Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.53755918
We show that TNF alpha , while having no effect on its own , can synergize with IFN gamma to increase the level of promoter activity of a DRA CAT construct . 0.53755918^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.50970562
Induction of class 1 gene expression at the surface of tumor cells by interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) shows that TNF alpha can act in synergy with IFN gamma to induce DNA binding of both factors NF kappa B and KBFI to the class 1 gene enhancer and that this induction of transcriptional factors is correlated with enhancement of MHC class 1 mRNA transcription and cell surface antigen expression . . 0.50970562^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.69759888
Interferon gamma ( IFN gamma ) has been shown to exacerbate tumor necrosis factor alpha ( TNF alpha ) induced hepatotoxicity in vivo as well as act synergistically with TNF alpha in a variety of biological actions . 0.69759888^^^ In the present study we have examined interactions of IFN gamma with TNF alpha and the role of nitric oxide synthase ( NOS ) activity in the generation of an intracellular oxidant stress in isolated mouse hepatocytes . 0.52271544^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.53823423
An agonistic TNF R 1 specific antibody and recombinant human TNF alpha both exerted a cytotoxic effect when combined with IFN gamma , suggesting that the cytotoxicity was mediated through the TNF R 1 . 0.53823423^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.52122302
Synergy between IFN gamma and LPS was mainly dependent on tumour necrosis factor alpha ( TNF alpha ) secretion as the increase of the induction of the iNOS by IFN gamma plus LPS was associated with the increase of TNF alpha secretion and IFN gamma plus LPS induced TNF alpha secretion by microglial cells was decreased by the treatment with NaSal . 0.52122302^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.52141293
Furthermore , the linear association between IFN gamma and TNF alpha production was different between these two response groups ( rs = 0 . 19 for non responders and rs = 0 . 48 for responders ) . 0.52141293^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.71943912
It was found that interferon gamma ( IFN gamma ) , a cytokine produced by TH 1 lymphocytes , synergized with tumor necrosis factor alpha ( TNF alpha ) in RANTES expression and with IL 1 beta in MCP 1 synthesis . 0.71943912^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.56337072
In addition , when IFNgamma was combined with TNFalpha , IFNgamma inhibited the TNFalpha induced increase of ESM 1 mRNA level . 0.56337072^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Like ICAM 1 , VCAM 1 is also upregulated in vitro in renal cortical tubular and mesangial cells by TNF alpha , IL 1 and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunofluorescence staining of HEp 2 and HeLa cells grown in the presence of IFN alpha , IFN beta , or IFN gamma revealed an increase both in size and number of the Sp 100 protein containing nuclear dots , whereas no such effect was observed with cells treated with TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Agents , such as tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) or lipopolysaccharide ( LPS ) , which prime the macrophage for an enhanced zymosan triggered respiratory burst , increase tyrosine phosphorylation triggered by zymosan . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 3 induced a minor secretion of GM CSF whereas TNF , G CSF , M CSF and IFN gamma were unable to induce GM CSF secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines considered critical in pregnancy include interleukin 1 ( IL 1 ) , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma , granulocyte macrophage colony stimulating factor ( GM CSF ) , and colony stimulating factor ( CSF 1 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In order to evaluate the expression of cytokines in renal allograft biopsies , we performed immunocytochemical studies to detect activated cells positive for TNF alpha , IFN gamma , and IL 2R , using an alkaline phosphatase anti alkaline phosphatase technique ( APAAP ) . ^^^ There was a significant correlation between the number of positive cells reactive with monoclonal antibodies directed against TNF alpha , IFN gamma , and IL 2R and the presence of acute cellular rejection . ^^^ The mean number of infiltrating cells ( cells / mm2 ) positive for TNF alpha ( 9 . 2 + / 1 . 1 ) , IFN gamma ( 6 . 7 + / 1 . 7 ) , and IL 2R ( 31 . 2 + / 4 . 8 ) was significantly greater in acute cellular rejection episodes compared with nonrejecting kidneys ( 0 . 9 + / 0 . 2 , 1 . 2 + / 0 . 4 , and 8 . 8 + / 2 . 9 positive cells / mm2 for TNF alpha , IFN gamma , and IL 2R , respectively ) . ^^^ In two cases , in which acute cellular rejection was not sustained on clinical grounds but was diagnosed on histology , the expression of TNF alpha , IFN gamma , and IL 2R was similar to that observed in typical cellular rejection . ^^^ We conclude that TNF alpha , IFN gamma , and IL 2R are markedly expressed by activated mononuclear infiltrating cells in acute cellular rejection , and that these cytokines play an important role in allograft rejection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We also found that the effect of endogenous TNF alpha was enhanced by treatment of IFN gamma prior to the transfection with the TNF alpha gene . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Additionally , we report here that TNF alpha and interferon gamma ( IFN gamma ) synergise in the induction of both the lymphocyte adhesion receptor VCAM 1 , and the TNF alpha inducible neutrophil adhesion receptor intercellular adhesion molecule 1 , on all three endothelial cell types studied . ^^^ In contrast , we found that GM CSF secretion by endothelial cells treated with IFN gamma plus TNF alpha was markedly decreased when compared to the response induced by TNF alpha alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , this inflammation , which varies among mouse strains , is transient and it is not amplified or maintained by administration of cytokines , IFN gamma or TNF alpha , known to induce class 2 expression on thyrocytes . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have discovered that the seleno organic compounds induce interferon gamma ( IFN gamma ) , IFN alpha , tumor necrosis factor alpha ( TNF alpha ) and other cytokines in human peripheral blood leukocytes ( PBL ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , the role of tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) in the elimination of a primary infection with highly virulent Yersinia enterocolitica serotype 0 : 8 strain WA P has been investigated in C57BL / 6 mice . ^^^ The injection of anti TNF alpha or anti IFN gamma antibodies ( `` serotherapy ' ' ) prior to the intravenous challenge of a sublethal dose of Y . enterocolitica caused an increased bacterial net growth in the spleens , although this effect was more pronounced for anti TNF alpha treatment . ^^^ The later treatment with anti TNF alpha or anti IFN gamma antibodies on day 3 post infection likewise abrogated resistance to Y . enterocolitica and , subsequently , led to death from progressive infection . ^^^ Our data demonstrate for the first time that the endogenous production of both the cytokines TNF alpha and IFN gamma is required for the restriction of a primary Y . enterocolitica infection in mice . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The constitutively class 1 negative tumor cell line , Kgv , expresses H 2Dk in response to interferon gamma ( IFN gamma ) , but not in response to IFN alpha / beta , tumor necrosis factor , or lymphotoxin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neither IL 1 nor TNF alpha had significant effects on human chorionic gonadotrophin ( HCG ) stimulated progesterone production , whereas IFN gamma ( 1 10 ng / ml ) significantly reduced HCG stimulated progesterone production by 26 37 % . ^^^ In contrast , the combination of TNF alpha ( 1 ng / ml ) and IFN gamma ( 10 ng / ml ) acted synergistically to markedly inhibit HCG stimulated progesterone production by 81 % . ^^^ The combination of TNF alpha and IFN gamma also markedly inhibited follicle stimulating hormone ( FSH ) stimulated oestradiol production by 97 % , a significantly greater inhibition than that obtained with either cytokine alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The T lymphocyte derived cytokines TNF beta , IL 2 , IFN gamma and IL 4 were never detected in the cultures . ^^^ Further , a significant T lymphocyte production of TNF beta , TNF alpha , IFN gamma and IL 2 was found with peak production 12 48 hours after initiation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 3 , IL 5 , IL 6 , IL 8 , IL 10 , tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , inhibin and lipopolysaccharide ( LPS ) induced no thymocyte proliferation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Direct stimulation of cytokines ( IL 1 beta , TNF alpha , IL 6 , IL 2 , IFN gamma and GM CSF ) in whole blood : 2 . ^^^ The pattern of RA patient whole blood cells cultured in autologous blood is characterized by hyperactivity of the mononuclear cells with high secretion of IL 1 beta , TNF alpha and IL 6 and low production of IFN gamma , in comparison with the normal ( N ) and osteoarthrosis ( OA ) populations . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines ( IL 1 , sIL 2R , IL 3 , IL 6 , TNF alpha , IFN gamma , GM CSF and neopterin ) were measured in sera of 37 patients with hypertensive disorders of pregnancy , 10 healthy pregnant and 10 healthy non pregnant controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Confluent fourth passage mesothelial cell plates were exposed to varying concentrations of the recombinant human cytokines IL 1 alpha , TNF alpha , or IFN gamma , or Escherichia coli endotoxin ( LPS ) . ^^^ TNF alpha stimulated the release of the greatest quantity , whereas stimulation with IFN gamma and IL 1 alpha resulted in the release of lesser but still significant quantities of PMN chemotactic activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have assessed tumor necrosis factor alpha ( TNF alpha ) production and its autocrine effects on activation in two murine macrophage cell lines which have distinct responses to the activation stimuli interferon gamma ( IFN gamma ) and bacterial lipopolysaccharide ( LPS ) , and compared these responses to those observed in thioglycollate elicited peritoneal macrophages . ^^^ IFN gamma induced TNF alpha production in RAW 264 . 7 cells and this induction was regulated at the transcriptional level . ^^^ IFN gamma did not stimulate TNF alpha production in either WEHI 3 cells or peritoneal macrophages , although MHC class 2 antigen expression was induced . ^^^ We also assessed the ability of endogenous TNF alpha to serve as an autocrine regulator of two aspects of IFN gamma mediated macrophage activation , namely , induction of antibody independent tumoricidal activity and induction of MHC class 2 antigen expression . ^^^ These studies revealed that TNF alpha could act synergistically or antagonistically with IFN gamma in the regulation of these two functions , depending on both the macrophage population used and the function assessed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lymphokine analysis showed that supernatants of anti CD 3 mAb stimulated cultures had higher levels of TNF alpha and IFN gamma . ^^^ However , TNF alpha and IFN gamma alone or in combination could not mediate the inhibitory effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neutralization reactions with specific antibodies indicate the presence of tumor necrosis factor alpha ( TNF alpha ) in 14 15 ( 44 % inhibitable ) , interferon gamma ( IFN gamma ) and interleukin 2 ( IL 2 ) in 25 28 ( 65 % and 30 % neutralization , respectively ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Seven of them were found to be inducers of interferon gamma ( IFN gamma ) and / or tumor necrosis factor alpha ( TNF alpha ) in human peripheral blood leukocytes ( PBL ) cultures . ^^^ The simultaneous inductions of IFN gamma and TNF alpha were highly correlated , but independent on each other . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have recently discovered that these drugs are inducers of cytokines , mainly interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) and mitogenic interleukins in human peripheral blood leukocytes ( PBL ) but not in the mouse or rat lymphoid cells . ^^^ We described a production of IFN gamma and TNF alpha by various subsets of PBL stimulated with 2 phenyl 1 , 2 benzisoselenazol 3 ( 2H ) one ( ebselen ) or bis [ 2 ( N phenyl carbamoyl ) ] phenyl diselenide . ^^^ The relative concentrations of the subsets of lymphocytes or monocytes was important for the maximum production of both IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) , interferon alpha ( IFN alpha ) and interferon gamma ( IFN gamma ) receptors on human normal and scleroderma dermal fibroblasts in vitro . ^^^ Interferons alpha and gamma ( IFN alpha , IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) exert different regulatory effects on the proliferation and biosynthetic activities of human dermal fibroblasts . ^^^ Inasmuch as these cytokines bind to specific receptors in order to exert their activities , the expression of IFN alpha , IFN gamma and TNF alpha receptors on fibroblasts from human adult normal and scleroderma skin cultured in vitro were quantitated . ^^^ Tumor necrosis factor alpha ( TNF alpha ) , interferon alpha ( IFN alpha ) and interferon gamma ( IFN gamma ) receptors on human normal and scleroderma dermal fibroblasts in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human polymorphonuclear leukocytes ( PMN ) preincubated overnight with 100 U / mL gamma interferon ( IFN gamma ) had an increased metabolic response , as measured by iodination and / or superoxide production , to stimulation by tumor necrosis factor ( TNF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , formylmethionyl leucyl phenylalanine ( FMLP ) , opsonized zymosan , and lipopolysaccharide ( LPS ) , as compared with cells comparably preincubated in the absence of IFN gamma . ^^^ The decline in the staphylocidal activity of the stored PMN was also prevented in part by IFN gamma , as was the depressed adherence of PMN stimulated with phorbol myristate acetate ( PMA ) , FMLP , TNF , GM CSF , and LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Effects of Tumor Necrosis Factor ( TNF ) , Interleukin 1 ( IL 1 ) , Interleukin 6 ( IL 6 ) and Interferon gamma ( IFN gamma ) on the expression of Mn superoxide dismutase ( Mn SOD ) protein were investigated in human hepatoma cells , Hu H 1 , which revealed resistance to the cytotoxicity of TNF and IL 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokines , interferon gamma ( IFN gamma ) , interleukin 4 ( IL 4 ) , and tumor necrosis factor alpha ( TNF alpha ) , as well as cAMP elevating agents , 8 bromoadenosine 3 ' : 5 ' cyclic monophosphate ( 8BrcAMP ) , cholera toxin ( CT ) , and prostaglandin E 2 ( PGE 2 ) , suppressed the monocyte DNA synthesis due to CSF 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lymphocyte proliferation and TNF alpha production in response to the mitogen concanavalin A ( ConA ) were comparable in adults and infants , but ConA stimulated IFN gamma production in infants was diminished throughout the study period . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1 alpha , IL 1 beta , TNF alpha , and IL 2 co stimulated B cell proliferation and IgM , IgG , and IgA secretion , whereas IL 3 , IL 4 , IL 5 , IL 6 , IFN gamma , or GM CSF had weak or no activity in this regard . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It has been demonstrated that in human models , cytokines such as TNF , IL 1 , IFN gamma increase the adhesiveness of endothelium for cells of the immune and inflammatory system by stimulating the expression of cell adhesion molecules on endothelial cell surfaces . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PGE 1 , 2 , 3 and Misoprostol inhibited : ( 1 ) the mitogenic activity of interleukin 1 ( IL 1 ) for mouse thymocytes ; ( 2 ) spreading of mouse macrophages on glass ; ( 3 ) tumour necrosis factor ( TNF ) ( alpha and beta ) production by human peripheral blood mononuclear cells and rat macrophages ; ( 4 ) IL 1 production by rat and mouse peritoneal macrophages ; and ( 5 ) interferon gamma ( IFN gamma ) production by human peripheral blood mononuclear cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We measured the cytokines IL 1 alpha , IL 1 beta , IL 2 , IL 3 , IL 4 , IL 6 , IFN gamma , TNF alpha , granulocyte ( G ) / macrophage ( M ) CSF , G CSF , M CSF , and the lipid mediators leukotriene B 4 and platelet activating factor in the cellfree bronchoalveolar lavage fluid of mice during infection . ^^^ We found an early increase of IL 1 alpha , IL 1 beta , IL 6 , TNF alpha , GM CSF , IFN gamma , and leukotriene B 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To this end , fetal rat pancreatic islets containing a high fraction of beta cells were exposed in culture for 1 3 days to interleukin 1 beta ( IL 1 beta ) , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , interferon alpha ( IFN alpha ) , and interleukin 6 ( IL 6 ) at different concentrations . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The inhibition of cytopathological effects due to encephalomyocarditis virus ( EMCV ) by interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF ) in WISH cells using a microtiter assay was employed . ^^^ The model was used to analyze the characteristics of the dose effect relationships of IFN gamma , TNF , and their combination . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines including IL 2 , IL 4 , IL 6 , tumor necrosis factor alpha ( TNF alpha ) , GM CSF , and IFN gamma were each tested for capacity to modulate macrophage metalloproteinase and tissue inhibitor of metalloproteinases ( TIMP ) expression . ^^^ Despite the frequent antagonism observed between IL 4 and IFN gamma in other systems , the combination of these two agents lowered metalloproteinase biosynthesis dramatically , whereas IL 4 opposed the IFN gamma stimulated production of cytokines ( IL 1 and TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF and interferon gamma ( IFN gamma ) synergistically induced morphologic differentiation and monocytic antigen expression , while the antitype B receptor MoAb was synergistic for morphologic response , but not antigen expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present report , we examined the effect of interferon gamma ( IFN gamma ) on each type of TNF receptor . ^^^ Our results indicate that IFN gamma induces TNF receptors on both myeloid ( e . g . ^^^ Furthermore , by using antibodies specific to each type of receptor , we demonstrate that both TNF receptors are equally inducible by IFN alpha , IFN beta and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The production of IL 8 protein was detected by enzyme linked immunosorbent assay in the culture supernatants derived from eight of nine human gastric cancer cell lines stimulated with either interleukin 1 alpha ( IL 1 alpha ) , tumor necrosis factor alpha ( TNF alpha ) , or TNF alpha plus interferon gamma ( IFN gamma ) . ^^^ In some of the gastric cancer cell lines such as MKN 45 and KATO , TNF alpha plus IFN gamma synergistically induced the production of IL 8 . ^^^ In MKN 45 cells , synergistic increase of the steady state level of IL 8 mRNA by TNF alpha plus IFN gamma was not inhibited by cycloheximide treatment . ^^^ Scatchard analysis revealed that IFN gamma changed neither the number nor the affinity constant of TNF alpha binding sites on a gastric cancer cell line , suggesting that the synergism was a post receptor event . ^^^ Furthermore , synergistic induction of chloramphenicol acetyltransferase activity by TNF alpha plus IFN gamma was observed in MKN 45 that were transiently transfected with chimeric chloramphenicol acetyltransferase reporter genes driven by the transcriptional regulatory region of human IL 8 gene . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have found that anti TNF R 1 , but not anti TNF R 2 , Ab trigger antiviral activity in human hepatoma Hep G 2 cells and enhance the antiviral activity of IFN gamma in human lung fibroblast A 549 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Several cytokines , including TNF , IFN gamma , IL 2 and IL 4 , are inhibited by soluble receptors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The samples were tested for tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 alpha ( IL 1 alpha ) and 1 beta , IL 6 , IL 2 , interferon gamma ( IFN gamma ) , and the soluble receptors of IL 2 ( IL 2R ) and TNF ( TNF sR 55 and sR 75 ) , using various immunoassays and , occasionally , bioassays . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Activation of human macrophages for the killing of intracellular Trypanosoma cruzi by TNF alpha and IFN gamma through a nitric oxide dependent mechanism . ^^^ Pretreatment of human macrophages with recombinant IFN gamma and to a lesser extent with TNF alpha , induced a significant trypanocidal activity . ^^^ Furthermore , TNF alpha had a synergistic effect with IFN gamma on macrophage activation in T . cruzi killing . ^^^ Similarly , IFN gamma triggered the production of nitric oxide ( NO ) by macrophages , whereas TNF alpha was less effective , although it was also synergistic with IFN gamma . ^^^ Both NO production and trypanocidal activity , but not superoxide ( O 2 ) generation , induced in macrophages by TNF alpha or IFN gamma alone or in combination , were inhibited by N monomethyl L arginine ( N MMLA ) , a competitive inhibitor of NO synthase activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of four inflammatory cytokines , IL 1 , TNF , IFN gamma , and IL 6 , were assessed on the following functions of human vascular endothelial cells ( EC ) in culture : expression of procoagulant activity ( PCA ) , endothelial cell associated thrombomodulin ( TM ) , and IL 6 production . ^^^ IFN gamma , however , when added in combination with either IL 1 or TNF , modulated the effects of these cytokines ; INF gamma inhibited the PCA expression and enhanced the reduction of TM and the production of IL 6 , which were induced by either IL 1 or TNF . ^^^ These results suggest that both IL 1 and TNF are the major cytokines affecting the EC functions that determine the association between the coagulation and the inflammatory response , and that IFN gamma affects this phenomenon predominantly through the modification of the effects of these cytokines . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
THC at 5 or 10 micrograms / ml added to medium [ RPMI 1640 containing 10 ng LPS / ml , mouse IFN gamma ( 100 u / ml ) , and 0 . 5 % bovine serum albumin ( BSA ) ] used to induce TNF significantly decreased TNF alpha production by BALB / c mouse macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We transfected the human cell line 293 with the entire E 3 region of Ad 2 and investigated the influence of the cytokines TNF alpha and interferon gamma ( IFN gamma ) on cell surface expression of HLA class 1 and the EGF R . ^^^ Whereas IFN gamma treatment induced expression of HLA to some extent but not that of the EGF R , TNF alpha treatment augmented the reduction of these cell surface molecules . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neither tumor necrosis factor ( TNF ) , interleukin 1 ( IL 1 ) nor interferon gamma ( IFN gamma ) had such a stimulative effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It is shown here that the macrophage activating agents tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and lipopolysaccharide ( LPS ) can maximally inhibit colony stimulating factor 1 ( CSF 1 ) induced , murine bone marrow derived macrophage ( BMM ) DNA synthesis even when added 8 12 h after the growth factor , a period coinciding with the G1 / S phase border of the BMM cell cycle . ^^^ All three agents stimulated BMM protein synthesis ; TNF alpha and LPS , but not IFN gamma , stimulated BMM Na+ / H+ exchange and Na+ , K ( + ) ATPase activities , as well as c fos mRNA levels . ^^^ TNF alpha and LPS inhibited both CSF 1 stimulated urokinase type plasminogen activator ( u PA ) mRNA levels and u PA activity in BMM , whereas IFN gamma lowered only the u PA activity . ^^^ In contrast , LPS and IFN gamma , but not TNF alpha , inhibited CSF 1 induced BMM c myc mRNA levels , the lack of effect of TNF alpha dissociating the inhibition of DNA synthesis and decreased c myc mRNA expression for this cytokine . ^^^ These results indicate that certain biochemical responses are common to both growth factors and inhibitors of BMM DNA synthesis and that TNF alpha , IFN gamma , and LPS , even though they all have a common action in suppressing DNA synthesis , activate multiple signaling pathways in BMM , only some of which overlap or converge . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokines interleukin ( IL ) 1 beta IL 6 , tumor necrosis factor ( TNF ) alpha and interferon ( IFN ) gamma were identified in defined parts of LSG from patients but not in the corresponding parts of control glands : ( a ) LSG acinar epithelium expressed IL 1 beta , ( b ) blood vessels located in both normal LSG stroma and within lymphocytic infiltrates expressed IL 1 beta , IL 6 and IFN gamma , and ( c ) lymphocytic infiltrates expressed IL 1 beta , IL 6 and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
T cell clones derived from the CSF ( cerebrospinal fluid ) of MS ( multiple sclerosis ) patients have been analysed for the production of interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) , interleukin 2 ( IL 2 ) and interleukin 4 ( IL 4 ) . ^^^ Each CSF T clone ( both CD4+ and CD8+ ) produced substantial amounts of IFN gamma and especially TNF alpha compared with autologous peripheral T clones and liver infiltrating T clones from patients with chronic active hepatitis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Most TLC secreted substantial amounts of IFN gamma , IL 2 , TNF alpha , and GM CSF , but little or no IL 4 and IL 5 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pretreatment of the HEC layer with a variety of cytokines caused up regulation of lymphocyte adhesion with the effects ordered interferon gamma ( IFN gamma ) greater than tumor necrosis factor alpha ( TNF alpha ) greater than or equal to interleukin 1 beta ( IL 1 beta ) . ^^^ Study of the kinetics of lymphocyte adhesion showed that the effects of IFN gamma and TNF alpha were persistent and remained detectable 2 . 5 h after removal of the cytokines whereas the effects of IL 1 beta were transient and were not sustained beyond 1 h . ^^^ All of the cytokines used caused transient increases in the number of surface bound lymphocytes with IFN gamma greater than TNF alpha greater than or equal to IL 1 beta , however , the most dramatic effect was on the transmigration of lymphocytes across the HEC . ^^^ Both IFN gamma and TNF alpha caused sustained increased transmigration with IFN gamma having the greater effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) has been shown to augment the anti tumour effect of TNF . ^^^ I . ) injections of TNF plus IFN gamma in a murine tumour model and compared the toxicity and anti tumour effect with that seen with systemic administration . ^^^ Optimal mean survival and 30 day cure rate was seen with doses of 5 micrograms TNF alpha + 5000 U IFN gamma ( P < 0 . 05 vs . control or IFN gamma alone ) . ^^^ Tumour response after a single i . v . injection of 0 15 micrograms TNF + 5000 U IFN gamma was then compared with five daily p . ^^^ I . injections of the same dose of TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effect of IFN gamma and TNF alpha treatment of an ovarian carcinoma line on the sensitivity to lysis by specific CTL clones and non specific Tumor Associated Lymphocytes ( TAL ) , isolated from the ascites fluid , was analyzed . ^^^ Pretreatment with IFN gamma alone , or in combination with TNF alpha , rendered the carcinoma line less susceptible to lysis by the autologous TAL line . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of ICAM 1 that is found on many different cells such as melanocytes and melanoma cells is induced by various cytokines , including interferon gamma ( IFN gamma ) , interleukin ( IL ) 1 and tumor necrosis factor alpha ( TNF alpha ) . ^^^ IFN gamma , IL 1 , IL 7 , TNF alpha , and TNF beta significantly upregulated ICAM 1 expression in a dose dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This is surprising since in vitro the expression of these three molecules is equally induced by IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Several cytokines , such as IL 1 , TNF alpha or IFN gamma , have been shown to increase the ICAM 1 expression on cultured endothelial cells . ^^^ Both IFN gamma and TNF alpha are able to decrease the proliferation of the endothelial cell line , but IL 4 could not modify this response when added together with the other cytokines . ^^^ Taken together we show in this paper that IL 4 decreases IFN gamma but not TNF alpha induced up regulation of ICAM 1 expression on EA . hy 926 cells . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although the expression of IL 2 , IL 3 , IFN gamma , granulocyte macrophage colony stimulating factor and tumor necrosis factor alpha could be detected in CD4+ and CD8+ clones at the mRNA level following reverse transcription and polymerase chain reaction amplification , neither the secreted nor mRNA expression of IL 4 or IL 6 was detected in any of the tested clones . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1 , IL 6 , tumour necrosis factor alpha ( TNF alpha ) and IFN alpha , used alone or in combination , did not induce ICAM 1 expression , neither did they inhibit the IFN gamma induced expression of this adhesion molecule on HepG 2 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The ICAM 1 molecule expression was increased after the activation of the cells with recombinant murine gamma interferon ( IFN gamma ) , tumor necrosis factor ( TNF alpha ) , and interleukin 1 alpha ( IL 1 alpha ) in a dose dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , ICAM 1 expression on two of the three cell lines ( KG 1 and HL 60 ) was inducible by TNF alpha to a much higher degree than by IFN gamma , whereas the results with U 937 were comparable with those obtained for normal monocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These CD8+ T cells released IFN gamma in culture without antigen stimulation and were thought to induce tumour necrosis factor alpha ( TNF alpha ) production by the cells in the liver . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Ultraviolet radiation effects on human keratinocyte ICAM 1 expression : UV induced inhibition of cytokine induced ICAM 1 mRNA expression is transient , differentially restored for IFN gamma versus TNF alpha , and followed by ICAM 1 induction via a TNF alpha like pathway . ^^^ Accordingly , KC ICAM 1 expression is known to be induced in vivo and in vitro by cytokines either via the TNF alpha / TNF beta or via the IFN gamma mediated pathway . ^^^ Accordingly , UV light ( 0 100 J / m2 ) inhibited IFN gamma as well as TNF alpha induced ICAM 1 mRNA expression , if KC were cytokine stimulated immediately after irradiation . ^^^ After a 12 h incubation period , however , IFN gamma responsiveness was found to be restored in irradiated cells , whereas restoration of responsiveness to TNF alpha required at least a 24 h recovery phase . ^^^ In addition , this increase could be superinduced by stimulation of irradiated KC with IFN gamma , but not with TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inhibitors of BMM proliferation ( LPS , TNF alpha , IFN gamma , and cAMP elevating agents ) suppressed CSF 1 induced expression of M 1 , M 2 , and PCNA mRNA measured at 22 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Purified naive and memory CD 4 T cells from healthy donors , HIV+ asymptomatic carriers and AIDS patients were examined for their proliferative activity and their pattern of cytokine secretion ( IL 4 , IL 6 , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) ) upon stimulation with phytohaemagglutinin ( PHA ) , phorbol myristate acetate ( PMA ) and cross linked anti CD 3 MoAb , in the presence of recombinant IL 2 ( rIL 2 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha alone , and in synergistic combination with IFN gamma , also upregulated the expression of HaCaT ICAM 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Shedding of ICAM 1 occurred after stimulation of melanoma cells with TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of intercellular adhesion molecule 1 ( ICAM 1 ) by human cerebral endothelium was studied in primary cultures of human brain microvessel endothelial cells following treatment with bacterial lipopolysaccharide ( LPS ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 beta ( IL 1 beta ) and interferon gamma ( IFN gamma ) . ^^^ Stimulation with LPS and cytokines induces upregulation of ICAM 1 which is minimal with IFN gamma and maximal with LPS or a combination of IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma alone or in combination with TNF alpha induced the expression of HLA DR and ICAM 1 reaching a plateau after 48 h , followed by HLA DP and even later HLA DQ . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 4 , IL 6 , GM CSF and IFN gamma had no effect on TNF alpha production by T cells activated via either pathway . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
As previously shown for NK cells , activated B cells induce IFN gamma and TNF alpha production in non MHC restricted cytotoxic T lymphocytes ( NrCTL ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , synergy between these eosinophil survival factors and TNF ( alpha and beta ) was found mainly responsible for ICAM 1 induction , whereas synergy between IL 3 and IFN gamma occurred for HLA DR induction . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Preincubation of tumor cells with IFN gamma , IFN alpha or TNF alpha significantly decreased the susceptibility of each tumor cell line to lysis by LAK cells , and the change in sensitivity did not correlate with the expression of HLA antigens or intercellular adhesion molecule 1 ( ICAM 1 ) on the surface of tumor cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using flow cytometric analysis , human astrocyte cultures were examined for basal and cytokine [ interferon gamma ( IFN gamma ) , interleukin 1 beta ( IL 1 beta ) , and tumor necrosis factor alpha ( TNF alpha ) ] induced expression of molecules that may be involved in astrocyte T lymphocyte interactions . ^^^ Cultured human astrocytes spontaneously expressed major histocompatibility complex ( MHC ) class 1 antigens and variable levels of MHC class 2 ; MHC class 1 levels were increased upon IFN gamma and TNF alpha treatment , whereas MHC class 2 antigens were induced on most of the astrocytes by IFN gamma . ^^^ Among the molecules involved in antigen independent interactions between T lymphocytes and target cells , lymphocyte function associated molecule 3 ( LFA 3 ) was spontaneously expressed by most cultured human astrocytes , whereas intercellular adhesion molecule 1 ( ICAM 1 ) was present at variable levels in non stimulated astrocytes and was greatly induced by IFN gamma , TNF alpha , and IL 1 beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Validation experiments with human umbilical vein endothelial cells ( HUVECs ) demonstrated little detectable ICAM 1 expression on unstimulated cells or on cells incubated with interferon gamma ( IFN gamma ) , but HUVEC monolayers responded to interleukin 1 beta ( IL 1 beta ) or tumor necrosis factor alpha ( TNF alpha ) with significant increases in ICAM 1 and ICAM 1 dependent adherence of polymorphonuclear leukocytes ( PMNs ) . ^^^ HTEC monolayers also exhibited no significant basal ICAM 1 expression but , in contrast to HUVEC monolayers , had marked increases in ICAM 1 expression and ICAM 1 dependent PMN adherence only after incubation with IFN gamma ( and not after IL 1 beta or TNF alpha ) treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Because impaired ability of these cells to produce cytokines could be responsible for their functional incompetence , spontaneous and in vitro induced production of interleukin 2 ( IL 2 ) , interleukin 1 beta ( IL 1 beta ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) by TIL , LNL from tumor free as well as tumor involved lymph nodes ( LN ) , and peripheral blood lymphocytes ( PBL ) were measured . ^^^ In situ hybridization for cytokine mRNA performed with tumor tissues , and LN of patients with HNC showed that TIL as well as LNL localized in the immediate proximity of the tumor were activated , as evidenced by the expression of mRNA for IL 2 , IFN gamma , IL 1 beta , TNF alpha , and both alpha and beta chains of the IL 2 receptor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Flow cytometric analysis demonstrated constitutional expression of class 1 antigen on both cell lines , which was enhanced by interferon alpha ( IFN alpha ) , IFN gamma and tumor necrosis factor alpha ( TNF alpha ) . ^^^ A 4 h 51Cr release cytotoxicity assay demonstrated that pretreatment of both cell lines with IFN gamma or IFN alpha , but not with TNF alpha , decreased their susceptibility to LAK cells . ^^^ IFN gamma treatment decreased the susceptibility of ACHN cells in a dose dependent manner . `` Cold ' ' target competition assay clearly showed that IFN gamma but not TNF alpha pretreated cells compete less effectively than do untreated target cells . ^^^ Pretreatment with IFN gamma , however , increased expression of intercellular adhesion molecule 1 ( ICAM 1 ) to a degree comparable to that with TNF alpha . ^^^ Northern blot analyses using a 520 base pair ICAM 1 cDNA as a probe demonstrated that more 3 . 3 kb mRNA is expressed in IFN gamma and TNF alpha pretreated cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation of IFN gamma , TNF alpha , and TNF beta secretion in IL 2 activated T cells : costimulatory roles for LFA 1 , LFA 2 , CD 44 , and CD 45 molecules . ^^^ In addition , we demonstrate that at least four other structurally unrelated molecules , in addition to the TCR CD 3 complex , on LAK T cells participate in the stimulation of IFN gamma , TNF alpha , and TNF beta production . ^^^ Our data suggest that these three molecules enhance IFN gamma , TNF alpha , and TNF beta production by augmenting LAK T cell to tumor cell adhesion and also by functioning as costimulators . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pretreatment of HUVEC with interferon gamma ( IFN gamma ) , but not with IL 1 or tumour necrosis factor alpha ( TNF alpha ) , increased their ability to bind IgG of sera from patients with NE and acute febrile illnesses . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
FcR expression as mRNA and surface protein was therefore examined in the myelomonocytic cell line , U 937 , after stimulation with phorbol ester ( PMA ) , in the presence of seven different cytokines ( interferon gamma [ IFN gamma ] , IFN alpha , granulocyte macrophage colony stimulating factor [ GM CSF ] , tumour necrosis factor alpha [ TNF alpha ] , TNF beta , interleukin beta [ IL 1 beta ] , IL 2 ) or dexamethasone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
All clones transcribe mRNA for cytokines capable of inducing class 2 and ICAM 1 ( IL 4 , TNF alpha , IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of alpha 6 beta 1 integrin , the laminin receptor , on subsets of normal murine lung fibroblasts and its upregulation by the inflammatory cytokines IFN gamma and TNF alpha . ^^^ We now report the following findings : 1 ) the laminin receptor , alpha 6 beta 1 , is present on 20 30 % of freshly isolated normal murine lung cells in all three murine strains tested ; 2 ) established Thy 1+ and Thy 1 murine lung fibroblast subsets and clones constitutively express alpha 6 beta 1 at varied levels ; and 3 ) alpha 6 beta 1 expression on fibroblast lines and clones can be upregulated by treatment with IFN gamma or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Limiting dilution analysis was used to estimate the frequency of clonogenic Ag specific CD4+ T lymphocytes in draining lymph nodes of mice over the course of infection with Leishmania major , and to measure the production of IL 2 , IL 3 , IL 4 , IFN gamma , and TNF by the resultant clones . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have recently shown that this increased TNF release and macrophage accumulation are schematically made of two components , each mediated by different cytokines presumably released by stimulated CD4+ T lymphocytes : ( a ) a quantitative component : increased accumulation of macrophages results from the concomitant release of IL 3 and GM CSF , and ( b ) a qualitative component : macrophage number has not only to be raised , but macrophages need to be activated by IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Forty eight hours after activation , costimulated CD4+ cells demonstrated increased numbers of cells positive for surface IL 2R alpha chain , IL 2R beta chain , IFN gamma receptors , and TNF alpha receptors . ^^^ Anti CD 3 plus anti CD11a stimulation consistently induced the highest secretion of IL 2 and IFN gamma , whereas variation in secretion of TNF alpha and IL 4 between different donors was noted . ^^^ Amounts of IL 2 , IL 4 , and TNF alpha , but not IFN gamma , secreted also correlated with the levels of mRNA measured in the cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TA 3 inhibited spleen T cell adhesion to unstimulated EC by 30 % and to IFN gamma , TNF alpha , IL 1 alpha , and LPS stimulated EC by 50 to 60 % but inhibited sPEL EC adhesion by only 10 % . ^^^ The migration of spleen T cells to delayed type hypersensitivity and skin sites injected with LPS , poly 1 : C , IFN gamma , IFN alpha / beta , and TNF was inhibited by 72 to 88 % by TA 3 , and was decreased by 50 % to peripheral LN . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Role of T cells , TNF alpha and IFN gamma in recall of immunity to oral challenge with virulent salmonellae in mice vaccinated with live attenuated aro Salmonella vaccines . ^^^ Administration of anti IFN gamma or anti TNF alpha antibodies also impaired recall of immunity , exacerbating a secondary infection in vaccinated mice . ^^^ Surprisingly , IFN gamma was detectable in sera of both controls and T cell depleted mice on day 8 of the secondary infection , as well as in sera of anti TNF alpha treated mice on day 6 of infection . ^^^ The results indicate that T cells , IFN gamma and TNF alpha are all important in the specific recall of immunity to virulent salmonellae conferred by immunization with live vaccines , with the effect of T cell and IFN gamma depletion ( marked macrophage infiltration ) being qualitatively very different from that of TNF alpha neutralization ( no mononuclear infiltrate or granuloma formation ) . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There is no response to TNF , IL 1 and a number of other cytokines and growth factors , and only a negligible response to IFN gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , indomethacin , a well known inhibitor of prostaglandin synthesis , prevented the increase in lipolysis induced by TNF , IL 1 , IFN alpha , IFN beta , or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1 , TNF , IFN gamma , granulocyte macrophage CSF and , to a lesser extent , macrophage CSF , as well as inactivated streptococci , also induced MCP gene expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human NB cells may differentiate in vitro under treatment with biological agents , as gamma interferon ( IFN gamma ) and tumor necrosis factor ( TNF ) . ^^^ Here we demonstrate that a combination of IFN gamma plus TNF causes extensive and generalized differentiation of NB cells toward a neuronal phenotype . ^^^ Both IFN gamma and TNF , given alone , moderately reduced cell growth and induced partial morphological maturation . ^^^ Treatment of NB cell lines with IFN gamma / TNF results in the induction of a differentiated phenotype , as indicated by the increased expression of the Mr 160 , 000 and 200 , 000 neurofilament proteins and that of microtubule associated proteins . ^^^ These results suggest that the combination of IFN gamma and TNF should be considered for experimental clinical trials in neuroblastoma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Those T cell populations exhibiting sustained growth were then evaluated for cell surface phenotype by FACS ; for allospecific cytotoxicity by 51Cr release ; for a proliferative response to alloantigen by incorporation of [ 3H ] thymidine ; and for secretion of IL 2 , IL 4 , IFN gamma , and TNF alpha in response to alloantigenic stimulation by ELISA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When the cells were stimulated by phorbol ester ( phorbol 12 myristate 13 acetate , PMA ) plus calcium ionophore ( ionomycin ) , FK 506 and CsA inhibited , in a dose dependent manner , the production of IL 2 , IL 4 , IL 5 , IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore we demonstrate that ( a ) CD 56 molecule is involved in the adhesion of CD 3 CD2 CD11a / CD18 thymic cells , but not of peripheral CD 3 CD56+ lymphocytes , to untreated or IFN gamma and / or TNF alpha treated endothelium , ( b ) anti CDw49d mAb could inhibit the adhesion of this thymus derived population to either IFN gamma or TNF alpha treated endothelial cells but not to untreated endothelium , and ( c ) CD 56 antigen expressed by these cultured thymocytes has a sialic acid content different from that of peripheral lymphocytes . ^^^ Finally , CD 3 CD2 CD11a / CD18 thymocytes mobilized Ca2+ and released TNF alpha and IFN gamma after treatment with lectins . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro , the expression of both molecules was enhanced by interleukin 1 ( IL 1 ) , tumour necrosis factor ( TNF ) and interferon gamma ( IFN gamma ) and cytokine treated thyroid cells were more resistant to lysis by homologous complement . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma , IL 1 beta and TNF alpha produced a clear enhancement of ICAM 1 expression . ^^^ When tested in combination , IL 1 beta and TNF alpha were additive to the IFN gamma effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
None of the other recombinant lymphokines tested ( IL 1 , IL 2 , IL 3 , IL 6 , IFN alpha , and IFN gamma , granulocyte / macrophage CSF , transforming growth factor beta , TNF alpha , and lymphotoxin ) decreased CD 20 expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The release of sCD 14 from cultured monocytes was measured using LPS , TNF alpha ( tumour necrosis factor ) , IL 1 , 4 and 6 ( interleukin 1 , 4 and 6 ) and IFN gamma ( interferon gamma ) as stimulators . ^^^ Addition of LPS and TNF alpha led to a dose dependent increase in sCD 14 levels in the culture supernatant , whereas IL 1 , IL 6 and IFN gamma had no significant effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Induction of germline epsilon transcription in highly purified B cells seems to be a specific property of IL 4 , since none of the other cytokines tested [ IL 1 alpha , beta , IL 2 , IL 3 , IL 5 , IL 6 , IL 7 , IL 9 , IL 10 , G CSF , GM CSF , M CSF , IFN gamma , IFN alpha , tumor necrosis factor ( TNF ) alpha , and transforming growth factor ( TGF ) beta ] were effective . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Thus , IFN gamma appears to stimulate the early stage of myelopoiesis by enhancing the frequency of growth factor responding cells but , unlike tumor necrosis factor alpha ( TNF alpha ) , does not alter cell differentiation . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cultured FDC spontaneously produced low levels of IL 6 , but did not produce IL 1 alpha or TNF alpha ; however , after treatment with either IFN gamma or LPS , they produced more IL 6 . ^^^ The expression of CD 54 ( ICAM 1 ) was elevated by treating the cultured FDC with either TNF alpha , IL 1 beta , IFN gamma or granulocyte macrophage CSF ; in contrast , IL 4 had no effect on CD 54 but rather up regulated expression of VCAM 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cross linking of MHC class 2 molecules synergized with submitogenic amounts of anti CD 3 mAb in causing proliferation and secretion of the cytokines IL 2 , IL 3 , IFN gamma , and TNF alpha by MHC class 2 alloreactive T cell lines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We show here the induction of NO biosynthesis in freshly isolated human hepatocytes ( HC ) after stimulation with interleukin 1 , tumor necrosis factor ( TNF ) , IFN gamma , and endotoxin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Whereas other IFN ( alpha and beta ) reproduced the inhibitory effect of IFN gamma , IL 1 and TNF had no effect on hCG stimulated progesterone secretion at concentrations of 1 and 10 ng / mL . ^^^ Unlike their effects on hCG stimulated progesterone secretion , IL 1 and TNF reproduced the inhibitory effect of IFN gamma on FSH stimulated estradiol secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1 alpha , TNF alpha , IFN gamma , but not IL 4 , significantly up regulated the membrane expression of CD 40 on cultured TEC . ^^^ The regulation of CD 40 was similar to that of ICAM 1 , and contrasted with that of MHC class 2 Ag , which could only be induced by IFN gamma but not by IL 1 , TNF , or IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The NK cells were also found to express IFN gamma , transforming growth factor beta 1 ( TGF beta 1 ) , and tumor necrosis factor alpha ( TNF alpha ) mRNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Preliminary studies on the interaction of TNF alpha and IFN gamma with alpha 2 macroglobulin . ^^^ The binding of 125I labelled recombinant human TNF alpha and IFN gamma to isolated human blood alpha 2 macroglobulin has been investigated using molecular sieving procedures and non denaturing PA gel electrophoresis in combination with autoradiography . ^^^ PAGE / SDS gel investigations indicated that TNF alpha bound non covalently while the IFN gamma interaction was covalent in nature . ^^^ Preliminary competition studies also indicate that cold TNF alpha and IL 2 are more effective than cold IFN gamma at inhibiting the binding of labelled IFN gamma to alpha 2M . ^^^ Bioassays revealed that `` native ' ' alpha 2M or its derivatives at 2 mg / ml concentration did not impair the antiproliferative effects of TNF alpha and IFN gamma on susceptible bladder tumour cell lines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Additionally , a number of lymphokines were detected that directly influence epithelial function ( IFN gamma , TNF alpha , and TGF beta 1 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
VCAM 1 expression was further increased by exposure to IL 1 beta , TNF alpha , IL 4 , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The antifungal azole drugs itraconazole ( itra ; R 51 , 211 ) , fluconazole ( flu ; UK 49 , 858 ) , ketoconazole ( keto ) and micronazole ( mico ) have been investigated for their suppressive influence on the gene expression of the immunoregulatory cytokines IL 2 , IL 4 , IL 9 , GM CSF , TNF alpha , IFN gamma , as well as both chains of the IL 2 receptor in human PBMC and of the cytokines in the human keratinocyte cell line HaCat 17 . 5 . ^^^ While 1 microgram / ml CSA and 0 . 1 microgram / ml FK 506 completely suppressed PHA stimulated accumulation of mRNA for IL 2 , IL 4 , IL 9 , GM CSF , TNF alpha and IFN gamma in PBMC , flu , keto and TGF beta failed to inhibit any ( except TNF alpha blocked by TGF beta ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The stability and recovery of six human recombinant cytokines ( tumour necrosis factor ( TNF ) , interferon alpha ( IFN alpha ) , IFN gamma , interleukin 1 alpha ( IL 1 alpha ) , IL 1 beta , and IL 6 ) from whole blood was investigated with a view to optimizing blood collection and storage procedures prior to performing immunoassays . ^^^ The stability and recovery of the spiked cytokines varied from least stable to most stable spiked cytokine as follows ; TNF alpha less than IL 6 less than IFN gamma less than IL 1 alpha less than IFN alpha less than IL 1 beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In murine macrophages IFN gamma synergizes with TNF alpha or lipopolysaccharide to induce robust increases in calcium independent NO synthase activity . ^^^ In contrast to murine macrophages , IFN gamma inhibited TNF alpha stimulated induction of endothelial NO synthase activity in a concentration dependent manner . ^^^ However , cotreatment with IFN gamma potentiated the stimulatory effect of TNF alpha on BAEC ET 1 mRNA transcript levels and ET release . ^^^ In contrast , pretreatment of cells with IFN gamma for 16 24 h blunted the stimulatory effect of TNF alpha . ^^^ These findings suggest that endothelial cell expression of vasoactive mediators is modified by the temporal interplay of at least two immune mediators , IFN gamma and TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PMN incubated with interleukin 1 beta ( IL 1 beta ) , tumor necrosis factor , granulocyte macrophage colony stimulating factor ( CSF ) , granulocyte CSF , and interferon gamma ( IFN gamma ) , but not with prototypic chemoattractants ( fMLP , recombinant C5a , and IL 8 ) , showed a marked increase in survival , with values ranging at 72 hours of incubation from 89 . 5 % + / 5 . 8 % for IL 1 beta to 47 . 6 % + / 6 . 4 % for IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effect of the cytokines interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , granulocyte monocyte colony stimulating factor ( GM CSF ) and interleukin 1 ( IL 1 ) on the expression of CD 14 or HLA DR was different : IFN gamma strongly upregulated HLA DR expression and down regulated CD 14 expression while TNF alpha , GM CSF and IL 1 mainly stimulated CD 14 expression on bone marrow mononuclear cells . ^^^ Our data suggest that RA bone marrow mononuclear cells exhibit an activated phenotype and that TNF alpha GM CSF and IL 1 mainly stimulate the differentiation of bone marrow macrophages whereas IFN gamma activates them . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of LPS and mAb 5D3 as a trigger signal for IFN gamma primed macrophages were associated with production of TNF activity in culture fluids and inhibited by mAb against rTNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Autologous mixed lymphocyte tumor cell cultures ( MLTC ) were initiated with cytokine ( IFN gamma and TNF alpha ) treated ex vivo tumor cells of lung , ovarian , breast and stomach carcinomas . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
As the level of expression of these molecules can be altered during inflammation , a series of experiments was performed to examine the effects of the inflammatory cytokines interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) on adhesion molecules expressed by cultured human renal tubular epithelial cells . ^^^ Incubation with TNF alpha increased expression of both ICAM 1 and LFA 3 ; IFN gamma synergized with TNF alpha to further augment expression of these molecules . ^^^ Peripheral blood lymphocytes ( PBL ) showed an enhanced binding to allogeneic renal epithelial cell monolayers which had been pretreated with IFN gamma or TNF alpha . ^^^ MoAbs specific for ICAM 1 or its ligand LFA 1 inhibited adhesion of PBL to either IFN gamma or TNF alpha pretreated renal cells . ^^^ These results suggest that the inflammatory cytokines IFN gamma and TNF alpha up regulate expression of functional ICAM 1 and LFA 3 molecules which can augment the binding of potentially graft damaging lymphoid cells to renal tubular epithelial cells . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of mice with pure TNF alpha ( 0 . 2 microgram ) , IFN gamma ( 2 , 000 U ) , or a combination of the two did not induce the hepatic NOS , but treatment with the combination led to significant mortality ( probability of survival at 22 h , 0 . 32 ) . ^^^ Thus , the level of induction of NOS within the liver either in sepsis or by the coadministration of TNF alpha and IFN gamma does not correlate with death . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The criteria used to identify primed or memory T cells included activation dependent increases in the expression of CD 44 , LFA 1 , and the early activation Ag , CD 69 , and decreases in the expression of Mel 14 and CD45RB , as well as quantitative differences in the in vitro production of IFN gamma and the TNF alpha by stimulated cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cultured human mesothelial cells express appreciable levels of intercellular adhesion molecule 1 ( ICAM 1 ) and vascular cell adhesion molecule 1 ( VCAM 1 ) , and these were increased by in vitro exposure to tumor necrosis factor ( TNF ) , interferon gamma ( IFN gamma ) , or TNF and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Synthesis and expression of HLA class 1 molecules is increased in human endothelial cells by IFN beta , IFN gamma , and TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Gold salt treatment led to a slightly delayed and enhanced secretion of TNF alpha and IL 1 beta , an enhanced secretion of IL 2 and a restored secretion of IFN gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Whereas the suppressive effect on 83 / 1 cells is due to IFN gamma alone the suppression of BSA specific lymph node cells can be simulated neither by IFN gamma nor the combination of IFN gamma and TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study follows our previous investigation describing the production of four cytokines ( IL 2 , IL 4 , IFN gamma , and TNF alpha ) by subsets of thymocytes defined by the expression of CD 3 , 4 , 8 , and 25 . ^^^ The CD44+ population includes very immature precursor T cells and produced high titers of IL 2 , TNF alpha , and IFN gamma upon activation with calcium ionophore and phorbol ester . ^^^ These cells produced cytokines upon activation with solid phase anti CD 3 mAb . gamma delta TCR+ DN thymocytes produced IL 2 , IFN gamma and TNF alpha , whereas alpha beta TCR+ DN thymocytes produced IL 4 , IFN gamma , and TNF alpha but not IL 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma is a potent activator of monocytic cell functions , including the stimulation of TNF alpha and the control of IL 6 synthesis . ^^^ In this study , we addressed the question of whether or not TNF alpha and IL 6 play a role in modulating of IFN gamma binding . ^^^ The number of IFN gamma R per cell rose by three and fourfold after 16 h of treatment with TNF alpha and IL 6 , respectively . ^^^ Scatchard analysis of binding data showed that neither TNF alpha nor IL 6 affected the affinity of IFN gamma for its receptor . ^^^ The increased surface expression of IFN gamma R induced by TNF alpha and IL 6 correlated with the rise in IFN gamma mRNA receptor levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro studies suggest that TNF alpha and IFN gamma regulate thymocyte proliferation , but little evidence exists for the constitutive production of these cytokines in normal human thymus . ^^^ In paired experiments , we examined frozen sections of postnatal human thymus from four control children and four age matched children with Down syndrome ( DS ) ( trisomy 21 ) for TNF alpha and IFN gamma mRNA expression using in situ hybridization . ^^^ Our results demonstrate , for the first time , the constitutive production and location of TNF alpha and IFN gamma in postnatal human thymus . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF production in short term cultures of peritoneal macrophages from DP rats was significantly less than that from control DR rats , both in the basal state and after stimulation with either interferon gamma ( IFN gamma ) or lipopolysaccharide ( LPS ) in vivo and in vitro . ^^^ In contrast , TNF production by macrophages from CFA injected DP rats ( basal and IFN gamma or LPS stimulated ) was equal to or greater than that by macrophages from DP rats and similar to TNF production by macrophages from CFA injected DR rats . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin 1 ( IL 1 beta ) , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) were measured in serum from children with measles using an immunoradiometric assay . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
By using this method , mRNAs for tumor necrosis factor alpha , interleukin 1 alpha ( IL 1 alpha ) , IL 2 , IL 4 , IL 5 , and IFN gamma were detected in RNA from the spleen cells of uninfected mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Organs were removed at 30 min , and 1 , 2 , 6 , and 24 h after primary and secondary infections , and PCR 3 assisted messenger RNA ( mRNA ) amplification was performed on matched samples using primers specific for IL 1 beta , IL 6 , M CSF , GM CSF , TNF alpha , IFN gamma , IL 10 , IL 4 , IL 2 , IL 3 and I 1 2Rp55 . ^^^ Using competitive PCR for semiquantitative determination of mRNA levels , the amount of IL 1 beta and IL 6 mRNA was found to be very similar during primary and secondary infection , whereas TNF alpha mRNA was found to be increased by approximately 10 fold 2 h and IFN gamma mRNA by approximately 50 to 100 fold 6 h after reinfection when compared with a primary challenge . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To examine the regulation of cytokine synthesis during murine listeriosis , we have monitored IFN gamma , TNF alpha , and IL 1 beta mRNA levels in the spleens of C57B1 / 6 mice after the i . v . infusion of virulent and nonvirulent preparations of Listeria monocytogenes ( LM ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A synthetic peptide homologous to retroviral envelope protein down regulates TNF alpha and IFN gamma mRNA expression . ^^^ These results suggest that CKS 17 down regulates both TNF alpha and IFN gamma production at mRNA level . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the 4 day post induction supernatants the levels of IFN gamma , IL 1 alpha , IL 2 , and TNF alpha were measured by a sensitive immunoassay . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Enhanced production of TNF alpha was not seen after stimulation with other cytokines such as IL 2 or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Hemorrhage reduced ( P less than 0 . 05 ) SPL proliferation by 60 % , SPL release of interleukin 2 ( IL 2 ) by 47 % , interferon gamma ( IFN gamma ) by 67 % , TNF by 54 % , and interleukin 6 ( IL 6 ) by 46 % compared to sham . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although L . donovani also failed to stimulate the release of tumor necrosis factor , an important macrophage derived cofactor for IFN gamma secretion by NK cells , exogenous recombinant tumor necrosis factor alpha could not restore the IFN gamma response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We characterized the mechanisms by which recombinant ( r ) tumor necrosis factor ( TNF ) , IFN gamma , and IL 1 , alone and in combination , regulate human lung fibroblast hyaluronic acid ( HA ) production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation of Fc epsilon RII expression on monocytes was shown to be an activity of IL 4 ( 24 . 5 + / 5 . 9 % ) , granulocyte macrophage CSF ( 28 . 1 + / 5 . 2 % ) , IFN alpha ( 15 . 8 + / 5 . 3 % ) , IFN gamma ( 10 . 4 + / 3 . 7 % ) , and macrophage CSF ( 7 . 3 + / 0 . 7 % ) but not of IL 2 , IL 6 , or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) released in acute viral hepatitis , acts in synergy with TNF alpha . ^^^ HL 60 , KG 1 , and U 937 AML cells treated 3 , 6 , and 9 days with physiologically attainable TNF alpha ( 10 U / ml ) , IFN gamma ( 100 U / ml ) and LPS ( 10 ng / ml ) levels , have significantly diminished viability and cell growth versus controls . ^^^ Treatment of HL 60 AML cells with LPS / TNF alpha / IFN gamma also resulted in significantly increased monocytic pathway differentiation not seen with KG 1 or U 937 AML cells . ^^^ HL 60 AML cells treated with TNF alpha / IFN gamma for 6 days released endogenous TNF alpha ( 1 . 57 U / 10 ( 6 ) cells ) upon LPS stimulation compared to less than 0 . 01 U / 10 ( 6 ) cells in non LPS stimulated TNF alpha / IFN gamma treated cells or untreated cells ( p less than 0 . 0001 ) . ^^^ Untreated HL 60 AML cells co cultured with HL 60 cells pretreated for 6 days with TNF alpha / IFN gamma and then subjected to LPS stimulation had significantly diminished cell growth compared to controls ( p less than 0 . 0001 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The influence of recombinant interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF ) on IL 4 induced proliferation of postnatal human thymocytes from eight children with Down syndrome ( DS , trisomy 21 ) and 18 control children was evaluated . ^^^ Both IFN gamma and TNF inhibited IL 4 driven proliferation in a dose dependent manner , but DS thymocytes were found to be significantly more sensitive to inhibition by both cytokines . ^^^ Our studies suggest an important role for IL 4 in the proliferation of T cell receptor gamma , delta+ thymocytes and demonstrate regulatory functions for IFN gamma and TNF in human thymocyte proliferation . ^^^ The increased sensitivity of DS thymocytes to IFN gamma and TNF may explain anatomical abnormalities in DS thymuses and suggests the involvement of genes encoded on human chromosome 21 in the responses to both IFN gamma and TNF . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Earlier we showed that TNF induced stimulation of IL 8 mRNA accumulation in human FS 4 fibroblasts was inhibited by interferon beta ( IFN beta ) or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , at least two cytokines ( i . e . , TNF alpha and IFN gamma ) are released during these interactions . ^^^ Besides playing a role in the pathway of T cell immunosuppression , TNF alpha and IFN gamma could also contribute to immunopathological features that occur during trypanosome infections . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study with cloned NK cells or NK cell lines from HIV infected individuals showed : ( 1 ) a decrease in their lytic capability against target cell lines ; ( 2 ) a low ability to form conjugates with K 562 and U 937 cell lines with respect to controls ; ( 3 ) a decreased ability to kill bound target cells ; ( 4 ) low levels of released NKCF , TNF alpha and IFN gamma after incubation with U 937 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
DESIGN : The effects of the cytokines , interferon alpha ( IFN alpha ) , interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) on steroidogenesis by human luteal cells were examined in vitro . ^^^ The combination of IFN gamma and low doses of TNF alpha induced a further significant inhibition , whereas there was no effect of TNF alpha alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although IFN gamma / LPS were able to stimulate production and secretion of TNF and IL 6 in the nonadherent cells , without cell matrix interaction , the process was significantly delayed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Modulation of IL 2 , IFN gamma , TNF alpha and IL 4 production in mice of different ages by thymopentin . ^^^ The effect of an immunomodulator drug thymopentin ( TP 5 ) on the production of various cytokines ( IFN gamma , IL 2 , IL 4 , TNF alpha ) in mice of different ages has been studied . ^^^ TP 5 enhanced IL 2 , TNF alpha and IFN gamma production but reduced the IL 4 secretion by splenocytes from aged mice ( greater than 120 week old ) in vitro . ^^^ However , it had no effect on the IL 2 , IFN gamma , TNF alpha or IL 4 production by splenocytes from young and adult mice . ^^^ The TP 5 effect on TNF alpha and IFN gamma was similar , even though it was significant only in old mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In order to diminish the presence of these immune suppressor cells , LLC bearing mice were treated with low doses of gamma interferon ( IFN gamma ) ( 100 units / mouse ) plus TNF alpha ( 10 units / mouse ) . ^^^ Treatment of LLC bearing mice with these low doses of IFN gamma plus TNF alpha diminished the suppressive activity of their bone marrow cells , as measured by the effect on normal macrophage activation to secrete TNF alpha . ^^^ In in vivo adoptive transfer studies , bone marrow from placebo treated LLC bearers stimulated tumor establishment and metastasis , while the bone marrow of IFN gamma plus TNF alpha treated tumor bearers diminished LLC establishment and metastasis . ^^^ The effect of the low dose treatments with IFN gamma and / or TNF alpha on the recurrence of excised s . c . tumors was also assessed . ^^^ Treatment of mice following tumor excision with either IFN gamma , TNF alpha , or the combination of IFN gamma plus TNF alpha reduced recurrence . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma and TNF alpha are induced upon CD 3 but not CD 2 stimulation , both in the presence and absence of IL 2 . ^^^ Interestingly , the B67 . 1 mAb amplifies the OKT 3 induced responses by 2 to 10 fold , bringing the IFN gamma and TNF alpha levels of production up to 200 U / ml . ^^^ Of all the tumor cell lines tested as inducers , only K 562 cells are able to stimulate the production of IFN gamma and TNF alpha in TALL 103 / 2 and TALL 104 cells , especially upon culture in IL 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using both chemical measurement of cellular and supernatant GAGS and 35S labelling to identify newly synthesised GAGS , interleukin 1 ( IL 1 ) , tumour necrosis factor ( TNF ) and interferon gamma ( IFN gamma ) were shown to influence sulphated GAG metabolism significantly . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 4 also selectively decreased the ability of IFN gamma and / or IL 2 to augment TNF alpha mRNA expression in ANA 1 macrophages . ^^^ These results indicate that IFN gamma plus IL 2 induced tumoricidal activity is dependent upon the metabolism of L arginine to reactive nitrogen intermediates , and they establish a role for TNF alpha as a required intermediate for IL 2 dependent NO 2 production and tumoricidal activity . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study we demonstrate that supernatants of highly enriched cultured Langerhans cells ( cLC ) display IL 1 , IL 6 , granulocyte / macrophage ( GM ) CSF , and TNF alpha , but no IL 2 , IL 3 , IL 4 , and IFN gamma activities . ^^^ Polymerase chain reaction analysis of cLC and cKC revealed that IL 1 beta and IL 6 transcripts are virtually limited to cLC , whereas IL 1 alpha , GM CSF , and TNF alpha messages are preferentially exhibited by cKC . mRNA coding for IL 2 , IL 3 , IL 4 , and IFN gamma could neither be amplified from cLC nor from cKC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The inhibitor has no cross reactivity against a panel of known cytokines ( IL 1 alpha , IL 1 beta , IL 2 , sIL 2R , IL 6 , tumor necrosis factor ( TNF ) , interferon gamma ( IFN gamma ) ) and is distinct from the IL 1 receptor antagonist ( IL 1ra ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Concentrations of TNF beta , interleukin 1 alpha ( IL 1 alpha ) , IL beta , IL 2 , IL 3 , IL 6 or interferon gamma ( IFN gamma ) up to 5 ng / ml failed to show any cross reactivity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of lipopolysaccharide ( LPS ) , recombinant human tumor necrosis factor alpha ( TNF ) , recombinant human interleukin 1 beta ( IL 1 beta ) , and interferon gamma ( IFN gamma ) on IL 6 production were determined by enzyme linked immunosorbent assay ( ELISA ) and by Northern blot analysis in cultured human dermal microvascular endothelial cells ( HDMEC ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This article summarizes the effects of granulocyte macrophage / colony stimulating factor ( GM CSF ) , interleukin 1 alpha ( IL 1 alpha ) , tumor necrosis factor alpha ( TNF alpha ) , transforming growth factor beta ( TGF beta ) , and interferon gamma ( IFN gamma ) on the modulation of antigen presentation by epidermal APC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
All three variants were IFN reactive with regard to induction of 2 ' , 5 ' oligoadenylate ( 2 5A ) synthetase activity and were similarly sensitive to the growth inhibiting action of IFN gamma and tumor necrosis factor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The quantitative relationships among the in vitro lymphocyte proliferation in peripheral blood in 19 healthy donors to purified protein derivative ( PPD ) and the killed Mycobacterium tuberculosis , interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) production in these culture supernatants , and the in vivo skin reaction to PPD which were simultaneously measured were studied . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine ( IFN alpha , IFN gamma , IL 1 alpha , TNF alpha ) induced modulation of HLA cell surface expression in human breast cancer cell lines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The activating effect of LPS , IFN gamma , IL 2 , TPA , TNF and Zymosan was assessed by the determination of the activities of alpha naphthyl butyrate esterase ( ANBE ) , tartrate resistant acid phosphatase ( TRAP ) , beta glucuronidase ( BG ) and 5 ' nucleotidase ( 5 ' NT ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After 2 h of stimulation the mRNA for interleukin 1 alpha ( IL 1 alpha ) , interleukin 2 ( IL 2 ) , interleukin 3 ( IL 3 ) , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) were detectable and remained present during the whole time period studied ( 22 h ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To determine whether interleukin 1 alpha ( IL 1 alpha ) , IL 1 beta , IL 2 , IL 4 , interferon gamma ( IFN gamma ) , IL 6 , and tumor necrosis factor alpha ( TNF alpha ) are detected more frequently in sera from scleroderma patients than in sera from controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In an attempt to establish whether the putative ICSA autoantigen is present in the surface of human islet cells in the diabetic pancreas , the insulitis microenvironment was emulated by exposing the islets to three types of stress : 1 ) cytokines ( IFN gamma and TNF alpha ) ; 2 ) heat shock ; and 3 ) hyperglycemia . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Spontaneous and lipopolysaccharide or mitogen induced production of the cytokines , TNF alpha , IL 1 beta , and IFN gamma , by peripheral blood mononuclear cells ( PBMNC ) of these SSc patients was measured by immunoassays . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These results suggest that toxin induced monocyte TNF alpha production is upregulated by IFN gamma , which likely represents the T cell requirement in SE mediated weight loss . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha and IFN gamma are central to this process , and mycobacterial polysaccharides may be their main determinant . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of the cytokines IFN gamma and TNF alpha on epithelial HLA class 2 expressions have been studied in adult human jejunal mucosal biopsy specimens cultured in vitro . ^^^ Specimens from nine patients with normal jejunal histology were cultured for 24 h with IFN gamma , TNF alpha , or both . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma and TNF alpha are most likely involved as the expression patterns can be reproduced with these cytokines in vitro on colonic epithelial cell lines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Subsequently , we confirmed the presence of T cell factors in the supernatants responsible for the observed effects : interferon gamma ( IFN gamma ) and tumour necrosis factor ( TNF ) . ^^^ Experiments with combinations of recombinant mouse IFN gamma and human lymphotoxin or TNF alpha showed that these lymphokines could substitute for the direct T lymphocyte effects causing a synergistic growth inhibition and PGE 2 release from mouse MC . ^^^ Pointing to the essential role of T helper cell specific products , IFN gamma antibodies abolished both the IFN gamma and the combined IFN gamma / TNF alpha effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using immunohistochemical techniques , the presence of IL 1 alpha , IL 1 beta , IL 6 , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) in the skin in tuberculin purified protein derivative ( PPD ) induced DTH reactions was investigated in six normal individuals . ^^^ Double immunofluorescence and staining on sequential sections showed that IFN gamma staining cells were CD3+ T cells ; TNF alpha , IL 1 and IL 6 staining cells were mainly of the CD68+ macrophages / monocytes and that 80 % of the CD1a+ cells ( Langerhans like cells ) in the dermis contained TNF alpha and IL 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Natural cell mediated cytotoxicity ( CMC ) , spontaneous release of tumor necrosis factor ( TNF alpha ) by mononuclear cells in vitro , and PHA induced interferon ( IFN gamma ) production were examined by standard methods in 14 females with anorexia nervosa ( AN ) and marked weight loss and in 16 normal matched controls . ^^^ In the anorectic patients there was a significant increase in TNF production ( 16 + / 5 vs 4 + / 3 mu / ml ) , and depressed CMC ( 4 + / 2 vs 10 + / 3 lytic units per 50 / 10 ( 6 ) cells ) and IFN gamma production ( 172 . 5 + / 25 vs 367 + / 34 mu / ml ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Primary rat astrocytes express TNF alpha protein in response to various stimuli including a combined treatment with IFN gamma and LPS , or IFN gamma and IL 1 beta . ^^^ This study was undertaken to further elucidate the mechanisms underlying TNF alpha gene expression in the astrocyte , and to determine the intracellular signaling pathways involved in IFN gamma / LPS and / or IFN gamma / IL 1 beta induction of the TNF alpha gene . ^^^ IFN gamma / LPS induces transcriptional activation of the TNF alpha gene as assessed by nuclear run on experiments . ^^^ Two protein kinase C ( PKC ) inhibitors , H 7 and staurosporine , abrogate IFN gamma / LPS and IFN gamma / IL 1 beta induced TNF alpha expression in a dose dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It is shown that IL 2 cultured fetal TcR 5 gamma 3 thymocytes were capable of producing IL 3 , GM CSF , TNF alpha and IFN gamma upon TcR triggering . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Some of these compounds were compared to flavone acetic acid ( FAA ) in their ability to induce cytokines as well as to mediate antitumor effects against murine renal cancer ( Renca ) and a mouse colon cancer ( MCA 38 ) . 5 Methyl XAA and 5 chloro XAA proved to be more potent than FAA on a mg / kg basis for induction of the genes for IFN alpha , IFN gamma , and TNF alpha , and for IFN and TNF activities in the sera of treated mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NK cell precursors were also cultured with a combination of IL 2 and interferon gamma ( IFN gamma ) or IL 2 and tumor necrosis factor alpha ( TNF alpha ) , but no CM . ^^^ Results show that IFN gamma and TNF alpha were able to substitute CM . ^^^ The addition of anti IFN gamma or anti TNF alpha antibodies to LTBMC cells , cultured in the presence of IL 2 and CM , inhibited cytotoxicity induction in a dose dependent manner . ^^^ The data indicate that IFN gamma and TNF alpha production may be required for IL 2 induction of NK activity , and are consistent with the hypothesis that NK generation involves collaboration between IL 2 and other bone marrow microenvironmental growth factors . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Effect of tuftsin and its retro inverso analogue on the release of interferon ( IFN gamma ) and tumor necrosis factor ( TNF alpha ) by human leucocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Because tumor necrosis factor ( TNF ) and procoagulant activity ( PCA ) elaboration by the tissue fixed macrophage play a central role in the host response to endotoxin , we asked whether the paradoxical effects of IFN gamma may be caused by priming of the macrophage for TNF and / or PCA production . ^^^ In vitro , IFN gamma produces a marked augmentation in TNF but does not alter PCA elaboration in response to endotoxin , demonstrating the selectivity of IFN gamma priming of the macrophage . ^^^ In vivo , IFN gamma pretreatment followed by an established subclinical endotoxin exposure enhances toxicity while simultaneously increasing peak serum TNF levels . ^^^ Because this response is also dependent on the host ' s underlying immune state , IFN gamma treatment in the immunocompetent host has the potential to produce deleterious effects by eliciting an exaggerated TNF response during endotoxemia . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Granulocyte macrophage colony stimulating factor ( GM CSF ) stimulated macrophages had a 3 5 fold reduction in apo E secretion , comparable to that observed for gamma interferon ( IFN gamma ) , while tumor necrosis factor alpha ( TNF alpha ) and interleukin 1 beta ( IL 1 beta ) resulted in a 2 fold decrease . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effect of interleukin 1 beta ( IL 1 beta ) , interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) and lipopolysaccharide ( LPS ) on the expression of the C 2 and C 3 genes in human adherent monocytes was studied . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In order to identify possible endogenous mediators of these responses , we used highly purified preparations of recombinant murine ( rMu ) cytokines and neutralizing cytokine specific monoclonal antibodies ( MAbs ) to examine whether interferon gamma ( IFN gamma ) and / or tumor necrosis factor ( TNF ) down regulate macrophage cyclooxygenase activity in vivo . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : The study was performed with a panel of antibodies as markers of inflammatory cells , adhesion molecules , and cytokines ( interferon gamma [ IFN gamma ] , tumor necrosis factor alpha [ TNF alpha ] and interleukins 1 alpha , 1 beta , and 8 [ IL 1 alpha , IL 1 beta , and IL 8 , respectively ] ) . ^^^ The number of cells expressing IFN gamma and TNF alpha , assumed to be the products of the helper T cell ( TH ) 1 subset , was unaltered despite the impressive clinical benefit observed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The levels of interleukin 1 alpha ( IL 1 alpha ) , interleukin 2 ( IL 2 ) , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) in supernatants of the cultures were determined ( irradiated either before or after stimulation ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Also supernatants collected from cisplatin or IFN gamma treated monocytes demonstrated enhanced cytotoxicity against actinomycin D treated L 929 cells , suggesting that cisplatin or rIFN gamma treated monocytes release tumor necrosis factor ( TNF ) into the culture medium . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pretreatment of alveolar macrophages ( AM phi ) with interferon gamma ( IFN gamma ) synergizes with P . carinii to produce increased levels of TNF , a condition which pentamidine was also able to antagonize . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
On the contrary , other mitogens such as PHA or Con A , and cytokines as IFN gamma , IL 2 , M CSF , TNF alpha and IL 1 beta showed no influence on the IL 2R expression on monocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The potential for additive or synergistic augmentation of NK cell activity of nMNC with recombinant tumor necrosis factor ( TNF alpha ) , interleukin 1 ( IL 1 ) , interferon gamma ( IFN gamma ) and cisplatin in different combinations was examined . ^^^ Further , only a moderate augmentation of NK activity was observed when nMNC were treated with cisplatin plus TNF or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Production of IL 4 , IL 2 , IFN gamma , and TNF alpha by peripheral blood mononuclear cells of patients with atopic dermatitis . ^^^ We examined the production of IL 4 , IL 2 , IFN gamma and TNF alpha by PBMC of AD patients and non AD controls . ^^^ Although the production of IL 2 and TNF alpha by AD patients was significantly lower than that of non AD controls , the production of IL 4 and IFN gamma did not show significant differences between AD and non AD individuals . ^^^ There was significant positive correlation between IL 4 and IL 2 production , and between IFN gamma and TNF alpha production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Direct stimulation of cytokines ( IL 1 beta , TNF alpha , IL 6 , IL 2 , IFN gamma and GM CSF ) in whole blood . 1 . ^^^ Production of interleukin 1 beta ( IL 1 beta ) , interleukin 6 ( IL 6 ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 2 ( IL 2 ) , interferon gamma ( IFN gamma ) and granulocyte macrophage colony stimulating factor ( GM CSF ) after stimulation by lipopolysaccharide ( LPS ) and phytohemagglutinin ( PHA ) was studied in 1 / 10 diluted whole blood ( WB ) culture and in peripheral blood mononuclear cell ( PBMC ) culture . ^^^ Cytokines IL 1 beta , TNF alpha and IL 6 are preferentially stimulated by LPS whereas IL 2 , IFN gamma and GM CSF are stimulated by PHA . ^^^ In contrast , production levels of TNF alpha and IFN gamma are higher in WB than in PBMC whereas production levels of IL 6 and IL 2 are lower in WB than in PBMC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1 alpha , IL 3 , IL 4 , IL 6 , IFN gamma , TNF alpha and GM CSF used either alone or in combination with IL 2 , were investigated in this study . ^^^ However , addition of TNF alpha , or IFN gamma to IL 2 in cultures resulted in a significant suppression of LAK cell activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
For this purpose , we examined the effects of recombinant interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) on the human colonic goblet cell line Cl . 16E by morphological and kinetic studies , and by the assessment of mucus production during IFN gamma / TNF alpha treatment . ^^^ The combined action of IFN gamma and TNF alpha caused a dose related cellular exfoliation , leading to the formation of a mucoid cap made of mucus and cellular debris . ^^^ Furthermore , 5 aminosalicylic acid partially protected Cl . 16E cells against cellular injury caused by IFN gamma and TNF alpha . ^^^ On the whole , these morphological and kinetic findings argue that the changes induced in Cl . 16E cells by IFN gamma and TNF alpha closely parallel those observed during the acute phase of ulcerative colitis , and show that these cytokines can regulate intestinal mucus production by modulating cellular exfoliation , thus leading probably to a reinforced protection of the damaged mucosa . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Erythrogenic toxins and low molecular weight mitogens , inducing cytokines ( IL 2 , IL 3 , IL 6 , TNF alpha , IFN gamma ) seem to be involved in the pathogenesis of these severe infections . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CSF 1 and IL 1 were found to enhance the tumoricidal activity of LAM in a dose dependent manner , whereas IFN gamma and TNF had inhibitory effects . ^^^ It is suggested from these results that the cytotoxicity of LAMs is regulated by CSF 1 , IL 1 , IFN gamma , and TNF , and that the production of cytotoxic molecules is involved in cell mediated killing by LAMs . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This review describes the studies in which the uveitogenic properties of several cytokines , including tumor necrosis factor ( TNF ) , interleukin 1 ( IL 1 ) , IL 6 , IL 8 and interferon gamma ( IFN gamma ) , were investigated and the reports on intraocular expression of cytokines , such as TNF , IL 2 , IL 6 and IFN gamma , during uveitis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have also demonstrated that gamma interferon ( IFN gamma ) enhances TNF alpha release from LPS stimulated macrophages but not from phorbol myristate acetate stimulated cells . ^^^ Addition of IFN gamma to macrophages either during the initial stimulation or during a second stimulation with LPS enhances levels of TNF alpha released after the second LPS challenge . ^^^ The greatest enhancement is observed when IFN gamma is added during both exposures to LPS , but addition of IFN gamma during only the initial LPS stimulation still results in marked enhancement of TNF alpha release in response to a second stimulation with LPS 24 h later . ^^^ If an interval of 2 days of culture in medium alone separates the first and second 24 h LPS stimulations , IFN gamma enhances TNF alpha release only when it is included during the second LPS exposure , indicating that , unlike the persistence of endotoxin tolerance , enhancement of TNF alpha release by IFN gamma is transient . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Northern blot analysis of total RNA , obtained from purified PMN stimulated overnight with LPS or cytokines ( IFN gamma , TNF alpha , and IL 1 ) showed the 5 . 3 kb RNA transcript reported for C 3 in hepatocytes and monocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In reversal reactions , IL 1 beta , TNF alpha , IL 2 , and IFN gamma mRNA were prominent and found to increase during the reaction , concomitant with decreases in expression of mRNA for IL 4 , IL 5 , and IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum concentrations of interleukin 2 ( IL 2 ) , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , interleukin 6 ( IL 6 ) , interleukin 1 ( IL 1 ) and interferon alpha ( IFN alpha ) were determined by commercially available enzyme linked immunosorbent assay ( ELISA ) or radioimmunoassay ( RIA ) in cancer patients treated with recombinant IL 2 ( rIL 2 ) either as 1 h infusion ( 3 or 5 10 10 ( 6 ) / m2 ) or continuous intravenous infusion for 5 days ( 3 10 10 ( 6 ) / m2 / day ) . ^^^ One hour infusion of IL 2 induced a very rapid secretion of TNF alpha , IL 6 and IFN gamma with considerably higher peak levels than during IL 2 continuous intravenous infusion . ^^^ Following IL 2 1 h infusion , rising TNF alpha serum levels preceded the increase of serum IFN gamma or IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study was undertaken to determine the production of tumor necrosis factor ( TNF ) and interferon gamma ( IFN gamma ) by biological response modifier ( BRM ) in patients with renal cell carcinoma ( RCC ) . ^^^ Peripheral blood mononuclear cells ( PBMC ) , which were donated from thirteen patients with RCC and five healthy controls , were cultured with streptococcal preparation , OK 432 , and / or macrophage colony stimulating factor ( M CSF ) , and the TNF levels and IFN gamma levels in the supernatant were evaluated . ^^^ TNF activities were assayed by cytotoxicity to L 929 cells and IFN gamma activities were measured by inhibition of the cytopathic effects of sindbis virus on FL cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The potential role of tumor necrosis factor alpha ( TNF alpha ) , interferon alpha ( IFN alpha ) and interferon gamma ( IFN gamma ) in the therapy of non lymphoid leukemia was studied in ten non lymphoid leukemia cell lines . ^^^ Our data thus suggest that TNF alpha , IFN alpha and IFN gamma together have a potential role in the immunotherapy of non lymphoid leukemia in terms of their antiproliferative action , and their ability to induce differentiation and to modulate drug sensitivity . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In PBC , diminished synthesis of lymphotoxin ( TNF beta ) , tumor necrosis factor ( TNF alpha ) and interferon gamma ( IFN gamma ) was found both in T cell lines from liver tissue and in peripheral blood . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Because circulating serum levels of IFN gamma can be detected soon after TNF alpha and IL 1 in response to endotoxin , we investigated the role of IFN gamma in endotoxin and TNF alpha lethality . ^^^ Specific neutralizing antibodies to murine TNF alpha ( anti TNF alpha Ab ) or murine IFN gamma ( anti IFN gamma Ab ) produced in our laboratory protected mice against the lethality of Escherichia coli endotoxin ( LPS ) administered 6 h later . ^^^ Doses of TNF alpha ( 300 micrograms / kg ) and IFN gamma ( 50 , 000 U ) which were well tolerated when given individually were synergistically lethal in combination ( 0 % lethality vs 100 % lethality , P 2 less than 0 . 001 ) , and were associated with higher serum levels of IL 6 than with either cytokine alone . ^^^ We conclude that IFN gamma is synergistic with TNF alpha , is essential for the lethality of LPS and TNF alpha , and may have modulating effects on the negative control of serum levels of TNF alpha after LPS in mice . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A neutralization test using agents against interferon gamma ( IFN gamma ) , tumor necrosis factor ( TNF ) , and lymphotoxin ( LT ) showed that antiproliferative activity was due to IFN gamma , but not to TNF or LT . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The combined addition of tumour necrosis factor alpha ( TNF alpha ) with interferon gamma ( IFN gamma ) similarly increased the dependency for cellular energy provision from an oxidative to the glycolytic state . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Peripheral blood mononuclear cell ( PBMC ) fractions from both groups were assayed for production of interleukin 1 ( IL 1 ) , interleukin 2 ( IL 2 ) , interleukin 6 ( IL 6 ) , alpha interferon ( IFN alpha ) , beta interferon ( IFN beta ) , gamma interferon ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and tumor necrosis factor beta ( TNF beta ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neutralizing antibodies to TNF alpha and IFN gamma inhibited upregulation of EGF / TGF alpha receptors and TGF alpha by the respective cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To determine safety and efficacy of tumor necrosis factor ( TNF ) and interferon gamma ( IFN gamma ) in the treatment of patients with acquired immunodeficiency syndrome ( AIDS ) related complex , a randomized , double blind study was conducted . ^^^ Patients were administered tumor necrosis factor ( TNF ) ( 10 micrograms / m2 ) or IFN gamma ( 10 micrograms / m2 ) , or both intramuscularly three times weekly for 16 weeks . ^^^ TNF and IFN gamma were tolerable after premedication with acetaminophen ; however , no significant change in markers of human immunodeficiency virus infection was demonstrated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Peripheral blood mononuclear cells ( PBMC ) from untreated patients produced IL 1 beta , tumour necrosis factor alpha ( TNF alpha ) and IL 6 in response to mitogenic stimulation with phytohaemagglutinin ( PHA ) , only low levels of IL 1 beta , IL 2 and TNF alpha in response to OvAg , but higher amounts of IL 4 and interferon gamma ( IFN gamma ) in response to OvAg than control individuals . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The continual presence of IFN gamma and TNF alpha , cytokines with reported anti toxoplasmic activity , in the CNS of B 10 mice throughout the latter half of the experimental period did not diminish the severity of infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In THP 1 cells , IFN gamma induces cell surface expression of HLA DR and CD 54 and production of IL 1 beta , TNF alpha , and IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , the CSF granulocyte macrophage ( GM ) CSF and IL 3 as well as TNF alpha , while inactive by themselves , were synergistic with LPS and IFN gamma in inducing nitric oxide production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This influx was not mimicked by the inoculation of recombinant interferon gamma ( IFN gamma ) or tumor necrosis factor alpha ( TNF alpha ) . ^^^ The antigen induced recruitment of neutrophils was not affected by coinoculation of anti IFN gamma antibodies , was enhanced by anti TNF alpha antisera , and was significantly reduced by antisera against macrophage inflammatory proteins MIP 1 and MIP 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this regard , IL 2 infusion caused a very rapid rise of tumour necrosis factor alpha ( TNF alpha ) plasma concentration , whereas other cytokines , such as interferon gamma ( IFN gamma ) , were induced only at later times . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Anti IFN gamma treatment resulted in decreased levels of TNF alpha in the BAL of F . rectivirgula after 4 weeks of treatment ( 56 U / ml , P less than 0 . 01 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , HIV expression appeared to alter the regulation of genes involved in proliferation and functional differentiation such as a decreased expression of IL 2 and IL 2R alpha and an increased expression of IFN gamma and TNF alpha mRNA . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of TNF alpha mRNA in macrophages stimulated with IFN gamma , IL 2 , or the combination is markedly suppressed by IL 4 whereas LPS induced TNF alpha mRNA is unaffected . ^^^ The IL 4 mediated suppression of IFN gamma / IL 2 driven TNF alpha gene expression appears to be mediated at the level of transcription . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inflammatory mouse peritoneal macrophages were activated by IFN gamma in synergy with IL 2 or Lipid A to mediate TNF production for autocrine generation of cytotoxic nitric oxide ( NO ) to kill P 815 or L 1210 tumor targets . ^^^ It was determined that for IL 2 , but not Lipid A , to effectively trigger activation of IFN gamma primed macrophages , the tumor targets must be also present for interaction with effector macrophages to mediate the production of TNF and NO . ^^^ IFN gamma and IL 2 activated macrophages from syngeneic DBA / 2 and allogeneic C3H mice had identical MHC unrestricted requirements for interaction with DBA / 2 mouse derived P 815 and L 1210 targets to mediate production of TNF and NO for tumor cytotoxicity . ^^^ This was confirmed by experiments in which 24 h cell free culture fluids , derived from either P 815 or L 1210 tumor targets , substituted for the intact tumor cells in the stimulation of TNF mRNA synthesis and secretion with NO generation of TNF mRNA synthesis and secretion with NO generation by IFN gamma and IL 2 activated C3H or DBA / 2 macrophages . ^^^ The activity in 24 h culture fluids derived from P 815 and L 1210 tumor targets was tentatively designated as tumor derived recognition factor ( s ) ( TDRF ) since it was produced constitutively by the tumor targets and synergized with IFN gamma and IL 2 to induce macrophage production of TNF and NO for death of the same targets . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
T cell activation potency of all three Hu OKT 3 Ab was assessed by proliferation , induction of activation marker expression ( IL 2R and Leu 23 ) , and lymphokine production ( TNF alpha and IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Several cytokine mRNA such as those coding for IL 5 , IL 6 , IFN gamma and also the IL 4 receptor were found to be up regulated in IL 4 transgenic mice , whereas IL 1 , IL 2 , IL 3 , TNF and LT mRNA levels did not seemed to be influenced by IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In parallel , we observed a marked reduction in the T cell activation triggered by the mAb ( proliferation , cell surface expression of early activation markers including Leu 23 and IL 2R , and release of TNF alpha , IFN gamma , and granulocyte macrophage CSF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Contact with the tumor cells stimulated NK cells to proliferate , secrete IFN gamma , TNF alpha , and soluble IL 2R , up regulate cell surface expression of IL2R p 55 and p 75 as well as CD 16 Ag , and mediate higher levels of antitumor activity in 51Cr release assays . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Such suppressor but not control supernatants increased the production of IL 4 and IL 6 whilst suppressing IL 1 beta , TNF alpha , IL 2 and IFN gamma production by monocytes and lymphocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In all these effects TNF interacts positively with interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In particular , IL 1 ( alpha and beta ) , IL 2 , TNF ( alpha and beta ) , and IFN gamma mRNA were readily detected , whereas IL 3 , IL 4 , IL 5 , IL 6 , IL 7 , and granulocyte macrophage colony stimulating factor mRNA were not detected , or were detectable only at very low levels . ^^^ The effect of in vitro exposure of these cells to the immunizing Ag was also investigated ; again , IL 1 , IL 2 , TNF , and IFN gamma mRNA were abundant , but in addition , IL 3 , IL 6 , and granulocyte macrophage colony stimulating factor mRNA were greatly increased , suggesting an important role in the recall response . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using neutralizing Abs , we noticed that the T cell derived enhancing activity is entirely ascribed to the combined effects of IFN gamma ( potent inhibitor of CD 23 on normal B cells ) , TNF alpha ( which has no effect on normal B cells ) , and IL 2 ( which has a slight enhancing effect on both CLL and normal B cells ) . ^^^ Furthermore , recombinant IFN gamma as well as IFN alpha , TNF alpha , and IL 2 ( but not IL 3 , IL 5 , IL 6 , IL 7 , and lymphotoxin ) significantly enhance CD 23 protein and mRNA expression on B CLLs , in the presence or absence of IL 4 . ^^^ Inasmuch as optimal CD 23 expression absolutely requires the combination of IFN gamma , IL 2 , TNF alpha ( the production of which is increased in CLL disease ) , and IL 4 , it was relevant to show that IL 4 mRNA is indeed expressed in fresh T CLL cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
About 40 % of the double negative T ( DNT ) cells bore TcR alpha beta , and in vitro clones of the same phenotype , established from the i . t . challenged lungs , were shown , upon ConA stimulation , to synthesize mRNA for IL 2 , IL 3 , IL 4 , IL 5 , IL 6 , IFN gamma , TNF alpha , GM CSF , TGF beta but not of IL 1 or EGF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Isolated rat brain microglia display enhanced expression of Fc receptors on treatment with interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) , interleukin 1 ( IL 1 ) and lipopolysaccharide ( LPS ) , whereas major histocompatibility complex ( MHC ) antigen expression is enhanced only by IFN gamma . ^^^ Although TNF and LPS individually have no effect on MHC expression by microglia , they both antagonize IFN gamma induced expression . ^^^ The enhanced expression of Fc receptors observed in the presence of IFN gamma , TNF or LPS is significantly inhibited by the combination of IFN gamma with either LPS or TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma , TNF alpha , IL 1 , EGF and TGF beta also exerted a synergistic effect on proliferation inhibition in certain cell lines , whereas G CSF was inactive . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Analysis of the supernatant culture medium after 72 h incubation of PBMC and their highly purified subpopulations demonstrated that CySF L 2 induced release of IFN gamma from CD3+T cells and CD56+CD3 NK cells and of TNF alpha and prostaglandin E 2 from monocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To study the local immunological effects of intravesical bacillus Calmette Gurin ( BCG ) therapy in superficial bladder cancer patients , the production of interleukin 1 ( IL 1 ) , IL 2 , IL 6 , tumour necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) was investigated in the urine . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The presence of messenger RNA for specific cytokines ( interleukins 1 through 6 , chemotactic factors , granulocyte and granulocyte macrophage colony stimulating factor ( GM CSF ) , tumor necrosis factor ( TNF alpha ) and beta , IFN gamma , transforming growth factor ( TGF beta ) , and cytokine synthesis inhibitory factor ( CSIF ) / IL 10 ) was determined by polymerase chain reaction ( PCR ) amplification of reverse transcribed RNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cell free supernatant from this A 10 line , as well as from immune spleen cells stimulated with specific antigen or concanavalin A , but not from the A 28 T cell line also activated the trypanocidal activity of peritoneal macrophages or of the J 774 macrophage like cell line . when the lymphokine content of the supernatants from both cell lines was analyzed , it was found that the A 10 T cell line secreted interferon ( IFN ) gamma , tumor necrosis factor ( TNF ) alpha and interleukin 2 , whereas the A 28 line did not secrete IFN gamma upon stimulation . ^^^ Furthermore , the trypanocidal inducing ability of A 10 supernatant was completely abrogated by neutralizing anti IFN gamma antibodies and partially abrogated by neutralizing anti TNF alpha antibodies . ^^^ When recombinant cytokines were added to J 774 cells , IFN gamma was able to induce significant trypanocidal activity whereas TNF alpha was almost ineffective . ^^^ However , TNF alpha or lipopolysaccharide ( LPS ) showed a synergistic effect with IFN gamma on macrophage activation . ^^^ IFN gamma triggered nitric oxide ( NO ) synthesis by J 774 cells whereas TNF alpha was almost ineffective . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PAM with TNF alpha pretreatment , and PAM with IFN gamma pretreatment could release increased amount of O 2 significantly , compared with control . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
While the preoperative production of tumor necrosis factor ( TNF ) and interleukin 1 alpha ( IL 1 alpha ) was elevated , that of lymphotoxin ( LT ) and interferon gamma ( IFN gamma ) were slightly suppressed . ^^^ In the postoperative period the peak TNF and IL 1 alpha and active oxygen productivity was elevated , while LT and IFN gamma productivity was suppressed in patients with an intraoperative bleeding volume of more than 1 , 000 ml compared to those with that of less than 1 , 000 ml . ^^^ Thus , stress stimulates the TNF and IL 1 alpha and active oxygen producing system , that is , the macrophage neutrophil system , and suppresses the LT and IFN gamma system , being , the inflammatory helper T cell system , in the early postoperative period . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
And serum or plasma levels of cytokines , including tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) and immunosuppressive acidic protein ( IAP ) , were also measured in patients with ATL , healthy HTLV 1 carriers and healthy HTLV 1 non carriers as controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulated cell supernatant or recombinant IFN gamma alone did not induce TNF production . ^^^ A combination of LPS with stimulated cell supernatant or IFN gamma had only a limited synergistic effect on TNF production by Am phi s . ^^^ However , both LPS and stimulated cell supernatant or recombinant IFN gamma induced maximum TNF production by RPm phi s and TGPm phi s . ^^^ TGPm phi s showed greater sensitivity to LPS and stimulated cell supernatant or IFN gamma with regard to TNF production than the other macrophage populations investigated . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Because interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) have been shown to activate macrophages to kill a variety of microorganisms , the effects of IFN gamma and TNF alpha on the activation of murine macrophages and human monocytes to kill nocardiae were studied . ^^^ It was found that macrophages or monocytes treated with either IFN gamma , TNF alpha , or lipopolysaccharide as a secondary signal did not demonstrate increased microbicidal activity against N . asteroides even though these phagocytes were effective at killing the fungus Coccidioides immitis and the protozoan Toxoplasma gondii . ^^^ Therefore , the specific timing of the exposure of the phagocyte in vitro to IFN gamma or TNF alpha has a significant effect on its ability to alter nocardial growth . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In four clinical studies chronic myelogenous leukemia ( CML ) patients were treated with interferon ( IFN ) alpha alone or IFN alpha combined with either low dose IFN gamma or tumor necrosis factor ( TNF ) alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When the clones were restimulated for 24 hr with IL 2 and immobilized antibodies to CD 3 , CD 8 , and LFA 1 , most secreted IL 3 and IFN gamma and about a third secreted TNF . ^^^ Similarly , analysis of several clones for lymphokine mRNA expression following cDNA amplification by the polymerase chain reaction revealed the presence of IL 2 , IL 3 , IFN gamma , TNF alpha , and GM CSF but not IL 4 or IL 6 transcripts . ^^^ Cytolytic CD8+ T cell clones generated in this high efficiency , accessory cell independent cloning system therefore expressed a restricted lymphokine profile characterized by the synthesis of IL 3 and IFN gamma , with variable production of TNF . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The present study examined the importance of endogenous tumor necrosis factor alpha ( TNF alpha ) and gamma interferon ( IFN gamma ) in the clearance of P . carinii by treatment of reconstituted SCID mice with anti TNF alpha and anti IFN gamma immunoglobulin G ( IgG ) . ^^^ The importance of endogenous TNF alpha in the clearance of P . carinii was further supported by the finding of TNF alpha but not IFN gamma in lung homogenate supernatants from reconstituted SCID mice . ^^^ These results strongly suggest that , in reconstituted SCID mice , endogenous TNF alpha is important in host resistance against P . carinii infection , whereas IFN gamma appears not to play a significant role . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
As is emerging in other fields , study of interleukin 1 alone , and synergistically with TNF and IFN gamma , warrants further exploration in parasitic diseases . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor ( TNF ) and interferon gamma ( IFN gamma ) are pluripotent cytokines and have multiple functions during the inflammatory response . ^^^ Using a murine model of autoimmune myocarditis , we studied the role of TNF and IFN gamma in myocardial inflammation . ^^^ Neutralizing monoclonal antibodies against TNF alpha / beta and IFN gamma were administered to myosin immunized A / J mice to assess the effect on the severity of myocardial inflammation . ^^^ Tumor necrosis factor ( TNF ) and interferon gamma ( IFN gamma ) are pluripotent cytokines and have multiple functions during the inflammatory response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression was markedly stimulated by interferon gamma ( IFN gamma ) , was varying between fibroblast lines stimulated or depressed by interleukin 1 alpha ( IL 1 alpha ) , was intermediately depressed by tumor necrosis factor alpha ( TNF alpha ) , and markedly depressed by transforming growth factor beta ( TGF beta ) . ^^^ TNF alpha , however , enhanced the stimulation by a high dose of IFN gamma , whereas TGF beta markedly depressed the stimulations given by IFN gamma and IL 1 alpha . ^^^ TNF alpha , IL 1 alpha , and IFN gamma stimulated the Mn SOD activity , as previously known , and these responses were reduced by TGF beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The LMF b primarily acted on T lymphocytes , whereas tumor necrosis factor as well as IFN gamma acted on HEV cells or fibroblast cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We detected cells expressing interleukin 1 beta ( IL 1 beta ) , tumor necrosis factor alpha ( TNF alpha ) , IL 6 , IL 2 , and interferon gamma ( IFN gamma ) genes in all lymph nodes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Detection of endogenous IFN gamma and TNF alpha in tumor infiltrating mononuclear cells of human colorectal cancer ] . ^^^ Quantitative evaluation of the levels of endogenous gamma interferon ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) in the extracts of tumor , peritumoral and normal colorectal tissues resected surgically from 43 patients with colorectal adenocarcinoma was carried out using solid phase , sandwich radioimmunoassay ( RIA ) . ^^^ The levels of both IFN gamma and TNF alpha detected in the tumor tissues were higher than those in the peritumoral and normal tissues obtained from each patient . ^^^ A significant negative correlation was observed between the levels of IFN gamma and TNF alpha in each tumor tissue . ^^^ The immunohistochemical staining of IFN gamma and TNF alpha producing cells in tumor tissues represented that IFN gamma was mainly produced by CD4+CD8 CD11c lymphocytes and that TNF alpha was mainly produced by CD 4 CD8 CD11c+ cells with macrophage like morphology . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Administration of monoclonal IFN gamma antibodies in C57BL / 10ScSn mice up to day 3 of P . acnes treatment completely inhibited the overproduction of TNF alpha by LPS . ^^^ The effect of exogenously administered IFN gamma on LPS sensitivity ( e . g . , TNF alpha production , lethal effects ) was studied in LPS responder and nonresponder mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The in vitro production of TNF alpha , IFN gamma , and IL 1 followed the same cyclic increase during treatment periods . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum samples taken before , during and following sequential courses of IL 2 were assayed for the presence of tumour necrosis factor alpha ( TNF alpha ) IL 1 alpha , IL 6 and interferon gamma ( IFN gamma ) and the presence or changes in these cytokines were examined with respect to clinical response data : our results did not show any direct correlation between the parameters measured and clinical outcome . ^^^ During one or more courses of treatment only 1 patient , who had metastatic bone disease , demonstrated detectable serum TNF alpha ; serum IL 6 levels were elevated in a proportion of all patients studied and a sustained IL 6 response occurred in a patient who had complete disease remission ; IL 1 alpha was detected in the serum of 3 RCC patients ; IFN gamma could not be detected in any serum sample tested . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moderate to high levels of hsp 60 mRNA and protein could be induced in the THP 1 monocytic leukemia cell line by heat shock , retinoic acid , interferon ( IFN ) gamma or tumor necrosis factor ( TNF ) alpha treatment , the highest levels obtained with a combination of IFN gamma / TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This paper examines TEC in sections from autoimmune thyroiditis for the in vivo production of tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) using the combined techniques of in situ hybridization and immunohistochemistry . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In HL 60 and ML 3 human myeloid cell lines , gamma interferon ( IFN gamma ) and / or tumor necrosis factor ( TNF ) induce synergistic accumulation of transcripts of the genes encoding the heavy chain ( gp 91 phox ) of cytochrome b 558 and the cytosolic factors p 47 phox and p 67 phox , components of the superoxide generating NADPH oxidase system . ^^^ The accumulation of transcripts for gp 91 phox and p 47 phox , as quantitated at the single cell level by in situ hybridization , is extremely heterogeneous ; however , when the cells are stimulated by IFN gamma and TNF together , most or all the cells in the induced cultures express higher accumulation of gp 91 phox and p 47 phox transcripts than cells from uninduced culture . ^^^ These results suggest that all the cells in the culture are induced to differentiate by TNF and IFN gamma but that at the time of analysis there is heterogeneity in the level of differentiation and a proportion of cells is present that shows more mature characteristics with a coordinate expression of the various differentiation markers and functions . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Administration of IFN gamma before LPS in HeJ mice had no effect on IL 1 mRNA , but partially restored the LPS induced increase in hepatic and splenic mRNA for TNF and serum TNF levels , which may account for the partial restoration of sensitivity to the lipogenic effect of LPS after IFN gamma treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Exposure of monocyte cultures to IFN gamma ( 100 U / mL ) either before or after ExP further enhanced TNF production by 4 to 28 times . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , no negative regulators of hematopoiesis , such as interferon gamma ( IFN gamma ) or tumor necrosis factor alpha ( TNF alpha ) , were found in plasma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effect of interferon gamma ( IFN gamma ) priming of murine peritoneal macrophages on the activation to tumouricidal state by cisplatin , lipopolysaccharide ( LPS ) IL 1 and TNF was investigated . ^^^ Cisplatin , LPS , IL 1 or TNF treated IFN gamma primed macrophages showed significantly enhanced tumouricidal activity and binding to tumour cells , compared with unprimed treated or untreated macrophages . ^^^ Macrophages treated with cisplatin , LPS , IL 1 and TNF produced released and membrane associated IL 1 and TNF activity which was significantly enhanced after priming with IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines like tumor necrosis factor ( TNF ) , interferon gamma ( IFN gamma ) , and interleukin 1 ( IL 1 ) are known to interfere with the differentiation of cultured cell lines of adipocyte precursors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Thioglycollate elicited peritoneal exudate macrophages incubated with native doublet MIP 1 secreted bioactive TNF and IL 6 , as well as immunoreactive IL 1 alpha , and these effects were enhanced significantly when the cells were costimulated with IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) enhanced TNF induced cytotoxicity of the regressor tumor cells , but it had no effect on Ha ras induced progressor tumor variants . ^^^ The resistance of the Ha ras induced progressor variants to TNF and IFN gamma could be attributed to a decrease in the number of TNF receptors on their cell surface . ^^^ However , there was no correlation between TNF and IFN gamma sensitivity of tumor cells and sensitivity to killing by activated macrophages , NK or NC cells . ^^^ These results indicate that in some murine tumor cells , there may be a relationship between in vivo tumor growth and in vitro resistance to cytolysis by TNF and IFN gamma . ^^^ Although the response of the Ha ras induced progressor variants to TNF and IFN gamma produced endogenously in immunocompetent mice is unknown , one can infer that some tumors escape the immune defenses of the normal host by becoming resistant to certain cytokines produced by the immune system . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of four myelosuppressive cytokines , including tumor necrosis factor ( TNF ) , lymphotoxin , macrophage inflammatory protein 1 alpha ( MIP 1 alpha ) , and interferon gamma ( IFN gamma ) was examined using polymerase chain reaction ( PCR ) assisted messenger RNA ( mRNA ) amplification . mRNA for TNF , lymphotoxin , and MIP 1 alpha was readily detectable at variable levels in BMMC from normal and transfused controls as well as in BMMC from aplastic patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
HIV 1 RNA and protein levels in infected Dami cells were enhanced by treatment with tumor necrosis factor alpha ( TNF alpha ) , and decreased by treatment with interferon alpha ( IFN alpha ) and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma enhanced the monocyte release of TNF alpha by 3 7 . 5 fold ( agonist dependent ) when added to patient ' s cells in vitro , and this could be suppressed by the in vitro addition of 10 micrograms / ml of thalidomide . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Antibodies of human TNF alpha , TNF beta , IFN alpha , IFN gamma , and TGF beta failed to prevent F 2 mediated cytotoxicity to K 562 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have analysed the natural immune response to leptomeningeal tumor invasion in 43 Patients by assessing cerebrospinal fluid ( CSF ) levels of albumin , IgG , IgM , interleukins ( IL ) 1 , 2 , 4 and 6 , soluble IL 2 receptor ( sIL 2R ) , interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and the tumor markers , carcinoembryonic antigen ( CEA ) and alphafetoprotein ( AFP ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The combination of interferon gamma IFN gamma and TNF alpha further enhanced the augmented IL 6 mRNA accumulation seen with TNF alpha alone ( P less than 0 . 05 ) . ^^^ Furthermore , IFN gamma and TNF alpha were found to enhance IL 6 bioactivity synergistically ( P less than 0 . 05 ) . ^^^ We conclude that IL 6 is produced by RCC ; this production is enhanced by TNF alpha with synergistic effects seen with IFN gamma at both mRNA and protein levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The proportion of cells containing mRNA able to hybridize to the antisense c DNA probes for interleukin 2 ( IL 2 ) , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) or receptors for IL 2 ( either p 55 or p 70 ) was also determined in human normal peripheral lymphoid tissues and inflammatory tissues . ^^^ In contrast , tumor infiltrating lymphocytes ( TIL ) in the stroma of ovarian carcinomas or most ductal breast tumors only rarely expressed mRNA for TNF alpha , IL 2 or IFN gamma . ^^^ In those ductal breast carcinomas which contained intracellular or intraductal mucins , up to 30 % of lymphoid cells in the tumor stroma were positive for IL 2 , TNF alpha , IFN gamma and IL 2R . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Growth restraint and differentiation by LPS / TNF alpha / IFN gamma reorganization of the microtubule network in human leukemia cell lines . ^^^ Re organization of the MT network was observed in HL 60 and U 937 AML cells treated with combined lipopolysaccharide ( LPS ) , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) . ^^^ In contrast , KG 1 cells exhibited growth restraint , but did not re organize with LPS / TNF alpha / IFN gamma treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Comparison of acutely rejecting allografts in untreated rats with well functioning allografts collected at day 7 post Tx from CD 4 treated rats showed that CD 4 mAb : ( 1 ) significantly reduced mononuclear cell infiltration , interstitial edema , hemorrhage formation and vascular and extravascular thrombosis ; ( 2 ) inhibited mononuclear cell induction of receptors for IL 2 and transferrin , and upregulation of class 2 antigens and ICAM 1 on leukocytes and endothelial cells ; ( 3 ) suppressed intragraft mononuclear cell and / or endothelial production of the cytokines IL 1 , IL 2 , IL 6 , IFN gamma , and TNF ; and ( 4 ) blocked upregulation of endothelial tissue factor and downregulation of thrombomodulin , and consequently inhibited fibrin deposition . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
T cell clones derived from cerebrospinal fluid ( CSF ) of patients with multiple sclerosis ( MS ) were analysed for their capacity to produce interleukin 2 ( IL 2 ) , interleukin 4 ( IL 4 ) , interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^ All the CSF T clones ( both CD4+ and CD8+ ) produced large amounts of IFN gamma and particularly of TNF alpha , that was synthesized in a significantly larger amount than compared clones . ^^^ From our results , we conclude that first , the CSF CD4+ T clones could constitute a subset with functional properties similar to the T helper 1 ( Th 1 ) / inflammatory cell subset of the mouse ; and second , the large amounts of TNF produced by CSF T cell clones strongly suggests a significant role for this cytokine as well as of IFN gamma in MS immunopathogenesis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Several examples have been demonstrated including IFN gamma and TNF and AZT and IFN alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Simultaneous treatment with IFN gamma and IFN beta or TNF alpha , which by itself was unable to induce the expression of class 2 HLA antigens , produced different effects on the two osteosarcoma cell lines : in Saos 2 IFN beta and TNF alpha amplified the effects obtained with IFN gamma alone ; in U 2 OS , TNF alpha increased the expression induced by IFN gamma on class 2 HLA antigens , whereas IFN beta antagonized the effects of IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inhibition of proliferation of retrovirus immortalized macrophages by LPS and IFN gamma : possible autocrine down regulation of cell growth by induction of IL 1 and TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of MHC antigens was greatly increased after in vitro treatment with interferon gamma ( IFN gamma ) , whereas interferon alpha ( IFN alpha ) and tumor necrosis factor alpha ( TNF alpha ) increased the expression of class 1 antigens , but not of class 2 antigens . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In previously published work we reported that combined treatment with interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) markedly increased the GpIb alpha mRNA level in HUVEC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CK M stimulated thioglycollate induced peritoneal macrophages to produce interleukin 1 ( IL 1 ) and tumor necrosis factor alpha ( TNF alpha ) at concentrations of 1 100 ng / ml , and it also induced IL 2 and interferon gamma ( IFN gamma ) as well as IL 6 production by splenocytes . ^^^ Maximum production of each cytokine induced by CK M was obtained at the following doses : IL 1 at 5 ng / ml , TNF alpha at 50 ng / ml , IL 2 at 1 microgram / ml , IL 6 at 500 ng / ml and IFN gamma at 750 ng / ml . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The nature of the monocyte / T cell interaction did not appear to involve gamma interferon ( IFN gamma ) , since 10 micrograms of rabbit anti IFN gamma per ml did not neutralize TNF alpha production after TSST 1 induction . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We examined the recombinant cytokines interferon ( IFN ) alpha , IFN gamma , granulocyte macrophage colony stimulating factor ( GM CSF ) , and macrophage colony stimulating factor ( M CSF ) , alone or in combination , for their ability to induce monocytes from normal donors and patients after marrow grafting to express TNF alpha mRNA and secrete TNF alpha bioactivity . ^^^ Only the combination of IFN gamma / GM CSF was consistently capable of inducing substantial TNF alpha mRNA transcript levels and protein secretion . ^^^ Levels of TNF alpha transcripts induced by IFN gamma / GM CSF were maintained for at least 36 h , in contrast to lipopolysaccharide ( LPS ) stimulation which caused TNF alpha mRNA levels to peak after 2 h and decline rapidly thereafter . ^^^ IFN gamma / GM CSF was also capable of inducing a prolonged ( at least 48 h ) secretion of TNF alpha bioactivity . ^^^ When monocytes were incubated with IFN gamma alone ( ' priming ' ) , washed and then exposed to GM CSF , both TNF alpha mRNA expression and TNF alpha protein production occurred . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast to anti IL 5 and anti TNF mAb , an anti IFN gamma mAb interfered with the anti tumor response demonstrating the involvement of IFN gamma during the IL 4 induced tumor suppression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Measurement of concentrations of TNF , granulocyte macrophage CSF , IL 6 , IL 10 , IFN gamma , IL 2 , and IL 4 in cell culture supernatants indicated that cytokine release correlated with mRNA patterns . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both IL 4 and IL 10 [ cytokine synthesis inhibitory factor ( CSIF ) ] inhibit IL 2 induced synthesis of IFN gamma and tumor necrosis factor ( TNF ) alpha by human PBMC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The role of TNF alpha and IFN gamma in various models of autoimmune disease were analyzed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Production of macrophage colony stimulating factor ( M CSF ) by human monocytes is differentially regulated by GM CSF , TNF alpha , and IFN gamma . ^^^ Both TNF alpha and IFN gamma enhanced M CSF message levels induced by GM CSF , but only TNF alpha synergized with GM CSF in the induction of M CSF protein secretion . ^^^ M CSF transcripts induced by TNF alpha and IFN gamma were much lower compared to those induced by GM CSF and PMA and were not accompanied by the secretion of M CSF protein . ^^^ In addition , costimulation of cells with TNF alpha and IFN gamma did not result in M CSF production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumour necrosis factor ( TNF ) and interferon gamma ( IFN gamma ) induce differentiation of human myeloid cell lines along the monocytic lineage . ^^^ In this study we investigated the effects of TNF and IFN gamma on the expression of the CD 4 protein and messenger RNA ( mRNA ) in the two myeloid cell lines , ML 3 and HL 60 . ^^^ We observed that CD 4 antigen expression on ML 3 cells is almost undetectable and that TNF and IFN gamma induced CD 4 antigen expression on these cells . ^^^ HL 60 cells express surface CD 4 antigen at high density and treatment with TNF and IFN gamma caused a decrease of CD 4 expression . ^^^ TNF induced CD 4 mRNA in ML 3 cells and IFN gamma synergistically potentiated the effect of TNF , thus indicating that the enhanced expression of the CD 4 protein on ML 3 cells is due , at least in part , to an enhanced accumulation of the CD 4 mRNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It has been demonstrated that activation of mouse brain endothelium by TNF alpha and IFN gamma led to accumulation of nitrite which is presumably formed by oxidation of nitric oxide . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lymphocytes , collected from efferent prefemoral lymph , labelled in vitro with [ 111In ] oxine and reinjected intravenously , migrated in large numbers into delayed type hypersensitivity ( DTH ) reactions elicited by purified protein derivative ( PPD ) and into sites stimulated with tumour necrosis factor alpha ( TNF alpha ) or interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serial blood samples were collected after L MTP PE administration and were assayed for cytokine levels ( tumor necrosis factor alpha [ TNF alpha ] interleukin 1 alpha [ IL 1 alpha ] , IL 1 beta , IL 6 , interferon gamma [ IFN gamma ] , neopterin , C reactive protein ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , macrophages stimulated in teflon containers by IFN gamma showed a strong PCA response peaking around 24 hr after stimulation , but they failed to secrete tumour necrosis factor ( TNF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In particular , interleukin 1 ( IL 1 ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) , acting synergistically at low , subthreshold concentrations , are able to completely inhibit the growth of endothelial cells in vitro . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Resident macrophages treated with interferon gamma ( IFN gamma ) , lipopolysaccharide ( LPS ) plus muramyldipeptide ( MDP ) expressed a cytostatic activity against P 815 tumor cells and released interleukin 6 ( IL 6 ) and nitrite but produced neither IL 1 nor tumor necrosis factor ( TNF ) . ^^^ Thioglycollate elicited macrophages required only LPS plus IFN gamma for cytostatic activity which was expressed concomitantly with the release of high levels of TNF , IL 1 and IL 6 , whereas C3H / HeJ macrophages produced low levels of monokines and were not cytostatic . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Amoebic liver abscess derived macrophages pretreated with either recombinant gamma interferon ( IFN gamma ) or the cyclooxygenase inhibitor indomethacin augmented TNF production in response to soluble amoebic proteins and LPS . ^^^ Macrophages from infected and naive animals pretreated with recombinant IFN gamma or indomethacin produced increased amounts of TNF in response to LPS but not in response to soluble amoebic protein stimulation . ^^^ In contrast , IFN gamma and LPS activated naive macrophages produced enhanced levels of TNF in response to live amoebae and soluble amoebic proteins . ^^^ Our results demonstrate that TNF production by macrophages is altered during E . histolytica infection and in response to amoebae and suggest a role for IFN gamma and prostaglandin E 2 in regulating TNF production during the infection . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Animals were monitored for differences in behavior , histopathologic studies , survival , and serum levels of tumor necrosis factor ( TNF alpha ) , interferon gamma ( IFN gamma ) , and interleukin 6 ( IL 6 ) after lipopolysaccharide . ^^^ Northern blot analyses of TNF , IFN gamma , c fos , and IL 6 transcripts were also performed . ^^^ TNF levels were significantly lower in the PBN treated animals at 1 to 6 hours , whereas IFN gamma levels were depressed at 8 hours . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We examined the responsiveness of two factor dependent macrophage cell lines , BDM 1 and its subclone , BDM 1W3 , to bacterial lipopolysaccharide ( LPS ) , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) for their growth . ^^^ In contrast to LPS and IFN gamma , TNF alpha did not inhibit the proliferation of BDM 1 and BDM 1W3 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Monocytes primed with GM CSF or IFN gamma also maintained LPS responses for production of tumor necrosis factor alpha ( TNF alpha ) and interleukin 1 beta ( IL 1 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It could be completely eliminated by cotreatment of cells with anti IL 6 antibodies , but not by treatment with anti interferon gamma ( IFN gamma ) , suggesting that IFN gamma , which can enhance TNF receptor expression on a variety of cells , was not a mediator in this IL 6 effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines secreted by TIL cells in vitro ( IL 2 , IL 4 , IFN gamma , TNF alpha ) may be involved in their antitumorigenic activity . ^^^ Moreover , these data implicate that CD 4 positive TIL derived from CTCL can not be grouped into different subsets based on the production of IL 2 , IL 4 , IFN gamma , and TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Other inflammatory mediators such as IFN gamma , fibroblast growth factor , and TNF alpha fail to provoke the production of . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Anti IFN gamma also significantly decreased PM phi O 2 and TNF release . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We now report that stimulation of macrophages either with recombinant human gamma interferon ( IFN gamma , 10 ( 2 ) U / ml ) or with recombinant human TNF alpha ( 10 ( 2 ) U / ml ) resulted in an increase in the intracellular concentration of azithromycin by approximately 200 % within 3 h , compared with the concentration in unstimulated macrophages . ^^^ Stimulation of infected macrophages with recombinant IFN gamma or TNF alpha overcame the inhibitory effect associated with infection . ^^^ These results suggest that the increased bactericidal activity of the TNF alpha azithromycin or IFN gamma azithromycin combination against M . avium is related to enhanced uptake of the antibiotic by the stimulated phagocyte . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines in the pathogenesis of malaria : levels of IL 1 beta , IL 4 , IL 6 , TNF alpha and IFN gamma in plasma of healthy individuals and malaria patients in a holoendemic area . ^^^ Plasma concentration of TNF alpha and IFN gamma were higher in parasitaemic than aparasitaemic individuals and donors who had clinical malaria had higher levels of TNF alpha , IFN gamma and IL 6 than asymptomatic parasitaemic donors . ^^^ There was a negative correlation between age of the individual and the concentration of plasma TNF alpha and IFN gamma suggesting that the production of these cytokines could be modulated by repeated malarial infections . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The enhancement of the cytotoxic effect of TNF by IL 4 against various tumor cell lines was found comparable with that by gamma interferon ( IFN gamma ) . ^^^ Pre exposure of U 937 cells to IFN gamma led to a greater than 2 fold induction in TNF receptors , but no modulation of TNF receptors by IL 4 was observed . ^^^ Moreover , the presence of IFN gamma was found to further potentiate the antiproliferative effects of TNF and IL 4 . ^^^ These results clearly suggest that IL 4 potentiates the antiproliferative responses of TNF by a mechanism different from that of IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A number of recombinant plasmids coding for fusion proteins between human interferon gamma ( IFN gamma ) and human tumour necrosis factor alpha ( TNF alpha ) or beta ( TNF beta ) were constructed by using site directed mutagenesis and ligation of the respective genes . ^^^ In these proteins the whole IFN gamma sequence of the molecule is linked at the N terminus via a short polypeptide linker to the TNF alpha sequence lacking two N terminal amino acid residues or to the whole TNF beta sequence . ^^^ Bacterial cell lysates expressing these fusion genes or their deletion mutants showed both biological activities in vitro : the antiviral activity of IFN gamma and the cytotoxic activity of TNF . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The lymphokines IFN gamma and TGF beta 2 both decreased the AMLR in MS patients and controls while TNF had no effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma or TNF alpha also induced ICAM 1 on KC , but these KC failed to stimulate proliferation , suggesting that PMA induces additional signals from KC , which act in concert with ICAM 1 to promote proliferation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Depletion of these cells or addition of anti IFN gamma antibodies abolished IFN production and reduced TNF secretion by adherent cells to the levels found in the cultures of mannoprotein stimulated spleen cells from naive mice . ^^^ These data add further evidence to the immunomodulatory properties possessed by some cell wall constituents of the human commensal microorganism C . albicans and suggest that IFN gamma is endowed with a regulatory role in TNF production by mouse macrophages in vitro . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After 48 hours ' exposure to immune mediators , such as culture supernatant of phytohemagglutinin ( PHA ) stimulated lymphocytes , interferon gamma ( IFN gamma ; 1 , 000 U / ml ) , tumor necrosis factor alpha ( TNF alpha ; 2 , 000 U / ml ) , interleukin 1 alpha ( IL 1 alpha ; 1 , 000 U / ml ) and lipopolysaccharide ( LPS ; 50 micrograms / ml ) , ICAM 1 expression on both cell types was markedly increased in terms of intensity and cell numbers . ^^^ IFN gamma and culture supernatant of PHA stimulated lymphocytes were the most potent inducers of ICAM 1 among the mediators tested , while TNF alpha , IL alpha and LPS were less effective , although variations were observed among cultures derived from different donors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both IFN gamma and TNF induced large increases in the ICAM 1 expression on both cell lines and increased the susceptibility of the tumor cells to monocyte mediated killing . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Large amounts of neopterin are released by human macrophages on stimulation with interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) further enhances the effect of IFN gamma . ^^^ Therefore , our data suggest that activated immune cells and specific cytokines such as IFN gamma and TNF alpha are involved inhibiting hematopoiesis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
One clone is of the Th 2 type as it produces IL 4 , IL 5 , and large amounts of IL 6 , but not IL 2 , IFN gamma or TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Among several recombinant human cytokines tested , i . e . , interferon gamma , tumor necrosis factor alpha , interleukin 1 alpha and beta , and IL 4 , IFN gamma , TNF alpha , and IL 1 alpha / beta were shown to augment the expression of ICAM 1 . ^^^ MLR supernatants and IFN gamma were more effective in augmenting ICAM expression than TNF alpha and IL 1 alpha / beta . ^^^ The MLR supernatants were shown to contain IFN gamma and TNF alpha , and the activity of the MLR supernatant was partially inhibited by neutralizing antibody against IFN gamma . ^^^ These data suggest that cytokines , especially IFN gamma , TNF alpha , and IL 1 alpha / beta , released by T cells and antigen presenting cells upon recognition of alloantigens upregulate ICAM 1 expression on renal tubular epithelial cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Flow cytometric analysis showed that HLA expression in this cell line is readily induced by IFN gamma and modulated by TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It is induced by proinflammatory cytokines such as IL 1 , TNF alpha , or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The TLC from the atopic donors closely resembled murine type 2 Th ( Th 2 ) cells by secreting substantial IL 4 , IL 5 , IL 6 , TNF alpha , and granulocyte macrophage ( GM ) CSF , minimal IFN gamma , and relatively little IL 2 . ^^^ In contrast , the nonatopic ' s TLC resembled murine type 1 Th ( TH 1 ) cells by secreting substantial IFN gamma , IL 2 , TNF alpha , and GM CSF , no IL 4 , and little IL 5 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , the well known synergistic effect of IFN gamma plus TNF alpha on keratinocyte ICAM 1 induction could be mimicked by stimulation of cells with IFN gamma plus anti 55 kd TNFR antibodies . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In keeping with these biological activities and protein data , Northern blot analysis of total cellular RNA extracted from keratinocyte monolayers hybridized with a 32P labelled 1 kb cDNA to IL 8 mRNA , revealed induction of the IL 8 gene in the presence of TNF alpha and IL 1 beta , but not IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These studies revealed that sorted DN T cells did not secrete IL 3 , IL 4 , IL 5 , IL 6 , GM CSF , TNF alpha , or IFN gamma spontaneously or after TCR alpha / beta cross linking . ^^^ In contrast , stimulated unfractionated lpr and gld LN cells proliferated strongly and secreted high levels of IFN gamma and TNF alpha and low levels of IL 3 , IL 4 , and IL 6 . ^^^ In contrast to normal CD4+ T cells , lpr and gld CD4+ Ly 5 ( B 220 ) + T cells proliferated weakly and did not secrete TNF alpha , IL 2 , or , in most experiments , IFN gamma after stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These results suggest that cytokines which could be produced by tumor infiltrating mononuclear cells , especially IFN gamma and TNF alpha , might modulate expression of MHC antigens and ICAM 1 , and influence host immune response against RCC . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Shedding of ICAM 1 could be induced by IFN gamma and TNF alpha alone , or more effectively , by a combination of the two cytokines together . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This suppressive influence of the supernatant was almost completely neutralized by the addition of a combination of anti human tumor necrosis factor alpha ( HuTNF alpha ) and anti human interferon gamma ( HuIFN gamma ) antibodies , and it has been speculated that this suppressive influence of the supernatant is probably due to TNF alpha and IFN gamma . ^^^ The numbers of M and GM progenitors increased remarkably in the assays in which the supernatant was added when compared to assays without the supernatant ; this suggests that these progenitors were resistant to TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma and TNF increased antigen expression ( 30 50 % ) which lasted for 4 6 days after treatment . ^^^ When cells were incubated with IFN gamma or TNF for 2 days prior to hyperthermia , the increase in antigen expression was observed immediately after heating , but by the following day , antigen expression was similar to that after heat treatment alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Complex effects of principal inflammatory cytokines ( IL 6 , IL 1 , TNF , IFN gamma ) on acute phase protein synthesis and other metabolic processes in cultured liver cells are briefly reviewed . 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma and TNF alpha were essential to the killing mechanism whereas Il 1 , Il 2 , and Il 4 were not required . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Low amounts of IFN gamma , TNF alpha , and IL 1 beta were detected in the supernatants and extracts of 6 wk FL cells . ^^^ In conclusion , the expression of HLA class 1 and 2 Ag is not detected on primitive megaloblasts , but is gradually induced on definitive FL progenitors and precursors , possibly via production of specific cytokines in the FL microenvironment , i . e . , IFN gamma and TNF alpha for class 1 Ag and IL 1 beta for class 2 Ag . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The ability of EC to affect levels of IL 2 can not be reproduced by soluble factors , including the cytokines IL 1 , IL 6 , IFN gamma , or TNF , conditioned medium from resting EC or IL 1 , IFN gamma or TNF treated EC , or from resting PBMC + EC cultures . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We studied the adhesion of human peripheral blood T lymphocytes to human synovial fibroblasts stimulated with interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 beta ( IL 1 beta ) , or combinations of these cytokines . ^^^ IFN gamma treatment resulted in the largest increase in adhesion , followed by TNF alpha and IL 1 beta . ^^^ Combinations of IFN gamma + TNF alpha and IFN gamma + IL 1 beta had a synergistic effect on intercellular adhesion molecule 1 ( ICAM 1 ) expression and adhesion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It was also enhanced by exogenous IL 1 and IL 6 , but not by IL 3 , tumour necrosis factor alpha ( TNF alpha ) or interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin 6 secretion by PMN , however , required previous stimulation with GM CSF or tumor necrosis factor alpha ( TNF alpha ) , whereas other cytokines , including interleukin 3 ( IL 3 ) , granulocyte CSF ( G CSF ) , macrophage CSF ( M CSF ) , interferon gamma ( IFN gamma ) , and lymphotoxin ( LT ) , failed to induce IL 6 mRNA accumulation and protein secretion by PMN . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cells from approximately half of the AIDS patients , in contrast to none from the control group , showed histamine release after stimulation with interleukin 4 ( IL 4 ) , tumor necrosis factor alpha ( TNF alpha ) , lymphotoxin ( LT ) and interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , IL 4 exhibited significant synergy with IL 1 or TNF in inducing 1 . 4C3 Ag expression ( p less than 0 . 001 ) but inhibited the increased expression of ICAM 1 produced by IL 1 , TNF , or IFN gamma ( p less than 0 . 01 ) and inhibited the induction of ELAM 1 by IL 1 and TNF ( p less than 0 . 001 ) . ^^^ Finally , although IL 4 alone caused little if any shape change in EC monolayers , it strongly synergized with TNF or IFN gamma in causing a change in shape to a more fibroblastic morphology . ^^^ Furthermore , the ability of IL 4 to both enhance and inhibit the expression of activation Ag on EC already activated by IL 1 , TNF , or IFN gamma suggests that it may be important in altering the quality of inflammatory responses such as may occur during the development and maintenance of chronic or immune mediated inflammation . . ^^^ IL 4 regulates endothelial cell activation by IL 1 , tumor necrosis factor , or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both TNF and LT mRNA were minimally induced by IL 1 alpha , IL 3 , interferon ( IFN ) alpha , or IFN gamma . ^^^ In conjunction with IL 2 , cytokines such as IFN alpha , IFN gamma , or TNF did not interfere with IL 2 induction of TNF and LT mRNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression and occupation of urokinase type plasminogen activator receptors ( u PA R ) are known to be up regulated by IFN gamma and TNF alpha , and endogenously occupied u PA R were found to be instrumental in monocyte invasiveness . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study shows that , even though the absolute number of T lymphocytes and helper cells are reduced in the long term observation after DCF treatment , the capacity to produce IL 2 , TNF , and IFN gamma was within normal range . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma , which by itself increases EC class 1 MHC and ICAM 1 but does not induce ELAM 1 expression , has been found to act synergistically with TNF to increase class 1 expression . ^^^ In our study , we have further examined IFN gamma effects on both TNF and IL 1 beta responses . ^^^ In contrast to IFN gamma plus TNF cotreatment , IFN gamma up regulation of class 1 MHC molecules is not augmented by cotreatment with IL 1 beta . ^^^ IFN gamma plus TNF cotreatment synergistically increases ICAM 1 expression by 24 h of cotreatment , whereas IFN gamma plus IL 1 beta cotreated EC show at most additive increases . ^^^ IFN gamma increases TNF induced ELAM 1 expression such that a greater number of EC express ELAM 1 at both 4 and 24 h in the presence of IFN gamma plus TNF compared to cultures treated with TNF alone , although the maximal level of surface expression on individual cells is not increased . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Prestimulation as well as coincubation of IFN gamma with TNF , IL 1 , or LPS resulted in a strongly augmented production of IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Data presented here and elsewhere suggest that this CD5+ B cell inducing activity is not mediated by IL 1 , IL 2 , IL 3 , IL 4 , IL 5 , IL 6 , IFN gamma , GM CSF , or TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Coincubation of interferon gamma ( IFN gamma ) together with C . p . led to an enhanced release of TNF alpha , supporting the assumption that C . p . is a potent TNF alpha inducer . ^^^ The additive effect of IFN gamma and TNF alpha on the receptor level was demonstrated by addition of IFN gamma antibodies to the PBMC cultures . ^^^ Under these conditions TNF alpha production , stimulated by C . p . and IFN gamma , was decreased by 30 % , compared to the production in assays supplemented with C . p . alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Studies done largely with human umbilical vein EC have implicated IL 1 , IFN gamma , and TNF alpha as cytokines which promote leukocyte adhesion to EC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This activation induces a massive cytokine release in the circulation ( TNF , IFN gamma , IL 2 , IL 6 , and IL 3 ) . ^^^ At the 90 min blood withdrawal time point cytokine serum levels showed a 100 % decrease for IFN gamma , an 88 % decrease for IL 6 , and 85 % decrease for IL 2 , and a 75 % decrease for TNF . ^^^ At 4 hr IL 2 serum levels were diminished by 65 % ; IL 6 , IL 3 , and IFN gamma serum levels were comparable to controls ; and , interestingly , TNF was still detected , whereas it has already disappeared when 145 2C11 was administered alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The TNF receptor gene expression was not affected by phorbol esters , dibutyryl cAMP ( dbcAMP ) , interleukin 1 ( IL 1 ) , interferon gamma ( IFN gamma ) or by TNF , agents known to modulate functional TNF receptors . ^^^ The results suggest that the effects of phorbol diesters , IL 1 , IFN gamma , TNF and dbcAMP previously observed on binding of TNF to cells are limited to posttranscriptional regulation of the 55 kD TNF receptor . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found that interleukin 1 beta ( IL 1 beta ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) each increased the cell surface expression of intercellular adhesion molecule 1 ( ICAM 1 ) on human synovial fibroblasts in a dose and time dependent manner . ^^^ Maximal ICAM 1 expression occurred within 8 hours of induction , with the following order of efficacy : IFN gamma greater than TNF alpha greater than IL 1 beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1 , TNF , IFN gamma , and IL 4 each increase EC adhesiveness for T cells when used alone in adhesion assays in vitro . ^^^ Acting alone IL 1 , TNF , IFN gamma , and IL 4 each significantly enhanced EC adhesiveness for T cells ( p less than 0 . 005 ) , whereas only IL 1 ( p less than 0 . 005 ) and TNF ( p less than 0 . 005 ) but not IFN gamma or IL 4 significantly enhanced adhesiveness for PMN . ^^^ When EC were stimulated with optimal concentrations of TNF in combination with IL 4 or IFN gamma , there was a significant further increase in adhesiveness for T cells ( p less than 0 . 003 ) , but not PMN , over that seen with TNF alone . ^^^ The additive effect of TNF and IL 4 was more marked than that of TNF and IFN gamma . ^^^ A similar interaction with IL 4 or IFN gamma was exhibited by lymphotoxin . mAb inhibition studies indicated that the extra increase in binding caused by stimulating EC with TNF and IL 4 in combination was mediated by VCAM 1 whereas that caused by stimulating with TNF and IFN gamma in combination was substantially mediated through leukocyte function associated Ag 1 and VCAM 1 independent mechanisms . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vivo regulation of cytokine expression : effects of cycloheximide and cyclosporine ( CyA ) on IFN gamma and TNF alpha expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , unlike appropriate reference cytokines ( IL 1 and tumor necrosis factor , IFN gamma ) , G CSF and GM CSF did not modulate endothelial cell functions related to hemostasis thrombosis ( production of procoagulant activity and of platelet activating factor ) , inflammation ( expression of leukocyte adhesion molecule 1 and production of platelet activating factor ) , and accessory function ( expression of class 2 antigens of MHC ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
All monocytes exposed to lipopolysaccharide ( LPS ) , interferon gamma ( IFN gamma ) and endotoxin , PGE 2 , transforming growth factor beta 1 , whole plasma alpha globulins , purified AT and IL 6 responded equally with respect to the secretion of TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Actions of TNF and IFN gamma on angiogenesis in vitro . ^^^ TNF alpha , beta , and IFN gamma not only significantly inhibited capillary growth induced by Mf , but also blocked capillary development induced by fibroblast growth factors ( FGF ) , well known potent angiogenic factors . ^^^ These actions of TNF or IFN gamma could limit vascular formation in solid tumors . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A human colon carcinoma cell subline resistant to doxorubicin ( LoVo / Dx ) , previously shown to be more lysed than the chemosensitive subline LoVo / H by different immune effectors , is reported here to be similarly susceptible to direct , anti proliferative effect of soluble cytokines ( TNF alpha and / or IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Upon stimulation with lectin and phorbol ester TcR gamma delta + lymphocytes expressed the same set of lymphokine genes as the TcR alpha beta + lymphocytes expressed the same set of lymphokine genes as the TcR alpha beta + lymphocytes , which included IL 2 , 3 , 4 , 5 , GM CSF , TNF alpha and beta , IFN gamma . ^^^ In contrast , a more limited set of lymphokine genes ( GM CSF , TNF alpha and beta , IFN gamma ) was induced in activated CD 3 NK cells , thus indicating that this subpopulation of cells may display different regulatory functions , with respect to CD3+ T lymphocytes . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Those derived from Graves ' disease expressed these molecules after stimulation with recombinant human interferon gamma ( IFN gamma ) or with recombinant human tumour necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of IL 4 , TNF , IFN gamma , and IFN alpha / beta on the macrophages ' capacity for both forms of ADCC were dose dependent . ^^^ IFN gamma and IFN alpha / beta increased the macrophages ' capacity for both variants of ADCC within 4 hr of treatment , whereas IL 4 and TNF did so only after prolonged treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this paper we show that tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) alter the expression of extracellular matrix receptors ( integrins ) in cultured human endothelial cells . ^^^ After treatment for 72 h with a combination of TNF alpha and IFN gamma , the level of the vitronectin receptor ( alpha 5 beta 3 ) at the cell surface decreases by 70 % , whereas the amounts of the beta 1 integrins remain unchanged . ^^^ The decreased expression of the alpha 5 beta 3 complex at the cell surface is due to a selective effect of TNF alpha and IFN gamma on the regulation of the beta 3 subunit synthesis at the translational level . ^^^ These data show that two important inflammatory mediators , TNF alpha and IFN gamma , can modify the interaction of endothelial cells with the extracellular matrix by selectively altering the expression of specific cell surface integrin complexes . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using RIA and bioassays , the cells were found to secrete IL 1 alpha , IL 6 , and TNF into the culture medium whereas no IL 2 , IL 4 , BCGF , CSF , or IFN gamma were detected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of THP 1 cells with IFN gamma , TNF alpha , PMA , or vitamin D 3 had no effect on Fc gamma RII expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Various factors ( IL 1 , TNF alpha , IFN gamma etc . ) have been shown to increase the endothelial adhesiveness for human lymphocytes , including natural killer cells ( NK cells ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
All clones expressed CD 56 surface antigen , displayed a strong cytolytic activity against NK sensitive ( K 562 ) and NK resistant ( M 14 ) target cells , and produced IFN gamma and tumor necrosis factor , but not IL 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Regulation of MHC molecules on MBP positive oligodendrocytes in mice by IFN gamma and TNF alpha . ^^^ A combination of IFN gamma and TNF alpha also failed to facilitate the expression of class 2 antigens on oligodendrocytes , nor did it amplify the expression of class 1 seen with IFN gamma alone . ^^^ Thus it appears that MBP+ murine oligodendrocytes are refractory to class 2 induction , and express class 1 in response to IFN gamma but not TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Production of O 2 in response to FMLP , TNF , IFN gamma , platelet activating factor , LPS , substance P , and PMA by human eosinophils in suspension and in contact with polystyrene ELISA plastic ( PL ) or biologic surfaces was studied . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of IFN gamma , TNF alpha and IL 4 were also examined . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The ability of the HIV infected TCC to produce IL 2 , IL 4 and IFN gamma in response to phorbol myristate acetate plus anti CD 3 monoclonal antibody was decreased , whereas their ability to produce TNF alpha was unaffected or even enhanced . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Within 1 hour of exposure , IFN gamma induced a twofold to fourfold accumulation of tumor necrosis factor alpha ( TNF alpha ) specific transcripts in AML blasts and several AML cell lines that include HL 60 and OCI AML 1 . ^^^ Further , the synergy between IFN gamma and IL 3 on AML blasts was partially or completely abrogated by a TNF alpha neutralizing antibody , suggesting that growth enhancement by IFN gamma may be mediated through TNF alpha production in AML blast culture . ^^^ Further , TNF alpha appears to mediate the biologic effect of IFN gamma either in growth stimulation or growth inhibition . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To investigate regulatory mechanisms of granuloma formation , we examined the modulatory effects of various mediators such as IL 1 , TNF alpha , interferon gamma ( IFN gamma ) , IL 4 , IL 6 , transforming growth factor beta ( TGF beta ) , dexamethasone and prostaglandin E 2 ( PGE 2 ) on the development of lesions , because these mediators are known to play a pivotal role in inflammatory responses . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When isolated , Leu 7 ( CD 57 ) + cells were stimulated in vitro with a mitogen that can induce peripheral blood T cells with the helper inducer phenotype to produce various cytokines , Leu 7 ( CD 57 ) + cells did not produce IL 2 , interleukin 4 ( IL 4 ) , interferon gamma ( IFN gamma ) or tumor necrosis factor alpha ( TNF alpha ) in significant amounts . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Small peritoneal exudate lymphocytes ( sPEL ) preferentially migrate from the blood to cutaneous delayed type hypersensitivity reactions and to sites injected with IFN gamma , IFN alpha / beta , and TNF alpha , rather than to peripheral lymph nodes . ^^^ VLA 4 mediates the adhesion of rat lymphocytes to rat microvascular EC stimulated with IFN gamma , mIL 1 alpha , TNF alpha , and LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Ly 6E induction mediated by IFN gamma or IFN alpha / beta was also enhanced by cotreatment with TNF alpha or IL 1 alpha , which by themselves had no detectable Ly 6E inducing effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , IFN gamma and TNF alpha synergized to increase further the expression of NO synthase . ^^^ The induction of the enzyme can be further stimulated ex vivo with IFN gamma and TNF alpha and inhibited by dexamethasone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of the LAM 1 ligand by endothelium was optimally induced by LPS and the proinflammatory cytokines TNF alpha and IL 1 beta , whereas IFN gamma and IL 4 induced lower levels of expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , the addition of the following cytokines to retinoids potentiated the retinoid induced differentiation : IFN alpha , IFN beta , IFN gamma , TNF alpha , G CSF , IL 1 alpha and IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Preincubation of cultured AuTu cells with tumor necrosis factor alpha or gamma interferon ( IFN gamma ) , but not with IFN alpha , increased their susceptibility to lysis by the T cell clones ; however , it increased resistance of AuTu to lysis by interleukin 2 activated natural killer cells . ^^^ The expression of an adhesion molecule , intercellular adhesion molecule 1 , on the surface of AuTu was also up regulated by tumor necrosis factor alpha or IFN gamma , but not by IFN alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We now report that although stimulation ( 4 48 h ) of HUVEC with various cytokines , including TNF , IL 1 , and IFN gamma , did not alter DAF levels , wheat germ agglutinin ( WGA ) ( 5 50 micrograms / ml ) , a lectin specific for binding N acetyl neuraminic acid and N acetyl glucosamine residues , increased DAF levels fivefold when incubated with HUVEC for 12 to 24 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Quantitative evaluation of the levels of interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) in the extracts of tumors and their corresponding normal tissues resected from 43 patients with colorectal adenocarcinoma was done using solid phase , sandwich radioimmunoassay . ^^^ The levels of both IFN gamma and TNF alpha detected in the tumor tissues were higher than those in the corresponding normal colorectal tissues obtained from each patient . ^^^ A significant negative correlation was observed between the level of IFN gamma and TNF alpha in each tumor extract . ^^^ Immunohistochemical staining of IFN gamma and TNF alpha producing cells in tumor tissues showed that IFN gamma was mainly produced by CD4+ CD 8 T lymphocytes and TNF alpha was mainly produced by CD11c+ cells with macrophage like morphology . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
SIL secreted significant amounts of IL 4 , GM CSF and TNF alpha upon stimulation with mitogens but failed to secrete IFN gamma . ^^^ Io in combination with phorbol ester induced the secretion of larger amounts of IL 4 , GM CSF , TNF alpha and low amounts of IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
High levels of tumour necrosis factor ( TNF ) , interferon gamma ( IFN gamma ) , IL 2 , IL 3 and IL 6 are released into the circulation 1 to 8 h after a single 10 micrograms 145 2C11 i . v . injection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To investigate the influence of inflammatory cytokines on the potential of peripheral nerves to regenerate , we analyzed the effect of interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) on the ability of immortalized Schwann cells to mediate outgrowth of neurites from primary DRG neurons . ^^^ We found that IFN gamma and TNF alpha synergistically inhibited the neurite outgrowth promoting properties of the Schwann cells by specifically down regulating myelin associated glycoprotein ( MAG ) at the levels of mRNA and cell surface protein by approximately 60 % . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Two to five days after subcutaneous immunization with antigen , lymphocytes that adhered to unstimulated and IFN gamma , TNF alpha , IL 1 alpha , and LPS treated EC were increased in the regional lymph nodes . ^^^ Lymphoblast adhesion required the stimulation of the EC with 10 times the concentrations of IFN gamma and TNF alpha required for peritoneal exudate lymphocyte adhesion . ^^^ There was a synergistic increase in the adhesion of the low density lymphocytes to EC stimulated with combinations of IFN gamma and TNF alpha . ^^^ Antibody to VLA 4 inhibited about 40 % of the stimulated adhesion to EC treated with IFN gamma , TNF alpha , or LPS . ^^^ In vivo anti VLA 4 inhibited lymphoblast migration to IFN gamma , TNF alpha , LPS , and DTH reactions by 60 % . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Levels of ICAM 1 and LFA 3 expression in normal human islet cells and regulation of their expression by cytokines interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 beta ( IL 1 beta ) , and IL 6 have been studied by two color immunofluorescence staining of pancreatic cryostat sections and fluorescence activated cell sorter analysis . ^^^ IFN gamma and TNF alpha alone or in combination strongly enhanced this spontaneous expression of ICAM 1 in a time and / or dose dependent and additive manner but had no effect on LFA 3 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After pretreatment of U 937 cells with IFN gamma to augment the sensitivity to TNF , an inhibitory effect of rADF was also found . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the case of TNF alpha , IL 6 , and G CSF , pretreatment with IFN gamma not only prevented the downregulation , but enhanced the production of these cytokines to levels higher than those obtained after the first LPS challenge . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The role ( s ) of gamma interferon ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and interleukin 4 ( IL 4 ) in establishment and maintenance of protective immunity to Francisella tularensis LVS in mice ( C3H / HeN ) was examined by selective removal of these cytokines in vivo with neutralizing antibodies . ^^^ The 50 % lethal dose ( LD 50 ) for mice infected intradermally with F . tularensis alone was 136 , 000 CFU ; treatment of mice with anti IFN gamma or anti TNF alpha at the time of infection significantly reduced ( P much less than 0 . 05 ) the LD 50 to 2 and 5 CFU , respectively . ^^^ Abrogation of protective immunity , however , was effective only when anti IFN gamma or anti TNF alpha was administered prior to day 3 postinfection . ^^^ Thus , IL 4 may be detrimental , while IFN gamma and TNF alpha were clearly crucial to the establishment of protective immunity to F . tularensis during a primary infection . ^^^ The importance of IFN gamma and TNF alpha during a secondary immune response to F . tularensis was also investigated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When HEC are exposed to a combination of tumor necrosis factor alpha ( TNF alpha ) and immune interferon ( IFN gamma ) the amount of the alpha V / beta 3 vitronectin receptor at the cell surface was decreased by a factor of 50 70 % , while the beta 1 integrin complexes were not affected . ^^^ HEC cells thus express receptors for several matrix components and inflammatory mediators such as TNF alpha and IFN gamma can selectively alter the expression of some of them . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cultivation of the cells in the presence of the macrophage activating cytokines interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) , prevented the decrease in the expression of the 60 62 kDa myristoylated protein . ^^^ The effect of cytokines was observed when monocytes were treated with IFN gamma or TNF alpha for 24 or 48 h and protein myristoylation analyzed at day four of culture . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumour necrosis factor ( TNF ) , interleukin 1 and 6 ( IL 1 and IL 6 ) and colony stimulating factor 1 ( CSF 1 ) are secreted mainly by activated macrophages , whereas T cells secrete IL 2 , IL 3 , IL 4 and interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The mRNA for cytokines IL 1 beta , IL 2 , IL 5 , IL 6 , IFN gamma , and TNF alpha are detected after T . cruzi coculture with PBMC , peaking on day 3 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The maximum tolerated dose level ( MTDL ) of 50 micrograms / m2 of TNF and 100 micrograms / m2 of IFN gamma was less than the maximum tolerated dose ( MTD ) observed in previous Phase 1 trials of gamma IFN and TNF alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The growth inhibitory response to cytokines involved in the activation and differentiation of monocytes , IFN gamma , TNF alpha , IL 1 beta , IL 6 and GM CSF , was normal . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Other cytokines ( IL 1 , IL 3 , IL 4 , IL 5 , TNF , LT , IFN gamma , and TGF beta ) had no effect on IL 2 binding , suggesting that IL 6 may mediate the enhanced expression of HIL 2 R . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Administration of anti tumor necrosis factor alpha ( TNF alpha ) antibodies to mice infected with a sublethal dose of S . typhimurium induced the same effect as anti IFN gamma antibodies , while the administration of both antibodies resulted in a synergistic interaction . ^^^ When mice were infected with an avirulent strain of S . typhimurium and challenged on day 7 either with a virulent strain of S . typhimurium or with Listeria monocytogenes , their resistance to reinfection was slightly depressed by anti IFN gamma or anti TNF alpha antibodies given 1 day before challenge and much more strongly depressed by the simultaneous administration of both antibodies . ^^^ Taken together , these results indicate that IFN gamma and TNF alpha play an essential role in acquired resistance during the early phase of S . typhimurium infection . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mice were infected intravenously with a sublethal dose of Listeria monocytogenes cells and then levels of tumor necrosis factor ( TNF ) , interleukin 6 ( IL 6 ) , and gamma interferon ( IFN gamma ) in the bloodstreams , spleens , and livers were monitored . ^^^ Endogenous production of neither IL 6 nor IFN gamma was inhibited in rabbit anti recombinant mouse TNF alpha antibody treated mice . ^^^ Similarly , production of TNF and IL 6 did not decrease in anti mouse IFN gamma MAb treated animals , but TNF production was augmented in these animals . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of quiescent primary human fibroblasts with tumor necrosis factor ( TNF ) alpha , TNF beta , interleukin 1 , interferon ( IFN ) alpha , IFN beta , or IFN gamma induced Egr 1 mRNA . ^^^ In primary human fibroblasts TNF alpha and TNF beta were mildly mitogenic and IFN alpha and IFN gamma were growth inhibitory . ^^^ Kinetic measurements of Egr 1 gene expression showed that TNF alpha , TNF beta , and IFN gamma increased the cellular concentration of Egr 1 mRNA within 30 min . ^^^ Despite similar Egr 1 mRNA induction , the amount of Egr 1 protein translated in IFN alpha and IFN gamma treated cells was lower than in those treated with TNF alpha and TNF beta , and highest in the EGF treated primary human fibroblasts . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The involvement of IFN beta in effecting the antiproliferative activity is suggested by ( 1 ) the ability of monospecific anti IFN beta mAB , but not anti TGF beta , anti IL 1 , anti TNF alpha , or anti IFN gamma , to neutralize the antiproliferative activity in the M phi c supernatants and ( 2 ) the ability of purified IFN beta to effect a similar inhibition of cell proliferation ( i . e . , G 1 arrest without inhibition of interleukin production ) . rTNF alpha and rIFN gamma could not effect such an inhibition of cell proliferation and did not synergize with IFN beta in producing such an antiproliferative effect . ^^^ These data suggest that the M phi c can be induced to produce inhibitory levels of cytostatic cytokines by a TNF alpha autocrine loop that is IFN gamma dependent . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have recently shown that the adhesiveness of cultured rat lymph node and PP HEV cells for thoracic duct lymphocytes can be increased significantly by pretreatment with TNF alpha , IFN gamma , and IL 4 . ^^^ In addition , TGF beta completely abrogated the increased adhesiveness of PP HEV cells normally induced by TNF alpha or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) failed to affect Con A mitogenesis of splenic T cells , even in combination with IFN gamma . ^^^ Tumor necrosis factor alpha ( TNF alpha ) failed to affect Con A mitogenesis of splenic T cells , even in combination with IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha , in combination with other known stimulators of luteal PG synthesis , interleukin 1 beta ( 2 . 5 ng / ml ) or interferon gamma ( IFN gamma , 100 U / ml ) , had synergistic effects on PGF 2 alpha production ( greater than 50 fold above control , P less than 0 . 05 ) whereas interferon alpha ( 1000 U / ml ) significantly suppressed TNF alpha stimulated PGF 2 alpha production . ^^^ Luteal cell numbers were significantly reduced by IFN gamma but not by TNF alpha alone . ^^^ However , the combination of TNF alpha + IFN gamma was extremely cytotoxic ( only 20 % of cells maintained as compared to control ) . ^^^ Finally , TNF alpha ( 100 ng / ml ) enhanced the expression of Class 1 major histocompatibility complex antigens on cultured bovine luteal cells but did not alter IFN gamma induction of Class 2 major histocompatibility complex antigens . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The induction of relatively high levels of gamma interferon ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) was also observed in responses to these same three antigen fractions . ^^^ In contrast , the proliferative , IFN gamma , and TNF alpha responses of patient lymphocytes to antigens with a molecular mass greater than 60 kDa were uniformly low . ^^^ The observed association between antigen induced proliferation and IFN gamma and TNF alpha production may be indicative of potential disease limiting immune effector activities which have developed during infection . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Before and after treatment , the following were studied : ( 1 ) serum levels of antibodies against GM 1 , asialo GM 1 ( aGM 1 ) , GM 2 and GD1b ; ( 2 ) serum levels of interleukin ( IL ) 1 beta , IL 2 , soluble IL 2 receptor ( sIL 2R ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) ; ( 3 ) IL 1 beta and TNF alpha production by peripheral blood monocytes ( PBMO ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The inhibition of Ad 2 replication in A 549 cells by IFN gamma and TNF alpha is caused , therefore , by a block in the maturation of Ad 2 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha , IFN gamma , and granulocyte / macrophage colony stimulating factor mRNAs were found to be upregulated after application of allergens , irritant , and tolerogens , but class 2 major histocompatibility complex 1 A alpha , IL 1 alpha , IL 1 beta , IFN induced protein 10 , and macrophage inflammatory protein 2 mRNAs were upregulated only after allergen painting . ^^^ Depletion of specific cell populations demonstrated that Langerhans cells were the primary source of the IL 1 beta and class 2 major histocompatibility complex 1 A alpha mRNAs , keratinocytes were the primary source of TNF alpha , IL 1 alpha , IFN induced protein 10 , and macrophage inflammatory protein 2 , and infiltrating T lymphocytes were the source of IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When TNF alpha was combined with recombinant interferon gamma ( IFN gamma ) , further upregulation of EGF R was observed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cell shape changes induced by SeLPS ( 10 micrograms / ml ) were noticeable by 24 hours and reached a maximum by 72 hours , thus paralleling the effects produced by the recombinant cytokines interferon gamma ( IFN gamma ) ( 10 ( 3 ) U / ml ) , tumor necrosis factor ( 10 ( 2 ) U / ml ) , and interleukin 1 ( 5 to 10 U / ml ) . ^^^ Conditioned medium generated over a 12 hour period following a 24 hour SeLPS / IFN gamma stimulation did not contain measurable cytokine antigens , nor did it induce biologic responses associated with interleukin 1 , IFN gamma , and tumor necrosis factor alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment twice a week from 4 weeks of age with OK 432 injected mouse serum , which contained endogenous TNF ( 75U ) , but not IL 1 , IL 2 and interferon gamma ( IFN gamma ) activity , reduced the intensity of insulitis and significantly inhibited the cumulative incidence of diabetes by 28 weeks of age in NOD mice , as compared with the incidence in non treated mice ( P less than 0 . 01 ) and in mice treated with control serum ( P less than 0 . 02 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this work , we examined the effects of tumour necrosis alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) on expression of the Fc gamma IBS in HT 29 N2 colonic cancer cells , which differentiate readily into goblet cells containing the binding site when grown in galactose containing medium . ^^^ By contrast with TNF alpha , IFN gamma ( 1 ) did not affect the proportion of cells expressing the Fc gamma IBS , ( 2 ) decreased the viability of the cells , and ( 3 ) increased cell proliferation . ^^^ Additional evidence of specificity of the TNF alpha effect on the Fc gamma IBS was that TNF alpha did not affect expression of the polymeric immunoglobulin receptor ( secretory component ) , whereas IFN gamma increased it . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , TNF alpha injected together with p 183 , a peptide known to preferentially stimulate Th 2 cells and disease exacerbation in H 2d mice , activates spleen and lymph node cells secreting more interferon gamma ( IFN gamma ) and less interleukin 4 ( IL 4 ) and induces a modest but significant degree of resistance against L . major infection in highly susceptible BALB / c mice . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Herein we demonstrate that continuous infusion of either TNF alpha or IFN gamma ( 10 ( 4 ) U / day ) via osmotic micropumps leads to an increased resistance of mice infected with a lethal dose ( 10 ( 7 ) ) of M . tuberculosis H37Rv , associated with a decreased microbial growth in all target organs . ^^^ This result was reinforced by the finding that infusion of antibodies against TNF alpha or IFN gamma greatly enhanced susceptibility of naive mice to tuberculosis . ^^^ In a final set of experiments , using neutralizing antibodies , we show that IFN gamma , not TNF alpha is involved in determining acquired resistance against murine tuberculosis , as seen by the fact that acquired immunity is resistant to anti TNF alpha antibodies , yet sensitive to anti IFN gamma antibodies . ^^^ This suggests a role for both IFN gamma and TNF alpha in determining innate resistance whereas IFN gamma may be the mediator of the anamnestic response . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The medium in which LPS triggered adherent peritoneal exudate cells were incubated was examined for the presence of tumor necrosis factor ( TNF ) , gamma interferon ( IFN gamma ) , and the soluble mediators that induce mitochondrial respiratory inhibition in tumor cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of sPEL with TA 2 inhibited sPEL migration to DTH , LPS , poly 1 : C , IFN gamma , IFN alpha / beta , and TNF alpha by 35 to 65 % and their accumulation in PLN by 50 % . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Utilizing the techniques of specific mRNA amplification by the polymerase chain reaction ( PCR ) as well as Northern blotting , we analyzed the ex vivo mRNA expression of gamma interferon ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 beta ( IL 1 beta ) , and transforming growth factor beta 1 ( TGF beta 1 ) in the peripheral blood of HAM / TSP and ATL patients as well as asymptomatic seropositive carriers . ^^^ IFN gamma , TNF alpha , and IL 1 beta transcripts were up regulated in patients with HAM / TSP and seropositive carriers when compared to their levels in ATL and normal controls . ^^^ Collectively these results suggest that induction of IFN gamma , TNF alpha , and IL 1 beta in HAM / TSP may initiate an inflammatory cascade with subsequent events leading to immune mediated destruction of the central nervous system in these patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We hypothesized that the toxicity was caused by the induction and release of endogenous cytokines such as tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) . ^^^ We measured the serum levels of TNF alpha and IFN gamma in IL 2 treated melanoma patients and attempted a correlation with clinical toxicity . ^^^ Serum TNF alpha and IFN gamma levels were measured by enzyme linked immunosorbent assay . ^^^ The kinetics and magnitude of TNF alpha production , however , were not predictable and did not correlate with either IFN gamma or toxicity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Susceptible BALB / c and resistant A / J mice were infected by the intravenous route with 10 ( 7 ) Mycobacterium lepraemurium ( MLM ) , and mortality was followed in controls and in experimental animals infused i . p . with interleukin 2 ( IL 2 ) , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Growth of murine natural killer cells from bone marrow in vitro : role of TNF alpha and IFN gamma . ^^^ Results show that mRNAs specific for tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) are detectable within hours in BM cultures supplemented with IL 2 and IL 1 alpha , and that the amount is higher when both IL 2 and IL 1 alpha are present . ^^^ Antibodies directed against TNF alpha and IFN gamma abrogate the NK cell development , indicating that these CKs play an essential role . ^^^ Furthermore , pretreatment of BM cells with TNF alpha or IFN gamma before culturing with IL 2 , enhances IL 2 responsiveness and NK cell growth . ^^^ These results suggest that induction of cytokines production may be important for growth of NK cells from BM precursors and that the synergistic effect of IL 1 alpha could be due , at least in part , to increased TNF alpha and IFN gamma production . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the cytotoxic effects of tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) on rat hepatocytes in culture . ^^^ TNF alpha and a combination of TNF alpha and IFN gamma exerted a cytotoxic effect , whereas IFN gamma showed no significant cytotoxicity when assessed by neutral red assay and by measuring LDH activity in culture medium . ^^^ Under phase contrast microscopy , the number of apoptotic cells increased with the addition of either TNF alpha or IFN gamma , and markedly with the addition of both . ^^^ DNA extracted from apoptotic cells cultured with TNF alpha and IFN gamma was fragmented , and a set of bands of the ' 200 bp ladder ' , which is characteristic of the DNA of apoptotic cells , was observed in agarose gel electrophoresis . ^^^ TNF alpha killed cultured rat hepatocytes by increasing apoptosis , and this effect was potentiated by the addition of IFN gamma , which by itself was also weakly cytotoxic . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There appears to be an overlap in signalling mechanisms of IFNs and TNF as two of the IFN inducible genes , 1 8 and 6 16 are also induced by TNF . mRNA 1 8 was induced by both IFN alpha ( type 1 ) and IFN gamma ( type 2 ) . ^^^ We conclude that TNF potentiates the cytotoxic effects of TAM in MCF 7 cells and that the three cytokines IFN alpha , IFN gamma , and TNF share some pathways that lead to specific induction of some cytokine responsive genes . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The objective of this study was to determine whether transforming growth factor beta ( TGF beta ) can inhibit the destruction of islet cells by either spleen cells from acutely diabetic BB / Wor rats , or by the cytokines tumor necrosis factor ( TNF ) and interferon gamma ( IFN gamma ) . ^^^ Similarly , TNF and IFN gamma have been shown to act synergistically to destroy islet cell monolayers . ^^^ TGF beta ( 10 ng / ml ) was also shown to significantly inhibit the TNF and IFN gamma induced lysis of islet cell monolayers . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The adhesiveness of LN HEV cells for thoracic duct lymphocytes ( TDL ) was significantly increased in a dose and time dependent manner by pretreatment of the HEV cells with tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) or interleukin 4 ( IL 4 ) . ^^^ Furthermore , incubation of LN HEV cells with suboptimal doses of TNF and IL 4 , IFN gamma and IL 4 , or TNF alpha and IFN gamma increased significantly the endothelial adhesiveness . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The macrophages released more superoxide ( O 2 ) , TNF and interleukin 1 ( IL 1 ) on LPS triggering , and the releases of these compounds were further increased by addition of recombinant interferon gamma ( IFN gamma ) to the medium . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Western blotting analysis of the supernatant fluid from the culture of the spindle shaped cells showed the presence of several cytokines ; M CSF , IFN gamma , TNF alpha , which were known to show chemotactic , differentiation inducing and activating effects on macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The genes for interferon ( IFN ) alpha , IFN gamma , IL 1 beta , IL 6 , and TNF alpha were transcribed at readily detectable levels both in liver biopsies from individuals with normal liver function and in samples of normal viable liver taken for transplantation . ^^^ The reduced expression of IL 1 , IL 6 , and TNF mRNAs appeared to be a specific effect and not due to a general reduction in RNA synthesis as the IFN alpha , IFN gamma and actin mRNAs were expressed at similar levels in both normal and diseased livers . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The immunomodulatory cytokines , including interleukin 1 ( IL 1 ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) , are also known to modulate the growth response in nonimmune cells and regulate cellular metabolism by inducing expression of genes encoding biologically active molecules . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Sensitivity to growth inhibition by IL 6 is independent from that of IFN beta 1 , IFN gamma or TNF . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Protein A and other surface components of Staphylococcus aureus stimulate production of IL 1 alpha , IL 4 , IL 6 , TNF and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro they promote T cell mitogenesis and in vivo , the self limited cytokine release ( including TNF , IFN gamma , IL 2 , IL 3 , IL 6 ) observed following the first anti CD 3 injection , is responsible for an acute clinical syndrome . ^^^ In particular , this model allowed the description of regulatory pathways existing between TNF and IFN gamma , which in turn regulate IL 3 and IL 6 release . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Nor was it affected by T cell activation signals or by such cytokines as tumor necrosis factor alpha ( TNF alpha ) , interleukin 6 ( IL 6 ) , interferon gamma ( IFN gamma ) , and interferon alpha ( IFN alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Supernatants from cultures of targeted PBL , or from PBL grown on anti CD 3 coated flasks , blocked the growth of tumor cells in the absence of added effector cells , and antibodies against TNF alpha and IFN gamma reversed the inhibition of tumor growth , but had no effect upon cytolysis mediated by targeted by PBL . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found that CD4+ , CD45RA ( or CD45RO+ ) T cells consistently produced the most IL 2 , IFN gamma , and TNF alpha after mitogen stimulation for 2 days . ^^^ Together , these data indicate that CD 4 , CD45RA ( or CD45RO+ ) T cells in peripheral blood are the major producers of IL 2 , IFN gamma , and TNF alpha following short term mitogen stimulation , and that phenotypically defined peripheral blood T cell subsets do not maintain a distinct pattern of cytokines during extended culture periods . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have studied the ability of subpopulations of activated thymocytes to produce four cytokines ( IL 2 , IL 4 , IFN gamma and TNF alpha ) which are believed to play roles in T cell development . ^^^ CD 3 4 8 thymocytes produced IL 2 , IFN gamma , and TNF alpha ( but not IL 4 ) when activated by calcium ionophore + PMA and IL 1 . ^^^ CD 3 4 8 IL 2R+ thymocytes only produced small amounts of IL 2 when activated with calcium ionophore + PMA + IL 1 , whereas CD 3 4 8 IL 2R thymocytes did not require IL 1 to produce IL 2 , IFN gamma , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Supernatants were assayed for tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) and CD 4 enriched MLC populations produced high levels of these cytokines . ^^^ The GVHR produced by the supernatant alone could be inhibited by both anti IFN gamma and anti TNF alpha polyclonal antibodies . ^^^ The results suggest that TNF alpha and IFN gamma are directly involved in tissue damage during graft versus host disease in allogeneic transplant in man . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The latter factor does not appear to be a previously described lymphokine , since PMA and A 23187 activated NS cells secrete IL 3 , TGF beta , IFN gamma , GM CSF , and TNF alpha , but not IL 1 , IL 2 , IL 4 , IL 5 , IL 6 , IL 7 , nor IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A subsequent minor late phase ( greater than or equal to 12 h ) of IL 1 mRNA was induced independently of cell contact in monocytes that received only soluble factors generated during anti CD 3 mitogenesis and was temporally associated with the appearance in culture supernatants of the late phase IL 1 inducing cytokines , IL 2 , IFN gamma , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have studied the influence of tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) on the development of cytotoxic T lymphocytes ( CTL ) against melanoma in mixed lymphocyte tumour cultures ( MLTC ) . ^^^ IFN gamma at 10 ( 4 ) U / ml and combinations of TNF alpha plus IFN gamma at 10 ( 2 ) 10 ( 3 ) U / ml promoted the proliferation more variably . ^^^ MLTC generated with rIL 2 showed a predominance of CD8+ cells , while 2 weeks of culture in the presence of IFN gamma at 10 ( 4 ) U / ml , or with IFN gamma and TNF alpha at 1 10 10 ( 2 ) 10 ( 3 ) U / ml , favoured the emergence of CD4+ cell populations . ^^^ The cytotoxic activity of the lymphocytes generated in these MLTC showed a consistent decline of K 562 cytotoxic activity following exposure to the combination of IFN gamma and TNF alpha . ^^^ These results suggest that TNF alpha and IFN gamma influence the activation , phenotypic , and functional outcome of MLTC generated CTL , and may account for the phenotypic variations observed in T cell populations generated in vitro . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study we have used the reverse hemolytic plaque assay to investigate either the direct effects of the protein kinase activator , phorbol myristate acetate ( PMA ) , or exposure to recombinant human interleukins 2 , 4 , and 6 ( IL 2 , IL 4 , and IL 6 ) tumour necrosis factor alpha ( TNF alpha ) and basic fibroblast growth factor ( bFGF ) on the release of IFN gamma by individual , immunoidentified NK cells isolated from peripheral blood . ^^^ Whilst TNF alpha had no significant effect on IFN gamma release by NK cells , a 6 hr exposure to IL 2 or PMA stimulated an increase in the amount secreted per single cell . ^^^ These studies demonstrate that ( 1 ) both the spontaneous and stimulated release of IFN gamma by NK cells can be visualized and quantified at the single cell level using this sensitive immunoassay , and ( 2 ) bFGF and interleukins 2 , 4 , and 6 , but not TNF alpha , are potent stimulants of IFN gamma secretion by CD 3 / CD16+ NK cells . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The IFN gamma blockade also inhibited IFN gamma , IL 2 , and TNF alpha release during MLC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A major step forward in the past few years has been the realization that shed extracellular domains of cytokine receptors are the most common natural cytokine inhibitors , described for TNF , IFN gamma , IL 2 , IL 4 , IL 7 , for example . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Membrane associated tumor necrosis factor ( TNF ) and soluble TNF were compared as to their lytic activities , and as to the kinetics of their expression by macrophages activated with LPS and / or IFN gamma in the presence or absence of cycloheximide . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , low doses of recombinant TNF alpha and TNF beta were found to potentiate the suppressive activity of exogenous IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The first antibody injection consistently induced an initial monocyte dependent T cell activation with subsequent release of both monocyte and T cell derived cytokines [ mainly tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , interleukin ( IL ) 2 , IL 3 and IL 6 ) into the circulation . ^^^ We report here that a single injection of anti TNF mAb prior to anti CD 3 administration not only neutralizes the biological activity of TNF but also strongly affects the release of other cytokines , with notably an up regulation of IFN gamma release and a down regulation of IL 3 and IL 6 release . ^^^ Conversely , pretreatment with anti IFN gamma mAb increases IL 3 and IL 6 production but does not affect TNF levels . ^^^ Taken together , these data point to a pivotal role of IFN gamma in the anti CD 3 induced cytokine cascade and reveal new regulatory pathways between TNF and IFN gamma . ^^^ With regard to the clinical implications of these findings , as anti TNF mAb prevents anti CD 3 induced sickness in mice , whereas anti IFN gamma does not , such a therapeutic approach might be of value in OKT 3 treated patients . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We also found that LPS is a potent stimulator of monokines : IL 6 , tumour necrosis factor alpha ( TNF alpha ) , and IL 1 beta , as determined by radioimmunoassay method , and interferon gamma ( IFN gamma ) , IL 2 , TNF alpha and IL 1 alpha , as determined by ELISA method , in peripheral blood mononuclear cells ( PBMC ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Other cytokines such as IL 1 , IL 2 , IL 3 , IFN alpha , IFN gamma and TNF alpha had no significant effects on CD 23 expression . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Absence of lysis of tumour clone 2 / 51 by supernatants correlated with resistance of the same tumour clone to lysis by recombinant IFN gamma plus TNF alpha . ^^^ Antibodies to TNF alpha and , to a lesser extent , to IFN gamma , reduced significantly the 96 hour lysis of Me2 / 9 and Me2 / 10 , two of the tumour clones killed in long term but not in short term assays . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using a combination system of Transcription and PCR , we examined gene expression of TNF alpha , TNF beta and other cytokines ( IL 1 , G CSF , IL 3 and IFN gamma ) in embryonal carcinoma cell lines ( PCC 3 , PCC 4 , ECA 2 and F 9 ) and trophoblast cell line ( PL / B6 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The L 929 cell cytotoxicity assay and an ELISA were used to show that the amount of TNF alpha present was less in medium from IFN gamma treated cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The treatment of macrophages with IFN gamma led to an increase in the level of RNA for both TNF and 1 A beta . ^^^ The increase in expression of 1 A beta and TNF , induced by IFN gamma , was blocked by naphthalenesulfonamide or phenothiazine ( trifluoperazine ) but was not affected by the addition of isoquinolinesulfonamide or sphingosine . ^^^ These results suggest that the induced expression of 1 A beta and TNF by IFN gamma is mediated by a pathway that is protein kinase C independent . ^^^ We observed that when both IFN gamma and PMA were added to the macrophages , the level of RNA for TNF increased to a higher level than the level seen when either agent alone was added to the cells . ^^^ IFN gamma induces the expression of the genes for MHC class 2 1 A beta and tumor necrosis factor through a protein kinase C independent pathway . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both TNF and TNF receptors are detectable on monocyte membranes by FACS analysis , and the levels of each are modulated by treatment with IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor ( TNF ) synergizes with interferon ( IFN gamma ) in the blockade of HSV 1 replication . ^^^ Antibodies against IFN beta block this synergism , implying a role of IFN beta in the antiviral activity of TNF plus IFN gamma . ^^^ Our results are in accordance with the idea that TNF induces IFN beta 1 and that both cytokines must be present in the culture medium to synergize with IFN gamma in order to inhibit HSV 1 replication . . ^^^ Tumor necrosis factor ( TNF ) synergizes with interferon ( IFN gamma ) in the blockade of HSV 1 replication . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Because IFN gamma is the main cytokine activating macrophages and TNF cooperates in this activation , we assessed TNF binding capacity during the course of murine macrophage activation by IFN gamma . ^^^ Preincubation of macrophages with IFN gamma prevented the binding of TNF to receptors . ^^^ IFN gamma also reduced specific TNF binding to preexisting receptors ( 50 % inhibition in 3 h ) , but IFN gamma did not change the internalization rate of TNF . ^^^ These studies showed that the number of TNF receptors increased on macrophages vs maturation in culture and was negatively controlled by IFN gamma . . ^^^ IFN gamma reduces specific binding of tumor necrosis factor on murine macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Uterine or peritoneal leukocytosis may inhibit development via toxic effects of lymphokines / monokines ( IL 2 , IL 1 ? , IFN gamma , TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The decrease in responsiveness was not caused by cell death , since in the case of TNF alpha it was fully reversible by interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Transcription of IL 6 and TNF beta started on day 4 , whereas IFN gamma mRNA reached its maximum level on day 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Melanocytes expressed mRNA for IL 8 and MCAF , when stimulated with either IL 1 alpha or TNF alpha , but not when stimulated with IL 6 , IFN gamma , or LPS alone . ^^^ When IFN gamma was added to melanocyte cultures stimulated with suboptimal doses of TNF alpha there was a synergistic increase in secreted IL 8 protein and monocyte chemotactic activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cells stained for interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) , which appeared to be CD3+ cells and CD14+ cells , respectively , were seen scattered around granulomas . ^^^ Lymph nodes of patients with sarcoidosis contained significantly higher amounts of IFN gamma , TNF alpha and IL 1 beta than control lymph nodes . ^^^ The levels of IFN gamma and TNF alpha in extracts of lymph nodes were correlated with the histological status of the granulomas . ^^^ IFN gamma was detected in all stages , while the highest level of TNF alpha was obtained from lymph nodes containing many active granulomas . ^^^ These results suggest that quantitative analyses of IFN gamma and TNF alpha in sera and lymph nodes may serve to elucidate the pathophysiology of sarcoidosis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We examined the antiviral activity against the neurotropic pseudorabies virus ( PRV ) of interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) , and combinations of these cytokines , as compared to that of IFN beta , in rat nervous tissue cells . ^^^ At a low m . o . i . , IFN gamma and IFN beta inhibited viral plaque formation in all cell types ; TNF alpha was effective only in astrocytes but showed synergy with IFN gamma . ^^^ At a higher m . o . i . , IFN beta inhibited yields of infectious virus more effectively than IFN gamma , whereas TNF alpha had no effect on virus yields and was only marginally synergistic with the antiviral activity of IFN gamma . ^^^ Our results show that both IFN gamma and IFN beta can induce a state of antiviral resistance in neural cells whereas TNF alpha is effective only in astrocytes at low m . o . i . ; they suggest an antiviral role of cytokines in the immune response to virus infections of the CNS . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human myelogenous leukemic cell lines ( ML 1 , U 937 , THP 1 ) were induced to differentiate by treatment with various cytokines such as tumor necrosis factor ( TNF ) , interferon gamma ( IFN gamma ) and interleukin 6 . ^^^ Both normal and patient sera , at effective concentrations , only slightly affected the binding of 125I TNF to ML 1 cells , but any of these sera significantly inhibited the binding of 125I IFN gamma to U 937 cells or to THP 1 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When compared with the effects of TNF alpha similar results were seen although IFN gamma and TNF alpha were effective at 10 fold less the concentration of lymphotoxin . ^^^ Enhancement of neutrophil mediated parasite killing by lymphotoxin and IFN gamma : comparisons with tumor necrosis factor effects . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The growth inhibition induced by rhIL 4 in the Ph 1 positive ALL cells was not abrogated by the addition of antibodies against either IFN gamma or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The T cell help for IL 1 production could not be replaced by a panel of T cell derived recombinant lymphokines added to SEA stimulated monocytes , including IFN gamma and TNF , indicating the participation of cell membrane bound ligands or hitherto unidentified soluble mediators . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
ICAM 1 was up regulated on TMF in culture by recombinant IFN gamma , IL 1 and TNF alpha and was down regulated by dexamethasone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The association of IL 1 beta and TNF alpha showed an additive effect on both parameters , whereas addition of IFN gamma to either monokine reduced the proliferation and increased PGE 2 release . ^^^ Incubation with a crude T cell supernatant or a mixture of cytokines including IL 1 beta , TNF alpha and IFN gamma enhanced synovial cell growth and PGE 2 production as compared to the effect elicited by each single cytokine . ^^^ Nevertheless , the monokines IL 1 beta and TNF alpha both exert agonistic effects on synovial cell activation , thus contributing to cartilage damage in RA , whereas IFN gamma , IL 6 or IL 2 may rather play a regulatory role . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PBMC were cultured for 7 days in cell wells containing no cytokines , tumor necrosis factor ( TNF ) , gamma interferon ( IFN gamma ) , 1 , 25 dihydroxycholecalciferol ( vitamin D 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , or interleukin 2 ( IL 2 ) and were then challenged for 24 h with a fixed number of CFU of C . neoformans . ^^^ The number of CFU increased in wells containing no cytokines , TNF , IFN gamma , or vitamin D 3 and remained about the same in wells containing GM CSF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Effects of TNF alpha on the expression of class 2 MHC molecules in macrophages induced by IFN gamma : evidence for suppression at the level of transcription . ^^^ The strong expression of such molecules induced by interferon gamma ( IFN gamma ) was , however , suppressed moderately by TNF alpha . ^^^ Furthermore , the locus of control appears to be transcriptional ; in nuclear run on assays , TNF alpha suppressed the IFN gamma induced enhancement of transcription for the murine beta chain of 1 A ( 1 A beta . ) . ^^^ Taken together the data suggest that TNF alpha has little effect on class 2 MHC genes and surface expression in murine peritoneal macrophages , that TNF alpha is a modest suppressant of such molecules when their levels are raised by IFN gamma , and that these suppressive effects are mediated at the level of transcription . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A panel of 12 squamous cell carcinoma of the head and neck ( SCCHN ) cell lines has been used to determine sensitivity of tumor cells to cytokines , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and interferon alfa ( IFN alpha ) in vitro . ^^^ The combination of IFN gamma and TNF alpha had a synergistic antiproliferative effect on eight cell lines and an additive effect on two cell lines . ^^^ These in vitro studies indicate that combinations of IFN gamma and TNF alpha or IFN alpha and TNF alpha may be more growth inhibitory against squamous cell carcinoma of the head and neck and at lower doses than each of these cytokines used singly . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha has been found both to enhance and also to inhibit IFN gamma induced MHC class 2 expression . ^^^ In the present studies , the effect of TNF alpha on IFN gamma induced MHC class 2 expression was tested in various cell lines . ^^^ On the basis of the data , we propose that , depending on the stage of differentiation and maturation of the cells , TNF alpha might synergize or antagonize the affects of IFN gamma on the regulation of MHC class 2 expression . ^^^ Thus , in immature cells such as HL 60 or THP 1 , TNF alpha enhances IFN gamma induced class 2 expression . ^^^ However , when differentiation was induced in these cells by TPA or IFN gamma , the additive effect of TNF alpha on the IFN gamma induced DR expression was eliminated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , we found that PSK has a function to enhance rIL 2 induced IFN gamma and TNF alpha production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Recombinant rat IFN gamma ( 2000 U / rat . 9 days ) more than tripled the production of TNF alpha by macrophages from hypophysectomized rats ( 343 vs . 112 U / mg protein ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Incubation with IFN gamma and TNF alpha ( 250 U / ml ) in combination resulted in increased production of MCAF protein . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study examines the possible relationship between TNF alpha and IFN gamma , as components of host defense mechanisms , and CFN . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Many cytolytic and noncytolytic CD8+ TIL cultures specifically secreted interferon gamma ( IFN gamma ) and tumor necrosis factor when stimulated with tumor cells in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Blood was drawn immediately before , and at 2 and 24 hours after the first dose of OKT 3 for determination of interleukin 2 ( IL 2 ) , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) levels , and flow cytometric analysis of T cell subsets . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Marked elevation was found of TNF , IFN gamma , IL 2 , and IL 2R in BALF obtained from the grafted lungs in group 1 and group 2 dogs . ^^^ Our study suggests that serially measuring BALF levels of TNF , IFN gamma , IL 2 , and IL 2R may serve as a useful means in monitoring the immunologic status of canine lung allografts and in the early detection of lung allograft rejection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 6 , TNF alpha , and IFN gamma RNA transcripts were not detected in any of the sections of normal human kidneys . ^^^ In contrast , in kidney transplant patients with stable renal function no significant labeling was observed with the IL 6 , TNF alpha , or IFN gamma probes . ^^^ The expression of the interleukin 6 , tumor necrosis factor alpha , and interferon gamma ( IFN gamma ) genes was studied in human renal biopsies from individuals without evidence of kidney disease and from patients undergoing acute renal allograft rejection using a method of in situ hybridization capable of detecting 1 5 copies of a specific cellular messenger RNA in individual cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have recently demonstrated TNF alpha expression from astrocytes induced by lipopolysaccharide ( LPS ) , interferon gamma ( IFN gamma ) , and interleukin 1 beta ( IL 1 beta ) . ^^^ Astrocytes secrete TNF alpha in response to LPS alone , and can be primed by IFN gamma to enhance LPS induced TNF alpha production . ^^^ IFN gamma and IL 1 beta , cytokines known to be present in the CNS during neurological disease states , do not induce TNF alpha production alone , but act synergistically to stimulate astrocyte TNF alpha expression . ^^^ Astrocytes from BN rats express TNF alpha mRNA and protein in response to LPS alone , yet IFN gamma does not significantly enhance LPS induced TNF alpha expression , nor do they express appreciable TNF alpha in response to the combined stimuli of IFN gamma / IL 1 beta . ^^^ In contrast , astrocytes from Lewis rats express low levels of TNF alpha mRNA and protein in response to LPS , and are extremely responsive to the priming effect of IFN gamma for subsequent TNF alpha gene expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We recently reported that lymphokine activated killer ( LAK ) cells were stimulated to release both interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) when stimulated by a variety of tumor cells . ^^^ In this paper , we provide further information on the nature of the signals , provided by the tumor cells ( K 562 erythroleukemia ) , that stimulate LAK cells to secrete IFN gamma and TNF alpha . ^^^ Beads coated with K 562 membranes stimulated LAK cells to release IFN gamma and TNF alpha . ^^^ The glycoproteins that stimulate LAK cells to secrete IFN gamma and TNF alpha were further enriched by their ability to bind concanavalin A ( Con A , Jack Bean ) . ^^^ To determine if the tumor associated molecules that stimulate LAK cells to release IFN gamma and TNF alpha are also the molecules involved in mediating tumor cell lysis , we tested the ability of the Con A binding and nonbinding proteins to inhibit the LAK cell mediated lysis of K 562 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have examined the antitumor effects of rat gamma interferon ( IFN gamma ) and human tumor necrosis factor alpha ( TNF ) against androgen dependent and independent Dunning rat prostatic tumors . ^^^ In vitro studies , using the double layer soft agar assay , showed a very limited antiproliferative activity of the drugs in the dose range tested ( 1 1000 units IFN gamma and / or 1 1000 ng TNF / dish ) . ^^^ For in vivo studies IFN gamma and TNF were administered s . c . , peritumorally . ^^^ IFN gamma was given 3 times / week , 8 , 000 or 80 , 000 units / rat , and TNF 5 times / week , 10 or 100 micrograms / rat . ^^^ IFN gamma and TNF monotherapy were not significantly effective in inhibiting tumor growth , except for IFN gamma against the androgen independent MatLyLu tumor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro studies shows that recombinant tumour necrosis factor ( TNF ) alpha and beta , and interferon gamma ( IFN gamma ) can enhance HIV replication , and peripheral blood mononuclear cells ( PBMC ) infected with HIV in vitro secrete high levels of the same cytokines . ^^^ We demonstrate that asymptomatic seropositives in CDC group II / III as well as patients who have progressed to CDC group 4 of the disease proliferate efficiently to anti CD 3 antibody , recombinant interleukin 2 ( rIL 2 ) , phytohaemagglutinin ( PHA ) , PHA plus phorbol 12 , 13 dibutyrate ( PMA ) but secrete significantly ( P less than 0 . 05 ) higher amounts of TNF alpha , TNF beta and IFN gamma compared with controls in response to the same stimulants . ^^^ We also show a difference between group II / III and group 4 patients with the latter secreting more TNF alpha and IFN gamma . ^^^ The kinetics of TNF alpha and beta , and IFN gamma production was stimulus dependent with overall levels varying in time for each stimulus . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 4 acts as a homeostatic regulator of IL 2 induced TNF and IFN gamma . ^^^ IL 2 stimulated lymphocytes also secrete two cytokines , tumour necrosis factor ( TNF ) and gamma interferon ( IFN gamma ) , which contribute to effector function and which may themselves recruit fresh , cytokine secreting effector cells . ^^^ We have now investigated whether the IL 4 induced is able to homeostatically regulate secretion of the TNF and IFN gamma . ^^^ IL 2 induced high levels of TNF and IFN gamma secretion in both groups . ^^^ The addition of recombinant IL 4 to these IL 2 stimulated cultures lead to significant inhibition of IFN gamma and TNF production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The reduced sensitivity to IFN gamma or LPS for expression of IP 10 , JE , and KC mRNA as compared with TNF alpha or D 3 mRNA suggests that this distinct pattern of regulation may be restricted to members of these two related cytokine gene families that exhibit cell type specific chemoattractant activity . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) and interleukin 1 beta ( IL 1 ) were determined in supernatants from stimulated and unstimulated cultures with or without addition of indomethacin . ^^^ Indomethacin enhanced IFN gamma release and spontaneous and induced TNF alpha secretion in all groups but stimulated IL 1 only in irradiated patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
