lacking the ligand binding chain of the IFN gammaR ( IFN gammaR 1 / ) or the signaling chain ( IFN gammaR 2 / ) . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Activation of STAT 1 and the IFN gamma response are thought to be mediated exclusively through the Y 440 motif of the human IFNGR 1 receptor subunit . ^^^ Contrary to this accepted dogma , here it is shown that IFNGR 1 with a mutant ( Y440F ) motif , when stably expressed in IFNGR 1 negative human fibroblasts at levels similar to wild type , can sustain a substantial IFN gamma response . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
We previously reported that treatment with a specific CXCR 4 antagonist , AMD 3100 , exerts a beneficial effect on the development of collagen induced arthritis ( CIA ) in the highly susceptible IFN gamma receptor deficient ( IFN gammaR KO ) mouse . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Proliferative enteropathy was reproduced in IFN gamma receptor knockout ( IFN gamma R ) mice by experimental infection with Lawsonia intracellularis ( L . intracellularis ) . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
This network encompasses 17 nodes , namely IFN gamma , IL 4 , IL 12 , IL 18 , IFN beta , IFN gammaR , IL 4R , IL 12R , IL 18R , IFN betaR , STAT 1 , STAT 6 , STAT 4 , IRAK , SOCS 1 , GATA 3 , and T bet , as well as their cross regulatory interactions . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Furthermore , increases in IFN gamma receptors ( IFNGR ) induced by IL 1beta were found to be dependent on NF kappaB transactivation . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
We analysed frequencies of two single nucleotide polymorphisms ( SNP ) in the interferon gamma ( IFN gamma ) receptor 1 ( IFNGR 1 ) gene promoter ( G 611A , T 56C ) in tuberculosis patients ( n = 244 ) and compared them with controls ( n = 521 ) . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
IFN gamma stimulation triggered a physical association between the IFN gammaR 1 and MyD 88 . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
First , interferon ( IFN ) gamma receptor ( IFN gamma R ) deficient mice were used to show that IFN gamma mediated immune activation is crucial for parasitemia control . ^^^ Downstream of IFN gamma R signaling , inducible NO synthase ( iNOS ) dependent trypanosome killing occurs , as is shown by the hypersusceptible phenotype of iNOS deficient mice . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Many of the biological activities of IFN gamma are mediated through the IFN gammaR 3 linked Jak Stat1alpha pathway . ^^^ To identify novel elements involved in IFN gamma cell regulation , the cytoplasmic domain of the R 2 subunit of the human IFN gammaR was used as bait in a yeast two hybrid screen of a human monocyte cDNA library . ^^^ Furthermore , immunoprecipitation using anti IFN gammaR 2 Abs showed that AxV interacted with IFN gammaR 2 to form a stable complex following incubation of cells with IFN gamma . ^^^ Taken together , these results suggest that through an inducible association with the R 2 subunit of the IFN gammaR , AxV modulates cellular responses to IFN gamma by modulating signaling through the Jak Stat 1 pathway . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Thus , expression of rejection induced transcripts is robust and consistent in allografts , reflecting the IFN gamma produced by the alloresponse locally and systemically , acting via host and donor IFN gammar , as well as local IFN gamma production induced by post operative stress . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
We investigated disease association with the genes encoding IFN gamma and its receptor subunits ( IFNG , IFNGR 1 , and IFNGR 2 ) . ^^^ The disease association with IFNGR 1 is novel , and together these findings support the hypothesis that genetically determined variation in both IFN gamma production and responsiveness influences the risk of developing tuberculosis . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
We found that STAT 1 / mice were even more susceptible to C . pneumoniae than IFN gamma / or IFN gammaR / mice . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
This nonredundant efficacy of CD 8 ( + ) T cell effector mechanisms in maintaining viral latency is explained as follows : ( 1 ) a subset of neurons that expresses IFN gamma receptors ( IFN gamma R ( + ) ) and Qa 1 responds to IFN gamma , but Qa 1 engagement of CD94 / NKG2a blocks LG exocytosis by CD 8 ( + ) T cells ; ( 2 ) another neuronal subset is responsive to LG because it lacks Qa 1 and is refractory to IFN gamma because it also lacks IFN gamma R . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