For this purpose BM T enriched cells from 11 AA patients and 9 normal individuals were incubated with ALG ( 0 1000 micrograms / ml ) or PHA ( 0 % 10 % ) for 1 day , and the supernatants were tested for suppression / enhancement of granulocyte macrophage colony forming unit ( CFU GM ) growth and for release of granulocyte macrophage colony stimulating factor ( GM CSF ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) assayed with the enzyme amplified sensitivity immunoassay ( EASI ) . ^^^ The same supernatants contained detectable amounts of IFN gamma and TNF alpha ( 21 + / 6 . 7 IU / ml and 4 . 6 + / 2 . 9 ng / ml , respectively ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Because the combination of IFN gamma with lipopolysaccharide greatly enhanced macrophages ability to decrease the number of intracellular parasites , the interaction of IFN gamma with tumor necrosis factor ( TNF ) was examined . ^^^ TNF alone and the combination of TNF with IFN gamma did not have a significant effect on reducing parasite numbers below that obtained with IFN gamma alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Whereas maximal accumulation of mRNA species for IL 2 , GM CSF , TNF alpha and TNF beta did not reveal major differences between cells of T IL 2 and T IL 4 cultures , substantial differences emerged for the induction of IFN gamma and IL 3 messages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of macrophage conditioned supernatants with neutralizing monoclonal anti tumor necrosis factor ( TNF ) significantly reduced their capacity to stimulate NK cells to produce IFN gamma . ^^^ Thus , TNF was necessary but not sufficient to induce maximal IFN gamma production by NK cells . ^^^ Sonicated L . monocytogenes stimulated production of IFN gamma by NK cells that was resistant to anti TNF . ^^^ These studies demonstrated that activation of scid NK cells for secretion of IFN gamma requires two signals : TNF alpha and a second product which may be of bacterial origin and may require processing by mononuclear phagocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mf B induced a small amount of tumor necrosis factor in M theta , and this induction was markedly enhanced by IFN gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This inhibitory effect was mediated at least in part by the tumour necrosis factor alpha ( TNF alpha ) and IFN gamma secreted by the clones . ^^^ Involvement of additional factors is also suggested in blocking CML cell proliferation because this was not 100 % inhibited even by a combination of TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To gain more insight into the mechanisms involved in the modulation of killing , we evaluated the possible stimulating activity of IFN gamma plus LPS treatment on the secretion of tumor necrosis factor ( TNF ) and interleukin 1 ( IL 1 ) , two cytokines produced by activated macrophages and capable of stimulating natural effectors . ^^^ The results showed that IFN gamma plus LPS can indeed stimulate TNF and IL 1 secretion by AM and PBM from patients with COPD . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have compared the effects of interleukin 2 ( IL 2 ) or tumor necrosis factor alpha ( TNF alpha ) administration with or without interferon gamma ( IFN gamma ) on Graves ' and normal thyroid tissue xenografts in the nude mouse ( in the absence of an intact immune system ) in terms of possible functional , immunological , or histological changes . ^^^ The dosages of recombinant human IL 2 , TNF alpha , and IFN gamma given to each mouse were 250 , 800 , and 4000 U , respectively ; they were injected ip daily for 6 consecutive weeks . ^^^ There were no significant differences in thyroid function between the results in unstimulated ( control ) animals and those obtained with cytokine administration in either group of tissues , with the exception of the group receiving TNF alpha together with IFN gamma ; in this latter group , the free T 4 index declined significantly 4 6 weeks after commencement of treatment in the animals with normal thyroid tissue xenografts . ^^^ The reduction of thyroid function induced by the combination of IFN gamma and TNF alpha observed in normal thyroid tissue may be due to inhibition of thyroperoxidase and thyroglobulin gene transcription . ^^^ On the other hand , TNF alpha plus IFN gamma induced TEC HLA DR expression on both types of thyroid xenografts at death , although IL 2 alone did not induce HLA DR expression , and IFN gamma induced TEC significantly only on normal thyroid xenografts ( but not on Graves ' xenografts ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this report , we show that the macrophage activating agents , TNF alpha , IFN gamma , and LPS , are all potent inhibitors of CSF 1 stimulated murine bone marrow derived macrophage ( BMM ) DNA synthesis and increase in cell numbers . ^^^ The inhibitory effects of TNF alpha and IFN gamma do not appear to be due to endotoxin contamination in the recombinant cytokine preparations . ^^^ The inhibitory effects of TNF alpha , IFN gamma , or LPS were also seen when granulocyte macrophage CSF or IL 3 was used to stimulate BMM DNA synthesis . ^^^ The results suggest that TNF alpha , IFN gamma , and LPS appear to be inhibiting CSF stimulated proliferation by acting at a post receptor level , possibly by regulation of some critical event ( s ) in the mitogenic signaling pathway . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have identified two of four melanoma and one of seven breast carcinoma TIL cultures that specifically release granulocyte macrophage CSF , TNF alpha , and IFN gamma after autologous tumor stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The stimulatory effect was also observed in methylcellulose culture , but the addition of antibodies for G CSF , granulocyte macrophage CSF , IL 1 alpha , IL 1 beta , IL 6 , or tumor necrosis factor did not result in a decrease of the colony number , supporting further the possible direct effect of IFN gamma on progenitor growth . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor ( TNF ) and interferon gamma ( IFN gamma ) are immune modulating cytokines that exert synergistic cytotoxic effects in several types of tumor cells , including ASPC 1 human pancreatic carcinoma cells . ^^^ In the present study , we examined ASPC 1 cells for cytokine mediated modulation of TNF and IFN gamma receptors . ^^^ These data suggest that both TNF and LT upregulate IFN gamma receptors in ASPC 1 cells , but that LT is much less efficient than TNF . ^^^ Further , the TPA induced attenuation of IFN gamma receptor upregulation suggests that protein kinase C activation can regulate the TNF / LT mediated pathways involved in IFN gamma receptor upregulation . . ^^^ Tumor necrosis factor ( TNF ) and interferon gamma ( IFN gamma ) are immune modulating cytokines that exert synergistic cytotoxic effects in several types of tumor cells , including ASPC 1 human pancreatic carcinoma cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Administration of TNF alpha and IFN gamma also caused transient monocytopenia . ^^^ This study was designed to investigate acute effects of various doses of the cytokines IFN alpha , IFN gamma , Interleukin 2 and tumor necrosis factor alpha on white blood cell differential counts . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The authors used this technique to monitor changes in the expression of selected cellular proteins after exposure to specific cytokines and found that MHC class 1 molecules were augmented by interferon alpha ( IFN alpha ) and interferon gamma ( IFN gamma ) , but not by tumor necrosis factor ( TNF ) . ^^^ However , the MHC class 2 molecules were augmented by IFN gamma but not by IFN alpha or TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha was neither required for IFN gamma activity , nor effective at any concentration tested as an inhibitor of schizont development by itself in primary hepatocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using functional inhibition experiments , the activity of lipopolysaccharide ( LPS ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) in CM was investigated . ^^^ The LPS inhibitor polymyxin B and TNF alpha antibody produced a modest decrease in nitrite production , while IFN gamma antibody markedly inhibited both nitrite production and cytostasis . ^^^ Simultaneous treatment with polymyxin B , TNF alpha antibody , and IFN gamma antibody reduced EMT 6 cell nitrite production by 81 % , and cytostasis by 74 % . ^^^ By Western blot , IFN gamma and TNF alpha were shown to be present in CM . ^^^ We conclude that induction of inorganic nitrogen oxide synthesis from L arginine by the synergistic combination of IFN gamma , TNF alpha , and LPS accounts for most of the biologic activity of CM , and that IFN gamma is the major priming factor . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Trypanostatic activity of IFN gamma treated macrophages was reduced by addition of anti TNF alpha showing the participation of TNF alpha in IFN gamma mediated macrophage trypanostatic activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Few clinical responses have occurred in preliminary studies using the cytokines tumor necrosis factor alpha ( TNF alpha ) or interferon gamma ( IFN gamma ) in cancer patients . ^^^ Resistance to lysis by TNF alpha or IFN gamma in many cells is controlled by a protein synthesis dependent mechanism , such that when protein synthesis is inhibited cells become sensitive to lysis by these cytokines . ^^^ Because there is some evidence that TNF alpha and IFN gamma act through different lytic mechanisms and are opposed by different resistance mechanisms , we treated a panel of eight cell lines , five derived from human cervical carcinomas ( ME 180 , MS 751 , SiHa , HT 3 , and C 33A ) and three derived from ovarian carcinomas ( Caov 3 , SK OV 3 , and NIH : OVCAR 3 ) with both TNF alpha and IFN gamma to determine whether such combination treatment might maximize in vitro cell lysis . ^^^ Our results showed that pretreatment with IFN gamma followed by exposure to TNF alpha in the presence of protein synthesis inhibitors increased lysis of seven of the eight cell lines above that seen with either TNF alpha or IFN gamma and inhibitors of protein synthesis . ^^^ Only the cell line C 33A was resistant to lysis by TNF alpha and IFN gamma , when exposed to these agents both alone and in combination with protein synthesis inhibitors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the culture supernatants the cytokines interleukin 1 alpha ( IL 1 alpha ) , interleukin 2 ( IL 2 ) , interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) were determined by a rapid and sensitive immunoassay using various monoclonal and polyclonal antibodies . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we examined the effect of in vitro Kupffer cell activation by recombinant murine interferon gamma ( IFN gamma ) on IL 1 and TNF secretion . ^^^ IFN gamma enhanced TNF production in the presence or absence of lipopolysaccharide ( LPS ) , but suppressed IL 1 production by Kupffer cells . ^^^ Because IFN gamma also stimulated prostaglandin E 2 ( PGE 2 ) production , the effect of indomethacin , which is an inhibitor of cyclooxygenase and which inhibits PGE 2 biosynthesis , on IL 1 and TNF production by Kupffer cells was examined . ^^^ These results suggested that IFN gamma modulates the production of IL 1 and TNF by Kupffer cells through different mechanisms . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Addition of polyclonal neutralizing antibodies against both interferon gamma ( IFN gamma ) and tumor necrosis facto alpha ( TNF alpha ) to the coincubation culture completely abrogated the suppressive effect of A LAK on BFU E and CFU MIX colony growth while each neutralizing antibody used alone had intermediate effects . ^^^ Addition of neutralizing antibodies against IFN gamma and TNF alpha in preincubation failed to abrogate the suppressive effect of A LAK on BFU E colony growth , suggesting that this suppression occurs by a different mechanism than that seen in coincubation studies . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this report , we have investigated the contribution of IL 4 , TNF alpha and IFN gamma to the induction of antileishmanial macrophage activation . ^^^ When the macrophages were incubated with concentrations of IFN gamma that by themselves were insufficient for maximum cell activation , it was found that both IL 4 and TNF alpha very effectively synergized with IFN gamma for induction of antiparasitic activity . ^^^ The activation which was achieved when IFN gamma was combined with IL 4 could be blocked not only with antibodies to either of the lymphokines , but also with an antiserum specific for TNF alpha , suggesting the involvement of endogenously generated TNF alpha in this synergism . ^^^ We conclude that macrophage activation for antiparasitic activity is directed by a complex network of cytokine interactions , in which IL 4 and TNF alpha very effectively synergize positively with low levels of IFN gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In an attempt to determine the cytokines responsible for monocyte accumulation and antibacterial protection we could show that IFN gamma and TNF , both discussed as proinflammatory as well as macrophage activating cytokines , both can be produced in a T cell dependent as well as in a T cell independent manner . ^^^ The ability of listeria specific CD8+T cells to secrete TNF and IFN gamma in response to listerial antigen argues against a role of these cytokines as mediators of granuloma formation . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Some of these proteins are induced uniquely by IFN gamma , whereas others are also induced by IFN alpha , TNF , or IL 1 . ^^^ Mn SOD is induced by IFN gamma and also by TNF in all four human cell lines examined : HS 153 fibroblasts , ACHN renal carcinoma , A 549 lung carcinoma , and A 375 melanoma . ^^^ In ACHN renal carcinoma cells , Mn SOD mRNA and protein are induced synergistically by IFN gamma in combination with either TNF or IL 1 , and the induced protein is enzymatically active . ^^^ IFN gamma and TNF together induce Mn SOD mRNA by more than 100 fold relative to its level in untreated ACHN cells . ^^^ The induction of Mn SOD by IFN gamma and its synergistic induction by IFN gamma in combination with TNF and IL 1 should protect healthy cells from the toxicity of O 2 during an immune response , and may provide a mechanism for selective killing of infected cells . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To investigate the influence of gynaecological cancer on in vitro and in vivo cytokine production of peripheral mononuclear cells ( PBMC ) from 27 patients with mamma carcinoma and 29 patients with cervical cancer , we evaluated the production of interferon alpha ( IFN alpha ) , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma monotherapy ( dose range 8 80 ng / gram bodyweight ) was less effective than IFN alpha . 500 ng / gram TNF produced growth inhibition of both tumors , whereas the lower dose ( 50 ng / g ) was only effective against the PC 3 tumor . ^^^ IFN gamma and TNF combinations also showed significant antitumor effects against both tumor lines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , TNF alpha and IFN gamma , alone and synergistically , increase cell surface expression of transfected MHC class I / endogenous beta 2m heterodimers without induction of endogenous class 1 alpha chain genes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) strongly activates murine peritoneal macrophages ( M phi ) for killing of amastigotes from Leishmania major in the presence of low amounts of interferon gamma ( IFN gamma ) . ^^^ In this report , evidence is provided that the synergism of IFN gamma and IL 4 is based on the ability of the lymphokines to induce the endogenous production of TNF alpha . ^^^ First , both IFN gamma and IL 4 as single agents and in combination were potent inducers of TNF alpha production by M phi infected with L . major amastigotes . ^^^ Second , the synergistic effect of IFN gamma and IL 4 on parasite killing by M phi strongly correlated with their synergistic effect on the release of TNF alpha . ^^^ Third , the IFN gamma / IL 4 mediated parasite elimination was completely abrogated not only in the presence of antibodies to IFN gamma and IL 4 , but also with an antibody specific for TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pulsing of cells with TNF alpha alone did not result in enhanced microbicidal activity but TNF alpha potentiated IFN gamma induced listericidal activity , resulting in high levels of killing when both cytokines were present . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Therefore , synthetic peptides corresponding to amino acid sequences 735 752 and 846 860 of glycoprotein gp 41 and to amino acid sequence 304 328 of gp 120 were investigated for their abilities to affect the production of the following cytokines by normal peripheral blood mononuclear cells in the presence of appropriate inducers : interferon ( IFN ) alpha , IFN gamma , interleukin ( IL ) 1 , IL 2 , and tumor necrosis factor ( TNF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of melanoma cells with recombinant IFN alpha or TNF substantially augmented the cytotoxicity of the immunoconjugate while treatment with IFN gamma had a minor effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Gamma interferon ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) significantly reduced the susceptibility to NK cell mediated lysis of transfected FO 1 cells . ^^^ Surprisingly , TNF alpha reduced the extent of lysis more than IFN gamma , although the latter cytokine enhanced HLA class 1 antigen expression more than the former one . ^^^ This finding , in conjunction with a reduction in the susceptibility to NK cell mediated lysis of untransfected FO 1 cells incubated with IFN gamma or TNF alpha , suggests that the two cytokines reduce NK cell mediated lysis of transfected cells by modulating not only the expression of HLA class 1 antigens , but also that of other structures . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A hyperdynamic sepsis model was set up in seven adult baboons to evaluate neutrophil activating peptide 1 / interleukin ( IL ) 8 ( NAP 1 / IL 8 ) , IL 1 beta , IL 6 , tumor necrosis factor alpha ( TNF alpha ) , and IFN gamma in plasma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Effects of granulocyte macrophage colony stimulating factor ( GM CSF ) , IL 2 , interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) and IL 6 on the production of immunoreactive IL 1 and TNF alpha by human monocytes . ^^^ The effects of GM CSF , IL 2 , IFN gamma , TNF alpha and IL 6 on the production of IL 1 ( both secreted and cell associated ) and TNF alpha by peripheral blood monocytes were studied . ^^^ Combination of GM CSF with IFN gamma , IL 2 or TNF alpha synergistically enhanced IL 1 secretion and had an additive effect on cell associated IL 1 production . ^^^ Combination of IL 2 with IFN gamma or TNF alpha also synergistically enhanced IL 1 secretion but the effect on cell associated IL 1 production was less than additive . ^^^ GM CSF synergistically enhanced TNF alpha secretion induced by IFN gamma but not by lipopolysaccharide . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , contrasting with the marked increase of TNF release by p 24 AM after their stimulation with IFN gamma ( p less than 0 . 001 ) , p24+ AM appeared to be refractory to any stimulation by IFN , arguing for their activation in vivo . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1 alpha , TNF alpha , and LPS increased ICAM 1 expression on average 100 200 % whereas IFN gamma was somewhat less potent . ^^^ Pretreatment with dexamethasone or transforming growth factor ( TGF ) beta did not affect baseline expression of ICAM 1 or inhibit upregulation of ICAM 1 on HDMEC by IL 1 alpha , TNF alpha , LPS , or IFN gamma . ^^^ Both IFN gamma and TNF alpha , but not IL 1 alpha increased MHC class 1 expression , whereas only IFN gamma induced the expression of HLA DR on HDMEC . ^^^ The effect of IL 1 alpha , TNF alpha , or IFN gamma was inhibited by antibody to the specific cytokine , but was unaffected by antibody to other cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Subsequently , Northern blot analyses performed on bulk LAK cells revealed a potent induction of mRNA for TNF alpha by 6 hrs and for IFN gamma by 48 hrs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumour necrosis factor ( TNF ) combined with gamma interferon ( IFN gamma ) is known to have antiproliferative effects on many tumour cells , both in vitro and in vivo . ^^^ Mesothelioma cell lines established from primary tumours did not respond significantly in vitro to either TNF or IFN gamma alone but were inhibited by combinations of TNF and IFN gamma at concentrations as low as 5 ng / ml . ^^^ In contrast , a mesothelioma cell line established from a metastatic tumour was sensitive to IFN gamma both alone and in combination with TNF but not to TNF alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The aims of the study were to determine whether and for how long IL 6 was detectable in the circulation of these patients , to assess whether IL 6 levels were associated with patients ' outcomes , and , finally , to examine the interplay between IL 6 , tumor necrosis factor ( TNF ) , interleukin 1 beta ( IL 1 beta ) , and interferon gamma ( IFN gamma ) . ^^^ The serum concentrations of IL 6 measured at study entry correlated with the duration of survival ( r = 0 . 51 , p = 0 . 004 ) and with the levels of TNF alpha ( r = 0 . 53 ; p less than 0 . 0001 ) but not with the levels of either IL 1 beta ( r = 0 . 01 , p = 0 . 90 ) or IFN gamma ( r = 0 . 06 , p = 0 . 60 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The present study was designed to examine the effect of physical exercise on production of interleukin 1 ( IL 1 ) , interleukin 6 ( IL 6 ) , tumour necrosis factor alpha ( TNF alpha ) , interleukin 2 ( IL 2 ) and interferon gamma ( IFN gamma ) . ^^^ TNF alpha , IL 2 and IFN gamma did not fluctuate in relation to exercise . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found that most clones from both the peripheral blood and CSF express IL 1 , IL 2 , IL 4 , IFN gamma , or TNF alpha cytokine mRNA after activation with ionomycin and PMA . ^^^ All T cell clones tested produced TNF alpha mRNA , and all but 14 produced IFN gamma mRNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To investigate the mechanism by which LPS down regulates its own induction of cytokine synthesis and the relationship between IL 6 and TNF production , we attempted to revert the inhibition of IL 6 and TNF production using agents like PMA or IFN gamma , previously reported to activate macrophage production of cytokines . ^^^ However , unlike PMA , IFN gamma could also partially restore TNF production in LPS tolerant mice , although only when LPS was administered at high doses . ^^^ By contrast with PMA , IFN gamma was clearly more active in restoring TNF synthesis than that of IL 6 . ^^^ These data suggest that different mechanisms are implicated in the inhibition of IL 6 and TNF synthesis in LPS tolerant mice and that part of this inhibition can be overcome by PMA or IFN gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha was compared with the cytokines IL 1 alpha and IFN gamma and with PMA and LPS . ^^^ IFN gamma did not induce endothelial cell detachment , but potentiated the effect of both TNF alpha and IL 1 alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
T cell mediated inhibition of erythropoiesis in aplastic anaemia : the possible role of IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cultured rat retinal astrocytes were tested by indirect immunofluorescence staining for their ability to express class 1 and 2 major histocompatibility complex ( MHC ) antigens under basal culture conditions and after three days of stimulation with two recombinant cytokines , rat interferon gamma ( IFN gamma ) and human tumour necrosis factor alpha ( TNF alpha ) . ^^^ IFN gamma and TNF alpha stimulation enhanced class 1 expression . ^^^ TNF alpha had no effect on class 2 expression , whereas IFN gamma induced the expression of class 2 in a dose dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Several other chemical reagents and a panel of recombinant cytokines including IL 2 , 3 , 4 , 5 , and 6 , granulocyte monocyte colony stimulating factor ( GM CSF ) , monocyte CSF ( M CSF ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) were tested for their induction of LIF secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TIL produced low levels of IL 2 , TNF and IFN gamma upon mitogen / phorbol ester stimulation , while PBL produce high levels of TNF and IFN gamma but low levels of IL 2 . ^^^ Under the same experimental conditions , LNL produce high levels of TNF and IL 2 but low levels of IFN gamma . ^^^ Mitogen mediated TNF secretion was increased after addition of autologous tumor cells in TIL and LNL , whereas IFN gamma secretion tended to be suppressed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The present communication examines the effect of exogenous hyperthermia on the detection of these cytokines and shows the suppressive effect of elevated temperature ( 39 degrees ) on the amount of IL 1 beta , IL 6 and IFN gamma ( P less than 0 . 001 ) but not on IL 1 alpha and TNF alpha concentrations . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Reduction in insulitis following administration of IFN gamma and TNF alpha in the NOD mouse . ^^^ In an effort to explore further the role of cytokines in the pathogenesis of IDDM , we examined clinical , metabolic and pathological features of NOD / Wehi mice injected intraperitoneally with multiple doses of IFN gamma and / or TNF alpha . ^^^ Compared with vehicle and cytokines alone , injection of IFN gamma + TNF alpha was associated with a variety of clinical and pathological changes including abdominal distention , piloerection , ascites , oedema , thymic atrophy , splenic enlargement and pancreatic distention . ^^^ The injection of IFN gamma + TNF alpha , and to a lesser extent TNF alpha alone , was associated with a significant reduction in the severity of insulitis . ^^^ Examination of pancreatic MHC class 1 and class 2 molecule expression revealed in mice given IFN gamma + TNF alpha , as compared with controls , significant and uniform induction of both these molecules on ductal and acinar cells ; low level MHC class 2 expression was also detectable on beta cells in these mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytotoxic effect of IFN gamma plus TNF alpha on human islet cells . ^^^ We have previously reported that the combination of IFN gamma plus TNF alpha is able to induce the de novo expression of HLA class 2 on human beta cells . ^^^ Three hour insulin release was markedly reduced in human islet monolayer cultures after 4 days ' exposure to 1000 U / ml of the combination TNF alpha plus IFN gamma ( 36 . 7 + / 7 . 7 , % of the control + / SEM ) or to TNF alpha alone ( 49 . 5 + / 7 % of the control ) while IFN gamma had little effect . ^^^ On direct inspection cell damage was clearly detected only in the cultures treated with TNF alpha plus IFN gamma in which staining by indirect immunofluorescence ( IFL ) for insulin revealed that the number of beta cells was also significantly reduced , thus suggesting a real cytotoxic effect of this cytokine combination . ^^^ This effect was not beta cell specific since glucagon release and the number of alpha cells were also reduced in the cultures exposed to IFN gamma plus TNF alpha . 51Cr release experiments supported the cytoxicity of these cytokines to normal islet cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
For IFN gamma and IL 2 , the effector activity can be suppressed by the presence of anti tumor necrosis factor alpha ( TNF alpha ) antibodies in the reaction mixture . ^^^ IFN gamma and IL 2 , only in combination , induce TNF alpha specific mRNA and secretion of this cytokine by macrophages . ^^^ When IFN gamma treated cells are infected with pathogens , they are stimulated to make TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Formation of interferon gamma ( IFN gamma ) and tumor necrosis factor ( TNF ) during Salmonella typhimurium infection was investigated in lipopolysaccharide ( LPS ) sensitive C3H / HeN and C57BL / 10ScSn ( B10ScSn ) , and LPS resistant ( lpsd mutant ) C3H / HeJ and C57BL / 10ScCr ( B10ScCr ) mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Positive reactions , i . e . , responses exceeding those on day 0 , were reached on day 10 with regard to the whole blood culture DNA synthesis response and IL 2 and IFN gamma secretion and on day 14 with regard to the mononuclear cell DNA synthesis response and tumor necrosis factor alpha ( TNF alpha ) secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The pretreatment of L 1210 or P 815 targets with IFN gamma before their exposure to LPS activated macrophages had no effect on the production of TNF . ^^^ Our results indicate that IFN gamma may act directly and differentially on tumor targets to alter their susceptibility for macrophage cytotoxicity , which was coupled to changes in the generation of cytotoxic nitric oxide , rather than TNF production by the macrophage . ( ABSTRACT TRUNCATED AT 400 WORDS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
They also produce cytokines known to inhibit hemopoiesis ( interferon gamma [ IFN gamma ] and tumor necrosis factor alpha [ TNF alpha ] ) or enhance it ( granulocyte macrophage colony stimulating factor , GM CSF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 2 therapy affected serum tumor necrosis factor ( TNF ) , interferon gamma ( IFN gamma ) and soluble IL 2 receptor ( sIL 2r ) levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Recombinant TNF alpha and IFN gamma at high concentrations are also toxic under the same test conditions , and TNF alpha was directly detected in LAK cell supernatants . ^^^ An inconsistent inhibition of toxicity was found with anti TNF alpha whereas anti IFN gamma antibodies had a partial inhibitory effect . ^^^ It is concluded that damage to vascular endothelium during high dose IL 2 treatments may be partially related to an excessive production of lymphokines such as IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Since Carbetimer could exert its antineoplastic action through immunomodulation , we also studied its effects on the production of TNF alpha and IFN gamma which are also known to affect bone metabolism . ^^^ Carbetimer did not stimulate TNF alpha release from isolated normal human monocytes or lymphocytes , but it markedly inhibited T lymphocyte production of IFN gamma , which became undetectable at a concentration of 1 mg of Carbetimer / ml . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our experiments show that murine peritoneal macrophages treated with IFN gamma in combination with tumor necrosis factor ( TNF ) or bacterial lipopolysaccharide ( LPS ) generate substantial amounts of citrulline as identified by amino acid analyzer and by thin layer chromatography . ^^^ In the absence of IFN gamma , TNF and LPS stimulate the conversion of arginine into ornithine but not citrulline . ^^^ However , when TNF or LPS stimulated macrophages are simultaneously treated with IFN gamma , ornithine production is relatively inhibited by the strong OAD reaction that competes with the ASE reaction for its substrate L arginine . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We examined the ability of LPS and several cytokines ( TNF alpha , IL 1 beta , IFN gamma , IL 4 ) to modulate IL 6 production by cultured human thymic epithelial cells ( TEC ) . ^^^ LPS as well as the cytokines TNF alpha and IL 1 beta was a potent inducer of IL 6 , increasing , respectively , IL 6 levels by 9 , 28 , and 75 fold ( mean values ) while IL 4 and IFN gamma provoked no significant effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Elevated levels of a number of cytokines such as Il 1 , IL 2 , IL 6 , interferon gamma ( IFN gamma ) , transforming growth factor beta and tumour necrosis factor alpha ( TNF alpha ) have been detected in the synovial fluid of patients with rheumatoid arthritis and other inflammatory arthritides . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To gain further insight into measles induced immune suppression we studied the production of soluble IL 2 receptor ( sIL 2R ) , interferon gamma ( IFN gamma ) , IL 1 beta , and tumor necrosis factor ( TNF alpha ) by peripheral blood mononuclear cells ( PBMC ) isolated from measles patients at various times after the onset of the rash . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We studied the correlation between interleukin 1 beta ( IL 1 beta ) , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) in cerebrospinal fluid ( CSF ) and clinical / laboratory findings in children with aseptic meningitis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Therefore , we performed clonal CFU GM assays , which more specifically reflect cytokine effects on CFU GM , using JCML peripheral blood mononuclear cells ( PBMNC ) and neutralizing antibodies against GM CSF , granulocyte colony stimulating factor ( G CSF ) , macrophage colony stimulating ( M CSF ) , interleukin 3 ( IL 3 ) , interleukin 1 alpha ( IL 1 alpha ) , interleukin 1 beta ( IL 1 beta ) , interleukin 4 ( IL 4 ) , interleukin 6 ( IL 6 ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We examined the antitumor effects of recombinant interferon gamma ( IFN gamma ) and tumor necrosis factor ( TNF ) on cervical adenocarcinoma cell lines , in vitro and in vivo . ^^^ All these observations suggest that IFN gamma or TNF can have positive effects in the treatment of patients with adenocarcinoma of the uterine cervix . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The present study demonstrates that TNF alpha regulates the expression of enhanced antimicrobial activity by triggering IFN gamma primed macrophages to kill or inhibit intracellular Toxoplasma gondii . ^^^ The ability of TNF alpha to trigger IFN gamma primed macrophages was blocked by rabbit anti TNF alpha polyclonal antisera but was not affected by polymyxin B indicating that TNF alpha triggering was not due to contamination with LPS . ^^^ Collectively , these findings demonstrate that TNF alpha performs an important regulatory role in the expression of enhanced anti microbial activity by IFN gamma primed macrophages . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The present work is a detailed study of the mechanism of IFN gamma and TNF alpha triggered IL 6 secretion and IL 6 gene expression in human monocytic THP 1 cells and of the effect of these cytokines on the expression of IL 6 surface receptor and IL 6R gene . ^^^ Although TNF alpha was shown to stimulate IL 6 expression in fibroblasts in monocytic THP 1 cells , IFN gamma is required for TNF to induce IL 6 expression . ^^^ The results reported here demonstrate that combined treatment of THP 1 cells with IFN gamma + TNF alpha induced IL 6 mRNA expression , whereas no significant induction was obtained by either cytokine alone . ^^^ Nuclear run on transcription assay showed that the increased level of IL 6 mRNA induced by IFN gamma + TNF alpha was associated with induction of gene transcription . ^^^ Sequential stimulation of THP 1 cells by IFN gamma and subsequently by TNF alpha did not allow IL 6 gene induction , suggesting that IFN gamma and TNF alpha induced or activated different signaling factors which should act together to trigger IL 6 gene transcription . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
As shown here , the capacity of phagocytosis to elicit NO 2 production by IFN gamma treated M phi was inhibited by antibody to murine recombinant tumor necrosis factor alpha ( rTNF alpha ) , suggesting that phagocytosis enabled IFN gamma to activate M phi via the induction of TNF alpha as an autocrine second signal . ^^^ Phagocytosis also increased M phi NO 2 production elicited by IFN gamma plus TNF alpha in L arginine deficient media . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This ELiSA was specific for hIL 5 , showing no crossreactivity to recombinant human GM CSF , IL 4 , TNF alpha , IFN gamma and mouse IL 5 ( mIL 5 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A single micro injection of Tumour Necrosis Factor alpha ( TNF ) or gamma Interferon ( IFN gamma ) into the lumbosacral spinal cord of the rat produced meningitis and mononuclear cuffs within the cord , an inflammatory response remarkably similar in pattern to that observed during experimental autoimmune encephalomyelitis ( EAE ) , a research analog of multiple sclerosis . ^^^ In other experiments , the accumulation of radiolabeled spleen cells into spinal cord was measured after the injection of various doses of TNF and IFN gamma , results indicated that both cytokines elicited accumulation of spleen cells in an additive but not synergistic manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We conducted a phase 1 study in which an intramuscular injection of interferon gamma ( IFN gamma ) at 10 , 50 , or 100 micrograms / m2 was followed 5 minutes later by an intramuscular injection of 10 , 50 , or 100 micrograms / m2 of tumor necrosis factor alfa ( TNF alpha ) at another site every other day for 20 days ( 10 doses ) . ^^^ The addition of TNF alpha to IFN gamma reduced both the magnitude and duration of IFN gamma mediated effects on peripheral blood monocyte expression of Fc receptors ( FcRs ) and HLA DR and production of hydrogen peroxide . ^^^ On the other hand , TNF alpha and IFN gamma appeared to have an additive stimulatory effect on the production of neopterin by monocytes . ^^^ The highest serum levels of neopterin were detected in patients who received the highest doses of both IFN gamma and TNF alpha . ^^^ If the activation of peripheral blood monocytes is the appropriate surrogate measure of the immune enhancement of the combination , then the simultaneous administration of IFN gamma and TNF alpha is ineffective , and future attempts to exploit the potential additive or synergistic effects of this combination of cytokines in humans may need to explore sequential administration schemata . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The IL 2 induced purging ability of BM contaminated with leukemic cells was increased by IL 1 , TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , synergistic effect of TNF with Interferon gamma ( IFN gamma ) was more evident in arterial ECs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of intraperitoneal injections of recombinant interleukin 1 alpha ( IL 1 ; 250 , 000 U / day ) , interleukin 2 ( IL 2 ; 50 , 000 units / day ) , interferon gamma ( IFN gamma ; 50 , 000 U / day ) and tumor necrosis factor alpha ( TNF ; 100 , 000 U / day ) , on the biodistribution of concanavalin A ( Con A ) activated , indium 111 labeled lymphocytes were evaluated in BALB / c mice . ^^^ TNF and IL 1 produced a significant increase in lung uptake of radiolabeled lymphocytes at 4 and 24 h , whereas IL 2 and IFN gamma decreased uptake at both time points . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The presence of different cytokines in the synovial fluid of patients with rheumatoid arthritis has been studied and the macrophage derived cytokines such as IL 1 , IL 6 , TNF alpha , TGF beta and PDGF have usually been detected in large quantities , whereas T cell produced cytokines ( IL 2 , IL 4 , IFN gamma ) are absent or present in small quantities . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Antitumor activity of TNF alpha , IL 1 , and IFN gamma against three human osteosarcoma cell lines . ^^^ The cell lines MG 63 , SAOS 2 , and TE 85 were incubated with TNF alpha , IL 1 , or IFN gamma alone or in combination . ^^^ TNF alpha , IL 1 , and IFN gamma had antiproliferative activity against all three cell lines . ^^^ TNF alpha and IFN gamma were the most effective against SAOS 2 ; MG 63 cells were the most sensitive to IL 1 , and TE 85 cells were resistant to TNF alpha and IL 1 but sensitive to IFN gamma . ^^^ The synergistic antitumor effect of TNF alpha plus IFN gamma , IL 1 alpha , or IL 1 beta or of IFN gamma plus IL 1 alpha or IL 1 beta was higher than that obtained when the cytokines were employed alone . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Of the other cytokines tested , TNF alpha and IFN gamma weakly induced IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
By using neutralizing antibody experiments , this activity was found to be attributed to interferon gamma ( IFN gamma ) , but not to tumor necrosis factor ( TNF ) , although both cytokines were produced from LNC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study we have measured IL 1 , IL 2 , IL 4 , IL 6 , interferon gamma ( IFN gamma ) and tumour necrosis factor ( TNF ) ( using both immunoassays and bioassays ) in sera from 50 patients affected by IDDM at the time of clinical diagnosis and 51 age and sex matched controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Procoagulant expression and TNF alpha production were not always co ordinately expressed ; interferon gamma ( IFN gamma ) synergized with LPS to enhance both responses but when IFN gamma was combined with Act D only procoagulant was upregulated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The available data suggest that study of additional lymphokines such as interferon gamma ( IFN gamma ) and tumor necrosis factor ( TNF ) may be useful in discriminating rejection from infections in transplant patients . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In subsequent experiments , the abilities of interferon beta ( IFN beta ) , tumor necrosis factor alpha ( TNF alpha ) , prostaglandin E 2 ( PGE 2 ) , and transforming growth factor beta ( TFG beta ) , alone or in combinations of 2 , to modulate tumoricidal activity triggered in BMM phagocytes by IFN gamma or CP , were compared . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , a high concentration of cardiolipin ( 40 micrograms / ml ) which is optimal for the induction of macrophage growth , completely suppressed LPS triggered TNF release from not only untreated macrophages but also IFN gamma primed macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The recruitment factors , FMLP , C fragment C5a , monocyte chemotactic protein 1 , or transforming growth factor beta 1 , were incapable of rescuing monocytes from PCD nor did they enhance PCD , whereas several inflammatory cytokines in addition to IL 1 beta and TNF alpha , including granulocyte monocyte CSF and IFN gamma , prevented monocyte PCD provided that sufficient levels of these cytokines were continuously maintained in the cultures . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
LPS ( or LPS and IFN gamma ) induced production of IL 1 , IL 6 , and TNF alpha proteins was significantly inhibited by IL 10 in two macrophage cell lines . ^^^ LPS or LPS and IFN gamma induced expression of IL 1 alpha , IL 6 , or TNF alpha mRNA was also inhibited by IL 10 as shown by semiquantitative polymerase chain reaction or Northern blot analysis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present paper , we demonstrate that GM CSF at a concentration of 50 U / ml ( 5 ng / ml ) promotes better cell survival and the differentiation of Mo into M phi displaying certain morphological differences as compared to control M phi such as an increased expression of Max 1 antigen , CR 3 and Fc gamma 2 receptors , higher phagocytic properties and increased capacities of cytotoxicity and TNF secretion when the cells are further activated by IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines tested were IL 1 through IL 6 , GM CSF , G CSF , CSF 1 , LIF , TGF beta , NGF , Epo or IFN gamma , LPS , PGE 2 , dexamethasone and cyclosporin A , also have no effect , either alone or in combination with TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore we demonstrate here that IL 10 , added to monocytes , activated by interferon gamma ( IFN gamma ) , LPS , or combinations of LPS and IFN gamma at the onset of the cultures , strongly inhibited the production of IL 1 alpha , IL 1 beta , IL 6 , IL 8 , TNF alpha , GM CSF , and G CSF at the transcriptional level . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukins ( IL ) 1 , 2 , 4 , 6 and soluble IL 2 receptor ( sIL 2R ) , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) were measured in CSF and serum from patients with relapsing remitting and chronic multiple sclerosis , Guillain Barr syndrome , chronic inflammatory demyelinating polyradiculoneuropathy , HIV infection , bacterial meningitis , viral encephalitis , meningeal carcinomatosis , hematologic meningeal malignancies , and disseminated melanoma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We measured circulating interferon gamma ( IFN gamma ) , tumor necrosis factor / cachectin ( TNF ) , and interleukin 6 ( IL 6 ) in nine children with FHL . ^^^ During active disease , elevated IFN gamma was detected in seven of seven children , TNF in six of six , and IL 6 in two of six children studied . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The pretreatment of mice with IL 6 , IL 8 , or IFN gamma had no protective effects on TNF alpha or IL 1 beta induced lethality in contrast to the protection observed by a pretreatment with TNF alpha / IL 1 beta themselves or with endotoxin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
For this purpose RINm5F cells were exposed in culture for 1 2 days to interleukin 1 beta ( IL 1 beta ) , interleukin 6 ( IL 6 ) , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) and interferon alpha ( IFN alpha ) at different concentrations . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OK 432 induced IFN gamma and TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The results showed that at the initiation of CAPD , there was a higher bactericidal activity , phagocytosis index , H2O2 production and interleukin 1 ( IL 1 ) , gamma interferon ( IFN gamma ) and tumor necrosis factor ( TNF ) production ability and MHC expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Many of transformed B cells tested appear to be refractory to the proliferative effects of a number of defined cytokines including IL 1 , IL 2 , IL 4 , IL 5 , IL 6 , IL 7 , TNF alpha , lymphotoxin and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma alone did not induce either cytokine , but in the presence of GM CSF it caused a synergistic ( 100 fold ) increase in TNF but not IL 1 production . ^^^ Macrophage colony stimulating factor ( M CSF ) alone or in combination with LPS , IFN gamma or IL 2 did not stimulate PBM to produce TNF or IL 1 in 24 hr culture . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF , interferon gamma and cell mediated cytotoxicity in anorexia nervosa ; effect of refeeding . `` Natural ' ' cell mediated cytotoxicity ( CMC ) , spontaneous release of tumor necrosis factor alpha ( TNF alpha ) by mononuclear cells in vitro and PHA induced interferon gamma ( IFN gamma ) production were examined by standard methods ( confirmed by neutralization with monoclonal antibodies ) in 14 female patients with marked weight loss due to anorexia nervosa ( AN ) and 16 matched healthy controls . ^^^ We found a significant increase in TNF production in AN , as well as a depressed CMC and IFN gamma production . ^^^ TNF , interferon gamma and cell mediated cytotoxicity in anorexia nervosa ; effect of refeeding . `` Natural ' ' cell mediated cytotoxicity ( CMC ) , spontaneous release of tumor necrosis factor alpha ( TNF alpha ) by mononuclear cells in vitro and PHA induced interferon gamma ( IFN gamma ) production were examined by standard methods ( confirmed by neutralization with monoclonal antibodies ) in 14 female patients with marked weight loss due to anorexia nervosa ( AN ) and 16 matched healthy controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although pretreatment with AcD , CsA , or ChT strongly inhibits production of IL 2 , TNF and IFN gamma , only clones pretreated with AcD lose cytolytic activity against Ag pulsed , Ia bearing LK cells . ^^^ Collectively , the data demonstrate that activated CD 4 cells express two distinct cytolytic activities ; a TNF ( and IFN gamma ) mediated cytotoxicity and a TNF ( and IFN gamma ) independent cytolytic activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
One type of CD4+ T cell with anti CD 3 mediated cytotoxic activity , in analogy with murine studies , is the inflammatory or TH 1 subtype , the main producer of interleukin ( IL 2 ) , interferon gamma ( IFN gamma ) and tumour necrosis factor ( TNF ) alpha , beta , whereas the other type of CD4+ T cell clone lacked anti CD 3 mediated cytotoxicity and produced minimal amounts of IL 2 concomitant with reduced levels of IFN gamma and TNF alpha , beta . ^^^ The present study demonstrates that among three MS patients , relatively more inflammatory CD4+ T cell clones with cytotoxic activity and IFN gamma and TNF alpha , beta production were derived from the cerebrospinal fluid as compared with peripheral blood lymphocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this paper we report that HIV 1 induces peripheral blood mononuclear cells ( PBMC ) and CD4+ T lymphocytes to secrete TNF alpha , TNF beta and interferon gamma ( IFN gamma ) , three cytokines having multifunctional activities and complex physiological roles . ^^^ Our study therefore demonstrates that efficient HIV replication in primary mononuclear cells is associated with the ability of the virus to induce TNF and IFN gamma secretion . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , preincubation of A 549 epithelial cell line monolayers with IL 4 caused no increase in T cell binding whereas A 549 cells and EC showed a similarly enhanced adhesiveness for T cells after preincubation with IL 1 , TNF , or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The CD4+45R T cell subset was an efficient producer of TNF beta and IFN gamma whereas the CD4+45R+ T cell subset produced significant amounts of TNF beta but only marginal amounts of IFN gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Bone remodeling is controlled by systemic factors such as parathyroid hormone ( PTH ) , calcitonin ( CT ) , and 1 , 25 ( OH ) 2 vitamin D and by local factors including cytokines and growth factors such as IL 1 , IL 2 , TNF alpha , TGF beta , IFN alpha , and IFN gamma . ^^^ Senile osteoporosis of low turnover thus appears to be associated with vitamin D deficiency , low macrophage function , high CD 4 lymphocyte proportion , low IL 1 and high IL 2 activity , low IFN alpha and high IFN gamma activity , and low TGF beta and TNF alpha activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Surface antigen expression of ICAM 1 and ICAM 1 specific mRNA could be significantly increased by incubating glioblastoma cells with interleukin 1 beta ( IL 1 beta ) , tumour necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) . ^^^ Significant enhancement of ICAM 1 expression was obtained using TNF alpha and IL 1 beta at 1 10 U / ml and at 500 U / ml of IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The weak , constitutive ICAM 1 expression on human keratinocytes ( KC ) can be up regulated by cytokines such as interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neutralization studies and direct determination characterized these cytokines as IFN gamma and TNF alpha . ^^^ Northern blot analysis demonstrated that activated B cells in co culture with NK clones were able to induce accumulation of mRNA transcripts for IFN gamma and TNF alpha in NK cells . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Phenotypically normal CD4+ and CD8+ T cells from both lpr and MRL / n also contained transcripts for IFN gamma , TNF alpha , TNF beta and IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although pretreatment with CsA or CT did not inhibit the cytolytic activity of CD 4 clones , lymphokine ( interleukin 2 , IL 2 , interferon gamma , IFN gamma , and tumor necrosis factor , TNF ) production was strongly inhibited . ^^^ The production of mRNA for TNF and IFN gamma by concanavalin A activated CD 4 clones was also inhibited by CsA and CT . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The intercellular adhesion molecule ( ICAM 1 ) has been shown to be important in interactions involving cells of the immune system and to be upregulated in a number of cell culture systems by cytokines , including immune interferon ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^ In the present study , we have determined by fluorescence activated cell sorter ( FACS ) analysis and the anti ICAM 1 monoclonal antibody ( MAb ) CL203 . 4 the base line expression of ICAM 1 and its modulation by recombinant IFN beta , IFN gamma and TNF alpha in early passage ( less than 15 ) human central nervous system ( CNS ) tumor derived cell cultures . ^^^ All the cell cultures displayed variable but consistent increases in ICAM 1 expression following treatment with IFN gamma or TNF alpha . ^^^ Upregulation of ICAM 1 expression in an established glioblastoma multiforme cell line was of greater magnitude and more rapid following TNF alpha treatment ( within 2 to 3 hr ) than exposure to IFN gamma ( by 24 hr ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The quantitative expression of ICAM 1 in various cell types can be either induced or enhanced by treatment with cytokines , such as interferon gamma ( IFN gamma ) , tumor necrosis factor ( TNF ) alpha or interleukin 1 ( IL 1 ) , a phenomenon which results in the augmentation of binding to LFA 1 positive cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The production of interleukin 2 ( IL 2 ) , gamma interferon ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) , lymphotoxin ( LT ) , interleukin 6 ( IL 6 ) and transforming growth factor beta ( TGF beta ) was assessed at the mRNA level by slot blot analysis in unstimulated clones as well as after activation with monoclonal anti CD 3 ( OKT 3 ) and IL 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both the sporozoites and the erythrocytic stages can modulate the hepatic phase by cytokines , notably IFN gamma , TNF and IL 6 , either directly or as a result of a cascade of events , and by MHC restricted and antibody dependent cell mediated cytotoxicity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We as well as others have noted increased levels of cytokines including IFN gamma , TNF , and IL 1 and the cell surface MHC class 2 and ICAM 1 molecules in the kidneys of mice with lupus nephritis . ^^^ Basal expression of ICAM 1 was upregulated rapidly by IFN gamma , TNF , and IL 1 . ^^^ MHC class 2 expression could not be induced with TNF or IL 1 alone but required prolonged stimulation with IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1 , IL 2 , TNF alpha , and IFN gamma were not able to stimulate C receptor mediated ingestion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The growth of cultured human keratinocytes ( KC ) is inhibited by gamma interferon ( IFN gamma ) , cyclosporin A and transforming growth factor beta , but not by tumor necrosis factor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
GMG cell RNA contained transcripts for LIF and CSF 1 but did not contain transcripts for GM CSF , G CSF , IL 2 , IL 3 , IL 4 , IL 6 , IL 7 , IFN gamma or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of infected macrophages with recombinant IFN gamma reduced intracellular proliferation of mycobacteria , while exogenous IL 2 and tumor necrosis factor were ineffective . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
FAC stimulates the production of TNF alpha and IFN gamma in human PBM , and this effect is potentiated by IL 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
T cell clones derived from cerebrospinal fluid ( CSF ) of patients with multiple sclerosis ( MS ) were analysed for their ability to produce interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) , interleukin 2 ( IL 2 ) and interleukin 4 ( IL 4 ) . ^^^ All the CSF T clones ( both CD4+ and CD8+ ) produced large amounts of IFN gamma and particularly of TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
At any stage of serum free adherent culture , monocyte to macrophage differentiation can be induced rapidly by the addition of serum , whereas cytokines ( rhM CSF , recombinant human granulocyte macrophage [ rhGM ] CSF , recombinant human granulocyte [ rhG ] CSF , recombinant human interleukin [ rhIL ] 1 , rhIL 3 , rhIL 4 , rhIL 6 , tumor necrosis factor [ TNF ] alpha , interferon [ IFN ] alpha , IFN gamma ) alone or in combination are not effective . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
B cells bind more strongly than T cells , an effect enhanced by lymphocyte activation or endothelial cell stimulation with interferon gamma ( IFN gamma ) or tumour necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
GM CSF was assayed by ELISA in supernatants from cultured RA fibroblast like synoviocytes stimulated with various cytokines ( IL 1 beta , TNF alpha , macrophage CSF , IFN gamma , IL 6 , and TGF beta ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study , we have investigated the ability of activated charcoals ( DHP 1 and Adsorba 150C ) and uncharged resin ( Amberlite XAD 7 ) to adsorb lipopolysaccharide ( LPS ) and various cytokines , namely tumour necrosis factor ( TNF ) , interleukin 1 ( IL 1 ) , interleukin 6 ( IL 6 ) , interferon alpha ( IFN alpha ) and interferon gamma ( IFN gamma ) . ^^^ This demonstrated that , TNF , IL 1 , IL 6 , IFN alpha and IFN gamma readily bind to proteins and / or other large molecules in plasma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serological and functional studies of affinity purified NZB SF have revealed that NZB SF is distinguishable from IL 1 alpha , IL 2 , IL 3 , IL 4 , IL 5 , IL 6 , CSF GM , IFN gamma , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro exposure of the tumor cells to IFN gamma and TNF alpha elevated class 1 antigen expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon alpha ( IFN alpha ) , interferon gamma ( IFN gamma ) , interleukin ( IL ) 1 , IL 2 , IL 3 , IL 4 , and tumor necrosis factor ( TNF ) alpha were added to these cultures and dose / kinetics of MHC class I / II induction quantitated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Results indicated that in the absence of OM 89 , production of the monokines interleukin 1 beta ( IL 1 beta ) and tumor necrosis factor alpha ( TNF alpha ) is increased , while that of the lymphokines interleukin 2 ( IL 2 ) and interferon gamma ( IFN gamma is decreased by phytohemagglutinin ( PHA ) stimulated PBMC from RA patients as compared with PBMC from healthy subjects . ^^^ In the presence of PHA , OM 89 enhanced the production of IL 1 beta , TNF alpha , IL 2 , and IFN gamma . ^^^ By contrast , in the presence of increasing amounts of OM 89 , TNF alpha secretion significantly higher and IFN gamma secretion significantly lower with PBMC from RA patients compared to PBMC from healthy subjects . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pretreatment of HUVEC with tumour necrosis factor ( TNF ) , IL 1 or interferon gamma ( IFN gamma ) led to increased binding . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Monocytes treated with LPS and lipopeptide ( in the absence of IFN gamma ) secreted IL 1 , TNF , and PGE 2 . ^^^ In contrast , monocytes incubated with MTP PE ( in the absence of IFN gamma ) secreted only TNF . ^^^ When the monocytes were coincubated with IFN gamma ( human but not mouse ) and the immunomodulators , IL 1 , TNF , and PGE 2 were secreted at all test groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
T cell clones derived from the cerebrospinal fluid of patients with multiple sclerosis were investigated for their ability to produce IL 2 , IL 4 , IFN gamma and TNF alpha . ^^^ All CSF clones ( both CD4+ and CD8+ ) produced high amounts of IFN gamma and particularly of TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 2 , IL 3 , IL 4 , IL 5 , IL 6 , IL 7 , GM CSF , TNF alpha , LT , IFN gamma and TGF beta did not have this effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) and beta ( TNF beta ) , and interferon alpha ( IFN alpha ) and gamma ( IFN gamma ) were detected in lesional extracts using immunoassays , however , no equivalent biological activities could be detected . ^^^ Tumor necrosis factor alpha ( TNF alpha ) and beta ( TNF beta ) , and interferon alpha ( IFN alpha ) and gamma ( IFN gamma ) were detected in lesional extracts using immunoassays , however , no equivalent biological activities could be detected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we tested inhibitors of arachidonate metabolism for possible protection against the toxic effects of the cytokine combination of tumor necrosis factor ( TNF , 100 U / ml ) and interferon gamma ( IFN gamma , 100 U / ml ) in rat islet cell monolayer cultures , using a 51Cr release cytotoxicity assay to measure islet cell lysis ( % 51Cr release ) . ^^^ The toxic effect of TNF / IFN gamma ( 26 . 6 + / 3 . 7 % ) was inhibited partially by both a cyclooxygenase inhibitor , indomethacin and a lipoxygenase inhibitor , nordihydroguaiaretic acid ( NDGA ) , and combination of maximally effective concentrations of Indo and NDGA ( 30 microM ) produced further protection against TNF / IFN gamma induced lysis ( 3 . 5 + / 0 . 9 % ) . ^^^ Also , the combined cyclo / lipoxygenase inhibitors , oxyphenbutazone and eicosa 5 , 8 , 11 , 14 tetrynoic acid , as well as the phospholipase A 2 inhibitor , bromophenacyl bromide , significantly inhibited the cytotoxic effect of TNF / IFN gamma . ^^^ Whereas indomethacin and NDGA did not prevent TNF / IFN gamma induced inhibition of insulin release , this recovered after cytokine removal from cultures protected by the cyclo / lipoxygenase inhibitors . ^^^ These results suggest that arachidonate metabolites may be involved in mediating the cytotoxic and not the functional inhibitory effects of TNF and IFN gamma in islet cells . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The dose of IFN gamma required for cytotoxicity is higher than that needed for antiviral and macrophage activation but can be reduced 10 to 100 fold by cotreatment of the cells with tumor necrosis factor or interleukin 1 ( IL 1 ) or both , two cytokines that by themselves are not toxic for these cells . ^^^ Inclusion of antibodies directed against tumor necrosis factor alpha or IL 1 alpha in the culture medium did not block the cytotoxic effect of combined IFN gamma plus LPS treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have studied the effect of IFN gamma and of 1 , 25 ( OH ) 2D3 on the expression of IL 1 and TNF alpha messenger RNA in response to LPS . ^^^ This striking effect of IFN gamma on the kinetics of IL 1 gene response does not apply to the TNF alpha gene . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The inflammatory cytokines IL 1 , and , to a lesser extent , TNF alpha and IFN gamma increased IL 6 production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The otherwise modest ability of CTL supernatants to stimulate the proliferation of fresh bone marrow cells was augmented considerably in the presence of anti IFN gamma mAb , and this activity was appropriately blocked by anti IL 3 and anti GM CSF antibodies . mRNA for IL 3 and GM CSF , as well as for IFN gamma and TNF alpha , was detected in cells that secreted those lymphokines , and the time course of appearance of each mRNA correlated with secretion of the appropriate lymphokine activity . ^^^ However , the time course of mRNA accumulation for each lymphokine was distinct , the order of expression of these genes apparently being TNF alpha , then IFN gamma and GM CSF , and finally IL 3 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To determine the role of keratinocytes in both the chemotaxis and adhesion of these cells to the epidermis , cultured keratinocytes were incubated with IFN gamma and tumor necrosis factor alpha ( TNF alpha ) , and mRNA detected and quantitated for IL 8 , monocyte chemotaxis and activating factor , and intercellular adhesion molecule 1 . ^^^ Whereas induction of these mRNAs was either absent , or relatively weak and transient , to either IFN gamma or TNF alpha alone , when administered in combination there was a dramatic increase and persistence in the induction of all three genes . ^^^ These novel findings potentially explain the selective recruitment of T cells and monocytes observed in inflammatory skin disease , because IFN gamma and TNF alpha can co ordinately regulate keratinocyte derived chemoattractants and adhesion molecule production . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cultured neonatal rat ( F 344 , RT 1 ( 1v1 ) ) islets that were devoid of MHC class 2 ( OX 6 ) antigen and antigen presenting cells were treated with recombinant murine interferon ( IFN gamma ) and / or recombinant murine tumor necrosis factor ( TNF alpha ) in vitro . ^^^ The IFN gamma and TNF alpha resulted in some disruption of the integrity of the islets by 7 days of culture , but the combination resulted in disaggregation of the islets within 7 8 days . ^^^ This effect of IFN gamma was potentiated by TNF alpha resulting in 27 % of the cells expressing class 2 antigen . ^^^ Islets treated with IFN gamma and TNF alpha alone or in combination were not rejected in a subsequent transplant underneath the kidney capsule of WF rats ( RT1u ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although IFN gamma , IL 6 , granulocyte CSF , and granulocyte macrophage CSF induced nitroblue tetrazolium reducing activity of U 937 cells , only IFN gamma , and TNF induced it synergistically in combination with TGF beta 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Purified normodense eosinophils were incubated with cytokines ( including rIL 1 , rIL 2 , rIL 3 , rIL 4 , rIL 5 , rIL 6 , IFN gamma , granulocyte macrophage CSF stimulating factor ( GM CSF ) , and TNF ) for 1 to 3 h after which Ig coupled Sepharose 4B beads were added as targets and the mixtures were incubated with the eosinophils at 37 degrees C for 4 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We determined whether BC and leukemia colony forming cells ( L CFC ) from AML patients could differentiate in vitro after short term culture with interferon gamma ( IFN gamma ) , 1 , 25 dihydroxyvitamin D 3 ( D 3 ) , retinoic acid ( RA ) , tumor necrosis factor alpha ( TNF alpha ) , and granulocyte monocyte colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have examined the effects of in vitro aging on the growth capacity of human umbilical vein endothelial cells ( HUVECs ) under the influence of tumor necrosis factor ( TNF ) with or without interferon gamma ( IFN gamma ) . ^^^ The effect of TNF was augmented by IFN gamma at a dose that alone affected growth and colony formation only slightly . ^^^ The augmentation by IFN gamma was also found to depend on in vitro age ; the synergy with TNF in the deterioration of colony forming ability was observed only in `` aged ' ' cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytotoxic factor was compared with purified human recombinant cytotoxic cytokines , including tumor necrosis factor ( TNF ) , lymphotoxin ( LT ) , interferon ( IFN ) alpha , IFN gamma , interleukin 1 alpha ( IL 1 alpha ) and IL 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , both TNF and LT substantially enhanced IFN gamma induced expression of HLA DQ while decreasing that of HLA DP . ^^^ Also , in dermal fibroblasts , IFN gamma induced HLA DP expression was significantly inhibited in the presence of TNF . ^^^ These data demonstrate that TNF and LT differentially modulate IFN gamma induced MHC antigen expression in mesenchymal cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cooperative effect of TNF alpha and IFN gamma was only seen when these recombinant cytokines were injected together at the proper time before the challenge . ^^^ Injection of rabbit anti TNF alpha serum abolished the effects of TNF alpha and the cooperative effect of TNF alpha and IFN gamma . ^^^ Furthermore , the serum could abolish the cooperative effect of IFN gamma and LPS on bactericidal activity , suggesting participation of LPS induced TNF alpha in the cooperation . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , although the growth stimulating activities of both substances were inhibited by IFN gamma and TNF , CL was more resistant to these inhibitory effects . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Thyroid cell survival was assessed in terms of mitochondrial dehydrogenase activity in monolayers exposed to tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , interleukin 1 ( IL 1 alpha and beta ) and interleukin 2 ( IL 2 ) in the presence or absence of thyroid stimulating hormone ( TSH ) . ^^^ Neither TNF alpha nor IL 2 affected thyroid cell survival , IFN gamma was usually inhibitory and IL 1 alpha slightly enhanced cell survival in some experiments . ^^^ In contrast , IFN gamma , IL 2 and TNF alpha inhibited the ability of thyroid lymphocytes from patients with Graves ' disease and Hashimoto ' s thyroiditis to synthesize autoantibodies to thyroid peroxidase ( TPO ) and thyroglobulin ( Tg ) . ^^^ Our studies suggest that in addition to its well recognized ability to induce MHC class 2 antigens on thyroid cells , IFN gamma may inhibit thyroid cell proliferation and TNF alpha , IFN gamma and IL 2 may down regulate thyroid autoantibody synthesis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here we report that bis [ 2 ( N phenyl carboxamido ) ] phenyl diselenide , 2 phenyl 1 , 2 benzisoselenazol 3 ( 2H ) one ( Ebselen ) and related compounds are inducers of interferon gamma ( IFN gamma ) and tumor necrosis factor ( TNF ) in human peripheral blood leukocytes . ^^^ The IFN gamma and TNF production was reduced after removing adherent cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Differential effects of LPS , IFN gamma and TNF alpha on the secretion of lysozyme by individual human mononuclear phagocytes : relationship to cell maturity . ^^^ Human mononuclear phagocytes can be activated to perform a variety of complex functions by exposure to the immunomodulators , lipopolysaccharide ( LPS ) , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) . ^^^ This enabled the direct effects of LPS , IFN gamma and TNF alpha on the secretion of LZM by individual , immunoidentified human mononuclear phagocytes to be investigated . ^^^ The overall secretion of this peptide by populations of freshly isolated or 3 day cultured monocytes was augmented by exposure for 6 hr to bacterial LPS , recombinant human IFN gamma or recombinant human TNF alpha . ^^^ Extension of the culture period for monocytes from 3 to 7 days prior to use in the assay resulted in higher levels of LZM secretion , which could be further increased by TNF alpha but not by LPS or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin 1 alpha and beta ( IL 1 alpha and beta ) , tumour necrosis factors alpha and beta ( TNF alpha and beta ) and interferon gamma ( IFN gamma ) were tested for their chemotactic effects , their effects on chemotactic movement towards N formyl methionyl leucyl phenylalanine ( FMLP ) and their effects on random locomotion of human peripheral blood neutrophils through polycarbonate membranes in Boyden type chambers . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This suppression was not mediated by TNF alpha / beta or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effect of LPS on constitutive and IFN gamma regulated C 4 synthesis is probably not mediated via release of endogenous monokines IL 1 beta , TNF alpha , or IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Injection of IFN gamma , IFN alpha / beta , and TNF alpha into the skin of rats stimulated the migration of small peritoneal exudate lymphocytes ( sPEL ) into the injection site , and these cytokines mediated lymphocyte recruitment to delayed type hypersensitivity , sites of virus injection , and in part to LPS . ^^^ IFN gamma , IFN alpha / beta , IL 1 , TNF alpha , and TNF beta increased the binding of small peritoneal exudate lymphocyte ( sPEL ) to EC . ^^^ Combinations of IFN gamma and TNF alpha were additive in stimulating sPEL EC adhesion . ^^^ Depletion of sPEL and spleen T cells by adherence to IFN gamma stimulated EC decreased the in vivo migration of the lymphocytes to skin sites injected with IFN gamma , IFN alpha / beta , TNF alpha , poly 1 : C , LPS , and to delayed type hypersensitivity reactions by 50 % , and significantly increased the migration of these cells to normal lymph nodes , as compared to unfractionated lymphocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro studies showed that interferon gamma ( IFN gamma ) increased the antimicrobial activity of murine peritoneal macrophages whereas TNF , IL 1 alpha and IL 1 beta did not . ^^^ TNF , however , synergized with the anti toxoplasmic effect provided by IFN gamma and this activity was blocked by anti TNF antibodies . ^^^ IFN gamma induced the production of TNF and the anti toxoplasmic effect provided by IFN gamma seemed to be dependent partly on the production of TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
LPS induced mRNA for IL 1 beta was modestly enhanced by IFN gamma whereas mRNA levels for TNF were markedly increased . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of recombinant tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) on B 16 mouse melanoma experimental metastatic ability and major histocompatibility complex ( H 2b ) antigens expression were studied . ^^^ The simultaneous treatment with TNF alpha and IFN gamma amplified the enhancement of experimental metastasis and all other effects obtained with TNF alpha alone . ^^^ The B 16 clone B78H1 , selectively resistant to H 2 induction and to enhancement of metastatic ability by IFN gamma , was not affected by treatment with TNF alpha and with TNF alpha + IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Gamma interferon ( IFN gamma ) , interleukin 1 ( IL 1 ) , and tumor necrosis factor ( TNF ) all significantly enhanced the anti Candida activity of GG2EE cells . ^^^ The augmentation of anti Candida activity was not always concomitant with enhancement of phagocytosis , since IFN gamma and colony stimulating factor 1 , but not IL 1 or TNF , augmented the phagocytic ability of GG2EE cells . ^^^ These findings demonstrate that GG2EE cells exhibit spontaneous anti Candida activity and that such activity is enhanced by TNF , IL 1 , and IFN gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 2 alone did not induce the expression of either gene while IFN gamma or IFN beta had a modest inductive effect on IP 10 but no effect on TNF alpha expression . ^^^ IFN gamma and IL 2 could synergize to stimulate the expression of either TNF alpha or IP 10 mRNA using sequential non overlapping treatment periods regardless of the order in which the two stimuli were applied to the cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we have examined the capacity of astrocytes to secrete TNF alpha in response to a variety of biologic stimuli , particularly cytokines such as IL 1 and IFN gamma , which are known to be present in the central nervous system during neurologic diseases associated with inflammation . ^^^ Rat astrocytes do not constitutively produce TNF alpha , but have the ability to secrete TNF alpha in response to LPS , and can be primed by IFN gamma to respond to a suboptimal dose of LPS . ^^^ IFN gamma and IL 1 beta alone do not induce TNF alpha production , however , the combined treatment of IFN gamma and IL 1 beta results in a striking synergistic effect on astrocyte TNF alpha production . ^^^ Astrocyte TNF alpha protein production induced by a combined treatment of either IFN gamma / LPS or IFN gamma / IL 1 beta occurs in a dose and time dependent manner , and appears to require a `` priming signal ' ' initiated by IFN gamma , which then renders the astrocyte responsive to either a suboptimal dose of LPS or IL 1 beta . ^^^ Astrocyte TNF alpha production by IFN gamma / LPS stimulation can be inhibited by the addition of anti rat IFN gamma antibody , whereas IFN gamma / IL 1 induced TNF alpha production is inhibited by antibody to either IFN gamma or IL 1 beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum concentrations of immunoreactive tumor necrosis factor / cachectin ( TNF ) , interleukin 1 beta ( IL 1 beta ) , interferon gamma ( IFN gamma ) , and interferon alpha ( IFN alpha ) were prospectively measured in 70 patients with septic shock to determine their evolution and prognostic values . ^^^ In a univariate analysis , levels of TNF ( P = . 002 ) and IL 1 beta ( P = . 05 ) were associated with the patient ' s outcome , but not IFN alpha ( P = . 15 ) and IFN gamma ( P = . 26 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this report , we have demonstrated that IFN gamma and TNF alpha increase expression of both the 1 A and 1 E region gene products on the surface of the myelomonocytic cell line WEHI 3 , and that they mediate this increase via an increase in A alpha transcription . ^^^ Constructs containing 5 ' deletion mutations of the A alpha promoter attached to the bacterial chloramphenicol acetyl transferase gene were used to delineate the minimum 5 ' flanking sequences required for promoter activity , and for inducibility by IFN gamma and TNF alpha . ^^^ Approximately 115 bp of 5 ' sequences are required for minimum induction by IFN gamma or TNF alpha when the cytokines are present separately . ^^^ Nested linker scanner mutations demonstrated that additional regions were also critical for optimal induction by IFN gamma or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These findings indicate that human SaOS 2 cells , fulfilling several criteria for osteoblasts , respond to TNF alpha and IFN gamma , resulting in decceleration of their maturation . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Peak serum TNF and IFN gamma levels were reached , respectively , at 1 and 4 hr following the first OKT 3 injection . ^^^ Thus , the highest TNF , IFN gamma , and IL 2 serum levels were detected in patients who did not receive corticosteroids . ^^^ Patients who received high dose corticosteroids ( 1 g solumedrol bolus ) concomitantly with the first OKT 3 injection still had high TNF and IFN gamma levels . ^^^ Conversely , when the same corticosteroid dose was injected 15 60 min prior to the first OKT 3 injection , in all cases the increase of serum TNF and IFN gamma was significantly lower as compared with the above described groups ; IL 2 levels did not rise . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma also diminished the stimulatory effects of both IL 1 and TNF . ^^^ In contrast , in 1 % serum IL 1 and TNF individually had relatively minor effects , while IFN gamma inhibited collagen production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The recombinant cytokines IL 1 , IL 2 , IFN alpha , IFN gamma and TNF also enhanced beta 2m production rates of both normal and leukaemic B cells , but to a considerably lesser extent than did TPA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We employed a tumor type specific tissue culture model utilizing three human colon carcinoma cell lines to ( 1 ) assess the effects of schedule , sequence , dose , and duration of exposure on the antiproliferative activity of combinations of tumor necrosis factor ( TNF ) and interferon gamma ( IFN gamma ) and to ( 2 ) determine the effects of this combination on the production of a TNF inducible protein , IFN beta 2 , and an IFN inducible protein , p 78 . ^^^ With minor exceptions , the maximal antiproliferative effect in all three cell lines was observed when IFN gamma and TNF were administered simultaneously , regardless of the doses of each agent and duration of exposure . ^^^ Exposing the cells to TNF before IFN gamma resulted in the least inhibition in cell growth . ^^^ No p 78 induction was observed in the HCT 116 cell line with IFN gamma alone , TNF alone , or the combination ; p 78 was slightly induced in the SKC 01 and VACO 9P cell lines with IFN gamma alone , and was synergistically induced by the combination of TNF and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When anti IFN gamma and anti TNF alpha antibodies were added simultaneously , full recovery of colony formation was observed . ^^^ In the CM of IL 2 treated lymphocytes , detectable levels of IFN gamma ( 81 + / 15 U / ml ) and TNF ( 3 . 1 + / 1 . 1 U / ml ) were found . ^^^ Addition of IFN gamma and TNF alpha at these concentrations to the agar culture inhibited CFU GM colony formation . ^^^ Taken together , these results indicate that inhibition of human CFU GM colony formation by IL 2 treated lymphocytes and their CM is mediated by IFN gamma and TNF alpha generated from IL 2 treated lymphocytes . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We used a modified 51Cr release cytotoxicity assay to define the interactive cytotoxic effects of interleukin 1 ( IL 1 ) , tumor necrosis factor ( TNF ) , and interferon gamma ( IFN gamma ) on rat islet cells in monolayer culture . ^^^ We here demonstrate that ( a ) IFN gamma , but not IL 1 or TNF , can sensitize islet cells to subsequent cytolysis by other cytokines ( IL 1 or TNF ) ; ( b ) the cytolytic effect of sequential addition of IFN gamma , then IL 1 or TNF ( approximately 35 40 % 51Cr release ) is similar to that produced by the concurrent addition of IFN gamma plus IL 1 or TNF ; ( c ) the priming effect of IFN gamma persists for 3 to 6 or more days after its removal ; and ( d ) islet cells preincubated with IFN gamma are also more sensitive to cytolysis by splenic mononuclear cells from diabetes prone BB / Wor rats . ^^^ These findings suggest that IFN gamma produced by activated T lymphocytes and monocytic cells infiltrating islets in Type 1 diabetes may play a direct and important role in sensitizing beta cells to damage by other cytokines ( IL 1 , TNF ) and cytotoxic cells in the immune / inflammatory infiltrate . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study we tested the ability of various cytokines , including IL 1 , TNF , IFN alpha , and IFN gamma to induce ADCC in vivo . ^^^ We found that both IFN alpha and IFN gamma induced ADCC in the livers and spleens of C3H / Hen treated mice and that these cytokines together with TNF enhanced the IL 2 induced ADCC in vivo . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Intraperitoneal treatment with recombinant murine ( rMu ) IFN gamma ( greater than or equal to 10 , 000 units / mouse ) or recombinant murine TNF alpha ( greater than or equal to 10 , 000 units / mouse ) produced greater than 60 % inhibition of metastasis formation . ^^^ Neither IFN gamma nor TNF alpha inhibited colony formation of M 109 cells in vitro , suggesting a host mediated mechanism for antitumor activity . ^^^ Peritoneal macrophages were primed for tumor cytotoxicity by treatment with either IFN gamma or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Normal donor PBLs showed a transient release of tumor necrosis factor ( TNF ) ( up to 400 pg / ml ) during the first day , while factors including interferon gamma ( IFN gamma ) , soluble IL 2 receptor , and soluble CD 8 showed a gradual increase to modest levels ( at best ) during the 4 day incubation with IL 2 . ^^^ In pattern 2 , the patients ' PBLs from each cytapheresis released only low levels of TNF ( less than 300 pg / ml ) and minimal levels of IFN gamma , IL 2 receptor , and CD 8 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our findings document the presence of cytokine receptors in human pancreatic carcinoma cells and suggest that postreceptor events rather than differences in receptor number or affinity more likely govern the responsiveness of pancreatic cancer cells to TNF and IFN gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor ( TNF ) synergistically enhanced the antiproliferative activity of interferon gamma ( IFN gamma ) in both TNF sensitive and TNF resistant variants of the cervical carcinoma line , ME 180 . ^^^ When used in combination in both cell lines , TNF and IFN gamma induced the synthesis of 10 polypeptides that were not induced by either agent alone . ^^^ These synergistically induced polypeptides may be crucial to the mechanism of the synergistic antiproliferative action of TNF and IFN gamma in ME 180 cells . . ^^^ Tumor necrosis factor ( TNF ) synergistically enhanced the antiproliferative activity of interferon gamma ( IFN gamma ) in both TNF sensitive and TNF resistant variants of the cervical carcinoma line , ME 180 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To elucidate the mechanism of decreased 131I uptake by the thyroid gland in patients with subacute thyroiditis and painless thyroiditis , human thyroid follicles were cultured with interleukin 1 ( IL 1 ) , tumor necrosis factor alpha ( TNF alpha ) , and / or interferon gamma ( IFN gamma ) , and the effects of these cytokines on thyroid function were studied in vitro . ^^^ TNF alpha and IFN gamma also inhibited thyroid function in a concentration dependent manner . ^^^ Furthermore , when thyrocytes were cultured with IL 1 , TNF alpha and IFN gamma , these cytokines more than additively inhibited thyroid function . ^^^ Although the main mechanism of 131I uptake suppression in the thyroid gland in subacute thyroiditis is due to cellular damage and suppression of TSH release , our present findings suggest that IL 1 , TNF alpha , and IFN gamma produced in the inflammatory process within the thyroid gland further inhibit iodine incorporation and at least partly account for the decreased 131I uptake by the thyroid gland in destruction induced hyperthyroidism . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Coincubation with interferon gamma ( IFN gamma ) did not change the amount of IL 1 induced TNF alpha , whereas in the same culture IFN gamma inhibited ( greater than 70 % ) IL 1 induced IL 1 production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The sheep anti TNF alpha antibody preparation was specific for TNF since it neutralized 300 U of recombinant murine TNF alpha in vitro at a dilution of up to 1 / 1 , 000 but did not neutralize 32 U of interferon ( IFN ) alpha , beta or 32 U of IFN gamma in vitro at a 1 / 20 dilution . ^^^ From the data presented we conclude that in addition to other lymphokines , such as IFN gamma , TNF alpha is of importance during the entire course of a Listeria infection in nu / nu mice . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines produced by mononuclear cells are important regulatory and effector molecules and evidence has been presented to support a role at least for tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) in host defense against Leishmania . ^^^ In the present study , we examined the production of TNF alpha and interleukin 1 ( IL 1 ) by resting and IFN gamma primed peripheral blood monocytes infected in vitro with Leishmania donovani . ^^^ Pretreatment of cells with IFN gamma abrogated infection induced inhibition of IL 1 production and primed cells for the production of both IL 1 and TNF alpha upon subsequent exposure to Leishmania . ^^^ The ability of IFN gamma to prime monocytes to produce TNF alpha and IL 1 in response to infection with Leishmania and to prevent inhibition of IL 1 production may have implications for immunotherapy with this lymphokine . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Also , in mice that were protected by anti IFN gamma antibody , serum IFN and TNF were undetectable , whereas IL 6 levels were as high as in unprotected mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In KD patients with coronary artery lesions ( CAL ) , the percentage of positive cases for TNF ( greater than or equal to 10 units / ml ) , IL 2R ( greater than or equal to 1056 units / ml ) and IFN gamma ( greater than or equal to 0 . 3 units / ml ) was higher than that in patients without CAL . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
At pH 4 . 0 or 4 . 5 , where these three cytokines were bound almost completely to the liposome membranes , ( 1 ) the ratios of the rates of membrane action of TNF and IFN alpha on PS containing liposomes to those on PG or PA containing liposomes were similar , but always greater than the ratio of the former to the latter rates of IFN gamma , and ( 2 ) progressive replacement of either PG or PA in membranes by PS at a constant anionic phospholipid concentration decreased or increased the rates of membrane action of TNF and IFN alpha but only decreased that of IFN gamma , suggesting that membrane PS modulates the membrane actions of these three cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha and IFN gamma reverse IL 4 inhibition of lymphokine activated killer cell function . ^^^ Addition of IFN gamma or TNF alpha partially reverses IL 4 mediated inhibition ( 30 100 % ) in a dose dependent fashion . ^^^ IFN gamma and TNF alpha must be added within the first 24 hr of initiating culture in order to reverse IL 4 inhibition . ^^^ Furthermore , IFN gamma and TNF alpha are most effective at reversing IL 4 inhibition at low concentrations of IL 2 ( less than 100 u / ml ) . ^^^ In addition to suppression of LAK induction , IL 4 also inhibits IL 2 induced IFN gamma and TNF alpha protein production in PBMC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The murine macrophage tumor lines , P388D1 and J774A . 1 were stimulated with LPS , IL 6 , IL 1 alpha , TNF alpha , IFN gamma , PMA , or combinations of these reagents to induce the production of IL 6 , IL 1 , and TNF alpha . ^^^ IFN gamma and PMA synergize with LPS in the production of IL 6 as well as IL 1 and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated 45 patients with Kawasaki disease ( KD ) and report the first simultaneous determination of tumor necrosis factor ( TNF ) , interleukin 2 receptor ( IL 2R ) and interferon gamma ( IFN gamma ) in the serum during acute phase . ^^^ Serum levels of TNF , IL 2R , and IFN gamma were seen to increase during the acute phase of KD . ^^^ In KD patients with coronary artery lesions ( CAL ) , the percentage of positive cases for TNF ( greater than or equal to 10 U / ml ) , IL 2R ( greater than or equal to 1056 U / ml ) , and IFN gamma ( greater than or equal to 0 . 3 U / ml ) was higher than that in patients without CAL . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The mean amounts of production of tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 beta ( IL 1 beta ) , and interferon gamma ( IFN gamma ) by PBMCs from patients with PBC tended to be increased in cultures in the presence of stimulating agents in comparison with controls , but there was no significant difference because of a wide scatter of results . ^^^ The number of TNF alpha or IFN gamma positive infiltrating mononuclear cells detected by immunohistochemical staining in liver biopsy sections correlated with the production of these cytokines by PBMCs in vitro . ^^^ In patients with type B chronic active hepatitis , IL 1 beta and IFN gamma production was similar to controls , while TNF alpha production tended to be enhanced . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the absence of interferon gamma ( IFN gamma ) or lipopolysaccharide , TNF alpha ( 0 . 025 25 , 000 U / ml ) failed to activate peritoneal exudate M phi from BALB / c mice for killing of L . major amastigotes . ^^^ In the presence of suboptimal doses of IFN gamma ( 5 or 10 U / ml ) , however , TNF alpha mediated a rapid elimination of intracellular parasites , which was highly significant compared to IFN gamma alone . ^^^ From these data we conclude that the presence of IFN gamma is crucial for TNF alpha mediated killing of L . major parasites by M phi . ^^^ Disease progression in susceptible mice therefore seems to be a consequence of a deficiency of IFN gamma and a predominance of interleukin 4 rather than the result of an excess amount of TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF and IFN gamma are thought to be involved in the immune response to mycobacterial infection because they exhibit antimycobacterial effects in vitro . ^^^ Both TNF and IFN gamma were selectively concentrated 5 to 30 fold in pleural fluid , compared to blood of the same patients . ^^^ In contrast to results obtained for TNF release , the protein peptidoglycan complex , but not lipoarabinomannan , stimulated IFN gamma release by pleural fluid mononuclear cells . ^^^ Local production of tumor necrosis factor and IFN gamma in tuberculous pleuritis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To identify potential effects on two types of blastocyst derived cells , TNF alpha , IFN alpha / beta , and IFN gamma were tested for the ability to modulate proliferation and the expression of class 1 MHC Ag by rat trophoblast cells and embryonic fibroblasts . ^^^ Growth of the trophoblast cells was inhibited by TNF alpha , IFN alpha / beta , and IFN gamma , whereas both TNF alpha and IFN alpha / beta enhanced fibroblast proliferation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The combination of GM CSF ( 10 ( 2 ) U / ml ) plus TNF ( 10 ( 2 ) U / ml ) augmented macrophage killing ( range , 31 + / 4 % ) compared with GM CSF ( 10 ( 2 ) U / ml ) alone , but the combination of recombinant human interferon gamma ( IFN gamma ) plus GM CSF resulted in a significant decrease in intracellular inhibition of growth or killing ( 13 . 3 + / 2 % ) compared with 57 . 7 + / 5 % obtained with GM CSF alone . ^^^ These results indicate that : 1 ) GM CSF can activate macrophages to inhibit intracellular growth or kill MAC ; 2 ) killing may be augmented by TNF ; and 3 ) IFN gamma may impair GM CSF dependent macrophage activation . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The intensity of MHC class 2 antigen expression on bronchial epithelium and vascular endothelium in graft lungs increased with the progression of rejection response and directly correlated with the bronchoalveolar lavage fluid ( BALF ) levels of biochemical markers , as tumor necrosis factor alpha , gamma interferon ( IFN gamma ) , interleukin 2 ( IL 2 ) and soluble interleukin 2 receptor ( SIL 2R ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , TNF alpha INH affected TNF alpha synergism with IFN gamma induced HLA DR antigen expression but had no effect on IFN gamma activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This reduction of specific mRNA is also overcome by addition of IFN gamma , but not by IFN alpha and IFN beta , indicating that pretranslational mechanisms are responsible for the regulation of TNF in desensitization . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have previously utilized a bank of diverse human colorectal carcinoma cell lines to assess the synergistic antiproliferative effect of tumor necrosis factor ( TNF ) and IFN gamma ( IFN gamma ) in combination . ^^^ In this study , we used 3 of these cell lines ( HCT 116 , SKO 1 and VACO 9P ) to study the growth inhibitory effects of TNF and IFN gamma ( TNF / IFN gamma ) on these cells when administered in conjunction with 5 fluorouracil ( 5 FU ) . ^^^ All 3 cell lines were sensitive to the anti proliferative effects of TNF / IFN gamma in a dose dependent and duration dependent fashion . ^^^ TNF / IFN gamma was administered for 1 hr every other day on days 1 , 3 and 5 to the 3 cell lines . ^^^ The addition of TNF / IFN gamma to clinically achievable concentrations of 5 FU in both schedules resulted in additive cytotoxicity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Exposure of rabbit chondrocytes to either IFN gamma or TNF primed the chondrocytes to produce significantly greater amounts of hydrogen peroxide with or without further stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The levels of TNF alpha and IFN gamma were examined in serum from 32 patients with multiple myeloma and 33 healthy controls using sensitive enzyme linked immunosorbent assays ( ELISA ) . ^^^ The detection limits for TNF alpha and IFN gamma were 80 pg / ml and 200 pg / ml , respectively . ^^^ The TNF alpha and IFN gamma values were compared with the serum levels of beta 2 microglobulin , calcium and creatinine , the M component , the erythrocyte sedimentation rate , the degree of plasma cell infiltration of the bone marrow , the degree of skeletal destructions and with patients survival . ^^^ No significant correlations could be observed between TNF alpha or IFN gamma and these variables of myeloma activity . ^^^ We conclude that detection of serum TNF alpha and IFN gamma levels in multiple myeloma appears to be without any clinical value . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Three of the four cytokine preparations ( Il 4 , TNF and IFN alpha / beta ) , however , increased the macrophages capacity to secrete H2O2 in response to either immune complex or PMA , IFN gamma alone did not affect H2O2 secretion . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The capacity of the cytokines gamma interferon ( IFN gamma ) , interleukin 4 ( IL 4 ) , and tumor necrosis factor ( TNF ) to activate tuberculostatic functions in murine bone marrow derived macrophages ( BMM phi ) was investigated . ^^^ Although TNF alone was ineffective , it showed synergy with IFN gamma in the stimulation of tuberculostasis . ^^^ Our data suggest that not only IFN gamma but also IL 4 and TNF participate in the control of mycobacterial growth . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin 1 ( IL 1 ) , tumor necrosis factor ( TNF ) , and interferon gamma ( IFN gamma ) inhibit insulin release and may be cytotoxic to isolated rodent pancreatic islets . ^^^ In this study we examined the effects of IL 1 , TNF , and IFN gamma on the viability and hormone secretion of islets isolated from adult human pancreas and maintained in monolayer culture . ^^^ IL 1 and TNF were cytotoxic to the islet cells ( 20 30 % cell lysis ) in a 51Cr release cytotoxicity assay , and IFN gamma had only small effects ( less than 10 % lysis ) . ^^^ IFN gamma ( 10 ( 3 ) U / mL ) acted synergistically with IL 1 and TNF , and the three cytokines added together produced maximal islet cell lysis ( 46 . 4 + / 4 . 3 % ) . ^^^ Assay of insulin and glucagon in the islet monolayers revealed that IL 1 , TNF , and IFN gamma inhibited both B and A cell secretory functions ; however , only IL 1 and TNF produced permanent decreases in insulin and glucagon contents in the islet cultures . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro combination of TNF with interferon alpha ( IFN alpha ) and IFN beta resulted in additive growth inhibitory effects , while IFN gamma enhanced the TNF mediated growth inhibition in a synergistic way . ^^^ TNF did not induce HLA DQ , but reduced the IFN gamma induced expression of HLA DR and HLA DQ . ^^^ We also found that TNF differentially modulates IFN gamma induced effects , thus suggesting its potential role in the regulation of inflammatory skin disorders . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1 production in response to LPS could be augmented by crude lymphokine , IFN gamma , or tumour necrosis factor ( TNF ) alpha . ^^^ TNF alpha induced a significant amount of IL 1 ( mainly cell associated ) directly but could also induce IL 1 secretion when combined with IL 2 or IFN gamma , or when in the presence of serum . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Soluble polyclonal anti TNF alpha antibodies in culture fluids after activation of macrophages with IL 2 and IFN gamma totally abrogated the expression of resistance to infection . ^^^ The T cell growth hormone IL 2 acts as cofactor with IFN gamma for induction of a macrophage antimicrobial activity , and TNF alpha may be the effector molecule for resistance to infection regulated by these two lymphokines . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These inhibitors partially blocked induction of nitrite release in macrophages activated with the combination of IFN gamma plus TNF alpha , but were incapable of inhibiting when macrophages were activated by the combination of IFN gamma plus LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our study shows that TNF can have opposite effects on Ia expression ( induced by IFN gamma ) on thioglycollate elicited peritoneal macrophages , depending on the length of time cells are treated and on the presence of other modulators . ^^^ In particular , TNF augmented early expression induced by IFN gamma but inhibited later expression . ^^^ TNF and PGE 2 also synergized to inhibit Ia expression induced on bone marrow derived and splenic macrophages by either IFN gamma or IL 4 . ^^^ TNF blocked the increase in FcR expression due to any combination of PGE 2 , IFN gamma , and IFN beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Effects of associated cytokines ( IL 1 , TNF alpha , IFN gamma and TGF beta ) on collagen and glycosaminoglycan production by cultured human synovial cells . ^^^ The production of collagen and glycosaminoglycans ( GAG ) was studied in cultured human synovial cells exposed to four cytokines , alone or in dual combination , namely interleukin 1 ( IL 1 ) , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) and transforming growth factor beta ( TGF beta ) . ^^^ This effect was reversed when the factor was associated with either IL 1 beta ( 100 500 pg / ml ) , TNF alpha ( 1 100 ng / ml ) or IFN gamma ( 100 U / ml ) . ^^^ Except IFN gamma which clearly inhibits the collagen production , the other cytokines IL 1 and TNF alpha were not very effective when tested separately , although they generally induced a small reduction in collagen amount . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The objective of this investigation was to determine the effects of the cytokines interleukin 1 beta ( IL 1 beta ) , interleukin 1 alpha ( IL 1 alpha ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) , alone or in paired combinations , on PGE production by near confluent human periodontal ligament ( PDL ) fibroblasts in vitro . ^^^ Cells ( 1 10 10 ( 5 ) ) were seeded in 12 10 75 mm tissue culture tubes and incubated with various doses of IL 1 beta , IL 1 alpha , TNF alpha , and IFN gamma , alone or in specific combinations , for 15 min , two , 12 , 24 , and 72 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of IL 1 activity by R LPS stimulated C3H / HeJ macrophages was unaffected by IFN gamma ; however , this cytokine significantly enhanced TNF alpha production by the same cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Indirect evidence from in vitro studies suggests that IFN gamma and TNF alpha inhibit and stimulate bone resorption , respectively , but contradictory results have emerged from the literature regarding the effects of IFN gamma on macrophage multinucleation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have studied the short and long term effects of human recombinant tumor necrosis factor ( TNF ) and TNF / recombinant human interferon gamma ( IFN gamma ) mixtures on A 549 human lung carcinoma cells maintained in organotypic culture . ^^^ Continuous treatments with 2 10 10 ; 2 10 10 ( 2 ) ; 2 10 10 ( 3 ) and 2 10 10 ( 4 ) U / ml TNF or with mixtures of TNF / IFN gamma at 2 10 10 ( 2 ) and 10 ( 3 ) U / ml , respectively , were administered . ^^^ No regrowth , however , occurred when the IFN gamma / TNF combination was used , and the nodules disintegrated completely on the 35th day of treatment without evidence of any cellular survival . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When these macrophages were obtained from infected patients or subjected to LPS treatment , they directly released cytotoxic amounts of TNF in the extracellular fluid after activation with IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon ( IFN ) alpha and IFN gamma inhibited JE virus production by the infected monocytes in the absence of anti JE virus antibody , but interleukin ( IL ) 1 alpha , IL 2 , IL 3 , granulocyte macrophage colony stimulating factor ( GM CSF ) , granulocyte CSF ( G CSF ) , and tumor necrosis factor ( TNF ) alpha did not show a significant inhibition . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These studies revealed : 1 ) application of IFN gamma reduces the number of L . monocytogenes and treatment with anti IFN gamma antibodies worsens listeriosis ; 2 ) in vitro , tuberculostatic and listericidal macrophage functions are activated by IFN gamma , IL 4 , and IL 6 ; 3 ) tumor necrosis factor ( TNF ) synergizes with IFN gamma ; 4 ) treatment with anti TNF antibodies worsens listeriosis and interferes with M . bovis induced granuloma formation ; 5 ) concomitant application of mycobacterial products and TNF induces necrotic reactions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Combining subthreshold doses of IFN gamma liposomes or IFN gamma with lipopolysaccharide synergistically enhanced the release of TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The spot ELISA technique has been used to enumerate the frequency of cells secreting tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) , isolated from biopsies of normal intestine and from biopsies of children with inflammatory bowel disease . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The murine adenocarcinoma cell line TA 3 synthesized nitrite from L arginine upon stimulation with gamma interferon ( IFN gamma ) associated with tumor necrosis factor ( TNF ) , and / or bacterial lipopolysaccharide ( LPS ) , but not with IFN gamma , TNF , or LPS added separately . ^^^ Cytosolic preparations from TA 3 M 2 cells treated for 24 or 48 h with IFN gamma , TNF , and LPS exhibited a reduced ribonucleotide reductase activity , compared to untreated control cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Whereas treatment with interferon gamma ( IFN gamma ) activated both single and co infected groups of M phi to kill T . gondii , treatment with TNF did not influence the rate of MAC growth in co infected M phi , in contrast with the inhibition of growth observed in MAC infected M phi . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The increased PGE production provoked by tumour necrosis factor alpha ( TNF alpha ) in RSC was also augmented by staurosporine , but , in contrast , the increases in cellular [ Fru ( 2 , 6 ) P 2 ] induced by transforming growth factor beta ( TGF beta ) and interferon gamma ( IFN gamma ) were diminished . ^^^ These findings suggest that IL 1 alpha and probably TNF alpha act via an intracellular mechanism different from that mediating the action of TPA , TGF beta and IFN gamma , and provide evidence that staurosporine is capable of amplifying the IL 1 signal . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We conclude that the synergistic action of interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) may contribute to inhibition , but that also other cytokines are responsible for the observed inhibition of BM derived GM CFC . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Study objective : 1 ) to investigate serum levels of tumor necrosis factor alpha in patients treated with recombinant interferon gamma and 2 ) to relate changes in TNF serum levels to other biological responses observed during treatment with interferon gamma ( IFN gamma ) . ^^^ Measurements and main results : Tumor necrosis factor alpha ( TNF alpha ) , IFN gamma and neopterin serum levels , monocyte counts in the peripheral blood and body temperature were measured immediately before and 4 , 24 , 48 , 72 , and 168 h after each application of IFN gamma . ^^^ Results indicated that elevated serum levels of TNF alpha are induced by 100 micrograms and 500 micrograms IFN gamma . ^^^ Changes in TNF serum levels did not correlate with the magnitude of febrile reactions , neopterin production or IFN gamma serum levels . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum concentration kinetics of gamma interferon ( IFN gamma ) , neopterin , 2 ' 5 ' A synthetase and tumor necrosis factor alpha were determined in five cancer patients undergoing adoptive immunotherapy with high dose interleukin 2 ( IL 2 ) bolus infusion and lymphokine activated killer cells according to the National Cancer Institute , NIH protocol . ^^^ This suggests that the antitumor effect of high dose IL 2 bolus administration may be in part mediated by activation of a cascade of endogenous cytokines including IFN gamma and tumor necrosis factor alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our data confirm the presence of synergism , which greatly enhances cell proliferation , IL 2 , tumour necrosis factor ( TNF ) and interferon gamma ( IFN gamma ) secretion by human mononuclear cells ( MNC ) and the production of IL 2 and TNF by mouse cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Priming the macrophage respiratory burst with IL 4 : enhancement with TNF alpha but inhibition by IFN gamma . ^^^ The opposing effects of TNF alpha and IFN gamma when interacting with IL 4 highlight the difficulties in predicting the effect of a given cytokine in vivo , where potential interactions with other cytokines must be considered . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
K 562 , which is B lymphoid cell line and lacks HLA class 1 molecules , showed detectable amounts of HLA class 1 molecules after exposure to interferon gamma ( IFN gamma ) and / or tumor necrosis factor alpha ( TNF alpha ) . ^^^ However , the treatment of sperm with IFN gamma and / or TNF alpha had no effect on the expression of HLA class 1 and class 2 molecules . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 4 has been shown to participate in interregulatory networks with other cytokines including IL 2 , IFN gamma , IL 1 beta , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , tumour necrosis factor ( TNF ) and interferon gamma ( IFN gamma ) , two cytokines released from mononuclear cells in response to IL 2 , also reduced the survival and thymidine uptake of malignant plasma cells in culture . ^^^ However , IL 2 infusions also induced substantial increases in the production of the cytokines TNF and IFN gamma from peripheral blood lymphocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To elucidate which cytokines produced by monocytes are controlled by IL 4 , we tested the effect of IL 4 on the secretion of IL 1 alpha , IL 1 beta , TNF alpha , and IL 6 induced by LPS or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Endotoxin stimulated release of significantly higher amounts of TNF alpha from macrophages from the IFN gamma treated group compared to macrophages from the control group . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , IL 3 , together with interferon gamma ( IFN gamma ) , stimulated the TNF alpha , but not IL 1 , activities of monocytes from several donors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The other 21 tumor cell lines examined were resistant to TNF alpha but , in their large majority , were more or less susceptible to killing by interferon gamma ( IFN gamma ) and Corynebacterium parvum ( CP ) activated BMMP . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In an effort to understand what other mechanisms might play a role in the patient with sepsis , we examined the effect of interferon gamma ( IFN gamma ) on the synthesis of cachectin / TNF . ^^^ We show here that IFN gamma , although unable by itself to induce cachectin / TNF synthesis , enhanced the endotoxin induced production of cachectin / TNF in vitro . ^^^ Furthermore , IFN gamma overcame the inhibition of cachectin / TNF synthesis caused by the glucocorticoid , dexamethasone . ^^^ These effects of IFN gamma were accounted for by increased levels of cachectin / TNF mRNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When mixtures of TNF and various other cytokines ( IL 1 , IL 2 , granulocyte CSF , IFN alpha , IFN beta , IFN gamma ) were injected , only IL 1 and IFN gamma augmented TNF induced vascular hyperpermeability , the increase occurring in a dose dependent manner . ^^^ The induction of vascular hyperpermeability by TNF and its enhancement by IL 1 and IFN gamma were inhibited by selective depletion of peripheral blood neutrophils with i . p . administration of an anti rat neutrophil mAb , RP 3 . ^^^ Vascular hyperpermeability induced by tumor necrosis factor and its augmentation by IL 1 and IFN gamma is inhibited by selective depletion of neutrophils with a monoclonal antibody . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Therefore , we investigated the effect of interferon alpha ( IFN alpha ) , IFN gamma and tumor necrosis factor ( TNF alpha ) on the induction of LAK activity by IL 2 , and the induction of IFN gamma and TNF alpha by IL 2 . ^^^ LAK activity could not be enhanced by IFN gamma and TNF alpha , but was enhanced by IFN alpha . ^^^ This may in part be due to the fact that IL 2 itself induces high amounts of TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lymphocyte adhesion to cerebral endothelium is normally low , but is sensitive to induction by interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Previous data from this laboratory had shown that the cytokine tumor necrosis factor alpha ( TNF alpha ) enhances IFN gamma mediated class 2 antigen expression on astrocytes . ^^^ We have now investigated the effect of IFN gamma and TNF alpha on class 2 MHC mRNA expression in astrocytes using Northern blot analysis . ^^^ This level of expression was further enhanced by the simultaneous addition of IFN gamma and TNF alpha to the astrocytes , while TNF alpha alone had no effect on class 2 mRNA expression . ^^^ TNF alpha does not act by increasing the stability of IFN gamma induced class 2 mRNA , indicating its action is not at that specific level of post transcriptional control . ^^^ Furthermore , astrocyte class 2 mRNA expression was inhibited when cycloheximide ( CHX ) was added together with IFN gamma or IFN gamma / TNF alpha , and when CHX was added up to 4 h after treatment with IFN gamma or IFN gamma / TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using human renal cell carcinoma heterotransplanted in nude mice ( JRC 11 : anaplastic and alveolar pattern , grade 4 ) , the in vivo anti tumour efficacy of tumour necrosis factor ( rHu TNF alpha : PT 050 ) and IFN alpha ( nHu IFN alpha : HLBI ) , IFN gamma ( nHu IFN gamma : OH 6000 ) were investigated . ^^^ Mono therapy of TNF , IFN alpha and IFN gamma was not effective with regard to tumour regression rates , histological degeneration rates and survival time of the host mice . ^^^ Combination therapy of TNF and IFN alpha , IFN gamma were also not effective with regard to tumour regression rates , but when considering histological change , sporadic disappearance of endothelium of intra tumoural vasculature , flow of tumour cells clustered in intra vascular cavity , and extra vascular bleeding were observed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cryostat sections of skin biopsies from 57 patients with various forms of leprosy were immunostained according to the APAAP method , using monoclonal antibodies against interleukin 1 beta ( IL 1 beta ) , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and , in addition , against CD 1 antigen . ^^^ Granulomas in biopsies of untreated patients with tuberculoid leprosy showed large amounts of cells positive for IL 1 beta , TNF alpha , IFN gamma , and CD 1 , whereas no positive signals could be detected in untreated patients with lepromatous leprosy . ^^^ Remarkably , in tuberculoid leprosy patients , the number of IL 1 beta positive cells did not vary under therapy , while the number of TNF alpha and IFN gamma reactive cells decreased . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma initiated heightened mRNA levels for JE but did not alter specific mRNA for IL 1 , TNF , or KC . ^^^ When IFN gamma and LPS were combined , additive effects on levels of specific mRNA for JE , enhancement of mRNA for TNF , suppressed mRNA for KC , and no effect on mRNA for IL 1 were observed . ^^^ When IFN gamma and LPS were given together , IFN gamma enhanced the LPS induced transcription of TNF and KC , suggesting decreased stability of mRNA for KC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The multipotential cytokine , transforming growth factor beta 2 ( TGF beta 2 ) , is as effective as glucocorticoids in suppressing the production of tumor necrosis factor alpha ( TNF alpha ) by lipopolysaccharide ( LPS ) stimulated macrophages , and this inhibition can be abrogated by exogenous interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A modulation of IL 6 production in ECM was achieved by in vivo treatment with other anticytokine antibodies : antibodies to interferon ( IFN gamma ) or to tumor necrosis factor ( TNF ) abolished the rise of IL 6 , while anti IL 3 and anti granulocyte / macrophage colony stimulating factor antibodies only partially prevented this rise , suggesting that the two cytokines IFN gamma and TNF are important intermediates in IL 6 production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study two crossreactive monoclonal antibodies , one specific for human IFN gamma ( MD 1 ) , the other specific for human TNF alpha ( 61E71 ) , were tested for immunosuppressive potencies in a skin transplantation study in the rhesus monkey . ^^^ This is the first reported demonstration of a synergistic effect of mAbs specific for IFN gamma and TNF alpha in the suppression of allograft rejection in primates . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This hypothesis was tested by exposing cultured rat anterior pituitary cells to murine tumor necrosis factor alpha ( TNF alpha ) and / or interferon gamma ( IFN gamma ) for 24 h before a 4 h test of cell function . ^^^ Overall prolactin accumulation during this first 24 h was inhibited by TNF alpha and markedly reduced by TNF alpha plus IFN gamma . ^^^ Cellular viability ( assessed by trypan blue and chromium release assays ) and prolactin cell content were unchanged after TNF alpha or IFN gamma treatment . ^^^ We conclude that both TNF alpha and IFN gamma have the potential to act directly on anterior pituitary cells to slow the rate of prolactin release . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study we have demonstrated good titres of tumour necrosis factor ( TNF ) and interferon gamma ( IFN gamma ) in the supernatants , which accounted for their observed cytostatic activity on the tumour targets . ^^^ The high level of cytostasis seen with the B 16 melanoma using the supernatants could be attributed to their sensitivity to the cytostatic activity of IFN gamma ; the lower levels of cytostasis seen with the IFN gamma resistant MC6A target was the result of IFN gamma increasing the sensitivity of this target to TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The suppressive action of both cytokines was accompanied by a reduction in tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) levels in lymphocyte culture supernatants . ^^^ Recombinant human IFN gamma ( rhIFN gamma ) , but not recombinant human TNF alpha ( rhTNF alpha ) was able to overcome the inhibitory effect of recombinant human interleukin 4 ( rhIL 4 ) on LAK induction and DNA synthesis but not Tac antigen expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This enhancement of gene expression was suppressed by tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) , as determined at mRNA steady state level , but two distinct mechanisms were involved . ^^^ TNF alpha suppressed the pro alpha 2 ( 1 ) collagen promoter activity , whereas IFN gamma had only a minimal effect at transcriptional level . ^^^ The effects of TNF alpha and IFN gamma were synergistic , suggesting that combination of these two factors may potentially provide pharmacologic means to counteract tissue deposition of collagen in diseases involving TGF beta . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma and TNF alpha were shown to increase bactericidal activity in an additive fashion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Possible parasite and cell associated factors that could contribute to the induction of nitrogen oxidation and cytotoxic activity of IFN gamma treated macrophages were examined : only certain intact microorganisms , LPS from a variety of bacteria , and the cytokine TNF alpha were effective . ^^^ TNF alpha was produced by amastigote infected macrophages and IFN gamma dramatically enhanced secretion of this cytokine ; IFN gamma alone had no effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effect of IFN gamma is drastically increased by cotreatment with tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 ( IL 1 ) , or lipopolysaccharide ( LPS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Analysis of the supernatant culture medium after 72 h preincubation demonstrated that HM BP induced release of interferon gamma ( IFN gamma ) from T cells ( preferentially from CD3+CD4+ cells ) and of tumour necrosis factor alpha ( TNF alpha ) from monocytes / macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The combination of interferon gamma ( IFN gamma ) with TNF alpha or IL 1 beta enhanced the induction of class 1 and class 2 antigens , compared with the effect of IFN gamma alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumour necrosis factors alpha and beta ( TNF alpha and TNF beta ) and gamma interferon ( IFN gamma ) were measured by ELISA in the supernatants of phytohaemagglutinin ( PHA ) activated peripheral blood mononuclear cells ( PBMNC ) from 98 individuals ( 60 controls and 38 patients with insulin dependent diabetes mellitus [ IDDM ] ) . ^^^ In our population study we observed a correlation between the levels of secretion of TNF alpha and IFN gamma but not TNF beta . ^^^ Reduced levels of TNF beta , but not TNF alpha or IFN gamma , secretion were found in IDDM patients stimulated with 1 and 5 micrograms / ml of PHA ( P = 0 . 001 and 0 . 02 respectively ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Endogenous production of interferon gamma ( IFN gamma ) and tumour necrosis factor ( TNF ) in the bloodstreams induced by the infection was normal in DSG treated mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The contribution of the T cell and macrophage derived cytokines , interferon gamma ( IFN gamma ) and tumor necrosis factor ( TNF ) , respectively , in the cell mediated mechanisms leading to acquired immunity to blood stage Plasmodium chabaudi AS was investigated . ^^^ In conclusion , both IFN gamma and TNF appear to contribute to host resistance to blood stage infection with P . chabaudi AS . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This participation is mediated by parasite induction of tumor necrosis factor alpha ( TNF alpha ) , an autocrine factor of macrophages : NO synthesis by interferon gamma ( IFN gamma ) treated cells can be blocked by monoclonal antibodies to TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines , TNF alpha , IFN gamma and TNF beta at very high concentrations were not toxic to P . falciparum in culture . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These cytokines , TNF and IFN gamma , require additional , yet unidentified complementary factors that are present in `` crisis ' ' and `` paroxysm ' ' serum to kill intra erythrocytic blood stage parasites . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We examined the antitumor effect of glycosylated recombinant lymphotoxin ( LT ) in combination with human interferon gamma ( IFN gamma ) on human tumors transplanted into nude mice and compared it with that of tumor necrosis factor ( TNF ) . ^^^ The effect was observed after either i . v . or s . c . injection . ( 2 ) In contrast , no synergistic or additive effect on HeLa S 3 tumors was observed in the case of TNF combined with IFN gamma . ( 3 ) The serum half life of glycosylated LT in tumor bearing mice was about 22 fold longer than that of TNF . ^^^ In conclusion , glycosylated LT , especially in combination with IFN gamma , appears to be a potent cytokine against tumor growth in vivo compared with TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TcR 5 gamma and 5 delta gene expression was determined in 40 TcR delta 1+ clones using the gamma delta T cell subset markers Ti gamma A and delta TCS 1 , in conjunction with Southern blot analysis using TcR J gamma and J delta probes . gamma delta T cell clones , together with control alpha beta T cell clones derived from the same lymphocyte populations , were stimulated with phytohaemagglutinin ( PHA ) and their ability to produce interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) tested using specific ELISA . ^^^ There was no relationship between levels of IFN gamma and TNF alpha produced by individual gamma delta T cell clones and also no relationship between their non major histocompatibility complex ( MHC ) restricted cytotoxic activity and levels of either cytokine . ^^^ There was a significant tendency for gamma delta T cell clones to produce more TNF alpha than IFN gamma in comparison to alpha beta T cell clones . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Factors which clearly contribute to the suppression of erythropoiesis are ( a ) reduced iron availability and ( b ) stimulation of the synthesis of immunomodulatory peptides such as IL 1 , TNF alpha and IFN gamma , which inhibit the proliferation of erythrocytic progenitors in the bone marrow . ^^^ The present in vitro studies show that IL 1 and TNF alpha , but not IFN gamma significantly lower the pO 2 dependent formation of EPO in HepG 2 cultures . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of Transforming Growth Factor beta 1 ( TGF beta ) , Tumor Necrosis Factor alpha ( TNF alpha ) , Interferon gamma ( IFN gamma ) and LIF HILDA were compared on leukemic cells cultured in vitro for seven days . ^^^ Recombinant TNF alpha and IFN gamma were found to induce either inhibition or enhancement of the proliferation on separate specimens . ^^^ Both TNF alpha and IFN gamma were found to enhance the expression of adhesion molecules and to a lesser extent , the expression of some lineage associated antigens . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
HLA DR , DP , DQ induction in human islet beta cells by the cytokine combination IFN gamma + TNF alpha . ^^^ It has been demonstrated that human beta cells can be induced to express Class 2 when cultured with IFN gamma + TNF alpha or IFN gamma + TNF beta . ^^^ To assess whether or not they can be induced to express the products of the Class 2 subregions , DR , DP and DQ , human islet cultures from 10 pancreas were supplemented with the combination of IFN gamma + TNF alpha using MoAbs specific for DR , DP and DQ products , and antibodies to insulin and glucagon . ^^^ The combination IFN gamma + TNF alpha ( 100 1000 U / ml each ) was able to induce the expression of the three subregions in both beta and alpha cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , interferon gamma ( IFN gamma ) , which induced major histocompatibility complex ( MHC ) class 2 expression , did not induce IL 1 or TNF alpha production . ^^^ However , IFN gamma enhanced the cell surface expression of HLA DR and the production of IL 1 and TNF alpha on monocyte enriched cells stimulated by GM CSF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Injection of IFN gamma alone did not affect the levels of TNF and TNF BP . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) were examined for effects on human squamous carcinoma cells . ^^^ IFN gamma inhibited proliferation at concentrations between 100 and 1 , 500 units / ml and the inhibitory effects were potentiated by TNF alpha . ^^^ These findings indicate that malignant squamous epithelial cells may be similar to their normal counterparts in their responses to IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human PDL cells were seeded in tissue culture tubes and incubated with interleukin 1 alpha ( IL 1 alpha ) , IL 1 beta , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , indomethacin , parathyroid hormone ( PTH ) , or their combinations , for 1 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The rosetting M phi subset that expresses high densities of FcRI ( FcRI+ ) produced the majority of normal human peripheral blood M phi TNF whether the stimulation was an interferon gamma ( IFN gamma ) prime followed by MDP or followed by interleukin 2 ( IL 2 ) . ^^^ The difference in the M phi subset ' s TNF response remained even after the FcRI M phi subset received a 2 . 5 fold increase in stimulation with the classical M phi induction regimen of IFN gamma plus bacterial cell wall product . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The high level of transcripts of IFN gamma and TNF alpha genes observed in freshly isolated B220+ / Thy1+ cells decreased after 10 hr of in vitro culture , while levels of TNF beta , IL 6 and TGF beta transcripts were maintained . ^^^ These results suggest that a positive stimulus for IFN gamma and TNF alpha gene transcription by lpr B220+ / Thy1+ cells may exist in vivo but is removed upon purification of this abnormal T cell subset . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
GM CSF , TNF , and IFN gamma treatment enhanced killing of HC 32 and HC IV . 3 by in vitro cultured monocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Plasma tumor necrosis factor alpha ( TNF alpha ) levels , but not interferon gamma ( IFN gamma ) levels , increased during IL 2 treatment , but spontaneous and IL 2 stimulated TNF alpha secretion in vitro remained abnormally low . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This activation induces modulation of the CD 16 molecule and release of IFN gamma and TNF alpha by NK cells . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the cytotoxic effects of recombinant tumor necrosis factor ( TNF ) alone and in combination with interferon gamma ( IFN gamma ) and / or cytotoxic drugs on a variety of human tumor cell lines ( U 937 , IGROV 1 , HT 29 , LoVo , MCF 7 and U20S ) , including cell lines with in vitro acquired resistance ( LoVo / DX and MCF7 / DX selected for resistance to doxorubicin ( DX ) and characterized by pleiotropic drug resistance ; U20S selected for resistance to cisplatin ( CDDP ] , using MTT assay . ^^^ Treatment with increasing doses of TNF after 6 h incubation with a subtoxic concentration of IFN gamma produced a synergistic effect in four cell lines ( U 937 , HT 29 , LoVo / DX and MCF 7 ) , whereas in the other five the cell killing of the combination was comparable with that achieved by TNF alone . ^^^ The combination of TNF and IFN gamma enhanced cytotoxicity about 20 fold for DX and 6 fold for CDDP , evaluated in terms of the modification index , against LoVo / DX and U20S / Pt cells respectively . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The addition of PAF to freshly isolated PBM triggered the release of tumor necrosis factor ( TNF ) in the supernatant in the absence of induction of macrophage mediated cytotoxicity , unlike interferon gamma ( IFN gamma ) or lipopolysaccharide ( LPS ) . ^^^ PBM primed with PAF respond by secreting TNF to both phorbol myristate acetate and concanavalin A but respond poorly to PAF , LPS , or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
WiDR colorectal carcinoma cells are highly sensitive to the synergistic cytotoxic effects of tumor necrosis factor ( TNF ) and gamma interferon ( IFN gamma ) . ^^^ In the present study , we have investigated the effects of recombinant human ( rh ) TNF and IFN gamma on the binding of both ligands in this cell line . ^^^ These data indicate that in some cell types TNF and IFN gamma may induce an increase in their own receptors ( homologous up regulation ) and concomitantly increase each other ' s receptors ( heterologous up regulation ) and that these actions are due , in part , to enhanced receptor synthesis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The H 161 cells were treated with differentiation inducing agents , i . e . , sodium butyrate , hemin , retinoic acid , DMSO , vitamin D 3 , and PMA alone or in combination with IL 1 alpha , IL 2 , IL 3 , IL 4 , IL 5 , IL 6 , TNF alpha , IFN gamma , granulocyte CSF , macrophage CSF , granulocyte / macrophage CSF ( GM CSF ) , and Con A and tested for the production of IL 1 inhibitor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF , IL 1 , IFN gamma and PGE 2 secretion of peritoneal macrophage obtained by peritoneal washing at the first day after laparotomy from 19 patients with cervical cancer and 19 with ovarian cancer , who were treated with sizofiran ( SPG ) intramuscularly before laparotomy or with recombinant interferon gamma ( rIFN ) intraperitoneally at the day of laparotomy , were studied . ^^^ Treatment with SPG and rIFN significantly increased the number and secretion of TNF , IL 1 and IFN gamma ( p less than 0 . 05 ) but not that of PGE 2 . ^^^ A high correlation between secretion of TNF and IFN gamma ( r = 0 . 99 , p less than 0 . 01 ) was observed . ^^^ These data suggest that potent tumoricidal activity of peritoneal macrophage obtained from the patients with gynecological malignancies could be expected by the treatment with SPG and rIFN due to the increased secretion of TNF , IFN gamma and IL 1 and inhibition of increased secretion of PGE2 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We recently reported that interleukin 2 ( IL 2 ) activated peripheral blood lymphocytes and CD3+ , lymphokine activated killer ( LAK ) cell clones release tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) when stimulated with K 562 erythroleukemia cells . ^^^ We examined the phenotype of IL 2 activated peripheral blood leukocytes that secrete TNF alpha and IFN gamma when stimulated with K 562 cells and demonstrated that TNF alpha secretion is not due to the presence of contaminating mononuclear phagocytes . ^^^ Further , we demonstrate that IL 2 activated natural killer ( NK ) cells release only IFN gamma when stimulated with K 562 cells while T lymphocytes exposed to monoclonal anti CD 3 and K 562 cells secrete both TNF alpha and IFN gamma . ^^^ However , T cells stimulated only with K 562 cells did not release IFN gamma or TNF alpha while the admixture of these T cells with NK cells , when stimulated with K 562 cells , released levels of TNF alpha comparable to those produced by the unseparated cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Low doses of recombinant murine interferon gamma ( IFN gamma ) plus recombinant human tumor necrosis factor alpha ( TNF alpha ) were used to limit myelopoiesis and , in turn , reduce the presence of myelopoiesis associated immune suppressor cells in LLC C 3 tumor bearers . ^^^ Neither IFN gamma nor TNF alpha alone had any effect in vitro on the growth of myeloid progenitor cells into colonies or on the suppressive activity of bone marrow cells from LLC C 3 bearing mice . ^^^ However , the combination of low doses of IFN gamma and TNF alpha synergistically inhibited both the growth of myeloid progenitor cells into colonies and the suppressive activity of bone marrow cells from tumor bearers . ^^^ When used alone , neither IFN gamma nor TNF alpha had any effect on myelopoiesis or on suppressor cell activity . ^^^ When combined , IFN gamma plus TNF alpha synergistically suppressed myelopoiesis and the presence of immune suppressive cells both in the bone marrow and in the spleen of tumor bearers . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The biologic relevance of IFN gamma receptor phosphorylation was suggested by three additional observations ; 1 ) there was a close correlation between the extent of receptor phosphorylation and the magnitude of the cellular response induced , 2 ) TNF alpha concomitantly enhanced both IFN gamma dependent HLA DR expression and IFN gamma dependent receptor phosphorylation on Colo 205 , and 3 ) phosphorylation of functionally inactive recombinant murine IFN gamma receptors expressed on transfected human 293 or Colo 205 cells was not induced by murine IFN gamma but was induced by the homologous human ligand . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , PHA alone or in combination with PMA induced tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) ( but not IL 2 ) production by CD 3 thymocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Class 1 expression was dose dependently augmented by incubation with interferon gamma ( IFN gamma ) or tumor necrosis factor ( TNF ) . ^^^ Although neither IFN gamma nor TNF alone induce class 2 molecules on islet cells , synergistic interaction of IFN gamma ( 200 U / ml ) and TNF ( 200 U / ml ) may induce class 2 expression on approximately 50 % of islet cells . ^^^ Niacinamide and 3 aminobenzamide , both inhibitors of ADP ribosylation and scavengers of free radicals , attenuated the class 2 expression induced by IFN gamma and TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The lymphokines IL 2 and IL 4 promoted the growth of human PHA triggered T cells , but only IL 2 induced the production of IFN gamma and TNF . ^^^ The addition of purified monocytes strongly enhanced the production of IFN gamma in IL 2 stimulated T cell cultures but did not influence the production of TNF or the level of T cell proliferation . ^^^ The addition of IL 1 to T cells activated by PHA and optimal concentrations of IL 2 resulted in a strong induction of IFN gamma production but had no influence on TNF production or T cell proliferation . ^^^ IL 6 did not influence IFN gamma or TNF production or T cell proliferation induced by PHA IL 2 and did not modulate IL 1 induced IFN gamma production . ^^^ We demonstrate , at the clonal level , that the optimal production of IFN gamma by human Th cells requires both IL 1 and IL 2 , whereas the production of TNF and T cell proliferation are induced by IL 2 alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Antibodies to TNF alpha and to IFN gamma almost completely inhibited the lysis seen on Me665 / 2 ( but not on K 562 ) , in 96 h assays , by supernatants isolated from VSKB LCL or anti CD 3 stimulated CTL 49 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mouse islet cell monolayers were damaged when cultured for five days in a medium containing 200 U / ml of recombinant murine interferon gamma ( IFN gamma ) and 300 U / ml of recombinant tumor necrosis factor ( TNF ) . ^^^ When 20 mM of nicotinamide or 5 mM of 3 aminobenzamide was supplemented to the medium , islet cell monolayers remained in the presence of the cytokines . 51Cr release studies showed that specific 51Cr release during five day incubation with 200 U / ml of IFN gamma and 300 U / ml of TNF was 30 + / 4 % ( mean + / SE ) . ^^^ In a medium containing 20 mM of nicotinamide , together with IFN gamma and TNF , specific 51Cr release was significantly reduced ( 12 + / 3 % , p less than 0 . 01 ) . 3 aminobenzamide was effective at the level of 5 mM ; specific 51Cr release was 2 + / 5 % ( p less than 0 . 01 ) . ^^^ These results suggest that the mechanism by which IFN gamma and TNF damage islet cells may be similar to that of streptozotocin and / or alloxan . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neither drug affected binding of IFN gamma to macrophages , nor binding of TNF alpha to human polymorphonuclear leukocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Alterations in macrophage oxidative burst activity following in vivo administration of recombinant murine gamma interferon ( IFN gamma ) , recombinant murine tumor necrosis factor alpha , and the antifungal antibiotic amphotericin B were investigated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In order to investigate the effect of tumor necrosis factor ( recombinant TNF alpha ) on hepatoma , we assessed the antitumor activity of TNF alpha on hepatoma cell line , PLC / PRF / 5 in vitro , and combined effects of interferon gamma ( IFN gamma ) , that is thought to increase TNF receptors . ^^^ On the other hand , treatment of IFN gamma combined with TNF alpha inhibited DNA synthesis and production of HBsAg . ^^^ According to these findings , antitumor activity of TNF alpha had no effect on PLC / PRF / 5 in itself , but was significantly enhanced when treated with IFN gamma at the same time . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of TNF receptors appeared to be regulated by other cytokines such as IFN gamma and IL 2 , with IFN gamma down regulating receptor expression on all cell types , and IL 2 up regulating receptor expression on lymphocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found that the addition of IFN gamma and tumor cells ( either the A 375 or HT 29 cells ) to monocytes induced TNF release , whereas IFN alpha or IFN gamma alone or IFN alpha and tumor cells had no effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The addition of interferon gamma ( IFN gamma ) up regulated the expression of TNF receptors in differentiated M 1 cells and Mm 1 cells , while immature M 1 cells were insensitive to IFN gamma . ^^^ The number of TNF receptors on the differentiated cells was increased 4 5 fold by the treatment with IFN gamma with no change in the binding constant . ^^^ Enhancement of TNF mediated cytotoxicity by the treatment with IFN gamma did not correlate with increases in the number of TNF receptors . ^^^ Both the constitutive expression and IFN gamma mediated superinduction of TNF receptors , and the constitutive and LPS induced secretion of TNF alpha were closely related to the extent of cellular differentiation along the monocytic pathway . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of 397 R 4 with IFN alpha or IFN gamma , + / TNF alpha for 72 h before cytolytic assays enhanced TIL lysis of this target slightly , and enhanced surface expression of MHC class 1 and 2 molecules and the adhesion molecule ICAM 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Of the other cytokines tested [ interleukin 1 ( IL 1 ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 6 ( IL 6 ) , and interferon alpha / beta ( IFN alpha / beta ) ] only TNF alpha and IL 6 were found to inhibit CRF stimulated ACTH release , although this inhibition was less pronounced than that caused by IFN gamma . ^^^ These results identify IFN gamma as one of the inflammatory cytokines that , like IL 1 , TNF alpha , and IL 6 , have the potential to regulate pituitary function . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In seven out of nine patients with acute cellular rejections , elevated levels of TNF alpha , IFN gamma , IL 1 , B2M , and neopterin were detected . ^^^ Our results indicate that enhanced endogenous production of TNF alpha and IFN gamma accompanied by elevated levels of B2M and neopterin represent a regular feature of inflammatory complications after liver transplantation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of U 937 cells with IFN gamma enhanced ( 2 ' 5 ' ) oligo ( A ) synthetase activity ( 2 . 5 fold ) but treatment with TNF did not . ^^^ Combined treatment with TNF alpha + IFN gamma increased this activity dramatically ( 20 40 fold ) . ^^^ Furthermore , binding studies and cross linking analysis showed that IFN gamma modulated TNF alpha receptors in U 937 cells without altering their properties , but did not do so in HL 60 cells . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Macrophage responses to recombinant IFN gamma decline during aging , as measured by two criteria of macrophage activation , O 2 and TNF alpha secretion . ^^^ Furthermore , the secretion of TNF alpha in response to IFN gamma and LPS is almost absent in macrophages from aged rats . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our results show that both IFN gamma and TNF alpha mRNA levels increased in U 937 cells upon poliovirus infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human IL 1 alpha , tumor necrosis factor alpha , IL 4 , rat IL 3 and rat IFN gamma , as well as L cell conditioned medium containing M CSF , lack enhancing activity . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1 , TNF , and IL 2 had no influence , whereas IFN gamma only induced Ia . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Most likely candidates for connective links are interferon gamma ( IFN gamma ) , granulocyte monocyte colony stimulating factor ( GM CSF ) and tumor necrosis factor ( TNF ) . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our observations indicate that TNF alpha could act in an autocrine fashion to induce the proliferation of this malignant glioma cell line and that TNF alpha exerts its effect by binding to specific TNF alpha receptors whose expression was enhanced by IFN gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The following recombinant cytokines were used : IL 1 beta , IL 2 , IL 3 , IL 4 , IFN gamma , tumor necrosis factor ( TNF ) alpha , GM CSF , and macrophage colony stimulating factor ( M CSF ) . ^^^ TNF alpha inhibited IFN gamma mediated HLA DR expression ( 46 . 7 + / 4 . 1 % inhibition ) and HLA DR mRNA accumulation . ^^^ IFN gamma ( but none of the other cytokines ) inhibited TNF alpha induced proliferation ( 70 + / 14 % inhibition ) without affecting the activity of IL 1 . ^^^ IFN gamma decreased TNF alpha mediated collagenase production ( 69 + / 19 % inhibition ) and GM CSF production but had no effect on the action of IL 1 . ^^^ These data demonstrate mutual antagonism between IFN gamma and TNF alpha on fibroblast like synoviocytes and suggest a novel homeostatic control mechanism that might be defective in RA where very little IFN gamma is produced . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pretreatment of the parental MC 38 tumor cell line with tumor necrosis factor ( TNF ) and interferon gamma ( IFN gamma ) increased its susceptibility to lysis by the BF 1 TIL line , but not to lysis by lymphokine activated killer cells , in in vitro cytotoxicity assays . ^^^ In experiments testing the ability of TILs to treat established lung metastases , the combination of TNF , IFN gamma , IL 2 , and TILs was shown to increase significantly the antitumor properties of this therapy when compared to TILs and IL 2 . ^^^ This result demonstrates that combinations of lymphokines , which when administered alone do not affect micrometastatic tumor burdens ( TNF , IFN gamma ) , can synergize with cellular immunotherapy in the treatment of established tumor burdens and may have applicabilities to the treatment of cancer in humans . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The doses of IFN gamma and TNF alpha used were suboptimal in that either cytokine alone did not have any effect . ^^^ The therapeutic effect of IFN gamma and TNF alpha in vivo is reflected in their ability to activate macrophages to kill the intracellular parasites in vitro . ^^^ The macrophage leishmanicidal activity induced by TNF alpha and IFN gamma can be completely inhibited by a specific inhibitor ( L NG monomethyl arginine ) of nitric oxide synthesis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma enhanced PGE 2 secretion , and this effect of IFN gamma was stronger than that of IL 1 or TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The activity of BSA was not substituted by IL 1 , IL 2 , IL 4 , IFN gamma , TNF , NGF or erythropoietin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Of the factors tested ( PDGF , aFGF , bFGF , EGF , TGF beta , IL 1 alpha , TNF alpha and IFN gamma ) , PDGF , was clearly the most potent stimulant of long term growth of both rat and human synoviocytes . ^^^ EGF , TGF beta , IL 1 alpha , TNF alpha , and IFN gamma had minimal effects . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In response to antigenic stimulation , T cells and macrophages secrete a set of glycoproteins termed lymphokines and monokines such as IL 1 , IL 2 , IL 3 , IL 4 , IL 5 , IL 6 , GM CSF , G CSF , IFN alpha , IFN gamma , TNF alpha , and lymphotoxin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The strongest differentiation inducing activity on promyelocytic HL 60 cells and histiocytic U 937 cells was obtained by combining recombinant tumor necrosis factor ( rTNF ) , interferon gamma ( IFN gamma ) , retinoic acid ( RA ) , and 1 alpha , 25 dihydroxyvitamin D 3 ( 1 alpha , 25 ( OH ) 2D3 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The principal cytokines that have been shown to affect skeletal tissues include factors previously described as monokines or lymphokines such as interleukin 1 ( IL 1 ) , tumor necrosis factors ( TNF alpha and TNF beta ) , and interferon gamma ( IFN gamma ) ; the colony stimulating factors ; and the so called growth and differentiation factors including transforming growth factors alpha and beta ( TGF alpha and TGF beta ) , insulinlike growth factor 1 ( IGF 1 ) , platelet derived growth factor ( PDGF ) , and fibroblast growth factor ( FGF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Several recombinant cytokines ( TNF alpha , IL 1 alpha , IL 1 beta , IL 6 , interferon gamma ( IFN gamma ) , as well as poly ( rI . rC ) were effective inducers of hepatic MT 1 and MT 2 genes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Competitive experiments showed that MNSF bound to the specific receptor and that the binding was not shared with IL 2 , IFN gamma , and TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The extravasation of lymphocytes from blood into non lymphoid tissues can be induced in the skin with IFN gamma and particularly tumor necrosis factor ( TNF ) alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neither ethanol nor tumor necrosis factor ( TNF ) alone , or together with IFN gamma head significant effects on MHC expression by TM 3 and TM 4 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The pathogenesis of cerebral malaria in mice is believed to be due to an immunopathological reaction giving rise to an excessive production of cytokines such as interferon gamma ( IFN gamma ) and tumor necrosis factor ( TNF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
By Northern blot analysis , it was shown that the induction of mRNA for IFN alpha , IFN gamma , and tumor necrosis factor alpha by FAA in the spleen cells of mice was significantly reduced in alkalinized mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We describe a non operative case in which a combined use of embolization , IFN gamma and tumor necrosis factor ( TNF ) elicited a lasting partial response , and an operative case in which postoperative complications such as pulmonary infarction and renal failure occurred after operation under extracorporeal circulation and patient died at 2 months after operation . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , tumor necrosis factor ( TNF ) production ( IFN gamma plus MDP induced ) of the CD4+ MO subset was slightly greater than that of the CD 4 MO . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Recombinant derived TNF exhibited direct toxicity to schistosomula at high concentrations , or at lower concentrations in the presence of IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Of the cytokines tested , which included interleukin 1 ( IL 1 ) , interleukin 2 ( IL 2 ) , interleukin 3 ( IL 3 ) , interleukin 4 ( IL 4 ) , interleukin 6 ( IL 6 ) , interferon alpha ( IFN alpha ) , interferon gamma ( IFN gamma ) , transforming growth factor alpha ( TGF alpha ) , transforming growth factor beta ( TGF beta ) , platelet derived growth factor ( PDGF ) , and tumor necrosis factor alpha ( TNF alpha ) , only TNF alpha and PDGF had demonstrable growth factor activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha , but not IL 1 , IL 2 , IL 6 , or IFN gamma , enhanced the prostaglandin E 2 production when added in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The hybridoma derived factor , termed the T cell enhancing factor ( TCEF ) , was functionally distinguishable from the defined cytokines IL 1 through IL 6 , IFN gamma , and TNF by bioassay . ^^^ Furthermore , RNA derived from the hybridoma failed to hybridize with cDNA probes specific for IL 1 to IL 6 , IFN gamma , TNF alpha , and granulocyte macrophage CSF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although perforin ( hemolytic ) activity was detected in unstimulated R8i , it was induced 2 to 14 fold in the presence of IL 2 , IL 3 , IL 4 , and IL 6 , and to a lesser degree ( less than 4 fold ) by TNF and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , by ELISA and radioimmunoassays , antibodies against human alpha LT , tumor necrosis factor , and IFN gamma did not react with IL 1 , indicating that IL 1 is antigenically distinct from these other cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Injection of recombinant IFN gamma with OK 432 or of IFN alpha / beta , recombinant IFN beta , recombinant IFN alpha A / D or recombinant IL 2 six hours before OK 432 enhanced TNF production about 10 fold , which indicated priming actions of these compounds in TNF production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Further , in a priori HLA negative tumor cells , TNF alpha enhances IFN gamma induced class 1 and class 2 HLA gene expression . ^^^ In vitro transcription , Northern blot , and immunofluorescence analysis performed with the leukemic cell line K 562 reveal that TNF alpha reversibly enhances IFN gamma induced HLA gene expression at the level of mRNA transcription . ^^^ Therefore , it is conceivable that TNF alpha , similar to IFN gamma , might provoke tumor rejection not only via direct tumoricidal action but also via enhancement of HLA antigens and induction of tumor specific immune responses . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The lysis of fresh human tumor cells by MCF was not inhibited by monoclonal antibodies directed against TNF , lymphotoxin ( LT ) , IFN alpha , IFN gamma , or interleukin 1 ( IL 1 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The i . v . injection of recombinant IFN gamma ( rIFN gamma , 10 ( 4 ) U per mouse ) increased TNF production to 790 U / ml ; this priming effect was observed just after its injection , was maximal after 2 to 6 h , and disappeared after 24 h . ^^^ Recombinant IL 2 ( 10 ( 6 ) U per mouse ) had priming activity that showed a similar time course to that of interferons other than IFN gamma ( a maximal TNF production : 100 U / ml ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These studies demonstrate that IFN gamma , TNF , and LT are important stimulators of fibroblast GAG biosynthesis , that interactions between these cytokines may be important in this regulatory process , that these cytokines predominantly stimulate hyaluronic acid production and that this effect may be mediated by stimulation of fibroblast hyaluronate synthetase activity . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Herein we demonstrate that IFN alpha , IFN gamma , and IL 2 can induce human peripheral blood monocyte mediated lysis of tumor cells that are resistant to both the direct effects of TNF and to monocytes activated by TNF . ^^^ Neither induction of TNF receptors by IFN gamma nor inhibition of RNA synthesis by actinomycin D increased the susceptibility of TNF resistant tumor targets to TNF mediated monocyte cytotoxicity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , TNF and LT differ from IFN alpha / beta in that the former synergize with IFN gamma for class 1 regulation whereas the latter do not . ^^^ Furthermore , LT or TNF do not affect IFN gamma mediated class 2 induction at optimal class 1 inducing concentrations ( 100 U / ml ) , whereas IFN alpha and IFN beta ( at their optimal concentrations of 1 , 000 U / ml ) are strikingly inhibitory . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Rat brain endothelial cells were cultured as confluent monolayers and stimulated with combinations of interferon beta ( IFN beta ) , interferon gamma ( IFN gamma ) , tumour necrosis factor ( TNF ) and interleukin 1 ( IL 1 ) . ^^^ TNF by itself enhances class 1 expression and it synergises with IFN gamma in enhancing class 1 expression and inducing class 2 . ^^^ IFN gamma is the most potent modulator of MHC expression on these cells , although TNF acts as a strong co stimulator , while IL 1 and IFN beta have only minor effects . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human cytokine cDNAs have been isolated from a cDNA library made from normal human peripheral blood leukocytes ( PBLs ) treated with Sendai virus and the relative induction of tumor necrosis factor ( TNF ) , alpha and gamma interferons ( IFN alpha , IFN gamma ) , and interleukin 1 beta IL 1 beta ) genes has been analyzed . ^^^ In the Sendai virus induced PBL system , IL 1 beta mRNA was shown to be approximately twofold higher than TNF or IFN alpha mRNA whereas IFN gamma mRNA was 50 100 fold lower than TNF or IFN alpha mRNA . ^^^ The cytotoxic activity of TNF was analyzed on several cell lines and IFN alpha and IFN gamma were shown to potentiate TNF cytotoxicity about 2 200 fold depending on cell lines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The influence of purified recombinant human TNF alpha ( rhuTNF alpha ) was assessed , alone and in combination with purified recombinant human IFN gamma ( rhuIFN gamma ) , for its effects on enhancing release from human T lymphocytes of activities that stimulate colony formation by granulocyte macrophage , erythroid , and multipotential progenitor cells . rhuTNF alpha or rhuIFN gamma enhanced the release of CSF , which were determined to be granulocyte CSF and granulocyte macrophage CSF by human bone marrow colony assays , morphologic assessment of colony types , and neutralization studies with rabbit anti human granulocyte CSF and monoclonal mouse anti human granulocyte macrophage CSF . ^^^ These studies suggest a role , at least in vitro , for TNF alpha and IFN gamma in the release of CSF from subsets of T lymphocytes stimulated with PHA . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These studies suggest a role , at least in vitro , for TNF alpha and IFN gamma in the release of CSFs from cells of the mononuclear phagocytic lineage . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
All five lines proliferated and produced IL 2 , IFN gamma , and TNF in a dose dependent fashion in response to HBcAg , but not to HBV envelope Ag . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Incubation with antibodies to either IFN alpha or IFN gamma failed to affect the TNF mediated responses . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma ( 600 U / ml ) , but not TNF ( 500 U / ml ) or IFN beta ( 10 ( 3 ) U / ml ) , as single agents decreased KC TSP biosynthesis , secretion , and utilization as an attachment factor . ^^^ However , the combination of IFN gamma ( 600 U / ml ) and TNF ( 500 U / ml ) inhibited TSP mRNA production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Therefore , IL 1 , as well as IFN gamma , tumor necrosis factor ( TNF ) , G CSF , and GM CSF , were evaluated as potential therapeutic agents in irradiated C3H HeN mice . ^^^ Significant therapeutic effects were also achieved with a single dose of IFN gamma or of TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These results suggest that the presence of cytokines , such as TNF alpha and IFN alpha in synovial fluid may be associated with tissue changes observed in rheumatoid joint disease and thus contribute to the pathology of the arthritis , but support evidence for the minimal role likely to be played by IFN gamma in the joint pathology of rheumatoid arthritis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1 , IL 4 , and TNF had little or no stimulating activity for ADCC alone or with M CSF , and the other hemopoietic growth factors IL 3 and GM CSF did not promote this effector function alone or with IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of major histocompatibility complex ( MHC ) class 1 antigens was induced in eight out of nine freshly prepared tumor cell suspensions by exposure to interferon ( IFN gamma ) and tumor necrosis factor ( TNF alpha ) in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A functioning rat thyroid cell line ( FRTL 5 ) was used to study interactions of TSH with interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^ When combined , IFN gamma and TNF alpha synergized in inhibiting TSH stimulated growth . ^^^ By itself TNF alpha inhibited stimulation of 1 uptake by TSH , but augmented the enhancement seen with IFN gamma . ^^^ A reverse profile ( stimulation by IFN gamma , inhibition by TNF alpha , and stimulation by the combination ) was seen for 1 uptake , with CS increasingly diminishing all values . ^^^ TSH stimulation of growth was progressively effective with increments of CS in the medium , but consistently there was inhibition that was greater with IFN gamma than with TNF alpha and was almost total with the combined cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A synergistic increase in the cytotoxic effects of recombinant human tumor necrosis factor ( TNF alpha ) , interferons ( IFN alpha , IFN beta , and IFN gamma ) and heat stress was demonstrated in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumour necrosis factor alpha ( TNF alpha ) , interleukin 1 ( IL 1 ) beta , IL 2 , interferon ( IFN ) and IFN gamma were assayed in 33 patients with polyarteritis nodosa ( PAN ) or Churg and Strauss angiitis ( CSA ) , and three with Wegener granulomatosis ( WG ) . ^^^ In patients with WG , serum IFN alpha and IL 2 levels were also elevated , whereas IL 1 beta , IFN gamma and TNF alpha levels remained within normal limits . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The production of interferons ( IFNs ) , IFN gamma , tumor necrosis factors ( TNFs ) and TNF alpha ( TNF alpha ) by peripheral blood mononuclear cells ( PBMNCs ) of untransfused and transfused , but otherwise untreated patients with severe aplastic anemia ( SAA ) was determined using bioassays and immunoassays . ^^^ We conclude that overproduction of IFN gamma and TNF alpha by lectin stimulated PBMNCs is an intrinsic abnormality of SAA unrelated to blood transfusions . ^^^ Normalization of production of IFN gamma and TNF alpha accompanying a clinical response to cyclosporin A may cautiously be taken as further evidence suggesting a pathogenetic role of cytokine overproduction in SAA . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The accumulation of i . v . injected 111In labelled peritoneal exudate lymphocytes in skin sites of rats injected with KLH to induce DTH reactions , LPS , poly 1 : C and the cytokines , IFN gamma , IFN alpha / beta and tumor necrosis factor was determined . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The binding capacity of the endothelial cell ( EC ) for lymphocytes and monocytes is increased by IFN gamma , IL 1 , TNF alpha , and TNF beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) have potent effects on bone resorption and collagen synthesis in cultured rat long bones . ^^^ Since the effects of TNF alpha and IFN gamma may result from interaction with multiple cell types , we studied the effects of these cytokines on the synthesis of DNA and collagen in one cell type with osteoblast phenotype , cloned rat osteosarcoma cells ( ROS 17 / 2 . 8 ) . ^^^ Coincubation with TNF alpha and IFN gamma inhibited DNA synthesis more than maximal doses of either cytokine alone . ^^^ This enhanced inhibitory effect was due to the induction of a response to TNF alpha by IFN gamma , since preexposure of cells to IFN gamma for 24 h , followed by incubation with TNF alpha alone for an additional 48 h , also resulted in increased inhibition of DNA synthesis . ^^^ Preexposure to TNF alpha for 24 h , followed by IFN gamma alone , did not increase the inhibition of DNA synthesis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The mechanism of TNF induced neutrophilia was investigated by modulating the magnitude of both the first and second peaks of neutrophilia by priming of rats with daily injections of IFN gamma for 2 days prior to administration of TNF . ^^^ The first peak of neutrophilia in IFN gamma primed TNF treated rats was decreased in comparison to TNF alone treated rats because of the well known neutropenic and myelosuppressive effect of IFN gamma , which resulted in a decrease in the number of neutrophils that could be recruited to cause neutrophilia . ( ABSTRACT TRUNCATED AT 400 WORDS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) and tumor necrosis factor ( TNF ) are lymphokines with a potent hematopoietic progenitor cell suppressive capacity . ^^^ In untreated and immunosuppressed patients with severe aplastic anemia ( SAA ) and in control individuals we measured ( a ) serum levels of IFN gamma and TNF and its production by peripheral blood mononuclear cells ( PBMNC ) ; ( b ) serum levels of neopterin , a product that reflects endogenous IFN production ; ( c ) resting and activated lymphocyte subpopulations ; and ( d ) serum levels of soluble interleukin 2 receptor ( IL 2R ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These findings suggest that IFN alpha , IFN gamma , and TNF alpha may play similar roles in enhancement of HLA A , B , C expression of hepatocytes in hepatitis and hepatoma cells . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We analyzed the proliferative response of DA 1 , DA 1 a , NFS 60 , 32DC1 , Ea 3 17 and FDCP 2 murine interleukin 3 ( mIL 3 ) sensitive cell lines to human recombinant IL 1 alpha , IL 1 beta , IL 2 , IL 4 , IL 5 , IL 6 , granulocyte CSF ( G CSF ) , macrophage CSF ( M CSF ) , granulocyte macrophage CSF ( GM CSF ) , interferon gamma ( IFN gamma ) , tumor necrosis factor ( TNF ) alpha , gibbon IL 3 , purified HILDA and mouse recombinant IL 3 , IL 4 , GM CSF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A synergistic increase in procoagulant activity was also observed with primary cultures of human umbilical cord endothelial cells incubated with recombinant human IFN gamma for 16 hours prior to endotoxin , TNF , or IL 1 stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It is not IL 1 alpha or beta , TNF alpha or beta , IL 6 or IFN gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Antibody to TNF or LT failed to block the IFN gamma mediated DNA fragmentation and antibodies to IFN gamma , TNF , and LT failed to block the DNA fragmentation observed in the cells dying as a result of having exhausted their culture media . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Acute and chronic inflammatory responses to local administration of recombinant IL 1 alpha , IL beta , TNF alpha , IL 2 and Ifn gamma in mice . ^^^ The contention that cytokines are important mediators of inflammation prompted the present studies which were designed to compare acute and chronic pathological effects of locally administered recombinant ( r ) IL 1 alpha , IL 1 beta , TNF alpha , IL 2 and Ifn gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The following recombinant cytokines , IL 2 , IL 3 , IL 4 , interferon gamma ( IFN gamma ) , IFN alpha , tumor necrosis factor alpha ( TNF alpha ) , and TNF beta , as well as thymosin fraction 5 and Escherichia coli lipopolysaccharide ( LPS ) , were not found to modify TEC proliferation but IFN gamma and TNF alpha enhanced the effects of IL 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The synthetic pentapeptide pGlu Glu Asp Cys Lys ( SPI ) was evaluated in vitro alone and in combination with recombinant human tumor necrosis factor alpha ( TNF alpha ) and / or interferon gamma ( IFN gamma ) for effects on colony formation by hematopoietic progenitor cells ( HPC ) present in low density ( LD ) , nonadherent low density T lymphocyte depleted ( NALT ) , and highly enriched sorted progenitor cells from normal human bone marrow . ^^^ SP 1 synergized with TNF alpha and / or IFN gamma to inhibit proliferation of progenitor cells using both LD or My 10 DR+ human marrow cells stimulated by recombinant human interleukin 3 ( IL 3 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor ( TNF alpha ) was compared to immune interferon ( IFN gamma ) for its ability to modulate the expression and shedding of HLA antigens , of intercellular adhesion molecule 1 ( ICAM 1 ) and of high molecular weight melanoma associated antigen ( HMW MAA ) by a panel of melanoma cell lines . ^^^ TNF alpha enhanced the expression of HLA class 1 antigens on all 7 melanoma cell lines tested , although to a lower extent than IFN gamma and the combination of IFN gamma and TNF alpha . ^^^ Interestingly , both TNF alpha and IFN gamma enhanced the expression of HLA class 2 antigens by SK MEL 93 DX 3 cells . ^^^ On the other hand only TNF alpha induced the expression of HLA class 2 antigens by MeWo cells and only IFN gamma induced such expression by MeM 50 10 cells and by SK MEL 93 DX 2 cells . ^^^ The effect of the combination of TNF alpha and IFN gamma was similar to that of the individual cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This binding is enhanced by lymphokines ( IFN gamma , TNF beta ) and monokines ( IL 1 , TNF alpha ) , secreted by perivascular inflammatory cells and acting on the EC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Thus , granulocyte / monocyte colony stimulating factor ( GM CSF ) , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and interleukin 1 ( IL 1 ) were measured in the sera and spleen cell supernatants of vaccinated mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In normal rabbits , intraocular injection of human recombinant interferon gamma ( IFN gamma ) and tumor necrosis factor ( TNF ) increased the peak latency of the cortical VEP by more than 2 ms within 3 h of injection ; equal volume injections of control substances had no effect . ^^^ Alterations in conduction induced by IFN gamma and TNF reversed within 24 h and could be reinduced by reinjection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF and IFN gamma synergized to induce both species of IL 1 mRNA and IL 1 bioactivity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Intravenous injection of rat anti mouse gamma interferon ( IFN gamma ) monoclonal antibody as well as rabbit anti mouse tumor necrosis factor ( TNF ) antibody into mice which had received a sublethal infection with Listeria monocytogenes cells resulted in acceleration of listeriosis . ^^^ Production of TNF but not of IFN gamma in the bloodstream early in infection was inhibited by administration of anti IFN gamma monoclonal antibody . ^^^ The suppressive effect of anti IFN gamma and anti TNF antibodies on antilisterial resistance was not augmented by simultaneous administration of these antibodies . ^^^ On the other hand , in the secondary infection , simultaneous administration of anti IFN gamma and anti TNF antibodies , but not of either of these antibodies alone , into L . monocytogenes immune mice resulted in high mortality and explosive multiplication of bacterial cells in the spleens and livers . ^^^ These results suggest that endogenously produced IFN gamma and TNF are both essential to the host defense against L . monocytogenes infection and that these cytokines might act by different modes between the primary infection and the secondary infection . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tryptophan degradation was enhanced in the TNF treated cells and was much further increased when the cells were treated with both TNF and IFN gamma at concentrations at which IFN gamma by itself had very little effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF sensitivity of the established cell lines derived from esophageal squamous cell carcinomas and combined effect of TNF and IFN gamma ] . ^^^ IFN gamma is known to be an inducer of TNF receptor of some kinds of tumor cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Hemin and Sn protoporphyrin stimulate TNF alpha and IFN gamma production by PBMC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The clones MeM A 16 and MeM A 18 were also shown to display differential susceptibility to modulation by immune interferon ( IFN gamma ) and / or tumor necrosis factor ( TNF alpha ) of the expression of the four types of antigens analyzed . ^^^ The increase in the expression of HLA class 1 antigens induced by IFN gamma and / or TNF alpha on the two clones was associated with an increased susceptibility to lysis by anti HLA A 2 cytotoxic T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Supernatants of IL 2 activated mononuclear cells ( MNC ) that displayed an optimal lymphokine activated killer ( LAK ) cell activity at 48 72 hr in culture were found to contain increased levels of tumour necrosis factor alpha ( TNF alpha ) , interleukin 1 alpha ( IL 1 alpha ) and interferon gamma ( IFN gamma ) when compared with supernatants from mononuclear cells cultured in the absence of IL 2 . ^^^ Optimal concentrations of IL 2 needed to induce maximum release of TNF alpha , IL 1 alpha and IFN gamma by MNC varied among different individuals . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have examined the role played by interferon gamma ( IFN gamma ) and tumour necrosis factor ( TNF alpha ) in virus clearance . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma and TNF alpha did not participate in the effect because administration of anti IFN gamma or anti TNF alpha just before administration of shosaiko to or just before infection did not abrogate the inhibitory effect of shosaiko to on the bacterial growth in the early stage of infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Post transcriptional downregulation of MHC class 1 expression in oncogene transformed cells is reverted by IFN gamma and TNF alpha . ^^^ Treatment with IFN gamma and TNF alpha caused 2 to 3 fold enhancement of H 2K and beta 2 microglobulin promoter activity , as well as a normalization ( TNF alpha treatment ) or enhancement ( IFN gamma treatment ) of H 2 membrane expression . ^^^ These data suggest that IFN gamma as well as TNF alpha can counteract downregulation of H 2 genes by interference with an oncogene induced , post transcriptional block as well as by a direct enhancement of H 2 gene transcription . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When tested as single agents or added together at very low concentrations , interleukin 1 ( IL 1 ) , tumor necrosis factor ( TNF ) , and interferon gamma ( IFN gamma ) inhibited insulin release from rat islet cell monolayer cultures during 4 day incubations ; however , this secretory function improved after the cytokines were removed . ^^^ In contrast , combinations of slightly higher concentrations of IL 1 , TNF , and IFN gamma produced irreversible inhibition of insulin release , as well as decreased cell insulin content and proportional increase in cell lysis , measured as release of 51Cr from labeled islet cell cultures . ^^^ These findings suggest that cytokine products of T lymphocytes ( IFN gamma ) and macrophage / monocytic cells ( IL 1 , TNF ) infiltrating pancreatic islets in `` autoimmune ' ' diabetes may interact synergistically to produce functional inhibition or lethal cytocidal effects on islet beta cells , possibly accounting for reversible and irreversible stages of insulin dependent diabetes . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These data suggest that T cells present in autoimmune thyroid infiltrates share a number of functions , such as high production of IFN gamma , but differ with regard to their ability to secrete TNF alpha , which is peculiar of most T cells present in the thyroid of HT patients . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Despite increased binding of CD16+ lymphocytes to TNF activated EC monolayers , prior exposure of EC to any of several IL 2 inducible cytokines including TNF alpha , IL 1 beta , and IFN gamma not only failed to render the EC more vulnerable to cytolysis but increased their resistance to LAK cells in 111Indium release cytolysis assays . ^^^ Exposure to IL 2 , IFN gamma , or TNF alpha did not increase the diffusion of the BSA through the EC coated filters , whereas LAK cells markedly increased their permeability . ^^^ Consistent with the results of the cytolysis assays , pretreatment of the EC with TNF , IL 1 , or IFN gamma diminished the LAK cell induced increase in BSA diffusion . ^^^ These results suggest that although circulating IL 2 inducible cytokines such as TNF and IFN gamma may activate EC in vivo and contribute to lymphocyte margination and lymphopenia , they may not be directly responsible for the IL 2 induced capillary leak syndrome and may actually protect EC from LAK cell mediated injury . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Clone 290 2 cells secreted lymphokines potentially inhibitory for myelopoiesis ( TNF alpha , IFN gamma ) , and their supernatants could inhibit optimally stimulated granulocyte / macrophage colony formation by normal bone marrow . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although TNF alpha was equally transcribed in each subsets , IFN gamma and serine protease HF were preferentially transcribed in the CD16bright NK cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CSIF can inhibit the production of IL 2 , IL 3 , lymphotoxin ( LT ) / TNF , IFN gamma , and granulocyte macrophage CSF ( GM CSF ) by Th 1 cells responding to antigen and APC , but Th 2 cytokine synthesis is not significantly affected . ^^^ Biochemical characterization , mAbs , and recombinant or purified cytokines showed that CSIF is distinct from IL 1 , IL 2 , IL 3 , IL 4 , IL 5 , IL 6 , IL 7 , IFN gamma , GM CSF , TGF beta , TNF , LT , and P 40 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This aberrant class 2 expression is important in the mechanism of perpetuation of the disease process , therefore we have compared the effect of interferon gamma ( IFN gamma ) and tumour necrosis factor ( TNF alpha ) on the HLA DR alpha mRNA expression of thyroid follicular cells derived from Graves ' disease ( GD ) and a non autoimmune disease , non toxic goitre ( NTG ) . ^^^ Our results indicate that TNF alpha synergises with IFN gamma in the induction of HLA class 2 mRNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A clonal analysis revealed that patients with hyper IgE syndrome had markedly lower proportions of circulating T cells able to produce IFN gamma and tumor necrosis factor alpha ( TNF alpha ) in comparison with controls . ^^^ Since IFN gamma exerts an inhibitory effect on the synthesis of IgE and both IFN gamma and TNF alpha play an important role in inflammatory reactions , we suggest that the defective production of IFN gamma may be responsible for hyperproduction of IgE and the combined defect of IFN gamma and TNF alpha may contribute to the undue susceptibility to infections seen in patients with hyper IgE syndrome . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The subclone is highly specific for IL 1 : proliferation was not observed to endotoxin , human or murine interferon gamma ( IFN gamma ) , tumor necrosis factor ( TNF ) , lymphotoxin , or granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effect of tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) on collagen metabolism by human diploid fibroblasts in confluent monolayer culture was examined . ^^^ Recombinant TNF alpha reduced collagen mRNA levels 2 fold and stimulated collagenase mRNA levels 5 fold , while recombinant IFN gamma affected only collagen mRNA levels . ^^^ The combination of TNF alpha ( 10 ng / ml ) and IFN gamma ( 100 ng / ml ) resulted in a much stronger ( about 30 fold ) reduction of collagen mRNA levels indicating that the two cytokines act synergistically . ^^^ The effect of TNF alpha and IFN gamma on collagen metabolism reported here indicates a complex interaction of different cytokines in the control of tissue remodeling that occurs during inflammation , repair , or atrophy . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The relative ability of TNF and lymphotoxin ( LT ) to inhibit the growth of five human tumor cell lines was examined both in the presence and absence of IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Two cytokines produced in the course of cellular immune reactions , IFN gamma and TNF alpha , increase the number of u PA receptors on human cultured monocytes from 14 , 000 to 64 , 000 and 30 , 000 receptors / cell , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Significant induction also was observed using gamma interferon ( IFN gamma ) alone , whereas TNF alpha alone yielded only modest induction . ^^^ Classic small cell lung cancer cell lines NCI H 146 and NCI H 209 best demonstrated synergistic HLA and beta 2 microglobulin antigen induction with IFN gamma and TNF alpha with the following dose schedule : 3 6 days of TNF alpha ( 200 units / ml ) followed by 48 h of IFN gamma ( 100 IU / ml ) . ^^^ Small cell lung cancer cell line NCI H 146 was further studied for expression of major histocompatibility complex messenger RNA using the optimal doses and sequence of addition of IFN gamma and TNF alpha as indicated above . ^^^ A significant induction with IFN gamma alone and synergistic induction with both IFN gamma and TNF alpha was quantitated for both HLA A 2 and beta 2 microglobulin transcripts using Northern blot analysis . ^^^ Incubation with relatively low subcytotoxic doses of IFN gamma and TNF alpha also resulted in a marked synergistic decrease in c myc message . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Reasoning that cytokines might regulate this AGE receptor system , we have evaluated the effect of cachectin / TNF , IL 1 , and IFN gamma on AGE protein processing . ^^^ IL 1 and IFN gamma alone did not increase AGE processing , but IFN gamma consistently enhanced cachectin / TNF induced changes in AGE receptor kinetics . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effect of TNF alpha was enhanced by recombinant human interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , we also found that ACTH 1 39 will potentiate IFN gamma ' s induction of TNF alpha from these cells . ^^^ Since previous reports showed that ACTH inhibits IFN gamma ' s activation of macrophages to a cytocidal state , our data raise questions concerning the relative role of TNF alpha in macrophage mediated cytolysis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Fourth , in the mouse model , a cytokine cascade including at least GM CSF , IL 3 and IFN gamma is required for the elevation of TNF level . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Peripheral blood mononuclear cells ( PBMC ) from 14 patients with severe atopic dermatitis ( AD ) and 11 healthy donors were tested for their capacity to produce tumour necrosis factor alpha ( TNF alpha ) after PHA stimulation and compared with their in vitro production of interferon gamma ( IFN gamma ) . ^^^ However , a significant portion of patients with AD which was defective in generating IFN gamma in vitro showed in addition significantly a decreased production of TNF alpha . ^^^ No correlation could be found between TNF alpha and neopterin production in either group , whereas there was a close overall correlation between the amount of TNF alpha and IFN gamma detectable in culture supernatants of patients and controls . ^^^ Furthermore , a significant correlation was found between TNF alpha and IFN gamma generation in vitro and serum IgE concentration in AD . ^^^ Furthermore , 3 AD patients with normal IFN gamma generation and low serum IgE concentration but suffering from eczema herpeticum formed a subgroup which showed an increased TNF alpha production in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , the secretion of tumour necrosis factor ( TNF ) was not stimulated by either antigen , and IFN gamma production was not diminished by antisera directed at TNF alpha or TNF beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) can act synergistically with IFN gamma with respect to class 2 expression on a variety of cells . ^^^ As brain cells themselves can secrete TNF like factors upon stimulation , we examined the effect of TNF alpha on IFN gamma mediated class 2 induction on astrocytes . ^^^ TNF alpha alone had no effect on class 2 expression , but did synergize with IFN gamma for enhanced expression of class 2 antigens . ^^^ Kinetic analysis of the synergistic effect indicated that optimal TNF alpha enhancement of class 2 expression was observed when astrocytes were pretreated with IFN gamma 12 24 h prior to TNF alpha addition . ^^^ A possible mechanism for the synergistic action between IFN gamma and TNF alpha may be increased TNF alpha receptor expression by IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
BGEF 2 itself did not show IL 2 activity nor IL 1 activity , and BGEF 2 activity was not detected in the following cytokines : Interferon alpha ( IFN alpha ) , interferon gamma ( IFN gamma ) , tumor necrosis factor ( TNF ) , interleukin 4 ( IL 4 ) , interleukin 5 ( IL 5 ) and interleukin 6 ( IL 6 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Additionally , hepatic cholesterol synthesis was increased 16 17 h after TNF beta , IL 1 , and IFN gamma treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Other cytokines tested like TNF alpha and beta , IFN gamma , IL 1 , IL 2 , and IL 5 did not induce IL 6 secretion when given to resting B cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The ability of TNF alpha to inhibit anabolic processes in adipocytes and preadipocytes is not unique and has been displayed , though to a lesser degree , by other cytokines such as LT , IFN gamma , and IL 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
As the commercially available BCGFlow is known to contain low concentrations of IL 2 , IFN gamma , TNF and GM CSF as impurities , it was important to exclude these as being responsible for the TRF activity . ^^^ In contrast , a combination of IL 2 ( 0 . 2 U / ml ) , IFN gamma ( 50 U / ml ) , TNF alpha ( 50 U / ml ) and TNF beta ( 100 U / ml ) did have TRF activity suggesting that B cells could be made to respond to low doses of IL 2 by the presence of other cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The other line ( TFATx ) , established from a co culture between the autologous PBL , lethally irradiated TFTx and the autologous melanoma cells TF M , is IL 2 dependent for growth , secretes IFN gamma and TNF alpha and beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although LC , IL 1 , IL 2 , IFN gamma , and the T effector circuits have been extensively studied , it is still not clear whether it is LC or keratinocyte derived IL 1 that is crucial in ACH , whether IL 1 acts primarily on T cells or the APC , whether other cytokines are involved in the circuit ( TNF , KTGF ? ) , the exact relationships between T effector , T memory , and other T helper cells , what the functions of mast cells and basophils are in the allergic reaction , and how the regulatory circuits ( including prostaglandins and eicosanoids ) affect the outcome of ACH . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These enhancing activities of mouse serum were nondialyzable , inactivated by incubation at pH 2 for 24 h , sensitive to pepsin digestion , stable at 56 degrees C for 30 min , and could not be replaced by defined cytokines such as IL 1 , IL 2 , IL 3 , IL 4 , IL 6 , IFN alpha / beta , IFN gamma , TNF alpha , CSF 1 , or GM CSF . ^^^ In contrast with marrow M phi , thioglycollate elicited peritoneal M phi , which normally release only small quantities of PGE 2 , showed over 5 fold increases in PGE 2 production following treatment with IFN gamma , IFN alpha / beta , or TNF , but not with other cytokines or mouse serum . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
BRMs used in this study were OK 432 , tumor necrosing factor ( TNF ) , whole peptide glucagon ( WPG ) , interferon ( IFN ) alpha , IFN beta , IFN gamma and interleukin 2 ( IL 2 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Macrophages ( Mphi ) and Mphi depleted ( nonadherent ) nonparenchymal cells ( NPC ) of the liver were examined for their cytotoxic potential against tumor cells , production of tumor necrosis factor ( TNF ) , and release of prostaglandins ( PG ) following stimulation by lipopolysaccharide ( LPS ) , interferon gamma ( IFN gamma ) , and zymosan . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) enhanced TNF alpha synthesis in cultures from either SLE patients or controls stimulated by concanavalin A ( Con A ) alone , but depressed the high production of TNF alpha by normal PBMC stimulated with Con A plus PMA . ^^^ IFN gamma enhanced TNF alpha production in response to Con A plus PMA in 2 of 3 SLE patients . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Among recombinant mouse IFN gamma , human IL 1 alpha , human IL 2 , mouse granulocyte macrophage colony stimulating factor and human TNF alpha , only human TNF alpha suppressed insulitis and significantly ( P less than 0 . 001 ) inhibited development of diabetes . ^^^ NOD mice were the lowest producers of the mRNA of TNF and serum TNF on stimulation with OK 432 or with IFN gamma plus LPS , compared with C57BL / 6 , C3H / He , and Balb / c mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Myelinated cultures of embryonic SJL / J mouse spinal cord tissue have been tested for their ability to express class 2 major histocompatibility complex ( MHC ) ( Ia ) molecules in the presence of two recombinant cytokines , mouse interferon gamma ( IFN gamma ) and human tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
P 48 has been purified to homogeneity and is distinct from TNF alpha TNF beta , IFN gamma , IL 6 , and macrophage CSF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Together , TNF and interferon gamma ( IFN gamma ) additively and synergistically caused increases in these activities as well as the expression of HLA DR and the monocyte antigens LeuM 3 ( CDw 14 ) and OKM 1 ( CD 11 ) . ^^^ The potentiating actions of D 3 of IFN gamma on the TNF effect were maximal when the two agents were present together throughout the incubation , and pretreatment with TNF augmented the subsequent response to D 3 , but not IFN gamma . ^^^ HL 60 TNF receptors were not changed by a three day treatment with IFN gamma or D 3 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The combination of retinoic acid or tumor necrosis factor alpha ( TNF alpha ) with interferon gamma ( IFN gamma ) resulted in a synergistic amplification of the anti proliferative effect of IFN gamma on cultured breast cancer cells . ^^^ On the other hand , only TNF alpha and not retinoic acid was able to increase the IFN gamma induced expression of HLA DR on the cell surface . ^^^ Data obtained in this study demonstrate a different pattern of action between retinoic acid and TNF alpha regarding their synergism in combination with IFN gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We report evidence that FcR ( CD 16 ) on human NK cells are signal transducing molecules that , upon ligand binding , induce transcription of genes encoding surface activation molecules [ IL 2 R ( CD 25 ) ] and cytokines ( IFN gamma and TNF ) relevant to NK cell biology and functions . ^^^ Upon 18 h of stimulation , NK cells express Tac , TfR , and 4F2 antigens and produce IFN gamma and TNF ; both effects are synergistically enhanced in the presence of rIL 2 , which is itself ineffective . ^^^ Treatment of NK cells with FcR ( CD 16 ) ligands induces accumulation of mRNA for IFN gamma and TNF and , to a lesser extent , IL 2 R with fast kinetics also in the absence of de novo protein synthesis . rIL 2 and FcR ( CD 16 ) ligands synergize to induce mRNA accumulation . mRNA accumulation and transcription of TNF and IFN gamma genes induced by FcR ( CD 16 ) ligands are greater than those induced by rIL 2 , and the reverse is true for IL 2 R . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha alone or in combination with IFN gamma differentially affects the proliferation and differentiation of the human leukemic cell line U 937 and two derivatives C 27 and G 3 . ^^^ All three cell lines express similar numbers of functional , high affinity receptors for both TNF alpha and IFN gamma . ^^^ In C 27 and G 3 cells , TNF alpha as well as IFN gamma induced changes in steady state levels of specific mRNA , which appear to be associated with TNF alpha and IFN gamma diverse effects on cell growth and differentiation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor , alone or in combination with IL 2 , but not IFN gamma , is associated with macrophage killing of Mycobacterium avium complex . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Monocyte cytokines , neither IL 1 , IFN gamma , TNF , nor their combination were able to substitute for monocytes in the induction culture . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The receptor did not recognize IL 2 , IFN gamma , tumor necrosis factor alpha , a functionally related monokine , or bovine acidic fibroblast growth factor , a structurally related mediator . ^^^ Labeling of these bands was blocked by IL 1 beta and IL 1 alpha but not by IL 2 , IFN gamma , or tumor necrosis factor alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor ( TNF ) inhibited IFN gamma induced Fc receptor expression on human monocytes but was much less potent than comparable amounts of IL 1 . ^^^ In fact , IL 1 or TNF led to an enhancement in IFN gamma induced Fc receptor expression on THP 1 cells . ^^^ These results indicate that LPS can inhibit IFN gamma induced Fc receptor expression on human monocytes and that IL 1 and TNF may mediate these effects of LPS . ^^^ Tumor necrosis factor ( TNF ) inhibited IFN gamma induced Fc receptor expression on human monocytes but was much less potent than comparable amounts of IL 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cells were unresponsive to IL 1 , IL 4 , tumor necrosis factor alpha ( TNF alpha ) , interferon alpha ( IFN alpha ) , IFN gamma , phytohemagglutinin and concanavalin A . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The binding of labelled TNF alpha to these cells can be inhibited by both TNF alpha and TNF beta but not by gamma interferon ( IFN gamma ) . ^^^ However , preincubation of cells with IFN gamma increases the total number of TNF receptors two to threefold without any significant change in the affinity constant . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Incubation of several human tumor cell lines with human interferon gamma ( IFN gamma ) increased the specific binding of subsequently added 125I labeled recombinant human tumor necrosis factor ( TNF ) . ^^^ A similar increase in TNF binding was seen in murine L 929 cells after incubation with murine IFN gamma , but not after incubation with human IFN gamma . ^^^ Increased TNF binding to cells incubated with IFN gamma was due to an increase in the number of TNF receptors , with no demonstrable change in binding affinity . ^^^ In one out of two human cell lines tested , IFN alpha and IFN beta also produced increased TNF binding , albeit with a lower efficacy than IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Incubation of HeLa cell cultures with human interferon gamma ( IFN gamma ) increased the binding of radioiodinated human tumor necrosis factor ( TNF ) to specific cell surface receptors ( TNF R ) . ^^^ IFN gamma also produced a proportionate increase in receptor mediated endocytosis of TNF . ^^^ TNF R expression was significantly increased after 6 h of exposure to IFN gamma ( 100 units / ml ) , and it remained elevated in the continuous presence of IFN gamma for at least 20 h . ^^^ Incubation of cells with IFN gamma in the presence of cycloheximide , followed by treatment with actinomycin D and reversal of the inhibition of protein synthesis , also resulted in increased TNF R expression as compared to cultures subjected to the same treatments in the absence of IFN gamma . ^^^ These results suggest that IFN gamma can directly stimulate accumulation of the mRNA for TNF R and that TNF R is among the cellular proteins whose synthesis is increased by IFN gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this article , the antitumor effects of rH TNF against human and murine tumors , the mechanism of its action and the synergistic effects of rH TNF in combination with IFN gamma or with cyclophosphamide are reviewed . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1 and LPS slightly suppressed factor H mRNA , while TNF had no effect . mRNA for factor B is also detectable in IFN gamma stimulated cells , but messengers for C1q , C4bp , and CR 3 beta chain were not found . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , IFN gamma increased 125I TNF binding in both cell lines . ^^^ Both IFN alpha and IFN beta exerted an antagonistic effect on IFN gamma induced stimulation of TNF receptor expression in HT 29 cells , but did not inhibit TNF receptor induction by IFN gamma in HeLa cells . ^^^ IFN gamma and , to a lesser extent , IFN beta were synergistic with TNF in producing cytotoxic / cytostatic activity in HT 29 cells . ^^^ Despite the inhibitory effect of IFN beta on the IFN gamma induced stimulation of TNF receptor expression , IFN beta did not inhibit the synergistic enhancement of TNF cytotoxicity by IFN gamma in HT 29 cells . ^^^ Differential effects of type 1 IFN and IFN gamma on the binding of tumor necrosis factor to receptors in two human cell lines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In a priori HLA negative cells , a strong TNF alpha mediated enhancement of the Interferon gamma ( IFN gamma ) induced expression of HLA genes was revealed by Northern blot and immunofluorescence analysis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Comparison of TNF receptor expression with that of high affinity interleukin 2 ( IL 2 ) and interferon gamma ( IFN gamma ) receptors , respectively , revealed similarities to IL 2 receptor expression with respect to kinetics of induction . ^^^ Moreover , TNF alpha was effective as a co stimulator of IL 2 dependent IFN gamma production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this article , the antitumor effects of rH TNF against human and murine tumors , the mechanism of its action and the synergistic effects of rH TNF in combination with IFN gamma , various anticancer drugs or with hyperthermia are reviewed . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The addition of either IL 1 or IFN gamma to TNF alpha resulted in a substantial further increase in DNA synthesis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A variety of agents capable of inducing tumoricidal activity have been described , including bacterial LPS , IFN gamma , IL 1 , IL 2 , TNF , and GM CSF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Use of antibodies for TNF alpha , IFN gamma , or TNF beta as well as measurement of TNF and IFN titers suggested that the suppressor molecule ( s ) released from monocytes were acting with TNF alpha and those released from PHA stimulated T lymphocytes were acting with IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma strongly enhanced the TNF alpha mediated growth inhibition of 3 sensitive cell lines , but had no effect on 7 other leukemic cell lines with little or no TNF sensitivity . ^^^ No correlation was found between this enhancement of TNF sensitivity and the IFN gamma mediated increase in TNF cell membrane receptors , suggesting that IFN gamma predominantly exerts its synergistic effect distal to TNF binding . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , remarkable synergism between TNF and interferon gamma ( IFN gamma ) was observed in a large number of human cell lines , especially breast , cervix and colon carcinomas . ^^^ Some other human cell lines , not sensitive to TNF alone , became highly sensitive when IFN gamma was present as well . ^^^ We could not demonstrate a synergism between TNF and IFN gamma on any of the lymphoma / leukemia cell lines tested . ^^^ All normal human , non transformed diploid cell lines were insensitive to TNF even in the presence of IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This species specificity was studied for the TNF activity alone or in synergism with IFN gamma . ^^^ Also , synergism with IFN gamma seemed more effective when the target cell was treated with homologous TNF . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have used the quantitative binding of murine monoclonal antibodies to the surface of cultured human umbilical vein endothelial ( HUVE ) cells to study the responses of HUVE cells to three different immune mediators : interleukin 1 ( IL 1 ) , tumor necrosis factor ( TNF ) , and immune interferon ( IFN gamma ) . ^^^ Antibody RR1 / 1 , reactive with intercellular adhesion molecule 1 , binds to unstimulated HUVE cells , but binding is rapidly increased ( plateau 24 hr ) after stimulation by IL 1 or TNF and slowly increased ( over several days ) by IFN gamma . ^^^ Antibody W6 / 32 , reactive with HLA A , B antigens , binds to unstimulated HUVE cells and shows gradually progressive increases ( over several days ) in binding upon treatment with IFN gamma or TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study was designed to determine whether anti fungal activity in human polymorphonuclear neutrophils ( PMN ) might be under the regulation of cytokines such as tumor necrosis factor ( TNF ) and interferon gamma ( IFN gamma ) . ^^^ TNF and IFN gamma added to the PMN / candida cultures additionally enhanced PMN to inhibit candida growth . ^^^ Concentrations of TNF and IFN gamma below the level that enhanced PMN function when added together to PMN acted synergistically to significantly enhance their anti fungal activity . ^^^ Therefore , TNF and IFN gamma which are active on lymphoid cells , also appear to have the ability to directly activate PMN , and the synergistic action of the two cytokines at low doses that may be below the toxic range may prove to be of clinical importance in protection of immunocompromised host against opportunistic infections . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These data show that the cytotoxins TNF and LT mediate complex effects on cells of the myelomonocytic lineage and , in synergy with IFN gamma , can fully induce immature myeloid cells to differentiate into cells with phenotypic , functional , and proliferative characteristics of terminally differentiated myelomonocytic cells . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma permits the endotoxin induced production of cachectin by C3H / HeJ ( endotoxin resistant ) macrophages , apparently by facilitating endotoxin induced cachectin biosynthesis at both transcriptional and posttranscriptional levels . ^^^ IFN gamma can not induce cachectin biosynthesis by itself , nor does it markedly enhance cachectin production by endotoxin induced peritoneal macrophages obtained from endotoxin responsive mice . ^^^ Elucidation of the precise mechanism through which IFN gamma influences cachectin biosynthesis may permit a better understanding of the molecular events that follow endotoxin induced activation of macrophages . ^^^ Moreover , the permissive effect of IFN gamma on cachectin biosynthesis might elicit enhanced endotoxin sensitivity in vivo . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effect of immune interferon ( IFN gamma ) and recombinant tumor necrosis factor ( rTNF alpha ) on cellular differentiation was investigated in human promyelocytic leukemia cell line HL 60 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Exposure of mouse resident and thioglycollate elicited peritoneal macrophages to IFN gamma leads to a marked increase in the TNF alpha ( tumor necrosis factor / cachectin ) , IL 1 and u PA ( urokinase type plasminogen activator ) mRNA levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effect of tumor necrosis factor ( TNF ) and of interferon gamma ( IFN gamma ) on c myc expression was investigated in HeLa cells , which respond to these cytokines by a specific arrest in the G0 / G1 phase of the cell cycle . ^^^ While the reduction of c myc messenger RNA by IFN gamma was dependent on new protein synthesis , the inhibitory effect of TNF on c myc messenger RNA was direct and was not abrogated by cycloheximide . ^^^ The differential effect of the protein synthesis inhibitor and the cooperative inhibitory effects of the two cytokines when added together suggest that IFN gamma and TNF reduce c myc transcription through different molecular mechanisms . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Recombinant human IFN alpha or IFN gamma in the presence or absence of IL 1 had no effect on rabbit chondrocytes , whereas recombinant human tumor necrosis factor decreased plasminogen activator but had no effect on prostaglandin or metalloproteinase production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vivo anti tumour activity of recombinant human and murine TNF , alone and in combination with murine IFN gamma , on a syngeneic murine melanoma . ^^^ Although TNF is not species specific in vitro , the effects of treatment with human and murine rTNF in vivo are different : with murine rTNF , the synergism with rIFN gamma is relatively less clear , the addition of IFN gamma is not necessary to induce regression , toxicity is more pronounced and additional mechanisms of tumoritoxicity could be involved . ^^^ These results give further evidence in favour of a potential clinical use of TNF in combination therapy , e . g . with IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The combined effects of TNF and interferon gamma ( IFN gamma ) were additive on the PLC / PRF / 5 cells as shown by statistical analyses . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytotoxins tumour necrosis factor ( TNF ) and lymphotoxin ( LT ) synergize with IFN gamma in a number of activities . ^^^ We report here that IFN gamma in combination with either TNF or LT induces islet cell class 2 expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The results obtained using immune interferon ( IFN gamma ) in combination with the two inducers suggest that the mechanism of action of TNF and IFN gamma are possibly different . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of human recombinant tumor necrosis factor ( TNF ) and interferon gamma ( IFN gamma ) in damage of liposome membranes were examined to elucidate the molecular mechanism of their antiproliferative actions on tumor cells . ^^^ The extent of membrane damage was assayed by measuring the rate of release of the fluorescent dye calcein encapsulated in the liposomes at different pH values in the presence of TNF and / or IFN gamma . ^^^ In contrast , IFN gamma induced very slow leakage of dye although it bound almost completely to the membranes , suggesting that it causes much less membrane damage than TNF . ^^^ In the presence of both TNF and IFN gamma at pH 5 , the maximal leakage rate was twice the sum of the rates with the two proteins individually , and the rate depended on the TNF / IFN gamma ratio , indicating synergistic effects of TNF and IFN gamma in induction of membrane damage . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of HT 29 cells with recombinant human IFN gamma increased their susceptibility to both TNF cytotoxicity and monocyte killing . ^^^ In addition , MAb to TNF inhibited monocyte killing in HT 29 cells sensitized by incubation with IFN gamma . ^^^ Our data show that TNF is an important mediator of the cytotoxicity of human monocytes for tumor cells and that IFN gamma can increase monocyte cytotoxicity by sensitizing target cells to the lytic action of TNF . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Appearance of the monocytic phenotype was manifested within 24 hr of IFN gamma exposure by : increased nitroblue tetrazolium reduction ; increased cell surface expression of the HLA DR , Mo 1 , and MY 4 antigens ; and induction of transcripts for the second component of complement ( C 2 ) and tumor necrosis factor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neither depletion of T cells nor addition of neutralizing Abs to IFN gamma or TNF alpha prevented M1 / 70 mediated macrophage activation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These studies were designed to examine the immunodulatory and immunotherapeutic properties of recombinant murine interferon gamma ( rM IFN gamma ) and recombinant human tumor necrosis factor ( rH TNF ) . ^^^ The therapeutic effects of rH TNF were as great in these in vivo systems as those of rM IFN gamma . ^^^ Furthermore , rH TNF had additive therapeutic activity when administered in conjunction with suboptimal doses of rM IFN gamma . ^^^ Unlike rM IFN gamma , rH TNF did not activate natural killer cells in vivo or in vitro but did augment in vivo and in vitro macrophage tumoricidal activity . ^^^ In summary , rH TNF and rM IFN gamma have therapeutic activity for metastatic disease as individual agents and additive therapeutic activity when used in combination . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We assessed the antiproliferative effects of tumor necrosis factor ( TNF alpha ) and gamma interferon ( IFN gamma ) alone and in combination , on nine human colon carcinoma cell lines . ^^^ A synergistic antiproliferative effect occurred in eight of the nine cell lines treated with a combination of TNF alpha and IFN gamma . ^^^ In two cell lines with an induced resistance to mitomycin C , an increase in resistance to combined TNF alpha and IFN gamma treatment correlated with an increasing resistance to mitomycin C . ^^^ Combinations of TNF alpha / IFN gamma enhanced the dose response activity of TNF alpha in three cell lines ( P less than or equal to 0 . 09 ) and decreased the dose response activity of IFN gamma in another three ( P less than or equal to 0 . 02 ) . ^^^ Colony forming experiments on HCT 116 cells demonstrated a reduction in the number of 250 micron colonies in the IFN gamma / TNF alpha treatment groups when compared to controls , indicating that combined treatment had a cytotoxic effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Recently , it has been shown that the cytokines interleukin 1 ( IL 1 ) , tumor necrosis factor alpha ( TNF alpha ) , and TNF beta [ also called lymphotoxin ( LT ) ] produced by immune cells all stimulate osteoclast activity , whereas the lymphokine interferon gamma ( IFN gamma ) inhibits osteoclast activity stimulated by these agents . ^^^ In contrast to its action on bone resorption , where it opposed the resorptive effects of IL 1 , TNF , and LT , IFN gamma inhibited bone collagen synthesis and had an additive effect with TNF and LT when bones were exposed to both . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pretreatment of macrophages with IFN gamma resulted in the release of higher amounts of TNF on subsequent induction with either LPS or lipid 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , when IFN gamma was combined with TNF , induction of NBT reducing activity was remarkably increased . ^^^ Treatment with both TNF and IFN gamma synergistically enhanced morphological changes , growth inhibition and activity of Fc receptors , and NBT reduction in ML 1 cells , but not phagocytic activity . ^^^ The results indicate that combinations of TNF and IFN gamma act synergistically in the induction of differentiation of human myeloblastic ML 1 cells . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 2 receptor positive , but not IL 2 receptor negative , T cells released large amounts ( 190 + / 60 U / 10 ( 6 ) cells 10 ml 1 ) of IFN gamma but only low amounts of tumor necrosis factor beta ( less than 10 U / ml ) in response to IL 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the ability of cyclosporin A ( CsA ) and transforming growth factor beta ( TGF beta ) to modulate the production of TNF alpha and TNF beta and IFN gamma by unseparated , nonadherent , and adherent PBMC . ^^^ Treatment of unseparated PBMC with CsA resulted in a significant dose dependent inhibition of all three cytokines ranging from greater than 90 % inhibition for IFN gamma and TNF beta , to approximately 70 % for TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of recombinant human tumor necrosis factor ( TNF ) , lymphotoxin ( LT ) , and interferon gamma ( IFN gamma ) on the growth of human hemopoietic progenitor cells in clonal culture have been examined . ^^^ A suppressive effect of TNF , LT , and IFN gamma on the development of granulocyte , macrophage , and mixed granulocyte / macrophage colonies was shown . ^^^ In the presence of a monoclonal antibody to TNF , or polyclonal antibodies to either LT or IFN gamma , the inhibitory effect of the molecule to which the antibody was directed was abrogated . ^^^ These findings suggest that progenitor cells responsive to G CSF or GM CSF have different sensitivities to the effects of TNF , LT , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The addition of interferon gamma ( IFN gamma ) or preexposure to IFN gamma and then stimulation with TNF did also not result in IL 1 secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Preclinical approaches to the treatment of metastatic disease : therapeutic properties of rH TNF , rM IFN gamma , and rH IL 2 . ^^^ The present studies were undertaken to examine the immunotherapeutic properties of recombinant murine interferon gamma ( rM IFN gamma ) , recombinant human tumour necrosis factor ( rH TNF ) , and recombinant human interleukin 2 ( rH IL 2 ) in preclinical metastasis models . ^^^ Furthermore , rH TNF has additive therapeutic activity when administered in conjunction with suboptimal doses of rM IFN gamma . ^^^ Unfortunately , the additive therapeutic activity of rM IFN gamma and rH TNF is also associated with increased toxicity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , microglial cells exposed to IFN gamma and endotoxin developed tumor cell cytotoxicity and produced tumor necrosis factor alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The secretion of tumor necrosis factor ( TNF ) by macrophages is initiated by lipopolysaccharide ( LPS ) ; considerable evidence indicates that such secretion can be potentiated by interferon gamma ( IFN gamma ) . ^^^ The present studies show that accumulation of mRNA for tumor necrosis factor , which represents an important regulatory focus for controlling secretion of TNF , is enhanced by physiologic doses of IFN gamma ( 20 units / ml of purified recombinant IFN gamma ) . mRNA for TNF induced by LPS , which was maximal 2 hr after LPS was applied to the cells , was enhanced 5 to 8 fold by IFN gamma as determined by Northern blot analysis . ^^^ Interferon did not change the kinetics of accumulation but did change the dose effects of LPS in that increasing amounts of LPS led to increasing amounts of TNF mRNA in IFN gamma treated macrophages . ^^^ IFN gamma itself , however , did not induce expression of TNF mRNA . ^^^ These studies document that IFN gamma potentiates the cytoplasmic accumulation of mRNA for TNF induced in murine peritoneal macrophages by LPS . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In order to investigate differences in control mechanisms between embryonic and adult hemopoiesis , we have studied the sensitivity of human embryonic progenitors ( 5 8 weeks postconception ) to either positive ( erythropoietin ( Ep ) , granulocyte macrophage colony stimulating factor ( GM CSF ) and insulin like growth factor 1 ( IGF 1 ] or negative ( tumor necrosis factor ( TNF ) and interferon gamma ( IFN gamma ] in vitro regulators of adult hemopoietic differentiation . ^^^ Formation of granulocyte macrophage colonies from embryonic progenitors was induced by GM CSF but inhibited by TNF and IFN gamma . ^^^ Early erythroid progenitors resemble adult erythroid burst forming cells ( BFU E ) in their sensitivity to Ep and TNF but differ in their lack of response to GM CSF or other adult sources of burst promoting activity , and absence of inhibition by IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Since secretion of IFN gamma and conversion of circulating 25 ( OH ) vitamin D 3 to the active dihydroxy metabolite by interferon activated macrophages probably occur in tuberculosis , we suggest that triggering of TNF release from the resulting activated macrophage populations might explain much of the weight loss and tissue damage that characterize this disease . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Incubation of fibroblasts with IFN gamma produced some reduction in their accessory activity and the inhibitory effect of TNF was further enhanced in the presence of IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Application of the LT IRMA in combination with previously developed human gamma interferon ( IFN gamma ) and human TNF specific IRMA ( Crane et al . , 1985 ; Meager et al . , 1987 ) permitted independent estimations of these three substances to be carried out in parallel . ^^^ By these means , it was found that RPMI 1788 produced both LT and TNF , but not IFN gamma . ^^^ Co production of LT , TNF and IFN gamma was a common finding , even occurring in alloantigen specific T helper cell clones . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Induction of TNF mRNA in T cells from healthy individuals displayed a two signal requirement ( phorbol myristate 13 acetate and phytohemagglutinin or OKT 3 and phorbol myristate 13 acetate ) , similar to that described for the induction of the T cell lymphokines IL 2 and interferon gamma ( IFN gamma ) . ^^^ The evidence suggests that T cells can produce TNF and have the potential to deliver by themselves the dual and synergistic signals of TNF / LT and IFN gamma to target cells , a process which may be of importance in the pathogenesis of human autoimmunity . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When tested alone , murine ( rat and mouse ) interferon gamma ( mIFN gamma ) produced a small dose dependent lysis of islet cells ; human IFN gamma , mouse IFN alpha / beta , interleukins 1 and 2 ( IL 1 and IL 2 ) , tumor necrosis factor ( TNF ) , and lymphotoxin ( LT ) were inactive . ^^^ These results indicate that the cytokine products of mononuclear cells of the immune system , IFN gamma , TNF , LT , and IL 1 have strong synergistic cytotoxic effects on islet cells and therefore may act as direct chemical mediators of islet beta cell destruction in type 1 ( insulin dependent ) diabetes . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both IFN alpha and IFN gamma exhibited antiviral activity , whereas both IL 1 and TNF stimulated fibroblast growth . ^^^ The greatest similarities in polypeptide induction occur between IFN alpha and IFN gamma and between the IL 1s and TNF . ^^^ However , polypeptides were also induced in common by IFN alpha and TNF , IFN gamma and IL 1 , and IFN gamma and TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of class 1 and class 2 major histocompatibility complex ( MHC ) molecules on the surface of cultured human umbilical vein endothelial cells ( HUVEC ) incubated with alpha tumor necrosis factor ( TNF ) or interferon gamma ( IFN gamma ) was determined by fluorescence flow cytometry . ^^^ TNF in combination with IFN gamma enhances HUVEC expression of HLA alpha , B2m , and HLA DQ greater than that observed with either mediator alone . ^^^ TNF and IFN gamma reduced HUVEC expression of HLA DR below the level observed with IFN gamma alone . ^^^ Indomethacin ( INDO ) does not effect expression of HUVEC class 1 or class 2 MHC molecules induced by TNF , IFN gamma , or the combination of these mediators . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neither TNF alpha nor TNF beta could induce tumor cytotoxicity in IFN gamma primed macrophages , indicating that TNF can not replace LPS as a triggering signal of activation . ^^^ In LPS resistant C3H / HeJ macrophages , which were unresponsive to IFN gamma plus LPS , a supplementation with TNF fully restored activation to tumor cytotoxicity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Ag expression could be induced by IFN gamma but not by IFN alpha , IFN beta , or TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we have investigated the effects of tumour necrosis factor ( TNF ) , interferon gamma ( IFN gamma ) and interleukin 1 ( IL 1 ) on rat thyroid cells , using the continuously growing , differentiated FRTL 5 cell line . ^^^ No effect on cell growth was found with any cytokine alone , but the combination of TNF and IFN gamma was cytostatic . ^^^ These cells were not killed in a 18 hr assay for cytotoxicity , but prolonged incubation ( 4 days ) with TNF and IFN gamma produced cell detachment and death . ^^^ Normal rat thyroid cells in primary culture were also susceptible to the combination of TNF and IFN gamma for 4 days , but not 18 hr . ^^^ In addition , IFN gamma treatment induced class 2 ( Ia ) antigens on thyroid cells , and this was enhanced by TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Measurement of Fru ( 2 , 6 ) P 2 proved to be the most sensitive parameter for an assessment of glycolysis : IL 1 alpha , IL 1 beta and IFN gamma all produced a 3 6 fold increase in this metabolite whereas tumour necrosis factor ( TNF alpha ) was far less effective . ^^^ Prostaglandin E production was stimulated predominantly by IL 1 alpha and IL 1 beta rather than by IFN gamma or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We studied the effect of tumor necrosis factor ( TNF ) and gamma interferon ( IFN gamma ) , alone and in combination , on the expression of chemotactic peptide receptors , stimulus induced actin polymerization , hydrogen peroxide production ( H2O2 ) , and expression of nonspecific esterase ( NSE ) positivity in human promyelocytic leukemic cell line HL 60 . ^^^ IFN gamma was less potent in inducing all the parameters studied but exerted a positive cooperative effect when combined with TNF . ^^^ IFN gamma at high concentrations induced chemotactic peptide receptors comparable in magnitude to that seen with TNF but failed to prime these cells to undergo actin polymerization in response to FMLP or PMA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma and TNF were synergistic . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) do not induce monocyte procoagulant activity , although they both activate monocytes in several respects . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , an additive effect of the TNF alpha and IFN gamma has been observed . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Antiproliferative effects exerted by recombinant human tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) on human pancreatic tumor cell lines . ^^^ Modulation of the growth of human pancreatic cancer cell lines in vitro by recombinant human tumor necrosis factor alpha ( TNF alpha ) and recombinant human interferon gamma ( IFN gamma ) was investigated . ^^^ When administered together with IFN gamma TNF alpha showed enhanced antiproliferative effects on a subset of the pancreatic cancer cell lines tested , including those found to be insensitive to treatment with TNF alpha alone . ^^^ Thus the antiproliferative effect achieved by a combined treatment with TNF alpha and IFN gamma exceeded that observed with either drug alone in seven of nine pancreatic cancer cell lines . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The macrophage activating factor gamma interferon ( IFN gamma ) was also shown to induce , in the absence of endotoxin , TNF alpha secretion by monocytes in a dose dependent manner . ^^^ The induction of TNF alpha production by supernatants of proliferating T cells could largely be abrogated by passaging the supernatants on an anti IFN gamma column before adding them to the monocytes . ^^^ It is therefore concluded from this study that the production of TNF alpha by monocytes can be induced by proliferating T cells and that this induction can largely be attributed to the T cell lymphokine IFN gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon ( IFN ) is able to enhance the synthesis of both IL 1 and TNF in monocytes macrophages and , in the case of IFN gamma , to induce the expression of HLA class 2 antigens in synovial cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Supernatants from PBM cultured overnight with or without IFN gamma were tested in a bioassay measuring cytotoxicity against U 937 target cells as well as in an RIA specific for TNF . ^^^ Treatment with IFN gamma augmented the level of TNF production in both AIDS and normal PBM . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In control cultures , only astrocytes expressed MHC class 1 , but after incubation with either IFN gamma or TNF alpha oligodendrocytes acquired class 1 expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effect of the i . v . administration of endotoxin ( LPS ) , diphosphoryl lipid A , and the non toxic derivative monophosphoryl lipid A ( MPL ) , on the production of serum cachectin ( TNF ) , IFN gamma , and the appearance of endotoxin shock have been measured in mice primed with Listeria monocytogenes . ^^^ Induction of tumor necrosis factor , IFN gamma , and acute lethality in mice by toxic and non toxic forms of lipid A . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Isolated human and mouse pancreatic islet cells and the rat insulinoma cell line RIN m5F were used to examine the ability of recombinant interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) to regulate the expression of the class 1 and class 2 major histocompatibility ( MHC ) surface proteins and mRNA in beta cells . ^^^ The order of potency of the cytokines on class 1 MHC expression was TNF alpha plus IFN gamma greater than or equal to IFN gamma greater than or equal to TNF alpha . ^^^ While IFN gamma or TNF alpha alone were without effect , in combination they were found to induce class 2 MHC proteins on 30 40 % of human or murine beta cells . ^^^ In contrast , IFN gamma plus TNF alpha did not induce detectable class 2 MHC proteins or mRNA in the RIN m5F cells . ^^^ These findings indicate that 1 ) TNF alpha , in addition to IFN gamma , upregulates the expression of beta cell class 1 MHC proteins and mRNA , and 2 ) more than one signal is required for the induction of class 2 MHC proteins on beta cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