The IFN gamma Type 1 receptor ( IFNGR 1 ) was found to be associated with the TGF beta 1 Type 1 receptor ( TGF betaRI ) and was phosphorylated by the TGF betaRI . ^^^ Thus , multiple mechanisms are present for the TGF beta 1 mediated reduction of iNOS production , and we propose a novel mechanism for regulating inflammatory cytokine by an anti inflammatory cytokine , TGF beta 1 ; i . e . suppression of IFN gamma induced STAT 1 activation by an association of the IFNGR 1 with the TGF betaRI . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
The antineoplastic agent bryostatin 1 differentially regulates IFN gamma receptor subunits in monocytic cells : transcriptional and posttranscriptional control of IFN gamma R 2 . ^^^ Given the pivotal role that IFN gamma exerts on monocyte activation and in the initiation and outcome of the immune response , the induction of IFN gammaR 2 by Bryo 1 has significant implications in immunomodulation and could overcome some of the immune defects observed in cancer patients . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Secretion of IFN gamma by Th 1 and the expression of IFN gammaR on Tr 1 were critical for further Tr 1 differentiation , as demonstrated by mAb blocking and by analysis in IFN gamma ( / ) mice . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
The data revealed mixed T helper cell type 1 ( Th 1 ) and T helper cell type 2 ( Th 2 ) responses , as reflected by elevated expression of interferon ( IFN ) gamma , tumor necrosis factor ( TNF ) alpha , transforming growth factor ( TGF ) beta , interleukin ( IL ) 10 , IL 6 , and IL 4 mRNA , with minimal expression of IFN gamma receptor 1 ( IFN gamma R 1 ) mRNA in PKDL lesions , compared with that in normal skin tissue . ^^^ In the presence of elevated levels of IFN gamma and TNF alpha , interference with type 1 effector activity in PKDL may be due to minimal expression of the IFN gamma R 1 gene or the simultaneous presence of elevated levels of IL 10 , IL 6 , and TGF beta , which have counteracting effects . ^^^ After treatment , the restoration of IFN gamma R 1 at both mRNA and protein levels , coupled with down regulation of counteracting cytokines , may facilitate the action of signals associated with IFN gamma , yielding parasite clearance . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
IFN gamma and its receptor subunit IFNGR 1 are recruited to the IFN gamma activated sequence element at the promoter site of IFN gamma activated genes : evidence of transactivational activity in IFNGR 1 . ^^^ We have shown previously that IFN gamma and one of its receptor subunits , IFNGR 1 , are translocated to the nucleus , together with STAT1alpha as one macromolecular complex , via the classical importin dependent pathway . ^^^ In this study , we have identified the nuclear targets of IFN gamma and IFNGR 1 . ^^^ By chromatin immunoprecipitation followed by PCR , IFN gamma , its receptor subunit IFNGR 1 , and STAT1alpha were found to be associated with the IFN gamma activated sequence ( GAS ) in the promoter of two of the genes stimulated by IFN gamma . ^^^ Immunoprecipitated chromatin also showed the association of the IFN gamma , IFNGR 1 , and STAT1alpha on the same DNA sequence . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Identification of an interferon gamma receptor alpha chain sequence required for JAK 1 binding . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Assignment of interferon gamma receptor ( IFNGR 1 ) to human chromosome bands 6q24 . 1 > q24 . 2 by in situ hybridization . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Human interferon gamma receptor 1 ( IFNGR 1 ) deficiency is a newly identified autosomal recessive inherited immune disorder . ^^^ This review compares the infections found in IFNGR 1 deficient children to those in IFN gamma deficient or IFNGR 1 deficient mice . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
The role of interferon gamma in autoimmune diabetes was assessed by breeding a null mutation of the interferon gamma receptor alpha chain into the nonobese diabetic mouse strain , as well as into a simplified T cell receptor transgenic model of diabetes . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
TaqI RFLP in the interferon gamma receptor 1 gene ( IFNGR 1 ) on human chromosome 6q . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Human interferon gamma receptor 1 ( IFNGR 1 ) gene maps to chromosome region 6q23 6q24 . ^^^ The human interferon gamma receptor ( IFNGR 1 ) gene has been localized by in situ hybridization to chromosome 6 at q 23 q24 . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
OBJECTIVE : The guided selection strategy for isolation of human antibody ( Ab ) fragments specific for human interferon gamma receptor 1 ( IFNGR 1 ) from a cloned Ab VH and VL repertoire has been investigated . ^^^ RESULTS AND CONCLUSIONS : The VH region of murine monoclonal Ab ( IR gamma 1 ) against human IFNGR 1 was combined with human VL repertoire and used for selection of human VL regions . ^^^ A chimeric Fab consisting of kappa 2 and the murine IR gamma 1 VH region was highly IFNGR 1 specific and exerted the same epitope specificity and a comparable binding affinity as the parental murine Fab . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
The BCP cell lines display surface receptors for interferon gamma ( CD 119 ) , interleukin 7 ( CD 127 ) and FLT 3 ligand ( CD 135 ) . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
To examine the expression of the cytokine IL 4 and IL 5 receptors ( IL 4R and IL 5R ) in a nasal allergen challenge model and to contrast this with the expression of the receptor for the Th 1 type cytokine , interferon gamma receptor ( IFN gammaR ) , and 2 . to examine the effects of pretreatment with topical corticosteroid before allergen challenge on the expression of these same receptors . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
A complex formed between the extracellular human interferon gamma receptor alpha chain ( hIFNgammaR ) and the Fab fragment of the neutralizing antibody A 6 has been studied by site directed mutagenesis . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
To examine the expression of the cytokine IL 4 and IL 5 receptors ( IL 4R and IL 5R ) in a nasal allergen challenge model and to contrast this with the expression of the receptor for the Th 1 type cytokine , interferon gamma receptor ( IFN gammaR ) , and 2 . to examine the effects of pretreatment with topical corticosteroid before allergen challenge on the expression of these same receptors . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
BACKGROUND : We reported on the constitutive interferon gamma receptor ( IFN gammaR ) expression on eosinophils . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
The regulatory role of interferon gamma receptor ( IFN gammaR ) and tumor necrosis factor receptor ( TNFR ) mediated immune reactions for the activation of cerebral endothelial cells , microglia , and astrocytes was evaluated in a model of murine Toxoplasma encephalitis ( TE ) . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
In addition , we evaluated polymorphisms in 2 human genomic loci , which are associated with either severe or recurrent microbial infections ( interferon gamma receptor [ IFN gammaR ] and mannose binding lectin [ MBL ] ) ; these host genes were also amplified directly from the CSF specimens . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Linkage analysis on Con A blasts from ( BALB / c 10 CByB6F1 ) N 2 backcross progeny for ( 1 ) relative level of competitive inhibition of BALB / c NK lysis of syngeneic Con A blasts and ( 2 ) genotypes at polymorphic microsatellite markers distributed throughout the mouse genome mapped the Nktar gene locus to a 5 cM region of chromosome 10 containing the interferon gamma receptor ( Ifngr ) gene locus . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
In order to more fully understand the spectrum of responses expressed by macrophages when encountering these distinct stimuli , we investigated the similarities and differences between interferon gamma receptor ( IFN gammaR ) dependent macrophage activation and stimulation of macrophages through the Type A 1 scavenger receptor ( SR ) . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Radiation of the esophagus of C3H / HeNsd mice with 35 or 37 Gy of 6 MV 10 rays induces significantly increased RNA transcription for interleukin 1 ( Il 1 ) , tumor necrosis factor alpha ( Tnf ) , interferon gamma inducing factor ( Ifngr ) , and interferon gamma ( Ifng ) . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Analysis of antibody A 6 binding to the extracellular interferon gamma receptor alpha chain by alanine scanning mutagenesis and random mutagenesis with phage display . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Recently , recessive mutations in the interferon gamma receptor ligand binding chain ( IFNgR 1 ) , interferon gamma receptor signalling chain ( IFNgR 2 ) , interleukin 12 p 40 subunit ( IL 12p40 ) , and interleukin 12 receptor beta 1 chain ( IL 12Rb1 ) genes have been identified in a number of patients with disseminated BCG or NTM infection . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Different types of mutations in four genes ( IFNGR 1 , IFNGR 2 , IL12B , IL12RB1 ) have revealed both allelic and nonallelic heterogeneity and result in eight different disorders whose common pathogenic pathway is impaired interferon gamma ( IFNgamma ) mediated immunity . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
By Western blotting the plasma membrane raft fractions obtained using either non detergent or detergent based methods contained significant amounts of STAT 1 and STAT 3 ( up to approximately 10 % of the total cytoplasmic amount ) as well as the integral raft proteins caveolin 1 and flotillin 1 , the IL 6 receptor signal transducing chain gp 130 , the interferon gamma receptor alpha chain ( IFN gammaRalpha ) , and the chaperone glucose regulated protein 58 ( GRP58 / ER 60 / ERp57 ) . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Patients with a dominant small deletion ( 818del4 , hotspot ) in the interferon gamma receptor 1 ( IFNGR 1 ) gene ( 6q23 q 24 ) and increased susceptibility to mycobacterial infections have been recently reported . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Tumor development induced by MCA but not due to p 53 deficiency is increased in interferon gamma receptor ( IFN gammaR ) deficient mice . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