GM CSF with IFN gamma , but not with LPS , consistently activated the monocytes for TNF alpha activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of intercellular adhesion molecule 1 ( ICAM 1 ) on primary human fibroblasts , a human fibrosarcoma , chondrosarcoma , and adenocarcinoma cell line in response to IL 1 , TNF alpha , or IFN gamma was studied using an ELISA with anti ICAM 1 mAb . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Combination of recombinant tumor necrosis factor ( TNF ) and IFN gamma resulted in a synergistic enhancement of DR mRNA and protein induction in both cell lines . ^^^ THP 1 , but not U 937 , cells secreted TNF under the influence of IFN gamma . ^^^ Neutralization of TNF by a specific antibody decreased IFN gamma induced DR antigen expression in THP 1 cultures . ^^^ These observations indicate that TNF is not able to directly induce DR gene expression , but rather amplifies ongoing expression of this gene , whether constitutive or induced by IFN gamma . ^^^ In the two cell lines tested , the level of DR inducibility under the influence of IFN gamma used alone depended on a different inducibility of TNF secretion by IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effect of endogenous TNF on antilisterial resistance was due to neither regulation of alpha interferon ( IFN alpha ) and IFN gamma production nor induction of IFN beta subtype 1 ( IFN beta 1 ) , because anti TNF antibody treated mice produced normal levels of IFN alpha and IFN gamma in the bloodstream during infection and administration of monoclonal anti murine IFN beta 1 antibody had no effect on the development of listeriosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor selective cytotoxic effects of murine tumor necrosis factor ( TNF ) and interferon gamma ( IFN gamma ) in organ culture of B 16 melanoma cells and heart tissue . ^^^ Organotypically cultured , confronting pairs of B16B16 cell clusters and fragments of embryonic chick or mouse heart , as used for the study of invasion in vitro , were treated with murine TNF plus IFN gamma for 4 and 7 days . ^^^ Using this organ culture assay , which more closely mimics the situation in vivo , we confirmed the tumor selective cytotoxic effects of combined treatment with TNF and IFN gamma as observed in separate cultures of malignant and non malignant cells on `` artificial ' ' substrate . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study , we have used fluorescence photobleaching recovery ( FPR ) to quantify the effects of the cytokines tumor necrosis factor ( TNF ) and immune interferon ( IFN gamma ) on the lateral mobilities of class 1 major histocompatibility complex protein , of an abundant 96 , 000 Mr mesenchymal cell surface glycoprotein ( gp 96 ) , and of a phospholipid probe in cultured human endothelial cell ( HEC ) membranes . ^^^ Cultures treated at visual confluence for 3 4 d with either 100 U / ml TNF or 200 U / ml IFN gamma did not exhibit significant changes in protein or lipid mobilities despite significant changes in cell morphology and membrane antigen composition . ^^^ In HEC cultures treated concomitantly with TNF and IFN gamma , however , diffusion coefficients decreased by 71 79 % for class 1 protein , 29 55 % for gp 96 , and 23 38 % for Fl PE . ^^^ Finally , although TNF and IFN gamma retarded the growth of HEC cultures and disrupted the organization of cell monolayers , the slow diffusion rates of gp 96 and Fl PE in confluent doubly treated monolayers were not reproduced in sparse or subconfluent untreated monolayers . ^^^ We conclude that the slowing of protein and lipid diffusion induced by the combination of TNF and IFN gamma is not due to plasma membrane corrugations , to anisotropic diffusion barriers , or to decreased numbers of cell cell contacts . ( ABSTRACT TRUNCATED AT 400 WORDS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The modulation of growth of normal and leukemic myeloid progenitor cells in soft agar cultures by recombinant human tumor necrosis factor alpha ( TNF alpha ) and recombinant human interferon gamma ( IFN gamma ) was investigated . ^^^ However , when IFN gamma was administered together with TNF alpha , synergistically enhanced antiproliferative effects were detected in all colony types tested . ^^^ The antiproliferative action of IFN gamma on myelopoiesis was enhanced in culture by the presence of autologous monocytes , presumedly by inducing endogenous production of TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor Necrosis Factor alpha ( TNF alpha ) and Interferon gamma ( IFN gamma ) significantly enhanced the elastin degrading activity present in the culture media of both synoviocytes and chondrocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the in vivo effect of recombinant interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) treatment of mice on the development of the delayed type hypersensitivity ( DTH ) reaction and lichenoid tissue reaction ( LTR ) following the local injection of cloned autoreactive T cells . ^^^ Both the DTH reaction and the LTR were significantly enhanced by pretreatment with IFN gamma , but not with TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukins 1 , 2 , and 4 ( IL 1 , 2 , 4 ) , tumor necrosis factor alpha ( TNF alpha ) , alpha interferon ( IFN alpha ) , and gamma interferon ( IFN gamma ) , were incubated with tissue culture lines of murine tumor cells displaying low ( MCA 101 ) , intermediate ( MCA 102 , 106 ) , or high ( MCA 105 ) Class 1 expression . ^^^ Mice bearing pulmonary metastases or s . c . lesions generated by MCA 101 and 102 were treated with IFN alpha or IFN gamma i . p . or i . v . , or with a single dose of TNF alpha i . v . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokines gamma interferon ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and tumor necrosis factor beta ( TNF beta , lymphotoxin ) have potent in vitro antitumor activity and some clinical toxicities similar to interleukin 2 ( IL 2 ) / LAK . ^^^ IFN gamma was detected by enzyme linked immunosorbent assay , TNF alpha by WEHI 164 bioassay or enzyme linked immunosorbent assay , and TNF beta by WEHI 164 cell bioassay . ^^^ During the prime , few patients manifested in vivo detectable serum cytokines : IFN gamma , three of ten , 5 day prime ( 1 . 03 + / 0 . 46 ng / ml ) , and zero of five , 3 day prime ; TNF alpha , one of ten , 5 day prime , and one of three , 3 day prime ; TNF beta , one of ten , 5 day prime . ^^^ The supernatants of in vitro LAK generation cultures had detectable levels of cytokines at 24 h which increased progressively until culture harvest at Day 4 ( IFN gamma , 2 . 56 + / 0 . 34 ng / ml ; TNF alpha , 356 + / 110 pg / ml ; TNF beta , 8 . 2 + / 4 . 4 units / ml ) . ^^^ The highest levels of in vivo serum cytokines occurred following LAK cell infusion and were more often elevated in patients receiving rIL 2 by bolus than by continuous infusion : IFN gamma , four of six bolus , zero of three continuous infusion ; TNF alpha , six of six bolus ( maximum 679 pg / ml ) versus two of three continuous infusion ( maximum , 106 pg / ml ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Effects of tumor necrosis factor alpha ( TNF ) and interferon beta ( IFN beta ) on the IFN gamma induced major histocompatibility complex ( MHC ) class 2 expression on human umbilical vein endothelial ( HUVE ) cells are reported . ^^^ TNF inhibited the induction of MHC class 2 expression by IFN gamma markedly , when added before or simultaneously with IFN gamma . ^^^ However , TNF added to the cells 24 h after IFN gamma enhanced the expression of MHC class 2 antigens . ^^^ Addition of IFN beta , TNF , IFN gamma , and the combination of IFN beta and IFN gamma or TNF and IFN gamma , resulted in all cases in an enhanced MHC class 1 antigen expression . ^^^ The enhancing effect of TNF could not be inhibited by anti IFN beta indicating that TNF mediates enhancement of IFN gamma induced MHC class 2 expression via a pathway other than IFN beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this system we find that the basal level of adhesion can be increased in a dose dependent manner by pre treatment of the endothelial cells with interferon gamma ( IFN gamma ) and / or tumour necrosis factor ( TNF ) but not with interleukin 1 ( IL 1 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma alone at less than or equal to 10 , 000 U / ml is insufficient to elicit TNF alpha release . ^^^ However , when PBMC are first primed with IFN gamma for 48 h and then exposed to lymphokine supernatant for 6 h , effector cells within the PBMC population are triggered to express TNF alpha mediated cytotoxicity . ^^^ Although IFN gamma serves to prime PBMC in this assay system , it fails to trigger the release of TNF alpha . ^^^ Instead , a second lymphokine ( provisionally termed `` cytotoxicity triggering factor ' ' ( CTF ) is required to induce TNF alpha release from IFN gamma primed human PBMC . ^^^ On the basis of multiple criteria , CTF is distinguishable from several other cytokines : IFN gamma , IL 1 , IL 2 , GM CSF , MIF , CSF 1 , TNF alpha , and lymphotoxin ( TNF beta ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have previously reported that the cytokines IFN gamma and TNF alpha each upregulate the expression of class 1 MHC proteins and , in combination , induce the expression of class 2 MHC proteins on pancreatic islet cells . ^^^ IFN gamma and TNF alpha are therefore implicated in the immunologic destruction of beta cells in insulin dependent diabetes mellitus . ^^^ The objective of the present study was to define the effects of IFN gamma and TNF alpha on the function and viability of murine pancreatic islet beta cells in vitro . ^^^ Exposure of islets for 3 days to 200 U / ml of either IFN gamma or TNF alpha did not affect glucose stimulated insulin release , but at higher concentrations ( 2000 U / ml ) of either cytokine there was significant inhibition of glucose stimulated insulin release . ^^^ In combination , IFN gamma and TNF alpha each at 200 U / ml caused significant inhibition of glucose stimulated insulin release ; at 2000 U / ml glucose stimulated insulin release was abolished . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Elevated H2O2 release in response to PMA was observed in resident macrophages after a 48 h incubation with IFN gamma , TNF alpha , TNF beta , or CSF GM . ^^^ Although neither TNF alpha nor TNF beta induced NO 2 synthesis on its own , addition of either cytokine together with IFN gamma increased macrophage NO 2 production up to six fold over that in macrophages treated with IFN gamma alone . ^^^ These data suggest that : 1 ) tested as a sole agent , IFN gamma was the only one of the 12 cytokines capable of inducing both NO 2 and H2O2 release ; 2 ) the pathways leading to secretion of H2O2 and NO 2 are independent ; 3 ) either IFN gamma and TNF alpha / beta or IFN alpha / beta / gamma and LPS can interact synergistically to induce NO 2 release . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To test a hypothesis that immune mechanisms may be involved in the disease process in cattle persistently infected with and immunotolerant to bovine viral diarrhea virus , the effect of interferon alpha 1 1 ( IFN alpha 1 1 ) and gamma ( IFN gamma ) and tumor necrosis factor ( TNF alpha ) on bovine endothelial , epithelial , and fibroblast cell class 2 major histocompatibility complex ( MHC ) gene product expression was investigated . ^^^ However , following exposure to either recombinant bovine IFN gamma or TNF alpha ( rBoIFN gamma , rBoTNF alpha ) MHC class 2 ( Ia ) antigen expression was induced on these nonlymphoid cell types . rBoIFN alpha 1 1 did not induce class 2 antigens , but suppressed their induction by rBoIFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We then tested the ability of human recombinant alpha interferon ( IFN alpha ) and gamma interferon ( IFN gamma ) to interact with TNF to inhibit melanoma colony formation . ^^^ On a unit basis , IFN gamma was more active with TNF than IFN alpha . ^^^ The enhanced killing effect seen with the combination of IFN alpha , IFN gamma , and TNF suggests an interesting strategy for the treatment of human melanoma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using radio immunoassay methods , the production of tumour necrosis factor alpha ( TNF alpha ) , interleukin 2 ( IL 2 ) , and interferon gamma ( IFN gamma ) released by peripheral blood mononuclear cells ( PBMC ) , maintained in culture and stimulated by phytohemagglutinin ( PHA ) , was measured in normal subjects and patients with active or inactive rheumatoid arthritis ( RA ) . ^^^ The PBMC of patients with active RA produced less TNF alpha , IL 2 , and IFN gamma than did those of the controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The LDA is not neutralized by antibodies to G CSF , GM CSF , IFN alpha , IFN gamma , TNF , urokinase , and tissue plasminogen activator , and is not inhibited by preincubation with aprotinin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In two CMML patients investigated CFU C proliferation appeared to some extent inhibited by the addition of IFN alpha , IFN gamma and TNF alpha to cell cultures . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Synergistic enhancement of the antitumor activity of recombinant human TNF alpha by recombinant human IFN gamma . ^^^ The effect of recombinant human interferon gamma ( rHu IFN gamma ) on the antitumor activity of recombinant human tumor necrosis factor ( rHu TNF alpha ) was examined in vitro and in vivo . rHu IFN gamma enhanced both cytostatic and cytocidal activity of rHu TNF alpha against most rHu TNF alpha sensitive tumor cells in vitro . ^^^ However , there was no correlation between the degree of enhancement by rHu IFN gamma and that of the susceptibility of tumor cells to rHu TNF alpha . ^^^ The enhancing effect of rHu IFN gamma was most marked when tumor cells were treated with rHu IFN gamma either for 1 day before treatment with rHu TNF alpha or for the first day of the exposure to rHu TNF alpha . ^^^ A marked enhancing effect of rHu IFN gamma was also observed in the in vivo antitumor activity of rHu TNF alpha against HMV 2 melanoma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human malignant glioma cell lines and clones were incubated with various concentrations of recombinant human TNF alpha , either alone or in combination with recombinant human IFN gamma . ^^^ After incubation with both TNF alpha and IFN gamma , the surface expression of HLA ABC antigens was only slightly higher than that observed with each cytokine alone . ^^^ In contrast to IFN gamma , TNF alpha had no effect on the surface expression of HLA DR ( class 2 ) antigens . ^^^ Moreover , the surface expression of HLA DR induced by IFN gamma was unaffected by TNF alpha . ^^^ The increased expression of HLA ABC antigens after treatment with TNF alpha or IFN gamma correlated with increased levels of HLA ABC specific mRNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The capacity of IL 1 beta , TNF , and IFN gamma to stimulate platelet activating factor ( PAF ) synthesis by human monocytes is examined in our report . ^^^ Specific antibodies to TNF or IL 1 beta inhibited the late peak of PAF synthesis induced by IFN gamma indicating that late PAF synthesis is dependent on cytokine synthesis . ^^^ Biphasic platelet activating factor synthesis by human monocytes stimulated with IL 1 beta , tumor necrosis factor , or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To determine whether the production and secretion of TNF and IL 1 by human mononuclear phagocytes could be independently modulated , we examined secretion of TNF and IL 1 by fresh monocytes and monocytes pretreated with IFN gamma or granulocyte macrophage CSF before LPS stimulation . ^^^ The capacity to secrete TNF in response to LPS decreased slightly in cultured monocytes but was markedly augmented by IFN gamma ( approximately five fold more than fresh monocytes ) . ^^^ Additional evidence that TNF and IL 1 can be differentially modulated was the selective decrease in TNF secretion and the failure of IFN gamma to enhance TNF secretion by cultured monocytes from neonates , whereas results for IL 1 were similar with adult and neonatal monocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MDNCF mRNA was increased greater than 10 fold within 1 h after stimulation with LPS , IL 1 , or TNF , but not by IFN gamma , IFN alpha , or IL 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Other cytokines , IL 2 , IFN gamma , IFN alpha beta , granulocyte CSF , granulocyte macrophage CSF , IL 3 , TNF alpha , and TNF beta were compared to IL 1 for their ability to induce a PMN influx into the peritoneum . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor ( TNF ) was as effective as IL 1 in promoting tumor cell adhesion to EC , whereas IFN gamma and IL 2 were inactive . ^^^ Tumor necrosis factor ( TNF ) was as effective as IL 1 in promoting tumor cell adhesion to EC , whereas IFN gamma and IL 2 were inactive . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Its expression was regulated independently of the activation state of the cells , as revealed by treating M phi in vitro with bovine rIFN alpha I 1 , IFN gamma , or TNF alpha , all potent M luminal diameter activators . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After stimulation with rIL 2 , LAK cells produced significant levels of IFN gamma , TNF alpha , and TNF beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The recombinant cytokines IFN gamma and TNF alpha stimulate several macrophage mediated functions important in host defense . ^^^ Sequential aerosolization of IFN gamma and TNF alpha significantly increased both IL 1 release and Ia expression compared to either cytokine administered alone . ^^^ We conclude that aerosol administration of IFN gamma or TNF alpha enhances both pulmonary and systemic monocyte function , and that the combination of IFN gamma and TNF alpha produce additive or synergistic effects . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neither antibody reacted to recombinant mouse IL 1 alpha , IL 4 , TNF alpha , or IFN gamma in blot ELISA assays . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Because cytokines may have mediated some or all of the changes observed , we measured the capacity of splenocytes obtained at various times after infection to produce IL 2 , TNF , and IFN gamma in vitro . ^^^ TNF activity increased in parallel with the first and second LAA peaks , whereas increases in IL 2 and IFN gamma activity were associated only with the second . ( ABSTRACT TRUNCATED AT 400 WORDS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The identification and partial characterization of a human mononuclear cell derived neutrophil chemotactic factor apparently distinct from IL 1 , IL 2 , GM CSF , TNF and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF production was equivalent to that induced by lipopolysaccharide ( LPS ) and was enhanced by pretreatment of the macrophages with interferon gamma ( IFN gamma ) or with indomethacin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study we have investigated the effects of IL 1 and two other cytokines , namely tumor necrosis factor ( TNF ) and interferon gamma ( IFN gamma ) on the pancreatic B cell paying particular attention to insulin production and glucose metabolism . ^^^ The islets were subsequently transferred to media containing medium RPMI 1640 plus 0 . 5 % human serum with or without additions of human recombinant preparations of either IL 1 ( 25 U / ml ) , TNF ( 1000 U / ml ) , or IFN gamma ( 500 U / ml ) , and cultured for another 48 h . ^^^ TNF and IFN gamma were essentially without effect on islet morphology or function . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There was no interference of medium , serum , plasma , spinal fluid , or urine and no cross reaction with natural or recombinant IL 1 alpha , IL 1 beta , IL 2 , IFN gamma , or lymphotoxin ( TNF beta ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It is well documented that the antitumor capacity of tumor necrosis factor ( TNF ) can be enhanced by interferons ( IFNs ) , notably IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The factor ( s ) in the tumor conditioned medium appeared to be distinct from IL 1 , fibroblast growth factor , IFN gamma , TNF , endotoxin , TGF alpha , and TGF beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum adsorption studies demonstrated that IL 1 and TNF inducible endothelial target antigens are distinct from IFN gamma inducible antigens . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This lymphokine was distinct from IFN gamma , TNF and CSF 1 and may account for a principal mechanism of T APC signalling . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
At equivalent half maximal concentrations ( based on L 929 cytotoxicity units ) LT and TNF each caused rapid and transient induction ( peak 4 to 6 hr ) of an antigen associated with leukocyte adhesion ( detected by monoclonal antibody H4 / 18 ) , a rapid but sustained increased expression ( plateau 24 hr ) of a lymphocyte adhesion structure ( ICAM 1 ) , a gradual ( plateau 4 to 6 days ) increase in expression of HLA A , B antigens , and gradual ( 4 to 6 days ) conversion of HEC culture morphology from epithelioid to fibroblastoid , an effect enhanced by immune interferon ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A variety of factors may contribute to the regulation of Class 2 expression by thyrocytes : this is induced by interferon ( IFN gamma ) , and is enhanced by thyroid stimulating hormone ( TSH ) and by tumour necrosis factor ( TNF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Whereas monocytes do not accumulate CSF 1 messenger ( m ) RNA constitutively and consequently do not produce CSF 1 protein , CSF 1 mRNA and protein secretion became detectable , when monocytes were cultured in the presence of TNF alpha , that was synergistically enhanced by interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The results indicate that ( a ) the 26K responsive human lymphoblastoid CESS cells express about 1500 high affinity ( Kd = 30 pM ) binding sites for this cytokine , ( b ) this binding is not competed for by interleukin ( IL ) 1 , IL 2 , tumor necrosis factor ( TNF ) , IFN alpha 2 , IFN beta or IFN gamma , and ( c ) these 26K binding sites are different from the classical type 1 ( alpha beta ) IFN receptors by sodium dodecyl sulfate polyacrylamide gel electrophoresis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , a significant enhancement in PMN mediated antibody dependent cellular cytotoxicity was observed after treatment for 2 hr with IFN gamma and both TNF alpha and TNF beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The results demonstrate that the production of TNF alpha and TNF beta can be enhanced by two lymphokines , IL 2 and IFN gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Nitric oxide synthase ( NOS ) activity was enhanced in human umbilical vein endothelial cells ( HUVECs ) by the combined stimulation with IFN gamma plus IL 1beta , TNF alpha and LPS which was accompanied by cell death . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To test this hypothesis , we investigated the production of LT , TNF alpha , and interferon gamma ( IFN gamma ) by CD4+ T cell lines ( TCLs ) specific for myelin basic protein ( MBP ) or tetanus toxoid ( TT ) isolated from MS patients and normal controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although the precise mechanism of action is still unknown , cytokine antagonism is believed to be responsible for at least a part of its anti inflammatory / antipyretic influence : alpha MSH antagonizes pyrogenic and proinflammatory effects of cytokines such as interleukin 1 ( IL 1 ) , interleukin 6 ( IL 6 ) , tumor necrosis factor ( TNF ) , and interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast to the parental U 937 cell line , only tumor necrosis factor ( TNF ) alpha , and not interferon ( IFN ) gamma induces the expression of MHC class 2 antigens on C119 / 9 cells , and paradoxically , this induction was inhibited almost completely by IFN gamma . ^^^ Both HLA DR and DQ mRNA are induced by TNF alpha treatment , and both are diminished upon co treatment with TNF alpha and IFN gamma . ^^^ This antagonism between TNF alpha and IFN gamma seem to be restricted to MHC class 2 genes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Induced expression of mRNA for IL 5 , IL 6 , TNF alpha , MIP 2 and IFN gamma in immunologically activated rat peritoneal mast cells : inhibition by dexamethasone and cyclosporin A . ^^^ Stimulation of PMC with anti IgE for 4 hr induced the expression of mRNA encoding interleukin 5 ( IL 5 ) , IL 6 , tumour necrosis factor alpha ( TNF alpha ) , macrophage inflammatory protein 2 ( MIP 2 ) and interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
During the course of a secondary infection , serum concentrations of interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF ) are higher than during a primary infection . ^^^ Mice recovered from a sublethal primary Listeria infection , which acquired cell mediated immunity , received a subcutaneous injection of anti IFN gamma forming cells , or anti TNF forming cells , and 4 days later received an intravenous injection with 10 50 % lethal dose ( LD 50 ) L . monocytogenes . ^^^ The organs of immune mice treated with anti TNF forming cells yielded significantly more ( approximately 4 log 10 ) bacteria than those of immune control mice , more than those of immune mice treated with anti IFN gamma forming cells , and comparable numbers to those of non immune mice . ^^^ Taken together , these results demonstrate that TNF is essential in acquired resistance to L . monocytogenes during a secondary infection in mice , while IFN gamma plays a minor role . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We examined the effects of tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) on the production of collagen by human infant foreskin fibroblasts . ^^^ This value decreased from 10 . 3 % for IGF 1 cultured cells to 6 . 2 % and 8 . 4 % with the inclusion of TNF alpha and IFN gamma respectively , indicating that inhibition was selective for collagen . ^^^ TNF alpha ( 5 ng / ml ) and IFN gamma ( 250 U / ml ) together suppressed IGF 1 maintained collagen production to approximately 35 % , with a decrease from 10 . 3 % to 2 . 9 % in collagen production relative to total protein . ^^^ These data showed that IGF 1 maintained collagen formation is suppressed by the proinflammatory cytokines TNF alpha and IFN gamma , and that these interactions are influenced by dexamethasone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of cytokine message within induced sputum was examined by reverse transcription and the polymerase chain reaction ( PCR ) using primers specific for a range of cytokines ( IL 1 alpha , IL 2 , IL 3 , IL 4 , IL 5 , IL 6 , IL 8 , RANTES , TNF alpha , IFN alpha , IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Th 1 and Th 2 specific cytokine profiles ( interferon gamma ( IFN gamma ) and IL 4 ) and proinflammatory cytokines interleukin 1 ( IL 1 ) and tumour necrosis factor alpha ( TNF alpha ) in the conditioned media were analysed . ^^^ RESULTS Stimulation of SFMNC with bacterial antigen resulted in an increase in IFN gamma , IL 1 , and TNF alpha production which was accompanied by the induction of cartilage damage . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tuberculin induced expression of IFN gamma , IL 2 , IL 4 , IL 10 and TNF alpha mRNA occurred in the skin biopsies of all of the lesioned , M . bovis inoculated animals except for an absence of tuberculin induced TNF alpha mRNA expression in two animals . ^^^ By 28 weeks after vaccination , the three BCG vaccinated , sham inoculated cattle displayed minimal skin swelling response to tuberculin , but tuberculin induced expression of IFN gamma , IL 2 , IL 4 , IL 10 and TNF alpha mRNA was observed in skin biopsies of all of these animals . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A6H mAb binding induced a second signal in anti CD 3 mAb activated T cells , resulting in cell proliferation , IL 2 receptor expression and vigorous production of IFN gamma and TNF , and production of minor amounts of IL 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the expression of integrins , ICAM 1 , and CD 44 and the influence of interferon alpha ( IFN alpha ) , interferon gamma ( IFN gamma ) , and tumour necrosis factor alpha ( TNF alpha ) on expression of these molecules on four uveal melanoma cell lines . ^^^ IFN alpha , IFN gamma , and TNF alpha upregulated alpha 1 , alpha 2 , and alpha 3 expression , and did not alter alpha 4 , alpha 5 , alpha 6 , beta 2 , alpha 5 beta 3 , and beta 4 expression . ^^^ ICAM 1 was upregulated by IFN gamma and TNF alpha , but not by IFN alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro activation of macrophage NO synthase by IFN gamma induces a trypanostatic activity and TNF alpha is involved in NO synthase induction . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Its interaction with different cytokines [ interleukin 1 ( IL 1 ) , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) ] is important for lymphocyte migration into inflammatory sites . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The influence of alum and liposomal phospholipids on interferon gamma ( IFN gamma ) , IFN gamma / N acetylmuramyl L alanyl D isoglutamine ( MDP ) or IFN gamma / tumor necrosis factor alpha ( IFN gamma / TNF alpha ) induced macrophage nitric oxide ( NO ) synthesis has been investigated . ^^^ TNF alpha and MDP did not induce NO synthesis , but interacted synergistically with sub optimal doses of IFN gamma . ^^^ Alum , DPPC / PE and DPPC / PS liposomes also inhibited IFN gamma / MDP and IFN gamma / TNF alpha induced NO synthesis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study , normal ranges for the in vitro production by PBMCs of interleukin 1 beta ( IL 1 beta ) , tumor necrosis factor alpha ( TNF alpha ) , IL 2 , and gamma interferon ( IFN gamma ) were established ; the feasibility of using cryopreserved PBMCs for assays of in vitro cytokine production was evaluated ; and spontaneous ( unstimulated ) versus induced production of cytokines by fresh and cryopreserved PBMCs from healthy donors was compared . ^^^ Supernatants obtained from paired fresh and frozen PBMCs were quantitated for IL 1 beta , TNF alpha , IL 2 , and IFN gamma by using enzyme linked immunosorbent assay or a radioimmunoassay standardized against World Health Organization cytokine standards . ^^^ However , the mean levels of stimulated production of TNF alpha , IFN gamma , and IL 2 were significantly higher in cryopreserved versus fresh PBMCs ( P < or = 0 . 0004 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MLEC retained responsiveness to cytokines ( IL 1 alpha , IL 1 beta , TNF alpha , IFN gamma ) up to at least eight passages from primary culture and demonstrated upregulation of E selectin ( CD62E ) and P selectin ( CD62P ) mRNA as early as 2 hr after LPS stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The immunomodulator AS 101 has previously been found to induce mouse and human hematopoietic cells to secrete cytokines such as interleukin 1 alpha ( IL 1 alpha ) , IL 2 , tumor necrosis factor alpha ( TNF alpha ) , and gamma interferon ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In 78 patients ( 43 controls , 23 patients with PROM , 7 with preterm labour and delivery < 37 weeks of gestation , 7 with preterm labour and delivery at term ) amniotic fluid was collected and the following cytokines were measured by an ELISA : IL 1 beta , IL 2 , IL 6 , TNF alpha , IFN gamma , TNF receptor 55 und 75 , IFN gamma receptor , IL 2 receptor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the molecular basis of the synergistic induction by interferon gamma ( IFN gamma ) / tumor necrosis factor alpha ( TNF alpha ) of human interleukin 6 ( IL 6 ) gene in THP 1 monocytic cells , and compared it with the basis of this induction by lipopolysaccharide ( LPS ) . ^^^ Functional studies with IL 6 promoter demonstrated that three regions are the targets of the IFN gamma and / or TNF alpha action , whereas only one of these regions seemed to be implicated in LPS activation . ^^^ The three regions concerned are : 1 ) a region between 73 and 36 , which is the minimal element inducible by LPS or TNF alpha ; 2 ) an element located between 181 and 73 , which appeared to regulate the response to IFN gamma and TNF alpha negatively ; and 3 ) a distal element upstream of 224 , which was inducible by IFN gamma alone . ^^^ Synergistic induction of the IL 6 gene by IFN gamma and TNF alpha , in monocytic cells , involved cooperation between the IRF 1 and NF kappa B p 65 homodimers with concomitant removal of the negative effect of the retinoblastoma control element present in the IL 6 promoter . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neutralization of IFN gamma and TNF alpha caused a complete inhibition of nitrite production . ^^^ The present study showed that the ability of viable BCG to induce NO production is superior to that of killed BCG , and both IFN gamma and TNF alpha are essential for BCG induced NO production by spleen cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found that TNF alpha , like LPS , inhibited IFN gamma induced class 2 expression if NO was produced , but augmented it in the absence of NO formation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CD 18 cross linking primed neutrophils to activate the respiratory burst after stimulation with tumor necrosis factor alpha ( TNF alpha ) ( 100 units / mL ) , formylmethionyl leucyl phenylalanine ( fMLP ) ( 1 microM ) , and granulocyte macrophage colony stimulating factor ( GM CSF ) ( 1 micrograms / mL ) , but not granulocyte colony stimulating factor ( G CSF ) ( 1 micrograms / mL ) , interferon gamma ( IFN gamma ) ( 100 U / mL ) , or phorbol myristate acetate ( 100 nM ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When either anti tumor necrosis factor ( TNF ) , anti transforming growth factor beta ( TGF beta ) , or anti interferon gamma ( IFN gamma ) antibody was added to culture medium , suppressor activity was markedly reduced , in the order of anti TNF , anti IFN gamma , and anti TGF beta antibodies . ^^^ Therefore , TNF , IFN gamma , and TGF beta seem to be related to the full display of the suppressor function of MAC induced M phi s . ^^^ However , TNF alpha and IFN gamma but not TGF beta were substantially lacking in inhibitory action against SPC mitogenesis , when added exogenously . ^^^ On the other hand , both TNF alpha and IFN gamma potentiated the effector function of the suppressor M phi s . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) and Tumor Necrosis Factor alpha ( TNF alpha ) were detected in all samples tested . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
For macrophage activation we used lipopolysaccharide ( LPS ) and several recombinant cytokines ( IFN gamma , TNF alpha , IL 2 , IL 3 , IL 6 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The polymerase chain reaction ( PCR ) was used to detect the expression of IL 1 , IL 2 , IL 4 , IL 5 , IL 6 , TNF alpha , IFN gamma , TGF beta , TCR beta chain and aFGF genes in 21 myocardial biopsies obtained from 9 heart transplant patients . ^^^ IL 1 , IL 4 , IL 5 , CD 8 , IFN gamma , and TNF alpha were not detected in any of the biopsies . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To examine the potential contribution of endothelial cell cNOS ( ec cNOS ) and inducible NOS ( iNOS ) in controlling vascular tone under normal versus inflammatory conditions , we performed Northern hybridizations to examine the differential expression of each NOS mRNA in human aortic endothelial cells ( AOEC ) and human aortic smooth muscle cells ( AOSMC ) cultured for 8 h in the presence or absence of cytokines ( IL 1 beta , TNF alpha , and IFN gamma ) and LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here we show that interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) synergistically induced NO production and inducible NO synthase ( iNOS ) mRNA expression in mouse islet cells . ^^^ The combination of IFN gamma and TNF alpha , which is highly cytotoxic to mouse islet cells , failed to destruct islet cells in the absence of L arginine or in the presence of NG monomethyl L arginine ( NMMA ) . ^^^ These observations suggest that NO is a primary effector in islet cell damage caused by IFN gamma plus TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CD 44 and LFA 3 expression did not change after stimulation with IFN gamma or TNF alpha . ^^^ In contrast , ICAM 1 expression on human bronchial epithelial cells and BEAS 2B cells could be increased by incubation with PMA , IFN gamma , TNF alpha , and especially with the combination of IFN gamma and TNF alpha . ^^^ The maximal ICAM 1 expression on both epithelial cell types was obtained with the combination of TNF alpha and IFN gamma after 48 h of incubation . ^^^ The NCI H 292 cell line was different in that it only showed increased ICAM 1 expression after stimulation with PMA and IFN gamma and not by the combination of IFN gamma and TNF alpha or with TNF alpha alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Addition of rIL 1 beta as an accessory signal to anti CD 3 stimulated human T cells induced IL 6 mRNA expression and protein secretion , while IL 2 , IL 4 , GM CSF , IFN gamma , or TNF alpha did not have any effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , addition of either tumor necrosis factor alpha ( TNF alpha ) or interferon gamma ( IFN gamma ) , cytokines that by themselves had no effect on NOS activity , imparted LPS responsiveness onto these cells in a dose dependent manner ( EC 50 values of 39 ng / ml of TNF alpha and 9 . 4 U / ml of IFN gamma ) , and the effect of TNF alpha could be further potentiated ( twofold ) by the presence of interleukin 1 beta . ^^^ The simultaneous presence of TNF alpha and IFN gamma yielded a greater response than either cytokine alone ; however , the respective EC 50 values were not affected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
VCAM 1 expression by HEC was upregulated by cytokine pretreatment and the effects were ordered : IFN gamma > TNF alpha > IL 1 beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin 1 ( IL 1 ) , tumor necrosis factor ( TNF ) , interferon gamma ( IFN gamma ) and Escherichia coli lipopolysaccharide ( LPS ) increased cGMP at 24 h , whereas IL 2 and IL 6 were ineffective . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The organ cultures were supplemented with IFN gamma , tumour necrosis factor alpha ( TNF alpha ) , and granulocyte macrophage colony stimulating factor ( GM CSF ) , in a range of doses . ^^^ In cultured follicles , dose dependent induction of HLA class 1 , DR and ICAM 1 by IFN gamma , and HLA class 1 and ICAM 1 , but not HLA DR , by TNF alpha was observed in follicular epithelium , mainly in the outer root sheath ( ORS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The gamma / delta T cells were IL 2 dependent and expressed IFN gamma and TNF alpha , but not TNF beta , IL 2 , or IL 4 mRNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We describe a GAS associated , differentiation induced factor ( DIF ) as a potential molecular link between the activities of IFN gamma and of growth and differentiation factors . ^^^ DIF DNA binding was activated by colony stimulating factor 1 in murine macrophages and also during tetradecanoyl phorbol acetate induced differentiation or IFN gamma treatment in myeloid U 937 cells . ^^^ IFN gamma activation of DIF decreased significantly upon monocytic differentiation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Bacterial lipopolysaccharide ( LPS ) and interferon gamma ( IFN gamma ) were found to be effective inducers of 1 NOS mRNA , in accordance with their known ability to stimulate both 1 NOS activity and NO production in macrophages from different sources , while TNF alpha , IL 1 , or IL 6 was ineffective in this regard . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition to proliferation , CD40L induces lectin mediated cytolytic activity in thymic gamma delta T cells as well as the production of IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The ability of cytokines ( IFN alpha , IFN gamma , TNF alpha , IL 1 alpha , IL 6 ) , all trans retinoic acid , 1 , 25 ( OH ) 2 vitamin D 3 and the tumor promoting phorbol ester TPA to regulate cell surface expression of protectin ( CD 59 antigen ) on human hematopoietic and non hematopoietic neoplastic cell lines was examined with the aid of immunocytofluorometric measurements . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
For instance , CsA and FK 506 inhibit the transcription of IL 3 , IL 4 , IFN gamma , TNF alpha or GM CSF by activated T cells , and rapamycin has been shown to block the response to various growth factors such as IL 3 , IL 4 or IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , CD 44 9v and CD 44 6v were the main variants that were transiently upregulated on T cells after mitogenic stimulation and on myelomonocytic cell lines by TNF alpha and IFN gamma treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These results suggest that neutrophils may have a role in immunity against the parasites and that IFN gamma and TNF alpha are possibly involved . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study , we demonstrate that interleukin 4 ( IL 4 ) suppressed production of NO by primary mouse peritoneal macrophages exposed to IFN gamma with or without LPS , even while synergizing with IFN gamma to increase the secretion of TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of ICAM 1 was not significantly altered by tumour necrosis factor alpha ( TNF alpha ) ( P = 0 . 32 ) , IL 1 beta ( P = 0 . 24 ) , interferon gamma ( IFN gamma ) ( P = 0 . 66 ) or IL 4 ( P = 0 . 85 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Biological assays to measure interleukin 2 ( IL 2 ) or IL 4 , gamma interferon ( IFN gamma ) , and tumor necrosis factor and Northern ( RNA ) blot analysis to verify the expression of IL 2 , IL 4 , and IFN gamma revealed that the Th cell clones expressed a spectrum of cytokine profiles . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The stimulatory cytokines include interleukin 1 ( IL 1 ) , interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) and migration inhibitory factor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and interleukin 6 ( IL 6 ) , but not TNF beta , can induce the in vitro differentiation of the neuroblastoma cell line N 103 in a dose dependent manner . ^^^ The induction of neuroblastoma cell differentiation by TNF alpha and IFN gamma can be specifically inhibited by a nitric oxide ( NO ) synthase inhibitor , L NG monomethylarginine . ^^^ These results indicate that TNF alpha and IFN gamma , but not IL 6 , induce the differentiation of neuroblastoma cells via NO . ^^^ This is confirmed by the finding that the culture supernatants of N 103 cells induced by TNF alpha and IFN gamma , but not that by IL 6 , contained high levels of NO 2 , the production of which was inhibited by L NG monomethylarginine . ^^^ These data therefore indicate that NO may be an important mediator in the induction of neuronal cell differentiation by certain cytokines such as TNF alpha and IFN gamma and that neuronal cells , in addition to the macrophage like brain cells , can be induced by immunological stimuli to produce large quantities of NO . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The pattern of other cytokines induced by gp 120 includes interferon alpha ( IFN alpha ) and IFN gamma , tumour necrosis factor alpha ( TNF alpha ) , IL 6 , IL 1 alpha and beta , and not IL 2 and IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Infection with several DNA or RNA viruses induces a state of increased sensitivity to cell lysis mediated by tumor necrosis factor ( TNF ) , particularly in the presence of gamma interferon ( IFN gamma ) . ^^^ However , TNF and IFN gamma are known upregulators of HIV replication , raising the question of the potential role of these cytokines in the selective elimination of cells infected with this virus . ^^^ The present study demonstrates that chronically infected U 1 cells were killed with much greater efficiency by costimulation with TNF alpha and IFN gamma than their uninfected parental cell line U 937 . ^^^ Cell death in U 1 cells was not caused by the massive production of virions , in that costimulation with glucocorticoid hormones and TNF alpha or IFN gamma resulted in high levels of virion production without cytopathicity . ^^^ To investigate the nature of the selective cytotoxic effect observed in U 1 cells costimulated with TNF alpha plus IFN gamma , a panel of uninfected cell clones was generated by limiting dilution of U 937 cells and tested for response to TNF alpha and / or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This inhibitory activity of alveolar CD8+CD57+ cells is mediated by a glycosylated protein which is distinct from tumor necrosis factor alpha ( TNF alpha ) , TNF beta , transforming growth factor beta 1 ( TGF beta 1 ) , TGF beta 2 , interferon alpha ( IFN alpha ) , interferon gamma ( IFN gamma ) , and prostaglandins . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Production of TNF alpha , IL 4 , IL 5 , IL 2 , and IFN gamma was investigated in 15 CD4+CD56+ and in 18 CD4+CD56 T cell clones , which shared the same HLA restriction element ( DR2w15 ) and the same fine specificity ( peptide 193 207 of the S region ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 2 activated NK cells produced enhanced levels of IFN gamma and TNF in response to stimulation with anti CD 28 and PMA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma alone or in combination with TNF alpha strongly enhanced expression of HLA class 1 , HLA DR , DP , DQ , LFA 1 alpha and ICAM 1 , but did not affect expression of LFA 2 ( CD 2 ) and LFA 3 ( CD 58 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cell sorting experiments showed that the MBP specific donor cells were enriched for IFN gamma , IL 2 , TNF alpha , and IL 3 mRNA when compared with the host recruited spinal cord cells , whereas similar amounts of IL 10 mRNA were produced by both populations . ^^^ The spinal cord donor cells produced higher levels of IL 2 , IFN gamma , and IL 3 mRNA , whereas similar amounts of IL 10 and TNF alpha mRNA were produced from donor cells isolated from the spleen and the spinal cord . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunoassays detected IL 1 alpha ( 4 / 25 ) , IL 1 beta ( 12 / 25 ) , IL 6 ( 13 / 29 ) , IL 8 ( 29 / 29 ) , TGF beta 2 ( 5 / 12 ) and GM CSF ( 11 / 29 ) , but not IL 3 , IL 4 , TNF alpha , or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The following cytokines were used : interleukin 1 beta ( IL 1 beta ) , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , IL 6 , and IL 8 . ^^^ The levels of ICAM 1 expression were enhanced in a dose and time dependent manner by IL 1 beta , TNF alpha , or IFN gamma , but not by IL 6 or IL 8 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha alone did not directly induce macrophage NO 2 production to kill amebae ; however , in combination with increasing concentrations of TNF alpha and gamma interferon ( IFN gamma ) , BMM amebicidal activity and NO 2 production progressively increased and showed a significant linear correlation . ^^^ Antiserum to TNF alpha and the NO synthase inhibitor NG monomethyl L arginine ( L NMMA ) inhibited the synergistic effects of TNF alpha and IFN gamma . ^^^ BMM activated with increasing concentrations of lipopolysaccharide ( LPS ) and IFN gamma showed a significant linear correlation between TNF alpha release and NO 2 production . ^^^ Quantification by Northern ( RNA ) blot analyses demonstrated that IFN gamma in combination with TNF alpha or LPS increased markedly the accumulation of mac NOS and TNF alpha mRNAs in a time dependent manner with a concomitant increase in NO and TNF alpha production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When treated with the proinflammatory cytokines IFN gamma , TNF alpha , and IL 1 alpha , these fibroblast lines produce micromolar quantities of NO 2 and NO 3 , two stable end products of the NO pathway . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have examined cytokine regulation of nitric oxide synthase ( NOS ) in human umbilical vein endothelial cells ( HUVEC ) . 24 h treatment with IFN gamma ( 200 U / ml ) plus TNF ( 200 U / ml ) or IL 1 beta ( 5 U / ml ) increased NOS activity in HUVEC lysates , measured as conversion of [ 14C ] L arginine to [ 14C ] L citrulline . ^^^ IFN gamma plus TNF or IL 1 beta increased endogenous tetrahydrobiopterin levels and GTP cyclohydrolase 1 activity , the rate limiting enzyme of tetrahydrobiopterin synthesis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Islets from 30 human pancreata were exposed for 6 144 h to the following human recombinant cytokines , alone or in combination : IFN gamma ( 1 , 000 U / ml ) , TNF alpha ( 1 , 000 U / ml ) , IL 6 ( 25 U / ml ) , and IL 1 beta ( 50 U / ml ) . ^^^ Combinations of cytokines , notably IL 1 beta plus IFN gamma plus TNF alpha , induced increased expression of inducible NO synthase mRNA after 6 h and resulted in a fivefold increase in medium nitrite accumulation after 48 h . ^^^ An exposure of 144 h to IL 1 beta plus IFN gamma plus TNF alpha increased NO production and decreased both glucose induced insulin release and insulin content . ^^^ Inhibitors of NO generation , aminoguanidine or NG nitro L arginine , blocked this cytokine induced NO generation , but did not prevent the suppressive effect of IL 1 beta plus IFN gamma plus TNF alpha on insulin release and content . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In response to exposure to the inflammatory cytokines tumor necrosis factor alpha ( TNF ) and interferon gamma ( IFN gamma ) , murine brain microvascular endothelial cells ( MME ) synthesize the cell surface molecule , vascular cell adhesion molecule 1 ( VCAM 1 ) , and the intracellular enzyme , inducible nitric oxide synthase ( iNOS ) . ^^^ However , iNOS synthesis requires the presence of both TNF and IFN gamma , while VCAM 1 can be induced by either cytokine alone . ^^^ We examined the induction of VCAM 1 and iNOS under a variety of conditions to better define the regulation of TNF and IFN gamma signal transduction pathways in MME . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , dead rickettsiae ( which can not kill the IFN gamma treated RAW264 . 7 cells ) induced the production of as much TNF alpha as viable rickettsiae . ^^^ Inhibition of the production of TNF alpha ( by the addition of actinomycin D or emetine during the rickettsial infection ) or neutralization of TNF alpha ( by the addition of polyclonal rabbit anti mouse TNF alpha serum both during the IFN gamma treatment and during the rickettsial infection ) did not inhibit the killing of the RAW264 . 7 cells . ^^^ These data indicate that ( 1 ) TNF alpha , LPS , and the nitric oxide synthase pathway are not required in order for IFN gamma to prepare RAW264 . 7 cells to be killed by R . prowazekii ; ( 2 ) neither TNF alpha nor the nitric oxide synthase pathway is responsible for the killing of the IFN gamma treated RAW264 . 7 cells by R . prowazekii ; and ( 3 ) in cultures treated with IFN gamma plus LPS and then incubated with rickettsiae , a nitric oxide synthase pathway dependent mechanism inhibits the killing of the RAW264 . 7 cells . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The receptors studied were interleukin ( IL ) 1 receptor type 1 ( IL 1RI ) and type 2 ( IL 1RII ) , p 75 and p 55 tumor necrosis factor ( TNF ) receptors ( p75TNFR and p55TNFR ) , interferon ( IFN ) alpha / beta and gamma receptors ( IFN alpha / beta R and IFN gamma R ) , granulocyte macrophage ( GM ) colony stimulating factors receptor alpha subunit ( GM CSFR ) , G CSF receptor ( G CSFR ) , M CSF receptor ( c fms , M CSFR ) , stem cell factor receptor ( c kit , SCFR ) , IL 6 receptor ( IL 6R ) , and IL 8 receptor ( IL 8R ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The proliferative effect of anti CD 28 and IL 12 is resistant to moderate doses of cyclosporin A and is largely independent of endogenous IL 2 , IL 12 , in synergy with anti CD 28 or B 7 transfected cells , is most effective in inducing interferon gamma ( IFN gamma ) production , but production of tumor necrosis factor alpha and granulocyte / macrophage colony stimulating factor is also observed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Paradoxically , IFN gamma ( 50 micrograms ) , a cytokine that usually synergizes with TNF , inhibited LPS induction of splenic PTHrP messenger RNA ( mRNA ) levels in LPS sensitive C3H / OuJ ( OuJ ) and LPS resistant C3H / HeJ ( HeJ ) mice . ^^^ The stimulation of splenic PTHrP mRNA levels caused by the administration of TNF alpha or interleukin 1 beta was similarly inhibited by IFN gamma , a type 2 interferon . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Total RNA was prepared from brain , spinal cord , lung , spleen , liver and kidney of 6 to 8 week old specific pathogen free BALB / c mice by acid guanidinium thiocyanate phenol chloroform ( AGPC ) method . cDNA was synthesized by M MLV reverse transcriptase , and amplified using the specific oligonucleotide primers for IL 1 , IL 2 , IL 3 , IL 4 , IL 5 , IL 6 , TNF alpha , IFN alpha , IFN beta and IFN gamma by PCR method . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Total RNA was prepared from spleen lymphocytes 3 h after infection with HSV 1 ( +GC virulent variant and GCr attenuated variant of Miyama strain ) by acid guanidinium thiocyanate phenol chloroform ( AGPC ) method . cDNA was synthesized by M MLV reverse transcriptase , and amplified using the specific oligonucleotide primers for IL 1 , IL 2 , IL 3 , IL 4 , IL 5 , IL 6 , TNF alpha , IFN alpha , IFN beta and IFN gamma by PCR method . ^^^ After HSV 1 infection , IFN alpha , IFN beta , IFN gamma , IL 1 beta , IL 4 , IL 6 and TNF alpha mRNA were significantly induced , but IL 2 , IL 3 and IL 5 mRNA were not induced . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , the transgenic mice had significantly reduced production of IFN gamma in their livers and sera and TNF in their spleens . ^^^ These data indicate that AFP suppresses the production of the cytokines , IFN gamma and TNF in NK cells and macrophages so that the mice could not eliminate the bacteria . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our study confirmed the previously described features in lpr mice that IFN gamma , TNF alpha , and TNF beta were transcribed by various T cell subsets . ^^^ We also demonstrated that CD4+ T cells infiltrating into kidney tissues of MRL / lpr mice expressed various cytokine genes such as IL 1 , IL 5 , IL 6 , IFN gamma , TNF alpha , TNF beta and TGF beta . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , in the presence of enriched preparations of NK cells cultured together with the progenitor cells , NKSF / IL 12 inhibits formation of hematopoietic colonies supported by IL 3 and granulocyte macrophage CSF , by inducing production of IFN gamma and TNF alpha , two cytokines with synergistic inhibitory effects on hematopoietic colony formation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The demyelinative potential of the cytokines interleukin 1 alpha ( IL 1 alpha ) , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) has been investigated in myelinating aggregate brain cell cultures . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study , to determine which properties are shared by these strains , we examined R . prowazekii EVir for the following : ( 1 ) the sensitivity of its growth in L 929 cells to the cytokines IFN alpha / beta , IFN gamma , tumor necrosis factor alpha ( TNF alpha ) , and IFN gamma plus TNF alpha ; ( 2 ) the ability to grow in untreated RAW264 . 7 cells ; and ( 3 ) the ability to induce interferon in L 929 cell cultures ; we also evaluated strains 83 2P and 87 17 for lysine methylation . ^^^ In 10 irradiated L 929 cells , growth of strain EVir was slightly inhibited ( 11 % ) by TNF alpha alone , somewhat inhibited ( 38 % ) by IFN gamma alone , and markedly inhibited ( 87 % ) by IFN gamma plus TNF alpha . ^^^ Nitrite production was induced in 10 irradiated , strain EVir infected L 929 cell cultures treated with TNF alpha alone or IFN gamma alone ; however , more nitrite was produced in infected cultures treated with IFN gamma plus TNF alpha . ^^^ Nitrite production , the dramatic inhibitory effect of IFN gamma plus TNF alpha , and the modest inhibitory effect of IFN gamma on the growth of strain EVir in 10 irradiated L 929 cells were all alleviated by the addition of the nitric oxide synthase inhibitor NG methyl L arginine . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We measured soluble CD 14 ( sCD 14 ) , plasma endotoxin , tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 beta ( IL 1 beta ) , IL 6 , and interferon gamma ( IFN gamma ) in patients with multiple organ failure ( MOF ) . ^^^ In the septic group with MOF , the sCD 14 level correlated significantly with the TNF alpha level but not the plasma endotoxin , IL 1 beta , IL 6 , or IFN gamma level . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin 2 ( IL 2 ) activated killer cells , also referred to as lymphokine activated killer ( LAK ) cells , are stimulated by tumor cells to express cytotoxic activity and to also secrete cytokines such as interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This conversion in whole PMNs was not increased in vitro by LPS , IFN gamma , IL 1 beta , or TNF alpha nor decreased by W 13 , a calmodulin inhibitor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It has been well documented that cross linking CD 28 with mAb can provide a potent costimulatory signal to T cells for the production of IL 2 , IL 6 , IFN gamma , GM CSF and TNF alpha . ^^^ In contrast to the production of IL 2 , IFN gamma , GM CSF , and TNF alpha , the production of IL 4 after co stimulation with B 7 CD28 interaction was restricted to CD45RO+ memory T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have investigated iNOS expression and NO synthesis in rat vascular SMC treated with the cytokines , IFN gamma and TNF alpha , in the presence or absence of the activator of protein kinase C , beta phorbol 12 myristate 13 acetate ( PMA ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This activity is positively controlled by several up regulatory stimuli ( including IFN gamma , TNF alpha , IL 2 ) and negatively controlled by others ( principally IL 10 , IL 4 , TGF beta ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
As tumor necrosis factor alpha , IFN beta , and IL 12 are potent activators of IFN gamma production by T and NK cells , their presence in these cocultures could be responsible for the IFN gamma production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The inhibition of NO 2 and NO 3 release in mice injected with anti tumor necrosis factor ( TNF ) and / or anti interferon gamma ( IFN gamma ) monoclonal antibody ( mAb ) before SEB challenge revealed that both cytokines were involved in SEB induced NO overproduction . ^^^ In vitro experiments indicated that NO synthase ( NOS ) inhibition by N nitro L arginine methyl ester ( L NAME ) enhanced IFN gamma and TNF production by splenocytes in response to SEB . ^^^ A similar effect was observed in vivo as treatment of mice with L NAME resulted in increased IFN gamma and TNF serum levels 24 h after SEB challenge , together with persistent expression of corresponding cytokine mRNA in spleen . ^^^ Both TNF and INF gamma were responsible for the lethality induced by SEB in L NAME treated mice as shown by the protection provided by simultaneous administration of anti IFN gamma and anti TNF mAbs . ^^^ We conclude the SEB induces NO synthesis in vivo and that endogenous NO has protective effects in this model of T cell dependent shock by downregulating IFN gamma and TNF production . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Genistein also modulates the expression of ICAM 1 on endothelial cells by potentiating the upregulating effect of TNF and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Activation of CD4+ T cells with Chinese hamster ovary ( CHO ) DR4 / B7 and CHO DR4 / LFA 3 cells that present the superantigen staphylococcal enterotoxin A resulted in significant T cell proliferation and substantial production of TNF and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of Fas / APO 1 as well as Fas / APO 1 dependent cytotoxicity were augmented by preexposure of human malignant glioma cells to IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Because lymphokine production is critical for T cell functions , we have analyzed the capacity of melanoma specific tumor infiltrating lymphocyte ( TIL ) clones to produce major lymphokines : interleukin 2 ( IL 2 ) , interferon gamma ( IFN gamma ) and interleukin 4 ( IL 4 ) , as well as tumor necrosis factor ( TNF ) , in response to direct antigen presentation by autologous and allogeneic tumor cells . ^^^ We report here that , upon stimulation by autologous melanoma cells , all TIL clones secreted TNF but only a few of them produced significant amounts of IL 2 , IL 4 or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) , but not macrophage inflammatory protein 1 alpha ( MIP 1 alpha ) , cooperated with TGF beta 1 to reverse the proliferative effects of multiple stimulatory cytokines , resulting in 76 % inhibition . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , CD4XL resulted in the induction of interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) in the absence of interleukin 2 ( IL 2 ) and IL 4 secretion in PBMCs . ^^^ Both INF gamma and TNF alpha were found to contribute to Fas Ag upregulation and both anti IFN gamma and anti TNF alpha antibodies blocked CD4XL induced Fas Ag upregulation and lymphocyte apoptosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Upon stimulation with Tg , DH5D1 secreted little or no titers of IL 2 , TNF alpha , and IFN gamma , whereas activation with combination of phorbol myristate acetate and calcium ionophore produced measurable levels of these cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The alpha 1 chain , while absent in unstimulated synoviocytes , was induced by interleukin 1 beta ( IL 1 beta ) , tumour necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) . ^^^ These data suggest that IL 1 beta , TNF alpha , IFN gamma , and TGF beta are likely to be among the effectors regulating beta 1 integrin expression in synoviocytes in vivo . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CD4+ T cells as well as DNT cells constitutively expressed IFN gamma , TNF alpha , and TNF beta genes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma alone or in combination with TNF alpha enhanced nonspecific esterase activity but failed to induce CD 14 expression or increase eosinophilic granule containing cells . dbcAMP increased eosinophilic granule containing cells , nonspecific esterase activity and CD 14 expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NO release was increased by tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , interleukin 1 beta , lipopolysaccharide or forskolin in EC 219 cells , a rat brain microvessel derived EC line . ^^^ Tumor cells ( DHD / K12 / PROb , a rat colon carcinoma cell line ) were highly adherent to EC 219 cells , and adhesion was not modified by TNF alpha plus IFN gamma , or by dexamethasone . ^^^ NOS activity was increased by TNF alpha and IFN gamma , and significantly reduced by tumor cell conditioned medium . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These cells were examined by reverse transcriptase PCR for their expression of mRNAs for the following cytokines : granulocyte macrophage ( GM ) CSF , CSF 1 , TNF alpha , IFN gamma , TGF beta 1 , leukemia inhibitory factor ( LIF ) , and IL 2 . ^^^ Both decidual CD56brightCD16 NK cells and CD3+ T cells were found to express mRNA for CSF 1 , TNF alpha , IFN gamma TGF beta 1 , and LIF , but GM CSF mRNA was detected only in CD56bright NK cells . ^^^ In contrast , peripheral blood CD56brightCD16 NK cells , CD56dimCD16+ NK cells , and CD3+ T cells expressed mRNA only for TNF alpha and TGF beta 1 , but not for GM CSF , CSF 1 , IFN gamma , LIF , or IL 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha and IFN gamma stimulate a macrophage precursor cell line to kill Listeria monocytogenes in a nitric oxide independent manner . ^^^ In vivo studies have shown that both TNF alpha and IFN gamma play important roles in resistance against Listeria . ^^^ In this study we show that sequential stimulation with TNF alpha and IFN gamma induces the nonphagocytic , nonbactericidal mouse macrophage precursor hybrid cell line W1C3 to phagocytose and kill Listeria efficiently . ^^^ This provides the first direct evidence that TNF alpha and IFN gamma are both necessary and sufficient to induce macrophages to kill Listeria , and that they act directly on macrophages . ^^^ Data presented here also show that TNF alpha and IFN gamma induced the macrophages to produce large amounts of reactive nitrogen intermediates ( RNI ) , but complete inhibition of RNI generation did not decrease bactericidal activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neutralization of IFN gamma reduced immunity in vaccinated animals and resulted in decreased IFN gamma , IL 2 , IL 10 , TNF alpha , and IL 12 p 40 but markedly increased IL 4 , IL 5 , and IL 13 mRNA expression and serum IgE levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma and TNF alpha are two cytokines that are critical in the pathogenesis of EAE and MS . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Rather , the inhibition of cytokine production ( IL 2 , GM CSF , TNF , IFN gamma ) observed after 24 h of co culture may explain the inhibition of T cell DNA synthesis detected 3 days later . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Exposure of primary cultures of thyrocytes to interferon gamma ( IFN gamma ) , and even more , to the combination of IFN gamma plus tumour necrosis factor alpha ( TNF alpha ) induced a clear increase in the expression of NCAM as assessed by FACS analysis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When stimulated with HLA compatible target cells ( B LCL ) presensitized with cognate HIV 1 peptides , all clones produced GM CSF , TNF alpha , and IFN gamma and most produced low amounts of IL 2 , IL 3 , and IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The presence of tRA during in vitro MBP specific LNC activation resulted in a considerable increase in IL 4 mRNA , whereas mRNA for IL 2 , TNF alpha , and IFN gamma was decreased . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The addition of rmIL 12 during the in vitro stimulation with PLP resulted in a 10 fold increase in IFN gamma and a 2 fold increase in tumor necrosis factor ( TNF ) alpha in the supernatants , relative to LNC stimulated with PLP alone . ^^^ However , neutralization of IFN gamma or TNF alpha in vitro with specific antibodies did not abrogate the ability of rmIL 12 to exacerbate the subsequent disease . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
LPL T cells were significantly more responsive to CD 2 ligation than PBL , as determined by release of IFN gamma , IL 2 , IL 4 , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Monoclonal antibodies to tumour necrosis factor alpha ( TNF ) or interferon gamma ( IFN ) inhibited the myelosuppression partially ; simultaneous presence of both these antibodies completely abrogated this suppression , suggesting that both TNF alpha and IFN gamma were involved in amphotericin induced myelosuppression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon alpha ( IFN alpha ) and Interferon gamma ( IFN gamma ) , which had no effect alone , synergistically increased TNF alpha production when applied together with the enzymes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Binding of NK cells to plastic immobilized VN served as a costimulus with either anti Fc gamma RIII or IL 2 to produce IFN gamma , TNF alpha , and cell proliferation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the presence of a stimulating pair of anti CD 2 MoAb , T cell proliferation , production of cytokines ( IL 2 , IL 4 , IL 10 , GM CSF , IFN gamma and TNF alpha ) , and generation of cytotoxic T lymphocytes were all supported by B 7 1 ( + ) 3T6 cells but not by control 3T6 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , cytokines involved in macrophage activation , such as TNF and IFN gamma , were induced normally in NF IL 6 ( / ) mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RPE cell secretion of NO . was stimulated by the cytokines interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) , an effect enhanced by endotoxin [ lipopolysaccharide ( LPS ) ] , reduced by the competitive inhibitor of L arginine metabolism , NG monomethyl L arginine ( L NMMA ) and inhibited by cycloheximide . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 4 , IL 6 , tumour necrosis factor alpha ( TNF alpha ) , transforming growth factor beta ( TGF beta ) , interferon alpha ( IFN alpha ) and IFN gamma individually had no effect on GM CSF secretion , although IL 4 , TGF beta and IFN alpha all inhibited the action of IL 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Synergistic induction by IFN gamma plus TNF alpha and inhibition by IL 4 and IL 13 . ^^^ Little or no RANTES was produced after stimulation of HUVEC with IFN gamma , IL 1 beta , or TNF alpha . ^^^ Sequential treatment of HUVEC with TNF alpha and IFN gamma showed that IFN gamma sensitized HUVEC to the stimulating effect of TNF alpha . ^^^ These results indicate that the microenvironment of DTH granulomas , containing high levels of both TNF alpha and IFN gamma , may be responsible for RANTES production by perigranulomatous endothelial cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Astrocytoma cells up regulated surface VCAM 1 expression in response to cytokines in the following order : IFN gamma plus TNF alpha > IFN gamma plus IL 1 beta > TNF alpha > IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Study of IL 2 , IL 6 , TNF alpha , IFN gamma and beta in the serum and synovial fluid of patients with juvenile chronic arthritis . ^^^ In the present study , serum and synovial fluid levels of IL 2 , IL 6 , TNF alpha , IFN beta and IFN gamma were evaluated in a group of 66 patients with juvenile chronic arthritis ( JCA ) . ^^^ TNF alpha tended to be elevated but to a lesser extent , while synovial fluid IL 2 and IFN gamma were undetectable or very low , as in the serum . ^^^ TNF alpha levels were only slightly elevated ; on the other hand , IL 2 and IFN gamma were undetectable . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , determination of the expression of cytokine mRNAs in lesional skin of patients with active disease by reverse transcriptase polymerase chain reaction revealed transcripts for IL 1 beta , tumour necrosis factor alpha ( TNF alpha ) , IL 6 , IL 8 , IL 8R , IL 10 , interferon gamma ( IFN gamma ) , IL 2R and transforming growth factor beta ( TGF beta ) , but not IL 2 or IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We isolated 3 overlapping partial inducible NOS cDNA clones from a human glioblastoma cell line A 172 induced by IL 1 , TNF alpha , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We used an electrophoretic gel mobility shift assay to examine the activation of NF kappa B in human U 937 cells treated with tumor necrosis factor ( TNF ) , lymphotoxin ( LT ) , interferons ( IFN ) alpha , IFN beta , and IFN gamma , interleukins ( IL ) 1 beta , IL 4 , and IL 6 , leukemia inhibitory factor ( LIF ) , basic fibroblast growth factor ( FGF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and transforming growth factor beta ( TGF beta ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increased expression of both iNOS message and protein was seen in J 774 macrophages treated with IFN gamma and LPS and cardiac myocytes treated with TNF alpha , IL 1 beta , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha and IFN gamma , individually and in combination , upon primary cultures of human proximal tubular cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma , IL 1 beta , TNF alpha , and TGF beta 1 also enhanced phagocytosis , but IL 4 and IL 6 had no effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
BACKGROUND : We investigated the effects of tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) on HLA DR expression on human normal colonic epithelium , using an organ culture technique . ^^^ METHODS : Biopsy specimens from normal colonic mucosa were cultured for 24 h with various concentrations of TNF alpha and / or IFN gamma . ^^^ Concentrations of 10 ( 3 ) U / ml or higher of TNF alpha and 10 ( 2 ) U / ml or higher of IFN gamma induced expression of HLA DR antigens in a dose dependent manner . ^^^ Expression of HLA DR was increased in specimens cultured with both TNF alpha and IFN gamma compared with those cultured with either cytokine alone . ^^^ CONCLUSIONS : Our findings suggest that both TNF alpha and IFN gamma play important roles in regulating the expression of HLA DR on colonic epithelium in patients with inflammatory bowel disease . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have demonstrated : 1 ) that the B 7 1 expression of LC is reproducibly up regulated by either GM CSF , TNF alpha , IL 1 alpha , IL 1 beta , or IL 4 in a dose and time dependent manner , 2 ) that GM CSF exhibits the most active effect on B 7 1 up regulation in each experiment , 3 ) that IFN gamma or IL 10 profoundly inhibits the B 7 1 expression of LC in a dose and time dependent manner , and 4 ) that the down regulatory ability of IFN gamma or IL 10 neutralizes the activity of up regulatory cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Amoebic liver abscess derived macrophages [ days 10 , 20 , 30 post infection ( p . i . ) ] stimulated with interferon gamma ( IFN gamma ) and lipopolysaccharide ( LPS ) showed increased cytotoxicity against L 929 cells ( TNF alpha sensitive ) , but were refractory for killing amoebae and P 815 cells ( both NO sensitive ) , concomitant with low NO 2 production ( < 4 microM / 10 ( 6 ) cells ) . ^^^ Pretreatment of mouse bone marrow derived macrophages with amoebic proteins suppressed IFN gamma and LPS induced amoebicidal ( 33 % ) and tumoricidal ( 44 49 % ) activities , with a corresponding decrease in TNF alpha ( 56 % ) and NO ( 41 % ) production as well as TNF alpha ( 41 % ) and iNOS ( 27 % ) mRNA by Northern blot analyses as compared to untreated activated controls . ^^^ Inhibition of prostaglandin E 2 ( PGE 2 ) biosynthesis in abscess and naive macrophages pretreated with amoebic proteins augmented IFN gamma and LPS induced killing of L 929 cells and TNF alpha production , but failed to increase killing of P 815 cells and amoebae as well as iNOS mRNA levels or NO production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
First , both CD 40 and Ag receptor ( AgR ) cross linking induced purified tonsillar human B lymphocytes to secrete the same pattern of cytokines , including IL 1 beta , IL 6 , IL 10 , granulocyte macrophage CSF , and TNF alpha , whereas IL 1 alpha , IL 2 , IL 3 , IL 4 , IL 5 , IL 7 , granulocyte ( G ) CSF , or IFN gamma were not detected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To determine whether cytokine secretion patterns change with disease status in patients with multiple sclerosis ( MS ) , we measured IFN gamma , TNF alpha beta , IL 4 , IL 6 , IL 10 and TGF beta secretion in a panel of T cell clones ( TCCs ) specific for proteolipid protein ( PLP ) after stimulation with PLP peptides or polyclonal activators . ^^^ During acute attack , the predominant pattern of cytokine secretion resembled that of murine Th 1 cells ; i . e , IFN gamma and TNF alpha beta , and appeared to be restricted to PLP reactive TCCs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , interleukin 1 beta ( IL 1 beta ) , tumor necrosis factor alpha ( TNF alpha ) , or LPS in combination with interferon gamma ( IFN gamma ) stimulated iNOS mRNA , which was present within 4 h after exposure . iNOS mRNA levels were paralleled by changes in nitrite ( a metabolic product of NO ) . ^^^ Intraperitoneal administration of IFN gamma , TNF alpha , and LPS led to rapid induction of iNOS mRNA in lipocytes , confirming in vivo the culture findings . ^^^ Transforming growth factor beta 1 decreased IFN gamma / TNF alpha stimulated iNOS mRNA and nitrite . ^^^ In cells incubated with IFN gamma and TNF alpha , the contractile response to either serum or endothelin 1 was blocked . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The aims of this study were to investigate the role of cytokines ( tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) and interleukin 2 ( IL 2 ) in augmenting graft versus leukaemia ( GVL ) . ^^^ Supernatants from IL 2 treated and non treated bone marrow were tested for cytokine production ( TNF alpha and IFN gamma ) . ^^^ Measurable levels of TNF alpha but not IFN gamma were also detected in supernatants . ^^^ Addition of TNF alpha and IFN gamma to the IL 2 in the bone marrow cultures augmented the cytotoxicity but tended to inhibit progenitor cell growth as measured by granulocyte macrophage colony forming unit ( GM CFU ) and erythroid blast forming unit ( BFU e ) assays . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine signals , gamma interferon ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) act synergistically to induce production of a macrophage nitric oxide synthase ( NOS ) . ^^^ A variety of intracellular pathogens , when recognized by macrophages primed with IFN gamma , induce NOS by eliciting TNF alpha secretion , which then functions as a positive autocrine signal . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The data obtained suggest that iNOS is maximally induced in these cells by a 6 h exposure to IL 1 beta or TNF alpha + IFN gamma , but not by endotoxin . iNOS mRNA degradation is rapid and it is not affected by IL 1 beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Under these stimulation conditions , anergic T cells produced levels of IL 2 and TNF alpha that were comparable to their non anergized counterparts , whereas the levels of granulocyte macrophage CSF , IL 10 and IFN gamma production were even higher . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Indeed , fibronectin synthesis and secretion increased both in the EA . hy926 EC line and in human umbilical vein EC ( HUVEC ) after prolonged exposure to tumor necrosis factor alpha ( TNF alpha ) or interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of SMC with the combination of TNF alpha and interferon gamma ( IFN gamma ) synergistically enhanced nitrite release without having any additional effect on L arginine transport . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Bacterial products are without effect , but a combination of IL 1 and TNF alpha or IFN gamma is a potent stimulus . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , mRNA transcripts for IL 1 alpha , IL 1 beta , IL 6 , TNF alpha , MIP 2 , IFN gamma , and ICAM 1 from livers of treated mice were quantitated by reverse transcription polymerase chain reaction . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This effect was not abolished by the addition of monoclonal antibodies against tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , IL 1 alpha , IL 6 , G CSF , or granulocyte / macrophage CSF ( GM CSF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , after in vitro stimulation with interferon gamma ( IFN gamma ) , monocytes derived from GM CSF as well as from G CSF treated patients responded with a significantly higher TNF production and cytotoxicity than monocytes from control patients . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha , IL 2 , and IL 1 alone or together with IFN gamma neither induced iNOS nor primed bovine macrophages for enhanced iNOS expression or activity upon stimulation with S . dublin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of neuron cultures for 24 h with the combined stimulation of IFN gamma plus IL 1 beta , TNF alpha and LPS induced NOS activity by 87 fold which was calcium independent . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inflammatory cytokines such as tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) and interleukin 1 beta ( IL 1 beta ) play important roles in the mechanisms of hepatitis . ^^^ TNF alpha and IL 1 beta but not IFN gamma or IL 6 induced VCAM 1 expression on primary cultured murine hepatocytes in a dose and a time dependent fashion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In blood from G CSF treated subjects , our major findings were ( 1 ) a maximal 12 fold increase in interleukin 1 receptor antagonist ( IL 1ra ) release and an increase of both the p 55 and p 75 soluble tumor necrosis factor ( TNF ) receptors ; ( 2 ) a reduction in TNF release when using all the various stimuli described except LPS ; ( 3 ) an increase in G CSF and , to lesser extent , in IL 6 , IL 8 , and IL 10 release ; and ( 4 ) an attenuation of interferon gamma ( IFN gamma ) and granulocyte macrophage ( GM ) CSF release . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Femtogram concentrations of rTGF beta 1 suppressed MBP , PLP and PPD induced upregulation of IFN gamma , IL 4 , IL 6 , tumor necrosis factor alpha ( TNF alpha ) , TNF beta and perforin in MS , and acetylcholine receptor ( AChR ) induced augmentation of these pro inflammatory cytokines in MG , but had no effects on autoantigen or PPD induced expression of IL 10 or TGF beta itself . rTGF beta 1 also suppressed numbers of myelin antigen reactive IFN gamma and IL 4 secreting cells in MS and AChR reactive IFN gamma and IL 4 secreting cells in MG . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This tumor tissue specific reduction in blood flow was significantly inhibited by pretreatment with antisera against tumor necrosis factor ( TNF ) alpha , interferon ( IFN ) alpha / beta , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
L . major induced the production of two competing cytokines in infected macrophages : ( 1 ) the parasite activated the gene for tumor necrosis factor ( TNF ) , and production of TNF protein was enhanced by the presence of interferon gamma ( IFN gamma ) . ^^^ TNF then acted as a autocrine signal to amplify IFN gamma induced production of NO ; and ( 2 ) the parasite upregulated production of transforming growth factor beta ( TGF beta ) , which blocked IFN gamma induced production of NO . ^^^ Whether parasite induced TNF ( parasite destruction ) or TGF beta ( parasite survival ) prevailed depended upon the presence and quantity of IFN gamma at the time of infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha and IP 10 mRNA expression induced by IFN gamma and IL 2 was markedly more sensitive to suppression by oxidized LDL than that induced by LPS . ^^^ The cooperative effects of IFN gamma and LPS on the expression of the inducible nitric oxide synthase gene were suppressed by oxidized LDL while the antagonistic effect of IFN gamma on LPS induced expression of the TNF receptor type 2 mRNA was not altered . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In order to examine possible mechanisms leading to intestinal dysfunction following blood loss , we investigated mRNA levels for cytokines with proinflammatory and immunoregulatory properties ( interleukin 1 beta ( IL 1 beta ) , IL 6 , IL 10 , TNF alpha , TGF beta , IFN gamma ) as well as mRNA expression for inducible nitric oxide synthase ( NOS ) over the 3 days following hemorrhage and resuscitation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To investigate the molecular mechanisms underlying this phenomenon , we studied the effects of the inflammatory cytokines interleukin ( IL ) 1 , IL 6 , tumor necrosis factor alpha ( TNF ) , interferon ( IFN ) alpha , and IFN gamma on the expression of P 450 2C11 and the mRNAs of two typical acute phase protein genes , alpha 1 acid glycoprotein ( AGP ) and fibrinogen , in primary hepatocyte cultures . ^^^ IL 1 , IL 6 , TNF , and IFN alpha all suppressed P 450 2C11 mRNA , whereas IFN gamma had no effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Since TNF alpha can increase interferon gamma ( IFN gamma ) dependent nitric oxide ( NO ) production , we studied whether LPL may synergize with IFN gamma for the induction of macrophage NO production . ^^^ Finally , binding of nuclear proteins to the nuclear factor kappa B and TNF alpha responsive sequences of the macrophage NOS promotor was decreased by treatment of the cells by IFN gamma alone or in combination with LPL . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This cytokine synergy pattern differs from that reported for NK cells , in which IL 12 in combination with either IL 2 or TNF alpha induces NK cells to produce IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Th 1 derived cytokines ( IFN gamma , IL 2 , TNF alpha ) favor macrophage activation , whereas the Th 2 cytokines ( IL 4 , IL 10 , IL 13 ) exhibit suppressive activities on macrophage functions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Fas antigen expression on lymphocytes is regulated by interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) , cytokines that also have inhibitory effects on hematopoiesis . ^^^ Stimulation by TNF alpha and IFN gamma markedly increased Fas antigen expression on CD34+ cells . ^^^ Anti Fas antibody , which mimics the action of the putative ligand , enhanced IFN gamma and TNF alpha mediated suppression of colony formation by bone marrow ( BM ) in a dose dependent manner . ^^^ Colony formation from mature ( CD34+ CD38+ ) and immature ( CD34+ CD 38 ) progenitor cells and long term culture initiating cells were susceptible to the inhibitory action of anti Fas antibody in the presence of IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the current studies rat alveolar macrophages stimulated in vitro with murine interferon gamma ( IFN gamma ) , tumor necrosis factor alpha , interleukin 1 alpha , ( IL 1 alpha ) , lipopolysaccharide ( LPS ) , or IgG ICx immunostained for iNOS and produced nitrite / nitrate ( NO 2 / NO3 ) in a dose and time dependent manner requiring availability of L arginine . ^^^ Finally , in the immune complex model of alveolitis , the appearance of iNOS in macrophages as well as macrophage production in vitro of NO 2 / NO3 was dependent on the in vivo availability of tumor necrosis factor alpha , IL 1 , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
On stimulation with tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 beta ( IL 1 beta ) , interferon gamma ( IFN gamma ) , and lipopolysaccharide ( LPS ) , it was found that hepatocytes from all three species produce high levels of NO with levels of production exhibiting the following hierarchy : rat hepatocytes > mouse hepatocytes > human hepatocytes . ^^^ Whereas rat and mouse hepatocytes express inducible NO synthase messenger RNA ( mRNA ) in response to TNF alpha , IL 1 beta , or IFN gamma as a single stimulus , human hepatocytes respond to LPS alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Preexposure of the glioma cell lines to the cytokines , IFN gamma and TNF alpha , which sensitize for Fas / APO 1 dependent killing , partially overcame bcl 2 mediated rescue from apoptosis , suggesting that multimodality immunotherapy involving cytokines and Fas / APO 1 targeting might eventually provide a promising approach to the treatment of human malignant gliomas . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Collectively , these results suggest that gamma delta T cells play a role in priming macrophages to a steady state of activation via IFN gamma secretion , which allows them to produce TNF alpha when exposed to LPS . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Simultaneous incubation of primary rat bone marrow derived mononuclear phagocytes ( BMMo ) and tumor cells with gram negative agents triggers within 24 h interferon gamma ( IFN gamma ) and tumor necrosis factor ( TNF alpha ) independent tumoricidal activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Total RNA was prepared from the days 7 embryos by acid guanidinium thiocyanate phenol chloroform ( AGPC ) method . cDNA was synthesized by M MLV reverse transcriptase , and amplified using the specific oligonucleotide primers for IL 1 , IL 2 , IL 3 , IL 4 , IL 5 , IL 6 , TNF alpha , IFN alpha , IFN beta , IFN gamma , IFN alpha / beta receptor ( IFN alpha / beta R ) , IFN gamma receptor ( IFN gamma R ) , interferon reguratory factor ( IRF ) 1 , IRF 2 and 2 ' 5 ' oligoadenylate synthetase ( 2 5AS ) by PCR method . ^^^ Although the expressions of IL 1 beta , IL 4 , IL 5 , IL 6 , TNF alpha and IFN gamma mRNA were detected in all the embryos tested , the expressions of IL 2 , IL 3 , IFN alpha and IFN beta mRNA were not detected at all . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The addition of anti cytokine ( TNF alpha , IL 1 beta , or IFN gamma ) antibodies had no effect on the increase of these adhesion molecules . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We then tested the ability of several cytokines ( TGF beta , TNF alpha and IFN gamma ) to induce Fas mediated keratinocyte apoptosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cells were treated with interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , a combination of these cytokines , interleukins 3 and 6 ( IL 3 and IL 6 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and a combination of IL 3 and IL 6 for 48 h . ^^^ Fn was minimally induced in response to IFN gamma and TNF alpha , when compared with the untreated cells , whereas TNF alpha and especially the IFN gamma plus TNF alpha combination resulted in a prominent Tn induction . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Incubation of cells with TNF alpha or IFN gamma induced the expression of RANTES mRNA and protein within 16 h . ^^^ The combination of TNF alpha and IFN gamma displayed a marked synergism in inducing RANTES expression . ^^^ Pretreatment of cells with the glucocorticoid budesonide ( 10 ( 10 ) 10 ( 7 ) M ) for 24 h inhibited expression of RANTES mRNA and protein stimulated by either TNF alpha or TNF alpha plus IFN gamma in a concentration and time dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of DEA / NO were gene specific because DEA / NO did not affect the IFN gamma plus LPS induced expression of TNF alpha mRNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the expression of an apoptosis associated antigen ( Fas ) ( CD 95 ) on hematopoietic progenitor cells in the presence or absence of interferon gamma ( IFN gamma ) and / or tumor necrosis factor alpha ( TNF alpha ) . ^^^ However , IFN gamma and / or TNF alpha induced the expression of both the mRNA of Fas and Fas itself in a dose dependent fashion on the surface of CD34+ cells after 48 hours of serum free culture . ^^^ IFN gamma and TNF alpha had a synergistic effect on the induction of Fas , when both cytokines were added to the culture . ^^^ These observations indicate that IFN gamma and / or TNF alpha , well known as negative hematopoietic regulators , induce functional Fas on hematopoietic progenitor cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of all these antigens was upregulated by IFN gamma and TNF alpha . ^^^ The effect of these mAbs on TMF / thymocyte binding was studied using a simple rosette assay by incubating unstimulated or IFN gamma or TNF alpha stimulated TMF , grown on microscopic slides with resting or ConA+IL 2 activated thymocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of ICAM 1 by cell lines HT 29 and int 407 was increased by proinflammatory cytokines interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) and IL 1 but not by IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Hepatocytes costimulated with a combination of IFN gamma , interleukin 6 ( IL 6 ) , and tumor necrosis factor alpha ( TNF alpha ) expressed the highest antilisterial activities . ^^^ IFN gamma stimulated , L . monocytogenes infected hepatocytes expressed TNF alpha mRNA , suggesting that they are a source of this cytokine during listeriosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Characterization of infection included recording of signs and symptoms , excretion of S . sonnei in stool , measurement of humoral tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 beta ( IL 1 beta ) , interferon gamma ( IFN gamma ) , C reactive protein , IL 2 receptor , soluble CD 8 , antibody antigen complexes , and endotoxin . ^^^ Excretion of S . sonnei ( mean of time of the first positive cultures ) was followed in sequence by the onset of increases in TNF alpha ( 10 hr ) , liquid stools ( 14 hr ) , fever and dysentery ( 18 hr ) , IFN gamma ( 22 hr ) , and C reactive protein ( 34 hr ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma and TNF alpha , potent inhibitors of hematopoiesis , induce nitric oxide synthase ( NOS ) in various cell types . ^^^ Using PCR and immunoprecipitation , we found inducible NOS ( iNOS ) mRNA and iNOS protein in BM after stimulation with IFN gamma or TNF alpha . iNOS mRNA was also detected by PCR in highly purified CD34+ cells ; TNF alpha or IFN gamma increased iNOS expression . ^^^ NG Monomethyl L arginine ( MM Arg ) , an NOS inhibitor , partially reversed the effects of TNF alpha and , to a lesser extent , IFN gamma in methylcellulose culture of total BM and CD34+ cells , and inhibited apoptosis of BM cells induced by these cytokines . ^^^ When the effects of competitive iNOS inhibition were tested on more immature progenitors , MM Arg increased the number of long term BM culture initiating cells in control cultures but failed to protect these cells from the inhibitory action of IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
N4C cells produced IL 2 , IFN gamma , and TNF alpha beta , but not IL 4 and IL 5 after stimulation with TEC , thus exhibiting the profile of lymphokine production similar to that expressed by CD4+ Th 1 on one hand , but on the other , they showed the functional property that has not been observed for anti TEC CD4+ clones . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When recombinant cytokines were used , IL 1 beta , TNF alpha , and IFN gamma each induced iNOS activity in cardiac myocytes at 24 hours . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Effect of PGE 2 and of agents that raise cAMP levels on macrophage activation induced by IFN gamma and TNF alpha . ^^^ The effect of prostaglandin ( PG ) E 2 on macrophage activation by interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) was evaluated . ^^^ Murine macrophages infected with Leishmania enriettii or Leishmania major were activated by exposure to IFN gamma ( 10 50 U / ml ) and TNF alpha ( 30 3000 U / ml ) , leading to intracellular parasite destruction within 24 48 h . ^^^ Finally , PGE 2 and cAMP enhanced expression of inducible nitric oxide synthase mRNA in response to IFN gamma and TNF alpha , and this effect was inhibited by the cAMP antagonist 2 ' O methyl adenosine . ^^^ These findings are consistent with the hypothesis that PGE 2 acts as a positive agonist in macrophage activation by IFN gamma and TNF alpha via its capacity to modulate intracellular cAMP levels . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we report the expression of VCAM 1 in primary cultures of human brain microvessel endothelial cells ( HBMEC ) under standard conditions and following bacterial lipopolysaccharide ( LPS ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 beta ( IL 1 beta ) , or interferon gamma ( IFN gamma ) treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor derived factor synergizes with IFN gamma and LPS , IL 2 or TNF alpha to promote macrophage synthesis of TNF alpha and TNF receptors for autocrine induction of nitric oxide synthase and enhanced nitric oxide mediated tumor cytotoxicity . ^^^ TDRF synergized with IFN gamma and IL 2 to promote TNF alpha and mRNA synthesis and release by murine macrophages for increased autocrine induction of nitric oxide ( NO ) mediated tumor cytotoxicity . ^^^ TDRF containing culture fluid from L 1210 targets was subsequently confirmed to synergize with IFN gamma and rTNF alpha , LPS or IL 2 triggering agents to promote increased TNF alpha mRNA for autocrine induction of NOS mRNA synthesis with resultant augmentation of NO generation . ^^^ IFN gamma selectively upregulated TNF R 1 mRNA expression , whereas either IL 2 or LPS upregulated only TNF R 2 mRNA expression . ^^^ TDRF combined with IFN gamma to further upregulate TNF R 1 mRNA and with either IL 2 or LPS to further upregulate TNF R 2 , mRNA expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Encephalitogenic CDa+ T cell lines and clones derived from MBP sensitized DA rats secreted IFN gamma and TNF alpha and proliferated to MBP and MBP 89 169 , but not to MBP 68 88 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha and IFN gamma induce expression of nitric oxide synthase in cultured rat medullary interstitial cells . ^^^ To determine whether these cells can also produce NO , we studied the effects of tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) on NO production , NOS 2 mRNA , and NOS 2 protein expression . ^^^ Both TNF alpha and IFN gamma , in the presence of a low concentration of the other cytokine , caused dose dependent increases in NO production . ^^^ Exposure to TNF alpha and IFN gamma stimulated the production of NOS 2 mRNA , as determined by Northern blotting . ^^^ We conclude that 1 ) TNF alpha and IFN gamma induce the expression of vascular smooth muscle NOS 2 and production of NO in RMIC , and 2 ) NO acts as an autocrine activator of the soluble guanylyl cyclase in RMIC . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our results showed that U 937 cells secreted TNF alpha and IL 1 beta in response to either phorbyl 12 myristate 13 acetate ( PMA ) or IFN gamma + LPS . ^^^ AFP also inhibited interferon gamma plus lipopolysaccharide ( IFN gamma + LPS ) induced TNF alpha and IL 1 beta production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cyclosporin A ( CsA ) inhibited interleukin 2 ( IL 2 ) , IL 3 , interferon gamma ( IFN gamma ) , GM CSF and tumor necrosis factor alpha ( TNF alpha ) mRNA expression in spleen cells stimulated with concavalin A ( Con A ) when determined by the semi quantitative reverse transcription polymerase chain reaction ( RT PCR ) method . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
By PCR , no PR 3 expression was found in human umbilical vein endothelial cells ( HUVEC ) either untreated , or when treated with interferon gamma ( IFN gamma ) ( 200 U / ml , 6 h , 24 h ) , IL 1 ( 20 U / ml , 6 h ) , tumour necrosis factor alpha , ( TNF alpha ) ( 200 U / ml , 0 , 1 , 2 , 4 , 6 h ) or IFN gamma + TNF alpha ( 6 h ) ; iliac vein and artery endothelial cells did not express PR 3 either . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma stimulation induced surface human leukocyte antigen ( HLA ) DR in both SVRPE and HRPE , while TNF treatment induced surface expression of intercellular adhesion molecule ( ICAM ) 1 on SVRPE and upregulated ICAM from basal levels on HRPE . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Purified ALDC survived and grew for up to 30 days in lymph node conditioned medium and survived 14 days in recombinant ovine ( rov ) TNF alpha whereas none were detected after 24 h in rov GM CSF , rov IFN gamma or rh M CSF . ^^^ In contrast , when 50 200 units ml 1 of rov IFN gamma were added to cultures of ALDC along with TNF alpha or rov TNF alpha plus rov GM CSF , cell survival and growth was inhibited . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Addition of IL 1 , IL 3 , IL 5 , IL 6 , IL 9 , TNF alpha or IFN gamma was without effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The authors review their experience of 4 years with isolated limb perfusion for the application of high dose TNF alpha associated to IFN gamma and melphalan for the treatment of regionally advanced tumours such as malignant melanoma , soft tissue sarcoma and epidermoid carcinoma . ^^^ With the regional application of high doses of TNF alpha associated to chemotherapy and IFN gamma , it has been possible to validate the concept of a strategy based on a dual targeting , that is the selective impact of the intratumoral vessels by TNF alpha and of the tumour cells by chemotherapy . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Induction of enhanced cytokine secretion has been found to involve IFN alpha / beta , IFN gamma , TNF alpha , TNF beta , IL 1 , IL 6 , and the 40 kDa chain of IL 12 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , cotreatment of macrophages with both liposome MTP PE and IFN gamma resulted in the secretion of both tumor necrosis factor alpha ( TNF alpha ) and interleukin 6 ( IL 6 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We tested biopsy specimens of eight subcutaneous metastases that had developed inflammation following vaccine treatment for expression of mRNA for interferon gamma ( IFN gamma ) , interleukin 4 ( IL 4 ) , tumor necrosis factor alpha ( TNF alpha ) , and IL 10 . ^^^ Post vaccine , inflamed biopsies contained mRNA for IFN gamma ( 5 / 8 ) , IL 4 ( 4 / 8 ) or both ( 3 / 8 ) , and for TNF alpha ( 4 / 7 ) . ^^^ In contrast , IFN gamma mRNA was detected in only 1 / 17 and TNF alpha mRNA in 2 / 16 control specimens ( pre treatment lymph node metastases or non inflamed subcutaneous metastases ) . mRNA for IL 10 , a cytokine with anti inflammatory properties , was detected in 24 / 25 melanoma metastases and was independent of lymphoid content ; in situ the reverse transcriptase / polymerase chain reaction confirmed that melanoma cells were the major source . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The priming effect of TGF beta 1 on NO production was critically dependent on both a TNF alpha ( > or = 100 U ) and a LPS ( > or = 100 ng ) triggering dose in the presence of IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the brain of infected animals , interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) , interleukin 2 ( IL 2 ) , IL 6 , IL 10 and IL 12 mRNA were induced to a significant extent , but only low levels of IL 4 mRNA were detectable . ^^^ However , in contrast to the brain , the increase of IL 2 mRNA was particularly pronounced in the spleen , whereas the opposite was found for IFN gamma and TNF alpha mRNA . ^^^ Thus , cytokines involved in T cell proliferation were more prevalent in the spleen , but in the brain , where Tg actively multiplies , the effector molecules IFN gamma and TNF alpha were preferentially up regulated . ^^^ In addition , a detailed analysis of cytokine mRNA levels in major histocompatibility complex ( MHC ) congenic strains of BALB and B 10 mice revealed that the genetically regulated susceptibility to Tg was correlated with the amount of intracerebrally produced cytokine mRNA for IFN gamma , TNF alpha and IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The present work definitively demonstrates that a Ld restricted response decreases the number of organisms and cysts within the brain and thereby limits toxoplasmic encephalitis and levels of interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) , interleukin 2 ( IL 2 ) , IL 6 , IL 10 , transforming growth factor beta ( TGF beta ) , IL 1 alpha , IL 1 beta and macrophage inhibiting protein ( MIP ) mRNA in the brain 30 days after per oral infection . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of genes for tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 alpha ( IL 1 alpha ) , IL 1 beta , IL 6 , and gamma interferon ( IFN gamma ) in whole lung tissue and TNF alpha gene expression in bronchoalveolar lavage ( BAL ) cells was determined by reverse transcription PCR using specific cytokine primers at various times postinoculation . ^^^ In addition , concentrations of TNF alpha , IL 1 , IL 6 , and IFN gamma were determined in BAL fluid and serum samples at various times postinoculation . ^^^ Our results showed that there was a sequential appearance of cytokines in the lungs of infected mice : TNF alpha , produced primarily by BAL cells , appeared first , followed by IL 1 and IL 6 , which were followed by IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , the regulation of the expression of these molecules by the inflammatory cytokines interleukin 1 beta ( IL 1 beta ) , IL 6 , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) was determined . ^^^ Flow cytometric analysis determined that ICAM 1 was constitutively expressed on RPE cell lines and that in the presence of TNF alpha , IFN gamma , and IL 1 beta , there was a median fold increase in expression of 4 . 4 , 5 . 4 , and 4 . 4 , respectively . ^^^ After activation with IFN gamma , TNF alpha , and IL 1 beta , RT PCR analysis showed that the number of RPE cell lines expressing all the adhesion molecules increased . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To determine more specifically whether defined cytokines are able to enhance factor B and C 4 complement production , MCs were stimulated with IL 1 alpha , IFN gamma , and TNF alpha . ^^^ Factor B synthesis was increased in a dose dependent fashion by IL 1 alpha , TNF alpha , and IFN gamma , whereas C 4 synthesis was only upregulated by IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The TNF induced death of blasts is potently inhibited by IL 2 , as well as by IL 1 , IL 4 , IFN gamma , and IL 12 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Blood levels of TNF , IL 2 , IL 6 , and IL 10 were slightly more elevated in IFN gamma R alpha o / o mice , but insufficiently so to explain increased disease severity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The data that were derived from such studies show that the nonhuman primate cytokines IL 1 alpha , IL 1 beta , IL 2 , IL 4 , IL 5 , IL 6 , IL 8 , IL 10 , IL 12 alpha , IL 12 beta , IL 15 , IFN alpha , IFN gamma , and TNF alpha share 93 to 99 % homology at the nucleic acid and protein level with the human equivalents . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We show that spleen cell proliferation to Ag and to T cell polyclonal stimuli is increased by neutralizing mAbs to IFN gamma , TNF alpha and beta , or by the inhibitor of NO synthase , NG monomethyl L arginine , added to the cultures . ^^^ Thus , the suppression of proliferative responses in T . cruzi infected mice resulted largely from increased NO production by macrophages activated by IFN gamma and TNF allied to insufficient IL 2 to fully support in vitro growth of T lymphocytes . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Rat and mouse fibroblasts were also found to produce nitric oxide when primed with IFN gamma and simultaneously treated with IL 1 , TNF alpha , or LPS . ^^^ Fibroblasts were also found to be distinct from macrophages in their sensitivity to the suppressive effects of transforming growth factor beta , which in fibroblasts inhibited both IFN gamma plus LPS and IFN gamma plus TNF alpha induced nitric oxide production . ^^^ At the stage of growth crisis , a dramatic increase in nitric oxide production was observed in rat fibroblasts in response to IFN gamma or TNF alpha that may be directly correlated with cellular senescence . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The aim of this study is to investigate whether regional infusion of tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) could improve the therapeutic results of hepatic artery ligation ( HAL ) on liver cancer in a rat model . ^^^ Animals were then divided into four groups of 10 each to receive seven daily intraarterial injections of IFN gamma 100 , 000 IU / rat / day ( HAL + IFN group ) , TNF alpha 30 micrograms / rat / day ( HAL + TNF group ) , IFN + TFN ( HAL + IFN + TNF group ) , or normal saline solution ( HAL group ) . ^^^ These data suggest that HAL combined with regional infusion of TNF alpha and IFN gamma significantly reduces tumor growth in a rat liver model . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : The sera from 9 patients with metastatic melanoma treated with MLV MTP PE , 4 mg i . v . twice a week for 12 weeks , were tested in ELISA system to detect sICAM 1 , TNF alpha , IL 6 , Interleukin 1 beta ( IL 1 beta ) and Interferon gamma ( IFN gamma ) before , and 2 and 24 h after the 1st , 12th and 24th infusion of MLV MTP PE . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Three patterns were observed : ( 1 ) IL 6 was elevated in doubly infected mice early after infection ( 7 days ) , but it declined at later times ( 19 days ) ; ( 2 ) IFN gamma , IL 1 beta , and IL 10 were elevated at both early and late times ; and ( 3 ) TNF alpha , IL 12p40 , and possibly TNF beta were elevated only at late times . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These results suggest that IFN gamma , but not TNF alpha or IL 1 beta , may in part participate in the pathogenesis of Dengue shock syndrome ( DSS ) frequently found in heterologously secondary DV 2 infections . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human retinal pigment epithelial ( RPE ) cells in culture respond to a mixture of cytokines ( IFN gamma , IL 1 beta , TNF alpha ) by producing large amounts of nitric oxide . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Effects of interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) association in intraperitoneal radioimmunotherapy : studies on an in vitro micrometastasis model . ^^^ Thus , i . p . radioimmunotherapy of micrometastases could be optimized by an initial injection of the IFN gamma TNF alpha combination . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We describe a novel stat related factor , p 93 , that is found in EGF treated A 431 cell extracts but appears to be absent in bovine fibroblast growth factor ( bFGF ) , IFN gamma , tumor necrosis factor alpha ( TNF alpha ) , and untreated cells . p 93 appears to be antigenically related to stat 91 . p185c neu+ , EGFr+ ( M 1 ) , and p185c neu kinase inactive , EGFr+ ( NEN 757 ) expressing cells undergo different mitotic responses to EGF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) alone induced an increased production of TNF alpha in RAW 264 . 7 cells , not further augmented by a subsequent exposure to silica of the IFN gamma treated cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , cytokines such as IFN gamma , TNF alpha , or growth factors such as bFGF , SCF and TGF beta were ineffective in modulating CD 44 expression . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The aim of this study was to investigate mechanisms of anti tumour activity and necrosis induced by combinations of tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) . ^^^ Treatment with TNF alpha and IFN gamma caused focal engorgement of tumour capillaries with erythrocytes , intravascular recruitment of polymorphonuclear cells and platelet adherence to the tumour vascular endothelium 4 h after the combined treatment . ^^^ Thus , the effect of the combined TNF alpha and IFN gamma therapy involved the selective destruction of the tumour vasculature , death of tumour cells and increased expression of a series of stromal cytokines , cytokine receptors and adhesion molecules , which could be implicated in the observed events . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) , potent inhibitors of haemopoiesis , enhance Fas receptor expression on bone marrow ( BM ) CD34+ cells , and both cytokines render haemopoietic progenitor cells susceptible to Fas mediated inhibition of colony formation due to the induction of apoptosis . ^^^ These results are consistent with the hypothesis that AA CD34+ cells , probably including haemopoietic progenitor cells , express high levels of Fas receptor due to in vivo exposure to IFN gamma and / or TNF alpha and are suitable targets for T cell mediated killing . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
For this purpose 5 7 days precultured ( medium RPMI 1640 + / 10 % fetal calf serum ) rat pancreatic islets were exposed for another 48 h to either culture medium alone or with addition of rat interferon gamma ( IFN gamma ; 1000 U / ml ) , or human tumor necrosis factor alpha ( TNF alpha ; 1000 U / ml ) or a combination of the cytokines . ^^^ The combination IFN gamma + TNF alpha caused a 5 fold increase in the medium nitrite accumulation , indicating induction of nitric oxide formation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The modulation of IL 6 and TNF alpha release by nitric oxide following stimulation of J 774 cells with LPS and IFN gamma . ^^^ Lipopolysaccharide ( LPS ) and interferon gamma ( IFN gamma ) induced nitric oxide synthase ( NOS ) activity , tumour necrosis factor alpha ( TNF alpha ) , interleukin ( IL ) 6 and prostaglandin ( PG ) E 2 release in J 774 cells , a murine macrophage cell line . ^^^ The NO donor S nitroso N acetyl penicillamine ( SNAP , 300 microM ) , significantly inhibited LPS and IFN gamma induced IL 6 release , but potentiated TNF alpha release . ^^^ These results indicate that NO can differentially modulate LPS and IFN gamma induced cytokine release from J 774 cells , up regulating TNF alpha but down regulating IL 6 , and that NO is cytotoxic to these cells . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma priming of monocytes enhances LPS induced TNF production by augmenting both transcription and MRNA stability . ^^^ Priming by IFN gamma was primarily manifested at the level of TNF mRNA accumulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha receptors were induced on HT 29 cl . 19A cells by a small non toxic dose of IFN gamma ( 5 U / ml ) . ^^^ After 4 h of the combined presence of TNF alpha ( 10 ng / ml ) and IFN gamma ( 5 U / ml ) , the tight junction structure was altered as shown by a significant decrease in the average strand number measured in the apico basal direction ( 5 . 50 + / 2 . 70 vs 3 . 73 + / 1 . 39 in control and treated cells respectively , P < 0 . 0001 ) and by a significant decrease in junctional depth ( 0 . 27 + / 0 . 14 and 0 . 17 + / 0 . 10 microns in control and treated cells respectively , P < 0 . 0001 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We conclude that initial immunostimulation by SEB which is indicated by a massive liberation of all interleukins measured ( IL 1 alpha , IL 6 , TNF alpha , IL 2 , IFN gamma , IL 4 ) is responsible for the growth restriction of L . monocytogenes in the organs of treated mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When used e . g . in conjunction with anti CD 2 antibodies or diacylglycerol derivatives , the in vitro stimulation of T cells with anti CD 28 mAb resulted within 36 h in high levels of CD 3 dependent cytolysis ( tested on a FcR+ target in the presence of anti CD 3 mAb ) and sustained lymphokine production , such as TNF alpha , IFN gamma and IL 2 , which may affect tumour growth when delivered locally by the transferred T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In multiple sclerosis ( MS ) and its animal model experimental autoimmune encephalomyelitis ( EAE ) the cytokines tumour necrosis factor alpha ( TNF ) , lymphotoxin alpha ( LT ) , and interferon gamma ( IFN gamma ) are of central pathogenetic importance . ^^^ Here , we report that rolipram , a selective type 4 phosphodiesterase inhibitor , stereospecifically suppresses the production of TNF / LT and less strongly also IFN gamma in human and rat auto reactive T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using RT PCR and Northern blot assays to quantify cytokine gene production and cytokine production from intact cells , we show that ( 1 ) rat MBP reactive T cells express IL 2 , IFN gamma , and TNF alpha as well as IL 5 and IL 10 ; ( 2 ) cytokine production is not an all or nothing phenomenon , but rather varies according to the type and dose of TCR ligand , leading to variation in the pattern of cytokine production , or dissociation between cytokine production and cell proliferation ; and ( 3 ) nonencephalitogenic T cells do not differ appreciably from their encephalitogenic counterparts in ability to produce major cytokines . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS AND RESULTS : We measured kinetics of secretion of tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) by mononuclear leukocytes from 8 control subjects , 10 patients with stable angina pectoris , and 10 patients with unstable angina pectoris . ^^^ TNF alpha and IFN gamma secretion were measured by ELISA . ^^^ The effect of TNF alpha and IFN gamma on superoxide radical generation by neutrophils was also examined . ^^^ Secretion of both TNF alpha and IFN gamma by mononuclear leukocytes increased progressively over 48 hours , and it was consistently higher ( P < . 02 ) in patients compared with control subjects . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The present study was performed to investigate the effects of interleukin 1 beta ( IL 1 beta ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) on the expression of inducible NO synthase ( iNOS ) and to measure high output production of NO by primary rat osteoblasts and osteoblastic cell lines ROS 17 / 2 . 8 , MC3T3 E 1 and MG 63 . ^^^ However , IL 1 beta and TNF alpha showed strong synergy with IFN gamma , but , surprisingly , lipopolysaccharide was found to exert potent inhibitory effects on IFN gamma induced nitrite synthesis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In line with this finding , we also show by using quantitative reverse transcription polymerase chain reaction analysis that SR 31747A increased LPS induced IL 10 mRNA accumulation in spleen cells , whereas the level of both TNF alpha and IFN gamma mRNA was dramatically decreased . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of mice with neutralizing anti mouse TNF alpha antiserum significantly increased LPS induced IL 10 plasma levels between 1 . 5 and 6 h but diminished those at 12 h , while circulating IL 6 , interferon gamma ( IFN gamma ) and granulocyte colony stimulating factor ( G CSF ) concentrations were attenuated overall , without a biphasic response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Data from EAE suggest that cytokines connected to the Th 1 phenotype of lymphocytes , especially IFN gamma but also TNF beta , TNF alpha and IL 12 , may promote inflammation while cytokines connected to the Th 2 subset , IL 4 , IL 10 and TGF beta , may potentially have a role in disease limitation . ^^^ It will be important to accurately study cytokines during immunotherapeutic interventions and in relation to immunogenetic variables in order to aim at immunotherapeutically intervening in the Th 1 , Th 2 balance as well as counteracting disease promoting cytokines such as IFN gamma and TNF alpha or promoting the action of downregulatory cytokines such as IL 10 and TGF beta . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although IFN treatment differentially modulates histamine secretion from PMC and IMMC , pretreatment of both MC types with IFN alpha / beta or IFN gamma inhibited their tumour necrosis factor alpha ( TNF alpha ) dependent cytotoxicity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
BMM treated with amoebic proteins and stimulated with LPS , or interferon gamma ( IFN gamma ) +LPS , resulted in a 33 % and 50 % reduction in TNF alpha mRNA levels , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We show that although the addition of exogenous tumor necrosis factor alpha ( TNF alpha ) does not potentiate the trypanocidal activity of IFN gamma in vitro , treatment of resistant C57BI / 6 mice with an anti TNF alpha monoclonal antibody increased parasitemia and mortality . ^^^ IFN gamma activated macrophages failed to kill intracellular parasites following treatment with 100 micrograms of anti TNF alpha . ^^^ We conclude that IFN gamma activated macrophages produce TNF alpha after infection by T . cruzi and suggest that this cytokine plays a role in amplifying NO production and parasite killing . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 6 , TNF alpha and IFN gamma mRNA were expressed non selectively in tumors , PBMC and normal rental tissue . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Rapid increase in plasma tenascin C concentration after isolated limb perfusion with high dose tumor necrosis factor ( TNF ) , interferon gamma ( IFN gamma ) and melphalan for regionally advanced tumors . ^^^ Nineteen patients were treated by isolation limb perfusion ( ILP ) with TNF , IFN gamma , melphalan ( 11 melanoma , 2 SCC and 1 STS ) , melphalan alone ( 3 melanoma ) or hyperthermia at 41 . 5 degrees C ( 2 melanoma ) . ^^^ ILP with TNF , IFN gamma and melphalan induced a rapid increase in plasma TN C levels , peaking in most patients between 24 or 48 hr after ILP . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increased expression of interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) in bone marrow failure disorders suggests a possible pathophysiologic role of these cytokines in disease . ^^^ In this study , we tested the action of TNF alpha and IFN gamma on phenotypically and functionally defined stages of hematopoietic development using highly purified progenitor cell populations assayed in standardized culture systems . ^^^ We hypothesized that the inhibitory effects of IFN gamma and TNF alpha might be related to the induction of programmed cell death . ^^^ In methylcellulose colony assays , IFN gamma and TNF alpha inhibited the growth of early hematopoietic cells , including committed CD34+CD38+ progenitor cells and phenotypically less mature CD34+CD38 cells , with 50 % decreased colony formation occurring in the range of 750 1 , 000 U / ml of IFN gamma and 10 15 ng / ml of TNF alpha . ^^^ More potent suppressive effects were observed in cultures supplemented with the combination of both cytokines than in cultures treated with IFN gamma or TNF alpha alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha enhanced the release of ET 1 in BCEC , and this enhancement was not modified by the simultaneous addition of interferon gamma ( IFN gamma ) . ^^^ BCSMC did not produce ET 1 , either spontaneously or in the presence of TNF alpha , IFN gamma , or of both together . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These HCV specific CTL were shown to produce cytokines including IFN gamma , TNF alpha , GM CSF , IL 8 , and IL 10 in an antigen and HLA class 1 specific manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Next , we show that , in the presence of monocytes , sCD 23 is a potent costimulator of IL 2 or IL 12 induced IFN gamma production by resting T cells in the absence of exogenous Ag and that this effect is partially reduced by anti TNF alpha mAb . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have previously demonstrated that high levels of inducible nitric oxide synthase ( iNOS ) expression in hepatocytes required a combination of LPS and TNF alpha , IL 1 beta , and IFN gamma . ^^^ Rat hepatocytes were stimulated in vitro with escalating doses of TNF alpha , IL 1 beta , or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A process regulated via IL 8 receptors by IFN gamma , TNF alpha , IL 4 , IL 10 , and IL 13 . ^^^ This process can be augmented by IFN gamma and TNF alpha , and inhibited by IL 4 , IL 10 , and IL 13 . ^^^ In addition , we also document that on T lymphocytes there exist IL 8 receptors that can be up regulated by IFN gamma , TNF alpha , and IL 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Eight groups of animals were studied , including 2 control groups ( mice only infected with A . fumigatus and those given cortisone acetate and infected with A . fumigatus ) and 6 groups of infected mice either treated or not treated with cortisone acetate and given IFN gamma , anti IFN gamma , TNF alpha , or anti TNF alpha . ^^^ No deaths occurred among the mice treated with IFN gamma and TNF alpha , compared with 40 % 80 % mortality in the other groups . ^^^ IFN gamma and TNF alpha treated mice also had fewer organs from which A . fumigatus could be cultured or demonstrated on histologic examination . ^^^ These observations suggest that IFN gamma and TNF alpha have protective roles in invasive aspergillosis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
THP 1 myelomonocytic leukemia cells cultured with either macrophage colony stimulating factor ( M CSF ) or interferon gamma ( IFN gamma ) alone produce , at best , only low levels of interleukin 1 beta ( IL 1 beta ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^ Cycloheximide treatment of the cultures containing M CSF and IFN gamma inhibited the production of IL 1 beta and TNF alpha . ^^^ Northern blotting studies revealed no effect of IFN gamma alone on IL 1 beta or TNF alpha mRNA production . ^^^ IL 1 beta and TNF alpha mRNA expression was observed at 2 and 6 h after treatment with M CSF or IFN gamma + M CSF . ^^^ Higher TNF alpha mRNA expression was observed at 2 and 6 h after treatment with IFN gamma + M CSF , and higher IL 1 beta mRNA expression was observed at 2 h after treatment with IFN gamma + M CSF compared with mRNA levels observed for cells cultured only with M CSF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The adherence of monocyte subset 27E10 , which is associated with inflammatory processes , increased after endothelial activation with interleukin 1 beta ( IL 1 beta ) , interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and the glucocorticoid prednylidene ( Pred ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In cultures with anti TNF alpha Abs ( polyclonal or monoclonal ) , a decrease in the rIL 2 dependent generation of IFN gamma was observed . ^^^ Recombinant TNF alpha , when added to rIL 2 supplemented cultures of LD lymphocytes , enhanced the rIL 2 induced generation of IFN gamma in a dose dependent manner . ^^^ TNF alpha appeared to up regulate rIL 2 induced IFN gamma production by LD lymphocytes and to modulate lymphokine activated killer cell generation ( neutralization of TNF alpha promoted cell expansion in LD lymphocyte cultures and cytotoxicity in CD16+ lymphocyte cultures ) . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Previously , we have reported an increase in the cytokines interleukin 2 ( IL 2 ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) early after left lung allotransplantation in dogs . ^^^ In contrast to IL 2 , TNF alpha and IFN gamma did not change in either BAL or plasma of the autograft . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , simultaneous administration of the same dose of TNF alpha with IFN gamma ( 1000 units daily ) resulted in a synergistic effects manifested by the retardation of tumor growth . ^^^ Addition of systemic infusion of M CSF to the local therapy with TNF alpha and IFN gamma induced further augmentation of antitumor efficacy and delayed progression of MmB 16 melanoma . ^^^ The strengthened antitumor effect of combination therapy including M CSF , TNF alpha and IFN gamma was most probably due to the increased release of monocytes from the bone marrow , their recruitment into the site of tumor growth and subsequent local stimulation of their antitumor activity . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Following activation with IFN gamma , the secretory activity [ nitric oxide ( NO ) and tumor necrosis factor alpha ( TNF alpha ) ] and surface marker expression ( la antigen ) of these Bcg ( Nramp ) ribozyme transfected macrophages were markedly lower than in activated control mock transfected macrophages ( B10R CTL ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of type A and B tumor necrosis factor ( TNF ) receptors on melanoma cells can be regulated by dbc AMP and IFN gamma . ^^^ Local administration of high dose r TNF alpha with IFN gamma in the limbs of melanoma patients has proved to be a very promising treatment . ^^^ The total TNF binding activity of cultured melanoma cells , as measured by binding of 125I labeled TNF alpha , was up regulated between 2 and 4 fold by incubation of cells with activators of protein kinase A or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have recently shown that the adhesiveness of cultured murine brain microvascular endothelial cells for lymphocytes can be increased significantly by pretreatment with IL 1 beta , TNF alpha , IFN gamma , and LPS . ^^^ The basal level of migration was up regulated significantly by treating the En / astrocyte monolayers with IL 1 alpha , IFN gamma , TNF alpha , and LPS . ^^^ Treatment of En cell / astrocyte monolayers with TGF beta 2 down regulated the level of leukocyte migration up regulated by IL 1 alpha , IFN gamma , and TNF alpha in vitro in a dose dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Drug activated lymphocytes secreted high amounts of IL 5 and normal or low levels of IL 2 , IFN gamma , IL 4 , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Since feline cytokine gene sequences other than TNF alpha were not available , mammalian DNA and mRNA sequences for IL 2 , IFN gamma , IL 4 , IL 6 , IL 10 , IL 12 and beta actin , obtained from the Genbank database were compared and oligonucleotide primers chosen from consensus sequences . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It has been recently reported that human pancreatic islets in tissue culture produce nitric oxide ( NO ) and show a decreased function when exposed for 6 days to combinations of cytokines ( interleukin 1 beta ( IL 1 beta ) + tumor necrosis factor alpha ( TNF alpha ) + interferon gamma ( IFN gamma ) . ^^^ Here we study the effects of nicotinamide ( Nic ; 10 or 20 mmol / l ) on these deleterious effects of cytokines ( 50 U / ml IL 1 beta + 1000 U / ml TNF alpha + 1000 U / ml IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Analysis of the interrelationship between IL 12 , TNF alpha , and IFN gamma production during murine listeriosis . ^^^ To evaluate the timing of IL 12 production , and its relationship to TNF alpha , and IFN gamma production during primary murine listeriosis , we measured cytokine mRNA and protein levels in C57B1 / 6 mice infected intravenously with Listeria monocytogenes ( LM ) . ^^^ P 40 mRNA ( like IFN gamma and TNF alpha mRNA ) only accumulated in animals infused with live , virulent bacteria . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study we compared LPS and IFN gamma induced transcription of the TNF alpha gene in two murine macrophage cell lines , ANA 1 and Pu 5 1 . 8 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mice infected with H . capsulatum and treated with neutralizing Abs to IFN gamma , TNF alpha , or IL 12 experienced accelerated mortality , indicating that endogenous production of these cytokines plays an important role in response to infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
As determined by reverse transcriptase PCR , IFN gamma and tumor necrosis factor alpha transcripts were present in trigeminal ganglia during both acute and latent HSV 1 infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Susceptibility of epithelial cells to infection by rhinovirus 14 ( but not rhinovirus 2 , an ICAM 1 independent strain ) can be increased by preexposure of cells to TNF alpha , whereas IFN gamma reduces susceptibility to infection by both rhinovirus strains . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PBMC that had internalized killed parasites but not PBMC incubated with T . gondii lysate antigens also stimulated preferential expansion and activation of gamma delta T cells as assessed by expression of CD 25 and HLA DR molecules . 5 gamma 9+V delta 2+ gamma delta T cells were cytotoxic for T . gondii infected cells in an MHC unrestricted manner , and produced IFN gamma , IL 2 , TNF alpha , but not IL 4 when incubated with cells infected with the parasite . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) produce greater relative ICAM 1 expression in basal keratinocytes than in more differentiated keratinocytes . ^^^ Basal keratinocytes show maximal induction of ICAM 1 expression due to innate sensitivity to TNF and IFN gamma stimulation , and to location adjacent to dermal sources of cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increased in vitro and in vivo tumoricidal activity of a macrophage cell line genetically engineered to express IFN gamma , IL 4 , IL 6 , or TNF alpha . ^^^ IFN gamma gene transfected J774A . 1 [ J 7 ( IFN gamma ) ] cells developed filamentous processes , increased doubling times , and enhanced tumoricidal activity against three tumor cell lines : the TNF sensitive fibrosarcoma line WEHI 164 and the TNF alpha resistant cell lines B 16 melanoma and C 1300 neuroblastoma . ^^^ Cytolytic J 7 ( IFN gamma ) cells showed upregulated expression of TNF alpha messenger RNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Powerful lymphocyte activators such as mitogens induced rapid cell proliferation together with the production of both inflammatory ( IL 1 alpha , IL 1 beta , IL 6 and TNF alpha ) and immune ( IFN gamma , TNF alpha and TNF beta ) cytokines , and immune activation markers ( soluble IL 2 receptor , neopterin and xanthopterin ) . ^^^ Candida induced an early burst of IFN gamma production on day 1 followed by later production of TNF alpha . ^^^ In cultures stimulated with PPD , both IFN gamma and TNF alpha were detected from day 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It is established that an approximately 70 kb Lcr plasmid enables Yersinia pestis , the causative agent of bubonic plague , to multiply in focal necrotic lesions within visceral organs of mice by preventing net synthesis of the cytokines tumor necrosis factor alpha ( TNF alpha ) and gamma interferon ( IFN gamma ) , thereby minimizing inflammation ( Lcr+ ) . ^^^ In this study , injected PAV suppressed TNF alpha and IFN gamma in mice challenged with avirulent 5 antigen deficient Y . pestis ( lcrV or Lcr ) and promoted survival in vivo of these isolates as well as salmonellae and Listeria monocytogenes ( with which the outcome was lethal ) . ^^^ Significant synthesis of TNF alpha and IFN gamma occurred in spleens of mice actively immunized with PAV after challenge with Lcr+ cells of Y . pestis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Gonadotrophin stimulated progesterone production is inhibited by interleukin 1 beta ( IL 1 beta ) , tumour necrosis factor alpha ( TNF alpha ) , or gamma interferon ( IFN gamma ) , the last two cytokine being more effective than IL 1 . ^^^ Although the three cytokines mentioned have little or no effect on viability of cultured luteal cells when administered separately , combined treatment with TNF alpha and IFN gamma results in a substantial decrease in the number of viable cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neutralizing antibody to IFN gamma did not change the elevated TNF level obtained in the blood when LPS was given after a muramyl peptide . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
By using in situ hybridization techniques , mRNA expression of TNF alpha , IL 2 , IFN gamma and IL 4 was determined . ^^^ Lymph node cells from RCII immunized animals generated a high amount of TNF alpha mRNA after restimulation with RCII , whereas restimulation with the mitogen Con A generated a cytokine mRNA response dominated by IL 2 and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 13 altered the synthesis of C 3 and factor B proteins also in fibroblasts stimulated with interferon gamma ( IFN gamma ) in addition to TNF , in the same direction as it did in cells stimulated with TNF alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Similarly , TGF alpha , interferon gamma ( IFN gamma ) , IL 6 , and tumour necrosis factor alpha ( TNF alpha ) substantially increased HaCAT cell IL 1 alpha , but had no effect on the IL 1Ra , with a concomitant reduction in the icIL 1Ra : IL 1 alpha ratio . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The functional immune responses of the patients were assessed as natural killer ( NK ) and lymphokine activated killer ( LAK ) cytotoxic activities and in vitro interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) synthesis . ^^^ Before transplant the synthesis of both IFN gamma and TNF alpha decreased following rIL 2 therapy , whereas higher levels of these lymphokines were found after posttransplant rIL 2 courses . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Therefore , the purpose of this study was to determine whether PAF , in comparison with IFN gamma and LPS , could stimulate macrophages for both TNF alpha and non TNF alpha mediated tumoricidal activity . ^^^ PAF , together with IFN gamma , also activated macrophages for tumoricidal activity against TNF alpha resistant tumor cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Exogenous bioactive TGF beta 1 inhibited NK cell DNA synthesis and production of IFN gamma , TNF alpha , and granulocyte macrophage CSF ( GM CSF ) by NK cultures consisting of > 98 % CD56+ cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inhibition of IL 10 expression by IFN gamma up regulates transcription of TNF alpha in human monocytes . ^^^ The Th 1 type lymphokine , IFN gamma , markedly up regulates TNF alpha production in monocytes . ^^^ We examined the effects of IFN gamma on IL 10 expression in LPS stimulated monocytes , and the relationship between IL 10 and TNF alpha production in these cells . ^^^ Thus , potentiation of TNF alpha production by IFN gamma in monocytes is coupled to inhibition of endogenous IL 10 expression . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We demonstrated previously that three proinflammatory cytokines , TNF alpha , IL 1 beta , and IFN gamma , as well as IFN gamma plus LPS , can enhance ICAM 1 expression by primary rat astrocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In an effort to elucidate the pathogenesis of this disease , we found that genes for inflammatory cytokines , including IL 1 alpha , IL 1 beta , IL 6 , TNF alpha , transforming growth factor beta 1 , IFN gamma , and IL 2 , as well as MHC genes were activated in transgenic joints . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Prior administration of neutralizing Abs to TNF alpha or IFN gamma had no effect on receptor shedding . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In mice , M . avium induces a Th 1 response leading to protective immunity dependent on IFN gamma and TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Administration of the TCR 5 beta CDR 1 peptide before murine retrovirus infection significantly prevented its suppression of splenic NK cell activity , T and B cell proliferation , and monokine ( IL 6 and TNF alpha ) and Th 1 cytokine ( IL 2 and IFN gamma ) release by splenocytes , and inhibited retrovirus induced elevation of Th 2 cytokine ( IL 5 and IL 10 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Production of granulocyte macrophage colony stimulating factor ( GM CSF ) , interferon gamma ( IFN gamma ) , interleukin 6 ( IL 6 ) , tumor necrosis factor alpha ( TNF alpha ) , and IL 4 was similar for TIL from melanoma responders and nonresponders , or TIL from RCC patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The measles virus specific T cells secrete large amounts of interleukin 2 ( IL 2 ) , gamma interferon ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) but no IL 4 , IL 6 , or IL 10 , and hence the cytokine pattern is consistent with that of subtype 1 T helper lymphocytes . ^^^ In contrast , cells obtained from measles virus infected susceptible C3H mice recognize measles virus proteins only weakly and secrete little IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha , and IL 1 alpha levels in the plasma were raised by 8 h to picogram levels per ml of plasma , whereas IFN gamma and IL 2 were raised by 2 h to nanogram levels per ml of plasma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 2 , IFN gamma , and TNF beta did not appear to be expressed at higher levels in TIL than in autologous or healthy normal PBL . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have assayed IFN gamma production in the supernatants from 16 paired TIL and PBL cultures , and tumour necrosis factor alpha ( TNF alpha ) in 6 paired cultures . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
LPS and IFN gamma both enhanced run off transcription of the TNF alpha gene ; however , LPS had a more pronounced effect . ^^^ IFN gamma resulted in secretion of TNF alpha in day 5 , day 7 , and day 9 BMDM after 4 8 hr of stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although IL 4 had a marginal protective effect , IL 1 , IL 3 , IL 6 , IL 8 , interferon gamma ( IFN gamma ) and IFN alpha , tumour necrosis factor alpha ( TNF alpha ) , transforming growth factor beta ( TGF beta ) and granulocyte macrophage colony stimulating factor ( GM CSF ) displayed no significant activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of gamma interferon ( IFN gamma ) and the priming for tumor necrosis factor production during an in vivo infection were similar between beige and immunocompetent C57BL / 6 mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
On both monocyte derived macrophages and the myelomonocytic cell lines , Fc alpha R / CD89 expression was induced by calcitriol alone and additively in combination with tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and granulocyte macrophage colony stimulating factor . ^^^ Fc epsilon RII was also triggered by IFN gamma , TNF alpha , and IL 6 on all the cell lines , an effect blocked by calcitriol . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Overt or unrecognized infection or inflammation is another common cause of hyporesponsiveness , and is a consequence of increased blood concentrations of cytokines such as tumour necrosis factor ( TNF ) , interleukin 1 ( IL 1 ) , and interferon gamma ( IFN gamma ) , which suppress erythrocyte stem cell proliferation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Spleen cells stimulated for 48 h with the disubstituted compounds produce immunoreactive interleukin ( IL ) 1 alpha , IL 6 , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) . ^^^ Monosubstituted analogues induce lower quantities of IL 6 , TNF alpha , and IFN gamma and fail to induce detectable levels of IL 1 alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In control subjects , MP and IL 2 were strong inducers of IFN gamma , IL 1 beta , TNF alpha , and GM CSF mRNA expression , but only MP was able to induce considerable levels of IL 6 and IL 2 mRNA expression . ^^^ In MP activated PBMC from glioma subjects , a highly defective IFN gamma , together with a significant reduction in TNF alpha and GM CSF mRNA expression , was observed . ^^^ The pattern of cytokine mRNAs in IL 2 activated PBMC of glioma patients confirmed the impairment of IFN gamma mRNA expression paralleled by a reduction in IL 6 , TNF alpha and GM CSF mRNA , compared with healthy subjects . ^^^ Coherently , in PBMC cultures from glioma patients , there was a clear cut decrease in the secretion of IL 6 and TNF alpha and especially of IFN gamma compared with healthy controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The growth of H37Ra in macrophages was increased in vitro by the neutralization of TNF and neutralization of either TNF and / or interferon gamma ( IFN gamma ) enhanced the in vivo proliferation of this microbe in the spleen and liver of infected animals , whereas only the combination of both anti TNF and anti IFN gamma enhanced bacterial proliferation in the lung . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
One such lipid , ceramide , generated from membrane sphingomyelin following stimulation with TNF alpha , IL 1 beta , or IFN gamma , activates ceramide activated kinase ( CAK ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 12 induced protection against blood stage Plasmodium chabaudi AS requires IFN gamma and TNF alpha and occurs via a nitric oxide dependent mechanism . ^^^ Administration of neutralizing mAb against IFN gamma or TNF alpha to IL 12 treated mice showed that simultaneous depletion of both cytokines resulted in 100 % mortality . ^^^ In conclusion , these results demonstrate that IL 12 regulates the development of resistance to P . chabaudi AS via a CD4+ Th 1 response , which involves the cytokines IFN gamma and TNF alpha , and is in part NO dependent . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The latter appeared significantly increased during acute infection whereas biologically active tumor necrosis factor was virtually undetectable in serum from infected rats regardless of whether they had been given IFN gamma or not . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Blast adhesion to hKEC was augmented by treatment with either interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 beta ( IL 1 beta ) , or MLR supernatant . ^^^ TNF alpha and IL 1 beta treatments on hKEC increased the susceptibility to LAK cytotoxicity , whereas IFN gamma and MLR supernatant treatment significantly reduced this susceptibility . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum cytokines such as interleukin 1 beta ( IL 1 beta ) , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) were measured in 40 patients with rheumatoid arthritis ( RA ) . ^^^ In the 40 patients studied , serum IL 1 beta was detected in 5 patients , IFN gamma in 10 patients , and TNF alpha in 20 patients . ^^^ These results indicated that serum IL 1 beta in RA patients reflects the activity of RA , while the serum IFN gamma and TNF alpha in RA patients may be related to circulating activated lymphocytes and monocytes , respectively . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the current study , we used reverse transcription ( RT ) polymerase chain reaction ( PCR ) to explore the mRNA expression of candidate inflammatory cytokines IL 1 alpha , IL 2 , IL 4 , IL 6 , TNF alpha , and interferon gamma ( IFN gamma ) in peripheral blood mononuclear cells ( PBMC ) and skin biopsies of allogeneic BMT patients with GVHD and controls . ^^^ These data suggest that upregulated expression of IL 2 , IL 4 , and IFN gamma may be part of the inflammatory cascade of human acute GVHD , while IL 1 alpha , TNF alpha , and IL 6 are not discriminatory for the inflammation observed at the time of initial GVHD diagnosis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Polymerase chain reaction ( PCR ) analysis of cytokine mRNA expression in the grafts revealed higher levels of IL 2 , IFN gamma , and IL 10 mRNA in grafts of diabetic compared with normoglycemic control mice , whereas IFN gamma and TNF alpha mRNA levels were significantly decreased in grafts of IL 4 plus IL 10 treated mice compared with either normoglycemic or diabetic control mice . ^^^ These results suggest that T helper ( Th ) 1 cells and their cytokine products ( IL 2 , IFN gamma , and TNF alpha ) may promote islet beta cell destructive insulitis and autoimmune diabetes recurrence in syngeneic islet transplanted NOD mice , and that administration of IL 4 plus IL 10 may inhibit diabetes recurrence by suppressing Th 1 cytokine production in the islet grafts . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The transport activity for cystine was enhanced when the cells were incubated with tumour necrosis factor alpha ( TNF alpha ) , but not with interferon gamma ( IFN gamma ) or interleukin 1 . ^^^ IFN gamma was rather repressive in the induction of the activity by LPS or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The earliest cytokine responses seen were increases in IL 2 , IL 10 , IFN gamma , MIP 1 alpha and TNF alpha in the spleen , suggesting a primarily Th 1 pathway . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin 1 alpha ( IL 1 alpha ) , tumor necrosis factor alpha ( TNF alpha ) , interferon alpha ( IFN alpha ) interferon gamma ( IFN gamma ) induce dThdPase activity in human cancer cell lines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A human hepatoblastoma cell line ( C3A , a subclone of HepG2 / C3 that is currently being used as a surrogate liver ) and human lung adenocarcinoma cells ( A 549 ) were stimulated with interleukin 1 beta ( IL 1 beta ) , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , or IL 6 to determine any differences in cell type responsiveness to individual cytokines for ICAM 1 upregulation . ^^^ Cytokine induced A 549 surface levels of ICAM 1 ( immunofluorescence flow cytometry , mAb R6 . 5 ) was highest for IL 1 beta ( peak levels similar to hepatocyte response ) , modest with TNF alpha ( peak levels less than hepatocytes ) , detectable with IFN gamma ( much less than hepatocytes ) , and nondetectable after IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Apoptosis could be induced by actinomycin D , anti fas antibody , IL 1 , and TNF alpha but not by IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Levels of TNF alpha , IL 1 alpha , IL 6 , IL 8 , and IFN gamma in acute phase sera were determined by use of ELISA and RIA assays . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After costimulation , CD4+ T cell cultures contained significant amounts of the Th 1 cytokines IL 2 , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) . ^^^ In CD8+ T cell cultures relatively more IFN gamma and TNF alpha but almost no IL 2 was measured after triggering of CD 3 and CD 26 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS Synovial fibroblasts were isolated from patients with osteoarthritis and stimulated with IL 1 beta , IL 4 , IL 6 , IL 10 , IL 12 , IL 13 , tumour necrosis factor alpha ( TNF alpha ) , and IFN gamma . ^^^ In functional adhesion assays , TNF alpha , IL 1 alpha and , to a lesser extent , IFN gamma led to increased adhesion of mononuclear cells , whereas IL 4 and IL 13 had no effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , tumor volumes in group 4 ( local treatment ) were significantly smaller ( P < 0 . 03 ; 5 + / 10 cm 3 , 8 + / 11 % GCAP+ cells ) as compared to the nontreated group , probably due to the presence of higher amounts of Ab and infiltrated activated T cells ( 567 + / 322 CD5+ cells / mm2 ) capable of secreting cytostatic cytokines like tumor necrosis factor alpha and IFN gamma as compared to groups 2 and 3 ( 266 + / 135 and 198 + / 86 CD5+ cells / mm2 , respectively ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Synergistic effect of IFN gamma and TNF alpha on expression of immune molecules and antigen presentation by Schwann cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study investigated the presence of mRNA coding for interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) , and interleukins 2 ( IL 2 ) and 6 ( IL 6 ) , in the mucosa of four coeliac patients in remission who had been challenged with either gliadin or synthetic gliadin oligopeptides . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Th 1 cells preferentially produce interleukin 2 ( IL 2 ) , interferon gamma ( IFN gamma ) , and tumor necrosis factor ( TNF ) , resulting in a cellular response that helps to eliminate infected cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Freshly isolated GC cells were examined for mRNA expression for IL 1 alpha , IL 1 beta , IL 2 , IL 3 , IL 4 , IL 5 , IL 6 , IL 10 , TNF alpha , and IFN gamma using reverse transcription polymerase chain reaction . ^^^ When the other nine cytokine mRNA were studied , freshly isolated CD57+ Th cells occasionally expressed mRNA for IL 10 , TNF alpha , and IFN gamma . ^^^ CD 57 Th cells were occasionally positive for IL 1 beta , IL 10 , IFN gamma , and TNF alpha , and negative for IL 2 and IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
VCAM 1 is upregulated in these cells by the cytokines TNF alpha , IL 1 , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Hepatocytes also express an inducible NOS following exposure to the combination of endotoxin ( LPS ) and tumor necrosis factor ( TNF ) , interleukin 1 ( IL 1 ) , and IFN gamma . ^^^ We found the mRNA for hepatocyte NOS to be a single band at approximately 4 . 5 kilobases which was maximally up regulated ( approximately 70 fold ) by the combination of TNF , IL 1 , IFN gamma , and LPS . ^^^ Combinations of two or three stimuli revealed strong synergy between TNF , IL 1 , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In a different situation , NK cells that were stimulated by TNF and Listeria products to release IFN gamma did not produce NO . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neutralizing antibodies ( antisera ) prepared against transforming growth factor beta , tumor necrosis factor alpha , interferon alpha ( IFN alpha ) , and IFN gamma did not affect the inhibitory effects of IL 4 on pure and mixed megakaryocyte colony formation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
HLA class 2 molecules were de novo expressed or enhanced by IFN gamma and TNF alpha . ^^^ The adhesion molecules ICAM 1 , LFA 3 , and VLA 2 were upregulated by IFN gamma , TNF alpha , and IL 1 beta , whereas melanoma associated antigens were hardly affected by cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) markedly increased iNOS mRNA levels in time and dose dependent manners . ^^^ Actinomycin D abolished the IFN gamma and TNF alpha induced increases in iNOS mRNA and nitrite production . ^^^ Cycloheximide , which abolished both the IFN gamma and TNF alpha induced increases in nitrite production , had no effect on the IFN gamma induced increase in iNOS mRNA but markedly inhibited the TNF alpha induced one . ^^^ These results suggest that IFN gamma directly induces the expression of the iNOS gene whereas TNF alpha mainly induces it via the induction of an intermediary protein in cultured VSMC . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have recently demonstrated that the adhesion of lymphocytes to cultured retroocular fibroblasts obtained from patients with Graves ' ophthalmopathy ( GO ) is mediated predominantly by the interaction of lymphocyte function associated antigen 1 ( LFA 1 ) , expressed on lymphocytes , with intercellular adhesion molecule 1 ( ICAM 1 ) , expressed by these cells following exposure to interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) , IL 1 alpha or purified thyroid stimulating immunoglobulins . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 4 down regulated and IFN gamma enhanced the TNF alpha and IL 1 beta induced increase in RANTES mRNA , whereas the induction of IL 8 mRNA by TNF alpha or IL 1 beta was inhibited by IFN gamma and augmented by IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Ag presentation to these T cell clones was enhanced by pretreatment of THP 1 cells with IFN gamma but not TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Experiments performed using epithelial cells cultured from renal tubules show that VCAM 1 is up regulated by addition of the inflammatory cytokines tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) . ^^^ Combination of TNF alpha and IFN gamma synergized to induce high levels of VCAM 1 expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our data demonstrate that : ( 1 ) these cells release NO when induced by IFN gamma and TNF alpha , ( 2 ) they constitutively secrete ET 1 , and ( 3 ) cAMP potentiates the cytokine induced NO release and exerts a biphasic regulation on ET 1 secretion : micromolar concentrations of 8 Br cAMP inhibit and higher doses stimulate ET 1 secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cultured human renal epithelial cells were treated with the cytokines IFN gamma and TNF alpha and alteration in expression and function of markers including Classes 1 and 2 MHC antigens and the adhesion molecules LFA 3 and ICAM 1 was examined . ^^^ It was found that a mixture of IFN gamma and TNF alpha induced Class 2 MHC antigens , up regulated expression of Class 1 , LFA 3 and ICAM 1 molecules and enhanced binding of 51Cr labeled mononuclear immune cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we evaluated the ability of HIV 1 specific cytotoxic T lymphocyte ( CTL ) clones derived from seropositive persons to release gamma interferon ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and TNF beta upon contact with target cells presenting viral antigen . ^^^ HIV 1 specific CTL clones directed at envelope , Gag , reverse transcriptase , and Nef epitopes specifically released IFN gamma , TNF alpha , and TNF beta upon contact with their relevant target epitopes but not following contact with irrelevant epitopes . ^^^ These studies indicate that in addition to mediating direct cytotoxicity , HIV 1 specific CTL may affect other immune responses by releasing IFN gamma , TNF alpha , and TNF beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MIF and IFN gamma synergize with lipid A to mediate migration inhibition but only IFN gamma induces production of TNF alpha and nitric oxide . ^^^ Distinct differences were also found between the mechanisms by which MIF and IFN gamma synergized with lipid A for activation in that IFN gamma primed and lipid A triggered macrophages produced TNF and nitric oxide ( NO ) , whereas MIF primed cells did not . ^^^ An inhibitor of NO production NG monomethyl L arginine inhibited MMI mediated by higher activating concentrations of lipid A and by IFN gamma primed and lipid A triggered macrophages , but had no effect on MIF primed cells in concert with lipid A for increased expression of both TNF alpha mRNA and NO synthase mRNA . ^^^ The activation process by IFN gamma to mediate migration inhibition appears to resemble requirements for rendering macrophages tumor cytotoxic in the production of TNF for autocrine mediated NO generation by primed macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Primary cultures of rat proximal tubule and inner medullary collecting duct cells generated NO . on exposure to TNF alpha and IFN gamma . ^^^ Stimulation of kidney epithelial cells with TNF alpha and IFN gamma dramatically increased the level of inducible nitric oxide synthase mRNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
SDZCHH 380 coated peripheral blood and lymph node T cells and , in contrast to OKT 3 , induced minimal release of IL 2 , IL 6 , TNF alpha , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These include 10 copies of IFN gamma response element ; 3 copies of gamma activated site ; 2 copies each of nuclear factor kappa B , IFN alpha stimulated response element , activating protein 1 , and tumor necrosis factor response element ; and one 10 box . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We examined 10 different melanoma cell lines for cellular expression and release of ICAM 1 ( CD 54 ) and LFA 3 ( CD 58 ) and the influence of cytokines , including IFN alpha , IFN gamma and TNF alpha . ^^^ While IFN alpha has no effect on cellular ICAM 1 ( cICAM 1 ) expression , IFN gamma and to a lesser extent TNF alpha can effectively up regulate cICAM 1 . ^^^ Its release can effectively be inhibited by IFN gamma in all lines and by TNF alpha in one , while IFN alpha has no effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In situ hybridization performed at the beginning of tumor regression revealed the presence of mRNA for IL 1 alpha , IL 1 beta , and TNF alpha , but also the presence of cells , with lymphoid morphology , expressing IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our experiments suggest that both interleukin 1 beta ( IL 1 beta ) and interferon gamma ( IFN gamma ) must be present together to elicit a response whilst tumour necrosis factor alpha ( TNF alpha ) augments this . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 2 or TNF alpha alone had no effect either on gene expression or enzyme activity , whereas IFN gamma had only modest activity . ^^^ When IL 2 or TNF alpha were used in combination with IFN gamma , there was a marked cooperative induction of both mRNA and enzyme activity . ^^^ When IL 2 and TNF alpha were added at saturating doses in the presence of IFN gamma , there was an additive effect on NOS mRNA expression suggesting that IL 2 and TNF alpha cooperate with IFN gamma through at least partially distinct intracellular signaling pathways . ^^^ Expression of NOS mRNA in response to IFN gamma / IL 2 or IFN gamma / TNF alpha treatment required protein synthesis , suggesting that cooperative cytokine induction of NOS involves the intermediate expression of new gene products . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Activation of HUVEC with interleukin 1 beta ( IL 1 beta ) or tumor necrosis factor alpha ( TNF alpha ) , but not with interferon gamma ( IFN gamma ) , generated the augmentative basal adhesion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CD 40 ligand transfected cells provided a potent costimulus for monocyte TNF alpha and IL 6 production in the presence of GM CSF , IL 3 , or IFN gamma , and enhanced IL 8 production stimulated by GM CSF or IL 3 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These cells were examined by the reverse transcriptase polymerase chain reaction ( RT PCR ) method for their expression of mRNA coding for the following 12 cytokines : IL 1 beta , IL 2 , IL 3 , IL 4 , IL 5 , IL 6 , granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , macrophage colony stimulating factor ( M CSF ) , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and leukemia inhibitory factor ( LIF ) . ^^^ Although mRNA coding for every cytokine was detected in decidual mononuclear cells , mRNAs coding for only G CSF , GM CSF , M CSF , TNF alpha , IFN gamma , and LIF were detected in CD 16 CD56bright NK cells . ^^^ Also , the supernatant of CD 16 CD56bright NK cell cultures was found to contain G CSF , GM CSF , M CSF , TNF alpha , IFN gamma , and LIF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our results showed that IL 1 beta , tumor necrosis factor ( TNF ) alpha , and MECIF activity induced the expression of ELAM 1 on HEC membrane while transforming growth factor beta ( TGF beta ) , IL 6 , and gamma interferon ( IFN gamma ) showed no effect . ^^^ MECIF activity , TNF alpha , and IL 1 beta significantly increased prostacyclin secretion whereas TGF beta , IL 6 , and IFN gamma showed no significant effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of ICAM 1 on the endothelium exposed to TNF , IL 1 , LPS and IFN gamma increase . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have examined the time course of appearance of mRNA for nitric oxide synthase ( NOS ) , intracellular nonheme iron nitrosyl complexes ( NHFeNO , detected by EPR spectroscopy ) , and rates of medium appearance of NO 2 + NO 3 in cultured rat and human hepatocytes stimulated with a combination of cytokines ( TNF alpha , IFN gamma , IL 1 beta ) and LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These TNF alpha mediated effects were enhanced by addition of IFN gamma to the cultures . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin 10 ( IL 10 ) has been reported to inhibit nitric oxide ( NO ) synthesis and microbicidal activity of interferon gamma ( IFN gamma ) stimulated macrophages ( M phi ) by preventing the secretion of tumor necrosis factor alpha ( TNF alpha ) which serves as an autocrine activating signal . ^^^ We have examined the effects of recombinant IL 10 on the capacity of IFN gamma together with exogenous TNF alpha to induce NO synthesis by bone marrow derived M phi . ^^^ IL 10 further increased NO 2 production by M phi stimulated in the presence of optimal concentrations of prostaglandin E 2 , a positive modulator of M phi activation by IFN gamma / TNF alpha . ^^^ Increased steady state levels of NO synthase mRNA were observed in 4 h IFN gamma / TNF alpha cultures and enhanced NO 2 ( ) release was evident 24 h but not 48 h after stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Western blot analysis showed that expression of p60src in monocyte derived macrophages can be enhanced if monocytes are differentiated into macrophages in the presence of interferon gamma ( IFN gamma ) , or tumor necrosis factor alpha ( TNF alpha ) . ^^^ Enhanced p60src expression caused by IFN gamma or TNF alpha correlated with an enhanced autophosphorylating kinase activity assayed in anti p60src immune precipitates . ^^^ The human monocytic cell lines , U 937 and HL 60 , induced to differentiate along the monocytic pathway by IFN gamma , or a combination of IFN gamma and TNF alpha , expressed higher amounts of the p60src , but not of the p59fyn or p62yes , kinase activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
DR antigen was induced by the presence of a mixture of IFN gamma , TNF alpha and TPA in 4 cases , but not in the other 4 cases . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MHC class 2 expression and lysis increased when astrocytes were coincubated with IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
More importantly , laboratory studies performed in conjunction with these clinical trials have provided fresh insights into the pathogenesis of MS by revealing immunoregulatory mechanisms in which endogenous IFN gamma , TNF alpha , and other cytokines appear to play central roles . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The IL 1 beta induced nitrite production by astrocytes was markedly enhanced when cells were co stimulated with IFN gamma or TNF alpha ( IFN gamma > TNF alpha ) ; LPS had no effect used as a single agent or in combination with other cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neither TNF alpha alone ( 10 to 2000 U / ml ) nor IFN gamma alone 25 to 500 U / ml ) showed significant effects on nitrite production . ^^^ In addition , simultaneous incubation of endothelial cells with IFN gamma plus IL 1 beta and / or TNF alpha led to near maximal NO production of endothelial cells , even at lowest IFN gamma concentrations ( 25 U / ml ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Similarly , in mice chronically infected with T . gondii and treated with anti IFN gamma mAb the resulting pathology was associated with decreased expression of TNF alpha and iNOS and increased expression of SAG 1 and SAG 2 . ^^^ Together these results suggest that reactivation of T . gondii results from a down regulation of IFN gamma and TNF alpha expression leading to decreased macrophage or microglial cell activation , release of parasite growth , and subsequent tissue damage . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
No activity for interleukin 1 alpha ( IL 1 alpha ) , IL 1 beta , interferon gamma ( IFN gamma ) , or tumor necrosis factor alpha ( TNF alpha ) , which are known to augment ICAM 1 expression , was detected in CEM SUP by ELISA . ^^^ In reverse transcriptase polymerase chain reaction ( RT PCR ) assay , CEM expressed a minimum amount of TNF alpha mRNA , but absolutely no IL 1 beta or IFN gamma mRNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Large amounts of tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) accumulated in serum of TGV beta 3 mice after injection of 10 ng SEA , whereas only marginal amounts were recorded in non TG even after injection of 100 micrograms SEA . ^^^ Reverse transcriptase polymerase chain reaction ( RT PCR ) analysis demonstrated that SEA induced TNF alpha and IFN gamma mRNA reached maximal levels 1 hr after SEA administration in TGV beta 3 mice , whereas peak serum levels of TNF alpha and IFN gamma proteins were recorded after 2 hr . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The ability of tumor necrosis factor alpha ( TNF alpha ) alone and in combination with gamma interferon ( IFN gamma ) to inhibit the growth of interferon sensitive and resistant Rickettsia prowazekii strains in mouse L 929 cells was examined , and the possible role of the nitric oxide synthase pathway in the suppression of rickettsial growth induced by TNF alpha , IFN gamma , or both cytokines was evaluated . ^^^ TNF alpha inhibited the growth of strains Madrid E ( IFN gamma sensitive and alpha / beta interferon [ IFN alpha / beta ] sensitive ) and Breinl ( IFN gamma sensitive and IFN alpha / beta resistant ) , but not that of strain 83 2P ( IFN gamma resistant and IFN alpha / beta resistant ) , in L 929 cells . ^^^ Inhibition of the growth of the Madrid E strain in L 929 cells treated with TNF alpha and IFN gamma in combination was greater than that observed with either TNF alpha or IFN gamma alone . ^^^ Similarly , inhibition of the growth of the Breinl strain in L 929 cells treated with both cytokines was greater than that observed with TNF alpha alone ; however , it did not differ significantly from the inhibition observed with IFN gamma alone . ^^^ Although strain 83 2P was resistant to TNF alpha or IFN gamma alone , its growth was inhibited in L 929 cells treated with TNF alpha and IFN gamma in combination . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human nasal mucosal samples exposed in vitro to substance P or allergenic Ag were tested for the mRNA of IL 1 , IL 2 , IL 3 , IL 4 , IL 5 , IL 6 , TNF alpha , and IFN gamma using specific reverse transcriptase polymerase chain reaction assays . ^^^ After the administration of substance P , at dosages ranging from 10 ( 6 ) to 10 ( 9 ) M , an enhanced expression of the mRNA for IL 1 beta , 3 , 5 , 6 , TNF alpha , and IFN gamma was observed in all mucosal samples of allergic subjects and in half of the nonallergic subjects . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The interaction also resulted in the accumulation of TNF and IL 6 , but not IFN gamma in the culture medium . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of interferon gamma ( IFN gamma ) and / or tumor necrosis factor alpha ( TNF alpha ) on the growth of leukemic blast progenitors in 6 acute myeloblastic leukemia ( AML ) patients , 1 chronic myelocytic leukemia ( CML ) patient in blast crisis and a granulocyte colony stimulating factor ( G CSF ) dependent OCI / AML1a cell line established from an AML patient , were studied . ^^^ The recovery of clonogenic cells in suspension culture was also suppressed by IFN gamma and TNF alpha . ^^^ Therefore , IFN gamma and TNF alpha are considered to be effective in suppressing not only the terminal divisions but also self renewal of leukemic blast progenitors . ^^^ The results may suggest the effectiveness of IFN gamma and TNF alpha in the treatment of leukemia . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The BP specific lines produced Th 1 lymphokines ( IL 2 , IFN gamma , and TNF alpha ) , while the spinal cord lymphocytes produced the same Th 1 lymphokines as well as IL 4 and IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Exposure of NK cells to IL 2 or of EC to IL 1 , TNF , or IFN gamma augmented transendothelial migration . mAb directed against CD 18 and CD11a inhibited binding and migration of NK cells across resting or IL 1 activated EC , whereas anti CD11b and c mAb did not . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
ICAM 1 is constitutively expressed on the endothelioma surface and expression is increased by TNF alpha , IL 1 , LPS , and IFN gamma . ^^^ Furthermore , although , preincubation of bEND . 3 cells with IFN gamma modestly increases the induction of ICAM 1 and VCAM 1 in response to TNF alpha and IL 1 , it dramatically reduces the TNF alpha , IL 1 , and LPS induced expression of MAdCAM 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Four of five lesion free biopsies from psoriatic patients had prominent cytokine mRNA expression compared with skin from normal donors ( particularly TNF alpha , IL 1 alpha , IL 1 beta , with lesser increases in IFN gamma and granulocyte / macrophage colony stimulating factor [ GM CSF ] ) , which was accompanied by aberrant adhesion molecule expression in the same four samples . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found that cell lines that are sensitive to TNF mediated cytotoxicity ( TMC ) produced NO in response to TNF and IFN gamma , but not in response to IFN alpha beta . ^^^ However , L 929 cells treated with LA , TNF , and IFN gamma produced twice as much NO as cells treated with TNF and IFN gamma only . ^^^ Northern analysis for cytokine inducible NO synthase ( NOS ) mRNA steady state levels indicated that TNF synergized with IFN gamma to induce increased accumulation of NOS mRNA . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN alpha and IFN gamma which had no effect alone , synergistically increased TNF production when applied together with the enzymes . ^^^ Additionally we demonstrate that after ingestion of milligram doses of the polyenzyme preparation ( as recommended for clinical use ) , PBMNC of healthy donors acquire the ability to produce TNF alpha , IL 1 beta and IL 6 when incubated ex vivo with IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Further studies investigated roles for macrophage colony stimulating factors , the cytokines IFN gamma and TNF alpha , of neutrophils , and of nitric oxide in the SCID mouse , but have failed to demonstrate an important role in vivo for any factor examined . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokines stimulated by TTP were IFN gamma , IFN alpha and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we investigated the effect of LPS , IFN gamma and TNF alpha in the regulation of cox 1 and cox 2 mRNA expression in PMA differentiated U 937 human macrophages . ^^^ LPS , but not IFN gamma or TNF alpha , caused the induction of cox 2 mRNA in a dose and time dependent fashion . ^^^ In IFN gamma primed macrophages , LPS stimulated a marked increase in the accumulation of cox 2 mRNA , whereas TNF alpha and IFN gamma induced a moderate level . ^^^ However , IFN gamma in combination with either LPS or TNF alpha induced a synergistic increase in the accumulation of cox 2 mRNA after 24 hours . ^^^ These results indicate that IFN gamma priming is required for full expression of cox 2 mRNA in response to LPS or TNF alpha stimulation and suggest that cox 2 mRNA is highly regulated by cytokines during macrophage activation . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Studying the effect of tat and / or PMA + PHA on mRNA expression of 14 cytokines ( IL 1 alpha , IL 2 , IL 4 , IL 5 , IL 6 , IL 8 , IL 10 , IL 12 , TNF alpha , TNF beta , GM CSF , TGF beta , IFN gamma and MIP 1 alpha ) illustrated differential effects . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Analysis of cytokines in concentrated BAL fluid revealed significantly increased levels of IL 5 , IL 2 , IL 1 , TNF alpha , IL 6 , IL 8 , and GM CSF , but not of IL 4 and IFN gamma at 18 h compared with those at C and at 10 min . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We studied the production of TNF alpha in spleens and bladders of mice after intravesical BCG or BCG / interferon gamma ( IFN gamma ) instillation . ^^^ Significant change of TNF alpha mRNA expression of spleens and bladders of C3H / He mice was observed after intravesical BCG instillation , although intravesical IFN gamma therapy 3 days after BCG instillation to maintain the activated state of monocyte / macrophage lineage cells did not show a significant change of TNF alpha mRNA , compared with that of BCG therapy alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
SGHEC expressed significant levels of intercellular adhesion molecule 1 ( ICAM 1 ; CD 54 ) which increased after stimulation with tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 beta ( IL 1 beta ) , or interferon gamma ( IFN gamma ) . ^^^ Vascular cell adhesion molecule 1 ( VCAM 1 ; CD 106 ) and E selectin ( CD62E ) were not detectable on unstimulated SGHEC but substantial levels were expressed after stimulation with either TNF alpha or IL 1 beta but not with IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Whereas the parasite lysate stimulated patient PBMC to produce a mixed Th1 / Th2 type cytokine profile , LeIF stimulated the production of interferon gamma ( IFN gamma ) , interleukin 2 ( IL 2 ) , and tumor necrosis factor alpha but not IL 4 or IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Primers for 7 different human cytokine , IL 1 beta , IL 6 , IL 8 , GM CSF , TGF beta 1 , TNF alpha and IFN gamma were used to analyze messenger RNA transcripts by polymerase chain reaction ( PCR ) . ^^^ TNF alpha and IFN gamma were not found in glioma cell lines and specimens . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We conclude that IL 10 may be useful in controlling those clinical manifestations of acute GVHD that arise as a result of the activities of proinflammatory cytokines such as IFN gamma and TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , the amounts of TNF alpha and IFN gamma mRNA among intraparenchymal pulmonary mononuclear cells remained elevated after hemorrhage despite therapy with IL 1Ra . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RNA in situ hybridization was performed using 35S labeled riboprobes of HIV , TNF alpha , IL 1 beta , IL 2 , IL 4 , IL 5 , IL 6 , IL 10 , INF alpha , IFN gamma , and TGF beta . ^^^ The expression of IFN alpha , IFN gamma , and TGF beta mRNA was most prevalent early in the disease ; peak expression of IL 4 , IL 5 , IL 6 , and IL 10 was seen during the intermediate stage , and peak expression of TNF alpha and IL 1 beta mRNA were seen in AIDS patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It has been shown that the presence of IFN gamma , with TNF plus specific enhancing antibodies , further augmented antiviral activity in vivo ( 13 ) . ^^^ This finding stimulated interest in examining IFN gamma modulation of TNF R as a factor in the antiviral activity of TNF . ^^^ The expression of TNF receptor ( s ) in TNF and / or IFN gamma exposed cells , both with and without HSV 1 infection , was therefore examined . ^^^ Exposure of L 929 cells to IFN gamma alone also induced TNF receptor expression in mock infected cells . ^^^ HSV 1 infection of L 929 cells resulted in a significant upregulation of TNF R expression which was reversed if the cells had been preexposed to IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Alternative splicing of HLA class 1 transcripts induced by IFN gamma and TNF in fibroblasts : release of soluble HLA class 1 heavy chain and an associate protein . ^^^ FS 4 fibroblasts were found to produce 37 kDa HLA class 1 heavy chain in response to IFN gamma or TNF in a time and dose dependent fashion , and a synergism between IFN gamma and TNF was observed . ^^^ Immunoprecipitation of IFN gamma or TNF induced FS 4 cell culture supernatants by mAb A1 . 4 revealed an additional 33 kDa protein in association with the 37 kDa heavy chain . ^^^ The 33 kDa protein appeared to be expressed in a 38 kDa form on the membrane of FS 4 cells induced by IFN gamma or TNF , as A1 . 4 immunoprecipitated the 38 kDa band in association with the 44 kDa transmembrane HLA class 1 heavy chain . ^^^ Northern blot analysis and S 1 nuclease protection assay suggested the existence of HLA class 1 heavy chain mRNA lacking exon 5 in IFN gamma or TNF induced FS 4 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Several cytokines , in particular tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) , have been shown to be responsible for pathological reactions which may lead to shock and death observed in infection with Gram negative bacteria and in response to endotoxins ( lipopolysaccharides , LPS ) . ^^^ The priming effect of BCG on IFN gamma production appears to be mostly due to its ability to increase TNF alpha production , which acts as cofactor with LPS induced IL 12 in inducing IFN gamma production , as shown by the ability of injection of TNF alpha and LPS ( 1 microgram / mouse ) , but not LPS alone , to induce IFN gamma production . ^^^ However , in addition to TNF alpha , other LPS induced cofactor ( s ) are required in cooperation with IL 12 to induce optimal IFN gamma production , because co injection of TNF alpha and IL 12 , sufficient to induce serum concentrations of both cytokines higher and more persistent than those obtained by injection of LPS , was not sufficient to induce IFN gamma production in vivo . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In thioglycollate elicited peritoneal macrophages from wild typy ( WT ) mice , TNF enhanced nitric oxide ( NO ) release in the presence of IFN gamma , though TNF alone was not effective . ^^^ In macrophages from IFN gamma R0 / 0 mice , which are not responsive to IFN gamma , TNF completely failed to stimulate NO release . ^^^ Reduced lethality of LPS correlated with a drastically reduced TNF alpha production in the IFN gamma R0 / 0 mice after BCG infection and LPS challenge . ^^^ The greatly reduced ability of BCG infected IFN gamma R0 / 0 mice to produce TNF alpha may be an important factor in their inability to resist BCG infection . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When we measured serum cytokine levels , IL 6 , interferon gamma ( IFN gamma ) , and TNF alpha levels were increased prior to the onset of acute GVHD . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Likewise , immune complexes inhibited TNF and IFN gamma production by SCID splenocytes cultured with heat killed L . monocytogenes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , the rejection site showed higher gene expression of IFN gamma , TNF alpha , and IL 10 whereas TGF beta expression was slightly higher in the progressing tumors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Synergistic anti herpes effect of TNF alpha and IFN gamma in human corneal epithelial cells compared with that in corneal fibroblasts . ^^^ In this study we compared how effectively the proinflammatory cytokines TNF alpha and IFN gamma could inhibit HSV 1 replication in human corneal tissue fragments and monolayers of epithelial cells and fibroblasts derived from intact corneas , and investigated the mechanism responsible for the inhibition . ^^^ Pretreatment of corneal tissue or cells derived therefrom with TNF alpha ( 50 U / ml ) and IFN gamma ( 5 IU / ml ) consistently induced a synergistic antiviral effect . ^^^ We conclude that the combination of TNF alpha and IFN gamma induces a synergistic antiviral effect in corneal cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Two ovarian cancer cell lines , 3AO and NIH : OVCAR 3 , were used in this study to assess the synergistic effect of TNF and IFN gamma in combination . ^^^ The result showed that these two ovarian cancer cell lines were all resistant to TNF and IFN gamma individually , but sensitive to the combined administration of TNF and IFN gamma . ^^^ Concentration of 1 10 10 ( 4 ) U / L of TNF and IFN gamma significantly inhibited 3AO and NIH : OVCAR 3 ovarian cancer cells ( P < 0 . 05 ) . ^^^ Administration of 1 10 10 ( 6 ) U / L TNF and IFN gamma could inhibit the growth of 3AO cells by 55 % and NIH : OVCAR 3 cells by 75 % ( P < 0 . 01 ) . ^^^ It suggests that in some ovarian cancer cells which resistant to TNF could be changed to TNF sensitive cells by IFN gamma . anticancer treatment higher antitumor activity could be achieved by combined administration of TNF and IFN gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Skin biopsies from untreated and MDT treated patients were examined for infiltrating cells and cells producing the cytokines TNF alpha , IFN gamma , and IL 1 beta using immunohistochemistry . ^^^ Biopsy specimens from untreated tuberculoid leprosy patients were characterized by the presence of cells producing TNF alpha , IFN gamma , and IL 1 beta and of subepidermal Langerhans cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The presence of CD4+ cells was found to be closely associated with mRNA expression for IL 2 , IL 3 and IL 4 , a 5 fold increase in expression of TNF alpha and GM CSF and a 25 fold increase in expression of IFN gamma and TNF beta mRNAs , and temporally coincided with the development of granulomatous lesions . ^^^ In vivo neutralization of TNF alpha and , to a lesser extent , IFN gamma resulted in abrogation of granuloma formation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We looked at the in vivo expression of different cytokines during Mycobacterium avium infection of mice and found a correlation between resistance and the level of expression of IFN gamma and TNF alpha . ^^^ The use of specific neutralizing antibodies in vivo led to the identification of IFN gamma and TNF alpha as protective cytokines acting at the effector level of resistance to M . avium and M . tuberculosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma , are currently perceived to be important for protection , others , particularly tumor necrosis factor ( TNF ) , are thought to be responsible for much of the tissue destruction associated with the disease . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , IFN gamma administration also resulted in the induction of erythema nodosum leprosum , a toxic syndrome associated with excess TNF alpha production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study we examined whether other cytokines , such as tumour necrosis factor alpha ( TNF alpha ) , interferon beta ( IFN beta ) , IFN gamma or granulocyte macrophage colony stimulating factor ( GM CSF ) , could affect the decidualization of human endometrial stromal cells in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here we report a synergistic effect between A beta and interferon gamma ( IFN gamma ) in triggering the production of reactive nitrogen intermediates and tumour necrosis factor alpha ( TNF alpha ) from microglia . ^^^ These findings suggest that A beta and IFN gamma activate microglia to produce reactive nitrogen intermediates and TNF alpha , and this may have a role in the pathogenesis of neuronal degeneration observed in ageing and Alzheimer ' s disease . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines can operate in different ways in MS and the animal model `` experimental allergic encephalomyelitis ( EAE ) . `` Interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , TNF beta , interleukin 1 ( IL 1 ) and IL 2 are important inflammation mediators within the MS plaque , whereas IFN alpha , IFN beta , transforming growth factor beta ( TGF beta ) and IL 10 exert mainly immunosuppressive functions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In accordance with other studies rebound lymphocytosis with a marked increase of CD 56 ( + ) cells and release of secondary cytokines such as TNF alpha , IFN gamma and IL 6 was observed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of the mRNA of the cytokines IL 1 beta , IL 2 , IL 4 , IL 5 , IL 6 , TNF alpha , and IFN gamma , the lymphocyte markers CD 4 and CD 8 , and the HLA class 1 molecule , beta 2 microglobulin ( B2MG ) in the liver tissue of 20 CH ( C ) cases and 9 CH ( B ) patients was investigated by the reverse transcription polymerase chain reaction ( RT PCR ) method . ^^^ In chronic viral hepatitis , IL 1 beta and TNF alpha appear to play a major role in immune responses and IL 2 , IL 4 , and IFN gamma seem to be associated with increased cytotoxic T cell response . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , our studies demonstrate that one of the mechanisms for the activity of IFN gamma in this system is via the autocrine activity of TNF alpha . ^^^ These data imply that PMN derived chemokines are regulated by not only proinflammatory cytokines , including IL 1 beta and TNF alpha , but also Th 1 and Th 2 derived cytokines , including IL 4 , IL 10 , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inhibitory effect of melatonin on production of IFN gamma or TNF alpha in peripheral blood mononuclear cells of some blood donors . ^^^ The purpose of this study was to examine the effect of melatonin on the production of interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) by peripheral blood mononuclear cells ( PBMC ) in culture . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation of mesothelial cells in a confluential phase with IL 1 beta , TNF alpha , IFN gamma and TGF beta did not result in an increase in CA 125 release . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Analysis of repeated biopsies from four IBM patients after prednisolone treatment showed no change in the proportions of TNF alpha , ICAM 1 , IFN gamma or Ki 67 positive cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , mast cells are able to produce a variety of cytokines such as interleukin 4 ( IL 4 ) , IL 5 , IL 6 , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) which are known to be intensively involved in modulating and directing inflammatory responses in the skin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , the effect of dexamethasone on ICAM 1 expression stimulated by IL 1 beta , TNF alpha and IFN gamma was observed . ^^^ Treatment with IL 1 beta , TNF alpha and IFN gamma dose dependently increased ICAM 1 expression on NCI H 292 cells . ^^^ Inhibitory effects were exerted by dexamethasone on ICAM 1 expression in cells stimulated by IL 1 beta and IFN gamma in a dose dependent manner , but not in cells stimulated by TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Exposure to supernatant fluids ( SNs ) from Ag stimulated spleen cells of i . d . , but not i . v . or i . m . , immunized mice activated inflammatory M phi for in vitro killing of schistosome larvae , through a mechanism dependent on both IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Surprisingly , IL 12 treated / vaccinated mice failed to demonstrate a significant increase in IFN gamma , TNF alpha , or nitric oxide synthase mRNA at the time of challenge infection when compared with vaccinated controls , but did , however , display significantly suppressed Th 2 cytokine mRNA production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Macrophages from gene deletion mutant mice lacking either the IFN gamma receptor or the TNF receptor 1 ( p 55 ) failed to produce IL 12 in vitro after stimulation with rIFN gamma and mycobacterial infection . ^^^ In vivo , IL 12 production was induced in spleens of immunocompetent mice early during M . bovis BCG infection but not in those of mutant mice lacking the receptors for IFN gamma or TNF . ^^^ Our results show that IL 12 production by macrophages in response to mycobacterial infection depends on IFN gamma and TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
By measuring cytokine production and through cytokine depletion experiments , Wu Hsieh showed that besides IFN gamma , TNF alpha was important in host defences against the intracellular pathogen , H . capsulatum . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Irradiation reduced drastically IFN gamma production by LAK , but not TALL 104 cells ; release of TNF alpha and GM CSF by either type of effector was inhibited by 10 % 50 % , depending on the stimulus . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have investigated the role of gamma interferon ( IFN gamma ) and tumor necrosis factor ( TNF ) in acquired resistance to C . neoformans . ^^^ Neutralization of either IFN gamma or TNF impaired resistance of immune BALB / cBy or C . ^^^ Simultaneous neutralization of IFN gamma and TNF further exacerbated infection . ^^^ Neutralization of IFN gamma or TNF also impaired resistance in immune lymphocyte infused SCID mice , resulting in significantly higher yeast burdens in brains of cytokine neutralized mice than in brains of controls . ^^^ Concurrent neutralization of IFN gamma and TNF rendered SCID recipients of immune cells equivalent to uninfused SCID mice with respect both to brain yeast burdens at 10 days and to survival . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human NK cells produce IFN gamma , TNF alpha , and granulocyte macrophage CSF when stimulated with susceptible target cells or through the CD 16 and CD 94 cell surface molecules . ^^^ IL 5 production in these cultures occurs only when NK cells are in an exponential growth phase , whereas IFN gamma , TNF alpha , and granulocyte macrophage CSF are produced also by stimulation of quiescent cells , although to a lesser extent . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The interferon stimulated response element and a kappa B site mediate synergistic induction of murine IP 10 gene transcription by IFN gamma and TNF alpha . ^^^ The present study investigates mechanisms involved in cooperation between IFN gamma and TNF alpha to promote transcription from the IP 10 gene in NIH 3T3 cells . ^^^ IFN gamma synergistically enhanced TNF alpha induced levels of IP 10 mRNA , whereas levels of JE ( MCP 1 ) or KC ( GRO / MGSA ) mRNA induced by TNF alpha were unaffected by IFN gamma . ^^^ The cooperation between IFN gamma and TNF alpha for induction of IP 10 mRNA was independent of de novo protein synthesis and mediated at least in part by increased transcription . ^^^ IFN gamma and TNF alpha independently activated nuclear factors capable of specific interaction with the ISRE and kappa B sites , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tenidap inhibited IL 6 production in a dose dependent manner when cells were stimulated with a combination of TNF alpha and IFN gamma . ^^^ It also inhibited the priming effect of IFN gamma , preventing the super induction of IL 6 production following subsequent stimulation with TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Some aspects of humoral and cell mediated immunity and the capacity of peripheral blood mononuclear cells ( PBMCs ) of fourteen elderly persons with idiopathic anorexia to produce several cytokines , such as tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 beta ( IL 1 beta ) , interleukin 6 and interferon gamma ( IFN gamma ) , were studied and the results were compared with those obtained in a control group of ten age matched , normal weight healthy subjects . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Recombinant ( r ) TGF beta 1 dose dependently suppressed MBP induced upregulation of the proinflammatory cytokines IFN gamma , IL 4 , IL 6 , tumor necrosis factor alpha , ( TNF alpha ) , TNF beta and perforin , but not of the immunosuppressive and probably advantageous IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We evaluated the in vitro cytotoxic effects of combined human tumour necrosis factor alpha ( TNF ) , human interferon gamma ( IFN gamma ) , melphalan ( L PAM ) and hyperthermia ( HTX ) on human melanoma cell lines using the crystal violet assay . ^^^ IFN gamma enhanced the response to TNF only of the TNF resistant A 2058 cell line , but there was no enhancement of the response to L PAM for any line . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation of splenocytes from SCID mice with IL 12 in combination with either TNF alpha or IL 1 beta resulted in production of high levels of IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The regulatory mechanisms so far known to control NO synthesis include cytokines ( induction of NO synthase by IFN gamma , TNF alpha , MIF and LPS , and down regulation by IL 4 , IL 10 and TGF beta ) , feedback inhibition by NO itself , inhibition by pretreatment with LPS and glycoinositol phospholipids and up regulation by lipophosphoglycan from the protozoa parasite , Leishmania major . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 12 stimulated blood lymphocytes to produce interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) , and this effect was augmented by co cultivation with monocytes or AM . ^^^ AM upregulated induction of cytotoxic lymphocytes was stimulated with IL 12 , and this effect was significantly abrogated by addition of antibodies against IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN alpha , IFN gamma and TNF alpha suppressed cell proliferation significantly . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Upon in vitro stimulation of TNF alpha ( IFN gamma + muramyl dipeptide ) normals ' and immunocompetent patients ' MO TNFR expression is decreased for the entire 18 h period during which secreted TNF alpha is produced , but immunoaberrant trauma patients ' Mphi increased their TNFR expression , while concomitantly producing both secreted and cell associated TNF alpha protein . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
On Day 0 , before the fifth CA injection , PEC expressed a high level of IL 2 and a low level of IL 1 beta mRNAs while genes coding for IL 4 , IL 5 , IL 6 , IL 10 , IL 12 , TNF alpha , and IFN gamma were not expressed and there was a high level of NK activity . ^^^ This secondary pathway , without IL 12 involvement in the development of TH 1 response , is probably the result of the ability of IL 2 , IL 1 beta , and TNF alpha to synergize in inducing IFN gamma synthesis by NK cells . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Enhancement of IL 1 alpha , TNF alpha , TNF beta , IL 6 , IFN alpha , and IFN gamma mRNA was seen in the spleens of loxoribine treated mice . ^^^ ELISA assays on the supernatants of loxoribine treated spleen cells demonstrated that IL 1 alpha , IL 6 , TNF alpha , and IFN gamma were all produced in a dose dependent fashion with TNF alpha produced first , followed by IL 6 and IFN gamma , and last by IL 1 alpha . ^^^ IFN alpha beta activity rose as quickly as TNF alpha , leveling off at 8 to 12 hr , and was supplanted by a later occurring surge of IFN gamma production . ^^^ IL 1 alpha , IL 6 , TNF alpha , and IFN gamma were also detected in the sera of mice injected with loxoribine . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Freshly isolated IEC from surgical specimens were cultured in the presence or absence of lipopolysaccharide ( LPS ) , interferon gamma ( IFN gamma ) , IL 1 beta or TNF alpha . mRNA was assessed by a specific RNAse protection assay which controlled for contaminating cell populations while protein secretion was measured by ELISA ( IL 1 ) or bioassay ( TNF and IL 6 ) . ^^^ LPS , IFN gamma or TNF alpha had no effect on spontaneous IL 6 production , and neither resulted in the secretion of IL 1 beta or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Zn2+ could also reduce the TNF alpha secretion of keratinocytes stimulated by IFN gamma or Ni2+ during 48 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OBJECTIVE : We have studied lymphocyte induced cytotoxicity and the production of interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) during coculture of thyrocytes and autologous lymphocytes from patients with Graves ' disease and from normal subjects . ^^^ IFN gamma and TNF alpha measurements were performed after 1 , 5 or 7 days ' coculture . ^^^ In coculture , PB lymphocytes secreted more IFN gamma and TNF alpha than intrathyroidal lymphocytes . ^^^ The PB lymphocyte induced cytotoxicity was not due to IFN gamma and TNF alpha alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Priming of microglia with IFN gamma or TNF alpha resulted in a dose dependent enhancement of O 2 release in response to PMA . ^^^ We also found that IFN gamma and TNF alpha stimulated the release of bioactive TGF beta and that treatment of microglial cell cultures with TGF beta antagonized the priming effects of IFN gamma and TNF alpha on O 2 production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Similar patterns of IL 1 , IFN gamma and TNF alpha were seen while more IL 2 , IL 4 , IL 5 , and IL 10 mRNA was noted in large intestinal IEL . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Local and regional participation of IFN gamma , IL 10 , and TNF . ^^^ Using neutralizing Abs against IFN gamma , IL 10 , and TNF alpha / beta , we examined effects on GR size , GR macrophage function , and regional lymph node ( LN ) responses . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effect of ST 899 , a novel platelet activating factor ( PAF ) receptor antagonist , on serum tumor necrosis factor ( TNF ) , interleukin 6 ( IL 6 ) , and interferon gamma ( IFN gamma ) production as well as on lethality in an experimental endotoxin shock model was investigated in C57BL / 6 mice . ^^^ It is suggested that ST 899 may be beneficial , in combination with current therapies , in the treatment of diseases that involve overproduction of PAF , TNF , and IFN gamma such as septic shock . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Selective inhibition of spontaneous pulmonary metastasis of Lewis lung carcinoma by 5 ' deoxy 5 fluorouridine . 5 ' deoxy 5 fluorouridine ( 5 ' FUdR ) is a cytostatic that is biotransformed to 5 fluorouracil ( 5 FUra ) by pyrimidine nucleoside phosphorylase ( PyNPase ) , the expression of which is up regulated by tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 alpha ( IL 1 alpha ) and interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neither TNF alpha , IL 1 alpha nor IFN gamma was detected in the serum of mice bearing either clone , while IL 6 was detected in mice bearing both clones by ELISA and a bioassay . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The HTac antibody produced dramatic reductions in proliferation ( 27 % ) and in the secretion of cytokines ( TNF ) alpha and beta and IFN gamma inhibited 68 % , 72 % , and 80 % , respectively ) with no decrease in LAK . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CTL modified by 5 ' FSBA also failed to secrete TNF alpha and IFN gamma after activation by the appropriate Ag . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vivo blood monocyte migration to acute inflammatory reactions , IL 1 alpha , TNF alpha , IFN gamma , and C5a utilizes LFA 1 , Mac 1 , and VLA 4 . ^^^ Monocyte adhesion to cytokine ( IL 1 , IFN gamma , and TNF alpha ) stimulated endothelial cells was mediated by Mac 1 , LFA 1 , and VLA 4 , but Mac 1 appeared to be less important than LFA 1 or VLA 4 . ^^^ After i . v . injection , large numbers of 51Cr labeled blood monocytes migrated within 2 h to dermal inflammatory sites induced by C5a , IL 1 alpha , IFN gamma , TNF alpha , LPS , and poly inosinic : cytidylic acid . ^^^ Anti VLA 4 inhibited monocyte migration to IL 1 alpha , IFN gamma , TNF alpha , and LPS , but not to C5a . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Synergy between IFN gamma and taxol was mainly dependent on taxol induced TNF alpha secretion because not only the increase of inducible NO synthase ( iNOS ) gene expression by rIFN gamma plus taxol was associated with the increased expression of TNF alpha gene but also taxol induced NO production was decreased by the treatment of anti murine TNF alpha neutralizing Abs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma and TNF alpha induce redistribution of PECAM 1 ( CD 31 ) on human endothelial cells . ^^^ However , the effects of inflammatory cytokines such as TNF alpha and IFN gamma on endothelial PECAM 1 expression are not known . ^^^ We found that TNF alpha and IFN gamma produced dose dependent changes in the surface distribution of PECAM 1 , with a loss of the typical staining of PECAM 1 at intercellular junctions . ^^^ Because TNF alpha and IFN gamma did not alter PECAM 1 transcription or total surface PECAM 1 , these changes in PECAM 1 localization are most consistent with a redistribution of the protein away from intercellular junctions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The growth inhibitory effect by transfection induced TNF alpha was much remarkable as compared with exogenous TNF alpha and the effect was enhanced by the intraperitoneal injection of gamma interferon ( IFN gamma ) 12 h prior to intratumoral injection of the liposomes . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It was evident , however , that these in vitro expanded TIL expressing mRNA for TNF alpha and IFN gamma were qualitatively different and therapeutically less efficacious than the T cells associated with ISC or with freshly isolated TIL . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
LDA11 cells can be induced with lipopolysaccharide ( LPS ) , IL 1 alpha , or interferon gamma ( IFN gamma ) to express mRNA for monocyte chemoattractant protein 1 ( MCP 1 / JE ) , IFN inducible protein 10 ( IP 10 ) , stem cell factor ( SCF ) , and macrophage colony stimulating factor ( M CSF ) but not for IL 1 alpha , IL 3 , or tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin 2 ( IL 2 ) , IL 1 beta , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) , each alone and in all possible combinations , were studied for their capacity to activate murine resident splenic macrophages to a tumoricidal state . ^^^ Based on the utilization of neutralizing antibodies , IL 1 alpha , IL 1 beta , and IFN gamma were not involved as soluble mediators during the activation of tumoricidal splenic macrophages by IL 2 with or without TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We examined the interactive effect of several cytokines ( interleukin 1 beta [ IL 1 beta ] , tumor necrosis factor alpha [ TNF alpha ] , interferon gamma [ IFN gamma ] , IL 6 , IFN alpha / beta , and hepatocyte growth factor [ HGF ] ) presumably involved in hepatitis , on primary cultured murine hepatocytes . ^^^ The LDH release and DNA fragmentation induced by IFN gamma were inhibited by HGF in a dose dependent manner , whereas they seemed to be accelerated by TNF alpha . ^^^ Taken together , IFN gamma is presumed to be a critical cytokine in hepatic damage , and the network composed of IFN gamma , TNF alpha , and HGF may play an important role in the regulation of liver injury . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , freshly isolated blood monocytes express low levels of SP , which are , however , readily upregulated following exposure to IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , IL 4 downregulated TNF alpha and beta , IL 1 beta IL 6 and IFN gamma steady state mRNA and protein production by blood leukocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 10 inhibits the production of proinflammatory cytokines such as IL 1 , tumour necrosis factor alpha ( TNF a ) , interferon gamma ( IFN gamma ) and IL 6 through inhibitory action on Th 1 cells and macrophages , and it is thought to be a suppressor type cytokine . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytogenin enhanced productions of IL 1 alpha by macrophages and IFN gamma and GM CSF by spleen cells although it did not enhanced production of TNF alpha by macrophages and IL 6 by macrophages and spleen cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The preservation of anti chlamydial function after gamma irradiation correlated with the retention of IFN gamma and TNF alpha secretion in response to antigenic stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study the expression of tissue type plasminogen activator ( t PA ) , urokinase plasminogen activator ( u PA ) and plasminogen activator inhibitors 1 and 2 ( PAI 1 and PAI 2 ) antigen in conditioned medium from cultured human umbilical vein ( HUVEC ) and human saphenous vein ( HSVEC ) endothelial cells was investigated under basal conditions and after stimulation with LPS , TNF alpha , interferon gamma ( IFN gamma ) or interleukin 6 ( IL 6 ) alone or in combinations . ^^^ The effects of TNF alpha were modulated by IFN gamma but not by IL 6 . ^^^ The increased expression of u PA after stimulation with TNF alpha was reduced by IFN gamma . ^^^ In contrast , TNF alpha induced expression of PAI 2 was synergistically increased by addition of IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Reduced IFN gamma , IL 2 and TNF alpha production was associated with a significant decrease in PBMC proliferation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Supernatants from 20 URA patients with embryotoxic activity and IFN gamma , 13 reproductively normal parous women , and 10 men were further tested by ELISA for other TH 1 type cytokines ( interleukin 2 [ IL 2 ] , tumor necrosis factor beta [ TNF beta ] ) , TH 2 type cytokines ( IL 4 , IL 10 ) , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Since cytokines primarily appear to act locally at the tissue level , we analysed mRNA expression of several cytokines ( IL 1 alpha , IL 1 beta , IL 2 , IL 4 , IL 5 , IL 6 , IL 10 , IFN gamma , TNF alpha , TNF beta and TGF beta 1 ) in the lymph nodes of lupus prone mice , in models of early onset disease . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha , LT , IFN gamma and IL 6 mRNAs were detected in patients with active multiple sclerosis , whereas TNF alpha , IL 6 , and endothelin 1 mRNA expression was found frequently in patients with HIV encephalitis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of interleukin 12 ( IL 12 ) on natural killer ( NK ) cell cytotoxicity and on the production of interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) were examined in 15 patients with myelodysplastic syndromes ( MDS ) , which are well known to have immunologic defects , and in 11 normal subjects . ^^^ The combination of IL 12 and IL 2 augmented IFN gamma and TNF alpha production by nonadherent mononuclear cells in a synergistic or cumulative manner , respectively , in most patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The pattern of cytokine mRNA expression in frozen minor salivary gland tissues from patients with primary Sjgren ' s syndrome ( pSS ) ( n = 12 ) and controls ( n = 8 ) using an in situ hybridization technique and oligonucleotide probes of interleukin 1 beta ( IL 1 beta ) , tumour necrosis factor alpha and beta ( TNF alpha and TNF beta ) , interleukin 6 ( IL 6 ) , interleukin 2 ( IL 2 ) and its receptor ( IL 2R ) , interleukin 4 ( IL 4 ) , interleukin 10 ( IL 10 ) , interferon gamma ( IFN gamma ) and transforming growth factor beta ( TGF beta ) was examined . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Most noteworthy was a large number of clones from skin which secreted neither IL 2 nor IL 4 , but large amounts of tumour necrosis factor ( TNF ) and interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These results suggest that , in male mice , a rapid response to infection with high levels of TNF alpha and IFN gamma helps to control parasite multiplication , after which IL 10 production may be important in down regulating these potentially harmful inflammatory mediators . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ; 25 ng / ml ) exhibited a synergistic effect with IFN gamma by reducing the concentration of IFN gamma necessary to restrict intracellular growth to 100 U / ml . ^^^ The addition of 200 micrograms of tryptophan per ml significantly reversed the inhibitory effects of IFN gamma and TNF alpha , suggesting involvement of the indoleamine 2 , 3 dioxygenase pathway in the restriction process . . ^^^ Tumor necrosis factor alpha ( TNF alpha ; 25 ng / ml ) exhibited a synergistic effect with IFN gamma by reducing the concentration of IFN gamma necessary to restrict intracellular growth to 100 U / ml . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using U 937 cells , we demonstrate here that several cytokines [ interleukin 1 beta ( IL 1 beta ) , IL 4 , IL 6 , IL 13 , interferon gamma , tumor necrosis factor alpha ] could significantly up regulate the surface expression of CD 82 antigen , by contrast with FcR surface expression , which was up regulated only after IFN gamma treatments . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , no data are available on the relationship between GM CSF , TNF alpha and NO produced by macrophages activated by IFN gamma and infected with T . cruzi . ^^^ A high autocrine production of GM CSF or an exogenous supply of GM CSF is correlated with an enhanced release of TNF alpha and NO , inducing an improved control of T . cruzi infection by IFN gamma activated MPM . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum levels of tumour necrosis factor alpha ( TNF alpha ) , soluble interleukin 2 receptor ( sIL 2R ) , IL 1 alpha , IL 1 beta , IL 6 and interferon gamma ( IFN gamma ) were measured at baseline and at three monthly intervals for 15 months . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We show that the mRNA levels of c myc , c myb , c fos , tumor necrosis factors ( TNF ) alpha and beta , TNF receptors 1 and 2 , interleukin 1 alpha , IFN gamma , and transforming growth factor beta increase in the antisense transfected cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
No changes in the production of IL 1 beta , TNF alpha , IFN alpha and IFN gamma were detected following HIV infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
N acetyl L cysteine also suppressed keratinocyte surface expression of ICAM 1 induced by the cytokines interferon gamma ( IFN gamma ) or tumor necrosis factor alpha ( TNF alpha ) , whereas pyrrolidine dithiocarbamate and alpha tocopherol suppressed IFN gamma induced surface expression but not TNF alpha induced expression . ^^^ We found that N acetyl L cysteine treatment reduced ICAM 1 mRNA levels when keratinocytes were stimulated with either IFN gamma or TNF alpha ; however , pyrrolidine dithiocarbamate and alpha tocopherol had no effect on either IFN gamma or TNF alpha induced ICAM 1 mRNA levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The role of TNF alpha in the growth and differentiation of U 937 cells induced by PMA and IFN gamma ] . ^^^ The specific monoclonal antibody against TNF alpha diminished the effects of PMA and IFN gamma on the growth and differentiation of U 937 cells . ^^^ Thus the endogenous TNF alpha may involved in the mechanism of the effects of PMA and IFN gamma on the differentiation of U 937 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Induction of ICAM 1 by IFN gamma or TNF alpha on keratinocytes that do not express this molecule in normal skin may account for the recruitment of T cells into the epidermis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Recombinant IL 4 and IL 6 on their own suppressed the production of IL 1 beta , TNF alpha , IL 2 and IFN gamma by mononuclear cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
High titres of endogenous tumour necrosis factor ( TNF ) , interleukin 6 ( IL 6 ) and gamma interferon ( IFN gamma ) were induced in the bloodstreams and organs of the drug free mice . ^^^ DEX suppressed IL 6 production , but augmented TNF and IFN gamma production within 24 h of infection , whereas production of all three endogenous cytokines was suppressed in the DEX treated mice on day 3 of infection when the control mice began to die . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
As opposed to ruminants , TNF alpha has no effect in human luteal cells but potentiates the decrease of P 4 secretion induced by IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) , IFN alpha , IL 4 , IL 6 , and IL 7 , but not tumor necrosis factor alpha ( TNF alpha ) or IL 1 beta , induced a weak LAK activity against PCI 24 , whereas IL 2 induced ( 1000 U / mL ) LAK exhibited a far more potent cytotoxicity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study , we investigated the effect of 1 ) tumor necrosis factor alpha ( TNF alpha ) , a macrophage derived cytokine which promote the differentiation from monocytes to macrophages , and 2 ) interferon gamma ( IFN gamma ) , a T cell derived macrophage activating factor , on the production and mRNA expression of PDGF A and B chains in a human monocytic leukemia cell line THP 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The in vivo mechanism responsive to IL 12 was complex , and required both CD4+ and CD8+ T cells as well as natural killer cells and the action of multiple endogenous antileishmanial cytokines ( IFN gamma , IL 2 , tumor necrosis factor alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These included tumor necrosis factor alpha ( TNF alpha ) , granulocyte / macrophage colony stimulating factor ( GM CSF ) , interleukin 1 beta ( IL 1 beta ) and interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
As it is assumed that the T cell abnormalities are caused by specific interactions with the hairy cells , we studied the immunophenotype in 17 cases ( CD 3 , CD 4 , CD 8 , CD45R0 , TCR gamma delta ) and cytokine gene expression in four cases ( IL 1 beta , IL 2 , IL 3 , IL 4 , IL 6 , IL 7 , IL 10 , IFN gamma , TNF alpha , GM CSF and the receptors of IL 1 and IL 2 , using the cDNA PCR technique ) of purified T cell fractions from hairy cell spleens . ^^^ By Northern blot analysis , mRNA for IFN gamma , GM CSF , IL 10 and TNF alpha was measured in purified T cells and hairy cells from three HCL spleens . ^^^ Without specific induction spontaneous cytokine gene expression of IL 2 , IL 4 , IFN gamma , and GM CSF was seen in the purified T cell fractions without signals in the purified hairy cell fractions . mRNA expression of IFN gamma and GM CSF in the T cells , and of IL 10 and TNF alpha in the hairy cells was confirmed by the Northern blot technique . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin 10 ( IL 10 ) , originally identified as an inhibitor of cytokine and monokine synthesis [ e . g . , IL 2 , interferon gamma ( IFN gamma ) , and tumor necrosis factor ( TNF alpha ) ] , modulates a wide range of immunologic activities . ^^^ Induction of cytolytic activities by IL 10 was neutralized by anti IL 10 monoclonal antibodies but not by antibodies against IFN gamma or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These fractions contained interleukin ( IL ) 1 like activity which stimulated murine thymocyte proliferation . 100 150 U / ml human IL 1 beta , interferon ( IFN ) beta , IFN gamma and tumor necrosis factor ( TNF ) alpha stimulated the growth of both RPE cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
On the other hand , TNF alpha , but not other cytokines including IL 1 beta , IL 3 , IL 5 , IL 6 , interferon alpha ( IFN alpha ) or IFN gamma , inhibited IL 4 mediated growth , and inhibition by TNF alpha was blocked by anti TNF alpha Ab but not by control IgG . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of interleukin ( IL ) 1 beta , IL 4 , IL 6 , tumor necrosis factor ( TNF ) alpha , interferon ( IFN ) alpha , IFN gamma , and transforming growth factor ( TGF ) beta 1 on cytochrome P 450 ( CYP ) 1A expression and polycyclic aromatic hydrocarbon ( PAH ) mediated induction in primary human hepatocyte cultures were determined . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine mRNA levels for IL 2 , IL 3 , IL 4 , IL 5 , IL 10 and IFN gamma and functional cytokine secretion for IL 4 , IL 5 , IFN gamma , TNF alpha and mast cell differentiation activity were tested in vitro following stimulation with T . spiralis antigens . ^^^ However , analysis of the cytokine activities in culture supernatant of these T cell subsets following 24 h stimulation in vitro with T . spiralis antigens showed that significant IL 4 and IL 5 activity but little IFN gamma or TNF alpha was secreted by the protective CD4+ CD 45 RC cells . ^^^ Whereas the non protective CD4+ CD 45 RC+ cells secreted significant levels of IL 4 , IFN gamma , mast cell differentiating activity and TNF alpha but little IL 5 activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum levels of IFN gamma , IL 1 alpha , and TNF alpha all declined markedly by day 7 to 8 post BMT , when GVHD mortality begins . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Activation of PBM overnight with LPS , IFN gamma , or PMA resulted in secretion into the supernatant of TNF alpha , IL 1 beta , IL 6 , and IL 8 as assessed by ELISA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) or interferon gamma ( IFN gamma ) increased ScR mRNA levels , protein expression , and AcLDL degradative activity in cultured rabbit aortic smooth muscle cells . ^^^ Cytokines , such as TNF alpha or IFN gamma , may stimulate some of the phenotypic changes that characterize the alteration in gene expression of intimal smooth muscle cells in rabbit atherosclerotic lesions . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These CD4+ T cells released IFN gamma , IL 4 , and TNF alpha , but not IL 2 , in response to HLA DR15+ target cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here , we show that in an in vivo model of acute superantigen driven immune activation , CPZ independently down regulates the production of various T cell derived lymphokines ( IL 2 , IFN gamma , IL 4 , TNF , and GM CSF ) and up regulates the secretion of IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The inflammatory cytokines IFN gamma and TNF alpha have been demonstrated in various autoimmune diseases , and are thought to participate in the induction and pathogenesis of disease . ^^^ The expression of CD 3 , IL 2 , IFN gamma , and TNF alpha mRNA was barely detectable in the CNS of unmanipulated mice or mice that were immunized with adjuvant but showed no symptoms . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Maximal levels of interleukin 2 ( IL 2 ) , gamma interferon ( IFN gamma ) , tumor necrosis factor , IL 6 , and IL 10 were detected at the inflammatory site 7 to 10 days after infection , about the time that virus is cleared from the lung . ^^^ The frequencies of cells producing IL 2 , IL 4 , IL 6 , IL 10 , IFN gamma , and tumor necrosis factor were much higher for the bronchoalveolar lavage ( BAL ) cell population than for the MLN cell population . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vivo administration of neutralizing antibodies specific for gamma interferon ( IFN gamma ) or tumor necrosis factor alpha ( TNF alpha ) inhibited MHC class 2 induction on infiltrating M phi in both normal and CB 17 SCID mice . ^^^ The MCMV titer increased in the spleen and liver of IFN gamma depleted SCID mice , while TNF alpha depletion increased only splenic titers . ^^^ MCMV induced pathology was also increased in spleens of IFN gamma and TNF alpha depleted SCID mice . ^^^ We conclude that ( 1 ) M phi activation is a prominent part of inflammatory responses to herpesvirus infection and ( 2 ) IFN gamma and TNF alpha play a critical role in both virus induced M phi activation and control of herpesvirus growth independent of T and B cells . ^^^ This suggests that IFN gamma and TNF alpha mediated M phi activation is an important aspect of innate immunity to viral infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 6 and TNF were detectable in the PF and in the culture supernatants of PM phi whether stimulated or not by IFN gamma and LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Concomitant production of 19 different human cytokines , interleukin 1 alpha ( IL 1 alpha ) , IL 1 beta , IL 1 receptor antagonist ( ra ) , IL 2 , IL 3 , IL 4 , IL 5 , IL 6 , IL 8 , IL 10 , IL 13 , granulocyte macrophage colony stimulating factor ( GM CSF ) , G CSF , tumour necrosis factor alpha ( TNF alpha ) , TNF beta , interferon gamma ( IFN gamma ) and transforming growth factor beta 1 3 ( TGF beta 1 3 ) , was identified at a single cell level by indirect immunohistochemical staining procedures and use of carefully selected cytokine specific antibodies ( Ab ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mouse intestinal macrophages were purified , infected with MAC 101 , serovar 1 , and MAC 86 2686 , serovar 16 , and subsequently stimulated with recombinant tumor necrosis factor alpha ( TNF alpha ) , gamma interferon ( IFN gamma ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , or macrophage colony stimulating factor ( M CSF ) . ^^^ Stimulation with TNF alpha , IFN gamma , and GM CSF , but not M CSF , was associated with mycobacteriostatic and / or mycobactericidal activity in macrophages . ^^^ Treatment with 10 ( 3 ) U of TNF alpha , GM CSF , and IFN gamma per ml at 24 h prior to infection with MAC resulted in a significant enhancement in killing of MAC at 4 days after infection , compared with that observed for macrophages exposed to cytokines after infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Within 48 h , tumor necrosis factor alpha ( TNF alpha ) , interleukin 10 ( IL 10 ) , IL 12 , and gamma interferon ( IFN gamma ) mRNAs were expressed , and production of TNF alpha and IFN gamma was demonstrated . ^^^ After subcutaneous inoculation of heat killed LVS , no expression of any of the cytokine mRNAs and no increase in the levels of TNF alpha or IFN gamma occurred . ^^^ The expression of TNF alpha , IL 12 , and IFN gamma is held to be important to evoke an early T cell independent host defense against F . tularensis as well as to drive the expansion of a protective Th 1 cell response . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have developed a novel control fragment for multiple analyses of rat cytokine gene expression containing primers for IL 1 beta , IL 2 , IL 4 , IL 5 , IL 6 , IL 10 , TNF alpha , TGF beta 1 , IFN gamma and MIP 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Phenotypically distinct rat trophoblast cell lines , the Rcho 1 and R8RP . 3 cells , were compared for their responses to cytokines known to induce major histocompatibility ( MHC ) class 1 antigens , tumour necrosis factor ( TNF ) , transforming growth factor beta ( TGF beta ) , and interferon gamma ( IFN gamma ) . ^^^ Thus , phenotypically distinct rat trophoblast cell lines do not respond identically to TNF , TGF beta 1 or IFN gamma , transcription of cytokine genes does not prevent the cells from responding to paracrine cytokine signals , and the cells contain novel TNF transcripts that might be important in cell maturation or differentiation . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To examine the complex role of cytokines in the pathogenesis of actively induced murine EAE we measured the levels of a number of cytokines ( IL 6 , IFN gamma and TNF ) in the spinal cord and CSF of mice with active experimental autoimmune encephalomyelitis ( EAE ) and found them all to be elevated . ^^^ We next treated mice with antibodies to these three cytokines , which were over expressed in the CNS , to determine if they would alter disease and found the following : anti IL 6 had no significant effect on disease , anti IFN gamma exacerbated disease , and anti TNF either enhanced , had no effect or inhibited EAE depending on the antibody used . ^^^ We then treated mice with exogenous cytokines , delivered using a recombinant vaccinia virus system , and found that the IL 6 and TNF virus constructs inhibited EAE whereas the IFN gamma construct had no effect on disease . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To determine the cytokine synthesis inhibition that was critical for the IL 10 induced suppression of proliferation in MLR , the effect of exogenous cytokines ( IL 1 alpha , IL 1 beta , IL 2 , IL 4 , IL 6 , IFN gamma , or TNF alpha ) on this suppression was examined . ^^^ Investigation of the effect of IL 10 on cytokine synthesis revealed that when B cell lines were used as stimulators of MLR , IL 10 suppressed IFN gamma and IL 2 synthesis but was unable to suppress TNF alpha production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF , IL 1 , IFN alpha and IFN gamma decreased lipoprotein lipase activity and increased lipolysis in adipocytes . ^^^ TNF , IFN alpha and IFN gamma decreased fatty acid synthesis while IL 1 increased fatty acid synthesis . ^^^ Specifically , while all cytokines decreased LPL activity , only TNF and IFN gamma decreased LPL mRNA levels . ^^^ In addition , while TNF , IFN alpha and IFN gamma decreased fatty acid synthesis , only TNF significantly decreased the mRNA levels of both acetyl CoA carboxylase and fatty acid synthase , the key enzymes in fatty acid synthesis . ^^^ Finally , while all cytokines increased lipolysis , hormone sensitive lipase mRNA levels were decreased by TNF , IFN alpha and IFN gamma treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Co culture of B16F10 melanoma cells with IL 2 had no effect on nm 23 expression , whereas treatment with PGE 2 , TNF alpha and IFN gamma resulted in down regulation of nm 23 expression . ^^^ Concomitantly , in vivo treatment with TNF alpha or IFN gamma in experimental mice increased pulmonary metastases and lowered the overall survival period , as compared with IL 2 treatment alone . ^^^ The down regulation of nm 23 by TNF alpha , IFN gamma and particularly by PGE 2 warrants re examination of current immunotherapeutic protocols and of the role played by PGE 2 in tumor progression . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma induced production of sHLA was synergistically enhanced by IL 1 alpha , IL 6 or TNF , and combined treatment with TNF and IL 6 exhibited an additive to synergistic induction . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Addition of transforming growth factor beta ( TGF beta ) , interferon gamma ( IFN gamma ) , or tumor necrosis factor alpha ( TNF alpha ) to keratinocytes cultured with a 3T3 feeder layer did not induce suprabasal expression . ^^^ Injection of TNF alpha or IFN gamma intradermally into healthy human volunteers induced an inflammatory response but did not induce suprabasal integrin expression . ^^^ Addition of TGF beta , IFN gamma , or TNF alpha to the dermal equivalents neither induced nor inhibited suprabasal integrin expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study , TNF alpha , TNF beta and IFN gamma all inhibited thyroglobulin ( Tg ) and cAMP production from cultured human thyroid cells . ^^^ The hormones of the immune system , IL 1 , TNF and IFN gamma , may thus contribute to the decreased thyroid function characteristic of some thyroid inflammatory diseases . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found that ( 1 ) DMPP evoked [ 3H ] NE secretion was enhanced in SCG mixed cultures treated for 48 h with recombinant human TNF alpha ( rhTNF alpha ) plus rat interferon gamma ( IFN gamma ) but not in cultures treated with either cytokine alone ; ( 2 ) an increase in [ 3H ] NE secretion was also observed in mixed cultures treated with recombinant murine TNF alpha ( rmTNF alpha ) alone ; and ( 3 ) the presence of nonneuronal cells or soluble factors released by them was required for the effect of these cytokines on secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Total RNA was prepared from placenta of specific pathogen free BALB / c mice at the 16th day of gestation by acid guanidinium thiocyanate phenol chloroform ( AGPC ) method . cDNA was synthesized by M MLV reverse transcriptase , and amplified using the specific oligonucleotide primers for IL 1 , IL 2 , IL 3 , IL 4 , IL 5 , IL 6 , TNF alpha , IFN alpha , IFN beta and IFN gamma by PCR method . ^^^ IL 1 beta , IL 6 , TNF alpha , IFN alpha , IFN beta and IFN gamma mRNA were detected in all the placentas tested . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The anti leukemic effects of the crude preparation of IFN gamma are more intensive than those of the mixture of purified IFN gamma , IFN alpha , tumor necrosis factor alpha and lipopolysaccharide , suggesting existence of an unknown anti leukemic factor in the crude IFN gamma sample . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Total RNA was prepared from human spleen by acid guanidinium thiocyanate phenol chloroform ( AGPC ) method . cDNA was synthesized by M MLV RTase using oligo ( dT ) 16 primer , and amplified using the oligonucleotide primers specific for IL 1 alpha , IL 1 beta , IL 2 , IL 3 , IL 4 , IL 5 , IL 6 , IL 7 , IL 8 , IL 9 , TNF alpha , IFN alpha , IFN beta and IFN gamma by PCR method . ^^^ Although IL 1 beta , IL 4 , IL 5 , IL 6 , IL 7 , IL 8 , TNF alpha , IFN alpha and IFN gamma mRNA were detected in all the samples tested , IL 3 and IFN beta mRNA was not detected at all . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These cell lines secreted substantial amounts of IL 8 if stimulated with IL 1 beta , tumour necrosis factor alpha ( TNF alpha ) or interferon gamma ( IFN gamma ) , except CaCo 2 cells , which responded only to IL 1 beta . ^^^ In T 84 cells IFN gamma and epidermal growth factor ( EGF ) stimulated IL 8 secretion synergistically with TNF alpha , whereas in SW 620 cells this synergism occurred only between IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
C 4 expression was increased in a dose dependent manner by interferon gamma ( IFN gamma ) , but was unaffected by interleukin 1 beta ( IL 1 beta ) , IL 6 and tumor necrosis factor alpha ( TNF alpha ) alone , each of which augmented the expression of factor B , C 3 and other complement proteins synthesized in fibroblasts . ^^^ Simultaneous incubation of fibroblasts with IFN gamma and TNF resulted in a synergistic increase in C 4 synthesis . ^^^ RNA blot analyses indicated that regulation of C 4 synthesis by IFN gamma and the combination of IFN gamma and TNF was mediated primarily at a pretranslational level . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We show here , for the first time , the induction ( within 30 min ) of ICAM 1 gene expression in cultured normal human hepatocytes stimulated with interleukin 1 beta ( IL 1 beta ) , tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) or endotoxin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Time course studies over 30 days showed that sensitivity to TNF alpha was found in DTH sites of animals primed for IL 4 and IFN gamma production , but not in animals primed only for the Th 1 cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Incubation of human monocytic cells THP 1 with PLM led to dose dependent production of TNF alpha that was significantly increased by prestimulation of the cells with interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
While in vitro responses to secondary stimulation with SEB were reduced to background levels , the in vivo reactivity after rechallenge with SEB was retained , in that SEB primed mice succumbed to lethal T cell shock , lymphokines [ interleukin 1 ( IL 1 ) , IL 2 , Il 4 , IL 6 , IL 10 , interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) ] , and lymphokine specific mRNA accumulation could be detected in 5 beta 8+ CD4+ and 5 beta 8+ CD8+ T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Splenic natural killer ( NK ) cell activity , T and B cell proliferation , and T helper type 1 ( Th 1 ) and Th 2 cytokine ( IL 2 , IFN gamma , IL 5 and IL 10 ) and monokine [ IL 6 and tumour necrosis factor alpha ( TNF alpha ) ] secretions were monitored , as they are usually altered dramatically after murine retrovirus infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In order to study this we infected mice subcutaneously with vaccinia virus ( 10 ) constructs containing the genes encoding TNF alpha , IL 6 or IFN gamma and the density of LC , THY 1+ dEC and CD4+ cells was determined . ^^^ Production of TNF alpha during 10 growth did not influence this response by LC , whereas IFN gamma and IL 6 both increased the number of epidermal Ia+ LC at day 1 but then caused a reduction at day 5 . ^^^ However , by day 5 IFN gamma and IL 6 , but not TNF alpha , decreased the number of Thy 1+ dEC . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This assay had high throughput , did not require sterile conditions , could be carried out in the absence of serum , and was sensitive only to TNF and LT and not to interferon ( IFN ) alpha , IFN beta , IFN gamma , transforming growth factor beta , interleukin 4 , leukemia inhibitory factor and granulocyte monocyte colony stimulating factor ; all cytokines known to inhibit different cell types . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In our examination of the concomitant addition of each of 8 different recombinant human cytokines with 0 . 1 mM ferric citrate on SC DNA synthesis , 4 of them , i . e . , interleukin 1 beta ( IL 1 beta ) , IL 7 , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) each enhanced the synthesis of SC DNA at concentrations ranging from 1 to 100 u / ml , while IL 1 beta , IL 7 , TNF alpha or IFN gamma together showed an additive effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Other proinflammatory agonists , including TNF alpha , IFN gamma , and LPS failed to stimulate ENA 78 steady state mRNA or antigenic peptide production by renal cortical epithelial cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Additionally , effects of separated CD4+ and CD8+ activated lymphocytes as well as cytokine containing ( TNF alpha and IFN gamma ) supernatants from bMLC were studied . ^^^ High levels of TNF alpha and IFN gamma were found in the supernatant of allogeneic bMLC cultures , although when the supernatant was injected into the organotypic skin cultures , keratinocytes failed to express surface class 2 antigen and histologically did not show changes of skin GVHR . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , transformed human bronchial epithelial cells ( BEAS 2B ) were treated with interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , or with combination of these cytokines ( 10 ng / ml concentrations ) for 48 or 72 h and exposed to selected oxidants . ^^^ TNF alpha and IFN gamma + TNF alpha combination resulted in a marked increase of MnSOD protein and MnSOD activity . ^^^ When cells pretreated with the cytokines were exposed to hyperoxia ( 95 % O 2 , 72 h ) , menadione ( 5 50 microM , 4 h ) , or H2O2 ( 0 . 5 and 5 mM , 4 h ) , in all cases IFN gamma and TNF alpha enhanced oxidant related cell injury . ^^^ The effect was most significant with cells pretreated with a combination of IFN gamma and TNF alpha . ^^^ Catalase activity was not changed by IFN gamma or TNF alpha but it decreased significantly ( 34 % ) in IFN gamma + TNF alpha treated cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
All individuals studied regularly demonstrated high levels of transcripts for IL 1 beta , IL 6 and TNF alpha and transcripts for IFN gamma , TNF beta , IL 5 and IL 10 were variably expressed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , analog stimulated PBMCs secreted 59 % of the IL 1 beta , 25 % of the TNF alpha , and only 8 % of the IFN gamma produced in response to rIL 2 ( all p < 0 . 01 compared with rIL 2 stimulated secretion ; one way ANOVA ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In view of the potential uses of cell surface tumour associated antigens in novel anticancer treatment , a study was designed to investigate whether the biological response modifiers interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) could effect the expression of an epitope on the tumour associated MUC 1 epithelial mucin . ^^^ Four ovarian carcinoma cell lines showing high ( OAW 42 and GG ) and low ( JAM and PE 01 ) basal expression of MUC 1 were treated with 10 1000 U / mL of IFN gamma or TNF alpha for one or five days . ^^^ Changes in MUC 1 expression in cells exposed to IFN gamma or TNF alpha were monitored using an ELISA technique with the monoclonal antibody BC 2 which reacts with a core protein epitope on the MUC 1 mucin , and then corrected for the number of viable cells present . ^^^ TNF alpha had little effect on MUC 1 expression , but one or five days exposure to IFN gamma significantly increased MUC 1 expression ( p < 0 . 01 ) in all cell lines including the two cell lines that initially showed little or no expression . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Differentiated confluent cells were incubated with interleukin ( IL ) 1 , IL 6 , tumor necrosis factor , or interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The presence of these GM suppressor cells can be diminished by treatment of LLC LN 7 bearing mice with low doses of 100 units IFN gamma plus 10 units tumor necrosis factor alpha ( TNF alpha ) . ^^^ The aim of this study was to determine whether treatment of LLC LN 7 bearing mice with IFN gamma / TNF alpha to diminish GM suppressor cell presence would increase the responsiveness to IL 2 immune stimulatory therapy ( 100 1000 IU , twice daily for 5 days ) . ^^^ Treatment first with IFN gamma / TNF alpha and then also with low dose IL 2 increased both the numbers of CD4+ and CD8+ cells within the tumor and the levels of their expression of the p 55 IL 2 receptor . ^^^ Such effects were observed to a lesser extent in mice that were treated with either IFN gamma / TNF alpha alone or with low doses of IL 2 only . ^^^ The combination treatment regimen of IFN gamma / TNF alpha and then IL 2 was also significantly more effective at reducing the size of the primary tumor and the formation of metastatic lung nodules than were the individual treatments . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We assessed the ability of a number of recombinant human cytokines ( granulocyte macrophage colony stimulating factor ( GM CSF ) , interferon gamma ( IFN gamma ) , TNF alpha , IL 4 , IL 6 , transforming growth factor beta ( TGF beta ) , IL 10 and IL 1Ra ) ) to stimulate or inhibit the production of one or more of these monocyte products . ^^^ This was especially evident when cytokines were used in combination with GM CSF : IFN gamma down regulated production of IL 1Ra while up regulating the production of IL 1 alpha / beta , IL 6 and TNF alpha , while IL 4 had the exact opposite effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
All specimens were investigated for the distribution of staining for interleukin 1 alpha ( IL 1 alpha ) , interleukin 1 beta ( IL 1 beta ) , interleukin 6 ( IL 6 ) , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) and interleukin 2 ( IL 2 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our investigations concerning production of superoxide anion and TNF alpha by LPS and / or IFN gamma activated bone marrow and peritoneal macrophages , MHC class 2 expression on these cells , and the proliferative capacity and Il 2 production of mitogen activated lymphocytes , revealed that PTX reduces the activation and the inflammatory response of these cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1 beta , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , IL 6 , and IL 8 were used as stimulants . ^^^ HGF secreted IL 8 in a dose dependent manner after stimulation with either IL 1 beta or TNF alpha , but not with IFN gamma or IL 6 . ^^^ The combination of IL 1 beta and TNF alpha synergistically enhanced the secretion of IL 8 , whereas IFN gamma suppressed IL 8 secretion by IL 1 beta or TNF alpha stimulated HGF . ^^^ These results indicate that IL 8 production by HGF is synergistically stimulated by specific cytokines , IL 1 beta and TNF alpha , and suggest that these stimulatory effects are down regulated by IFN gamma at the transcriptional level through PGE 2 independent pathways . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The present study investigated changes in peripheral blood T cell surface markers and cytokines ( interleukin 2 , IL 2 ; tumor necrosis factor alpha , TNF alpha ; and interferon gamma , IFN gamma ) following tonsillectomy in patients with IgA nephropathy accompanied by chronic tonsillitis . ^^^ In some cases , the preoperative serum TNF alpha and IFN gamma levels were higher than normal before surgery , but decreased after surgery . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effect of TNF alpha on rickettsial growth in the mouse embryo cell line was not reproducibly enhanced by IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Rat specific oligonucleotide probes were used to semiquantitatively assess the relative expression of mRNA for TNF alpha , IL 1 beta , IL 2 , IL 6 , interferon gamma ( IFN gamma ) , and transforming growth factor beta 1 ( TGF beta 1 ) utilizing the reverse transcriptase polymerase chain reaction amplification technique . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It was observed that MO from mPDN treated mice were unable to produce sufficient levels of both TNF alpha and NO when stimulated in vitro with lipopolysaccharide , IFN gamma or conidia from an opportunistic fungus , Aspergillus fumigatus , confirming that corticosteroids are able to inhibit the antimicrobial activity of MO . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , our data indicate that IFN gamma and TNF alpha mediated alteration in the level of expression of distinct VLAs on ES and pPNET cells is correlated with changes in the adhesive behaviour of these tumour cells . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Among more than 100 random MLC experiments , we observed one cytokine pattern similar to the cytokine release detected in a control MLC of HLA identical siblings , which showed marginal or no secretion of IL 2 , sIL 2R , IFN gamma , TNF alpha , and IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Plasma TNF alpha was measured in 8 week old female non diabetic mice primed with 1000 units 4 of murine interferon gamma ( IFN gamma ) followed after 3 hours by 5 micrograms 4 of lipopolysaccharide ( LPS ) . ^^^ A separate group of female NOD / Lt mice had IFN gamma / LPS stimulated plasma TNF alpha measured at 10 weeks and were followed to age 30 weeks . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Upon stimulation by micro organisms , microbial products or endogenous factors including cytokines , macrophages can de novo synthesize and release a large variety of cytokines ( ie IL 1 , IL 1ra , IL 6 , IL 8 , IL 10 , IL 12 , TNF alpha , IFN alpha , IFN gamma , MCP 1 , MCP 3 , MIF , M CSF , G CSF , GM CSF , MIP 1 , MIP 2 , LIF , OSM , TGF beta ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Analysis of cells from alloantigen stimulated MLC revealed that UCB generated primarily CD4+ and CD16+ cells that made little or no IL 4 , IL 6 , TNF alpha or IFN gamma on antigenic stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Finally , the association of interferon ( IFN gamma ) with TNF alpha and / or glucagon was unable to modify the transport activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In both primary and secondary infection , the presence of CD4+ cells was a prerequisite for granuloma formation , and was found to be closely associated with mRNA expression for IL 2 , IL 3 and IL 4 , a 5 fold increase in expression of tumor necrosis factor ( TNF ) alpha and granulocyte macrophage colony stimulating factor , and a 25 fold increase in expression of IFN gamma and TNF beta mRNAs , suggesting a role for these cytokines in granuloma formation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We also noticed that the early stage group in NPC patients had higher levels of interleukin 1 alpha ( IL 1 alpha ) , TNF alpha , IFN gamma and sIL 2R in both spontaneous and PHA stimulated mononuclear cell supernatants . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To demonstrate the influence of n 3 PUFA supplementation on cytokine and eicosanoid production in peripheral blood mononuclear cells ( PBMCs ) of MS patients ( MSP ) , we investigated the impact of a 6 month dietary supplementation with these fatty acids on the levels of interleukin 1 beta ( IL 1 beta ) , IL 2 , interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) in the supernatants of stimulated PBMCs and serum soluble IL 2 receptors in a group of 20 relapsing remitting ( R R ) MSP and a group of 15 age matched control individuals ( CI ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In animal and in vitro models of mycobacterial infection , cytokines including tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and interleukin 1 beta ( IL 1 beta ) participate in granulomatous inflammation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neutralizing antibody to TNF alpha had a partial effect in combination with anti IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We studied huGRO , IL 8 and gamma IP 10 gene expression in unstimulated and stimulated ( TNF alpha , INF gamma , TNF alpha + IFN gamma , IL 1 beta , PMA and LPS ) normal human keratinocytes by Northern blot analysis . ^^^ The mRNA for none of the three chemokines was detectable in unstimulated keratinocytes , but considerably elevated levels of huGRO and IL 8 mRNA , but not of gamma IP 10 mRNA , were found in the presence of cycloheximide , indicating that huGRO and IL 8 mRNA , but not gamma IP 10 mRNA , are constitutively produced . gamma IP 10 mRNA was exclusively induced by IFN gamma , with a strong and transient rise between 8 and 18 h , and superinduced by the combination of IFN gamma and TNF alpha , indicating marked synergism . ^^^ Both huGRO and IL 8 mRNA were induced by TNF alpha and PMA ( a strong and transient rise between 2 and 8 h ) , but not by IFN gamma or LPS . ^^^ The combination of TNF alpha and IFN gamma did not show a synergistic effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Evidence exists for the role of tumor necrosis factor ( TNF alpha ) , granulocyte macrophage colony stimulating factor ( GM CSF ) and interferon gamma ( IFN gamma ) in the host defense against mycobacteria . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The importance of cytokines in resistance against T . gondii has been shown primarily in murine models of toxoplasmosis and a number of cytokines ( e . g . , IFN gamma , TNF alpha , IL 2 and IL 12 ) have been proposed for trials in patients with TE . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Regulation of TNF alpha promoter activity by IL 2 , IFN gamma , GM CSF , and IL 4 was examined in the U 937 macrophage cell line and the MLA 144 T cell line . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) have many effects on a number of cell types , including thyrotrophs . ^^^ In the present study , we used FRTL 5 cells , a cultured rat thyroid follicular cell line , to examine the effects of IFN gamma and TNF alpha on type 1 5 ' deiodinase ( 5 ' D 1 ) activity and 5 ' D 1 , thyroid peroxidase ( TPO ) and thyroglobulin ( Tg ) gene expression . ^^^ Incubation of FRTL 5 cells with the highest concentrations of TNF alpha and IFN gamma tested ( 1000 ng / ml or 1000 U / ml , respectively ) for 72 h in the presence and absence of TSH had no effect on cell viability as assessed by trypan blue exclusion . ^^^ In TSH deprived FRTL 5 cells , TNF alpha and IFN gamma resulted in a small but dose dependent decrease in 5 ' D 1 activity . ^^^ TNF alpha or IFN gamma blocked the TSH or cAMP induced rise in 5 ' D 1 activity . 100 ng / ml TNF alpha and 100 U / ml IFN gamma completely blocked the TSH or cAMP induced rise in 5 ' D 1 activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pretreatment of T cell reconstituted SCID mice with neutralizing anti IFN gamma monoclonal antibodies completely protected mice from bacterial challenge and reduced TNF alpha levels in serum . ^^^ We conclude that under the experimental conditions described here , IFN gamma and interleukin 2 producing TH 1 cells represent an important trigger mechanism inducing TNF alpha mediated hypersensitivity to bacterial endotoxin . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These CTL lines specifically lysed and release cytokines ( TNF alpha , IFN gamma , and GM CSF ) in response to T 2 cells pulsed with MART 1 ( 27 35 ) , as well as to HLA A2+ MART 1+ melanoma cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Regulatory mechanisms for production of IFN gamma and TNF by antitumor T cells or macrophages in the tumor bearing state . ^^^ Spleen cells from BALB / c mice bearing a syngeneic tumor ( CSA 1 M ) 2 to 3 wk after inoculation with CSA 1 M cells produced IL 2 , IFN gamma , and TNF upon in vitro cultures . ^^^ TNF was produced mainly by the non T cell fraction upon stimulation with CD4+ T cell derived IFN gamma . ^^^ Reciprocally to the progressive decrease in the production of IFN gamma / TNF , the capacities of tumor bearing mice to produce TGF beta and IL 6 increased along with tumor growth . ^^^ TGF beta suppressed production of IL 2 , IFN gamma , and TNF , but not of IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The kinetics of secretion of several cytokines ( such as TNF alpha , IL 1 beta , IL 6 , and IFN gamma ) , as well as expression of cellular activation markers , paralleled HIV replication in IL 2 stimulated PBMCs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Levels of the cytokines interleukin 1 alpha , 1 beta , and 2 ( IL 1 alpha , IL 1 beta , IL 2 ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) were measured in the mitogen stimulated whole blood cell cultures from 96 patients with Crohn ' s disease ( 48 untreated , 12 treated with sulfasalazine , 36 treated with corticosteroids ) , 74 patients with ulcerative colitis ( 21 untreated , 25 treated with sulfasalazine , 28 steroid treated ) , and 360 healthy controls . ^^^ In the blood cell cultures of the untreated and sulfasalazine treated patients with Crohn ' s disease and ulcerative colitis higher levels of TNF alpha , IL 1 alpha and IL 1 beta were found whereas IL 2 production was decreased and IFN gamma production was not significantly different as compared to the controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The majority of the T cell clones released IL 2 , IL 8 , TNF alpha , GM CSF but only a few IFN gamma and expressed high levels of mRNA for IL 2 , TGF beta and IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MAIN OUTCOME MEASURE : We compared the expression of tumor necrosis factor alpha ( TNF alpha ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , interferon alpha ( IFN alpha ) , IFN beta , and IFN gamma , and interleukin 1 alpha ( IL 1 alpha ) and IL 1 beta by reverse transcriptase polymerase chain reaction . ^^^ RESULTS : In patients with HAM , the reverse transcriptase polymerase chain reaction products of TNF alpha , GM CSF , IFN gamma , and IL 1 alpha were detected in significantly higher incidences than in HTLV 1 seropositive carriers and seronegative controls . ^^^ CONCLUSIONS : An exaggerated mRNA expression of several inflammatory cytokines , including TNF alpha , GM CSF , IFN gamma , and IL 1 alpha , was demonstrated in peripheral blood mononuclear cells of patients with HAM . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , all clones secreted at least small amounts of IL 2 , IL 4 , IFN gamma and TNF alpha , if stimulated by PMA and ionomycin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It is possible that the small subpopulation of EoL 1 cells which contains non specific esterase ( NSE ) activity may be preferentially differentiated by TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found a consistent profile of increased TNF alpha and decreased IFN gamma and IL 4 in all three syndromes compared to AIDS patients without neurological disease . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have recently reported that differentiation of myeloid cells , induced by phorbol ester , interferon gamma ( IFN gamma ) or colony stimulating factor 1 ( CSF 1 ) is accompanied by the activation of the differentiation induced factor ( DIF ) . ^^^ Antibodies raised against a Stat family protein , designated mammary gland factor Stat 5 ( MGF Stat 5 ) , reacted with DIF induced by either CSF 1 , GM CSF or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Involvement of IFN gamma in Bacillus Calmette Gurin induced but not in tumor induced sensitization to TNF induced lethality . ^^^ Using mice carrying a targeted disruption in the gene encoding the IFN gamma receptor ( IFN gamma Ro / o ) , we here report that endogenous IFN gamma plays a crucial role in the development of TNF hypersensitivity during BCG infection . ^^^ These results demonstrate that the enhancement of TNF effects is at least an equally important mechanism by which IFN gamma contributes to BCG induced hypersensitivity as the previously described augmentation of TNF production . ^^^ In contrast , IFN gamma Ro / o mice were as susceptible as control mice to the sensitizing effects of tumors . mTNF , which interacts with both mTNF R 55 and mTNF R 75 and causes lethality on its own , is as toxic in IFN gamma Ro / o mice as in wt control mice ; this means that TNF induced IFN gamma does not play a role in mTNF induced lethality . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Regulation by the cytokines IFN gamma , TNF alpha , and TGF beta . ^^^ The molecular mechanism ( s ) by which three cytokines ( IFN gamma , TNF alpha , TGF beta ) affect class 2 MHC gene expression in primary rat microglia was examined . ^^^ IFN gamma is a potent inducer of the class 2 gene , and this induction is unaffected by treatment with either TNF alpha or TGF beta . ^^^ Transient transfection of primary rat microglia with an HLA DRA promoter linked to the chloramphenicol acetyltransferase reporter gene ( DRA CAT ) demonstrated that IFN gamma acts at the transcriptional level to induce class 2 MHC gene expression , and that TNF alpha and TGF beta have no influence on IFN gamma induced promoter activity . ^^^ The effect of IFN gamma and TNF alpha on DNA binding proteins by microglia was examined . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It was found that mononuclear cells from cured individuals , in the presence of L . major promastigotes , had increased leishmanicidal activity and secreted increased levels of interferon gamma ( IFN gamma ) , interleukin 2 ( IL 2 ) and tumour necrosis factor alpha ( TNF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Since these cells produce cytokines such as tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) , we investigated the effects of these two cytokines on death of luteal cells in vitro . ^^^ METHODS : Mouse luteal cells were cultured in serum free medium with TNF alpha at 0 , 500 , 1 , 000 , 3 , 000 , or 5 , 000 U / ml in the presence or absence of IFN gamma at 1 , 000 U / ml for 3 or 6 days . ^^^ RESULTS : On day 3 of culture , IFN gamma alone at 1 , 000 U / ml or TNF alpha alone at 500 5 , 000 U / ml did not decrease the number of viable cells , but a combination of IFN gamma ( 1 , 000 U / ml ) and TNF alpha ( 5 , 000 U / ml ) did . ^^^ On day 6 , IFN gamma alone at 1 , 000 U / ml or TNF alpha alone at 500 , 1 , 000 and 3 , 000 U / ml did not decrease the number of viable cells , whereas TNF alpha alone at 5 , 000 U / ml did , and combinations of IFN gamma and TNF alpha at 1 , 000 , 3 , 000 , and 5 , 000 U / ml decreased the number of viable cells in proportion to the concentration of TNF alpha . ^^^ On days 3 6 of culture , combinations of IFN gamma and TNF alpha that decreased the number of viable cells also increased the percentages of fragmented DNA in total DNA of cultured luteal cells and the percentages of luteal cells with fragmented DNA in their nuclei . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin 1 ( IL 1 ) , tumour necrosis factor ( TNF ) and IFN gamma increased the cell surface expression of ICAM 1 and caused the release of soluble ICAM 1 from HOC cells established in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These cytokines include interleukins IL 1 beta , IL 2 , IL 4 , IL 6 , IL 8 , IL 10 , tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) and also soluble intercellular adhesion molecule ICAM 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study concerns the production of IL 1 beta , IL 2 , IL 4 , IL 6 , IL 8 , IL 10 , tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) and soluble ICAM 1 ( sICAM 1 ) throughout the six weekly instillations which comprise a therapeutic course . ^^^ Sequential instillations of BCG induced secretion of IL 1 beta , IL 2 , IL 6 , IL 8 , IL 10 , TNF alpha , IFN gamma and sICAM 1 into urine . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Production of tumor necrosis factor ( TNF alpha ) , interleukin ( IL ) 1 , IL 6 , IL 2 , and gamma interferon ( IFN gamma ) was observed . ^^^ Moreover , adding IL 10 to SE stimulated PBMC inhibited the production of IL 1 , IL 6 , TNF alpha , and IFN gamma by 50 to 80 % but had less effect ( 8 30 % ) on T cell proliferation . ^^^ The anti inflammatory agent , dexamethasone , was the most potent agent in controlling the SE mediated effects as evidenced by inhibited T cell proliferation ( 55 % ) and reduced levels of IL 1 , IL 6 , and IFN gamma ( 60 % to 100 % ) and TNF alpha ( 50 % ) . ^^^ Reducing levels of toxic mediators such as TNF alpha , IL 1 , IL 6 , and IFN gamma by dexamethasone in SE induced T cell responses may be a useful therapeutic strategy to circumvent SE toxicity and pathogenesis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It was found that LPS or IL 6 alone induced moderate levels of CAT expression , whereas IFN gamma or TNF alpha had no significant effect . ^^^ Co exposure of ms to IFN gamma and either LPS , IL 6 , or TNF alpha led to significant synergistic increases in CAT levels . ^^^ Levels were also synergistically augmented when ms were exposed first to IFN gamma ( priming ) , washed , and then exposed to either LPS , IL 6 , or TNF alpha . ^^^ LPS and TNF alpha were inactive when added before IFN gamma . ^^^ These results suggest that the duration of latency and progression of HIV infections may be greatly influenced by events such as intercurrent infections that cause IFN gamma production , thereby priming ms to respond to other cytokines that have been reported to be constitutively elevated during the course of infection with HIV ( e . g . , IL 6 and / or TNF alpha ) . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumour necrosis factor alpha ( TNF alpha ) and gamma interferon ( IFN gamma ) are expressed within human placental villi during normal pregnancy , yet their functions remain unknown . ^^^ Since villous cytotrophoblasts are within the paracrine reach of this expression , the effects of TNF alpha and IFN gamma on a purified population of term placental cytotrophoblasts were examined . ^^^ The combination of TNF alpha and IFN gamma enhanced the damaging effect . ^^^ After 24 h of culture , TNF alpha and IFN gamma increased the fraction trophoblasts containing nicked DNA , and after 60 h , increased the detachment of cells characterized by a distorted morphology , lower DNA content , and fragmented DNA . ^^^ These results suggest that a physiological role of TNF alpha and IFN gamma expression in the placental villi may be to regulate the apoptotic death of villous cytotrophoblasts . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Renal allograft rejection : the temporal relationship and predictive value of plasma TNF ( alpha and beta ) , IFN gamma and soluble ICAM 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Sex determined susceptibility and differential IFN gamma and TNF alpha mRNA expression in DBA / 2 mice infected with Leishmania mexicana . ^^^ Following reverse transcription , the polymerase chain reaction ( PCR ) was used to identify mRNA transcripts for interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) , interleukin 4 ( IL 4 ) , IL 10 and IL 12 . ^^^ Whereas all females expressed IFN gamma and one also expressed TNF alpha only two out of five males expressed IFN gamma and four out of five expressed TNF alpha . ^^^ The greatest lesion sizes at this time were recorded from those mice expressing TNF alpha but not IFN gamma . ^^^ At this time only transcripts for TNF alpha were present in males and only those for IFN gamma were detected in females . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The protective activity of IL 12 was abrogated by administration of monoclonal antibodies to IFN gamma or tumour necrosis factor alpha ( TNF alpha ) , and by depletion of NK cells using an antisera against asialoGM 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study we investigated the effects of tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) on the secretion of interleukin 6 ( IL 6 ) by myoblasts . ^^^ Purified human myoblasts from normal subjects and from patients with polymositis were cultured in the presence of TNF alpha and IFN gamma at two concentrations ( 100 and 200 U / ml ) , alone or in combination , for 12 , 24 and 48 h . ^^^ There was no synergism in the effect of IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma and TNF alpha cause an increased release of C 3 by murine macrophages . ^^^ Recombinant IFN gamma and TNF alpha were strong inducers of C 3 release , while IL 2 acted poorly . ^^^ The C 3 pro releasing activity of splenocyte supernatants was largely prevented by their pretreatment with specific mAb anti IFN gamma or anti TNF alpha , but not completely prevented by the simultaneous neutralization of the two cytokines . ^^^ Taken together , these results show that murine macrophages increase the release of C 3 molecules upon treatment with IFN gamma or TNF alpha and that these cytokines released in vivo by splenocytes from tumor bearing mice may account , together with a yet unknown factor , for a humoral network causing the increased release of C 3 by peritoneal macrophages . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This protective effect of anti IFN gamma antibody was associated with decreased IFN gamma levels in serum ; tumor necrosis factor ( TNF ) and interleukin 6 ( IL 6 ) levels remained unchanged . ^^^ Levels of TNF , IFN gamma , and IL 6 in serum were all more elevated in anti IL 6 treated mice than in corresponding SEB challenged control mice . ^^^ Higher levels of biologically active TNF and IFN gamma in serum were noted in these mice than in mice receiving SEB only . ^^^ Again , TNF levels in anti IFN gamma treated mice remained unchanged . ^^^ The data obtained with anti IL 6 antibody indicate that endogenous IL 6 is involved in regulating the levels of TNF and IFN gamma in serum . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The production and roles of endogenous gamma interferon ( IFN gamma ) , tumor necrosis factor ( TNF ) , and interleukin 6 ( IL 6 ) in both lethal and nonlethal infections of Staphylococcus aureus were investigated in mice . ^^^ The first peaks of IFN gamma , TNF , and IL 6 were observed in the bloodstreams and spleens of the mice with nonlethal and lethal infections within 24 h . ^^^ Thereafter , in the nonlethal cases , IFN gamma , TNF , and IL 6 peaked again in the spleens and kidneys during the period of maximum growth of bacteria in the kidneys , although only IL 6 was detected in the sera . ^^^ In contrast , in the case of lethal infection , the titers of IFN gamma and IL 6 in the sera and TNF in the kidneys peaked before death . ^^^ Effects of in vivo administration of monoclonal antibodies ( MAbs ) against IFN gamma and TNF on the fates of S . aureus infected mice were studied . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Levels of tumor necrosis factor ( TNF ) and gamma interferon ( IFN gamma ) in serum were , respectively , 10 and 50 fold higher in LPS potentiated mice injected with 15 LD50s of TSST 1 than in mice given H135A . ^^^ Mice injected with only TSST 1 did not have elevated levels of TNF or IFN gamma in serum , while H135A plus LPS or LPS alone elicited identical , yet very low , levels of TNF and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using in situ hybridization with radiolabelled cDNA oligonucleotide probes , we studied the expression of mRNA encoding the cytokines gamma interferon ( IFN gamma ) , interleukin 4 ( IL 4 ) , IL 6 , IL 10 , transforming growth factor beta ( TGF beta ) , tumor necrosis factor alpha ( TNF alpha ) , lymphotoxin , and perforin in mononuclear cells ( MNC ) from blood and cerebrospinal fluid ( CSF ) of patients with acute aseptic meningoencephalitis ( AM ) and from blood of healthy controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines such as IFN alpha , IFN gamma , G CSF , TNF alpha , IL 1 , and IL 4 markedly potentiate the differentiation inducing effect of retinoids . ^^^ On a series of human transformed epithelial cell lines a panel of cytokines , such as IFN alpha , IFN gamma , TNF alpha , TGF beta , and EGF acted synergistically with retinoids on inhibition of proliferation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human fetal astrocytes had transcript for IL 6 but not for IL 1 alpha or TNF alpha under basal culture conditions and following IFN gamma stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The intercellular adhesion molecule 1 ( ICAM 1 ) can be induced on many different cell types by a set of various modulators ( IL 1 beta , TNF , LPS , IFN gamma ) , which are released during the inflammatory process . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Alterations of cell surface expression of HLA ( class 1 , class 2 DR , DP and DQ ) and EGF receptor on two malignant glioma cell lines ( U 343MG and U 563MG ) induced with cytokines ( IFN gamma , TNF alpha , IL 1 alpha ) and differentiation promoters ( all trans retinoic acid , phorbol ester TPA ) were analyzed with the aid of flow cytometry . ^^^ TNF alpha alone induced a two to fivefold increase of HLA class 1 cell surface density and increased the IFN gamma induced upregulation of HLA class 1 to approximately 20 24 times the antigen density of uninduced cells . ^^^ TNF alpha was able to increase HLA class 2 DR and DP cell surface expression on glioma lines , but it enhanced only the IFN gamma induced HLA class 2 DR upregulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The loss of cytotoxicity correlated with down regulation of several adhesion molecule expressions on PE 9 cells , particularly the alpha beta TCR , as well as the mRNA for TNF alpha , IFN gamma and perforin , although the levels of granzyme A were not altered . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Homosexual males often present signs of immune activation and are likely to have increased levels of inflammatory cytokines such as IL 1 beta , TNF alpha , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
BAL preparations from undepleted mice contained numerous IL 2 , IL 4 and IFN gamma producing cells , with many fewer secreting TNF or IL 10 . ^^^ In addition to IL 2 , IL 4 , and IFN gamma , large numbers of cells were found to contain IL 10 and TNF beta transcripts . ^^^ Somewhat surprisingly , mRNA for IFN gamma , IL 5 , and TNF beta was prevalent in cells that had engulfed latex particles , though mRNA for IL 2 and IL 4 was never detected in macrophages . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Blocking experiments with cultured thyrocytes from two Graves ' glands and MoAbs to tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) did not prevent the occurrence of ICAM 1 expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In agreement with previous studies using rodent derived astrocytes , we found that human astrocytes ( fetal ) , could not initiate a primary T cell response even after up regulation of MHC class 2 antigen expression with interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Compared with mice undergoing LPS induced lethality during GVHR , Th 2 protected mice had : 1 ) lower levels of donor CD8+ lymphoid engraftment , 2 ) in vivo suppression of IFN gamma mRNA , 3 ) in vivo augmentation of IL 4 mRNA , and 4 ) a reduction in serum TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although serum levels of IFN gamma and TNF were normally not detectable in LCMV infected mice , infection acted synergistically with IL 12 for cytokine induction . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1 beta and tumor necrosis factor alpha ( TNF alpha ) increased the EGF receptor surface expression only on BT 20 breast carcinoma cells . 12 O tetradecanoylphorbol 13 acetate ( TPA ) induced up regulation of EGF receptor on MDA MB 468 cells , and a marginal but significant increase was determined in BT 20 cells and in interferon gamma ( IFN gamma ) treated MDA MB 468 cells . ^^^ CD 15 ( Lewisx ) antigen was down regulated on MDA MB 468 cells by TNF alpha , TPA , IL 1 alpha , as well as by IFN gamma and all trans retinoic acid . 1 , 25 ( OH ) 2 vitamin D 3 up regulated the CD 15 antigen surface expression on MDA MB 468 cells . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of the inflammatory cytokines , tumor necrosis factor ( TNF alpha ) , interleukin 1 alpha ( IL 1 ) , and gamma interferon ( IFN gamma ) on macro and microvessel derived endothelial cell proteolytic , adhesion protein and prostaglandin synthetic activities were compared . ^^^ All of the aforementioned effects of TNF alpha on HUVE uPA dependent activities were abrogated by co or pretreatment with IFN gamma . ^^^ In contrast , endothelium derived from human lung ( HLE ) exhibited high constitutive uPA and uPA dependent matrix degradation and rapid tube formation in Matrigel , activities all unaffected by TNF alpha or IFN gamma . ^^^ Co incubation with IFN gamma synergistically elevated TNF alpha or IL 1 induced ICAM 1 expression in HUVE , HLE , and HSE . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
T 2 has no effect on TNF alpha and IL 1 mediated ICAM 1 upregulation , showing its specificity for IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cell lysis and suppression of LNC can not be simulated by IFN gamma , by the combination of IFN gamma and TNF , or by BVI / 5 supernatants . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , treatment of target cells with TNF alpha or IFN gamma for 24 hr increased the expression of ICAM 1 in HT 29 , A 431 and MDA MB 468 ( ICAM 2 was induced on IGROV 1 ) and also enhanced the sensitivity of these target cells to biMAb targeted cytotoxicity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Activation of the human immature natural killer cell subset by IL 12 and its regulation by endogenous TNF alpha and IFN gamma secretion . ^^^ The IL 12 mediated activation of cytotoxicity was dependent on endogenous TNF alpha secretion and was suppressed by endogenous ( interferon gamma ( IFN gamma ) secretion . ^^^ However , IL 2 mediated activation was inhibited by anti TNF alpha antibody , but was not affected by endogenous IFN gamma secretion . ^^^ IL 12 also upregulated the expression of the 75 kDa TNF receptor ( TNF R p 75 ) and the upregulation was inhibited by anti IFN gamma antibody . ^^^ The 55 kDa TNF receptor ( TNF R p 55 ) , however , was not affected by IL 12 , but in the presence of anti IFN gamma antibody , its expression was enhanced . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Biological assays to measure IL 2 / IL 4 , IFN gamma and TNF alpha / TNF beta and Northern blot analysis to detect mRNA encoding bovine IL 2 , IL 4 , IFN gamma , TNF beta and TNF alpha revealed the differential production of cytokines by clones with different antigen specificities . ^^^ Two Bb 1 specific T cell clones produced the Th 1 pattern of cytokines : IL 2 , IFN gamma , TNF beta and TNF alpha , but not IL 4 . ^^^ Clones specific for the 42 kDa protein produced undetectable levels of all cytokines , but expressed an unrestricted or Th 0 pattern of cytokine mRNA : IL 2 , IL 4 , IFN gamma and TNF alpha . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When the cell lines were preincubated with compounds known to induce them to differentiate , production of tumor necrosis factor alpha ( TNF alpha ) and IL 1 beta was in most cases maximal in cells with differentiated characteristics , whereas NKSF / IL 12 production in response to LPS in all three producing cell lines was significantly enhanced only by pretreatment with dimethylsulfoxide ( DMSO ) for 24 hours , or by costimulation with interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We describe two patients with severe AD who were treated with oral cyclosporin A ( Cy A ) and in whom the serum levels of IL 1 alpha , IFN gamma and tumor necrosis factor alpha ( TNF alpha ) were sequentially measured . ^^^ Cy A , known to inhibit IFN gamma production in vitro , caused a rapid rise in serum IFN gamma , but not Il 1 alpha and TNF alpha , levels in the patients and the IFN gamma levels appeared to be inversely related to the therapeutic efficacy . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Within the 2 groups of IL 4 producing or non producing clones , further sub sets could be identified on the basis of differential production of IL 1 beta , IL 2 , IL 6 , IL 8 , IL 10 , TNF alpha , TNF beta and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Previous experiments revealed an influence of He Ne laser irradiation on concentrations of interleukin 1 alpha ( IL 1 alpha ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 2 ( IL 2 ) , and interferon gamma ( IFN gamma ) in supernatants of cultures of human peripheral blood mononuclear cells ( PBMC ) with increased cytokine concentrations after irradiation of 18 . 9 J / cm2 and decreased concentrations after irradiation of 37 . 8 J / cm2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of ICAM 1 was upregulated most consistently by treatment with interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) , cytokines that are released into the urine after intravesical BCG treatment . ^^^ Monoclonal antibodies to ICAM 1 and LFA 1 significantly decreased the binding of LAK cells to the cell lines that express ICAM 1 ( 37 to 75 % reduction , p < 0 . 05 ) , and cytokine treatment ( IFN gamma , TNF alpha ) of these cells enhanced ICAM 1 dependent adherence ( 18 to 39 % increase , p < 0 . 05 ) . ^^^ Cytokine treatment ( IFN gamma , TNF alpha ) of the tumor cells did not significantly increase LAK cell mediated cytolysis , despite upregulation of ICAM 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines produced by Th 1 clones such as IFN gamma and TNF alpha were indicated not to be involved in induction of the IL 2R alpha chain expression or proliferation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we examined the effect of three proinflammatory cytokines , tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 beta ( IL 1 beta ) , and interferon gamma ( IFN gamma ) , on the expression of ICAM 1 by primary rat astrocytes . ^^^ Astrocytes constitutively express ICAM 1 mRNA and protein , which is enhanced by treatment with TNF alpha , IL 1 beta and IFN gamma . ^^^ TNF alpha is the most potent inducer of ICAM 1 expression , followed by IL 1 beta , then IFN gamma . ^^^ Kinetic analysis demonstrated optimum ICAM 1 mRNA expression after a 1 h exposure to TNF alpha , 2 h exposure to IL 1 beta , and 4 h exposure to IFN gamma . ^^^ Peak ICAM 1 protein expression was detected 12 16 h after treatment with TNF alpha or IL 1 beta , and after a 24 h exposure to IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We examined the changes in intercellular adhesion molecule 1 ( ICAM 1 ) expression on brain endothelium in response to tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Further analysis revealed that interleukin 10 ( IL 10 ) inhibited , whereas tumor necrosis factor alpha ( TNF alpha ) and IL 12 enhanced , IFN gamma production by spleen cells from infected or uninfected mice . ^^^ The combination of IL 12 and TNF alpha induced higher levels of IFN gamma from whole spleen cells of infected mice than from those of uninfected mice . ^^^ Depletion of the adherent cell population from the spleen cells of infected mice led to a significant reduction in the levels of IFN gamma produced after stimulation with IL 12 plus TNF alpha . ^^^ These data indicate that other cytokines produced by the adherent cell population from infected mice may be involved in maximal production of IFN gamma by NK cells stimulated with IL 12 and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Upon priming , these cells develop into cells that produce either IFN gamma , TNF beta , and IL 2 or IL 4 and its congeners . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Compared with cytokine inducers , IL 12 ability to induce IFN gamma production was severalfold higher than its ability to induce GM CSF or TNF alpha . ^^^ Likewise , the synergistic effect of IL 12 with the other stimuli to induce IFN gamma was stronger than that observed in the case of GM CSF or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These data demonstrate that : ( 1 ) the constitutive density of MHC class 1 antigens determines the extent of TCL lysis ; ( 2 ) IFN gamma , and not IFN alpha or TNF alpha controls the amount of K target cell lysis by increasing their MHC class 1 antigen expression ; and ( 3 ) IFN gamma and IFN alpha control the amount of PBMC target cell lysis by a mechanism independent of MHC class 1 , ICAM 1 or LFA 1 expression . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The LAK sensitivity of SMKT R 3 was decreased by treatment with a combination of interferon gamma ( IFN gamma ) and tumor necrosis factor ( TNF ) . ^^^ When actinomycin D ( a protein synthesis inhibitor ) was added to the culture medium prior to treatment with IFN gamma and TNF , the LAK sensitivity of SMKT R 3 recovered to the level demonstrated by the cells that had not received any cytokine treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Analysis of the cytokine response to SEA in vivo showed that IL 2 and tumour necrosis factor ( TNF ) were mainly produced by CD4+ T cells , while interferon gamma ( IFN gamma ) was predominantly released by CD8+ T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increases in serum IFN gamma , TNF alpha , and nitrogen oxides were demonstrated , exceeding those observed with IL 2 treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cellular expression and shedding of ICAM 1 are up regulated by cytokines , such as tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) . ^^^ The measurement of serum IFN gamma and TNF alpha also revealed high concentrations of the cytokines in miliary tuberculosis and far advanced pulmonary tuberculosis . ^^^ The circulating ICAM 1 values were significantly correlated to serum IFN gamma and TNF alpha values . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
If mixtures of TNF and IL 1 or mixtures of TNF and IFN gamma are used to challenge mice previously primed with IFN gamma or IL 12 , mortality is induced . ^^^ When the mice are primed with LPS , the combination of TNF , IL 1 , and IFN gamma induced only a partial mortality incidence suggesting that the involvement of other LPS induced factors . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
ICAM 1 expression was enhanced by IL 1 beta , TNF alpha , and most effectively by IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It was observed that the antiproliferative effects of IFN gamma and TNF alpha on the cultures of SK BR 3 cells were associated with reduction in the steady state levels of P185HER2 with no change in the steady state levels of expression of c erbB 2 mRNA . ^^^ Treatment of SK BR 3 cells with either IFN gamma or TNF alpha was accompanied by inhibition of rate of synthesis of the protein , enhanced turnover of newly synthesized P185HER2 , and reduced expression of P185HER2 on the cell surface . ^^^ These observed effects on the expression of P185HER2 were more pronounced by more growth inhibitory TNF alpha than IFN gamma . ^^^ These observations suggest that the growth regulation of SK BR 3 cells by IFN gamma and TNF alpha may be associated with reduced expression P185HER2 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Two melanoma cell lines ( M 19 , M 26 ) , 1 B lymphoblastoid cell line ( Daudi ) , 1 erythroleukemia cell line ( K 562 ) , 1 Szary cell derived cell line ( SeAx ) , 1 cell line derived from a mycosis fungoides lesion ( MyLa ) and normal peripheral blood mononuclear cells ( PBMC ) were grown in the presence of interferon gamma ( IFN gamma ) , IFN alpha , interleukin 2 ( IL 2 ) , IL 6 and tumor necrosis factor alpha ( TNF alpha ) in various concentrations . ^^^ TNF alpha and IFN gamma enhanced the shedding in a time and dose dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pretreatment of mice with neutralizing anti IL 10 mAb before SEB challenge did not modify the release of TNF but led to increased and sustained IL 2 and IFN gamma serum levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treating human malignant melanoma cells with tumor necrosis factor alpha ( TNF alpha ) or interferon gamma ( IFN gamma ) causes a dose and time dependent increase in surface expression of ICAM 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
They also produced IL 3 and IL 8 but not IL 2 , IFN gamma , TNF alpha or TNF beta in response to autologous tumor cells . ^^^ CD4+ potential T cell clones , but not CD8+ clones , that were established from freshly isolated TILs without in vitro sensitization by autologous tumor cells also produced IL 4 , IL 6 and IL 10 but not IFN gamma or tumor necrosis factor ( TNF ) alpha in an autologous tumor specific fashion . ^^^ PMA and PHA stimulated these potential T cell clones , regardless of their specific lymphokine production , to produce IL 3 , IL 4 , IL 6 , IL 8 , IL 10 , GM CSF , TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1 beta and TNF alpha induced a parallel increase of both the ICAM 1 expression on EC and sICAM 1 production by EC , while IFN gamma induced only the dose dependent enhancement of ICAM 1 expression , but not the production of sICAM 1 , IL 4 and IL 6 did not show any effects on the ICAM 1 expression nor on the sICAM 1 production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , the residual ICAM 1 inducing activity ( up to 30 % of ICAM 1 induction ) could not be associated with any known ICAM 1 inducers ( IFN gamma , TNF alpha , TNF beta , IL 1 alpha , IL 1 beta , IL 4 , retinoic acid , LPS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Elevated plasma levels of tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) were detected at the MTD . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
An increasing body of data shows a dual role during malaria attack for some cytokines released by monocytes and macrophages ( TNF , IL 1 , IL 6 ) or by T cells ( IFN gamma , lymphotoxin ( LT ) , IL 4 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These patients showed markedly increased serum interleukin 6 ( IL 6 ) levels after BMT , whereas other cytokines such as interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and IL 2 were not increased compared with pretreatment levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , these results do not necessarily mean that the increased levels of IL 6 , IFN gamma and TNF alpha induced acute GVHD ; they merely show that acute GVHD is observed more frequently in patients with increased IL 6 , IFN gamma and TNF alpha levels than in those with normal levels . ^^^ Although increased IL 6 levels were also observed in patients without acute GVHD , concomitant increase of IFN gamma and TNF alpha was not detected in such cases , showing that IL 6 can be increased by even graft versus host reaction ( GVHR ) which may not develop into clinical acute GVHD . ^^^ Taken together , acute GVHD appeared to be induced by synergistic interaction of IL 6 , IFN gamma and TNF alpha , consistent with a cytokine cascade . ^^^ Although IFN gamma levels were slightly increased in chronic GVHD and sometimes aggravated the disease status , IL 6 and TNF alpha appeared to be more closely involved in the induction of chronic GVHD . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
During the acute phase of both primary and secondary infections , cytokines commonly associated with innate resistance , TNF alpha , IFN gamma , IL 1 beta and IL 6 , were expressed . ^^^ During reinfection , mRNAs for TNF alpha and IL 6 were 10 fold and for IFN gamma about 50 fold higher than during primary challenge . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
For this purpose , we analyzed the levels of IL 1 beta , IL 2 , IL 4 , IL 5 , IL 6 , IL 8 , GM CSF , TNF alpha , and IFN gamma , as well as soluble IL 2 and TNF receptors , in concentrated bronchoalveolar lavage ( BAL ) fluid obtained from clearly defined patients with allergic and nonallergic asthma , eosinophilic pneumonia , allergic bronchopulmonary aspergillosis ( ABPA ) , hypersensitivity pneumonitis , and idiopathic pulmonary fibrosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum cytokines ( IL 6 , TNF alpha , IL 1 beta and IFN gamma ) in ankylosing spondylitis : a close correlation between serum IL 6 and disease activity and severity . ^^^ The aim of our study was to analyse the serum interleukin 6 ( IL 6 ) , tumour necrosis factor alpha ( TNF alpha ) , interleukin 1 beta ( IL 1 beta ) and interferon gamma ( IFN gamma ) levels in patients with AS and their relationship with disease activity . ^^^ An ELISA test was used to analyse serum cytokine ( IL 6 , TNF alpha , IL 1 beta and IFN gamma ) levels in 69 patients with AS . ^^^ IL 6 and TNF alpha serum levels , but not IL 1 beta and IFN gamma , were significantly higher in AS than in NIBP . ^^^ However , patients with RA showed higher serum levels of IL 6 , TNF alpha and IFN gamma than both AS and NIBP patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The binding to these cell lines was trypsin sensitive , in part required divalent cations , was blocked by an anti CD 6 mAb , and could be downregulated by tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 beta ( IL 1 beta ) and interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokines that we used were recombinant GM CSF , natural type TNF alpha , recombinant interferon gamma ( IFN gamma ) , and recombinant IL 2 . ^^^ On the other hand , significant growth inhibition of MAC was observed in the macrophages treated with GM CSF or TNF alpha , while no growth inhibition of MAC was observed in the macrophages treated with IFN gamma or IL 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Administration of sTNFR : Fc also diminished the increase in IL 1 beta , IL 6 , TNF alpha and interferon gamma ( IFN gamma ) mRNA normally found in the lungs following haemorrhage . ^^^ In contrast to the effects of sTNFR : Fc on cytokine mRNA levels among intraparenchymal pulmonary mononuclear cells , such therapy following haemorrhage was associated with increased amounts of mRNA for TNF alpha among peripheral blood mononuclear cells , as well as increased IFN gamma titres in serum and bronchoalveolar lavage ( BAL ) specimens . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The present study was designed to establish ( 1 ) the effects of cytokines on soluble ICAM 1 ( sICAM 1 ) production by human synovial cells ( SC ) and ICAM 1 expression on these cells , and ( 2 ) the effects of sICAM 1 on lymphocyte SC adhesion . sICAM 1 production was enhanced in parallel with ICAM 1 expression by IL 1 beta , TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
One group exemplified by psoriasis is characterized by consistently detectable mRNAs for IL 2 , IFN gamma , and TNF alpha , but not IL 4 , IL 5 , IL 10 , thereby closely resembling the murine Th 1 type cell mediated response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vivo and in vitro production of tumor cytotoxic cytokines tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) was significantly stimulated following IL 2 therapy , further indicating IL 2 mediated stimulation of the antineoplastic activity of the immune system . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1 beta , IFN gamma , and TNF alpha mRNA became detectable in spleen cells using the PCR technique . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Previous studies which demonstrated that cytokines such as interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) are essential for the development of graft versus host reaction ( GvHR ) did not establish whether the individual cytokines were responsible for distinct features of the disease . ^^^ In this report , we show that IFN gamma production is associated with the early proliferative phase of the disease , whereas the late , destructive phase correlates with production of TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Reverse transcription polymerase chain reaction analysis was used to determine tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 beta ( IL 1 beta ) , IL 6 , IL 10 , interferon gamma ( IFN gamma ) , monocyte chemotactic protein 1 ( MCP 1 ) , macrophage inflammatory protein 2 ( MIP 2 ) , and IL 1 receptor antagonist ( IL 1RA ) mRNA expression . ^^^ Elevated expression of TNF alpha , IL 1 beta , IL 6 , IFN gamma , MCP 1 , and MIP 2 was also observed concomitant with maximal uveitis , at 22 to 24 hours . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Blood monocytes upregulated induction of cytotoxic CD8+ cells stimulated with NKSF alone , and this effect was abolished by addition of antibody against IFN gamma , but not of antibody against tumor necrosis factor alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The presence of various cytokines , namely the tumor necrosis factor ( TNF alpha ) , interferon ( IFN gamma ) , and interleukins ( IL 1 beta and IL 6 ) , was investigated in seminal plasma of fertile , infertile , and immunoinfertile men using specific immunoradiometric assays . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Effects of palmitic , stearic , oleic , and linoleic acid on mitogen induced DNA synthesis , on production of IL 1 beta , IL 2 , IFN gamma , and TNF alpha , and on IL 2R expression were determined in human peripheral lymphocytes . ^^^ Palmitic acid enhanced the release of IFN gamma at concentrations that diminished TNF alpha production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pooled mouse sera were analyzed by ELISA at various times after immunization for IL 1 alpha , IL 2 , IL 3 , IL 4 , IL 5 , IL 6 , IL 10 , IFN gamma , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We report here that TNF , IL 2 , and IFN gamma are the principal cytokines which were detected within hours of SEB administration , and that other cytokines such as IL 3 , IL 4 , IL 5 , IL 6 , IL 10 , GM CSF and M CSF were undetectable . ^^^ The TNF and IFN gamma staining patterns observed in lymph node sections were indicative of Golgi localized cytokine . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunohistochemical analysis revealed peak staining for interferon gamma ( IFN gamma ) and tumor necrosis factor ( TNF ) in lymph nodes of both strains on day 7 of infection , with elevated levels of IFN gamma and TNF persisting in CBA / Ca mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma treated ECs bound SEB effectively and stimulated T cells to proliferate and secrete tumor necrosis factor alpha ( TNF alpha ) and IFN gamma . ^^^ These antibodies also blocked production of the proinflammatory mediators , TNF alpha and IFN gamma , in SEB stimulated T cell EC cocultures . ^^^ Reducing proinflammatory mediators such as TNF alpha and IFN gamma by these antibodies in SEB induced T cell responses may be useful therapeutic strategy for circumventing SEB toxicity and pathogenesis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It is well known that bacterial lipopolysaccharide ( LPS ) induces the synthesis of tumor necrosis factor alpha ( TNF alpha ) and other inflammatory cytokines by primary monocytes and macrophages and that the Th 1 lymphokines , interleukin 2 ( IL 2 ) and interferon gamma ( IFN gamma ) , augment this response . ^^^ We investigated the ability of IL 2 and IFN gamma to induce the production of TNF alpha mRNA and protein independently of LPS and the modulation of this response by macrophage colony stimulating factor ( M CSF ) and IL 10 . ^^^ We found that IL 2 and IFN gamma were both able to induce the accumulation of TNF alpha mRNA , albeit with slower kinetics than LPS , and that they acted synergistically . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We evaluated the effect of increasing doses of gamma irradiation on the release of interleukin 4 ( IL 4 ) and interferon gamma ( IFN gamma ) from lymphocytes , and interleukin 1 beta ( IL 1 beta ) and tumor necrosis factor alpha ( TNF alpha ) from monocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Transforming growth factor beta ( TGF beta ) and to a lesser extent interferon gamma ( IFN gamma ) were able to decrease GM CSF production induced by IL 1 and / or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interestingly , growth of both cell lines was stimulated by natural IL 1 and not affected by TNF alpha and IFN gamma , whereas growth of parental KY 821 was inhibited by these factors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We demonstrated with high reproducibility the expressions of messenger RNAs of cytokines , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) in the lung tissue of M . pulmonis infected mice by the reverse transcriptase polymerase chain reaction and confirmed specific mRNA of the cytokines by restriction endonuclease digestion . ^^^ The early lesions are characterized primarily by neutrophils and edema in the alveolar spaces . mRNAs prepared from the lung tissue of M . pulmonis infected and uninfected mice were also tested for the presence of messages specific to TNF alpha and IFN gamma by the reverse transcriptase polymerase chain reaction . ^^^ The expression of the genes encoding TNF alpha and IFN gamma was constitutively demonstrated from 24 hr through 35 days after the intranasal inoculation of M . pulmonis . ^^^ Furthermore , cells of two types , adherent and nonadherent cells , in bronchoalveolar lavage fluids obtained from the mice 3 weeks after inoculation of M . pulmonis were also found to express the genes of TNF alpha and IFN gamma respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This anti Candida activity of neutrophils was augmented by lipopolysaccharide from Escherichia coli , murine tumor necrosis factor ( TNF ) , murine interferon gamma ( IFN gamma ) and murine granulocyte macrophage colony stimulating factor ( GM CSF ) but not by granulocyte colony stimulating factor ( G CSF ) added to the incubation medium . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In fact , the IFN gamma and IL 1 induced adhesion of PBMC to cytokine treated HUVEC could be down regulated by the PKC inhibitor , whereas TNF alpha and LPS mediated adhesion was not affected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Five animals in each isograft and allograft group were sacrificed on POD 3 , 5 , 7 , 8 , 9 , 10 , 12 , and 14 . mRNA was isolated from portions of the terminal ileum and rtPCR performed to amplify message for interleukin 2 ( IL 2 ) , IL 2 receptor ( IL 2R ) , interleukin 6 ( IL 6 ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) . ^^^ On POD 5 the first evidence of mild rejection was seen on histology and IL 6 , IFN gamma , TNF alpha showed a significant up regulation in the allograft that persisted through POD 14 . mRNA for IL 2 was not significantly upregulated until POD 7 and persisted until POD 14 . ^^^ Histologic rejection was moderately severe by POD 7 and severe between POD 8 and 14 correlating with the increasing expression of IL 6 , IFN gamma , and TNF alpha . ^^^ In summary , we have shown that increasing expression of mRNA for IL 6 , IFN gamma , and TNF alpha not only correlated with severity of rejection but that upregulation began early when histologic evidence of rejection first occurred . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The early and progressive expression of TNF alpha and IL 1 beta as well as IFN gamma , intercellular adhesion molecule 1 , and MHC class 2 in IT rejection may contribute to the difficulty in controlling IT rejection with present immunosuppression . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha did not cause secretion of MCP 1 , but caused about the same amount of NAP 1 secretion as IL 2 + IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of HLE with a variety of cytokines and LPS up regulated both IL 8 mRNA expression and release of immunoreactive IL 8 with an order of potency tumor necrosis factor alpha ( TNF alpha ) > > IL 1 alpha > LPS , whereas interferon gamma ( IFN gamma ) had no effect on IL 8 mRNA or antigenic levels . ^^^ MCP 1 gene expression and secretion was induced in a dose dependent manner after IL 1 alpha , TNF alpha , IFN gamma , and LPS activation of HLE . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both the normal and systemic sclerosis dermal fibroblasts increased their cell surface expression of ICAM 1 in response to interleukin 1 beta ( IL 1 beta ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) in a dose dependent fashion . ^^^ Systemic sclerosis dermal fibroblasts appeared to be hyperresponsive to IL 1 beta , TNF alpha , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In other experiments , DC were pre incubated with selected proinflammatory cytokines ( IL 1 , IL 2 , IFN gamma , TNF alpha ) in order to determine their abilities to modulate cell adherence to ECM . ^^^ Pretreating DC with IFN gamma or TNF alpha enhanced DC binding to FN but did not increase binding to LN or COLL . ^^^ IFN gamma and TNF alpha increase adherence of DC to FN and may promote their accumulation in the lung during inflammation . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of interferon gamma ( IFN gamma ) , alone and in combination with IL 1 , IL 6 and tumour necrosis factor alpha ( TNF alpha ) , on in vitro erythropoietin ( Epo ) production by the human hepatoma Hep3B cell line were evaluated . ^^^ IFN gamma and IL 1 had an additive inhibitory effect , whereas IFN gamma and TNF alpha acted in a synergistic fashion in inhibiting Epo production by Hep3B cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Circulating CD2+ T cells obtained from patients with active HLH spontaneously secreted M CSF and IFN gamma in vitro , whereas circulating monocytes did not produce detectable levels of both M CSF and IFN gamma , but produced high levels of IL 6 and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha and IFN gamma potentiate the deleterious effects of IL 1 beta on mouse pancreatic islets mainly via generation of nitric oxide . ^^^ In order to further characterize the mechanism ( s ) of action of cytokines on insulin producing cells , mouse pancreatic islets were exposed for 48 h to IL 1 beta , IFN gamma or TNF alpha , alone or in combinations . ^^^ Both IL 1 beta and TNF alpha , but not IFN gamma , induced NO production and expression of the mRNA encoding for the inducible form of the enzyme NO synthase ( iNOS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Peritoneal macrophages from Toxoplasma infected mice ( 10 cysts of Beverley strain / mouse ) were harvested 8 weeks after infection , and incubated with the mitogen induced lymphokine , recombinant mouse interferon gamma ( IFN gamma ) , recombinant mouse tumor necrosis factor alpha ( TNF alpha ) alone or in combination with IFN gamma ( IFN gamma / TNF alpha ) for 24 hr at 37 degrees C , 5 % CO 2 . ^^^ IFN gamma or IFN gamma / TNF alpha treated macrophages from Toxoplasma infected mice revealed significantly higher H2O2 production than resident macrophages from Toxoplasma infected mice . ^^^ The production of NO 2 by TNF alpha , IFN gamma or IFN gamma / TNF alpha treated macrophages from Toxoplasma infected mice were significantly higher than that by resident macrophages , whereas lymphokine treated group produced similar amount as that produced by resident macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) and interleukin 1 beta ( IL 1 beta ) were detected at markedly high levels at 24 h , and interferon gamma ( IFN gamma ) was detected at 120 h . ^^^ Neutralizing anti TNF alpha monoclonal antibody significantly reduced the anti proliferative activity of ACHN cells , and anti IFN gamma antibody reduced that of T 24 cells . ^^^ Tumor necrosis factor alpha ( TNF alpha ) and interleukin 1 beta ( IL 1 beta ) were detected at markedly high levels at 24 h , and interferon gamma ( IFN gamma ) was detected at 120 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin 1 alpha ( IL 1 alpha ) , interferon gamma ( IFN gamma ) , interleukin 8 ( IL 8 ) , and tumour necrosis factor alpha ( TNF alpha ) were tested for their chemotactic effectiveness in a modified Boyden chamber assay . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Unlike in Graves ' retrocular fibroblasts , hydrogen peroxide was not an effective stress in FRTL 5 , and the induction was not suppressed by methylmercaptoimmidazole and propylthiouracil , nor enhanced by interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increased glomerular interferon gamma ( IFN gamma ) was detected within 24 h of induction of NCGN , and IL 1 beta and tumour necrosis factor alpha ( TNF alpha ) were found from day 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Levels of FN in the supernatant of cultured human RPE cells were quantified with an ELISA , after which the cells were stimulated with human recombinant cytokines , interleukin 1 beta ( IL 1 beta ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 6 ( IL 6 ) , interferon gamma ( IFN gamma ) , transforming growth factor beta 1 and 2 ( TGF beta ) , and phorbol myristate acetate ( PMA ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To determine the regulatory properties of ICAM 1 , Ia , and MHC class 1 ( H 2K ) molecules in LC , we have examined the effects of interleukin ( IL ) 1 alpha , IL 1 beta , IL 2 , IL 3 , IL 4 , IL 5 , IL 6 , IL 7 , IL 10 , interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and granulocyte macrophage colony stimulating factor ( GM CSF ) on the expression of these molecules . ^^^ IL 10 potently inhibits the TNF alpha induced ICAM 1 up regulation , but not the IFN gamma induced H 2K up regulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Synergistic activation of intercellular adhesion molecule 1 ( ICAM 1 ) by TNF alpha and IFN gamma is mediated by p65 / p50 and p65 / c Rel and interferon responsive factor Stat 1 alpha ( p 91 ) that can be activated by both IFN gamma and IFN alpha . ^^^ Human ICAM 1 expression is up regulated by IFN gamma and TNF alpha and synergistically increased by a combination of both . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF secretion , modulated by interferon gamma ( IFN gamma ) and interleukin 10 ( IL 10 ) , is followed by release of other cytokines such as interleukins ( IL ) ( IL 1 , IL 6 and IL 8 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Low doses of TNF alpha inhibited the up regulation of Ly 6C induced by IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) is a product of activated T lymphocytes , and tumor necrosis factor alpha ( TNF alpha ) is a product of both lymphocytes and macrophages . ^^^ The purpose of this study was to characterize the effects of TNF alpha and IFN gamma on a human submandibular gland epithelial cell line ( HSG ) . ^^^ When these cytokines were added in combination ( 20 units / ml TNF alpha and 1 , 000 units / ml of IFN gamma ) , there was a synergistic antiproliferative effect ; no apparent cell growth was observed . ^^^ The antiproliferative effect of IFN gamma alone and that of IFN gamma and TNF alpha in combination were blocked completely using an antibody to the IFN gamma receptor . ^^^ Treatment of HSG cells with IFN gamma , with or without TNF alpha , resulted in increased levels of ICAM 1 and the acquisition of HLA DR expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The use of another macrophage activator , interferon gamma ( IFN gamma ) , yielded essentially similar results . +D and D mice were injected with LPS and TNF alpha levels in the serum were measured . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
ICAM 1 and VCAM 1 were inducible in a dose dependent fashion in the endothelial cells by interleukin 1 alpha ( IL 1 alpha ) , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) . ^^^ Expression of HLA DR in endothelial cells was inducible by IL 1 alpha and IFN gamma and not by TNF alpha . ^^^ Expression of E selectin on endothelial cells was induced only by IL 1 alpha , not by IFN gamma or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Constitutive production of the cytokine TNF , or its liberation in response to either interferon gamma ( IFN gamma ) or lipopolysaccharide ( LPS ) alone , was very low in RMG and RPE cells , irrespective of the strain . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Reverse transcriptase and the polymerase chain reaction ( PCR ) were used to amplify mRNA for interleukins 1 alpha ( IL 1 alpha ) , 2 ( IL 2 ) , 4 ( IL 4 ) , IL 2 receptor alpha ( IL 2R alpha ) , tumour necrosis factor alpha ( TNF alpha ) and gamma interferon ( IFN gamma ) with beta actin as an internal control . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Characterization of astrocyte nuclear proteins involved in IFN gamma and TNF alpha mediated class 2 MHC gene expression . ^^^ TNF alpha alone has no effect on class 2 MHC expression , but enhances IFN gamma induced class 2 expression . ^^^ IFN gamma acts by inducing transcription of the class 2 gene , and TNF alpha enhances the rate of IFN gamma induced transcription . ^^^ We have previously described two factors , IFN gamma enhanced factor 10 ( IFNEX ) and TNF alpha induced complex 10 ( TIC 10 ) , whose expression is induced by IFN gamma and IFN gamma / TNF alpha , respectively , which interacted with the 10 box of the DRA promoter . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interactions with IFN gamma and TNF alpha . ^^^ Toxoplasma gondii is a major pathogen of immunocompromised hosts , and one defense mechanism against the parasite is activation of macrophages ( M phi ) for toxoplasmacidal activity by IFN gamma after triggering by TNF alpha . ^^^ IL 6 , IFN gamma , and TNF alpha are cytokines involved in inflammatory responses and are induced by T . gondii infection . ^^^ Combining TNF alpha with IL 6 and IFN gamma pretreatment resulted in restoration of toxoplasmacidal activity . ^^^ Addition of a polyclonal anti TNF alpha Ab to IL 6 and IFN gamma pretreatment resulted in enhancement in the IL 6 mediated impairment of IFN gamma function . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Evidence of transient tumor necrosis factor ( TNF ) secretion is detected in serum within 1 h of SEB administration , and massive amounts of interferon gamma ( IFN gamma ) and interleukin 6 ( IL 6 ) are present within 4 6 h . ^^^ Mice are partly protected by treatment with dimeric soluble TNF receptor Fc fusion protein or the nitric oxide synthase inhibitor , aminoguanidine , neither of which blocks SEB induced IFN gamma or IL 6 production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both TNF alpha and interferon gamma ( IFN gamma ) limited mycobacterial replication . ^^^ TGF beta 1 ( 10 ng / mL ) abrogated the bacteriostatic effects of TNF alpha and IFN gamma . ^^^ Within the infected focus , TGF beta 1 produced by mononuclear phagocytes may play an important role in the pathogenesis of tuberculosis , in part by modulating the response to potentially protective cytokines such as TNF alpha and IFN gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
No significant IL 6 production by PTEC could be induced by TNF alpha , IL 2 , IFN gamma , or LPS over a broad dosage range . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition there was a close correlation between the value of IFN gamma and of TNF alpha produced by PBMC in the patients on haemodialysis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To investigate the mechanisms controlling ICAM 1 gene expression , we have identified regulatory DNA sequences responsible for maintaining basal and mediating induced transcription in response to tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) . ^^^ Regulatory elements dedicated to TNF alpha and IFN gamma were identified 190 and 90 bp , respectively , upstream from the ICAM 1 transcription start site . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Simultaneous administration of TNF alpha ( 10 1000 units ) , however , abrogated the mitogenic effects of either CD 8 SN or IFN gamma ; the inhibitory effect of TNF alpha was lost if applied 2 days following IFN gamma treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Compared with KOS vaccinated mice , the gE vaccinated mice had a similar pattern of IFN gamma , but a delay in the appearance of CD4+ T cells , CD8+ T cells , and IL 4 , IL 6 , and TNF alpha containing cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Intra articular injection of the cytokines IL 1 alpha and TNF alpha , but not IFN gamma , induced marked PMNL accumulation in the joints ; this was strongly inhibited by the treatment of rats with anti LFA 1 and anti Mac 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
BACKGROUND : Interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF ) synergize in inducing human neuroblastoma cells to differentiate terminally in vitro into mature nonproliferating neurons . ^^^ PURPOSE : To understand the basis of IFN gamma TNF synergism , we investigated the constitutive equipment of receptors to IFN gamma and TNF in two human neuroblastoma cell lines ( i . e . , LAN 5 and GI LI N ) and their quantitative and functional variations following treatment with IFN gamma or TNF . ^^^ METHODS : IFN gamma receptors and TNF receptors were assessed and functionally characterized by radioreceptor binding assay before and after treatment of the cells with IFN gamma or TNF . ^^^ RESULTS : No quantitative or functional modification of IFN gamma receptors was observed in TNF treated cells . ^^^ However , after treatment with IFN gamma , TNF receptor numbers were enhanced to a different extent in both cell lines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Apart from TNF , other exogenously applied recombinant cytokines ( IL 1 , IL 6 , IFN gamma , GM CSF ) were not cytotoxic to U 937 DMSO cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study we have examined the potential role of xanthine oxidase ( XO ) in the intracellular oxidative stress induced by combinations of recombinant murine TNF alpha ( rMuTNF alpha ) and murine interferon gamma ( IFN gamma ) in cultured mouse hepatocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We conclude that subjection of a lung allograft to standard periods of cold warm ischemia will result in a temporary early elevation of IL 2 , TNF alpha , and IFN gamma detectable only in the bronchoalveolar compartment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
GVEC were stimulated with IFN gamma or TNF alpha in the presence or absence of GN , and tested for changes in levels of MHC class 1 , MHC class 2 , vascular cell adhesion molecule 1 , and intercellular adhesion molecule 1 expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neutralizing antibodies against TNF alpha , TNF beta and IFN gamma ( which are also produced by OKT 3 activated MM T cells ) did not prevent it , and Transwell cultures showed that cell to cell contact was the main mechanism involved . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
All strains produced IL 6 , TNF alpha , IFN alpha / beta , and IFN gamma when spleen cells were cultured for 48 hr with loxoribine . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , when we incubated HUCB in IL 2 , IL 4 , IL 7 , IL 12 , TNF alpha , IFN alpha , IFN gamma , or TGF beta for 5 days , we observed that HUCB was capable of generating LAK cells only when incubated with either IL 2 or IL 12 . ^^^ In contrast , IL 2 , IL 7 , IL 12 , TNF alpha , and IFN gamma all generated LAK cells from adult PBL . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The secretory ( tumor necrosis factor , TNF alpha ; nitrite ) and cellular response ( mitochondrial respiration , TNF alpha independent tumoricidal activity ) of a pure , lymphocyte free population of resting , unprimed rat bone marrow derived mononuclear phagocytes ( BMM phi ) to direct interaction with viruses , protozoa , and fungi was assessed and compared with that triggered by bacterial agents and interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines , such as TNF , IL 1 , and IFN gamma are involved in the inflammatory demyelinating process by enhancing vascular permeability , modulating inflammatory cell cell interactions and injuring the blood brain barrier . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our results show that all T cells reactive with bacteria produce interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) , but no interleukin ( IL ) 4 and no or very little IL 2 and IL 10 and , thus , belong to the Th 1 subset , while T cells reactive with tetanus toxoid or Anisakis belong to the Th 0 subset with production of IFN gamma , IL 2 , IL 4 , IL 10 and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Liposomal LPS also induced more active accumulation of IFN gamma in human peripheral blood mononuclear cells and less active accumulation of monokines , contributing to the realization of the toxic properties of endotoxin ( IL 1 alpha , TNF alpha , IL 6 and GM CSF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Intravesical growth of MB 49 results in the host derived expression of mRNA for both interleukin 4 ( IL 4 ) ( TH 2 ) and interferon gamma ( IFN gamma ) ( TH 1 ) , as well as tumor necrosis factor alpha ( TNF alpha ) expression of indeterminate origin . ^^^ Intravesical instillation of bacillus Calmette Gurin ( BCG ) , highly effective in eliminating bladder tumors clinically and in experimental systems , results in IFN gamma and TNF alpha mRNa production in the bladder wall , but no IL 4 . ^^^ Following BCG treatment of intravesical MB 49 , the number bladders expressing IL 4 mRNA decreases , while IFN gamma and TNF alpha expression remains constant . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytotoxic elements in ' crisis ' serum are uncertain but may include TNF , gamma interferon ( IFN gamma ) , and lipid peroxidases . ^^^ Killing of parasites by neutrophils is enhanced by immune serum and cytokines TNF alpha , IFN gamma and lymphotoxin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
On the other hand , FPA of P 2 and P 4 was suppressed by none of the antibodies against cytokines with FPA , such as FGF , IL 4 , IL 6 , IFN gamma , TGF beta and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha , IL 1 beta , and LPS caused a dose and time dependent increase of nitrite ( NO 2 ) , the stable metabolite of nitric oxide ( NO ) , in conditioned media over 48 hours , while IFN gamma had a minimal effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Growth and major histocompatibility antigen expression regulation by IL 4 , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) on human renal cell carcinoma . ^^^ IFN gamma caused a significant inhibition of RCC tumour cell growth ( up to 70 % ) in a dose dependent manner , whereas the effect of TNF alpha was more limited ( 0 20 % inhibition ) . ^^^ IL 4 and IFN gamma rendered RCC cells more responsive to the inhibitory effect mediated by TNF alpha . ^^^ The combination of TNF alpha with IL 4 and IFN gamma induced an optimal growth inhibition ( up to 90 98 % ) of RCC cells . ^^^ These observations confirm the direct interaction of IL 4 , IFN gamma and TNF alpha with RCC tumour cells , both at the level of growth regulation and MHC antigen expression , and suggest a therapeutic potential of the combination of the three cytokines for renal cell carcinoma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The induction of mRNA of interferon gamma ( IFN gamma ) or tumour necrosis factor alpha ( TNF alpha ) and the production of these cytokines in the non adherent LAK cells from LNC were also significantly reduced compared with those from PBMC or SPC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Previous work from this laboratory demonstrated that gamma interferon ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) are essential mediators of these processes . ^^^ In an attempt to elucidate early mechanisms of resistance , we investigated cytokine mRNA production , including that for interleukin 1 beta ( IL 1 beta ) , IL 2 , IL 4 , IL 6 , IL 10 , TNF alpha , and IFN gamma , after primary as well as secondary Y . enterocolitica infection in Yersinia susceptible BALB / c mice and Yersinia resistant C57BL / 6 mice . ^^^ In both strains of mice , proinflammatory cytokines such as IL 1 beta , IL 6 , and TNF alpha were expressed rapidly and to comparable degrees , while IFN gamma expression was enhanced two to eightfold in C57BL / 6 mice , as revealed by semiquantitative reverse transcription PCR . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha / interferon gamma ( TNF alpha / IFN gamma ) and interleukin 1 alpha ( IL 1 alpha ) / IFN gamma enhanced adhesion molecule expression and the adhesion of T cells to synovial cells . ^^^ Anti intercellular adhesion molecule 1 blocked adhesion of T cells to TNF alpha / IFN gamma and IL 1 alpha / IFN gamma stimulated synovial cells while an antibody to CD 61 blocked adhesion to IL 1 alpha / IFN gamma stimulated cells . ^^^ Tumor necrosis factor alpha / interferon gamma ( TNF alpha / IFN gamma ) and interleukin 1 alpha ( IL 1 alpha ) / IFN gamma enhanced adhesion molecule expression and the adhesion of T cells to synovial cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The activation by IFN gamma and / or infection with MHV 3 showed that BALB / c mouse macrophages were capable of releasing tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 ( IL 1 ) and anion superoxide ( O 2 ) , and A / J mouse macrophages were capable of releasing TNF alpha and IL 1 but not O 2 . ^^^ Comparable amounts of TNF alpha or IL 1 were released by IFN gamma activated A / J or BALB / c mouse macrophages . ^^^ Following MHV 3 infection or IFN gamma activation and MHV 3 infection , BALB / c mouse macrophages were always capable of releasing higher amounts of TNF alpha , IL 1 or O 2 than A / J mouse macrophages , which correlated with their susceptibility to the virus infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These analyses were compared to in situ expression of tumour necrosis factor alpha ( TNF alpha ) , interleukin 3 ( IL 3 ) , interferon gamma ( IFN gamma ) and IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Surprisingly , release of IL 6 and TNF alpha could be blocked completely by addition of anti IFN gamma . ^^^ This suggests that the release of IL 6 and TNF alpha in MLC is dependent on IFN gamma produced by T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Anti ICAM 1 also blocked the production of pro inflammatory mediators , TNF alpha and IFN gamma by SEB stimulated T cells . ^^^ Reducing toxic mediators such as TNF alpha and IFN gamma by anti ICAM antibodies in SEB induced T cell responses may be a useful therapeutic strategy to circumvent SEB toxicity and pathogenesis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Supernatants from HLA matched patient / donor mixed lymphocyte cultures ( MLCs ) were analysed for levels of tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) . ^^^ The results suggest that measurement of both TNF alpha and IFN gamma in HLA matched MLC supernatants is of predictive value and that the skin explant model is a useful model for studying the aetiology of GVHD in humans . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Effects of pentoxifylline , methylprednisolone , anti TNF , and anti IFN gamma antibodies . ^^^ Because TNF and IFN gamma are supposed to be involved in the pathophysiology of the anti CD 3 response , we investigated the influence of in vivo neutralization of TNF and IFN gamma . ^^^ Injection of mAb to TNF and IFN gamma significantly reduced systemic TNF levels and both soluble TNFR levels . ^^^ Although TNF and IFN gamma are involved in this mechanism , their role does not seem to be crucial . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of this receptor in HT 29 . 74 human colon carcinoma cells is up regulated by the recombinant cytokines IFN gamma , TNF alpha , and IL 4 . ^^^ Analysis of four separate LPMC supernatants revealed mean concentrations of 8260 pg / ml for IFN gamma , 420 pg / ml for TNF alpha , and 15 pg / ml for IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma inhibits internalization of soluble aminated beta 1 , 3 D glucan by macrophages and thereby down regulates the glucan induced release of TNF alpha and IL 1 beta . ^^^ The stimulatory effect of AG on IL 1 beta and TNF alpha release from macrophages was reduced by pretreatment of the cells with IFN gamma . ^^^ The reduced internalization of AG after IFN gamma treatment of macrophages , is probably responsible for the down regulation of IL 1 and TNF alpha secretion . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The massive release of TNF alpha , IL 1 beta , IL 6 , IFN gamma , and 6kPGF1 alpha between 15 and 60 minutes after reperfusion was the specific result of xenoperfusion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumour necrosis factor alpha ( TNF alpha ) and gamma interferon ( IFN gamma ) are considered to be critical in the mechanism . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of cytokine genes , TNF alpha , TNF beta and IFN gamma , in human astroglial cell lines and in fresh brain specimens was studied by PCR . mRNA transcripts of TNF alpha could be detected in three out of five astrocytomas and neuroblastoma cell lines , and after stimulation with IL 1 beta / IFN gamma or LPS / IFN gamma all these cell lines expressed TNF alpha genes . ^^^ We were able to detect expression of IFN gamma genes within two astrocytoma cell lines , which interestingly did not show TNF alpha activity . ^^^ The concentration of these cytokines in the supernatant of cultured cells was measured quantitatively by TNF alpha , beta or IFN gamma ELISA . ^^^ The combined stimulation of these neuroglial cell lines with IL 1 beta and LPS or IFN gamma , revealed a high level of TNF alpha activity . ^^^ The concentration of TNF alpha in the supernatant of the IMR 32 neuroblastoma cell line increased markedly upon stimulation with IL 1 beta in both a time and dose dependent fashion in the presence of LPS or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A significant increase in interleukin 2 ( IL 2 ) and gamma interferon ( IFN gamma ) secretion was found in AD patients in the moderately severe stage of the disease , whereas in the mild stage of the disease there was a significant decrease in interleukin 3 activity ( IL 3 ) and tumor necrosis factor ( TNF ) levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Isolated perfusion of the limb with high dose tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) and melphalan for melanoma stage 3 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A phase 3 trial compares the three drug combination of TNF , interferon gamma ( IFN gamma ) and melphalan with a standard melphalan alone perfusion in a prospective randomized trial . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of in transit metastases from cutaneous melanoma by isolation perfusion with tumour necrosis factor alpha ( TNF alpha ) , melphalan and interferon gamma ( IFN gamma ) . ^^^ From December 1991 to July 1993 , 22 consecutive patients with grade IIIA IIIAB melanoma underwent isolation perfusion with TNF alpha ( 0 . 5 4 mg ) , melphalan ( 10 mg / l perfused limb ) and , in the first 12 cases , IFN gamma ( 1 . 5 10 10 ( 6 ) U ) . ^^^ The first series of 12 patients received a total dosage TNF alpha of 2 4 mg , and the second series of 10 cases received an escalating dosage of TNF alpha ( 1 . 5 1 . 0 0 . 5 mg ) and no IFN gamma before or during surgery . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytolysis of adenovirus infected murine fibroblasts by IFN gamma primed macrophages is TNF and contact dependent . ^^^ Adenovirus infected cells were lysed by IFN gamma primed RAW macrophages via a TNF and contact dependent mechanism under conditions in which little or no soluble TNF was detected in the supernatant of these effectors . ^^^ TNF involvement in the lytic mechanism of IFN gamma primed macrophages is shown by ( a ) cytolysis of TNF sensitive LM and adenovirus E1A expressing cells , ( b ) protection from cytolysis by the adenovirus E 3 14 . 7K protein and the E 3 10 . 4 / 14 . 5K complex of proteins , and ( c ) inhibition of cytolysis when neutralizing anti TNF serum is added to cocultures of macrophages and susceptible adenovirus infected targets . ^^^ These findings indicate that IFN gamma primed macrophages are cytolytic for TNF sensitive targets without soluble TNF release , but they lack the full cytolytic capacity of LPS triggered macrophages . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Prolonged treatment with TNF impaired production of IL 2 , IL 10 , IFN gamma , TNF , and lymphotoxin ( LT ) following stimulation with immobilized OKT 3 , and resulted in suboptimal expression of the IL 2R alpha chain ( Tac ) but not CD 3 , CD 4 , or HLA DR antigens , when compared to untreated control cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have shown that both rat and human hepatocytes maximally express the enzyme inducible nitric oxide synthase ( iNOS ) in response to a combination of LPS and the cytokines tumor necrosis factor ( TNF ) , interferon gamma ( IFN gamma ) , and interleukin 1 . ^^^ In this study , we show that the phenotypic change associated with the acute phase response following tissue injury primes the hepatocyte to subsequently express iNOS in vitro in response to LPS alone as well as TNF and IFN gamma . ^^^ Early postinjury changes result in maximal expression following stimulation with TNF or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Studies with stimulation of endogenous cytokines by E . coli LPS demonstrated induction of all of them within 3 hr in the blood stream with TNF alpha demonstrating peak at 1 hr , IL 1 alpha and IL 6 at 4 hr and IFN gamma between 6 9 hr with moderately high levels at 4 hr . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
For this study , the effects of murine recombinant interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) on LDV replication were examined . ^^^ Neither IFN gamma nor TNF alpha had any direct in vitro effect on LDV replication in cultured macrophages , suggesting that the actions of these cytokines required secondary or accessory in vivo events . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study aimed to investigate whether the biological response modifiers ( BRM ) interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) could enhance the cytotoxic action of cisplatin on ovarian tumour cells in vitro . ^^^ However , in one cell line at least ( PEO 1 ) , both TNF alpha and IFN gamma demonstrated a clearly synergistic effect with cisplatin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Addition of interferon gamma ( IFN gamma ) , but not bacterial lipopolysaccharide or mycobacterial lipoarabinomannan , as a second signal enhanced the TNF alpha and nitrite responses of macrophages plated onto fibrinogen , particularly in the Lshr macrophages . ^^^ Interaction with fibrinogen and fibronectin also primed macrophages for an enhanced TNF alpha response to leishmanial parasites , but this was only translated into enhanced nitrite responses in the presence of IFN gamma . ^^^ Hence , the specificity for the enhanced TNF alpha responses of Lshr macrophages lay in their interaction with fibrinogen and fibronectin ECM , while a differential nitrite response was only observed with fibrinogen and / or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The presence of IL 2 , IFN gamma , and TNF alpha and the absence of IL 4 mRNA in samples of ITL indicate a pattern of cytokine production that most closely resembles that of the TH 1 helper T cell subset . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Dose dependent effects on mRNA expression of IL 2 , GM CSF , IFN gamma , TNF alpha , and IL 6 were assessed using Northern blotting and slot blotting techniques . ^^^ A single application of 30 , 000 U nIL 2 induced selective and long lasting expression of IL 2 , IFN gamma , and GM CSF genes , which was not accompanied by accumulation of TNF alpha and IL 6 mRNAs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor bearing host peritoneal Mphi produced more NO and TNF alpha than normal host Mphi when activated with IFN gamma or LPS , respectively . ^^^ The experimental evidence leading to this conclusion included : 1 ) The Meth KDE cells produced significant levels of these cytokines . 2 ) Recombinant forms of these cytokines suppressed NO and TNF alpha production . 3 ) Ab mediated absorption of these cytokines from tumor cell supernatants restored NO and TNF alpha production . 4 ) Anti IL 10 and anti TGF beta 1 Ab addition to IFN gamma stimulated Mphi restored NO production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The results indicate that TNF , IFN gamma , IL 6 , IL 1 , and dexamethasone induce XDH / XO activity in bovine renal epithelial cells ( MDBK ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cells were stimulated with recombinant interferon gamma ( IFN gamma ) or tumor necrosis factor alpha ( TNF alpha ) , followed by immunolabeling for uPA or uPAR and flow cytometry . ^^^ IFN gamma increased surface uPA 2 . 2 fold relative to unstimulated controls ( P < . 001 ) , whereas TNF alpha had no significant effect . ^^^ Likewise , maximal uPA binding capacity was increased 2 . 8 fold by IFN gamma ( P < . 02 ) , but was not affected by TNF alpha . ^^^ IFN gamma upregulated uPAR 2 . 1 fold relative to unstimulated controls ( P < . 001 ) , whereas TNF alpha had no effect . ^^^ In contrast to effects on surface protein , TNF alpha induced a substantial increase in uPAR mRNA , equaling the effect of IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunohistochemistry demonstrated that the lymphocytic infiltrate consisted of CD8+ , HLA DR+ T lymphocytes ( some of which also stained for CD 38 ) , a marked depletion of epidermal Langerhans cells ( 90 % ) , and strong cytoplasmic staining of keratinocytes for IL 6 ( 60 % ) , IL 1 beta ( 50 % ) , tumour necrosis factor alpha ( TNF alpha ) ( 45 % ) and to a lesser degree , interferon gamma ( IFN gamma ) ( 35 % ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This may be due to increased biosynthesis of the potent vasodilator nitric oxide ( NO ) induced by cytokines such as tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) , which are known to be generated during IL 2 therapy . ^^^ Pretreatment TNF alpha and IFN gamma plasma concentrations were not significantly different in cancer patients from those in controls . ^^^ During infusion of IL 2 ( 18 10 10 ( 6 ) international units m 2 per day for 5 days ) these parameters increased , reaching maximal concentrations at day 3 for IFN gamma and day 5 for TNF alpha , neopterin and NOx . ^^^ The maximal induced NOx correlated with maximal TNF alpha ( r = 0 . 60 , P < 0 . 04 ) , IFN gamma ( r = 0 . 63 , P < 0 . 02 ) and neopterin ( r = 0 . 66 , P < 0 . 01 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The gamma delta T cells expressed mRNA for IFN gamma , TNF alpha , TGF beta 1 , and IL 6 . ^^^ CD8+ cells expressing IFN gamma , TNF alpha , and IL 6 in combination suggest a cytotoxic effector function . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The current report confirms that anti TNF alpha does inhibit activation of interferon gamma ( IFN gamma ) primed Mphi by paraformaldehyde fixed activated T cells . ^^^ Previous studies have demonstrated a role for autocrine TNF alpha stimulation in LPS induction of effector function in recombinant IFN gamma primed Mphi . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cells producing IL 2 , IFN gamma and particularly TNF beta dominated this in vitro response . ^^^ Exogenously added IL 2 also caused a five to tenfold increase in the number of IL 2 and IFN gamma producers but no apparent change in the kinetics of intracellular lymphokine appearance . ( 2 ) The cytokines IL 1 alpha , IL 1 beta , IL 6 and TNF alpha were produced by monocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The role of cytokines in clearance was investigated by using an RNase protection assay for interleukin 1 alpha ( IL 1 alpha ) , IL 1 beta , IL 2 , IL 3 , IL 4 , IL 5 , IL 6 , tumor necrosis factor alpha ( TNF alpha ) , TNF beta , and gamma interferon ( IFN gamma ) . ^^^ In immunocompetent mice there was upregulation of RNA for IL 1 alpha , IL 1 beta , IL 6 , TNF alpha , and IFN gamma at the time of clearance . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , the cytolytic effects of TNF alpha in the presence of IFN gamma correlated with an increased susceptibility of AML cells to MMC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The increase in 55 kDa TNF R mRNA was maximal at 2 4 h of exposure of IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In order to examine the regulation of cytokine receptor gene expression an RNase protection assay ( RPA ) was developed that allows the simultaneous and semiquantitative measurement of mRNAs encoding for the IL 1 p 60 and p 80 , TNF p 55 and p 75 , IFN gamma , and IL 6 receptors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
All the virulent strains of Listeria monocytogenes induced the expression of mRNAs specific for interleukin 1 alpha ( IL 1 alpha ) , gamma interferon ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) in the spleen of mice , while an L . monocytogenes strain incapable of producing listeriolysin O and strains of Listeria innocua induced the expression of TNF alpha mRNA only . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here we analyzed the role of gamma interferon ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) in the innate and acquired responses to M . avium infections in mice . ^^^ CD ( 4+ ) depleted mice showed enhanced bacterial proliferation and at the same time showed a reduction in the level of expression of both IFN gamma and TNF alpha mRNAs in spleen cells . ^^^ In vivo depletion studies using specific monoclonal antibodies showed that both IFN gamma and TNF alpha are involved in an early protection possibly involving NK cells , and furthermore , IFN gamma is involved in the later T cell protective response to infection . ^^^ In vivo neutralization of IFN gamma during M . avium infection also blocked the priming for enhanced TNF alpha secretion triggered by endotoxin . ^^^ These data suggest that both cytokines act in an additive or synergistic fashion in the induction of bacteriostasis and that IFN gamma is also involved in priming TNF alpha secretion . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this report , we show that the IFN gamma dependent inhibition of IL 8 release by PMN stimulated with lipopolysaccharide ( LPS ) , tumor necrosis factor ( TNF ) , and / or interleukin 1 beta ( IL 1 beta ) , but not with Y IgG , is a transient phenomenon . ^^^ Unlike IL 8 , the LPS stimulated production of TNF and IL 1 beta , as well as the TNF stimulated production of IL 1 beta , was markedly enhanced by IFN gamma over the entire incubation period ( up to 18 hr ) . ^^^ Addition of anti TNF and anti IL 1 beta antibodies to IFN gamma plus LPS treated PMN indicated that the LPS induced production of endogenous TNF and IL 1 beta , which was further potentiated by IFN gamma pretreatment , mediated in autocrine fashion the enhanced LPS induced IL 8 accumulation observed at 18 hr . ^^^ Furthermore , as shown by Northern blot analysis , all the effects of IFN gamma on LPS stimulated PMN were paralleled by changes at the level of TNF , IL 1 beta , and IL 8 mRNA expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Normal cells were incubated with LPS , gamma interferon ( IFN gamma ) , or tumor necrosis factor ( TNF alpha ) alone or in combination . ^^^ Results showed that PMN release of both NO and O 2 was reduced in septic samples ; in contrast , an association of LPS , IFN gamma , and TNF alpha promoted maximal NO release by normal cells ( 40 50 % ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Addition of antibodies against interleukin 3 ( IL 3 ) , granulocyte / macrophage colony stimulating factor ( GM CSF ) , or tumor necrosis factor alpha ( TNF alpha ) to the media had little or no effect on the ability of the cytokines to affect oxidation by macrophages , whereas anti interferon gamma ( IFN gamma ) antibodies completely reversed the inhibition induced by the T cell supernatant . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Finally , TNF alpha production in response to IL 2 and sCD 23 precedes IFN gamma and IFN gamma secretion is significantly inhibited by anti TNF alpha mAb , indicating that the sCD 23 costimulatory signal for IFN gamma production may be partially mediated by TNF alpha release . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Before and for one week after drug administration , concentrations of interleukin 1 ( IL 1 ) , IL 2 , IL 4 , IL 6 , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) , produced by anti CD 3 stimulated peripheral blood mononuclear cells , were measured . ^^^ Piroxicam treatment resulted in elevation of levels of IL 2 , depression of IL 1 , IL 6 , TNF alpha and IFN gamma , and no consistent effect on IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of CEA on cultured endothelial cells can be enhanced by TNF alpha or IFN gamma , and decreased by IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Of IL 2 , IL 4 , IL 5 , IL 6 , IL 10 , IFN gamma , and TNF alpha , only IFN gamma mRNA is appreciably and reproducibly increased in irradiated , IL 2 activated T cells ( T cells * rIL2 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When compared to TCC derived from the PB of both patients and controls , RO TCC showed remarkably high proportions of both CD8+ and CD4+ clones with a Th 1 like cytokine profile , as documented by their ability to secrete IL 2 , IFN gamma , and tumor necrosis factor alpha ( TNF alpha ) , but not IL 4 or IL 5 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The modulation of cell growth by tumor necrosis factor alpha ( TNF alpha ) , or TNF alpha in combination with transforming growth factor beta ( TGF beta ) or interferon gamma ( IFN gamma ) was investigated . ^^^ The combination of TNF alpha and TGF beta in U 937 cells and that of TNF alpha and IFN gamma in NA cells cooperatively reduced the expression of c myc mRNA . ^^^ These data suggest that the reduction of c myc gene at transcriptional level by TNF alpha or TNF alpha in combination with TGF beta or IFN gamma plays a primary role in the inhibition of cell growth at an early stage . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mitogenic stimulation with irradiated Daudi lymphoma cells and phytohemagglutinin led to a strong release of interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) and interleukin 2 ( IL 2 ) by T cells in patients with chronic hepatitis and in healthy controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Several cytokines interleukin 2 ( IL 2 ) , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) and prostaglandin E 2 ( PGE 2 ) known to modulate tumour growth and metastasis were examined to determine whether they regulate nm 23 expression in JAR in vitro . ^^^ No effect was found in the JAR cell line , whereas TNF alpha , IFN gamma and PGE 2 down regulated nm 23 expression in the B16F10 cell line . ( ABSTRACT TRUNCATED AT 400 WORDS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma induced production of TNF alpha and of reactive nitrogen intermediates as well as mycobacterial growth inhibition were inhibited by exogenous IL 10 , but only when given prior to IFN gamma stimulation . ^^^ We conclude that the outcome of mycobacterial infection is regulated by a coordinate interplay between IFN gamma , TNF alpha and IL 10 . . ^^^ Growth inhibition of Mycobacterium bovis by IFN gamma stimulated macrophages : regulation by endogenous tumor necrosis factor alpha and by IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Additional stimulation of LPS treated monocytes with interferon gamma ( IFN gamma ) as a priming agent enhanced TNF alpha formation in stable function patients and , in contrast , slightly reduced monokine formation in chronic rejection patients , which suggests that in this group a high activation level of monocytes has already been reached in vivo by T cell factors such as IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The upregulation of ICAM 1 by IL 1 alpha , TNF alpha , and IFN gamma appeared similar , whereas the induction of VCAM 1 by IL 1 alpha , IL 4 , TNF alpha , and IFN gamma showed differences between the three cell types . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When T cells of these mice were activated by a single injection of anti CD 3 epsilon monoclonal antibody , mice died of interstitial pneumonitis at 24 48 h after injection , accompanied by elevation of serum levels of TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Decreased mRNA levels for IL 1 beta , TNF alpha , IL 6 , IL 10 , and IFN gamma as compared with untreated , hemorrhaged controls were present in intraparenchymal pulmonary mononuclear cells following therapy with anti TGF beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The early role of IFN gamma is unknown , but might include enhancing antigen presentation , inducing secretion of tumor necrosis factor alpha and helping early precursor macrophages to express nonspecific listericidal activity . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Transcription of TNF alpha gene in astrocytes stimulated by NDV or LPS and IFN gamma was inhibited completely by the tyrosine kinase inhibitor herbimycin , and partially by a PKC inhibitor H 7 , as determined by nuclear run on assay . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The responsiveness to either IFN gamma , TNF alpha , or IL 1 beta treatment was shown to vary from cell line to cell line , indicating complex mechanisms that regulate the expression of ICAM 1 at both , the transcriptional and the post transcriptional level . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , stimulation of eosinophils with interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , or interleukin 3 ( IL 3 ) markedly upregulated ICAM 1 surface expression in a time and dose dependent manner . ^^^ Accordingly , unstimulated eosinophils did not express significant amounts of ICAM 1 mRNA , but ICAM 1 mRNA expression could be markedly induced in these cells upon stimulation with IFN gamma plus TNF alpha . ^^^ The combination of TNF alpha with either IFN gamma , IL 3 , IL 5 , or granulocyte / macrophage colony stimulating factor ( GM CSF ) increased ICAM 1 expression in a synergistic fashion , whereas IL 5 or GM CSF by itself did not induce ICAM 1 expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The basal level adhesion ( 20 35 % ) was significantly up regulated by treating EC with recombinant murine gamma interferon ( IFN gamma ) , interleukin 1 alpha ( IL 1 alpha ) and / or tumor necrosis factor alpha ( TNF alpha ) . ^^^ Pretreatment of EC with TGF beta also partially inhibited the up regulation of adhesion induced by IFN gamma , IL 1 alpha and / or TNF alpha . ^^^ TGF beta had no effect on the up regulation of ICAM 1 expression induced by IFN gamma , IL 1 alpha and / or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cells were then assessed over time for the capacity to accumulate transcripts for IL 1 alpha , IL 1 beta , IL 2 , IL 3 , IL 4 , IL 5 , IL 6 , IFN gamma , TNF alpha , and TNF beta ; to secrete IL 2 , IL 3 , IL 4 , IL 5 , IL 6 , and IFN gamma ; and to progress through S phase . ^^^ Before the first major cell division in culture ( < 32 h ) , stimulated CD4+ cells of the old group contained similar peak levels of IL 2 , TNF alpha , and TNF beta transcripts relative to young adult controls , whereas IL 3 , IL 4 , IL 5 , and IFN gamma transcripts accumulated to significantly higher peak levels in the old group . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of ICAM 1 in myocardial cells could be induced in vitro by IFN gamma and TNF alpha , which were shown to be synthesized by the infiltrating cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although IL 5 , TNF alpha , and IFN gamma had no stimulatory effect in the presence of hCD40L alone , if IL 4 was also present in cultures , these cytokines enhanced the proliferative response above that seen with IL 4 alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We demonstrate that IFN gamma production from SCID splenocytes is stimulated by interleukin ( IL ) 12 , tumor necrosis factor alpha ( TNF alpha ) , and IL 2 but is inhibited by IL 10 , IL 10 , IL 12 , and TNF are induced by heat killed Listeria monocytogenes ( hk LM ) from SCID splenocytes and peritoneal macrophages . ^^^ TNF alpha and IL 2 act synergistically with IL 12 to augment IFN gamma production . ^^^ In contrast , IL 10 inhibits hk LM induced IFN gamma production at two levels : ( 1 ) by inhibiting TNF and IL 12 production from these cultures ( presumably from the macrophage ) and ( 2 ) by inhibiting the stimulatory effects of IL 12 and TNF alpha on NK cell IFN gamma production . ^^^ Thus , these data indicate that macrophage production of TNF alpha and IL 12 stimulates the release of IFN gamma by NK cells and that IL 10 produced in response to hk LM inhibits this response at the level of the macrophage and the NK cell . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Incubation of HUVE or HSE with IL 1 or TNF in combination with interferon gamma ( IFN gamma ) greatly potentiated the increase in ICAM 1 surface expression . ^^^ The synergistic effect of IFN gamma and TNF was confirmed by several methods , including a cell based enzyme linked immunosorbent assay , fluorescence activated cell sorting , immunofluorescence staining , and determination of mRNA levels . ^^^ IFN gamma also augmented the actions of several other agonists on HSE , i . e . , IL 4 , lipopolysaccharide , and TNF beta / lymphotoxin . ^^^ Immunoprecipitation of TNF alpha + IFN gamma stimulated , 125I labeled HSE cells with anti ICAM 1 revealed a single 90 kd band , similar in size to ICAM 1 from HUVE treated in an identical manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1 beta , TNF alpha and IFN gamma potentiated ICAM 1 expression in both cell lines in a dose related manner . ^^^ ICAM 1 expression induced by IL 1 beta , rabbit IL 1 beta 208 240 and human IL 1 beta 208 240 was blocked by the IL 1ra , while TNF alpha and IFN gamma induced ICAM 1 expression were not . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Given our recent observation that DS thymuses overexpress mRNA for IFN gamma and TNF , and the fact that both of these cytokines induce ICAM 1 expression on cultured human thymic epithelial cells , we propose that increased levels of IFN gamma and TNF contribute to the enhanced expression of ICAM 1 in DS thymuses . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin 2 ( IL 2 ) activated effector cells could enhance ADCC activity , but interferon gamma ( IFN gamma ) or tumour necrosis factor alpha ( TNF alpha ) had no effect on the cytotoxic activity of effector cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Addition of cytokines such as IL 1 alpha , IL 2 , IL 4 , IL 6 , GM CSF , IFN gamma , tumor necrosis factor ( TNF alpha , or TNF beta ) , or neutralizing Ab to these cytokines had no effect on SAg induced death of Ag specific TCL . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Endothelial cells from human umbilical veins were isolated and examined for CAM expression and its modulation by tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 ( IL 1 ) , interleukin 6 ( IL 6 ) or interferon gamma ( IFN gamma ) . ^^^ The expression of intercellular adhesion molecule 1 ( ICAM 1 ) on HUVEC was increased by TNF alpha , IL 1 and IFN gamma when measured by ELISA or flow cytometric ( FACS ) analysis . ^^^ Two melanoma cell lines , Malme 3M and SK Mel 28 , showed increased expression of ICAM 1 after treatment with TNF alpha , IL 1 and IFN gamma in FACS analysis . ^^^ HUVEC and melanoma cells did not express LFA 1 and increased ICAM 1 expression by TNF alpha , IL 1 and IFN gamma treatment in FACS analysis did not coincide with minimal increase of melanoma cells adhesion to cytokine treated HUVEC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The aggregate formation correlated with a dramatic rise in ICAM 1 expression and an increased production of IFN gamma , TNF alpha , and IL 1 beta , molecules associated with regulation of ICAM 1 expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This effect is independent from other induced cytokines , because it is not prevented by antibodies neutralizing interferon ( IFN ) alpha , IFN beta , IFN gamma , IL 2 or tumor necrosis factor ( TNF ) alpha and , unlike the induction of IFN gamma production by peripheral blood lymphocytes , it does not require HLA class 2 positive accessory cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha increases IFN gamma but not LPS induced NO 2 production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 10 is a potent inhibitor of monocyte macrophage activation , that inhibits production of tumor necrosis factor alpha ( TNF alpha ) , IL 1 and also IFN gamma from lymphocytes acting at the level of accessory cells . ^^^ Because TNF alpha and IL 1 are not efficient inducers of IFN gamma , the mechanism by which IL 10 inhibits IFN gamma production is not clear . ^^^ Our findings that antibodies against NKSF / IL 12 , TNF alpha , or IL 1 beta can significantly inhibit IFN gamma production in response to various stimuli and that NKSF / IL 12 and IL 1 beta can overcome the IL 10 mediated inhibition of IFN gamma , suggest that IL 10 inhibition of IFN gamma production is primarily due to its blocking production from accessory cells of the IFN gamma inducer NKSF / IL 12 , as well as the costimulating molecule IL 1 beta . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Most cytokines upregulated ICAM 1 expression ( IL 1 beta > TNF alpha > IFN gamma > > PDGF bb , IL 6 ) , but not GM CSF or TGF beta . ^^^ Kinetic studies of ICAM 1 expression differed among several cytokines : an early rise with IL 1 beta or TNF alpha stimulation , a gradual increase with IFN gamma , a transient increase with PDGF bb , and a plateau with IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We also excluded that production of TNF alpha , IFN gamma , or IL 2 by the IL 12 activated effectors might account for the cytotoxicity inducing effects of this cytokine . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Thus , LPS activation of HUVE for PMNL transendothelial migration : ( a ) does not require secretion of IL 1 , TNF alpha or IL 8 by the endothelium , ( b ) IFN gamma enhances and prolongs endothelial activation by LPS and may increase leucocyte infiltration in LPS or bacterial inflammatory reactions , and ( c ) CD 18 dependent mechanisms are equally important for PMNL transendothelial migration under both acute ( 3 hr ) and prolonged ( 22 hr LPS + IFN gamma ) activation of endothelium . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human IL 10 ( hIL 10 ) is a newly described cytokine that was originally identified as a cytokine synthesis inhibitory factor regulating the production of several pro inflammatory cytokines such as IL 1 alpha , IL 1 beta , IL 6 , IL 8 , TNF alpha , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Because IFN gamma can not be responsible for all cytokine effects during recovery from MCMV infection we have now tested the potential participation of tumour necrosis factor alpha ( TNF alpha ) in the antiviral defence . ^^^ These data suggest that the antiviral effect of the CD 4 subset requires the presence of at least two cytokines , namely IFN gamma and TNF alpha . ^^^ Furthermore , endogenous cytokines were found to be required for survival after infection with lethal doses of MCMV , whereas immunotherapy with recombinant TNF alpha and IFN gamma could not limit virus replication in vivo . ^^^ The results suggest that , similar to IFN gamma , TNF alpha is an integral part of the protective mechanisms involved in cytomegalovirus clearance . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Antiviral activity of IFN gamma and TNF has been demonstrated in vitro and in vivo . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effect of the chemotactic cytokine , IL 8 , on neutrophil function was compared with that of of other cytokines , GM CSF , G CSF TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 6 , IL 8 , IFN gamma , TNF alpha were expressed in normal heart tissue at low level and were upregulated following ischemia and reperfusion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We now demonstrate that IFN gamma and tumour necrosis factor alpha ( TNF alpha ) display synergism in their antiviral activity . ^^^ As little as 2 ng / ml of IFN gamma and TNF alpha reduced the virus yield by about three orders of magnitude . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the effects of tumour necrosis factor alpha ( TNF alpha ) , interleukin 1 alpha ( IL 1 alpha ) , interleukin 2 ( IL 2 ) and interferon gamma ( IFN gamma ) on the immune response elicited by inactivated rabies virus vaccine in a mouse model . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Whether immune and inflammatory responses within the CNS are propagated or suppressed depends on a number of parameters , including ( a ) the activational status of these cells , ( b ) cytokine receptor levels on glial and immune cells , ( c ) the presence of cytokines with both immune enhancing and immune suppressing effects ( IFN gamma , IL 1 , TNF alpha , IL 6 , TGF beta , CsFs ) , ( d ) the concentration and location of these cytokines in the CNS , and ( e ) the temporal sequence in which a particular cell is exposed to numerous cytokines ( see Fig . 1 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In order to better examine the sequence of events leading to lung injury in this setting , we investigated lung histology as well as in vivo mRNA levels for cytokines with proinflammatory and immunoregulatory properties ( IL 1 beta , IL 6 , IL 10 , TNF alpha , TGF beta , IFN gamma ) over the 3 days following hemorrhage and resuscitation . ^^^ Significant increases in mRNA levels for IL 1 beta , IL 6 , IL 10 , and IFN gamma , but not TNF alpha , were present among intraparenchymal pulmonary mononuclear cells obtained 1 and 3 days after hemorrhage . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We studied the effect of tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 ( IL 1 ) , and interferon gamma ( IFN gamma ) on the function of thyroid cells and pituitary thyrotrophs . ^^^ IL 1 and IFN gamma did not cause a further reduction of TNF alpha induced inhibition of [ 125I ] iodide transport . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Toxoplasma gondii : acquired ocular toxoplasmosis in the murine model , protective role of TNF alpha and IFN gamma . ^^^ Treatment of mice with mAbs against T cells ( CD 4 plus CD 8 ) or cytokines ( IFN gamma or TNF alpha ) resulted in a marked increase of ocular lesions , more often associated with the presence of the parasite and the severity of inflammatory response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In a further series of experiments , we demonstrate that SEB causes the secretion of a variety of cytokines ( IL 1 , 2 , 4 , 10 , granulocyte macrophage CSF , IFN gamma , and TNF ) that may cause lethal septic shock . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation of IL 2 activated NK cells with the mAb triggered TNF alpha and IFN gamma production , which was enhanced by using the mAb attached to plastic or in the presence of suboptimal concentrations of phorbol esters . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The ability of T cell lines derived from tumor infiltrating lymphocytes ( TIL ) from patients with ovarian carcinoma to produce interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and IL 6 in response to autologous or allogeneic ovarian carcinoma tumor cells was determined . ^^^ Certain of these T cell lines ( 3 of 7 ) exhibited antigen independent ( spontaneous ) production of moderate to low concentrations of IFN gamma ( 75 to 112 pg / ml ) , TNF alpha ( 62 88 pg / ml ) , and IL 6 ( 38 to 690 pg / ml ) , in culture medium alone in the absence of rIL 2 , tumor cells or polyclonal activators . ^^^ Addition of 20 IU / ml of rIL 2 to the cultures resulted in a significant increase of the antigen independent production of IFN gamma ( range 75 6480 pg / ml ) by all seven T cell lines and TNF alpha ( 44 490 pg / ml ) by 5 of 7 T cell lines . ^^^ Certain TIL derived T cell lines ( 2 of 7 ) produced IFN gamma , TNF alpha , and IL 6 in response to stimulation with autologous ovarian tumor cells , in the presence of 20 IU / ml of rIL 2 . ^^^ Irradiated autologous ovarian tumor cells alone or in the presence of rIL 2 did not produce any detectable levels of IFN gamma , TNF alpha , or IL 6 during the 6 day culture . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Vesnarinone inhibited the production of TNF alpha and IFN gamma both in healthy volunteers and in patients with heart failure . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , TNF alone is not sufficient to cause death , instead synergizing interactions with cytokines like IL 1 , IL 6 , and IFN gamma are probably involved . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Whereas the localized and sustained release of TNF and IFN gamma mediated by CD4+ cells seems to be the focusing event triggering mononuclear cell accumulation , the coincidental eradication of bacteria critically depends on CD8+ and / or CD 4 CD8 Thy1+ cells . ^^^ In addition , the increased production of cytokines characteristic of an anamnestic response ( IL 2 , IL 3 , IL 4 , IFN gamma and TNF ) can be dramatically reduced by depleting CD4+ T cells without any effect on the animal ' s ability to eradicate high lethal doses of bacteria and Listeria specific CD8+ T cells can mediate protection even in the presence of neutralizing antibodies to IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The most dramatic responses were seen in the mRNA levels of IL 1 alpha , IL 1 beta , IL 6 , TNF alpha , and IFN gamma in iris / ciliary body . ^^^ Lesser mRNA level responses were found for IL 1 beta and IL 6 in cornea and for IL 1 alpha , IL 1 beta , IL 6 , TNF alpha , and IFN gamma in neuroretina . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To investigate the role of the inflammatory cytokines , the cerebrospinal fluid concentrations of interleukin ( IL ) 1 beta , tumour necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) were measured in 11 children with bacterial meningitis and two with mycoplasmic meningoencephalitis and compared with those in 50 children with aseptic meningitis and 15 with non pleocytotic cerebrospinal fluid . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF ' s stimulatory activity on fibroblast NGF production was synergized by interleukin 1 alpha ( IL 1 alpha ) , IL 1 beta and interferon gamma ( IFN gamma ) , but was antagonized by transforming growth factor beta ( TGF beta ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Induced levels of interleukin 1 beta ( IL 1 beta ) , IL 2 , tumor necrosis factor alpha , and gamma interferon ( IFN gamma ) were compared among groups of individuals representing three phases of immunity to infection with M . tuberculosis : naive normal control subjects , purified protein derivative ( PPD ) reactive normal donors , and individuals with active tuberculosis ( TB [ diseased ] ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To define the possible role of cytokines , we studied the effect of direct administration of LPS ( 600 micrograms / mouse , IP ) , tumor necrosis factor ( TNF , 2 . 5 or 50 micrograms / mouse , 4 ) , interleukin 1 ( IL 1 beta , 2 . 5 micrograms / mouse , 4 ) , interferon gamma ( IFN gamma , 2 . 5 micrograms / mouse , 4 ) , or their combination at 2 . 5 micrograms each . ^^^ LPS induced pulmonary edema was partially inhibited by anti IFN gamma antibodies but not by anti TNF antibodies , anti IL 1 beta antibodies , or IL 1 receptor antagonist ( IL 1Ra ) . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor ( TNF ) alpha appeared to be a cofactor for IFN gamma induction by MLA . ^^^ With low doses of MLA ( < 5 micrograms ) , IFN gamma induction was dependent upon exogenous TNF alpha administered either in advance of or with MLA . ^^^ A 25 micrograms dose of MLA induced significant IFN gamma accumulation in the absence of exogenous TNF alpha . ^^^ In this case , endogenous TNF alpha appeared to be a cofactor in the response , since suppression of TNF alpha production with dexamethasone inhibited IFN gamma induction , and this inhibition was overcome by administration of exogenous TNF alpha with MLA . ^^^ Tolerant animals did not produce IFN gamma when challenged with LPS , and this tolerance was not abrogated by supplementing mice with exogenous TNF alpha during the challenge . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The following cytokines failed to induce IL 1ra production : IL 2 , IL 6 , G CSF , M CSF , IFN gamma , TGF beta 1 , TGF beta 2 , TNF alpha , acidic and basic FGF , PDGF and EGF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Data suggest that although the EIA kits that were evaluated for human IL 1 alpha , IFN gamma , and TNF beta failed , the EIA kits for IL 1 beta , IL 2 , IL 4 , IL 6 , and TNF alpha , the bioassays and RT PCR assays for each of the cytokines were successful in detection and most likely quantitation of the non human primate cytokine homologues . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma , TNF , and IL 6 production by vaccinia virus immune spleen cells . ^^^ TNF , IL 6 , and IFN gamma were detected within 12 h of restimulation with maximal levels reached by 24 h . ^^^ Adherent cells were major producers of IL 6 and TNF , whereas IFN gamma production was dependent on CD4+ T cells and adherent cells . ^^^ The IFN gamma response was Ag specific , whereas the production of TNF and IL 6 was not . ^^^ Stimulation with vaccinia virus constructs encoding IFN gamma or TNF altered the levels of cytokines produced , in that IFN gamma expression by stimulator cells led to increased IFN gamma production , whereas TNF expression by stimulator cells augmented both TNF and IFN gamma production by responder cells . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF and IL 1 , produced in response to LPS , promoted Fc alpha R increase , as did GM CSF ; whereas IFN gamma down regulated Fc alpha R . ^^^ An increase in Fc alpha R specific mRNA was detected in monocytes treated with TNF , IL 1 , GM CSF , and LPS ; whereas message was reduced in cells treated with IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma greatly increased LPS mediated TNF alpha production by human neutrophils ( p < 0 . 01 ) , and SNP plus NAC was found to further augment this production ( p < 0 . 01 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cells were cultivated in the presence of IFN gamma and LPS for up to 48 h and changes in the secretion of nitric oxide ( NO . ) and tumor necrosis factor alpha ( TNF alpha ) were observed as activation markers . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
HIV 1 antigen stimulation of PBMC did not induce the intracellular accumulation of interleukin 6 ( IL 6 ) , tumour necrosis factor alpha ( TNF alpha ) , or interferon gamma ( IFN gamma ) . ^^^ However , cells stimulated with L . donovani antigens exhibited the production of IL 6 and TNF alpha , but not IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neither parasitaemia nor plasma levels of tumour necrosis factor alpha ( TNF alpha ) , IL 6 , lymphotoxin ( LT ) , interferon gamma ( IFN gamma ) , IL 4 , soluble IL 4R or soluble CD 8 differed significantly between the two groups of children with malaria . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We observed that Sendai virus preinduction of peripheral blood mononuclear cells and subsequent mitogenic stimulation resulted in : ( 1 ) Superproduction of interferon gamma , ( IFN gamma ) ( 2 ) an increase in interleukin 2 ( IL 2 ) synthesis that correlates with DNA synthesis when stimulated with phytohemagglutinin ( PHA ) or pokeweed mitogen ( PWM ) after treatment with the Sendai virus , while stimulation with Protein A from Staphylococcus aureus was not affected , and ( 3 ) enhanced tumor necrosis factor alpha ( TNF alpha ) production in response to bacterial lipopolysaccharide ( LPS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both approaches showed minimal evidence of protein secretion for interleukin 4 ( IL 4 ) , IL 5 , and tumor necrosis factor , while IL 2 , IL 10 , and gamma interferon ( IFN gamma ) were prominent throughout the response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To obtain information about useful combinations of various cytokines in melanoma therapy , we studied the influence of interleukin 2 ( IL 2 ) in combination with interferon alpha ( IFN alpha ) , IFN gamma and tumour necrosis factor alpha ( TNF alpha ) on the lytic activity of IL 2 stimulated cells in vitro . ^^^ IFN alpha , IFN gamma and TNF alpha did not enhance lytic activity with an optimal IL 2 dose ( 50 IU / ml IL 2 ) . ^^^ The specific lysis of M 19 cells was significantly increased by pretreatment of the cells with 5 IU / ml IFN alpha together with 50 IU / ml TNF alpha ( t test , P < or = 0 . 001 ) , while the specific lysis of M 26 cells was increased by pretreatment with 5 IU / ml IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There are overlapping activities of the cytokines TNF alpha , IL 1 , IFN gamma and IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Effect of IFN gamma and endogenous TNF on the histopathological changes in the liver of Listeria monocytogenes infected mice . ^^^ In the present study , the effect of interferon gamma ( IFN gamma ) on the development of these lesions , and the involvement of endogenous tumour necrosis factor alpha ( TNF alpha ) in the IFN gamma induced effects were evaluated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There were no differences in the IFN alpha , IFN gamma or TNF alpha levels between groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
None of the other cytokines tested ( IL 1 alpha , IL 6 , TNF alpha , IFN alpha , IFN gamma ) supported growth of NK 92 cells which have the following characteristics : surface marker positive for CD 2 , CD 7 , CD11a , CD 28 , CD 45 , CD 54 , CD56bright ; surface marker negative for CD 1 , CD 3 , CD 4 , CD 5 , CD 8 , CD 10 , CD 14 , CD 16 , CD 19 , CD 20 , CD 23 , CD 34 , HLA DR . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro incubation of FHCM or the W 1 cell line for varying periods with varying concentrations of IFN gamma , TNF alpha , Poly IC , LPS , IL alpha , IL 1 beta , PMA , PDBu , and supernatant fluids from Con A activated PBMC or allogeneic MLR cultures failed to induce cell adhesion molecules ( CAMs ) or costimulatory molecules that are not constitutively expressed on these cells except for MHC class 2 antigens . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study we show that primary rat osteoblast like cells as well as the clonal rat osteoblast like cell line UMR 106 , stimulated with IFN gamma together with TNF alpha and LPS , produce NO , measured as nitrite production . ^^^ IL 1 alpha enhanced while TGF beta 2 inhibited TNF alpha + IFN gamma + LPS stimulated NO production in UMR 106 cells dose dependently . ^^^ The competitive inhibitor of NO production , NG monomethyl arginine ( L NMMA ) , and cycloheximide abolished the increase in nitrite production induced by TNF alpha + IFN gamma + LPS , while hydrocortisone had no effect , as previously reported for chondrocytes . ^^^ Furthermore , we found that in cultured fetal mouse long bone explants the combination of TNF alpha + IFN gamma + LPS as well as the NO donor sodium nitroprusside could inhibit osteoclastic resorption , measured as 45Ca release . ^^^ Histological evaluation revealed that the TNF alpha + IFN gamma + LPS induced inhibition of 45Ca release was associated with a decrease in the number of tartrate resistant acid phosphatase positive osteoclasts . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In order to investigate the relationship between the production of 90K and soluble inflammatory mediators , we studied serum concentrations of the antigen , tumour necrosis factor alpha ( TNF alpha ) , interleukin 1 alpha ( IL 1 alpha ) , interferon gamma ( IFN gamma ) , IFN alpha , neopterin and beta 2 microglobulin ( beta 2 m ) in patients with non neoplastic HIV infection at various stages of disease and in control persons . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Thus , depressed TNF synthesis , diminished expression of CD 14 , and low plasma LPS binding capacity , in addition to blocked IFN gamma signaling in the mutant mice , likely to combine to manifest in the resistant phenotype of IFN gamma R / mice to endotoxin . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines , interleukin ( IL ) 4 , IL 6 , interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , soluble CD 23 ( sCD 23 ) , and soluble IL 2 receptors ( sIL 2R ) are mediators of inflammation and immune response . ^^^ The authors measured the circulating levels of IL 4 , IL 6 , IFN gamma , TNF alpha , sCD 23 , and sIL 2R from patients with T cell chronic lymphocytic leukemia ( T CLL ) , T cell acute lymphoblastic leukemia ( T ALL ) and peripheral T cell lymphoma ( PTCL ) to determine their importance in these T cell disorders . ^^^ IL 4 , IL 6 , IFN gamma , TNF alpha , sCD 23 , and sIL 2R levels were measured from the serum samples by enzyme linked immunosorbent assay or bioassay methods . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In R . conorii infected mice depleted of gamma interferon ( IFN gamma ) and / or tumor necrosis factor alpha ( TNF alpha ) by intravenous administration of neutralizing monoclonal antibodies on days 0 , 2 , and 4 , the mortality rate was 100 % . ^^^ Assay of liver infiltrated by lymphocytes and macrophages revealed mRNA of IFN gamma and TNF alpha , indicating that the host defenses were activated at the site of infection . ^^^ These observations support the hypothesis that IFN gamma secreted by T lymphocytes and natural killer cells and TNF alpha secreted by macrophages act in a synergistic , paracrine fashion on adjacent rickettsia infected endothelial cells , hepatocytes , and macrophages to stimulate synthesis of nitric oxide , which kills intracellular R . conorii . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The CTL incubated in the presence of the HLA A2+ SCCHN PCI 50 or 13 , but not an HLA A2+ gastric carcinoma , produced TNF alpha , IFN gamma and GM CSF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Surface density of APO 1 and the fraction of positive cells in HT 29 was enhanced by interferon gamma ( IFN gamma ) and , additively , by tumor necrosis factor alpha ( TNF alpha ) , whereas in SW 480 APO 1 expression was not modulated by these cytokines . ^^^ We conclude that neoplastic transformation of colon epithelium often leads to a loss of the physiologic , high level of surface APO 1 by giving rise either to a stable lack of APO 1 or to an IFN gamma / TNF alpha sensitive phenotype of inducible APO 1 expression . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
For this purpose , we studied the effect of 5 cytokines ( IFN alpha , IFN beta , IFN gamma , TNF alpha and TGF beta ) , used as single agents or in combination , on 4 ovarian cancer cell lines which present different antigenic profiles with the monoclonal antibodies ( MAbs ) tested ( OC 125 , OVTL 3 , MOv 18 and MOv 19 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
An additive effect of interferon gamma ( IFN gamma ) was observed on LPL induced TNF alpha mRNA expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In all donors tested , optimal doses of rhIL 2 induced transcription of genes encoding for IL 5 , GM CSF , IFN gamma , and TNF alpha whereas transcription of IL 1 alpha , IL 3 , IL 4 , and IL 6 genes could only be detected in about half of the donors . ^^^ Interestingly , rhIL 10 dose dependently inhibited the production of IFN gamma and TNF alpha induced by IL 2 , but had no effects on PBMC proliferation and generation of LAK activity . ^^^ On the other hand , rhIL 10 shows diverse effects on rhIL 2 triggered PBMC activation , in that it inhibits IFN gamma and TNF alpha production but does not affect PBMC proliferation or generation of LAK activity . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine analysis of the supernatants from MLPR indicates that there is significantly higher production of TNF alpha , IFN gamma , and IL 2 in supernatants from the abortion prone combination than in supernatants from the normal combination . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
LIF synthesis was significantly increased by the cytokines IL 1 beta , TNF alpha , and TGF beta 1 , but not by IFN gamma , IL 6 , or LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have shown that , although tolerant in vivo , animals bearing long term cultured islet allografts are donor reactive in vitro as assessed by ( 1 ) CTL precursor frequency , ( 2 ) antidonor proliferative and cytotoxic responses , and ( 3 ) lymphokine production ( IL 2 , IL 3 , TNF , and IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Antibodies against IFN gamma and TNF alpha significantly inhibited SIVsmmPBj / anti class 2 induced formation of MGCs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition TNF beta was induced in 3 / 10 and IFN gamma in 5 / 10 non responders . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Well characterized human glomerular mesangial cells were stimulated in vitro with interferon gamma ( IFN gamma ) , interleukin 1 , and tumor necrosis factor alpha ( TNF alpha ) to determine the influence of these cytokines on the expression of class 1 and 2 HLA antigens . ^^^ TNF alpha induced only class 1 antigens when used alone , but acted synergistically with IFN gamma in the expression of class 1 and 2 antigens . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neutralization of endogenously produced IL 10 by administration of 2 mg JES 5 2A5 anti IL 10 monoclonal antibody ( mAb ) 2 h before LPS challenge resulted in a marked increase in both TNF and IFN gamma serum levels while irrelevant isotype matched mAb had no effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
De novo expression of TNF , IFN gamma , IL 3 , IL 4 , and IL 6 genes was initiated rapidly by treatment of mice with anti CD 3 . ^^^ In contrast , in vivo and in vitro anti TNF treatment upregulated anti CD 3 induced IFN gamma mRNA expression and inhibited IL 4 mRNA expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increased levels of IL 2 , IL 4 , IL 6 , IL 10 , TNF alpha , transforming growth factor beta , and IFN gamma were observed , with similar levels of expression in TOL compared with REJ liver . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In our experiments Zn2+ ions , added as ZnSO 4 , stimulated PBMC to produce IFN gamma , IL 1 beta , IL 6 , TNF alpha , and sIL 2R in a concentration dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using digoxigenin labelled oligonucleotide probes we have detected expression of the cytokines IL 1 alpha , IL 2 , IL 4 , IL 6 , IL 10 , IFN gamma , TGF beta 1 & 2 and TNF alpha in frozen sections of CNS tissue from MS cases . ^^^ The intensity and distribution of the staining for mRNA is cytokine specific , IL 6 , IFN gamma and TNF alpha predominating in the perivascular inflammatory cuffs , the others being more weakly expressed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A protocol using combined exposure to interferon gamma ( IFN gamma ) , calcitriol and tumour necrosis factor alpha ( TNF alpha ) has been reported to activate human monocytes in vitro to kill Mycobacterium tuberculosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using reverse transcriptase and a quantitative PCR to measure cytokine mRNA , we found Sjgren ' s syndrome : 1 ) salivary gland CD4+ T cells produce over 40 fold more IL 2 , IFN gamma , and IL 10 mRNA than peripheral blood CD4+ T cells from the same patient or from normal controls ; 2 ) salivary gland CD4+ T cells produced little IL 4 and IL 5 mRNA immediately after elution from the salivary gland , although these mRNAs could be induced by mitogen stimulation of Sjgren ' s syndrome salivary gland lymphocytes in vitro ; 3 ) salivary gland epithelial cells produced over 40 fold more IL 1 alpha , IL 6 , and TNF alpha mRNA than epithelial cells from individuals with histologically normal salivary glands . ^^^ Increased levels of IL 1 alpha , IL 6 , IL 10 , TNF alpha , and IFN gamma cytokines were found by ELISA assay in the saliva of Sjgren ' s syndrome patients , indicating that the elevated mRNA levels detected in their glandular tissue by PCR correlate with local protein synthesis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Simultaneous addition of IFN gamma and LPS increased TNF release for at least 8 h . ^^^ TNF plus IL 1 induced the greatest HbNO generation , followed by TNF plus IFN gamma , and then IL 1 plus IFN gamma . ^^^ These results suggest that increase of TNF release by IFN gamma plays a key role in NO generation in LPS treated rats . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These results indicate that IFN gamma is capable of down regulating the production of TNF alpha by a local macrophage population . ^^^ The ability of IFN gamma to modify the production of TNF alpha is dependent on the timing of administration relative to LPS exposure . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Peripheral blood mononuclear cells ( PBMC ) from infants with CMA were cultured in the presence of cow ' s milk proteins ( CMP ) , and the release of tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and interleukin 4 and 6 was measured . ^^^ Recombinant human TNF alpha altered the HT 29 cl . 19A epithelial barrier capacity , and its effect was highly potentiated by IFN gamma . ^^^ CONCLUSIONS : These results indicate that during CMA , the high level of TNF alpha released by mononuclear cells after milk protein challenge acts synergistically with IFN gamma to increase the intestinal permeability . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Biopsies were taken before IFN gamma , after IFN gamma , before TNF alpha and between 2 hr and 60 days after the TNF alpha perfusion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cooperative effects of TNF alpha and IFN gamma are determining factors in the ability of IL 10 to protect mice from lethal endotoxemia . ^^^ Treatment with a combination of neutralizing antibodies against TNF alpha and interferon gamma ( IFN gamma ) resulted in complete protection . ^^^ Neutralizing antibodies against TNF alpha and IFN gamma were protective when administered alone to normal BDF 1 mice , as previously demonstrated in C . parvum primed mice . ^^^ These findings suggest that lethal endotoxemia is a result of the cooperative activities of TNF alpha and IFN gamma in normal mice of the BALB / cJ and BDF 1 strains as well as in C . parvum primed BDF 1 mice . ^^^ IL 10 appears to be less effective in protecting mice from lethal endotoxemia when cooperation between IFN gamma and TNF alpha is facilitated by high level production of the cytokines as in C . parvum primed mice or when there is evidence of strong synergy between them as in normal BALB / cJ mice . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In RAW 264 . 7 macrophages , MIF secretion also was induced by tumor necrosis factor alpha ( TNF alpha ) and IFN gamma , but not by interleukins 1 beta or 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , a 48 hour pretreatment of MC with either rm IFN gamma alone or in combination with rh TNF alpha and / or the continuous presence of rm IL 1 alpha during coculture periods from 72 to 144 hours substantially enhanced the proliferative LN response . ^^^ FACS analysis of these adherent LN after detachment demonstrated that in comparison to cocultures with untreated MC , cocultures of LN with IFN gamma / TNF alpha pre treated MC resulted in a 24 . 4 % increase of Thy1 . 2+ cells , with 89 % of these being L3T4+ T helper lymphocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
First , we found that administration of 1000 U of recombinant mouse IL 10 ( mIL 10 ) 30 min before injection of 10 micrograms of the 145 2C11 antimouse CD 3 mAb markedly reduced the systemic release of IFN gamma and TNF . ^^^ While hIL 10 was as efficient as mIL 10 in reducing TNF and IFN gamma release , it did not modify peak serum levels of IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Addition of IL 4 , tumour necrosis factor ( TNF ) alpha , interferon ( IFN ) alpha , or IFN gamma resulted in a profound inhibition of SEM growth . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1 , IL 6 and TNF were produced by human monocytes stimulated for 2 h , IFN gamma positive lymphocytes were detected later . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Spleen cells from mice pretreated with AIL every 2 days for 1 week produced interleukin 2 ( IL 2 ) , interferon gamma ( IFN gamma ) , tumor necrosis factor ( TNF ) and interleukin 6 ( IL 6 ) when these cells were stimulated in vitro by AIL . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study , ciprofloxacin was shown to increase the levels of mRNA for IL 1 alpha , IL 2 and its receptor , IFN gamma , IL 3 , IL 4 , granulocyte macrophage / CSF , TNF alpha , and lymphotoxin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Jar cells were incubated for 24 h with varying concentrations ( 5 10 10 ( 4 ) 40 micrograms / ml ) of the following cytokines : Il 1 , Il 2 , Il 3 , Il 4 , Il 5 , Il 6 , IFN gamma , tumor necrosis factor ( TNF ) alpha , M CSF and GM CSF . ^^^ Trophoblast viability in the presence of TNF alpha ( 10 micrograms / ml ) and IFN gamma ( 40 micrograms / ml ) was only 40 % and 50 % , respectively . ^^^ Il 1 appears to have a stimulatory effect on trophoblast hCG release , while TNF alpha and IFN gamma appear to have cytotoxic effects on trophoblast cells . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To investigate the possible role of cytokines in the mediation of glomerular injury in the nephrotic syndrome , the levels of interleukin ( IL ) 1 beta , IL 2 , interferon ( IFN ) alpha , IFN gamma , and tumor necrosis factor alpha ( TNF alpha ) were measured in patients with primary nephrotic syndrome . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , if mice were treated prior to infection with a combination of murine IFN gamma and murine TNF alpha , the dose of IFN gamma required to achieve significant reduction in mortality was reduced by a factor > 10 . ^^^ In contrast to the beneficial effects of prophylactic TNF alpha treatment in combination with IFN gamma , TNF alpha treatment of mice after MCMV infection resulted in increased mortality . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The anti cancer drugs examined in this set were 13 in total : ADM , AMR , BLM , MMC , VCR , VLB , etoposide , CDDP , 5 FU , MTX , IFN alpha , IFN gamma and TNF . ^^^ On the other hand , that to MTX was observed in 1 patient at the lowest rate ( 5 % ) , and that to the BRM group drugs was observed in 3 in IFN alpha ( 14 % ) , 1 in IFN gamma ( 5 % ) and 2 in TNF ( 9 % ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokines , interleukin 1 ( IL 1 ) , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) and IL 4 , increased production of M CSF above constitutive levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The release of both 55 and 75 kDa TNF R was stimulated when PA 1 cells were cultured with IL 1 beta and IFN gamma but unaffected by IL 4 . ^^^ IFN gamma was the most potent stimulator of receptor release particularly of the 75 kDa TNF R . ^^^ IFN gamma also induced increased expression of cell membrane TNF R measured by binding of 125I labeled TNF . ^^^ Membrane TNF Rs which were induced by IFN gamma were the 75 kDa type , because TNF binding was blocked by anti 75 kDa TNF R antibody . ^^^ These data suggest that IFN gamma selectively induced release and expression of 75 kDa TNF Rs . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These include interleukins 1 , 2 and 3 ( IL 1 , 2 and 3 ) , interferon gamma ( IFN gamma ) , ovine trophoblast protein ( oTP 1 ) , tumour necrosis factor alpha ( TNF alpha ) and granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The mechanism by which IL 4 and IL 10 acted to inhibit DTH responses did not appear to be through inhibition of IFN gamma or tumor necrosis factor production as treatment with antibodies which neutralized these activities failed to inhibit DTH responses . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Standard `` wounds ' ' were established in confluent monolayers of the intestinal cell line IEC 6 , and migration was quantitated in the presence or absence of the physiologically relevant cytokines transforming growth factor ( TGF ) alpha , epidermal growth factor ( EGF ) , interleukin ( IL ) 1 beta , IL 6 , tumor necrosis factor ( TNF ) alpha , interferon gamma ( IFN gamma ) , and platelet derived growth factor ( PDGF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To obtain a better understanding of the immune response to Epstein Barr virus ( EBV ) , we measured the cytokines tumour necrosis factor ( TNF ) alpha / beta , interleukin 2 ( IL 2 ) , interferon gamma ( IFN gamma ) , IL 6 and granulocyte macrophage colony stimulating factor ( GM CSF ) in the conditioned medium of peripheral blood mononuclear cells from 10 healthy adults before and at 48 h and at 1 , 2 , 3 and 4 weeks following infection in vitro with EBV . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , interferon gamma ( IFN gamma ) , a potent activator of TNF alpha expression , failed to overcome the inhibition of TNF alpha caused by beta glucan . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Soluble interleukin 2 receptor ( sIL 2R ) , interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) levels were evaluated in serum from patients affected by myasthenia gravis ( MG ) . sIL 2R titers were significantly increased in generalized and bulbar MG patients while ocular cases were not different from controls . ^^^ IFN gamma was not detected in serum of both MG patients and healthy subjects while TNF alpha levels were not statistically different from controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha transcripts were present in both the alloAP+ and alloAP M theta subsets , but IL 1 alpha and IL 6 were not constitutively expressed by the alloAP+ subset of M theta ; rather , they were induced by IFN gamma , which was released from naive CD8+ T cells only during coculture with alloAP+ M theta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The levels of NO released were elevated further after stimulation of cells with T cell mitogens or specific parasite Ag ; antibody blocking experiments demonstrated that this up regulation of NO synthesis was at least partially dependent upon IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we investigated the kinetics of LPS induced sTNFR release and the role of the cytokines TNF , leukemia inhibiting factor , IFN gamma , and IL 1 in this process . ^^^ Pretreatment with anti IFN gamma mAb before LPS results in a significant reduction in TNF and sTNFR P 55 levels , but sTNFR P 75 levels are significantly increased . ^^^ The influence of TNF , leukemia inhibiting factor , IL 1 , and IFN gamma on the kinetics of LPS induced circulating sTNFR is discussed in the context of the pathophysiology of LPS induced disease . . ^^^ Investigation of the role of tumor necrosis factor , IL 1 , leukemia inhibiting factor , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , no effect of TGF beta could be demonstrated on macrophages stimulated with IFN gamma plus tumor necrosis factor alpha ( TNF alpha ) under the same conditions . ^^^ Interestingly , when macrophages were pretreated with TGF beta for 24 h , NO 2 production in response to IFN gamma plus TNF alpha was also inhibited . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although LPS activated M phi underwent marked loss of cytotoxicity within 24 hr following initiation of in vitro culture , this 20 hr pretreatment with IFN gamma or TNF alpha inhibited this reduction in tumoricidal effects in a dose dependent fashion . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increases in levels of IL 1 alpha , IL 6 , tumor necrosis factor ( TNF alpha ) and interferon ( IFN gamma ) were associated with an increase in the survival of tumor bearing mice treated with IL 7 and / or LH . ^^^ These results suggest that changes in T cell , NK cell and cytokines such as IL 1 alpha , IL 6 , TNF alpha and IFN gamma in response to IL 7 and / or LH might account for prolonged survival of B16a melanoma bearing mice and that IL 7 might be useful as a potential antitumor agent combined with other therapy in certain malignant solid tumors with metastases . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
On the other hand , there was no difference between BB / Sendai and Wistar rats in the in vivo TNF / LT productivity induced with LPS or with IFN gamma plus LPS , and the TNF / LT productivity of these rats was lower on stimulation with LPS alone , but higher with IFN gamma plus LPS than the other normal rats . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We also demonstrated that the addition of POF led to a POF dose dependent decrease of the release of the cytokines interleukin ( IL ) 2 , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These include cytokines such as IFN gamma , IFN alpha / beta , and TNF alpha , known to enhance class 1 expression . ^^^ The supernatant class 1 enhancing factor is not IFN gamma , IFN alpha , or TNF alpha because MV infected cells did not produce measurable levels of these cytokines as detected by immunoassay or polymerase chain reaction . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
HUVEC which had been preincubated with LPS , interleukin 1 alpha ( IL 1 alpha ) , IL 1 beta , tumor necrosis factor ( TNF alpha ) , or interferon gamma ( IFN gamma ) produced more PGI 2 than control cells in response to thrombin . ^^^ However , the HUVEC preincubated with the conditioned medium made with mononuclear cells with or without LPS ( LPS Mo CM , Mo CM ) produced more PGI 2 than those preincubated with LPS , IL 1 alpha , IL 1 beta , TNF alpha , or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Plasma levels of interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) were determined in healthy individuals and patients with aplastic anemia ( ApAn ) . ^^^ Spontaneous production of IFN gamma and TNF alpha by cultured bone marrow mononuclear cells was observed in four of seven patients but not in the six healthy controls ( P = 0 . 026 ) . ^^^ Spontaneous production of IFN gamma and TNF alpha by cultured peripheral blood mononuclear cells from patients and controls was however similar . ^^^ Phytohemagglutinin ( PHA ) induced production of IFN gamma and TNF alpha by cultured mononuclear cells did not differ significantly between ApAn patients and normal controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pretreatment of the cells for 24 h with 50 U / ml recombinant human tumor necrosis factor alpha ( TNF alpha ) or 5 IU / ml of human interferon gamma ( IFN gamma ) resulted in only modest reduction ( 2 to 19 fold ) in virus yield . ^^^ We conclude that the combination of TNF alpha and IFN gamma at low concentrations can exert a powerful anti herpes effect in human corneal fibroblasts which can be chiefly attributed to the induction of IFN beta . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have investigated the effects of the pro inflammatory cytokines interleukin 1 beta ( IL 1 beta ) , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) on the iron metabolism of the human monocytic cell line U 937 . ^^^ IL 1 beta , TNF alpha and IFN gamma all decreased transferrin iron uptake into cells , and all three cytokines had effects on the proportion of iron associated with ferritin . ^^^ Transferrin receptor expression was diminished by TNF alpha and IL 1 beta , but not IFN gamma , suggesting different effector mechanisms . ^^^ Both TNF alpha and IFN gamma increased the amount of cellular mRNA for ferritin H chain , but not the L chain ; IL 1 beta affected mRNA for neither ferritin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Thus a macrophage , under the influence of at least IFN gamma and TNF alpha , can progress from being a nonlistericidal cell to one that can kill the intracellular organism . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Samples of human milk contained sizable amounts of IL 6 , IL 8 and IFN gamma ; yet the liquid phase was consistently negative for other cytokines ( i . e . , TNF alpha , IFN alpha / beta , IL 1 alpha / beta ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Secretion of lymphokines ( IFN gamma , TNF alpha and GM CSF ) was induced by contact of the biMAb coated effector cells with the relevant tumor target , whereas the soluble biMAb was virtually ineffective . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MBP mRNA expression in HuH 7 cells was increased by interleukin 6 ( IL 6 ) , dexamethasone and heat shock , decreased by interleukin 1 ( IL 1 ) , and unaffected by interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) and transforming growth factor beta ( TGF beta ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To shed light on the mechanisms of U 937 MAK and its decrease during HIV infection , IL 1 beta , IL 6 , TNF alpha , GM CSF , and IFN gamma production was analyzed . ^^^ Decreases in TNF alpha , GM CSF , and IFN gamma , but not IL 1 beta or IL 6 , levels were observed in MP F 2 stimulated PBMC from HIV infected subjects , compared to healthy controls . ^^^ Interestingly , anti IFN gamma antibodies also abolished TNF alpha production , and the anti TNF alpha antiserum effect was comparable to that of anti IFN gamma in U 937 MAK inhibition . ^^^ In contrast , anti TNF alpha antibodies abrogated TNF alpha activity , but only partially reduced IFN gamma production . ^^^ Furthermore , the present results indicate a pivotal role for IFN gamma in U 937 MAK activity , possibly through activation of TNF alpha production . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : We examined the in vitro production of interleukins ( IL 2 , IL 4 , IL 6 ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) in purified peripheral blood mononuclear cells ( PBMCs ) of patients with hepatitis B virus positive ( HBV ) , acute viral hepatitis ( A HBV ) , HBV + chronic active hepatitis ( HBV CAH ) , and autoimmune type chronic active hepatitis ( AI ACH ) . ^^^ RESULTS : IFN gamma and TNF alpha production were characteristically higher in patients with A HBV than in healthy control subjects ( p < 0 . 001 ) . ^^^ The cytokine profile ( PBMC induced IL 2 , IL 4 , IL 6 , IFN gamma , and TNF alpha production ) is different in A HBV , HBV CAH , and AI CAH disease . ^^^ The increased cytokine secretion ( IFN gamma and TNF alpha in A HBV and IL 4 and IL 6 in AI CAH ) could reflect altered relative frequencies of different cell phenotypes in these diseases . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of TNF receptor ( TNFR ) mRNA has been examined in murine peritoneal macrophages stimulated with LPS and / or IFN gamma . ^^^ Interestingly , though IFN gamma can synergize with LPS for the expression of TNF alpha , it abrogated the LPS mediated enhancement of type 2 TNFR in a dose dependent fashion . ^^^ These findings suggest that IFN gamma and LPS modulate the physiologic action of TNF through complex mechanisms involving effects on the transcription of TNF alpha itself and on receptors through which it may act in autocrine or paracrine fashion . . ^^^ IFN gamma and lipopolysaccharide differentially modulate expression of tumor necrosis factor receptor mRNA in murine peritoneal macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
C 215 SEA mediated T cell killing of C 215 expressing tumor cells irrespective of their expression of MHC class 2 antigens and induced levels of IFN gamma and TNF in mononuclear cells sufficient to completely suppress the growth of colon carcinoma cells in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytotoxic factor lysed autologous and allogeneic freshly isolated human tumor cells , while they were resistant to any of recombinant cytokines , including tumor necrosis factor ( TNF ) , lymphotoxin ( LT ) , interferon ( IFN ) alpha , IFN gamma , interleukin ( IL ) 1 alpha and IL 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , the effect of cytokines tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) on the programmed cell death of thymocytes was examined by measuring DNA fragmentation and LDH release . ^^^ TNF alpha and IFN gamma had no effect on DNA fragmentation in control and TPA , or A 23187 treated thymocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Recent evidence suggests the existence of at least two pathways of acquired specific resistance to M . tuberculosis infection : The first consists of cytokine mediated activation of infected host cells by protective CD4+ T cells , which are currently believed to mediate antimicrobial activity via the release of macrophages activating cytokines such as IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Prevention of resistance to IFN alpha antiproliferative activity : characterization of the effect of IFN gamma and substitution for IFN gamma by tumor necrosis factor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) , interleukin 1 beta ( IL 1 beta ) , and tumor necrosis factor alpha ( TNF alpha ) were strong macrophage PCA inducers . ^^^ PCA induced by IFN gamma , IL 1 beta , and TNF alpha depended largely on tissue factor expression , as evidenced by experiments with factor 10 deficient plasma and antitissue factor antibodies . ^^^ In macrophages subcultured in adherence , IL 1 beta was a strong PCA inducer , whereas IFN gamma and TNF alpha promoted little PCA increase . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It is well known that interferon gamma ( IFN gamma ; type 2 ) potentiates various responses of human tumor necrosis factor ( TNF ) in a wide variety of cells and that this potentiation is accompanied by the up regulation of TNF receptor synthesis . ^^^ Exposure of these cells to IFN gamma led to a decrease in TNF receptor number ( 4029 vs . 2719 sites / cell ) without any change in the receptor affinity ( 0 . 96 nM vs . 1 . 1 nM ) . ^^^ Like IFN gamma , IFN alpha and IFN beta ( type 1 ) down modulated the TNF receptors on these cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In euthymic mice , levels of TNF alpha , IL 1 alpha , IL 6 , and IL 1 beta mRNA in the brain increased early in infection and further increased with the progression of disease ; the level of IFN gamma mRNA was expressed at high levels late in infection and correlated with the onset of clinical disease . ^^^ In euthymic mice , in situ hybridization revealed IFN gamma , TNF alpha , and IL 1 alpha expressing cells throughout the inflammatory cell infiltrates associated with areas of LCMV nucleic acid . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this report , we show that transforming growth factor beta ( TGF beta ) can significantly inhibit the capacity of IFN gamma , IL 1 beta , and TNF alpha to augment expression of the central component of complement C 3 in the human astroglioma cell line D 54 MG . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To study mechanisms of antibiotic effects in typhoid fever , levels of interleukin 6 ( IL 6 ) , gamma interferon ( IFN gamma ) , and cytokine receptors ( tumor necrosis factor receptor [ TNF R ] p 55 and TNF R p 75 ) were measured in the plasma of 29 adult Nepalese with culture positive typhoid fever before therapy and on days 4 and 15 after start of therapy with either ceftriaxone at 2 g / day for 3 days or chloramphenicol at 50 mg / kg of body weight per day for 14 days . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study we found that addition of a range of doses of interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) , or granulocyte macrophage colony stimulating factor ( GM CSF ) to cultures of bone marrow derived murine macrophages infected with the 25291 strain of Mycobacterium avium gave rise to varying degrees of bacteriostasis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The induction of L tryptophan degradation was specific for IFN gamma since neither IFN alpha , IFN beta , IL 1 , IL 2 , IL 6 , GM CSF nor TNF alpha induced the production of detectable amounts of kynurenine by 86HG39 and 2D9 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokines tumor necrosis factor ( TNF ) , beta interferon ( IFN beta ) , and IFN gamma increase major histocompatibility complex class 1 molecule expression . ^^^ In electromobility shift assays , a p 50 containing NF kappa B nuclear factor from TNF treated cells binds kappa B containing probes , and ISGF 2 from IFN gamma treated cells binds ICS containing probes . ^^^ A probe containing both the kappa B and ICS elements ( kappa B ICS ) forms a novel complex with nuclear factors isolated from cells treated with both TNF and IFN gamma ; this complex also forms when nuclear factors from individually cytokine treated cells are mixed in vitro . ^^^ The natural variant ICS found in HLA A responds to IFN gamma and can mediate synergy with TNF . ^^^ However , the variant kappa B found in HLA C does not respond to TNF , nor can it mediate synergy between TNF and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
By itself TNF alpha induced a release of small amounts of neopterin but strongly potentiated the effect of IFN gamma in a synergistic manner to induce neopterin secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To assess the effect of lymphoid cytokines on trypomastigote viability , 10 ( 5 ) freshly harvested parasites were cultured with a wide range of dilutions of human recombinant IL 1 , IL 3 , IL 6 , interferon gamma ( IFN gamma ) or tumor necrosis factor alpha ( TNF alpha ) , or bovine recombinant IFN gamma or TNF alpha for 24 , 48 or 96 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In these prelesional areas , interleukin 1 ( IL 1 ) , IL 2 receptor ( IL 2R ) and tumour necrosis factor ( TNF ) were detected in the cytoplasm of the infiltrating cells , whereas no detectable reactivity was noted for IL 2 , IL 6 , interferon gamma ( IFN gamma ) or lymphotoxin ( LT ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study we used both in vivo and in vitro murine models to investigate the effects of cocaine on the release of cytokines ( IL 1 alpha , IL 6 , and TNF alpha by peritoneal macrophages and splenocytes , IL 2 , IL 4 , IL 5 , IL 10 , and IFN gamma produced by splenocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha , as well as interferon gamma ( IFN gamma ) or interleukin 6 ( IL 6 ) had a significant up regulating effect on U 937 expression of Fc gamma RI / CD64 . ^^^ Furthermore , the action of TNF alpha was augmented by IL 6 , and more evidently by IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The results outlined below show that rhIL 10 strongly inhibits lectin induced production of IFN gamma , TNF alpha , IL 2 , and to a lesser degree proliferation and IL 4 production of both T cell subsets . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The pattern of chemoattractant cytokine gene ( IP 10 , JE , KC ) expression has been examined in BALB / c 3T3 fibroblasts and MC3T3 E 1 osteoblasts in response to a variety of different inflammatory stimuli , including IFN gamma , TNF alpha , IL 1 alpha , and IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , LAK cells released IFN gamma , IL 1 beta , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Administration of low doses of 100 U interferon gamma ( IFN gamma ) plus 10 U tumor necrosis factor alpha ( TNF alpha ) to the tumor bearers , a combination treatment that we previously showed to diminish the presence of GM suppressor cells synergistically , restored proliferative responsiveness of the splenic T cells to IL 2 . ^^^ ER MP12+ cells composed approximately 10 % of the cells isolated from dissociated tumors of mice that had been treated with placebo or with either IFN gamma or TNF alpha alone , but IFN gamma / TNF alpha therapy markedly reduced the number of tumor infiltrating ER MP12+ suppressor cells . ^^^ The IFN gamma / TNF alpha treatment to eliminate GM suppressor cells and restore T cell responsiveness to IL 2 was next coupled with low dose IL 2 therapy ( 100 U twice daily ) . ^^^ Addition of IL 2 to the treatment regimen did not significantly influence the effectiveness of the IFN gamma / TNF alpha treatment in eliminating GM suppressor cells from the LLC LN 7 tumor mass . ^^^ However , inclusion of IL 2 with the IFN gamma / TNF alpha treatment regimen enhanced the CD8+ , but not the CD4+ , cell content within the tumor , and diminished the number of metastatic lung nodules within the mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Within 2 hours after injection of SEB , serum tumor necrosis factor alpha ( TNF alpha ) levels reached a peak , followed by high levels of serum interferon gamma ( IFN gamma ) up to 10 hours after injection . ^^^ Passive immunization with anti TNF alpha / beta neutralizing monoclonal antibody ( mAb ) protected GalN sensitized mice from the lethal effects of SEB , with less protection with anti IFN gamma neutralizing mAb . ^^^ SEB induced the production of TNF alpha and IFN gamma in a dose dependent manner from splenic mononuclear cells in vitro . ^^^ CONCLUSIONS : The results show that SEB contributes to lethal shock associated with severe hepatic injury in GalN sensitized mice and suggest that TNF alpha and IFN gamma produced in response to SEB may be mediators of the lethal toxicity and hepatotoxicity of SEB . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using dual stain immunohistochemistry , we initially compared the ability of a mixture of cytokines ( IL 1 alpha , IL 1 beta , IL 2 , IL 6 , IL 8 , TNF alpha , TNF beta , IFN beta and IFN gamma ) with that of physical stimuli such as heat shock and peroxide , to increase cellular expression of the mainly inducible hsp 72 species in mixed glial cell cultures ( containing ODC , astrocytes and microglia ) . ^^^ When used individually , however , only IL 1 alpha ( 79 + / 3 % ) , IFN gamma ( 70 + / 2 % ) and TNF alpha ( 65 + / 5 % ) induced ODC hsp 72 expression in mixed glial cell cultures . ^^^ An IL 1 receptor antagonist ( IL 1ra ) , abrogated hsp 72 induction by IL 1 alpha ( 16 + / 3 % ) as well as that due to IFN gamma ( 14 + / 1 % ) and TNF alpha ( 13 + / 2 % ) in mixed glial cell cultures . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum concentrations of interleukin 1 ( IL 1 ) , IL 2 , IL 6 , interferon alfa ( IFN alpha ) , IFN gamma , tumor necrosis factor alpha ( TNF alpha ) and GM CSF as well as the cytokine dependent serum components neopterin , beta 2 microglobulin ( B2M ) , C reactive protein ( CPR ) , soluble IL 2 receptor ( sIL 2R ) and 2 ' 5 ' oligoadenylate synthetase ( 2 5 OA ) were assayed using ELISAs and RIAs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Alternately , increase in Ye 2 reactivity was also observed when the L cells were incubated with IFN gamma or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Consistent with this hypothesis , treatment of microglia with TNF alpha or IL 6 ( in the presence or absence of IFN gamma ) inhibited ( p < 0 . 01 ) , in a dose dependent manner , T . gondii growth . ^^^ Results of our study suggest that the host defense activity of human microglia against T . gondii is dependent primarily on the activating properties of IFN gamma , TNF alpha , and IL 6 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Patients were given for a period of 15 days on alternating days injections of IFN beta ( IFN beta ser ) , 90 or 450 10 10 ( 6 ) IU , IFN gamma , 0 . 1 100 10 10 ( 6 ) IU , or TNF 125 275 micrograms / m2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Gamma interferon ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and interleukin 1 ( IL 1 ) markedly stimulate the release of MCP 1 as measured by a specific and sensitive radioimmunoassay . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We also found that , in contrast to AM , IM did not respond synergistically to combinations of IFN gamma and lipopolysaccharide ( LPS ) or tumor necrosis factor alpha in terms of nitric oxide production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunocytochemical staining of spinal cords from five autopsied patients with HTLV 1 associated myelopathy / tropical spastic paraparesis was performed using a panel of monoclonal or polyclonal antibodies reactive with interleukin 1 beta ( IL 1 beta ) , interleukin 6 ( IL 6 ) , tumor necrosis factor ( TNF ) alpha , interferon ( IFN ) alpha , IFN beta , IFN gamma and transforming growth factor ( TGF ) beta . ^^^ In the spinal cords of patients with a shorter duration of illness , IL 1 beta , TNF alpha , and IFN gamma were expressed on perivascular infiltrating macrophages , astrocytes and microglia in active chronic inflammatory lesions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The pterins neopterin and xanthopterin have been measured together with the cytokines IFN gamma , TNF alpha and TNF beta in the supernatants of 20 allogeneic mixed lymphocyte reactions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
B / I expanded T cells from five of six patients secreted the cytokines tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) in response to co culture with autologous tumour cells but not with irrelevant tumour . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have investigated the in vitro effects of interleukin 1 beta ( IL 1 beta ) , interleukin 2 ( IL 2 ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) on the induction of anti thyroperoxidase ( anti TPO ) , anti thyroglobulin ( anti Tg ) , and IgG secreting cells ( SC ) by cultures of pokeweed mitogen ( PWM ) stimulated peripheral blood mononuclear cells ( PBMC ) obtained from patients with Hashimoto ' s thyroiditis ( HT ) , Graves ' disease ( GD ) , and controls ( C ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To understand the differential role of interferon gamma ( IFN gamma ) and interleukin 4 ( IL 4 ) in the process of macrophage tumoricidal activation , we investigated the production of tumor necrosis factor alpha ( TNF alpha ) and nitric oxide in activated murine macrophages and the effects of those lymphokines on prostaglandin E 2 ( PGE 2 ) mediated down regulation . ^^^ IFN gamma and IL 4 increased lipopolysaccharide ( LPS ) induced TNF alpha production by different mechanisms because IL 4 , unlike IFN gamma , failed to overcome the LPS hyporesponsiveness in C3H / HeJ mice . ^^^ Although treatment of macrophages with IFN gamma and IL 4 gave rise to a similar enhancing effect on macrophage TNF alpha production , these two lymphokines appeared to differentially regulate the overall functional state of macrophages for tumour cell killing capability . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neutralization of endogenous TNF production in vivo resulted in an amelioration of clinical GVHD , but neutralization of endogenous IFN gamma resulted in a more severe course . ^^^ However , in vivo neutralization of either TNF or IFN gamma post BMT resulted in a decreased ability of splenocytes from animals with GVHD to suppress mitogen responses but did not affect the generation of the suppressor cell population . ^^^ These findings support multiple roles for TNF and IFN gamma in the pathophysiology of GVHD , including terminal cellular differentiation and / or regulation of effector cell function . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro and in vivo studies suggest that combinations of cytokines such as interferon gamma or interferon alpha ( IFN gamma , IFN alpha ) and tumor necrosis factor alpha ( TNF alpha ) may act synergistically . ^^^ Treatment with combinations of IFN alpha / TNF alpha or IFN gamma / TNF alpha , or with MAb G 250 alone , resulted in a significant inhibition of tumor growth . ^^^ Treatment with MAb G 250 , in combination with IFN gamma / TNF alpha , did not result in an improve anti tumor effect as compared to that of either treatment alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Recombinant IL 10 and melanoma supernatants were found to inhibit production of TNF alpha , IFN gamma , IL 2 and mixed lymphocyte reactions but reversal of these effects of melanoma supernatants by MAbs against IL 10 was only seen in the case of TNF alpha production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Comparative analysis of tumor infiltrating lymphocyte ( TIL ) and peripheral blood lymphocyte ( PBL ) on IFN gamma and TNF alpha production in gynecological cancers ] . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This human leukocyte antigen restricted process induces not only lysis of target cells but also secretion of lymphokines by the CTLs , including TNF alpha , TNF beta , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We demonstrated with high reproducibility the expressions of messenger RNAs of cytokines , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) in the lung tissue of M . pulmonis infected mice . ^^^ Next , mRNAs prepared from the lung tissues of M . pulmonis infected and uninfected mice were tested for the presence of messages specific to TNF alpha and IFN gamma by the reverse transcriptase polymerase chain reaction . ^^^ The expression of the genes encoding TNF alpha and IFN gamma was constitutively demonstrated from 24h through 35 days after the intranasal inoculation of M . pulmonis . ^^^ Furthermore , cells of two types , adherent and nonadherent cells , in bronchoalveolar lavage fluids obtained from the mice 3 weeks after inoculation of M . pulmonis were found also to express the genes of TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The production of the cytokines IFN gamma , IL 1 alpha , IL 2 and TNF alpha was investigated in mitogen stimulated , whole blood cell culture from 239 untreated patients with primary gynaecological carcinomas ( breast , cervix , ovary , endometrium ) , and 191 healthy female controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We could demonstrate the presence of the cytokines IFN gamma , IL 2 , tumour necrosis factor alpha ( TNF alpha ) and TNF beta in the supernatants harvested during the generation of BAK cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
With IFN alpha at 500 IU / ml , tumor necrosis factor alpha ( TNF alpha ) and IFN gamma were detected at markedly high levels for 2 24 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Induction of surface tumor necrosis factor ( TNF ) expression and possible facilitation of surface TNF release from human monocytic cells by granulocyte macrophage colony stimulating factor or gamma interferon in combination with 1 , 25 dihydroxyvitamin D 3 . 1 , 25 dihydroxyvitamin D 3 ( 1 , 25 ( OH ) 2D3 ) , gamma interferon ( IFN gamma ) and granulocyte macrophage colony stimulating factor ( GM CSF ) can regulate monocyte maturation and activation . ^^^ GM CSF and IFN gamma combined with 1 , 25 ( OH ) 2D3 increased cellular TNF secretion to levels not seen with these agents alone . ^^^ The combination of 1 , 25 ( OH ) 2D3 and GM CSF , however , facilitated lipopolysaccharide ( LPS ) mediated release of surface TNF from U 937 cells , an effect that was temporally independent of maximal maturation . 1 , 25 ( OH ) 2D3 plus IFN gamma was less effective than 1 , 25 ( OH ) 2D3 plus GM CSF at facilitating TNF secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines presently known to be involved in systemic bacterial infection are tumour necrosis factor ( TNF ) , interleukin ( IL ) 1 , IL 6 , IL 8 and interferon gamma ( IFN gamma ) and the counterregulatory molecules soluble TNF receptor ( sTNFR ) and IL 1 receptor antagonist ( IL 1 Ra ) . ^^^ In animal models TNF , IL 1 and IFN gamma mediate organ damage , low blood pressure and fatality , whereas IL 6 is involved in infection related manifestations , like the production of acute phase protein and fever , and IL 8 is chemotactic to granulocytes . ^^^ In experiments as well as in human infection TNF , IL 1 , IL 6 , IL 8 and IFN gamma have been detected , and levels of TNF , IL 6 and IL 8 have been found to be associated with the severity of the disease . ^^^ Collectively , TNF , IL 1 and IFN gamma emerge as mediators of systemic infection and septic shock whereas IL 6 and IL 8 are related to other manifestations of infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have studied the effects of interferons gamma ( IFN gamma ) and alpha ( IFN alpha ) , and tumour necrosis factor alpha ( TNF ) on circulating 14 day erythroid progenitor cell ( BFU E ) growth in vitro from patients with non leukaemic myeloproliferative disorders ( MPD ) compared with normal controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human recombinant ( hr ) interleukin 1 alpha / beta ( IL 1 alpha / beta ) , hr tumor necrosis factor alpha ( TNF alpha ) , and hr interferon gamma ( IFN gamma ) each increased HUVEC monolayer permeability in a time and dose dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma and IL beta production by the LAK cells was enhanced by stimulation with the Caki 1 , ACHN and K 562 tumor cell lines , while TNF alpha production was stimulated by Caki 1 and K 562 cells . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The in vitro response of freshly isolated peripheral blood mononuclear cells from patients with B cell chronic lymphocytic leukemia ( B CLL ) and a high white cell count , to four cytokines , IFN alpha , IFN gamma , IL 2 and TNF , was studied . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PURPOSE : We assayed serum interleukin 1 beta ( IL 1 beta ) , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) levels in children with virus associated hemophagocytic syndrome ( VAHS ) to evaluate the relationship of these cytokines to other laboratory parameters . ^^^ PATIENTS AND METHODS : Interleukin 1 beta , TNF alpha , and IFN gamma were measured in 42 serial sera from six children with VAHS using a sensitive radioimmunoassay . ^^^ RESULTS : The cytokine IFN gamma or TNF was detected in 19 / 19 ( 100 % ) or 11 / 19 ( 69 % ) samples , respectively , during the active febrile phase , at much higher levels than during the remission phase of the disease ( IFN : p < 0 . 00001 ; TNF : p < 0 . 01 ) . ^^^ Serial cytokine examination revealed that the elevated TNF and IFN gamma levels decreased rapidly to an undetectable level in concert with clinical improvement after an effective treatment , and that IFN gamma , but not TNF , was detected just before a relapse marked by spiking fever . ^^^ Although TNF and IL 1 were not statistically linked to these parameters , TNF levels were related to IFN gamma levels ( product moment CC : 0 . 546 , p < 0 . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It was shown that all the Ts cell clones produced IL 2 , IL 4 , IFN gamma , TGF beta 1 , LT , and TNF alpha upon activation with hamster anti CD 3 monoclonal antibody ( mAb ) or antigen plus APC . ^^^ However , neither the mAbs to IFN gamma , TGF beta , or LT / TNF alpha , nor the recombinant IL 2 was able to abrogate the suppression of in vitro antibody production by cloned Ts cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
While interferon gamma ( IFN gamma ) is present in EAN nerves and may act as a local stimulus for TNF expression , the nature of this signal in Wallerian degeneration in the absence of IFN gamma is unknown . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunomodulatory properties of PTX were not only associated with blockage of TNF alpha , as shown by decreased mRNA expression and TNF alpha values in the culture supernatants , but also with an impaired production of other cytokines and secondary messages such as IFN gamma and neopterin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The growth of C 6 glioma cells was stimulated by TNF alpha , IFN gamma and IL 6 but not by TNF beta . ^^^ However , TNF alpha and IFN gamma but not IL 6 induced the synthesis of NO in C 6 cells . ^^^ Moreover , N monomethyl L arginine a competitive inhibitor of NO synthase blocked TNF alpha and IFN gamma dependent proliferation and NO induction on C 6 cells , but had no effect on IL 6 dependent proliferation . ^^^ In addition , C 6 proliferation induced by TNF alpha and IFN gamma was specifically blocked by inhibitors of cyclic nucleotide dependent protein kinases such us H 9 . ^^^ Those results suggest that TNF alpha and IFN gamma but not IL 6 induce the growth of glia cells through the generation of NO which in turns activate a cyclic nucleotide dependent kinase . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have measured the levels of interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 alpha ( IL 1 alpha ) , IL 1 beta , and IL 2 in the whole blood cell culture supernatants of 43 tumor patients undergoing a treatment with biological response modifiers or a conventional therapy with 5 fluorouracil and leucovorin . ^^^ In the blood cell cultures of the 16 patients who received 5 fluorouracil and leucovorin IFN gamma levels decreased ( P < or = 0 . 01 ) and TNF alpha levels rose ( P < or = 0 . 05 ) during each therapy cycle . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In an effort to determine the molecular events associated with IFN alpha induction in this system , freshly isolated human monocytes were primed by culture with either IFN gamma or granulocyte macrophage colony stimulating factor and then treated with LPS ; expression of IFN alpha subtype 2 ( IFN alpha 2 ) , IFN regulatory factors ( IRFs ) , and TNF was assessed by Northern ( RNA blot ) analysis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Biological assays to measure interleukin 2 ( IL 2 ) , IL 4 , gamma interferon ( IFN gamma ) , and tumor necrosis factor alpha or tumor necrosis factor beta and Northern ( RNA ) blot analysis to verify the expression of IL 2 , IL 4 , IFN gamma , and tumor necrosis factor alpha revealed differential production of cytokines by the Th cell clones . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These data indicate that the resistance of SCID mice to C . parvum infection is IFN gamma dependent , whereas tumor necrosis factor appears not to play a significant role . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Three human cell lines derived from Ewing ' s sarcoma ( RM 82 , VH 64 , and WE 68 ) were investigated to establish the influence of recombinant human interferon gamma ( rhIFN gamma ) and tumour necrosis factor alpha ( rhTNF alpha ) on cell proliferation and survival and to characterize IFN gamma and TNF alpha receptor expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this model , the protective effect of anti IFN gamma mAb contrasted with the ineffectiveness of a neutralizing anti TNF alpha mAb . ^^^ These findings suggest that overproduction of IFN gamma might have a more detrimental role than overproduction of TNF alpha during focal gram negative infections . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The present study shows that various cytokines such as tumor necrosis factor ( TNF alpha ) , interleukin 1 alpha ( IL 1 alpha ) , and interferon gamma ( IFN gamma ) make tumor cells much more susceptible to the cytostatic 5 ' deoxy 5 fluorouridine ( 5 ' dFUrd ) than to 5 fluorouracil ( 5 FUra ) and other cytostatics . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In our study , using microglial / neuronal cell cocultures primed with IFN gamma , we found that both LPS and TNF alpha triggered neuronal cell injury ( impairment of gamma aminobutyric acid uptake and neuronal loss ) via a nitric oxide mechanism . ^^^ The mechanism by which IL 4 exerts its neuroprotective effect was found to involve the inhibition of IFN gamma priming of microglia with a subsequent decrease in the production of TNF alpha and nitric oxide . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum interferon gamma ( IFN gamma ) was also elevated in patients with high TNF alpha levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To assess the effects of cytokines on 1 alpha hydroxylase activity , blood monocytes were cultured in the presence and absence of human recombinant tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and interleukins 1 and 2 and then incubated with 25 D 3 substrate . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study , we show that mice treated in vivo with Corynebacterium parvum are primed for the production of interferon gamma ( IFN gamma ) and exhibit an enhanced capacity to produce serum IL 1 alpha , TNF alpha , and IL 6 when challenged intravenously with lipopolysaccharide ( LPS ) . ^^^ Pretreatment with a rat antimouse TNF alpha monoclonal antibody ( mAb ) protected 90 % of the animals against the lethal endotoxin challenge , while an anti IFN gamma mAb gave approximately 75 % protection . ^^^ The anti IFN gamma mAb also caused a reduction in LPS induced serum TNF alpha and IL 1 alpha . ^^^ These results indicate that TNF alpha and IFN gamma are major mediators of endotoxin shock in C . parvum treated mice . ^^^ The results further suggest that the IFN gamma produced by C . parvum primed mice in response to an LPS challenge serves as a stimulus for enhanced production of TNF alpha and IL 1 alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin 1 alpha ( IL 1 alpha ) , IL 6 and tumor necrosis factor alpha ( TNF alpha ) production were measured from the stimulated monocytes and IL 2 , IL 4 and gamma interferon ( IFN gamma ) were measured from the stimulated T cells . ^^^ In contrast IL 2 , IL 4 , TNF alpha and IFN gamma production were not affected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The inhibitory effect was not reproduced by TGF beta , IFN gamma , IFN alpha , TNF alpha , LIF , SCF or Il 6 , and was not impaired by inhibitors of nitric oxide or PGE production in the LTMC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PGE 2 and tumor necrosis factor alpha ( TNF alpha ) , two factors produced by LPS stimulated M phi , were tested for their ability to modulate class 2 mRNA expression in NH and TBH IFN gamma treated M phi . ^^^ The addition of TNF alpha to IFN gamma treated M phi suppressed class 2 mRNA in NH M phi but , surprisingly , had an additive effect on IFN gamma induced class 2 mRNA expression . ^^^ TNF alpha did not induce class 2 mRNA expression in TBH M phi in the absence of IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In thioglycollate elicited macrophages from wild type mice , tumor necrosis factor ( TNF ) alone was virtually ineffective in inducing release of NO 2 ( the endproduct of nitric oxide generation ) , but TNF enhanced NO 2 release in the presence of IFN gamma . ^^^ In macrophages from IFN gamma R0 / 0 mice , which were unresponsive to IFN gamma , TNF completely failed to stimulate NO 2 release . ^^^ The stimulatory actions of IFN alpha / beta on NO 2 release were indistinguishable in wild type and IFN gamma R0 / 0 macrophages : IFN alpha / beta was ineffective on its own , showed marginal stimulation of NO 2 release in combination with TNF , and was moderately effective in the presence of lipopolysaccharide . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Such cytokines as interferon gamma ( IFN gamma ) , IL 3 or granulocyte macrophage colony stimulating factor ( GM CSF ) amplify the inflammatory response by increasing the production of IL 1 and TNF by macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
By using a sensitive reverse polymerase chain reaction technique , we observed that transcription of IL 5 , GM CSF , and TNF alpha could be detected in unstimulated , IL 2 starved TIL from all cultures , while IL 4 and IFN gamma genes were found to be transcribed in two cultures out of eight . ^^^ Despite detection of their gene transcripts , GM CSF , IFN gamma , or TNF alpha was never detectable , at the protein level , in unstimulated TIL . ^^^ In contrast , rhIL 2 induced IFN gamma and TNF alpha production in three and one TIL population , respectively . ^^^ Addition of rhIL 2 significantly increased production of GM CSF , but not of IFN gamma and TNF alpha . ^^^ On the other hand , triggering of the CD 3 T cell receptor complex induces transcription of an extended panel of cytokine genes in all TIL populations and secretion of GM CSF , IFN gamma and TNF alpha in virtually all cultures . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The synthesis of IFN gamma and TNF alpha was unaffected of the influence of i . v . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Renal cells which were previously stimulated by culture with either interferon gamma ( IFN gamma ) or supernatant from mixed leucocyte cultures ( MLC ) were relatively resistant to such lysis ; stimulation with tumour necrosis factor alpha ( TNF alpha ) had no effect . ^^^ The expression of class 1 major histocompatibility complex ( MHC ) antigens was up regulated by stimulation of renal cells with either IFN gamma or MLC supernatant ; treatment with TNF alpha had no effect on the expression of these antigens . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Only low proportions of R NK cells expressed genes for IL 2Rp55 ( 16 % ) or cytokines IL 2 ( 20 % ) , IFN gamma ( 18 % ) , TNF alpha ( 16 % ) , and TGF beta ( 7 % ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Studies with neutralizing antibodies indicated that both IFN gamma and IL 1 alpha / beta were required for the activity but that TNF alpha was not . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Monocytes labeled with 51Cr accumulated in delayed type hypersensitivity reactions , and sites injected with killed Escherichia coli , LPS , poly inosine : cytosine , zymosan activated serum ( ZAS ) , a source of C5adesArg , and the cytokines IL 1 alpha , TNF alpha , and IFN gamma . ^^^ Combinations of LPS , TNF alpha , and IFN gamma synergistically enhanced the late ( > 5 h ) but not the early phase of monocyte recruitment . ^^^ In conclusion , purified monocytes isolated from rat blood can be used to quantify monocyte migration in vivo , and these cells migrate rapidly to cutaneous inflammation and in response to chemotactic factors , IL 1 alpha , TNF alpha , and IFN gamma , with initial kinetics similar to those of neutrophils . ^^^ However , monocyte selective mechanisms are induced by IFN gamma and also appear to be involved in prolonged monocyte migration to TNF alpha , LPS , and E . coli . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In monocyte cultures , induction of IFN alpha by HIV 1 was relatively specific : Levels of IL 1 beta , IL 6 , IFN gamma , and TNF alpha remained at baseline . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To investigate the factors regulating the entry of lymphocytes into the brain , we assessed the adhesion in vitro of 51Cr labelled lymphocytes from peripheral blood of patients with multiple sclerosis ( MS ) to human cerebral endothelial cells , and evaluated the effect on the adhesion of endothelium activated by interferon gamma ( IFN gamma ) , lipopolysaccharides ( LPS ) , interleukin 1 ( IL 1 ) and tumor necrosis factor ( TNF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of IL 1 beta , TNF alpha and interferon gamma ( IFN gamma ) mRNA and their cytokine production were superior in NK cells compared with T cells in parallel with their LAK activities . ^^^ An increase of TNF alpha , IL 1 beta and IFN gamma production was induced by co culturing NK or T cells with autologous monocytes . ^^^ IFN gamma , which was produced at a later stage , scarcely affected LAK induction in spite of the cooperation with TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Blocking TNF alpha activity with anti TNF alpha antibodies caused IFN gamma to suppress alloreactivity in all M phi added cultures . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The best compromise between sensitivity and linearity was obtained with the addition of 50 ng / well for TNF alpha and IL 6 or 100 ng / well for IFN gamma of unconjugate antibodies to the corresponding conjugate . ^^^ The kinetics of individual production of each cytokine in WB of normal healthy donors showed values entering the standard range following incubation times of between 2 and 8 h for TNF alpha , 2 and 4 h for IL 6 , and 4 and 24 h for IFN gamma . ^^^ The sensitivity , the precision ( intra assay CVs ) and the reproducibility ( interassay CVs ) of the assays were as follows : 70 pg / ml , < or = 14 % and < or = 11 % for TNF alpha ; 25 pg / ml , < or = 11 % and < or = 16 % for IL 6 ; 25 pg / ml , < or = 19 % and < or = 20 % for IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunohistological staining with anti human CD 56 , CD 3 , TNF alpha , and IFN gamma antibodies demonstrated CD56+ cells in association with TNF alpha and IFN gamma secretion in the bowel of NK injected animals . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cell line was found to produce several cytokines : M CSF , IFN gamma and TNF alpha , as demonstrated by Western blotting analysis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We compared the abilities of gamma interferon ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and sensitized murine lymph node lymphocytes to activate syngeneic murine peritoneal macrophages to inhibit the growth of intracellular Mycobacterium bovis BCG in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Direct evidence for the role of T cells and cytokine activated macrophages in the host defense reaction against a primary Yersinia infection in mice could be obtained from experiments including adoptive transfer of Yersinia specific T cells and in vivo neutralization of TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We demonstrated that 1 ) the human eosinophilic leukemia cell line , EoL 1 , did not release H2O2 when stimulated with phorbol myristate acetate ( PMA ) , but after culturing with tumor necrosis factor ( TNF ) and interferon gamma ( IFN gamma ) it acquired the ability to release H2O2 ; 2 ) the ability to release H2O2 was time dependent and reached a peak after 4 days of culture ; 3 ) administration of TGF beta or GM CSF , with TNF and IFN gamma enhanced the PMA induced release of H2O2 from EoL 1 . ^^^ To examine the potential relationship between c myc gene expression and induction of the ability to release H2O2 , Northern analysis of c myc gene expression in EoL 1 cocultured with TNF and IFN gamma was performed . ^^^ The results showed that the c myc gene was spontaneously expressed in EoL 1 , and the level of c myc mRNA was markedly reduced after the cells were cocultured with TNF and IFN gamma , suggesting that the decrease of the c myc mRNA level is closely associated with induction of the ability to release H2O2 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment with CsA had no effect on the sensitivity L 929 cells to TNF mediated cytotoxicity , either in the presence or absence of interferon gamma ( IFN gamma ) . ^^^ In the presence of IFN gamma alone , L 929 cells were slightly less sensitive to the cytotoxic effects of TNF . ^^^ In contrast to the effect on TNF mediated cytotoxicity , CsA treatment had a profound effect on the ability of these cells to produce NO in response to TNF and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytolysis , TNF activity and NO 2 production were enhanced by lipopolysaccharide ( LPS ) and interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN alpha , IFN gamma TGF beta and IL 10 are inhibitory , whereas IL 5 , IL 6 and TNF alpha act synergistically with IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Addition of 1 to 50 U / ml IFN gamma induced a dose dependent increase in NO 2 production , with the maximal level approximating that found in suppressed cocultures ; TNF alpha , IL 2 , or LPS did not synergize with IFN gamma to enhance NO 2 production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor and interleukin ( IL ) 6 levels were not affected by anti IFN gamma treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The mechanism by which IL 4 and IL 10 acted to inhibit DTH responses did not appear to be through inhibition of IFN gamma or tumor necrosis factor production as treatment with antibodies which neutralized these activities failed to inhibit DTH responses . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma and TNF alpha secretion by CD4+ and CD8+ TCR alpha beta + T cell clones derived early after allogeneic bone marrow transplantation . ^^^ Secretion of the potentially antileukaemic cytokines IFN gamma and TNF alpha was investigated for CD4+ and CD8+ TCR alpha beta + T cell clones derived from 4 leukaemia patients 3 6 weeks after allogeneic BMT . ^^^ All clones derived after BMT were capable of IFN gamma and TNF alpha secretion , and both for CD4+ ( n = 96 ) and CD8+ ( n = 8 ) T cells quantities of IFN gamma and TNF alpha were significantly correlated with one another . ^^^ When comparing the overall results for posttransplant and normal T cell clones derived from 2 bone marrow donors ( n = 65 ) , both CD4+ and CD8+ TCR alpha beta + T cell clones showed increased IFN gamma production , and CD4+ but not CD8+ clones showed a decreased TNF alpha secretion . ^^^ The results suggest that noncytotoxic T cells derived after allogeneic BMT can produce IFN gamma and TNF alpha and may thus be capable of mediating antileukaemic effects . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It is suggested that the exacerbated in vivo response to PPD in lepromatous leprosy is the result of an increased immunoreactivity to the antigen , which well may be associated with the local and / or systemic release of cytokines [ tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) ] by the inflammatory cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the CD8+ subset , IFN gamma and HIV 1 mutually enhanced the production of TNF alpha , leading to hyperactivation of viral replication , whereas in CD 8 NK cells IFN gamma primed HIV induced IFN alpha production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and IL 6 were found to be produced in significant amounts by marrow mononuclear cells during culture in IL 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
As for TNF alpha and IFN gamma , no particular changing pattern was observed after transarterial administration . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The percentages of activated monocytes / macrophages , T cells , and possibly B cells ; of gamma / delta T cell receptor ( TCR ) bearing T cells ; of CD 4 CD 8 alpha / beta TCR bearing T cells ; and serum levels of tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and interleukin 6 ( IL 6 ) were high in patients with active visceral leishmaniasis . ^^^ TNF alpha , IL 6 , and IFN gamma may prove to be useful markers for monitoring response to therapy . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Reduced lethality of LPS correlated with a drastically reduced production of tumor necrosis factor alpha ( TNF alpha ) in the IFN gamma R0 / 0 mice . ^^^ The greatly reduced capacity of BCG infected IFN gamma R0 / 0 mice to produce TNF alpha may be an important factor in their inability to resist BCG infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
No correlation was found between the effect of BCG on the development of lesions induced by L . major and the amounts of IFN gamma , IL 5 and TNF produced after in vitro antigenic challenge of either draining lymph node or spleen cells , the antigenic challenge being either live BCG or live L . major . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To examine the possibility that cytokines produced in inflamed joint tissues may contribute to the loss of articular cartilage by causing inhibition of synthesis of cartilage specific matrix macromolecules , we studied the effects of interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) , alone and in combination , on the expression of the genes for types 2 , 9 and 11 collagens in cultured human chondrocytes . ^^^ Chondrocytes isolated from human fetal epiphyseal cartilage by sequential enzymic digestions were cultured in the presence of IFN gamma ( 30 pM ) , TNF alpha ( 15 pM ) or a combination of suboptimal concentrations of both cytokines ( 1 . 5 pM IFN gamma plus 0 . 3 pM TNF alpha ) . ^^^ TNF alpha was a more potent inhibitor causing a 42 . 8 45 . 3 % decrease at one half the concentration of IFN gamma . ^^^ A synergistic inhibitory effect of 58 . 2 % was observed with the combination of 1 . 5 pM IFN gamma plus 0 . 3 pM TNF alpha . ^^^ These observations indicate that IFN gamma and TNF alpha can induce a synergistic inhibition of the synthesis of cartilage specific collagens by fetal and adult human chondrocytes and suggest that these effects may contribute to the articular cartilage loss that occurs in inflammatory joint diseases . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Salmonella typhimurium SH 5014 porins induce the release of tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 alpha ( IL 1 alpha ) , and IL 6 by human monocytes and of gamma interferon ( IFN gamma ) and IL 4 by human lymphocytes . ^^^ Porins at 1 microgram / ml induce the greatest release of TNF alpha , IL 1 alpha , and IL 6 by monocytes and of IL 4 by lymphocytes , while porins at 5 micrograms / ml induce the greatest release of IFN gamma by lymphocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A noncytolytic , Ag specific clone produced IL 6 , tumor necrosis factor ( TNF ) , and lymphotoxin ( LT ) , but no IL 2 , IL 4 , or IFN gamma mRNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha , IFN gamma , and aggregates of IgG increased the synthesis of CSF 1 ( determined by RIA ) and of MCP 1 ( determined by biolabeling and immunoprecipitation ) . ^^^ Stimulation of cAMP generation by forskolin or PGE 2 decreased basal CSF 1 synthesis and attenuated the responses to TNF alpha , IFN gamma , and IgG . ^^^ Forskolin and PGE 2 also decreased biolabeled MCP 1 generation after stimulation with IFN gamma , TNF alpha , or IgG . ^^^ By Northern blot analysis steady state levels of mRNA for CSF 1 and JE / MCP 1 were increased after incubation with IFN gamma , TNF alpha , or IgG , and these effects were attenuated by forskolin . ^^^ Thus , agents stimulating cAMP generation , including PGE 2 , counterbalance the generation of CSF 1 and JE / MCP 1 in response to IFN gamma , TNF alpha , and IgG complexes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