These lately discovered genes , relevant to immune disorders of mononuclear phagocytes and neutrophils , include defects in the interferon gamma ( IFNg ) / interleukin 12 ( IL 12 ) pathway , such as IFNg receptor ( IFNgR ) defects , IL 12 defect , IL 12 receptor ( IL 12R ) defect , and signal transducer and activator of transcription 1 ( STAT 1 ) defect . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Missense mutations of the interleukin 12 receptor beta 1 ( IL12RB1 ) and interferon gamma receptor 1 ( IFNGR 1 ) genes are not associated with susceptibility to lepromatous leprosy in Korea . ^^^ Interleukin 12 receptor beta 1 ( IL12RB1 ) , interleukin 12 receptor beta 2 ( IL12RB2 ) , and interferon gamma receptor 1 ( IFNGR 1 ) perform important roles in the host defense against intracellular pathogens such as Mycobacteria . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Apparently for the first time , the peripheral blood monocytes of individuals with active visceral leishmaniasis ( VL ) have been found to show reduced expression of interferon gamma receptor 1 ( IFNGR 1 ) . ^^^ Since interferon gamma is the main cytokine responsible for defence against leishmanial parasites , it was thought possible that effective antileishmanial drugs may up regulate IFNGR 1 . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
We identified a novel heterozygous single nucleotide substitution 1400 T right curved arrow C ( Leu 467 Pro ) in the seventh exon of the interferon gamma receptor 1 ( IFNGR 1 ) gene . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Direct fluorescence conjugated antibodies directed against intracellular cytokines ( interferon gamma , tumour necrosis factor [ TNF ] alpha , interleukin 1 ) and their receptors ( CD 119 , CD120a , CD121a ) were used . ^^^ For interferon gamma and CD 119 , no changes were seen . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
The interferon gamma ( IFNGR ) and interleukin 4 ( IL 4R ) cytokine receptors , in particular , direct the earliest stages of T helper commitment . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
A strong correlation exists between IFNGR 1 genotype , cellular responsiveness to interferon gamma , and clinical disease features . . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
These primary immunodeficiencies are caused by germline mutations in seven genes : ELA 2 , encoding a neutrophil elastase , and GFI 1 , encoding a regulator of ELA 2 ( mutations associated with severe congenital neutropenia ) ; CXCR 4 , encoding a chemokine receptor ( warts , hypogammaglobulinemia , infections and myelokathexis syndrome ) ; LCRR 8 , encoding a key protein for B cell development ( agammaglobulinemia ) ; IFNGR 1 , encoding the ligand binding chain of the interferon gamma receptor ; STAT 1 , encoding the signal transducer and activator of transcription 1 downstream from interferon gammaR 1 ( Mendelian susceptibility to mycobacterial diseases ) ; and IKBA , encoding IkappaBalpha , the inhibitor alpha of NF kappaB ( anhidrotic ectodermal dysplasia with immunodeficiency ) . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
IFNGR 1 , encoding the ligand binding chain of the receptor for interferon gamma , IFNgammaR 1 , is one such gene because interferon gamma is involved in the pathogenesis of the disease . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
We report evidence of a polymorphism in the promoter region of IFNGR 1 ( encoding interferon gamma receptor 1 ) that has opposite functional effects in different cellular contexts . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
A causative relationship between mutant IFNgR 1 alleles and impaired cellular response to IFNgamma in a compound heterozygous child . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Mouse strains with null mutations in the gamma interferon gene ( Ifng ) or the gamma interferon receptor gene ( Ifngr ) have been engineered . ^^^ However , direct comparisons between Ifng / ( GKO ) and Ifngr / ( RGKO ) mice have been problematic because previously available strains of these mice have had different genetic backgrounds ( i . e . , C57BL / 6 and BALB / c for GKO mice and 129 / Sv / / Ev for RGKO mice ) . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Several dysfunctional mutations have been identified in IFNgamma receptor genes ( IFNGR 1 and IFNGR 2 ) in relation to severe and selective infections with poorly pathogenic organisms . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