M 40 cells were also resistant to growth inhibition by tumor necrosis factor alpha ( TNF alpha ) and interleukin 1 alpha ( IL 1 alpha ) but were inhibited by interferon gamma ( IFN gamma ) and heparin . ^^^ These results imply that TGF beta 1 , TNF alpha , and IL 1 alpha act through signal transducing pathways that are separate from pathways for IFN gamma and heparin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this report we demonstrate that the cytokine combination of human recombinant interleukin 1 beta ( IL 1 beta ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) induces the formation of nitric oxide by human islets . ^^^ IL 1 beta and IFN gamma are sufficient to induce nitric oxide formation by human islets , whereas TNF alpha potentiates nitrite production . ^^^ This combination of cytokines ( IL 1 beta , TNF alpha , and IFN gamma ) also influences insulin secretion by human islets . ^^^ Pretreatment of human islets with low concentrations of this cytokine combination ( IL 1 beta at 15 units / ml , 0 . 7 nM TNF alpha , and IFN gamma at 150 units / ml ) appears to slightly stimulate insulin secretion . ^^^ Higher concentrations ( IL 1 beta at 75 units / ml , 3 . 5 nM TNF alpha , and IFN gamma at 750 units / ml ) inhibit insulin secretion from human islets , and the inhibitory effect is prevented by NG monomethyl L arginine . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Of these mediators LTB 4 , ZAP , IL 1 alpha , TNF alpha , IFN gamma , and IL 8 have been reported to induce neutrophil accumulation in skin of sheep , and in the current study E . coli endotoxin was a potent inducer of 111In labeled neutrophil accumulation and plasma leakage in skin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although the level of interferon gamma ( IFN gamma ) in TBPE measured by ELISA was significantly higher than in non TBPE , there was no significant difference in the levels of tumour necrosis factor alpha ( TNF alpha ) measured by ELISA between these two groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Isolated rat hepatocytes were examined by EPR spectroscopy after exposure to inflammatory stimuli ( interferon gamma [ IFN gamma ] , tumor necrosis factor alpha [ TNF alpha ] , interleukin 1 beta [ IL 1 beta ] , and lipopolysaccharide [ LPS ] ) in vitro , after in vivo immune activation by Corynebacterium parvum , and after exposure to . ^^^ Hepatocytes exposed to IFN gamma , TNF alpha , IL 1 beta , and LPS demonstrated the appearance of a g = 2 . 04 axial EPR signal indicative of the formation of nonheme iron nitrosyl complexes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study we have investigated the effects of two cytokines , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) , on the regulation of IGF 2 gene expression in cultured HFA cells . ^^^ Both TNF alpha and IFN gamma inhibited basal and ACTH induced accumulation of IGF 2 mRNA dose dependently . ^^^ Cell viability was not altered by treatment with TNF alpha or IFN gamma . ^^^ In addition , the combination of TNF alpha and IFN gamma decreased ACTH induced IGF 2 mRNAs more potently than each cytokine alone . ^^^ Our results suggest that TNF alpha and IFN gamma may be involved in the regulation of HFA growth and differentiation via local IGF 2 production . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study investigated the expression of HLA class 1 antigens on Huh 6 and HB 611 cells induced by interferon ( IFN ) alpha , IFN gamma , tumor necrosis factor ( TNF ) alpha and interleukin ( IL ) 1 beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) also had an inhibitory effect for KY 821 and KY MTX , but lacked such effect in KY RA and KY VCR . nIL 1 , IFN gamma and TNF alpha could not differentiate between any of the cell lines but IFN gamma and TNF alpha induced monocytic surface antigens . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effect of different doses of BCH 189 and AZT was analysed in vitro on : ( 1 ) T cell proliferation after stimulation with concanavalin A ( Con A ) or anti CD 3 MoAb ; ( 2 ) B cell proliferation and immunoglobulin production after stimulation with pokeweed mitogen ( PWM ) ; ( 3 ) cytokine production ( IL 2 , IL 6 , GM CSF , tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) from lymphocytes stimulated with anti CD 3 MoAb or phytohaemagglutinin ( PHA ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mutual antagonism between interferon gamma and tumor necrosis factor alpha on fibroblast like synoviocytes : paradoxical induction of IFN gamma and TNF alpha receptor expression . ^^^ We recently described mutual antagonism between IFN gamma and TNF alpha on human fibroblast like synoviocytes ( FLS ) . ^^^ TNF alpha inhibits IFN gamma induced HLA DR expression and IFN gamma blocks TNF alpha dependent synoviocyte proliferation , collagenase production , and GM CSF secretion . ^^^ TNF alpha did not competitively inhibit 125I IFN gamma binding . ^^^ When FLS were incubated with TNF alpha ( 100 ng / ml ) , there was a paradoxical 49 . 5 + / 5 . 6 % increase in the number of binding sites for IFN gamma ( P = 0 . 001 ) , with no change in the Kd . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Since human TNF has retained antitumor activity , esp . in combination with IFN gamma , TNF mutants that are selective agonists for the TNF R 55 might have a broader therapeutic margin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pretreatment with IL 6 induced a dose and time dependent suppression of IFN gamma ( 1000 U / mL ) and TNF alpha ( 25 ng / mL ) activation of M phi for the killing of L . amazonensis . ^^^ At doses greater than 0 . 1 to 100 ng / mL , IL 6 inhibited IFN gamma and TNF alpha activation by 21 to 93 % and 36 to 82 % , respectively . ^^^ Blockade of M phi activation was greatest when IL 6 was added 24 or 48 h before infection and treatment with IFN gamma or TNF alpha . ^^^ Furthermore , NG monomethyl L arginine ( a nitric oxide synthase inhibitor ) had no effect on IFN gamma and TNF alpha activation of antileishmanial activity and nitrites / nitrates were not reliably assayed from M phi culture supernatants . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Constitutive synthesis of cytokines was evaluated by analysis of both the expression of the mRNA for interleukin ( IL ) 1 beta , IL 4 , IL 8 , granulocyte / macrophage colony stimulating factor ( GM CSF ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) , by the polymerase chain reaction ( PCR ) , and by immunocytochemical staining for the presence of cell associated IL 1 beta , IL 8 , GM CSF , TNF alpha , and IFN gamma , using specific monoclonal and polyclonal antibodies directed towards these cytokines . ^^^ Release of IL 4 , IL 8 , GM CSF , TNF alpha , and IFN gamma following exposure to 5 % CO 2 in air or 400 ppb and 800 ppb NO 2 for 6 h was investigated by enzyme linked immunosorbent assay . ^^^ PCR demonstrated that the human bronchial epithelial cells expressed the mRNA for IL 1 beta , IL 8 , GM CSF , and TNF alpha but not for IL 4 and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To determine if mononuclear cells ( MNC ) infiltrating various types of human solid tumours express genes for cytokines , in situ hybridisation with 35S labelled cDNA antisense probes for interleukin 2 ( IL 2 ) , interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) , interleukin 1 beta ( IL 1 beta ) , transforming growth factor beta ( TGF beta ) and interleukin 2 receptors ( IL2R ) was performed . ^^^ In contrast , breast carcinomas producing mucin and all SCCHN contained numerous MNC expressing gene transcripts for IL 2 , IFN gamma , TNF alpha , IL2R as well as TGF beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , we noted an increase in the cytokine and growth factor receptors TNF R , IL 1R type 2 , IL 2R , IFN gamma R and PDGF beta R . ^^^ In contrast , in mesangial IgA GN , IL 1 beta , TNF alpha , IFN gamma and IL 2 were usually absent in glomeruli . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A quantity ten times more of S . epidermidis than of S . aureus cells was required to induce similar levels of TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Analysis of the fluid phase of SCs from positive DTH reactions disclosed high levels of TNF and IFN gamma ; neither was detected from SC ' s covering sites injected with nonspecific antigens . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Finally , the IFN gamma induced enhancement of antibrucella activities by peritoneal macrophages was inhibited by addition of antitumor necrosis factor alpha ( TNF alpha ) antibodies to the cultures , indicating that TNF alpha is necessary for full expression of the macrophage antibrucella activities . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This pattern of reactivity is different from the responsiveness of the MOLT 16 cells , a TcR alpha beta bearing cell line , which respond to IFN alpha and TNF alpha , but not to IFN gamma , by augmentation of IL 2 production . ^^^ These results suggest that ( 1 ) the addition of IFN gamma has a direct effect on PHA stimulated MOLT 13 and MOLT 14 , resulting in an augmentation of IL 2 production ; ( 2 ) phenotypically different T cell lines vary in their susceptibility to IFN alpha and IFN gamma regulation ; and ( 3 ) the augmentation of IL 2 production by TNF alpha appears to have no correlation with phenotypically different T cell clones . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment triggered a sustained increase in serum levels of IL 1 , IL 6 , TNF alpha and IFN gamma in all patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Irradiated T cells showed a reduced ability to proliferate , irrespective of the stimulus used , and a reduced ability to produce cytokines IL 2 , IL 4 , IL 5 , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , activin A neither induced nor augmented the production of TNF beta or interferon gamma ( IFN gamma ) , both of which are known to be exclusively generated by T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Also , in vitro activation of peritoneal macrophages with interferon gamma ( IFN gamma ) , increased TNF response to several stimuli . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Scatchard binding analysis has been employed to characterize expression of low affinity receptors for tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) and granulocyte macrophage colony stimulating factor ( GM CSF ) by isolated human placental syncytiotrophoblast microvillous plasma membrane ( StMPM ) vesicles . ^^^ No high affinity receptors could be detected for GM CSF or IFN gamma , but a minority of high affinity TNF alpha receptors were identified . ^^^ Only relatively high affinity receptors for TNF alpha and IFN gamma could be detected on the BeWo human choriocarcinoma cytotrophoblast cell line , whereas receptor affinity for GM CSF was similar to that on syncytiotrophoblast . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
On days 14 and 18 , levels of these transcripts had increased and transcripts for IL 6 , IL 10 , gamma interferon ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and granulocyte macrophage colony stimulating factor ( GM CSF ) were also detected . ^^^ To assess the possible role of endogenous IFN gamma , TNF alpha , IL 10 , IL 6 , and GM CSF , cytokine neutralizing monoclonal antibodies were administered to infected SCID mice . ^^^ Neutralization of IFN gamma or TNF alpha led to earlier mortality than that in controls . ^^^ These results indicate that TNF alpha and IFN gamma are both involved in T cell independent mechanisms of resistance to T . gondii in SCID mice and that IL 10 and IL 6 may downregulate the immune response to this pathogen . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
LAMH37Ra , like gram negative LPS , exhibited a dose dependent ability to induce tumor necrosis factor alpha ( TNF alpha ) production in normal M phi , and gamma interferon ( IFN gamma ) priming of the M phi greatly augmented the levels of TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Supernatants from the spleen cells cultured in vitro for 24 or 48 h in medium alone or with CneF , concanavalin A , or phorbol 12 myristate 13 acetate plus calcium ionophore were assessed for the presence of interleukin 2 ( IL 2 ) , gamma interferon ( IFN gamma ) , IL 4 , IL 5 , and tumor necrosis factor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokines evaluated included interleukin 1 alpha ( IL 1 alpha ) , IL 1 beta , IL 2 , IL 3 , IL 4 , IL 5 , IL 6 , IL 10 interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , TNF beta , and granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Northern blot analysis showed that IL 1 alpha and IFN gamma strongly increase the levels of mRNA that encodes for MCP 1 , whereas TNF alpha appears to be a weaker stimulus . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , C syntheses in this line were independently upregulated by several human cytokines : C 3 synthesis was dose dependently enhanced by the addition of IL 1 beta or TNF alpha ; C 4 synthesis was enhanced by the addition of IL 6 or IFN gamma in the same manner ; and the addition of IL 1 beta or IL 6 also induced a dose dependent increase in factor B synthesis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines examined included IL 1 alpha , IL 2 , IL 3 , IL 4 , IL 6 , IL 7 , IL 8 , IL 10 , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , granulocyte / macrophage colony stimulating factor ( GM CSF ) , and macrophage inflammatory protein 1 alpha ( MIP 1 alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast to results reported previously with both resident and elicited PMs from mice , tumor necrosis factor alpha ( TNF alpha ) exerted only a small enhancing effect on IFN gamma induced nitric oxide production by resident rat PMs . ^^^ In contrast to PMs , AMs had a strong synergistic response to combinations of IFN gamma and LPS or TNF alpha but also only at relatively low concentrations of IFN gamma and LPS . ^^^ Furthermore , maximum nitric oxide production induced by IFN gamma in these cells could be enhanced by TNF alpha or low doses of LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When AM were primed by IFN gamma ( 500 units / ml ) and triggered by LPS ( 100 micrograms / ml ) , TNF alpha production by AM was significantly smaller in old mice as compared with young mice ( young mice : 161 . 7 + / 28 . 2 units / ml ; old mice : 89 . 3 + / 13 . 6 units / ml , P < 0 . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Thus , IFN gamma and TNF alpha were both critical for resolution of infection with L . major , with IFN gamma ' s role limited to the first 2 days . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
As part of continuing studies to investigate the possible regulatory effects of cytokines on malignant astrocytes , we investigated the effects of interleukin 4 ( IL 4 ) alone and in combination with tumor necrosis factor alpha ( TNF alpha ) and / or interferon gamma ( IFN gamma ) on the cell growth and major histocompatibility complex ( MHC ) antigen expression of a cloned human glioblastoma cell line ( 9C ) . ^^^ The 9C cells were treated with IL 4 alone or in combination with TNF alpha and / or IFN gamma and were examined for proliferation by crystal violet assay and for Class 2 MHC antigen by flow cytometry . ^^^ In combination with TNF alpha or IFN gamma , however , IL 4 significantly and dose dependently inhibited cell growth . ^^^ As previous reports have shown , TNF alpha combined with IFN gamma exerted an additive growth suppressive effect on glioblastoma cells , probably by enhancing TNF receptor expression . ^^^ This additive effect of TNF alpha and IFN gamma was further enhanced by IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin 1 alpha ( IL 1 alpha ) , IL 2 , IL 4 , IL 6 , gamma interferon ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and granulocyte macrophage colony stimulating factor ( GM CSF ) were tested for their abilities to alter the growth of Brucella abortus in BALB / c J774A . 1 murine macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the CD 4 T cells , on a per cell basis , the mean mRNA levels of IFN gamma , IL 10 , and TNF alpha were increased substantially ( p < 0 . 001 ) in HIV infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have measured TNF alpha , interleukin 1 alpha ( IL 1 alpha ) , interleukin 6 ( IL 6 ) , and gamma interferon ( IFN gamma ) levels in neonatal rats infected with different strains ( H 738 , 259 , and 90 ) and doses ( 1 50 % lethal dose [ LD 50 ] and 5 90 % lethal doses [ LD 90 ] ) of type 3 GBS . ^^^ Plasma TNF alpha bioactivity was undetectable in anti TNF alpha treated animals , while IL 6 and IFN gamma , but not IL 1 alpha , levels were significantly reduced , compared with normal serum controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , gamma interferon ( IFN gamma ) was never detected in mice infected with 10 minimum lethal doses ( MLD ) of Lcr+ cells of Y . pestis , and significant levels of tumor necrosis factor alpha ( TNF alpha ) arose only prior to death . ^^^ Treatment of mice with exogenous IFN gamma plus TNF alpha inhibited multiplication of Lcr+ yersiniae in vivo , thereby providing protection against challenge with 10 MLD . ^^^ This protective effect entailed cytokine priming as judged by subsequent detection of substantial levels of endogenous IFN gamma and TNF alpha . ^^^ Monospecific anti 5 antigen , known to provide passive immunity against 10 MLD of Lcr+ Y . pestis , permitted significant synthesis of endogenous IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Since it is known that interferon gamma ( IFN gamma ) may induce the production of TNF alpha , the secretion of this cytokine was examined . ^^^ Addition of IFN gamma to LpMNC did not result in enhanced TNF alpha secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of IFN gamma and TNF alpha mRNA in normal and LPS treated mice was examined by Northern blot analysis and amplification using the polymerase chain reaction . ^^^ Messenger RNA for IFN gamma and TNF alpha could not be reliably detected by Northern analysis in spleens or kidneys of normal mice . ^^^ After treatment with cycloheximide , a protein synthesis inhibitor , IFN gamma and TNF alpha mRNA could be detected in both sites in otherwise normal mice . ^^^ The level of both IFN gamma and TNF alpha mRNA increased after LPS , although the temporal patterns of expression were different . ^^^ Cyclosporin A blocked induction of IFN gamma in a dose dependent manner , but failed to significantly inhibit TNF alpha mRNA or protein expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
At disease onset , patients with active tuberculosis were characterized by ( 1 ) T cell unresponsiveness to most A 60 fractions , ( 2 ) high tumor necrosis factor alpha production , and ( 3 ) low gamma interferon ( IFN gamma ) release . ^^^ Several weeks after chemotherapy , the unresponsive state disappeared and the following reverse situation was observed : ( 1 ) high blastogenic response to almost all A 60 fractions , ( 2 ) low tumor necrosis factor alpha release , and ( 3 ) high IFN gamma production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
McCoy cells , murine derived cells commonly used for propagation of chlamydiae , were found to be efficient producers of nitric oxide ( NO ) when primed with murine gamma interferon ( IFN gamma ) and then exposed to the second signals provided by Escherichia coli lipopolysaccharide , human interleukin 1 alpha , murine tumor necrosis factor alpha , or Chlamydia trachomatis type H . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both NO 2 production and inhibition of bacterial growth were suppressed by NG monomethyl L arginine , a substrate inhibitor of nitrogen oxidation of L arginine , and monoclonal antibodies ( MAbs ) to gamma interferon ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^ Treatment of these BCG infected mice with MAbs to IFN gamma or TNF alpha at the time of BCG inoculation reduced urinary NO 3 levels to those found in normal uninfected mice for up to 14 days . ^^^ Intraperitoneal administration of anti IFN gamma or anti TNF alpha antibody 8 days after BCG infection also reduced urinary NO 3 and abolished protection against F . tularensis . ^^^ IFN gamma and TNF alpha were clearly involved in the regulation of macrophage activation by BCG in vivo . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Other cytokines also known to influence the proliferation , differentiation , or recirculation of B lymphocytes such as IFN gamma , IL 2 , IL 4 , IL 6 , TNF alpha , and low molecular weight B cell growth factor did not induce either Leu 13 expression or homotypic aggregation of Daudi B cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , TNF alpha and IFN gamma in combination exerted a synergistic effect on both MCP 1 mRNA accumulation and protein secretion . ^^^ These studies demonstrate that the MCP 1 gene is regulated by TNF alpha and IFN gamma in type B synoviocytes and indicate that these cells may play an important role in the recruitment of inflammatory cells to the rheumatoid synovial environment , via the production of novel chemotactic cytokines such as MCP 1 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Recombinant human tumor necrosis factor and recombinant human gamma interferon ( IFN gamma ) exert synergistic growth inhibitory effects in WiDR human colorectal carcinoma cells . ^^^ In this cell line , tumor necrosis factor increases IFN gamma binding . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Thus , IFN gamma has proinflammatory effects through the combination of two mechanisms : ( a ) induction of early tumor necrosis factor alpha ( TNF alpha ) and IL 1 beta synthesis ; and ( b ) inhibition of the delayed production of IL 10 , an inhibitor of TNF alpha and IL 1 beta synthesis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Purified malignant and non malignant B lymphocytes were incubated with interleukin 4 ( IL 4 ) , interferon alpha ( IFN alpha ) and IFN gamma , and the supernatants were tested for the production of TNF alpha using an enzyme linked immunosorbent assay ( ELISA ) . ^^^ Hairy cell leukemia ( HCL ) cells produced TNF alpha in the absence of stimuli and this production was markedly enhanced by addition of IFN alpha or , to a lesser extent , by IFN gamma and IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma , but not tumor necrosis factor alpha , could be shown to be secreted by activated DLN cells in vitro in response to specific tumor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To examine the role of this mechanism in the killing of Mycobacterium avium by murine and human macrophages , we infected mouse peritoneal macrophages and human monocyte derived macrophages with M . avium and Listeria monocytogenes and stimulated the cells with recombinant tumour necrosis factor ( TNF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) or IFN gamma , in the presence or absence of N monomethyl L arginine ( NMA ) or arginase . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We determined the effects on endothelial monolayer permeability of acute agonists ( thrombin and histamine ) , cytokines ( tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , IL 1 and IL 4 ) and combinations of acute agonists and cytokines . ^^^ For other agonists and cytokines the increases in permeability were : ( 1 ) histamine ( 50 400 pmol / ml ) increased uptake 5 22 % ; ( 2 ) TNF ( 12 . 5 100 ng / ml ) increased uptake 2 12 % ; ( 3 ) IFN gamma ( 125 250 U / ml ) increased uptake 1 . 5 3 % . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin 4 ( IL 4 ) down regulated TNF alpha production by monocytes , but in contrast interferon gamma ( IFN gamma ) moderately enhanced TNF alpha production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study examines quantitative changes in the level of tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) production in BAL cell cultures derived from patients ( n = 11 ) with bronchial asthma . ^^^ The secretion of TNF alpha and IFN gamma was determined in intact ( unstimulated ) and phytohemagglutinin / phorbol myristate acetate ( PHA + PMA ) stimulated BAL leukocyte cultures and compared with that in control cultures . ^^^ In all patients studied , the background and PHA + PMA induced secretion of TNF alpha and IFN gamma was significantly ( p < 0 . 001 ) higher than that in parallel control cultures . ^^^ In contrast to BAL cell preparations , the capacity of TNF alpha and IFN gamma secretion by patients ' peripheral blood mononuclear cells ( PBMC ) did not differ from that of control subjects . ^^^ High spontaneous release of TNF alpha and IFN gamma by patients ' BAL leukocytes , but not PBMC , suggest that in the pathophysiology of bronchial asthma , these cytokines may act as local pathogenic agents in the lung . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The ability of cytokines ( IL 4 , IL 5 , IL 6 , IFN alpha , IFN gamma , TNF alpha , GmCSF ) to regulate peak benzylpenicilloyl ( BPO ) specific IgE antibody forming cell ( AFC ) responses was investigated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Exposure to IL 1 resulted in enhanced 10 . 1 . 1 reactivity of this cell line , with little , if any , additional response to TNF alpha or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation of human blood monocytes ( adherent mononuclear cells ) and the monocytic cell line , THP 1 , by IL 1 or TNF alpha leads to the up regulation of IFN gamma receptors . ^^^ Both IL 1 and TNF alpha enhanced the IFN gamma induced HLA DR expression ( > twofold ) and this effect was inhibited by antibody to IFN gamma . ^^^ In the case of human monocytes , IL 1 or TNF alpha , each by themselves also increased HLA DR expression , which was also abrogated by antibody to IFN gamma . ^^^ The data suggest that the immunopotentiating effects of IL 1 and TNF alpha are mediated in part by enhancing IFN gamma receptor expression on monocytes and macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increasing amounts of IFN gamma ( to 100 units / ml ) elicited increasing levels of IP 10 messenger RNA ( mRNA ) , sustained to 24 hours , but had no effect on tumor necrosis factor alpha ( TNF alpha ) mRNA . ^^^ TNF alpha at doses up to 10 ng / ml and soluble immune complexes up to 150 micrograms / ml antibody evoked 3 to 5 fold increases in IP 10 mRNA expression , much less than the 30 to 70 fold increases seen with IFN gamma and LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Among five of six melanoma TIL for which autologous tumor was available , autologous melanoma cells stimulated specific release of at least one of three cytokines : GM CSF , IFN gamma , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Addition of 25 U / ml IFN gamma enhanced TNF alpha secretion in response to H37Ra LAM , reducing the threshold level of LAM required to 10 to 100 ng / ml . ^^^ In contrast , Erdman LAM at concentrations up to 1000 ng / ml could not induce macrophage TNF alpha secretion even in the presence of 25 U / ml IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Recent studies have shown that certain lymphokines such as tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) can significantly enhance O 2 production by phagocytic cells . ^^^ To ascertain lymphokine production in FA patients , we measured TNF alpha and IFN gamma production in vivo and in vitro . ^^^ Spontaneous and phytohemagglutinin ( PHA ) induced production of IFN gamma and TNF alpha by cultured peripheral blood mononuclear cells did not differ significantly between FA patients and normal controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Only a minority of cells expressed mRNA for TNF , IL 1 beta , IL 6 and IFN gamma , and monocytes were the main source . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Generally , arabinogalactan pretreatment induced an increased release of interferon gamma ( IFN gamma ) , tumor necrosis factor alpha , interleukin 1 beta ( IL 1 beta ) and IL 6 but only IFN gamma was involved in enhancement of NK cytotoxicity since cytotoxicity enhancement of PBMC and PNAC but not that of monocytes could be blocked when anti IFN gamma antibodies were present during pretreatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In view of cellular adoptive immunotherapy , we have studied the role of tumor necrosis factor alpha ( TNF alpha ) and gamma interferon ( IFN gamma ) in monocyte mediated cytotoxicity on the level of both effector and leukemic target cells . ^^^ Upon IFN gamma activation these monocytes showed a 20 fold increase in TNF alpha secretion of 663 + / 122 pg / mL . ^^^ Comparable concentrations of recombinant human TNF alpha showed only cytostatic effects on U 937 cells of approximately 20 % after 24 hours , similar to the cytostatic effects of IFN gamma activated monocyte culture supernatants . ^^^ IFN gamma activated monocytes were able to lyse TNF alpha modified U 937 cells whereas IFN gamma activated monocyte supernatants showed only cytostatic activity after prolonged incubation . ^^^ Additionally , target cell modulation by IFN gamma potentiated the TNF alpha dependent cytolytic and cytostatic effects of monocytes , monocyte culture supernatants and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The main goal of this study was to compare , using unstimulated and IFN gamma , TNF alpha or LPS pretreated EC , the adherence of T cells isolated from blood ( BTC ) , with the adherence of T lymphocytes obtained from other tissues . ^^^ However , their adherence was increased about 3 fold , when EC were pretreated with IFN gamma , TNF alpha or LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although various roles have been suggested for interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) and interleukin 1 alpha ( IL 1 alpha ) in GO , their actual presence in Graves ' retro ocular connective tissue has not been demonstrated . ^^^ We examined surgical specimens obtained during orbital decompression from patients with severe GO ( n = 6 ) , and from normal individuals ( n = 5 ) , for the presence of IFN gamma , TNF alpha and IL 1 alpha . ^^^ We detected immunoreactivity for the three cytokines ( IFN gamma , TNF alpha and IL 1 alpha ) in the five GO tissue specimens that contained mononuclear cell aggregates . ^^^ The presence of mononuclear cell infiltrates and associated immunoreactivity for IFN gamma , TNF alpha , IL 1 alpha in retro ocular connective tissue derived from patients with GO suggests that the previously demonstrated in vitro functions of these cytokines may indeed be operative in vivo . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated serum levels of interleukin 6 ( IL 6 ) , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) from patients with systemic lupus erythematosus ( SLE ) and its various clinical manifestations of disease and from patients with rheumatoid arthritis ( RA ) and other rheumatic diseases . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The TGIF activity detected in CSN of OK MC was further characterized as derived from a cytokine different from interferon gamma ( IFN gamma ) , tumor necrosis factor ( TNF ) , or lymphotoxin ( LT ) by a neutralization test using monoclonal antibodies to these cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Macrophage mediated inhibition of mitochondrial respiration in EMT 6 murine mammary adenocarcinoma cells can be mimicked in vitro by treatment of the cells with interferon gamma ( IFN gamma ) in combination with tumor necrosis factor , interleukin 1 , or lipopolysaccharide . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment with LPS and IFN gamma also induced significant production of prostaglandin E 2 ( PGE 2 ) , thromboxane B 2 ( TBX 2 ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 ( IL 1 ) , and IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
On measuring cytokine serum levels ( neopterin , IL 1 beta , IL 6 , sIL 2R , GM CSF , TNF alpha , IFN gamma ) we noticed a different cytokine pattern with both treatment modalities , resulting in a moderate induction of TNF alpha and IFN gamma during low dose , intravenous treatment , whereas during subcutaneous , high dose treatment a profound increase of IL 6 , sIL 2R , and , to a lesser extent , neopterin was detected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In particular , IL 1 beta and neopterin production were further enhanced by stimulation with either interferon gamma ( IFN gamma ) or TNF alpha without a synergistic effect . ^^^ The number of the receptors for IFN gamma and TNF alpha was not changed between first and third leukapheresis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Maximal induction of CEA by IFN gamma and tumor necrosis factor alpha ( TNF alpha ) in the colon carcinoma cell line HT 29 occurs at 5 6 days with maximal secreted levels at 14 ng / ml for IFN gamma and 20 ng / ml for TNF alpha . ^^^ Cell viability was reduced to 67 % of controls for TNF alpha and to 36 % for IFN gamma . ^^^ Dose response curves showed maximal induction of CEA at 500 units / ml for TNF alpha and at 200 units / ml for IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Simultaneous treatment of D 54 MG cells with IL 1 beta and interferon gamma ( IFN gamma ) resulted in an additive increase in both C 3 mRNA and protein expression , a finding not seen with the combination of TNF alpha and IFN gamma . ^^^ Primary rat astrocytes also express increased C 3 mRNA levels after 48 h in response to IL 1 beta ( 5 . 3 fold increase ) and TNF alpha ( 7 fold increase ) , while an additive effect was observed upon simultaneous treatment with both IL 1 beta and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the presence of IFN gamma , pep M 5 induced the release of TNF alpha , IL 1 , and IL 6 , TNF alpha levels peaked at 24 h , while IL 1 and IL 6 levels peaked at 48 h . pep M 5 induced T cells to produce IFN gamma , which may have accounted for the ability of the super antigen to induce the production of IL 1 , IL 6 , TNF alpha , and TNF beta by peripheral blood mononuclear cells ( PBMC ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Some cytokines ( IL 1 and IL 6 ) promote , whereas others ( IFN gamma and TNF ) inhibit intracellular M . avium growth . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Any of the cytokines considered as macrophage activating factor , such as IFN gamma , IL 2 , IL 4 , GM CSF , or TNF alpha , did not induce MGC by itself . ^^^ Furthermore , the enhancing effect of IFN gamma on this response was entirely abrogated by anti TNF alpha rabbit antibody . ^^^ Our results indicate that T cell derived lymphokines , such as IFN gamma and IL 4 , have mutually antagonistic effects on Con A induced human MGC formations in vitro , in which the expression of TNF alpha in human monocytes plays a key role in the fusion process of monocytes . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This CD 3 down regulating activity in fractionated supernatant was not inhibited by any lymphokine antibodies , anti IL 1 alpha antibody ( Ab ) , anti IL 1B Ab , anti IL 2 Ab , anti IL 3 Ab , anti IL 4 Ab , anti IL 6 Ab , anti TNF alpha Ab and anti IFN gamma Ab . ^^^ Any known cytokines ( IL 1 , IL 2 , IL 3 , IL 4 , IL 6 , TNF alpha and IFN gamma ) could not modulate CD 3 expression of normal PBMC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The alloreactive NK clones produced cytokines with a suppressive effect on in vitro hematopoiesis , such as interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) , when exposed to phytohemagglutinin blasts from specificity 1 susceptible , but not resistant donors . ^^^ However , the mechanism by which alloreactive NK cells inhibit colony formation is more consistent with a direct cytotoxic effect than with the production of inhibitory cytokines because antibodies ( anti IFN gamma , alpha TNF alpha , and lymphotoxin ) that completely blocked the inhibition by polyclonal NK cells had only a minimal effect on the inhibition by the alloreactive clones . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In order to investigate the relationships between cytokine production and arthritic disease we have determined the concentrations of immunoreactive interleukin 1 alpha ( IL 1 alpha ) , IL 1 beta , IL 6 , tumour necrosis factor alpha ( TNF alpha ) , interferon alpha ( IFN alpha ) , IFN gamma , and soluble IL 2 receptor ( sIL 2R ) , as well as bioactive IL 1 and IL 6 , in synovial fluids ( SF ) and plasma of patients with a variety of arthritides . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin ( IL ) 2 , IL 4 , IL 7 , tumor necrosis factor ( TNF ) , or interferon gamma ( IFN gamma ) has been shown to be able to induce tumor rejection if produced locally by the tumor cells after gene transfer . ^^^ The transient tumor suppression induced by IL 2 , IL 4 , TNF , or IFN gamma was also operative in nude and severe combined immunodeficient mice , whereas only tumor suppression induced by IL 7 was dependent on the presence of CD4+ T cells and was not evident in nude mice . ^^^ The T cell independent effector arm of IL 2 and IFN gamma but not IL 4 and TNF was mediated in part by natural killer cells . ^^^ The transience of tumor suppression in the absence of T cells reflected loss of cytokine production in the case of TNF , IL 2 , and IL 4 but not IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition to peripheral blood parameters ( lymphocyte subpopulations , neopterin , beta 2 microglobulin , TNF , IL 1 , IFN gamma ) we recorded in vitro cellular activity by incubating the patients ' peripheral blood mononuclear cells ( PBMC ) in media containing fetal calf serum ( FCS ) or autologous serum , and either IL 2 or buffer . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Less than one in a thousand mononuclear cells obtained directly from IM blood and stained within 2 h of sampling produced IL 2 , interferon gamma ( IFN gamma ) , IL 4 , IL 5 , IL 6 , IL 10 , GM CSF , tumour necrosis factor alpha ( TNF alpha ) or TNF beta , spontaneously . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Analysis of the lung cell cytokine profile seen after ConA mitogenesis showed that after 6 weeks of F . rectivirgula treatments , nonparenchymal cells secreted high levels of tumor necrosis factor alpha ( TNF alpha ) and granulocyte macrophage colony stimulating factor ( GM CSF ) , whereas similar cells from the lungs of mice given 9 12 weeks of treatment secreted larger amounts of interferon gamma ( IFN gamma ) and interleukin 2 ( IL 2 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although the combination of chronic hyperthermia ( 42 degrees C ) with TNF alpha , IFN gamma , or TNF alpha + IFN gamma increased cytotoxicity , alteration / induction of polypeptides was not correlated with the synergistical cytotoxic effects of cytokine and heat . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cells derived from human neuroblastomas were used as a model for investigating the molecular basis for the paucity of MHC class 1 and beta 2 M gene expression in neural cells and for the induction of these genes by two cytokines , IFN gamma , and TNF alpha . ^^^ IFN gamma or TNF alpha also increased MHC class 1 and beta 2 M steady state RNA levels and the expression of MHC class 1 and beta 2 M CAT reporter constructs transiently transfected into the neuroblastoma cell lines , indicating that the cytokines acted by increasing the transcription of these genes . ^^^ TNF alpha increased factor binding activity specific for the NF kappa B like elements and IFN gamma increased factor binding activity specific for the IFN consensus sequence elements of the MHC class 1 and beta 2 M genes , but not vice versa . ^^^ Taken together , our results indicated that IFN gamma and TNF alpha increased MHC class 1 and beta 2 M gene expression in the neuroblastoma cell lines by inducing factor binding to the regulatory elements present in both genes . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Consistent with this hypothesis , activation of microglia with IFN gamma plus TNF alpha or transforming growth factor beta inhibited , in a dose dependent manner , T . gondii multiplication . ^^^ These findings suggest that the host defense function of microglia against T . gondii is dependent primarily on the activating properties of IFN gamma and TNF alpha , and that agents that interfere with cytokine mediated activation may promote the pathogenesis of this infection . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Astrocyte cell cultures were established from human fetal brains and exposed to human recombinant tumor necrosis factor alpha ( TNF alpha ) and human recombinant interferon gamma ( IFN gamma ) , and the proliferative response was assessed by bromodeoxyuridine immunostaining technique . ^^^ TNF alpha showed an inhibitory activity , while IFN gamma induced a slight increase in the number of astrocytes undergoing cell division . ^^^ Previous studies have demonstrated that TNF alpha and IFN gamma are potent mitogens for adult astrocytes in both in vivo and in vitro conditions . ^^^ Our results suggest that in developing brain the immunomodulatory activity of cytokines , TNF alpha and IFN gamma , is different from that observed in adult brain . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These effects were independent of catalase and superoxide dismutase and were not abrogated by antibody against IL 1 , IL 8 , TNF alpha , IFN gamma or IFN alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The purpose of this study was to determine whether tumor necrosis factor alpha ( TNF alpha ) and gamma interferon ( IFN gamma ) were capable of modulating epidermal growth factor receptor ( EGFr ) immunorecognition on a human melanoma cell line in vitro . ^^^ A dose and time dependent enhancement in EGFr immunorecognition was measurable in TNF alpha and IFN gamma treated cells . ^^^ Combinations of these cytokines enhanced the recognition of EGFr on DX 3 cells to a level greater than that achieved with either TNF alpha or IFN gamma alone . ^^^ Scatchard analysis of receptor binding curves revealed that there was no significant change in Mab affinity between control and cytokine treated DX 3 melanoma cells , whereas a 1 . 5 to 1 . 8 fold enhancement in the number of Mab binding sites was measurable in TNF alpha and IFN gamma treated cells , respectively , when compared with controls . ^^^ Immune complex kinase assay of EGFr showed threefold higher tyrosine kinase activity in TNF alpha treated cells , but no change in kinase activity was observed following IFN gamma treatment . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Preincubation of PMN with IFN gamma significantly inhibited their ability to release IL 8 upon stimulation with TNF , LPS , FMLP and Y IgG , but enhanced the respiratory burst capability in response to FMLP and TNF . ^^^ TNF , LPS and FMLP induced expression of IL 8 mRNA was also selectively inhibited by IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Malaria antigen , interferon gamma ( IFN gamma ) and tumour necrosis factor ( TNF ) induced RNI production in macrophages in a dose dependent way . ^^^ RNI production steadily increased over a 2 day period and was enhanced when the malaria antigen was co incubated with IFN gamma and / or TNF . ^^^ L N monomethyl arginine reduced malaria antigen , IFN gamma and TNF induced RNI production when these reagents were used in combination or alone . ^^^ An anti TNF monoclonal antibody ( mAb ) reduced IFN gamma induced RNI production , but did not significantly alter the malaria antigen induced RNI synthesis by macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Optimal IFN gamma production was dependent on the presence of TNF alpha , inasmuch as addition of antibody to TNF alpha to spleen cell or NWNA cell cultures significantly reduced IFN gamma production . ^^^ These findings document the existence of a T independent mechanism for early IFN gamma production in response to S . typhimurium , and show that TNF alpha is necessary but not sufficient for the production of IFN gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Addition of neutralizing mAb against TNF alpha abolishes the IFN gamma response of scid spleen cells exposed to the parasite or of NK cells incubated with supernatants of adherent cells stimulated with T . gondii extract . ^^^ Nevertheless , TNF alpha alone is not sufficient to trigger IFN gamma production from purified NK cell populations . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Picolinic acid also augmented the IFN gamma dependent expression of TNF alpha mRNA , but did not appreciably affect the secretion of the TNF alpha protein . ^^^ Although IL 4 inhibited the expression of IFN gamma plus picolinic acid induced TNF alpha mRNA and protein , it only partially suppressed picolinic acid dependent NO 2 production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In an attempt to increase TNF production , TIL were transduced with a mutated form of TNF containing the IFN gamma signal peptide in place of the transmembranous region , to enhance secretion into the endoplasmic reticulum . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Antiviral effect of TNF alpha and IFN gamma secreted from a CD8+ influenza virus specific CTL clone . ^^^ We observed that an influenza specific cytotoxic T lymphocyte ( CTL ) clone ( B7B7 ) stimulated with peptide antigen could produce TNF alpha and IFN gamma simultaneously . ^^^ The culture supernatant containing both TNF alpha and IFN gamma of antigen stimulated CTL clone B7B7 significantly enhanced the lysis of influenza A / PR / 8 virus infected L M2d6 cells or Meth A cells . ^^^ Enhanced lysis of influenza virus infected cells by the supernatants was inhibited by pretreatment of the supernatant with antimurine TNF alpha antibody and antimurine IFN gamma antibody . ^^^ These results suggest that the protective mechanism mediated by TNF alpha and IFN gamma secreted from CTL may be possible in the course of an influenza infection . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha strongly synergized with interferon gamma ( IFN gamma ) and interleukin 4 ( IL 4 ) . ^^^ On human blood monocytes , TNF alpha , like IFN gamma or IL 6 , counteracts the IL 4 induced Fc epsilon RII expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The TMF phenotype changed during cultivation and upon stimulation with inflammatory cytokines ( IL 1 , IFN gamma , TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
All T cell lines from healthy individuals and TT patients responding to M . leprae , M . tuberculosis , or individual Ag , produced high levels of IFN gamma and TNF alpha but little or no IL 4 and IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The production of colony promoting activity was observed when fibroblasts were stimulated by lipopolysaccharide ( LPS ) or individual cytokines [ IL 1 , interferon gamma ( IFN gamma ) , IL 2 , IL 4 or TNF ] in serum free conditions as well as by serum itself . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Other cytokines implicated as signals in neuroimmune interactions are IL 2 , IL 6 , IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 alpha ( IL 1 alpha ) , and interferon gamma ( IFN gamma ) at low doses showing < 50 % inhibition of cell growth by themselves enhanced the susceptibility of the cells to the activity of 5 ' dFUrd . ^^^ TNF alpha , IL 1 alpha , IFN gamma , and a mixture of these factors increased the enzyme activity by up to 3 . 7 fold in colon 26 cells . ^^^ Tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 alpha ( IL 1 alpha ) , and interferon gamma ( IFN gamma ) at low doses showing < 50 % inhibition of cell growth by themselves enhanced the susceptibility of the cells to the activity of 5 ' dFUrd . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
One and two level regulation patterns affecting NF kappa B mRNA and nuclear NF kappa B activity after treatment with TNF alpha , IFN gamma and IL 4 . ^^^ We also used known inducers of differentiation like TNF alpha , IFN gamma and IL 4 that interact via their corresponding surface receptors found on HL 60 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Anti tumor necrosis factor alpha ( anti TNF alpha ) or anti interferon gamma ( anti IFN gamma ) monoclonal antibodies did not inhibit ( IL 2 + GM CSF ) induced LAK generation , indicating that GM CSF augmentation does not require TNF alpha or IFN gamma activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The production of the cytokines interferon gamma ( IFN gamma ) , interleukin 1 alpha ( IL 1 alpha ) and tumor necrosis factor alpha ( TNF alpha ) was investigated in the mitogen stimulated whole blood cell culture media from 51 patients with urinary bladder carcinomas , 52 patients with renal carcinomas , 31 patients with prostatic carcinomas and 360 healthy controls . ^^^ In the blood cell culture supernatants of the patients with urinary bladder carcinomas significant lower levels of IFN gamma ( P < or = 0 . 001 ) , IL 2 ( P < or = 0 . 01 ) and TNF alpha ( P < or = 0 . 05 ) were found as compared to the controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The production of interleukin 1 alpha ( IL 1 alpha ) , IL 2 , IL 3 / granulocyte macrophage colony stimulating factor ( GM CSF ) , IL 4 , IL 5 , IL 6 , tumor necrosis factor alpha ( TNF alpha ) , TNF beta and interferon gamma ( IFN gamma ) by some of the cell lines was detected , and no cell line produced IL 1 beta or IFN alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We show here that a single dose of TNF alpha causes significant small intestinal pathology in normal adult mice , which develops within 15 minutes , persists for up to 48 hours and is enhanced by interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cancer patients undergoing interleukin ( IL ) 2 based immunotherapy frequently experience dose limiting side effects believed to be caused by the actions of such cytokines as IL 1 beta , tumor necrosis factor ( TNF ) alpha and beta , and interferon gamma ( IFN gamma ) . ^^^ The secretions of IL 1 beta , TNF alpha , and beta , and IFN gamma induced by either R38A or F42K were markedly reduced compared with secretions produced in response to recombinant wild type IL 2 . ^^^ In 4 experiments , secretion was reduced an average of 39 % for IL 1 beta , 57 % for TNF alpha , 83 % for TNF beta , and 86 % for IFN gamma . ^^^ These data suggest the interaction of IL 2 and the high affinity IL 2 receptor on human PBMC or peripheral blood lymphocyte is required for maximal secretion of IL 1 beta , TNF alpha , TNF beta , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS Reverse transcriptase and the polymerase chain reaction were used to amplify mRNA for interleukins 1 ( IL 1 ) , 2 ( IL 2 ) , 4 ( IL 4 ) and 6 ( IL 6 ) , IL 2 receptor ( IL 2R ) , tumour necrosis factors alpha ( TNF alpha ) and beta ( TNF beta ) , gamma interferon ( IFN gamma ) and granulocyte macrophage colony stimulating factor ( GM CSF ) in frozen punch biopsy specimens of skin and necropsy samples of skin , lymph node , and spleen . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There was also decreased infiltration by macrophages ( 18 + / 8 vs . 37 + / 8 cells / HPF , P < 0 . 01 ) , T cells ( 5 + / 3 vs . 19 + / 7 , P < 0 . 01 ) , and IL 2R+ T cells ( 5 + / 3 vs . 15 + / 4 , P < 0 . 01 ) , and a significant reduction in the numbers and extent of intragraft mononuclear cells stained with antibodies to IL 1 , IL 2 , IL 6 , IL 8 , IFN gamma , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cell lines 1308 . 1 and 6 . 1 . 1 also produce IL 7 ; 6 . 1 . 1 produces IL 1 beta and tumor necrosis factor ( TNF ) alpha while the 427 . 1 cell line produces IL 5 and IFN gamma mRNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The biochemical characteristics of ELDF and blocking experiments using cytokine specific neutralizing antibodies suggest that ELDF is different from gamma interferon ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 5 ( IL 5 ) and interleukin 2 ( IL 2 ) , which may exist in HIL 3 sup , and that ELDF may be a previously unrecognized leukemia differentiation factor . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Acquisition of the augmented capacity for TNF production was due to L . monocytogenes induced IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vivo , interferon gamma ( IFN gamma ) and TNF alpha serum levels of infected euthymic mice remained below the level of detectability . ^^^ Treatment of infected nude mice , which developed chronic infection , with discontinuous injections of IFN gamma , TNF alpha , or both did not decrease bacterial colony forming units in liver , spleen , or lungs . ^^^ However , treatment of infected euthymic mice , which cured a R . equi inoculum within 3 weeks , with anti IFN gamma or anti TNF alpha antibodies ( or both ) significantly increased tissue colony counts . ^^^ These data argue that , in this murine model , endogenous IFN gamma and TNF alpha are involved in the cell mediated immunologic response against R . equi infection . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
One group consisted of interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and interleukin 10 ( IL 10 ) , for which mRNAs were induced within 1 day after challenge . ^^^ Cells expressing TNF alpha and IL 1 alpha mRNA were present throughout liver granulomas , whereas cells that expressed IFN gamma mRNA were observed mostly along the periphery of granulomas . ^^^ We conclude that cytokine mRNA expression during the early phases of L . monocytogenes infection in mice is temporally regulated and that IFN gamma , TNF alpha , and IL 1 alpha are expressed by cells associated with hepatic granulomas . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1 , TNF alpha and , in addition , interferon gamma ( IFN gamma ) raised the M CSF levels within 6 h ; cycloheximide potentiated the effects of IL 1 and TNF alpha on mRNA levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These supernatants were tested for their interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) , and interleukin 6 ( IL 6 ) concentration . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of GM CSF or M CSF derived macrophages with interferon gamma ( IFN gamma ) ( 50 U / ml ) , tumor necrosis factor ( TNF ) ( 500 U / ml ) , interleukin 1 ( IL 1 ) ( 200 U / ml ) or interleukin 6 ( IL 6 ) ( 100 U / ml ) for 24 h rendered them significantly cytotoxic to different tumor cells . ^^^ Medium derived macrophages showed little cytotoxicity against tumor cells and production of TNF on treatment with TNF , IFN gamma , IL 1 or IL 6 . ^^^ Out of the different combinations tested , only IFN gamma plus TNF treated macrophages showed enhancement in nitrite production as compared to that of IFN gamma alone . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inflammatory mediators such as IL 1 , IL 6 , TNF alpha , and IFN gamma produced within the allograft can increase the local expression of adhesion molecules and thereby promote the entry of specific and nonspecific cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Quantitation of cytokine mRNA in SEA activated mononuclear cells by the reverse transcriptase polymerase chain reaction ( RT PCR ) revealed strong induction of mRNA encoding the cytokines IL 1 alpha , IL 1 beta , IL 2 , IL 6 , TNF alpha , TNF beta and IFN gamma . ^^^ Addition of recombinant cytokines to WiDr colon carcinoma cells showed that TNF alpha was able to act synergistically with IFN gamma to suppress tumor cell growth . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both immunohistochemical analysis and the reverse transcripts polymerase chain reaction ( RT PCR ) were used to analyze the expression of VCAM 1 , ICAM 1 , ELAM 1 , very late antigen 4 ( VLA 4 [ alpha 4 , beta 1 ] ) , lymphocyte function associated antigen 1 ( LFA 1 ) , interferon gamma ( IFN gamma ) , tumor necrosis factor ( TNF ) , and interleukin 1 beta ( IL 1 beta ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1 alpha , IL 4 , IL 6 , IL 10 , and IFN gamma levels were decreased by treatment with low doses of DEX ( 30 mg / kg ) , whereas higher doses were required to inhibit production of IL 2 , IL 3 , and TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum levels of interleukin 1 ( IL 1 beta ) , interleukin 6 ( IL 6 ) , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and C reactive protein ( CRP ) were investigated in patients with chronic liver diseases ( CLD ) and correlated with the type of underlying disease and various clinical and laboratory parameters . ^^^ Serum levels of IL 1 beta , IL 6 , TNF alpha , IFN gamma , and CRP were elevated in patients with CLD . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Injection of anti CD 3 MAb induced rapid production of endogenous tumor necrosis factor ( TNF ) in the spleens and endogenous gamma interferon ( IFN gamma ) in the bloodstreams and spleens of mice . ^^^ Alternatively , in vivo depletion of anti CD 3 MAb induced TNF and IFN gamma by the simultaneous administration of antibodies against TNF and IFN gamma suppressed anti CD 3 MAb induced antilisterial resistance . ^^^ These results suggest that the preventive effect of anti CD 3 MAb might be due to activation of phagocytes by TNF and IFN gamma induced by stimulating CD4+ T cells and CD8+ T cells with the MAb . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation of PBMC in the presence of neutralizing anti IL 10 mAb indicated that endogenous IL 10 production inhibits PBMC proliferation and release of TNF alpha , GM CSF , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Short exposures to tumor necrosis factor ( TNF ) alpha also enhanced the CL responses of PMNL , but the effects IFN gamma and TNF alpha together , at the concentrations used , were less than additive . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha production was significantly reduced during thalidomide treatment while interferon gamma ( IFN gamma ) production was enhanced . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The mRNA expression of interleukin ( IL ) 1 beta , IL 2 , IL 3 , IL 4 , IL 6 , IL 6R , IL 9 , IL 10 , IL 12 , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and transforming growth factor beta ( TGF beta ) was analyzed by a sensitive reverse transcriptase polymerase chain reaction technique in 10 SS tissue samples from 10 consecutive patients [ 7 nonmalignant parotid myoepithelial sialadenitis ( MESA ) lesions with evidence of B cell clonal expansion , and 3 B cell non Hodgkin ' s lymphomas ] as well as a series of 11 SS unrelated B cell non Hodgkin ' s lymphomas . ^^^ IL 1 , IL 2 , IL 3 , IL 6 , IL 6R , IL 10 , IL 12 , IFN gamma , TNF alpha , and TGF beta mRNA was expressed in all or in the vast majority of the samples analyzed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To investigate the effects of human interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) , either alone or in combination , on the viability of human muscle cells in culture . ^^^ Cultures of human muscle cells were treated with various concentrations of recombinant IFN gamma and TNF alpha alone and in combination , and the cytotoxic effects of the cytokines on muscle cells were assessed by measuring lactic dehydrogenase ( LDH ) release in supernatants and by observation of the cells for morphologic changes under phase microscopy . ^^^ Exposure of muscle cells to 100 U / ml of either IFN gamma or TNF alpha for 9 days caused no cytotoxic effects , as assessed by LDH release in supernatants of muscle cell cultures and by microscopic observation of the cell cultures . ^^^ However , when IFN gamma and TNF alpha were added together in the muscle cell cultures , they caused significant cytotoxic effects . ^^^ Thus , in combination , IFN gamma and TNF alpha at 100 U / ml each caused significant release of LDH ( 3rd day 9 % , 4th day 28 . 5 % , 7th day 55 . 5 % , 9th day 74 % ) in the supernatants of treated cultures compared to controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our results demonstrate that the CAEV LTR is activated by treatment of immature monocytes with gamma interferon ( IFN gamma ) or a phorbol ester but not with tumor necrosis factor alpha or lipopolysaccharide . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum levels of TNF alpha , IFN gamma , and IL 6 were also increased during VOD and aGVHD , with peak levels occurring at the same time as peak sIL 2R levels in most patients . ^^^ Inflammatory cytokines like IL 6 , TNF alpha , and IFN gamma also increased during VOD and aGVHD . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
By using ELISAs specific for mouse cytokines , large amounts of IL 1 alpha , TNF alpha , IL 6 , and IFN gamma were detected in the peritoneal washings of huPBL SCID mice 1 day after intraperitoneal injection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In preliminary experiments with fluorescence microscopy we found that neither TNF alpha nor IL 1 beta induce LFA 3 in the same fashion as IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Activation of MME with TNF alpha and IFN gamma resulted in a strong elevation of GAPDH mRNA levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha and IFN gamma mediated signal transduction pathways : effects on glial cell gene expression and function . ^^^ This article focuses on the biological role of two cytokines , namely : tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) , in the progression of neurologic disorders such as multiple sclerosis ( MS ) and AIDS dementia complex ( ADC ) , with an emphasis on cytokine effects on glial cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
ICAM 1 is constitutively expressed by neonatal rat astrocytes , and expression is enhanced by the proinflammatory cytokines interleukin 1 beta ( IL 1 beta ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) , with IL 1 beta and TNF alpha being the strongest inducers . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These data suggest that some forms of androgen independent prostate cancer may benefit from a combination therapy of IFN gamma and TNF alpha , while the use of IFN gamma alone may be more efficacious in others . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokines ( IFN alpha , IFN gamma , and TNF alpha ) from stimulated peripheral blood mononuclear cells ( PBMC ) were measured using monoclonal IFN antibodies and ELISA test . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Thus , we further determined the presence in the PBMNC conditioned medium ( CM ) of both inhibitory cytokines : macrophage inflammatory protein 1 alpha ( MIP 1 alpha ) , tumor necrosis factor alpha ( TNF alpha ) , transforming growth factor beta 2 ( TGF beta 2 ) , and interferon gamma ( IFN gamma ) , and stimulatory cytokines : interleukin 3 ( IL 3 ) and stem cell factor ( SCF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In cultures from FIS 2 infected mice , the antibody response was reduced compared with cultures from uninfected mice and the production of the Th 2 cytokines IL 4 and IL 6 was elevated , whereas the Th 1 cytokines IL 2 , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) were reduced . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The T cells , when activated via the T cell receptor ( TCR ) / CD3 complex , were suppressed functionally by minocycline , resulting in a dose dependent inhibition of T cell proliferation and reduction in production of IL 2 , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Thus , we further determined the presence in the PBMNC conditioned medium ( CM ) of inhibitory cytokines ( macrophage inflammatory protein 1 alpha [ MIP 1 alpha ] , transforming growth factor beta 2 [ TGF beta 2 ] , interferon gamma [ IFN gamma ] , and tumor necrosis factor alpha [ TNF alpha ] ) and stimulatory cytokines ( granulocyte macrophage colony stimulatory factor [ GM CSF ] , interleukin 3 [ IL 3 ] , and stem cell factor [ SCF ] ) . ^^^ After PHA stimulation , the production of MIP 1 alpha , IFN gamma , TNF alpha , and GM CSF significantly increased in the cultures of AA patients ( p = 0 . 0009 , 0 . 0002 , 0 . 0022 , and 0 . 0156 , respectively ) . ^^^ Furthermore , the myelopoietic suppressing effect of AA PBMNC CM could be significantly blocked by pretreatment with specific antibodies to the corresponding inhibitory cytokines ( MIP 1 alpha , IFN gamma , and TNF alpha ) . ^^^ After antibody neutralization , an apparent change occurred in the clonal growth of normal BM cells incubated with AA PBMNC CM , resulting in colony enhancement of 205 , 131 , and 237 % by anti MIP 1 alpha , anti IFN gamma , and anti TNF alpha , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , other TCC produced IFN gamma and TNF but no IL 4 , or IL 5 , compatible with a Th 1 like pattern . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Incubation of isolated purified cardiac myocytes from normal rats for 24 h with cytokines known to be present during allograft rejection ( IL 1 beta , TNF alpha and IFN gamma ) was also associated with increased iNOS mRNA and enzyme activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Culture of B . burgdorferi in cytokine conditioned media indicated that TNF alpha , IFN gamma , and IL 2 were not cytotoxic for B . burgdorferi . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mouse luteal cells were cultured in serum free medium with interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) or interleukin 1 beta ( IL 1 beta ) alone , or with various combinations of these cytokines for 6 days . ^^^ IFN gamma ( 1000 U / ml ) , TNF alpha ( 3000 U / ml ) , or IL 1 beta ( 30 U / ml ) alone , and the combination of TFN alpha and IL 1 beta ( 10 U / ml ) did not decrease number of viable cells and was without effects on NO production . ^^^ Combinations of INF gamma and TNF alpha , and IFN gamma , TNF alpha and IL 1 beta ( 10 U / ml ) markedly decreased number of viable cells . ^^^ The combination of IFN gamma and TNF alpha increased NO production a little but significantly , and the combination of three cytokines ( IFN gamma , TNF alpha , and IL 1 beta ) caused a greater increase in NO production . ^^^ An NO synthase inhibitor , L NG monomethy L arginine ( 0 . 5 mM ) or aminoguanidine ( 0 . 5 mM ) abolished increases in NO production induced by combinations of IFN gamma and TNF alpha , and IFN gamma , TNF alpha and IL 1 beta completely without effects on number of viable cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro sensitivity of post bone marrow transplantation CFU GM and BFU E to TNF alpha and IFN gamma . ^^^ In an effort to delineate a cytokine mediated mechanism of secondary graft failure , we investigated colony forming unit granulocyte / macrophage ( CFU GM ) and burst forming unit erythroid ( BFU E ) growth and pattern of inhibition by tumor necrosis factor alpha ( TNF alpha ) and gamma interferon ( IFN gamma ) in the early posttransplant period ( day 28 ) . ^^^ Although early posttransplant BMMNC was more sensitive to inhibition than NLD derived BMMNC , overall , no difference in colony growth or percent of inhibition induced by TNF alpha or IFN gamma was observed between recipients of unmodified and T . cell depleted transplants . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We therefore investigated in vivo and in vitro production of interferon gamma ( IFN gamma ) , soluble interleukin 2 ( IL 2 ) receptor alpha chain ( sCD 25 ) , tumor necrosis factor alpha ( TNF alpha ) , and granulocyte macrophage colony stimulating factor ( GM CSF ) in patients before and during various time periods up to 2 years after autologous and allogeneic BMT . ^^^ The IFN gamma production defects in both patient groups seem to be specific , as sCD 25 , TNF alpha , and GM CSF production in stimulated PBMNC is normal or even enhanced at any time after autologous or allogeneic BMT . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , ouabain provided a complementary signal for either TNF or IFN gamma in inducing iNOS expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Roles of Mac 1 , endogenous TNF alpha , and IFN gamma in pathogenesis of hepatic warm ischemia reperfusion injury ] . ^^^ The roles of neutrophil Mac 1 ( CD11b / 18 ) adhesion molecule , TNF alpha and IFN gamma in hepatic warm ischemia reperfusion injury ( IRI ) were investigated with a newly established mouse model . ^^^ Pretreatment with anti mouse TNF alpha or anti mouse IFN gamma mAb did not affected any parameters . ^^^ However , TNF alpha and IFN gamma were not considered to play a pivotal role in the pathogenesis of the injury and in the regulation of the neutrophils adhesion via Mac 1 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using 10 or 100 ng LPS / ml and 100 U IFN gamma / ml in RPMI 1640 medium plus 0 . 5 % BSA , both cytoplasmic TNF alpha mRNA and TNF alpha precursor and extracellular TNF alpha production by mouse macrophages were increased when compared to stimulation by LPS plus IFN gamma in medium without BSA and FBS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Correlation with increased IFN gamma and TNF and reduced IL 10 production by placental cells . ^^^ The number of resorptions as assessed by uterine scars was significantly increased in infected C57BL / 6 mice and this was associated with a decreased production by placental cells of the Th 2 cytokines IL 4 and IL 10 and increased production of IFN gamma and TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Infection of non Ag expressing HLA A2+ cell lines A 375 and MDA 231 with the vectors resulted in recognition by Ag specific CTLs as demonstrated by specific target cell lysis and release of cytokines , including IFN gamma , TNF alpha , and granulocyte macrophage CSF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We recently showed that the physiologic anticoagulant molecule , activated protein C ( APC ) , blocks responses of human blood Mphi , alveolar Mphi , or THP 1 cells induced by LPS , IFN gamma , or PMA , including TNF alpha production and down regulation of several LPS binding related proteins . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Monoclonal antibodies against IFN gamma , interleukin 1 beta ( IL 1 beta ) , IL 6 , IL 8 , and tumor necrosis factor alpha failed to block the T cell proliferative responses , but anti IL 2 was strongly inhibitory . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study nitric oxide release from cultured rat microglia was examined by exposing the cells to synthetic beta amyloid peptides ( beta 25 35 and beta 1 40 ) alone and in combination with the cytokines IFN alpha / beta ( 100 U / ml ) , IL 1 beta ( 100 U / ml ) , TNF alpha ( 100 U / ml ) , TNF beta ( 100 U / ml ) , or IFN gamma ( 10 , 100 , 500 , or 1000 U / ml ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We therefore investigated whether interferon gamma ( IFN gamma ) , a cytokine secreted by T lymphocytes , or interleukin 1 beta ( IL 1 beta ) and tumor necrosis factor alpha ( TNF alpha ) , two cytokines characteristically produced by activated macrophages , can trigger apoptosis of vascular SMCs . ^^^ Simultaneous treatment with IFN gamma and TNF alpha and / or IL 1 beta but not with each cytokine alone promoted death of human and rat SMCs . ^^^ Exposure for 48 hours to a combination of IFN gamma ( 400 U / mL ) , TNF alpha ( 400 U / mL ) , and IL 1 beta ( 100 U / mL ) significantly ( P < . 001 ) increased the accumulation of oligonucleosomes comprising DNA fragments and histones in human SMCs . ^^^ Simultaneous treatment with IFN gamma and TNF alpha or IL 1 beta induced elaboration of nitrite , an end product of nitric oxide , in rat but not human SMCs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lisofylline [ LSF ; ( R ) 1 ( 5 hydroxyhexyl ) 3 , 7 , dimethyl xanthine ] , inhibits the signaling and / or release of certain hematopoietic inhibitory molecules such as tumor necrosis factor alpha , macrophage inflammatory protein 1 alpha , transforming growth factor beta , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Anti tumor necrosis factor alpha ( TNF alpha ) antibodies also inhibited IFN gamma production , and the combination of both anti IL 12 and anti TNF alpha could totally inhibit production of IFN gamma . ^^^ Taken together , these results indicate that the stimulation of IFN gamma production by bacterial DNA is mediated by IL 12 and TNF alpha and point to macrophages / monocytes as targets of action of this macromolecule . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and interferon alfa ( IFN alpha ) each reduced luciferase activity by 40 % . ^^^ Tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and interferon alfa ( IFN alpha ) each reduced luciferase activity by 40 % . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After 48 hr of exposure to HLA class 1 modulating cytokines , Lmp 7 mRNA levels in JEG 3 cells were markedly increased by two interferons ( IFN beta , IFN gamma ) and tumour necrosis factor ( TNF ) whereas at the same time point , Jar cell Lmp 7 mRNA was modestly enhanced by IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , resistant C57BL / 6 mice also expressed high levels of mRNA of IFN gamma ( a Th 1 marker ) and low levels of mRNA of IL 4 ( a Th 2 marker ) in the spleen , whereas susceptible A / J mice had low levels of IFN gamma mRNA but high levels of TNF alpha mRNA in the liver and had high levels of TNF alpha protein in serum , as measured by enzyme linked immunosorbent assay , later during infection just before death occurred . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
DC induced antitumor effects were completely blocked by coadministration of neutralizing monoclonal antibody directed against T helper cell 1 associated cytokines ( such as IL 12 , tumor necrosis factor alpha , IFN gamma ) , and eventually , but not initially , blocked by anti mIL 4 mAb . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
First , the changes in the level of TNF alpha , IFN gamma , IL 6 and IL 10 in the lungs and spleen during the course of MAC infections was examined . ^^^ Tissue levels of TNF alpha and IL 10 increased around weeks 2 to 4 , then rapidly decreased thereafter , and returned to the normal levels by week 8 , while levels of IFN gamma and IL 6 remained very low throughout the observation period . ^^^ Second , effect of either anti TGF beta or anti IL 10 antibody on intracellular growth of MAC in human monocytes cultured in vitro in the medium with or without addition of TNF alpha or IFN gamma were examined . ^^^ Effects of TNF alpha and IFN gamma in reducing the bacterial growth was potentiated by the addition of either anti TGF beta or anti IL 10 antibody . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Like DMSO , all interferons potentiated TNF induced cytotoxicity , IFN beta 1 and IFN gamma being the most potent in this respect . ^^^ When applied together , DMSO and IFN gamma enhanced TNF mediated cell lysis in either an additive ( in the case of U 937 , THP 1 , HL 60 cells ) or a synergistic ( in the case of KG 1 ) manner , suggesting that the mechanisms of the potentiating activity of DMSO and IFN gamma are different . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The proliferative response of spleen cells to parasite antigen was measured by uptake of 3H thymidine and , in parallel , supernatants were removed from cells to measure levels of IFN gamma , TNF , IL 2 and IL 4 by ELISA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , tenidap was found to inhibit IFN gamma production as well as the induction of mRNA encoding IFN gamma or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of RPE cells with lipopolysaccharide ( LPS ) or recombinant tumor necrosis factor alpha , IL 1 , or IFN gamma resulted in a significant level of secretion of IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated whether gamma interferon ( IFN gamma ; a Th 1 cytokine ) , tumor necrosis factor alpha ( TNF alpha ) , and interleukin 4 ( IL 4 ; a Th 2 cytokine ) modulate nitric oxide ( NO ) production in vivo during blood stage infection with Plasmodium chabaudi AS . ^^^ Treatment of resistant C57BL / 6 mice , which resolve infection with P . chabaudi AS and produce increased levels of IFN gamma , TNF alpha , and NO early during infection , with anti IFN gamma plus anti TNF alpha monoclonal antibodies ( MAbs ) resulted in a reduction of both splenic inducible NO synthase mRNA and serum NO 3 levels by 50 and 100 % , respectively . ^^^ Treatment with the anti TNF alpha MAb alone reduced only serum NO 3 levels by 35 % , and treatment with the anti IFN gamma MAb alone had no effect on NO production by these mice during infection . ^^^ Susceptible A / J mice , which succumb to infection with P . chabaudi AS and produce increased levels of IL 4 but low levels of IFN gamma , TNF alpha , and NO early during infection , were treated with an anti IL 4 MAb . ^^^ In addition , our results also demonstrate that treatment of resistant C57BL / 6 mice with anti IFN gamma plus anti TNF alpha MAbs affects , in addition to NO production , other traits of resistance to P . chabaudi AS malaria such as the peak level of parasitemia and the development of splenomegaly . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor ( TNF ) , interleukin 1 alpha ( IL 1 alpha ) , interferon gamma ( IFN gamma ) levels in serum , PLF , bronchoalveolar lavage fluid ( BALF ) , and cell culture supernatants were measured . ^^^ Production of systemic TNF was greater , but the IFN gamma response was diminished in the TPN group compared with the TEN group after intraperitoneal bacterial challenge . ^^^ Tumor necrosis factor ( TNF ) , interleukin 1 alpha ( IL 1 alpha ) , interferon gamma ( IFN gamma ) levels in serum , PLF , bronchoalveolar lavage fluid ( BALF ) , and cell culture supernatants were measured . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation of IFN gamma treated monocytes 6 days postinfection with tumor necrosis factor alpha ( TNF alpha ) , which is known to augment HIV replication in the infected cells , did not result in the induction of the HIV indicating the absence of latent HIV infection in IFN gamma treated monocytes . ^^^ Treatment of monocytes with IFN gamma , TNF alpha , or with a combination of the two agents which is known to induce antimicrobial free radical nitric oxide ( NO 2 in the murine system did not induce NO 2 production human monocytes suggesting the antiviral activity of IFN gamma to be independent of NO 2 ( ) mediated killing of HIV or HIV infected monocytes . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines , such as interleukin 1 ( IL 1 ) alpha and beta , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and interleukin 6 ( IL 6 ) were assayed in a culture supernatant of pericardial fat tissue . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the current study we examined the effect of several other inflammatory cytokines interleukin 1 ( IL 1 beta ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) on synthesis of SI in Caco 2 cells , examined the possibility that inflammatory cytokines affect the synthesis of other enterocyte integral membrane proteins using lactase as a prototype , and examined the possibility that SI gene expression was down regulated in villous enterocytes in vivo during the local inflammatory response of Crohn ' s disease . ^^^ The results show that IL 6 and IFN gamma each mediate a decrease and TNF alpha mediates an increase in synthesis of SI in Caco 2 cells . ^^^ The magnitude of down regulation by IL 6 and IFN gamma is significantly greater than the up regulation by TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expressions of the predominantly T cell derived cytokines ( interleukin ( IL ) 2 , IL 4 , IL 5 , and interferon gamma ( IFN gamma ) , of the predominantly macrophage derived cytokines ( IL 1 , IL 6 , and tumor necrosis factor alpha ( TNF alpha ) ) , as well as cytokines that can be of either T cell or macrophage origin ( granulocyte macrophage colony stimulating factor ( GM CSF ) and transforming growth factor beta 1 ( TGF beta 1 ) and TGF beta 2 ) , were monitored by the reverse transcriptase PCR method . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Vascular endothelial platelet endothelial adhesion molecule 1 ( PECAM 1 ) expression is decreased by TNF alpha and IFN gamma . ^^^ Treatment of endothelial cells with TNF alpha and / or IFN gamma led to dramatic decreases in steady state levels of PECAM 1 mRNA transcripts . ^^^ Actinomycin D chase experiments demonstrated that cytokines selectively destabilize PECAM 1 mRNA transcripts in bovine endothelial cells , decreasing the PECAM 1 mRNA transcript t1 / 2 from basal values of 15 + / 2 h to 4 + / 1 h in TNF alpha and IFN gamma treated cells ( p < 0 . 005 ) , an effect that appeared to be independent of new protein synthesis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After coculture overnight with IL 2 , the cytotoxic function of NKC was not augmented although a subpopulation proliferated and secreted TNF alpha and IFN gamma into the supernatant . ^^^ In contrast , NKDC cytotoxic activity was enhanced by IL 2 , but proliferated poorly and did not secrete TNF alpha or IFN gamma following IL 2 activation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Antibodies against IL 2 , IL 5 and IL 10 had little or no effect on plaque forming cell ( PFC ) induction ; anti IL 6 , TNF alpha and TGF beta enhanced PFC induction ; anti IL 1 alpha , IL 1 beta , IL 4 , IFN gamma and IL 13 suppressed PFC induction . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RPASM cells were incubated with a mixture of TNF alpha ( 500 units / ml ) and IFN gamma ( 100 units / ml ) for 24 hours . ^^^ RPASM continued to produce large amounts of NO , in the absence of further cytokine stimulation , after a 24 hour exposure to TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , IFN gamma pretreatment prevented the alcohol induced suppression of TNF secretion without affecting the PMNL recruitment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Basal secretion by these cells of IL 6 , together with other cytokines considered critical for pregnancy ( IL 1 beta , TNF alpha and IFN gamma ) , was measured with immunological techniques . ^^^ RESULTS : We found that DSC in culture produce insignificant quantities of IL 1 beta , TNF alpha and IFN gamma , but appreciable amounts of IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our data demonstrate the IFN gamma , TNF alpha and IL 1 alpha can decrease the expression of EGFR on some bladder tumour cell lines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The addition of LPS and IFN gamma increased IL 1 , IL 6 , and TNF alpha levels ( 200 750 % increase ) and reduced GM CSF levels to 45 % of basal levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the production of proinflammatory cytokines ( IL 1 beta , IL 6 , IL 8 , and TNF alpha ) and immunoregulatory cytokines ( IL 2 , IFN gamma , and IL 10 ) in the colonic mucosa of patients with active ulcerative colitis ( UC ) , inactive UC , and non inflammatory bowel disease ( IBD ) colitis by organ culture . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
No increase in expression of any of the pro inflammatory cytokines ( tumor necrosis factor alpha , IL 1 beta , or IL 6 ) was found after trauma , and IFN gamma tended to decrease . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Murine macrophages ( M phi ) are activated either by interferon gamma ( IFN gamma ) or interferon alpha / beta ( IFN alpha / beta ) in combination with bacterial lipopolysaccharide ( LPS ) to induce synthesis of tumor necrosis factor alpha ( TNF alpha ) and nitric oxide synthase ( iNOS ) mRNA synthesis for generation of tumor cytotoxic nitric oxide ( NO ) . ^^^ These data indicate that exogenous IFN gamma induces endogenous synthesis and secretion of IFN alpha / beta by M phi , which appears to act in concert with endogenously synthesized TNF alpha for the autocrine induction of NOS mRNA synthesis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The injection of the 145 2C11 anti CD 3 MoAb in mice induces a polyclonal T cell activation resulting in the release of several cytokines , including interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) . ^^^ As these cytokines are known to be involved in the host defence against Trypanosoma cruzi , we measured serum levels of IFN gamma and TNF alpha after injection of the 145 2C11 MoAb in the course of experimental murine Chagas ' disease . ^^^ Compared with control mice , T . cruzi infected BALB / c mice were found to be primed to secrete very high levels of IFN gamma and TNF alpha from the second and the first week of infection , respectively , up to the chronic phase . ^^^ In vivo cell depletion experiments indicated that CD8+ T cells were responsible for these dramatic hyperproductions of IFN gamma and TNF alpha . ^^^ Finally , we showed that anti CD 3 MoAb injection in T . cruzi infected mice was followed by a massive release of nitric oxide ( NO ) metabolites , which was partially reduced by IFN gamma or TNF alpha neutralization . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
They have indeed revealed a very high prevalence of helminth and several other infections with an extreme immune dysregulation , consisting of : ( 1 ) highly elevated plasma IgE , IgG , placental isoferritin , p 75 soluble TNF receptor ( sTNFR ) levels and very high blood eosinophilia ; ( 2 ) increased secretion from phytohaemagglutinin ( PHA ) simulated peripheral blood mononuclear cells ( PBMC ) of the cytokines IL 2 , IL 4 , IL 10 and p 75 sTNFR , and decreased secretion of interferon gamma ( IFN gamma ) and IL 6 ; ( 3 ) increased and decreased surface expression of p 75 TNFR and IL 6 receptor on lymphocytes , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Northern blot analysis showed that IL 1 alpha and TNF alpha increased the expression of IL 8 mRNA , whereas IFN gamma reduced IL 8 mRNA expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Most of the TNF alpha , IL 2 , IL 12 , and IFN gamma were made by the infiltrating human leukocytes , while the colon carcinoma cells were induced to produce IL 4 , IL 10 , and TNF alpha . ^^^ Up regulation of IFN gamma receptors and TNF R p 60 receptors was found , while the TNF R p 80 receptor was absent . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mice of the susceptible model showed a slight delay in the appearance of cells producing the type 1 cytokines IL 2 and IFN gamma and an earlier appearance of TNF producing cells , in comparison to resistant mice . ^^^ Cells producing IL 2 or IL 10 were transient in their appearance in the spleen while cells producing IL 1 , IL 4 , IL 5 , IL 6 , IFN gamma , TNF , or TGF beta were first detectable in either the acute or post acute stage of the infection and persisted up to 700 days post infection in two different resistant models of the infection . ^^^ Cells producing IFN gamma , TNF alpha and TGF beta were particularly numerous even very late in the infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Fourteen cytokines were assayed ; interleukin 1 alpha ( IL 1 alpha ) , IL 1 beta , IL 1 receptor antagonist ( IL 1ra ) , IL 2 , IL 3 , IL 4 , IL 5 , IL 6 , IL 8 , IL 10 , interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) , TNF beta and granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Addition of lipopolysaccharide ( LPS ) , interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) , or platelet activating factor ( PAF ) at the beginning of culture ' primed ' the monocytes , causing them to maintain a high superoxide response for at least 96 hr . ^^^ The ability of LPS , IFN gamma , TNF alpha or PAF to maintain the high superoxide response was blocked by addition of inhibitors of serine proteases , either 4 ( 2 aminoethyl ) benzenesulphonyl fluoride ( AEBSF ) or 3 , 4 dichloroisocoumarin . ^^^ We conclude that activity of a monocyte serine protease is required to maintain the high superoxide response in monocytes primed with LPS , IFN gamma , TNF alpha , or PAF . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The neutralization of IFN gamma or TNF alpha had no effect on NO production in the cultures of infected mouse spleen cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Other cytokines , such as IL 1 and IFN gamma , appeared secondary to TNF and nitric oxide in the age associated sensitivity to LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Role of neutrophils and lymphocytes in inhibition of a mouse mammary adenocarcinoma engineered to release IL 2 , IL 4 , IL 7 , IL 10 , IFN alpha , IFN gamma , and TNF alpha . ^^^ To compare the reactive mechanisms induced by each cytokine , BALB / c mice were challenged with the parental cells of the syngeneic spontaneous mammary adenocarcinoma TSA , or with TSA cells engineered to release IL 2 , IL 4 , IL 7 , IL 10 , IFN alpha , IFN gamma , and TNF alpha , and the tumor growth area was studied histologically , ultrastructurally , and immunohistochemically . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The efficacy of combination treatment with actinomycin D ( Act D ) , recombinant human tumor necrosis factor alpha ( TNF alpha ) , and recombinant murine interferon gamma ( IFN gamma ) was examined on established MmB 16 melanoma in mice . ^^^ In mice inoculated with 10 ( 6 ) melanoma cells ( into the footpad of the hind limb ) and treated locally with Act D , TNF alpha and IFN gamma , beneficial therapeutic effects were found . ^^^ When initiated 1 week after tumor cell inoculation , the 7 day treatment with all these agents administered together at daily doses : 0 . 2 microgram ( Act D ) , 1 microgram ( TNF alpha ) , and 200 U ( IFN gamma ) resulted in a significant delay of tumor progression in comparison to the therapy that included either Act D alone or TNF alpha in combination with IFN gamma . ^^^ The results of this study demonstrate that combination of regional chemotherapy ( actinomycin D ) and immunotherapy ( TNF alpha / IFN gamma ) may display higher efficacy than either treatment alone and may increase therapeutic index without augmenting toxic effects . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
HIV p 24 ag and cytokine profiles ( IL 1 beta , IL 2 , IL 4 , IFN gamma and TNF alpha ) were determined on supernatants on day 14 . ^^^ PAI was able to induce the production of IFN gamma and TNF alpha while that of IL 4 and IL 1 beta was reduced . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we have investigated cytokine ( IL 1 beta , IL 2 , IL 5 , IL 6 , IFN gamma , TNF alpha ) and T cell surface molecule ( IL 2 receptor , CD 28 , CTLA 4 ) gene expression in two way mixed lymphocyte cultures ( MLC ) enhanced by concanavalin A ( ConA ) to assess whether this is a useful predictive method for severe graft versus host disease ( GVHD ) and graft failure in allogeneic bone marrow transplantation ( allo BMT ) patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This antiviral function is mediated by IFN gamma and TNF alpha secreted by the CTL or by the antigen nonspecific macrophages and T cells that they activate following antigen recognition . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The question was asked whether tumour necrosis factor alpha ( TNF ) is involved in regulation of interferon gamma ( IFN gamma ) production by T cells . ^^^ Preincubation of monocytes with rTNF enhanced their ability to induce IFN gamma production while TNF synthesis inhibitors decreased it . ^^^ Anti TNF and anti TNF R 2 monoclonal antibodies ( mAbs ) inhibited monocyte ability to present PPD for IFN gamma production which suggested that endogenously produced TNF by monocytes had to be released and acted on TNF R 2 on the monocyte surface . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment with monoclonal antibodies to interferon gamma ( IFN gamma ) or TNF alpha blocked tumor regression by inhibiting VCAM 1 and E selectin expression on tumor associated endothelial cells resulting in a reduced number of infiltrating leukocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The levels in two patients with high levels of CSF IFN gamma and TNF alpha were remarkably increased . ^^^ It has been reported that TNF alpha enhances the effect of IFN gamma for neopterin release by macrophages in vitro and that dexamethasone has the same effect on IFN gamma as TNF alpha . ^^^ The present study suggests that elevation of CSF neopterin in bacterial meningitis results from monocytes / macrophages costimulated with IFN gamma , TNF alpha and dexamethasone used in treatment . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This phenomenon was most likely induced by elevated TNF alpha and IFN gamma plasma levels , which were at a maximum shortly after the end of the infusion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Improved T cell activation was noted , as indicated by an increase in IFN gamma , IL 6 , IL 8 , and IL 10 , in addition to high TNF alpha increases . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation of macrophages with IFN gamma or TNF alpha shuts off the suppressive effect played by PGE 2 . ^^^ In this study , carried out on peritoneal macrophages obtained from healthy , tumour bearing and Corynebacterium parvum treated mice , we demonstrated that PGE 2 is involved in the down regulation of macrophage activation , but it can not exert its inhibiting effect when macrophages are further stimulated with activating cytokines , such as IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neither supernatants from lectin stimulated mononuclear WBC , IFN gamma , TNF alpha , IL 8 , nor ROS showed any significant effect on the ionophore challenged AR . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A transcription factor designated differentiation induced factor ( DIF ) , activated by treatment of myeloid cells with the differentiating agents interferon gamma ( IFN gamma ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , colony stimulating factor 1 ( CSF 1 ) or during phorbol ester induced differentiation , was characterized as a 112kDa protein related to , but not identical with known isoforms of STAT 5 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the unprotected BALB / c mice the levels of PGE 2 that were responsible for the depression of interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) Th 1 associated cytokines and for the relative increase in the interleukin 4 ( IL 4 ) became higher and higher as the lesion progressed . ^^^ On the contrary , the cured mice produced levels of PGE 2 similar to normal uninfected controls , high levels of TNF alpha and IFN gamma and low levels of IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pretreatment of endothelial cells with tumour necrosis factor alpha ( TNF alpha ) , but not with interferon gamma ( IFN gamma ) , increased the IgG binding from sera of some patients with active SLE . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine gene expression was investigated by intronspanning RT PCR for IL 1 alpha , IL 1 beta , IL 2 , IL 4 , IL 5 , IL 6 , IL 10 , IFN alpha , IFN beta , IFN gamma , and TNF alpha . ^^^ Germinal centre T cells showed production of IL 2 , IL 4 , IL 10 , IFN gamma , and TNF alpha mRNA . ^^^ The mRNAs of these cytokines could also be detected at the single cell level ; as they were produced in a high percentage of germinal centre T cells , whereas immunohistological staining was negative , we conclude that a high percentage of germinal centre T lymphocytes produce IL 2 , IL 4 , IL 10 , IFN gamma , and TNF alpha in low concentrations in contrast to T cells outside the germinal centre , in which strong cytokine production in few cells was shown earlier . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These cells were examined by the reverse transcriptase polymerase chain reaction ( RT PCR ) using nested internal primer pairs for the following cytokines : CSF 1 , GM CSF , TNF alpha , TGF beta 1 , IFN gamma , IL 2 , LIF and also for LIF receptor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Nonsteroidal anti inflammatory drugs differentially regulate cytokine production in human lymphocytes : up regulation of TNF , IFN gamma and IL 2 , in contrast to down regulation of IL 6 production . ^^^ NSAIDs up regulated TNF , IFN gamma and IL 2 production at both the mRNA and protein levels , and IL 12 expression at the mRNA level . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
For this purpose , these patients ' PBL were co cultured for 28 days either with IL 2 or a mixture of IL 2 , IFN gamma and TNF alpha in the presence of autologous tumour cells ( ATC ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The combined effects of 1 alpha , 25 dihydroxyvitamin D 3 [ 1 , 25 ( OH ) 2D3 ] and interferon gamma ( IFN gamma ) or tumor necrosis factor alpha ( TNF alpha ) on cell differentiation in HL 60 human promyelocytic leukemia cells were examined by a nitroblue tetrazolium ( NBT ) reduction test . 1 , 25 ( OH ) 2D3 at the concentrations of 7 70 nM induced NBT positive cells , which was used as a criterion of cell differentiation . ^^^ Treatment with a combination of 1 , 25 ( OH ) 2D3 and TNF alpha or IFN gamma and TNF alpha showed an additive effect on cell differentiation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The antibacterial competence of human macrophages is lessened by IL 4 and IL 10 and augmented by IFN gamma , TNF alpha , and GM CSF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Specific alpha / beta T cells produce several cytokines that attract and activate macrophages and additional lymphocytes , such as : interferon gamma ( IFN gamma ) which has the capacity to activate several antimicrobial properties of macrophages ; tumour necrosis factor alpha ( TNF alpha ) a key cytokine involved in granuloma formation ; interleukins 2 , 6 and 8 ( IL 2 ; IL 6 and IL 8 ) ; and interleukin 12 ( IL 12 ) , a candidate cytokine for the induction of Th 1 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma treatment enhanced TNF alpha production , but decreased IL 6 production . ^^^ These results suggest that IFN gamma differentiates U 937 cells to monocyte like cells and it regulates the production systems of IL 6 and TNF alpha separately in U 937 cells . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The present study showed these cells to also be in the spleen , lymph node , and tumor , and that treatment of tumor bearing mice with low doses of IFN gamma plus TNF alpha reduced the frequency of E MP12+ cells . ^^^ Intratumoral ER MP12+ cells could be maintained as suppressive blastlike cells for at least 4 days in cultures containing CSFs , but adding IFN gamma plus TNF alpha to these cultures caused their differentiation mainly into nonsuppressive TNF alpha secreting monocytic cells . ^^^ IFN gamma / TNF alpha treatment stimulates their differentiation and shift from production of TGF beta and NO to production of TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In mice , the temporal responses of IL 1alpha , tumor necrosis factor ( TNF ) alpha , IL 2 , and IFN gamma evoked by SEB were synergistically potentiated by lipopolysaccharide ( LPS ) . ^^^ Niacinamide primarily reduced IL 2 and IFN gamma , while IL 10 predominantly reduced IL 1alpha and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma significantly inhibited the production of GRO alpha by PMN stimulated for 2 hr with LPS , TNF alpha , or Y IgG , but potentiated the production of GRO alpha in cells stimulated for 18 hr with LPS and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor cells from 7 freshly isolated human ovarian tumors and 2 continuous human ovarian cancer cell lines were analyzed for their surface expression of MHC class 1 , class 11 and ICAM 1 surface antigens before and after exposure to gamma irradiation and / or the cytokines TNF alpha plus IFN gamma . ^^^ All 7 fresh tumors expressed high levels of MHC class 1 and 1CAM 1 antigens , and levels were markedly up regulated after exposure to TNF alpha plus IFN gamma Similarly , class 11 antigens were either induced ( 3 out of 7 tumors ) or significantly up regulated by TNF alpha plus IFN gamma . ^^^ Exposure of these cells to TNF alpha plus IFN gamma markedly up regulated antigen expression to levels comparable to those expressed on the freshly isolated tumors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
By use of a T cell specific inducible expression system , we show that upon T cell activation , induced nef expression down regulated both IL 2 and IFN gamma production in a dose dependent manner , whereas IL 4 , IL 9 , IL 13 , IL 8 , and TNF alpha production remained unaffected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Schwann cells treated with IFN gamma and TNF alpha upregulated iNOS specific mRNA within 12 hr and released nitrite in a time and dose dependent manner , reaching a plateau of secretion after 3 days . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , IL 1 , IL 4 , IL 6 , IL 7 , IL 12 , tumor necrosis factor alpha ( TNF alpha ) , interferon alpha ( IFN alpha ) and IFN gamma do not support the growth of NKL cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Following intravenous administration of LEH , 1 ) a serum spike of interleukin 6 ( IL 6 ) occurred in mice at 4 8 hours , with no elevation of IL 1 , tumor necrosis factor ( TNF ) , or interferon gamma ( IFN gamma ) ; 2 ) the serum liver function enzymes SGOT ( AST , aspartate aminotransferase ) and SGPT ( ALT , alanine aminotransferase ) were elevated at 48 hours ; 3 ) only a slight increase in serum antibody to bovine hemoglobin was observed ; and 4 ) increased hematopoietic activity was observed in the spleen and bone marrow . ^^^ The finding that only IL 6 but not the associated TNF , IL 1 , or IFN gamma is secreted in vivo following LEH administration is novel and may have significance in defining the mechanisms underlying specific adverse responses observed with LEH administration in animals . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This technique was applied to quantify tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) and interleukin 2 ( IL 2 ) mRNA levels in human T cells transfected with the corresponding genes . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Semiquantitative polymerase chain reaction ( PCR ) comparing CD28+ and CD 28 CD8+ T cells with respect to cytokine gene expression ( tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , IL 1beta ) in five renal transplant patients with expansion of the CD57+ subset detected no cytokine gene expression deficit in CD 28 T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study it is shown that the effects of interferon gamma ( IFN gamma ) , but not of tumour necrosis factor alpha ( TNF alpha ) , are inhibited by treatment with soluble heparin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of HLA DR antigen on Ca and on normal epithelia , both on AN and those 5 10 cm removed from the carcinoma ( RN ) , were examined immunohistochemically . mRNAs of cytokines , IFN gamma and TNF alpha , were detected by reverse transcription polymerase chain reaction ( RT PCR ) in both carcinoma and remote normal tissues . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study , we investigated the ability of OK 432 induced inflammatory cytokines , such as interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and interleukin 1 beta ( IL 1 beta ) , to enhance ICAM 1 expression . ^^^ Treatment of the two cultured tumor cells with recombinant IFN gamma , but not recombinant TNF alpha or IL 1 beta , significantly increased their ICAM 1 expression to a clinically detectable level . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Up to 50 % of the soluble factor mediated suppression is attributable to a gamma interferon ( IFN gamma ) dependent pathway , and the mediator of the remaining inhibition is neither interleukin 10 , transforming growth factor beta , prostaglandin E 2 , lipoxygenase products , nitric oxide , nor tumor necrosis factor alpha induced mitochondrial cell derived reactive oxygen intermediates . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study the roles of the integrins Mac 1 and VLA 4 , as well as those of the cytokines tumor necrosis factor alpha ( TNF alpha ) and gamma interferon ( IFN gamma ) , in defense mechanisms against Yersinia enterocolitica in Peyer ' s patches ( PP ) and mesenteric lymph nodes ( MLN ) were investigated by blocking these molecules with antibodies in vivo prior to orogastric Yersinia infection . ^^^ Neutralization of TNF alpha or IFN gamma had comparable effects , suggesting that cell mediated host responses including activated macrophages are required for control of yersiniae in intestinal tissues . ^^^ While anti VLA 4 , TNF alpha , or IFN gamma antibody treatment caused an increased dissemination of yersiniae from PP to the spleen systemic dissemination was reduced by anti Mac 1 antibodies . ^^^ The results of this study suggest that the cytokines IFN gamma and TNF alpha as well as the integrins Mac 1 and VLA 4 are involved in protective cellular host defense mechanisms in PP and MLN against Y . enterocolitica , the latter probably being involved in both cell cell and cell pathogen interactions . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Circulating levels of proinflammatory cytokines ( tumor necrosis factor alpha ( TNF alpha ) interleukin 1 beta [ IL 1 beta ] , IL 2 , IL 6 , and interferon gamma [ IFN gamma ] ) , anti inflammatory cytokines ( transforming growth factor beta 1 [ TGF beta 1 ] , IL 4 , IL 10 , and IL 13 ) , the cytokine carrier protein alpha 2 macroglobulin , IL 1 receptor antagonist ( IL 1ra ) , soluble TNF receptors ( sTNFr ) p 55 and p 75 , and soluble IL 6 receptor ( sIL 6r ) were determined in 15 patients with POEMS syndrome and 15 with multiple myeloma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have investigated the production of osteoclast activating cytokines ( IL 6 , IL 1alpha and tumour necrosis factor alpha ( TNF alpha ) ) , interferon gamma ( IFN gamma ) and IL 8 by T cells in the affected joint . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Isolated limb perfusion ( ILP ) with TNF alpha , IFN gamma and melphalan causes impressive tumour reduction in patients with irresectable soft tissue sarcomas with a high limb salvage rate . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma , TNF alpha , IL 1 , and granulocyte macrophage CSF ( GM CSF ) play an important role in host resistance to infection with nontyphoid Salmonella . ^^^ In vitro studies suggested that macrophages from Salmonella susceptible mice ( Itys phenotype ) are impaired functionally in their ability to produce , or stimulate the production of , cytokines such as TNF alpha and IFN gamma . ^^^ As reported in this work , the in vivo response to Salmonella dublin infection in both Itys and Ityr mice was characterized by increased expression of IFN gamma , TNF alpha , GM CSF , IL 1 alpha , IL 2 , IL 6 , IL 10 , and IL 12 p 40 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Complement receptor type 3 mediates phagocytosis and killing of Listeria monocytogenes by a TNF alpha and IFN gamma stimulated macrophage precursor hybrid . ^^^ The experiments described here tested the role of CR 3 in phagocytosis and killing of Listeria by a clonal population of TNF alpha / IFN gamma stimulated macrophage precursor hybrids . ^^^ Stimulation with TNF alpha and IFN gamma increased CR 3 expression 20 fold and induced a big increase in phagocytic activity . ^^^ These data suggest that upregulation of CR 3 and CR 3 mediated phagocytosis are mechanisms by which TNF alpha and IFN gamma stimulate nonphagocytic , nonbactericidal macrophage precursors to kill intracellular bacterial pathogens . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increased levels of several cytokines , including interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) , have been demonstrated in the salivary gland microenvironment of patients with Sjgren ' s syndrome ( SS ) . ^^^ This study examined the role of IFN gamma + / TNF alpha on cellular death in a cultured human salivary gland cell line ( HSG ) . ^^^ Cells treated long term with IFN gamma + / TNF alpha demonstrate a profound antiproliferative effect with a decrease in cell number to below that initially plated . ^^^ Treatment of HSG cells with TNF alpha alone did not have any significant effects on growth but did increase the expression of the IFN gamma receptor . ^^^ The maximum percentage of cells exhibiting DNA degradation at 12 days was 58 % for cells treated with IFN gamma + TNF alpha , 31 % for IFN gamma treated cells , and < 5 % for TNF alpha treated and untreated cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The aim of the present work is to elucidate the role of IL 10 during mixed lymphocyte reaction and to investigate the influence of HLA DR antigens on its production and on the regulatory loop involving TNF alpha and IFN gamma . ^^^ This contrasted with TNF alpha and IFN gamma productions , which significantly increased in 2 DR MM pairs . ^^^ We conclude that the 1 DR MM setting is associated with optimal IL 10 secretion and more efficient inhibition of IFN gamma and TNF alpha compared with the 2 DR MM configuration . ^^^ Although promoting enhanced IFN gamma and TNF alpha release , introduction of an additional DR MM does not result in increased IL 10 production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Plasma levels of TNF alpha , IFN gamma , IL 6 , and IL 8 increased as predicted following OKT 3 administration , without significant differences between PTF and placebo groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PBMC and graft infiltrating cells of patients with late aRx showed an upregulation , especially of IFN gamma , IL 4 , IL 10 , and TNFalpha transcripts . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We are interested in the mechanisms that regulate the survival and differentiation of placental trophoblasts and have recently demonstrated that the inflammatory cytokines tumor necrosis factor alpha ( TNF alpha ) and gamma interferon ( IFN gamma ) act in concert to induce apoptotic cell death in normal cytotrophoblasts in culture . ^^^ These observations suggest a novel role for EGF in normal placental development and indicate that the interplay between EGF , TNF alpha , and IFN gamma may determine the rate of trophoblast growth and renewal during gestation . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Budesonide ( 10 ( 7 ) M ) dramatically reduced A PBM proliferation ( measured by 3H thymidine incorporation ) and cytokine ( IL 1beta , IL 2 , IL 6 , IFN gamma , TNF alpha ) production , but was not cytotoxic to immune cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the effect of combinations of humanized 4D5 , and anti p185HER2 MAB , and the cytokines IFN alpha , IFN gamma or TNF alpha on the growth of three p185HER2 positive human abreast carcinoma cell lines , SK BR 3 , BT 474 , and MDA MB 453 . ^^^ All three cell lines were treated with IFN alpha ( 1000 U / ml ) or IFN gamma ( 1000 U / ml ) or TNF alpha ( 100 U / ml ) with or without huMAb 4D5 ( 100 microM ) , and cell growth was determined on days 3 7 . ^^^ While IFN alpha , IFN gamma , TNF alpha , and huMAb 4D5 all inhibited growth , an apparently additive inhibitory effect occurred using combinations of huMAb 4D5 plus cytokine , with huMAb 4D5 plus TNF alpha causing the greatest growth inhibition . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , IL 2 , IL 3 , IL 4 , interferon gamma ( IFN gamma ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and tumor necrosis factor alpha ( TNF alpha ) were not detected in any sample . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma primed macrophages for enhanced tumor necrosis factor ( TNF ) secretion , but inhibited IL 10 production , whereas granulocyte / macrophage colony stimulating factor ( CSF ) , macrophage CSF and also IL 4 enhanced macrophage IL 10 responses after ingestion of Listeria in vitro . ^^^ Finally , monoclonal antibody neutralization of IFN gamma during infection of SCID mice with Listeria inhibited TNF alpha mRNA , but augmented expression of IL 10 mRNA in infected tissues . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interestingly , we found that TNF alpha and IFN gamma were synergistic in the activation of IDO in glioblastoma cells 87HG31 , 86HG39 and U373MG and in native astrocytes . ^^^ This was shown by the measurement of enzyme activity as well as by the detection of IDO mRNA in TNF alpha + IFN gamma activated cells . ^^^ Antibodies directed against TNF alpha or IFN gamma were able to inhibit IDO activity as well as the induction of toxoplasmostasis in glioblastoma cells stimulated with both cytokines . ^^^ We therefore conclude that both TNF alpha and IFN gamma may be involved in the defense against cerebral toxoplasmosis by inducing IDO activity as an antiparasitic effector mechanism in brain cells . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The induction of NK cell activity by HHV 6 was abrogated by monoclonal antibodies ( mAbs ) to IL 15 but not by mAbs to other cytokines ( IFN alpha , IFN gamma , TNF alpha , TNF beta , IL 2 , IL 12 ) suggesting that IL 15 secreted in response to viral infection was responsible for the observed effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of membrane and fluid phase regulators is enhanced by either IFN gamma or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have found that IL 2 , IL 4 , TNF alpha , and IFN gamma are expressed by the time the fourth dose of STZ is given . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation with 250 1 , 000 U / ml of tumor necrosis factor alpha ( TNF alpha ) or interferon gamma ( IFN gamma ) induced both cell types to produce RANTES . ^^^ Used combination , TNF alpha , and IFN gamma synergistically increased mRNA and protein levels . ^^^ Although IFN gamma alone did not induce IL 8 production , it enhanced the effect of TNF alpha on both cell types . ^^^ Keratinocyte production of RANTES and IL 8 , under the influence of cytokines such as TNF alpha or IFN gamma , provides a mechanism for the selective accumulation of leukocytes into immunoinflammatory diseases of the skin and oral mucosa . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There was little spontaneous generation of NO by any of the cells after culture , but synovial fibroblasts , articular chondrocytes and osteoblasts all produced large quantities of NO in response to a cytokine mix of interleukin ( IL ) 1 beta + tumour necrosis factor alpha ( TNF alpha ) + interferon ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The inducible enzyme making NO , inducible NO synthase ( iNOS ) , is transcriptionally activated by IFN gamma and TNF alpha , cytokines which are produced during viral infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Many of the glioblastoma / astrocytoma cell lines were shown to inducibly express intercellular adhesion molecule 1 ( ICAM 1 , CD 54 ) and vascular cell adhesion molecule 1 ( VCAM 1 ) following stimulation with interleukin 1 alpha ( IL 1 alpha ) , interleukin 1 beta ( IL 1 beta ) , tumour necrosis factor alpha ( TNF alpha ) , tumour necrosis factor beta ( TNF beta ) , and interferon gamma ( IFN gamma ) , but not with any of the several other cytokines tested . ^^^ The cell line U 138MG , a human glioblastoma derived line , was the most sensitive one to IL 1 alpha / beta , TNF alpha / beta and IFN gamma for ICAM 1 expression , comparing well with proinflammatory cytokine induced ICAM 1 expression in the endothelial cell hybrid EA hy 926 line , and was shown to be useful for the functional assay of the biological potencies of these individual cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CYP content and several CYP protein levels were assessed in hepatocytes treated with a cytokine combination consisting of tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 beta ( IL 1 beta ) , and interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In Rel / splenic T cell cultures stimulated with phorbol 12 myristate 13 acetate and ionomycin , the levels of IL 3 , IL 5 , granulocyte macrophage colony stimulating factor ( GM CSF ) , tumor necrosis factor alpha ( TNF alpha ) , and gamma interferon ( IFN gamma ) were only 2 to 3 fold lower compared with normal T cells . ^^^ Exogenous IL 2 , which restitutes the proliferative response of the anti CD 3 and anti CD 28 treated Rel / T cells , restores production of IL 5 , TNF alpha , and IFN gamma , but not IL 3 and GM CSF expression to approximately normal levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , TGF beta 1 suppressed secretion of IL 2 , IFN alpha , IFN gamma , TNF alpha but not of IL 1 alpha and IL 1 beta in whole blood cell cultures from healthy individuals . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Anti CD 3 stimulated cultures containing IVIG exhibited a significant inhibition of production of T cell derived lymphokines IL 2 , IL 10 , TNF beta , IFN gamma and TNF alpha ( made by both monocytes and T cells ) , while synthesis of the monokine IL 8 was significantly increased . ^^^ In contrast , no effects were found on IFN gamma or TNF alpha production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The inhibitory effects of IFN gamma , however , were not affected by the presence of TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Activation with LPS and IFN gamma increased the proportion of adult astrocytes expressing detectable TNF alpha and IL 6 mRNA signals ; however , the proportion was significantly less than for microglia contained in the same cultures . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Antigenic stimulation on thymic stromal cells produced large amounts of interferon gamma ( IFN gamma ) and small amounts of tumor necrosis factor alpha ( TNF alpha ) . ^^^ These results indicate that elimination of TCR stimulated T cells on thymic stromal monolayers with the capacity to support thymocyte differentiation is induced by the cross talk between IFN gamma / TNF alpha producing T cells and stromal cells capable of producing NO in response to these lymphokines . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have recently demonstrated that the cross linking of CD 4 molecules ( CD4XL ) results in death of normal peripheral T cells through apoptosis and imbalanced cytokine secretion ( ie , induction of tumor necrosis factor alpha [ TNF alpha ] and interferon gamma [ IFN gamma ] in the absence of interleukin 2 [ IL 2 ] or IL 4 secretion ) . ^^^ These upregulated cytokines ( TNF alpha / IFN gamma ) largely contributed to upregulation of the apoptosis inducing cell surface molecule , Fas ( APO 1 / CD95 ) and apoptosis induction . ^^^ This apoptosis inhibitory effect of vesnarinone was associated with the blocking of CD4XL induced TNF alpha IFN gamma secretion and of Fas antigen upregulation . ^^^ However , vesnarinone did not block effects of exogenously supplemented TNF alpha / IFN gamma on Fas induction . ^^^ These data suggest that vesnarinone inhibits CD4XL induced TNF alpha / IFN gamma secretion , thereby blocking subsequent Fas upregulation and apoptosis induction . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Basal expression of ICAM 1 molecules was enhanced by TNF alpha and to a lesser extent by IFN beta , but was not affected by IFN gamma . ^^^ MHC class 1 expression on ECs was enhanced by IFN beta , IFN gamma , and tumor necrosis factor ( TNF ) alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To determine the immunoregulatory capacity of the phosphodiesterase inhibitor pentoxifylline ( PTX ) , which is known to suppress the production of tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) , this drug was used in mitogen and antigen stimulated lymphocyte cultures as well as in patients with multiple sclerosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study , we have determined the kinetics of constitutive expression of a panel of cytokines [ interleukin ( IL ) 2 , IL 4 , IL 6 , IL 10 , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) ] in sequential peripheral blood mononuclear cell samples from nine individuals with primary human immunodeficiency virus infection . ^^^ Similar to IL 10 and TNF alpha , IFN gamma expression was detected in all patients ; however , in five of nine patients , IFN gamma expression peaked very early during primary infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast to UCHT 1 , scUCHT 1 did not induce T cell proliferation and cytokine release ( TNF alpha and IFN gamma ) in in vitro assays . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , while elevation in the expression of mRNA for TNF alpha and IFN gamma is evident within 24 h after the chemotherapy , elevation in the expression of mRNA for IL 12 ( p 40 ) is first evident 72 h after the chemotherapy . ^^^ Moreover , neutralizing anti IFN gamma mAb , like neutralizing anti TNF mAb but not neutralizing anti IL 12 mAb , reduced the curative effectiveness of low dose melphalan for MOPC 315 tumor bearers . ^^^ Studies into the mechanism through which IFN gamma mediates its antitumor effect in low dose melphalan treated MOPC 315 tumor bearing mice revealed that MOPC 315 tumor cells , which are not sensitive to the direct antitumor effects of TNF , display some sensitivity to the antiproliferative activity of high concentrations of IFN gamma . ^^^ However , unlike TNF alpha , IFN gamma is unable to promote the generation of anti MOPC 315 cytotoxic T lymphocyte activity and , in fact , exerts an inhibitory activity on CTL generation . ^^^ Taken together , our studies illustrate that low dose melphalan therapy of MOPC 315 tumor bearers is associated with the rapid elevation in the expression of mRNA for IFN gamma and TNF , two cytokines which are important for the curative effectiveness of low dose melphalan , and which mediate their antitumor effect , in part , through distinct mechanisms . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , incubation of BAECs with TNF alpha or LPS strikingly enhanced L arginine uptake ( 2 . 5 fold ) , whereas IL 1 beta and IFN gamma had no effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the effects of proximal modulators of cytokines , tyrosine kinase ( TK ) , and protein kinase C ( PKC ) on reactive oxygen species ( ROS ) generation and the induction of scavenging enzymes , superoxide dismutase ( SOD ) , catalase , and glutathione peroxidase ( GSH Px ) of human neutrophils and lymphocytes , by using IL 1 alpha , TNF alpha , and IFN gamma and neutralizing antibodies to these cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Most attention has concentrated on interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) because these cytokines have been shown to limit other protozoan infections . ^^^ Mechanisms by which IFN gamma and TNF alpha modulate parasite reproduction have not been identified . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Northern blot hybridization studies showed that interferons ( IFN alpha , IFN beta , IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) modestly enhanced steady state levels of HLA G mRNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Co incubation of M 07e with various cytokines and TPO showed additive proliferative effects for interleukin 3 ( IL 3 ) and granulocyte macrophage colony stimulating factor ( GM CSF ) and synergistic responses for stem cell factor ( SCF ) , interferon ( IFN ) alpha , and to a lesser extent for IFN gamma and tumor necrosis factor ( TNF ) alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1 beta IL 2 , IL 6 , IL 8 , TNF alpha , TNF beta and IFN gamma , but not IL 1 alpha , were detected in the monkey using human reagents . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We showed that all toxins tested induced IL 1 alpha and beta , IL 2 , IL 4 , IL 6 , IFN gamma , and TNF alpha , but not IFN alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Leukaemic PBMC could function as accessory cells during mitogenic T cell activation resulting in both T cell proliferation and a broad T cell cytokine response [ IL 3 , IL 4 , IL 10 , granulocyte / macrophage colony stimulating factor ( GM CSF ) tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) secretion ] . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor cells from five freshly isolated ovarian tumors and four established human ovarian carcinoma cell lines were analyzed for the production of the immunoinhibitory cytokine transforming growth factor beta ( TGF beta ) before and after exposure to gamma irradiation and / or the cytokines TNF alpha plus IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Their cytokine profiles , as deduced from gamma interferon ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and interleukin 4 ( IL 4 ) and IL 6 production , were of the Th 0 and Th 1 like phenotypes . ^^^ Supernatants from IE 1 specific clones producing IFN gamma and TNF alpha were shown to inhibit CMV replication in U 373 MG cells . ^^^ This effect was due , as found by using cytokine specific neutralizing antibodies , mostly to IFN gamma , which was secreted at higher levels than TNF alpha . ^^^ To better assess the anti CMV activity of cytokines , recombinant IFN gamma and TNF alpha were used and shown to have a synergistic effect on the inhibition of CMV replication and protein expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have recently shown that HBV specific CTL can abolish HBV replication noncytopathically in the liver of transgenic mice by secreting tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) after antigen recognition . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A significant decline in IL 1beta concentrations was shown during the 4 week period , but none in TNF alpha or IFN gamma concentrations was noted . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Regulation of mRNA levels of TNF alpha and the alpha chain of the high affinity receptor for IgE in mast cells by IFN gamma and alpha / beta . ^^^ Rat mast cell lines ( hybrid rat mast cells , HRMC , and rat cultured mast cells , RCMC ) and mast cells from the rat body cavity were used to test the hypothesis that IFN alpha / beta and IFN gamma inhibit tumor necrosis factor alpha ( TNF alpha ) mediated cytotoxicity by depressing the steady state levels of mRNA for TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The percentage of tumor samples in which > or =3 % of the lymphocytes were producing cytokines was as follows : interleukin ( IL ) 2 45 % , IL 4 36 % , tumor necrosis factor alpha ( TNF alpha ) 28 % , transforming growth factor beta 1 ( TGF beta 1 ) 20 % , IL 10 11 % , interferon gamma ( IFN gamma ) 4 % , and granulocyte macrophage colony stimulating factor ( GM CSF ) 3 % . ^^^ Significantly more tumor samples contained lymphocytes producing IL 2 , IL 4 , TGF beta 1 , and TNF alpha than IFN gamma and GM CSF ( p < 0 . 002 for each comparison ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These experiments evaluate blocking endogenous interferon gamma ( IFN gamma ) activity to abrogate TNF alpha toxicity . ^^^ METHODS : C57B1 / 6 mice bearing MCA 105 tumor were treated with TNF alpha and anti IFN gamma antibody ( Ab ) to evaluate the effect on the acute lethality of TNF alpha and their efficacy as evaluated by tumor growth rate , tumor histology , and survival . ^^^ RESULTS : Anti IFN gamma Ab decreased TNF alpha lethality . ^^^ Anti IFN gamma Ab abrogated the antitumor effect of TNF alpha , prevented acute tumor necrosis histologically , and resulted in tumor growth rate and host survival similar to that of controls . ^^^ The findings in mice that received anti IFN gamma Ab and high dose TNF alpha were comparable with those in mice that received a lower , equitoxic dose of TNF alpha alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : LNCaP , DU 145 , and PC 3 cell lines were treated with tumor necrosis factor alpha ( TNF alpha ) ( 200 U / mL ) , interferon gamma ( IFN gamma ) ( 500 U / mL ) , human leukocyte interferon ( IFN alpha ) ( 500 U / mL ) , and interleukin 2 ( IL 2 ) ( 400 U / mL ) . ^^^ Up regulated P cadherin , but not E cadherin , mRNA expression was detected in TNF alpha and IFN gamma treated PC 3 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The anti TNF alpha ribozyme treatment in vivo resulted in a more significant reduction of LPS induced IFN gamma protein secretion compared to IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The resulting CD4+ T cell clones generated under these different experimental conditions were then analyzed for their ability to produce IL 2 , IL 4 , IL 5 , IL 10 , IFN gamma and tumor necrosis factor ( TNF ) beta in response to stimulation with phorbol 12 myristate 13 acetate ( PMA ) + anti CD 3 monoclonal antibody or PMA + ionomycin . ^^^ Different from T cell clones generated from peripheral blood , virtually all CD4+ T cell clones generated from human thymocytes produced high concentrations of IL 2 , IL 4 and IL 5 , but no IFN gamma , TNF beta or IL 10 . ^^^ However , upon addition of exogenous IL 12 , the resulting CD4+ thymocyte clones produced TNF beta , IFN gamma , and IL 10 in addition to IL 4 and IL 5 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The incidence of cytokine ( IFN gamma , IL 6 and TNF alpha ) mRNA and cytokine protein expressing cells was very similar and congruent after both these Shigella derived stimuli . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Incubation of NK cells in the presence of mIgG up regulated expression of surface activation markers ( CD 69 , IL 2R alpha , ICAM 1 ) , cytotoxicity , cytokine production ( IL 1 beta , IFN gamma and TNF alpha ) and release of soluble IL 2R . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Addition of IFN gamma up regulated the expression of the iNOS gene in cells activated by LPS + TNF alpha , but significantly suppressed the induction of IL 1 beta gene expression in a dose dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The response of both atopic and normal subjects to recall bacterial antigens was predominantly Th 1 , with high levels of IFN gamma , IL 2 and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of B 7 1 and B 7 2 on purified B cells were not altered by any of the cytokines tested , including IL 1 alpha , IL 1 beta , IL 2 , IL 4 , IL 5 , IL 6 , IL 10 , IL 12 , IFN gamma , TNF alpha , TGF alpha and GM CSF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
B lymphoid potential was not supported , however , in SF + IL 3 or in SF + IL 3 plus any single growth factor ( IL 1 to 12 , granulocyte macrophage colony stimulating factor [ GM CSF ] , G CSF , erythropoietin [ Epo ] , leukemia inhibitory factor [ LIF ] , tumor necrosis factor alpha [ TNF alpha ] , transforming growth factor beta [ TGF beta ] , gamma interferon [ IFN gamma ] , or insulin like growth factor 1 [ IGF 1 ] ) , but was supported in SF + IL 3 + 5 % PWM SCM . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Thus , bacterial DNA induces in vivo release of IFN gamma which , in turn , is associated with an increase in LPS induced TNF alpha and IL 6 release , and with increased sensitivity to the toxic effects of LPS . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN alphabeta , TNF , IL 12 , and IFN gamma were all shown to be induced 2 days after infection of immunocompetent mice . ^^^ Infections of NK and T cell deficient mice demonstrated that virus induced IFN alphabeta , TNF , and IL 12 , but not IFN gamma , were produced independently of these populations , and that IL 12 production occurred in the absence of detectable IFN gamma . ^^^ Examination of NK cell cytotoxicity , blastogenesis , and IFN gamma production demonstrated that : IL 12 was required for NK cell IFN gamma production but not blastogenesis and cytotoxicity ; IFN alphabeta was necessary for NK cell blastogenesis and cytotoxicity but not IFN gamma production ; and TNF facilitated IFN gamma production but inhibited NK cell cytotoxicity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Spleen and LN levels of IL 6 , IL 10 , TNF alpha , or TGF beta mRNA showed much less up regulation than IL 2 or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There was significantly increased Con A induced secretion of tumor necrosis factor ( TNF ) alpha , IFN gamma , interleukin ( IL ) 2 , IL 6 , and IL 10 and decreased IL 4 secretion in on treatment compared with pretreatment peripheral blood mononuclear cell samples . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We compared the tumor necrosis factor ( TNF alpha ) , interferon gamma ( IFN gamma ) and granulocyte macrophage colony stimulating factor ( GM CSF ) serum levels in 87 patients with malaria from the Brazilian Amazon . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , the AMs from chronic alcohol fed group secreted 42 53 % less TNF alpha spontaneously and in response to LPS , interferon gamma ( IFN gamma ) or IFN gamma + LPS compared with the AMs from pair fed group . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Synovial tissues and spleens were obtained at varying time intervals after bacterial inoculation , and examined for mRNA expression of interleukin 1beta ( IL 1beta ) , IL 4 , IL 10 , IL 12 , tumor necrosis factor alpha ( TNFalpha ) , TNFbeta , interferon gamma ( IFN gamma ) , transforming growth factor beta , and perforin , by an in situ hybridization technique . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Attempts at improving primary T cell activation revealed that exogenous cytokines , including interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) and interleukin 2 ( IL 2 ) , were not effective . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In conclusion , the described similarity shows that allergens and irritants can induce the same profile of IL 1alpha , TNF alpha , IL 2 , and IFN gamma production , resulting in the near impossibility of discriminating between allergic and irritant contact dermatitis at the time of patch test reading . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
As a correlation to this observation in patients , induced IL 2 mRNA levels in unmodified rejection were sequentially measured in PBMC cultures in autologous vs allogeneic canine renal transplants and IL 2 , IL 10 , TNF alpha , and IFN gamma mRNA were also measured in kidney biopsies . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We detected the up regulation of local cytokine genes ( IL 1 beta , TNF alpha , IL 2 , IFN gamma , IL 10 , IL 12p40 ) in the lacrimal glands with very early inflammatory lesions by reverse transcriptase ( RT ) PCR analysis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , when the secretory properties of IFN gamma plus LPS treated BV 2 cells were assessed , we found that tumor necrosis factor remains unchanged while nitric oxide production is significantly reduced in iron loaded cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Secretion of TNF alpha or IL 1 beta , cytokines which themselves increase IL 8 secretion , was not induced by rotavirus , nor was that of TNF alpha , IFN alpha , IFN gamma , or IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
During a later phase , between 4 and 6 months , there is a characteristic TH 1 cytokine profile with production of IL 2 and IFN gamma occurring after stimulation , as well as lymphocytes producing TNF , supposedly TNF beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin ( IL ) 1 beta , but neither IL 6 , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , nor LPS stimulated NO formation in a dose dependent manner and induced half maximal effects at 30 pmol / L . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , neutralizing anti IFN gamma antibodies used alone at 50 micrograms / ml or in combination with anti tumour necrosis factor ( TNF alpha ) , and the L arginine analogue and NO synthase inhibitor , NG monomethyl L arginine monoacetate ( MLA ) , could significantly suppress the ability of protective clones to inhibit MoPn in epithelial cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Effect of human cytokines ( IFN gamma , TNF alpha , IL 1 beta , IL 4 ) on porcine endothelial cells : induction of MHC and adhesion molecules and functional significance of these changes . ^^^ Previous studies using cultured human endothelial cells have demonstrated the role of inflammatory cytokines [ interferon gamma ( IFN gamma ) , tumour necrosis factor gamma ( TNF alpha ) , interleukin 1 beta ( IL 1 beta ) and IL 4 ] in the regulation of major histocompatibility complex ( MHC ) and adhesion molecule expression . ^^^ In view of the likely event of grafted porcine tissues being exposed to human cytokines , we have investigated the effect of IFN gamma , TNF alpha , IL 1 beta , IL 4 and recombinant porcine IFN gamma ( rpoIFN gamma ) on cultured porcine aortic endothelial cells ( PAEC ) with respect to induction / up regulation of porcine MHC and adhesion molecules and B 7 receptors . ^^^ Porcine IFN gamma also up regulated SLA class 1 and class 2 , the ligand for CTLA 4 immunoglobulin and VCAM expression ; the magnitude and kinetics of this response differed to that produced by recombinant human TNF alpha ( rhTNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the genetic control of IFN gamma release during MLR and its relationship with TNF alpha and IL 12 . ^^^ Blocking experiments demonstrated the IFN gamma dependence of TNF alpha production and the significant contribution of IL 12 to IFN gamma secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Levels of IL 4 , IL 5 , TNF alpha , and IFN gamma were quantitated in the intestinal ( afferent ) and efferent thoracic duct lymph of rats during the course ( 0 to 289 h ) of an infection with Trichinella spiralis . ^^^ However , IFN gamma and TNF alpha levels were lower in efferent lymph compared with intestinal lymph suggesting mesenteric node consumption . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of both these cell types with the proinflammatory cytokines interferon gamma ( IFN gamma ) or tumour necrosis factor alpha ( TNF alpha ) significantly upregulated expression of intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study the effect von N acetylcysteine ( NAC ) on the release of interleukin 1 alpha and beta ( IL 1 ) , interleukin 2 ( IL 2 ) , interferon gamma ( IFN gamma ) and tumornecrosisfactor alpha ( TNF alpha ) was assessed in an in vitro assay . ^^^ IFN gamma and TNF alpha : Until 12 mmol / l NAC no changes were observed . 24 mmol / l NAC resulted in a significant decrease , too . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Striking increases in the plasma levels of various cytokines , including tumor necrosis factor ( TNF ) , interleukin 2 ( IL 2 ) , and IFN gamma , were detected before the increase in plasma aminotransferase levels induced by Con A injection . ^^^ TNF levels peaked within 2 hours , whereas IFN gamma levels peaked at 6 hours after Con A injection . ^^^ In contrast to a sharp peak of TNF levels , high IFN gamma levels were detected for a more prolonged period . ^^^ The protection from Con A induced hepatitis was also induced by administration of rIL 6 before Con A injection . rIL 6 treatment induced significant albeit incomplete inhibition of IFN gamma and TNF production , whereas this regimen did not affect IL 2 production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma and IL 4 increased lipopolysaccharide ( LPS ) induced TNF alpha , IL 1 steady state message levels . ^^^ In contrast to IL 1 messages , whose degradation was not significantly affected by either lymphokine , the stability of TNF alpha messages differed after IFN gamma and IL 4 treatment . ^^^ Although IL 4 treatment increased the TNF alpha transcription rate , an increase in the degradation rate of TNF alpha mRNA in the IL 4 treated cells resulted in a lower level of steady state mRNA than in the IFN gamma treated cells . ^^^ Although the binding activity of this factor was not affected by either IFN gamma or IL 4 , the binding of the factor to AU rich sequences appeared to be important in the rapid degradation of TNF alpha messages . ^^^ Thus IFN gamma and IL 4 may differentially affect the post transcriptional control of TNF alpha gene expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have previously shown that pretreatment of LN HEV cells with pro inflammatory cytokines such as tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) , augments their adhesiveness for thoracic duct lymphocytes ( TDL ) . ^^^ The expression of 3C10 antigen on LN HEV cells was increased by incubation with TNF alpha or IFN gamma . ^^^ In addition , TGF beta 1 also abrogated the adhesiveness of LN HEV cells stimulated with TNF alpha , IFN gamma or both cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Epithelial cells obtained from tracheas of donor lungs were stimulated with interleukin 1 beta ( IL 1 beta ) , tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) or with a mixture of the three cytokines ( ' cytomix ' ) . ^^^ Each individual cytokine produced a small increase in RANTES protein : IL 1 beta 24 + / 1 pM , TNF alpha 13 + / 7 pM and IFN gamma 29 + / 7 pM , but cytomix increased protein to 236 + / 22 pM ( P < 0 . 002 ) and mRNA expression by > 20 fold ( P < 0 . 002 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Recombinant human tumor necrosis factor alpha ( TNF alpha ) alone had no effect but acted synergistically with IFN gamma , leading to a two fold increase of insulin binding . ^^^ Our results show that IFN gamma treatment alone or together with TNF alpha , can alter the insulin binding to K 562 cells without changing the expression or affinity of the IR . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Finally , tumor necrosis factor alpha ( TNF alpha produced by 2C11 12P cells was found to be implicated in the hyperproduction of IFN gamma , since addition of neutralizing anti TNF alpha antibodies to cocultures reduced the level of suppression and concomitantly abrogated the hyperproduction of IFN gamma . ^^^ Collectively , our findings indicate that T . brucei elicited suppressive 2C11 12 macrophage cells differentially influence T cell subpopulations : ( 1 ) CD8+ cells are signaled via cell cell contact to produce IFN gamma , and TNF alpha is implicated in this process , and ( 2 ) locally produced IFN gamma and macrophage released factors act in concert to inhibit CD4+ and CD8+ T cell proliferative responses . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These cells were treated with various concentrations of tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , interleukin 1 beta ( IL 1 beta ) , and transforming growth factor beta 1 ( TGF beta 1 ) , alone and in combination . ^^^ In BHP cell lines , IFN gamma , IL 1 beta , and TGF beta 1 inhibited [ 3H ] thymidine incorporation and decreased cell number , but TNF alpha stimulated [ 3H ] thymidine incorporation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The mechanism for the resistance of the CD 28 ( / ) mice was a complete abrogation of TNF alpha accumulation in the serum and a nearly complete ( 90 % ) impairment of IFN gamma secretion in response to TSST 1 injection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
None of the tested cytokines ( interleukin 1 beta [ IL 1 beta ] , IL 4 , IL 6 , IL 10 , tumor necrosis factor alpha [ TNF alpha ] , transforming growth factor beta 2 [ TGF beta 2 ] , interferon gamma [ IFN gamma ] , macrophage colony stimulating factor [ M CSF ] , granulocyte colony stimulating factor [ G CSF ] , and granulocyte macrophage colony stimulating factor [ GM CSF ] ) was capable of enhancing the spontaneous rate of apoptosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Nevertheless , many ongoing alloimmune responses are detectable more than 60 d post transplant in these mice : ( 1 ) continuous inflammatory endothelial activation and low grade cellular infiltration in the graft , ( 2 ) modest frequencies of donor reactive T cells in the graft and spleen , ( 3 ) mRNA for various cytokines ( IL 1 , IL 2 , IL 4 , IL 6 , IFN gamma , TNF alpha , TGF beta ) in the graft , ( 4 ) high levels of donor reactive alloantibody in the circulation , ( 5 ) variable development of allogeneic chimerism in the recipient , and ( 6 ) development of interstitial fibrosis and neointimal hyperplasia in the graft . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inducible nitric oxide synthase activity and expression in a human colonic epithelial cell line , HT 29 . 1 We have determined which cytokines regulate the expression of human inducible nitric oxide synthase ( iNOS ) mRNA and nitrite generation in the human colonic epithelial cell line HT 29 . 2 Growth arrested cell cultures were stimulated with the human recombinant cytokines interleukin 1 alpha ( IL 1 alpha ) , tumour necrosisfactor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) or vehicle added alone or in combination . ^^^ These data suggest that the IL 1 alpha / IFN gamma induced nitrite production by HT 29 cells is dependent on de novo protein synthesis , probably the iNOS enzyme . 8 The addition of TNF alpha produced a significant ( P < 0 . 001 ) 3 fold increase of IL 1 alpha / IFN gamma induced nitrite generation . ^^^ In marked contrast the presence of TNF alpha had no effect on IL 1 alpha / IFN gamma induced iNOS mRNA expression by HT 29 cells . ^^^ These findings suggest that the up regulation by TNF alpha of IL 1 alpha / IFN gamma induced nitrite generation is at the post transcriptional level . 9 These data suggest that pro inflammatory cytokines induce NO production in colonic epithelial cells probably due to the induction of iNOS and these cells may be a major source of NO generation in inflammatory bowel disease . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Finally , when the effector cells were pretreated with both TNF and IFN gamma ( 100 U / ml of each , 16h ) the ADCC activity under sera from UC patients was higher than when either TNF or IFN gamma were used alone . ^^^ These results suggest that TNF and IFN gamma , by increasing ADCC activity in UC lesions , are involved in cell injury in the colonic epithelium . ^^^ IFN gamma appears to increase ADCC activity by increasing the number of high affinity monocyte Fc gamma RI receptors , while TNF increases ADCC activity by a different mechanism . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Peripheral blood T lymphocytes from healthy donors were pretreated for 30 min at 25 degrees C with PGE 2 ( 10 ( 4 ) to 10 ( 7 ) M ) PGF 1 alpha , IBMX or Forskolin ( 10 ( 4 ) to 10 ( 5 ) M ) and cultured for 7 days in the presence of Pokeweed Mitogen ( PWM ) , PGE 2 , IBMX and Forskolin , but not PGF 1 alpha , significantly increased IL 6 production while inhibited IL 2 , IL 3 , IL 4 , IFN gamma , TNF alpha and GM CSF production . ^^^ The results reported here are compatible with the existence of separate pathways of gene induction for IL 2 , IL 3 , IL 4 , IFN gamma , TNF alpha and GM CSF on the one hand and IL 6 on the other one . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Circulating IFN gamma was completely suppressed , and TNF was reduced by 50 % . ^^^ Recombinant TNF alpha administered to mice treated with concanavalin A and anti IFN gamma antiserum failed to initiate liver injury . ^^^ Similar results were obtained with recombinant IFN gamma in concanavalin A challenged mice under the condition of TNF neutralization . ^^^ CONCLUSIONS : Both TNF as well as IFN gamma are critical mediators of liver injury in concanavalin A treated mice , whereas hepatic DNA fragmentation and liver failure in the D galactosamine models depend only on TNF . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These T cell clones were cytotoxic and secreted TNF and additional cytokines in response to the IFN gamma treated , HLA class 2 positive autologous sarcoma cells . ^^^ TNF , IFN gamma , IL 4 , and supernatants of T cell clones induced HLA DR expression on the sarcoma cells and , thus , were able to enhance Ag presentation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The isolated IEL population contains mRNA for IL 1 alpha , IL 1 beta , IL 1R , IL 1RA , IL 2 , IL 6R , IFN gamma , TGF alpha , TGF beta 1 , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NO production by the co cultured cells was abrogated by adding either anti interferon gamma ( IFN gamma ) or anti tumor necrosis factor alpha ( TNF alpha ) antibody . ^^^ Furthermore , the roles of immune T cells and PPD could be replaced by adding recombinant IFN gamma together with TNF alpha to the macrophage cultures , but neither alone was sufficient to induce NO production by the macrophages . ^^^ Our present data indicate that TNF alpha produced by PPD stimulated macrophages and IFN gamma produced by cell cell interaction of BCG immune T cells and antigen engulfed macrophages together activate the macrophages to produce NO . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The combination of IFN gamma and TNF alpha produced no further response compared with the single agents used alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pretreatment of such target cells with tumor necrosis factor ( TNF ) alpha but not with interferon ( IFN ) alpha or IFN gamma increased susceptibility of these cells to lysis by cytotoxic T lymphocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Short term exposure of ex vivo carcinoma and sarcoma cells to IFN gamma and TNF alpha induced or elevated to detectable levels the surface expression of MHC class 1 , class 2 , and ICAM 1 ( CD 54 ) , but only rarely the B 7 ( CD 80 ) molecules . ^^^ This was indicated by the results obtained with several tumors that expressed indigenously high levels of these molecules but reacted with the allogeneic lymphocytes only or more efficiently after treatment with IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found that although IL 2 , IL 3 , and IL 4 mRNA levels are substantially reduced in anergic T cells , mRNAs for IL 6 , IL 10 , IFN gamma , and TNF alpha are expressed normally . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The MNC from MS patients were significantly more adherent than cells from normal healthy subjects to endothelial monolayers pretreated with 0 . 01 U / ml TNF alpha ( 103 % increase ; P = 0 . 002 ) , 0 . 1 U / ml TNF alpha ( 80 % increase ; P < 0 . 01 ) and 1 . 0 U / ml TNF alpha ( 41 % increase ; P < 0 . 02 ) , and to endothelium pretreated with 10 U / ml IL 1 beta ( 44 % increase ; P < 0 . 05 ) and 100 U / ml interferon gamma ( IFN gamma ) ( 100 % increase ; P < 0 . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Three ovarian cancer cell lines , NIH : OVCAR 3 , 3AO , and AO were used in this study to assess the synergistic effect of TNF alpha , IFN gamma and chemotherapeutic drugs ( epirubicin , carboplatin , fluorouracil ) . ^^^ It was found that NIH : OVCAR 3 and 3AO cells were resistant to the individual treatment with TNF alpha and IFN gamma , while AO cells were sensitive to TNF alpha but not to INF gamma . ^^^ Epirubicin and TNF alpha were the two major cytotoxic agents to NIH : OVCAR 3 . 3AO cells were susceptible to epirubicin , 5 Fu , TNF alpha and IFN gamma , while AO cells were sensitive to TNF and epirubicin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although the majority of fLC were positive for the alpha chain of GM CSF receptor ( GM CSFR ) , the beta chain of GM CSFR was detected only on 15 % of CD1a+ cells . fLC were also positive for IL 1 receptor ( IL 1R ) type 1 , IL 1R type 2 , 75 , 000 molecular weight TNF receptor ( TNFR ) and interferon gamma receptor ( IFN gamma R ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This protective effect was correlated with increased levels of gamma interferon ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) in serum . ^^^ To determine if these cytokines were involved in the protective effects of IL 12 , we treated infected mice with IL 12 alone or in combination with monoclonal antibodies specific for IFN gamma or TNF alpha . ^^^ Together , our data identify an IL 12 mediated mechanism of resistance to T . cruzi , which is dependent on IFN gamma and TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Gamma interferon ( IFN gamma ) production by cells from TNF alpha depleted mice was not significantly different from that of cells from control infected mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation by beta glucan ( 500 microg / mL ) or interferon gamma ( IFN gamma ; 100 U / mL ) alone had a slight effect on AM functions , but when AMs were incubated together with beta glucan and IFN gamma , the production and secretion of some immune mediators , such as nitric oxide , interleukin 1 ( IL 1 ) , IL 6 , and tumor necrosis factor alpha ( TNF alpha ) , were markedly augmented . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunotherapy with interleukin 2 ( IL 2 ) is limited by severe side effects thought to be mediated by the activation of immune effector cells and the induction of secondary cytokines including tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) . ^^^ In this study , in C3H / HeN mice , we tested the ability of IL 10 to inhibit IL 2 induced mononuclear cell and alveolar macrophage activation and IL 2 induced increases in serum TNF alpha and IFN gamma , all of which may contribute to the generation of toxicity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This prompted us to study the influence of PTX on a larger spectrum of cytokines with proinflammatory actions [ TNF alpha , interleukin 6 , ( IL ) 6 , IL 1 beta , IL 8 ] or with implied actions in the TH 1 ( IL 2 , IFN gamma ) / TH2 ( IL 10 ) balance . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Induction of hepatic nitric oxide synthase ( NOS ) by tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 beta ( IL 1 beta ) , interferon gamma ( IFN gamma ) , interleukin 6 ( IL 6 ) , and lipopolysaccharide was assessed as activity and immunoreactive protein . ^^^ NOS induction by TNF alpha was dose dependent from concentrations of 0 . 06 to 60 nM and was increased 2 3 fold by IFN gamma . ^^^ NOS 2 activity was induced by lipopolysaccharide , IL 1 beta , TNF alpha , and IFN gamma but not by IL 6 . ^^^ Activation of the transcription factor nuclear factor kappa B by TNF alpha , IFN gamma , and IL 1 beta was assessed by gel shift analysis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Colon 26 cells pretreated with IFN gamma demonstrated resistance to tumor necrosis factor alpha mediated growth inhibition . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The present study was conducted to determine the extent to which the cytokines tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) are required to protect against primary or secondary murine tularaemia caused by the live vaccine strain of the facultative intracellular bacterium Francisella tularensis . ^^^ It is shown that non immune mice treated with neutralizing monoclonal antibodies ( mAbs ) against TNF alpha and IFN gamma are rendered defenceless against otherwise sublethal intravenous inocula of the bacterium . ^^^ However , the need for TNF alpha and IFN gamma for controlling secondary tularaemia became critical when challenge inocula exceeded 10 ( 6 ) c . f . u . ^^^ Additionally , they show that the need for TNF alpha and IFN gamma to combat secondary infection depends on the size of the challenge inoculum . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pre treatment with proinflammatory agonists , including endotoxin , IFN gamma , or IFN gamma + TNF alpha enhanced both hepatic killing efficiency and net hepatic killing . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The enhancement of NO synthesis was thought to be mediated , in part , through tumor necrosis factor alpha ( TNF alpha ) induction by these agents , since a neutralizing antibody to TNF alpha significantly suppressed NO production in RAW264 . 7 cells stimulated with PSK or OK 432 in combination with IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha was substantially weaker ( IC 50 > 10 ng / ml ) but provided synergy with IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of cytokine mRNA species was determined in liver biopsies from six normal subjects , 18 patients with PBC and 14 patients with hepatitis B e antigen ( HBeAg ) positive CHB using a reverse transcriptase polymerase chain reaction ( RT PCR ) technique . cDNA , obtained by reverse transcription using oligo d ( T ) primers , was amplified by PCR using primers specific for the coding region of seven different cytokines ( IL 1 , IL 2 , IL 4 , IL 5 , IL 6 , interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) ) . ^^^ In PBC , mRNA for IFN gamma was detected in 60 % of biopsies , but no mRNAs for IL 1 , IL 2 , IL 4 , IL 5 , or IL 6 , or for TNF alpha , were detected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Since LAK cells have been demonstrated to release a series of different immunomodulatory cytokines , we evaluated the effect of short term incubation of fibroblasts with different factors , including IL 1 , IL 2 , IL 3 , IL 4 , IL 6 , tumour necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) , on the binding and the lysis mediated by LAK cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokines interleukin 1 beta ( IL 1 beta ) and IL 2 strongly enhanced GM CSF production , while IL 4 , IL 6 , GM CSF , interferon gamma ( IFN gamma ) and tumour necrosis factor ( TNF ) had no effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The role of some cytokines including tumour necrosis factor alpha ( TNF alpha ) , interleukin 1 alpha ( IL 1 alpha ) , interferon gamma ( IFN gamma ) , transforming growth factor beta ( TGF beta ) and interleukin 6 ( IL 6 ) in the generation of immunosuppressive macrophages ( M phi s ) in host spleen cells of Mycobacterium avium complex ( MAC ) infected mice was studied . ^^^ The effects of TNF alpha , IL 1 alpha , IL 6 , and IFN gamma alone or combinations of them upon the acquisition of the suppressor activity by cultured splenic M phi s were studied . ^^^ When normal splenic M phi s were treated with each cytokine for 3 days , TNF alpha , IFN gamma , and IL 1 alpha alone caused a slight elevation of their suppressive activity . ^^^ These findings indicate the important roles of TNF alpha , IFN gamma , and IL 1 alpha in the generation of MAC induced suppressor M phi s . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Active MRI lesions per patient increased from 0 . 08 + / 0 . 08 to 1 . 2 + / 0 . 4 ( p < 0 . 02 ) , number of patients with clinical MRI signs of disease activity from 2 of 12 to 8 to 12 ( P < 0 . 04 ) , lymphocyte IFN gamma production from 3 . 0 + / 0 . 7 to 12 . 4 + / 2 . 2 IU / mL ( p < 0 . 01 ) , lymphocyte tumor necrosis factor alpha production from 5 . 8 + / 0 . 9 to 18 . 9 + / 6 . 3 pg / mL ( p < 0 . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Unlike LPS , activation of smooth muscle cells with either interferon gamma ( IFN gamma , 100 u ml 1 ) , tumour necrosis factor alpha ( TNF alpha , 300 u ml 1 ) or interleukin 1 alpha ( IL 1 alpha , 100 u ml 1 ) failed to stimulate L arginine transport or increase nitrite accumulation . 4 . ^^^ When applied in combination with LPS ( 100 micrograms ml 1 ) both IFN gamma and TNF alpha , but not IL 1 alpha , enhanced the effects observed with LPS alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Anti TNF alpha , but not anti IFN gamma , blocked the inhibitory effects of IL 12 on gene transduction . ^^^ Moreover , TNF alpha , but not IFN gamma , suppressed gene transfer to the same degree as IL 12 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , PTX reduced TNF alpha , IFN gamma and IL 10 production and NF kappa B activation . ^^^ PTX inhibited with similar potency IFN gamma , TNF alpha and cell proliferation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition to inhibiting basal renin expression , IFN gamma potentiated the inhibitory effect of tumor necrosis factor alpha ( TNF alpha ) on renin expression . ^^^ IFN gamma also inhibited basal renin release and potentiated the inhibitory effect of TNF alpha by highly purified populations of decidual stromal cells and decidual macrophages prepared by immunomagnetic separation with beads coupled to an anti human leukocyte antigen ( HLA DR ) antibody that binds macrophages but not stromal cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The human neuroblastoma cell line NB 39 nu expressed mRNA coding for inducible nitric oxide synthase ( iNOS ) following treatment with a combination of interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) . ^^^ Trace levels of iNOS mRNA were detected after treatment with IFN gamma alone , and its mRNA level was synergistically enhanced by simultaneous treatment with TNF alpha . ^^^ Dexamethasone inhibited the induction of iNOS mRNA by IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although IL 12 is stimulated by M . bovis BCG in immunocompetent mice , studies with IFN gamma receptor deficient and tumour necrosis factor alpha ( TNF alpha ) receptor deficient mutant mice suggest that M . bovis BCG induced IL 12 secretion depends on IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using flow cytometry , we have characterized the expression of integrin subunits and other adhesion molecules in bladder and colonic cell lines and studied their modulation by the cytokines tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) . ^^^ We show that although TNF alpha and IFN gamma may modulate integrin expression , these effects are , in the case of some integrin subunits , secondary to cytokine induced changes in cell density . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Antibody neutralization studies indicated that the effect of IL 12 was mediated by IFN gamma without apparent participation of TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , we determined serum levels of various cytokines , such as interleukin ( IL ) 1 beta , tumor necrosis factor ( TNF ) alpha , and gamma interferon ( IFN gamma ) , inasmuch as these cytokines are potent inducers of NO production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Analysis of cytokine patterns in disease showed elevations in TNF alpha and IFN gamma , characteristic of the T helper 1 ( Th 1 ) pathway , implicating Th 1 cells and their cytokines in disease pathogenesis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
All of the expected cytokines such as IL 1 , TNF alpha , IL 4 , IL 5 and IFN gamma were found . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Differential and sometimes contradictory effects have been described for tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) on replication of human immunodeficiency virus type 1 ( HIV 1 ) . ^^^ Exclusive application of IFN gamma only slightly enhanced HIV 1 expression , whereas it synergistically potentiated stimulatory effects of TNF alpha . ^^^ Co ordinate action of TNF alpha and IFN gamma is suggested to represent an important mechanism for disease progression in HIV infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Injection of E . coli lipopolysaccharide ( LPS , 10 mg kg 1 , i . v . ) resulted in increases in the serum levels of tumour necrosis factor alpha ( TNF alpha , maximum 60 min after LPS ) , endothelin 1 , ( ET 1 ; maximum 120 min after LPS ) , and interferon gamma ( IFN gamma , maximum 180 min after LPS ) . 3 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The aim of this study was to compare the amounts of IL 1 alpha , IL 1 beta , IL 4 , IL 6 , IL 8 , IL 10 , IL 12 , TNF alpha , TGF beta 1 and IFN gamma in the synovial fluid of 30 patients with rheumatoid arthritis ( RA ) , 40 patients with osteoarthritis ( OA ) and 13 patients with seronegative spondylarthropathies ( SpA ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Generation of TNF alpha , IFN gamma , IL 6 , IL 4 and IL 10 in mouse serum from trichinellosis : effect of the anti inflammatory compound 4 deoxypyridoxine ( 4 DPD ) . ^^^ In these studies we have detected TNF alpha , IL 6 , IFN gamma , IL 4 and IL 10 in the serum of 10 mice infected with T . spiralis . ^^^ After 15 days of T . spiralis infection , TNF alpha reached a maximum level , while IL 6 was maximal after 7 days , IFN gamma at 20 days and IL 4 at 14 days . ^^^ When the animals were treated with 4 DPD at the reported dosages and infected with T . spiralis the inhibition of TNF alpha and IL 6 , were dose dependent in the first 7 days while IL 4 was reduced only at 400 800 micrograms / bolus . 4 DPD treated mice did not statistically ( P > 0 . 05 ) affect the generation of IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Many studies have been focused on the mechanisms of the immunologic features of aging . this review describes our results of studies performed to determine the influence of age on the capacity to produce interleukin 2 ( IL 2 ) , interferon gamma ( IFN gamma ) , interleukin 4 ( IL 4 ) , interleukin t ( IL 5 ) , interleukin 6 ( IL 6 ) and tumor necrosis factor ( TNF ) . ^^^ Mitogen or antigen stimulated cultures of MNC from aged mice also displayed a significant decrease in IFN gamma and IL 2 production as well as TNF beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro experiments showed that IL 6 production by lymphoid cells was relatively unaffected by CsA and CCA but IL 2 , TNF and IFN gamma were suppressed by CsA . ^^^ The results indicate that CsA and CCA may modify the response to the arthritic adjuvant by specifically inhibiting IL 2 , TNF and IFN gamma production at the time of adjuvant injection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , interferon gamma ( IFN gamma ) and / or tumor necrosis factor alpha ( TNF alpha ) induced the expression of Fas after 48 hours of serum free culture . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To investigate the spectrum of cytokines expressed by peripheral blood mononuclear cells ( PBMC ) from cynomolgus macaques ( Macaca fascicularis ) , we used a semi quantitative RT PCR to determine levels of mRNA coding for IL 1 beta , IL 2 , IL 4 , IL 6 , IL 10 , IFN gamma , and TNF alpha . ^^^ While transcripts or IL 2 , IL 4 and IFN gamma were either low or not detected in unmanipulated PBMCs , varying levels of IL 1 beta , IL 5 , IL 10 , and TNF alpha were readily detected in the same samples . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 4 , IL 10 and IFN gamma have distinct , but interacting , effects on differentiation induced changes in TNF alpha and TNF receptor release by cultured human monocytes . ^^^ We therefore investigated the influence of differentiation over 15 days in vitro on the spontaneous and LPS and IFN gamma induced release of TNF alpha and sTNF R from human monocytes and examined the actions of IL 4 and IL 10 on these . ^^^ LPS stimulated release of TNF alpha declined substantially after the first day and was consistently suppressed by IL 10 and IL 4 but increased by IFN gamma . ^^^ The effects of LPS and IFN gamma on TNF alpha and sTNF R 75 release were progressively lost with increasing time in culture in the presence of IL 4 . sTNF R 55 was not detectable after the first day of culture under any of these conditions . ^^^ IL 4 , IL 10 and IFN gamma therefore have distinct , but interacting , effects on the balance between TNF alpha and sTNF R 75 release by maturing monocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
AMs stimulated with lipopolysaccaride ( LPS ) for four hours in vitro expressed IL 1 alpha , IL 1 beta , IL 6 , TNF alpha and GM CSF ; PBMCs stimulated with LPS for four hours expressed IL 1 alpha , IL 1 beta , IL 6 , TNF alpha and GM CSF and when stimulated with concanavalin A ( ConA ) expressed IL 2 , IL 4 , IFN gamma and GM CSF efficiently . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Likewise , the NOD derived NIT 1 beta cell line did not express surface B 7 or B 7 1 mRNA either constitutively or following exposure to IFN gamma and TNF alpha , two cytokines known to be present in the insulitis lesion of NOD mice , or cAMP which can induce B 7 1 expression on B cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Major histocompatibility ( MHC ) class 1 gene expression is synergistically induced by the cytokines TNF alpha and IFN gamma . ^^^ We demonstrated here that TNF alpha induced binding of NF kappa B p 50 and p 65 to the NF kappa B like element of the MHC class 1 promoter termed region 1 and IFN gamma induced binding of IRF 1 to the adjacent interferon consensus sequence ( ICS ) . ^^^ These results provide a molecular mechanism by which TNF alpha and IFN gamma synergistically induce the expression of a variety of genes involved in immune responses , including MHC class I . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study was undertaken to determine the effects on systemic fibrinolysis of hyperthermic isolated limb perfusion with recombinant tumor necrosis factor alpha ( r TNF alpha ) and melphalan , with or without pretreatment with recombinant IFN gamma ( r IFN gamma ) . ^^^ Of the twenty patients treated with both r TNF alpha and melphalan , eight received r IFN gamma for two days before the perfusion and as a bolus into the perfusion circuit . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have investigated the effects of the two prominent inflammatory cytokines , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) , on oligodendroglial lineage cell development and survival . ^^^ Purified oligodendrocytes and oligodendrocyte precursors obtained from neonatal rat brain primary cultures were subcultured in a defined , serum free medium and exposed to IFN gamma ( 1 100 U / ml , TNF alpha ( 25 100 ng / ml ) or both ( 100 U / ml and 50 ng / ml respectively ) from day 1 to day 3 or from day 3 to day 6 . ^^^ TNF alpha synergized with IFN gamma , but was ineffective by itself . ^^^ Again , the effect of IFN gamma was potentiated by TNF alpha , which was ineffective when tested alone . ^^^ In particular , the message for myelin basic protein ( and , in the case of treatment from days 3 to 6 , also that for myelin associated glycoprotein ) was decreased in cultures exposed to IFN gamma , and further depressed in cultures treated with IFN gamma and TNF alpha , while TNF alpha alone was ineffective . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the absence of endogenous IL 10 , mice acutely infected with Toxoplasma gondii succumb to a lethal immune response dependent on CD4+ T cells and accompanied by overproduction of IL 12 , IFN gamma and TNF alpha . ^^^ Levels of IL 12 and IFN gamma in sera of infected IL 10 deficient animals were four to sixfold higher than those in sera from control mice , as were mRNA levels for IFN gamma , IL 1 beta , TNF alpha , and IL 12 in lung tissue . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We previously demonstrated that TNF alpha , IL 1 beta , IFN gamma , or IFN gamma plus LPS ( IFN gamma / LPS ) can enhance ICAM 1 expression in astrocytes , while microglia express ICAM 1 only in response to IFN gamma or IFN gamma / LPS . ^^^ Similar results were observed in two human astroglioma cell lines , CRT and STT ; TGF beta inhibited TNF alpha and IL 1 beta induced ICAM 1 expression , but IFN gamma induction of ICAM 1 was unaffected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The results have demonstrated that ( 1 ) beta amyloid peptide ( 25 35 ) , in the presence of IFN gamma or TNF alpha , induces the production of NO in the astrocyte cell line C 6 , while neither cytokine was effective per se ; ( 2 ) NO generation is also synergically induced by beta amyloid peptide ( 25 35 ) in the presence of IL 1 beta , the latter being a cytokine able to activate astrocytes per se ; ( 3 ) the effect of beta amyloid peptide ( 25 35 ) is due to the induction of the expression of the gene of inducible NO synthase . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma production could be completely restored by rIL 12 , whereas the addition of IL 1 beta , TNF alpha , or indomethacin had no such effect , nor did the addition of mAb to CD 28 , added to compensate for the reduced B 7 expression of UVB irradiated monocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Freshly isolated IEL at all three gut levels expressed mRNA for IL 1 beta , IL 2 , IL 8 , IFN gamma , and TNF alpha . ^^^ All three jejunal IEL subpopulations ( CD 4 CD8 TCR gamma delta+ , CD4+TCR alpha beta+ , CD8+TCR alpha beta+ ) expressed the same four cytokines , IL 2 , IL 8 , IFN gamma , and TNF alpha , indicating that CD4+TCR alpha beta+ IEL are Th 1 cells and that TCR gamma delta+ IEL and CD8+TCR alpha beta+ IEL include cytotoxic effector cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The requirements for induction of transcription factors reflected the requirements for the secretion of multiple cytokines , including IL 2 , TNF alpha , IFN gamma , and granulocyte macrophage CSF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Ex vivo analysis of cytokines produced in response to the immunizing Ag showed an enhancement in the levels of IFN gamma , TNF alpha , and nitric oxide following IL 4 treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
T cells from protected and nonprotected strains differed in their production of IFN gamma and TNF alpha following immunization with MSP 1 ( 19 ) , but it was unclear how the differential patterns of cytokine expression related to the level of protection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Finally , we provide evidence that the Abeta ( 25 35 ) and Abeta plus IFN gamma induced production of MCP 1 is , in large part , mediated in an autocrine fashion by endogenous TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The production of nitric oxide by macrophages from TNFRp 55 / mice required the presence of the parasites , however , since in their absence TNF could only synergize with IFN gamma for nitric oxide production when added to normal , but not TNFRp 55 / , macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inhibition of platelet endothelial cell adhesion molecule 1 synthesis and leukocyte transmigration in endothelial cells by the combined action of TNF alpha and IFN gamma . ^^^ In this paper , we examined the effects of two inflammatory cytokines , TNF alpha and IFN gamma , on PECAM 1 and vascular endothelial cadherin / catenin organization . ^^^ We found that the addition of inflammatory cytokines ( TNF alpha plus IFN gamma in combination , for > or = 24 h ) caused PECAM 1 to disappear from EC intercellular contacts . ^^^ This phenomenon was only found when TNF alpha plus IFN gamma were used in combination . ^^^ Adhesion of polymorphonuclear cells to doubly treated EC was increased compared with control cells or cells incubated with TNF alpha or IFN gamma separately . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Simultaneous addition of anti CD8O and anti CD 86 Abs also inhibited production of IFN gamma , TNF alpha , and IL 4 , with no effect on IL 10 production , for both allo and autologous T cell proliferation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of various cytokine mRNAs , especially those of interleukin 1 ( IL 1 ) , tumor necrosis factor alpha , gamma interferon ( IFN gamma ) , and IL 12 , was observed to occur in spleen cells after direct stimulation with an LLO preparation purified to a single band on sodium dodecyl sulfate polyacrylamide gel electrophoresis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To test the hypothesis that macrophage derived mediators contribute to the resistance of alveolar macrophages to L . pneumophila , we incubated adherent rat alveolar macrophages with Escherichia coli lipopolysaccharide ( LPS ) , recombinant tumor necrosis factor alpha ( TNF alpha ) , recombinant IFN gamma , neutralizing anti TNF alpha , and / or N ( G ) monomethyl L arginine ( L NMMA ) for 6 h before challenge with L . pneumophila . ^^^ We also measured bioactive TNF alpha release by infected macrophages in the presence or absence of IFN gamma . ^^^ Both LPS and TNF alpha acted synergistically with IFN gamma at less than the maximally activating concentration to suppress L . pneumophila growth . ^^^ Killing of L . pneumophila by IFN gamma at the maximally activating concentration was inhibited by anti TNF alpha . ^^^ The synergistic effects of TNF alpha . or LPS in combination with IFN gamma were inhibited by L NMMA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytometric studies demonstrated that ICAM 1 expression on AH 70 cells increased after addition of IFN gamma , IL 1beta , tumor necrosis factor ( TNF ) alpha or KC , while in hepatocytes ICAM 1 was not increased . ^^^ TNF alpha but not IFN gamma was detected in the supernatant of co culture between KC and AH 70 cells , and this production was partially inhibited by anti ICAM 1 and anti CD 18 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : We injected IL 1 , IL 6 , IFN gamma , TNF alpha with or without D galactosamine ( D GAL ) into the abdominal cavity of mice separately . ^^^ The serum total bilirubin ( TBIL ) and liver necrosis of mice increased more markedly by using of TNF alpha , IL 6 or IFN gamma separately with D GAL ( TBIL : 46 . 2 + / 10 . 6 micromol / L , 44 . 6 + / 12 . 9 micromol / L , 41 . 9 + / 14 . 9 micromol / L ) , then by D GAL alone ( TBIL : 27 + / 11 micromol / L ) also the serum TBIL of mice and liver necrosis also increased after injection of IL 1 , IL 6 with D GAL and TNF alpha . ^^^ CONCLUSION : Cytokines like IL 1 , IL 6 , IFN gamma and TNF alpha joined the process of hepatocyte necrosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Disease exacerbation was associated with enhanced production of the encephalitogenic cytokines TNF alpha , IFN gamma and IL 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Activation of the monocytic cell line THP 1 , or freshly isolated peripheral blood monocytes with IFN gamma , but not IL 3 , GM CSF , IL 6 , or TNF alpha , greatly sensitized the cells toward G 28 5 sFv PE 40 , lowering the EC 50 value from > 10 micrograms / ml to 80 ng / ml . ^^^ This sensitization could not be explained simply by the two to threefold increase in cell surface CD 40 expression induced by IFN gamma since TNF alpha or the combination of TNF alpha and IL 6 gave similar increases in CD 40 expression but did not sensitize the cells to the immunotoxin . ^^^ IFN gamma treated but not untreated THP 1 cells produced IL 6 and TNF alpha following incubation with G 28 5 sFv PE 40 , indicating an association between CD 40 signaling , which induces cytokine production , and sensitivity to the immunotoxin . ^^^ A combination of IFN gamma and TNF alpha , but neither cytokine alone , sensitized the endothelial cells to G 28 5 sFv PE 40 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation of rat pleural mesothelial cells with combinations of interleukin 1 beta ( IL 1 beta ) , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and LPS induced the synthesis of nitric oxide as measured by the oxidation products nitrite ( NO 2 ) and nitrate ( NO 3 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Incubation of islets with interleukin ( IL ) 1 beta ( 1 ng / ml , 24 h ) , tumour necrosis factor alpha ( TNF alpha ; 200 units / ml , 24 h ) or interferon gamma ( IFN gamma ; 500 units / ml , 72 h ) significantly decreased the synthesis of both GAD 65 and GAD 67 , but reduced neither total protein synthesis nor insulin accumulation in the medium or content . ^^^ Inhibition of GAD 65 and GAD 67 protein synthesis by IL 1 beta , TNF alpha or IFN gamma was reversed by co incubation with the nitric oxide synthase inhibitor , NG monomethyl arginine ( NMMA ) . ^^^ Thus the synthesis of both isoforms of islet GAD is selectively decreased in the presence of IL 1 beta , TNF alpha or IFN gamma by a NO mediated mechanism , probably at the level of cytokine gene transcription . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effect of IL 2 could be partially mimicked by IL 4 , IL 10 , or IL 12 , but not by IL 13 , IFN gamma , IL 1 beta , TNF alpha , or by exposure to anti CD 3 , anti CD 28 or phytohemagglutinin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Fas mediated neutrophil apoptosis was suppressed by incubation with G CSF , GM CSF , IFN gamma , TNF alpha , or dexamethasone , as well as the selective tyrosine kinase inhibitors , herbimycin A and genistein . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Thus , expression of NOS 2 is induced by IFN gamma , TNF and IL 1 as well as microbial products whereas IL 4 , IL 10 and TGF beta down regulate its synthesis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In combination , the cytokines , human recombinant interleukin 1 beta ( IL 1 beta ) , human recombinant tumor necrosis factor alpha ( TNF alpha ) , and human recombinant interferon gamma ( IFN gamma ) , induce the time dependent formation of nitrite and prostaglandin E 2 ( PGE 2 ) by human islets . ^^^ This combination of cytokines ( IL 1 beta , TNF alpha , and IFN gamma ) also induces the expression of iNOS mRNA by human islets as demonstrated by both reverse transcriptase polymerase chain reaction and Northern blot analysis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Secretion of interferon gamma ( IFN gamma ) , tumour necrosis alpha ( TNF alpha ) and IL 13 was inhibited by the addition of IFN beta , whereas IL 4 secretion was unaffected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunological characteristics of disease in IFN gamma deficient mice were comparable to those seen in susceptible ( SJL / J ) mice with EAE , including perivascular infiltration in the CNS and order of magnitude increases for both CD 3 gamma chain and TNF alpha mRNA levels in the spinal cord . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Several cytokines , including IFN gamma , IL 1 alpha , IL 6 and TNF alpha have been shown to modulate the expression of adhesion molecules which participate in inflammatory process by recruitment of lymphocytes into inflammatory sites , contributing to the progression of the local inflammatory reaction in chagasic cardiomyopathy . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Preincubation of RAW 264 . 7 cells with herbimycin A or genistein ( but not with either of three tyrphostins tested ) significantly blocked TNF and NOS production on exposure of these cells to combinations of lipopolysaccharide ( LPS ) and interferon gamma ( IFN gamma ) . ^^^ The addition of either genistein or herbimycin A to RAW 264 . 7 cell cultures 1 6 It after stimulation with LPS and IFN gamma had little or no effect on TNF production but markedly inhibited NOS protein accumulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found that ( 1 ) UVB produces a dose dependent inhibition of the proliferative response to stimulators by human BMMCs ; ( 2 ) increasing doses of UVB irradiation and increasing levels of T cell depletion ( TCD ) are both inversely related to production of lymphokines ( IL 2 , IL 3 , LIF , IFN gamma , and GMCSF ) and ( 3 ) T cell depletion , but not UVB irradiation , decreases the production of monokines ( IL 1 , TNF , IL 6 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin 1 beta ( IL 1 beta ) and tumor necrosis factor alpha ( TNF alpha ) had no effect when used alone but enhanced iNOS mRNA upregulation by IFN gamma . iNOS transcription after LPS was present only for 4 h after exposure yet was more sustained after IFN gamma / TNF alpha , LPS was unique in that it transiently induced iNOS mRNA , whereas IFN gamma / TNF alpha resulted in prolonged increases in iNOS mRNA . ^^^ Both LPS and IFN gamma / TNF alpha caused prolonged elevation of immunoreactive protein . ^^^ After IFN gamma or IFN gamma / TNF alpha , iNOS activity declined only moderately . ^^^ LPS added to IFN gamma alone or IFN gamma / TNF alpha did not result in more rapid decay of iNOS enzymatic activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation of the epithelial cells with IFN gamma or the combination of IFN gamma and tumor necrosis factor alpha ( TNF alpha ) ( which doubles the ICAM 1 expression ) increased neutrophil adhesion . ^^^ This effect was even more pronounced with epithelium preincubated with IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
An LFA 1 ICAM 1 mechanism of PMNL migration through HSF and HDF monolayers could be detected after treatment ( 4 hr ) of the monolayers with TNF alpha plus IFN gamma , which upregulated ICAM 1 on the fibroblasts . ^^^ However , in inflammatory conditions in which TNF alpha and / or IFN gamma may be generated , a role for LFA 1 ICAM 1 may be induced . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment with hemin plus IL 2 effectively enhanced the production of TNF alpha and IFN gamma in PBMC . ^^^ CONCLUSION : Hemin upregulated both IL 2 receptor and transferrin receptor expression , and stimulated TNF alpha and IFN gamma production in PBMC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In MAIDS resistant BALB / c mice , cytokine transcripts peaked at 1 week after infection with Type 1 cytokines [ interleukin 2 ( IL 2 ) , tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , IL 12 ] , dominating over Type 2 cytokines ( IL 4 , IL 10 ) . ^^^ Inclusion of anti IL 12 or anti TNF alpha antibodies blocked induction of IFN gamma by LPS . ^^^ Induction of IFN gamma by IL 12 was potentiated by TNF alpha following stimulation of intact spleen cells and purified CD4+ or CD8+ T cells , as well as negatively selected CD 4 8 cells from infected B 6 mice . ^^^ These studies suggest that continuing low level expression of IFN gamma , stimulated by IL 12 and TNF alpha , contributes to the susceptibility of B 6 mice to MAIDS but is not required for the resistance of BALB / c mice to disease . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Total correction of epithelial abnormalities was achieved only by treating T cell conditioned medium with anti interferon gamma ( IFN gamma ) before addition to monocytes , as well as addition of anti tumor necrosis factor alpha ( TNF alpha ) to the coculture . ^^^ Exogenous recombinant IFN gamma and TNF alpha added to T 84 monolayers did not mimic the physiological changes induced by immune cells ; addition of these cytokines to monocytes did reproduce the effects . ^^^ We conclude that T cell derived IFN gamma activates monocytes to release TNF alpha and other soluble mediators , resulting in epithelial dysfunction . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) , in the presence of tumor necrosis factor alpha ( TNF alpha ) , decreases proliferation of a human salivary gland ductal cell line , HSG ( Wu , A . , R . ^^^ We examined the possible effects of these cytokines ( 1 , 000 U / ml IFN gamma + / 20 U / ml TNF alpha for 7 days ) on Ca2+ mobilization in HSG cells . ^^^ Serum induced a proliferative effect on HSG cells , which was suppressed ( > 90 % ) by treatment with IFN gamma + / TNF alpha , but not with TNF alpha alone . ^^^ Serum , carbachol , and thapsigargin stimulated [ Ca2+ ] 1 elevations were reduced by 90 , 60 , and > 65 % , respectively , in cells treated with IFN gamma + / TNF alpha and 30 , 45 , and 45 % , respectively , in cells treated with TNF alpha . ^^^ These data demonstrate that prolonged treatment of HSG cells with IFN gamma + / TNF alpha leads to a persistent depletion of intracellular Ca2+ stores . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) in combination or lipopolysaccharide ( LPS ) alone gave time and dose dependent increases in ICAM 1 . ^^^ Sustained expression of ICAM 1 was observed after short exposure ( 30 min ) to TNF alpha + IFN gamma , but not to LPS . ^^^ Dexamethasone ( 1 , 000 nM ) did not inhibit TNF alpha + IFN gamma induced ICAM 1 expression or mRNA induction . ^^^ Adhesion of unstimulated human neutrophils to EAhy 926 monolayers activated with TNF alpha + IFN gamma or LPS was increased in the presence of dexamethasone at low doses , whereas neutrophil adhesion to LPS but not cytokine stimulated endothelial cells was significantly reduced ( P < 0 . 05 ) in the presence of a high dose of dexamethasone ( 1 , 000 nM ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Semi quantitative RT PCR experiments using eotaxin specific primers indicate low constitutive eotaxin mRNA expression in human dermal fibroblasts which is upregulated by IL 1 alpha and TNF alpha within 6 hrs and modulated by IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Upon incubation with differentiation inducers such as 12 O tetradecanoylphorbol 13 acetate , lipopolysaccharide , a combination of interleukin ( IL ) 6 plus tumor necrosis factor ( TNF ) alpha , or IFN gamma plus TNF alpha , a pronounced increase in the amounts of Met mRNA and protein are seen in THP 1 cells . ^^^ Moreover , we also found that the mRNA for Ron , which encodes a tyrosine kinase receptor for HGF like protein ( also known as macrophage stimulating protein ) , is induced in THP 1 cells during the course of their differentiation to macrophages by IFN gamma plus TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lines of purified HIBEC were activated by culture with the proinflammatory cytokines interferon gamma ( IFN gamma ) and tumor necrosis factor a and were mixed in coculture with allogeneic CD4+ T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we demonstrate that Con A but not tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , or actinomycin D ( ActD ) induced the susceptibility of hepatocytes to activated autologous lymphocyte cytotoxicity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum levels of IL 2 , 4 , 6 , 10 , TNF alpha , and IFN gamma were measured . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Since glutamic acid decarboxylase 65 ( GAD 65 ) is a target autoantigen in IDDM , we investigated whether the cytokines IL 1 beta , TNF alpha IFN gamma altered islet cell expression of GAD 65 and whether the effect of cytokines on GAD 65 expression was similar to their effect on insulin secretion . ^^^ In summary : 1 ) IL 1 beta dramatically inhibits GAD 65 expression . 2 ) TNF alpha and IFN gamma have no effect on GAD 65 expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To define the immunological function of EC adherent T cells from HAM patients , we investigated the degree of spontaneous proliferation and the production of inflammatory cytokines , such as tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) and granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^ Both the degree of spontaneous proliferation and the production of TNF alpha , IFN gamma , and GM CSF by EC adherent T cells of HAM patients were significantly increased , compared to anti HTLV 1 seronegative controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The mRNA levels for IL 4 , IL 10 , and IL 13 were all increased by the pretreatment , whereas synthesis of IFN gamma and TNF alpha was diminished . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To determine the critical cytokines mediating IL Ira expression , oil elicited peritoneal Mphi were exposed to graded doses ( 0 . 1 to 10 ng / ml ) of cytokines ( IL 1beta , IL 2 , IL 4 , IL 10 , IL 12 , IFN gamma , and TNF alpha ) for 24 h , then stimulated with opsonized zymosan . ^^^ Of the cytokines tested , IFN gamma and TNF alpha were the best costimuli for IL 1ra production in the presence of zymosan , whereas IL 1beta , IL 10 , and IL 12 were not active . ^^^ In vivo depletion of IL 4 , IL 10 , IL 12 , IFN gamma , or TNF alpha with 5 mg of cytokine specific neutralizing rabbit IgG revealed that IFN gamma and TNF alpha were required for maximal IL 1ra production by Mphi . ^^^ Our findings indicate that IL 1ra is a common regulatory product of inflammatory Mphi and is particularly promoted by type 1 cytokines , IFN gamma , and TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Previously it was shown that bacterial LPS , TNF alpha , IFN gamma , and cAMP could all inhibit CSF 1 stimulated BMM DNA synthesis and proliferation . ^^^ Using the combined approach of blocking IFN alpha beta Ab and the IFN receptor `` knockout ' ' mice , it was found here that the growth inhibitory effects of LPS and TNF alpha are due , to a significant extent , to endogenous IFN alpha beta , whereas those of IFN gamma and cAMP occur by a different mechanism . it is proposed that the type 1 IFN receptor ( IFNAR 1 ) `` knockout ' ' mice may be useful in delineating some of the in vivo actions of CSF 1 , LPS , TNF alpha , and possibly other agents . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , the kinetic expression of E selectin by human cerebral endothelium was studied under standard conditions and following treatment of primary cultures with bacterial lipopolysaccharide ( LPS ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 1beta ( IL 1beta ) , and interferon gamma ( IFN gamma ) . ^^^ LPS and TNF alpha were most effective followed by IL 1beta , while the IFN gamma had no effect on E selectin expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Astroglia and microglia , the most numerous cells in the central nervous system ( CNS ) , have been shown to produce the inducible nitric oxide synthase ( iNOS ) and tumor necrosis factor alpha ( TNF alpha ) upon stimulation with the cytokines IFN gamma , IL 1 beta , or bacterial lipopolysaccharides ( LPS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This syndrome has been attributed to the transient release of several cytokines including TNF alpha , IFN gamma , IL 2 , IL 3 , IL 6 , and GM CSF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The localization and production at the single cell level of 19 different human cytokines , IL 1 alpha , IL 1 beta , IL 1ra , IL 2 , IL 3 , IL 4 , IL 5 , IL 6 , IL 8 , IL 10 , IL 13 , TNF alpha , TNF beta , IFN gamma , GM CSF , G CSF , and TGF beta 1 3 , were studied in cryopreserved tonsillar tissue using immunohistochemical staining . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NO after treatment with a combination of cytokines ( IL 1 beta , TNF alpha , IFN gamma ) , LPS and zymosan activated serum ( CZ ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Levels of IL 2 , IFN gamma , TNF alpha , IL 10 , and sICAM 1 were measured by enzyme linked immunosorbent assay in sera from 29 of the 104 individuals when not pregnant and 92 of these women when pregnant between 6 and 7 weeks of gestation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To evaluate the utility of a human neuronal cell line , NT2N , for studies of HIV related neuronal cytotoxicity , we studied cellular viability after exposure to HIV 1 gp 120 , tumor necrosis factor alpha ( TFN alpha ) , platelet activating factor ( PAF ) , interleukin 1 beta ( IL 1 beta ) , and interferon gamma ( IFN gamma ) , all of which have been implicated in previous publications as having a role in HIVD ( Brenneman et al , 1988 ; Dreyer et al , 1990 ; Merrill et al , 1992 ; Gelbard et al , 1993 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine treatment ( IL 1 alpha , IL 6 , IFN gamma and TNF alpha ) , did not induce oncogene over expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
HT 29 ( cl 19A ) cells were grown to confluency and incubated with neutrophils under conditions of : ( 1 ) neutrophil stimulation with phorbol myristate acetate ; ( 2 ) monolayer stimulation with interferon gamma , tumour necrosis factor alpha ( IFN gamma , TNF alpha ) ; and ( 3 ) recent epithelial cell trypsinisation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , we quantified the production of IL 2 , TNF alpha and IFN gamma by lymphocytes of alcoholic cirrhosis patients compared to controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Low levels of IL 15 were expressed by unstimulated human fetal astrocytes and microglia , and treatment of astrocytes with interleukin 1 beta ( IL 1 beta ) , interferon gamma ( IFN gamma ) , or tumor necrosis factor alpha ( TNF alpha ) increased the expression of IL 15 at both the mRNA and protein level . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The HTLV 1 infected TCC produced high amounts of IL 1 alpha , IL 3 , IL 6 , IL 8 , TNF alpha , IFN gamma , and GM CSF , and low but significant levels of IL 2 and IL 10 without any stimuli . ^^^ Prostaglandin E 2 depressed the production of IL 1 alpha , while it up regulated the production of IL 6 , TNF alpha , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although type 1 cytokine production ( IFN gamma , IL 2 , and TNF ) was initially induced in TCDD treated mice , production failed to increase normally after day 5 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 10 blocked TNF alpha production by infected cells , but exogenous TNF alpha did not reverse the activating defect in cells cocultured with IFN gamma and IL 10 . ^^^ In summary , IL 10 significantly enhances the growth of L . pneumophila in human monocytes , reverses the protective effect of IFN gamma , blocks TNF alpha secretion , and is secreted by infected monocytes and alveolar macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Amounts of IFN gamma , TNF alpha , IL 6 , and IL 10 mRNA detected by reverse transcriptase PCR did not differ between brains of infected IL 4 / and controls , although brains of the former mice had greater numbers of inflammatory mononuclear cell infiltrates . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The intraperitoneal ( i . p . ) administration of IL 12 , a potent activator of IFN gamma and TNF alpha production , results in a substantial increase in type 3 NOS immunoreactivity in astrocytes . ^^^ Cytokine mediated activation of type 3 NOS is observed in vitro following exposure of a C 6 glioma cells , which constitutively express type 3 NOS , to IL 12 , IFN gamma , and TNF alpha treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found up regulation of local cytokine genes ( IL 1 beta , TNF alpha , IL 2 , IFN gamma , IL 6 , IL 10 , IL 12p40 ) and cell adhesion molecule genes ( ICAM 1 , LFA 1 , CD 44 , Mel 14 ) in the salivary glands from mice with EAS by RT PCR , which were supported by immunohistochemistry . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Skin samples from seven patients with cutaneous chronic GVHD , six post BMT controls and six normal controls were evaluated by reverse transcription polymerase chain reaction for the proinflammatory cytokines interleukin 1 alpha ( IL 1 alpha ) and tumor necrosis factor alpha ( TNF alpha ) , Th 1 associated cytokines IL 2 and interferon gamma ( IFN gamma ) , Th 2 associated cytokines IL 4 , IL 5 and IL 10 , and fibrosis associated cytokines platelet derived growth factor ( PDGF ) and transforming growth factor beta ( TGF beta ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study we utilized a RNase protection assay to quantitatively and simultaneously examine the expression of 10 different cytokine genes , namely IL 1 alpha , 2 1 beta , IL 2 , IL 3 , IL 4 , IL 5 , IL 6 , IFN gamma , TNF alpha , and TNF beta in kidney , spleen , liver , and lymph nodes obtained from pre diseased and diseased lupus prone MRL / lpr , pre diseased MRL / +2 and C57BL / 6 lpr mice , as well as healthy non autoimmune C57BL / 6 and Balb / c mice . ^^^ Increased message for both TNF alpha and IFN gamma genes was also observed in lymph nodes , and less consistently , in the spleen , and kidneys derived from diseased MRL / lpr mice as compared to pre diseased MRL / +2 or normal nonautoimmune control mice . ^^^ In summary , these results demonstrate that generalized up regulation of IL 1 beta gene expression , in concert with a more limited up regulation of both TNF alpha and IFN gamma expression , are prominent features of the autoimmune syndrome in the MRL / lpr model of SLE and may contribute to the disease accelerating effect of the lpr mutation . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TEC constitutively express abundant cell surface CD 44 that is modestly up regulated in response to 18 hours stimulation with TNF alpha ( 100 ng / ml ) , IFN gamma ( 100 U / ml ) and IL 1 ( 100 ng / ml ) . ^^^ Stimulation with IFN gamma or TNF alpha for six hours markedly up regulates CD 44 mRNA expression in these cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The role of Th 1 mediated cytokins such as IL 2 , IFN gamma and TNF beta in the host defence mechanism against mycobacterial infection was extensively discussed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha stimulation increased IL 1 alpha production by both cell types and exhibited synergy with interferon gamma ( IFN gamma ) , although the latter had no effect by itself . ^^^ In contrast , both cell types produced IL 6 in response to TNF alpha , IFN gamma or IL 4 , and IFN gamma and IL 4 exhibited synergy with TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The host cytokines include TNF alpha , IL 1 , IL 6 , IFN gamma , and D factor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , the influence of inflammatory cytokines [ interleukin 1 beta ( IL 1 beta ) , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) ] on CA 125 release in normal and malignant cells was also studied . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , reverse transcription PCR analysis revealed that L selectin cells expressed interleukin 2 , IFN gamma and tumor necrosis factor alpha mRNA upon in vitro stimulation with specific tumor cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In a human macrophage cell line , TNF alpha exerted a stimulatory effect on viral replication and programmed cell death induced by HIV 1 which was potentiated by the simultaneous incubation with IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using three colour flow cytometry , we have measured intracellular IL 2 , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) induced in human CD4+ and CD8+ T cells from normal donors and patients with common variable immunodeficiency ( CVID ) . ^^^ Kinetic studies of activation with PMA and ionomycin show that a higher proportion of normal CD4+ cells can make IL 2 than the other two cytokines , and that there are more TNF alpha positive CD4+ cells than cells with IFN gamma . ^^^ Finally , using optimal conditions , we compared cytokine production in cells from patients with the disease CVID and showed normal cellular levels of ability to produce IL 2 and TNF alpha but significantly raised levels of production of IFN gamma in both CD4+ and CD8+ lymphocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our results showed IL 1 beta , IL 4 and IL 6 mRNA expression in three out of four tissue samples , whereas IL 1 alpha , IL 2 , IL 8 , IL 10 , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) were expressed in all tissue samples studied . ^^^ However , IL 2 , IL 4 , IL 6 , IL 8 , IFN gamma and TNF alpha mRNA were detected in both CD4+ and CD8+ subsets . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study has examined the levels of CRP , neopterin , soluble IL 2R , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) in cord blood samples from both premature and term neonates . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , anti TNF alpha antibody , which completely neutralized biological activity of the secreted TNF alpha , neither inhibited nitrite production nor restored CT replication in the LPS and / or IFN gamma treated RAW264 . 7 cells . ^^^ In mouse peritoneal macrophages treated with IFN gamma , both L NMMA and anti TNF alpha antibody inhibited nitrite production and restored CT replication . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore both childrens ' T cells were unable to produce the cytokines IL 2 , interferon gamma ( IFN gamma ) , IL 4 and tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Large amounts of IL 2 , IFN gamma , and TNF were produced in addition to an increase in IL 2 mRNA as a result of CD 80 costimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Elevated concentrations of IL 2 , IFN gamma , TNF beta , and IL 5 have been recently demonstrated in BP blister fluid . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We studied the expression of IL 1 ( Interleukin 1 ) , IL 2 , IL 4 , IL 10 , GM CSF ( granulocyte / macrophage colony stimulating factor ) , TNF alpha ( tumor necrosis factor alpha ) and IFN gamma ( Interferon gamma ) in tumor specimens and adjacent mucosa from 12 patients with squamous cell carcinoma of the head and neck ( SCCHN ) by reverse transcribed polymerase chain reaction ( RT PCR ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) further increased the CD 95 expression induced by KL+GM CSF . ^^^ The functional status of CD 95 and the effects of TNF alpha and IFN gamma , cytokines known to induce CD 95 mediated apoptosis , were analyzed by incubation of PHPs in the presence of anti CD 95 monoclonal antibodies ( MoAbs ) . ^^^ A decrease of CFU C numbers was observed in the presence of anti CD 95 MoAbs and TNF alpha and / or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Proliferation of OSE cells remained unaffected by interferon ( IFN ) alpha or IFN gamma , whereas interleukin 1 ( IL 1 ) and tumor necrosis factor ( TNF ) increased cell growth . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Ridostin activated IFN alpha and suppressed TNF alpha and IFN gamma production by these cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
ICAM 1 expression was upregulated by TNF alpha , Il 1 beta and IFN gamma . ^^^ VCAM 1 expression was increased by TNF alpha and weakly by Il 1 beta , IFN gamma had no effect on VCAM 1 expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : We assessed TIL expression of interleukin 2 ( IL 2 ) , IL 4 , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , IL 10 , and transforming growth factor beta ( TGF beta ) , and tumor cell expression of IL 10 and TGF beta in situ in 49 primary colon carcinomas and 20 metastases using immunohistochemistry . ^^^ RESULTS : The percentage of primary colon carcinoma samples in which > 20 % of TIL expressed each cytokine was as follows : IL 4 : 47 % ; TNF alpha : 22 % ; TGF beta : 10 % ; IFN gamma : 6 % ; IL 2 : 2 % ; IL 10 : 0 % ; and GM CSF : 0 % . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Islet grafts from CFA injected mice contained fewer CD4+ and CD8+ cells and more B cells ; also fewer interferon gamma ( IFN gamma ) , interleukin 2 ( IL 2 ) , and tumor necrosis factor alpha ( TNF alpha ) positive cells and more IL 4 and IL 10 positive cells . ^^^ These findings suggest that destruction of beta cells in syngeneic islets transplanted into NOD mice is promoted by cells producing Th 1 type cytokines ( IFN gamma , IL 2 , and TNF alpha ) and prevented by cells producing TH 2 type cytokines ( IL 4 and IL 10 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Staining for lymphocytes , macrophages , and tumor necrosis factor ( TNF ) alpha did not show differences between the groups , but IFN gamma was markedly less expressed in IL 4 and IL 10 treated grafts . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha gene expression in the liver of the IFN gamma transgenic mouse with chronic active hepatitis . ^^^ IFN gamma mRNA was found to be expressed in the transgenic mouse liver along with TNF alpha mRNA . ^^^ Since TNF alpha is a terminal mediator of liver injury in several animal liver injury models , its high expression , presumably a result of the IFN gamma which is transcribed from the introduced gene , might be involved in the development of chronic active hepatitis in this transgenic mouse . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and interleukin 1 ( IL 1 ) stimulate adhesion receptor expression on lymphoid and nonlymphoid tissues . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Bronchoalveolar and peripheral blood mononuclear cells were cultured in serum free medium , without stimulant , for 24 h , and the supernatants analysed for concentrations of interleukin ( IL ) 1 beta ( IL 1 beta ) , IL 2 , IL 6 , tumour necrosis factor alpha ( TNF alpha ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , interferon gamma ( IFN gamma ) and neopterin . ^^^ Bronchoalveolar lavage cells ( BALC ) of sarcoid patients released significantly higher amounts of TNF alpha , IL 6 , IFN gamma and neopterin in comparison to normal controls . ^^^ In the sarcoid group , the levels of IL 1 beta , IL 6 , TNF alpha and GM CSF showed highly significant correlations between each other , but not with IL 2 , IFN gamma or neopterin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
During the first 129 days of infection , high levels of cytokines TNF alpha , IL alpha , IFN gamma , IL 6 , and IL 10 and specific IgG 1 and IgG 3 isotypes were detected , as compared to uninfected controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we evaluated the ultrastructural alterations of murine microglia that were associated with activation by interferon gamma ( IFN gamma ) plus tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , other cytokines , such as IFN gamma , TNF beta and TNF alpha , can rather compromise pregnancy . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Specific antibodies including anti IFN gamma , anti TNF and anti IL 6 antibodies , as well as the cyclooxygenase inhibitor indomethacin , have been used to reverse cancer cachexia . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Role of IFN gamma on TNF alpha , IL 1 beta and IL 6 release during HHV 6 infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The inhibition was specific : when tested at 10 microM , pentamidine had no significant inhibitory effect on the PHA induced generation of the non chemotactic cytokines tumor necrosis factor alpha ( TNF alpha ) , IL 1 beta , IL 2 , IL 4 , IL 5 , IL 10 and interferon gamma ( IFN gamma ) , except for a partial inhibition on IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
HUVEC were incubated for 24 h in media containing interleukin 1 beta ( IL 1 beta ) , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , or interleukin 6 ( IL 6 ) , and thrombin stimulated increases in [ Ca2+ ] 1 and PGI 2 production were then examined . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using human thyrocytes in primary cultures , we show that expression of MCP 1 mRNA and protein is remarkably stimulated by both interleukin 1 ( IL 1 ) and tumor necrosis factor alpha ( TNF alpha ) , and also that interferon gamma ( IFN gamma ) by itself is a weak stimulant but has a synergistic activity with either IL 1 or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These effects were partially mediated by either tumor necrosis factor alpha or interleukin 1 , because AmB enhanced IFN gamma induced production of these cytokines by macrophages and their specific antibodies partially inhibited the AmB induced enhancement of NO generation when they were used separately . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To determine whether an improvement in skin lesions as a result of PUVA therapy may be correlated with changes in cytokine patterns , RT PCR amplification was used to compare the levels of IL 2 , IL 6 , IL 8 , IL 10 , TNF alpha and IFN gamma cytokine mRNA expression in serial biopsies from three chronic plaque psoriatic patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokines examined and the mRNA positivity were : interleukin ( IL ) 1 beta ( 21 % ) , IL 2 ( 0 % ) , IL 3 ( 7 % ) , IL 4 ( 41 % ) , IL 5 ( 17 % ) , IL 6 ( 53 % ) , IL 8 ( 98 % ) , interferon gamma ( IFN gamma ) ( 69 % ) , and tumor necrosis factor alpha ( TNF alpha ) ( 24 % ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increases in TNF alpha , IL 6 , IL 1 beta , and IFN gamma mRNAs were found in the livers of patients with extrahepatic diseases . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast cytokines such as TNF alpha , IFN gamma , IL 10 and IL 15 had no detectable effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The synthesis of IL 2 , IFN gamma and TNF alpha was strongly increased in A 60 primed mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Of the cytokines tested on AM , IFN gamma increased the release of TNF sR 75 3 fold , but that of TNF sR 55 only between 1 . 5 and 2 fold . ^^^ Addition of colchicine increased the release of TNF sR 75 induced by LPS and IFN gamma , but not by PMA . ^^^ Finally , IFN gamma was among the most efficacious cytokines to induce the release of both receptors , with TNF sR 75 being more liable to shedding . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We conclude that the influence of tolerance on the anti tumor activity of TNF as a single agent depends on the tumor type ; in combination therapy with IFN gamma , however , tolerance allowed us to dissociate lethal toxicity from anti tumor activity , irrespective of the tumor type tested . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The action of interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) lifted the block of MHC class 1 complex formation by stimulating synthesis , assembly and stability of MHC class 1 molecules . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Prior to the in vivo experiments the antiproliferative effects of human IL 2 , and of murine IFN gamma and TNF alpha which are cytokines induced by IL 2 were tested in a colony forming assay . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Previously it was established that L 1210 mouse leukemia and a variety of other tumor cell types produced a soluble factor ( s ) , designated tumor derived recognition factor ( TDRF ) , which synergized with interferon gamma ( IFN gamma ) and interleukin 2 ( IL 2 ) or lipopolysaccharide ( LPS ) to promote increased tumor necrosis factor alpha ( TNF alpha ) and nitric oxide synthase ( NOS ) mRNA synthesis by murine macrophages ( M phi ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both constitutive and inducible forms of NO synthase are expressed by bone derived cells , and cytokines such as interleukin 1 ( IL 1 ) , tumor necrosis factor ( TNF ) , and interferon gamma ( IFN gamma ) , are potent stimulators of NO production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
They contained IL 1 , IL 5 , IL 6 , IL 7 , IL 12 , GM CSF , M CSF , transforming growth factor beta ( TGF beta ) , TNF alpha , and TNF beta mRNAs but lacked IL 2 , IL 3 , IL 4 , IL 10 , and IFN gamma mRNAs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the production of interferon gamma ( IFN gamma ) , interleukin 4 ( IL 4 ) , IL 1 alpha , and tumor necrosis factor alpha ( TNF alpha ) by peripheral blood mononuclear cells ( PBMCs ) from human T lymphotropic virus type 1 ( HTLV 1 ) carriers during short term in vitro culture , in comparison with that by those from HTLV 1 seronegative controls . ^^^ CD4+ cells were responsible for the enhanced IFN gamma production , while monocytes were responsible for the increased IL 1 alpha and TNF alpha production . ^^^ The present study showed that the PBMCs from HTLV 1 carriers consistently produced large amounts of some cytokines ( IFN gamma , IL 1 alpha , TNF alpha ) but not of other cytokines ( IL 4 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine production in tonsil tissue in 4 patients with fulminant IM and respiratory tract obstruction showed a concomitant expression of IL 2 , IFN gamma , IL 6 , TNF beta , transforming growth factor ( TGF ) beta 1 3 , granulocyte colony stimulating factor , granulocyte macrophage colony stimulating factor , IL 4 IL 1alpha , IL beta and TNF alpha . ^^^ The number of IL 2 , IFN gamma , IL 6 and TNF beta producing cells was significantly higher compared to tonsil tissue obtained from children with tonsillar hypertrophy . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Production and specificity of antibodies , cytotoxic responses of macrophages and NK cells , spontaneous production ex vivo of cytokines IL 1 alpha , IL 2 , IL 4 , IL 6 , IL 10 , IFN gamma , and TNF alpha in spleen cell cultures in C3H / HeJ ( Lps ( d ) ) mice in comparison with C3H / HeN ( Lps ( n ) ) mice were tested . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of mRNA for TNF alpha , IL 12 , IFN gamma , and IL 10 was demonstrated within 48 h of inoculation , the kinetics being similar irrespective of bacterial strain used . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inhibition of the multiplication of Listeria monocytogenes in a murine hepatocyte cell line ( ATCC TIB 73 ) by IFN gamma and TNF alpha . ^^^ The effects of IFN gamma and TNF alpha on intracellular multiplication of Listeria monocytogenes in a murine enbryonic hepatocyte cell line ( ATCC TIB 73 ) were investigated . ^^^ Neither IFN gamma nor TNF alpha alone ( 10 , 25 , or 100 ng / ml ) significantly inhibited intracellular multiplication of L . monocytogenes . ^^^ In contrast , addition of both IFN gamma and TNF alpha ( 10 , 25 , or 100 ng / ml ) significantly inhibited intracellular listerial multiplication . ^^^ The anti listerial effects of IFN gamma and TNF alpha were not blocked by adding aminoguanidine or catalase . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum levels of tumor necrosis factor alpha ( TNF alpha and nitrite / nitrate in IFN gamma treated mice were similar to those of controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pleural fluid ( Pf ) from patients with tuberculosis , rheumatoid arthritis ( RA ) and other diseases were compared with respect to interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) . ^^^ Immunoassays were used to determine Pf IFN gamma and Pf TNF alpha in 102 patients , including 11 with RA , 31 with verified tuberculosis , 23 with suspected tuberculosis , 11 with pneumonia , 14 with lung cancer and 12 with congestive heart failure . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To understand how KGF might be regulated during wound healing , we examined the ability of the pro inflammatory cytokines interleukin 1 alpha ( IL 1 alpha ) , interleukin 1 beta ( IL 1 beta ) interleukin 6 ( IL 6 ) , tumor necrosis factor alpha ( TNF alpha ) transforming growth factor beta 1 ( TGF beta 1 ) and interferon gamma ( IFN gamma ) to modulate KGF gene expression in cultured human fibroblasts , using northern blot analysis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We analysed the cytoskeletal proteins and extracellular matrix components of in vitro cultured BMSC both in resting state and after activation with IFN gamma and TNF alpha , using an immunoperoxidase procedure . ^^^ This pattern of expression was not affected by addition of TNF alpha and IFN gamma , but differs from human tonsil stromal cells for laminin expression and alpha actin localization . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
On the other hand , modulation of VnR expression by interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) was observed on different non melanoma cell lines . ^^^ We tested IFN gamma , TNF alpha and interleukin 2 ( IL 2 ) , which are presumably released by infiltrating leukocytes in the melanoma lesional environment , on three melanoma cell lines . ^^^ By contrast , the expression of the intercellular adhesion molecule 1 ( ICAM 1 ) , tested as control , on five melanoma cell lines , was greatly enhanced by IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We used semiquantitative RT PCR to monitor the expression of mRNA encoding cytokines ( IL 1 beta , IL 6 , TNF alpha , and IL 10 ) and IFN gamma in fresh isolated peripheral blood mononuclear cells ( PBMCs ) , lymph node mononuclear cells ( LNMCs ) , and mononuclear cells obtained after bronchoalveolar lavages ( BALMCs ) , of four cynomolgus macaques inoculated intravenously with a pathogenic isolate of simian immunodeficiency virus ( SIVmac 251 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Spleen cells from sensitized IFN gammaR / mice proliferated extensively when stimulated with MOG peptide in culture and produced high levels of IFN gamma and TNF but no detectable IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 2 , TNF alpha , IFN gamma , IL 10 , and RANTES mRNAs were readily detected in TGs taken from mice 7 days postinoculation ( PI ) . ^^^ Between 5 and 45 days PI , IL 10 , IFN gamma , TNF alpha , and RANTES mRNAs were detected in nearly 100 % of latently infected TGs ( latent infection was confirmed by reverse transcription PCR detection of HSV 1 latency associated transcripts ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The inducing signals for astrocyte NOS 2 mRNA expression were in the order IL 1beta + IFN gamma > IL 1beta + TNF alpha > IL 1beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To elucidate the molecular mechanisms regulating the anti inflammatory activities of recombinant human ( rh ) IL 11 , the ability of rhIL 11 to reduce serum levels of inflammatory mediators such as TNF alpha , IL 1beta , IL 12 , and IFN gamma in LPS treated mice and to down regulate macrophage function in culture was investigated . ^^^ In a mouse model of endotoxemia , pretreatment with rhIL 11 blocked LPS induced elevation of TNF alpha , IL 1beta , and IFN gamma serum levels , but had no effect on IL 12 p 40 , IL 6 , or IL 10 serum levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
While beta casein had no significant effects on IFN gamma production by ovine blood lymphocytes , and TNF alpha production and MCH Class 2 antigen expression by ovine bronchoalveolar macrophages , it enhanced IL 1 beta production by the macrophages , beta casein also had no influence on bovine NK cell activity against a virally infected cell line . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MS is associated with the parallel up regulation of proinflammatory [ interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , lymphotoxin alpha , and interleukin ( IL ) 12 ] and immune response down regulating [ transforming growth factor beta ( TGF beta ) , IL 10 ] cytokines systemically . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Because an important characteristic of DTH responses is the liberation of potentially injurious cytokines into adjacent tissues , we also examined the effects of mouse recombinant tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 alpha ( IL 1 alpha ) , and interferon gamma ( IFN gamma ) on cultured OLGs and immortalized OLGs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effect of LPS , IFN gamma and IL 4 on CD 14 expression differes from their effect on expression of the immune accessory molecules B 7 1 and HLA DR , and on production of tumor necrosis factor alpha ( TNF alpha ) , whose secretory pathway is similar to that of CD 14 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha was shown to have direct cytotoxic effect on glial cells , which was greatly enhanced by small amounts of interferon gamma ( IFN gamma ) . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation of cells from MBP 73 84 : 1028 coimmunized protected rats proliferate and secrete interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) in vitro in response to MBP 73 84 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) partially inhibited TNF alpha induced IL 8 secretion , whereas no influence on IL 8 mRNA levels was detected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma produces morphologic changes similar to those induced by TNF but does not cause surface protein redistribution . ^^^ Cells treated with TNF plus IFN gamma become even more elongated and display TNF like redistributions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
HOF contained IL 2 , IL 6 , IL 8 , TNF alpha , MIP 1 alpha , IFN gamma , and high levels of IL 1 beta , IL 10 , IL 1RA , and sIL 2R . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Exposure to cytokines ( TNF , IFN gamma , IL 1 alpha , and IL 6 ) and lipopolysaccharide resulted in an increase in expression of ICAM 1 , VCAM , E selectin , and CD 36 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 alpha ( IL 1 alpha ) , and interleukin 6 ( IL 6 ) on the growth of the Toxoplasma gondii RH strain were studied in vitro using a human astrocytoma derived cell line . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) inhibit the growth of the human papillary thyroid carcinoma ( PTC ) cell line , NP . ^^^ In contrast , IFN gamma increased shedding of both p 55 and p 75 TNF alpha receptors in NP and R 60 cell lines with equal potency . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , enhancement of TNF alpha production by IFN gamma was also suppressed by rMNSF beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human umbilical vein endothelial cells were incubated in vitro for 1 h . at 39 , 42 , 43 and 44 degrees C with and without addition of tumour necrosis factor ( TNF ) or interferon gamma ( IFN gamma ) and the expression of endothelial cell leukocyte adhesion molecule 1 ( ELAM 1 ) , intercellular adhesion molecule 1 ( ICAM 1 ) and major histocompatibility complex ( MHC ) class 2 molecule was measured . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Complement synthesis in cultured chondrocytes was modulated by the cytokines interleukin 1 beta ( IL 1 beta ) , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) , showing that cytokines can probably regulate complement synthesis in intact cartilage . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Growth arrested cell cultures were stimulated with the human recombinant cytokines interleukin 1 alpha ( IL 1 alpha ) , tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , interleukin 13 ( IL 13 ) , interleukin 10 ( IL 10 ) or vehicle added alone or in combination . ^^^ HT 29 cells produced IL 8 following stimulation with IL 1 alpha or TNF alpha in a time and a concentration dependent manner , while IFN gamma , IL 10 and IL 13 did not induce IL 8 production by HT 29 cells . 5 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha also enhanced interferon gamma ( IFN gamma ) induced complement C 2 production by ME F 1 and ME F 3 cells but did not affect production by differentiated ME F 1 and ME F 3 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum concentrations of interleukin 1 beta ( IL 1 beta ) , interleukin 6 ( IL 6 ) , interleukin 8 , ( IL 8 ) , tumour necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) were measured before and after surgery using enzyme linked immunosorbent assays . ^^^ The serum concentrations of IL 1 beta , IL 8 , TNF alpha , and IFN gamma did not change significantly following neurosurgical operations . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Contrasting response of lung parenchymal cells to instilled TNF alpha and IFN gamma : the inducibility of specific cell ICAM 1 in vivo . ^^^ In this report , we examined the effects of tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) on specific lung cell expression of ICAM 1 in vivo and the accompanying morphological changes . ^^^ Balb c mice were treated with phosphate buffered saline ( PBS ) alone or with PBS containing 5 micrograms TNF alpha or IFN gamma through intranasal instillation . ^^^ Topical treatment of the lung with either TNF alpha or IFN gamma induced a 50 60 % increase in total lung and alveolar ICAM 1 . ^^^ These results showed that TNF alpha and IFN gamma induce ICAM 1 expression and infiltration of neutrophils in the lung . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although exposure to LPS , interleukin 11 ( IL 11 ) , tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) and IL 1 , over the physiological range of concentrations , resulted in a decrease in the heparin releasable LPL activity , LPL mRNA levels and LPL protein content of the cells , stimulation with IL 6 and leukaemia inhibitory factor ( LIF ) had no effect . ^^^ The maximum suppression of LPL activity and mRNA levels in the cells by IFN gamma ( 60 % ) was lower than that produced by LPS , IL 11 , TNF alpha and IL 1 ( 78 97 % ) . ^^^ Each cytokine displayed a characteristic dose dependent pattern for the suppression of LPL activity and mRNA levels with IL 11 / TNF alpha being more potent than IFN gamma / IL 1 . ^^^ More than 80 % of the decrease in the LPL activity , at all doses of IL 11 , TNF alpha , IFN gamma and IL 1 , was due to a corresponding reduction in the mRNA levels . ^^^ The time course of responses to LPS , IL 11 , TNF alpha , IFN gamma and IL 1 were similar , with the time required to achieve half maximal suppression of LPL activity being between 7 and 9 . 5 h in each case . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This salutary CTLA4Ig effect correlated with markedly diminished TSST 1 induced serum levels of TNF alpha and IFN gamma , but TSST 1 triggered IL 2 release was not affected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interestingly , this factor is not likely to be IL 2 , IL 4 , IL 6 , IL 10 , IL 15 , TNF alpha , or IFN gamma as Abs against these cytokines were not able to inhibit the contact independent B cell help by CHCD 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Hyperoxia activates NF kappaB and increases TNF alpha and IFN gamma gene expression in mouse pulmonary lymphocytes . ^^^ No increases in cytokine ( IL 1beta , IL 6 , IL 10 , TGF beta , TNF alpha , IFN gamma ) expression nor in NF kappaB activation were found after 12 h of hyperoxia . ^^^ By 48 h of hyperoxia , amounts of IFN gamma and TNF alpha protein as well as mRNA were increased in the lungs , and NF kappaB continued to show activation , even though no histologic abnormalities were present . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To evaluate the potential of macrophages to participate in such matrix destruction , we studied the effects of three cytokines present in inflammatory tissue sites , TNF alpha , IL 1beta , and IFN gamma , on the production of three matrix degrading metalloproteinases , interstitial collagenase , stromelysin , and 92 kDa gelatinase , as well as their natural inhibitor , TIMP 1 ( tissue inhibitor of metalloproteinases number 1 ) , by human monocyte derived macrophages differentiated in vitro . ^^^ IFN gamma suppressed the production of the 92 kDa gelatinase induced by TNF alpha and IL 1beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In an attempt to clear critical cytokines in the development and maintenance of sarcoid granuloma , we have measured the level of seven cytokine mRNA ( TNF alpha , IL 6 , IL 8 , TGF beta , PDGF B , IFN gamma , and GM CSF ) in cells obtained by BAL from sarcoidosis patients and normal subjects . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunofluorescence studies on TNF alpha and / or IFN gamma treated HRPE cells demonstrated cellular expression of ICAM 1 which was predominantly localized to intercellular junctions . ^^^ Moreover , treatment of HRPE for 24 h with tumour necrosis factor alpha ( TNF alpha ) ( 10 ng / ml ) , interferon gamma ( IFN gamma ) ( 500 u / ml ) , interleukin 1 alpha ( IL 1 alpha ) ( 10 ng / ml ) and IL 1 beta ( 10 ng / ml ) results in the secretion of ICAM 1 , ranging from 9 to 13 ng per 10 ( 6 ) cells . ^^^ IFN gamma acts synergistically with ( TNF alpha ) and IL 1 in the secretion of ICAM 1 by HRPE . ^^^ Northern blot analysis reveals an increased expression of ICAM 1 mRNA in HRPE stimulated with IFN gamma , TNF alpha or IL 1 for 24 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Citrulline ( 0 . 1 1 . 0 mM ) or arginine ( 0 . 1 1 . 0 mM ) led to a similar dose dependent nitric oxide ( NO ) production by rat islets exposed to interleukin 1 beta ( IL 1 beta ) or human islets exposed to IL 1 beta + tumour necrosis factor alpha ( TNF alpha ) + interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The production of cytokines was determined in cultures after 24 h [ IL 1 alpha , IL 4 , IL 5 , IL 6 , tumour necrosis factor alpha ( TNF alpha ) , and TNF beta ] or 72 h ( IL 2 , IFN gamma ) . ^^^ The combination ION + PMA induced the highest levels of IL 2 , IL 4 , IL 5 , IFN gamma and TNF alpha , whereas maximal production of IL 6 and TNF beta were induced by PHA and PHA + PMA , respectively . ^^^ The AD group demonstrated a significantly lower production of TNF alpha and IFN gamma compared with the two other groups , and IL 4 and IL 5 production increased in the IA group . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This synthesis was not associated with the release of IL 4 , IL 12 , interferon gamma ( IFN gamma ) , the three colony stimulating factors ( CSFs ) or the soluble TNF receptors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IgE , TNF alpha , IL 1 beta , IL 4 and IFN gamma gene polymorphisms in sheep selected for resistance to fleece rot and flystrike . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The in vitro production of TNF alpha ( tumor necrosis factor alpha ) , IL 2 ( interleukin 2 ) and IFN gamma ( interferon gamma ) by spleen cells , and the levels of serum sIL 2R ( soluble IL 2 receptor ) were also measured . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Further , we have recently shown that TNF alpha and interferon gamma ( IFN gamma ) act synergistically to block TSH induced increases in type 1 5 ' D 1 activity and 5 ' D 1 gene expression in FRTL 5 rat thyroid cells . ^^^ Thus , we tested the hypothesis that the activation of one or both pathways is involved in synergistic effect of TNF alpha and IFN gamma on thyroid function . ^^^ The activation of additional signal transduction pathways , however , by TNF alpha is required for the synergistic inhibition of thyroid function by TNF alpha and IFN gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
High serum levels of IL 2 , TNF alpha , IFN gamma and strong induction of CTLs ( cytotoxic T lymphocytes ) were noted after priming with the fusion protein . ^^^ T cells responded well to 3 daily injections of C215Fab SEA and then gradually entered a hyporesponsive state , characterized by a reduced ability to produce IL 2 , TNF alpha and IFN gamma and failure to mediate cytotoxicity in vitro . ^^^ Intermittent treatment was characterized by increased levels of IL 10 , concomitant with accentuated loss of IL 2 , TNF alpha and IFN gamma production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The CD4+ T cell clone designated YS 1093 secreted interleukin ( IL ) 4 , IL 5 , and IL 6 , but not IFN gamma , tumor necrosis factor alpha , or IL 2 , thus indicating that the clone belongs to the Th 2 type . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Maternal cells in breast milk expressed mRNA for MCP 1 ( 20 / 20 ) , IL 8 ( 14 / 20 ) , TGF beta 1 ( 14 / 16 ) , TGF beta 2 ( 4 / 6 ) , M CSF ( 9 / 12 ) , IL 6 ( 6 / 12 ) and IL 1 beta ( 7 / 12 ) , and may be a source of these cytokines . mRNA for IL 2 , IL 10 , IFN gamma , TNF alpha was not detected and only weak expression was found for RANTES ( 1 / 18 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Conversely , pretreatment ( 2 h ) of mice with an anti IL 10 monoclonal antibody ( 2 mg / mouse ) was associated with increased lung levels of TNF and IFN gamma , reduced bacterial counts in lungs and plasma 40 h after the inoculation , and prolonged survival . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , co stimulation of the respective T cell populations with anti CD 28 antibodies resulted in the generation of IFN gamma , IL 4 and TNF alpha , but not IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In studies with triggered human blood mononuclear cells in culture , glucocorticoids strongly diminish the production of the ' initial phase ' cytokines IL 1 beta and TNF alpha and the ' immunomodulatory ' cytokines IL 2 , IL 3 , IL 4 , IL 5 , IL 10 , IL 12 and IFN gamma , as well as of IL 6 , IL 8 and the growth factor GM CSF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Culture of both control and transplant derived PBMC in PHA + IL 12 ( 20 U / ml ) or IL 2 ( 100 U / ml ) + IL 12 ( 20 U / ml ) combinations yielded comparable production of IFN gamma , one of the major biological effects of IL 12 in vivo , as well as equal production of TNF alpha , a costimulatory factor of IL 12 mediated induction of IFN gamma by NK cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma did not increase the low level of endogenous TNF alpha , which is not secreted by embryonic cells , but stimulated the expression of TNF alpha R 1 , although to a relatively low extent . ^^^ IFN gamma facilitated TNF alpha response through an increase in TNF alpha R 1 expression , but probably also through interaction with different intracellular signal transduction pathways . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the first experiment , lymph nodes ( LN ) obtained from animals sacrificed at early time points following intravenous ( i . v . ) inoculation with a high viral dose , showed that the production of cytokine mRNA in LN ( IFN gamma , IL 1 beta , IL 2 , IL 4 , IL12p40 , IL 6 , IL 10 , TNF alpha and TNF beta ) was correlated with viral replication and persisted during the two months post inoculation ( p . i . ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the absence of the HSR , combinations of interleukin 1beta ( IL 1beta ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) stimulated high levels of NOS 2 mRNA and nitric oxide ( NO ) synthesis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum concentrations of tumour necrosis factor alpha ( TNF alpha ) , interleukin 1 receptor antagonist ( IL 1ra ) , interferon gamma ( IFN gamma ) , interleukin 6 ( IL 6 ) and interleukin 10 ( IL 10 ) were studied in 31 patients with haematological malignancies during febrile neutropenia . ^^^ Increased levels of all cytokines were detected after start of fever with peak values in gram negative ( Gr ) bacteraemias after 2 h ( TNF alpha , IL 1ra and IFN gamma ) , 4 h ( IL 6 ) and 6 h ( IL 10 ) , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation with tumor necrosis factor a ( TNF alpha ) and interferon gamma ( IFN gamma ) resulted , in addition to interleukin ( IL ) 4 , in selective upregulation of ICAM 1 expression . ^^^ Costimulation of either IL 4 or IFN gamma with TNF alpha further increased the ICAM 1 expression as compared to the stimuli alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Under natural culture conditions , microglia expressed the immunosuppressive transforming growth factor beta 1 ( TGF beta 1 ) and interleukin ( IL ) 10 to a greater degree than the pro inflammatory cytokines IL 1 beta , IL 6 , IL 12 , interferon gamma ( IFN gamma ) and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
C57BL / 6 IFN gamma knockout mice ( IFN gamma 0 / 0 ) produced as much circulating IL 12 p 40 and IL 12 p 70 as did IFN gamma + / + mice following injection with S . enteritidis LPS , despite sustaining 11 fold reductions in circulating TNF alpha . ^^^ Pretreatment of IFN gamma 0 / 0 mice with recombinant mouse IFN gamma ( rIFN gamma ) enhanced circulating TNF alpha by as much as sixfold , but serum IL 12 p 40 and IL 12 p 70 responses increased by only twofold or less . ^^^ These data suggest that the aggregate IL 12 p 40 and p 70 response to endotoxemia in vivo is IFN gamma independent and distinct from IFN gamma dependent serum TNF alpha and splenic IL 12 responses . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , treatment with three inflammatory cytokines , IL 1 beta , TNF alpha , and IFN gamma , produced clear increases in the levels of alpha 1 and alpha 5 present on the cell surface . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Changes in cytokine production of IL 1 beta , IL 2 , IL 6 , IL 10 , IFN gamma , and TNF alpha were studied using the ELISA method . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To investigate the molecular mechanism by which cytokines may sensitise multidrug resistant colon carcinoma cells , HCT 15 and HCT 116 , to treatment with MDR related drugs , we evaluated the effects of the human cytokines tumour necrosis factor alpha ( TNF alpha ) , interleukin 2 ( IL 2 ) and interferon gamma ( IFN gamma ) on mdr 1 gene expression at the mRNA level by reverse transcription polymerase chain reaction ( RT PCR ) and at the protein level with monoclonal antibodies by immuno flow cytometry . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , the mice were protected against LPS and TNF alpha induced lethality with a single dose of an anti TNF alpha or anti IFN gamma mAb given at the time of priming . ^^^ Our results suggest that TNF alpha and IFN gamma are produced early after priming with C . parvum and are at least partly responsible for the enhanced sensitivity of the mice to LPS and TNF alpha . ^^^ IL 10 affords protection to the mice because of its ability to block the C . parvum induced TNF alpha and IFN gamma responses . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Anti IFN gamma antibody ( Ab ) did not block the IGIF induced cytotoxicity of Th 1 cells , nor did IFN alpha , IFN gamma , or TNF alpha augment the cytotoxic activity of Th 1 , thus indicating that this enhanced cytotoxicity of Th 1 cells was mediated by IGIF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Since EGF receptors in other tissues can be up regulated by other growth factors and by cytokines , we have in this paper investigated whether such a beta cell responsiveness to TGF alpha , or EGF , can be conferred by co culture with interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) or transforming growth factor beta ( TGF beta ) in various combinations . ^^^ TNF alpha ( 1000 U / ml ) , on the other hand , affected none of these parameters either alone or in any combination with TGF alpha or IFN gamma . ^^^ However , when TNF alpha or IFN gamma , either alone or in combination , were combined with the cytokine interleukin 1 beta , this resulted in islet disintegration , whereas the latter cytokine alone did not exert any gross necrotic changes evident by light microscopy . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
An interleukin 12 ( IL 12 ) driven induction of interferon gamma ( IFN gamma ) , probably in synergism with endogenous TNF , was found to mediate infection induced sensitization . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Infective , stationary growth phase metacyclic promastigotes of Leishmania major were introduced to murine peritoneal macrophages and incubated with recombinant murine tumour necrosis factor ( TNF ) or with interferon gamma ( IFN gamma ) in the presence of LPS . ^^^ The results showed that both ( TNF ) and ( IFN gamma ) had a significant leishmanicidal effect , yet it was more pronounced with IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Semi quantitative competitive RT PCR of liver homogenates obtained 3 weeks after infection revealed that depletion of CD4+ T cells was accompanied by a 25 fold reduced expression of IFN gamma mRNA and a 5 fold reduced expression of tumor necrosis factor ( TNF ) alpha mRNA when compared to control infected mice . ^^^ The delayed kinetics of granuloma initiation in infected SCID mice was paralleled by a lower mRNA expression for IFN gamma and TNF alpha compared to that observed in infected BALB / c mice . mAb mediated neutralization of IFN gamma in BALB / c mice significantly reduced inflammatory infiltrations and granuloma formation . ^^^ These data support the conclusion that CD4+ T cells accelerate granuloma formation by enhancing the production of TNF alpha and IFN gamma at the site of infection . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of SC is regulated by various inflammatory cytokines such as , IL 1 , IL 4 , IL 6 , IL 8 , IFN gamma and TNF alpha , suggesting SC upregulation in vivo in inflammatory conditions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Both non transformed and SV 40 transformed human fetal RPE cells express IL 15 , a T cell growth factor which has similar biological activities to IL 2 , and the expression of IL 15 is enhanced by interferon gamma ( IFN gamma ) or tumor necrosis factor alpha ( TNF alpha ) stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We showed that IFN gamma and tumor necrosis factor ( TNF ) alpha cooperated to induce RANTES production by monocytes . ^^^ Both IL 10 and corticosteroids antagonized the stimulating effect of IFN gamma and TNF alpha on RANTES production . ^^^ In contrast , IL 4 had no effect on IFN gamma induced RANTES production and it potentiated the positive effect of TNF alpha on this production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It consists of 5 ' and 3 ' primer sequences in the order of beta 2 microglobulin ( beta 2 m ) , IL 1 , IL 4 , IL 6 , IL 8 , IL 2 , IL 10 , TNF alpha , TNF beta and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha , acting synergistically with cytokines such as IL 1 , GM CSF and IFN gamma , is the key mediator in the induction process of septic shock , as shown in several experimental models . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Peripheral blood mononuclear cells ( PBMC ) and tumor infiltrating lymphocytes ( TIL ) were tested for the natural killer ( NK ) cytotoxic activity , for the in vitro synthesis of interleukin 2 ( IL 2 ) , interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) and prostaglandin E 2 ( PGE 2 ) . ^^^ Instead , TIL produced significant larger amounts of spontaneous PGE 2 ( p < 0 . 001 ) and significant lower amounts of IFN gamma ( p < 0 . 001 ) and TNF alpha ( p < 0 . 001 ) than autologous PBMC , suggesting an involvement of PGE 2 in the impairment of the host immunoreactivity at the tumor site . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Antibody or peptide blockade against CD 36 , ICAM 1 , and thrombospondin or preincubation of target cells with TNF alpha and IFN gamma did not significantly alter the binding intensity of HB3EC 6 to HMEC 1 and HB3C32 6 to C 32 melanoma cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The anti inflammatory cytokines IL 4 and IL 10 potently suppressed IL 1 beta plus IFN gamma stimulated NO , while transforming growth factor beta preferentially inhibited IL 1 beta plus TNF alpha stimulated production of NO . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pretreatment of cultures with recombinant human tumor necrosis factor alpha , alpha interferon ( IFN alpha ) , IFN beta , or IFN gamma for 24 h prior to inoculation inhibited T . gondii replication in a dose dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Differential secretion of TNF alpha and IFN gamma by human peripheral blood derived NK subsets and association with functional maturation . ^^^ This study investigated the secretion of tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) by purified NK cells and NK subsets following activation by various stimuli . ^^^ K 562 target cells stimulated secretion of both TNF alpha and IFN gamma by both the binder and the killer subsets but not by the free subset . ^^^ IFN alpha activated the secretion of IFN gamma only , whereas IL 2 activated the secretion of both TNF alpha and IFN gamma by the binder and killer subsets and secretion was augmented by the addition of K 562 to the cultures . ^^^ Phorbol myristate acetate ( PMA ) and ionophore stimulated TNF alpha and IFN gamma secretion in both the binder and the killer subsets , though IFN gamma secretion was more pronounced in the binder subset . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of IL 8 , TNF alpha and IFN gamma m RNA in ulcerative colitis , particularly in patients with inactive phase . ^^^ METHODS : In 27 patients with UC , the mucosal cytokine expression of m RNA including IL 8 , TNF alpha , IFN gamma and IL 6 were examined using the reverse transcription coupled polymerase chain reaction ( RT PCR ) and oligonucleotide hybridization . ^^^ RESULTS : 1 ) The expression ratios of m RNA of IL 8 , IFN gamma and TNF alpha in cases with UC ( 92 . 6 % , 70 . 4 % and 77 . 8 % , respectively ) were significantly higher than in normal controls ( 17 . 6 % , 0 . 0 % and 5 . 9 % , respectively ) ( p < 0 . 01 ) . ^^^ Regarding IL 8 and IFN gamma , those in UC cases were also significantly higher than in cases with other types of colitis ( p < 0 . 05 ) . 2 ) No significant difference was noted between active and inactive cases with UC in terms of the expression ratios of m RNA of IL 8 , TNF alpha and IFN gamma . 3 ) Only the expression ratio of IL 6 m RNA correlated with the macroscopic score according to Matts . ^^^ CONCLUSION : The expression of IL 8 , TNF alpha and IFN gamma m RNA increased even in inactive UC , suggesting that these three cytokines play an important role in the pathogenesis of repeated inflammation of UC . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro TNF alpha , IL 2 and IFN gamma production as markers of relapses in multiple sclerosis . ^^^ In 22 patients with definite multiple sclerosis ( MS ) we determined with monthly intervals over a period of 24 months the in vitro tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and interleukin 2 ( IL 2 ) production and the serum neopterin levels . ^^^ There was a significant correlation with in vitro IFN gamma release , the absolute blood monocyte count and the serum neopterin levels , suggesting that monocytes stimulated by IFN gamma play an important role in the TNF alpha production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After 24 hours incubation with either tumor necrosis factors alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) or interleukin 1 beta ( IL 1 beta ) , astrocytic glutamate uptake was markedly attenuated in a dose dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There was varied expression of IL 1 beta , IL 4 , IL 6 , IL 10 and TNF alpha mRNA from the four HIV 1 infected LN and the patient undergoing seroconversion showed evidence for a mixed cytokine profile , which also included IL 2 and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The aim of the present work is to investigate the influence of IVIg on IFN gamma production occurring during MLR and its subsequent impact on T cell proliferation and tumor necrosis factor ( TNF ) alpha secretion . ^^^ We tested IVIg preparations for the presence of anti IFN gamma and anti TNF alpha antibodies . ^^^ High amounts of anti IFN gamma , but not anti TNF alpha antibodies , were found . ^^^ We conclude that the capacity of IVIg to inhibit proliferation and TNF alpha release during MLR is due to IFN gamma blockade by natural antibodies . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , immunocompetent BALB / c mice were found to generate an early IFN gamma response , coincident with a rise in IL 2 mRNA levels and elaboration of a tissue response involving TNF alpha production by infected Kupffer cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Trypanosoma cruzi : IL 10 , TNF , IFN gamma , and IL 12 regulate innate and acquired immunity to infection . ^^^ We investigated the roles of IL 10 , TNF , IFN gamma , and IL 12 in the control of parasitism by innate and specific immunity . ^^^ Treatment of infected RAG / KO , IL 10 / KO , and WT mice with anti IFN gamma , anti TNF , or anti IL 12 neutralizing mAbs increased parasitemia levels showing the importance of endogenous production of these cytokines in the control of parasitism by innate and specific immune responses . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the MLC , a feedback mechanism between TNF alpha and IFN gamma exists , since anti TNF gamma reduced the secretion of IFN gamma and anti IFN gamma inhibited the release of TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We observed the beryllium stimulated release of tumor necrosis factor alpha ( TNF alpha ) , interleukin 6 ( IL 6 ) , interleukin 2 ( IL 2 ) , and interferon gamma ( IFN gamma ) but not interleukin 4 ( IL 4 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Substantial numbers of Th 1 cells ( positive for IFN gamma , TNF alpha , IL 2 and / or IL 2 receptor ) were observed only after acceleration of diabetes development by a single injection of cyclophosphamide ( 250 mg / kg i . p . ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We examined the effects of histamine , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , indomethacin and dexamethasone on the adherence of eosinophils to airway epithelial cells . ^^^ Histamine significantly increased the adhesion of eosinophils to epithelial cells , while TNF alpha or IFN gamma did not significantly alter the adhesion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 4 neutralization or TNF alpha treatment ameliorate disease by an intracellular pathogen in IFN gamma receptor deficient mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 2 , IFN gamma , and TNF alpha mRNA levels were lower in CD 28 / mouse skin treated with phenol . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In particular , TNF and IFN gamma were implicated in the mechanism of death because their neutralization protected the mice from death , and recombinant IFN gamma and TNFalpha mimicked the lethal effect of SEB in influenza infected mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Northern blotting analysis showed that the expression of mRNA of C 3 and C 4 was enhanced by TNF alpha and IFN gamma , respectively , in AoSMC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the DLN of normal mice following the hapten application , sequential upregulation of cytokine mRNA including IL 1 alpha , IL 1 beta , IL 2 , IL 4 , IL 10 , IFN gamma and TNF alpha was found . ^^^ No change was seen for IL 1 alpha , IL 1 beta , IL 10 and TNF alpha and IL 2 , IL 4 and IFN gamma mRNA levels were below the level of detection in DLN from CD 4 mice following the hapten application . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
T cell secretion of interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) and TNF beta , or lymphotoxin contributes to the development of cell mediated immunity , whereas secretion of interleukin ( IL ) 4 , IL 5 and IL 6 contributes to development of humoral immunity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Northern blot analysis revealed that 2 h after CA 5d , PECs expressed a high level of IL 2 , IFN gamma , IL 1 beta and a low level of IL 10 and TNF alpha mRNAs , while IL 4 and IL 5 mRNAs were absent , suggesting the development of TH 1 subset . ^^^ At 24 h , while IL 2 mRNA remained high , IL 1 beta and IFN gamma expression had decreased and IL 10 and TNF alpha mRNAs were no longer detectable . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This absence of transcription was not modulated by IL 2 , IFN gamma or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We observed a rapid and synergistically augmented release of TNF , and also of IL 1 , IL 2 , IL 4 , IL 6 , and IFN gamma , compared to stimulation of the individual cell types alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma , IL 1 beta , and TNF alpha mRNA increased 3 days after virus inoculation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To clarify the mechanisms regulating TN expression , we studied the effects of selected cytokines ( PDGF , bFGF , TGF beta , IL 1 , IL 4 , IL 6 , IFN gamma , and TNF alpha ) found in fibrotic tissue on TN expression by dermal fibroblasts . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
SPE C raised the mRNA level of type 1 helper T cell ( TH 1 ) related cytokines , such as interferon gamma ( IFN gamma ) , interleukin 2 ( IL 2 ) , and tumor necrosis factor beta ( TNF beta ) . ^^^ Consistent with the mRNA accumulation , protein concentrations of IFN gamma , IL 2 , and TNF were increased in SPE C stimulated PBMCs , but IL 4 was not . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The transcripts for IL 4 , IL 5 , GM CSF , and TNF alpha were expressed in the splenocytes from acutely infected mice not treated with rIL 2 . mRNAs for IL 2 , IFN gamma , IL 6 , IL 10 which were not detected in acutely infected mice could be reversed by administration of rIL 2 in vivo . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We measured the levels of the cytokines IL 1 alpha , IL 1 beta , IL 2 , IL 6 , TNF alpha , and IFN gamma in culture supernatants of stimulated whole blood cells derived from 23 tumor patients undergoing a 4 week oral treatment with a spagyric extract from Echinacea angustifolia , Eupatorium perfoliatum , and Thuja occidentalis ( Echinacea complex ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of IL 2 , IL 4 , IL 5 , TNF alpha , and IFN gamma was mainly detected in lymphocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A myocarditic coxsackievirus B 3 ( CB 3 ) infection in Balb / c mice was used to investigate the effects of 12 weeks of methyl mercury ( MeHg ) exposure ( 3 . 69 mg / g diet ) on inflammatory heart lesions , virus in the heart , the cytokine response , i . e . cachectin / TNF alpha and gamma interferon ( IFN gamma ) levels in plasma , and on disease complications and mortality . ^^^ Plasma levels of both TNF alpha and IFN gamma increased on day 3 of the infection in MeHg treated as well as in non MeHg treated mice , but the mean IFN gamma response was more pronounced in the MeHg treated mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , both IFN gamma and IL 4 , products of Th 1 and Th 2 cells , respectively , synergized with TNF alpha and IL 1 in inducing MCP 4 mRNA accumulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of inflammatory cytokines such as IL 1 , TNF alpha , and IFN gamma by term trophoblast could trigger labor or be a consequence of labor associated events . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We determined the effects of T helper ( Th ) 2 derived cytokines on the induction of iNOS from an epithelial A 549 cell line and human airway epithelial cells stimulated by a mixture of interleukin 1 beta ( IL 1 beta ) , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Only trace amounts of VCAM 1 mRNA or protein were constitutively expressed on dermal fibroblasts but both were rapidly ( within 4 hr ) upregulated by tumour necrosis factor alpha ( TNF alpha ) and interleukin 1 alpha ( IL 1 alpha ) and somewhat slower ( 20 hr ) by interferon gamma ( IFN gamma ) . ^^^ The combination of TNF alpha and IFN gamma had at least an additive effect . ^^^ Adhesion of Jurkat cells to dermal fibroblasts was markedly increased by stimulation of fibroblasts with TNF alpha and IFN gamma ( 22 % of cells adhered versus 9 % on unstimulated fibroblasts ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Growth inhibition of the intracellular mycobacteria such as Mycobacterium tuberculosis , M . bovis , M . kansasii , M . avium , M . intracellulare , M . fortuitum , and M . chelonae subsp . abscessus by interferon gamma ( IFN gamma ) or tumor necrosis factor alpha ( TNF alpha ) treated murine peritoneal macrophages elicited by proteose peptone was studied in vitro . ^^^ Then , macrophages were treated with IFN gamma or TNF alpha at a concentration of 10 to 1000 U / ml for 2 days . ^^^ Macrophages were cultured with or without IFN gamma or TNF alpha for additional day . ^^^ Stimulation with IFN gamma or TNF alpha and subsequent phagocytosis of M . tuberculosis or M . intracellulare increased O 2 production , which was assayed by the method of cytochrome C reduction by murine peritoneal macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
LPS and tumour necrosis factor alpha ( TNF alpha ) , but not interleukin 4 ( IL 4 ) or interferon gamma ( IFN gamma ) , induced a transient expression of E selectin , both when injected in vivo and when added to endothelial cell lines in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Profiles of cytokine expression differed between progressive and regressive Burkitt ' s tumors in that regressive tumors expressed larger levels of TNF alpha , IL 6 , IFN gamma , IP 10 , Mig , and IL 12 p 35 , but not other chemokines / cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neutralizing antibody to TGF beta , but not to IFN gamma or TNF alpha , alleviated the suppression of ISC formation in some of the experiments . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum was tested in bioassays for cytokines known to modulate lymphocyte : endothelial interactions in vivo and in vitro ( IFN gamma , TGF beta , TNF alpha , IL 6 , IL 1 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Similarly , PTX and CsA synergistically inhibited the release of IL 2 , IFN gamma and , to a lesser degree , TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
KODA 408 T cell clone produced a considerable amount of TNF alpha , IFN gamma , and IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Since autoimmune response involves immune activation , the plasma levels of interferon alpha ( IFN alpha ) , interferon gamma ( IFN gamma ) , interleukin 12 ( IL 12 ) , interleukin 6 ( IL 6 ) , tumor necrosis factor alpha ( TNF alpha ) , and soluble intercellular adhesion molecule 1 ( sICAM 1 ) were measured in autistic patients and age matched normal controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULT : Further , a reverse transcription polymerase chain reaction ( RT PCR ) in the MLC was established that detects mRNA of a broad panel of cytokines ( IL 1 beta , IL 2 , IL 4 , IL 6 , IL 9 , IL 10 , TNF alpha , TNF beta , IFN gamma , TGF beta ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 2 , IL 10 and IFN gamma had no effect in this regard , whereas TGF beta 1 partially inhibited hair growth and EGF , TNF alpha and IL 1 beta completely abrogated it . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We also investigated the expression of cytokines ( interferon gamma : IFN gamma ; tumour necrosis factor alpha : TNF alpha ; interleukin 4 : IL 4 ; interleukin 6 : IL 6 ) in the same specimens . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To extend these observations to primary human macrophages , we investigated the anticryptococcal activity of human alveolar macrophages stimulated with interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , or macrophage colony stimulating factor ( M CSF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Before start of perfusion , higher levels of TNF alpha , F1+2 and TAT levels were found in the IFN gamma group . ^^^ Several investigators have reported that interferon gamma ( IFN gamma ) can alter tumor necrosis factor alpha induced effects in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The induction of immunoregulatory cytokines IL 1 beta , IL 6 , IL 12 , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) was studied with neonatal ( cord blood ) monocyte derived macrophages ( MDM ) after in vitro infection with respiratory syncytial virus ( RSV ) . ^^^ Significant increase of expression of mRNA for IL 6 , IL 12 , TNF alpha and IFN gamma occurred within 2 h after infection and decreased within 6 h after infection . ^^^ At 20 h after infection the MDM produced and secreted moderate levels of IL 6 and TNF alpha ; however , no IL 12 and IFN gamma activities were detected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro production of TNF alpha by murine interferon gamma ( IFN gamma ) and lipopolysaccharide ( LPS ) stimulated macrophages was strongly inhibited by co culturing with the whole yeast cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Among various tested cytokines , interferon gamma ( IFN gamma ) and to a lower extent , tumour necrosis factor alpha ( TNF alpha ) were found to upregulate CD 40 expression on fibroblasts . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Raised anti endothelial cell autoantibodies ( AECA ) , but not anti neutrophil cytoplasmic autoantibodies ( ANCA ) , in recurrent oral ulceration : modulation of AECA binding by tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) . ^^^ Stimulation of endothelial cells with TNF alpha significantly increased AECA binding of both ROU ( P < 0 . 005 ) and control samples ( P < 0 . 0001 ) , while IFN gamma decreased binding ( ROU P < 0 . 0001 ; controls P < 0 . 05 ) . ^^^ Moreover , these findings suggest that endothelial cell expression of AECA target antigens is increased by TNF alpha and decreased by IFN gamma stimulation . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
T cells from TNP immune H 2k mice produce interferon gamma ( IFN gamma ) , interleukin 2 ( IL 2 ) , IL 3 , IL 5 , tumor necrosis factor alpha ( TNF alpha ) , IL 10 , and very low levels of IL 4 on reexposure to the specific antigen in vitro . ^^^ By contrast , T cells from H 2d mice produce IL 3 , TNF alpha , IL 10 , and IL 4 but very low levels of IL 2 , IL 5 and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the presence of interferon gamma ( IFN gamma ) , human tumor necrosis factor alpha ( Hu TNF alpha ) , which binds to murine TNF alpha receptor type 1 ( TNF R 1 ) but not to murine TNF R 2 , was effective in inducing nitric oxide ( NO ) production in spleen derived macrophages ( M phi ) , albeit at concentrations 12 . 5 fold greater than those required by murine TNF alpha ( Mu TNF alpha ) , to achieve the same result . ^^^ Addition of anti TNF R 1 completely inhibited the Mu TNF alpha mediated induction of NO , demonstrating that TNF R 1 is critical to the IFN gamma dependent TNF alpha mediated induction of M phi effector function . ^^^ Priming of spleen derived M phi with either IFN gamma or granulocyte macrophage colony stimulating factor ( GM CSF ) heightened the maximal responses to both TNF alpha species and increased the overall effectiveness of Hu TNF alpha without increasing expression of either TNF alpha receptor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , cytokine priming with tumor necrosis factor alpha ( TNF alpha ) , interferon gammma ( IFN gamma ) , or interleukin 1 alpha / beta ( IL 1alpha / beta ) induced an enhanced ICAM 1 expression on noninfected as well as on RSV infected epithelial cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
VD or RA treated U 937 cells acquired resistance against Fas or TNF receptor ( TNFR ) mediated apoptosis , whereas apoptotic cell death was accelerated in IFN gamma treated cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Ex vivo reverse transcriptase PCR of RNA from intraepithelial lymphocytes from control calves showed a cytokine expression pattern consisting of tumor necrosis factor alpha ( TNF alpha ) and gamma interferon ( IFN gamma ) , while intraepithelial lymphocytes from calves with cryptosporidiosis expressed IFN gamma but not TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma up regulates CD 40 expression on cultured keratinocytes , whereas other proinflammatory cytokines , such as IL 1 or TNF alpha , have little effects . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Injection of the superantigen staphylococcal enterotoxin A ( SEA ) to mice rapidly elicits production of the Th 1 related pro inflammatory cytokines IL 2 , TNF , and IFN gamma , while repeated SEA challenges transduce a hyporesponsive state characterized by T cell deletions and anergy in the remaining SEA reactive T cells . ^^^ This coincided with maintained IL 2 production and superinduction of TNF , IFN gamma , and IL 4 . ^^^ Interestingly , administration of a second SEA challenge resulted in high serum levels of IL 10 , but the production of all other studied cytokines ( IL 2 , TNF , IFN gamma , and IL 4 ) was impaired . ^^^ Pretreatment of mice with neutralizing anti lL 10 mAb before the SEA challenges substantially enhanced IFN gamma and TNF serum levels , but failed to rescue IL 2 production . ^^^ We conclude that repeated SEA challenges promote an IL 10 producing Th cell subset ( `` Th 10 ' ' profile ) , which exerts an immunoregulatory effect by suppressing IFN gamma and TNF production . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The addition of TNF alpha in the absence of IFN gamma , however , had no effect on IL 12 production in the T cell dependent pathway . ^^^ Similarly , addition of TNF alpha in the presence or the absence of IFN gamma to cultures of LPS or heat killed Listeria and SAC did not increase IL 12 production , but addition of IFN gamma alone greatly enhanced IL 12 production , consistent with the idea that bacterial stimuli induce significant quantities of endogenous TNF alpha production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The chemotaxis of N B cells can be inhibited by IFN gamma and IL 2 , and augmented by IL 4 and IL 13 , whereas TNF alpha and IL 10 have no influence . ^^^ The chemotaxis of HIV B cells can be inhibited by IFN gamma and IL 2 , and augmented by TNF alpha , IL 4 , and IL 10 , whereas IL 13 has no influence . ^^^ The IL 8R on N B cells can be down regulated by IFN gamma , IL 2 , and TNF alpha ( selectively on IL 8RA ) , and up regulated by IL 4 and IL 13 , whereas IL 10 has no influence . ^^^ The IL 8R on HIV B cells can be down regulated by IFN gamma and IL 2 , and up regulated by TNF alpha , IL 4 , and IL 10 , whereas IL 13 has no influence . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
As expected , endotoxin administration markedly increased circulating tumor necrosis factor ( TNF ) and interferon gamma ( IFN gamma ) and induced organ injury , hypoglycemia , and hyperlipidemia . ^^^ In GH primed rats , endotoxin induced a further increase of serum IFN gamma ( but not TNF ) ; and five out of six of those rats died within 15 h after giving endotoxin . ^^^ Priming rats by GH ( but not by IGF 1 ) induced a further increased response of serum IFN gamma but not TNF to subsequent endotoxin challenge , suggesting that IFN gamma rather than TNF is likely to be involved in this process . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : HNECs and HMMECs were isolated from nasal mucosa and subsequent continuous subcultures and were stimulated either by IL 1 beta or by the combination of tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) . ^^^ RESULTS : After the combined stimulation by TNF alpha and IFN gamma , HNECs and HMMECs dramatically produced RANTES , as previously observed in HUVECs and bronchial epithelial cell line BEAS 2B . ^^^ We also demonstrated that the amount of RANTES induced by TNF alpha and IFN gamma was higher in HNECs and HMMECs obtained from patients with nasal allergy than in those from patients without allergy . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Exposure to the airborne dust also decreased cytokine production by spleen cells , such as interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Several inhibitors 8Br cAMP , interferon gamma ( IFN gamma ) , INF alpha / beta , lipopolysaccharide ( LPS ) , tumor necrosis factor alpha ( TNF alpha ) , and dimethylamiloride suppress CSF 1 stimulated proliferation in murine bone marrow derived macrophages ( BMM ) even when added in the mid to late G 1 phase of the cell cycle . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Filtration is used on demand to further reduce leucocyte contamination of these components . we have monitored the plasma of PCs prepared by the platelet rich plasma method ( PRP ) , the buffy coat method or by apheresis for IL 6 , IL 1 , transforming growth factor beta ( TGF beta ) , TNF and interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study we investigated freshly dissociated neonatal Lewis rat Schwann cells and a SV 40 transfected neonatal rat Schwann cell line ( Schwann cell line ) for production of mRNA for the immunomodulatory cytokines IL 2 , IL 4 , IL 6 , IL 10 , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) employing RT PCR . ^^^ Primary Schwann cells and Schwann cell line were examined following IFN gamma stimulation and were found to express TNF alpha and IL 6 mRNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We examined the ability of human astrocytoma cell lines , as well as primary human and rat astrocytes , to generate a specific member of the intercrine / chemokine family of cytokines , RANTES , when exposed to TNF alpha , IL 1 beta and IFN gamma . ^^^ IFN gamma alone did not induce RANTES production by astrocytes , but it potentiated the effects of either TNF alpha or IL 1 beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Thus the objective of this study is to examine the effect of IFN beta 1b treatment of endothelial cells ( EC ) on lymphocyte EC adhesion , and on the expression of the adhesion molecules ( AM ) ICAM 1 , VCAM and E selectin induced by IFN gamma , TNF alpha , or IL 1 beta . ^^^ IFN beta 1b ( 1 1000 IU / ml ) had minimal effect on basal expression of AM on HUVEC , but AM could be substantially upregulated by IFN gamma , IL 1 beta or TNF alpha which was associated with a parallel increase in lymphocyte EC adhesion . ^^^ The effect of IFN beta 1b on AM expression induced by IFN gamma , IL 1 beta or TNF alpha was slightly additive , and was associated with a modest increase in lymphocyte EC adhesion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using our methods , it was possible to evaluate , in a single synovial tissue specimen obtained by needle biopsy , the relative amounts of mRNAs for 10 cytokines ( TNF alpha , IL 1 beta , IL 2 , IL 4 , IL 6 , IL 10 , IL 12 p 40 , IL 13 , IL 15 , IFN gamma ) and CD 3 delta chain . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum levels of cytokines , including interferon gamma ( IFN gamma ) , tumor necrosis factor alpha , interleukin 1 alpha ( IL 1 alpha ) , IL 2 , IL 4 , and IL 6 , were measured using radioimmunoassay or enzyme linked immunosorbent assay techniques in patients with the localized form and the extensive form ( alopecia universalis ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulus induced secretion of TNF alpha , IFN gamma , IL 12 , and IL 4 was less than that in controls ; IL 10 secretion was similar . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
BACKGROUND : Hyperthermic isolated limb perfusion ( HILP ) with tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and melphalan is associated with a dramatic anti tumor effect in which the neo vascularization of the tumor is supposed to be the major target . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To study the immune response within the subarachnoid space in patients with neurocysticercosis , we measured the cerebrospinal fluid contents of immunoglobulins A , E , G , and M in 38 patients and the contents of the proinflammatory cytokines TNF alpha , IL 1b , IL 6 and IFN gamma in 17 patients . ^^^ A complex function of the immune response within the subarachnoid space was observed : mean values of IgG , IgM , IgE and interleukins 1b and 6 were increased , whereas values of IgA , TNF alpha and IFN gamma were similar to those of controls . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Studies on a patient with angio immunoblastic lymphadenopathy , another disease showing haematopoietic insufficiency , who developed severe aplastic anaemia , showed massive increases in IFN gamma and TNF alpha levels in serum ; IL 6 and GM CSF levels were below the limit of detection . ^^^ These results correlated with an abnormal distribution of CD4+ and CD8+ T lymphocytes in the patient ' s blood and were compatible with the suppressive effects of IFN gamma and TNF alpha on haematopoiesis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine mRNAs detected in lesion biopsies include the transcripts for IL 1 beta , IL 3 GM CSF , TNF alpha and , less reproducibly , IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mononuclear cell activation was shown by expression of IL 2R ( CD 25 , 10 20 % ) and labeling for IL 2 ( approximately 10 % ) , and IFN gamma ( 10 20 % ) , as well as TNF alpha ( > 50 % ) and iNOS ( > 50 % ) , but only low levels of IL 4 or IL 10 ( < 5 % ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We show that B 7 1 and LFA 3 costimulation activate CD8+ T cells to proliferation , cytokine production ( IL 2 , TNF , and IFN gamma ) , and cytotoxicity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The level of nitric oxide production can be enhanced significantly when IFN gamma treatment is combined with other agents ( e . g . , LPS , TNF alpha , IL 2 , etc . ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Regulation of inducible nitric oxide synthase messenger RNA expression and nitric oxide production by lipopolysaccharide in vivo : the roles of macrophages , endogenous IFN gamma , and TNF receptor 1 mediated signaling . ^^^ To evaluate potential roles for macrophages , IFN gamma , and TNF receptor 1 ( TNFR 1 ) in the regulation of LPS induced inducible nitric oxide synthase ( iNOS ) mRNA expression , we used a model of macrophage depletion as well as IFN gamma ( GKO ) and TNFR 1 ( TNFR 1 / ) knockout mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Antral biopsies from all Hp infected patients showed IFN gamma , TNF alpha , and IL 12 , but not IL 4 , mRNA expression , whereas no cytokine mRNA signal was found in the mucosa of controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Evidence for its lack of activating potential includes in vitro failure to stimulate PBMC proliferation either alone or in the presence of nonmitogenic doses of phorbol ester , failure to induce the expression of early and late activation antigens and failure to induce IFN gamma , TNF alpha , IL 6 or IL 2 release . ^^^ Both TNF alpha and IFN gamma patient plasma levels are significantly reduced in T10B9 as compared to OKT 3 therapy . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These findings indicated that apoptosis might be implicated in ADMC with mAb 17 1A , which was augmented by IFN gamma , while , in addition , TNF alpha may also be one of the major mediators of apoptosis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The differences in MHC class 2 expression between ischemic kidneys of PVG and LEW rats were not associated with differences in production of mRNA for IL 2 , IFN gamma , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lipopolysaccharides ( LPS ) , interleukin 1 beta , interferon gamma ( IFN gamma ) , and TNF alpha increase the levels of Fas mRNA in cultured mesangial and tubular cells . ^^^ Increases in Fas by LPS , TNF alpha , and IFN gamma enhance the killing induced by the anti Fas antibody . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Anti TNF alpha Ab treatment stimulated the secretion of interleukin ( IL ) 2 and interferon gamma ( IFN gamma ) in the infected T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The kinetics of cytokine gene expression , including interleukin ( IL ) 1alpha , IL 1beta , IL 2 , IL 6 , IL 10 , tumor necrosis factor , and interferon gamma ( IFN gamma ) , within the grafted kidney of rejector and acceptor animals , were determined using Northern blot hybridization . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cells from IDDM patients ( n = 53 ) produced significantly higher amounts of Th 1 cytokines gamma interferon ( IFN gamma ) ( P = 0 . 028 ) and tumor necrosis factor alpha ( TNF alpha ) ( P = 0 . 007 ) than normal control subjects ( n = 56 ) , while Th 2 cytokine levels ( interleukin [ IL ] 4 , IL 10 ) were similar . ^^^ However , when the balance between Th 1 and Th 2 cytokine production was analyzed in individuals , the ratio between IFN gamma or TNF alpha and IL 4 or IL 10 was clearly biased toward Th 1 reactivity in patients with IDDM ( P = 0 . 0001 ) , while a dominance of Th 2 cytokine production was seen in Graves ' disease ( P = 0 . 0001 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The actions of TNF alpha and IL 6 were inhibited by indomethacin , an inhibitor for prostaglandin synthesis , and by neutralizing anti IFN gamma and anti IL 3 antibodies . rIL 4 , when added at the start of the culture , also inhibited mast cell development induced by rIL 6 and rTNF alpha . ^^^ Nevertheless , neutralizing anti IL 6 and anti TNF alpha antibodies did not suppress mast cell development induced by PGE and IFN gamma . ^^^ TNF alpha and IL 6 enhanced IFN gamma production , but suppressed IL 4 production in the cultures . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 4 ( IL 4 ) , and IL 10 was measured by enzyme linked immunosorbent assay ( ELISA ) , after stimulation of synovial fluid mononuclear cells ( SFMC ) with Borrelia burgdorferi ( Bb ) in the supernatant . ^^^ RESULTS : SFMC produced high amounts of IFN gamma and TNF alpha , but little or no IL 4 , upon stimulation with Bb antigen , indicating a Th 1 type cytokine pattern . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lipopolysaccharide ( LPS ) and interferon gamma ( IFN gamma ) , but not IL 1beta and TNF alpha , induced rat peritoneal macrophages to produce large amounts of nitrite . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
From experiments with neutralizing antibodies to tumour necrosis factor alpha ( TNF alpha ) and IFN alpha / beta it was concluded that the synergistic effect is dependent on autocrine secretion of TNF alpha , which acts as a second signal and synergizes with IFN gamma in NO production . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In a murine model of rickettsial disease in which , as in human rickettsioses , endothelial cells are the major target of infection , depletion of IFN gamma or TNF alpha converts a sublethal infection into a uniformly fatal disease with overwhelming rickettsial growth and decreased nitric oxide ( NO ) synthesis . ^^^ The kinetics of NO production and rickettsial survival and growth were examined on Days 1 , 2 , and 3 after inoculation of endothelial cells with Rickettsia conorii under four different experimental conditions : ( a ) no cytokine treatment , ( b ) treatment with IFN gamma and TNF alpha , ( c ) treatment with cytokines and NG monomethyl L arginine , a competitive inhibitor of NO synthesis , and ( d ) treatment with sodium nitroprusside , a source of NO . ^^^ Endothelial cells were examined for the presence of inducible nitric oxide synthase mRNA by specific reverse transcriptase PCR after stimulation with IFN gamma and TNF alpha . ^^^ Transformed and diploid mouse endothelial cells stimulated by the combination of recombinant murine IFN gamma and TNF alpha killed intracellular Rickettsia conorii by a mechanism that required the synthesis of NO . ^^^ Addition of nitroprusside , which released NO , also exerted a strong antirickettsial effect in the absence of IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the current study we examined the effect of the di catechol rooperol [ ( E ) 1 , 5 bis ( 3 ' , 4 ' dihydroxyphenyl ) pent 4 en 1 yne ] on the process of microvascular endothelial cell ( MME ) activation by TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) , but not interleukin 1 ( IL 1 ) , tumour necrosis factor alpha ( TNF alpha ) or IL 6 , was able to increase the level of H mRNA in both cell lines . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The ability of cytokine synthesis inhibitory factor or interleukin 10 ( IL 10 ) and interferon gamma ( IFN gamma ) to modulate the production of tumor necrosis factor ( TNF alpha ) induced by lipopolysaccharide ( LPS ) was examined in mouse bone marrow derived macrophages ( BMDM ) . ^^^ IFN gamma profoundly enhances LPS stimulated TNF alpha production , whereas IL 10 is markedly inhibitory , demonstrating the opposing effects of IFN gamma and IL 10 on BMDM . ^^^ Early neutralization of endogenously produced , LPS stimulated IL 10 markedly enhanced short term TNF alpha production , an effect further amplified by the absence of IFN gamma priming . ^^^ The regulatory effects of IFN gamma and IL 10 apparently occurred at the translational ( or post translational ) level , with TNF alpha mRNA steady state levels remaining unchanged . ^^^ Furthermore , IFN gamma exerts its enhancing effect on TNF synthesis by the transcriptional inhibition of IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The percentage of polymorphonuclear leukocytes showed a significant increase in B group , while lymphocytes significantly increased in T group with a statistically significant difference . 4 ) Although no significant difference in concentrations of IL 1 beta , IL 2 , IFN gamma and TNF alpha in serum was noticed among the three groups , the average concentrations of IFN gamma and TNF alpha in pleural fluid in T group were significantly higher than those in the other groups . 5 ) TNF alpha mRNA of mononuclear cells in pleural fluid was strongly expressed in 3 out of 11 patients of T group , while no expression was observed in 6 patients of M group . ^^^ In conclusion , the measurement of concentrations of two kinds of cytokines in pleural fluid , IFN gamma and TNF alpha , may be clinically useful for the differential diagnosis of tuberculous pleurisy from parapneumonic pleurisy and malignant pleurisy . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines immunoreactive to monoclonal antibodies ( mAb ) directed to human cytokines ( TNF alpha , IL 1 alpha , IL 2 , IL 6 , IFN gamma ) could be detected by enzyme linked immunosorbent assay ( ELISA ) in the case of primates only . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The authors examined the interleukin 6 concentrations and those of its soluble receptor ( IL 6 , IL 6R ) , of the tumour necrotizing factor alpha ( TNF alpha ) interferon gamma ( IFN gamma ) and of adhesion molecules ICAM 1 and VCAM 1 . ^^^ The most remarkable finding in the homogenate of a peroperative excision were low IL 6 , IL 6R and adhesion molecule values in group A , as compared with B and C and the absence of differences in TNF alpha values between groups and in particular markedly higher IFN gamma values in group B which supports theories on the etiopathogenetic role of this cytokine in GB disease . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Vbeta8 . 1 and Vbeta5 . 2 treatments largely maintained lymphocyte proliferation and IL 2 and IFN gamma release , and prevented excessive IL 6 , IL 10 , and TNF alpha secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Among various cytokines possibly induced by OK 432 and BCG , IL 1 alpha , TNF alpha and lymphotoxin significantly improved FBG and GTT in KK Ay mice , whereas IL 2 and IFN gamma did not . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Thus , IFN gamma and IL 1beta levels were increased 13 fold and 30 fold , respectively , with more modest increases in IL 6 levels ( 5 fold ) and TNF levels ( 2 fold ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
LCH lesions and normal LCs were studied for the presence of cytokines known to influence the functioning of LCs : IL 1 alpha , IL 1 beta , IL 4 , GM CSF , IFN gamma , TGF alpha , TGF beta , bFGF , and TNF alpha . ^^^ Cytokines were abundantly present within LCH lesions ; LCH cells stained for IL 1 alpha , IL 1 beta , IL 4 , GM CSF , TGF alpha , TGF beta , TNF alpha , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To clarify the immunological mechanisms in more detail , the expression of vascular cell adhesion molecular 1 ( VCAM 1 ) has been examined in the heart of acute myocarditis and on cultured cardiac myocytes treated with interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) . ^^^ VCAM 1 expression was also induced on cultured cardiac myocytes by treatment with IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this cascade , interleukin ( IL ) 1 , IL 2 , IL 6 , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) produced by donor T cells and monocytes / macrophages play a major effector role . ^^^ Levels of IL 1beta , IL 2 , IL 3 , IL 4 , IL 6 , TNF alpha , IFN gamma , and granulocyte macrophage colony stimulating factor ( GM CSF ) were measured in the supernatants after various times of incubation . ^^^ On the other hand , we found markedly lower levels of IFN gamma , TNF alpha , and IL 4 in cord blood throughout the incubation period . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The soluble factors were able to induce TNF alpha in the macrophages and to potentiate the TNF alpha release induced by IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study examined the effect of the pro inflammatory cytokines interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) on the induction of MHC class 1 related genes in functionally mature brain neurons derived from cultures of dissociated rat hippocampal tissue . ^^^ All neurons transcribed the alpha chain of the interferon type 2 receptor ( binding IFN gamma ) along with the p 55 receptor for TNF alpha . ^^^ In contrast to IFN gamma , treatment with TNF alpha did neither upregulate TAP1 / TAP2 nor beta 2 microglobulin gene expression , but induced MHC class 1 heavy chain gene transcription in all neurons . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast to monocytes the expression profile on HMC 1 did not change upon stimulation with IL 4 , TNF alpha , IFN gamma , PMA or salbutamol . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The identified till now cytokines induced by PRP were IFN gamma and TNF alpha but many other cytokines may be stimulated also . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The use of 2 CdA either with IFN alpha or IFN gamma and 2 CdA with TNF alpha was found to inhibit , in a dose dependent manner , the growth of colonies formed by hematopoietic precursor cells from CML and AML patients as well as from normal individuals , with the greatest effect being observed after the use of 2 CdA and IFN alpha at their highest concentrations . . ^^^ The objective of the present study was to investigate the interactions of 2 chlorodeoxyadenosine ( 2 CdA ) with interferon alpha or gamma ( IFN alpha , IFN gamma ) , as well as between 2 CdA and recombinant human tumor necrosis factor alpha ( rhTNF alpha ) , on the clonal growth of granulocyte macrophage progenitor cells ( CFU GM ) from patients with chronic myeloid leukemia ( CML ) and on clonogenic leukemia blasts ( CFU L ) from acute myeloid leukemia ( AML ) patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Levels of the proinflammatory cytokines tumor necrosis factor alpha ( TNF alpha ) and gamma interferon ( IFN gamma ) in tissues temporarily increased at around weeks 2 to 4 after infection , rapidly decreased thereafter , and returned to normal by week 8 . ^^^ Splenocytes and splenic M phi s of MAC infected mice ( week 2 ) produced and / or released into the culture fluid significant amounts of TNF alpha ( in a cell bound form ) , IFN gamma , and IL 10 , but not TGF beta , during 3 days of cultivation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The proliferative response and production of GM CSF , TNF alpha , and IFN gamma by C 27 were further augmented by replacing the possible first DR anchor 4Tyr of the mutated Ras peptide with Trp , a more potent anchor residue for the DR 1 molecule . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Before IFN treatment , IL 2 , TNF beta , IFN gamma and IL 8 mRNA were associated with severe hepatitis activity whereas IL 4 mRNA was associated with weak hepatitis activity , regardless of IFN response . ^^^ IL 2 , TNF beta and IFN gamma mRNAs were significantly greater in IFN non responders . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Culture supernatants of infected and uninfected PBMCs from HTLV 1 carriers exhibited markedly increased levels of interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) compared with the HTLV 1 seronegative controls . ^^^ In the HTLV 1 carriers , IFN gamma was produced by the CD4+ lymphocytes , whereas TNF alpha was secreted by the monocytes . ^^^ Addition of anti IFN gamma or anti TNF alpha antibodies to the HTLV 1 carrier PBMC cultures diminished the inhibition of intracellular growth of L . pneumophila . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using flow cytometry for the detection of intracellular cytokine expression , we demonstrate in the present study that large numbers of CD4+ and CD8+ TCR alphabeta+ T cells from the intraepithelial region and lamina propria of the colon of diseased , but not from disease free mice , produced interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^ Large numbers of T cells from peripheral lymphoid tissues of these animals also expressed IFN gamma and TNF alpha , but few expressed interleukin 4 , demonstrating a strong bias towards Th 1 type T cell responses in these animals . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma alone had a modest effect on IP 10 mRNA accumulation , whereas tumor necrosis factor alpha ( TNF alpha ) , yeast particles opsonized with IgG ( Y IgG ) , lipopolysaccharide ( LPS ) , and formyl methionyl leucyl phenylalanine ( fMLP ) all failed to up regulate IP 10 gene expression . ^^^ However , stimulation of neutrophils with IFN gamma in combination with either TNF alpha or LPS ( but not with Y IgG or fMLP ) resulted in a considerable induction of IP 10 mRNA transcripts , as well as in the extracellular release of the protein . ^^^ Furthermore , mRNA stabilization analyses demonstrated that IP 10 mRNA isolated from PMN stimulated with IFN gamma only , or with IFN gamma plus either TNF alpha or LPS , had similar half lives . ^^^ Finally , we found that interleukin 10 , a known inhibitor of chemokine production in PMN , moderately suppressed the extracellular production of IP 10 in neutrophils stimulated with IFN gamma plus either LPS or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Coculture of irradiated autologous tumor cells with OvCTL induced secretion of IFN gamma , GM CSF and TNF alpha , but not IL 4 , indicating a T helper 1 type response . ^^^ Finally , the Her2 / neu peptide p 654 662 , earlier identified as a tumor antigen in both ovarian and breast cancer , induced cytotoxicity as well as the specific release of IFN gamma and TNF alpha but not IL 4 by OvCTL and BrCTL . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor derived recognition factor ( TDRF ) induces production of TNF alpha by murine macrophages , but requires synergy with IFN gamma alone or in combination with IL 2 to induce nitric oxide synthase . ^^^ A constitutively produced soluble activity , designated tumor derived recognition factor ( TDRF ) , from L 1210 , P 815 and EL 4 tumor targets , was previously shown to synergize with interferon gamma ( IFN gamma ) and subactivating concentrations of interleukin 2 ( IL 2 ) to induce murine macrophage production of tumor necrosis factor alpha ( TNF alpha ) and nitric oxide ( NO ) for cytotoxicity of the target of origin . ^^^ Another study had suggested that TDRF upregulated both TNF alpha receptor ( TNF alpha R ) and IFN gamma receptor ( IFN gamma R ) mRNA synthesis , as well as increased TNF alpha and IFN gamma binding to their receptors . ^^^ At lower concentrations , TDRF synergized with either IFN gamma alone or in combination with IL 2 to stimulate a dose related increase in the expression of TNF alpha mRNA and secretion of TNF alpha , as well as increased induction of NOS mRNA and cytotoxic NO generation by macrophage . ^^^ Taken together , our results indicate that TDRF acted independently in a dose dependent fashion to induce macrophage synthesis and release of TNF alpha , but in the absence of IFN gamma or in combination with IL 2 failed to induce the NOS enzyme which was necessary for cytotoxic NO generation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Transforming growth factor beta , which inhibits cytokine induced NK cell production of IFN gamma and TNF alpha , also decreased cytokine induced NK cell apoptosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
While this cytokine is definitely linked to sepsis and tumor associated weight loss in some experimental models , other cytokines , such as interleukin 6 ( IL 6 ) or interferon gamma ( IFN gamma ) , alone or in combination with TNF , may also play an important role in the development of cachexia . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Culture of Mphi in the presence of IFN gamma or TNF alpha for 24 h before infection did not augment the fungistatic activity of collagen adherent Mphi . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
While TNF alpha , but not IFN gamma , increased RANTES mRNA expression and protein release , the combination of TNF alpha and IFN gamma caused a greater degree of expression and release in a time and dose dependent manner . ^^^ Sequential treatment of airway smooth muscle cells with TNF alpha and IFN gamma showed that IFN gamma sensitized the cells to the stimulatory effect of TNF alpha . ^^^ Using a modified Boyden chamber technique , RANTES separated by reverse phase liquid chromatography from cell culture supernatants of airway smooth muscle cells stimulated by TNF alpha and IFN gamma showed a strong chemoattractant effect on human eosinophils , an effect inhibited by an anti RANTES Ab . ^^^ RANTES production induced by TNF alpha and IFN gamma was inhibited partly by the Th 2 derived cytokines , IL 4 , IL 10 , and IL 13 , as well as by dexamethasone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inflammatory cytokines including TNF alpha , IL 1beta , and IFN gamma are increased in sera and lesions of Kaposi ' s sarcoma ( KS ) patients . ^^^ Production and release of bFGF is induced in a synergistic fashion by TNF alpha , IL 1beta , and IFN gamma , and its release is further promoted by low cell density and by the serine proteases plasmin and thrombin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In in vivo studies , ALPS patients manifested greatly increased circulating levels of IL 10 ( > 100 fold ) , compared with both healthy individuals and various disease controls ; in contrast , their levels of IL 1 beta , IL 4 , and IFN gamma were normal and their levels of IL 2 and TNF alpha were marginally increased . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 6 and tumour necrosis factor alpha ( TNF alpha ) had a biphasic effect on the Fc alphaR mRNA level ; at 48 h , IL 6 increased steady state mRNA levels about six fold relative to control , TNF alpha increased mRNA four fold , and interferon gamma ( IFN gamma ) induced Fc alphaR mRNA two fold . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The hepatic mRNA expression of other cytokines ( IFN gamma , tumor necrosis factor alpha [ TNF alpha ] and IL 4 ) , as well as humoral and cytokine responses in sera , were not significantly different between control monoclonal antibody ( CmAb ) and anti NK1 . 1 treated mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After intraperitoneal infection with bradyzoites of the Beverley strain of T . gondii , mRNA encoding IFN gamma and TNF alpha was detected in the peritoneal gamma delta T cells by RT PCR technique , and macrophages that produced nitric oxide ( NO ) and expressed HSP 65 were also detected . ^^^ This expression was completely suppressed when mice were simultaneously treated with anti IFN gamma and anti TNF alpha although either antibody alone was less effective . ^^^ The synergistic effect of these cytokines was also demonstrated by an in vitro experiment , in which peritoneal macrophages were cultured with recombinant IFN gamma and TNF alpha . ^^^ These results indicate that gamma delta T cells , which protect against infection with T . gondii induce the expression of HSP 65 by secreting IFN gamma and TNF alpha and the production of NO , and that the expression of HSP 65 is independent of inflammatory chemical compounds like NO and H2O2 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , by monitoring the perfusates from limbs treated with melphalan alone or with melphalan plus tumor necrosis factor ( TNF ) and interferon gamma ( IFN gamma ) , we were able to distinguish the effect of the cytokines on the observed coagulant and fibrinolytic responses . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increase in cytokine production ( IL 1 beta , IL 6 , TNF alpha but not IFN gamma , GM CSF or LIF ) by stimulated whole blood cells in postmenopausal osteoporosis . ^^^ We sought here to determine whether production of IL 1 beta , IL 6 , TNF alpha , IFN gamma , GM CSF and LIF , after direct stimulation in whole blood , was different in healthy ( n = 30 ) or osteoporotic postmenopausal women ( n = 24 ) and whether lumbar bone density ( 1 BMD ) correlated with the values of cytokine production observed in these conditions . ^^^ In the whole population , statistically significant negative correlations were observed between lumbar BMD and IL 1 beta ( r = 0 . 46 ) ( p < 0 . 0005 ) , IL 6 ( r = 0 . 50 ) ( p < 0 . 0001 ) and TNF alpha ( r = 0 . 39 ) ( p < 0 . 005 ) production while no such correlations were observed for IFN gamma , GM CSF or LIF . ^^^ In conclusion , the study of cytokine production by immune cells cultured in autologous whole blood suggests that in women more than 10 years past the menopause and presenting a decrease in lumbar bone density corresponding to the new WHO definition of `` osteoporosis ' , production of IL 1 beta , IL 6 and TNF alpha is still increased compared to controls matched for age and ovarian function , while no differences are reported for IFN gamma , GM CSF or LIF production . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The potential pathological effects of IFN gamma during the delayed type of hypersensitivity can be related to its ability to synergise with other inflammatory cytokines such as TNF alpha , IL 1 beta , and others . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , in vitro stimulation of both IL 10 deficient peritoneal exudate cells and spleen cells with heat killed Listeria resulted in a dramatically enhanced proinflammatory cytokine response ( e . g . , IL 12 , IFN gamma , TNF alpha , IL 1alpha , and IL 6 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
ProMMP 9 secretion by CD3+ and CD4+ , but not by CD8+ T cells was augmented in response to TNF alpha and IL 1 , and down regulated by IFN gamma , while macrophage inflammatory protein ( MIP ) 1alpha , MIP 1beta , and RANTES ( regulated upon activation and normally T cell expressed and secreted ) ( beta chemokines ) up regulated the secretion of proMMP 9 by all of the lymphocyte subsets tested . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The release of tumor necrosis factor alpha ( TNF alpha ) by interferon gamma ( IFN gamma ) induced THP 1 cells stimulated with smooth lipopolysaccharide is inhibited by MAbs against HLA DR and CD 14 receptors on the effector cell . ^^^ This undifferentiated cell line did not respond to LPS in terms of TNF alpha release , but when induced with 250 U / ml of IFN gamma for 48 h , the cells released TNF alpha ( 174 + / 58 . 6 U / ml . ^^^ It seems therefore , that HLA DR receptor may be with CD 14 in triggering TNF alpha release by IFN gamma , induced THP 1 cells . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The production of IFN gamma is induced by IL 12 with tumor necrosis factor alpha ( TNF alpha ) as a costimulator and inhibited by IL 10 . ^^^ Detectable CSF levels of IFN gamma correlated positively with IL 12 p 40 ( r = 0 . 40 , P = 0 . 02 ) and TNF alpha ( r = 0 . 46 , P = 0 . 04 ) but not with IL 6 , IL 8 , or IL 10 . ^^^ In contrast to CSF levels of TNF alpha , IL 12 , and IL 10 , those of IFN gamma were significantly higher in patients with pneumococcal meningitis than in children with meningitis caused by Haemophilus influenzae and Neisseria meningitidis , presumably because of a high CSF TNF alpha / IL 10 ratio in the former . ^^^ We suggest that IL 12 and TNF alpha induced IFN gamma production may contribute to the natural immunity against microorganisms in the CSF compartment during the acute phase of bacterial meningitis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 12 treatment also resulted in an increase in mRNA expression and protein production for IFN gamma , tumor necrosis factor alpha ( TNF alpha ) , and nitric oxide from spleen cells . ^^^ Moreover , combined treatment resulted in an increase in both IFN gamma and TNF alpha production , as well as in a substantial reduction in H . capsulatum burden at 35 and 90 days postinfection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Since experimental studies have shown that tumour necrosis factor alpha ( TNF alpha ) has potent anti tumour activity that can be potentiated with cytokines , we tested the efficacy of TNF alpha with interferon gamma ( IFN gamma ) on different human breast cancer cell lines , particularly comparing hormone dependent and independent phenotypes . ^^^ In contrast , the growth of hormone independent cells MDA MB 231 and HS578T was not affected by TNF alpha alone , but a synergistic inhibition was observed when using IFN gamma and TNF alpha together . ^^^ In MDA MB 231 cells this mRNA was increased only in the presence of both TNF alpha and IFN gamma , without a change in the number of surface TNF receptors . ^^^ These findings indicate that TNF alpha treatment in combination with IFN gamma may provide a successful approach to overcome the cellular heterogeneity of advanced breast tumours . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The mRNA of the related inflammatory cytokines TNF alpha and IFN gamma were also expressed in nasal mucosa with stimulation of these reagents . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We show that in two cell lines of human squamous cell carcinoma ( SCC ) which slightly express intercellular adhesion molecule 1 ( ICAM 1 ) , the expression is enhanced not only by interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) but also by interleukin 1 ( IL 1 ) and lipopolysaccharide ( LPS ) . ^^^ This evidence contrasts with previously reported findings indicating that , in human cultured keratinocytes , ICAM 1 expression is induced by IFN gamma and TNF alpha , and not by either IL 1 or LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In some experiments , CM from CD 3 mAb stimulated T lymphocytes was preincubated with neutralizing anti ( alpha ) tumor necrosis factor ( TNF ) , alpha interferon gamma ( IFN gamma ) , or alpha interleukin 1 ( IL 1 ) mAb ( control ) to determine the contributions of each of these cytokines to HRPE chemokine induction by stimulated T lymphocyte CM . ^^^ HRPE cells were stimulated for 8 and 24 hours with IL 1 beta ( 0 . 2 to 20 . 0 ng / ml ) ( positive control ) , TNF alpha ( 0 . 2 to 20 . 0 ng / ml ) ( positive control ) , IFN gamma ( 1 to 1000 U / ml ) , IFN gamma + IL 1 beta , IFN gamma + TNF alpha . ^^^ Interleukin 2 ( IL 2 ; 100 ng / ml ) alone or in combination with IL 1 beta , TNF alpha , or IFN gamma also was tested . ^^^ CM preincubated with neutralizing alpha TNF or alpha IFN gamma mAb induced significantly less HRPE IL 8 and MCP 1 , whereas preincubation of CM with neutralizing alpha IL 1 mAb failed to inhibit CM induced IL 8 or MCP 1 . ^^^ IFN gamma induced dose dependent increases in HRPE MCP 1 , but not IL 8 , IFN gamma potentiated IL 1 beta and TNF alpha induced MCP 1 production , but showed little modulation of IL 1 beta and TNF alpha induced IL 8 production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
According to further experimental results , the carcinoma derived , CD 40 inducing factor ( s ) is not represented within a variety of pleiotropic cytokines including IFN gamma , interleukin 1 , interleukin 6 , and tumor necrosis factor alpha , or common angiogenic factors such as basic fibroblast growth factor , vascular endothelial cell growth factor , angiogenin , and erythropoietin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TIL obtained from colorectal cancer lesions can be efficiently expanded in the presence of IL 7 , some ( 3 / 9 ) of which appear to exhibit autologous tumour recognition as measured by cytolytic effector functions and by detection of IFN gamma and TNF alpha release . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
First , it was shown that THP 1 cells could be induced to secrete significant amounts of TNF alpha by interleukin 1 , lipopolysaccharide , interferon gamma ( IFN gamma ) and PMA alone or in combination with each other . ^^^ Next it was shown that A beta ( 1 40 ) could also induce secretion of TNF alpha by THP 1 cells , but the effect was diminished when this peptide was applied in combination with IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
LDA11 upon stimulation with various inflammatory agents , including IL 1 alpha , IFN gamma , TNF alpha , or LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Other cytokines such as interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , TNF beta / LT , IL 2 , and IL 6 were expressed in a few cases , and IL 1alpha and IL 4 expression were not detected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma , IL 1beta , TNF alpha , IL 6 or IL 10 had no effect on induction of killer activity by IL 15 at the optimal concentration . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Intracerebral expression of the cytokines interleukin ( IL ) 1 beta , tumor necrosis factor alpha ( TNF alpha ) , IL 6 , and interferon gamma ( IFN gamma ) was studied by in situ hybridization and immunohistochemistry . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Hence , we have looked at the release of the cytokine granulocyte macrophage colony stimulating factor ( GM CSF ) in response to bacterial lipopolysaccharide ( LPS ) and the pro inflammatory cytokines interleukin 1 beta ( IL 1 beta ) , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) . ^^^ However , HASM cells exposed to IL 1 beta , TNF alpha and IFN gamma generated more GM CSF than that evoked by any cytokine alone ( 2 . 2 + / 0 . 15 ng ml 1 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study we examined the effects of interleukin 1 beta ( IL 1 beta ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) on progesterone and prostaglandin ( PGE 2 , PGF 2 alpha ) production by primate luteal cells in vitro . ^^^ Approximately 50 , 000 viable luteal cells per tube were incubated in Ham ' s F 10 medium with increasing concentrations of IL 1 beta ( 0 . 1 10 ng / ml ) , TNF alpha ( 1 100 ng / ml ) , or IFN gamma ( 10 1000 U / ml ) in the presence and absence of hCG for 8 h at 37 degrees C . ^^^ TNF alpha and IFN gamma had no effect on progesterone PGE 2 , or PGF 2 alpha production during any phase of the cycle at the doses tested . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Incubation of astroglia with LPS ( 100 ng / ml ; 24 h ) and / or combinations of interleukin 1 beta ( IL 1 beta ; 10 ng / ml , 24 h ) , interferon gamma ( IFN gamma ; 100 U / ml , 24 h ) , or tumor necrosis factor alpha ( TNF alpha ; 100 ng / ml , 24 h ) resulted in significant increases of iNOS mRNA , iNOS protein , and NO production , with the latter indicated by increased nitrite accumulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , interferon gamma ( IFN gamma ) and / or tumor necrosis factor alpha ( TNF alpha ) induced dose dependent expression of both Fas mRNA and Fas protein on the surface of CD34+ cells after 48 hours of serum free culture . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These results demonstrated that NO is an important mediator for the non specific antilisterial activity induced by viable BCG , and IFN gamma , IL 1 alpha and TNF alpha may play a critical role in the non specific antilisterial activity . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of RANTES mRNA and secretion of RANTES protein in supernatants was investigated after stimulation with 50 ng / ml Tumour Necrosis Factor alpha ( TNF alpha ) , interleukin 1 beta ( IL 1 beta ) , interferon g ( IFN gamma ) , lipopolysaccharide ( LPS ) , phorbolymyristate acetate ( PMA ) and serum free medium ( SFM ) for 24 h . ^^^ Cultivated nasal fibroblasts either expressed RANTES mRNA or secreted RANTES protein upon TNF alpha , IL 1 beta and IFN gamma stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Total RNA was prepared from biopsy specimens with the acid guanidinium thiocyanate phenol chloroform method . cDNA was synthesized by M MLV RTase and amplified using the oligonucleotide primers specific for interleukin 1 beta ( IL 1beta ) , IL 2 , IL 3 , IL 4 , IL 5 , IL 6 , IL 7 , IL 8 , IL 9 , IL 10 , tumor necrosis factor alpha ( TNF alpha ) , interferon alpha ( IFN alpha ) , IFN beta , and IFN gamma by PCR methods . ^^^ RESULTS : Although IL 1 beta and IFN gamma mRNA were detected in most specimens , IL 2 , IL 3 , IL 4 , IL 5 , IL 9 , TNF alpha , and IFN beta mRNA were not detected at all . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A splenic APC reduced IFN gamma and TNF alpha production by established Th 1 MBP specific Ak restricted B10 . ^^^ BR APC differ in their ability to stimulate IFN gamma and TNF alpha production by mature Th 1 cells and also influence their Th1 / Th2 commitment in vivo . ^^^ A APC on IFN gamma and TNF alpha production was explored . 2 hour supernatants from antigen activated B10 . ^^^ A APC activated by LPS had the same inhibitory effects on IFN gamma and TNF alpha production by B10 . ^^^ A APC are independent of TNF alpha , IL 4 , IL 10 , IL 12p40 , IFN gamma , IL 13 , TGF beta , and PGE 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although cytokine like regulators , other than IL 1alpha , TNF alpha , or IFN gamma , produced by REC may also modulate the expression of Thy 1 , it is at least in part mediated by IL 1alpha in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Linomide also suppressed the mRNA expression of the Th 1 cytokines IFN gamma , IL 12 and TNF alpha as well as the Th 2 cytokines IL 4 and IL 10 , which are important in the immunopathogenesis of EAMG by promoting antibody production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To clarify if pentoxifylline ( PTX ) may have therapeutic potential for human T cell lymphotropic virus type 1 ( HTLV 1 ) associated myelopathy ( HAM ) , we investigated the in vitro effect of PTX on spontaneous proliferation of peripheral blood lymphocytes ( SPP ) , as well as on the expression of adhesion molecules , such as lymphocyte function antigen 1 ( LFA 1 ) and very late activation antigen 4 ( VLA 4 ) , and the production of inflammatory cytokines , such as tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) and granulocyte monocyte colony stimulating factor ( GM CSF ) , in cultured PBMC from 10 HAM patients , compared with control subjects . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To clarify the roles of cytokines in EAE , we examined levels of interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , transforming growth factor beta 1 ( TGF beta 1 ) and interleukin 10 ( IL 10 ) mRNA in isolates from infiltrating inflammatory cells in EAE lesions induced in Lewis rats . ^^^ Levels of both IFN gamma and TNF alpha mRNA were increased at the early stage of EAE and rapidly decreased at the peak stage . ^^^ Taken together , these results suggest that IFN gamma and TNF alpha might play a crucial role in the development of EAE . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The addition of rhIL 12 led to the generation of Th 1 like cells , which secreted high levels of IL 2 , IFN gamma , TNF alpha , and upon multiple stimulations , significant levels of IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Granulocyte macrophage colony stimulating factor and IFN gamma restore the systemic TNF alpha response to endotoxin in lipopolysaccharide desensitized mice . ^^^ The influence of granulocyte macrophage colony stimulating factor ( GM CSF ) and IFN gamma on the restoration of impaired TNF alpha release in LPS desensitized mice or their refractory macrophages was investigated . ^^^ Mice pretreated with GM CSF or IFN gamma ( 50 microg / kg i . v . ) and injected with 3 mg / kg LPS i . p . displayed increased plasma TNF alpha levels compared with LPS controls . ^^^ Pretreatment of such mice with GM CSF or IFN gamma restored the previously impaired TNF alpha response . ^^^ In cultures of murine monocyte / macrophage containing cell populations , i . e . , alveolar , peritoneal , spleen , bone marrow cells , or blood , the presence of GM CSF or IFN gamma ( 10 ng / ml ) resulted in an enhanced release of TNF alpha initiated by 1 microg / ml LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 13 protects mice from lipopolysaccharide induced lethal endotoxemia : correlation with down modulation of TNF alpha , IFN gamma , and IL 12 production . ^^^ The protective effect of IL 13 was correlated with significant decreases in the production of the inflammatory mediators TNF alpha , IFN gamma , and IL 12 as well as a decrease in the anti inflammatory mediator IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We wondered whether granulocyte macrophage colony stimulating factor ( GM CSF ) , IL 12 , and IFN gamma can prevent or reverse this down regulation of TNF alpha and IL 10 production . ^^^ The LPS induced TNF alpha amounts in desensitized PBMC treated with GM CSF , IFN gamma , or IL 12 and in naive , non cytokine primed cultures were similar , while much more TNF alpha was induced in cytokine primed naive cells . ^^^ Treatment of LPS desensitized pure monocytes with IFN gamma or GM CSF resulted in a very high TNF alpha expression and no difference to cytokine primed naive monocytes was evident any longer . ^^^ IFN gamma and GM CSF prevented and reversed down regulation of TNF alpha and IL 10 synthesis also in the model of IL 10 / TGF beta 1 induced LPS hyporesponsiveness , while IL 12 was ineffective because of its obvious inability to induce IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine expression , determined by semiquantitative PCR , revealed increased expression of IFN gamma , IL 10 , and TGF beta , which was associated with protection from EAE , and reduced TNF alpha , associated with the development of EAE . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found that double immunostaining using immunoalkaline phosphatase ( Vector blue ) for cytokines [ interferon gamma ( IFN gamma ) , interleukin ( IL ) 2 , 4 , 1alpha , 1beta , and 6 , tumor necrosis factor beta ( TNF beta ) , and TNF alpha ] and immunoperoxidase [ diaminobenzidine ( DAB ) ] for cell surface markers ( CD 3 , CD 4 , CD 8 , CD 20 , and CD 68 ) provided the best distinction of double positive cells from single positive or negative cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Importantly , we demonstrated that although IFN gamma , tumor necrosis factor alpha , and IFN alpha / beta mRNA are induced in the liver and kidney after IL 12 administration , the antiviral effect of IL 12 is mediated principally by its ability to induce IFN gamma production in this model . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunosuppressive activity of sera from patients with colorectal and gynecological carcinomas as evaluated by impaired IFN gamma , IL 1 alpha and TNF alpha production of human peripheral mononuclear cells . ^^^ ISA was measured by determining the changes in the production of IFN gamma , IL 1 alpha and TNF alpha by human peripheral mononuclear cells ( PBMC ) from six normal donors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Coculture of NK cells with B lymphocytes resulted in induction of mRNA for IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The accumulation of leukocytes , ovine serum albumin and the cytokines interleukin 1 beta ( IL 1 beta ) , tumour necrosis factor alpha ( TNF alpha ) , interleukin 8 ( IL 8 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) and interferon gamma ( IFN gamma ) was studied during endotoxin induced inflammation in lactating and dry ovine udders , and in the teat cisterns of dry ewes after surgical closure of the passage between the teat and udder cisterns . ^^^ A role was indicated for TNF alpha , IL 8 and GM CSF , but not for IL 1 beta and IFN gamma , during endotoxin induced inflammation in the ovine udder . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Further , we searched for evidence of clusterin synthesis in human umbilical vein endothelial cells ( EC ) and whether this synthesis was regulated by various proinflammatory cytokines ( IL 1 , IL 6 , and TNF ) and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Incubation of C 6 astrocytoma cells with bacterial endotoxin ( lipopolysaccharide ; LPS ) plus interferon gamma ( IFN gamma ) , or with a combination of cytokines ( TNF alpha , IL 1 beta , and IFN gamma ) leads to high levels of inducible nitric oxide synthase ( iNOS ) expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Effects of IFN gamma , TNF alpha and EGF on the expression of HLA class 1 antigen and the proliferation of human hepatocellular carcinoma HepG 2 cells . ^^^ In this study , the effects of IFN gamma , TNF alpha and EGF on the expression of HLA class 1 antigen and the proliferation of human hepatocellular carcinoma HepG 2 cells were investigated . ^^^ In response to IFN gamma or TNF alpha stimulation , the expression of HLA class 1 mRNA in HepG 2 cells was increased by 2 4 fold . ^^^ IFN gamma and TNF alpha could also inhibit the proliferation of HepG 2 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tissue cryostat sections of undecalcified whole paws were analysed for the presence of tumour necrosis factor alpha ( TNF alpha ) , IL 6 , IL 2 , IL 4 , IL 5 interferon gamma ( IFN gamma ) , transforming growth factor beta 2 ( TGF beta 2 ) and TGF beta 3 . ^^^ Interestingly , IFN gamma was not present in the late phase of CIA , despite the continued presence of TNF alpha and IL 6 in the pannus . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There was a progressive increase in the number of TNF alpha , IL 1beta and IL 6 positive cells from day 1 to day 10 of arthritis , during which time IFN gamma production by CD4+ T cells from draining lymph nodes declined sharply . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The unstimulated and induced production of granulocyte macrophage colony stimulating factor ( GM CSF ) , granulocyte colony stimulating factor ( G CSF ) , IL 3 , IL 6 , stem cell factor ( SCF ) , IL 1beta , tumour necrosis factor alpha ( TNF alpha ) , TNF beta , interferon gamma ( IFN gamma ) and transforming growth factor beta ( TGF beta ) was determined after culture of blood mononuclear cells from 22 patients with severe beta thalassaemia in a regular transfusion programme , five non regularly transfused patients with beta thalassaemia intermedia and nine normal persons . ^^^ A distinct pattern of cytokine production in thalassaemic patients was detected , namely a low unstimulated production of all cytokines and a significant increase in the stimulated production of IFN gamma , TNF alpha and IL 1beta ; these abnormalities were more pronounced in the more heavily transfused older patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using a semiquantitative method of PCR , we found that the expression of C 3 is up regulated by the cytokine tumor necrosis factor alpha ( TNF alpha ) , but not by the cytokines interferon gamma ( IFN gamma ) and interleukin 1 alpha ( IL 1 alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokines IL 1 beta , IL 4 , IL 6 , TNF alpha , TGF beta 2 , IFN gamma and IFN alpha were assessed for their ability to modulate the expression of cell surface antigens . ^^^ MHC class 1 and class 2 antigen expression was unregulated by IFN gamma , IFN alpha and / or TNF alpha in cell lines established from primary melanoma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , the expression of IL 1 beta , TNF alpha , and IL 6 on the IFN gamma stimulated HSG cells was detected by immunohistochemistry and RT PCR analysis . ^^^ These results indicate that human salivary gland cells can be induced to express HLA DR mRNA by IFN gamma concomitant with inflammatory cytokine gene expressions such as IL 1 beta , TNF alpha , and IL 6 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using two independent PCR based quantification techniques , we have determined the levels of IL 1 beta , IL 2 , IL 4 , IL 6 , IL 10 , IFN gamma and TNF alpha mRNA in multiple sclerosis patients at the moment of diagnosis of the disease and prior to any immunosuppressive treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Enhancement of antibacterial activity of clofazimine against Mycobacterium avium Mycobacterium intracellulare complex infection induced by IFN gamma is mediated by TNF alpha . ^^^ The effects of interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) alone or in combination with free or liposomal clofazimine against Mycobacterium avium Mycobacterium intracellulare complex ( MAC ) were investigated . ^^^ Treatment of murine resident peritoneal macrophages with 50 U / mL IFN gamma ( pre infection ) or 30 U / mL TNF alpha ( post infection ) , caused significant reduction in intracellular MAC growth ; this response was suppressed by anti TNF alpha antibodies or pentoxifylline . ^^^ Activation of macrophages with IFN gamma or TNF alpha enhanced the intracellular activities of free and liposomal clofazimine against MAC ; the individual antimycobacterial activities of free and liposomal clofazimine , however , were comparable . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The duration of pretreatment with TGF beta 1 required to inhibit MC TNF alpha release was similar to that required for downregulation of MC TNF alpha dependent cytotoxicity by IFN gamma . ^^^ TGF beta 1 and IFN gamma had an additive inhibition on TNF alpha release by PMC . ^^^ This inhibitory effect was abrogated and TNF alpha dependent cytotoxicity was enhanced by the addition of anti TGF beta 1 antibody , but not by anti IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
During the challenge period , mice were treated with monoclonal antibodies directed against IL 4 , IL 5 , IFN gamma , or TNF alpha . ^^^ Treatment with antibodies to IL 4 , IFN gamma , or TNF alpha had no effect on eosinophilia . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokines assayed were EGF , interleukin 1 beta ( IL 1 beta ) , IL 2 , IL 3 , interferon alpha ( IFN alpha ) , IFN gamma , tumour necrosis factor alpha ( TNF alpha ) and transforming growth factor beta 1 ( TGF beta 1 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of E selectin on glomerular endothelial cells ( ECs ) was induced by interleukin 1 ( IL 1 ) , tumour necrosis factor ( TNF ) , interferon gamma ( IFN gamma ) and granulocyte / macrophage colony stimulating factor ( GM CSF ) ; this induction was inhibited by transforming growth factor beta ( TGF beta ) and by IL 4 . ^^^ Glomerular endothelial ICAM 1 expression was increased by IL 1 , TNF , IFN gamma , and GM CSF , while TGF beta inhibited induction by TNF and IL 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Also , mice fed on dietary 17 alpha methyl testosterone responded to heat killed Salmonella typhimurium with a higher increase in serum TNF , whereas the induced increase in the production of IL 10 by spleen cells was largely suppressed and no effect was found with respect to the production of IFN gamma and IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Proinflammatory cytokines such as tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and interleukin 1 beta are known modulators of bone remodeling in vitro and in vivo . ^^^ In cocultures of RAW 264 . 7 mouse macrophages stimulated with lipopolysaccharide and IFN gamma , and untreated MC3T3 E 1 osteoblasts , addition of anti TNF alpha antibody and inhibition of NO synthesis have additive , protective effects on osteoblast viability . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RANTES was only weakly induced ; however , concomitant treatment with TNF alpha and interferon gamma ( IFN gamma ) led to upregulation of RANTES , indicating a synergy between these two cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Alterations in IL 6 , IL 8 , GM CSF , TNF alpha , and IFN gamma release by peripheral mononuclear cells in patients with active vitiligo . ^^^ The results revealed ( 1 ) a significant increase in spontaneous production of IL 6 and IL 8 in patients ; ( 2 ) PHA , purified IgG from patients ( IgG anti MC ) , or IgG from normal controls ( N IgG ) induced a significant increase in IL 6 but diminished GM CSF , TNF alpha , and IFN gamma release in patients ; and ( 3 ) IgG anti MC brought about a significantly higher stimulatory effect on IL 1beta and IFN gamma production than N IgG in normal controls . ^^^ A significant decrease in TNF alpha and IFN gamma production may partially explain the reduced inflammatory reaction in vitiliginous lesions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Male CD 1 mice were used to test the in vivo effects of aflatoxin B 1 ( AFB 1 ) on the genetic expression of major cytokines produced by macrophages ( interleukin ( IL ) 1 alpha , IL 6 and tumour necrosis factor alpha ( TNF ) ) and splenic lymphocytes ( IL 2 , interferon gamma ( IFN gamma ) , and IL 3 ) , activated in vitro with lipopolysaccharide ( LPS ) and concanavalin A ( Con A ) , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Secreted Th 1 cytokines ( IFN gamma and TNF alpha ) stimulate macrophages and microglia to heightened phagocytic activity and the release of proinflammatory mediators . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These data suggest that increased production of IFN gamma and TNF alpha in tonsillar lymphocytes may play a key role in this kind of immunologic abnormalities . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A significant amount of IL 6 , IFN gamma , and TNF alpha was detected by enzyme linked immunosorbent assay in the culture supernatants of TL cultured with streptococcal antigen , even without any mitogens . ^^^ Furthermore , mRNA of IL 6 , IFN gamma , and TNF alpha was determined by reverse transcription polymerase chain reaction on the TL freshly isolated and with streptococcal stimulated antigen , whereas , production of IL 1 alpha , IL 4 , and IL 5 , and expression of their mRNA were not found on TL with or without streptococcal stimulation . ^^^ These data indicate that tonsils are highly active immunological organs containing a wide variety of cytokine producing cells , and suggest that IFN gamma , TNF alpha , and IL 6 may play an important role on the immune response to bacteria in the tonsil . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate the expression patterns of interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) , both of which are potent inducers of class 2 major histocompatibility complex ( MHC ) and intercellular adhesion molecule 1 ( ICAM 1 ) expression , and their codistribution with HLA DR and ICAM 1 in skin lesions , cultured fibroblasts , and peripheral blood mononuclear cells ( PBMC ) of systemic sclerosis ( SSc ) patients in different stages of disease . ^^^ RESULTS : In the early inflammatory stage of SSc , class 2 MHC and ICAM 1 expression could be detected on most endothelial cells and on fibroblasts located especially in perivascular areas surrounded by infiltrating lymphocytes , which belong to the T helper 1 phenotype expressing IFN gamma and TNF alpha . ^^^ CONCLUSION : These data suggest that class 2 MHC antigens and ICAM 1 on fibroblasts and endothelial cells are induced by IFN gamma and TNF alpha in an early stage of SSc after the influx of mononuclear cells , and may be important in the putative autoimmune response and in the perpetuation of fibrotic processes in SSc . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Semiquantitative RT PCR of isolated glomeruli at eight weeks demonstrated expression of cytokine mRNA for Th 1 CD4+ cells ( IFN gamma and TNF beta / LT , but not IL 2 ) , cytotoxic CD8+ T cells ( granzyme A and perforin ) , and macrophages ( TNF alpha and IL 10 ) , but not Th 2 CD4+ cells ( no increase in IL 4 , IL 5 and IL 6 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Short term cultured hepatocytes ( targets ) from HCV+ and HCV patients produced low levels of cytokines IL 1 beta , IL 2 , IL 6 , TNF alpha , and IFN gamma but a high level of IL 8 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , when HGF were stimulated with inflammatory cytokines such as IL 1 , TNF alpha and IFN gamma , the binding avidity between lymphocytes and HGF was significantly increased and the adhesion was mainly mediated by LFA 1 / ICAM 1 pathway . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Nasal epithelial cells from 32 patients ( HNE ) and bronchial epithelial cells from 17 Macaca nemestrina monkeys ( PBE ) were cultured in vitro for 24 to 72 h with LPS , TNF alpha , IL 1 beta , IFN gamma and TNF alpha combined with IFN gamma and / or dexamethasone at 10 to 1000 micrograms / ml . ^^^ Release of RANTES was stimulated by all activators except LPS in a time dependent manner , with the greatest synthesis induced by the combined addition of TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Research on gamma delta T cells in malaria infections suggests that gamma delta T cells are mediators of protective immunity , most probably through the production of Th 1 cytokines such as TNF alpha , TNF delta and IFN gamma and that excessive production of such cytokines may contribute to pathology . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study , we have investigated the modulatory role of PGE 2 , iloprost ( prostacyclin analogue ) , U 46619 ( thromboxane analogue ) on the release of IL 2 , IFN gamma , TNF alpha and IL 6 by T lymphocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PURPOSE : Recombinant viruses carrying DNA sequences encoding interleukin 2 ( ALVAC IL 2 ) , interferon gamma ( ALVAC IFN gamma ) , tumor necrosis factor alpha ( ALVAC TNF alpha ) , or the co stimulatory molecule B 7 1 ( ALVAC B 7 1 ) were investigated as agents for the treatment of a newly defined mouse prostate tumor model . ^^^ RM 1 tumor growth in C57BL / 6 mice was not affected by tumor cell expression of IL 2 alone , IFN gamma alone , or B 7 1 alone ; however , expression of TNF alpha alone significantly delayed tumor growth at early time points ( compared with parental ALVAC infected tumors , P = . 0001 at day 21 and P = . 037 at day 28 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OBJECTIVES : To determine circulatory levels of IL 1 beta , TNF alpha , IFN gamma and Neopterin in immunologically mediated recurrent abortion . ^^^ DESIGN : Blood samples were withdrawn from both groups of first trimester recurrent abortion and controls for the determination of serum IL 1 beta , TNF alpha , IFN gamma by the corresponding IRMA and serum neopterin by a double antibody RIA . ^^^ RESULTS : Serum IL 1 beta , TNF alpha and IFN gamma levels of the abortion group were significantly elevated when compared to the corresponding levels of controls . ^^^ CONCLUSIONS : In view of the proven findings that cytokines do have detrimental effects on implantation , trophoblast proliferation , embryo development and fetal survival , our results suggest a potential mechanism of immunologic recurrent abortion which involves the secretion of IL 1 beta , TNF alpha , IFN gamma and other cytokines by activated endometrial lymphocytes and macrophages in response to either trophoblast or sperm antigens and these cytokines could partially defuse or penetrate into the systemic circulation . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1 alpha , IL 2 , IL 10 , TNF alpha , and IFN gamma were not found . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Virus encoded IL 7 dramatically increased splenic cellularity in infected mice and enhanced the proliferative activity of T cells and their capacity to secrete IL 2 and IL 6 , but not IFN gamma , TNF alpha or IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin 12 treatment resulted in : 1 ) increased survival frequency ; 2 ) faster recovery from weight loss ; 3 ) substantially decreased VSV titers in brain homogenates and diminished immunohistochemical detection of VSV antigens in tissue sections ; 4 ) earlier and increased CNS expression of types 1 , 2 , and 3 nitric oxide synthase ( NOS ) and both major histocompatibility complex ( MHC ) class 1 and class 2 antigens ; 5 ) earlier and increased blood and CNS levels of tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
HIV 1 was isolated predominantly from CD4+ T cells with a CD45RO+ , CD25+ phenotype and was associated with a trend to elevated levels in culture supernatants of IFN gamma , IL 6 , TNF alpha , and IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although it could be shown that exogenously added TNF alpha acted synergistically with IFN gamma to induce NO release from the cell lines , an autocrine role for TNF alpha during HKS IFN gamma induction of NO synthesis could not be substantiated . ^^^ Neutralization of TNF alpha with a specific antibody completely blocked NO induction by exogenous TNF alpha but did not abrogate NO release either by HKS IFN gamma induced cells or by macrophages treated with supernatant from an HKS IFN gamma activated cell line . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
BTp 20 and LPS independently stimulated significant TNF alpha production by IFN gamma primed macrophages , the effect of BTp 20 + LPS ( 1 and 10 ng / ml ) combination was additive . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here we study the effects of IL 2 , IL 4 , IL 7 , IL 1beta , IL 12 , IFN gamma , TNF alpha , and granulocyte macrophage CSF ( GM CSF ) incorporated with peptide in adjuvant on a variety of responses elicited : CTL , T cell proliferation , cytokine production and message , and Ab isotype . ^^^ TNF alpha also synergized with IL 12 , but by a different mechanism , inducing IFN gamma production in BALB / c mice and thus shifting the response to a Th 1 phenotype . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 12 p 40 , IFN gamma , TNF , IL 1alpha , and IL 6 were shown to be increased , but IL 1beta was undetectable , in serum of MCMV infected mice . ^^^ The IL 12 p 40 , IFN gamma , TNF , and IL 6 responses were dramatic with peak levels reaching > 150 10 , 000 pg / ml at 32 40 h after infection and rapidly declining thereafter . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This late developing IFN gamma independent antileishmanial mechanism appeared to be dependent largely on endogenous tumor necrosis factor alpha ( TNF alpha ) : L . donovani infection induced TNF alpha mRNA expression in parasitized GKO livers and neutralization of TNF alpha reversed control at week 12 . 7 d of treatment of infected GKO mice with interleukin 12 ( IL 12 ) readily induced leishmanicidal activity and also partially restored the near absent tissue granulomatous response , observations that for the first time expand the antimicrobial repertoire of IL 12 to include IFN gamma independent effects . ^^^ These results point to the presence of an IFN gamma independent antimicrobial mechanism , mediated by TNF alpha , which remains quiescent until activated late in the course of experimental visceral leishmaniasis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Since adhesion molecules ( AM ) induced on endothelial cells ( EC ) play an important role in T cell migration into the CNS , the objective of this study was to examine the effect of IFN beta 1b on the expression of the AM , ICAM 1 , 5 CAM and E selection induced on EC by IFN gamma , TNF alpha , or IL 1 beta . ^^^ IFN beta 1b ( 1 1000IU / ml ) had minimal effect on basal expression of AM on HUVEC , but AM could be substantially upregulated by IFN gamma , IL 1 beta or TNF alpha . ^^^ The effect of IFN beta 1b on AM expression induced by IFN gamma , IL 1 beta or TNF alpha was slightly additive . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A rise in immunoreactive and bioactive TNF alpha and IFN gamma in draining lymph nodes , serum and foetal placental tissue is observed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Consequently , it was found that 24 h after the challenge IFN gamma , IL 6 and IL 1 beta were expressed and 48 h after the challenge IL 2 , IL 4 and TNF alpha were expressed in addition to these cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We also found IL 6 , TNF , and IFN gamma at lower levels in brain tissue from a patient with progressive multifocal leukoencephalopathy . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
On admission we found elevated plasma concentrations of TNF , IFN gamma , and IL 10 compared to levels during and after chemotherapy . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This includes norepinephrine synthesizing enzymes ( dopamine beta hydroxylase ( DBH ) and tyrosine hydroxylase ( TH ) ) , neuropeptide Y ( NPY ) , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , c fos protein , inducible nitric oxide synthase ( iNOS ) , and the macrophage cell marker ED 1 . ^^^ For immunodetection of TNF alpha , iNOS , IFN gamma and c fos , animals were injected 4 with saline or 100 microg of lipopolysaccharide ( LPS ) and were sacrificed at various time intervals post injection . ^^^ TNF alpha , IFN gamma , c fos , and iNOS proteins were observed in the spleen in a time dependent manner after LPS stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Associated with the lesions in the lungs were elevated levels of the pro inflammatory cytokines interleukin 6 ( IL 6 ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) that peaked at days 1 2 post instillation but resolved to normal limits by day 14 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
AM were treated with lipopolysaccharide ( LPS ) , interferon gamma ( IFN gamma ) , or tumor necrosis factor alpha ( TNF alpha ) in vitro , and nitrite , the stable oxidation product of NO , was assayed by the Griess reaction . ^^^ Rat AM produced NO in a dose and time dependent manner upon stimulation with LPS and / or IFN gamma , but not with TNF alpha . ^^^ The results presented here indicate that hamster AM , in contrast to rat AM , lack the ability to express iNOS protein and to generate NO in response to LPS , IFN gamma , or TNF alpha in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When all interferon ( IFN ) gamma , tumor necrosis factor ( TNF ) alpha and interleukin ( IL ) 1 alpha , or the combination of IFN gamma with either TNF alpha or IL 1 alpha were neutralized by antibodies , there was a significant reduction of iNOS expression and nitrite production in culture . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It has been demonstrated that endogenous cytokines including gamma interferon ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) , and interleukin 6 ( IL 6 ) play a protective role but that IL 4 plays a detrimental role in systemic Listeria monocytogenes infection in mice . ^^^ The expression of cytokine mRNAs including IFN gamma , TNF alpha , IL 4 , IL 6 , or IL 10 , which were amplified by reverse transcription PCR , was observed in intestinal intraepithelial lymphocytes irrespective of L . monocytogenes infection . ^^^ Increased numbers of L . monocytogenes were detected in the ileal contents of infected mice which received monoclonal antibodies ( mAbs ) against IFN gamma , TNF alpha , IL 4 , IL 6 , or IL 10 . ^^^ By contrast , mAbs against IL 4 or IL 6 showed little effect on the growth of L . monocytogenes in the mesenteric lymph nodes ( MLNs ) , spleen , and liver , but anti IFN gamma mAb and anti TNF alpha mAb suppressed the defense in these organs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We previously showed that injection of homogenous staphylococcal protein A 5 antigen fusion peptide into mice delayed allograft rejection and suppressed the major proinflammatory cytokines tumor necrosis factor alpha ( TNF alpha ) and gamma interferon ( IFN gamma ) associated with generation of protective granulomas . ^^^ Concomitant injection of Vh and an otherwise lethal dose of LPS ( 200 microg ) dramatically decreased levels of TNF alpha and IFN gamma in the spleen and peritoneal lavage fluid as compared to values determined for LPS alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In particular , we examined the expression of mRNA for helper T cell 1 ( Th 1 ) cytokines ( IL 2 , lymphotoxin , and gamma interferon [ IFN gamma ] ) , Th 2 cytokines ( IL 4 , 6 , and 10 ) , macrophage derived cytokines ( tumor necrosis factor alpha [ TNF alpha ] , IL 1beta , transforming growth factor beta [ TGF beta , IL 12p40 , and IFN gamma inducing factor [ IGIF ] ) , and inducible nitric oxide synthase ( iNOS ) in the lungs on days 1 , 3 , 7 , and 14 after infection and following treatment with IL 12 . ^^^ Similar results were also obtained for IFN gamma , IL 4 , IL 10 , and TNF alpha measured in the lung homogenates by enzyme linked immunosorbent assay . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The addition of TNF alpha to IFN gamma elevated class 2 expression and NO in both cells . ^^^ These results indicate microglia are more effective than astrocytes in producing cell associated and secreted immune mediators in response to IFN gamma and or TNF alpha and multiple parallel , but distinct , signaling events are required for cytokine induced class 2 MHC or NO production . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 10 / mice had a lower parasite burden and higher levels of serum TNF alpha , IL 12 , and IFN gamma compared with infected IL 10 + / + mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Only anti human IL 13 Abs inhibited IL 13 induced foreign body giant cell formation ; the fusion inducing effects of IL 13 continued to be observed in the presence of neutralizing Abs to IL 4 and several other anti cytokine Abs , including Abs against IFN gamma , granulocyte macrophage CSF , IL 3 , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It has been shown that neutrophils and macrophages play an important role to restrict bacterial replication in the early phase of primary infection in mice , and that the cytokines interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) are essential for protection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Transcripts for IL 1 alpha , IL 2 , IL 4 , IL 5 , IL 7 , TNF alpha , or IFN gamma were not detected in any of the meningioma cultures . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine levels ( IL 2 , 4 , 6 , TNF alpha , IFN gamma ) in AF were measured by an ELISA method . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Optimal control of IFN gamma and TNF alpha by IL 10 produced in response to one HLA DR mismatch during the primary mixed lymphocyte reaction . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Previous studies have reported that the levels of pro inflammatory cytokines , such as TNF alpha and IFN gamma , are elevated in the serum as well as in the cerebrospinal fluid of HAM / TSP patients . ^^^ The amount of cytokines in the supernatants of the cultured cells was determined by ELISA for TNF alpha , IFN gamma and TGF beta . ^^^ The suppression of IFN gamma varied and was weaker in some HAM / TSP patients compared to that of TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The authors describe the design and validation of a competitive RT PCR method for the efficient and reproducible quantitation of mRNA molecules of IFN gamma , TNF alpha , IL 4 and IL 10 in mouse spleen RNA extracts . ^^^ Normal mouse spleen tissue was found to contain 10 ( 7 ) 10 ( 8 ) molecules of TNF alpha , IFN gamma , IL 4 and IL 10 mRNA per micrograms total RNA extracted . ^^^ Injection of animals with anti CD 3 antibody , a well known cytokine inducer , resulted in a moderate increase in TNF alpha and IL 10 mRNA levels ( 14 and 24 fold , respectively ) , and in a substantially greater increase in the levels of mRNA for IL 4 and IFN gamma ( 199 and 851 fold , respectively ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The delta t gamma t variant specifically killed target cells , expressed the IFN gamma gene in response to Ag , and produced TNF alpha in response to anti CD 3 mAb , but it was affected in CD 3 ligand induced TCR / CD3 down modulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Production of IL 1beta , TNF alpha , IL 2 , and IFN gamma was determined after stimulation of whole blood samples with LPS and PHA , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
HIV 1 env DNA vaccine administered to rhesus monkeys elicits MHC class 2 restricted CD4+ T helper cells that secrete IFN gamma and TNF alpha . ^^^ All of the CD4+ T cell lines responded to Env peptide by secreting IFN gamma and TNF alpha without appreciable IL 4 production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunohistochemistry studies show that IL 10 / Fc treatment inhibits expression of TNF alpha , proinflammatory cytokine , as well as Th 1 type cytokines , IL 2 and IFN gamma , but promotes expression of IL 4 and IL 10 , Th 2 type cytokines , by islet infiltrating leukocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHOD : To evaluate the influence of gestation on the maternal immune system , we have measured , in pregnant women , the mRNAs coding for several cytokines ( TNF alpha , IFN gamma , GM CSF , IL 1 beta , IL 2 , IL 4 , IL 6 , and IL 10 ) in peripheral blood mononuclear cells , by use of semi quantitative RT PCR . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
First we demonstrated by immunofluorescence and immunoprecipitation that CD 43 is dys sialylated in monocytic THP 1 cells stimulated by interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) and stimulation increased correlated to heterotypic adhesion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Paired samples of milk and serum collected 3 days postpartum from 20 women were tested for the presence and level of interleukin ( IL ) 1 , IL 6 , IL 12 , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) by enzyme immunoassay . ^^^ Milk macrophages from eight subjects all exhibited mRNA for IL 1beta , TNF alpha , and IL 6 , and IFN gamma mRNA in six of eight subjects , although no IFN gamma was detected in any of the 20 samples of milk tested . ^^^ These observations suggest that the milk macrophages are actively engaged in the physiological production of IL 1beta , IL 6 , TNF alpha , and IFN gamma in the mammary gland and continue to possess the capacity to increase production of these cytokines in response to RSV and possibly other viral infections . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
High levels of several cytokines , including IFN gamma and TNF alpha , have been demonstrated in the salivary gland microenvironment of patients with Sjogren ' s syndrome ( SS ) . ^^^ We hypothesized that IFN gamma with or without ( + / ) TNF alpha could be playing a role in the pathogenesis of SS via the regulation of matrix metalloproteinase ( MMP ) levels . ^^^ This study examined the role of IFN gamma and ( + ) TNF alpha in the regulation of the matrix metalloproteinases , MMP 2 ( 72 kD gelatinase A ) and MMP 9 ( 92 kD gelatinase B ) . ^^^ A human salivary gland cell line ( HSG ) has been used as a possible in vitro model to study the role of IFN gamma + TNF alpha in the pathogenesis of SS . ^^^ The increase in MMP activity was partially blocked with an antibody against the IFN gamma receptor , and this was associated with a complete inhibition of the previously described IFN gamma + / TNF alpha antiproliferative effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Eosinophil adhesion to epithelial cells was increased when the BEAS 2B cells were pretreated with interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The induced serum levels of tumor necrosis factor , interleukin 6 , and interferon gamma ( IFN gamma ) were elevated and remained high for a prolonged period . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We determined whether endogenous interferon ( IFN ) gamma could be responsible for the observed increases in IL 1 and TNF alpha , because IFN gamma is a potent microglia / macrophage activator , and because its exogenous administration to rodents enhanced astrogliosis after adult or neonatal insults . ^^^ Third , in IFN gamma knockout adult mice , astrogliosis and increases in levels of IL 1alpha and TNF alpha were induced rapidly by injury . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Other proinflammatory cytokines such as interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) did not modify the spontaneous adhesion capacity of amebas . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Filter grown monolayers of the intestinal cell line HT 29 19A were incubated for 48 hours in basal medium containing 10 ng / ml TNF alpha and 5 U / ml interferon gamma ( IFN gamma ) . ^^^ RESULTS : After incubation with TNF alpha and IFN gamma , intestinal function altered , as shown by the increases compared with control values for G ( 22 . 8 ( 3 . 7 ) 5 ( 9 . 6 ( 0 . 5 ) mS / cm2 ) , Jman ( 33 . 8 ( 7 . 5 ) 5 7 . 56 ( 0 . 67 ) micrograms / h 10 cm 2 ) , and intact horseradish peroxidase ( 1 . 95 ( 1 . 12 ) 5 0 . 14 ( 0 . 04 ) micrograms / h 10 cm 2 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumour necrosis factor ( TNF ) , interleukin 2 ( IL 2 ) , interferon gamma ( IFN gamma ) and IL 5 were elevated in DTH reactive sponges compared with control sponges , and each cytokine had a relatively unique temporal profile . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) , soluble IL 2 receptor ( sIL 2R ) and monokines such as IL 1 beta , IL 6 , IFN alpha and tumour necrosis factor ( TNF alpha ) were not affected by IVIG supplementation to MLR . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both constitutive and interferon gamma ( IFN gamma ) , interleukin 4 ( IL 4 ) , IL 6 and tumour necrosis factor alpha ( TNF alpha ) induced human leucocyte antigen ( HLA ) DR , HLA DP and HLA DQ Ag expression on monocytes was significantly down regulated by calcitriol , IL 10 and transforming growth factor beta ( TGF beta ) . ^^^ The basal as well as IL 4 , IL 6 , IFN gamma , TNF alpha and TGF beta driven levels of HLA ABC Ag were significantly diminished by calcitriol . ^^^ Both the basal level of CD 71 and IL 4 , IFN gamma or TNF alpha driven expression was diminished on calcitriol treated U 937 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunoneutralization of the effects of cytokines was accomplished by administration of monoclonal antibodies against mouse IL 1 type 1 receptor ( IL 1R ) , TNF alpha , IL 6 or interferon ( IFN gamma ) prior to LPS . ^^^ The LPS induced release of cytokines was affected by previous immunoneutralization as compared with control experiments with normal immunoglobulin ( IgG ) : anti IL 1R did not affect serum TNF alpha but decreased serum IL 6 , anti TNF alpha decreased serum TNF alpha but not IL 6 , anti IL 6 did not affect serum TNF alpha but hugely increased IL 6 and anti IFN gamma decreased both serum TNF alpha and IL 6 . ^^^ Specific immunoneutralization of IL 1 , TNF alpha or IFN gamma did not prevent the LPS induced decrease in serum T 3 , T 4 and liver 5 ' deiodinase mRNA . ^^^ In conclusion , immunoneutralization of IL 1 , TNF alpha or IFN gamma did not prevent the LPS induced sick euthyroid syndrome in mice ; immunoneutralization of IL 6 , however , transiently inhibits the LPS induced decrease of liver 5 ' deiodinase mRNA . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pretreatment with CsA significantly reduced serum alanine aminotransferase ( ALT ) , serum tumor necrosis factor alpha ( TNF alpha ) production , without changing the TNF alpha mRNA level in the liver , and plasma interferon gamma ( IFN gamma ) , following LPS injection in this model . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , a mixture of cytokines ( interleukin 1 beta ( IL 1 beta ) , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) each at 10 ng ml 1 ) induced COX 2 mRNA expression , which was maximal at 12 h and inhibited by dexamethasone ( 1 microM ; added 30 min before the cytokines ) . ^^^ Incubation of the cells with the cytokine mixture ( IL 1 beta , TNF alpha , IFN gamma each at 10 ng ml 1 for 24 h ) caused the accumulation of PGE 2 and 6 keto PGF 1 alpha . 5 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , using reverse transcriptase polymerase chain reaction , expression of interleukin ( IL ) 2 , IL 12 , interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) and granulocyte / macrophage colony stimulating factor ( GM CSF ) but not IL 4 , IL 6 or IL 10 , was observed , a profile of cytokine expression which resembles that of a Th 1 immune response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , we observed the presence of IFN ( a mixture of IFN alpha , IFN beta , and IFN gamma , range 32 500 U / ml ) , TNF alpha ( range 32 100 U / ml ) , and IL 6 ( range 32 500 U / ml ) in all patients who had liver or lung cysts or both and displayed immunoreactivity against parasitic antigen ( antigen 5 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma increased transcript levels in all three types of glia , but TNF alpha increased levels only in oligodendrocytes Microglia constitutively transcribed three distinct TNFR 2 mRNAs , levels of which were increased by either IFN gamma or TNF alpha . ^^^ In contrast , astrocytes and oligodendrocytes constitutively transcribed nearly undetectable levels of TNFR 2 mRNAs , and levels were not affected by IFN gamma , TNF alpha , or oligodendrocyte maturation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cellular mechanisms , including macrophage and T cell infiltration , and cytokines like IFN gamma and TNF alpha are intimately involved in the pathogenesis of EAN . ^^^ P 2 protein reactive IFN gamma , TNF alpha , IL 1 beta , and IL 6 mRNA expressing lymph node cells were also downregulated in IL 10 treated compared to control EAN rats at Day 14 and 26 pi , while P 2 reactive IL 4 mRNA expressing cells were upregulated throughout treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inflammatory cells positive for the cytokines IL 2 , IL 4 , IL 6 , TNF alpha , and IFN gamma and for IL 2R , as well as CD8+ and CD4+ T cells and B cells were quantified using an immunoperoxidase technique in 25 fresh myocardial fragments from patients presenting with chronic chagasic cardiomyopathy . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The Th 1 subset of CD4+ T cells secretes cytokines usually associated with inflammation , such as IFN gamma and TNF and induces cell mediated immune responses . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The IFN gamma response is itself regulated by interaction with responses to other cytokines including IFN alpha / beta , TNF alpha , and IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Normal mice developed a chronic , asymptomatic infection , with rapid induction of mRNAs for IFN gamma , IL 12 , and TNF alpha in spleen , liver , and lung . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inflammatory cytokines IFN gamma plus TNF alpha induce regulated expression of CD 80 ( B 7 1 ) but not CD 86 ( B 7 2 ) on murine fibroblasts . ^^^ Here , we report that CD 80 can be expressed constitutively by murine fibroblasts and up regulated after treatment with IFN gamma plus TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of mRNA for IL 1beta , TNF alpha , and IFN gamma , known to enhance ICAM 1 expression , and MIP 1alpha , MIP 1beta , and MCP 1 , known to activate leukocyte integrins or to act as chemokines , were also induced in the glomeruli at a mRNA level in a profile similar to that of ICAM 1 expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have demonstrated here that retinal Mller glial ( RMG ) cells obtained from RCS dystrophic rats and stimulated in vitro with lipopolysaccharide ( LPS ) and interferon gamma ( IFN gamma ) accumulated higher levels of tumor necrosis factor ( TNF ) and inducible nitric oxide synthase ( NOS 2 ) mRNA and released in culture supernatants significantly higher amounts of TNF and nitrite compared to cells derived from nondystrophic controls . ^^^ The TNF and NOS 2 mRNA expression and TNF and nitrite synthesis induced in RMG cells from both strains by LPS + IFN gamma was significantly prevented by including transforming growth factor beta ( TGF beta ) in the culture medium . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immediately after M . pneumoniae reinfection of control immune mice , mRNAs for TNF alpha , IFN gamma , IL 1 beta , IL 6 , IL 2 and IL 2 receptor were promptly detected in the lungs . ^^^ In animals depleted of CD4+ T cells , mRNA expression for IL 2 , IL 2 receptor and IFN gamma were completely abrogated and mRNA expression for TNF alpha , IL 1 beta and IL 6 were reduced by 10 to 100 fold . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma and TNF alpha , included into the type 1 cytokines , appeared significantly increased in the allergic with respect to the non allergic asthmatic patients ( p = 0 . 01 ) and ( p < 0 . 001 ) respectively , while IL 10 , which belongs to the type 2 cytokines , was significantly increased in the non allergic asthmatic ( p < 0 . 001 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : We performed indirect immunohistochemistry studies of muscle tissue sections with a panel of 16 different cytokine specific monoclonal antibodies , directed against interleukin 1alpha , ( IL 1alpha ) , IL 1beta , IL 1 receptor antagonist ( IL 1Ra ) , IL 2 , IL 3 , IL 4 , IL 6 , IL 8 , IL 10 , IL 13 , interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , transforming growth factor beta 1 ( TGF beta 1 ) , TGF beta 2 , and TGF beta 3 in 5 untreated patients each with PM , DM , and IBM and in 4 normal controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study was based on the human colonic epithelial cell line HT 29 Cl . 16E , maintained as polarized monolayers on filters mounted in culture chambers and treated with various proinflammatory cytokines ( interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) , IL 1 beta ) alone or in combination . 3 . ^^^ IL 1 production , restricted to IL 1 alpha , was induced by the combination of IFN gamma / TNF alpha . ^^^ When IL 1 beta was added to IFN gamma / TNF alpha , it led to an additional production of IL 1 alpha . ^^^ Both IFN gamma / TNF alpha and IFN gamma / TNF alpha / IL 1 beta induced inducible nitric oxide synthase ( iNOS ) expression as shown by quantitation of NO 2 / NO3 by use of the Griess reagent , quantitation of cells scoring positive with an anti iNOS antibody and detection of mRNAs coding for iNOS by RT PCR . ^^^ The use of NG monomethyl L arginine ( L NMMA ) , an inhibitor of NOS , led to the demonstration of two distinct signalling pathways in IL 1 production by HT 29 Cl . 16E cells , one dependent on NO ( L NMMA sensitive ) under treatment with IFN gamma / TNF alpha / IL 1 beta , and the other independent of NO ( L NMMA insensitive ) under treatment with IFN gamma / TNF alpha . 5 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
While IL 6 and TNF titres measured in PBMC supernatants were not modified by addition of IL 12 , interferon gamma ( IFN gamma ) titres were up regulated and IL 10 production down regulated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) increased ICAM 1 expression on both cell types , although TNF alpha had a greater effect on oral than skin keratinocytes ( P < 0 . 05 ) and IFN gamma was more effective than TNF alpha on both cell types ( P < 0 . 01 ) . ^^^ In combination , TNF alpha and IFN gamma synergistically increased ICAM 1 expression on skin keratinocytes only , although ICAM 1 mRNA was synergistically increased in both cell types . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This shock was blocked by the injection of monoclonal antibodies ( mAbs ) to CD 4 , TCR Vbeta 7 plus Vbeta 8 , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) , but not by injection to CD 8 or unrelated Vbeta . ^^^ These results indicate that YPM induced shock requires the presence of CD4+ T cells bearing TCR Vbeta 7 and Vbeta 8 , and that endogenous TNF alpha and IFN gamma mediate the lethal effects . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The production of IFN gamma by these IELs as well as the mRNA for IFN gamma , TGF alpha , TGF beta 1 , TNF alpha and TNF beta in these IELs , in conjunction with the effect of produced cytokines on the expression of class 2 MHC molecules by the in vitro cell line IEC 6 , were investigated . ^^^ IFN gamma and TNF alpha [ corrected ] specific mRNA were detectable in all freshly isolated gammadelta , alphabeta and WT IELs . ^^^ In addition to the IFN gamma and TNF alpha [ corrected ] mRNA , alphabeta and WT IELs that had been activated in culture plates coated with anti CD 3 mAb contained mRNA for TGF beta 1 and TNF beta proteins . ^^^ In the cultured gammadelta IELs , however , the signals for IFN gamma and TNF alpha [ corrected ] transcripts were weak , and mRNA for the latter two cytokines was almost undetectable . ^^^ These results indicate that alphabeta IELs are superior to gammadelta IELs in the ability to produce IFN gamma , TGF beta 1 , TNF alpha [ corrected ] and TNF beta through TCR crosslinking primary in vitro stimulation . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of cytokine ( IL 1beta , IL 6 , IL 12 , IFN gamma and TNF alpha ) mRNA on microglia was not influenced by treatment with TGF beta1 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of glial cells with TNF alpha and IFN gamma induced only IP 10 , indicating that the expression of chemokine genes other than IP 10 requires a combination of different cytokines or direct cell cell contact between T cells and glia . ^^^ Quantitative assays revealed that activated astrocytes , the dominant glia of the CNS , express higher levels of chemokine transcripts than transcripts of the major proinflammatory cytokines TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The accumulation of leucocytes in milk and in teat cistern tissues , and the presence of the cytokines interleukin 1 beta ( IL 1 beta ) , tumour necrosis factor alpha ( TNF alpha ) , interleukin 8 ( IL 8 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) and interferon gamma ( IFN gamma ) in milk was studied during inflammation in the lactating ovine udder induced by Staphylococcus aureus and Escherichia coli . ^^^ TNF alpha , IL 8 and IL 1 beta were detected in milk during the inflammation , and low levels of GM CSF and IFN gamma were also observed occasionally . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Thus , the cytokine profile observed in this experimental model of allergic inflammation is characterized by IL 4 and IL 5 dominance , with an apparently minor TNF alpha and GM CSF contribution and relatively low or undetectable levels of IFN gamma and IL 10 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NOS activity was stimulated by exposure to interferon gamma ( IFN gamma ) and could be further stimulated by tumour necrosis factor alpha ( TNF alpha ) and epidermal growth factor ( EGF ) although neither was affective alone . ^^^ Messenger RNA levels for the inducible NOS isoform were increased by IFN gamma and TNF alpha in a manner consistent with the elevation of NOS activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) and Fas ligand can mediate potent inhibitory signals in haemopoietic cells . ^^^ In cultured BM cells , expression of TNFR 1 was not influenced by IFN gamma , TNF alpha or apoptosis inducing anti Fas monoclonal antibody ( mAb ) . ^^^ CD34+ cell Fas R expression was increased by IFN gamma and TNF alpha . ^^^ Potentiation of anti Fas mAb mediated inhibition of haemopoietic colony formation by IFN gamma and TNF alpha was observed . ^^^ These results suggest that , in addition to interacting at the level of intracellular signalling pathways , IFN gamma , TNF alpha or Fas ligand may potentiate or antagonize their effects through modulation of cytokine receptor expression . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RelB binds transcriptionally active kappaB motifs in the TNF alpha promoter in normal cells , and in vitro studies with macrophages isolated from RelB deficient animals revealed impaired production of TNF alpha in response to LPS and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , during the early course of infection , mutant mice responded by an elevated inflammatory type 1 cytokine response , as determined by IL 12 , IFN gamma , TNF alpha , and IL 1alpha specific RNA expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Splenocyte supernatants treated with anti IFN gamma or anti TNF alpha Abs suppressed inducible NO generation by macrophages . ^^^ Moreover , i . v . administration of anti IFN gamma and anti TNF alpha along with mannose HSA MDP greatly reduced protection against L . donovani infection . ^^^ Neoglycoprotein conjugated MDP , therefore , activated mouse macrophages in vivo to kill L . donovani , and this may depend on the physiologic generation of NO induced by IFN gamma and TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After stimulation of the HNECs in the presence of eosinophils , the secretion of RANTES , induced by a combination of TNF alpha and IFN gamma , appeared to have decreased . ^^^ After pretreatment of the eosinophils with anti CD 18 mAb or coculture with HNECs in Transwell culture inserts , these cells did not inhibit the TNF alpha and IFN gamma induced RANTES production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Exposure of human umbilical vein EC ( HUVEC ) to tumor necrosis factor alpha ( TNF alpha ) or to TNF alpha in combination with interferon gamma ( IFN gamma ) , but not to interleukin 1beta ( IL 1 ) , increased attachment to RADS vWF by about twofold . ^^^ Pretreatment of HUVEC with the combination of TNF alpha + IFN gamma produced a dramatic decrease in VNR expression , confirming previous findings of Defilippi et al . ^^^ These results suggest that in the presence of the inflammatory cytokines TNF alpha + IFN gamma , the endothelial GP Ib complex is a major determinant of HUVEC adhesion to surface bound vWF . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The clinical characteristics associated with this disease result from overproduction of Th 1 cytokines including interferon gamma ( IFN gamma ) , interleukin 2 ( IL 2 ) , and tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both chemokines are readily expressed in stromal cells after stimulation with pro inflammatory interleukin 1alpha ( IL 1alpha ) , interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and endotoxin lipopolysaccharide ( LPS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There was no difference between patients and controls in the production of IFN gamma , tumor necrosis factor ( TNF ) alpha and soluble interleukin ( IL ) 2 receptor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cisplatin induced tumor necrosis factor alpha ( TNF alpha ) mRNA expression , and an anti TNF alpha neutralizing antibody inhibited the induction of iNOS expression by a combination of cisplatin and IFN gamma in NB 39 nu cells . ^^^ Thus , iNOS expression in NB 39 nu cells by a combination of cisplatin and IFN gamma involves in the TNF alpha mediated signal transduction mechanism . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the brain , the onset of transcription varied from 45 min ( IL 1beta , TNF alpha ) to 4 hr ( IFN gamma ) , and the peak of mRNA accumulation was observed at different times depending on the cytokine and the brain region studied . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
All these cell lines showed the same cytokine production profile , secreting IL 1 alpha , IL 1 beta , LD 78 ( MIP l alpha ) , TNF alpha , IFN gamma , and GM CSF , but not secreting IL 1 alpha , IL 1 beta , IL 1 receptor antagonist ( IL 1 Ra ) , IL 4 , IFN alpha , and G CSF irrespective of the stimulatory agents used . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
But C 2 ceramide did not alter the higher secretion of TNF alpha or IL 1 beta from monocytes treated with IFN gamma or LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Natural killer ( NK ) cells were shown to secrete differentially interleukins ( IL ) , IL 1 alpha , IL 1 beta , IL 2 , IL 8 , interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and leukaemia inhibitory factor ( LIF ) upon stimulation with optimal concentrations of chylomicrons ( CM ) , very low density lipoprotein ( VLDL ) , low density lipoprotein ( LDL ) , high density lipoprotein ( HDL ) or acetyl modified low density lipoprotein ( AcLDL ) . ^^^ CM stimulated IL 8 > TNF alpha > IL 1 alpha > IL 2 = IFN gamma , and decreased seventeen fold GM CSF secretion . ^^^ VLDL stimulated IL 8 secretion > IL 1 alpha = IL 2 > IFN gamma > TNF alpha and decreased fivefold GM CSF secretion . ^^^ LDL stimulated IL 8 secretion > IL 1 alpha > IL 2 = IFN gamma , it did not modify TNF alpha and inhibited five hundred fold GM CSF secretion . ^^^ HDL stimulated IL 2 secretion = IFN gamma > IL 8 , it decreased GM CSF secretion > IL 1 alpha > IL 1 beta > TNF alpha without affecting LIF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In terms of effector immunity , beige mice showed enhanced expression of IFN gamma and tumour necrosis factor alpha ( TNF alpha ) when compared with wild type C57BL / 6 mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The hypermetabolic state persisted during subsequent anorexia , whose onset coincided with elevated IL 2 , and at the end of the acute phase of cachexia , the dual anorexic and hypermetabolic states were associated with the cytokines examined : TNF alpha , IL 1 beta , IL 2 , IL 4 , IL 5 , IL 6 , IL 10 , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The considerably lowered in vitro levels of LPS induced TNF alpha in these situations were significantly enhanced by IFN gamma , but did not reach the extremely high levels of IFN gamma primed naive cells from healthy donors . ^^^ Moreover , IFN gamma applied to septic patients with low monocytic HLA DR expression restored the deficient HLA DR expression and in vitro LPS induced TNF alpha secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Production of C 3 increased after stimulation with TNF alpha or IL 1beta and that of Bf with TNF alpha , TNF beta and IFN gamma . ^^^ TNF alpha , together with IFN gamma had synergistic effects and the release of C 3 or Bf increased to about 40 and 26 fold higher than those in control cells after incubation with both cytokines . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Exposure of PAEC to the combination of IFN gamma , TNF alpha , and IL 1 beta did not alter iNOS activity in cytosolic and membrane fractions but significantly ( p < 0 . 01 ) reduced eNOS activity in the membrane fraction , but not in the cytosolic fraction , after a 24 h exposure . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma exhibited no synergistic action with effector molecules , such as tumor necrosis factor ( TNF ) alpha and glucocorticoids . ^^^ Pretreatment of mice with IFN gamma significantly augmented the serum TNF alpha level and the serum cortisol level in response to LPS . ^^^ Therefore , we suggest that IFN gamma might augment LPS induced thymocyte apoptosis through elevating serum TNF alpha and cortisol levels . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
ISMCs were analyzed for class 2 major histocompatibility complex ( MHC ) , intercellular adhesion molecule 1 ( ICAM 1 ) , and B 7 before and after exposure to interferon gamma ( IFN gamma ; 100 or 1000 U / mL ) in the presence or absence of IL 1beta ( 10 ng / mL ) or TNF alpha ( 5 ng / mL ) for 72 hours . ^^^ When added with 100 U / mL IFN gamma , TNF alpha enhanced MHC class 2 and ICAM 1 expression on ISMCs and T cell proliferation . ^^^ However , TNF alpha and 1000 U / mL IFN gamma significantly decreased MHC class 2 expression and T cell proliferation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1 beta , TNF alpha and interferon gamma ( IFN gamma ) are known to stimulate a number of cells to produce inflammatory mediators such as prostaglandins . ^^^ We studied the effects of IL 1 beta , TNF alpha and IFN gamma on the induction of two isoforms of cyclo oxygenase and its relation to prostaglandin E 2 ( PGE 2 ) release and COX activity ( reflected by PGE 2 synthesis from exogenous arachidonic acid ) in human cultured airway smooth muscle cells . 3 . ^^^ IL 1 beta , but not TNF alpha or IFN gamma , caused a time and concentration dependent enhancement in PGE 2 and other prostanoid ( 6 keto PGF 1 alpha , PGF 2 alpha , thromboxane B 2 ( TXB 2 ) and PGD 2 ) production , with PGE 2 and 6 keto PGF 1 alpha as the principal products . ^^^ COX 2 protein measured by Western blot analysis was not detectable in untreated cells , but was increased in a time and concentration dependent manner by IL 1 beta , but not TNF alpha or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1alpha , IL 1beta , IL 2 , IL 6 and TNF alpha production from patient PBMCs was lower than that of control PBMCs , whereas production of IL 4 , IL 10 and IFN gamma was higher than that of control PBMCs . ^^^ Both in peritoneal and in pleural effusions concentrations of IL 1alpha , IL 1beta and IL 4 were not different from those measured in autologous serum , whereas those of IL 6 , IL 10 , TNF alpha , IFN gamma and sIL 2R were significantly higher . ^^^ The amounts of IL 1alpha , IL 1beta , IL 2 , IL 6 , TNF alpha and sIL 2R were higher in patient than in control sera , whereas those of IL 4 , IL 10 and IFN gamma were in the same range in patient and in control sera . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro , synovial fibroblasts exposed to tumour necrosis factor alpha ( TNF alpha ) in combination with interferon gamma ( IFN gamma ) showed enhanced expression of VCAM 1 , in comparison with fibroblasts from skin and lung and , unlike skin and lung fibroblasts , also expressed DAF and CR 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
An in situ hybridization method for determining cytokine mRNA synthesis in regional lymph nodes , after administration of Rolipram ( at days 2 7 ) , demonstrated a strong inhibitory effect on tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) mRNA expression , whereas no effects were seen on IL 2 mRNA synthesis after in vivo challenge with native RCII emulsified in Freund ' s incomplete adjuvant . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Linomide strongly suppressed IFN gamma and lipopolysaccharide ( LPS ) induced IL 1 beta , TNF alpha and IL 6 mRNA expression in macrophages in vitro as demonstrated by in situ hybridisation . ^^^ Macrophages from Linomide treated EAN rats showed decreased IL 1 beta , TNF alpha and IL 6 mRNA expression in response to IFN gamma and LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Antigen specific proliferation and IFN gamma production of the line were enhanced by exogenous IL 4 , TGF beta , IL 2 , IL 6 , IL 7 , and IL 9 and were inhibited by IL 10 and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of DEP on the release of IL 1 and TNF alpha by lipopolysaccharide ( LPS ) or interferon gamma ( IFN gamma ) primed AM were also studied . ^^^ In contrast , stimulation of AM production of TNF alpha by IFN gamma was not affected by DEP exposure . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Of various cytokines tested , including recombinant human ( rh ) interleukin 6 ( IL 6 ) , rh tumor necrosis factor alpha ( TNF alpha ) , interleukin 2 ( IL 2 ) , granulocyte / macrophage colony stimulating factor ( GM CSF ) , interferon alpha ( IFN alpha ) and interferon gamma ( IFN gamma ) , only TNF was able to re induce expression of ICAM 1 on cells from MCF 7 , SK BR 3 and ZR 75 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neutralization of endogenous IL 10 by monoclonal antibodies ( mAbs ) increases the secretion of tumor necrosis factor alpha ( TNF alpha ) ( +111 % ) , interferon gamma ( IFN gamma ) ( +92 % ) , and IL 12 ( +730 % ) 8 hours after Con A injection , and increases the hepatotoxicity , assessed by serum alanine transaminase ( ALT ) ( +174 % ) measurement and by histology , 24 hours after induction of hepatitis . ^^^ Conversely , preadministration of recombinant IL 10 reduces the production of these proinflammatory cytokines ( 47 % , 80 % , and 47 % for TNF alpha , IL 12 , and IFN gamma , respectively ) , and decreases neutrophil infiltration and ALT serum concentration ( 74 % ) 8 hours after Con A challenge . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The impaired response of fetal liver can not be attributed to alterations in the sensitivity of this organ to LPS or to proinflammatory cytokines involved in iNOS expression ( tumor necrosis factor alpha [ TNF alpha ] , interferon gamma [ IFN gamma ] ) , because cultured fetal and adult hepatocytes similarly responded to these factors . ^^^ Measurement of TNF alpha and IFN gamma levels in maternal and fetal serum showed a restricted permeability of placenta to these cytokines , results that were in agreement with the absence of iNOS expression in fetal liver . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Its main action probably results from inhibition of T lymphocyte activation and interference with secretion of cytokines like IL 2 , IL 4 , IFN gamma and TNF alpha . ^^^ We exposed CD 4 + cytotoxic T lymphocytes specific for myelin basic protein , a putative target autoantigen in MS , to CsA in vitro , and determined the drug ' s effects on proliferation , expression of high affinity IL 2R , secretion of the proinflammatory cytokines IFN gamma and TNF alpha as well as on the secretion of the chemokines MIP 1 alpha and MIP 1 beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cell recruitment phase , between 6 and 12 weeks of age , is predominated by production of the monokines IL 1alpha , IL 6 , and TNF After stimulation IFN gamma and occasional IL 10 and GM CSF producing cells could also be observed . ^^^ During the effector phase , between 4 and 6 months , there is a characteristic Th 1 cytokine profile with lymphocytes producing IL 2 , IFN gamma and TNF , supposedly TNF beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The current study was designed to explore the relative contribution of Fas ligand ( FasL ) , perforin , IFN gamma , and TNF alpha induced death pathways in the pathogenesis of CTL induced liver disease . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
BEAS 2B cell cultures were stimulated with ( 1 ) combinations of tumor necrosis factor alpha ( TNF alpha ) ( 50 2 , 000 U / ml ) / interferon gamma ( IFN gamma ) ( 20 500 U / ml ) / lipopolysaccharide ( LPS ) ( 10 micrograms / ml ) or ( 2 ) histamine ( 10 ( 3 ) M 10 ( 5 ) M ) . ^^^ Cell cultures treated with TNF alpha / IFN gamma / LPS in combination expressed iNOS mRNA , and this was associated with increases in supernatant nitrite concentrations over 24 h ; however , this response diminished with successive passage of cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It demonstrates that the depressed IL 1 , TNF , and IFN gamma release associated with melanoma may be mitigated by treatment with OK 432 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 10 alone did not induce MCP 1 or IL 8 secretion or steady state mRNA expression , nor modulate IL 1 beta , TNF alpha or IFN gamma induced IL 8 or MCP 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Instead , IL 10 prevented IFN gamma induced binding activity at the NF kappaB site of the tumor necrosis factor alpha ( TNF alpha ) responsive NF kappaB / C EBP composite element in the ICAM 1 promoter . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Experiments designed to investigate the role of tumor necrosis factor alpha ( TNF alpha ) in NO 2 production by Bryo and IFN gamma activated macrophages revealed that ANA 1 macrophages expressed low levels of TNF alpha mRNA constitutively that were not augmented in the presence of IFN gamma . ^^^ However , Bryo alone augmented the TNF alpha mRNA expression , which was only slightly increased with the addition of IFN gamma . ^^^ A polyclonal antibody to TNF alpha was able to completely neutralize TNF alpha secreted in either medium or Bryo plus IFN gamma treated cultures . ^^^ Neutralizing concentrations of anti TNF alpha antibody suppressed the Bryo plus IFN gamma induced NO 2 production approximately by 50 % , suggesting that NO 2 produced by Bryo plus IFN gamma treated ANA 1 macrophages may involve both TNF alpha dependent and TNF alpha independent mechanisms . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The purpose of this study was to evaluate the effects of VT dose on the expression of mRNAs for a cytokine profile consisting of TNF alpha , IL 1beta , IL 6 , IL 12 , IFN gamma , IL 2 , IL 4 , IL 5 , IL 10 , IL 12 , and TGF beta and to measure the kinetics of these responses . ^^^ Both 5 and 25 mg / kg VT significantly induced the mRNAs for the proinflammatory cytokines IL 1beta , IL 6 , and TNF alpha ; the T helper 1 cytokines IFN gamma and IL 2 ; and the T helper 2 cytokines IL 4 and IL 10 , whereas lower doses had no effect . ^^^ The duration of elevated mRNA expression in spleen was 1 8 hr for TNF alpha , IL 6 , IFN gamma , and IL 10 and was 1 4 hr for IL 1beta , IL 12p40 , IL 2 , and IL 4 . ^^^ In Peyer ' s patches , duration periods were 1 8 hr for IL 6 , IL 2 , and IL 10 ; 1 4 hr for IL 1beta , IL 12p40 , and TNF alpha ; and 1 2 hr for IFN gamma . ^^^ Serum levels of TNF alpha , IL 6 , and IFN gamma were elevated 3 hr after exposure to 25 mg / kg VT , thus suggesting that elevation of splenic and Peyer ' s patch mRNA abundance correlated with increases in systemic production of these cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Antibody neutralization studies further revealed that the tumoricidal and differentiating effects of PSCS MNC CM were mainly derived from the elevated cytokines , especially IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Quantitative determinations of interleukin 1 beta ( IL 1 beta ) and interleukin 6 ( IL 6 ) in the culture supernatants were performed with a commercial ELISA ; the levels of tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) were measured by specific radioimmunometric assays . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Because cytokines often work in a cascade fashion and keratinocytes are a source of primary cytokines , IL 1 alpha , and tumor necrosis factor alpha ( TNF alpha ) , we examined whether combined treatment with IFN gamma and these primary cytokines , IL 1 alpha and TNF alpha , had a synergistic effect on the production of IL 6 and IL 8 . ^^^ Combined treatment with IFN gamma and TNF alpha induced 11 fold to 12 fold higher levels of IL 6 than TNF alpha alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A combination of tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) enhanced the expression of iNOS in murine cerebral endothelial cells ( MCECs ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Herein , we determine whether the TNF alpha stimulating region of Gal lectin can activate gamma interferon ( IFN gamma ) primed BMM for NO production and amebicidal activity . ^^^ Native Gal lectin ( 100 to 500 ng / ml ) stimulated TNF alpha and inducible nitric oxide synthase ( iNOS ) mRNA expression in IFN gamma primed BMM as did lipopolysaccharide ( 100 ng / ml ) . ^^^ These results suggest that the TNF alpha stimulating region of native Gal lectin is a potent stimulus of IFN gamma primed BMM for NO production , which is essential for host defense against amebiasis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Three corneas from each respective group were excised for quantitation of interleukin 1beta ( IL 1beta ) , tumor necrosis factor alpha , and gamma interferon ( IFN gamma ) by enzyme linked immunosorbent assay . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Levels of lung IFN gamma and TNF alpha were elevated in Igh / mice compared to those in controls . ^^^ Of most importance , however , congenitally IFN gamma deficient Ifg / mice ( which have elevated levels of other cytokines , including TNF alpha and granulocyte macrophage colony stimulating factor ) are paradoxically more resistant to MoPn rechallenge than controls , showing that IFN gamma is not an absolute requirement for acquired resistance and implying the presence of very effective compensatory host defense mechanism ( s ) . ^^^ Thus , resistance to reinfection in this model is flexible and multifactorial and is heavily dependent on CD4+ T cells , with a probable role for IFN gamma and TNF alpha and a possible modest role for Th 1 dependent antibody . ^^^ Since IFN gamma was dispensable in host defense , the highly effective mechanism or mechanisms which can compensate for its absence ( which include TNF alpha ) deserve further study . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It might be useful for differential diagnosis among patients with various autoimmune diseases such as SLE , SS , IBD , and MS to measure the serum levels of cytokines such as IL 10 , IFN gamma , and TNF alpha using ultrasensitive enzyme linked immunosorbent assay system . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study analyses the production of tumour necrosis factor ( TNF ) alpha and soluble TNF receptor ( sTNF R ) before and after exposure to gamma irradiation and interferon gamma ( IFN gamma ) in 12 cell lines derived from Ewing ' s sarcoma ( ES ) / peripheral primitive neuroectodermal tumours ( pPNET ) . ^^^ Priming of cultures with recombinant human IFN gamma ( rhIFN gamma ) markedly enhanced TNF alpha secretion in the radiation responsive cell lines and had no influence on sTNF Rp 55 and sTNF Rp 75 levels . rhIFN gamma affected the magnitude rather than the sensitivity of the radiation response . ^^^ Collectively our findings indicate that IFN gamma priming potentiates the secretion of bioactive TNF alpha by ES / pPNET cells in response to gamma irradiation without affecting sTNF R release . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Hyperproduction of TNF alpha was temporally correlated with virus induced production of interferon gamma ( IFN gamma ) ; only marginally increased IFN gamma and TNF alpha production was observed in LCMV infected , T cell deficient mice and in mice infected with vesicular stomatitis virus , a virus that induces much less T cell activation than does LCMV . ^^^ Finally , LCMV infection was much less efficient in priming IFN gamma knockout mice for hyperproduction of TNF alpha . ^^^ These findings indicate that clinically silent viral infections may induce hypersensitivity to LPS through T cell activation and subsequent production of IFN gamma ; this sensitizes monocytes / macrophages for hyperproduction of TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neonatal susceptibility to LPS and SEB correlated directly with plasma TNF alpha but not IFN gamma levels , which was confirmed by TNF alpha and IFN gamma blockade experiments . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokine mRNAs expressed in the foot pads and spleens of BALB / cAJcl mice infected with Mycobacterium leprae were studied by the reverse transcriptase polymerase chain reaction ( RT PCR ) method using cytokine specific primers for interleukin 1 alpha ( IL 1 alpha ) , 2 , 4 , 6 , 10 , 12 ( p 40 ) , gamma interferon ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and TNF beta , and then for CD 4 and CD 8 markers . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the same animals , anti P selectin mAb significantly inhibited neutrophil and monocyte migration to dermal inflammatory reactions induced by zymosan activated rat serum ( ZAS ) containing the chemotactic factor C5ades Arg , endotoxin ( LPS ) , interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cells were cultured for 24 h in the presence or absence of IFN gamma , TNF alpha , IL 1 beta , IL 4 , IL 6 , IL 8 , IL 10 , and TGF beta 1 . ^^^ IFN gamma at 500 U / ml and TNF alpha at 200 ng / ml upregulated ICAM 1 expression ; IL 1 beta at 100 pg / ml upregulated ICAM 1 on WK cells only . ^^^ Cells cultured in the presence of both IFN gamma and TNF alpha exhibited a mean fluorescence intensity far greater than those cultured with IFN gamma or TNF alpha alone . ^^^ IFN gamma and TNF alpha enhanced the release of sICAM 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The production of the cytokines tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) and interleukin 4 ( IL 4 ) , was analysed with all three methods . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This was associated with an increase in the number of interferon gamma ( IFN gamma ) , IL 1 and tumour necrosis factor alpha ( TNF alpha ) transcripts and a decrease in IL 4 and IL 10 transcripts . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After antigenic stimulation , naive CD8+ T cells differentiate into cytotoxic Tc 1 cells secreting the cytokines IL 2 , IFN gamma , and TNF , which aid their proliferation and effector functions . ^^^ We now show that these cells also have a defect in the synthesis of IFN gamma , TNF , and IL 10 in response to antigenic stimulation . ^^^ IL 2 synthesis was the most sensitive , as stimulation of IL 4 treated Tc 1 cells with higher numbers of APCs partially restored IFN gamma , TNF , and IL 10 , but not IL 2 , synthesis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
BACKGROUND : Isolated limb perfusion ( ILP ) with tumor necrosis factor alpha ( TNF alpha ) + / interferon gamma ( IFN gamma ) and melphalan in patients with primarily irresectable soft tissue sarcoma is promising in terms of tumor regression and limb salvage . ^^^ METHODS : Fifteen patients with irresectable soft tissue tumors of the limb underwent ILP with TNF alpha , + / IFN gamma , and melphalan . ^^^ CONCLUSIONS : In patients with irresectable soft tissue tumors , multimodality treatment using ILP with TNF alpha + / IFN gamma and melphalan , tumor resection , and postoperative high dose radiotherapy is associated with a considerable risk of tissue necrosis and impaired healing . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Normal mouse epidermis expressed interleukin ( IL ) 1 alpha , interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) and IL 6 mRNAs . ^^^ Similarly , in a chronic model , essential fatty acid deficiency , epidermal levels of TNF alpha , IL 1 alpha , IL 1 beta and IL 6 mRNAs , but not IFN gamma mRNA , were elevated over controls ; and again , mRNAs for the remaining probed cytokines were not detected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 2 , IFN gamma , and TNF beta producing cells were undetectable in most patients with CMV pneumonitis ( n=7 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , the secretion of cytokines , i . e . , IFN gamma , IL 1beta , IL 2 and TNF alpha , by peripheral blood mononuclear cells ( PBMC ) was differently modulated by the tumor cell supernatants , e . g . , the production of IFN gamma was reduced in normal PBMC stimulated with PHA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1 through IL 6 , IL 8 , RANTES , TNF alpha , IFN gamma and GM CSF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The secretion of C 4 and factor B in ductal cell lines ( PANC 1 and MIA PaCa 2 ) was independently regulated by interleukin ( IL ) 1 beta , tumor necrosis factor ( TNF ) alpha , and interferon ( IFN ) gamma ; C 4 secretion was induced by IFN gamma , whereas factor B secretion was induced by IL 1 beta , TNF alpha , or IFN gamma . ^^^ These observations indicate that : ( a ) complement C 4 and factor B are secreted into the exocrine pancreas , ( b ) ductal epithelial cells appear to be the site of C 4 and factor B biosynthesis , and ( c ) local secretion of C 4 and factor B in the pancreas is differentially regulated by IL 1 beta , TNF alpha , and IFN gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
VEE induced cytokine gene expression was 100 fold elevated over that of untreated controls for IFN gamma and IL 6 and 10 fold increased for IL 12 , IL 10 , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1beta , IFN gamma , or TNF alpha caused highly significant dose and time dependent HUVEC injury . ^^^ Ab blockade of ICAM 1 on HUVEC inhibited 50 to 70 % of the injury induced by these cytokines , whereas a mAb to E selectin reduced 45 to 65 % of IL 1beta and TNF alpha , but not IFN gamma induced cytotoxicity . ^^^ Thus , in this in vitro model of vasculitis , the effect of IL 1beta , IFN gamma , and TNF alpha as promotors of cytokine mediated neutrophil dependent injury to HUVEC is a process dependent on expression of adhesion molecules and probably associated with NO produced in the system . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Adding low doses of 100 U / ml IFN gamma plus 10 U / ml TNF alpha to bulk cultures of dissociated HNSCC cancers diminished the frequency of CD34+ cells . ^^^ In HNSCC bulk cultures containing high levels of CD34+ NS activity , IFN gamma / TNF alpha not only reduced CD34+ cell levels , but also increased the capacity of the intratumoral T cells to express the p 55 IL 2R . ^^^ These studies show that IFN gamma / TNF alpha can induce differentiation of TGF beta secreting CD34+ NS cells into nonsuppressive monocytic cells that secrete TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The 233 bp MART 1 / Melan A promoter does not appear to have cytokine ( IL 2 , IL 4 , IL 7 , GM CSF , TNF alpha or IFN gamma ) responsive elements when studied in transient transfection assays . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) and IL 1beta produced a significant enhancement of VLA alpha 6 expression in vitro in thyrocytes in culture . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The production of IL 2 , IL 4 , IL 10 , IFN gamma and TNF alpha was determined in supernatants of ConA stimulated splenocytes and that of IL 1 in the supernatants of LPS stimulated peritoneal macrophages . ^^^ A gradual age related decline was observed in the production of IL 2 and IFN gamma , whereas the levels of IL 4 , IL 10 , IL 1 and TNF alpha progressively increased with aging , in unimmunized BALB / c and C3H . ^^^ The characteristic cytokine profile following immunization of 2 month old mice was early increased production of TNF alpha and IL 1 , followed by a peak of Th 1 type cytokines ( IL 2 , IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Supernatants from adriamycin stimulated cells were also analyzed at 6 , 8 , and 24 hr for TNF , IL 1 , and IFN gamma ( ELISA ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Clones derived from MS patients produced increased levels of IL 2 , IL 4 , tumor necrosis factor alpha ( TNF alpha ) , IFN gamma , and IL 10 , but not IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A semiquantitative analysis of the mRNA expression of some of these proteins by reverse transcriptase polymerase chain reaction showed that these cytokine effects were pretranslational as there was enhancement of factor B mRNA expression by IL 1 , TNF alpha , IFN gamma , IL 6 and endotoxin , but only IFN gamma enhanced C 1 inhibitor and C 4 mRNA expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
High numbers of interleukin 2 ( IL 2 ) mRNA expressing cells and also interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) expressing cells could be identified . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Analysis of cDNA from infected tissues using a competitive polymerase chain reaction excluded interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) , and interleukin 6 ( IL 6 ) as mediators of the enhanced resistance of the nude mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines , including IFN gamma , IL 1 alpha , IL 6 and TNF alpha have been shown to modulate expression of ICAM 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Addition of antiserum against bovine interferon gamma ( IFN gamma ) reproducibly neutralized the inhibitory effect of the T cell supernatants , whereas addition of antisera against bovine tumor necrosis factor alpha ( TNF alpha ) had no effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The controlled production of IL 10 , IFN gamma , and TNF alpha coupled with increased production of reactive oxygen metabolites during early phase of infection distinguished less susceptible C3H / HeN mice from their more susceptible cogenic C3H / HeJ counterparts . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In cultured microglia or astrocytes , LPS and to a much lesser degree IL 1beta , but not IFN gamma or TNF alpha , induced the expression of IL 12 p 40 mRNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Suppression of concanavalin A induced hepatitis in IFN gamma ( / ) mice , but not in TNF alpha ( / ) mice : role for IFN gamma in activating apoptosis of hepatocytes . ^^^ To elucidate the pathogenesis of this disease , we analyzed the roles of IFN gamma and TNF alpha using deficient mice of these cytokines . ^^^ Development of hepatitis was reduced significantly in IFN gamma ( / ) mice , while susceptibility of TNF alpha ( / ) mice was not changed . ^^^ Interestingly , apoptotic cell death was observed in the affected livers of control or TNF alpha ( / ) mice , but not in those of IFN gamma ( / ) mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study , we show that combined stimulation of RCS dystrophic retinal Mller glial ( RMG ) cells with interferon gamma ( IFN gamma ) and lipopolysaccharide ( LPS ) induced the release in culture supernatants of significantly higher amounts of tumor necrosis factor ( TNF ) and nitric oxide ( NO ) compared to nondystrophic congenic controls . ^^^ The addition of TGF beta with LPS + IFN gamma clearly inhibited TNF release in supernatants from both dystrophic and control rat RMG cells and microglia . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In order to improve suppressed immunogenicity of QRpP cells with the high levels of PGE 2 , the author transfected QRpP cells with an effective expression vector containing cDNA for IL 2 , IFN gamma and TNF alpha . ^^^ These transfected clones expressed mRNA for IL 2 , IFN gamma and TNF alpha , respectively , and produced high levels of cytokines in their culture supernatants . ^^^ Consequentry , QRpP cells transfected with IL 2 ( QRpP IL 2 ) , IFN gamma ( QRpP IFN gamma ) , IL 2 and IFN gamma ( QRpP IL 2 / IFN gamma ) , TNF alpha ( QRpP TNF alpha ) lowered in tumorigenicity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Induction of HSP 70 in human granulosa luteal cells was also seen with interferon ( IFN ) gamma ( 10 ng / ml ) , tumour necrosis factor ( TNF ) alpha ( 100 ng / ml ) and a combination of IFN gamma / TNF alpha ( 10 / 50 ng / ml ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Biopsies of the ovary were obtained at surgery and stored in liquid nitrogen until processed by reverse transcription polymerase chain reaction amplification to identify the presence of mRNA for interleukin ( IL ) 1 , IL 2 , IL 4 , IL 6 , IL 8 , IL 10 , IL 13 , tumour necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) . ^^^ There was no significant difference in the expression of IL 8 , IL 13 , IFN gamma and TNF alpha mRNA in the two groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neither in vitro hyperthermia at 41 degrees C for 3 or 6 hr nor cytokines such as gamma interferon ( IFN gamma ) or tumor necrosis factor alpha ( TNF alpha ) induced ICAM 1 expression in B 16 melanoma cell lines . ^^^ A combination of IFN gamma with TNF alpha caused slight induction of ICAM 1 expression in the B 16 F10 melanoma cell line . ^^^ In contrast , they showed a clear increase in the expression of ICAM 1 after treatment with either with IFN gamma or TNF alpha , and the expression was further augmented by a combination of the two cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Transcripts for IL 1 alpha , TNF alpha , and TGF beta 1 were present in most corneal samples ; whereas those for IFN gamma and CD 4 were not detected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We show here that treatment of RMG cells from both strains with LPS + IFN gamma does not induce TNF synthesis in culture supernatants but produces high amounts of NO only in the supernatants from activated C3H / HeN RMG cells . ^^^ The addition of TNF in the culture medium containing LPS / IFN gamma further increases nitrite production in C3H / HeN RMG cells and allows the synthesis of low levels of nitrite in C3H / HeJ RMG cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
High levels of cells expressing mRNA for gamma interferon ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) were detected 12 to 48 h postinoculation ( p . i . ) in splenocytes , and large numbers of IFN gamma secreting cells were present in the spleen on day 3 p . i . ^^^ To elucidate their regulatory importance , Aabs dose dependently inhibited IFN gamma production by splenocytes , IFN gamma induced major histocompatibility complex expression by peritoneal macrophages , and TNF alpha induced thymocyte proliferation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
SW strain showed a modest increase of TNF alpha , IFN gamma , and IL 4 producing cells after 4 6 days , and a very distinct increase of IL 4 producing cells on days 8 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Major histocompatibility complex molecule expression could be further increased by intradermal injection of pig interferon gamma ( IFN gamma ) but not human IFN gamma or tumor necrosis factor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : The activators examined were interleukin 1 alpha ( IL 1 alpha ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Exposure to 5 also affected the inducible production of interleukin 6 ( IL 6 ) and interferon gamma ( IFN gamma ) , but apparently not that of tumor necrosis factor alpha ( TNF alpha ) or IL 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have examined the bradykinin ( BK ) stimulated production of prostacyclin ( PGI 2 ) by cultured human pulmonary artery smooth muscle cells ( HPASMC ) pretreated with lipopolysaccharide ( LPS ) , interleukin 1 beta ( IL 1 beta ) , tumor necrosis factor alpha ( TNF alpha ) or interferon gamma ( IFN gamma ) . ^^^ A striking increase in PGI 2 production in response to BK was observed in HPASMC which had been incubated with 1 microgram / ml LPS , 20 U / ml IL 1 beta , 50 U / ml TNF alpha or 100 U / ml IFN gamma . ^^^ After incubation with various concentrations of LPS or cytokines , the production of PGI 2 by BK stimulated HPASMC showed significant , dose dependent increases beginning at 0 . 1 microgram / ml of LPS , 2 U / ml of IL 1 beta and 5 U / ml of TNF alpha , while higher concentrations of IFN gamma failed to cause any further increase in PGI 2 production . ^^^ LPS , IL 1 beta and TNF alpha effectively enhanced BK stimulated production of PGI 2 by HPASMC , while IFN gamma had only a weak effect on BK stimulated PGI 2 production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TBM was associated with elevated concentrations of TNF alpha , sTNFR 75 , sTNFR 55 , IFN gamma , and IL 10 , while CSF IL 12 was undetectable in all TBM patients . ^^^ The levels of TNF alpha did not decrease over time , being still detectable in the CSF 16 months after starting antibiotic therapy , whereas IFN gamma along with anti inflammatory mediators sTNFR 75 , sTNFR 55 , and IL 10 remained elevated in the CSF for 4 8 months . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of genes encoding cytokines ( TNF alpha / beta , IL 1 alpha / beta , IL 2 , IL 3 , IL 4 , IL 5 , IL 6 and IFN gamma ) , cytokine receptors ( TNF Rp 55 and p 75 , IL 1R p 60 and p 80 , IFN gamma R , and IL 6R ) , the cell adhesion molecule ( ICAM 1 ) , inducible nitric oxide synthetase ( iNOS ) , anti chymotrypsin ( EB22 / 5 . 3 ) , and the gliotic marker ( GFAP ) was evaluated over a 6 month period using a sensitive RNase protection assay ( RPA ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : In vitro production of interleukin ( IL ) 12 , IL 10 , tumor necrosis factor alpha , interferon gamma ( IFN gamma ) , and transforming growth factor beta ( TGF beta ) from purified monocytes and peripheral blood mononuclear cells , cytokine expression on duodenal biopsy specimens , and serum cytokine and immunoglobulin ( Ig ) levels were tested in 9 patients with WD . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In order to get a better insight into cytokine network regulation in systemic lupus erythematosus ( SLE ) , we analyzed levels of interleukin 10 ( IL 10 ) , interleukin 6 ( IL 6 ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) in the sera from 36 SLE patients . ^^^ TNF alpha and IFN gamma did not differ from normal values . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here we studied the potential of cytokines ( TNF alpha , IFN gamma , IL 1 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The results demonstrate that the majority of these CTL clones secrete IFN gamma , TNF alpha , macrophage inflammatory protein 1alpha and 1beta , IL 16 , and matrix metalloproteinase 9 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We now investigated iNOS mRNA expression and enzyme activity in primary cell cultures of rat microvascular brain endothelial cells after treatment with the proinflammatory cytokines interleukin 1beta ( IL 1beta ) , Tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) alone or in combination . ^^^ All combinations of two cytokines , including that of TNF alpha and IFN gamma or the triple combination led to expression of iNOS mRNA and active protein . ^^^ Cell activation by the combination of TNF alpha + IFN gamma led to an early expression of IL 1beta by the endothelial cells suggesting iNOS induction as a consequence of endogenous IL 1beta production under this challenge . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Antibody neutralization studies indicate that TGF beta 1 , IL 1 , TNF alpha , macrophage inflammatory protein ( MIP ) 1alpha , or IFN gamma are not responsible . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cultures of these microvascular and macrovascular endothelial cells were incubated with interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and Salmonella typhimurium lipopolysaccharide ( LPS ) alone or in combination , and nitrite production was measured . ^^^ Single agent exposure with IFN gamma ( up to 1 , 000 U / ml ) , TNF alpha ( up to 60 , 000 U / ml ) , or LPS ( up to 500 ng / ml ) had little effect on nitrite generation . ^^^ Nitrite production by rat aortic macrovascular endothelial cells ( RAEC ) was significantly greater than that by the rat lung microvascular endothelial cells ( RLMVEC ) when stimulated with TNF alpha + IFN gamma , LPS + IFN gamma , or TNF alpha + LPS . ^^^ The nitrite generation from rat pulmonary artery endothelial cells was intermediate between RAEC and RLMVEC responses when stimulated with IFN gamma + LPS or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have investigated the potent influence of some cytokines , tumour necrosis factor alpha ( TNF alpha ) , platelet derived growth factor ( PDGF ) , basic fibroblast growth factor ( bFGF ) and interferon gamma ( IFN gamma ) , on the secretion of cysteine proteinases ( cathepsins B and L ) by cultured synovial fibroblast like cells from patients with rheumatoid arthritis ( RA ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Second , the authors performed an adhesion assay with 51Cr labeled monocyte depleted PBL from normal , healthy volunteers and cultured HCE cells , with and without treatment of IFN gamma or TNF alpha in 96 well plates for 1 hour at 37 degrees C in 5 % CO 2 . ^^^ In addition , both IFN gamma and TNF alpha increased ICAM 1 expression on the cultured HCE cells dramatically . ^^^ Neither IFN gamma nor TNF alpha had any effect on LFA 3 expression on the cultured HCE cells . ^^^ The PBL adhesion to the HCE cells with and without treatment of IFN gamma or TNF alpha was blocked dominantly by anti ICAM 1 or anti LFA 1 alpha mAb . ^^^ The adhesion percentile of the PBL applied to the HCE cells pretreated with IFN gamma or TNF alpha showed a dose response curve dependent on the concentration of these cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Th 2 cytokines such as interleukin 4 ( IL 4 ) or interleukin 10 ( IL 10 ) are predominantly produced locally in the uterine and placental tissues , whereas the production of Th 1 cytokines such as tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) are decreased . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , interleukin ( IL ) 1beta , tumour necrosis factor alpha ( TNF alpha ) , IL 4 and interferon gamma ( IFN gamma ) concentrations were measured in supernatants of alveolar cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Anti CD 3 mAb as well as SEB or Con A induced the release of systemic tumor necrosis factor ( TNF ) , interferon gamma ( IFN gamma ) , and various other cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this report , we show that rat SchC in vitro express both C 3 mRNA and protein in response to dibutyryl cyclic AMP ( dbcAMP ) and the cytokines IFN gamma , TNF alpha , and IL 1beta . ^^^ This response was minimally enhanced by costimulation with 100 U / ml IFN gamma , whereas costimulation with 100 U / ml IFN gamma together with 150 450 ng / ml TNF alpha induced a greatly increased C 3 response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However when either LPS and IFN gamma or fixed S . aureus Cowan ( SAC ) are used to activate monocytes , IL 10 is a much more potent inhibitor of TNF alpha production than is IL 13 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma induced HLA DR but not ICAM 1 expression on cultured dermal papilla cells is downregulated by TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The capacity of stimulated peripheral blood cells from infected , intensive care patients to produce cytokines ( IFN gamma , tumor necrosis factor alpha ( TNF alpha ) and interleukin 6 ( IL 6 ) ) was compared to that of healthy donors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have previously shown that cross linking of CD 4 molecules ( CD4XL ) in normal peripheral blood mononuclear cells ( PBMC ) results in secretion of cytokines tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) , but not of interleukin 2 ( IL 2 ) or IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The addition of IFN gamma increased ovalbumin presentation even in the presence of TNF alpha or LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Previous studies on T lymphocyte bulk cultures and T lymphocyte lines with specificity for different antigens suggested that the drug might partially act via suppression of T cell proliferation and secretion of proinflammatory cytokines like interferon gamma ( IFN gamma ) and / or tumor necrosis factor alpha ( TNF alpha ) . ^^^ There was no inhibitory effect on the secretion of IFN gamma , TNF alpha , and macrophage inflammatory protein ( MIP ) 1 alpha , but release was rather slightly enhanced . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To investigate the role and the activity of different immune cells , we measured levels of tumour necrosis factor ( TNF alpha ) , gamma interferon ( IFN gamma ) and several interleukins ( IL 2 , IL 4 , IL 6 and IL 10 ) in children with mild ( MM ) and cerebral ( CM ) Plasmodium falciparum malaria and compared them with those of healthy children from Guadalupe Lobata District , St . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Proinflammatory cytokines , such as tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and interleukin 6 ( IL 6 ) , may be important mediators of GVHD . ^^^ Semiquantitative reverse transcriptase polymerase chain reaction was performed to amplify mRNA transcripts for TNF alpha , IFN gamma , and IL 6 using alpha32P dATP incorporation . ^^^ RESULTS : On POD 5 , there were neither clinical signs nor histologic features of GVHD , but mRNA expression of TNF alpha and IL 6 in small bowel , IL 6 in spleen , and IFN gamma in mesenteric lymph nodes were significantly increased in allograft animals when compared with normal and isograft tissues . ^^^ On POD 11 , both the clinical signs and histologic features of GVHD were seen , and TNF alpha and IL 6 in native small bowel , TNF alpha in colon , IFN gamma in spleen , and IL 6 in mesenteric lymph nodes were significantly increased in allograft animals when compared with that in normal and isograft tissues . ^^^ CONCLUSIONS : In conclusion , TNF alpha , IFN gamma , and IL 6 expression precede clinical onset and histologic evidence of GVHD in specific tissues . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The modulatory effects of interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) pretreatment of keratinocytes were investigated by adding IFN gamma or TNF alpha into the flasks of target cells 48 hours before the cytotoxicity assays . ^^^ Pretreatment of target cells with IFN gamma and TNF alpha increased the killing mediated by LAK , but had little effect on NK activity . ^^^ IL 2 , IFN gamma and TNF alpha play some critical roles in the regulation of the susceptibility of cervical keratinocytes , especially HSIL to LAK mediated cytotoxicity in vitro . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using in situ hybridization with radiolabelled oligonucleotide probes , we studied the mRNA expression of IL 1beta , IL 4 , IL 6 , IL 10 , IL 12 , tumour necrosis factor alpha ( TNF alpha ) , TNF beta , interferon gamma ( IFN gamma ) , and transforming growth factor beta ( TGF beta ) in the brain during the lethal course of experimental meningitis in a rat model inoculated intracisternally with Haemophilus influenzae type b ( Hib ) or Streptococcus pneumoniae and in uninfected control rats inoculated with the same volume of PBS . ^^^ In the brain of Hib inoculated rats , there was marked mRNA expression of IL 1beta , IL 6 , TNF alpha , IL 12 and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , cytokine mRNA present at the time of biopsy was determined by rapid RNA extraction from EOM and reverse transcription amplification with specific cytokine oligonucleotide probes ( IL 1alpha , IL 1beta , IL 2 , IL 4 , IL 6 , IL 8 , IL 10 , IL 12 , IL 13 , IL 15 , interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Superantigen activation of immune cells evokes epithelial ( T 84 ) transport and barrier abnormalities via IFN gamma and TNF alpha : inhibition of increased permeability , but not diminished secretory responses by TGF beta 2 . ^^^ Neutralizing Abs to IFN gamma partially prevented the transport abnormalities , and Abs to TNF alpha inhibited the increase in epithelial permeability . ^^^ We conclude that SAgs can elicit epithelial irregularities characteristic of enteric inflammation and that IFN gamma and TNF alpha are key mediators in this coculture model of epithelial dysfunction . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Previous studies showed that mouse spleen cells produced IL 12 , TNF alpha , and IFN gamma when stimulated with phagocytosable size chitin particles ( N acetyl D glucosamine polymers ) . ^^^ We found that these particles induced IL 12 , TNF alpha , and IFN gamma . ^^^ Finally , spleen cells from C3H / HeJ mice also showed comparable levels of IL 12 / TNF alpha / IFN gamma production when induced by 1 to 10 microm chitin particles . ^^^ Taken together , our results indicate that mannose receptor mediated phagocytosis , but not the receptor mediated pinocytosis , is highly associated with the production of IFN gamma inducing extracellular signaling factors such as IL 12 and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The suppression of inducible mRNA levels in macrophages cultured for 24 h with ES products varied with the nature of the stimuli ; IFN gamma / LPS induced iNOS mRNA levels were effected less than were iNOS mRNA levels induced by IFN gamma / IL 2 or IFN gamma / TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Ig CAM and MHC 1 expression on undifferentiated ES cells was not up regulated by treatment of cells with interferon gamma ( IFN gamma ) , tumor necrosis factor alpha , or flavivirus infection , agents that up regulate these molecules in other embryonic cell types . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of cytokines interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and interleukin 1 alpha ( IL 1 alpha ) on the release of prelabeled [ 3H ] AA were then tested in cells stably transfected with vector alone as well as cells transfected with cPLA 2 antisense plasmid . ^^^ IFN gamma ( 300 U / ml ) , TNF alpha ( 20 ng / ml ) , and IL 1 alpha ( 20 ng / ml ) all induced a significantly increased release of prelabeled [ 3H ] AA after 15 min to 2 h of treatment in control cells , and their effects were significantly reduced in cells transfected with cPLA 2 antisense vector . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Apoptosis was induced in cultured macrophages or macrophage cell lines after infection in vitro with the RH strain , whereas apoptosis was prevented when HSP 65 was induced in these cells , before infection , by activation with IFN gamma and TNF alpha . ^^^ However , apoptosis associated with infection by T . gondii RH strain was not prevented when HSP 65 synthesis was inhibited by introducing an antisense oligonucleotide for this protein into the cells before activation with IFN gamma plus TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found that tumor necrosis factor alpha ( TNF alpha ) induced de novo production of RANTES , and interferon gamma ( IFN gamma ) synergistically increased the TNF alpha dependent production of RANTES from WK hC cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We compared the antitumor effect of several transgene expression plasmids encoding specific cytokines , including interleukin 2 ( IL 2 ) , IL 4 , IL 6 , IL 12 , interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and granulocyte macrophage colony stimulating factor ( GM CSF ) , following gene gun mediated DNA delivery into the epidermis overlying an established intradermal murine tumor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It has been demonstrated that interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) treatment of a human submandibular gland ( HSG ) cell line causes growth arrest in the G0 / G1 phase of the cell cycle , followed by apoptosis . ^^^ Similarly , AdE2F1 infection of HSG cells arrested by IFN gamma and TNF alpha treatment caused a fivefold increase in S phase cells by 48 hpi . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mice primed with M CSF induced IL 6 in response to a 5 fold lower dose of LPS , and also produced higher levels of IL 1 alpha , IL 10 , GM CSF , TNF alpha , and IFN gamma than control mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
At nine months Con A induced TNF alpha secretion decreased significantly below baseline but IFN gamma secretion increased above baseline . ^^^ After nine months on treatment the CD 3 induced TNF alpha and IFN gamma secretion was not significantly different from the original baseline values . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Of the various cytokines , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and phorbol myristate acetate ( PMA ) strongly upregulated ICAM 1 protein expression on RCC . ^^^ In order to investigate whether increased gene transcription contributes to ICAM 1 upregulation , RCC cells were treated with TNF alpha , IFN gamma , or PMA with or without simultaneous addition of actinomycin D . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Thus , cachexia has been described in mice inoculated with tumor cells carrying and expressing genes for either TNF , interleukin 6 ( IL 6 ) , leukemia inhibitory factor ( LIF ) , ciliary neurotrophic factor ( CNTF ) and interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Endocrinol . ( 1994 ) 101 , R 31 R35 ) that the proinflammatory cytokines , tumor necrosis factor alpha ( TNF ) , interleukin 1 beta ( IL 1 beta ) , and interferon gamma ( IFN gamma ) , have a marked inhibitory effect on the expression and activity of type 1 iodothyronine deiodinase ( D 1 ) in FRTL 5 rat thyroid cells , while the anti inflammatory cytokine , transforming growth factor beta 1 ( TGF beta 1 ) had no effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Partial cDNA clones for the woodchuck CD3epsilon marker of T cells ( 536 bp ) and for selected woodchuck cytokines were developed , including IL 1beta ( 332 bp ) , IL 2 ( 249 bp ) , IL 4 ( 205 bp ) , IL 10 ( 476 bp ) , IFN gamma ( 476 bp ) , and TNF alpha ( 381 bp ) . ^^^ TH 2 , IL 4 / IL 10 ) , and effector molecules that regulate hepadnavirus replication and liver injury ( IFN gamma , TNF alpha ) . ^^^ The corresponding RNAs were also detectable in most cases in the total RNA from the livers of uninfected and WHV infected woodchucks and differential expression of IFN gamma and TNF alpha RNAs was suggested . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These modulatory effects of IFN gamma and TGF beta on MAPK activation correlated with changes in Fc gamma R stimulated TNF alpha production by these two cytokines . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the New Zealand Black / New Zealand White / F1 model , AS 101 significantly increased serum TNF alpha and IFN gamma while decreasing IL 10 levels ; these changes were accompanied by a rapid decrease in anti dsDNA and anti ssDNA Igs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha , IL 6 , IFN gamma , and granulocyte / macrophage ( GM ) CSF , as well as very low levels of IL 2 , were detected in TB pleural fluids . ^^^ Recombinant TNF alpha and IL 6 stimulated HIV replication , while IFN gamma and GM CSF had a more ambiguous role . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lysis and secretion of IFN gamma , and for most clones ' secretion of TNF alpha , required lower Ag densities , by one or two logs , than IL 2 and granulocyte macrophage CSF secretion . 3 ) In a significant fraction of IFN gamma secreting cells , IL 2 production is not induced . 4 ) A large fraction of cloned cells is refractory to lymphokine gene activation for about 2 wk after previous stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast to the infective parasites ( metacyclic promastigotes ) , the procyclic promastigotes collected at the logarithmic phase of the culture displayed a striking ability to induce IL 12 , IFN gamma , TNF alpha , and IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Elevated levels of human TNF alpha were accompanied by increases in synovial cell expression of murine IL 1beta and IL 6 , but murine granulocyte macrophage CSF , IFN gamma , and IL 4 could not be detected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , anti SLAM mAbs increased the production of IL 10 , IFN gamma , and TNF alpha by in vitro activated synovial fluid mononuclear cells , supporting the idea that signaling through SLAM may play a role in the regulation of synovial inflammation in patients with RA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokines examined include IL 2 , IFN gamma , TNF beta / LT , IL 4 , IL 5 , IL 10 , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of mRNAs coding for a number of cytokines ( IL 1beta , IFN gamma , TNF alpha , granulocyte macrophage ( GM ) CSF , IL 12 ( p 40 ) , and lymphotoxin beta ) was increased in tuberculous and sarcoid granulomas compared with that of control tissues . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 8 secretion from normal human epidermal keratinocytes was augmented by TNF alpha , or synergistically by TNF alpha and IFN gamma . 1 , 25 Dihydroxyvitamin D 3 and 22 oxacalcitriol inhibited cytokine stimulated IL 8 production dose dependently after 24 h incubation without inhibition of cell proliferation . 1 , 25 Dihydroxyvitamin D 3 and 22 oxacalcitriol are considered to inhibit the proliferation of keratinocytes directly and indirectly with inhibition of the secretion of IL 8 from keratinocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma can prime macrophages to transcribe and to secrete greater amounts of TNF and IL 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CD 69 expression as determined by flow cytometry was not constitutive but was induced by stimulation with interferon gamma ( IFN gamma ) plus bacterial lipopolysaccharide ( LPS ) or tumor necrosis factor a ( TNF alpha ) . ^^^ Stimulation of macrophages with anti CD 69 monoclonal antibody in the presence of IFN gamma induced both nitric oxide production and TNF alpha release . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Murine NK cells were cultured in the presence of P 815 ( H 2 Dd ) , and supernatants were analyzed for the production of interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and granulocytemacrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lipopolysaccharide ( LPS ) , interleukin 1beta ( IL 1beta ) , IL 6 , and tumor necrosis factor alpha ( TNF alpha ) , but not interferon gamma ( IFN gamma ) , suppressed the expression of P 450 2C11 mRNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the lungs of such mice persistently infected with MCMV , the mRNA of the cytokines such as IL 2 , IL 6 , TNF alpha , and IFN gamma were abundantly expressed 3 h after the anti CD 3 injection , and the elevated levels continued thereafter . ^^^ A marked expression of inducible nitric oxide synthetase ( iNOS ) was then noted in the lungs , suggesting that such cytokines as TNF alpha and IFN gamma may have induced iNOS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Macrophages and NK cells interact by way of IL 12 and TNF , which induce the NK cell to produce IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We also found a constitutively low expression of major histocompatibility complex ( MHC ) class 2 by cultured astrocytes which was significantly enhanced by lipopolysaccharide ( LPS ) , interferon gamma ( IFN gamma ) , or tumor necrosis factor alpha ( TNF alpha ) treatments . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The numbers of cytokine mRNA expressing cells for interleukin 1 ( IL 1 ) , IL 4 , IL 6 , IL 10 , IL 12 , tumor necrosis factor alpha ( TNF alpha ) , TNF beta , transforming growth factor beta ( TGF beta ) and interferon gamma ( IFN gamma ) were determined in the kidneys and spleens from the infected , non infected but obstructed and untouched mice using in situ hybridization with radio labelled oligonucleotide probes at 12 h , 48 h and 6 d after release of the urethral obstruction . ^^^ A high proportion of splenocytes further expressed mRNA for IL 6 , TNF alpha , IL 4 , IL 10 , IFN gamma and TGF beta , again with highest numbers in the infected group of animals . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This review concentrates on various cytokines ( IL 1 , TNF alpha , IFN gamma , IL 12 , IL 10 and the colony stimulating factors ( CSF ) ) thought to play a role during the innate host response to L . monocytogenes infection , with a special emphasis on studies using IL 6 deficient mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neutralization of TNF alpha in IFN gamma knockout mice mice treated with IL 12 was accompanied by the same immunohistochemical changes , implying that neither IFN gamma nor TNF alpha was required . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In spite of reduced BAL levels of TNF alpha in IL 12 treated animals , BAL levels of IFN gamma were elevated sixfold , indicative of the expected biologic response to IL 12 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Type 1 ( IFN gamma / TNF dominant ) and 2 ( IL 4 / IL 5 dominant ) granulomatous inflammation were analyzed in mice with knockout of IFN gamma or IL 4 genes . ^^^ IFN gamma knockout converted the PPD response to a type 2 like pattern with eosinophil infiltration and decreased TNF and RANTES , but increased IL 4 , IL 5 , IL 10 , IL 13 , monocyte chemoattractant protein 3 ( MCP 3 ) , and eotaxin expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Compared with chimeric OKT 3 IgG1 , IgG 2 , IgG 3 , and IgG 4 , the IgG 2 mutants were less mitogenic to T cells , and they did not induce the release of TNF alpha , IFN gamma , or IL 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
V delta 1 T cells expanded in the blood throughout HIV infection display a cytotoxic activity and are primed for TNF alpha and IFN gamma production but are not selected in lymph nodes . ^^^ In addition , single cell analysis of cytokine production revealed that , in contrast to 5 delta 1 T cells from control donors , patients ' 5 delta 1 T cells are primed in vivo for IFN gamma and TNF alpha production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokines IL 1 , IL 2 , IFN beta , IFN gamma , and TNF alpha , known to activate T cells , did not affect CD 15 expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma , IL 1 alpha , TNF alpha ) to protect against bacterial infection , and illustrating the rapidity of the host cytokine response ( detectable within 1 h of challenge ) during bacterial infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Extensive studies of the molecular basis of the latter phenomenon led to the conclusion that the most decisive step from non specific microabscess formation to granulomatous inflammation is the activation of non specifically invading CD4+ T cells , which results in high local concentrations of TNF alpha and IFN gamma in the presence of IL 2 . ^^^ Because any attempt to neutralize the effects of TNF alpha and IFN gamma to modulate T cell mediated inflammation will also dramatically decrease host resistance , other anti inflammatory strategies based on the modulation of TNF alpha and IFN gamma induced mechanisms of monocyte accumulation must be developed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of IL 1 alpha , IL 1 beta , IL 2 , IL 4 , IL 6 , IL 10 , IL 12 , IFN gamma , TGF beta , TNF alpha , and PDGF was examined in 28 patients with IgA nephropathy ( IgAN ) , 20 patients with non IgA mesangial proliferative glomerulonephritis ( mesPGN ) , and 19 healthy controls . ^^^ Furthermore , the renal profile of IL alpha , IFN gamma , and TNF alpha expression was associated with the expression of IFN gamma in PBMC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CD3+ CD4+ TIL displayed a normal capacity to up regulate CD 25 expression and to secrete both Th 1 type ( IL 2 , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) ) and Th 2 type ( IL 4 , IL 5 and IL 10 ) cytokines upon triggering with anti CD 3 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , we detected up regulation of local cytokine genes ( IL 1beta , tumour necrosis factor alpha ( TNF alpha ) , IL 2 , interferon gamma ( IFN gamma ) , IL 6 , IL 10 , and IL 12 p 40 ) during the course of autoimmune dacryoadenitis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and interleukin 2 ( IL 2 ) were labeled with 125Iodine for quantitative transport studies using autoradiography . ^^^ Intracarotid infusion of RMP 7 ( 0 . 1 microgram kg 1 min 1 ) increased the selective transport to tumors of IFN gamma by 3 . 97 fold ( p < 0 . 005 ) , of TNF alpha by 5 . 30 fold ( p < 0 . 005 ) , and of IL 2 by 4 . 34 fold ( p < 0 . 005 ) , compared to intracarotid saline infusion . ^^^ To determine whether the increased IFN gamma or TNF alpha transport to tumors with RMP 7 could enhance expression of intercellular adhesion molecule 1 ( ICAM 1 ) in tumors , ICAM 1 expression in RG 2 glioma was evaluated by immunohistochemistry . ^^^ Both IFN gamma and TNF alpha increased ICAM 1 expression of RG 2 cells in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This was done by treating cultured Caco 2 cells , a transformed human intestinal epithelial cell line , with different concentrations of tumor necrosis factor alpha ( TNF alpha ) , IL 1 beta , IL 6 or interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study the modulatory effects of inflammatory cytokines , interleukin 1 beta ( IL 1 beta ) , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) , on CA 125 release in established ovarian cancer cell lines and in human peritoneal mesothelial cells ( HPMC ) both grown as monolayers , were investigated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Therefore , PBMC were tested for tumor necrosis factor alpha ( TNF alpha ) , interleukin 6 ( IL 6 ) , and interferon gamma ( IFN gamma ) by specific ELISA , after stimulation with phytohemagglutinin ( PHA ) or lipopolysaccharide ( LPS ) in the presence or absence of different concentrations of chloroquine or hydroxychloroquine . ^^^ RESULTS : We observed that chloroquine and hydroxychloroquine equally inhibit PHA induced TNF alpha and IFN gamma production , and LPS induced TNF alpha and IL 6 production , while PHA induced IL 6 production was not affected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Cultures of bovine corneal endothelial cells and keratocytes were exposed to increasing concentrations of LPS , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) . ^^^ RESULTS : Exposure of BCE cells and keratocytes to LPS and IFN gamma resulted in an increase of nitrite levels that was potentiate by the addition of TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines , such as interferon gamma ( IFN gamma ) , TNF alpha , and IL 1alpha , are elevated in patients ' sera . ^^^ To verify whether infection of T cells by EBV will upregulate specific cytokine genes and subsequently activate macrophages leading to hemophagocytic syndrome , we studied the transcripts of TNF alpha , IFN gamma , and IL 1alpha in EBV infected and EBV negative lymphoma tissues . ^^^ Consistent with these in vivo observations , in vitro EBV infection of T cell lymphoma lines caused upregulation of TNF alpha gene , and increased secretion of TNF alpha , but not IFN gamma or IL 1alpha . ^^^ Expression of TNF alpha , IFN gamma , and IL 1alpha was not changed by EBV infection of B cell lymphoma lines . ^^^ Enhanced phagocytosis and secretion of TNF alpha , IFN gamma , and IL 1alpha by U 937 cells were observed , and could be inhibited to a large extent by anti TNF alpha ( 70 % ) , less effectively by anti IFN gamma ( 31 % ) , but almost completely by the combination of anti TNF alpha and anti IFN gamma ( 85 % ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tick induced suppression of host immune defences is characterized by reduced ability of lymphocytes from infested animals to proliferate in vitro in the presence of concanavalin A ( Con A ) , diminished primary antibody responses to T cell dependent antigen , and decreased elaboration of macrophage ( IL 1 and TNF alpha ) and Th 1 lymphocyte cytokines ( IFN gamma ) , whereas Th 2 cytokines production ( IL 4 , IL 5 and IL 10 ) is enhanced . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inhibition of DNA synthesis in T lymphocytes , B lymphocytes , NK cells and myelomonocytic cells as well as of the production of IL 2 , IL 6 TNF alpha , IL 1 , IL 10 , IL 12 , IL 13 , IFN gamma , GM CSF are not due to apoptosis of these cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We also determined the effect of the pro inflammatory cytokines IL 1 beta , TNF alpha and IFN gamma on the expression of these molecules by RPE cells in culture . ^^^ However , incubation with 50 pg / ml of IL 1 beta , TNF alpha and IFN gamma caused a marked increase in the RPE cell expression of ICAM 1 following 4 , 24 and 48 h culture . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The short term priming effects of two cytokines , interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) , on rat PMNL in whole unfractionated blood were investigated in the present study . ^^^ One hour incubation with TNF alpha , or the mixture of TNF alpha + IFN gamma resulted in a dose dependent enhancement of ROS production in response to PMA . ^^^ Moreover , the incubation with both IFN gamma and TNF alpha caused a significantly higher increase of ROS production than with TNF alpha alone . ^^^ The results of the present study suggest that IFN gamma may enhance the priming effects of TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both systemic and intrapulmonary IFN gamma levels were significantly higher in rats depleted of TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interestingly , maximal production of IFN gamma by NK cells stimulated with IL 2 plus IFN alpha / beta was dependent on endogenous tumor necrosis factor alpha ( TNF alpha ) . ^^^ Further studies revealed that TNF alpha enhanced the ability of IFN alpha / beta to stimulate production of IFN gamma by NK cells . ^^^ This inhibitory effect was not reversed by the addition of neutralizing antibodies specific for IFN gamma or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mononuclear cells from paired blood samples from fifteen patients with myasthenia gravis before and after thymectomy and immunosuppressive therapy were examined by in situ hybridization for acetylcholine receptor induced mRNA expression of the T helper type 1 proinflammatory cytokines interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) and TNF beta , the T helper type 2 cytokines interleukin 4 ( IL 4 ) and IL 10 and the immune response downregulating cytokine tumor growth factor beta ( TGF beta ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Release of the bacteria specific cytokines interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 10 ( IL 10 ) , and IL 4 was measured in synovial fluid mononuclear cells ( SFMC ) using enzyme linked immunosorbent assay and polymerase chain reaction . ^^^ RESULTS : Upon stimulation with specific bacteria , SFMC secreted low amounts of IFN gamma and TNF alpha , but high amounts of IL 10 . ^^^ IL 10 was responsible for the suppression of IFN gamma and TNF alpha , as judged by the effect of adding either anti IL 10 antibodies or exogenous IL 10 to these cultures . ^^^ Exogenous IL 12 clearly enhanced IFN gamma and TNF alpha secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
No spontaneous IFN gamma or IL 2 were noted in sera with treatment , but production of IL 2 by MNCs rose with TNF treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferons ( IFN gamma in particular ) in cooperation with TNF alpha and IL 1 inhibit proliferation of tumor cells and by their synergic activity with IL 2 induce cytotoxicity of NK cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin 2 ( IL 2 ) , IL 12 , and IL 15 , but not IL 7 , interferon gamma ( IFN gamma ) nor tumor necrosis factor alpha ( TNF alpha ) induced functional and phenotypic maturation of CB NK cell subsets . ^^^ Studies using neutralizing anti IFN gamma and anti TNF alpha antibodies showed that survival and phenotypic maturation upon cytokine stimulation is influenced by endogenous production of TNF alpha but not IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The proinflammatory cytokine , TNF alpha , was markedly enhanced in kdkd kidney ( up to fourfold ) , whereas the T cell specific cytokine , IFN gamma , increased less ( less than twofold ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Secretion of IL 1 alpha , IL 6 and TNF alpha was measured by ELISA following maximal stimulation with LPS and IFN gamma , and cytokine mRNA expression was detected by in situ hybridization . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) and gamma interferon ( IFN gamma ) are required for an effective immune response to bacterial infection and these cytokines synergize in a variety of biological responses , including the induction of cytokine , cell adhesion , and inducible nitrous oxide synthase gene expression . ^^^ However , since activation of NF kappaB by TNF alpha is often transient and would not activate long term kappaB dependent transcription effectively , we explored the effects of IFN gamma on TNF alpha induced NF kappaB activity . ^^^ IFN gamma , which typically does not activate NF kappaB , synergistically enhanced TNF alpha induced NF kappaB nuclear translocation via a mechanism that involves the induced degradation of 1 kappaBbeta and that apparently requires tyrosine kinase activity in preneuronal cells but not in endothelial cells . ^^^ Correspondingly , cotreatment of cells with TNF alpha and IFN gamma leads to persistent activation of NF kappaB and to potent activation of kappaB dependent gene expression , which may explain , at least in part , the synergy observed between these cytokines , as well as their involvement in the generation of an effective immune response . . ^^^ Tumor necrosis factor alpha ( TNF alpha ) and gamma interferon ( IFN gamma ) are required for an effective immune response to bacterial infection and these cytokines synergize in a variety of biological responses , including the induction of cytokine , cell adhesion , and inducible nitrous oxide synthase gene expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Further , the results showed that IFN gamma mRNA was simultaneously expressed in the same embryos that also expressed mRNA markers for macrophage activation ( TNF alpha and iNOS ) , indicating that macrophage activation could be a consequence of IFN gamma production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Flow cytometric analysis ( FACS ) of the CD5 / CD19 , CD4 / CD8 , CD14 / CD3 , CD16 / CD3 , CD45RA / CD4 , and CD45RO / CD4 surface markers on PBLs was performed and the levels of the IL 1beta , IL 2 , IL 4 , IL 6 , IL 10 , TNF alpha , IFN gamma , and IL 2R were also examined . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Der p induced a significant BMC proliferation ( P < 0 . 01 ) , associated with increased expression of HLA DRag and IL 2r ( P < 0 . 05 ) and with enhanced release of IL 2 , GM CSF , IL 1 beta , TNF alpha and IFN gamma ( P < 0 . 01 , each comparison ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma acts as a mediator of multiple sclerosis ( MS ) exacerbations through a number of biological effects , such as induction of major histocompatibility class 2 complexes ( MHC 2 ) , macrophage activation and potentiation of tumor necrosis factor ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It is now known that the proinflammatory cytokines IFN gamma and tumor necrosis factor alpha ( TNF alpha ) are probably involved in the pathogenesis of MS lesions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) are proinflammatory cytokines which may be involved in the pathogenesis of MS . ^^^ IFN alpha counteracts many of the proinflammatory actions of IFN gamma and TNF alpha . ^^^ Baseline lymphocyte IFN gamma ( 19 . 10 + / 7 . 12 U ml 1 ) and TNF alpha ( 18 . 05 + / 5 . 34 pg ml 1 ) production significantly decreased to 3 . 03 + / 0 . 66 ( P < 0 . 04 ) ( for IFN gamma ) and to 5 . 78 + / 0 . 90 ( P < 0 . 04 ) ( for TNF alpha ) after rIFN alpha treatment . ^^^ IFN gamma and TNF alpha production was unchanged in the placebo group . rIFN alpha was tolerated without drop outs or serious side effects , but fever , malaise , fatigue ( interfering with daily activities in two patients ) and leukopenia frequently occurred . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Peak levels of tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) occurred at 2 h whereas the rise in interleukin 6 ( IL 6 ) was significantly delayed and peaked at 4 h after starting antibody treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Firstly , CC 16 behaves like an anti cytokine by downregulating the production of IFN gamma , IL 1 and TNF alpha by stimulated leukocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Twenty four hours after IFN beta 1b treatment , mRNA levels for IL 1 , IL 2 , IL 4 , IL 6 , IL 10 , IL 12 , IL 13 , IFN gamma , TNF alpha and LIF had not changed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation with tumour necrosis factor alpha ( TNF alpha ) upregulated expression of ICAM 1 , whereas Interferon gamma ( IFN gamma ) and a combination of IFN gamma and TNF alpha upregulated class 1 MHC antigens and ICAM 1 and induced class 2 MHC molecules . ^^^ The level of expression of MHC antigens and of ICAM 1 and LFA 3 after stimulation of HIBEC with combined IFN gamma and TNF alpha was comparable with the expression of these antigens by an Epstein Barr virus transformed B cell line . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These T cells do not secrete IL 2 or 4 but can produce IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines detected in the circulation during sepsis ( tumor necrosis factor alpha [ TNF alpha ] , interferon gamma [ IFN gamma ] , granulocyte colony stimulating factor [ G CSF ] , granulocyte macrophage colony stimulating factor [ GM CSF ] ) inhibited neutrophil apoptosis in both groups , though the effect was more distinct in neutrophils from healthy humans . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Proinflammatory cytokines like interferon gamma ( IFN gamma ) and TNF alpha can induce Fas expression and render hematopoietic progenitor cells susceptible to Fas induced growth suppression and apoptosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Purified CD4+ and CD8+ T cells from patients with clinically definite , stable relapsing MS ( RRMS ) were stimulated by anti CD 3 mAb or Con A for 48 hours and cytokine supernatants analysed for production of IL 2 , IL 6 , IFN gamma , TNF alpha ( potential pro inflammatory ) and IL 4 , IL 10 , and TGF beta ( potential counterregulatory anti inflammatory ) . ^^^ Con A activated CD4+ and CD8+ T cell proinflammatory cytokine IL 2 secretion , CD4+ T cell IL 6 secretion , CD4+ and CD8+ T cell TNF alpha secretion and CD8+ T cell IFN gamma secretion was decreased significantly in RRMS subjects compared to controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Semiquantitation of mRNA levels by competitive PCR revealed that pretreatment with gamma interferon ( IFN gamma ) amplified the expression approximately 100 fold , while pretreatment with tumor necrosis factor alpha ( TNF alpha ) or granulocyte macrophage colony stimulating factor augmented this expression about 10 fold . ^^^ Unexpectedly , the addition of three different kinds of anti IFN gamma antibodies and anti IFN gamma receptor antibody and the coaddition of anti TNF alpha antibody with anti IFN gamma receptor antibody all failed to inhibit IL 12 mRNA expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