METHODS : We investigated the association of IFNG ( CA repeat polymorphism within the first intron ) , IRF 1 ( GT repeat polymorphism within the intron 7 ) , IFNGR 1 ( Val 14 Met ) , and IFNGR 2 ( Gln 64 Arg ) gene polymorphisms with atopic asthma in the Japanese child population . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
METHODS : The levels of interferon gamma receptor alpha ( IFNGR alpha ) on the peripheral lymphocyte membrane were assayed with flow cytometry . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
In the present study we investigated the possible presence of IFNgamma receptors ( IFNGR ) on mouse oocytes and preimplantation embryos . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Biological function was associated with activation of STAT1alpha and nuclear translocation of IFNgamma , IFNGR 1 and STAT1alpha . ^^^ Immunoprecipitation of cellular extracts with antibody to the nuclear transporter NPI 1 showed the formation of a complex with IFNgamma IFNGR 1 STAT1alpha . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
To identify a key genetic factor in the pathogenesis of sarcoidosis , we investigated single nucleotide polymorphisms within 10 candidate genes involved in type 1 immune process ( IFNA 17 , IFNB , IFNG , IFNGR 1 , IFNGR 2 , IL12B , IL12RB1 , IL12RB2 , ETA 1 , and NRAMP 1 ) in an association based study of 102 Japanese patients with sarcoidosis , 114 with tuberculosis , and 110 control subjects . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
The mimetic also shares with IFNgamma the ability to induce the activation and nuclear translocation of STAT1alpha and the IFNgamma receptor ( IFNGR ) 1 subunit . ^^^ We show that the mimetic mediates the nuclear transport of IFNGR 1 through its interaction with IFNGR 1 cytoplasmic region 253 287 via both the helical region and the NLS of IFNgamma ( 95 132 ) . ^^^ These data also show that the NLS of IFNgamma ( 95 132 ) can interact simultaneously with IFNGR 1 and the nuclear import machinery . ^^^ We found that in in vitro nuclear transport assays tyrosine phosphorylated STAT1alpha failed to undergo nuclear translocation in the presence of nuclear import factors , but was transported to nucleus in the presence of IFNgamma ( 95 132 ) and JAK 2 phosphorylated IFNGR 1 , to which STAT1alpha binds , as a complex of IFNgamma ( 95 132 ) / IFNGR 1 / STAT1alpha . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Tyrosine 419 of the IFNgamma receptor subunit 1 ( IFNGR 1 ) is required to activate both STATs , suggesting that STAT 1 and STAT 3 compete with each other for the same receptor phosphotyrosine motif . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
Upon stimulation , mouse IFNgamma receptor subunit 1 ( IFNGR 1 ) is phosphorylated on several cytoplasmic tyrosine residues , and Tyr ( 419 ) is required for signal transducer and activator of transcription ( STAT ) 1 activation in mouse embryo fibroblasts . ^^^ Several tyrosine to phenylalanine mutants of IFNGR 1 , expressed at normal levels in stable pools of IFNGR 1 null cells , were analyzed for the phosphorylation of STAT 1 during a 48 h period , and antiviral activity in response to IFNgamma was also measured . ^^^ Furthermore , constitutive overexpression of SOCS 1 inhibited IFNgamma dependent signaling only in cells expressing IFNGR 1 variants that included the Tyr ( 441 ) mutation . ^^^ Mutation of Tyr ( 441 ) also blocked the ability of SOCS 1 to bind to IFNGR 1 and JAK 2 in response to IFNgamma and the normal down regulation of STAT 1 activation and antiviral activity . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
DIM activated the expression of the IFNgamma receptor ( IFNGR 1 ) and IFNgamma responsive genes p 56 and p 69 oligoadenylate synthase ( OAS ) . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
IFNgamma , its receptor subunit IFNGR 1 , and transcription factor STAT1alpha are transported to the nucleus of cells as a complex where IFNgamma provides a classical polycationic nuclear localization sequence ( NLS ) for such transport . ^^^ The C terminus of IFNgamma , represented here by the mouse IFNgamma peptide , IFNgamma ( 95 132 ) , was capable of also forming a complex with IFNGR 1 and STAT1alpha when introduced intracellularly and provided the NLS signaling for nuclear transport . ^^^ Both IFNgamma and its peptide mimetics bind to an intracellular site , IFNGR 1 ( 253 287 ) , on the cytoplasmic domain of receptor subunit IFNGR 1 . ^^^ Finally , chromatin immunoprecipitations and reporter gene studies of IFNgamma and IFNgamma mimetic treated cells indicate that they , along with IFNGR 1 and STAT1alpha , bind to the GAS element of IFNgamma activated genes and participate in STAT1alpha transcription . ^^^
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
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Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P15260	Pubmed	SVM Score :0.0
NA