If interferon gamma ( IFN gamma ) is added to the cell culture medium together with TNF alpha the surface expression of E selectin is augmented and prolonged in a synergistic way . ^^^ Lectin blot analysis using the Sambucus nigra agglutinin lectin also indicated differences in protein glycosylation when HUVEC were incubated with IFN gamma / TNF alpha compared to TNF alpha alone . ^^^ When HUVEC were incubated with monesin , a potent inhibitor of late Golgi function , together with both TNF alpha and IFN gamma , the additive effect of IFN gamma on E selectin expression was almost abolished . ^^^ However , when HUVEC were cultured with IFN gamma / TNF alpha in the presence of several different inhibitors of N glycosylation processing , no significant effect of E selectin expression was observed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Macrophage treatment with either IFN gamma or TNF alpha had no effect . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OBJECTIVE : Interleukin 6 ( IL 6 ) , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) are important mediators of the inflammatory response in human infection . ^^^ The aim of this study was to determine the relationship between serum levels of IL 6 , TNF alpha , IFN gamma and CRP in febrile children with malignant disease , and relate these levels to aetiology of fever , presence of neutropenia and the effect of untreated malignancy . ^^^ IFN gamma was elevated in 18 % of cases and correlated strongly with mean TNF alpha levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
All cell lines displayed a lack of sensitivity to TNF alpha , although some degree of sensitivity to TNF alpha could be observed in the presence of actinomycin D or after treatment with IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum IL 10 , interferon gamma ( IFN gamma ) , soluble CD 23 ( sCD 23 ) , IL 8 , granulocyte macrophage colony stimulating factor ( GM CSF ) , IL 1beta and tumour necrosis factor alpha ( TNF alpha ) were measured using in house ELISAs . ^^^ Regardless of steroid therapy or disease activity , serum levels of IFN gamma , TNF alpha , IL 1beta , GM CSF and IL 8 were no different in patients with sarcoidosis and controls . ^^^ Consistent with a quiescent peripheral immune system , factors capable of monocyte / macrophage activation ( TNF alpha , IFN gamma , GM CSF and IL 8 ) were not elevated in the peripheral circulation . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Animals in which the tumour regresses show high levels of cytokines in the serum compared with animals which succumb to the tumour , and levels of IL 2 , IL 4 , IL 12 , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) are significantly higher in tumour tissue of the former . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma also potentiates production of cytokines such as tumor necrosis factor alpha ( TNF alpha ) , interleukin 1beta ( IL 1beta ) and IL 12 . ^^^ We also compared the effects of IFN gamma and IL 10 on production of TNF alpha and sTNF RII by these cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum concentrations of IFN alpha , IL 1 alpha , IL 1 beta , TNF alpha , and IFN gamma were measured at intervals throughout the study period . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Once the macrophage has been activated by IFN gamma and TNF alpha the vacuoles coalesce and acidify . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Quinolinic acid production by macrophages stimulated with IFN gamma , TNF alpha , and IFN alpha . ^^^ Results at 72 h showed significant increases in QUIN production in the cells stimulated with IFN gamma ( 10297 + / 170 nmol / L ) and also in those stimulated with IFN alpha ( 3600 + / 113 nmol / L ) , whereas TNF alpha stimulated macrophages produced low levels of QUIN ( 1108 + / 23 nmol / L ) . ^^^ Macrophages stimulated with the cytokine combinations TNF alpha and IFN gamma , IFN alpha , and IFN gamma , and TNF alpha and IFN alpha also resulted in increases in QUIN production ( 11471 + / 77 . 6 nmol / L , 16656 + / 184 nmol / L , and 3369 + / 120 . 5 nmol / L , respectively ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Secretion of TNF alpha , IL 6 , and IFN gamma was considered as a marker of BAL or PBL cell activation . ^^^ It was established that AE 22 can induce TNF alpha , IL 6 , and IFN gamma in a dose dependent manner in BAL cells and PBL isolated from healthy individuals . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Langerhans cell histiocytosis ( LCH ) is a clonal proliferation of dendritic histiocytes expressing elevated levels of tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 1 ( IL 1 ) , and leukemia inhibitory factor ( LIF ) . ^^^ The TNF alpha and IFN gamma promoter DNA sequences of 12 LCH patients were studied and compared with normal individuals by dideoxy fingerprinting and DNA sequencing . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Low level transcription of IFN gamma , IL 4 , IL 1 beta , and TNF alpha was typical of diffuse synovitis . ^^^ Granulomatous synovitis demonstrated high transcription of IFN gamma , IL 4 , IL 1 beta , and TNF alpha and could be clearly distinguished from the other phenotypes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines were selected on biological plausibility and measured both in a whole blood assay ( TNF alpha , IL 8 ) and in serum ( IL 4 , IL 5 , IL 6 , IFN gamma ) . ^^^ The hypothesis is that blood levels of TNF alpha and IL 8 are increased in bronchitis , while serum levels of IL 4 , IL 5 are increased and IFN gamma is decreased in asthmatic workers . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The prevalence of IL 2 , IFN gamma , and TNF beta producing T cells was not reduced by CNI 1493 . ^^^ Phorbol ester and ionomycin activation also resulted in a CNI 1493 induced inhibition of TNF alpha in monocytes but resistant production of TNF alpha , IL 2 , and IFN gamma by T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The following parameters were evaluated monthly : 1 ) hemoglobin and hematocrit values ; 2 ) serum levels of erythropoietin ( EPO ) ; and 3 ) serum levels of interleukin ( IL ) 3 , IL 6 , IL 10 , IL 1 beta , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) . ^^^ At the same time , an increase in serum IL 3 , IL 6 , and IL 10 levels was seen , whereas serum IL 1 beta , TNF alpha , and IFN gamma levels decreased . ^^^ In HD patients , EPO resistance may caused either by absorption of rHuEPO to the cuprophan M , or an increased release of cytokines that inhibit erythropoiesis , such as IL 1 beta , TNF alpha , and IFN gamma , and to a decrease in stimulatory cytokines such as IL 3 , IL 6 , and IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We assayed bronchoalveolar lavage ( BAL ) fluid from HIV positive and normal individuals to determine if increased levels of TNF alpha and IFN gamma were present in the lungs of HIV infected individuals . ^^^ METHODS : Transendothelial migration was assessed by determining the number and flow cytometric phenotype of PBMC adherent to or migrating across unstimulated or TNF alpha and IFN gamma activated endothelial cell monolayers . ^^^ We measured BAL fluid cytokine concentrations using standard antigen capture enzyme linked immunosorbent assays for TNF alpha and IFN gamma . ^^^ CD8+ T cell extravasation is aided by TNF alpha and IFN gamma induced endothelial cells activation . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This enhancement occurred within a few hours of exposure to the virus and was blocked by Abs to IL 15 , but not to other cytotoxicity enhancing cytokines ( i . e . , to IFN gamma , IL 2 , TNF alpha , or IL 12 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The physiopathology of experimental cerebral malaria ( CM ) , an acute neurological complication of Plasmodium berghei ANKA ( PbA ) infection , involves interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) , two cytokines that are known to modulate major histocompatibility complex ( MHC ) molecule expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
T helper ( Th ) 1 related cytokines ( IL 2 , IFN gamma , and TNF beta ) were more strongly expressed than Th 2 related cytokines ( IL 4 and IL 10 ) in vivo , indicating that the Th cells which infiltrated the heart and mediated the immune responses in the early phase of acute myocarditis were mainly of Th 1 type . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cationic liposomes , irrespective of their DNA content , downregulated NO and TNF alpha synthesis by lipopolysaccharide ( LPS ) / interferon gamma ( IFN gamma ) activated macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha significantly enhanced WNV induced increases in E selectin , P selectin , ICAM 1 , and VCAM 1 expression , while IFN gamma enhanced WNV induced ICAM 1 expression . ^^^ IFN gamma , TNF alpha , or IL 1 acted in synergy with WNV to produce greater increases in MHC 1 expression than WNV or cytokines alone , while IFN alpha / beta or IL 4 had no effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vivo and in vitro analyses revealed that enriched NK cells from day 6 infected A / J mice were defective not only in NKCC , but also in IFN gamma , and to a certain extent , TNF alpha secretion , which could also be corrected by IL 12 treatment . ^^^ NKCC defective bg / bg mice produced optimum IFN gamma and TNF alpha and recovered from infection similar to bg / + controls ; in vivo depletion of these cytokines resulted in significantly higher parasitemia early in infection . ^^^ Based on these results , we conclude that IFN gamma , and possibly TNF alpha , secretion by NK cells during early infection plays a major role in protective immunity to blood stage malaria . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Simultaneous addition of TNF alpha restored the level of NO synthesis by RMG and RPE cells from LT alpha / TNF alpha knockout mice stimulated with LPS and IFN gamma to wild type levels . ^^^ Transforming growth factor beta ( TGF beta ) blocked LPS / IFN gamma induced NO production is RMG and RPE cells from wild type and LT alpha / TNF alpha knockout mice . ^^^ Our results demonstrate that induction of NO synthesis in RMG and RPE cells by LPS and IFN gamma is dependent in part on endogenous TNF alpha while inhibition of NO production by TGF beta does not require a modulation of TNF alpha synthesis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Malaria toxins ( MT ) of both plasmodium species induced cell proliferation and the production of IFN gamma in overnight and long term ( 5 days ) spleen and bone marrow cultures and a reduction of the number of TNF alpha spot forming cells ( SFC ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When stimulated through IgE ( or IgG ) immune complexes with parasite antigens , mast cells can release several cytokines , including IL 4 , IL 6 , IL 10 , IL 12 , Interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) that may influence the host response to Leishmania major in modulating lesion size and persistence during experimental infection in the mouse . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
On day 5 , IL 12 treated mice had elevated serum and spleen IFN gamma and tumor necrosis factor ( TNF ) . ^^^ The IL 12 predose also inhibited IL 12 induced IFN gamma levels , but did not modify IL 12 induced CS , TNF or sTNFR P 75 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 15 significantly enhanced T cell production of interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and IL 10 . ^^^ Exogenously administered IL 10 significantly reduced the IL 15 and IL 2 mediated IFN gamma and TNF alpha production , whereas T cell proliferation and CD 25 expression were not affected . ^^^ Inhibition of endogenous IL 10 binding to the IL 10 receptor significantly increased IFN gamma and TNF alpha release from T cells . ^^^ These data suggest that endogenous IL 10 can regulate activated T cell production of IFN gamma and TNF alpha via a paracrine negative feedback loop . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Addition of TNF alpha ( 10 ng / ml ) and IFN gamma ( 20 ng / ml ) increased the proportion of TUNEL positive cells to 30 % , whereas the cytokines alone were without effect . ^^^ Addition of TNF alpha and IFN gamma enhanced cytotoxicity and resulted in a higher proportion of apoptotic cells , suggesting that inflammatory cytokines can increase the cytotoxicity of lipid oxidation products . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , IFN gamma and IL 2 could prevent TNF alpha mediated apoptosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NO within the same cell , the timing of inducible nitric oxide synthase ( iNOS ) and EC SOD transcription was measured after exposure of a rate type 2 pneumocyte analog , the L 2 cell line , to a combination of interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^ Both EC SOD and iNOS were elevated in rat lung homogenates 24 h after intratracheal instillation with IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , 120 days p . i . , IFN gamma and TNF alpha mRNA were approaching baseline levels in TG of acyclovir treated mice , but remained significantly elevated in untreated controls ( i . e . , IFN gamma mRNA levels were sixfold higher in TG of untreated mice ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , when 8 wk old scid mice were immunologically reconstituted , signs of intense , focal pulmonary inflammation were observed , and levels of interleukin ( IL ) 1alpha , IL 1beta , IL 3 , IL 6 , interferon gamma ( IFN gamma ) , tumor necrosis factor ( TNF ) alpha , and TNF beta mRNAs were all significantly elevated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
ACD3S contained increased levels of interleukins ( IL ) 1 , 2 , 6 , 7 and 12 , as well as of granulocyte macrophage colony stimulating factor ( GM CSF ) , gamma interferon ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 15 also significantly induced interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) protein production ( days 1 , 3 , and 6 , P < . 05 , n = 3 ) in CB MNCs . ^^^ The reduced production of IL 15 from CB MNCs in response to stimulation may contribute to the decrease in IFN gamma and TNF alpha production and CB cellular immunity . ^^^ However , exogenous IL 15 enhanced IFN gamma and TNF alpha production and NK and LAK cytotoxicities in CB MNCs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 10 inhibited PbA antigen specific interferon gamma ( IFN gamma ) production in vitro but not tumour necrosis factor ( TNF ) serum levels in vivo . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Generation of dendritic cells from bone marrow progenitors using GM CSF , TNF alpha , and additional cytokines : antagonistic effects of IL 4 and IFN gamma and selective involvement of TNF alpha receptor 1 . ^^^ We report the generation of dendritic cells ( DC ) starting from CD34+ bone marrow ( BM ) progenitor cells , using a two stage culture system in which , besides granulocyte macrophage colony stimulating factor ( GM CSF ) and tumour necrosis factor alpha ( TNF alpha ) , stem cell factor ( SCF ) was added during the first 5 days , while interleukin 4 ( IL 4 ) and / or interferon gamma ( IFN gamma ) were added during the secondary culture period of 9 days . ^^^ An antagonism between IL 4 and IFN gamma could further be confirmed in that , as compared with IL 4 alone , the simultaneous addition of IL 4 and IFN gamma to GM CSF plus TNF alpha during maturation reduced both the phenotypical ( CD1a , CD 4 , CD 40 ) and functional characteristics of DC . ^^^ We conclude first , that the expansion of DC from BM progenitors could most effectively be enhanced in a two stage culture assay using SCF , GM CSF , TNF alpha and IL 4 ; second , that the effect of TNF alpha in DC generation involves signalling via the TNF R 1 receptor ; and third , that IFN gamma counteracts some of the effects of IL 4 in DC generation . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
They further show that peripheral blood gamma delta T cells may function as a source of the proinflammatory cytokines IFN gamma and TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After LPS challenge to BALB / c nude mice , their livers expressed IL 12 mRNA , followed by the induction of IFN gamma and FasL mRNA and then by the late elevation of TNF alpha mRNA , but stably expressed IL 18 mRNA . ^^^ The in vivo administration of anti IL 18 just before an LPS challenge suppressed not only the induction of IFN gamma and the late TNF alpha elevation , but also the FasL induction , resulting in the total prevention of liver injury , whereas such an anti IL 12 treatment did not . ^^^ Anti IFN gamma treatment reduced the late increase in TNF alpha , but not FasL , resulting in a partial prevention of the liver injury . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The inhibitory effect of IFN gamma on poly ( 1 : C ) induced E selectin was concentration and time dependent and was specific for dsRNA , in that the induction of E selectin by TNF alpha , IL 1 beta , thrombin , or LPS was not inhibited significantly by this pretreatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
By depleting Brown Norway ( BN , RT1n ) rat lung allografts of AM before transplantation into Lewis rat ( LEW , RT 1 ( 1 ) ) recipients , the current study determined the role of donor AM in including the production of TNF alpha , IFN gamma ( Th 1 cytokine ) , IL 4 ( Th 2 cytokine ) , IgG subtypes , and rejection pathology in the allograft . ^^^ The data show that compared with untreated BN allografts , pretransplant depletion of donor lung AM resulted in significantly less TNF alpha , and IFN gamma production in allograft bronchoalveolar lavage fluid with variable effects on local IL 4 production . ^^^ These data show that donor AM have a central role in the local synthesis of TNF alpha and induce the production of IFN gamma and IgG subtypes , locally , during acute lung allograft rejection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Concentration of selected cytokines in subretinal fluid in patients with retinal detachment ( IL 8 , TNF alpha , IFN gamma ) ] . ^^^ PURPOSE : The aim of this study was to evaluate the presence of IL 8 , TNF alpha , IFN gamma in subretinal fluid of patients with rhegmatogenous retinal detachment . ^^^ RESULTS : IL 8 , TNF alpha and IFN gamma were found in all subretinal fluid samples . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Subsequent studies have shown that the protective immune mechanisms were dependent on antigen specific CD4+ T cells , the activation of alveolar macrophages , the recruitment and activation of polymorphs , predominantly neutrophils , the controlled secretion of TNF alpha , IL 1 and IFN gamma and the presence of antibody . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) has been shown to inhibit interleukin 1 ( IL 1 ) and tumor necrosis factor alpha ( TNF alpha ) stimulated bone resorption by strongly stimulating nitric oxide ( NO ) synthesis . ^^^ Stimulation of the cocultures with IL 1 beta , TNF alpha and IFN gamma markedly enhanced NO production by 50 to 70 fold , and this was found to be derived predominantly from the osteoblast cell layer . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our results show that an increase in IL 2 , IFN gamma , IL 4 , IL 12 and TNF alpha contributed to early regression , suggesting a predominantly Th 1 type of cytokine function being evoked against AK 5 tumor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Induction of death ( CD95 / FAS ) , activation and adhesion ( CD 54 ) molecules on blast cells of acute myelogenous leukemias by TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We measured the activity of adenosine deaminase ( ADA ) and the concentration of interleukin 1 beta ( IL 1 beta ) , interleukin 2 ( IL 2 ) , interleukin 8 ( IL 8 ) , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) in the pleural effusions from 28 patients with tuberculosis , 30 with neoplastic diseases , 25 with bacterial infections and 18 with congestive heart failure or liver cirrhosis . ^^^ The cut off points of ADA , IL 1 beta , IL 8 , TNF alpha and IFN gamma determined in this analysis were 54 IU / L , 5 . 5 pg / ml , 405 pg / ml , 4 . 5 pg / ml and 28 pg / ml , respectively and the sensitivity and the specificity of them were 88 . 0 % and 95 . 9 % , 19 . 1 % and 74 . 1 % , 57 . 1 % and 63 . 2 % , 81 . 0 % and 77 . 2 % , and 96 . 2 % and 98 . 5 % , respectively . ^^^ In ADA , TNF alpha and IFN gamma , the areas under the curve ( AUC ) that represent the diagnostic accuracy were 0 . 968 , 0 . 719 and 0 . 993 , respectively . ^^^ AUC of IFN gamma was significantly higher than that of ADA or TNF alpha . ^^^ In tuberculous pleural effusion , IFN gamma was significantly correlated with TNF alpha , IL 1 beta and ADA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our findings indicate a way by which TNF alpha , as well as LPS , IFN gamma , aspirin , and ox LDL impinge upon macrophage biology . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cerebrospinal fluid ( CSF ) and serum levels of interleukin ( IL ) 1beta , IL 6 , and tumor necrosis factor alpha ( TNF alpha ) , their messenger RNA expression in brain areas ( hypothalamus , hippocampus , and striatum ) and in spleen were evaluated 2 and 8 h after endotoxin [ lipopolysaccharide ( LPS ) , 25 microg / rat i . c . v . ] , IFN gamma ( 2 . 5 microg / rat i . c . v . ) or after their coadministration in rats . ^^^ IFN gamma potentiated LPS effect on IL 6 and TNF alpha levels in both CSF and serum . ^^^ LPS and IFN gamma coadministration did not alter IL 1beta messenger RNA expression induced by LPS in brain areas and in spleen , but it potentiated that of IL 6 and TNF alpha . ^^^ The present in vivo data show that i . c . v . coadministration of LPS and IFN gamma results in a potentiation of cytokine production ( IL 6 and TNF alpha ) which may trigger a cascade of events relevant to neurodegenerative processes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Impaired response to IFN gamma was documented in B cells by signal transducer and activator of transcription 1 nuclear translocation , in fibroblasts by cell surface HLA class 2 induction , and in monocytes by cell surface CD 64 induction and TNF alpha secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Dexamethasone and noradrenaline suppressed secretion of IL 2 , IFN alpha , IFN gamma , TNF alpha , IL 1 alpha and IL 1 beta . beta Endorphin and Leu Enkephalin had no significant influence on cytokine secretion . ^^^ GM CSF stimulated the secretion of IL 1 alpha , IL 1 beta and TNF gamma , but had no influence on the secretion of IL 2 , IFN alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CVL fluid was assayed for levels of IgG , secretory IgA ( s IgA ) , interleukin 2 ( IL 2 ) , IL 10 , IL 6 , tumor necrosis factor alpha ( TNF alpha ) , IL 1beta , interferon gamma ( IFN gamma ) , C 3 , C1q , and C 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A panel of monoclonal antibodies against cytokines applied to these tissues clearly detected tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , interleukin 1beta ( IL 1beta ) , and IL 10 in the tissues from brain and liver of case 1 . ^^^ A different cytokine profile , IL 4 and IL 10 , was found in the brain tissues of case 2 ; no TNF alpha nor IFN gamma was detected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Release of tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , IL 6 , IL 8 , IL 10 , soluble ( s ) CD 4 , sCD 8 and sCD 25 by PBMC was equal under both conditions and could be used as an indicator for T cell activation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , SEB induced IL 2 and IL 10 mRNA expression was suppressed only by CsA , whereas tumour necrosis factor alpha ( TNF alpha ) was decreased only by anti IFN gamma Abs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To explore the mechanisms of immuno modulatory activities of bleomycin , we investigated interferon gamma ( IFN gamma ) mRNA expression , tumor necrosis factor alpha ( TNF alpha ) production , nitric oxide ( NO ) production and macrophage tumoricidal activities in rats bearing KDH 8 hepatoma cells , which secreted a large amount of transforming growth factor beta ( TGF beta ) , and these processes in KDH 8 tumor bearing rats treated with bleomycin . ^^^ We found that IFN gamma mRNA expression , TNF alpha production , NO production and macrophage cytotoxic activities were lower in the KDH 8 bearing rats than in normal rats . ^^^ On the other hand , low dose bleomycin restored the macrophage cytotoxic activities , NO production , IFN gamma mRNA expression and TNF alpha production in the KDH 8 bearing rats . ^^^ In vitro experiments showed that KDH 8 derived TGF beta decreased the IFN gamma mRNA expression and TNF alpha production in splenocytes , and NO production in peritoneal macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Levels of pro inflammatory cytokines , such as tumor necrosis factor alpha , interleukin ( IL ) 1 beta and IL 6 , in the pleural effusion remained almost stable after IFN gamma instillation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Since IFN gamma and tumor necrosis factor alpha ( TNF alpha ) have been reported to synergize in antitumor effects in a number of models , we decided to examine whether the combined treatment with recombinant mouse IL 12 and recombinant human TNF alpha would produce similar effects . ^^^ Potentiation of antitumor effects , which was observed in IL 12 / TNF alpha based immunotherapy , could result from at least three different mechanisms , partly related to stimulation of IFN gamma and TNF alpha production in treated mice : ( a ) direct cytostatic / cytotoxic effects on tumor cells , ( b ) induction of antitumor activity of macrophages , and ( c ) inhibition of blood vessel formation in the tumor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokines assessed included IL 1 alpha , IL 1 beta , IL 1ra , IL 6 , IL 8 , IL 12 , IL 13 , TNF alpha , TGF beta , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of L . monocytogenes infection on IL 4 producing liver NK T lymphocytes were reversed by antibody neutralization of IL 12 but not of IFN gamma or tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To examine this possibility , gamma interferon ( IFN gamma ) and / or tumor necrosis factor alpha ( TNF alpha ) were neutralized in BALB / c mice by intraperitoneal administration of monoclonal antibodies . ^^^ Neutralization of IFN gamma and / or TNF alpha resulted in increased splenic infection with wild type salmonellae after oral inoculation ; however , Spv salmonellae were defective at increasing splenic infection in cytokine depleted mice . ^^^ The use of a temperature sensitive marker plasmid , pHSG 422 , indicated that neutralization of IFN gamma caused less killing of wild type S . typhimurium , while neutralization of TNF alpha resulted in an increased in vivo replication rate for wild type salmonellae . ^^^ These results demonstrate that the Spv virulence function is not to evade suppression of bacterial infection normally mediated by IFN gamma or TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inflammatory cytokines such as interleukin 1 ( IL 1 ) , interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) induce iNOS expression in various cells including macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Out of phorbol 12 myristate 13 acetate ( PMA ) , cholera toxin , IL 1 beta , and tumor necrosis factor ( TNF ) alpha , the former three were as effective as IFN gamma in KG 1 cells , but only TNF alpha stimulated HGF / SF production in RPMI 8226 cells , whose effect was less than those of IFN alpha , IFN beta , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Secretion of RANTES protein in supernatants was investigated after stimulation with 50 ng / ml tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 beta ( IL 1 beta ) , lipopolysaccharide ( LPS ) , phorbolmyrisate acetate ( PMA ) , interferon gamma ( IFN gamma ) and serum free medium ( SFM ) for 24 hours . ^^^ Cultivated nasal , laryngeal and tracheal fibroblasts secreted RANTES protein upon TNF alpha , IL 1 beta and IFN gamma stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neutralization of IFN gamma and TNF alpha , but not IL 1 , IL 2 , or TGF beta , decreased production of virus by 4 logs and 2 logs , respectively . ^^^ Subsequently , addition of recombinant IFN gamma or TNF alpha to purified monocyte cultures was sufficient to produce HCMV permissive MDM . ^^^ While IFN gamma and TNF alpha possess antiviral properties , addition of these cytokines to permissive MDM cultures did not affect production of HCMV . ^^^ Thus , rather than inhibiting replication of HCMV , IFN gamma and TNF alpha specifically induce differentiation of monocytes into HCMV permissive MDM , which are resistant to the antiviral effects of these cytokines . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma , IL 1 beta , IL 4 , IL 6 , IL 10 , IL 12 , and TNF alpha mRNA levels were elevated at 12 hr postinoculation ( pi ) in spleen and CSF . ^^^ TGF beta was high in CSF at 48 hr , and some elevation of IL 1 beta , IL 6 , IL 10 , TNF alpha , IFN gamma , and IL 12 was evident at 72 hr pi . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Induction of nitric oxide synthase ( iNOS ) and production of the toxic metabolite nitric oxide ( NO ) is one of the interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) regulated effector mechanisms that can lead to apoptosis of haemopoietic progenitor cells . ^^^ Fas receptor ( Fas R ) expression can be stimulated by IFN gamma and TNF alpha . ^^^ Our data suggest that the inhibitory effects of Fas , including induction of apoptosis , are mediated by effector mechanisms that may be similar to those described for IFN gamma and TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Effect of tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) on human sperm motility , viability and motion parameters . ^^^ Previous studies have shown the presence of cytokines such as tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) in the semen of infertile men . ^^^ The present study was undertaken to investigate the in vitro effects of TNF alpha and IFN gamma on human sperm motion , viability and the hypoosmotic swelling test ( HOST ) . ^^^ Washed spermatozoa from normal volunteers ( n = 9 ) were incubated in the presence / absence of TNF alpha ( 1 microgram / mL ) plus IFN gamma ( 0 . 1 microgram / mL ) . ^^^ There was a time dependent negative effect of TNF alpha plus IFN gamma on sperm motility , viability , HOST , and lateral head displacement ( ALH ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Accordingly , in inflamed periodontal tissues , gingival fibroblasts and periodontal ligament fibroblasts stimulated with pro inflammatory cytokines such as IL 1 or TNF alpha , may produce IL 6 , and this production can be differentially modulated by endogenous PGE 2 , IL 6sR , T cell derived cytokines such as IFN gamma or IL 4 , and glucocorticoids . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines studied were interferon alpha ( IFN alpha ) , IFN gamma , interleukin 1B ( IL 1B ) , IL 12 , and tumor necrosis factor alpha ( TNF alpha ) . ^^^ IFN alpha , IFN gamma , IL 1 beta , IL 12 , and TNF alpha had limited effects on m RCA expression on uveal melanoma cells in vitro . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The aim of our study was to investigate , first , the phenotype of lymphocytes ( LC ) of PA patients using flow cytometry ( FC ) with regard to activation antigens and adhesion molecules ; second , the adhesion of LC of PA patients on cultivated resting or activated ( with thrombin , LPS , IFN gamma , or TNF alpha ) human umbilical vein endothelial cells ( HUVEC ) by counting the Feulgen stained nuclei of both adherent LC and HUVEC using image analysis ; and third , the synthesis of IL 6 and IL 8 in both LC and HUVEC 24 hr after cell contact . ^^^ It could be reduced with monoclonal antibodies ( MoAbs ) against CD11a and CD 18 on IFN gamma or TNF alpha activated HUVEC but was generally enhanced after treatment of HUVEC with MoAbs against CD 54 , CD62E , or CD 106 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunocytochemistry on serial paraffin sections was used to monitor the production dynamics of cytokines ( IL 2 , IL 4 , IL 6 , IL 10 , IFN gamma and TNF alpha ) and viral antigens in the trigeminal ganglion ( TG ) and the central side of the dorsal root entry zone ( DRE ) of mice , following infection of the cornea with herpes simplex virus type 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The secretion of IL 1 beta , IFN gamma , TNF alpha , IL 6 IL 10 and TGF beta by PBMCs was detected using ELISA methods . ^^^ The levels of IL 1 beta , IFN gamma and TNF alpha are significantly higher in the supernatants of the PBMCs which produced greater amounts of NO ( p < 0 . 02 , p < 0 . 03 , p < 0 . 01 , respectively ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OBJECTIVE : To test the hypothesis that interleukin 1beta ( IL 1beta ) and tumor necrosis factor alpha ( TNF alpha ) regulate the production of C 3 in the enterocyte at the transcriptional level and that this regulation is potentiated by interferon gamma ( IFN gamma ) . ^^^ METHODS : Cultured Caco 2 cells , a human intestinal epithelial cell line , were treated with various concentrations of human recombinant IL 1beta ( 0 . 005 1 . 25 ng / mL ) or TNF alpha ( 1 1000 U / mL ) with or without the addition of IFN gamma ( 250 U / mL ) . ^^^ The use of IFN gamma alone did not affect C 3 production but potentiated the effect of IL 1beta and TNF alpha in a synergistic manner . ^^^ CONCLUSIONS : C 3 production in the enterocyte is regulated by IL 1beta and TNF alpha at the transcriptional level , and this response is potentiated by IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although bone marrow derived Cr macrophage do not produce NO in response to LPS , both Sn and Cr macrophages release NO upon stimulation with IFN gamma and tumor necrosis factor , indicating that they are responsive to activation by these cytokines . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A local increase in IFN gamma gene expression increased a local expression of inducible nitric oxide synthase ( iNOS ) and the tumor necrosis factor a gene . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Circulating levels of interleukin 1 beta ( IL 1 beta ) , interleukin 6 ( IL 6 ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) were measured in 50 AAA patients ( 40 men , 10 women ) , 42 patients with coronary heart disease ( CHD ) ( 23 men , 19 women ) , and 38 controls whose angiogram was normal ( 17 men , 21 women ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
With combinations of TNF alpha and IFN gamma , ICAM 1 expression on HRV infected cells was reduced to basal levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
T cell and macrophage derived cytokines ( IL 2 , interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha , IL 1beta , IL 6 ) were released with different kinetics in the circulation of these mice . ^^^ The protective effects of SF were dose dependent and accompanied by profound modifications of blood levels of cytokines induced by Con A , so that , relative to control mice , SF ( 80 mg / kg ) treated animals showed markedly diminished plasma levels of IL 2 , IFN gamma and TNF alpha , along with augmented levels of IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both groups showed MHC class 1 restricted cytotoxicity against CD4+ T cells and were able to secrete IFN gamma , TNF alpha / beta and TGF beta . ^^^ Moreover , they were able to secrete IFN gamma and TNF alpha / beta , but not TGF beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our data indicate that the cytokines interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) inhibit transgene expression from certain widely used viral promoters / enhancers ( cytomegalovirus , Rous sarcoma virus , simian virus 40 , Moloney murine leukemia virus long terminal repeat ) delivered by adenoviral , retroviral or plasmid vectors in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , the inhibition of cell proliferation and suppression of natural cytotoxicity was associated with marked decrease in cytokine [ interleukin 1 ( IL 1 ) , IL 2 , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) ] production in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Potent antiviral activity was mediated by many of these encoded factors , including IL 2 , IL 12 , IFN gamma , TNF alpha , CD40L , Mig and Crg 2 . ^^^ In some cases , host defense mechanisms were induced ( IL 2 , IL 12 , Mig and Crg 2 ) , whilst for others , a direct antiviral effect was demonstrated ( IFN gamma , TNF alpha and CD40L ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Patients treated by ILP without TNF / interferon gamma ( IFN gamma ) had no detectable increase in either sTNF Rs or in circulating TNF , demonstrating that the release of TNF Rs was dependent upon the administration of TNF / IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although high levels of IFN gamma , tumor necrosis factor ( TNF ) alpha and interleukin ( IL ) 1 beta were released into the intestinal tracts of the parasitised mice at challenge infection , there was almost no release of these cytokines in mice treated with the MAb . ^^^ These results strongly suggest that IFN gamma is the most important ( or essential ) cytokine in protective immunity to H . nana re infection , rather than TNF alpha and IL 1 beta . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Graft infiltrating cells , deposition of cytokines ( interferon gamma [ IFN gamma ] and tumor necrosis factor alpha [ TNF alpha ] ) , IgM and C , and expression of endothelial cell ( EC ) P and E selectins were investigated by immunohistochemistry . ^^^ RESULTS : Xgs rejected after 3 days by LF treated rats showed an absence of IgM , C , and T cells , but the infiltration of NK cells and Mphi , together with the presence of IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Because IL 12 has previously been shown to modulate the expression of cytokines , including gamma interferon ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and IL 10 , which regulate L . pneumophila growth , immunomodulatory effects of endogenous IL 12 on intrapulmonary levels of these cytokines during replicative L . pneumophila lung infection were subsequently assessed . ^^^ Results of these experiments demonstrated that TNF alpha activity was significantly lower , while protein levels of IFN gamma and IL 10 in the lung were similar , in L . pneumophila infected mice administered IL 12 antiserum , compared to similarly infected untreated mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Compared to healthy controls , stimulated PBMC from HD patients produced similar amounts of T cell derived cytokines ( interleukin 2 ( IL 2 ) and interferon gamma ( IFN gamma ) ) , but greater amounts of monocyte derived inflammatory cytokines ( IL 1 beta , tumor necrosis factor alpha ( TNF alpha ) , and IL 8 ) and a regulatory cytokine ( IL 10 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) + / tumor necrosis factor alpha ( TNF alpha ) induces antiproliferation and intracellular Ca2+ store depletion in a human submandibular ductal cell line ( HSG ) , which can be reversed on cytokine removal [ A . ^^^ The intracellular Ca2+ pump [ sarco ( endo ) plasmic reticulum Ca ( 2+ ) ATPase ( SERCA ) ] protein in lysates and membranes of cells treated with IFN gamma + / TNF alpha , but not with TNF alpha alone , showed a similar time dependent decrease ( examined using a SERCA 2 antibody ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mutant mice deficient in major histocompatibility complex class 2 molecules ( A beta ( / ) ) , T cell receptor alphabeta cells ( TCR beta ( / ) ) , or all mature T and B lymphocytes ( RAG 1 ( / ) ) were naturally susceptible to P . carinii , whereas mouse mutants lacking the gamma interferon ( IFN gamma ) receptor ( IFN gamma R ( / ) ) or tumor necrosis factor alpha ( TNF alpha ) type 1 receptor ( p 55 ) ( TNF alpha RI ( / ) ) resisted disease acquisition . ^^^ Analysis of pulmonary cytokine patterns and free radical expression revealed the presence of superoxide , nitric oxide , and interleukin 1 ( IL 1 ) mRNA and elevated levels of IFN gamma , TNF alpha , and IL 12 in diseased TCR beta ( / ) and RAG 1 ( / ) mice . ^^^ Interestingly , neither IFN gamma nor TNF alpha appeared to be essential for resisting natural infection with P . carinii , nor were these cytokines sufficient for mediating resistance during established disease in the absence of CD4+ T lymphocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Synergic antitumor efficacy of TNF alpha , cytokines , such as interferon gamma ( IFN gamma ) and interleukin 2 ( IL 2 ) , and cytostatic drugs ( etoposide , cyclophosphamide ) was proved . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Therefore , we compared the transcription of the cytokine genes encoding IL 2 , IL 4 , IL 6 , IL 10 , IFN gamma , TNF alpha , and TGF beta 1 , and the Epstein Barr virus encoded BCRF 1 gene , in cells from five monkey and two human tumor specimens . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Elevated serum levels of several cytokines including interleukin ( IL ) 2 , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha were found . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The mean titer of antibody to EC treated with IFN gamma , IL 1 ( aIL 1 EC ) or TNF alpha ( aTNF EC ) was significantly higher than that of aEC in the sera of the MCTD patients with proteinuria , and the mean titer of aTNF EC was significantly higher than that of aEC in the sea of the MCTD patients with pulmonary fibrosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It was found that pentoxifylline ( Ptx ) was able to inhibit significantly the HLA DR expression and glycosaminoglycan synthesis induced by inflammatory cytokines including TNF alpha , IFN gamma and IL 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Oligonucleotide primers were designed to amplify porcine cytokine cDNA from genes encoding IL 1 alpha , IL 1 beta , IL 2 , IL 4 , IL 6 , IL 8 , IL 10 , IL 12 , IFN gamma , TNF alpha , TNF beta and the housekeeping genes beta actin and cyclophilin by PCR . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
LPS , interleukin 1 beta ( IL 1beta ) , interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) alone or in combinations induced the production of NO to different degrees . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cells were incubated for up to 72 h in the presence of IFN gamma , TNF alpha and IL 4 , and immunohistochemical staining was done with monoclonal antibodies with specificity for HLA class 1 heavy chain , HLA DQ ( T22 ) , HLA DR ( T36 ) and HLA DQ , R and P ( T35 ) . ^^^ Staining with T22 and T36 was always lower than with T35 , and additive or even synergistic results were obtained when cells were stimulated with a combination of IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We compared pleural fluid levels of ADA , TNF alpha , IFN gamma , IL 2 , IL 6 , IL 8 , pH , protein , glucose , cholesterol , triglyceride , amylase and lactic dehydrogenase between tuberculous and malignant effusions . ^^^ RESULTS : Compared to malignant effusion , tuberculous effusion showed significantly higher levels of ADA , IFN gamma , TNF alpha and IL 2 . ^^^ There was a good correlation between IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The multivariate statistical method supported the above suggestions and we also found that , in healthy subjects , the up and down regulation of sIL 2R in the serum within the physiological ranges seems to have a regulating role in the relationships between TNF alpha , IFN gamma and IL 4 , IL 6 , contributing to the operation of the cytokine network between TH 1 and TH 2 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Conditioned media and extracts from human RPE cells stimulated with various physiologic concentrations of interleukin 1 beta ( IL 1 beta ) ( 0 . 2 20 ng ml 1 ) , tumor necrosis factor ( TNF alpha ) ( 0 . 2 20 ng ml 1 ) or interferon gamma ( IFN gamma ) ( 10 1000 U ml 1 ) were examined to compare secreted and cell associated levels of IL 8 and MCP 1 at various time points up to 24 hr . ^^^ Dose and time dependent RPE cell MCP 1 secretion was also observed following IL 1 beta > TNF alpha > IFN gamma stimulation , with an average of 4 % of the total MCP 1 retained within RPE . ^^^ Using in situ hybridization IL 8 mRNA was readily detected within IL 1 beta > TNF alpha stimulated RPE cells and MCP 1 mRNA easily visualized within IL 1 beta > TNF alpha > or IFN gamma stimulated cells . ^^^ Immunohistochemical staining for MCP 1 in RPE cells was more intense and was visualized within RPE cells stimulated with IL beta , TNF alpha , or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The balance of stimulatory cytokines and inhibitory cytokines for NO production may play a critical role in the defense mechanism against M . tuberculosis considering that NO production is upregulated by IFN gamma , TNF alpha , and IL 1 beta and downregulated by IL 10 and TGF beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To clarify whether regulatory cytokines inhibit hematopoiesis in patients with myelodysplastic syndromes ( MDS ) , malignancies characterized by the formation of cytopenias despite the presence of cellular bone marrow , expression of TNF alpha and IFN gamma by bone marrow cells was investigated using specific reverse transcriptase polymerase chain reaction assays . ^^^ Immunohistochemical examination confirmed the scattered presence of TNF alpha or IFN gamma producing cells in the bone marrow of MDS patients . ^^^ These results suggested that disruption of hematopoiesis in MDS might be caused by enhanced production of inhibitory regulatory cytokines especially TNF alpha and occasionally IFN gamma by bone marrow macrophages . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this report , we describe a whole blood method for the determination of intracellular cytokines ( IFN gamma , TNF alpha and IL 2 ) that uses small volume ( 500 microl ) blood samples . ^^^ Kinetic studies showed maximal cytokine expression after 2 h of culture for TNF alpha and IFN gamma and 4 h for IL 2 . ^^^ Assessment of TNF alpha and IFN gamma production within the CD 4 and CD 8 lymphocytes from 10 normal volunteers showed that considerably more CD 8 + than CD 4 + cells produced IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha stimulated IL 8 mRNA expression and protein release in a time and dose dependent manner , whereas IFN gamma alone had no effect . ^^^ IL 8 release induced by TNF alpha and IFN gamma was partly inhibited by the Th 2 derived cytokines IL 4 , IL 10 , and IL 13 , as well as by dexamethasone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Production of IFN gamma by HIV+CD4 cells was markedly reduced , while IL 2nd tumor necrosis factor alpha ( TNF alpha ) production by HIV+CD4 remained relatively intact until the disease progressed further . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In order to study cytokine production profile ( IFN gamma , IL 4 and TNF alpha ) and TCRBV gene usage of peripheral autoreactive T cells from IDDM patients , we have generated antigen specific T cell lines with either tetanus toxoid , insulinoma membranes or a single beta cell protein , recombinant ICA 69 , which has been shown to be a target of both autoantibodies and T cells in IDDM . ^^^ T cell responses against beta cell antigens and tetanus toxoid ( TT ) were shown to be associated with IFN gamma and TNF alpha production , suggestive of a Th 1 like phenotype of the T cell lines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum samples were obtained from 11 male patients before and at intervals for up to 10 days after surgery , and were assayed for total cholesterol ( TC ) , triglycerides ( TG ) , high density lipoprotein cholesterol ( HDL C ) , cortisol , tumor necrosis factor alpha ( TNF alpha ) , interleukin 1beta ( IL 1beta ) , interleukin 6 ( IL 6 ) , and interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study investigates ( 1 ) whether ET 1 stimulates the formation of tumour necrosis factor alpha ( TNF alpha ) or interferon gamma ( IFN gamma ) in cultured macrophages or in the anaesthetized rat . ^^^ Incubation of J774 . 2 macrophages with ET 1 ( 0 . 001 1 microM ) caused a concentration and time dependent increase in the concentration of TNF alpha , but not of IFN gamma , in the culture medium . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : ELIspot assays were used to detect mononuclear cells in the peripheral blood ( PMBC ) and synovial fluid ( SFMC ) secretion the type 1 cytokines interleukin 2 ( IL 2 ) and interferon gamma ( IFN gamma ) , the type 2 cytokines IL 4 , IL 6 and IL 10 , and the inflammatory cytokine tumor necrosis factor alpha ( TNF alpha ) . ^^^ CONCLUSION : Patients with new onset synovitis have increased numbers of PBMC secreting IL 2 and IFN gamma , while patients with chronic RA have increased numbers of PBMC secreting IL 6 , IL 10 , and TNF alpha . ^^^ Correlations between joint score and number of PBMC secreting cytokines suggest the number of PBMC secreting IFN gamma is most relevant in new onset synovitis , while the number of PBMC secreting IL 6 , IL 10 , and TNF alpha is of greater relevance in chronic RA . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The half life of TNF R 75 mRNA in cells exposed to GM CSF + IFN gamma did not differ significantly from that in untreated cells . ^^^ According to nuclear run on assays the synthesis of TNF R 75 mRNA in cells treated with GM CSF + IFN gamma , as well as with the phorbol ester TPA , was markedly increased compared to untreated cells , indicating that the observed changes in receptor expression primarily involve altered transcription of the gene . ^^^ The results suggest that in inflammatory processes , GM CSF and IFN gamma contribute to increased synthesis of TNF R 75 by monocytic cells , a prerequisite for the formation of large amounts of soluble receptors . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , in RA patients both a decrease in IFN gamma and an increase in IL 4 production correlated with an increase in serum TNF alpha , ESR , CRP , and joint destruction . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Substantial amounts of nitrite ( a degradation product of NO ) were produced and the initial rickettsial infection was suppressed in cultures of L 929 cells treated with crude lymphokine preparations ( LK ) or with gamma interferon ( IFN gamma ) plus tumor necrosis factor alpha ( TNF alpha ) but not in L 929 cell cultures treated with IFN gamma alone or TNF alpha alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin 1 receptor antagonist ( IL 1RA ) , soluble IL 2R ( sIL 2R ) ( sCD 25 ) , IL 6 , IL 8 , tumor necrosis factor alpha ( TNF alpha ) , soluble TNF receptor type 2 ( sTNF RII ) ( sCD120b ) , gamma interferon ( IFN gamma ) , soluble intercellular adhesion molecule 1 ( sICAM 1 ) ( sCD 54 ) , soluble E selectin ( sE selectin ) ( ELAM 1 ; sCD62E ) , sCD 14 , and neopterin were measured with commercial test kits . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Expression of beta actin , IL 1 alpha , IL 1 beta , IL 6 , IL 10 , tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) and transforming growth factor beta 1 ( TGF beta 1 ) mRNA was analysed in colon and small intestinal tissue of both IL 2 deficient ( IL 2 / ) mice and normal ( wild type ) litter mates ( IL 2+ / + ) at different ages by using qualitative , as well as semiquantitative , competitive reverse transcription polymerase chain reaction ( RT PCR ) . ^^^ RESULTS : IL 2 / mice had expressed low levels of IL 1 alpha , IL 1 beta , IL 6 , TNF alpha , and IFN gamma mRNA in the colon by 1 . 5 weeks of age . ^^^ In advance of the development of histologically and clinically detectable bowel inflammation , expression of IL 1 alpha , IL 1 beta , IL 6 , TNF alpha , IFN gamma , and IL 10 , but not TGF beta 1 , mRNA increased in the colon of IL 2 deficient mice . ^^^ In contrast , IL 2+ / + mice expressed TGF beta 1 mRNA in colon tissue at 13 and 23 weeks of age , but not IL 1 alpha , IL 1 beta , IL 6 , TNF alpha , IL 10 , or IFN gamma mRNA . ^^^ CONCLUSIONS : Bowel inflammation in IL 2 deficient mice is preceded by an increase in IL 1 alpha , IL 1 beta , TNF alpha , and IFN gamma mRNA expression in colon tissue . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The production of Interferon gamma ( IFN gamma ) and Tumor Necrosis Factor ( TNF alpha ) by mitogen activated CD2+ lymphocytes from B CLL patients was higher than that found in healthy controls , while no differences were found in TNF beta production . ^^^ IFN gamma and TNF alpha levels determined at 72 h in PHA stimulated CD2+ cell cultures from B CLL patients statistically correlated with the percentages of CD3+CD45RO+ and CD 3 CD56+ lymphocytes , respectively . ^^^ The production of both IFN gamma and IL 4 by PHA stimulated CD2+ lymphocytes from non smouldering B CLL patients was significantly higher than that from smouldering B CLL patients while no significant differences were found in the production of IL 2 , TNF alpha , and TNF beta between the two B CLL patient groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pretreatment with either anti tumor necrosis factor alfa ( TNF alpha ) or anti interferon gamma ( IFN gamma ) monoclonal antibody ( MAb ) moderately decreased hemostasis and hepatic injury , whereas combined use of two MAbs almost perfectly protected mice from these disorders . ^^^ Complete obliteration of hemostasis and hepatic injury was also accomplished by the pretreatment with FK 506 which suppressed TNF alpha and IFN gamma production . ^^^ These findings indicate that the agglutination activities of Con A and T cell activation mediated TNF alpha / IFN gamma production are both required for the induction of intrasinusoidal hemostasis , which is indispensable for the development of hepatic injury . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Normal C57BL / 6 and BALB / c spleen cells produced comparable levels of T cell derived lymphokines ( interferon gamma [ IFN gamma ] , tumor necrosis factor alpha [ TNF alpha ] , and interleukin 2 [ IL 2 ] ) following in vitro stimulation with Con A . ^^^ A single intravenous injection of Con A to C57BL / 6 mice induced the plasma levels of TNF alpha and IL 2 comparable with or slightly higher than those observed in BALB / c mice , whereas the same treatment resulted in an apparently lower level of IFN gamma production in C57BL / 6 mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
While MCM conditions only induced IFN gamma ( low ) , IL 4 ( neg ) cells , TNF alpha / IL 1 / IL 6 promoted growth of IFN gamma ( intermediate ) , IL 4 ( neg ) CD8+ T cells . ^^^ TNF alpha / IL 1 , IL 6 + PGE 2 cultured DC seem to be optimal for generation of IFN gamma producing CD4 / CD8+ T cells . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
High sensitivity of transgenic mice expressing soluble TNFR 1 fusion protein to mycobacterial infections : synergistic action of TNF and IFN gamma in the differentiation of protective granulomas . ^^^ To explore the respective roles of TNF and interferon ( IFN ) gamma in resistance to BCG and granuloma differentiation , controls and sTNFR 1 transgenic mice were compared to IFN gammaR mutant mice and mice double defective in TNF and IFN gamma activity ( obtained by crossing transgenic and mutant mice ) . ^^^ The three groups of deficient mice showed a strongly enhanced susceptibility to BCG infection , with the following decreasing order of sensitivity between groups : TNF + IFN gamma > TNF > IFN gamma deficient mice . ^^^ These studies show the essential role of both TNF and IFN gamma in the development , during mycobacterial infections , of protective granulomas containing highly differentiated macrophages capable of destroying ingested bacteria , and emphasize that these two cytokines act synergistically in granuloma formation . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Second , the amount of ERV 3 mRNA in cultured endothelial cells from human umbilical vein was significantly increased with any of TNF alpha , IL 1 beta or IL 1 alpha stimulation but decreased with IFN gamma treatment , by a quantitative reverse transcriptase polymerase chain reaction ( RT PCR ) with competitive PCR . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The results of the experiments from the mouse model of CMV IP suggested that the cytokines , such as IFN gamma and TNF alpha , and the cytokine induced nitric oxide mediate CMV IP . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We postulate that the functionally deficient NK cells fail to generate adequate amounts of IFN gamma ( and perhaps , TNF alpha ) to optimally activate macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , human M phi , stimulated with IFN gamma and LPs , produced TNF alpha suggesting that the lack of increment in NO 2 production was not due to an absence of response of M phi to the stimuli used . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Semiquantitative reverse transcriptase PCR analysis revealed that both DC subpopulations spontaneously expressed IL 1alpha , IL 1beta , IL 6 , IL 7 , IL 12 ( p 35 and p 40 ) , IL 15 , IL 18 , TNF alpha , TGF beta , macrophage CSF , and granulocyte macrophage CSF , but not IL 2 , IL 3 , IL 4 , IL 5 , IL 9 , and IFN gamma transcripts . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1 alpha and TNF alpha are required for IL 12 induced development of Th 1 cells producing high levels of IFN gamma in BALB / c but not C57BL / 6 mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma , TNF alpha , and inducible nitric oxide synthase were all expressed throughout the latent phase of infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , the results showed that the responsiveness of macrophages toward the TNF alpha inducing activity of VSG was strictly dependent on the activation state of the macrophages , since resident macrophages required IFN gamma preactivation to become responsive . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
High levels of TNF , soluble TNF receptors , soluble ICAM 1 , and IFN gamma , but low levels of IL 5 , are associated with hepatosplenic disease in human schistosomiasis mansoni . ^^^ In a case control study based in two areas of Kenya , hepatosplenic schistosomiasis mansoni was shown to be linked with low levels of IL 5 and with correspondingly high IFN gamma , TNF , and circulating soluble TNF receptor 1 ( sTNFR 1 ) , sTNFR 2 , and sICAM 1 . ^^^ PBMC from the hepatosplenic cases responded to in vitro Ag stimulation with significantly higher levels of IFN gamma and TNF , but lower levels of IL 5 , compared with nonhepatosplenic controls matched for age and infection intensity . ^^^ However , principle component analysis identified a high IFN gamma and TNF , and low IL 5 axis in the data as the first principle component ; this was significantly associated with hepatosplenomegaly ( p < 0 . 0005 ) even after controlling for area . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Molecular cloning of the woodchuck cytokines : TNF alpha , IFN gamma , and IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have measured by flow cytometry the ability of subsets of CD8+CD3+ lymphocytes within mononuclear cell preparations to make intracellular cytokines ( IL 2 , tumour necrosis factor alpha ( TNF alpha ) and IFN gamma ) on stimulation in vitro with phorbol myristate acetate ( PMA ) and ionomycin for 16 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increased fractions of interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) and IL 2 positive CD4+ T cells were found in the spleens of diseased mice compared with control mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Apoptosis of colorectal adenocarcinoma ( COLO 201 ) by tumour necrosis factor alpha ( TNF alpha ) and / or interferon gamma ( IFN gamma ) , resulting from down modulation of Bcl 2 expression . ^^^ However , TNF alpha and / or IFN gamma , independently and additively , up regulated Fas antigen expression on COLO 201 and induced apoptosis in a dose dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In A / J mice , splenocytes express IL 1alpha , IL 10 , and IFN gamma mRNA in vivo and secrete high levels of TNF alpha in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The data base included measurements of interleukin 1 ( IL 2 , IL 4 , IL 6 , IL 10 , IL 12 , soluble intercellular adhesion molecule 1 ( sICAM 1 ) , tumour necrosis factor alpha ( TNF alpha ) , soluble CD 14 ( sCD 14 ) , interferon gamma ( IFN gamma ) , GM CSF , volume of urine and its pH . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Since cytokines are involved in this auto immune beta cell damage , we used an ELISPOT assay to enumerate the islet associated T cells that secreted interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) or interleukin 4 ( IL 4 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokines and receptors reportedly affected by IFN alpha include interleukin 1 ( IL 1 ) , IL 2 , IL 6 , IL 8 , IL 1 receptor , IL 1 receptor antagonist , tumor necrosis factor , tumor necrosis factor receptor , and IFN gamma , all of which may amplify the effects of IFN alpha treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , TP 72 suppressed NF KB activation in primary macrophages treated with the combination of IFN gamma and LPS or IFN gamma and tumor necrosis factor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OBJECTIVE : To determine the effects of interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) , alone or in combination , on the expression of aggrecan , biglycan , and decorin core protein genes in human chondrocytes . ^^^ METHODS : Isolated human chondrocytes were cultured on poly ( 2 hydroxyethyl methacrylate ) coated plastic dishes to prevent the loss of cartilage specific phenotype , and the effects of IFN gamma and TNF alpha , alone or in combination , on aggrecan , biglycan , and decorin core protein gene transcription and steady state messenger RNA ( mRNA ) levels were examined . ^^^ RESULTS : The addition of IFN gamma ( 1 . 5 pM ) or TNF alpha ( 0 . 3 pM ) caused a decrease in the steady state level of aggrecan mRNA ( 25 % and 15 % , respectively ) , and the combination of these low concentration cytokines caused a potent inhibition ( 66 % ) . ^^^ The combination of IFN gamma and TNF alpha resulted in a potentiation of the inhibitory effects of TNF alpha on biglycan mRNA levels ( 79 % ) and transcription rate of the biglycan core protein gene ( 46 % ) . ^^^ Notably , the combination of IFN gamma and TNF alpha potentiated the inhibitory effects of IFN gamma on decorin mRNA ( 80 % ) and on the transcription of the corresponding gene ( 43 % ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have shown that PTX affects cytokine ( interferon gamma , IFN gamma ; interleukin 1 , IL 1 ; tumour necrosis factor alpha , TNF alpha ) induced NO production in both cell types , but in the opposite manner enhancing in astrocytes and suppressive in macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This regime was associated with reduced IFN gamma levels produced by ex vivo CII stimulated draining lymph node cells , and with diminished spontaneous ex vivo production of tumour necrosis factor ( TNF ) , IL 6 and IL 10 by freshly isolated synovial cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) , IL 2 , IL 6 , IL 10 and tumour necrosis factor alpha ( TNF alpha ) production in culture supernatants were determined by cytokine specific ELISAs . ^^^ Compared with controls , PHA stimulated cultures resulted in significantly higher IL 10 ( P < 0 . 001 ) , IL 2 ( P < 0 . 005 ) and IFN gamma ( P < 0 . 001 ) levels in IgAN patients , but no significant differences in TNF alpha or IL 6 levels were found . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Following exercise , significant decreases in LPS induced release of IL 6 , tumour necrosis factor alpha ( TNF alpha ) and IL 10 and anti CD2 / anti CD 28 MoAb stimulated secretion of interferon gamma ( IFN gamma ) were observed . ^^^ In addition , the inhibitory effect of dexamethasone on both IL 6 and TNF alpha secretion was significantly reduced following exercise , whereas that on IL 10 and IFN gamma release was not affected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When mice were treated with monoclonal antibodies ( MAbs ) against tumor necrosis factor alpha ( TNF alpha ) or gamma interferon ( IFN gamma ) before S . aureus infection , the induction of iNOS mRNA expression in the kidneys was inhibited . ^^^ Production of TNF alpha and IFN gamma was not affected by AG treatment in vitro and in vivo . ^^^ Moreover , our results show that TNF alpha and IFN gamma regulate NO production while NO may not be involved in the regulation of the production of these cytokines during S . aureus infection . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Despite this defect , both mutant mouse strains had a rather normal proinflammatory cytokine response ( interleukin 12 [ IL 12 ] , IFN gamma , IL 6 ) , with the exception of an impaired tumor necrosis factor alpha and IL 1alpha response in IFN gammaR ( / ) mice , demonstrating that only the latter two cytokines are dependent on IFN gamma activation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Proliferation induced by PMA was enhanced 2 20 fold when the cells were co cultured with either anti Ig , IL 2 , IL 4 , IL 13 , IFN gamma or TNF alpha . ^^^ When taken together with the finding that IL 2 , IL 4 , IL 13 , IFN gamma and TNF alpha prevent in vitro apoptosis of leukaemic CD5+ B cells , the results presented here suggest that these cytokines , in conjunction with signals delivered via sIg , may play a role in the proliferation of leukaemic B cells in vivo and , consequently , the pathogenesis of B CLL . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MF also decreased production of tumor necrosis factor alpha ( TNF alpha ) by macrophages stimulated with lipopolysaccharide and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In mice , in vivo , cytokine inducing activities such as IL 1 , IFN gamma , TNF alpha , GM CSF and chemotactic factor were observed and in vitro , TNF alpha , GM CSF , chemotactic factor , complement , NO , PGE 2 inductions and protein kinase C activation were demonstrated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After injection of 100 350 micrograms LPS into mice , cytokines including TNF alpha , IL 6 , IL 10 , IL 1 beta , GM CSF and IFN gamma were measured in the serum . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Phagocyte recruitment was almost totally ablated by neutralization of either tumour necrosis factor alpha ( TNF alpha ) or interferon gamma ( IFN gamma ) in vivo demonstrating that granuloma formation was dependent on the presence of these cytokines . ^^^ This was concomitant with a reduction in the in situ cytokine mRNA levels otherwise induced in infected mice , for chemokines , pro inflammatory and regulatory cytokines , including TNF alpha , IFN gamma , macrophage inflammatory protein 1 alpha , interleukin 1 beta ( IL 1 beta ) and IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Messenger RNAs ( mRNAs ) for HSPs ( HSP 32 , HSP 47 , HSP 60 , HSP 70 , HSP 84 and HSP 86 ) and cytokines [ interleukin 1 beta ( IL 1 beta ) , interleukin 6 ( IL 6 ) , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) ] were detected by reverse transcription polymerase chain reaction ( RT PCR ) . ^^^ Furthermore , the expression of IL 1 beta , IL 6 , TNF alpha and IFN gamma mRNA was associated with expression of HSP 60 , HSP 47 , HSP 70 and HSP70 / HSP86 mRNA , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , other cytokines such as IFN gamma , IL 12 and IL 18 are also produced and affect the production and cellular response to IL 1 and TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These actions of IFN gamma were not mediated by TNF alpha production or Fas Fas ligand interactions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Type 1 and type 2 like lesional skin derived Mycobacterium leprae responsive T cell clones are characterized by coexpression of IFN gamma / TNF alpha and IL 4 / IL 5 / IL 13 , respectively . ^^^ To explore whether other cytokines are coproduced along with IFN gamma and IL 4 , we investigated the secretion of a panel of other cytokines ( TNF alpha , IL 5 , IL 6 , IL 10 , and IL 13 ) by a large number of these TCC . ^^^ Upon analysis of 139 M . leprae responsive TCC , we observed a positive correlation in the coproduction of IFN gamma / TNF alpha ( r = 0 . 81 ) , and in that of IL 4 / IL 5 ( r = 0 . 83 ) , IL 4 / IL 13 ( r = 0 . 80 ) , and IL 5 / IL 13 ( r = 0 . 82 ) . ^^^ Polarized type 1 like TCC produced dominantly IFN gamma / TNF alpha , and polarized type 2 like TCC predominantly IL 4 / IL 5 / IL 13 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha had no effect on replication of H37Rv within MN , and IFN gamma mediated only a 1 . 9 fold reduction in bacterial growth . ^^^ Supernatants of cocultured H37Rv infected MN and NAC only partially reduced intracellular growth of M . tb despite containing nanogram concentrations of TNF alpha and IFN gamma . ^^^ Neutralizing antibodies to TNF alpha , IFN gamma , and IL 12 failed to affect the NAC mediated growth limitation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Malaria elicits type 1 cytokines in the human placenta : IFN gamma and TNF alpha associated with pregnancy outcomes . ^^^ Among primigravidas , placental TNF alpha concentrations were significantly higher in the presence of severe maternal anemia , and both IFN gamma and TNF alpha were significantly elevated when a low birth weight , rather than normal weight , infant was delivered . ^^^ We conclude that maternal malaria decreases IL 10 concentrations and elicits IFN gamma , IL 2 , and TNF alpha in the placenta , shifting the balance toward type 1 cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro effects of docetaxel on the human colon carcinoma cell line HT 29 were studied with respect to the expression of different adhesion and surface marker molecules , the adhesion and immunocytotoxicity of peripheral blood lymphocytes and the secretion of IFN gamma and TNF alpha . ^^^ Unstimulated and IL 2 stimulated lymphocytes secreted more TNF alpha and IFN gamma when cocultured with docetaxel treated HT 29 cells than with untreated cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A 60 protects mice against TB and cancer by inducing the synthesis of three lymphokines : interleukin 2 ( IL 2 ) , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The extracellular 5 HIAA levels were not changed in the NRD after peripheral application of rat recombinant IFN gamma , but elevated by the cytokines IL 1 beta and TNF alpha . ^^^ After intracerebroventricular ( i . c . v . ) application the cytokines IFN gamma , IL 1 beta and TNF alpha stimulated the serotoninergic transmission in the NRD . ^^^ In this respect the T cell and NK cell cytokine IFN gamma acts clearly different when compared to the mainly macrophage derived cytokines IL 1 beta and TNF alpha , and plays a different role in the communication between immune and central nervous system . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The IL 1 beta , IL 6 , TNF alpha , IL 8 , and IFN gamma levels in temporomandibular joint synovial fluid were measured using enzyme linked immunosorbent assay . ^^^ RESULTS : Measurable level of at least one cytokine in the synovial fluid was found in 40 ( 64 . 5 % ) of 62 joints in the patients : IL 1 beta and IFN gamma were each detected in 18 ( 29 . 0 % ) of 62 joints ; IL 6 in 13 ( 21 . 0 % ) of 62 joints ; IL 8 in 11 ( 19 . 3 % ) of 57 joints ; and TNF alpha in only 5 ( 8 . 1 % ) of 62 joints . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Additionally , the xenograft response , unlike the allograft response , was marked by an early increased expression of TNF alpha and IL S ( day 3 ) and an almost complete absence of IFN gamma expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
By contrast , co exposure of cells to LIF and IFN gamma , IL 6 and LIF , and INF gamma and TNF alpha resulted in a more than additive , synergistic , suppression of LPL activity with the maximum reduction and maximum degree of synergism produced by combinations of IFN gamma and TNF alpha . ^^^ The synergism between IFN gamma and TNF alpha was observed over a range of complementary dose combinations and also occurred when the cells were exposed first to INF gamma ( priming ) , washed , and then stimulated subsequently with TNF alpha . ^^^ The reduction in LPL activity by combinations of IFN gamma and TNF alpha and the priming action of IFN gamma were accompanied by a comparable decrease in LPL mRNA concentrations , thereby indicating that the major control responsible for the changes in LPL activity was being exerted at the level of mRNA metabolism ( decreased transcription or RNA stability ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1 beta , and to a lesser extent , TNF alpha and IL 4 increased NEP activity and expression , whereas IFN gamma decreased NEP . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here , we report that in patients with MS the combined action of interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNFalpha ) , interleukin ( IL ) 2 , and IL 6 leads to the activation of most peripheral T cells ( mainly CD 4 memory ) by promoting a persistent intracellular calcium increase via two independent signaling pathways . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Previously it has been demonstrated that human proximal tubular epithelial cells ( PTEC ) are able to produce chemokines ( such as IL 8 and MCP 1 ) and complement components ( such as C 2 , C 3 , C 4 and factor H ) , and that production of these proteins is regulated by pro inflammatory cytokines such as interleukin 1 alpha ( IL 1alpha ) , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PD 98059 inhibited TNF alpha , IL 3 , granulocyte macrophage ( GM ) CSF , IFN gamma , and to a lesser extent IL 6 and IL 10 production but enhanced IL 4 , IL 5 , and IL 13 production induced by CD3 / PMA or CD3 / CD28 . ^^^ FK 506 also reduced CD3 / CD28 induced production of IL 3 , IL 4 , IL 10 , TNF alpha , and IL 6 but augmented that of GM CSF , IL 5 , IFN gamma , and IL 13 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human NK cell activity can be augmented in vitro by stimulation with IL 2 or IL 12 , both of which also induce the production of IFN gamma , TNF alpha , and granulocyte macrophage CSF by NK cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Strong proliferation of resting human B cells induced in vitro by a variety of physiologic signals , including anti mu , CD 40 ligand , IL 2 , IL 4 , IL 6 , IL 13 , IFN gamma , TNF alpha , anti CD 19 , and anti CD 20 , failed to induce A myb expression , suggesting that proliferation alone is not sufficient for A myb expression in the absence of induction of a true centroblast phenotype . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The role of endogenous IFN gamma , TNF alpha and IL 10 in LPS induced nitric oxide release in a mouse model . ^^^ Mice injected with lipopolysaccharide ( LPS ) develop lethal septic shock , accompanied by elevated serum NOx , interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) and TNF receptor levels . ^^^ In vitro data indicate that IFN gamma and TNF alpha play an important role in LPS induced NO release . ^^^ Further , interleukin 10 ( IL 10 ) has been shown to inhibit the release of pro inflammatory cytokines such as IFN gamma and TNF alpha . ^^^ Therefore , in the present study , we investigated the role of IFN gamma , TNF alpha , and IL 10 in LPS induced NO release . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These enhanced host defenses were not reversed with pretreatment with a polyclonal anti TNF alpha antibody , suggesting that IFN gamma ' s effect was through a non TNF alpha dependent mechanism . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum interleukins ( IL ) and interferons ( IFN ) measured by EIA were as follows : IL 2 , 110 pg / ml ; IL 6 , 300 pg / ml ; IL 8 , 7 , 000 pg / ml ; TNF alpha , 35 pg / ml , IL 1 and IL 4 undetectable , IFN alpha 2 , 200 pg / ml , and IFN gamma 700 pg / ml . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In situ hybridization with cytokine probes detected a strong increase in the proportion of leukocytes positive for IFN gamma , tumor necrosis factor alpha , IL 1beta , and IFN inducible protein 10 at the site of rejection of IL 12 engineered tumor cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Role of IFN gamma , TNF alpha , IL 4 and IL 10 in the regulation of experimental Toxoplasma gondii infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha had no effect alone but enhanced class 2 expression in combination with IFN gamma , most notably for DQ and DP . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Add back of recombinant TNF alpha in combination with IFN gamma to normoxic epithelia recapitulated hypoxia and identified basolaterally polarized TNF alpha receptor types 1 and 2 on intestinal epithelia . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Previously we reported the induction of tumor necrosis factor ( TNF ) alpha mRNA in the liver of interferon ( IFN ) gamma transgenic mouse , which might be the result of IFN gamma gene expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The immunoreactivity was evaluated by assessing the induction of cytokine production ( IL 1 alpha , IL 1 beta , TNF alpha , IL 6 , IL 8 , IL 2 , IFN gamma , IL 4 , TNF beta and IL 10 ) , which was detected at the single cell level . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After reverse transcription a PCR method was applied to detect beta actin and cytokine mRNA expression ( interleukin ( IL ) 2 , IL 4 , IL 10 , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) ) . ^^^ The signal generated was directly proportional to the starting copy number of target molecules in the sample over 6 log concentrations and quantitative analysis of cDNA concentrations was performed in comparison to beta actin or cytokine cDNA standards . mRNAs coding for beta actin and TNF alpha were readily detectable in cDNAs prepared from the whole blood of eight healthy donors , while the other cytokines were expressed in lower amounts ( IFN gamma , IL 10 ) or were undetectable ( IL 2 , IL 4 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here we studied in mice the effects of rolipram , a selective PDE 4 inhibitor , and amrinone , a selective PDE 3 blocker , on plasma levels of IL 12 ( p 70 ) , IFN gamma , IL 1 , TNF alpha , and nitric oxide ( NO ) induced by intraperitoneal injection of Escherichia coli lipopolysaccharide ( LPS ) ( 80 mg / kg ) . ^^^ In addition , rolipram inhibited IL 12 , IFN gamma , TNF alpha and nitrite / nitrate ( breakdown products of NO ) production in C57BL / 6 IL 10 ( + / + ) mice as well as in their IL 10 deficient counterparts ( C57BL / 6 IL 10 ( / ) ) . ^^^ Our results suggest that rolipram and amrinone decrease the immune activation in endotoxemia through inhibition of the production of pro inflammatory mediators IL 12 , IFN gamma , TNF alpha and NO . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present report , we show that interferon gamma ( IFN gamma ) inhibits the expression of the PTX 3 gene induced by exposure to IL 1 , TNF or lipopolysaccharide in human monocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 12 neutralization both early and late in infection caused delayed resolution of parasite load , a transient decrease in IFN gamma , IL 4 , TNF alpha and inducible nitric oxide synthase ( NOS 2 ) production , and suppressed tissue granuloma formation in the liver of genetically susceptible BALB / c mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Small bowel enteropathy : role of intraepithelial lymphocytes and of cytokines ( IL 12 , IFN gamma , TNF ) in the induction of epithelial cell death and renewal . ^^^ The small bowel mucosa contains within its villus epithelium a large number of intraepithelial lymphocytes ( IEL ) which upon activation are cytotoxic and release large quantities of IFN gamma and TNF ; these activities are increased by in vitro exposure to IL 12 . ^^^ We propose that , when activated by their cognate ligands and / or IL 12 produced by cells in the lamina propria , IEL eliminate infected and senescent epithelial cells through a combination of cytotoxicity and of IFN gamma and TNF release . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
By reverse transcriptase based polymerase chain reaction , higher levels of TGF beta mRNA , and lower levels of TNF alpha and IFN gamma mRNA were found in the central nervous system ( CNS ) tissue of LR and co immunized mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In HSV 2 infected brains , ( 1 ) IL 1beta , TNF alpha and IFN gamma mRNA expression was low , ( 2 ) ICAM 1 and VCAM 1 were not induced , ( 3 ) few CD4+ or CD8+ cells were detected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Synergistic effect of IL 1alpha , IFN gamma , and TNF alpha on RANTES production by human renal tubular epithelial cells in vitro . ^^^ This study analyzes the production of RANTES in vitro by proximal tubular epithelial cells ( PTEC ) after stimulation with the inflammatory cytokines interleukin 1alpha , ( IL 1alpha ) , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) . ^^^ Unstimulated PTEC or PTEC stimulated with the cytokines IL 1alpha , IFN gamma , and TNF alpha alone did not produce detectable amounts of RANTES . ^^^ However , a combination of IFN gamma and either IL 1alpha or TNF alpha resulted in strong induction of RANTES production up to 2046 + / 817 pg / ml or 2595 + / 525 pg / ml per 1 10 10 ( 5 ) PTEC , respectively . ^^^ After stimulation with IL 1alpha and TNF alpha , RANTES production was less prominent than the combination of IFN gamma with either IL 1alpha or TNF alpha , and only detectable in 5 of 7 PTEC lines tested . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Numbers of AChR reactive IFN gamma and tumour necrosis factor alpha ( TNF alpha ) mRNA expressing lymph node cells from rats treated nasally with 600 microg / rat of AChR were suppressed , while IL 4 , IL 10 and transforming growth factor beta ( TGF beta ) mRNA expressing cells were not affected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines such as TNF alpha and interferon gamma ( IFN gamma ) are important for the elimination of infected hepatocytes during acute hepatitis B virus ( HBV ) infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of iNOS , interleukin 6 , and tumor necrosis factor alpha ( TNF alpha ) mRNAs was induced in both c Brucella and ops Brucella infected cells and was strongly potentiated by IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Analyses of plasma cytokines revealed peaks of tumor necrosis factor ( TNF ) alpha and interleukin 12 ( IL 12 ) 1 and 3 to 5 h after injection , respectively , and gradually rising levels of gamma interferon ( IFN gamma ) continuing until death . ^^^ Depletion of IL 12 , TNF , IFN gamma , and NO rescued mice from the lethal effect of antigen ( Ag ) and D Gal . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The results demonstrate that LPS , IL 1beta , and TNF alpha induce p 100 expression at mRNA and protein level while IFN gamma , IL 3 and IL 4 / IL 10 have no effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A significant decrease in the number of IL 2 or TNF alpha producing T cells was observed , whereas those producing IFN gamma remained preserved . ^^^ Furthermore , there is a gradient of susceptibility to activation induced apoptosis ( IL 2 < IFN gamma < TNF alpha ) among the different Th 1 subsets . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Reverse transcribed PCR showed that in contrast to TNFR 2 ( 0 / 0 ) and wt mice , TNFR 1 ( 0 / 0 ) mice were unable to up regulate inducible nitric oxide synthase mRNA transcripts in the course of infection , whereas intracerebral levels of IFN gamma , TNF alpha , and IL 1beta mRNA transcripts , recruitment of immune cells to the brain , and the amount of apoptotic cells in inflammatory foci did not differ significantly among the various experimental groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These data suggest that T cells from WG patients overproduce IFN gamma and TNF alpha , probably due to dysregulated IL 12 secretion , and that IL 10 may therefore have therapeutic implications for this disease . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PURPOSE : Hyperthermic isolated limb perfusion ( HILP ) with tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and melphalan is associated with a dramatic antitumor effect in locally advanced extremity soft tissue sarcomas ( STS ) . ^^^ The aim of this study was to demonstrate the feasibility and efficacy of adjuvant radiotherapy after HILP with TNF alpha , IFN gamma , and melphalan and delayed surgical resection . ^^^ CONCLUSION : Adjuvant EBRT after HILP with TNF alpha , IFN gamma , and melphalan and delayed tumor resection of locally advanced extremity STS is feasible and may increase local tumor control without increasing treatment morbidity . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To investigate the involvement of cytokines in MS , the mRNA levels of interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 4 ( IL 4 ) and interleukin 10 ( IL 10 ) in peripheral blood mononuclear cells without stimulation in vitro were quantified by a competitive reverse transcription polymerase chain reaction technique . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The acute stage of Trypanosoma cruzi infections related to high parasitemia is characterized by the presence of inflammatory infiltrates in several tissues , including the heart and squeletal muscle , as well as by an increased production of inflammatory mediators , such as gamma interferon ( IFN gamma ) , tumor necrosis factor ( TNF ) , interleukin 1 ( IL 1 ) and oxygen and nitrogen reactive intermediates . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Many of the cytokine mRNAs we investigated were steadily detected by RT PCR in efferent lymph cells of control sheep , but frequencies of detection of IL 2 , IFN gamma , IL 1 beta and TNF alpha mRNAs were augmented in efferent lymph cells following inoculation of both SAO virulent or vaccinal strains . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma and tumor necrosis factor alpha were also not detected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These reports led us to hypothesis that psoriatic skin could provide epidermal keratinocytes with TNF alpha and IFN gamma , so that keratinocytes could produce RANTES . ^^^ Stimulation with TNF alpha and IFN gamma in combination synergistically increased the RANTES production in this system . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These cells showed no significant expression of the beta chemokines RANTES ( regulated upon activation T cell expressed and secreted ) , MIP 1 alpha ( macrophage inflammatory protein 1 alpha ) , and MCP 1 ( monocyte chemotactic protein 1 ) under these conditions , but IFN gamma in combination with TNF alpha caused a dose dependent induction of RANTES and MCP 1 secretion . ^^^ In contrast , IFN gamma had no significant effect on TNF alpha stimulated IL 8 secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here we show that CD8+ T cells , isolated from the tumor infiltrating lymphocytes from human melanoma , synthesize type 1 cytokine ( IFN gamma and tumor necrosis factor alpha ) in a MHC class 1 restricted and tumor specific noncytolytic interaction with the autologous melanoma cells . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : There was a significant inhibition by dexamethasone ( from 1 to 100 nM ) on the secretion of monokines ( IL 1beta , IL 6 , IL 8 and TNF alpha ) and lymphokines ( IL 2 , IL 4 , IL 10 and IFN gamma ) , either after LPS or PHA stimulation ( P < 0 . 01 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human astroglioma cell lines and primary human fetal astrocytes were examined for VCAM 1 gene expression after treatment with proinflammatory cytokines ( TNF alpha , IL 1beta , IFN gamma ) in the absence or presence of TGF beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study compared the patterns of gene expression for several NK associated genes namely perforin ( PEF ) , granzymes A and B ( GA or B ) , IL 1beta , IFN gamma , tumor necrosis factor alpha ( TNF alpha ) , CD 16 and NK specific genes , NKG2A , NKG 5 and NKG 7 in both unstimulated and in IL 2 , IFN alpha and PMA / Ionomycin ( PMA / I ) stimulated NK cells purified from human peripheral blood . ^^^ IFN alpha enhanced mRNA expression for PEF , IFN gamma , TNF alpha and NKG2A , but did not affect NKG 7 mRNA expression . ^^^ IL 2 augmented mRNA expression for PEF , IFN gamma , TNF alpha , NKG2A and NKG 7 . ^^^ PMA / I increased mRNA expression for IFN gamma , TNF alpha and NKG2A but did not affect mRNA expression for PEF and NKG 7 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma induced TNF alpha is a prerequisite for in vitro production of nitric oxide generated in murine peritoneal macrophages by IFN gamma . ^^^ The effect of IFN gamma to stimulate formation of nitric oxide ( NO ) by normal murine peritoneal macrophages ( M phi ) has been found to be completely dependent on the ability of IFN gamma to activate secretion of TNF alpha . ^^^ The NO stimulatory effect of IFN gamma was abolished by anti TNF alpha antibodies , the inhibitory intervention of which could be fully reversed by exogenously supplied TNF alpha . ^^^ Accordingly , the failure of M phi from C3H / HeJ mice to secrete TNF alpha upon stimulation with IFN gamma was associated with their complete incapability to generate NO , unless they were simultaneously treated with IFN gamma + TNF alpha . ^^^ Collectively , the data document that similar to the NO up regulatory action of other cytokines , the effect of IFN gamma is not independent , but depends on a synergistic cooperation with the self produced TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma induced expression of HLA DR and DQ molecules and secretion of the monokines IL 1 beta and TNF alpha in THP 1 cells in a time and dose dependent manner . ^^^ Blocking of phospholipase D ( PLD ) derived diacylglycerol ( DAG ) formation by propranolol abrogated IFN gamma increased HLA class 2 expression and IL 1 beta secretion , but had little effect on IFN gamma induced TNF alpha production . ^^^ These findings appear to suggest that PLD derived phosphatidate is not the primary source of DAG production in IFN gamma induced TNF alpha secretion , but may be necessary for IFN gamma mediated MHC class 2 induction and IL 1 beta production in human monocytes , whereas phospholipase A 2 may not be required for IFN gamma activation of PKC in the process . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Evidence for the involvement of endothelial cell integrin alphaVbeta 3 in the disruption of the tumor vasculature induced by TNF and IFN gamma . ^^^ Administration of tumor necrosis factor ( TNF ) and gamma interferon ( IFN gamma ) to melanoma patients causes selective disruption of the tumor vasculature but the mechanism of this disruption is unknown . ^^^ Here we report that exposure of human endothelial cells to TNF and IFN gamma results in a reduced activation of integrin alphaVbeta 3 , an adhesion receptor that plays a key role in tumor angiogenesis , leading to a decreased alphaVbeta 3 dependent endothelial cell adhesion and survival . ^^^ Detachment and apoptosis of angiogenic endothelial cells was demonstrated in vivo in melanoma metastases of patients treated with TNF and IFN gamma . ^^^ These results implicate integrin alphaVbeta 3 in the anti vascular activity of TNF and IFN gamma and demonstrate a new mechanism by which cytokines control cell adhesion . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In each model , abnormal reconstitution of the T cell compartment in immunodeficient mice by adoptive cell transfer leads to a wasting syndrome and inflammation of the colon , induced by IFN gamma and tumor necrosis factor ( TNF ) alpha producing T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After a lethal challenge of H . capsulatum , CD40L / mice were not substantially different from CD40L+ / + mice in terms of mortality , fungal burden , or production of IFN gamma , IL 12 , nitric oxide , or tumor necrosis factor alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma was able to normalize TNF alpha production in Mphi from lupus prone mice , demonstrating a stimulus specific induction of the proinflammatory defects . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , CD 40 L neither affected HUVEC proliferation nor rescued them from IFN gamma and TNF alpha induced apoptosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both chemokines are produced in human umbilical vein endothelial cells ( HUVEC ) upon stimulation with IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In comparison to isografts from non CP injected mice , isografts from CP treated mice showed increased expression of IFN gamma , TNF alpha , TNF beta , IL 5 , and eotaxin but no increase in IL 10 expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The onset of collagen induced arthritis in DBA / 1 mice is accompanied by a predominantly Th 1 response , characterized by production of the proinflammatory cytokines IFN gamma and TNF alpha , and a predominance of IgG2a anti collagen Abs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The RT PCR technique was instrumental to determine the pattern of expression of pro inflammatory ( IL 1 beta , IL 6 , TNF alpha ) , Th 1 ( IFN gamma IL 2 ) , and Th 2 ( IL 4 ) cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Vgamma9Vdelta2 T cells stimulated with nonpeptidic mycobacterial antigens produce IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Peptide specific CD8+ T cell lines were cytotoxic , secreted IFN gamma and TNF alpha , but low to undetectable levels of IL 4 and IL 5 , and were able , upon adoptive transfer , to confer a high degree of protection against challenge infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro studies using bone marrow derived macrophages confirmed that rolipram directly suppressed TNF alpha and IL 12 production following stimulation with IFN gamma and LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increased IFN gamma production was demonstrated in anti CD 2 stimulated LPMC cultured in TNF alpha . ^^^ To determine the effects of cA 2 on cytokine production , TNF alpha and IFN gamma producing cells were quantitated in LPMC from five Crohn ' s disease patients treated with cA 2 . ^^^ In all four patients who demonstrated clinical and endoscopic improvement , decreased numbers of LPMC producing IFN gamma and TNF alpha following CD2 / CD28 activation paralleled improvement in disease activity over 8 wk . ^^^ In one patient who did not improve , increased numbers of TNF alpha and IFN gamma secreting LPMC were observed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Islet grafts and spleens from TNF alpha treated mice at 10 days after islet transplantation contained significantly fewer CD4+ and CD8+ T cells , and significantly decreased mRNA levels of type 1 cytokines ( IFN gamma , IL 2 , and TNF beta ) than islet grafts and spleens from control mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vivo depletion of NK cells by anti asialoGM 1 Ab or macrophage depletion by silicon dioxide treatment reduced abortion rates , which could no longer be boosted by injecting TNF alpha ( which activates NK cells ) or IFN gamma ( which activates macrophages ) . ^^^ Both spontaneous and cytokine boosted abortions in CBA 10 DBA / 2 were blocked by Ab to fgl 2 prothrombinase [ corrected ] expressed by cytokine stimulated vascular endothelial cells and monocytes ; in vivo Ab depletion of granulocytes also prevented TNF alpha + IFN gamma induced abortions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Perforin deficient CD8+ T cells provide immunity to Listeria monocytogenes by a mechanism that is independent of CD 95 and IFN gamma but requires TNF alpha . ^^^ Both lines specifically produce IFN gamma and TNF alpha , and mediate target cell lysis in vitro . ^^^ These results indicate that single Ag specific CD8+ T cells derived from PO mice can mediate antilisterial immunity by a mechanism that is independent of CD 95 or IFN gamma , but requires TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increased TNF alpha synthesis in vivo and in vitro and enhanced IFN gamma compared with IL 4 production in vitro in iNOS mutant mice demonstrated exaggerated Th 1 polarization of the host response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
During the early phase of infection , serum concentrations of IFN gamma , TNF alpha , IL 1beta , IL 4 , and IL 6 but not IL 2 increased dramatically . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Three manipulations resulted in alterations in the equilibrium between granulomas and liver microabscesses , with massive transfer of the infection to the hepatocyte and dissolution of the granulomas : depletion of neutrophils and neutralization of IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It has been demonstrated in vitro that immature crypt cell proliferation is stimulated by factors such as TGF alpha and TNF alpha , whereas TFG beta and IFN gamma inhibit mitotic activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN omega was assessed in comparison with IFN alpha 2c , IFN gamma , and TNF alpha with respect to production of HBV proteins and DNA in HepG2 . 2 . 15 cells , a HBV DNA transfected hepatoma cell line which produces infectious viral particles . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Plasma concentrations of IFN gamma and TNF alpha in psoriatic patients before and after local treatment with dithranol ointment . ^^^ IFN gamma and TNF alpha plasma levels were measured before and after local treatment in 27 patients . ^^^ Plasma concentrations of IFN gamma and TNF alpha were significantly higher before treatment ( 178 . 7 + / 11 . 9 pg / ml and 31 . 9 + / 11 . 6 pg / ml , respectively ) compared to the control group ( 139 . 6 + / 7 . 86 pg / ml and 17 . 1 + / 7 . 7 pg / ml , respectively ) . ^^^ After treatment IFN gamma levels were significantly decreased ( 151 . 3 + / 8 . 3 pg / ml ) toward the control group values and TNF alpha levels were observed even lower than in the controls ( 11 . 48 + / 6 . 8 pg / ml ) . ^^^ The data support the important proinflammatory role of IFN gamma and TNF alpha in the clinical manifestation of psoriasis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We previously demonstrated that iNOS can be induced in cultured bovine monocytes in response to IFN gamma and TNF alpha but lose this capability in a short period of time . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma acted in concert with LPS , TNF alpha , and IL 1beta in inhibiting CCR 2 expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The addition of TNFR IgG at the onset of cultures greatly reduced PWM induced tissue injury , without inhibiting the increase in TNF alpha and IFN gamma transcripts seen following T cell activation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , treatment of desensitized DC with TNF alpha plus prostaglandin E 2 fully restored CD 83 expression and partially restored IL 12 production as well as the IFN gamma inducing activity of DC in the mixed leukocyte reaction . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Concentrations of endotoxin , interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNFalpha ) , interleukin 1 ( IL 1 ) , and interleukin 6 ( IL 6 ) were measured . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) enhance lipopolysaccharide binding to neutrophils via CD 14 . ^^^ METHODS : Neutrophils were cultured with LPS after pretreatment with cytokines , interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 alpha ( IL 1alpha ) , or granulocyte colony stimulating factor ( G CSF ) . ^^^ RESULTS : LPS alone showed only slight binding to neutrophils , but pretreatment with IFN gamma or TNF alpha before LPS exposure markedly increased LPS binding and CD 14 expression on the surfaces of neutrophils . ^^^ CONCLUSIONS : These results demonstrate that IFN gamma and TNF alpha enhance LPS binding to neutrophils via CD 14 , suggesting that the priming effect of cytokines on neutrophils is important for LPS binding . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This report shows that P . carinii organisms , as well as L . monocytogenes , stimulated in whole spleen cell cultures of SCID mice the release of IFN gamma , TNF alpha / beta , IL 10 , IL 12 , and iNO . ^^^ Microbial induction of NK cell derived IFN gamma has been reported to be mediated by the combined effects of TNF alpha and IL 12 released by M phi upon adequate microbial stimulation . ^^^ In dose response experiments , release of IFN gamma , TNF alpha / beta , and iNO was reduced at high relative concentrations of either microorganism . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The BCs and the unfiltered and filtered BC PCs were assayed for the presence of cytokine mRNA ( i . e . , interleukin [ IL ] 1beta , IL 6 , tumor necrosis factor alpha [ TNFalpha ] , IL 8 , IL 2 , and interferon gamma [ IFN gamma ] ) by the use of competitive reverse transcription polymerase chain reaction . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Keratinocyte release of IL 1alpha , IL 1 receptor antagonist ( IL 1ra ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and tumor necrosis factor ( TNF ) alpha were measured by ELISA on culture supernatants after treatment with IFN gamma or medium alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To this end we analyzed the influence of LFA 3 density on melanoma cells on lysis and cytokine production ( TNF , IL 2 , IFN gamma ) by T cells following activation by various amounts of antigenic peptides . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
SEB evoked plasma levels of TNF alpha , IL 1beta , IL 2 , IL 6 , IL 12 and IFN gamma were significantly higher in the IL 10 KO mice than in the WT animals . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Differential potentiation of anti mycobacterial activity and reactive nitrogen intermediate producing ability of murine peritoneal macrophages activated by interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) . ^^^ The anti mycobacterial activities of IFN gamma and TNF alpha treated murine peritoneal macrophages were determined . ^^^ Resident macrophages pretreated with IFN gamma or TNF alpha for 2 days were infected with test organisms and subsequently cultured for up to 7 days . ^^^ Third , IFN gamma but not TNF alpha enhanced production of reactive nitrogen intermediates ( RNI ) by macrophages infected with M . tuberculosis or M . avium , whereas both cytokines increased macrophage release of reactive oxygen intermediates ( ROI ) . ^^^ The present findings therefore show that IFN gamma and TNF alpha potentiated the anti mycobacterial activity of murine peritoneal macrophages in different fashions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) release from RAW 264 and BMM in response to bacterial DNA was augmented by IFN gamma pretreatment . ^^^ Bacterial DNA has been shown to induce IFN gamma production in vivo as an indirect consequence of interleukin 12 ( IL 12 ) and TNF alpha production from macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have analysed TCR 5 alpha family usage , and the expression of interleukin 1alpha ( IL 1alpha ) , IL 1beta , IL 2 , IL 4 , IL 6 , IL 8 , IL 10 , IL 13 , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) , in labial biopsies from 12 patients with SS and compared these with samples from three patients with chronic sialadenitis ( CS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interestingly , VT superinduced TNF alpha and IL 6 production in LPS stimulated cells and also elevated TNF alpha in IFN gamma stimulated cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Whereas stimulation of CD 3 and CD 38 resulted in a dramatic induction of cytokine ( granulocyte macrophage CSF , IFN gamma , TNF alpha , and TNF beta ) release by both TALL 104 and LAK cells , ligation of CD38L was not followed by cytokine production in TALL 104 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These cells were examined for a coincident increase in cytokine production ( IFN gamma , TNF alpha , and granulocyte macrophage CSF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Protective effect on Leishmania major infection of migration inhibitory factor , TNF alpha , and IFN gamma administered orally via attenuated Salmonella typhimurium . ^^^ The genes encoding murine macrophage migration inhibitory factor ( MIF ) , IL 2 , IFN gamma or TNF alpha were cloned individually into an expression plasmid under the control of the inducible promoter nirB and transfected into the aroA aroD deletion mutant strain of Salmonella typhimurium ( BRD 509 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The lack of TNF receptors did not adversely affect the development of a type 1 IFN gamma response . ^^^ In vitro studies with peritoneal macrophages stimulated with IFN gamma and tachyzoites indicated that under limiting concentrations of IFN gamma , nitric oxide mediated toxoplasmastatic activity is TNF alpha dependent . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , two macrophage functions that are not induced by IFN gamma , basal electron transport and LPS induced TNF alpha production , were enhanced by treatment with CSC MS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Strong expression of IFN gamma , tumor necrosis factor alpha ( TNF alpha ) , TNF beta , and IL 2 receptor alpha chain mRNA was detected in T cell lines 48 h after antigen stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Optimal activation requires the presence of T cell derived gamma interferon ( IFN gamma ) and other signals , including tumor necrosis factor ( TNF ) . ^^^ When RAW264 . 7 cells were pretreated with human TNF or TNF ( 70 80 ) in the presence of IFN gamma , there was a dose dependent reduction in the replication of BCG as measured by the uptake of 3H labeled uracil and a concomitant release of nitric oxide as measured by the nitrite in the culture supernatants . ^^^ TNF or TNF ( 70 80 ) induced macrophage activation was dependent on IFN gamma and was inhibited by neutralizing monoclonal antibody to human TNF and by anti IFN gamma antisera . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this report we describe the cloning and sequence analysis of portions of the Syrian hamster interleukin 2 ( IL 2 ) , IL 4 , gamma interferon ( IFN gamma ) , tumor necrosis factor alpha , IL 10 , IL 12p40 , and transforming growth factor beta cDNAs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Therefore , we wanted to dissect the different mechanisms exerted by IL 12 during Yersinia infections by using different models of Yersinia resistant and susceptible mice , including resistant C57BL / 6 mice , susceptible BALB / c mice , intermediate susceptible wild type 129 / Sv mice , 129 / Sv IFN gamma receptor deficient ( IFN gamma R / ) mice and C57BL / 6 tumor necrosis factor ( TNF ) receptor p 55 chain deficient ( TNFR p 55 / ) mice . ^^^ While administration of IL 12 alone increased TNF alpha levels , administration of TGF beta or TGF beta plus IL 12 decreased both TNF alpha and IFN gamma levels in Yersinia infected C57BL / 6 mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Factors involved in regulating primary and secondary immunity to infection with Histoplasma capsulatum : TNF alpha plays a critical role in maintaining secondary immunity in the absence of IFN gamma . ^^^ It has been previously shown in a murine model of disseminated histoplasmosis that IL 12 , IFN gamma , and TNF alpha were important factors in mediating primary protection . ^^^ Moreover , normal C57BL / 6 mice treated with neutralizing Abs against either IL 12 , TNF alpha , or IFN gamma , depleted of neutrophils or treated with aminoguanidine at the time of reinfection , maintained an effective immune response . ^^^ It was further shown that enhancement of TNF alpha production in IFN gamma ( / ) mice was the major mechanism by which these mice were effective in controlling secondary infection . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interestingly , the most pronounced upregulation of inflammatory cytokines ( gamma interferon [ IFN gamma ] , tumor necrosis factor alpha [ TNF alpha ] and interleukin 12 ) is observed in spleen and lungs . ^^^ CASP surgery followed by stent removal at specific time intervals revealed that all animals survived if intervention was performed after 3 h , whereas removal of the septic focus after 9 h did not prevent death , suggesting induction of autonomous mechanisms of a lethal inflammatory response syndrome . 18G CASP surgery in IFN gamma receptor deficient ( IFNgammaR / ) mice revealed an essential role of IFN gamma in survival of sepsis , whereas TNF receptor p 55 deficient ( TNFRp 55 / ) mice did not show altered survival rates . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Experiments were carried out to determine if , when , and where tumor necrosis factor ( TNF ) and gamma interferon ( IFN gamma ) function in antibacterial resistance during enteric listeriosis . ^^^ By the third day of infection , bacterial numbers in infected tissues and organs were greatly exacerbated in all mice treated with anti TNF MAb , whereas bacterial numbers in the organs of mice treated with anti IFN gamma MAb did not differ from those present in the respective organs of control mice . ^^^ Experiments with Listeria immune mice revealed that TNF and IFN gamma are involved in the expression of anti Listeria memory immunity ; however , it was also found that the anti IFN gamma MAb was relatively ineffective in inhibiting the expression of anti Listeria immunity , whereas a polyclonal anti IFN gamma was quite effective . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Addition of IFN alphabeta to DC / T cell cultures resulted in induction of T cell IL 10 production and inhibition of IFN gamma , TNF alpha , and LT secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The IL 10T mice displayed a significant increase in hepatic granuloma size at the acute stage of infection , which was associated with increased IFN gamma , IL 2 , IL 1beta , and TNF alpha mRNA expression in liver and elevated Th 1 type cytokine production by lymphoid cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We show that 1 ) the appearance of the Th 1 associated cytokines IFN gamma and IL 12 in bronchoalveolar lavage fluid is caused by unmethylated CpG dinucleotide sequences present within the plasmid , and is enhanced by the lipid formulation ; 2 ) cationic lipids by themselves do not induce IL 12 or IL 12p40 ; 3 ) TNF alpha is rapidly induced by cationic lipids and plasmid / lipid complex , but not by plasmid alone ; 4 ) an acute cellular influx is induced by cationic lipid alone and by a plasmid / lipid complex , but to a much lesser extent by plasmid alone ; and 5 ) plasmid methylation does not influence the degree of inflammatory cell influx . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found that human umbilical cord endothelial cells ( HUVEC ) stimulated with TNF alpha and IFN gamma secreted eosinophil chemoattractants into the culture supernatant . ^^^ Eosinophils accumulated efficiently on HUVEC stimulated with TNF alpha and IFN gamma in shear flow at 1 . 5 dyn / cm2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After incubation of macrophages with dantrolene ( 1 to 100 microM ) and LPS ( 1 microgram . ml 1 ) and IFN gamma ( 100 u . ml 1 ) for 24 hr , the cell free medium was removed for measuring the nitrite and tumour necrosis factor alpha ( TNF alpha ) levels by Griess reaction and ELISA kit , respectively . ^^^ RESULTS : In rats alveolar macrophages , ( 1 ) dantrolene ( 1 to 100 microM ) caused a dose dependent suppression of the production of nitrite and TNF alpha induced by LPS ( 1 microgram . ml 1 ) plus IFN gamma ( 100 u . ml 1 ) and ( 2 ) dantrolene ( 100 microM ) inhibited the activity ( by 37 + / 5 % , P < 0 . 01 ) and protein expression ( by 39 + / 12 % , P < 0 . 01 ) of iNOS in response to LPS plus IFN gamma . ^^^ CONCLUSION : Dantrolene inhibits NO production as well as the activity and expression of iNOS in alveolar macrophages treated with LPS plus IFN gamma , which may be associated with the reduction of TNF alpha production . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Comparable TNF alpha , IFN gamma and GM CSF production by purified normal marrow CD 3 cells in response to horse anti lymphocyte and rabbit antithymocyte globulin . ^^^ In this study we compare the in vitro priming effect of HALG and RATG on purified normal marrow T cells : end points of the study were 1 ) levels of TNF alpha ( TNF alpha ) , IFN gamma ( IFN gamma ) GM CSF in T cell supernatants , and 2 ) effect of T cell supernatants on colony formation with or without exogenous GM CSF TNF alpha , IFN gamma and GM CSF levels were comparable for HALG , RATG and phytohaemagglutinin ( PHA ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha , also produced by several T cell clones , may in conjunction with IFN gamma have a toxic effect or enhance the IFN gamma induced increase of HLA class 2 expression on surface enterocytes . ^^^ Th 1 cells also activate cytotoxic CD8+ T cells that migrate in the epithelial layer , and stimulate further LPL macrophages to produce IFN gamma and TNF alpha enhancing the inflammatory response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma activates other resting macrophages , which , in turn , release cytokines , such as IL 1beta , TNF alpha , and free radicals , which are toxic to beta cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
At that time , cells in BM of B 6 and CBA / J injected recipients were augmented in intracellular IL 4 , IL 10 , and TNF alpha , whereas only cells in B 6 treated BM were increased in IFN gamma , and both treated groups of mice had up regulated endogenous MHC class 1 and class 2 expression in the three organs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The most relevant to the peripheral nerves are tumor necrosis factor ( TNF alpha ) , interleukin 1 ( IL 1 ) , interferon gamma ( IFN gamma ) and IL 12 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
AIM : The purpose of the investigation was to evaluate the presence of IL 1 beta , IL 2 , IL 6 , IL 8 , TNF alpha , IFN gamma in aqueous humor of patients with cataract . ^^^ RESULTS : IL 2 , IL 6 , IL 8 , TNF alpha and IFN gamma were found in all aqueous humor samples . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Using sensitive radioimmunoassays , interleukin 4 ( IL 4 ) , IL 10 , IL 12 p 40 , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and granulocyte macrophage colony stimulating factor ( GM CSF ) released into the culture supernatants of various unstimulated and stimulated blood mononuclear cell populations from 10 SLE patients was assessed in comparison with 10 matched healthy controls studied in parallel . ^^^ Lymphocyte enriched cells in SLE produced decreased amounts of IFN gamma and TNF alpha following stimulation . ^^^ Depletion studies suggested that CD8+ cells were a major source of TNF alpha and IFN gamma in controls , but not in SLE patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We further found that polymorphonuclear neutrophils isolated from normal donors produce IFN gamma in response to stimulation with LPS , IL 12 , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The main effector cells involved are CD4+ T cells , recognizing encephalitogenic epitopes within the central nervous system , and macrophages , both of which secrete proinflammatory cytokines , such as IFN gamma and TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These short peptides reduced production of IFN gamma and TNF alpha in vivo . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To study adoptive immunotherapy for adenoid cystic carcinoma of the salivary gland , we evaluate the influence of TNF alpha or IFN gamma on the activity of LAK cells when they acted at different stages of LAK cells killing target cells . ^^^ Both TNF alpha and IFN gamma could be an enhancer to augment IL 2 induced LAK cytotoxity against ACC 2 , TNF alpha should be used to induce LAK cells , but IFN gamma is suitable to pretreat target cells ACC 2 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The acute phase response is triggered by changes in intracellular mediators that activate stress sensitive kinases and transcription factors controlling the synthesis of proinflammatory cytokines such as TNF alpha , IL 1 , IL 8 or IFN gamma . 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment with tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 beta ( IL 1 beta ) or a combination of interferon gamma ( IFN gamma ) , TNF alpha , and IL 1 beta caused significant increases in the IR ET levels in the culture medium . ^^^ The expression levels of ET 1 mRNA were increased by treatment with a combination of IFN gamma , TNF alpha , and IL 1 beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , stimulation of AM from CAP patients with LPS plus IFN gamma augmented TNF alpha and IL 6 cytokine release to near normal levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Methylcellulose cultures of BM cells from Fac / and Fac+ / + mice were established to examine the growth of multipotent and lineage restricted progenitors containing inhibitory cytokines , including interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and macrophage inflammatory protein 1alpha ( MIP 1alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The regulation of prion protein ( PrP ) gene expression by a panel of glial and neuronal cytokines ( TNF alpha , IFN gamma , IL 1beta , IL 10 , and TGF beta 1 ) was investigated in human neural cell lines by reverse transcription polymerase chain reaction and Northern blot analysis . ^^^ In SK N SH cells , a 48 hour ( h ) treatment with 100 ng / ml IL 1beta , 100 ng / ml TNF alpha , or 100 nM phorbol 12 myristate 13 acetate induced a 2 . 7 to 4 . 2 fold increase in the level of PrP mRNA , while the exposure to 100 ng / ml IFN gamma resulted in a 50 % decrease . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Fab SEA therapy induced substantial levels of IFN gamma and TNF alpha in serum . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The aim of this study was to analyze the influence of topical FK 506 on molecular ( IL 1alpha , IL 1beta , IL 2 , IL 4 , IL 12 p 35 , IL 12 p 40 , macrophage inflammatory protein 2 ( MIP 2 ) , granulocyte macrophage CSF ( GM CSF ) , TNF alpha , and IFN gamma ) and cellular ( 1 A+ / CD80+ , 1 A+ / CD54+ , 1 A+ / CD69+ , 1 A+ / B220+ , and CD4+ / CD25+ ) events in epidermal ( EC ) and local draining lymph node ( LNC ) cells during primary contact hypersensitivity responses . ^^^ Cytokine mRNA levels for IL 1alpha , IL 1beta , GM CSF , TNF alpha , MIP 2 , and IFN gamma in EC and for IL 2 , IL 4 , IL 12 p 35 , IL 12 p 40 , and IFN gamma in LNC were increased and resulted in significant LNC proliferation during oxazolone induced contact hypersensitivity . ^^^ This effect was correlated with decreased IL 1alpha , IL 1beta , GM CSF , TNF alpha , MIP 2 , and IFN gamma mRNA expression within the epidermis and decreased IL 12 p 35 and p 40 mRNA expression in LNC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cross linking of NK1 . 1 with immobilized mAb induced granule release and TNF alpha , IFN gamma , and GM CSF secretion ; all were inhibited by coimmobilized anti Ly 49A . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine mRNA expression varied individually over a wide range ; TNF alpha , IL 6 , and IFN gamma were detectable in > 90 % of lesions , IL 12 and IL 10 in 40 % and 70 % , respectively , and IL 4 in only 9 % . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Thus , in the absence of IFN gamma and / or TNF / LT alpha , exhaustion of virus specific T cells was not hampered . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The addition of IFN gamma and TNF alpha to human keratinocytes in culture up regulated the expression of ICAM 1 and HLA DR on the cell surface . ^^^ When keratinocytes were treated with PUVA prior to the addition of IFN gamma and TNF alpha , ICAM 1 and HLA DR expression was suppressed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In order to investigate whether heparin influences the humoral immunity by interacting with cellular elements and affecting gene expression in blood circulating cells . we studied the effect of heparin on IL 1beta , IL 6 and TNFalpha mRNAs in human lipopolysaccharide ( LPS ) or interferon gamma ( IFN gamma ) stimulated mononuclear cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation with cytokines ( IL 1 beta , TNF alpha , and IFN gamma ) increased the expression of iNOS mRNA detected by reverse transcriptase polymerase chain reaction ( RT PCR ) in cultures of normal ( 16HBE14o ) , but not CF ( CFBE41o , with delta F 508 CFTR mutation ) epithelial cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Large quantities of IL 8 , TNF alpha , granulocyte macrophage colony stimulating factor ( GM CSF ) and IL 3 were secreted time dependently over a 72 hr period , with lower levels of IL 1 beta , 6 , 10 and TGF beta and no detectable IL 2 , 4 and IFN gamma protein . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To investigate the latter , the authors performed measurements of lipopolysaccharide ( LPS ) stimulated whole blood , interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) , interleukin 1 ( IL 1 ) and IL 12 production in 13 healthy volunteers over 24 h . ^^^ Elevation of plasma cortisol within the physiological range by administration of cortisone acetate , 25 mg at 21 . 00 , markedly suppressed IFN gamma , TNF alpha , IL 1 and IL 12 production , but not the later early morning rise of endogenous plasma cortisol . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Psychological stress significantly increased the stimulated production of tumour necrosis factor alpha ( TNF alpha ) , interleukin 6 ( IL 6 ) , IL 1 receptor antagonist ( IL 1Ra ) , interferon gamma ( IFN gamma ) and IL 10 . ^^^ Students with high stress perception during the stressful condition had a significantly higher production of TNF alpha , IL 6 , IL 1Ra and IFN gamma than students with a low stress perception . ^^^ These findings suggest that , in humans , changes in the production of the pro inflammatory cytokines , TNF alpha , IL 6 and IFN gamma , and negative immunoregulatory cytokines , IL 10 and IL 4 , take part in the homeostatic responses to psychological stress and that stress induced anxiety is related to a T helper 1 like response . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In order to test this hypothesis , cells from the murine lung epithelial cell line LA 4 were cultured to confluence and stimulated to express iNOS and produce NO by cytomix , a combination of human tumor necrosis factor alpha ( TNF alpha ) , human interleukin 1beta ( IL 1beta ) and murine interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cathepsin D ( CD ) , the major intracellular aspartyl protease , is a mediator of IFN gamma and TNF alpha induced apoptosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lymphocytes isolated from peripheral blood were activated by Conconavalin A ( Con A ) with and without the addition of p 43 and the concentrations of cytokines ( IL 2 , TNF alpha , IFN gamma , IL 4 , IL 10 and IL 6 ) secreted into the culture medium were determined . ^^^ No significant differences were found between the two groups regarding the levels of IL 2 , TNF alpha , IFN gamma and IL 4 . ^^^ Cytokine concentrations were analyzed according to the type 1 / type 2 cytokine profile ( IL 2 , TNF and IFN gamma and IL 4 , IL 6 and IL 10 , respectively ) . ^^^ This analysis revealed no significant differences in IL 2 , TNF or IFN gamma between benign and malignant tumors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro cytokine production by patient PBMCs showed 75 % less PHA induced IFN gamma production than in normal cells , while patient PHA induced TNF alpha production was normal . ^^^ The normal augmentation of TNF alpha production when IFN gamma was added to endotoxin was absent from patient cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We tested effects of alpha MSH ( 1 13 ) , alpha MSH ( 11 13 ) , and ACTH ( 1 24 ) on production of tumor necrosis factor alpha ( TNF alpha ) , interleukin 6 ( IL 6 ) , and nitric oxide ( NO ) in a cultured murine microglial cell line ( N 9 ) stimulated with lipopolysaccharide ( LPS ) plus interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
VM induced the secretion of Th 1 ( IFN gamma ) or Th 2 ( IL 4 ) related cytokines and also the release of TNF alpha and IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Clinical Observation The concentration of MPO , TNF alpha , and IFN gamma in peripheral blood of 37 cases of chronic airway infection were observed . ^^^ Experimental Observation One hundred twenty two of alginate immunized mice were made by 5 times of intraabdominal injection with purified alginate extracted from mucoid P . aeruginosa PT 1252 . 4 10 10 ( 6 ) CFU / body of mucoid P . aeruginosa PT 1252 or 20 micrograms / body of purified alginate was intubated per trachea into the immunized mice , and changes in mean fluorescence intensity ( MFI ) of MPO and TNF alpha on neutrophils and IFN gamma on CD 3 positive lymphocytes in BALF were observed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IgG antibody subclasses , tumor necrosis factor and IFN gamma levels in patients with type 2 lepra reaction on thalidomide treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The myelosuppressive effect of supernatants of gamma delta T cells could be inhibited by antibodies against IFN gamma or TNF alpha . ^^^ Accordingly , we found increased numbers of TNF alpha or IFN gamma secreting CD8+ gamma delta T cells in HIV+ patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma acted synergistically with TNF alpha to increase RANTES production . ^^^ RANTES is produced in vitro by RPE in response to the proinflammatory cytokines IL 1 beta , TNF alpha , and IFN gamma and is therefore likely to play a role in the development of the inflammatory eye disease endogenous posterior uveitis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma therapy has been associated with high levels of tumor necrosis factor alpha ( TNF alpha ) , with potential enhancement of coagulopathy after injury . ^^^ This study evaluated TNF alpha production and markers of hemostatic activation in patients receiving IFN gamma therapy . ^^^ We conclude that ( 1 ) IFN gamma therapy at the dose employed was not associated with a significant increase in TNF alpha or other inflammatory cytokine production beyond that seen in patients receiving placebo , ( 2 ) coagulation and fibrinolytic markers were increased following injury but decreased significantly in surviving patients , and ( 3 ) no changes in coagulation and fibrinolytic parameters were noted in relation to IFN gamma therapy . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Patients in both treatment arms were included , because for these patients anti CD 4 treatment in this study did not affect TNF alpha and IFN gamma production and did not reduce signs of disease activity on magnetic resonance imaging . ^^^ MAIN OUTCOME MEASURE : Distribution of classes of TNF alpha and IFN gamma production ( expressed as z scores ) in patients with or without clinical or magnetic resonance imaging signs of disease activity . ^^^ RESULTS : One month preceding exacerbations of multiple sclerosis , there was a shift toward higher z scores of TNF alpha production ( P < . 05 ) , but not of IFN gamma production . ^^^ There was no statistically significant relationship between IFN gamma and TNF alpha production and magnetic resonance imaging markers of multiple sclerosis activity . ^^^ CONCLUSION : The production of TNF alpha , and not of IFN gamma , is significantly higher in patients with multiple sclerosis before exacerbations than in patients with stable disease . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In order to investigate the the effects of transferrin ( Tf ) on the production of cytokines , mouse spleen cells were treated with various concentrations of apo and holo Tf , and then the production of IL 6 , IFN gamma and the expression of mRNA for TNF alpha was determined . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These clinical effects were associated with dose dependent down modulation of myelin antigens induced T cell responses and by suppression of the proinflammatory cytokines IFN gamma and TNF alpha , and upregulation IL 4 , IL 10 and TGF beta as evaluated by in situ hybridization for mRNA expression in spleen mononuclear cells and spinal cord sections . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
DESIGN AND METHODS : Linomide inhibits production of proinflammatory cytokines such as TNF alpha , interleukin 1 beta and IFN gamma , as well as iNOS synthesis . ^^^ Linomide also prevents human TNF alpha and IFN gamma production , as well as iNOS expression and affects the viral load , promoting potent suppression of HIV 1 infectivity as detected in peritoneal cavity and spleen . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma production of adult rat astrocytes triggered by TNF alpha . ^^^ IFN gamma was hardly detectable in cultured astrocytes , while addition of TNF alpha dose dependently induced IFN gamma production by astrocytes . ^^^ TNF alpha induced IFN gamma production by astrocytes was inhibited by treatment of astrocytes with TGF beta 1 , but not IL 10 . ^^^ TNF alpha induced IFN gamma production by astrocytes was confirmed by using immunocytochemical staining . ^^^ The data suggest that astrocyte derived IFN gamma induced by TNF alpha may participate in local immune reactions of the brain in an autocrine and paracrine fashion . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1beta and TNF alpha , but not IFN alpha , IFN gamma , IL 6 or IL 8 , are secretory mediators in human distal colon . ^^^ By their secretory effects IL 1beta and TNF alpha , but not IFN alpha , IFN gamma , IL 6 , and IL 8 , may contribute to diarrhoea in IBD and AIDS . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , IFN gamma appeared to completely override the ICAM 1 upregulation induced by IL 1 beta , IL 8 and TNF alpha , during HRV infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines IL 4 , TNF alpha , and IFN gamma were produced by 91 . 7 % , 29 . 2 % , and 27 . 4 % of the cells , respectively . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We first determined that surface LAG 3 expression on activated human T cells is upregulated by certain cytokines ( IL 2 , IL 7 , IL 12 ) and not by others ( IL 4 , IL 6 , IL 10 , TNF alpha , TNF beta , IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found elevated levels of interleukin 6 ( IL 6 ) , interleukin 10 ( IL 10 ) , tumor necrosis factor ( TNF ) , and interferon gamma ( IFN gamma ) in Mev mice sera and cultured B and T cells compared to littermate control adult mice . ^^^ To determine if NF kappa B had a role in causing the elevated levels of cytokines in Mev mice , we treated activated Mev T cells with an NF kappa B decoy and found that cell culture treatment with the decoy resulted in significant reduction of the secretion of IL 6 , GM CSF , and TNF , but not IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pro alpha 1 stimulated alone or in combination with IFN gamma the TNF alpha and IL 1beta secretion by monocytes and decreased the high PGE 2 and TGF beta level , especially in the patients groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both TNF alpha and IL 10 have been found to be major factors determining enhancing effects of PMEG , ( R ) PMPA , and ( S ) PMPA on production of NO generated by exogenous IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro differentiation , TNF alpha and IFN gamma production , and expression of the cytolytic effector molecules perforin and Fas L were comparable among IL 2 activated NK cells from LFA 1 ( / ) , CD 44 ( / ) , CD 44 ( / ) LFA 1 ( / ) , and control mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We now report on the effect of gammadelta T cell depletion on expression of five cytokine genes , IL 1 , IL 6 , TNF , lymphotoxin , and IFN gamma in spinal cords of mice during the pre onset , onset , height , and recovery phases of EAE , and on expression of type 2 nitric oxide synthase . ^^^ In control animals , the mRNAs for IL 1 and IL 6 rose dramatically at disease onset and peaked before disease height , whereas the mRNAs for TNF , lymphotoxin , and IFN gamma rose more slowly and peaked with peak of disease . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Influenza A virus infected human peripheral macrophages secreted IFN alpha / beta , TNF alpha , IL 1beta , and a recently identified cytokine , IL 18 ( or IFN gamma inducing factor ) , whereas the production of IL 12 was not detected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Levels of IFN gamma and granulocyte macrophage CSF were elevated in TNF alpha neutralized naive mice , whereas IL 4 , 6 , 10 and TGF beta did not differ from controls . ^^^ Alveolar macrophages ( Mphi ) did not exert fungistatic activity against Hc after exposure to recombinant murine TNF alpha , recombinant murine IFN gamma , or both . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The type 1 cytokines IL 12 , IFN gamma , and TNF alpha , but not the type 2 cytokines IL 4 and granulocyte macrophage ( GM ) CSF , markedly increased in the lung and peripheral blood of wt mice postinfection , which resulted in the development of intense granulomatous responses and the effective control of mycobacterial infection in the lung . ^^^ This cytokine , together with IFN gamma and TNF alpha , and granulomatous inflammation are critically required for the effective control of pulmonary mycobacterial infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1beta and TNF alpha in combination with IFN gamma markedly increased endothelial C 3 deposition ; however , immunofluorescence microscopy revealed that the endothelial cells had retracted , and that bound C 3 was concentrated not on cells but in areas of exposed subendothelial extracellular matrix ( ECM ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Following the interaction , LAK cells are activated : an increased expression of some cytokine mRNA ( in particular , TNF alpha , GM CSF , and IFN gamma ) has been revealed by RT PCR and perforin secretion has been suggested by immunofluorescence microscopy . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The transformed cell line was compared with the parental proximal tubular epithelial cells in its ability to respond to inflammatory cytokines such as IL 1alpha TNF alpha , IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Examination of cytokine expression in the whole elicited cell population revealed that both P . gingivalis elicited cells and TG elicited cells expressed messenger RNA for interleukin 2 ( IL 2 ) , TNF alpha and interferon gamma ( IFN gamma ) , but not for IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Anti CD 3 restimulated T cells ( mainly CD8+ ) produced IL 2 , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) and low levels of IL 4 and IL 10 transcripts and proteins . ^^^ Anti CD 3 stimulated IL 2 gene expression was down regulated by protein synthesis inhibitor , whereas IL 10 , IFN gamma and TNF alpha genes were readily induced independent of ongoing protein synthesis . ^^^ In conclusion , the cytokines synthesized by IL 2 dependent T cells were predominantly IL 2 , IFN gamma and TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , interleukin ( IL ) 1 beta , IL 2 , IL 4 , IL 5 , IL 6 , IL 8 , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) showed increased BF levels as compared with BP sera . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We also analysed the production of IL 10 which is endowed with inhibitory effects on IL 2 and IFN gamma synthesis , and of TNF alpha , a pleiotropic inflammatory cytokine . ^^^ On ionomycin + PMA stimulation , which reveals the intrinsic potential of lymphokine production by T cells , the CD57+ T cell subsets from all individuals produced high amounts of IFN gamma and TNF alpha mRNA and protein . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have studied the cytotoxic mechanism induced by this anti CD 3 IT as well as its effects on proliferation , phenotypic changes and cytokine production ( IL 2 , IFN gamma and TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Activated Th 1 cells can produce interleukin 2 ( IL 2 ) , interferon gamma ( IFN gamma ) , and tumor necrosis factor ( TNF ) , whereas Th 2 cells produce IL 4 and IL 13 , which inhibit Th 1 cells . ^^^ Likewise , combination of these cytokines with IL 1 alpha , IFN gamma , or TNF alpha , respectively , did not result in detectable RANTES production . ^^^ IL 4 or IL 13 in combination with IL 1 alpha + IFN gamma or IFN gamma + TNF alpha resulted in a two to fourfold augmentation of RANTES production , ranging from 2 . 2 + / 0 . 2 to 35 + / 2 ng / ml in different cell lines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cells from the inflamed colonic LP of transplanted scid mice produced high amounts of IL 12 , IFN gamma and TNF alpha but only low amounts IL 4 and IL 10 . ^^^ Intracellular cytokine staining confirmed the presence of large numbers of IFN gamma and TNF alpha producing effector CD4+ T cells in the colonic LP of scid mice with colitis but also in non inflamed tissues [ spleen ( S ) , peritoneal cavity ( PC ) and mesenteric lymph nodes ( mLN ) ] of the adoptive host . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We demonstrate that protection was associated with an acquired unresponsiveness of lymphocytes to MBP and a decreased ability of the cells to produce IL 2 , IFN gamma and TNF alpha and , to a lesser degree , IL 4 and IL 10 , in the presence of the antigen . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Peak production of the pro inflammatory cytokines IFN gamma , TNF alpha , IL 1 and IL 12 occurs during the night and early morning at a time when plasma cortisol is lowest . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Susceptibility to Pneumocystis carinii in mice is dependent on simultaneous deletion of IFN gamma and type 1 and 2 TNF receptor genes . ^^^ The cytokines IFN gamma and TNF have contributory roles in host defense against P . carinii , but their combined and interactive importance is unclear . ^^^ To test the roles of these cytokines in defense against P . carinii directly , organisms were inoculated intratracheally into wild type mice and into three groups of gene deleted mice : those lacking genes for IFN gamma ( IFN gamma ( / ) ) , for TNF receptors 1 and 2 ( TNFR ( / ) ) , and for both IFN gamma and TNFR ( TNFR IFN gamma ( / ) ) . ^^^ Our findings demonstrate conclusively that deletion of either IFN gamma or TNF activity alone does not block clearance of P . carinii . ^^^ However , simultaneous deletion of IFN gamma and TNF receptor genes results in susceptibility to P . carinii . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Blood derived , highly purified human EOS from six atopic patients and two nonatopic individuals were treated in culture with IL 4 , IL 5 , granulocyte macrophage CSF , IFN gamma , TNF alpha , IL 1alpha , RANTES , and complement 5a , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Under these conditions , CD8+ cells produced proinflammatory cytokines including IFN gamma , TNF alpha , and IL 2 , which were significantly elevated in HAM / TSP patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In MRL Fas ( lpr ) mice lacking IFN gamma R , circulating and intrarenal CSF 1 were absent , TNF alpha was markedly reduced , survival was extended , lymphadenopathy and splenomegaly were prevented , and the kidneys remained protected from destruction . ^^^ Mesangial cells ( MC ) that were signaled through the IFN gamma R induced CSF 1 and TNF alpha in MRL Fas ( lpr ) mice . ^^^ In conclusion , IFN gamma R signaling is essential for the initiation ( CSF 1 ) , acceleration ( CSF 1 and TNF alpha ) , and apoptotic destruction of renal parenchymal cells in MRL Fas ( lpr ) autoimmune kidney disease . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mesothelial cells were cultured and stimulated with either IL 1beta , tumour necrosis factor alpha ( TNF alpha ) or IFN gamma or combinations of these . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These data argue for a major role of the proinflammatory cytokines TNF alpha , IL 1beta , IL 6 , IL 12 , IFN gamma and cytolysin in initiation and perpetuation of autoimmune sialoadenitis in MRL / lpr mice , probably in conjunction with an insufficiency of the anti inflammatory cytokines TGF beta and IL 10 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Analysis with reverse transcriptase polymerase chain reaction ( RT PCR ) of cytokine gene expression revealed both Th 1 and Th 2 like products in all TCL : IL 2 product ( in 17 TCL ) , interferon gamma ( IFN gamma ) ( n = 10 ) , tumour necrosis factor beta ( TNF beta ) ( n = 15 ) , IL 4 ( n = 12 ) , IL 5 ( n = 17 ) , IL 6 ( n = 13 ) , TNF alpha ( n = 12 ) and IL 10 ( n = 4 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Detection of Fcgamma receptors on human endothelial cells stimulated with cytokines tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) . ^^^ TNF alpha and IFN gamma significantly increased the expression of FcgammaR type 2 ( FcgammaRII ) and type 3 ( FcgammaRIII ) on aortic EC . ^^^ Simultaneous treatment with both cytokines had a synergistic effect and pretreatment of EC with IFN gamma augmented the effect of TNF alpha . ^^^ This study showed that the inflammatory cytokines TNF alpha and IFN gamma enhanced low affinity FcgammaR expression on human EC in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Production of host protective ( IFN gamma ) , host impairing ( IL 10 , IL 13 ) and inflammatory ( TNF alpha ) cytokines by PBMC from leprosy patients stimulated with mycobacterial antigens . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunohistochemistry was performed using monoclonal antibodies ( MAb ) specific for CD 3 , CD 4 , CD 8 , CD 22 , CD 25 , CD 38 , CD 68 , MAb 67 , Ki 67 , interferon gamma ( IFN gamma ) , interleukin ( IL ) 1alpha , IL 1beta , tumour necrosis factor alpha ( TNF alpha ) , E selectin , ICAM 1 and VCAM 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of SVHK cells with anti Fas antibody or TNF alpha in the presence of interferon gamma ( IFN gamma ) resulted in an increase in Mn SOD activity , Cu , Zn SOD activity was not affected . ^^^ The induction of IFN gamma dependent Mn SOD activity by anti Fas antibody or TNF alpha was concentration dependent ; the maximal effect was observed at 1 10 micrograms / ml and 5 10 ng / ml , respectively . ^^^ The addition of both anti Fas antibody and TNF alpha in the presence of IFN gamma resulted in an additive increase in Mn SOD activity . ^^^ Although the addition of 12 o tetradecanoylphorbol 13 acetate ( TPA ) singly to the incubation medium had no effect on either Mn , or Cu , Zn SOD activity , it significantly augmented the IFN gamma dependent induction of Mn SOD activity by anti Fas antibody or by TNF alpha . ^^^ These results indicate that the stimulation of Fas antigen or TNF receptor increases Mn SOD activity of SVHK cells in the presence of IFN gamma and that TPA augments the process through the activation of protein kinase C . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The intraperitoneal administration of endotoxin in mice resulted in the accumulation of leucocytes in the peritoneal cavity and in the increase of IL 1 , IL 6 , TNF alpha and IFN gamma concentration in serum . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These results suggest that the capacity of TOAE to restore NO production from interferon gamma ( IFN gamma ) primed mouse peritoneal macrophages is the result of TOAE induced TNF alpha secretion . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Proinflammatory cytokines , a combination of IL 1beta , TNF alpha , and IFN gamma , caused mRNA expression of GTP cyclohydrolase 1 ( GTP CH ) , the rate limiting enzyme in tetrahydrobiopterin ( BH 4 ) biosynthesis , and of inducible nitric oxide synthase ( iNOS ) in a well characterized osteoblastic clone MC3T3 E 1 cell line . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both pro inflammatory and anti inflammatory cytokines were produced within individual lesions during active disease ( IFN alpha , IFN gamma , TNF alpha , IL 1alpha , IL 1beta , IL 2 , IL 4 , IL 10 and IL 12 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The present innate resistance exhibited in SCID mice is discussed in relation to cell mediated immunity of macrophage activation by IFN gamma which would not involve T lymphocytes but is initiated by IL 12 and TNF alpha cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the spleen and lymph node ( LN ) of resistant B 6 mice , IL 2 , IFN gamma and TNF alpha were induced rapidly with large numbers of cells producing these cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Densitometric analyses indicated that , in infected animals , MLN expressed significantly ( p < 0 . 05 ) more mRNA for TNF alpha : JPP had significantly increased ( p < 0 . 05 ) mRNA for GM CSF and significantly decreased ( p < 0 . 05 ) mRNA for IL 4 and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Thus , VEGF protein in culture supernatant was measured by using enzyme linked immunosorbent assay ( ELISA ) with or without inflammatory cytokines , i . e . , interleukin ( IL ) 1beta , interferon ( IFN ) alpha , IFN gamma , and tumor necrosis factor ( TNF ) alpha ; and growth factors , i . e . , epidermal growth factor ( EGF ) , platelet derived growth factor ( PDGF ) BB , basic fibroblast growth factor ( bFGF ) , and transforming growth factor ( TGF ) alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , IL 2 , tumor necrosis factor alfa ( TNF alpha ) , and interferon gamma ( IFN gamma ) had no suppressive effect on HBV envelope protein expression in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found no expression of IL 1RA , IL 2 , IL 4 , IL 13 , TNF alpha , or IFN gamma in HCAEC . ^^^ IL 1beta and TNF alpha synergistically induced IL 6 and GM CSF and additively induced IL 8 and MCP 1 production , while IL 2 , IL 10 , IFN alpha , and IFN gamma had little or no additional effects . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TCR 5 beta peptide injection into young mice had no effect on T or B cell mitogenesis and IL 4 production while modifying tumour necrosis factor alpha ( TNF alpha ) , IL 6 , and interferon gamma ( IFN gamma ) secreted by mitogen stimulated spleen cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Macrophages activated by low density lipoproteins ( LDL ) secrete tumor necrosis factor alpha ( TNF alpha ) and interleukin 1 ( IL 1 ) in vitro , while LDL stimulated T lymphocytes release interferon gamma ( IFN gamma ) . ^^^ The aim of this study was to estimate the plasma levels of TNF alpha , IL 1 , IFN gamma in patients with hyperlipoproteinemia IIb ( HLPIIb ) and atherosclerosis . ^^^ The decreased levels of TC , TG , and LDL correlated with the decreased levels of TNF alpha and IFN gamma . ^^^ CONCLUSION : The results of this study indicate that plasma TNF alpha and IFN gamma levels in hyperlipidemic patients are higher than in healthy subjects , and that fenofibrate is effective in decreasing lipids and cytokines in plasma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We previously reported upregulation of gene expression for a number of proinflammatory cytokines , interleukin 1beta ( IL 1beta ) , IL 2 , IL 6 , tumor necrosis factor alpha ( TNF alpha ) , TNF beta , and interferon gamma ( IFN gamma ) , in the CNS of mice with myelin basic protein ( MBP ) induced relapsing EAE by using semiquantitative reverse transcriptase polymerase chain reaction ( RT PCR ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Evaluation of various cytokines ( IL 6 , IFN alpha , IFN gamma , TNF alpha ) as markers for acute bacterial infection in swine a possible role for serum interleukin 6 . ^^^ Serum samples collected 0 , 10 , 20 , 28 and 44 h , and 3 , 4 , 7 , 13 and 17 days post infection were analysed for their content of interferon ( IFN ) alpha , IFN gamma , tumor necrosis factor ( TNF ) alpha by immunoassays and interleukin 6 ( IL 6 ) by a bioassay . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Proinflammatory cytokines ( TNF alpha , IL 1alpha , IL 6 ) , but not IFN gamma or TGF beta , induced activation of HSF 1 DNA binding and hsp 70 expression in cultivated SFC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PTX suppresses the production of tumor necrosis factor alpha ( TNF alpha ) and T helper type 1 ( Th 1 ) cytokine , interferon gamma ( IFN gamma ) , whereas it increases the production of Th 2 cytokines , such as interleukin 4 ( IL 4 ) and IL 10 . ^^^ The results of enzyme linked immunospot ( ELISPOT ) assay of spleen cells of mice showed that the production of TNF alpha and IFN gamma was significantly inhibited ( TNF alpha and IFN gamma , p < 0 . 001 ) and IL 4 and IL 10 production was significantly increased ( IL 4 , P < 0 . 001 ; and IL 10 , P < 0 . 05 , respectively ) in the group of mice treated with PTX from days 2 to 12 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Experiments were performed to evaluate the suitability of using cultured heparinized ( `` whole ' ' ) blood for induction of tumor necrosis factor alpha ( TNF alpha ) and gamma interferon ( IFN gamma ) , two cytokines critical in AIDS pathogenesis . ^^^ The mean coefficient of variation observed in three weekly determinations was 0 . 47 for TNF alpha and ranged from 0 . 12 to 1 . 73 for IFN gamma . ^^^ These values indicate that sample sizes of 8 , 24 , and 29 subjects would be sufficient to detect twofold changes in LPS induced TNF alpha and in PHA and antigen induced IFN gamma respectively , if two baseline and two treatment determinations were obtained , and if the interpatient variability of changes in true levels from baseline to follow up is negligible compared to the variability in the three weekly measurements . ^^^ Measurement of LPS induced TNF alpha and mitogen or antigen induced IFN gamma can be performed simply and reproducibly in human immunodeficiency virus infected persons by the whole blood culture method . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , the concentration of IFN gamma in pleural effusion was high in tuberculous patients , and that of TNF alpha was high in tuberculous and parapneumonic pleural fluid , but both cytokines were low in malignant pleural fluid . ^^^ CONCLUSIONS : Our results indicate that simultaneous measurement of IFN gamma and TNF alpha in pleural effusion is a useful diagnostic tool for differentiating tuberculous pleurisy from parapneumonic and malignant pleurisy . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro studies with myelin basic protein ( MBP ) specific T cell lines revealed a synergistic suppressive effect of IFN beta1b and the phosphodiesterase inhibitor pentoxifylline ( PTX ) on proliferation and the production of tumor necrosis factor alpha ( TNF alpha ) , lymphotoxin ( LT ) , and interferon gamma ( IFN gamma ) . ^^^ Patients treated with IFN beta1b alone reported more side effects during the first 3 months of treatment and had upregulated TNF alpha as well as IFN gamma mRNA expression during the first month , which was not detected in patients receiving both drugs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using RT PCR , we demonstrate IL 10 in vitro down regulation of mRNA expression of iNOS , the cytokines involved in nitric oxide signal transduction initiation ( IFN gamma and TNF alpha ) , and other mononuclear phagocyte associate cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lipopolysaccharide ( LPS ) and interferon gamma ( IFN gamma ) stimulated release of nitrite , lactate dehydrogenase ( LDH ) , TNF alpha , IL 1 beta and IL 8 from rat peritoneal macrophages , all of which were significantly reduced by ITU . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In competitive PCR analysis , levels of IFN gamma and perforin in xenografts on days 10 and 13 post transplantation ( PT ) were higher than those in isografts ( rat to rat ) at the same stage , whereas the levels of TNF alpha , which was detected only on day 7 PT , were not significantly different between the two groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast to the effects of IFN gamma and TNF alpha which sensitize glioma cells to CD 95 antibody induced apoptosis in part by enhancing CD 95 expression , IFN alpha has no effect on CD 95 expression at the cell surface of LN 229 and T98G cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The DP 4 inhibitors suppress DNA synthesis and IFN gamma , IL 4 , and TNF alpha production of the antigen stimulated TCC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NE reduced cellular activation and altered the production of several HIV modulating cytokines : IL 10 and IFN gamma were markedly suppressed ; TNF alpha , IL 1beta , IL 2 , IL 4 , and IL 6 were mildly suppressed ; and levels of IL 12 were not significantly altered . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
BEAS 2B cells were transfected with a replication deficient adenoviral vector , expressing a mutated , nondegradable form of 1 kappa B alpha . 1 kappa B alpha overexpression specifically blocked NF kappa B translocation and inhibited mRNA accumulation and secretion of RANTES induced by RSV or TNF alpha plus IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lymphocyte subtyping was performed and whole blood cell cultures were used to measure the cytokine producing capacity for interleukin 1 ( IL 1 ) , interleukin 2 ( IL 2 ) , interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) and interferon alpha ( IFN alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Evaluation of cytokine levels in BMC supernatants showed that Der p extract significantly increased the release of granulocyte macrophage colony stimulating factor ( GM CSF ) , interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin ( IL ) 1 beta and IL 2 ( p < 0 . 001 , each comparison ) . ^^^ Salmeterol ( 10 ( 7 ) M ) or dexamethasone ( 10 ( 9 ) M ) induced a similar , statistically significant , downregulation of GM CSF release ( p < 0 . 05 , each comparison ) and induced a slight , not significant , decrease in the production of IFN gamma , TNF alpha , IL 1 beta and IL 2 ( p > 0 . 05 each comparison ) . ^^^ Interestingly , salmeterol ( 10 ( 7 ) M ) significantly enhanced the inhibitory activity of dexamethasone ( 10 ( 9 ) M ) on the release of GM CSF , TNF alpha , IL 1 beta and IL 2 ( p < 0 . 05 , each comparison ) but not of IFN gamma ( p > 0 . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Elevated serum levels of IFN gamma , IL 4 and TNF alpha / unelevated serum levels of IL 10 in patients with demyelinating diseases during the acute stage . ^^^ We investigated IFN gamma , IL 4 , IL 10 and TNF alpha in the sera of 42 patients with demyelinating diseases : multiple sclerosis ( MS ) ( 21 ) , Guillain Barr syndrome ( GBS ) ( 14 ) , Miller Fisher syndrome ( MFS ) ( 7 ) during the acute or active stage and 12 normal controls using an originally devised sensitive sandwich enzyme linked immunosorbent assay ( ELISA ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In order to promote bradyzoite tachyzoite conversion , mice were treated in vivo with neutralizing doses of anti gamma interferon ( IFN gamma ) or anti tumor necrosis factor alpha ( TNF alpha ) antibodies . ^^^ The immunosuppressive effect of anti IFN gamma or anti TNF alpha treatment was immediate , leading to parasite stage conversion as indicated by the increased expression of tachyzoite specific antigens ( SAG 1 and SAG 2 ) and by rapid parasite replication . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
High serum IFN gamma was coinciding with the declination of platelet counts , but TNF alpha was positively associated with the platelet count , while high IL 2 level was similar to the course of TPO expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma , tumor necrosis factor ( TNF ) alpha and IL 12 , and cytotoxic , e . g . perforin and Fas FasL , pathways to reject incompatible bone marrow cell ( BMC ) grafts . ^^^ To begin a dissection of these two major pathways , mice bearing deletional mutations of IFN gamma , TNF RI / II or perforin , or mice treated with mAb to IL 12 , IFN gamma or NK1 . 1 were irradiated and challenged with class 1 deficient BMC grafts , a system in which only NK cells are the effector cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The massive influx of lymphocytes at day 14 was also accompanied by the synthesis of mRNA encoding IL1beta , TNFalpha , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Levels of pulmonary interleukin 6 ( IL 6 ) , tumor necrosis factor alpha ( TNF alpha ) , granulocyte colony stimulating factor ( G CSF ) , IL 1 receptor antagonist ( IL 1ra ) , and IL 10 mRNA , as well as hepatic IL 1beta , IL 6 , gamma interferon ( IFN gamma ) , G CSF , inducible nitric oxide synthase , and IL 10 mRNA , were reduced in MPL pretreated mice after CLP compared to control mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Correlation studies between cytokines expressed in islets and autoimmune diabetes development in NOD mice and BB rats have demonstrated that beta cell destructive insulitis is associated with increased expression of proinflammatory cytokines ( IL 1 , TNF alpha , and IFN alpha ) and type 1 cytokines ( IFN gamma , TNF beta , IL 2 and IL 12 ) , whereas non destructive ( benign ) insulitis is associated with increased expression of type 2 cytokines ( IL 4 and IL 10 ) and the type 3 cytokine ( TGF beta ) . ^^^ Cytokines ( IL 1 , TNF alpha , TNF beta and IFN gamma ) may be directly cytotoxic to beta cells by inducing nitric oxide and oxygen free radicals in the beta cells . ^^^ In addition , cytokines may sensitize beta cells to T cell mediated cytotoxicity in vivo by upregulating MHC class 1 expression on the beta cells ( an action of IFN gamma ) , and inducing Fas ( CD 95 ) expression on beta cells ( actions of IL 1 , and possibly TNF alpha and IFN gamma ) . ^^^ Transgenic expression of cytokines in beta cells of non diabetes prone mice and NOD mice has suggested pathogenic roles for IFN alpha , IFN gamma , IL 2 and IL 10 in insulin dependent diabetes mellitus ( IDDM ) development , and protective roles for IL 4 , IL 6 and TNF alpha . ^^^ In contrast , post natal deletions of IL 12 and IFN gamma , also IL 1 , TNF alpha , IL 2 , and IL 6 by systemic administrations of neutralizing antibodies , soluble receptors and receptor antagonists , and receptor targeted cytotoxic drugs significantly decrease IDDM incidence in NOD mice and / or BB rats . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 2 , IFN gamma , and tumor necrosis factor alpha mRNA were not detected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , although p 100 is highly expressed in a number of breast cancer cell lines , MHC Class 1 antigen expression was observed on all the cell lines we analysed and could be further induced by stimulation with the cytokines IFN gamma or TNF alpha . ^^^ Stable transfection of a unresponsive mutated IkappaB alpha Ser 32 36 expression vector showed that TNF alpha induced MHC Cl 1 expression in an NF kappaB dependent way while IFN gamma did it independently of any NF kappaB activation . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
During disease progression , large numbers of IL 4 , TNF alpha , and IFN gamma producing CD4+ and CD8+ cells accumulated within the intraepithelial spaces and lamina propria of the colon of IL 2 / mice . ^^^ Although colonic EC expressed receptors for IFN gamma and TNF alpha , these cytokines did not adversely affect EC viability or growth in vitro consistent with these cytokines not being the primary mediators of EC injury in IBD . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha is synergistic with IFN gamma for the induction of IP 10 . ^^^ We therefore decided to define the presence of tax , IFN gamma , TNF alpha , and IP 10 in lesions of 19 adults with classical CTCL who were seronegative for HTLV 1 . ^^^ Lesional mRNAs for actin , TNF alpha , IFN gamma , and tax were detected by reverse transcriptase polymerase chain reaction ( RT PCR ) amplification . ^^^ In addition IP 10 , TNF alpha , and IFN gamma were detected and localized with immunocytochemistry of frozen sections . ^^^ By RT PCR , mRNA for IFN gamma was detected in lesions of 8 , and for TNF alpha in lesions of 13 patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Enzymic activity was increased in response to inflammatory cytokines ; increases of 2 2 . 5 fold being seen with TNF alpha and IL 1beta and of approximately 8 fold with IFN gamma . ^^^ A combination of IFN gamma and TNF alpha , or of these two cytokines plus IL 1beta , led to responses representing the sum of those obtained by using the individual cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Also , treatment of infected mice with mAb specific for IFN gamma or TNF alpha inhibited the protective effect of exogenous IL 12 . ^^^ Therefore , while IL 12 , TNF alpha and IFN gamma correlate with resistance to T . cruzi infection , TGF beta and IL 10 promote susceptibility . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This review focuses on the role of IL 10 , TNF alpha , IFN gamma and IL 12 in controlling T . cruzi replication by the innate and specific immune systems of the vertebrate host . ^^^ A study performed on mice with disrupted recombinase activating genes ( RAG / KO ) , which lack T and B lymphocytes , revealed the importance of IL 12 , IFN gamma and TNF alpha in the resistance against T . cruzi mediated by the innate immune system . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The bulk of evidence points to a significant production of IFN gamma and TNF alpha which may be linked to T . cruzi induced IL 12 production . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Later during the disease , high levels of IFN gamma and TNF alpha are produced both in cutaneous and mucosal leishmaniasis . ^^^ Although production of IFN gamma and TNF alpha might be involved in the control of parasite multiplication in the early phases of Leishmania infection , these cytokines might also be involved in the tissue damage seen in tegumentary leishmaniasis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of IL 12 , IFN gamma , and TNF alpha correlated with disease activity and severity , while TGF beta was the only factor that was up regulated during the recovery phase . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In fibroblast primary cell cultures , CLF 1 mRNA was up regulated by TNF alpha , IL 6 , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Control of IL 12 and IFN gamma production in response to live or dead bacteria by TNF and other factors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Addition of CD 86 MAb to cocultures inhibited most p 24 ( 84 + / 12 % , n = 13 ) and IL 2 ( 99 + / 2 % , n = 6 ) production , decreased T cell proliferation by 46 + / 15 % ( n = 13 ) , and decreased TNF alpha and IFN gamma production by 67 + / 17 % ( n = 6 ) and 53 + / 6 % ( n = 6 ) , respectively . ^^^ In contrast , CD 80 MAb , which strongly inhibited IL 2 production ( 77 + / 10 % , n = 6 ) , moderately downregulated p 24 and TNF alpha production ( 29 + / 21 % , n = 13 and 34 + / 10 % , n = 6 , respectively ) and did not decrease T cell proliferation ( 8 + / 10 % , n = 13 ) or IFN gamma production ( 14 + / 13 % , n = 6 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In order to evaluate the roles of interferon gamma ( IFN gamma ) and tumor necrosis factor ( TNF alpha ) in the induction of NO synthesis in murine peritonitis , mice deficient in the respective cytokine receptors were studied . ^^^ In control in vitro experiments , macrophages from IFN gammaR or TNFR 55 deficient mice , while failing to respond to IFN gamma or TNF alpha , respectively , produced high levels of NO under appropriate stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here we investigated in mice the effect of this compound on the plasma tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , interleukin 1alpha ( IL 1alpha ) , interleukin 6 ( IL 6 ) , interleukin 10 ( IL 10 ) , interleukin 12 ( IL 12 ) , macrophage inflammatory protein 1alpha ( MIP 1alpha ) , and nitric oxide ( NO ) response to intraperitoneal ( i . p . ) injection of LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There was no correlation of MBP mediated lysis of astrocytes with TCR Vbeta usage , HLA restriction and the production of tumor necrosis factor alpha ( TNF alpha ) , lymphotoxin ( LT ) and interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Bacterial lipopolysaccharide ( LPS ) induced increases in serum cytokines ( TNF alpha , IL 1 and IL 6 ) and hypothermia were studied in mice sensitized by carrageenan pretreatment and compared with mice sensitized with heat killed P . acnes or IFN gamma , all given IP at appropriate intervals ( 24 hr , 7 days and 12 18 hr , respectively ) before LPS . 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The highest tier included prostaglandin E 2 ( PGE 2 ) , interleukin 1 beta ( IL 1 beta ) and interleukin 2 ( IL 2 ) , the intermediate tier included tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) and at the lowest concentration level were interleukin 4 ( IL 4 ) and interleukin 6 ( IL 6 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 2 ( IL 2 ) , interleukin 4 ( IL 4 ) , and interleukin 10 ( IL 10 ) were detectable in EAU eyes at near peak inflammation , with IFN gamma achieving the highest mean concentration ( 331 pg / ml ) . ^^^ In eyes from rats immunized with adjuvant only and in normal eyes , IFN gamma , TNF alpha , and IL 10 were nondetectable . ^^^ Thus , a direct method of measuring intraocular cytokine concentrations was successfully applied to reveal an elevation of IFN gamma , TNF alpha , IL 2 , IL 4 , and IL 10 in EAU eyes from rats immunized with IRBP , compared to rats immunized with adjuvant alone and to normal rats . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A so called Th 1 response , with an increased production of IFN gamma , TNF alpha and other pro inflammatory cytokines , occurs in the stomach when infected by Helicobacter pylori , in the small intestine when the subject with coeliac disease consumes normal bread and during the active phases of Crohn ' s disease . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Fetal lung explants were used to study the regulation of the SP A genes , SP A 1 and SP A 2 , by dexamethasone , interferon , gamma ( IFN gamma ) , cyclic 3 ' , 5 ' adenosine monophosphate ( cAMP ) , and tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of human islets with a combination of tumor necrosis factor ( TNF ) + lipopolysaccharide ( LPS ) + interferon gamma ( IFN gamma ) stimulates inducible nitric oxide synthase ( iNOS ) expression , nitric oxide production , and inhibits glucose stimulated insulin secretion . ^^^ The IL 1 receptor antagonist protein ( IRAP ) prevents TNF + LPS + IFN gamma induced iNOS expression and nitrite production , and attenuates the inhibitory effects on glucose stimulated insulin secretion by human islets . ^^^ Inhibition of iNOS activity by aminoguanidine also attenuates TNF + LPS + IFN gamma induced inhibition of insulin secretion by human islets . ^^^ These results indicate that the inhibitory effects of TNF + LPS + IFN gamma are mediated by nitric oxide , produced by the actions of IL 1 released endogenously within human islets . ^^^ Reverse transcriptase polymerase chain reaction was used to confirm that TNF + LPS + IFN gamma stimulates the expression of both IL 1alpha and IL 1beta in human islets . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma as well as TNF alpha , however , induced a significant , dose and time dependent increase in CD 95 mRNA and cell surface expression as measured by reverse transcription PCR and flow cytometry . ^^^ Co stimulation with IFN gamma and TNF alpha had synergistic effects on the CD 95 surface expression on eosinophils . ^^^ Addition of IL 3 , IL 5 or GM CSF to IFN gamma and TNF alpha stimulated eosinophils caused in a reduction of CD 95 expression . ^^^ Functional activity for CD 95 following incubation with IFN gamma and TNF alpha was demonstrated by increased apoptosis in response to cross linking with FasL . ^^^ From these data , we conclude that IFN gamma and TNF alpha can up regulate cell surface expression of CD 95 on eosinophils , which leads to an increased susceptibility of eosinophils to Fas mediated apoptosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vivo stimulation with anti CD 3 antibody in arthritic IFN gammaR KO mice resulted in production of higher levels of circulating IFN gamma , TNF and IL 2 than in corresponding control mice that had not received the arthritis inducing immunization . ^^^ Furthermore , the protective effect of anti IL 12 antibody in mutant , but not in wild type mice , was associated with lower circulating IFN gamma , TNF and IL 2 and higher IL 4 and IL 5 cytokine levels following an anti CD 3 challenge . ^^^ Furthermore , our study suggests that , in the absence of a functional IFN gamma system , endogenous IL 12 exerts its disease promoting effect by favoring production of other Th 1 associated cytokines ( IL 2 and TNF ) , by inhibiting development of IL 4 and IL 5 producing T cells and by stimulating production of anti collagen autoantibodies . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Leukocytes from tumor tissues ( TIL , n = 17 ) , axillary lymph nodes ( LNL , n = 26 ) , and peripheral blood ( PBL , n = 25 ) of human breast cancer patients were evaluated for the ability to synthesize type 1 cytokines ( TNF alpha , IFN gamma , and IL 2 ) and type 2 cytokines ( IL 4 , IL 6 , and IL 10 ) by flow cytometry . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CONCLUSIONS : Incubation with LPS associated with IFN gamma and TNF alpha induces alterations of RPE tight junctions , whereas NO is involved in the maintenance of their integrity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Fas expression was induced by culture in the presence of IFN gamma + tumor necrosis factor alpha ; however , the maximal level attained in Ras transformants was approximately 10 fold below the level of untransformed cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Induction of nitric oxide synthase ( NOS 2 ) expression in C 6 hsp70 cells , assessed by nitrite accumulation , was significantly reduced compared to control C 6 pTK cells ( 25+ / 8 % of control cell induction , P < 0 . 005 ) , when induced with a maximally stimulatory combination of bacterial endotoxin lipopolysaccharide ( LPS ) plus a mixture of three cytokines ( `` CM : ' ' TNF alpha , IL 1 beta , and IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma ( 50 100 ng / ml ) induced TNF alpha production dose dependently , but no induction of TNF alpha was observed with IFN alpha or IFN beta . ^^^ Since in the epidermis cytokines often work with in a cascade fashion and keratinocytes are a source of primary cytokine , IL 1 alpha , whether combined treatment with IFN gamma and IL 1 alpha had a synergistic effect on TNF alpha production was examined . ^^^ Combined treatment with IFN gamma ( 100 ng / ml ) and IL 1 alpha ( 10 ng / ml ) induced 2 3 fold higher level of TNF alpha than IL 1 alpha alone . ^^^ These results suggest that IFN gamma is a positive regulator for the production of TNF alpha from human keratinocytes and likely to increase skin inflammation . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A mice were studied regarding antigen elicited secretion of monokines ( TNF alpha and TGF beta ) and type 1 ( IL 2 and IFN gamma ) and type 2 ( IL 4 , 5 , 10 ) cytokines . ^^^ Taken together , these results suggest that an early secretion of high levels of TNF alpha and IFN gamma followed by a sustained secretion of IL 2 and IFN gamma plays a dominant role in the resistance mechanisms to P . brasiliensis infection . ^^^ In contrast , an early and ephemeral secretion of low levels of TNF alpha and IFN gamma associated with production of IL 5 , IL 10 and TGF beta characterizes the progressive disease of susceptible animals . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Proteins belonging to the NFAT ( nuclear factor of activated T cells ) family of transcription factors are expressed in most immune cell types , and play a central role in the transcription of cytokine genes , such as IL 2 , IL 4 , IL 5 , IL 13 , IFN gamma , TNF alpha , and GM CSF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Promoter activity was evaluated by transient transfection of hiNOS luciferase constructs in A 549 human alveolar type 2 epithelium like cells in the presence and absence of cytokines ( IFN gamma , IL 1 beta , and TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The phosphodiesterase i . v . inhibitor rolipram in vitro reduces the numbers of MBP reactive IFN gamma and TNF alpha mRNA expressing blood mononuclear cells in patients with multiple sclerosis . ^^^ In situ hybridization with synthetic oligonucleotide probes was used to detect and enumerate blood MNC expressing IFN gamma , TNF alpha , LT , TGF beta , IL 4 , and IL 10 mRNA . ^^^ Rolipram reduced the numbers of MBP reactive IFN gamma and TNF alpha mRNA expressing blood MNC in MS , and numbers of AChR reactive IFN gamma , TNF alpha , and LT mRNA positive cells in MG . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Also , i . pl . administration of LcS has been shown to induce the production of several cytokines , such as IFN gamma , IL 1beta and TNF alpha , in the thoracic cavity of mice , resulting in the inhibition of tumour growth and increased survival . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Superexpression was found with the combination of IFN gamma and TNF alpha or IL 1 , although these latter cytokines by themselves did not induce accumulation of IP 10 mRNA . ^^^ Nuclear run on experiments performed to investigate the regulation of IP 10 mRNA expression , showed a very high constitutive transcriptional activity of the IP 10 gene in unstimulated keratinocytes , which was not affected by stimulation with IFN gamma , TNF alpha , or a combination of IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Upon exposure to 2 and 5 mg / kg body wt VT , the relative abundance of IL 1beta , IL 6 , TNF alpha , IL 12 p 35 , IL 12p40 , IL 2 , and IL 10 mRNAs increased with dose frequency whereas IFN gamma and IL 4 mRNAs were unaffected . ^^^ When mice were fed 0 , 10 , and 25 ppm VT for 4 weeks , increased expression of mRNAs for TNF alpha , IL 2 , IFN gamma , and IL 10 was most prominent . ^^^ However , when VT fed mice were also challenged with an oral dose of VT equivalent to daily intake at 2 h prior to RNA isolation , vigorous mRNA responses were observed for IL 1beta , IL 6 , TNF alpha , IL 12p40 , IL 12p35 , IL 2 , IFN gamma , IL 4 , and IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Similarly , no differences in the levels of IL 2 , IL 4 , IL 10 , IFN gamma , GM CSF , or TNF alpha were found . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Macrophages stimulated with lipopolysaccharide ( LPS ) or heat killed Listeria monocytogenes in the presence or absence of IFN gamma produced minimal IL 12 p 70 by comparison with DC or monocytes , despite comparable production of TNF alpha . ^^^ Exposure of Mphi to TNF alpha , granulocyte macrophage CSF or IFN gamma did not substantially up regulate IL 12 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Subjective and autonomic responses were monitored , and blood was sampled at baseline , 5 , 20 , and 60 minutes after the stressor to assess mitogen stimulated production of interleukin 1beta ( IL 1beta ) , interleukin 4 ( IL 4 ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The dysregulation of cytokine gene expression , including interleukin 12 , tumor necrosis factor alpha , and gamma interferon ( IFN gamma ) , following human retrovirus infection is well documented . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , strong CD 28 costimulation supports the production of high levels of type 1 ( IL 2 , IFN gamma and TNF beta ) and low levels of type 2 ( IL 4 and IL 13 ) cytokines by neonatal T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Assay of culture supernatants by ELISA showed inhibition of TNF alpha , IFN gamma , IL 2 , and IL 4 production , which , for IL 2 and TNF alpha was also confirmed by intracellular cytokine staining . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Uncommitted rat bone marrow derived Mphi ( BMDMs ) were incubated with IFN gamma , TNF alpha , TGF beta , IL 4 , IL 6 , and IL 10 alone or sequentially in combinations . ^^^ IFN gamma followed 4 h later by TNF induced NO generation . ^^^ The pretreatment of BMDMs before IFN gamma priming with TNF , TGF beta , and IL 4 suppressed NO generation by 87 % , 92 % , and 85 % , respectively ; IL 10 had no effect . ^^^ The uptake of apoptotic polymorphonuclear leukocytes was augmented by TNF ( 40 % vs 29 % controls ; p < 0 . 05 ) and decreased by IFN gamma , IL 10 , and IL 4 . ^^^ The TNF response was unaffected by subsequent treatment with IFN gamma , IL 4 , or IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The present study analyzed the ability of tumor necrosis factor alpha ( TNF alpha ) , double stranded RNA , and interferon gamma ( IFN gamma ) to induce the transcription factor NF kappaB in astrocytes expressing or lacking SHP 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The objective of this study was to measure the circulating levels of IFN gamma , IL 10 and TNF alpha in patients with visceral leishmaniasis ( VL ) before , during and at the end of therapy and to examine the association between cytokine levels and activity of VL . ^^^ At baseline , we detected circulating levels of IFN gamma in 13 of 15 patients ( median = 60 pg / ml ) ; IL 10 in 14 of 15 patients ( median = 141 . 4 pg / ml ) ; and TNF alpha in 13 of 14 patients ( median = 38 . 9 pg / ml ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We report that interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) synergistically induce P2X7R mRNA and functional responses in the human THP 1 monocytic cell line . ^^^ Induction was dose dependent , with maximal functional activity requiring 1000 units / mL IFN gamma and 10 ng / mL TNF alpha and incubations of 36 72 h . ^^^ The up regulation of P2X7R function by lipopolysaccharide ( LPS ) / IFN gamma and TNF alpha / IFN gamma was markedly attenuated by coincubation with prostaglandin E 2 or the cell permeant cyclic AMP analog dibutryl cAMP ( Bt2cAMP ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Further stimulation for 72 120h resulted in a significant increase in the levels of IL 1beta , IFN gamma and tumour necrosis factor ( TNF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , we have studied the effects of IL 12 and IL 15 on induction of IFN gamma and TNF alpha production , NK , and LAK activities in CB and APB . ^^^ The impaired ability of CB to produce IL 12 and IL 15 in response to stimulation may contribute to the decrease in IFN gamma , TNF alpha production , NK and LAK activities . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Notably , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) are important mediators of the progress of liver injury . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Peripheral blood mononuclear cells ( PBMCs ) from 15 nonatopic subjects and 76 patients with perennial allergic rhinitis ( 18 untreated patients and 58 patients on immunotherapy ) were cultured in the absence and in the presence of a major Dermatophagoides farinae allergen , Der f 1 , and the levels of IgE , interleukin 5 ( IL 5 ) , interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) in the culture supernatants were determined . ^^^ The difference between the absence and presence of Der f 1 was calculated to consider the Der f 1 dependent synthesis of IgE , IL 5 , IFN gamma and TNF alpha . ^^^ The levels of Der f 1 dependent synthesis of IgE , IL 5 and TNF alpha were significantly higher in the untreated group than in the nonatopic group , whereas Der f 1 dependent synthesis of IFN gamma was significantly lower in the untreated group than in the nonatopic group . ^^^ Immunotherapy decreased the enhanced Der f 1 dependent synthesis of IgE , IL 5 and TNF alpha , and further decreased the suppressed Der f 1 dependent synthesis of IFN gamma as the therapy proceeded . ^^^ The levels of Der f 1 dependent synthesis of IgE and IL 5 , but not of IFN gamma and TNF alpha , were correlated significantly with the levels of symptom scores . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In response to cytokine stimulation ( tumor necrosis factor alpha , IFN gamma , and interleukin 1beta ) , human pancreatic carcinoma cell lines expressed the inducible NO synthase that synthesizes NO , detectable as nitrate and nitrite in the culture supernatants . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It was found that strain related susceptibility to diabetes induction correlated with a higher level of IL 2 , IFN gamma , and TNF alpha production , whereas such differences were not observed when IL 1 and NO production by macrophages were analyzed ; elimination of immunoregulatory RT6+T cells that increases IFN gamma production , enhances susceptibility to MLD STZ induced diabetes ; mercury induced Th 2 cells down regulated the disease ; IFN gamma mediated macrophage activation to produce proinflammatory cytokines rather than NO is an important event in early diabetogenic effects of invading macrophages ; inhibition of IL 1 activity downregulates diabetes induction ; and generation of NO in beta cells appears to be important for diabetogenic effects . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment with TNF alpha or IL 1 beta ( 10 ng / ml each ) induced an about 2 fold increase of IL 15 mRNA ; IFN gamma induced significant effects only at 100 ng / ml . ^^^ Stimulation with a combination of TNF alpha and IFN gamma was not superior to stimulation with TNF alpha alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although proliferation and RNA synthesis induced by PMA / anti Ig could be enhanced by the addition of IL 2 , IL 4 , IL 13 , IFN gamma or TNF alpha , the presence of these cytokines failed to abrogate the IL 10 mediated inhibition of leukemic CD5+ B cell activation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CONCLUSIONS : The results show that mucosal gluten exposure in patients with celiac disease rapidly elicits high levels of IFN gamma expression and lower levels of IL 2 , IL 4 , IL 6 , and tumor necrosis factor alpha even in the virtual absence of IL 12 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using a whole blood , cell culture system , we studied the effects on the release of tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and interleukin 6 ( IL 6 ) in six healthy subjects , and , in granulocyte suspensions , the effects on the release of reactive oxygen species ( ROS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Numerous studies indicate that proinflammatory substances like tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) as well as macrophage derived neopterin are increased in atherosclerotic tissue and thus are potentially involved in the process of atherogenesis . ^^^ Since apoptotic death of vascular smooth muscle cells ( VSMC ) is reported to occur in atherosclerotic lesions , we investigated the effects of neopterin , TNF alpha , and IFN gamma on apoptosis in cultured VSMC . ^^^ Morphological changes characteristic of apoptosis as well as DNA fragmentation were detected in cells treated with neopterin , TNF alpha / IFN gamma , and neopterin + TNF alpha / IFN gamma . ^^^ Simultaneously , neopterin , TNF alpha / IFN gamma , and neopterin + TNF alpha / IFN gamma led to inducible nitric oxide synthase ( iNOS ) gene expression and nitric oxide ( NO ) synthesis . ^^^ We conclude that neopterin as well as TNF alpha / IFN gamma are potent mediators of apoptotic death in VSMC which is at least in part triggered by NO synthesis induced by these proinflammatory mediators . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The mRNAs of interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) were induced in all these organs . ^^^ At 24 hr after Con A treatment , the expression of IFN gamma mRNA , but not that of TNF alpha mRNA , was inhibited by cyclosporine A ( 50 mg / kg , i . p . ) , suggesting that Con A induced these cytokine mRNAs through different mechanisms . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using flow cytometry and a three step cellular ELISA method , we determined whether SP has an influence on the expression of MHC class 2 , intercellular adhesion molecule 1 ( ICAM 1 ) , VCAM 1 , LFA 3 , CD 40 , B7 . 1 or B7 . 2 molecules on RA FLS incubated with interferon gamma ( IFN gamma ) or IL 1beta or tumour necrosis factor alpha ( TNF alpha ) with or without SP . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Unstimulated LCM increased proliferation of MG 63 cells and this increase was not affected by neutralization of interleukin 1 ( IL 1 ) , IL 3 , IL 6 , granulocyte macrophage colony stimulating factor ( GM CSF ) , tumour necrosis factor ( TNF ) , lymphotoxin alpha , or interferon gamma ( IFN gamma ) . ^^^ The decrease in proliferation was abolished by neutralization of IFN gamma but was unaffected by neutralization of IL 1 , IL 2 , IL 3 , IL 4 , IL 6 , GM CSF , TNF , or lymphotoxin alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OBJECTIVE : PBMCs of diisocyanate exposed workers were tested in vitro for diisocyanate antigen specific enhancement of monocyte chemoattractant protein 1 ( MCP 1 ) , monocyte chemoattractant protein 3 ( MCP 3 ) , macrophage inflammatory protein 1alpha , RANTES , IL 8 , and T cell cytokines that could play a regulatory role in chemokine synthesis ( IL 4 , IL 5 , IFN gamma , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When the cells were treated with TNF alpha and IFN gamma in combination , RANTES production was enhanced , but IL 8 production was not changed , compared to TNF alpha treated cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OBJECTIVES : Multiple sclerosis ( MS ) is characterized by high levels of circulating mononuclear cells ( MNC ) that respond to myelin proteins like myelin basic protein ( MBP ) in vitro by expressing mRNA of both pro inflammatory cytokines , e . g . interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) and lymphotoxin ( LT ) that may make MS worse , and anti inflammatory cytokines like IL 4 , IL 10 and transforming growth factor beta ( TGF beta ) that may act beneficially . ^^^ MATERIAL AND METHODS : In situ hybridization to detect and enumerate IFN gamma , TNF alpha , LT , IL 4 , IL 10 and TGF beta mRNA expressing blood MNC after stimulation with myelin basic protein ( MBP ) , control antigens and without antigen in presence and absence of Linomide ( roquinimex , LS 2616 ) was employed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NO production by hepatocytes was not significantly enhanced by LPS , but was markedly enhanced by IL 1beta or the combination of tumor necrosis factor alpha ( TNF alpha ) and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Recombinant Mtb 8 . 4 Ag induced significant proliferation as well as production of IFN gamma , IL 10 , and TNF alpha , but not IL 5 , from human PBMC isolated from PPD positive healthy donors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
No differences in the lung levels of the specific cytokines TNF alpha , IFN gamma , IL 12 , and IL 10 were noted . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pretreatment of endothelial cells with IFN gamma for 24 to 72 h before 6 to 24 h activation with IL 1beta , TNF alpha , or PMA resulted in significantly reduced levels of cell surface E selectin , although levels of ICAM 1 and VCAM 1 were the same or increased . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of MIP 3 beta mRNA was not constitutive , but was inducible in bone marrow stromal cells by inflammatory agents such as bacterial LPS , IFN gamma , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF , possibly via induction of IL 6 , and IFN gamma induce hypoferraemia , which may in part result from a decrease in tissue iron release based on a primary stimulation of ferritin synthesis . ^^^ The fall in sTfR levels may reflect an impaired erythroid growth and / or TfR expression mediated by TNF and IFN gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro study using MBP specific T cells showed that the addition of T 614 inhibited the proliferative responses of T cells and the production of pro inflammatory cytokines such as IFN gamma , IL 6 and TNF produced by T and accessory cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
While IFN gamma alone induces NO release from microglia , astrocytes are provoked into significant NO production only by a combination of IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine gene expression of TNF alpha , IFN gamma , and IL 10 was routinely seen , but IL 4 expression was absent except in two clinically quiescent patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
INTERVENTIONS : Measurement of plasma concentrations of interleukin 1beta ( IL 1beta ) , interleukin 1 receptor antagonist ( IL 1ra ) , interferon 7 ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) were measured by enzyme linked immunosorbent assays ( ELISA ) . ^^^ Patients who survived had significantly higher IL 1beta concentrations than those who died ( 13 ( 1 ) compared with 3 ( 1 ) pg / ml , p = 0 . 005 ) CONCLUSION : There are significant time dependent changes in plasma concentrations of IL 1beta , IL 1ra , IFN gamma and TNF alpha after serious burns . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human umbilical vein EC ( HUVEC ) , dermal microvascular EC ( HMVEC ) , or the EC line EA . hy926 stimulated with gamma interferon ( IFN gamma ) or tumor necrosis factor alpha ( TNF alpha ) displayed upregulated HLA class 2 and adhesion molecule ( CD 54 and CD 106 ) expression , respectively . ^^^ SEA primed T cells induced a strong cytotoxicity against IFN gamma and TNF alpha activated EA . hy926 which had been preincubated with SEA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A preliminary evaluation was carried out with whole blood from two of the normal donors , and cells were stained immediately for HLA DR and CD11b markers or stained after incubation at room temperature or 37 degreesC for 18 h with or without the addition of the cytokines gamma interferon ( IFN gamma ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , IFN gamma plus GM CSF , tumor necrosis factor beta , or interleukin 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the differences between the lavage parameters including tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) release by lavage leukocytes in control rats and in animals intratracheally instilled with short and long amosite and wollastonite fibres . ^^^ To compare the harmful effects of these fibres , the number of leukocytes / ml of bronchoalveolar lavage ( BAL ) , the number of alveolar macrophages ( AM ) per ml of BAL , AM : granulocyte ( GR ) ratios in lavage fluid , phagocytic activity and viability of AM , lactate dehydrogenase ( LDH ) , acid phosphatase ( AcP ) , and TNF alpha and IFN gamma release by lavage leukocytes were investigated 3 months after the first intratracheal instillation . ^^^ Long amosite fibres significantly decreased the numbers of AM / ml BAL , increased the number of granulocytes depressed the phagocytic activity and viability of AM , and significantly decreased the levels of TNF alpha and IFN gamma in supernatants of cultured leukocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha and TGF beta 1 inhibited the IFN gamma induced MHC class 2 expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The serum concentrations of interleukin 6 ( IL 6 ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) were measured by enzyme immunoassays in 44 patients with Chlamydia ( n = 13 ) or Mycoplasma ( n = 14 ) pneumonia or influenza A infection ( n = 17 ) and in 20 control subjects . ^^^ IFN gamma , IL 6 and TNF alpha are present in the circulation in the majority of patients with Chlamydia and Mycoplasma pneumonia and in influenza A infection . ^^^ We suggest that repeated measurement of cytokines , such as IL 6 , IFN gamma and TNF alpha , may be useful in the management of lower respiratory tract infections but further studies are needed to define the value of cytokine measurements in acute pneumonia . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To investigate the role in vitro of CD 18 and ICAM 1 , we measured adhesion of fluorescently labeled human eosinophils to normal human bronchial epithelial cell ( NHBEC ) monolayers pretreated for 24 h with culture medium ( low constitutive ICAM 1 ) or tumor necrosis factor alpha ( TNF alpha ; 1 ng / ml ) and interferon gamma ( IFN gamma ) ( 10 ng / ml ; increased ICAM 1 ) . ^^^ In contrast , C5a and PMA induced adhesion to TNF alpha / IFN gamma activated NHBEC ( increased from 11 . 1 + / 1 . 3 % to 21 . 9 + / 1 . 0 % and 27 . 6 + / 1 . 9 % , respectively ) was CD 18 and ICAM 1 dependent . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We conclude that cell membrane DAF expression in the studied human lung cancer cell lines is modulated by IL 1alpha , TNF alpha , and IFN gamma , and speculate that cytokine mediated modulation of cell membrane DAF in human lung cancer cells might affect lung cancer cell biology . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study , we analyzed the effect of IFN gamma and TNF alpha on both the expression of HLA DR and ICAM 1 molecules on HGCN ( Germa 2 ) , and on their susceptibility to lysis by LAK cells . ^^^ Our results show that 1 , 000 U / ml IFN gamma induced a substantial increase in the expression of both ICAM 1 molecules and HLA DR on the cell surface , while the effect of TNF alpha on the expression of these molecules was substantially less prominent . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The production of endogenous interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) , which are critical in antilisterial resistance , was reduced in the aly / aly mice during the infection . ^^^ These results suggest that acquired immunity against L . monocytogenes infection is attenuated in aly / aly mice , and that the insufficient production of IFN gamma and TNF alpha might be involved in the immunodeficiency . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The enhanced CTL results are supported by the increased expression of Th 1 cytokines IFN gamma and TNF alpha and the reduction of IgG1 / IgG2a ratio . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Freshly prepared amalgam as well as ACCM induced a decrease in the production of interferon gamma ( IFN gamma ) and interleukin 10 ( IL 10 ) , and an increase in the concentrations of tumor necrosis factor alpha ( TNF alpha ) . ^^^ Cu2+ and Hg2+ induced a decrease in IFN gamma and IL 10 levels , and Hg2+ an increase in TNF alpha concentrations . ^^^ However , Ag+ markedly suppressed the production of IFN gamma , IL 10 , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Further analysis included histologic examination of the distal colon for colitis , staining of CD4+ T cells for surface activation markers , and cytoplasmic staining of CD4+ T cells for IFN gamma and TNF alpha . ^^^ Cytoplasmic staining revealed IFN gamma production , but not TNF alpha production by CD4+ T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We demonstrate that CD 40 is expressed on cultured human ASM and that expression can be increased by treatment with TNF alpha or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Alendronate treatment of peripheral blood mononuclear cells also resulted in an increased production of interleukin 1beta ( IL 1beta ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After stimulation with antigen or antigen + IL 12 , increased proliferation and production of interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) were observed in 23 of the 33 patients . ^^^ Lower baseline IL 12 and LPS induced IFN gamma , TNF alpha , and IL 12 production was observed in group 2 due to a possible dysfunction of accessory cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The percentage of cells that expressed IL 2 , IFN gamma , and TNF alpha was significantly increased in HSV 1 infected CD3+ , CD4+ , CD8+ , CD45RA+ , and CD45RO+ lymphocytes compared with uninfected lymphocytes from adult PBMC . ^^^ These results demonstrate that HSV 1 enhances intracellular levels of IL 2 , IFN gamma , and TNF alpha in adult lymphocytes and defective IFN gamma production in cord blood . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
At 90 min after immunization , mice immunized with antibiotic treated E . coli showed tumor necrosis factor alpha ( TNF alpha ) levels significantly lower and interleukin ( IL ) 6 levels significantly higher ( P < 0 . 05 ) than mice immunized with untreated E . coli , while comparable levels of interferon gamma ( IFN gamma were measured in both groups . ^^^ These data showed that in the presence of a protective immune response the survival of immunized mice was correlated with an early alteration of cytokine expression pattern including enhanced secretion of IL 4 , IL 6 and IFN gamma , and reduced secretion of TNF alpha and IL 10 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation of these cells with either TNF alpha , IL 1beta or IFN gamma induce further release of RANTES in a dose dependent manner with TNF alpha being the most potent stimulus . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Live H . pylori induced production of many cytokines , such as IL 1beta , IL 10 , IL 8 , IFN gamma , and TNF alpha , whereas we could not detect IL 2 or IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast to IFN gamma , other cytokines [ interleukin ( IL ) 1beta , IL 6 and tumor necrosis factor ( TNF ) alpha ] that can be found in brain areas affected by AD , did not stimulate C 1 Inh secretion by astrocytes or neuroblastomas in vitro . ^^^ Since IFN gamma is apparently lacking in the brain parenchyma , and amyloid plaque associated cytokines ( IL 1beta , IL 6 , TNF alpha ) do not stimulate C 1 Inh expression in vitro , the nature of the stimulus responsible for neuronal C 1 Inh expression in AD brains remains to be investigated . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
B . firmus stimulated in a concentration and time dependent manner the secretion of tumour necrosis factor alpha ( TNF alpha ) and interleukin 10 ( IL 10 ) , but it had no influence upon interferon gamma ( IFN gamma ) and interleukin 2 ( IL 2 ) production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The production and release of pro inflammatory cytokines are affected by theophylline showing a potent inhibitory effect on the production of IL 1beta , TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we examined the effect of gamma interferon ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 6 ( IL 6 ) , and IL 1 on the growth of T . gondii in a primary murine astrocyte culture . ^^^ IFN gamma in combination with either TNF alpha , IL 1 , or IL 6 caused a 75 to 80 % inhibition of growth . ^^^ Furthermore , IFN gamma in combination with IL 1 , IL 6 , or TNF alpha also induced inhibition in astrocytes derived from syngeneic mice deficient in the enzyme inducible nitric oxide synthase . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These in vitro responses correlated with increased IFN gamma and tumor necrosis factor alpha ( TNF alpha ) in the BAL fluid . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Likewise , peritoneal cells from both OK 432 and LPS administered mice were hyporesponsive to LPS , serum , TNF alpha , IFN gamma , and PMA in terms of IL 6 production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Synergistic stimulation of MHC class 1 and IRF 1 gene expression by IFN gamma and TNF alpha in oligodendrocytes . ^^^ In order to understand the molecular basis of the synergistic action of interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) on rat oligodendrocyte development , we studied some aspects of the signalling pathways involved in the regulation of the major histocompatibility complex ( MHC ) class 1 and the interferon regulatory factor 1 ( IRF 1 ) gene expression . ^^^ Neither IFN gamma nor TNF alpha was capable of inducing MHC CRE binding activity when administrated alone . ^^^ With respect to ICS element , IFN gamma induced IRF 1 binding , that was further enhanced upon co treatment with TNF alpha . ^^^ The existence of a synergism between IFN gamma and TNF alpha in stimulating IRF 1 expression at the transcriptional level was supported by IRF 1 promoter analysis : IFN gamma directly induced the binding of STAT 1 homodimers to the GAS element , while NF kappaB binding to the kappaB sequence was activated by TNF alpha only after IFN gamma treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Results show that SIVmac 251 establishes a persistent infection in primary astroglial cultures and that viral replication can be reactivated by TNF alpha , GM CSF , IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines , especially IL 2 , IL 4 , IL 6 , TNF , and IFN gamma , were heavily expressed during the acute phase of arthritis in cellular infiltrate . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
T cells from IL 10 ( / ) mice exhibit a stronger Ag specific proliferation , produce more proinflammatory cytokines ( IFN gamma and TNF alpha ) when stimulated with an encephalitogenic peptide , and induce very severe EAE upon transfer into wild type mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Monocytes were incubated with control medium , IFN gamma , TNF alpha , and calcitriol , a combination permissive to M . tuberculosis growth , or IFN gamma and IL 3 and infected with a low inoculum of M . tuberculosis ( Erdman ) . ^^^ IFN gamma / IL 3 treatment reduced M . tuberculosis CFU relative to both untreated and IFN gamma / TNF alpha / calcitriol treated monocytes . ^^^ Specifically , CFU were reduced by 79 % at 14 days in the IFN gamma / IL 3 treatment group relative to the IFN gamma / TNF alpha / calcitriol treatment group , an effect that was not due to toxic monocyte metabolites . ^^^ In contrast , monocyte monolayers treated with IFN gamma / TNF alpha / calcitriol consisted of a syncitium of cells containing monocyte aggregates . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Production of gamma interferon ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) in Trypanosoma cruzi infected mice results in the activation of inducible nitric oxide synthase ( iNOS ) and in elevated nitric oxide ( NO ) synthesis , which is important for the macrophage trypanocidal activity . ^^^ Inhibition of NO production by the addition of the L arginine analog NG monomethyl L arginine ( L NMMA ) , or of monoclonal antibodies ( mAbs ) to IFN gamma or TNF alpha spleen cells , partially restored cell viability and caused a decrease in the levels of apoptosis in splenocytes from infected animals . ^^^ Treatment of infected mice with L NMMA , anti TNF alpha , or anti IFN gamma mAbs caused reduction of both NO production and the amount of apoptotic cells , suggesting that NO plays a direct role in the induction of apoptosis in vivo . ^^^ Taken together , these data support the hypothesis that , as well as modulating immunosuppression , NO produced by IFN gamma and TNF alpha activated macrophages plays a role in apoptosis induction during the acute phase of experimental T . cruzi infection . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pentoxifyllin ( Ptx ) regulates the production of several cytokines including tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 ( IL 1 ) and , interferon gamma ( IFN gamma ) . ^^^ DESIGN : REF derived from extraocular muscles of healthy subjects were cultured without and with cytokines ( IFN gamma , TNF alpha and IL 1 ) and the effect of Ptx on the production of GAG by REF and HLA DR expression was determined . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) and interleukin 2 ( IL 2 ) production , serum neopterin levels , and T lymphocyte subpopulations were determined on a monthly basis in 22 MS patients . ^^^ There was a significant correlation with in vitro IFN gamma release , the absolute blood monocyte count and the serum neopterin levels , suggesting that monocytes stimulated by IFN gamma play an important role in the TNF alpha production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of TNF alpha and TNF beta mRNA was observed in samples from patients but not controls , while no differences were observed for mRNA of classical Th 1 cytokines ( IL 2 and IFN gamma ) between patients before or during treatment as well as controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In 76 HIV+ patients at different disease stages and in 25 controls we measured cytokine ( TNF alpha / beta , interferon gamma ( IFN gamma ) , IL 2 , IL 4 , IL 10 ) , and activation marker secretion ( sCD 4 , sCD 8 , sCD 30 ) in phytohaemagglutinin ( PHA ) stimulated and unstimulated PBMC cultures by ELISA , and membrane bound TNF alpha and CD 30 expression by flow cytometry . ^^^ We found an expansion of the TNF system in HIV+ individuals , that positively correlated with TNF alpha , IFN gamma and sCD 8 , probably representing activation of the cytotoxic compartment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using immunomagnetic separation techniques , we further demonstrate that the patients ' CD57+ / CD28 T cells display a typical Th 1 type cytokine secretion profile upon anti CD 3 stimulation , with a markedly higher secretion of the Th 1 type cytokines IL 2 , IFN gamma , and TNF alpha than their CD 57 / CD28+ counterparts . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Ionizing radiation potentiates the induction of nitric oxide synthase by IFN gamma and / or LPS in murine macrophage cell lines : role of TNF alpha . ^^^ Application of anti tumor necrosis factor alpha ( TNF alpha ) antibody before irradiation blocked induction of NO by IFN gamma . ^^^ We conclude ( 1 ) that irradiated cells produce more NO in response to either IFN gamma or LPS and ( 2 ) that the increase is mediated by induction of TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cells were cultured in the absence or presence of phytohaemagglutinin for 48 h , and total amounts of interleukin 2 ( IL 2 ) , IL 4 , IL 10 , IL 13 , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) were measured . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We now show that superantigen ( SAg ) , in combination with intracellular adhesion molecule 1 ( ICAM 1 ) costimulation drives a proliferative response accompanied by high levels of interleukin 2 ( IL 2 ) and moderate levels of interferon gamma ( IFN gamma ) and tumour necrosis factor ( TNF ) . ^^^ This response profile differs from that observed in B 7 or LFA 3 costimulated T cells because our previous results showed that B 7 CD28 costimulation was accompanied by high levels of IL 2 , IFN gamma and TNF , whereas LFA 3 was a potent inducer of IFN gamma and TNF , but had little influence on IL 2 production . ^^^ SAg induced IL 2 , IFN gamma and TNF were detected in both CD4+ and CD8+ T cells , whereas production of IL 10 was restricted to CD4+ T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In order to investigate the ability of rat peritoneal eosinophils to produce nitric oxide ( NO ) induced by cytokines in vitro , these cells were activated with several cytokines ( IL 5 , IL 8 , Rantes , TNF alpha , IFN gamma ) in association or not with LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pre treatment of SR 91 cells with TNF alpha or IFN gamma , increased both ICAM 1 ( CD 54 ) expression on SR 91 cells and binding to the human NK cell line NK 92 . ^^^ TNF alpha , but not IFN gamma also induced the activation of LFA 1 , CD 44 and beta 1 integrins on SR 91 cells . ^^^ Based on these observations we propose that the differential effect of TNF alpha and IFN gamma could be related to the activation of certain adhesion molecules on the surface of SR 91 cells by TNF alpha that , upon interaction with their counter receptors on NK 92 cells , lead to the activation of the NK 92 cells . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Fever , MT number in sputum , and abnormalities in HRCT images showed significantly earlier resolution in the IFN alpha treated group , together with a more significant decrease in BALF interleukin 1beta ( IL 1beta ) , IL 6 , and tumor necrosis factor alpha ( TNF alpha ) concentrations and significantly greater pre versus posttreatment variations in IL 2 and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Some of the actions induced by TNF alpha , and IFN gamma , including both beneficial and detrimental , are mediated by inducible nitric oxide synthase ( iNOS ) derived nitric oxide ( NO ) production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Sphingomyelin hydrolysis is induced in myeloid cell lines by tumour necrosis factor alpha ( TNF alpha ) , interleukin 1 beta ( IL 1beta ) , and interferon gamma ( IFN gamma ) . ^^^ In the present study , the contribution of ASMase to TNF alpha , IL 1beta , and IFN gamma signalling in murine macrophages was addressed . ^^^ Specifically , TNF alpha and IFN gamma induced nitric oxide production and TNF alpha and IL 1beta induced expression of the alpha chemokine , KC , were intact in ASMase null macrophages . ^^^ Also normal in ASMase null macrophages was TNF alpha , IL 1beta and IFN gamma induced expression of a panel of early response genes . ^^^ It is concluded that ASMase is non essential for the inflammatory signals activated in murine macrophages by TNF alpha , IL 1beta and IFN gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the course of inflammatory demyelinating neuropathies , proinflammatory cytokines induce vascular permeability and breakdown of blood nerve barrier ( TNF alpha , vascular endothelial growth factor / vascular permeability factor VEGF / VPF ) , favor leukocyte transmigration into nerve , induce activation and proliferation of lymphocytes ( IL 1 , IL 2 ) and macrophages ( gamma interferon IFN gamma ) , and have a direct myelinotoxic activity ( TNF alpha and TNF beta ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Examination of the cytokines essential for the generation of HCMV permissive Allo MDM identified gamma interferon ( IFN gamma , but not IL 1 , IL 2 , tumor necrosis factor alpha , or GM CSF as critical components in the generation of these macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma , TNF alpha , IL 4 and IL 10 were evaluated in the PFMC supernatants stimulated by these antigens . ^^^ Both crude and 70 kDa antigens induced higher levels of IFN gamma , TNF alpha and IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , dexamethasone inhibited markedly the expression of all cytokines tested ( IL 2 , IL 4 , IL 6 , IL 10 , IFN gamma and TNF alpha ) in dose dependent fashion , reducing levels to near to background . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , CoPP decreased the production of IL 10 , IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and lipopolysaccharide ( LPS ) , all proinflammatory factors , actually decreased ( IFN gamma and LPS ) , or had no effect ( TNF alpha ) on myelin phagocytosis by macrophages , but substantially increased phagocytic activity by microglia . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To determine the mechanism by which steroid treatment minimizes these side effects , we analyzed the percentage of interleukin 6 ( IL 6 ) , interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and IL 10 producing cells before and after 3 months of IFNbeta 1b therapy onset . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Effects of ribavirin on human and murine phytohaemagglutinin ( PHA ) activated T cells included inhibition of in vitro proliferation and modulation of IL 2 , IL 4 , IFN gamma and TNF alpha levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Certain sequences of nucleotides ( CpG motifs ) in bacterial DNA or synthetic oligonucleotides ( CpG DNA ) promote the production of proinflammatory cytokines , including TNF alpha , IFN gamma , IL 6 , and IL 12 . ^^^ CsA did not inhibit CpG DNA induced TNF alpha or IL 6 production , but decreased the production of IFN gamma by CpG DNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Renca cells normally express low levels of Fas that can be substantially enhanced by either IFN gamma or TNF alpha , and the combination of IFN gamma + TNF alpha synergistically enhances cell surface Fas expression . ^^^ In addition , cells pretreated with IFN gamma and TNF alpha are sensitive to lysis mediated by Fas ligand ( FasL ) expressing hybridomas ( dllS ) , cross linking of anti Fas Abs or soluble Fas ( FasL ) . ^^^ However , for the Fas mediated pathway to function , cell surface levels of Fas need to be increased beyond a critical threshold level by proinflammatory cytokines such as IFN gamma and TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma , IL 12 , and TNF alpha are required to maintain reduced liver pathology in mice vaccinated with Schistosoma mansoni eggs and IL 12 . ^^^ In this study , we demonstrate that in contrast with egg / IL 12 sensitized animals that showed marked decreases in pathology , mice similarly sensitized but depleted of IFN gamma , IL 12 , or TNF alpha at the time of egg laying developed granulomas that were similar to the non IL 12 treated control group . ^^^ Together , these data reveal key roles for IFN gamma , IL 12 , and TNF alpha in the protective effects mediated by this IL 12 based vaccine to prevent pathology . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The experiments showed that IL 12 , IL 18 , and TNF alpha , which were produced by chitin stimulated Mphi , contributed to the IFN gamma inducing activity of chitin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TMEV infected SJL / J mice expressed IFN gamma , TNF alpha , IL 10 , and IL 4 mRNA during the preclinical phase , and their levels continued to increase throughout the duration of the chronic progressive disease course . ^^^ In contrast , SJL / J mice with PLP ( 139 151 ) induced R EAE displayed a biphasic pattern of CNS expression for the proinflammatory cytokines , IFN gamma and TNF alpha , with expression peaking at the height of the acute phase and relapse ( s ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The roles of CD4+ T cells , IFN gamma and TNF alpha in viral clearance from the central nervous system ( CNS ) were examined in perforin gene deficient ( PKO ) mice . ^^^ PKO mice treated with anti IFN gamma mAb also had a significant 1 log 10 increase in infectious virus whereas inhibition of TNF alpha did not alter viral clearance or clinical disease in the PKO mice . ^^^ These data suggest , in addition to perforin mediated cytolysis , CD4+ T cells and IFN gamma , but not TNF alpha could contribute to JHMV clearance from the CNS . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , the level of transcriptional expression of the regulatory calcium binding S 100 proteins MRP 8 , MRP 14 and CP 10 was associated with inflammatory demyelination and expression of IFN gamma , IL 2 , TNF alpha , and iNOS . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We show here that experimental infection with a single bacterial agent , Helicobacter hepaticus , induces chronic colitis in SPF reared IL 10 ( / ) mice and that the disease is accompanied by a type 1 cytokine response ( gamma interferon [ IFN gamma ] , tumor necrosis factor alpha , and nitric oxide ) detected by restimulation of spleen and mesenteric lymph node cells with a soluble H . hepaticus antigen ( Ag ) preparation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Gamma interferon ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and interleukin 10 ( IL 10 ) production by liver , spleen , lung , peripheral blood mononuclear cells ( MNC ) , and peritoneal exudate cells ( PEC ) in experimental bacterial peritonitis was examined by cecum ligation and puncture ( CLP ) ( with an 18 gauge needle ) of BALB / c mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The same cytokines were expressed in the pregnant and nonpregnant uterine interplacentomal epithelium with detectable mRNA for interferon ( IFN ) gamma , tumor necrosis factor ( TNF ) alpha , and interleukin ( IL ) 1 alpha , but not for IL 2 or IL 4 . gamma delta TCR+ and CD8+ IEL purified from pregnant uteri expressed mRNA for IFN gamma , TNF alpha , transforming growth factor beta , and IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We also showed that after priming the cultures with IFN gamma , TNF alpha was unable to induce neuronal death unless oxygen free radicals were exogenously provided . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CD 40 expression was maximally induced by a combination of IFN gamma and IL 1 , with lower levels of induction observed with a mixture of IFN gamma and tumor necrosis factor ( TNF ) alpha or TNF alpha alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Culture of LP CD 34 ( + ) cells for 24 to 36 hours in the presence of tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) resulted in a significant increase in MIP 1alpha receptor expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Intracellular cytokine production was assayed by fluorescence activated cell sorter ( FACS ) , and the expanded T cell cultures produced IL 2 , IFN gamma , and tumor necrosis factor alpha ( TNF alpha ) , but not IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Because of its prominent expression in central nervous system inflammatory pathology by astrocytes , we examined the mechanism of human IP 10 ( hIP 10 ) gene induction by interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) in astrocytoma cells . ^^^ When present together , IFN gamma and TNF alpha induced robust accumulation of hIP 10 mRNA , but hIP 10 mRNA was minimally induced when astrocytoma cells were treated with individual cytokines . ^^^ Functional analysis of the hIP 10 promoter after deletion and substitution mutagenesis indicated that an interferon stimulated response element ( ISRE ) governed both simple response to IFN gamma and synergy with TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The reverse transcription polymerase chain reaction ( RT PCR ) was utilized to determine the mRNA expression of IFN gamma , TNF alpha , IL 2 , IL 4 , IL 6 and IL 10 . ^^^ CONCLUSIONS : Our study reveals an interesting correlation between the expression of TNF alpha , IFN gamma and disease progression in EAAU . ^^^ Furthermore , they suggest that TNF alpha is an important cytokine in the target tissue , while IFN gamma is in the draining lymph node . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Production of IL 2 , IL 6 , tumor necrosis factor alpha , or IFN gamma of the patient ' s PBMCs after appropriate stimulation was observed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have investigated the secretion of interferon alpha ( IFN alpha ) , IFN gamma , interleukin 1alpha ( IL 1alpha ) , IL 1beta , IL 2 and tumour necrosis factor alpha ( TNF alpha ) in whole blood cell cultures ( WBCCs ) of colorectal cancer patients upon mitogen stimulation . ^^^ Whereas the values for IL 1beta and TNF alpha remained virtually unchanged in comparison with healthy control subjects , WBCCs of colorectal cancer patients secreted significantly lower amounts of IFN alpha ( P < 0 . 005 ) , IFN gamma ( P < 0 . 0001 ) , IL 1alpha ( P < 0 . 0001 ) and IL 2 ( P < 0 . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokines investigated included interleukin ( IL ) 1alpha , IL 1beta , IL 2 , IL 3 , IL 4 , IL 5 , IL 6 , IL 7 , IL 8 , IL 10 , IL 13 , TNF alpha , TNF beta , IFN gamma , TGF beta 2 , G CSF , GM CSF and SCF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha and IFN gamma down regulate the expression of the metastasis associated bi functional 37LRP / p40 gene and protein in transformed keratinocytes . ^^^ Interestingly , despite hallmarks of a housekeeping gene , we show that the 37 LRP / p40 gene promoter can be down regulated by two potentially anticancerous cytokines , TNF alpha and IFN gamma . ^^^ Our data suggest multiple regulation levels in the control of the 67LR / 37LRP / p40 molecule expression and uncover new clues for the understanding of both the control of expression of this metastasis associated molecule and the IFN gamma and TNF alpha anticancerous action . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of several proinflammatory cytokines including TNF alpha , IL 6 , IL 1beta , and IFN gamma were also elevated in Av exposed animals and modulated by dexamethasone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Significantly , T cell lines as well as Th 1 and Th 0 clones synthesized TNF alpha and IFN gamma after this treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
High dose TNF alpha plus chemotherapy , with or without IFN gamma , can be safely administered regionally through isolated limb perfusion . ^^^ Dual targeting is involved ; TNF alpha and IFN gamma induce apoptosis of angiogenic endothelium , while melphalan induces apoptosis of tumour cells . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma did not affect LPS induced TNF alpha and MCP 1 production , but increased baseline MCP 1 production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have shown here that there is a small but consistent induction of some cytokines ( TNF alpha , IFN gamma and IL 2 ) when human blood is directly exposed to O 3 concentrations up to 100 micrograms / ml per g of blood . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Whereas enhanced tumor necrosis factor alpha production was also observed in some patients after IL 12 stimulation , the elevation of tumor necrosis factor alpha was induced only in blood samples that showed IL 12 stimulated IFN gamma production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
STAT 1alpha containing complexes play a predominant role in induction of IFN gamma inducible protein , 10 kDa ( IP 10 ) by IFN gamma alone or in synergy with TNF alpha . ^^^ Human IFN gamma inducible protein , 10 kDa ( hIP 10 ) and murine IP 10 ( mIP 10 ) genes are induced by IFN gamma alone , and synergistically induced by TNF alpha and IFN gamma . ^^^ The requirement of p 48 for hIP 10 induction by IFN gamma , with or without TNF alpha , was demonstrated using p 48 deficient U2A cells . ^^^ Genomic in vivo footprinting revealed IFN gamma / TNF alpha inducible binding at the ISRE consistent with the presence of p 48 and associated factors , but not with IRF 1 . ^^^ Induction of hIP 10 by TNF alpha / IFN gamma also required NFkappaB binding sites , which were protected in vivo and bound p 65 homodimeric NFkappaB in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the effects of dexamethasone or indomethacin on the NADPH oxidase activity , cytochrome b 558 content , and expression of genes encoding the components gp 91 phox and p 47 phox of the NADPH oxidase system in the human monocytic THP 1 cell line , differentiated with IFN gamma and TNF alpha , alone or in combination , for up to 7 days . ^^^ IFN gamma and TNF alpha , alone or in combination , caused a significant up regulation of the NADPH oxidase system as reflected by an enhancement of the PMA stimulated superoxide release , cytochrome b 558 content , and expression of gp 91 phox and p 47 phox genes on both days 2 and 7 of cell culture . ^^^ Noteworthy was the tremendous synergism between IFN gamma and TNF alpha for all studied parameters . ^^^ Indomethacin inhibited only the PMA stimulated superoxide release of THP 1 cells differentiated with IFN gamma and TNF alpha during 7 days . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Astrocytes contribute to the structural integrity of the BBB and can be induced to express VCAM 1 and ICAM 1 in response to cytokines such as TNF alpha , IL 1beta , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The asthma group had higher TNF alpha and IFN gamma production during infection ( P < . 05 ) ; changes in IL 8 and IL 10 levels were not significant , and IL 4 was not detected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum levels of interferon gamma ( IFN gamma ) , interleukin 2 ( IL 2 ) , interleukin 2 receptor ( IL 2R ) , interleukin 10 ( IL 10 ) , tumor necrosis factor alpha ( TNF alpha ) , and interleukin 1beta ( IL 1beta ) were measured by enzyme linked immunosorbent assay ( ELISA ) in 55 untreated leprosy patients and 35 reactional leprosy patients , in addition to 20 age and sex matched healthy controls . ^^^ When the two poles were compared , tuberculoid leprosy ( TT ) patients showed significantly higher levels of IFN gamma and TNF alpha with significant negative correlations with the bacterial index ( BI ) , whereas lepromatous leprosy ( LL ) patients showed significantly higher serum levels of IL 2R , IL 10 , and IL 1beta with significant positive correlations with the BI . ^^^ CONCLUSIONS : In leprosy patients , both IFN gamma and TNF alpha are immunoprotective , whereas IL 2R , IL 10 , and IL 1beta are immunosuppressive . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using the ELISA technique , serum levels of the cytokines interferon gamma ( IFN gamma ) , interleukin 10 ( IL 10 ) and tumour necrosis factor alpha ( TNF alpha ) were investigated . ^^^ The MCTD patients had the highest serum levels of IL 10 , but also had elevated IFN gamma and TNF alpha levels similar to the RA patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Use of neutralizing antibodies and genetically deficient mice showed that IFN gamma ( but not TNF alpha ) mediated rmIL 12 induced immune suppression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our results show that CH inhibits the mixed lymphocyte reaction ( MLR ) between allogeneic PBMCs , production of inflammatory cytokines such as TNF alpha , interleukin 1 beta ( IL 1 beta ) and interferon gamma ( IFN gamma ) in mixed lymphocyte culture and natural killer cell activity , and , further , reduces the number of alloreactive CTL precursors . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) production was normal in response to endotoxin and to PHA but was not augmented by the addition of IFN gamma . ^^^ These patients demonstrate the critical role that the IFN gamma receptor plays in the regulation of IFN gamma , IL 12 , and TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The proinflammatory cytokines , including tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) , are increased in heart failure and sepsis , clinical conditions for which the 4 anesthetic ketamine is useful . ^^^ In this study , we evaluated the effect of ketamine alone , TNF alpha and IFN gamma , and ketamine plus TNF alpha and IFN gamma , on isoproterenol ( ISO , a betaAR agonist ) and forskolin ( FSK , an activator of AC ) induced intracellular accumulation of cAMP . ^^^ Pretreatment with either cytokine alone did not significantly affect ISO or FSK induced intracellular cAMP accumulation , whereas the combination of TNF alpha and IFN gamma caused a significant ( P < 0 . 05 compared with untreated cells ) reduction . ^^^ Pretreatment of the H9c2 cells with ketamine , plus the combination of TNF alpha and IFN gamma , inhibited the reduction of ISO or FSK induced intracellular cAMP accumulation caused by the proinflammatory cytokines alone . ^^^ These results demonstrate that the combination of the proinflammatory cytokines TNF alpha and IFN gamma reduce poststimulation ( ISO or FSK ) intracellular cAMP accumulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There was increased upregulation of IFN gamma , IL 1 , and tumor necrosis factor alpha ( TNF alpha ) mRNAs in the lungs of IL 10 KO mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Unlike the down regulation of TNF alpha under such conditions , the mechanism of IL 12 suppression during endotoxin tolerance is not dependent upon IL 10 or transforming growth factor beta , nor is IL 12 production rescued by IFN gamma or granulocyte macrophage colony stimulating factor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found up regulation of local cytokine genes , including those of Th 1 type ( IL 1beta , TNF alpha , IL 2 , IFN gamma , IL 6 ) , as well as IL 10 and IL 12 ( p 40 ) , in the tissue infiltrating cells during the course of autoimmune sialadenitis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha was chosen as the transcriptionally inducible target gene to be quantified in alveolar macrophages of control rat lung , as well as low and high challenge lungs stimulated by endotoxin and IFN gamma nebulization . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After 24 hs , the plasma was assayed for tumor necrosis factor alpha ( TNF alpha ) , IFN gamma , IL 1beta , IL 1 receptor antagonist ( IL 1Ra ) , and IL 8 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , the DC derived from cultures with IFN gamma , loaded with an immunogenic peptide derived from the HER2 / neu protein [ HER 2 ( 9466 ) ] , only induced low levels of TNF release and weak proliferative responses in a specific cytotoxic CD 8 ( + ) T lymphocyte clone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Other cytokines tested [ interferon gamma ( IFN gamma , tumour necrosis factor alpha ( TNF alpha ) , transforming growth factor beta 1 ( TGF beta 1 ) and IL 6 ] did not affect CD 44 core epitope expression in the cell lines tested . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , pre exposure to tumor necrosis factor ( TNF ) inhibited IFN gamma priming for production of high levels of IL 12 by macrophages in vitro . ^^^ To determine whether TNF inhibition of IFN gamma induced IL 12 production contributed to the resolution of an inflammatory response in vivo , the response of TNF+ / + and TNF / mice injected with Corynebacterium parvum were compared . ^^^ IFN gamma inhibition of chemokine production and inhibition of IFN gamma induced IL 12 production by TNF provide potential mechanisms by which these cytokines can exert anti inflammatory / repair function ( s ) . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunoassays of supernatants from stromal cells revealed that IL 6 was produced constitutively , and its production was increased by treatment with TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The presence of IL 10 completely suppressed the production of IFN gamma and reduced that of TNF alpha , IL 6 , and IL 8 . ^^^ Thus , IFN gamma , TNF alpha , IL 8 , and IL 6 are produced in a single whole blood culture , making correlations in the synthesis of a T helper type 1 cytokine and proinflammatory cytokines with disease activity possible in a single culture . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The CTL line was shown to be phenotypically stable in terms of high levels of cytokine ( IFN gamma , tumor necrosis factor , and granulocyte macrophage colony stimulating factor ) production , expression of homing adhesion molecules , ability to lyse peptide pulsed targets , and ability to lyse human carcinoma cells endogenously expressing CEA in a MHC restricted manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We then examined the association of this model with various cytokines , such as tumor necrosis factor alpha , interleukin ( IL ) 1alpha , IL 1beta , IFN gamma , IL 6 , and leukemia inhibitory factor , and identified human IL 6 , which was produced by the tumor cells , as a mediator of cachexia . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Monocyte mediated cytotoxicity ( determined in a 72 h111In release assay ) and the circulating levels of tumor necrosis factor alpha ( TNF alpha ) , interleukin ( IL ) 1beta , IL 6 , IFN gamma , C reactive protein , and beta 2 microglobulin were determined in 14 melanoma patients treated with multilamellar vesicle liposomes containing muramyl tripeptide phosphatidylethanolamine , 4 mg twice a week for 12 weeks . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lewis rats with EAN have elevated PNS myelin reactive interferon gamma ( IFN gamma ) production , TNF alpha mRNA expression , and increased B cell responses to PNS myelin antigens , but low PNS myelin reactive transforming growth factor beta ( TGF beta ) and interleukin ( IL ) 10 mRNA expression in lymph node mononuclear cells ( MNC ) . ^^^ In contrast , resistance to EAN in SD rats is associated with reduced BPM and P 2 peptide reactive IFN gamma production , TNF alpha mRNA expression , and suppressed B cell responses to PNS myelin antigens as well as up regulation of TGF beta and IL 10 mRNA expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The administration of AOB significantly inhibited the LPS induced hepatic injury and generation of HNE modified proteins and increased the survival rate of endotoxemic rats without affecting NO generation and plasma levels of tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have previously shown that herpes simplex virus type 2 ( HSV 2 ) infection of macrophages synergistically enhances IFN gamma induced NO production , and we now extend these findings by providing evidence that virus induced tumour necrosis factor ( TNF ) alpha mediates activation of the transcription factor nuclear factor ( NF ) kappaB , which in turn is responsible for the synergistic effect . ^^^ HSV 2 infection and IFN gamma stimulation of macrophages synergistically induced TNF alpha secretion and nuclear translocation of NF kappaB , which bound to a sequence corresponding to a kappaB site in the iNOS promoter . ^^^ Thus , our data indicate that NF kappaB activation , through virus induced autocrine TNF alpha secretion , is responsible for the synergistic effect of HSV 2 infection on IFN gamma induced NO production , and that such activation might constitute a mechanism by which high output NO production is targeted to infectious foci . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we assessed the production of several cytokines ( IL 4 , IFN gamma , and TNF alpha ) in vivo and in vitro using genetically susceptible ( BALB / c ) or resistant ( C57BL / 6 ) mice infected with cloned Trypanosoma congolense and the role of these cytokines in pathogenesis of this infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In mice treated with these mAbs , the production of inflammatory and Th 1 derived cytokines such as TNF alpha and IFN gamma in the spleen cells was decreased , and that of Th 2 derived cytokines such as IL 4 and IL 10 was increased . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Injection of PEA to mice caused hepatocyte apoptosis , an increase in plasma transaminase activities , and the release of TNF , IFN gamma , IL 2 , and IL 6 into the circulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To determine whether pro inflammatory cytokines modulate intercellular adhesion molecule 1 ( ICAM 1 ; CD 54 ) expression on cultured primary human corneal epithelial cells ( HCEs ) , confluent HCEs were treated with various concentrations of interferon gamma ( IFN gamma ) , interleukin 1alpha ( IL 1alpha ) , IL 1beta , IL 4 , tumor necrosis factor alpha ( TNF alpha ) , or combinations over time . ^^^ In summary , ICAM 1 was modulated on primary human corneal epithelial cells by the cytokines IFN gamma and TNF alpha in a dose and time dependent fashion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The results of this in vitro study suggest that beta chemokine mRNA expression by adult rat astrocytes can be induced by LPS or proinflammatory cytokines , while regulatory cytokines , such as TGF beta 1 and IL 10 , down regulate astrocyte derived beta family chemokine mRNA expression induced by LPS , IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) are necessary in the early stages of induction of CD 4 and CD 8 cytotoxic T cells by Mycobacterium leprae heat shock protein ( hsp ) 65 kD . ^^^ Thus , IL 6 , IL 2 , IFN gamma or TNF alpha did not modify the generation of hsp 65 CTL from either MB ( with or without an erythema nodosum episode ( ENL ) ) or PB . ^^^ In MB , IL 6 plus IFN gamma or IL 2 increased both CD 4 and CD 8 CTL , while TNF alpha plus IFN gamma up regulated only CD 4 CTL . ^^^ Down regulation of CTL was observed by addition of IL 4 , IL 10 , anti IFN gamma or anti TNF alpha in N controls . ^^^ Our data demonstrate that IFN gamma and TNF alpha must be present for at least the first 60 h of the induction stage in order to generate full hsp 65 CTL . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PHIG greatly reduced the production of IL 12 , interferon gamma ( IFN gamma ) and other cytokines from SPE A stimulated PBMC , while exogenous IL 12 , but neither IL 1 nor tumour necrosis factor alpha ( TNF alpha ) , restored IFN gamma production inhibited by PHIG . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
About 30 % of unstimulated PBLs expressed Fas , and the expression was augmented by interleukin 1beta ( IL 1beta ) , IL 2 , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , or PMA plus ionomycin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , the effect of 1 , 25 ( OH ) 2D3 on B 7 molecule expression in combination with lipopolysaccharide ( LPS ) or cytokines , including interleukin 10 ( IL 10 ) , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) , was studied . ^^^ The 1 , 25 ( OH ) 2D3 induced B7 . 2 down regulation was still detectable when monocytes were activated by IL 10 , IFN gamma and TNF alpha but not with LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The kinetics of selected cytokines [ interleukin 2 ( IL 2 ) , IL 6 , interferon gamma ( IFN gamma ) and tumour necrosis factor ( TNF ) ] and phenotype and cell cycle analysis of T lymphocytes were determined in Long Evans rats administered a single intraperitoneal ( i . p . ) dose of either 50 microg or 500 microg of SEB . ^^^ These studies demonstrate that the cytokine responses in Long Evans rats exposed to a superantigen are somewhat similar to those that occur in mice and humans , e . g . a rapid short increase in the production of IFN gamma and TNF that was accompanied by an increase in the production of IL 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stress is associated with increased expression of interleukin 1 beta ( IL 1 beta ) and tumor necrosis factor alpha ( TNF alpha ) , and reduced expression of IL 2 , interferon gamma ( IFN gamma ) , major histocompatability complex ( MHC ) class 2 molecules and natural killer cell activity ( NKA ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Release of NO after stimulation with lipopolysaccharide ( LPS ) , interleukin lbeta ( IL 1beta ) , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) was determined by Griess reagents . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the effect of TNF alpha , IL 1alpha and IFN gamma on two neuroblastoma ( NB ) cell lines ( SK N SH and SK N MC ) . ^^^ These lines responded differentially to IL 1alpha , TNF alpha and IFN gamma for MCP 1 and IL 8 production and expression of the ICAM 1 and VCAM 1 adhesion molecules . ^^^ The expression of ICAM 1 on SK N MC was up regulated by TNF alpha or IFN gamma , while IL 1alpha also upregulated ICAM 1 on SK N SH cells . ^^^ VCAM 1 expression on SK N SH was induced by IL 1alpha and TNF alpha and IFN gamma synergized with TNF alpha in this respect on both NB cell lines . ^^^ These results suggest that mechanisms for chemokine production and VCAM 1 and ICAM 1 upregulation by inflammatory cytokines differ and IFN gamma , in conjunction with TNF alpha , stimulate neural cell responses ( high MCP 1 and VCAM 1 and decreased IL 8 ) favouring mononuclear cell recruitment . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Earlier experiments in Trypanosoma cruzi infected rats showed that recombinant rat ( Rr ) interferon ( IFN ) gamma given shortly after infection ameliorated acute disease without modifying the serum titers of endogenously synthesized IFN gamma and tumor necrosis factor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study we have analyzed the local response of different cytokines i . e . , the proinflammatory interleukin 1beta , ( IL 1beta ) , IL 6 , tumor necrosis factor alpha , and IL 8 ; the immunoregulatory gamma interferon ( IFN gamma ) ; and IL 4 ; and the anti inflammatory transforming growth factor beta ( TGF beta ) in antral biopsy specimens from H . pylori infected duodenal ulcer ( DU ) patients and asymptomatic ( AS ) carriers ( i . e . , with chronic gastritis only ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PROCEDURE : Cells from the anterior pituitary gland were cultured in the presence of recombinant ovine or bovine cytokines ( interleukin [ IL ] 1alpha , IL 1beta , and IL 2 ) , tumor necrosis factor alpha ( TNF ) , and interferon gamma ( IFN gamma ) . ^^^ Interleukin 2 , TNF , and IFN gamma did not have a detectable effect on release of LH . ^^^ Interleukin 2 , TNF , and IFN gamma do not have a direct effect on release of LH ; therefore , they do not mediate this effect of endotoxin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Differential nitric oxide and TNF alpha production of murine Kupffer cell subfractions upon priming with IFN gamma and TNF alpha . ^^^ METHODS : In the present study , we demonstrate a shift in response of KC fractions separated on cell size by countercurrent elutriation upon priming with tumor necrosis factor alpha ( TNF alpha ) or interferon gamma ( IFN gamma ) . ^^^ A strong synergy , with respect to production of NO , was observed when KC subfractions were exposed to a combination of TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After stimulation with IFN gamma or TNF alpha , human myoblasts selectively express BB 1 , but not B7 . 1 or B7 . 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Among macrophage activators tested ( M CSF , granulocyte macrophage CSF , IL 3 , TNF alpha , IFN gamma , and LPS ) , only M CSF could up regulate the p 26 HRF synthesis by cultured macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These cells , which proliferated , exhibited a profound increase in IL 4 production that was accompanied by a significant decrease in IFN gamma and TNF alpha production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human NK cells have been shown to produce cytokines ( e . g . , IFN gamma and TNF alpha ) and the chemokine macrophage inflammatory protein ( MIP ) 1alpha following stimulation with the combination of two monokines , IL 15 plus IL 12 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Thus , in the present study we have measured and established the values of IL 1alpha , IL 2 , IL 3 , IL 4 , IL 6 , IL 10 , IL 12 , GM CSF , TNF alpha and IFN gamma during all phases of human pregnancy ( first , second and third trimester of pregnancy , labour , abortions of the first trimester ) as well as in the non pregnant control state . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Relative to unstimulated control tissues , there was a significant increase in TNF alpha , IFN gamma , IL 12 , and IL 10 mRNAs in spleen with down regulation of IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interestingly , cells obtained from draining lymph nodes in AG 490 treated animals and stimulated with antigen secreted two times more IFN gamma and four times more IL 10 , when compared with control animals , whereas no difference was observed in TNF alpha production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Various Kb binding TNP and DNP peptides acted as partial agonists , cross reactively stimulating individual clones for cytotoxicity and IFN gamma secretion , but failing to induce proliferation or TNF alpha production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The combination of cristobalite plus TNF alpha led to an additive increase in MCP 1 and MIP 2 , whereas cristobalite plus IFN gamma or LPS had a synergistic effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Protein levels of the cytokines interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) , transforming growth factor beta ( TGF beta ) , IL 4 and IL 10 were detected at day 3 post inoculation ( p . i . ) with maximum induction at day 8 . ^^^ Aabs dose dependently inhibited IFN gamma induced MHC expression by peritoneal macrophages and TNF alpha mediated L 929 cytotoxicity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
High levels of tumour necrosis factor alpha ( TNF alpha ) and IFN gamma were present in the sera of HIV 1 infected patients with active VL . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Since cytokines are of crucial significance in this process , we decided to examine the role of stem cell factor ( SCF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) , granulocyte colony stimulating factor ( G CSF ) , and interleukin 1 alpha ( IL 1 alpha ) , known for their stimulatory effects on in vitro hematopoiesis ; and transforming growth factor beta ( TGF beta ) , interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) , known for their inhibitory effect , in the anemia of GCA . ^^^ RESULTS : Consistent constitutive gene expression was detected in the marrow stromal cells of all control samples for SCF , GM CSF , IL 1 alpha , TGF beta and TNF alpha , while only 2 / 6 expressed IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increasing concentrations of bovine TSH ( 0 . 1 , 1 , 10 , 50 and 100 mU / l ) , with or without tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) or interleukin 1 alpha ( IL 1 alpha ) were added to FRTL 5 cells previously deprived of TSH for a minimum of 5 days . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro experiments proved that although IFN gamma did not increase the production of Mn SOD by itself , concomitant treatment with both IFN gamma and TNF alpha enhanced the Mn SOD production in QR 32 cells greatly . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PPD positive and anergic subjects in the placebo arms of the preventive therapy study underwent repeat skin testing and immunologic testing including measurement of MTB culture filtrate ( CF ) stimulated interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) levels in whole blood culture supernatants . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Hepatitis B virus ( HBV ) gene expression is downregulated in the liver of HBV transgenic mice by a posttranscriptional mechanism that is triggered by the local production of gamma interferon ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) during intrahepatic inflammation ( hepatitis ) . ^^^ In this study , we identified three HBV RNA binding liver nuclear proteins ( p 45 , p 39 , and p 26 ) the relative abundance of which correlates with the abundance of HBV RNA in response to the induction of IFN gamma and TNF alpha . ^^^ The presence of p 45 correlates directly with the presence of HBV RNA , being detectable under baseline conditions when the viral RNA is abundant and undetectable when the viral RNA disappears in response to IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma itself also induced NO , TNF alpha and IL 6 production from RAW 264 . 7 cells . ^^^ But TNF alpha responses of RAW cells against these preparations were almost equal when cultured alone or in combination with IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A feature of the results was that while DMXAA treatment induced both IFN gamma and tumour necrosis factor ( TNF ) in serum , the increase was > 20 fold higher in IFN gamma R0 / 0 mice than in wild type mice . ^^^ In contrast , mRNA levels for IFN gamma and TNF were similar in the two mouse strains , suggesting that the concentrations of these cytokines were controlled by a post transcriptional mechanism . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study was undertaken to assess whether gamma linolenic acid ( GLA ) in the form of evening primrose oil ( EPO ) could affect rat serum cytokines , interferon gamma ( IFN gamma ) , monocyte chemotactic protein 1 ( MCP 1 ) and tumour necrosis factor alpha ( TNF alpha ) . ^^^ In the presence of GLA , the IFN gamma and MCP 1 levels were significantly decreased in contrast to the control group of TNF alpha , which was significantly stimulated . ^^^ The observations indicate that GLA may modulate the level of serum IFN gamma , MCP 1 and TNF alpha , which may be a worthwhile line of treatment in certain human diseases . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These clinical effects were associated with the down modulation of CNS antigen induced T cell responses and production of proinflammatory cytokines ( IFN gamma and TNF alpha ) as well as with upregulation of IL 4 ( except in spleen MNC ) , IL 10 and TGF beta in both spleen MNC and the spinal cord . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Splenic Mphi isolated from mice 7 days after thermal injury produced higher levels of RNI than Mphi from sham mice when stimulated with lipopolysaccharide ( LPS ) or interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) in combination . ^^^ RNI production in response to IFN gamma or IFN gamma and TNF alpha in combination was enhanced by PGE 2 in both populations , however , the effect was markedly greater in Mphi from injured mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum levels of reactive nitrogen intermediates ( RNI ; nitrate + nitrite ) , interferon gamma ( IFN gamma ) and tumour necrosis factor ( TNF ) were measured in 177 Papua New Guinean children with different clinical manifestations of malaria . ^^^ Similarly , the levels of IFN gamma were highest among the cerebral and lowest among the asymptomatic patients ( mean log TNF levels 0 . 338 pg / mL vs 0 . 054 pg / mL ; P < 0 . 0001 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 13 may substitute for IL 4 , whereas IL 10 , TNF alpha or IFN gamma inhibited the generation of MoDCs . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Culturing these cells with IFN gamma ( 100 U / ml ) and TNF alpha ( 1000 U / ml ) , alone or in combination for 7 days , caused a modest increase in their NADPH oxidase activity ( P > 0 . 05 in all situations ) . ^^^ Gene expression studies also show a modest increase in expression and transcription rates of gp 91 phox and p 47 phox genes in normal EBV transformed B cells cultured with IFN gamma ( 100 U / ml ) and TNF alpha ( 1000 U / ml ) , alone or in combination for 7 days . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) induced CD11b / CD18 expression on leukocytes and interferon gamma ( IFN gamma ) induced ICAM 1 expression on tumor cells may play an important role in leukocyte mediated tumor destruction . ^^^ It has also been shown that OK 432 induces various cytokines , such as TNF alpha , TNF beta , IFN alpha IFN gamma , interleukin 1 ( IL 1 ) , IL 2 , IL 6 , IL 12 , tumor growth inhibitory factor ( s ) ( TGIF ) , and possibly unknown apoptosis inducing factor ( s ) . ^^^ Tumor necrosis factor alpha ( TNF alpha ) induced CD11b / CD18 expression on leukocytes and interferon gamma ( IFN gamma ) induced ICAM 1 expression on tumor cells may play an important role in leukocyte mediated tumor destruction . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
One course of therapy was composed of the intravenous administration of either IFN gamma or TNF SAM 2 followed 3 hours later by OK 432 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Production of TNF alpha and IFN gamma in the culture was significantly lower in 9 patients ( 1188 + / 634 pg / ml and 46 + / 84 pg / ml ) than in 10 healthy subjects ( 2491 + / 1037 pg / ml and 295 + / 219 pg / ml ) ( p < 0 . 002 and p < 0 . 003 ) , while the production of IL 4 was slightly higher in cancer patients than in the healthy subjects . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : TNF alpha , IL 1beta , CINC , and IFN gamma were detected in experimental vitreous samples at increased levels that peaked at 24 hours . ^^^ TNF alpha , IL 1beta , and CINC declined at 48 hours , but IFN gamma remained elevated . ^^^ CONCLUSIONS : S . aureus induced the expression of TNF alpha , IL 1beta , CINC , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
THC decreased constitutive production of IL 8 , MIP 1alpha , MIP 1beta , and RANTES and phorbol ester stimulated production of TNF alpha , GM CSF and IFN gamma by NK cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , expression of CD1a , CD 4 , CD 8 , CD 28 , CD 54 , CD 80 , CD 86 and HLA DR on migrating lymph cells and cytokine levels ( IL 1alpha , IL 1beta , IL 2 , IL 6 , IL 8 , IL 10 , IL 13 , TNF alpha and IFN gamma ) in the afferent lymph were analyzed by cytofluorometry and ELISA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF ) and interferon gamma ( IFN gamma ) have been shown to be crucial mediators in the pathogenesis of this neurovascular complication . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha and IFN gamma render microglia sensitive to Fas ligand induced apoptosis by induction of Fas expression and down regulation of Bcl 2 and Bcl xL . ^^^ Whereas resting microglia are resistant to Fas ligand ( FasL ) treatment , induction of FasL mediated apoptosis was achieved by treatment with TNF alpha or IFN gamma . ^^^ Activation of microglia by TNF alpha and IFN gamma was also accompanied by increased amounts of mRNA for the apoptosis inhibitor FLIP , an effect which did not protect the cells from FasL induced apoptosis . ^^^ By their expression of FasL and their TNF alpha / IFN gamma dependent sensitivity to the pro apoptotic effect of exogenous FasL , microglial cells may influence the course of T cell mediated diseases of the CNS . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Transcriptional suppression of matrix metalloproteinase 2 gene expression in human astroglioma cells by TNF alpha and IFN gamma . ^^^ The proinflammatory cytokines TNF alpha and IFN gamma individually can inhibit constitutive MMP 2 expression , and function in an additive manner for near complete inhibition of MMP 2 expression . ^^^ Inhibition of MMP 2 mRNA levels by TNF alpha and IFN gamma is not due to destabilization of the MMP 2 message ; rather , inhibition is mediated at the transcriptional level . ^^^ Furthermore , TNF alpha / IFN gamma inhibition of MMP 2 expression results in decreased invasiveness of the human astroglioma cells through an extracellular matrix . ^^^ These results raise the possibility that TNF alpha and IFN gamma may have beneficial effects in attenuating astroglioma invasive properties . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha , IFN gamma , or IL 1beta treatment of cocultures also induced significant migration of monocytes that correlates with the induction of MCP 1 protein . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Preincubation of cells with antibodies to tumor necrosis factor alpha or the interleukin 1 receptor partially reduced the priming effect of IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro stimulation of lymphocytes isolated from soluble beta glucan treated mice with lipopolysaccharide ( LPS ) resulted in enhanced production of interleukin 6 ( IL 6 ) and suppressed production of tumor necrosis factor alpha ( TNF alpha ) , while stimulation of these cells with staphylococcal enterotoxin B ( SEB ) or toxic shock syndrome toxin 1 ( TSST 1 ) resulted in enhanced production of gamma interferon ( IFN gamma ) and suppressed production of IL 2 and TNF alpha compared to that in cells isolated from untreated mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increased levels of interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) and interleukin 2 ( IL 2 ) were found in the supernatants of cultures treated with OK 432 in 29 ( 90 . 6 % ) , 20 ( 62 . 5 % ) , and 8 ( 25 . 0 % ) out of 32 patients , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Most subjects had severe gastric injury after aspirin therapy , resulting in a substantially increased production of IL 1beta , IL 6 , and IL 8 but not of TNF alpha and IFN gamma in the antral mucosa . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A cell specific microdissection technique in combination with reverse transcription polymerase chain reaction ( RT PCR ) and Southern hybridization using 32P labeled cytokine gene specific probes was utilized to measure cytokine messenger RNA expression in individual cell populations of patients and healthy controls . mRNAs for interleukin 2 ( IL 2 ) , interleukin 6 ( IL 6 ) , interleukin 10 ( IL 10 ) , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) and transforming growth factor beta 1 ( TGF beta 1 ) were detected in the epithelial cells ( acini and ducts ) and lymphoid cells of the labial minor salivary glands of pSS patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Long term exposure of muscle cells to tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) and lipopolysaccharide ( LPS ) greatly increased iNOS mRNA expression and NO production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CONCLUSIONS : Cytokines may contribute to pregnancy immunotolerance ( IFN alpha , IFN beta , and IL 10 ) , viral resistance ( IFNs ) , hormone secretion ( IL 1 and IL 6 ) , and cellular remodeling ( IFN gamma and TNF alpha ) within the chorionic villous . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor surveillance : expression of the transporter associated with antigen processing ( TAP 1 ) in ex vivo human tumor samples and its elevation by in vitro treatment with IFN gamma and TNF alpha . ^^^ Tumors exposed in vitro for a short time to IFN gamma and TNF alpha had elevated TAP 1 levels in 12 out of 30 experiments . ^^^ Short term in vitro treatment with IFN gamma and TNF alpha elevated MHC class 1 expression in several tumors . ^^^ In vitro treatment with IFN gamma and TNF alpha upregulated thus the expression of MHC class 1 and TAP 1 in a fraction of the tumor samples . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MPhi incubated in the presence of CPE induced the synthesis of interleukin 1 ( IL 1 ) , interleukin 6 ( IL 6 ) , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) , but not interleukin 2 ( IL 2 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The BAL concentrations of the nine cytokines evaluated for the more and less affected lungs were compared : interleukin 6 ( IL 6 ) , IL 8 , IL 12 , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) showed significant differences ( p ranged between 0 . 016 and 0 . 0007 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Propagermanium treatment induced significant inhibition of subsequent TNF alpha production about 10 h after Con A injection , without affecting IFN gamma , interleukin ( IL ) 10 , IL 4 and IL 12 production . ^^^ Therefore , Mphi were cultured with liver sinusoidal endothelial cells ( SEC ) and the effect of propagermanium on TNF alpha production in the presence of IFN gamma was determined . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The purpose of this study was to evaluate immune function through the assessment of lymphocyte subpopulations ( total T cells , major histocompatibility complex [ MHC ] 1 and 2 restricted T cells , B cells , NK cells , MHC 2 restricted T cell derived naive and memory cells , and several MHC 1 restricted T cell activation markers ) and the measurement of cytokine gene expression ( interleukin 2 [ IL 2 ] , IL 4 , IL 6 , IL 10 , IL 12 , gamma interferon [ IFN gamma ] , and tumor necrosis factor alpha [ TNF alpha ] ) from peripheral blood lymphocytes . ^^^ In addition , veterans with CFS had significantly higher levels of IL 2 , IL 10 , IFN gamma , and TNF alpha than the controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Assays of neopterin , beta 2 microglobulin , soluble interleukin 2 receptor , and soluble tumor necrosis factor receptor type 2 as well as of the cytokines tumor necrosis factor alpha and gamma interferon ( IFN gamma ) were evaluated by using serum and plasma samples of human immunodeficiency virus ( HIV ) positive and HIV negative subjects . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1 alpha , TNF alpha and IFN gamma at concentrations of 10 ( 5 ) U / l all inhibited TSH induced NIS gene expression and iodide uptake . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The Th 2 hypothesis of allergy ( 4 ) considers atopy as a Th 2 driven hypersensitivity reaction to allergens of complex genetic and environmental origins , in which the Th 1 lineage , normally driven by IL 2 , TNF , and IFN gamma is deficient , and in which a predominant Th 2 response is seen that is driven by IL 4 , IL 13 , IL 5 , and IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lymphocytes were examined for three reasons : ( 1 ) for their ability to inhibit the growth of Candida albicans ; ( 2 ) for their ability to proliferate in response to a mitogen ; and , ( 3 ) their cytokine profile ( vis . , production of IL 2 , IFN gamma and TNF alpha ) . ^^^ The patients were diagnosed as HIV+ within the past 0 . 5 9 years . d FEN was found to augment the capacity of CD8+ lymphocytes to inhibit the growth of the opportunistic microbial pathogen , C . albicans . d FEN enhanced the capacity of CD4+ lymphocytes to proliferate in response to the mitogen , Concanavalin A , and to increase the amount of IL 2 produced by CD4+ and CD8+ lymphocytes from AIDS patients . d FEN increased the number of CD4+ and CD8+ lymphocytes that produced IFN gamma from either non AIDS or AIDS patients and increased the number of AIDS patient ' s CD8+ lymphocytes that produce TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
On the other hand , MDA appears to be a combination of interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) , since neutralizing antibodies against these two cytokines completely abolish MDA induced CD 41 expression . ^^^ In addition , either recombinant human IFN gamma or TNF alpha alone is capable of inducing CD 41 and CD 42 expression on HIMeg 1 cells . ^^^ In combination , IFN gamma and TNF alpha induce a maximal level of CD 41 and CD 42 expression which is also accompanied by an increase in cell size and DNA ploidy level . ^^^ Thus , our studies indicate that IFN gamma / TNF alpha is capable of inducing megakaryocytic differentiation of the HIMeg 1 cell line and that HIMeg 1 is a good system for studying the molecular mechanism mediating megakaryocytic differentiation . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Preoperatively and on the 1st , 3rd , and 7th postoperative days , the cell percentages of lymphocyte subpopulations , the IL 2 receptor expression , and the IL 1 , IL 2 , TNF alpha and IFN gamma were measured in blood by flow cytometry and ELISA . ^^^ CLP+BT rats showed on Day 3 a decrease of the CD4+ % , an increase of the IL 2R expression directly correlated to the increase of the CD8+ % phenotype , a steady increase of IL 1 levels , a decrease of the TNF alpha levels on the 1st and 3rd days , and a decrease of the IL 2 and IFN gamma on Day 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To determine if activation of macrophage function would result in elimination of intracellular H . somnus , BBM were treated with E . coli lipopolysaccharide ( LPS ) or recombinant bovine ( rBo ) cytokines , interferon gamma ( IFN gamma ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , tumour necrosis factor alpha ( TNF alpha ) or interleukin 1beta ( IL 1beta ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The culture supernatants were harvested to determine the synthesis of IgE , interleukin 5 ( IL 5 ) , IL 6 , IL 10 , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Normal bovine peripheral blood mononuclear leukocytes ( PBML ) and fetal bovine muscular cells ( FBMC ) were treated with varying doses of human interferon ( IFN ) alpha , IFN gamma , tumor necrosis factor ( TNF ) alpha and TNF beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Compared with healthy controls , spontaneous and PHA induced IFN gamma and TNF alpha production was significantly decreased in BD whereas spontaneous release of IL 2 , sCD 4 and sCD 8 was significantly increased . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The aim of this study was to verify whether Pasteurella multocida porin can affect the expression and release of IL 1alpha , IL 6 , TNF alpha , IL 4 , IFN gamma , IL 10 and IL 12 by murine splenocytes in vitro . ^^^ P . multocida porin and lipopolysaccharide ( LPS ) were able to induce the release of IL 1alpha , IL 6 , TNF alpha , IFN gamma and IL 12 in a dose dependent fashion . ^^^ The time courses of release showed that P . multocida LPS was able to stimulate the production of IL 1alpha , IL 6 , TNF alpha , IFN gamma and IL 12 earlier than porin and at a greater rate . ^^^ P . multocida porin and LPS were also able to up regulate the mRNA expression of IL 1alpha , IL 6 , TNF alpha , IFN gamma and IL 12 p 40 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Osteoblasts respond to stimulation with interleukin 1 ( IL 1 ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) by production of nitric oxide and prostaglandins ( PGs ) . ^^^ IL 1 , TNF alpha , IFN gamma , and exogenous sodium nitroprusside , a nitric oxide donor , all stimulated PGE 2 production in a dose dependent manner . ^^^ PGE 2 production was blocked by L nitro arginine methyl ester , an inhibitor of nitric oxide production , after IFN gamma stimulation and was partially blocked after TNF alpha stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RT PCR analysis of colonic RNA revealed that treatment with rhIL 11 down regulated expression of proinflammatory cytokines including TNF alpha , IL 1beta , and IFN gamma . rhIL 11 also reduced the level of myeloperoxidase activity in the cecum indicating reduced inflammation . ^^^ After stimulation in vitro with anti CD 3 antibody , spleen cell cultures derived from rhIL 11 treated rats produced less IFN gamma , TNF alpha , and IL 2 than cultures derived from vehicle treated rats . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokines known to regulate and / or be expressed in the anterior pituitary include the inflammatory cytokine family ( IL 1 and its endogenous antagonist , IL 1ra ; TNF alpha , and IL 6 ) , the Th 1 cytokines ( IL 2 and IFN gamma ) , and other cytokines such as LIF , MIF , and TGF beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CD 3 zeta expression is more prominent in advanced stage of the disease and correlates with reduced proliferative response , IFN gamma , IL 2 , TNF alpha production . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , the cells obtained from SCLC group produced little detectable levels of TNF alpha ( median 12 . 0 , range 3 45 U / ml ) , IFN gamma ( median 3 , range 3 12 U / ml ) and IL 6 ( median 15 , range 6 45 U / ml ) in response to LPS and interferons , mainly IFN alpha ; ( median 3 , range 3 12 U / ml ) in response to NDV . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated serum level of interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) and interleukin 6 ( IL 6 ) using an enzyme linked immunosorbent assay ( ELISA ) in 59 patients with systemic lupus erythematosus ( SLE ) and 16 healthy controls . ^^^ We examined a possible association between serum levels of these cytokines and SLE activity , as well as correlation between IFN gamma concentration and the level of TNF alpha and IL 6 and also IL 6 and TNF alpha . ^^^ We found positive correlation between serum concentration of TNF alpha and IL 6 with SLE activity and no such correlation with IFN gamma . ^^^ We also observed positive correlation between serum levels of IFN gamma and TNF alpha , IFN gamma and IL 6 as well as TNF alpha and IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Adoptive transfer of autologous tissue derived T cell lines and T cell clones into synovium SCID mouse chimeras augmented the production of IFN gamma as well as TNF alpha in the synovial infiltrates . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Evaluation of the role of mitogen activated protein kinases in the expression of inducible nitric oxide synthase by IFN gamma and TNF alpha in mouse macrophages . ^^^ The expression of inducible nitric oxide synthase ( iNOS ) by macrophages is stimulated by coexposure to IFN gamma and a number of stimuli , including TNF alpha . ^^^ The objective of this study was to systematically evaluate the role of : 1 ) p42mapk / erk2 , 2 ) p 46 c Jun NH 2 terminal kinase / stress activated protein kinase ( p 46 JNK / SAPK ) , and 3 ) p38mapk in the induction of iNOS expression during costimulation of mouse macrophages with IFN gamma and TNF alpha . ^^^ All three kinases were activated during costimulation with IFN gamma and TNF alpha . ^^^ In addition , specific antagonism of the JNK / SAPK upstream kinases MEKK ( mitogen activated protein kinase / extracellular signal regulated kinase kinase kinase ) and MKK 4 ( mitogen activated protein kinase kinase 4 ) with dominant inhibitory mutants blocked transcriptional activation of the iNOS promoter in response to costimulation with IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This was evidenced by a reduction in tissue myeloperoxidase ; reduced transcripts for TNF alpha , IL 1 , IL 12 , and IFN gamma ; and a reduction in the T cell infiltrate . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro the cells from the mice injected and challenged with the venom only released IL 10 while peritoneal macrophages released IL 10 , INF gamma and less amounts of IL 6 ; ( c ) establishment of local inflammation and necrosis induced by the venom , coincides with the peaks of TNF alpha , IFN gamma and NO and the damage was neutralized when the venom was incubated with a monoclonal antibody against a 60 kDa haemorrhagic factor . ^^^ These results suggest that susceptibility to Bothrops atrox venom is genetically dependent but MHC independent ; that IL 6 , IL 10 , TNF alpha , IFN gamma and NO can be involved in the mediation of tissue damage ; and that the major venom component inducers of the lesions are haemorrhagins . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Diverse kinds of stresses ( ultraviolet , irradiation , heat shock and hypoxia ) and biological factors ( TNF alpha , IFN gamma and Fas antibody ) require ceramide generation to execute apoptosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Significantly faster parasite clearance times were associated with in vitro production of IL 10 by acute phase peripheral blood mononuclear cells ( PBMC ) in response to both liver and asexual stage parasite antigens , but not with proliferative , IFN gamma , or TNF responses to the same antigens . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 17 is produced by nickel specific T lymphocytes and regulates ICAM 1 expression and chemokine production in human keratinocytes : synergistic or antagonist effects with IFN gamma and TNF alpha . ^^^ In this study , we investigated whether hapten specific T cells isolated from patients with allergic contact dermatitis ( ACD ) to nickel produce IL 17 and the effects of IL 17 alone or in combination with IFN gamma or TNF alpha on the immune activation of keratinocytes . ^^^ Skin affected with ACD to nickel and skin derived , nickel specific CD4+ T cell lines expressed IFN gamma , TNF alpha , and IL 17 mRNAs . ^^^ Four of seven nickel specific CD4+ T cell clones positive for the skin homing receptor , cutaneous lymphocyte associated Ag , were shown to corelease IL 17 , IFN gamma , and TNF alpha . ^^^ In addition , IL 17 , both directly and in synergism with IFN gamma and / or TNF alpha , stimulated synthesis and release of IL 8 by keratinocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PATIENTS AND METHODS : We have studied the minor salivary glands in 30 patients with pSS , 30 patients with sSS , 19 patients with AD without SS and 18 controls , using immunohistochemical techniques to analyze the molecular expression of CD 3 , CD 4 , CD 8 , CD 20 , CD 25 , CD 14 , CD 56 , CD11a , CDw 50 ( ICAM 3 ) , HLA DR , IL 1 , TNF alpha and IFN gamma in lymphocytic infiltration and epithelial cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 ) expression is upregulated by cytokines such as tumor necrosis factor alpha ( TNF alpha ) , interleukin 1beta ( IL 1beta ) , and interferon gamma ( IFN gamma ) in numerous cell types including the astrocyte , which functions as an immunoregulatory cell within the central nervous system . ^^^ TNF alpha and IL 1beta enhanced ICAM 1 expression at both the mRNA and protein levels , while IFN gamma had a modest enhancing effect . ^^^ However , a synergistic response was noted when IFN gamma was added with either TNF alpha or IL 1beta . ^^^ Using human ICAM 1 deletion constructs and linker scanning mutants , we determined that the NF kappaB element ( 186 bp region ) is critical for both TNF alpha and IL 1beta mediated ICAM 1 expression , while the IFN gamma activation sequence ( GAS ) element at 75 bp region is important for IFN gamma stimulation . ^^^ The synergistic effect between TNF alpha and IFN gamma is dependent on both NF kappaB and GAS elements . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma was the only cytokine that effectively upregulated HLA DR expression in ChWK , which was synergistically enhanced by tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The developing picture is that of an early pro inflammatory type 1 like response ( characterized by the production of IFN gamma and TNF alpha and NO ) which in the short term is able to control the deleterious effects on the liver associated with the arrival of the eggs , followed rapidly by a potent egg induced Th 2 response which simultaneously subsumes the role of protecting the liver and downregulates the production of the inflammatory mediators , thereby averting the serious consequence associated with the continued high level production of NO . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : By means of flow cytometry analysis , we evaluated ICAM 1 and CD 29 expression by WK and HEL epithelial cells in basal condition ( at baseline ) or after IFN gamma or TNF alpha stimulation in the presence or in the absence of terfenadine and fexofenadine . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Finally , Fas was induced on NOD islet cells by incubation with IL 1beta , IFN gamma , and the combination of IL 1beta , TNF alpha , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 6 , IFN gamma and TNF alpha production by liver associated T cells and acute liver injury in rats administered concanavalin A . ^^^ The relationship between the development of acute hepatitis and the production of TNF alpha IFN gamma and IL 6 by liver associated T lymphocytes following intravenous injection of concanavalin A ( Con A ) was studied in rats . ^^^ Following a single injection of Con A , there was a dose and time dependent correlation in the serum levels of serum alanine aminotransferase ( ALT ) , IL 6 , IFN gamma and TNF alpha . ^^^ Increased levels of IL 6 , IFN gamma and TNF alpha were constitutively produced by liver associated CD4+ T cells when cultured . ^^^ These results support the hypothesis that Con A induced liver damage is mediated by CD4+ T cells acting within the liver , at least in part through the secretion of TNF alpha , IFN gamma and IL 6 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Levels of the pro inflammatory cytokines TNF alpha and IFN gamma were measured from the time of infection to the time of complete clearance of non typeable Haemophilus influenzae ( NTHi ) from the lung in immune and non immune rats . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study , the functions of interferon gamma ( IFN gamma ) and tumour necrosis factor ( TNF ) for chemokine regulation during acute lung inflammation were examined . ^^^ Following administration of the superantigen , staphylococcal enterotoxin B ( SEB ) , lung mRNA levels of the chemokines cytokine induced neutrophil chemo attractant ( KC ) , lipopolysaccharide induced CXC chemokine ( LIX ) , macrophage chemotactic protein 1 ( MCP 1 ) , macrophage inflammatory protein ( MIP ) 1alpha and MIP 2 were increased to a similar extent both in controls and in mice deficient for the IFN gamma or 55 000 MW TNF receptors . ^^^ In contrast , interferon inducible protein 10 ( IP 10 ) and monokine induced by IFN gamma ( Mig ) mRNA expression was markedly reduced in mice deficient for IFN gamma or IFN gamma receptor , but not in 55 000 MW TNF receptor knockout mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This protection is known to be mediated by T cell secreted type 1 cytokines IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Consistent with the enhancement of DTH responses , IL 10 KO mice showed stronger and more persistent CD 4 T cell dependent IFN gamma production and significant elevation of IL 12 and TNF alpha production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Alleviation of lipopolysaccharide induced acute liver injury in Propionibacterium acnes primed IFN gamma deficient mice by a concomitant reduction of TNF alpha , IL 12 , and IL 18 production . ^^^ In contrast , IFN gamma knockout mice developed scattered focal necrosis of the liver with significantly lower levels of serum alanine transferase as well as drastic decreases in TNF alpha , IL 12 , and IL 18 production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both lipopolysaccharide and TNF alpha synergize with IFN gamma in the expression of iNOS with subsequent production of nitric oxide . ^^^ In this study , we found that IL 4 also downregulated iNOS and nitric oxide expression induced by IFN gamma and TNF alpha and in mouse macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Plasma cytokine ( TNF alpha , IL 1beta , IL 6 , IFN gamma ) production peaked for all monkeys 11 days after inoculation , concomitantly with peaks of parasitemia . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Antisera to IFN gamma , TNF alpha or IL 12 during IL 12 egg immunization partly restored granuloma size and fibrosis following infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A consistent pattern in disease states , whether it be human or mouse , is an exaggerated Th 1 type response with excess local production of IFN gamma and TNF alpha , and its association with tissue injury . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OBJECTIVES : To investigate the regulatory roles of interleukin 1 beta ( IL 1 beta ) , tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) or transforming growth factor beta 1 ( TGF beta 1 ) on hyaluronan ( HA ) synthesis by human fibroblastic synovial lining cells . ^^^ METHODS : Concentrations of HA in culture supernatants of fibroblastic synovial lining cell line ( RAMAK 1 cell line ) with or without stimulation by IL 1 beta , TNF alpha , IFN gamma or TGF beta 1 were measured by sandwich binding protein assay . ^^^ RESULTS : HA synthesis by the cells was not significantly augmented by TNF alpha or by IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These include cytokines such as tumor necrosis factor alpha ( TNF alpha ) , interleukin 6 ( IL 6 ) , interferon gamma ( IFN gamma ) and leukemia inhibitory factor ( LIF ) , as well as tumor derived factors such as lipid mobilizing factor ( LMF ) and protein mobilizing factor ( PMF ) , which can directly mobilize fatty acids and amino acids from adipose tissue and skeletal muscle respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The proinflammatory cytokines IL 1 , IL 6 , TNF alpha , and IFN gamma had little or no effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma and IFN beta were potent inhibitors of CMV replication in RPE cells , while TNF alpha , IL 1beta , or TGF beta 2 did not affect viral replication . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Measurement of cytokine production by IRBP stimulated splenocytes in vitro showed significantly decreased TNF alpha for IFN alpha / beta treated rats compared to control , but no difference for IFN gamma , IL 2 , IL 4 , and IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
While a pathogenic role for tumor necrosis factor ( TNF ) has been established , we asked whether a disruption of interferon gamma ( IFN gamma ) signaling would modulate CM . ^^^ In the absence of IFN gamma signaling , ICAM 1 and TNF up regulation is reduced ; hence , PbA infection fails to cause fatal CM . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we examined the expression of the mRNAs for TNF alpha , IFN gamma , IL 1beta , IL 2 , and IL 2 receptor ( IL 2R ) by reverse transcription polymerase chain reaction ( RT PCR ) using bovine specific primers . ^^^ These results are the first to describe mRNA expression for TNF alpha , IFN gamma , and IL 1beta in the bovine corpus luteum , and support a role for these cytokines in luteal function and regression . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
C 6 glioma cells endogenously express both 5 HT2A receptors and inducible nitric oxide synthase ( iNOS ) . iNOS can be induced by transcriptional activation to produce nitric oxide ( NO ) in response to a challenge with the pro inflammatory cytokines TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the role of gelsolin and its cleavage product in apoptosis of vascular smooth muscle cells ( SMC ) induced by the proinflammatory cytokines interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^ Treatment with a combination of IFN gamma and TNF alpha reduced viability of SMC in a time and concentration dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lesions show a mixed inflammatory infiltrate partly localized in the adventitia where there are T CD4+ lymphocytes secreting 2 2 and IFN gamma and also macrophages expressing TGF beta 1 , IL 6 and IL 1 beta , and partly situated in the interior part of the wall , around the internal elastic lamina , and mostly made of macrophages and giant cells which produce TNF , collagenase and nitric oxide that are responsible for destruction of the wall . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Proinflammatory ( IL 6 , IL 1 beta and TNF alpha ) and antiinflammatory ( IL 10 ) cytokine and lymphokine ( IL 2 , IL 4 and IFN gamma ) polymerase chain reaction ( PCR ) ratios were monitored in unmanipulated peripheral blood mononuclear cells ( PBMCs ) during acute infection by using a semiquantitative reverse transcription ( RT ) PCR method . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Exposure of hematopoietic progenitor cells ( HPC ) from mice and humans with Fanconi anemia group C ( FAC ) to interferon gamma ( IFN gamma ) or tumor necrosis factor alpha ( TNF alpha ) at doses too low to inhibit growth of normal HPC induces profound apoptotic responses . ^^^ Because the IFN gamma hypersensitivity of cells lacking the FAC protein is mediated , in part , through priming of the Fas pathway , and because several other members of this family are capable of inducing apoptosis either alone or in concert with each other , we tested the hypothesis that IFN gamma induces increased expression of members of the TNF receptor ( TNFR ) superfamily in cells nullizygous for the FAC gene . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
By use of the human epithelial cell line HT 29m3 , we show that IFN gamma , TNF alpha and IL 4 activate transcription of the SC gene . ^^^ IFN gamma and TNF alpha , but not IL 4 , also up regulated expression of HLA class 1 genes . ^^^ Nuclear run on experiments showed that the transcription rate of HLA class 1 genes nearly peaked after only 30 min of IFN gamma or TNF alpha stimulation , whereas the SC transcription rate did not peak until after 20 36 h of IFN gamma , TNF alpha or IL 4 stimulation . ^^^ Gel electrophoresis mobility shift assays demonstrated binding of nuclear proteins from cytokine stimulated HT 29 cells to consensus elements in the promoter of the SC gene , involving the binding site for the nuclear factor kappaB p 50 subunit after TNF alpha stimulation , and IFN stimulated response element after IFN gamma stimulation ( and weakly after TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The role of TNF in MTX mediated effects on cytokine production was further underlined by the finding that MTX effects on IFN gamma production were augmented in TNF transgenic mice but abrogated in mice in which the TNF alpha gene had been inactivated by homologous recombination . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These clinical effects were associated with dose dependent down modulation of PNS antigen induced T and B cell responses and with suppression of the proinflammatory cytokines IL 12 , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) mRNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When introduced individually to cultures of CG 4 cells , the cytokines , i . e . , tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 ( IL 1 ) , and interferon gamma ( IFN gamma ) , had either minimal ( TNF alpha ) or no ( IL 1 and IFN gamma ) detectable stimulatory effect on the production of nitric oxide . ^^^ However , combinations of these factors , in particular , TNF alpha plus IFN gamma , elicited a strong enhancement of nitric oxide synthesis and , as revealed by western blot and RT PCR analysis , the expression of iNOS . ^^^ In contrast , IFN gamma neither activated on its own nor enhanced the activation of p 38 MAPK in response to TNF alpha and IL 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , the expressions of mRNAs for IL 2 , IL 4 , IL 5 , IFN gamma , and TNF alpha in peripheral blood T lymphocytes and the expression of IL 5 mRNA in peripheral blood eosinophils were detected before and during therapy , which were unchanged by therapy with IPD 1151T . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We report that the strong gammadelta T cell response to TUBAg is characterized by the rapid and selective production of the Th1 / proinflammatory cytokines IFN gamma and TNF alpha in responder HIV infected donors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Recombinant IL 1alpha , IL 2 , IL 6 , TNF alpha and IFN gamma had no influence . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , if CD 4 cells act to reject by recruiting and / or activating macrophages then this was not dependent on CR 3 , IFN gamma or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Urine of patients showed increased levels of IL 1beta , IL 2 , IL 6 , tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) and macrophage colony stimulating factor ( M CSF ) after BCG instillation . ^^^ Levels of IL 2 and IFN gamma in the serum also increased after BCG instillation , but IL 1beta , IL 6 , TNF alpha and M CSF were not detectable . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Therefore , we analysed the CD40L induced MMP production by these fibroblasts in the presence of cytokines that are increased in periodontal lesions , such as IL 1beta , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) . ^^^ This down regulatory effect of CD 40 engagement on MMP 1 and MMP 3 production by gingival fibroblasts was also present when MMP production was up regulated by IL 1beta and TNF alpha or down regulated by IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To determine whether differences in such infection outcome are associated with a particular pattern of cytokine response , a study was undertaken to investigate the serum levels of TNF alpha , IFN gamma , IL 4 , IL 6 , and IL 10 by using an enzyme linked immunosorbent assay . ^^^ Infection with the TUL strain of T . cruzi was accompanied by a significant increase of TNF alpha serum concentrations at day 5 p . i . , and detectable amounts of IFN gamma by day 15 p . i . , which were exclusively recorded in this group . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In lungs of tumor bearing mice treated with matched TIL + rIL 2 , significant increases in the percentages of IFN gamma , GM CSF and tumor necrosis factor alpha ( TNF alpha ) positive cells were detected , as well as of macrophages , natural killer ( NK ) cells and dendritic cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The relationship between stem cell mobilization with granulocyte colony stimulating factor ( G CSF ) and the endogenous production of interleukin 8 ( IL 8 ) , macrophage inflammatory protein 1alpha ( MIP 1alpha ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) was studied in normal donors for allogeneic peripheral blood stem cell ( PBSC ) transplantation . ^^^ In contrast , before treatment , the average levels of MIP 1alpha , TNF alpha , and IFN gamma were 70 . 1 , 4 . 03 , and 3 . 84 pg / mL , respectively , and no significant changes in the levels of these cytokines were observed during G CSF treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cadherin 5 redistribution at sites of TNF alpha and IFN gamma induced permeability in mesenteric venules . ^^^ The response of the endothelial permeability barrier in microvascular networks of the rat mesentery to perfused immune inflammatory cytokines tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) was examined . ^^^ TNF alpha ( 12 . 5 U / ml ) treatment did not change albumin permeability , but in combination with IFN gamma ( 20 U / ml ) , there was a marked increase in the number of sites of extravascular albumin in postcapillary venules . ^^^ These results show that the combination of TNF alpha and IFN gamma induces an endothelial phenotype with focal loss of cadherin 5 intercellular adhesion , which , in part , facilitates passage of blood macromolecules and cells to the interstitium . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 12 induced increases in plasma concentrations of IL 15 , IL 18 , and interferon gamma ( IFN gamma ) , plus a marked antiinflammatory cytokine response ( IL 10 , soluble tumor necrosis factor [ TNF ] receptors , IL 1 receptor antagonist ) and secretion of alpha chemokines ( IL 8 , IFN gamma inducible protein 10 ) and beta chemokines ( monocyte chemoattractant protein 1 , macrophage inflammatory protein 1beta ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunocytochemistry was used to monitor the dynamics of cytokine ( interleukin 2 [ IL 2 ] , IL 4 , IL 6 , IL 10 , gamma interferon [ IFN gamma ] , and tumor necrosis factor alpha [ TNF alpha ] ) and viral antigen production in the TG and the adjacent central nervous system on days 1 to 4 , 6 , 7 , and 10 after irradiation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lymphocyte transformation and cytokine production , that is , interleukin 6 ( IL 6 ) , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) , were used to evaluate the immunological activities of MMA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PURPOSE : To determine whether nuclear transcription factor kappaB ( NF kappaB ) is activated in human retinal pigment epithelial ( hRPE ) cells in response to interleukin 1beta ( IL 1beta ) , tumor necrosis factor alpha ( TNF alpha ) , or interferon gamma ( IFN gamma ) alone or in combination and if so , whether expression of proinflammatory genes induced by these agents can be blocked by a proteasome inhibitor , MG 132 , which inhibits the degradation of 1 kappaB , an NF kappaB inhibitor , thereby preventing nuclear translocation of NF kappaB . ^^^ METHODS : Cultured hRPE were pretreated for 60 minutes with medium alone or medium containing the proteasome inhibitor MG 132 ( 20 microM ) and then exposed to TNF alpha ( 1 . 1 10 10 ( 3 ) U / ml ) , IL 1beta ( 5 U / ml ) , or IFN gamma ( 7 . 5 10 10 ( 3 ) U / ml ) alone or in combination ( TII ) . ^^^ RESULTS : TNF alpha , IL 1beta , and TII but not IFN gamma alone caused degradation of 1 kappaB , Rel A nuclear translocation , and increased NF kappaB DNA binding activity , effects that were blocked by pretreatment with MG 132 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma mediated control of Coxiella burnetii survival in monocytes : the role of cell apoptosis and TNF . ^^^ TNF was involved in IFN gamma induced killing of C . burnetii and cell death . ^^^ IFN gamma induced mRNA expression and sustained secretion of TNF . ^^^ Neutralizing Abs to TNF as well as Abs directed against TNF receptors 1 and 2 , significantly prevented IFN gamma dependent killing of C . burnetii and cell death . ^^^ These results suggest that IFN gamma promotes the killing of C . burnetii in monocytes through an apoptotic mechanism mediated in part by TNF . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Synthesis of pIgR is up regulated by the proinflammatory cytokines TNF alpha , IFN gamma , and IL 1 in HT 29 human colon carcinoma cells . ^^^ We previously reported that IFN gamma and TNF alpha induce production of the transcription factor IFN regulatory factor 1 ( IRF 1 ) in HT 29 cells and that IRF 1 binds to an element in exon 1 of the PIGR gene . ^^^ We now report that levels of IRF 1 and pIgR mRNA are coordinately regulated in HT 29 cells by TNF alpha , IFN gamma , and IL 1beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of IL 10 mRNA was higher and IL 4 mRNA was lower in the brains of infected SJL than in BALB / c mice , while expression of the mRNAs of IL 6 , IL 1beta , TNFalpha , and the Th 1 cytokines IL 2 , IL 12 , and IFN gamma was similar . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We demonstrate here that primary human glial cells were able to produce alpha and beta chemokines ( IL 8 > growth related protein alpha ( GROalpha ) > > RANTES > microphage inflammatory protein ( MIP ) 1alpha and MIP 1beta ) in parallel to PGs ( PGE 2 and PGF2alpha ) after proinflammatory cytokine stimulation : TNF alpha + IL 1beta induced all except RANTES , which was induced by TNF alpha + IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , in the same tissue , no differences between patient groups were found when IFN gamma , TNF alpha , IL 4 , IL 5 , and IL 12 mRNA expression were examined . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cutting edge : chronic intestinal inflammation in STAT 4 transgenic mice : characterization of disease and adoptive transfer by TNF plus IFN gamma producing CD4+ T cells that respond to bacterial antigens . ^^^ The latter cells produced predominantly TNF and IFN gamma but not IL 4 upon activation with alphaCD3 / CD28 or autologous bacterial Ags , consistent with a Th 1 type cell response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Fas ligand and Fas are expressed constitutively in human astrocytes and the expression increases with IL 1 , IL 6 , TNF alpha , or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This immediate , IL 12 mediated shift in innate inflammatory phenotype was correlated with a significant reduction of lung concentrations of the neutrophil chemoattractant macrophage inflammatory protein ( MIP ) 2 ( putative murine homologue of human IL 8 ) , monocyte chemotactic protein 1 , and TNF alpha ; and a reduction in MIP 1alpha and IFN gamma , at high dose infection only , and IL 12 independent IL 10 levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Two cytokines induced in association with colitis in these mice , TNF alpha and IFN gamma , increased the expression of KC mRNA and protein in murine epithelial cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These include IFN gamma , IL 12 , TNF alpha , and IL 6 , which are important in the control of intracellular bacterial infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) and gamma interferon ( IFN gamma ) cooperate during a variety of biological responses and ultimately synergistically enhance the expression of genes involved in immune and inflammatory responses . ^^^ Recently , we demonstrated that IFN gamma can significantly potentiate TNF alpha induced nuclear factor ( NF ) kappaB nuclear translocation in neuronal derived and endothelial cell lines . ^^^ The double stranded RNA dependent kinase PKR is IFN inducible and has been implicated in the activation of NF kappaB ; therefore , we examined the possibility that PKR may play a role in the synergistic activation of NF kappaB during TNF alpha / IFN gamma cotreatment . ^^^ The PKR inhibitor 2 aminopurine ( 2 AP ) inhibited TNF alpha / IFN gamma induced NF kappaB nuclear translocation in neuronal derived cells but not in endothelial cells . ^^^ The induced degradation of IkappaBbeta , which is normally observed upon TNF alpha / IFN gamma cotreatment , was blocked completely by 2 AP in neuronal derived cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cationic lipid mediated gene transfer to the mouse lung induces a dose dependent inflammatory response that is characterized by an influx of leukocytes and elevated levels of the cytokines interleukin 6 ( IL 6 ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) . ^^^ We report here that complexes of cationic lipid GL 67 and unmethylated pDNA ( pCF 1 CAT ) instilled into the lungs of BALB / c mice induced highly elevated levels of the cytokines TNF alpha , IFN gamma , IL 6 , and IL 12 in the bronchoalveolar lavage fluids ( BALF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our findings show that synergistic enhancement of CTL activity and IFN gamma production could play an important role in the antitumor effect of combination therapy using TNF gene transduced cancer vaccine and rIL 12 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The mutant mice are highly susceptible to endotoxin shock with increased production of inflammatory cytokines such as TNF alpha , IL 1 , IFN gamma , and IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These results suggest that ( 1 ) the specific interaction of P . carinii organisms with IFN gamma primed AMs triggers the production of RNI , ( 2 ) RNI are toxic to P . carinii , and ( 3 ) TNF alpha likely plays a central role in mediating P . carinii killing by IFN gamma stimulated AMs . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The data reported in this study show that preincubation of resting T cells from the human CD4+ clone SP B 21 ( and clone TA 23 . 6 ) with IL 10 strongly enhances their capacity to further produce IL 2 , interferon gamma ( IFN gamma ) , IL 4 and tumour necrosis factor alpha ( TNF alpha ) after subsequent activation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) , potent inflammatory cytokines , are released by macrophages during endotoxin shock . ^^^ The expression of E selectin , measured using the dual radiolabeled monoclonal antibody ( mAb ) technique , was monitored in different tissues of endotoxin challenged wild type and IFN gamma deficient mice receiving a mAb to TNF alpha ( TN 3 ) . ^^^ These findings indicate that TNF alpha is essential for achievement of maximal E selectin expression in most vascular beds during endotoxemia , whereas the contribution of IFN gamma is largely confined to the small intestine . . ^^^ Tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) , potent inflammatory cytokines , are released by macrophages during endotoxin shock . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The KO mice also had a strongly polarized Th 2 immune response as judged by elevated levels in the lungs of messenger RNA ( mRNA ) transcripts for IL 4 , IL 5 , IL 6 , and IL 13 , but decreased levels of mRNA for interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^ In contrast , wild type C57BL / 6 or KO mice treated with recombinant IL 12 had a polarized Th 1 phenotype with elevated levels of IFN gamma and TNF alpha mRNA in the lungs , and an airway cell population that secreted abundant IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma potentiates the release of TNF alpha and MIP 1alpha by alveolar macrophages during allergic reactions . ^^^ The production of interferons ( IFNs ) is well known to limit viral spread , but IFN gamma can also prime alveolar macrophages to release more inflammatory cytokines , such as tumor necrosis factor alpha ( TNF alpha ) and macrophage inflammatory protein 1alpha ( MIP 1alpha ) . ^^^ Treatment with IFN gamma prior to stimulation with IgE / anti IgE showed a time and concentration dependent increase of TNF alpha release . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These studies demonstrate that lymphocytes and monocytes in stem cell products are abnormally activated , expressing significantly higher levels of interleukin ( IL ) 2 , 4 and 10 , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) , but not IL 8 , as compared to normal peripheral blood mononuclear cells ( PBMC ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To address this issue , we measured the proliferative response , variation of T cell subpopulations ( CD4+ , CD8+ , TCR 5 delta 2 and TCR 5 beta 8 T cells ) and the cytokine profile ( IL 1 beta , IL 2 , IL 4 , IL 6 , IL 10 , TNF alpha , IFN gamma ) after MT stimulation of peripheral blood mononuclear cells ( PBMC ) in a patient with concomitant CML and active pulmonary tuberculosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , the IL 12 induced antimetastatic effect was not mediated by interferon gamma ( IFN gamma ) or tumor necrosis factor alpha ( TNF alpha ) as shown with IFN gamma receptor and TNF alpha knockout mice , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Reverse transcription PCR analysis of the lung tissues of MAC infected mice at weeks 4 and 8 after infection revealed a marked increase in the levels of tumor necrosis factor alpha , gamma interferon ( IFN gamma ) , IL 10 , and TGF beta mRNAs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neither the pro inflammatory cytokines interleukin 2 ( IL 2 ) , TNF alpha nor LPS alone or in combination with interferon gamma ( IFN gamma ) had any effect on LT beta mRNA expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine levels ( IL 1beta , IL 2 , IL 6 , TNF alpha , IFN gamma ) and sIL 2R of individual patients before , during ( 3 months ) and after ( 6 months ) therapy were evaluated and correlated to clinical response according to UICC criteria ( responder patients partial or Complete Response versus non responder patients Stable / Progression ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The production and roles of endogenous tumor necrosis factor alpha ( TNF alpha ) and gamma interferon ( IFN gamma ) in the infection of Corynebacterium ( C . ) pseudotuberculosis were investigated in mice . ^^^ The maximum levels of TNF alpha and IFN gamma were detected on day 4 after infection . ^^^ The administration of anti TNF alpha monoclonal antibody ( mAb ) as well as anti IFN gamma mAb increased bacterial proliferation in the organs , leading to the death of infected mice , but anti IFN gamma mAb showed a less marked effect than anti TNF alpha mAb . ^^^ The suppressive effect of anti TNF alpha and anti IFN gamma mAbs on anticorynebacterial resistance was augmented by the simultaneous administration of these antibodies . ^^^ Histologically , many microabscesses , severe follicular swelling and lymphocyte destruction were observed in mice treated with anti TNF alpha or anti IFN gamma mAb . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : IFN gamma , TNF alpha , and IL 2 production by T cells decreased significantly by 48 . 3 % , 36 . 6 % , and 36 . 8 % , respectively , on postoperative day 1 after CCE , but not after LCE . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here , we show that anti CD 47 , anti beta 3 mAb and Vn , but not Fn , inhibit sCD 23 mediated proinflammatory function ( TNF alpha , IL 12 , and IFN gamma release ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Depletion of either CD 4 ( + ) or CD 8 ( + ) T cells may prevent beta cell destruction by decreasing IFN gamma and IL 10 production in islets and increasing IL 4 and TNF alpha production systemically . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Examination of proinflammatory cytokine , chemokine , and Mt mRNA in the lung revealed increases for messages encoding IL 1 alpha , IL 1 beta , IL 6 , IFN gamma , TNF alpha , Eotaxin , MIP 1 alpha , MIP 1 beta , MIP 2 , Mt , and IP 10 , while messages encoding IL 12 , IL 10 , IFN beta , Ltn , MCP 1 , TGF beta 1 + 2 , and RANTES were unchanged from those of sham exposed mice 2 h postexposure . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OBJECTIVE : Utilizing ovarian cancer cell lines , we examined the effect of IFN gamma on each type of TNF receptor . ^^^ RESULTS : IFN gamma treatment resulted in an increase in TNF receptors in the cell lines A 2780 and Caov 3 ( P < 0 . 001 ) , but not SK OV 3 . ^^^ In Caov 3 and SK OV 3 cells a synergistic increase in TNF alpha mediated cytolysis was seen when cells were pretreated with IFN gamma . ^^^ CONCLUSIONS : TNF receptor modulation by IFN gamma appears to be unique to individual cell lines . ^^^ The TR 60 TNF receptor plays a central role in the synergistic cytolytic effects of IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Psoriasis , a disorder characterized by disfiguring skin lesions and elevated levels of activated CD4+ T helper lymphocytes in both peripheral blood and lesional tissue , exhibits a profile of cytokine expression that includes high levels of TNF alpha and IFN gamma , with low IL 5 and IL 10 , indicating that immunologically , the pathogenesis of the disease is Th 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The combination of TNF alpha and IFN gamma was the minimal stimulation required for regulated on activation , normal T cell expressed and secreted ( RANTES ) and monocyte chemoattractant protein ( MCP 1 ) mRNA expression and secretion by HT 29 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The 3 ' noncoding region contains as many as five potential mRNA destabilization signals . mLARC was strongly and transiently induced in the murine monocytoid cell line J 774 by lipopolysaccharide ( LPS ) but not by cytokines such as TNF alpha , IFN gamma , IL 1beta or IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Like MCP 3 , MCP 4 mRNA expression in dermal fibroblasts is upregulated by TNF alpha , IL 1alpha , IFN gamma or IL 4 and differs from RANTES and eotaxin mRNA expression in its response to IFN gamma and / or IL 4 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum nitrite / nitrate , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) concentrations increased significantly in group 2 rats after transplantation but were significantly lower in groups 1 , 3 , and 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Modifications of IFN alpha , TNF alpha and IFN gamma serum levels in children vaccinated against measles . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : In control PBMC , PMA + 1 stimulation significantly increased the percentage of CD3+ cells expressing IL 2 , IFN gamma , TNF alpha , IL 4 and IL 10 , as expected . ^^^ However , although mitogen stimulation significantly enhanced the percentage of the classical Th 1 cytokines ( IL 2 , IFN gamma and TNF alpha ) in the low flux HD PBMC , it had no effect on CD3+ IL 2 or CD3+ TNF alpha producing cells in the PD group . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PA significantly elevated Th 1 cytokines , e . g . , IFN gamma , TNF alpha , and IL 1alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast to the cecal ligation and puncture model of peritonitis , survival of mice following CASP surgery is dependent on IFN gamma , but independent of tumor necrosis factor ( TNF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Eosinophils and mRNA positive cells ( in situ hybridization for IL 2 , IL 3 , IL 4 , IL 5 , IL 6 , IL 8 , IL 10 , IL 13 , IFN gamma , RANTES , and TNF alpha ) were assessed in the biopsy specimens . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The findings presented herein demonstrate that , under either basal conditions or tumor necrosis factor alpha ( TNF alpha ) and / or interferon gamma ( IFN gamma ) stimulated conditions , a consistent pattern of differences in the secretion of IL 8 and MCP 1 between CF and non CF epithelial cells was not observed . ^^^ In contrast , CF epithelial cells expressed no detectable RANTES protein or mRNA under basal conditions or when stimulated with TNF alpha and / or IFN gamma ( P < / = 0 . 05 ) , unlike their non CF counterparts . ^^^ Correction of the CF transmembrane conductance regulator ( CFTR ) defect in CF airway epithelial cells restored the induction of RANTES protein and mRNA by TNF alpha in combination with IFN gamma ( P < / = 0 . 05 ) but had little effect on IL 8 or MCP 1 production compared with mock controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : G4 . 18 treatment modulated T cell receptor / CD3 and CD 2 and depleted T cells by < 20 % but did not induce activation surface markers . mRNA for interleukin ( IL ) 2 , interferon ( IFN ) gamma , tumor necrosis factor ( TNF ) alpha , and IL 4 in the lymph node , spleen , and thymus was not increased , and IFN gamma mRNA was reduced . ^^^ G4 . 18 treated allograft recipients had no induction of mRNA for IL 2 , IFN gamma , TNF alpha , TNF beta , IL 4 , IL 5 , IL 10 , perforin , and granzyme A & B in the spleen or grafts , with levels similar to those in isografts . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
None of the osteoblast like cells expressed nNOS , however , and iNOS was produced only by hOB cells after stimulation with the cytokines IL 1beta , TNF alpha , and IFN gamma . ^^^ In contrast , IL 1beta , TNF alpha , and IFN gamma exerted growth inhibiting and differentiation inducing effects on osteoblasts that were partly NO dependent , indicating that NO may act predominantly as a modulator of cytokine induced effects on osteoblast function . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , TRANCE induction is significantly suppressed when cells are stimulated in the presence of IL 4 , but is not modified in the presence of IFN alpha , IFN gamma , TGF beta , TNF alpha , or IL 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Their lymph node cells and splenocytes produced a distinct pattern of cytokines in response to IRBP : reduced IFN gamma and IL 12 p 40 , but unchanged levels of TNF alpha , IL 4 , IL 5 , and IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using fibroblasts from RelA ( p 65 ) deficient mice generated by gene targeting , we have investigated the role of this subunit of NF kappaB in gene activation by microbial lipopolysaccharide , tumor necrosis factor alpha , and in possible synergism with the IFN gamma signaling pathway . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After injection of LPS and D gal , CD 7 deficient mice had decreased serum IFN gamma and tumor necrosis factor ( TNF ) alpha levels compared with control C57BL / 6 mice ( P < 0 . 001 ) . ^^^ Steady state mRNA levels for IFN gamma and TNF alpha in liver tissue were also significantly decreased in CD 7 deficient mice compared with controls ( P < 0 . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , the immune response after combination therapy was characterized by substantially augmented IFN gamma and TNF alpha production and strong CTL activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using in situ hybridization and immunohistochemistry the induction of potential cytokines [ interleukin 1beta ( IL 1beta ) , IL 4 , IL 6 , IL 10 , tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) and transforming growth factor beta ( TGF beta ) ] in human embryonic forebrain cells at weeks 5 , 7 and 10 of gestation was studied . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
During the period of HSV 1 replication , macrophages and gammadelta TCR+ T lymphocytes infiltrate the TG , and TNF alpha , IFN gamma , the inducible nitric oxide synthase ( iNOS ) enzyme , and IL 12 are expressed . ^^^ TNF alpha , IFN gamma , and the iNOS product nitric oxide ( NO ) all inhibit HSV 1 replication in vitro . ^^^ Macrophage and gammadelta TCR+ T cell depletion studies demonstrated that macrophages are the main source of TNF alpha and iNOS , whereas gammadelta TCR+ T cells produce IFN gamma . ^^^ Macrophage depletion , aminoguanidine inhibition of iNOS , and neutralization of TNF alpha or IFN gamma all individually and synergistically increased HSV 1 titers in the TG after HSV 1 corneal infection . ^^^ Moreover , individually depleting macrophages or neutralizing TNF alpha or IFN gamma markedly reduced the accumulation of both macrophages and gammadelta TCR+ T cells in the TG . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma and tumor necrosis factor alpha ( TNF alpha ) have been shown to be crucial in the pathogenesis of viral hepatitis and in the transient disappearance of hepatitis B virus ( HBV ) from the liver after adoptive transfer of HBV specific cytotoxic T lymphocytes into HBV transgenic mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Enzyme linked immunospot ( ELISPOT ) assays revealed higher levels of interleukin ( IL ) 4 , IL 10 and interferon gamma ( IFN gamma ) secreting cells among DCs than among MNC or T cells obtained from the same blood specimens , while levels of tumour necrosis factor alpha ( TNF alpha ) and IL 6 secreting cells did not differ . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Reverse transcription PCR analysis revealed that Yokuinin increased the mRNA expression of all test cytokines in lung tissues of infected ice at week 8 , in the order transforming growth factor beta ( TGF beta ) > IFN gamma > TNF alpha > IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A quantitative analysis demonstrated higher IFN gamma and TNF alpha mRNA levels in immune mice at 24 h postinoculation . ^^^ In conclusion , immunization with F . tularensis LVS conferred a capability to respond to cutaneous reinfection , with rapid local expression of IL 12 , TNF alpha , and IFN gamma , and this expression was paralleled by containment and mitigation of the infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NO production requires interleukin ( IL ) 12 mediated gamma interferon ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) synthesis as demonstrated by inhibition experiments using neutralizing anti IL 12 , anti IFN gamma , and anti TNF alpha monoclonal antibodies ( MAb ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
HIV infection does not only lead to a decrease of certain cell types ( CD 4 T lymphocytes ) , but also modifies the function of non infected cells such as B lymphocytes and NK cells by upregulating cytokine release of IL 1 , IL 6 , GM CSF , IFN gamma and TNF alpha . ^^^ Using ELISA , RIA and IRMA techniques we analysed the influence of seven cytokines ( IL 1beta , IL 6 , TNF alpha , GM CSF , IFN alpha , IFN beta , IFN gamma ) and the soluble IL 2 receptor ( sIL 2R ) on the growth of eight different KS derived cell lines compared with eight fibroblast cell lines , established from skin biopsies of HIV positive individuals . ^^^ The KS derived cell culture medium demonstrated significantly higher concentrations than the fibroblast cell lines in view of the following cytokines : sIL 2R , IL 1beta , IL 6 , TNF alpha , GM CSF , IFN gamma ( p < 0 . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Analysis of selected cytokines 1 day after initiation of BLM induced pulmonary fibrosis indicated that the levels of TNF alpha and IFN gamma appeared to segregate with fibrosis in both the SCID and wild type mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A prospective study in which IL 1 , IL 2 , IL 6 , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) have been determined in 66 newly diagnosed lung cancer patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore other studies have suggested that the lymphokine , interferon gamma ( IFN gamma , type 2 interferon or immune interferon ) , produces fever and enhances thermogenesis , body weight loss , and skeletal muscle depletion in rodents in a manner similar to that seen with TNF and IL 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Its induction depends among others on cytokines such as TNF alpha , IFN gamma , IL 1 or on retinoic acid ( RA ) , a key regulator of epidermal homeostasis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Monomorphic HLA class 1 ( non A , non B ) expression on Ewing ' s tumor cell lines , modulation by TNF alpha and IFN gamma . ^^^ HLA Ib molecules encoded by the HLA E , F or G loci with a molecular mass of less than 44 kDa were not detected in lysates of either constitutive or TNF alpha plus IFN gamma treated ET cells . ^^^ By contrast , mRNA specific for HLA A , B , C was negative in SK ES 1 but TNF alpha plus IFN gamma reconstituted HLA A , B , C transcription in this cell line . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , TNF alpha was downregulated and could not be stimulated by LPS and / or IFN gamma , reflecting the phenomenon of desensitization . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum levels of tumor necrosis factor alpha ( TNF alpha ) , interleukin 1alpha ( IL 1alpha ) , and interferon gamma ( IFN gamma ) were estimated by conventional ELISA kits in 60 , 42 , and 58 Thai patients , respectively , with beta ( o ) thalassemia HbE and found to be above the normal range in 13 % , 21 % , and 33 % of the patients , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The supernatants of Sendai virus infected hPBL contained biologically active IFN alphan 3 , significant amounts of immunogenic IFN gamma , monokines ( IL 1alpha , IL beta , TNF alpha ) , lymphokines ( IL 6 , TNF beta ) , growth factor ( PDGF AB ) and small concentrations of IL 2 and GM CSF . ^^^ The analysis of the influence of the Sendai virus inactivation by lowering pH 2 . 0 on the cytokine concentrations showed that IL 1alpha , TNF alpha , TNF beta and IFN gamma are susceptible to acid conditions , while IFN alphan 3 , IL 1beta , IL 6 and IL 2 concentrations remained unchanged . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our findings demonstrate that HepG 2 cells express mRNAs for interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) , transforming growth factor beta ( TGF beta ) , macrophage colony stimulating factor ( M CSF ) , oncostatin M ( OSM ) , intercellular adhesion molecule ( ICAM 1 ) , interleukin 4 ( IL 4 ) , IL 5 , IL 7 , IL 10 , IL 11 , IL 12 and IL 6 receptor ( IL 6R ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the expression of Fas antigen ( CD 95 ) in the pure erythroid cell line AS E 2 in the presence and absence of interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^ TNF alpha induced apoptosis in AS E 2 cells , whereas IFN gamma did not . ^^^ In culture containing no IFN gamma or TNF alpha , AS E 2 cells expressed little Fas antigen . ^^^ However , IFN gamma and IFN gamma and TNF alpha both induced expression of Fas antigen and its mRNA within 24 hours after the stimulation . ^^^ Although AS E 2 cells expressed Fas ligand mRNA , treatment with ZB 4 , an antibody that inhibits Fas mediated cell death , failed to suppress IFN gamma or TNF alpha mediated cytotoxicity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We detected iNOS mRNA by reverse transcriptase polymerase chain reaction in CD34+ human bone marrow cells after 48 hour incubation with interferon gamma ( IFN gamma ) / tumor necrosis factor alpha ( TNF alpha ) and IFN gamma / lipopolysaccharide . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using a human myeloblastic leukemia cell line , EoL 1 , we investigated the effect of interferon gamma ( IFN gamma ) , which differentiates EoL 1 into monocyte / macrophage like cells on Fas antigen ( Fas ) and tumor necrosis factor alpha ( TNF alpha ) induced apoptosis . ^^^ In conclusion , TNF induced apoptosis was mediated preferentially by TNFR 1 , and the anti apoptotic effect of IFN gamma was result from up regulated TNFR 2 in EoL 1 cell line . ^^^ This cell line is a useful model to provide new insights into crosstalk among Fas / FasL , TNF , and IFN gamma mediated signaling . . ^^^ In flow cytometric analysis , EoL 1 expressed two types of tumor necrosis factor receptors ( TNFR 1 and TNFR 2 ) , and the expression of TNFR 2 but not of TNFR 1 was up regulated significantly after the IFN gamma treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using a three cytokine mixture ( TNF alpha , IL 1beta , and IFN gamma ) that could maximally induce both iNOS and sPLA 2 , the increase in these mRNA species reached a maximum by 4 8 h , followed by a decline up to 48 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Other basement membrane derived growth factors such as TGF beta , basic fibroblast growth factor ( bFGF ) , and platelet derived growth factor ( PGDF ) , as well as tumor necrosis factor ( TNF alpha ) , keratinocyte growth factor ( KGH ) , nerve growth factor ( NGF ) and interferon gamma ( IFN gamma ) were inactive . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We show that in vitro long term established OVA and beta gal specific CTL clones release TNF alpha and IFN gamma when incubated with Ag negative Hsp 70 and gp 96 . ^^^ In the absence of antigenic peptides , HSP mediated secretion of TNF alpha and IFN gamma requires cell contact of the APC with the T cell but is not MHC 1 restricted . ^^^ In unprimed animals , these T cells lyse OVA transfected cell lines and produce TNF alpha and IFN gamma after Ag stimulus . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A prominent glucocorticoid response peaks 36 h following murine CMV ( MCMV ) infection , coincident with circulating levels of the cytokines IL 12 , IFN gamma , TNF , and IL 6 , and dependent on IL 6 for maximal release . ^^^ Lack of endogenous glucocorticoids resulted in increases in IL 12 , IFN gamma , TNF , and IL 6 production , as well as in mRNA expression for a wider range of cytokines , also including IL 1 alpha and IL 1 beta . ^^^ TNF , but not IFN gamma , was required for increased lethality in the absence of endogenous hormone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Resistance to the mouse pneumonitis ( MoPn ) strain of Chlamydia trachomatis has been mapped to MHC class 2 restricted , IL 12 dependent CD4+ T cells that secrete a type 1 profile of proinflammatory cytokines , which includes IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found that IFN gamma , but not TNF alpha or IL 1 beta , strongly induced IP 10 , Mig , and 1 TAC mRNA accumulation mainly in NHBEC and that TNF alpha and IL 1 beta synergized with IFN gamma induction in all three cell types . ^^^ High levels of IP 10 protein ( > 800 ng / ml ) were detected in supernatants of IFN gamma / TNF alpha stimulated NHBEC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We confirmed that TNF alpha production is markedly impaired in endotoxin tolerant mice and additionally found 2 to 6 fold decreases in serum IFN gamma in these animals following endotoxin challenge . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In SCID mice with IBD transplanted with IL 12 responsive CD4+ T cells , the colonic lamina propria CD4+ T cells showed a mucosa seeking memory / effector CD45RBlow Th 1 phenotype abundantly producing IFN gamma and TNF alpha . ^^^ In SCID mice transplanted with IL 12 unresponsive STAT 4 / CD4+ T cells , the colonic lamina propria , mesenteric lymph node , and splenic CD4+ T cells produced very little IFN gamma but abundant levels of TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumour necrosis factor alpha , ( TNF alpha ) , not , however , IL 2 , interferon gamma ( IFN gamma ) , or ( alpha melanocyte stimulating hormone ( alpha MSH ) was able to up regulate RANTES and interleukin 8 secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression is only marginally upregulated following stimulation with tumor necrosis factor alpha ( TNF alpha ) or interferon gamma ( IFN gamma ) . ^^^ Similarly , LFA 3 RNA is present constitutively in unstimulated HBMEC with minimal increase after co incubation with TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human synovial endothelial cell ( HSE ) intracellular adhesion molecule 1 ( ICAM 1 ) is upregulated maximally by synergy of tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) . ^^^ ICAM surface staining and ELISA detection reflected similar levels on HUVE and HSE cells , yet mRNA levels were much higher in HSE cells in response to TNF alpha / IFN gamma . ^^^ ICAM 1 immunostaining of HSE cytoplasmic vesicles appeared enhanced in cells treated with TNF alpha / IFN gamma and monensin , an endosomal processing inhibitor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , IFN gamma synergized with TNF alpha to increase RANTES production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
HC , which is known to stimulate HCMV replication in permissive human fibroblast ( HF ) cells , enhanced HCMV gene expression by 2 3 fold in TPA pre or posttreated THP 1 cells , but TNF alpha or IFN gamma had little effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Whole blood was stimulated in vitro using mycobacterial antigens ( purified protein derivative [ PPD ] and culture filtrate proteins [ CFP ] ) . ' Type 1 ' cytokines ( gamma interferon [ IFN gamma ] and interleukin 2 [ IL 2 ] ) , ' type 2 ' cytokines ( IL 5 and IL 10 ) and tumour necrosis factor alpha ( TNF alpha ) were measured in culture supernatants . ^^^ In association with HIV infection , IFN gamma responses to mycobacterial antigens were profoundly impaired ( odds ratio [ OR ] 0 . 10 for PPD , 0 . 06 for CFP , P < or =0 . 001 ) , but production of IL 2 , IL 5 and TNF alpha was relatively sustained , and IL 10 increased or sustained ( OR 3 . 97 for PPD , P = 0 . 01 , 1 . 14 for CFP , P = 0 . 99 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast to wild type animals , IFN gamma receptor deficient mice were unable to activate their macrophages as evidenced by a lack of major histocompatibility complex ( MHC ) class 2 antigen induction and the absence of an upregulation of tumor necrosis factor ( TNF ) alpha and inducible nitric oxide synthase ( iNOS ) mRNA transcripts , two macrophage effector molecules . ^^^ In conclusion , these studies demonstrate the important role of the cytokines IFN gamma and TNF alpha and their receptors , respectively , for an effective control of T . gondii . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In three patients tumour necrosis factor alpha ( TNF alpha ) , gamma interferon ( IFN gamma ) and interleukin 2 ( IL 2 ) were transiently elevated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Indeed , the protective effects of exogenously administered IL 10 against murine endotoxin lethality have been shown to correlate with its ability to inhibit the LPS induced production of tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NO release was blocked in the presence of IFN gamma antibodies and partially inhibited by TNF alpha antibodies . ^^^ The ability of PSA in inducing the production of IFN gamma and TNF alpha by spleen lymphocytes was confirmed assaying these cytokine levels in the SN . ^^^ On the other hand , spleen cells obtained from mice treated with PSA 24 h earlier , did not produce significant levels of IFN gamma or TNF alpha when incubated in vitro and showed a significantly lower proliferation rate when pulsed in vitro with PSA or Concanavalin A ( ConA ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
As such , we have examined the production of chemokines by human astroglioma cell lines and primary human astrocytes treated with a variety of stimuli , including LPS , TNF alpha , IFN gamma and IL 1beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found that islets deficient in Fas , IFN gamma receptor , or inducible nitric oxide synthase had normal diabetes development ; however , the specific lack of TNF alpha receptor 1 ( p 55 ) afforded islets a profound protection from disease by altering the ability of islet reactive , CD 4 ( + ) T cells to establish insulitis and subsequently destroy islet beta cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Together , these findings suggest that , in concert with bone marrow derived effectors , nonhemopoietic cells can directly mediate , in the absence of endogenous iNOS , IFN gamma and TNF alpha dependent host resistance to intracellular infection . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The mRNA of interleukin 1 beta ( IL 1 beta ) , IL 2 , IL 4 , IL 6 , IL 8 , interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) were expressed on the whole tonsillar tissue of all 5 subjects with recurrent tonsillitis , obstructive sleep apnea syndrome caused by tonsillar hypertrophy or tonsillar focal infection . ^^^ The CD 4 positive cells expressed mRNA of IL 1 beta , IL 2 , IL 4 , IL 8 , IFN gamma and TNF alpha . ^^^ The CD 8 positive cells expressed mRNA of IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using two color flow cytometry , we measured intracellular expression of interleukin 1 alpha ( IL 1 alpha ) , IL 2 , IL 4 , IL 6 , IL 8 , interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) in tonsillar mononuclear cells freshly isolated and stimulated by phorbol myristate acetate ( PMA ) and ionomycin . ^^^ Flow cytometric detection of intracellular cytokines in tonsillar mononuclear cells stimulated with PMA and ionomycin revealed that CD 3 cells produced IL 1 alpha , IL 2 , IL 4 , IL 8 , IFN gamma and TNF alpha , and CD 19 cells produced IL 1 alpha , IL 6 , IL 8 and TFN alpha . ^^^ In CD 3 cells , the number of cells producing IL 2 and TNF alpha were significantly higher than those expressing other cytokines ; and the number of cells producing IFN gamma and IL 8 were significantly higher than those expressing IL 4 and IL 1 alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The modulating effects of proinflammatory cytokines interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) , and immunoregulating cytokines IL 10 and transforming growth factor beta ( TGF beta ) , on anti microbial activity of murine peritoneal macrophages against Mycobacterium avium intracellulare complex . ^^^ We assessed the roles of proinflammatory cytokines IFN gamma and TNF alpha , and immunoregulatory cytokines IL 10 and TGF beta in the modulation of the anti microbial activity of murine peritoneal macrophages against Mycobacterium avium intracellulare complex ( MAIC ) . ^^^ First , both IFN gamma and TNF alpha significantly reduced the bacterial growth in macrophages , indicating that these cytokines participate in up regulation of macrophage anti MAIC function . ^^^ The same result was also obtained for macrophages stimulated with IFN gamma or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After stimulation with HBV antigens only , production of tumour necrosis factor alpha ( TNF alpha ) or IL 10 was observed in all patients , while interferon gamma ( IFN gamma ) was detectable in only 27 patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
During pulmonary infection by live Mycobacterium bovis bacilli Calmette Gurin ( BCG ) in wild type mice , lung macrophages released interleukin 12 ( IL 12 ) , IFN gamma , and tumor necrosis factor alpha ( TNF alpha ) , and expressed surface activation markers . ^^^ However , macrophages in infected IL 12 ( / ) mice released TNF alpha but not IFN gamma and lacked surface activation makers . ^^^ In freshly isolated lung macrophages from naive IL 2 ( / ) mice , mycobacteria alone released TNF alpha but not IFN gamma , whereas exogenously added IL 12 alone released a minimum of IFN gamma . ^^^ Our data reveal that macrophages are a significant source of type 1 cytokines during mycobacterial infection and that both IL 12 and intracellular pathogens are required for the release of IFN gamma but not TNF alpha . ^^^ These findings suggest that macrophages regulate cell mediated immunity by releasing not only IL 12 and TNF alpha but also IFN gamma and that full activation of IFN gamma response in macrophages is tightly regulated . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin 1beta ( IL 1beta ) , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) contribute to the initial stages of the autoimmune destruction of pancreatic beta cells . ^^^ Pancreatic islets isolated from 5 6 week old , pre diabetic female NOD mice were cultured for 48 h before exposure to IL 1beta ( 1 ng / ml ) , TNF alpha ( 5 ng / ml ) , IFN gamma ( 5 ng / ml ) or IGF 1 or 2 ( 100 ng / ml ) for a further 48 h . ^^^ Co incubation with either IGF 1 or 2 was protective against the cytotoxic effects of IL 1beta and TNF alpha , but less so against the effect of IFN gamma . ^^^ These results suggest that IGFs protect pre diabetic NOD mouse islets from the cytotoxic actions of IL 1beta , TNF alpha and IFN gamma by mechanisms which include a reduction in apoptosis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of cultured 3T3 L 1 adipocytes with LPS , tumor necrosis factor alpha ( TNF alpha ) , or interferon gamma ( IFN gamma ) alone failed to induce iNOS activity . ^^^ However , when used in combination , TNF alpha , IFN gamma , and LPS markedly and synergistically increased iNOS activity in these cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To determine the effects of MPL , IFN gamma , TNF alpha , and IL 1 alpha on MHC class 1 expression of B 16 melanoma cells , we evaluated the expression of MHC class 1 molecules with treatments of MPL , IFN gamma , TNF alpha , and IL 1 alpha by flow cytometry . ^^^ The MHC class 1 expression of B 16 melanoma cells treated with IFN gamma , but not TNF alpha or IL 1 alpha , increased in a time dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The results showed that lead ( 250 ppm ) suppressed Th 1 type responses ( delayed type hypersensitivity ( DTH ) , interferon gamma ( IFN gamma ) production ) , enhanced a Th 2 type response ( interleukin 4 ( IL 4 ) production ) , and increased tumor necrosis factor alpha ( TNF alpha ) production from macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Although treatment of PBMCs with interferon alpha ( IFN alpha ) and IFN gamma led to a significant repression of CXCR 4 gene expression , interleukin 1 beta ( IL 1 beta ) , IL 6 , and tumor necrosis factor alpha ( TNF alpha ) had no significant effect on CXCR 4 gene expression in PBMCs . ^^^ In U 937 cells , TNF alpha and phorbol myristate acetate ( PMA ) stimulated CXCR 4 gene transcription ; this effect was reversed with prior treatment of cells with IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Freshly isolated IEL from both groups of calves expressed mRNA for TNF alpha and IFN gamma , but not IL 4 or IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CD 69 , CD 25 , and HLA DR activation antigen expression on CD3+ lymphocytes and relationship to serum TNF alpha , IFN gamma , and sIL 2R levels in aging . ^^^ We further investigated this activation , by measuring early ( CD 69 ) , middle ( CD 25 ) , and late ( HLA DR ) T lymphocyte activation markers on CD3+ lymphocytes , across subjects ( 20 100 years ) together with serum tumor necrosis factor ( TNF alpha ) , interferon gamma ( IFN gamma ) , and soluble interleukin 2 receptor ( sIL 2R ) . ^^^ Serum sIL 2R , TNF alpha , but not IFN gamma , were associated with CD3+ HLA DR+ lymphocytes , TNF alpha with CD8+ CD57+ count and sIL 2R and IFN gamma with the CD3+ CD25+ / CD3+ CD4+ ratio . ^^^ The study confirms age related upregulation of HLA DR on CD3+ lymphocytes , shows some evidence for associated upregulation of CD 25 on CD3+ cells in older subjects , and links serum TNF alpha , IFN gamma , and sIL 2 R to T lymphocyte activation . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Incubation of PBMC with GCS decreased production of IL 12 , IFN gamma , and TNF alpha , and increased production of IL 6 and IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Regulation of intracellular polyamine biosynthesis and transport by NO and cytokines TNF alpha and IFN gamma . ^^^ This study was undertaken to determine the effects NO and the NO synthase ( NOS ) inducing cytokines , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) , exert on polyamine regulation . ^^^ TNF alpha and IFN gamma induction of NO generation resulted in suppressed ODC activity , an effect prevented by the inducible NOS inhibitor L N 6 ( 1 iminoethyl ) lysine ( L NIL ) . ^^^ Administration of NO donors , or TNF alpha and IFN gamma , suppressed [ 3H ] putrescine uptake , thereby preventing transport mediated reestablishment of intracellular polyamine levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Western blot analysis showed a marked reduction in the expression of inflammatory mediators such as cyclooxygenase 2 , IFN gamma , and tumor necrosis factor alpha in arthritic joints of GTP fed mice . ^^^ Histologic and immunohistochemical analysis of the arthritic joints in GTP fed mice demonstrated only marginal joint infiltration by IFN gamma and tumor necrosis factor alpha producing cells as opposed to massive cellular infiltration and fully developed pannus in arthritic joints of non GTP fed mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Sodium butyrate also induces tumor cell sensitization to the apoptotic effect of the combination of TNF alpha and IFN gamma , but it does not modify the level of the FADD / Mort1 adaptator molecule , at the connection between Fas and TNF dependent apoptosis pathways . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Besides LTalpha 3 and IL 2 , these cells also produce high levels of TNF alpha together with significant amounts of IFN gamma and IL 13 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Injection of CD8+ , but not CD4+ , T cells decreased the production of tissue IFN gamma , IL 1beta , and TNF alpha by > 90 % . ^^^ Therapy for 14 days with recombinant human IL 16 significantly inhibited the production of IFN gamma , IL 1beta , and TNF alpha in the synovium . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In astrocytes , TNF alpha enhances IFN gamma induced class 2 MHC transcription . ^^^ Our results demonstrate that TNF alpha does not enhance IFN gamma induced transcriptional activation of the type 4 CIITA promoter , indicating that the enhancing effect of TNF alpha is mediated downstream of CIITA transcription . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Herein , we show that PMN also have the ability to produce MIG and to express 1 TAC mRNA in response to IFN gamma in combination with either TNF alpha or LPS . ^^^ While IFN gamma , alone or in association with agonists such as fMLP , IL 8 , granulocyte ( G ) CSF and granulocyte macrophage ( GM ) CSF , failed to influence MIG , IP 10 , and 1 TAC gene expression , IFN alpha , in combination with TNF alpha , LPS , or IL 1beta , resulted in a considerable induction of IP 10 release by neutrophils . ^^^ Furthermore , IL 10 and IL 4 significantly suppressed the expression of MIG , IP 10 , and 1 TAC mRNA and the extracellular production of MIG and IP 10 in neutrophils stimulated with IFN gamma plus either LPS or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The actions of TNF alpha , IFN gamma , macrophage inflammatory protein 1alpha , IL 1 , IL 1 converting enzyme , Fas , perforin , inducible nitric oxide synthase , and STAT 1 did not contribute to the observed toxicity , nor did B or T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
On the other hand , combined treatment with IL 12 and IL 18 synergistically induced the production of TNF alpha by PEC and this effect was almost completely abrogated by neutralizing anti IFN gamma mAb . ^^^ TNF alpha significantly promoted macrophage NO production and anticryptococcal activity induced by IFN gamma , and furthermore anti TNF alpha mAb partially inhibited these responses . ^^^ Considered together , our results indicated that TNF alpha contributed to the potentiation of IL 12 / IL 18 induced PEC fungicidal activity against C . neoformans through enhancement of IFN gamma induced production of NO by macrophages , but not through increased production of IFN gamma by NK cells . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Blocking PG or NO production increased IL 2 , IFN gamma , and TNF alpha , but not IL 12 production by SC ; IL 10 levels were not reduced . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The patient was monitored for serum IFN , TNF , IL 2 activities and for the ability of peripheral blood leukocytes ( PBL ) to produce IFN alpha / beta , IFN gamma , IL 2 and TNF alpha after in vitro induction . ^^^ Monitoring the cytokine production ability of PBL during IFN gamma therapy revealed an increase in IL 2 , IFN gamma and TNF alpha titers produced upon in vitro induction after 2 weeks of treatment ( 6 injections of rIFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The following assays were performed . ( 1 ) Nitrite produced by rat mesangial cells in primary culture was measured in incubations with tumor necrosis factor alpha ( TNF alpha ) or lipopolysaccharide ( LPS ) , with or without IFN gamma . ^^^ Cells were stimulated with TNF alpha or LPS plus IFN gamma in the presence of NF kappaB inhibitors , herbimycin A ( HerA ) , or the more specific JAK 2 inhibitor AG 490 . ( 2 ) Immunoblotting was performed against the p 65 and p 50 subunits of NF kappaB and iNOS . ( 3 ) Electrophoretic mobility shift assays were performed against NF kappaB in the presence of NF kappaB inhibitors or AG 490 . ( 4 ) iNOS promoter activity was measured in the presence of AG 490 or JAK 2 antisense oligonucleotides . ^^^ TNF alpha or LPS alone did not induce nitrite production , but with IFN gamma these compounds did induce nitrite production . ^^^ Pyrrolidine dithiocarbamate ( PDTC ) , N acetyl L cysteine , dexamethasone ( Dex ) , HerA , and AG 490 partially inhibited LPS / IFN gamma or TNF alpha / IFN gamma induced nitrite production . p 65 was inhibited by the three NF kappaB inhibitors described above , whereas p 50 was not . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The incubation of blood PMN from normal rats in vitro with neutrophil activators ( PAF , leukotriene B 4 , and interleukin 8 ) and different cytokines [ interleukin 1 , tumor necrosis factor alpha , and interferon gamma ( IFN gamma ) ] showed that only IFN gamma was able to induce the production of high amounts of NO . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Bacterial titers in infected cells decreased significantly and in a dose dependent manner following treatment with IL 1beta , TNF alpha , or IFN gamma . ^^^ In addition , IL 4 antagonized in part the bactericidal effect of TNF alpha and IFN gamma . ^^^ Although IL 1beta , TNF alpha , and IFN gamma accelerated killing of intracellular yersiniae the ratio of cells containing bacterial antigen did not differ from that in untreated cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we demonstrate that activated T cells from patients with XHIM produce markedly reduced levels of IFN gamma , fail to induce antigen presenting cells to synthesize IL 12 , and induce greatly reduced levels of TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
On the other hand , proinflammatory cytokines , such as tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) , may be important in the pathogenesis of some neuroimmunological disorders . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Among the 5 cytokine genes , the induction of a marked increase in the mRNA levels of IL 1alpha , IL 1beta , TNF alpha , IFN gamma and M CSF by PSPC and lentinan was observed in the peritoneal exudate cells and splenocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Resident peritoneal macrophages obtained from CBA / J mice were cultured in the presence of recombinant interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and interleukin 10 ( IL 10 ) . ^^^ IFN gamma stimulated nitric oxide ( NO ) formation , IL 10 substantially enhanced the production of NO generated by IFN gamma + TNF alpha , irrespective of the timing and dosing of any of the three cytokines . ^^^ Increased levels of iNOS mRNA after the triple combination of IFN gamma + TNF alpha + IL 10 versus IFN gamma + TNF alpha suggest that the NO enhancing effect is mediated , at the least partially , at the transcriptional level . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 2 was analyzed in a bioassay , while IFN gamma and TNF alpha were measured by ELISA . ^^^ A maximum stimulatory effect on IL 2 production ( 1 . 8 fold increase ) was observed with cisplatin at 5 10 microM while IFN gamma and TNF alpha synthesis and IL 2R density were unaffected . ^^^ However , cisplatin treated cells displayed enhanced IL 2 , IL 2R , IFN gamma and TNF alpha mRNA levels compared to drug free controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Thus , we investigated whether ADCC by MAb 17 1A and BR 55 2 , which recognize TAA on colorectal tumor cells , can be augmented by 3 day incubation with different concentrations of IL 2 , IL 4 , IL 6 , IL 12 , IFN alpha , IFN gamma , GM CSF , M CSF , and TNF alpha . ^^^ We found three reaction patterns with the cytokines tested : ( a ) cytokines , which increase ADCC ( IL 2 , IL 12 , IFN alpha , and IFN gamma , which represent Thl cytokines ) ; ( b ) cytokines with no effect ( GM CSF , M CSF , and TNF alpha ) ; and ( c ) cytokines , which decrease ADCC ( IL 4 and IL 6 , which represent Th 2 cytokines ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We show that , in vitro , interferon gamma ( IFN gamma ) together with interleukin 1beta ( Il 1beta ) and tumor necrosis factor alpha ( TNF alpha ) can induce the expression of CD 23 in glial cells . ^^^ In the SN of PD patients , a significant increase in the density of glial cells expressing TNF alpha , Il 1beta , and IFN gamma was observed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the absence of NO , levels of IL 2 , but not of IFN gamma , TNF alpha , or of any Th 2 regulated cytokines examined , increased significantly . ^^^ However , when AG treatment was brought forward to the early ascending phase of primary parasitemia , significantly increased levels of IFN gamma and TNF alpha , as well as of IL 2 , were observed over those for infected control mice similarly treated with phosphate buffered saline . ^^^ While the immunosuppressive function of NO leads to a downregulation in vivo of production of IL 2 , and indirectly of IFN gamma and TNF alpha , this perceived weakening of the host cell mediated immune response is in part masked by the protective anti malarial effects of NO itself . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Down regulation of TIMP 3 expression is also detected in astrocytes treated with TNF alpha or IFN gamma whereas oncostatin M as well as TNF alpha up regulate TIMP 1 mRNA level . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha , IFN gamma and IL 2 mRNA expression in CIDP sural nerve biopsies . ^^^ In this study in situ hybridisation was used to determine the exact localisation and identity of cells that express TNF alpha , IFN gamma and IL 2 mRNA within the CIDP nerve . ^^^ Sections of these samples were probed with digoxigenin labelled oligoprobes for TNF alpha , IFN gamma and IL 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Injection of P . gingivalis induced the migration of leukocytes into the chambers and increased the intrachamber levels of IFN gamma and TNF alpha . ^^^ There were no significant differences in cell number and IFN gamma levels between the different treatment groups , but the levels of TNF alpha were significantly lower in the isolation stress and cold stress groups compared to control animals . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A combination of IFN gamma and TNF alpha was slightly more effective than the cytokine alone at up regulating CD 40 expression on both cell lines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using the higher bacterial dose , the levels of IL 1beta , TNF alpha and IL 12 remained similar , whereas the IL 6 , IL 8 and IL 10 levels were significantly lower , and IFN gamma levels were significantly higher . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study , we investigated the effect of inflammatory mediators including lipopolysaccharide ( LPS ) , staphylococcal enterotoxin B ( SEB ) , interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and interleukin 1beta ( IL 1beta ) on the adhesion of polymorphonuclear leukocytes or lymphocytes to primary cultured rat hepatocytes , and on the expression of intercellular adhesion molecule 1 ( ICAM 1 ) gene in hepatocytes . ^^^ Both polymorphonuclear leukocyte and lymphocyte adhesion to hepatocytes were enhanced after exposure of hepatocytes to IFN gamma and TNF alpha , but not after exposure to LPS , SEB or IL 1beta . ^^^ The adhesion induced by either IFN gamma or TNF alpha was inhibited by monoclonal antibodies against ICAM 1 or lymphocyte function associated antigen 1 ( LFA 1 ) . ^^^ ICAM 1 mRNA expression was up regulated to a greater extent by incubating hepatocytes with IFN gamma or TNF alpha , and peaked after 12 hr of incubation with TNF alpha and after 24 hr with IFN gamma . ^^^ These results indicate that IFN gamma and TNF alpha induce the expression of ICAM 1 on parenchymal hepatocytes and that the LFA 1 ICAM 1 pathway plays an important role in the interaction between hepatocytes and neutrophils or lymphocytes . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1beta , IL 6 , IFN gamma and TNF alpha in the supernatant were detected by ELISA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
With high sensitivity ELISA systems that measure sub picogram cytokine concentrations , 26 of the 41 patients ( 63 . 4 % ) had at least one of the pro inflammatory Th 1 cytokines ( TNF alpha , IFN gamma and IL 12 ) detected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Secretion of TNF alpha was significantly potentiated by BN 52207 when the PBM were activated by either IFN gamma or LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Kinetic studies demonstrated that virus infected mice had 3 to 50 fold increases over uninfected mice in peak serum TNF , IL 12 , and IFN gamma levels after LPS administration . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
S . mutans stimulated high levels of interleukin 12 ( IL 12 ) , gamma interferon ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) , all of which are associated with inflammation , enhanced monocyte function , and generation of a Th 1 response . ^^^ In contrast , P . endodontalis stimulated production of IL 10 but not of TNF alpha , IL 12 , or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Levels of interleukin 1 ( IL 1 ) , IL 2 , IL 4 , IL 6 , tumor necrosis factor alpha , and gamma interferon ( IFN gamma ) were determined by enzyme linked immunosorbent assay in the sera of infected animals . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We measured proliferation and in vitro production of gamma interferon ( IFN gamma ) , tumor necrosis factor alpha , and interleukin 10 ( IL 10 ) in response to meningococcal antigens by peripheral blood mononuclear cells ( PBMCs ) from children convalescing from meningococcal disease and from controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunity to mycobacterial infection is closely linked to the emergence of T cells that secrete cytokines , gamma interferon ( IFN gamma ) , interleukin 12 ( IL 12 ) , and tumor necrosis factor alpha ( TNF alpha ) , resulting in macrophage activation and recruitment of circulating monocytes to initiate chronic granuloma formation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum interleukin 12 ( IL 12 ) p 70 and gamma interferon ( IFN gamma ) levels were similar but tumor necrosis factor alpha levels were significantly higher in TNFR KO mice than in WT controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The concentration of interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) was increased in LAK supernatant , and the antitumour activity of combined IFN gamma and MMC was synergistic . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Differential role of TNF alpha and IFN gamma in the brain of rats with chronic relapsing autoimmune encephalomyelitis . ^^^ These findings suggest that TNF alpha contributes to the first and second attacks of CR EAE , while IFN gamma is not required for the first attack but is closely related to the relapse of the disease . ^^^ Taken together , differential involvement of TNF alpha and IFN gamma is closely associated with the clinical features of CR EAE . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , IL 4 still induced significant inhibition of the production of TNF alpha and IL 12 from STAT 6 null macrophages that were stimulated with the more physiologically relevant combination of LPS and IFN gamma . ^^^ These data show that STAT 6 is required for the IL 4 mediated inhibition of the production of TNF alpha and IL 12 stimulated by LPS alone , but that IL 4 also activates distinct , STAT 6 independent mechanism ( s ) that inhibit the IFN gamma mediated enhancement of IL 12 and TNF alpha production . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
All the cytokines tested ( IFN alpha , IFN gamma , TNF alpha and IFN gamma + TNF alpha ) increased the expression of HLA class 1 molecules on fibroblasts after 48 h culture , but on TAP deficient cells this expression remained very low as compared to that of normal cells . ^^^ In the presence of IFN alpha , IFN gamma or IFN gamma + TNF alpha , normal target cells became resistant to lysis by autologous NK cells , whereas this effect was much less pronounced in the case of TAP deficient fibroblasts . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Studies with other cytokines ( IL 1beta , IL 6 , TNF alpha , IL 15 ) showed that IL 18 or anti CD 28 treatments were the most efficient inducers of IFN gamma when combined with IL 12 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In L 6 myotubes , glucose uptake stimulated by interferon ( IFN ) gamma or lipopolysaccharides ( LPS ) and a combination of LPS , IFN gamma , and tumor necrosis factor ( TNF ) alpha was inhibited by the antioxidant pyrrolidinedithiocarbamate and potentiated in reduced glutathione ( GSH ) deficient cells . ^^^ Also , the stimulatory effect of LPS and IFN gamma individually , and of a combination of LPS , IFN gamma , and TNF alpha , on glucose uptake was associated with an increased level of intracellular oxidants ( dichlorofluorescein assay ) and loss of intracellular GSH . ^^^ Study of the individual effects of LPS , IFN gamma , and TNF alpha as well as of a combination of the three activators provided evidence against a role of nitric oxide in mediating the stimulatory effect of the above mentioned agents on glucose uptake . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Experiments with in vivo administration of neutralizing anticytokine antibodies revealed that none of interferon ( IFN ) gamma , tumour necrosis factor ( TNF ) alpha and interleukin ( IL ) 1beta were involved in the induction of early activation of LCs and DETCs , while TNF alpha and IL 1beta mediated late activation of LCs , and IFN gamma and IL 1beta mediated that of DETCs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumour necrosis factor ( TNF ) and interferon gamma ( IFN gamma ) , but not interleukin 4 ( IL 4 ) and interleukin 10 ( IL 10 ) , were induced endogenously in mice in relation to the multiplication and clearance of virulent and intermediately virulent strains of R . equi . ^^^ Deaths occurred among mice treated with monoclonal antibodies ( mAbs ) against either TNF or IFN gamma prior to sublethal dose infection with virulent and intermediately virulent strains of R . equi , but not with avirulent R . equi . ^^^ These results suggested that cytokine production depended largely on the virulence levels of R . equi : TNF and IFN gamma were required early during infection with virulent R . equi to limit replication and clearance of bacteria within the organs , but they were not necessary for limiting infection with avirulent R . equi . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The plasma levels of interleukin ( IL ) 1beta , IL 2 , IL 6 , IL 8 , IL 10 , interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) and granulocyte colony stimulating factor ( G CSF ) were elevated in the acute phase of KD . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study we investigated the expression of the following molecules : HLA class 1 ( ABC ) and class 2 ( HLA DR , HLA DP , HLA DQ ) , CD 29 , CD45RA , CD45RO , CD 56 , interleukin 1 ( IL 1 ) , IL 2 , IL 4 , interferon ( IFN gamma ) , tumor necrosis factor beta , and transforming growth factor beta 1 ( TGF beta 1 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our findings thus suggest that one anti inflammatory mechanism of GCS action may be through inhibition of the release of pro inflammatory cytokines IL 1alpha and beta , IL 2 , IFN gamma and TNF alpha , and up regulation of the anti inflammatory cytokine IL 10 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : The lymphokines interleukin ( IL ) 2 , IL 4 , interferon gamma ( IFN gamma ) and tumour necrosis factor beta ( TNF beta ) , as well as the monokines IL 1 , IL 6 and TNF alpha , were measured by immunoassays in sera and synovial fluid ( SF ) from patients with RA , REA and OA . ^^^ In contrast , cytokines were found in virtually all SF ; thus , the majority of SF from RA patients contained IFN gamma ( median level 17 pg / ml ) in addition to the monokines IL 6 ( 4700 pg / ml ) and TNF alpha ( 157 pg / ml ) . ^^^ IFN gamma and IL 6 ( but not TNF alpha ) were also frequently measured in SF from REA patients , whereas OA samples typically contained only IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These results suggest that in inflamed gingiva , pro inflammatory cytokines such as IL 1 beta and TNF alpha may induce bioactive intracellular IL 1 alpha production in human gingival fibroblasts and that this production can be differentially modulated by T cell derived cytokines such as IFN gamma or IL4 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pooled human immunoglobulin inhibits IL 4 but not IFN gamma or TNF alpha secretion following in vitro stimulation of mononuclear cells with Staphylococcal superantigen . ^^^ The authors found inhibition of secretion of interleukin 4 ( IL 4 ) ( P < 0 . 001 ) but not interferon gamma ( IFN gamma ) ( P=0 . 13 ) or tumour necrosis factor alpha ( TNF alpha ) ( P=0 . 66 ) by pooled immunoglobulin at concentrations ( 6 g / l ) which approximate the rise in serum immunoglobulin following in vivo IVIG therapy . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , IFN gamma , together with many other proinflammatory cytokines ( TNF alpha , IL 1beta , LPS , etc . ) , can stimulate the induction of inducible nitric oxide synthase ( iNOS ) and the generation of nitric oxide ( NO ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Peritoneal macrophages were subjected to stimulation with various concentrations of interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^ RESULTS : At all doses of IFN gamma and TNF alpha , DHA was found to be inhibitory to NO production . ^^^ CONCLUSIONS : DHA inhibits macrophage stimulated NO production in response to IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of cultures of highly purified term cytotrophoblasts with TNF alpha and IFN gamma had no effect on NO synthase ( NOS ) protein expression measured by immunoblot analysis : iNOS was not expressed and eNOS expression was unaffected . ^^^ We conclude that , although present in cultured villous cytotrophoblasts , ROI / RNI are not induced by TNF alpha and IFN gamma and do not mediate cytokine induced apoptosis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Isolated pancreatic islets were treated with IL 1beta , TNF alpha , and IFN gamma for 18 hours . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines such as interleukin 2 ( IL 2 ) , gamma interferon ( IFN gamma ) and alpha tumor necrosis factor ( TNF alpha ) are important mediators in immune responses against tumors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PBMC were examined for expression of CD 25 , CD 69 and CD 71 by immunofluorescence analysis and supernatants were assayed to quantify IFN gamma , TNF alpha and IL 4 by ELISA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) , interleukin 4 ( IL 4 ) , or IL 13 acted synergistically with CD 40 ligation to enhance TNF alpha production by ST cells . ^^^ IL 10 exerted inhibitory effects on both CD 40 ligation induced and CD 40 ligation plus IFN gamma induced TNF alpha production by ST cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) and tumour necrosis factor ( TNF ) induced nitric oxide as toxic effector molecule in chronic dextran sulphate sodium ( DSS ) induced colitis in mice . ^^^ It was therefore investigated whether inhibition of this synthase or the cytokines TNF and IFN gamma , inducers of nitric oxide synthase , had effects on chronic colitis in mice . ^^^ Neutralization of TNF and IFN gamma resulted in increased colon length ( 0 . 7 cm , P = 0 . 07 and 0 . 8 cm , P = 0 . 03 ) , improved histological score ( 19 % , P = 0 . 045 and 25 % , P = 0 . 013 ) , and reduced nitric oxide activity ( 31 % , P = 0 . 07 and 54 % , P = 0 . 004 ) compared with controls . ^^^ TNF and IFN gamma are involved in perpetuation of chronic DSS induced colitis , and induction of excessive nitric oxide activity could be their common effector mechanism . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , IL 12 treated elutriated MNC secreted no IFN gamma or tumor necrosis factor alpha but exhibited enhanced O 2 production compared to controls ( P = 0 . 013 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Sera from patients with SLE contained higher titers of IL 10 , and equal levels of IFN gamma and TNF alpha compared with those of normal controls . ^^^ Patients with progressive systemic sclerosis ( PSS ) showed lower titers of IL 10 and higher titers of IFN gamma and TNF alpha in their sera than those of healthy controls . ^^^ Seronegative rheumatoid arthritis ( snRA ) patients had a higher amount of IL 10 and TNF alpha , equivalent level of IFN gamma in their sera compared to those of controls . ^^^ Moreover , patients with Sjgren ' s syndrome ( SS ) showed higher titers of IL 10 and TNF alpha , and an equivalent level of IFN gamma in their sera compared to healthy volunteers . ^^^ CONCLUSION : Based on these findings , for the differential diagnosis of patients with systemic autoimmune diseases such as SLE , PSS , snRA and SS , it may be useful to measure the levels of cytokines such as IL 10 , IFN gamma , and TNF alpha in their sera . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
For example , the biological response to stress , anxiety states and anorexia nervosa includes the elevation of interleukin 1 beta ( IL 1 beta ) and tumor necrosis factor alpha ( TNF alpha ) , and downregulation of interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using C3H / He mice bearing MH 134 tumor cells , cytokine inductions of tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 beta ( IL 1 beta ) , interleukin 6 ( IL 6 ) and interferon gamma ( IFN gamma ) were determined in spleens by reverse transcriptase polymerase chain reaction ( RT PCR ) analysis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mice genetically deficient in the inducible NO synthase gene ( iNOS / ) were used to study the role played by NO during infection by Mycobacterium avium . iNOS / macrophages were equally able to restrict M . avium growth in vitro following stimulation by IFN gamma and TNF alpha as macrophages from wild type mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the lungs of CDC 1551 infected mice , small well organized granulomas with high levels of TNF alpha , IL 6 , IL 10 , IL 12 , and IFN gamma mRNA were apparent sooner than in lungs of mice infected with the other strains . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) , but not lipopolysaccharide ( LPS ) , had a augmentative effect on IFN gamma induced EC apoptosis ( p < . 05 ) , while both of them alone failed to induce EC apoptosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Proinflammatory cytokines , such as TNF alpha and IFN gamma , showed a moderate focal expression on the dermal perivascular sites ; IL 8 was found to have a particularly intense staining on all the epidermal cell layers and at perivascular and vascular sites . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The production of interferon ( IFN ) gamma , tumour necrosis factor ( TNF ) alpha and interleukin ( IL ) 10 was investigated in culture supernatants , and the cellular sources of IFN gamma and TNF alpha were identified . ^^^ Cells from individuals with a history of cutaneous leishmaniasis produced significantly higher levels of IFN gamma and TNF alpha than cells from individuals without a history of the disease . ^^^ Flow cytometric analysis revealed high numbers of CD3+ cells producing IFN gamma and TNF alpha , and only a few CD3+ cells containing IL 10 , in the PBMC cultures from the individuals with a history of cutaneous leishmaniasis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the molecular mechanism for the synergistic induction of inducible nitric oxide synthase transcription by TNF alpha and IFN gamma . ^^^ Since TNF alpha and IFN gamma stimulate cells in part by activating NF kappaB and IRF 1 , we hypothesized that these two transcription factors interact with each other . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Differential regulation of IFN gamma , TNF alpha , and IL 10 production by CD 4 ( + ) alphabetaTCR+ T cells and vdelta 2 ( + ) gammadelta T cells in response to monocytes infected with Mycobacterium tuberculosis H37Ra . ^^^ CD 4 ( + ) and gammadelta T cells were compared for their ability to regulate IFN gamma , TNF alpha , and IL 10 production , cytokines with significant roles in the immune response to M . tuberculosis . ^^^ Thus CD 4 ( + ) and gammadelta T cells had similar roles in differential regulation of IFN gamma , TNF alpha , and IL 10 secretion in response to M . tuberculosis infected monocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Initiation of apoptosis can be achieved by the combinations of IFN gamma / TNF alpha , IFN gamma / CD95 , IL 1alpha / IFN gamma , and IL 1alpha / IFN gamma / TNF alpha to varying extents . ^^^ Pretreatment with IL 13 protects against IL 1alpha / IFN gamma / TNF alpha and IFN gamma / TNF alpha as well as IFN gamma / CD95 induced ( but not IL 1alpha / IFN gamma induced ) cell death . ^^^ In addition , IFN gamma / TNF alpha and IL 1alpha / IFN gamma / TNF alpha stimulate activation of caspase 8 and caspase 3 , which IL 13 pretreatment was able to partially inhibit and delay . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The in vivo treatment with anti CD 1 mAb reduced TGF beta 2 levels and concomitantly increased secretion of the proinflammatory cytokine TNF , the Th 1 cell promoting cytokine IL 12 , and the Th 1 cell cytokine IFN gamma at the onset of listerial infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 5 , TNF alpha , and IFN gamma production were also inhibited , but to a lesser degree than IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cells stimulated with the proinflammatory cytokines IL 1beta , TNF alpha , IFN gamma , and IL 15 , respectively , increased significantly their intracellular as well as cell surface bound FasL expression in a time and dose dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The ability to control fungus growth in lungs is associated with vigorous TNF alpha , but not IFN gamma , production by bronchoalveolar lavage cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Skin derived IFN gamma / inflammatory cells , but not cells from anti IL 12 treated animals , secreted substantial amounts of TNF alpha , suggesting that the inflammatory effect of IFN gamma / T cells may be partly exerted by TNF alpha and that the therapeutic effect of anti IL 12 may depend on its ability to down regulate both TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
They specifically recognize different types of glycolipids , such as gangliosides , sulfatide and galactosylceramide and release IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The role of cell cell contact in the induction of apoptosis was evaluated in a transwell system in the presence or absence of neutralizing antibodies against IFN gamma and tumor necrosis factor ( TNF ) alpha . ^^^ This suppressive effect was not mediated by the secretion of IFN gamma or TNF alpha molecules . ^^^ The HFRPE induced apoptosis was not mediated through the FasL / Fas pathway or through the secretion of the apoptosis inducing cytokines IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
On day 21 , the splenocytes and tumor tissue infiltrating lymphocytes were analyzed by FACScan and RT PCR for the mRNA of the genes that encode tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , TGF beta , interleukin ( IL ) 4 , IL 10 and IL 6 . ^^^ In both spleen and tumor tissue after low dose TBI , mRNA expression of the genes that encode IFN gamma and TNF alpha increased , while that of the Tgfb gene decreased . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The HIV negative Ethiopians had exceedingly high blood levels of eosinophils , immunoglobulin E ( IgE ) , and p75s tumor necrosis factor receptors ( p75sTNFR ) ; secretion of interleukin 4 ( IL 4 ) and IL 10 by peripheral blood mononuclear cells ( PBMC ) ; proportion of human leukocyte antigen ( HLA ) DR+ cells within CD3+ , CD4+ , and CD8+ T cell subsets ; and proportion of CD45RO+ CD4+ cells ; while having significantly lower secretion of interferon gamma ( IFN gamma ) by PBMC and percentage of CD45RA+ CD4+ and CD28+ CD8+ cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma but not alpha or beta potently inhibited tumor necrosis factor ( TNF ) alpha induced eotaxin generation by dermal fibroblasts . ^^^ IFN gamma did not inhibit , but rather augmented the TNF alpha induced accumulation of mRNA in the early phase ( approximately 6 h ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokines IL 2 , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) , and the early activation marker CD 69 , were assessed by four colour flow cytometry of whole blood cultures taken before and after IVIG infusion . ^^^ This increase in TNF alpha and IL 2 , combined with unchanged IFN gamma expression , is evidence against the putative ' anti inflammatory ' role of IVIG , and may explain the failure of resolution of granulomata in CVID patients treated with IVIG alone . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inhibition of CFU E colony formation in uremic patients with inflammatory disease : role of IFN gamma and TNF alpha . ^^^ Treatment of parallel cultures with a combination of antibodies to interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) almost completely restored the response to EPO . ^^^ Additionally , bone marrow from healthy controls incubated with uremic sera showed an increased production of interleukin 1 alpha ( IL 1 alpha ) and IFN gamma , and TNF alpha was present in uremic sera . ^^^ These soluble factors stimulate the production of IFN gamma and TNF alpha , which directly inhibit erythropoiesis at a local level in the bone marrow . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It has been demonstrated that endogenous cytokines including gamma interferon ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) , and interleukin 6 ( IL 6 ) play protective roles but that IL 4 and IL 10 play detrimental roles in nonlethal Listeria monocytogenes infection in mice . ^^^ The maximum production of IFN gamma was observed before the peaks of TNF alpha and IL 6 , and IFN gamma almost disappeared from the bloodstreams and organs just before the mice died . ^^^ The administration of monoclonal antibodies ( mAbs ) against TNF alpha , IFN gamma , IL 6 , IL 4 or IL 10 failed to rescue mice from lethal L . monocytogenes infection , whereas anti TNF alpha mAb and anti IFN gamma mAb prevented mice from lethality by high dose endotoxin shock . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We report here that perforin deficient mice exhibit severe immunopathology in multiple organs that is characterized by infiltration of anti LCMV CTL that secrete large amounts of gamma interferon ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^ This is due to the selective reduction of NP specific CTL responses and their inflammatory cytokine ( IFN gamma and TNF alpha ) secretion and to a lack of pathogenetically relevant compensatory responses to other viral proteins . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The resulting cell supernatants were analyzed for the proinflammatory cytokines ( TNF alpha , IL 6 ) , the chemotactic active interleukin 8 as well as cytokines from T helper type 1 ( IL 2 , IFN gamma , IL 12 ) and type 2 ( IL 4 , IL 10 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After 4 to 24 hours of incubation , interleukin 2 ( IL 2 ) , IL 4 , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) production was measured by three color flow cytometry . ^^^ The results show that cord blood lymphocytes ( CBL ) produce less IL 2 , IL 4 , IFN gamma , and TNF alpha than adult peripheral blood lymphocytes ( ABL ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Lymphoproliferative responses with phytohemagglutinin ( PHA ) and the capacity of whole blood to produce cytokines , such as interleukin 1 ( IL 1 ) , IL 6 , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and granulocyte colony stimulating factor ( G CSF ) , were longitudinally measured before and after weight gain , that is , at admission and at less than 60 , 65 , and 75 % of standard body weight ( SBW ) , in 17 patients with restricting type anorexia nervosa and in 17 control subjects . ^^^ RESULTS : Cytokine production of IL 1 , IL 6 , and TNF alpha per monocyte in the anorexic patients recovered only with the start of refeeding , whereas IFN gamma production per lymphocyte was similar to that in control subjects and did not change during weight restoration . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study , we have investigated the effects of interferons alpha ( IFN alpha ) and gamma ( IFN gamma ) , interleukin 10 ( IL 10 ) and 13 ( IL 13 ) , transforming growth factor beta 1 ( TGF beta 1 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and tumor necrosis factor alpha ( TNF alpha ) on cell proliferation and induction of transcription factors AP 1 and NF kappaB in UM EC 3 human endometrial adenocarcinoma cells and UT OC 5 ovarian carcinoma cells in vitro . ^^^ In UM EC 3 cell line DNA synthesis was inhibited by GM CSF , IL 10 , IL 13 , TGF beta 1 , IFN alpha , and IFN gamma after 48 and 72 h in culture , whereas TNF alpha had no significant effect on cell proliferation in any of the experiments . ^^^ The inhibition of DNA synthesis was similarly observed in UT OC 5 ovarian carcinoma cells by IL 10 , TNF alpha , and IFN gamma after 48 and 72 h , whereas IFN alpha had no statistically significant effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Protein A ( PA ) of Staphylococcus aureus is known to elicit several cytokines such as IFN gamma , TNF alpha and IL 1 . ^^^ Cytokines , such as IFN gamma , IL 2 , IL 4 , IL 6 , IL 10 , TNF alpha , IL1alpha and IL1beta were measured in serum by ELISA . ^^^ Our results show that 1 microg dose of PA stimulates the production of IFN gamma ( 115 + / 5 pg / ml ) , TNF alpha ( 250 + / 8 pg / ml ) and IL1alpha ( 100 + / 5 pg / ml ) as compared to control levels of , 22 + / 2 , 20 + / 2 and 35 + / 3 pg / ml respectively whereas IL 2 and IL1beta secretion were less ( beyond the lower detection limit of the kit and 25 + / 1 pg / ml , respectively ) as compared to control ( 28 + / 2 and 52 + / 4 pg / ml , respectively ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Proinflammatory ( TNF alpha and IL 12 ) and type 1 cytokines ( IFN gamma and IL 2 ) significantly upregulated CCR 5 ( wt allele ) mRNA expression in CCR 5 homozygous wild type ( wt / wt ) and heterozygous individuals ( wt / del ) ( P < 0 . 02 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To investigate a new kind of anti tumor immunological cells and improve surgical results of hepatocellular carcinoma by anti recurrence application , we activated T cells isolated from tumor infiltrating lymphocytes by double stimulating signals : the one was autologus HCC cells which were treated with IFN gamma and TNF alpha for enhancing expression of MHC class 1 and presented tumor antigen , and the other was costimulation signals which was from ICAM 1 and B 7 molecules expressed on treated HCC cells as well as CD 28 mAbs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The T helper 1 type cytokine interferon gamma ( IFN gamma ) induced TNF transcripts , but not TNF secretion , and suppressed LPS induced IL 10 mRNA and secretion by microglia . ^^^ Opposite to IFN gamma , the lipid mediator prostaglandin E 2 ( PGE 2 ) enhanced the LPS induced production of IL 10 and inhibited that of TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Peritoneal fluids were collected from 30 subjects with adhesions ( n = 22 ) or with normal pelvic anatomy ( n = 8 ) , and the level of interleukin 1 beta ( IL 1 beta ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 10 ( IL 10 ) , interferon gamma ( IFN gamma ) , transforming growth factor beta 1 ( TGF beta 1 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , prostaglandin E 2 ( PGE 2 ) , leukotriene B 4 ( LTB 4 ) , and thromboxane B 2 ( TXB 2 ) were determined by enzyme linked immunosorbent assay ( ELISA ) and radioreceptor assay . ^^^ RESULTS : The peritoneal fluid content of these factors varied considerably , with low levels of IL 1 beta , TNF alpha , IL 10 , IFN gamma , and GM CSF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
More TNF alpha , IL 2 , and IFN gamma were produced by unstimulated and stimulated cultured peripheral blood mononuclear cells from preeclampsia patients than by those from normal subjects . ^^^ TNF alpha / IL 4 , IL 2 / IL 4 , and IFN gamma / IL 4 ratios were higher in preeclamptic patients than in the normal group . ^^^ Significant positive correlations were observed between mean blood pressure and concentrations of the Th 1 type cytokines IL 2 , IFN gamma , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The tested compounds segregated into two distinct classes : one class of compounds , shown to be potent phosphodiesterase 4 inhibitors , inhibited TNF alpha production , increased IL 10 production by LPS induced PBMC , and had little effect on T cell activation ; the other class of compounds , similar to thalidomide , were not phosphodiesterase 4 inhibitors and markedly stimulated T cell proliferation and IL 2 and IFN gamma production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neither IFN gamma plus TNF alpha nor CD 40 stimulation significantly induced B 7 1 or up regulated B 7 2 on human myeloma cell line or primary myeloma cells from six of seven patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To elucidate regulatory importance , mAab to interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) dose dependently inhibited IFN gamma induced MHC expression by peritoneal macrophages and TNF alpha induced thymocyte proliferation , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , the activating effect of exogenous Nef on PBMC proliferation was associated with an increase of IFN gamma , TNF alpha , and IL 6 production , while , surprisingly , IL 2 production was not affected by Nef . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The ceramide effect on cell viability mimicked the effect of the cytokines TNF alpha , IL 1beta , and IFN gamma , reported stimulators of sphingomyelin hydrolysis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Proportionately , more allografts than autografts contained transcripts for IL 2 and TNF alpha , and IFN gamma was detected in three of four allografts . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We studied the serum levels of IL 2 , IFN gamma and TNF in different clinical forms of Chagas ' disease and in patients clinically compensated and decompensated . ^^^ Serum TNF was undetectable and IFN gamma levels did not differ between clinical forms and severities of Chagas ' disease . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma and TNF alpha by tonsillar mononuclear cells at a significant level . ^^^ These data suggest that IL 6 , IFN gamma and TNF alpha may play an important role in immunological reactions in the tonsils and that a hyper immune response to some of alpha streptococcal antigens through increased production of such cytokines by tonsillar mononuclear cells , to which T cells mainly contribute , may play a key role in the pathogenesis of PPP . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A search of the mechanism ( s ) of such differential action of PA at these two doses revealed that the lower dose of PA upregulated the production of interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) to the extent which has already been reported by our laboratory to be beneficial to the host . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interestingly , treatment with COX 2 specific inhibitors such as NS 398 or Celecoxib severely diminished early and late events of T cell activation , including CD 25 and CD 71 cell surface expression , IL 2 , TNF alpha , and IFN gamma production and cell proliferation , but not the expression of CD 69 , an immediate early gene . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The combination of tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) induces apoptosis of primary placental trophoblasts in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cutting edge : combined treatment of TNF alpha and IFN gamma causes redistribution of junctional adhesion molecule in human endothelial cells . ^^^ Proinflammatory cytokines such as TNF alpha and IFN gamma induce cell adhesion molecules in endothelial cells and promote transmigration of leukocytes across endothelial cells . ^^^ The combined treatment of TNF alpha plus IFN gamma caused a disappearance of JAM from intercellular junctions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We show that TRAIL is inducible by IFN gamma , by TNF alpha , and by infection with human CMV , and has potent antiviral activity in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Alloantibody mediated class 1 signal transduction in endothelial cells and smooth muscle cells : enhancement by IFN gamma and TNF alpha . ^^^ Treatment of cells with IFN gamma and TNF alpha up regulated MHC class 1 expression and potentiated anti HLA Ab induced fibroblast growth factor receptor expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
For these cell types , IFN gamma was a potent inducer of CXCL 11 transcription , which was synergistically enhanced by TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine secretion was analyzed in culture medium , and we detected significant levels of IFN gamma , TNF alpha , and IL 10 and no IL 4 ( except in one case ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Vgamma9 / Vdelta2 cells from the CSF of TBM children responded to different synthetic and natural ( mycobacterial ) phosphoantigens and produced discrete amounts of IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation of PBMCs by SEB or TSST 1 was effectively blocked by pentoxifylline ( 10 mM ) , as evidenced by the inhibition of TNF alpha , interleukin 1beta ( IL 1beta ) , gamma interferon ( IFN gamma ) , and T cell proliferation . ^^^ The levels of TNF alpha , IL 1alpha , and IFN gamma in serum after an SEB or TSST 1 injection were significantly lower in mice given pentoxifylline ( 5 . 5 mg / animal ) versus control mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human beta cell preparations were cultured for 48 or 72 hours with or without IL 1beta , TNF alpha , or IFN gamma , alone or in combination . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Venous blood was sampled for biochemical and hematological analysis and for IL 1alpha , IL 6 , IL 10 , TNF alpha , IFN gamma and GM CSF ELISAs at the time of hospital admission , 6 h ( moderate and severe cases ) , and 12 , 18 , 36 and 72 h ( severe case ) later . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The list of cytokines analysed includes 21 molecules : interleukins ( IL ) 1 = > 8 , IL 10 = > 13 , IL 15 , granulocyte monocyte colony stimulating factor ( GM CSF ) , interferon gamma ( IFN gamma ) , oncostatin M ( OSM ) , regulated upon activation normal T cell expressed and presumably secreted ( RANTES ) , transforming growth factor beta 1 ( TGF beta 1 ) , tumor necrosis factor alpha ( TNF alpha ) and vascular endothelial growth factor ( VEGF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To investigate this hypothesis the in situ release by BAL cells of the following cytokines was measured and correlated with the chest 10 ray findings of 43 patients with pulmonary tuberculosis : interleukin ( IL ) 8 , macrophage inflammatory protein 1alpha ( MIP 1alpha ) , IL 6 , tumor necrosis factor alpha ( TNF alpha ) , transforming growth factor beta ( TGF beta ) , interferon gamma ( IFN gamma ) , IL 2 , IL 4 and IL 5 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , based on the utilization of neutralizing antibody to TNF alpha or the inhibition of NO production , TNF alpha and NO were proven to be involved as mediators during the activation of tumoricidal macrophages by protein A in combination with IFN gamma . ^^^ We also demonstrated that supernatants from macrophages treated with protein A plus IFN gamma contained both TNF alpha and NO at markedly increased levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Enhancing effect of tumor necrosis factor ( TNF ) alpha , but not IFN gamma , on the tumor specific cytotoxicity of gammadeltaT cells from glioblastoma patients . ^^^ We then examined the effect of TNF alpha and IFN gamma on these tumor specific gammadeltaT cells for possible clinical use in cancer patients . ^^^ TNF alpha alone , at concentrations of 0 . 01 1 . 0 microg / ml , caused increased gammadeltaT cell cytotoxicity against autologous glioblastoma cells , whereas IFN gamma alone had no effect . ^^^ TNF alpha , but not IFN gamma , marginally inhibited the proliferative response of gammadeltaT cells ; a similar result was seen when the cytokines were combined . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) have been implicated in IBD . ^^^ METHODS : T 84 cells were cultured with IFN gamma and TNF alpha and changes in transepithelial resistance ( TER ) , fluorescein isothiocyanate ( FITC ) dextran flux , short circuit current ( 1 ( sc ) ) , cystic fibrosis transmembrane conductance regulator ( CFTR ) protein levels , cell morphology , TNF receptor gene expression , and apoptosis were assayed . ^^^ When added with IFN gamma , TNF alpha accelerated IFN gamma dependent changes . ^^^ Relative to controls , significant changes occurred after one day of culture with IFN gamma plus TNF alpha : TER was decreased to 27 ( 3 . 5 ) % , FITC dextran flux was increased by 185 ( 45 ) fold , and cAMP and Ca dependent 1 ( sc ) were decreased to 66 ( 12 ) % and 35 ( 6 . 8 ) % , respectively . ^^^ TNF alpha also enhanced IFN gamma dependent changes in cell morphology but did not induce cell death . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Several Th 1 type ( IL 2 , IL 12 , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) ) and Th 2 type ( IL 4 , IL 5 , IL 10 , IL 13 ) cytokines were secreted by UCBC and PBMC in response to OvAg , bacterial SL O and PHA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokines , interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and interleukin 2 ( IL 2 ) are important endogenous proinflammatory proteins and have been linked to disease activity in multiple sclerosis . ^^^ In this study , we use flow cytometric methodology to compare the secretion of IFN gamma , IL 2 , and TNF alpha from peripheral blood derived T cells of multiple sclerosis patients to the secretion in healthy controls . ^^^ The percentages of IFN gamma , IL 2 , and TNF alpha secreting cells are not significantly different between multiple sclerosis patients and controls . ^^^ However , the TNF alpha secreting CD 3 cell percentage is correlated with the IFN gamma and IL 2 secreting CD 3 cell percentages in multiple sclerosis patients . ^^^ In the controls , only the TNF alpha secreting CD 3 cell percentage is correlated with IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
COACTS were induced to secrete high levels of Th 1 type cytokines ( IFN gamma and TNF alpha ) after a 24 h restimulation with fresh 3 / 28 beads and displayed nonmajor histocompatibility complex restricted lytic activity against a variety of human tumor cell lines in standard 51Cr release assays . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Astrocyte derived monocyte chemoattractant protein 1 ( MCP 1 ) , RANTES , macrophage inflammatory protein 1alpha ( MIP 1alpha ) , and MIP 1beta mRNA were easily induced by lipopolysaccharide and / or the proinflammatory cytokines ( IFN gamma and / or TNF alpha ) , respectively . ^^^ Addition of both IFN gamma and TNF alpha together did not lead to an additive effect but resulted in the inhibition of MCP 1 and MIP 1beta mRNA expression , indicating that interaction between chemokines and cytokines may play a key role in regulating the local immune response of resident and infiltrating cells at the site of lesion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Induction of transcription factor interferon regulatory factor 1 by interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) in FRTL 5 cells . ^^^ While it is well known that interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) play a role in the regulation of thyroid growth and differentiated functions , the cellular and molecular mechanisms involved in mediating the effects of IFN gamma and TNF alpha on thyroid function are unknown . ^^^ In the present study , we used FRTL 5 rat thyroid cells to examine the effects of IFN gamma and TNF alpha on gene expression of transcription factor interferon regulatory factor 1 ( IRF 1 ) , which is involved in mediating the effects of these cytokines in a number of cell types . ^^^ Incubation of FRTL 5 cells with IFN gamma or TNF alpha resulted in a dose and time dependent increase in IRF 1 mRNA levels . ^^^ We have shown that TNF alpha and IFN gamma act synergistically to block the TSH induced increase in type 1 5 ' deiodinase ( 5 ' D 1 ) activity and 5 ' D 1 gene expression in FRTL 5 rat thyroid cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There was no difference between KAs and SCCs in expression of lymphotoxin alpha , IL 2 , interferon gamma ( IFN gamma ) , IL 13 , transforming growth factor beta ( TGF beta ) , or the pro inflammatory cytokines IL 8 or tumour necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro effects of IFN gamma , TNF alpha , TGF beta 1 , IL 4 and IL 10 on DC were examined . ^^^ IFN gamma enhanced proliferation and NO production by DC , while TNF alpha and IL 4 induced only slight DC proliferation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Activation of microglia by ligation of CD 40 in the presence of IFN gamma results in cultured cortical neuronal injury , which is markedly attenuated by blockade of the CD 40 pathway or neutralization of TNF alpha . ^^^ Finally , we find significant levels of IFN gamma and TNF alpha in the medium of co cultured activated CD4+ T cells and microglial cells , showing that microglia can supply the CD 40 receptor to activated CD4+ T cells and suggesting that this cellular interaction is a key event in MS pathophysiology . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Production of nitrites ( index of NO synthesis ) , tumor necrosis factor alpha ( TNFalpha ) , interleukin 1beta ( IL 1beta ) , and interferon gamma ( IFN gamma ) by unstimulated or stimulated peritoneal leukocytes originating from NAG based or glucose based fluid was measured . ^^^ Stimulated peritoneal leukocytes from NAG based dialysate produced more cytokines than did cells from glucose based dialysate : TNFalpha , 135 . 2+ / 37 . 0 pg versus 70 . 2+ / 21 . 8 pg per 10 ( 6 ) cells respectively , p < 0 . 01 ; IL 1beta , 143 . 2+ / 60 . 9 pg versus 99 . 1+ / 22 . 4 pg per 10 ( 6 ) cells respectively , p < 0 . 05 ; IFN gamma , 16 . 2+ / 12 . 5 pg versus 6 . 0+ / 1 . 8 pg per 10 ( 6 ) cells respectively , p < 0 . 01 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
During peritonitis , we found a higher overall dialysate concentration of both tumor necrosis factor ( TNFalpha : +53 % , p < 0 . 05 ) and of interferon gamma ( IFN gamma : +303 % , p < 0 . 02 ) , in the Bic Lac group than in the Lac group . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CD8+ cells from OA / OA mice demonstrated intracellular staining for IL 2 ( 26 % ) , TNF alpha ( 55 % ) , and IFN gamma ( 37 % ) , but not IL 4 or IL 5 , after polyclonal activation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The iNOS transcriptional activity in RSV infected cells was significantly enhanced by an exogenous cytokine mixture ( IL 1beta , TNF alpha , and IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , TSH / forskolin decrease ICAM 1 RNA levels that are maximally induced by two cytokines : 100 ng / mL tumor necrosis factor alpha ( TNF alpha ) or 100 U / ml interferon gamma ( IFN gamma ) . ^^^ The effect of TSH / forskolin , as well as TNF alpha and IFN gamma , on ICAM 1 RNA levels is transcriptional . ^^^ Thus , we cloned a 1 . 8 kb fragment of the 5 ' flanking region of the rat ICAM 1 gene , upstream of the translational start site , and showed that TNF alpha or IFN gamma caused a 3 . 5 and greater than 12 fold increase respectively , in its promoter activity , when linked to a luciferase reporter gene and stably transfected into FRTL 5 cells . ^^^ TSH or forskolin , in contrast , halved the activity of the full length chimera within 24 hours and significantly suppressed the TNF alpha and IFN gamma induced increase ( > 50 % ; p < 0 . 02 ) . ^^^ We thus show that TSH / cAMP can downregulate ICAM 1 gene expression and inhibit the activity of cytokines ( TNF alpha and IFN gamma ) to increase ICAM 1 gene expression in FRTL 5 thyroid cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Microdissection of biopsy material from labial minor salivary glands followed by RT PCR demonstrates extensive production of cytokines IL 2 , 1L 6 , IL 10 , TNF alpha , TGF beta , and IFN gamma in both normal glands and in glands affected by Sjgren ' s syndrome . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increased expression of laminin and various cytokines , including interferon y ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) has been demonstrated in minor salivary glands from patients with Sjgren ' s syndrome . ^^^ Treatment of HSG cells for 3 days with IFN gamma ( 1000 U / ml ) and TNF alpha ( 20 U / ml ) resulted in no significant changes in cell proliferation or laminin protein and / or mRNA species compared to cells treated with IFN gamma alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mice with SGVHD had increased levels of message for IL 12p40 , IFN gamma , and TNF alpha in the target organs of SGVHD as compared with transplant controls and asymptomatic cyclosporine A treated mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cultivation of islets with IL 18 affected NO production or mitochondrial activity and did not protect from the toxicity mediated by IL 1beta , TNF alpha , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The detection of elevated levels of IL 6 , TNF alpha , and IFN gamma in rat cerebrospinal fluid 6 h after intracisternal Nef injection hint at the additional involvement of indirect mechanisms in Nef induced leukocyte migration into rat CNS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here , we show that induction of hsp 70 expression induces an infiltrate of T cells , macrophages , and predominantly dendritic cells ( DCs ) into the tumors as well as an intratumoral profile of Th 1 cytokine expression ( IFN gamma , TNF alpha , and IL 12 ) and enhances immunogenicity via a T cell mediated mechanism . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha suppresses IFN gamma induced MHC class 2 expression in HT 1080 cells by destabilizing class 2 trans activator mRNA . ^^^ TNF alpha is commonly coexpressed with IFN gamma during immune mediated inflammatory responses and modulates IFN gamma stimulated MHC class 2 expression . ^^^ We show here that TNF alpha suppresses IFN gamma induced CIITA mRNA accumulation , resulting in decreased MHC class 2 expression in human fibrosarcoma HT 1080 cells . ^^^ CIITA mRNA , regulated by either IFN gamma or tetracycline , was destabilized in the presence of TNF alpha , suggesting that TNF alpha utilizes a distinct mechanism to suppress MHC class 2 expression in HT 1080 cells . ^^^ Consistent with this interpretation , TNF alpha blocked IFN gamma induced CIITA and MHC class 2 expression in mutant cells that are unresponsive to TGF beta or IFN beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 13 as well as IL 4 up regulated eotaxin 3 mRNA in HUVEC , whereas neither TNF alpha , IL 1beta , IFN gamma , nor TNF alpha plus IFN gamma did . ^^^ The expression profile of eotaxin 3 is different from those of eotaxin , RANTES , and monocyte chemoattractant protein 4 , which are potent eosinophil selective chemoattractants and are induced by either TNF alpha or TNF alpha plus IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have investigated the role of a number of cytokines ( IL 1 beta , TNF alpha , IL 4 , IL 6 , IL 10 , IL 12 , TGF beta 1 , IFN gamma ) in regulating B7 . 1 and Fas expression . ^^^ IFN gamma ( 500 U / mL ) and TNF alpha ( 200 ng / mL ) were unable to induce de novo expression of B7 . 1 or Fas on cultured TFC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of Balb / c male mice for two weeks resulted in the increase of IL 4 , and the decrease of TNF alpha , IFN gamma , and IL 2 release from stimulated splenocytes , suggesting that UK 114 modulates the Th1 / Th2 cytokine profile toward Th 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , we will discuss the role of the cytokines TNF alpha , IFN gamma , and IL 6 and its effects on pancreatic cancer cell proliferation in vitro and in vivo . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : ( 1 ) The production of mitogen stimulated IFN gamma and TNF alpha as well as the production of env peptide stimulated IFN gamma , TNF alpha , and IL 10 are increased in African HIV infection ; ( 2 ) the expression of activation and Th 2 associated markers is augmented in African HIV infection as is the memory / naive ratio ; ( 3 ) mitogen stimulated IFN gamma and IL 10 production , as well as the expression of activation and Th 2 associated markers and the memory / naive ratio , are augmented in African compared with Italian controls ; and ( 4 ) plasma viraemia is reduced in African compared with Italian HIV infected individuals . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha , nitric oxide and IFN gamma are all critical for development of necrosis in the small intestine and early mortality in genetically susceptible mice infected perorally with Toxoplasma gondii . ^^^ We examined the role of tumour necrosis factor ( TNF ) alpha and nitric oxide ( NO ) , in addition to IFN gamma . ^^^ At 7 days after infection , a marked increase in CD4+ T cells was observed in lamina propria mononuclear cells ( LPC ) of the small intestine as compared with normal mice , and significantly greater amounts of mRNA for IFN gamma , TNF alpha , and inducible NO synthase ( iNOS ) were detected in LPC of the small intestine of infected than uninfected animals . ^^^ Treatment with anti TNF alpha mAb did not affect the expression of IFN gamma in the LPC but inhibited expression of iNOS in the infected mice , indicating the role of TNF alpha in the induction of iNOS . ^^^ These results suggest that NO induced by a combination of IFN gamma and TNF alpha through activation of iNOS is a critical mediator of intestinal pathology and contributes to early mortality in genetically susceptible mice . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cryostat sections of cornea were taken at different times after challenge and examined for infiltrating cells containing IL 2 , IL 4 , IFN gamma , IL 6 , or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The level of IFN gamma or TNF alpha was found to diminish in T . gondii treated mice as the infection progressed to the late stage . ^^^ This declined response of IFN gamma or TNF alpha was associated with marked increase in the expression of IL 10 , a counterregulatory cytokine . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We show here that the reduction of LPL activity in J774 . 2 macrophages observed in the presence of interleukin ( IL 1 ) and IL 11 was sensitive to herbimycin A , with the effect of LPS , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) on LPL activity being sensitive to both herbimycin A and wortmannin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The role of Bcl 2 expression in EGF inhibition of TNF alpha / IFN gamma induced villous trophoblast apoptosis . ^^^ The inflammatory cytokines tumour necrosis factor alpha ( TNF alpha ) and immune interferon gamma ( IFN gamma ) stimulate villous cytotrophoblast apoptosis while epidermal growth factor ( EGF ) protects . ^^^ We hypothesize that TNF alpha , IFN gamma and EGF regulate apoptosis in part by modulating cellular expression levels of the anti death gene bcl 2 . ^^^ Culture with TNF alpha / IFN gamma and EGF did not alter expression of either Bcl 2 protein or of the pro apoptotic Bcl 2 family member Bak . ^^^ Double label flow cytometric analysis that measured apoptosis and Bcl 2 content simultaneously showed that cells expressing low levels of Bcl 2 underwent TNF alpha / IFN gamma induced apoptosis at a higher frequency than cells expressing lower levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Macrophage derived cytokines , such as IL 1 , TNF alpha , and IFN gamma , activate endothelial cells , upregulating the expression of adhesion molecules such as ICAM 1 and VCAM 1 , which permit egression of leukocytes from the vasculature to the airway mucosa . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The mRNA for the proinflammatory cytokines interleukin ( IL ) 1alpha , IL 1beta , IL 6 , macrophage inflammatory protein 2 ( MIP 2 ) , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) were measured by semiquantitative reverse transcription polymerase chain reaction using beta actin as an internal standard . ^^^ During exposure to volatile anesthetics , gene expression for IL 1beta , MIP 2 , IFN gamma , and TNF alpha all increased significantly compared with mechanical ventilation alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The combination of anti IL 12 and anti TNF treatment was found to reduce collagen type 2 ( CII ) specific lymph node cell IFN gamma production and proliferation , as well as decrease the anti CII IgG2a : IgG 1 ratio more effectively than either treatment alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , no differences were found in the serum IL 12 , IFN gamma , TNF alpha and nitric oxide ( NO ) production in the culture of macrophages between the HET and PBS treated mice on day 2 after MCMV infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Control CBA 10 DBA / 2 pregnant mice , or from mice injected with TNF alpha + IFN gamma on day 7 . 5 of gestation , were removed on day 8 . 5 , fixed , sectioned , and subject to in situ hybridization for Fgl 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The inhibition of proliferation correlated with interference with the IL 2 / IL 2 receptor system , while the effects of rolipram on several T helper 1 ( TNF alpha , TNF beta , IFN gamma ) and T helper 2 like cytokines ( IL 4 , IL 13 ) as well as IL 10 revealed an interesting drug profile , with preferential inhibition of TNF beta , TNF alpha and IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunohistochemical results show a higher degree of expression of iNOS , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) in brains of rats with acute monophasic EAE relative to the control animals . ^^^ Administration of lovastatin inhibited the expression of iNOS , TNF alpha and IFN gamma in the CNS of EAE rats and improved the clinical signs of EAE suggesting that this compound may have therapeutic potential in the treatment of neuroinflammatory diseases like MS . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Antibody treatment reduced IFN gamma concentrations in a dose related manner , but had no obvious effects on levels of other cytokines in this small study , although unbound TNF alpha was undetectable after Fab treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Several antiviral acyclic nucleotide analogues activate expression of genes for cytokines , such as TNF alpha , IL 10 in macrophages and IFN gamma in splenocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increases in calcium also occurred with interleukin 1beta ( IL 1beta ) and tumor necrosis factor alpha ( TNF alpha ) , but not to the extent seen with IFN gamma . ^^^ Increased cyclic guanosine monophosphate ( cGMP ) was involved in the results described with short term ( 2 hr ) TNF alpha and long term ( 18 hr ) IFN gamma treatments . ^^^ Our findings suggest that TNF alpha acts via a cGMP dependent pathway , whereas the actions of IFN gamma involve adenylate cyclase , and possibly a NO forming mechanism and cGMP pathway as well . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the acute phase ( days 13 16 ) all three organs became involved : ( 1 ) in the SM , significant elevations were limited to IL 1beta and IL 6 , which , notably , correlated positively with the degree of arthritis ; ( 2 ) in the popl LN , IFN gamma , IL 1beta , IL 6 , and IL 10 ( but not IL 5 ) were still elevated , while IL 2 rose significantly ; ( 3 ) in the spleen , TNF alpha peaked simultaneously with the arthritis score ( day 16 ) and dramatically dropped thereafter . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The addition of interferon gamma ( IFN gamma ) to the culture medium did not alter TNF alpha and NO secretion by the EP 3 activated macrophages , however , IL 8 secretion was increased when a low concentration of IFN gamma ( 0 . 2 U / ml ) was added , but was reduced in the presence of a high concentration of IFN gamma ( 2000 U / ml ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Whole blood ( diluted 1 : 10 ) and mononuclear cell ( MNC ) cultures were stimulated for 24 h with either concanavalin A ( Con A ) or bacterial lipopolysaccharide ( LPS ) and the concentrations of IL 1alpha , IL 1beta , IL 2 , TNF alpha , IL 10 and IFN gamma in the culture medium measured . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , the expression of IFN gamma mRNA , as well as tumor necrosis factor alpha and interleukin 6 mRNA , was not affected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
An encephalitogenic DA T cell line expressed mRNA for the Th 1 cytokines IFN gamma and TNF alpha , as well as IL 10 , and secreted these cytokines . ^^^ Although this line did not express TNF alpha or IL 10 mRNA , IFN gamma mRNA was detected , suggesting posttranscriptional regulation of IFN gamma expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we show that a combination of the same inflammatory cytokines increased in KS lesions , namely IL 1 beta , TNF alpha , and IFN gamma , synergizes with Tat to promote in nude mice the development of angioproliferative KS like lesions that are not observed with each factor alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human peripheral blood monocytes , primed with IFN gamma and GM CSF , were activated with CD 40 ligand ( CD40L ) or TNF alpha and cocultured with MC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Flow cytometric analysis of cytokine production by fresh hepatic lymphocyte subsets activated by CD 3 cross linking or PMA and ionomycin stimulation indicated that NT cells and T cells could produce IFN gamma , TNF alpha , IL 2 , and / or IL 4 , but little or no IL 5 , while NK cells produced IFN gamma and / or TNF alpha only . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Total RNA was analysed by normalized RT PCR using oligo dT as the RT primer and IL 2 , IFN gamma , IL 12 , IL 18 , IL 4 , IL 10 , TGF beta , TNF alpha and perforin specific primers for amplification . ^^^ This studied showed that Th 1 related cytokines ( IL 2 , IFN gamma , IL 12 , IL 18 , TNF alpha ) and perforin were present in most samples . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Noni ppt was also capable of stimulating the release of several mediators from murine effector cells , including tumour necrosis factor alpha ( TNF alpha ) , interleukin 1beta ( IL 1beta ) , IL 10 , IL 12 p 70 , interferon gamma ( IFN gamma ) and nitric oxide ( NO ) , but had no effect on IL 2 and suppressed IL 4 release . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although in vivo administration of LPS in pregnant mice showed a 72 % decrease of serum IL 10 , no significant difference in serum TNF alpha , IFN gamma , and IL 4 levels , compared to controls , could be detected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Optimum induction of iNOS mRNA in these cells was obtained with IFN gamma and IL 1beta treatment , while further addition of TNF alpha did not have significant effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The specific relationship among IL 1beta , TNF alpha , and IFN gamma on iNOS induction and NO synthesis in human alveolar epithelial cells has not been determined . ^^^ We exposed monolayer cultures of A 549 cells to a factorial combination of three cytokines ( IL 1beta , TNF alpha , and IFN gamma ) and three concentrations ( 0 , 5 , and 100 ng / mL ) . ^^^ Several plausible mechanisms of synergy among IL 1beta , TNF alpha , and IFN gamma in NO production from human alveolar epithelial cells ( A 549 ) are proposed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
KGF given before transplantation inhibited the T cell induced increase in bronchoalveolar lavage fluid protein , TNF alpha , IFN gamma , and nitrite levels measured on day 7 after transplantation without modifying cellular infiltration or proinflammatory cytokines and inducible . ^^^ In contrast , the same schedule of KGF failed to prevent permeability edema or suppress TNF alpha , IFN gamma , and . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) production was markedly reduced when IL 2 activated T cells were incubated with galectin 1 or its mutant . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tissue levels of IL 2 , IL 4 , IL 10 , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) were all higher in P / E / mice , but the increases were not statistically significant . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Productive proliferation is characterized by an increased secretion of various cytokines measured ( interleukin ( IL ) 2 , IL 4 , IL 6 , IL 10 , tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The pattern of these cytokines is in context with the severity of the disease because ( 1 ) IFN gamma was previously demonstrated to promote parasite growth ( 2 ) TNF alpha was previously demonstrated to kill the parasites and ( 3 ) IL 4 was previously demonstrated to promote antibody production necessary for elimination of the infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When the same MTHC lines are exposed to TNF alpha in combination with IFN gamma , the cells instead become DC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : We performed flow cytometry analysis of peripheral blood T cells during LABD , epicutaneous testing ( scratch patch ) and lymphocyte proliferation analysis ( LTT ) with the suspected drugs , routine histological and immunohistochemical examination of the acute skin lesions during LABD as well as of the positive epicutaneous test reactions and measurement of cytokines ( IL 4 , IL 5 , IL 10 , TNF alpha , IFN gamma ) in the supernatant of the LTT cultures . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A trend of increased TNF alpha and IFN gamma expression was noted in the MS relapse group , in contrast to the high ROS release in the MS remission group . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , interferon gamma ( IFN gamma ) or tumor necrosis factor alfa ( TNF alpha ) did not upregulate the expression of CD 59 on rat Schwann cells in culture . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Rat hepatocytes were incubated with indometacin ( Ind ) , Din , forskolin ( For ) , or dibutyryl cyclic GMP ( db cGMP ) in a medium containing CM ( LPS plus TNF alpha , IL 1 beta , and IFN gamma ) for 24 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This effect was associated with decreased intragraft mRNA for IFN gamma and TNF alpha and increased mRNA for IL 4 and IL 5 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Caspase 1 is transcriptionally induced during EAE , and its levels correlate with the clinical course and transcription rate of proinflammatory cytokines such as TNF alpha , IL 1beta , IFN gamma , and IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Splenic production of IL 2 , IFN gamma , and TNF was significantly suppressed from CD 154 deficient mice , indicating a lack of T cell priming . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Remarkably , the copresence in vitro of neutralizing Ab to IL 12 , but not to TNF alpha , IL 1beta , or IFN gamma , ablated the enhancing effect of CD 40 engagement on the APC function of DC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To test this hypothesis , we used bronchoalveolar lavage cells from control and CBD subjects to evaluate the beryllium salt specific production of endogenous IL 10 and the effects of exogenous human rIL 10 ( rhIL 10 ) on HLA expression , on the production of IL 2 , IFN gamma , and TNF alpha , and on T lymphocyte proliferation . ^^^ Second , the addition of excess exogenous rhIL 10 partially inhibited the beryllium stimulated production of IL 2 , IFN gamma , and TNF alpha ; however , we measured no change in T lymphocyte proliferation or in the percentage of alveolar macrophages expressing HLA DP . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This was accompanied by a reduction in the levels of Ag induced splenic T cell proliferation , decreased IFN gamma and TNF alpha synthesis , but increased IL 4 production by the mutant mice compared with the wild type mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 2 , IFN gamma , and TNF alpha mRNA expression in peripheral blood mononuclear cells in patients with multiple sclerosis ] . ^^^ A significantly higher levels of IL 2 , IFN gamma mRNA in patient with MS compared to normal controls and significantly higher level of expressions of IFN gamma , TNF alpha mRNA were detected in active MS patients compared to those with stable desease ( P < 0 . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We describe a novel mechanism of signaling interaction through which tumor necrosis factor alpha ( TNF alpha ) treatment augments interferon gamma ( IFN gamma ) induced Stat1alpha DNA binding complexes and transcriptional activation of a Stat binding element . ^^^ In TNF alpha treated cells , IFN gamma induced phosphorylation of Jak 2 kinase is increased , Jak 2 kinase activity is enhanced , and genetic studies indicate that TNF alpha requires Jak 2 kinase activity to enhance Stat1alpha tyrosine phosphorylation . ^^^ Increased Jak 2 and Stat1alpha phosphorylation are observed within minutes of coexposure to TNF alpha / IFN gamma , suggesting a direct signaling interaction . ^^^ IFN gamma receptor chain 1 ( IFNGR 1 ) tyrosine phosphorylation is markedly enhanced in cells treated with TNF alpha / IFN gamma without alteration in receptor levels . ^^^ Thus , there exists a direct signaling interaction between TNF alpha and IFN gamma , independent of cooperating enhancer elements , that may be relevant for cytokine action during immune mediated host defense and inflammatory processes . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study , we showed that Fas L expression on AK 5 cells is regulated by interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) , as injection of antibodies against IFN gamma downregulated the expression of Fas L by tumor cells as determined by immunostaining and Northern hybridizations . ^^^ These observations suggest that such cytokines as IFN gamma and TNF alpha play an important role in regulating the expression of Fas L by AK 5 cells . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study we genetically engineered betaTC tet cells with the anti apoptotic gene Bcl 2 using new lentiviral vectors and showed that it protected this cell line against apoptosis induced by hypoxia , staurosporine and a mixture of cytokines ( IL 1beta , IFN gamma and TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
At this time , both female and male IL 10 ( / ) mice produced more gamma interferon ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and IL 12p40 mRNA than their respective WT counterparts . ^^^ The data suggest that IFN gamma independent pathways may be responsible for these pathological features of P . chabaudi malaria and may be due to direct stimulation of TNF alpha by the parasite . ^^^ Since male and female knockout mice both produce more inflammatory cytokines than their WT counterparts , it is likely that the mortality seen in females is due to the nature or magnitude of the response to these cytokines rather than the amount of IFN gamma or TNF alpha produced . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of IFN gamma or TNF was protective . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We show that IFN gamma , TNF alpha and lymphotactin are transcriptionally regulated in thymocytes , whereas cytoskeletal keratin 14 , IL 1alpha and TNF alpha are regulated in the stroma , quantitatively reproducing the variations associated with beta selection of thymocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The Pfe induced TNF response at day 1 is associated with a much higher level of IFN gamma production and a much lower level of IL 12 p 40 and IL 10 expression than a comparable endotoxin induced TNF response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Venous blood samples for measurement of interleukin ( IL ) 1beta , IL 2 , IL 4 , IL 6 , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) were taken before the induction of anaesthesia , pre incisionaly , at the end of anaesthesia and surgery and 24 h postoperatively . ^^^ Pre incisionaly , in both groups , IL 1beta , IL 4 , IL 6 , TNF alpha and IFN gamma did not show a significant change , whereas IL 2 showed a significant decrease ( p < 0 . 005 in group 1 and p < 0 . 001 in group 2 ) compared with pre induction levels . ^^^ By the end of anaesthesia and surgery , IL 1beta , IL 2 , IL 4 , IL 6 and TNF alpha showed a significant increase in group 2 ( p < 0 . 005 for IL 1beta , IL 2 and IL 4 , and p < 0 . 05 for IL 6 and TNF alpha ) ; while in group 1 , only IL 2 showed a significant increase ( p < 0 . 01 ) and IFN gamma showed a significant decrease ( p < 0 . 05 ) compared with pre incisional levels . ^^^ By 24 h postoperatively , IL 1beta , IL 4 , IL 6 and TNF alpha had decreased significantly in group 2 ( p < 0 . 005 for IL 4 and p < 0 . 05 for the others ) ; whereas in group 1 , IL 2 and IFN gamma showed a significant increase ( p < 0 . 005 ) compared with the end of anaesthesia and surgery level . ^^^ In conclusion , TIVA with propofol , sufentanil and atracurium does not seem to have a significant effect on IL 1beta , IL 4 , IL 6 , TNF alpha and IFN gamma release . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
VCAM 1 was not present on resting HBECs , and was not upregulated after stimulation with IFN gamma , IL 1 , IL 4 , or TNF alpha . ^^^ PMN adhesion to HBECs could be induced either through activation of PMN with IL 8 , granulocyte macrophage colony stimulating factor ( GM CSF ) , or C5a , but not with IL 5 or by preactivation of HBECs with TNF alpha or IFN gamma . ^^^ Adherence of Eos to HBECs could be induced through activation of Eos with IL 5 , GM CSF , or C5a , but not with IL 8 or by prior activation of HBECs with TNF alpha of IFN gamma . ^^^ Maximal adhesion of Eos and PMN required pretreatment of HBECs with either TNF alpha or IFN gamma in addition to leukocyte activation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
HB MVEC prestimulated with tumor necrosis factor ( TNF ) and IFN gamma significantly promoted leukocyte transendothelial migration . ^^^ IFN beta inhibited the activated leukocyte transendothelial migration on TNF / IFN gamma activated HB MVEC in a dose dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , either anti IFN gamma , anti tumor necrosis factor ( TNF ) alpha or anti TGF beta mAb given together with Con A markedly inhibited the development of hepatic fibrosis . ^^^ Treatment with either anti IFN gamma or anti TNF alpha mAb also completely prevented hepatic injury ; in contrast , treatment with anti TGF beta mAb did not . ^^^ The treatment with anti TGF beta mAb did not affect the levels of hepatic mRNAs for either IFN gamma or TNF alpha after Con A injection . ^^^ Treatment with either anti IFN gamma or anti TNF alpha did not affect the expression levels of TGF beta in the liver . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To evaluate the contribution of cytokines ( Cks ) in the pathophysiology of preeclampsia , we investigated the plasma levels of interferon gamma ( IFN gamma ) , interleukin 6 ( IL 6 ) and tumor necrosis factor alpha ( TNF alpha ) , respectively , by an enzyme linked immunosorbent assay . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TGF beta 1 gene delivery had pronounced downregulatory effects on T cell proliferation and production of interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) , on in vitro restimulation with MBP . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In cell cultures exposed to pdFVIII , T cells showed reduced production of TNF alpha , IL 2 and IFN gamma ; monocytes showed reduced production of TNF alpha , IL 1alpha , IL 1beta , IL 6 , IL 8 and IL 12 but an increase in IL 10 synthesis ; IFN gamma synthesis by NK cells was reduced . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pretreatment of recipients with antibodies to cytokines or silica reduced lymphocyte adherence to islet endothelium from 2 . 04 % ( goat immunoglobulin G ; IgG ) or 1 . 82 % ( rat IgG ) to 0 . 47 , 0 . 58 , 0 . 39 or 0 . 19 % for monoclonal antibody ( mAb ) interferon gamma ( IFN gamma ) , polyclonal antibody ( pAb ) tumour necrosis factor alpha ( TNF alpha ) , pAb interleukin ( IL ) 1alpha or silica , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) are cytokines that can individually or additively suppress thyroid cell function and the expression of thyroid specific genes , such as thyroglobulin ( TG ) and thyroperoxidase ( TPO ) . ^^^ In the present study , we examine the effects of TNF alpha and IFN gamma on TTF 2 gene expression , as well as the DNA binding activity of TTF 2 . ^^^ FRTL 5 cells were maintained in 5H medium containing 0 . 2 % calf serum for 7 days , then incubated with TNF alpha , IFN gamma , or TNF alpha plus IFN gamma . ^^^ TNF alpha ( 50 ng / ml ) only slightly suppressed ( 61+ / 2 % compared with control ) , whereas IFN gamma ( 100 U / ml ) modestly decreased TTF 2 messenger RNA ( mRNA ) levels ( 34+ / 4 % ) . ^^^ TNF alpha and IFN gamma simultaneously caused a marked decrease in TTF 2 mRNA levels ( 13+ / 2 % ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Previous studies have shown that CD 4 ( + ) T cells , IL 5 , and TNF alpha mediate , but IFN gamma and IL 12 ( via IFN gamma production ) inhibit antigen induced eosinophil recruitment into the airways of sensitized mice . ^^^ The administration of IL 18 enhanced antigen induced IFN gamma and TNF alpha production , but not IL 5 production , in the BALF and lungs of sensitized mice . ^^^ These results indicate that IL 18 , unlike IFN gamma and IL 12 , enhances antigen induced eosinophil recruitment into the airways in part by increasing antigen induced TNF alpha production of sensitized animals . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found that fibroblasts produced increased amounts of uPA protein after exposure to a FGF , b FGF , EGF , PDGF BB , and IFN gamma , were unaffected in this respect by IL 6 , M CSF , GM CSF and Oncostatin M , and produced decreased amounts of uPA protein after exposure to IL 1alpha , TNF alpha , IGF 1 , and IGF 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A prospective study was conducted with Bangladeshi children with rotavirus ( RV ) diarrhea to assess whether nutritional and clinical parameters , RV serotypes , levels of interleukin 10 ( IL 10 ) , tumor necrosis factor alpha ( TNF alpha ) , and gamma interferon ( IFN gamma ) , and RV specific antibody titers in plasma and stool were associated with the development of persistent diarrhea . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
EC expressed MCP 1 mRNA and protein in response to TNF alpha , IFN gamma or IL 1beta , but not TGF beta 1 . ^^^ TGF beta 1 in cotreatment with either TNF alpha or IL 1beta , but not IFN gamma , significantly decreased MCP 1 mRNA and protein expression , as compared to TNF alpha or IL 1beta treatment alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The enzyme linked immunosorbent assay technique was used to determine levels of urinary cytokines interleukin ( IL ) 2 , IL 4 , IL 6 , IL 8 , IL 10 , IL 12 , tumor necrosis factor ( TNF ) , human granulocyte macrophage colony stimulating factor ( hGM CSF ) , IL 1beta , and interferon gamma ( IFN gamma ) . ^^^ RESULTS : Cytokines IL 4 , IL 10 , IL 12 , TNF , hGM CSF , IL 1beta , and IFN gamma were not detected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Out of 15 cytokines tested for induction or enhancement of MCP 1 secretion , interleukin 3 ( IL 3 ) , IL 6 , interferon gamma ( IFN gamma ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , macrophage colony stimulating factor ( M CSF ) and tumor necrosis factor ( TNFalpha ) were able to augment ( twofold to 12 fold ) the MCP 1 level in the culture medium of MONO MAC 6 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Their effects overlap with regard to the inhibition of the pro inflammatory cytokines TNF alpha , IFN gamma , IL 12 , and the stimulation of production of the major anti inflammatory cytokine IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These cells were exposed for different time points to IL 1beta ( 50 U / mI ) , IFN gamma ( 1 , 000 U / ml ) and / or TNF alpha ( 1 , 000 U / ml ) before being harvested for determination of viability ( by nuclear dyes ) and mRNA expression ( by RT PCR with specific primers ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , bacteria promoted in a dose dependent manner DC release of cytokines ( IL 6 , TNF alpha , IL 1beta , IL 12 , TGF beta , and IL 10 ) and of the chemokines IL 8 , RANTES , IFN gamma inducible protein 10 , and monokine induced by IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This microbe induced and T cell independent response depends on both macrophages ( MPhi ) and NK cells : HKL stimulated MPhi release TNF alpha and IL 12 , which together activate NK cells for IFN gamma release . ^^^ HKL induced IL 12 , TNF alpha , and IFN gamma in SCID whole spleen cell cultures was inhibited by coincubation with anti F4 / 80 mAb whereas IL 1 and IL 10 were enhanced . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The reactivation of CIA by oral administration of LPS was associated with increase in anti type 2 collagen IgG and IgG2a Abs as well as varying kinds of cytokines including IL 12 , IFN gamma , IL 1beta , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Amelioration of experimental allergic neuritis by sodium fusidate ( fusidin ) : suppression of IFN gamma and TNF alpha and enhancement of IL 10 . ^^^ The beneficial effects were associated with profound modifications of the capacity of these rats to produce and release pro and anti inflammatory cytokines such as IFN gamma , TNF alpha and IL 10 , which are important in regulating the development of EAN . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment with imiquimod , an immune response modifier , stimulated significant increases in mRNA for interferon ( IFN ) alpha , IFN gamma and 2 ' , 5 ' oligoadenylate synthetase ( 2 ' , 5 ' AS ) as well as a tendency towards increases in tumor necrosis factor ( TNF ) alpha and interleukin 12 p 40 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of C 9 mRNA was also induced upon stimulation by interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) and / or lipopolysaccharide ( LPS ) . ^^^ Northern blot analysis showed that the mRNA expression of C 3 , C 4 , DAF and Crry was up regulated , but that of CD 59 was down regulated by IFN gamma , TNF alpha and / or LPS alone or by synergy . ^^^ The increase of C 3 mRNA by TNF alpha or LPS and that of C 4 mRNA by IFN gamma was induced in a dose dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the immunophenotype of circulating lymphocytes , the expression / release of CD 30 ( a member of the tumor necrosis factor receptor family preferentially associated with T helper 2 type immune responses ) and the intracellular patterns of interferon gamma ( IFN gamma ) , Interleukin 2 ( IL 2 ) , IL 4 , IL 5 , and IL 10 production by T cell subsets , as evaluated by single cell flow cytometric analysis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The first is an effort to induce antitumor immunity by enriching the cytokine environment within the primary cancer by intraprostatic injection of Leukocyte Interleukin ( Cel Sci Corp , Vienna , VA ) , a mixture of natural cytokines that includes interleukin 1 beta ( IL 1beta ) , IL 2 , granulocyte macrophage colony stimulating factor ( GM CSF ) , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We show that in vitro established OVA and beta GAL specific CTL clones release TNF alpha and IFN gamma when incubated with recombinant gp 96 irrespective of the antigenic peptide sequences hooked to the C terminus of gp 96 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In other experiments , cells were treated for 48 hours with various cytokines including interleukin 6 ( IL 6 ) ( 100 U / mL ) , interferon gamma ( IFN gamma ) ( 100 U / mL ) , tumor necrosis factor alpha ( TNF alpha ) ( 10 ng / ml ) , IL 1alpha ( 100 U / mL ) , and IL 1beta ( 100 U / mL ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Molecular analysis of skin biopsies of patients with psoriasis before and during rhIL 11 treatment demonstrates a decrease in mRNA levels of TNF alpha , IFN gamma and iNOS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In Crohn ' s disease , there appears to be an excessive T ( H ) 1 T cell response to an antigenic stimulus , leading to increased levels of proinflammatory cytokines , such as interferon gamma ( IFN gamma ) , interleukin ( IL ) 12 , IL 1 , IL 6 , and tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The exhausted medium was assayed for cytokines interleukin 6 ( IL 6 ) , IL 8 , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) by ELISA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When the cells were stimulated with IFN gamma , tumor necrosis factor alpha ( TNF alpha ) , or LPS in combinations , only the combination of IFN gamma and LPS produced more NO than that produced by IFN gamma alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Murine peritoneal M & phi ; simultaneously stimulated with fgEPO and one of the following , ( 1 ) LPS , ( 2 ) mannosylated bovine serum albumin ( mBSA ) , ( 3 ) interferon gamma ( IFN gamma ) , or ( 4 ) Poly 1 : C , demonstrated both dose and time dependent decreases in TNF alpha and IL 6 and a dose dependent decrease in IFN alpha / beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Chronic LED plus BCG infection further increased TNFalpha ( p = 0 . 002 ) and IFN gamma ( p = 0 . 04 ) ; IL 10 was unchanged , whereas IL 4 was marginally further decreased ( p = 0 . 06 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Effects of TNF alpha , IFN gamma and IL beta on normal human bronchial epithelial cells . ^^^ To investigate the possible relation between cytokines and epithelial damage , the effects of tumour necrosis factor alpha ( TNF ) alpha , interferon gamma ( IFN gamma ) and interleukin 1 beta ( IL 1beta ) on normal human bronchial epithelial cells in vitro were studied . ^^^ IFN gamma and TNF alpha generally potentiate each other ' s effects . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TGF beta 1 DNA not only reduced key proinflammatory cytokines including TNF alpha , IL 1 beta , IFN gamma , and IL 18 , but also inhibited both CXC and CC chemokine production , thereby blocking inflammatory cell recruitment and accumulation in the liver . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our results demonstrated that CD40L was expressed on freshly isolated lamina propria T cells from these patients and was functional to induce IL 12 and TNF production by normal monocytes , especially after IFN gamma priming . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Enzyme linked immunospot ( ELISPOT ) assays were adopted to determine levels of IFN gamma , TNF alpha , IL 6 and IL 10 secreting DC vs . mononuclear cells ( MNC ) . ^^^ Patients with MS had higher levels of IFN gamma , TNF alpha and IL 6 secreting DC than healthy subjects . ^^^ The differences for IFN gamma and TNF alpha secreting cells were confined to the subgroup of untreated MS patients and not observed in the subgroup examined during ongoing treatment with IFN beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The production of IL 1beta , IL 6 , IFN gamma , TNF alpha and TNF beta markedly decreased during 24 48 h after each injection , with limited dose dependency and no evidence of tolerance or effect augmentation over 1 month . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Foals infected intrabronchially with a virulence plasmid containing strain of R . equi had similar gamma interferon ( IFN gamma ) and interleukin 12 ( IL 12 ) p 35 but significantly higher IL 1beta , IL 10 , IL 12 p 40 , and tumor necrosis factor alpha ( TNF alpha ) mRNA expression in lung tissue compared to foals infected with the plasmid cured derivative . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using FACScan analysis , we have shown that restimulation with live M . bovis BCG induced more CD8+ T cell activation than the soluble antigen purified protein derivative and that these cells are actively producing the type 1 cytokines IFN gamma and tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To understand the cytokines induced during this infection we determined the levels of interleukin ( IL ) 1beta , IL 2 , IL 4 , IL 8 , IL 12 , tumor necrosis factor of ( TNF alpha ) and interferon gamma ( IFN gamma ) in serum of patients with brucellosis and compared with those without brucellosis and controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pathological analysis was performed and measurements of endotoxin were taken , lipid peroxidation , NF kappaB , and messenger RNA ( mRNA ) levels of proinflammatory cytokines ( tumor necrosis factor alpha [ TNFalpha ] , interleukin 1 beta [ IL 1beta ] , interferon gamma , [ IFN gamma ] , and IL 12 ) , C C chemokines ( regulated upon activation , normal T cell expressed and secreted [ RANTES ] , monocyte chemotactic protein [ MCP ] 1 , macrophage inflammatory protein [ MIP ] 1alpha ) , C 10 C chemokines ( cytokine induced neutrophil chemoattractant ( CINC ) , MIP 2 , IP 10 , and epithelial neutrophil activating protein [ ENA ] 78 ) , and anti inflammatory cytokines ( IL 10 , IL 4 , and IL 13 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It inhibits IL 2 and IFN gamma production from the T cells and downregulates the expression of TNF alpha in the antigen presenting cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Commercial sandwich enzyme linked immunosorbent assay kits were used to assay the levels of tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , interleukin ( IL ) 2 , IL 4 , IL 5 , IL 6 , and IL 10 in culture supernatants . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Therefore , we investigated the effect of several decontamination techniques on the immunoreactivity and bioactivity of the inflammatory cytokines IL 1beta , IL 6 , TNF alpha , IL 12 , and IFN gamma , the anti inflammatory cytokine IL 10 , and the chemokine IL 8 in biological specimens . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Retinoic acid ( RA ) , and the combination of TNFalpha and Interferon ( IFN gamma ) inhibit human salivary gland tumor ( HSG ) cell growth with the combination of all three being even more inhibitory ( P < 0 . 05 ) . ^^^ Previous studies have demonstrated that these inhibitory effects of RA , and the combination of TNFalpha and IFN gamma are associated with increased accumulation of IGFBP 3 in the culture medium of HSG cells . ^^^ TNFalpha and IFN gamma induced a rise in IGFBP 3 mRNA levels between 4 and 8 h , which returned to control levels after 24 h . ^^^ When antibodies to IGFBP 3 were used with TNFalpha and IFN gamma , the inhibitory effect of the cytokines on cell growth was diminished . ^^^ Retinoic acid with TNFalpha and IFN gamma had a marked inhibitory effect ( P < 0 . 05 ) which was similarly reversed by increasing concentrations of IGFBP 3 antibody . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The vast majority of resting CD 8 ( pos . ) T cells capable of rapid induction of IFN gamma and TNF alpha synthesis in response to HCMV peptides were found in a subset characterized by intermediate to high expression of CD 57 , down regulation / loss of CD 27 , and varying degrees of reversal of the classical `` memory ' ' CD45RO ( bright ) / RA ( dim ) phenotype . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A soluble factor produced by lamina propria mononuclear cells is required for TNF alpha enhancement of IFN gamma production by T cells . ^^^ A correlation between TNF alpha and elevated IFN gamma production is suggested by the reduction in the number of IFN gamma producing lamina propria mononuclear cells ( LPMC ) found in colonic biopsies from anti TNF alpha treated patients . ^^^ The aim of this study was to define the mechanism of TNF alpha augmented mucosal T cell IFN gamma production . ^^^ In this paper we present evidence that cultured LPMC secrete a factor which acts on preactivated T cells in concert with TNF alpha to augment IFN gamma production . ^^^ Peripheral blood PHA activated T cells incubated in supernatants from LPMC became responsive to TNF alpha by increasing IFN gamma output upon stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , IL 1beta and TNF alpha did not display frequency doubling with or without IFN gamma exposure . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Examination of accessory cell functions associated with resistance to T . gondii revealed that RelB / macrophages stimulated with IFN gamma plus LPS or TNF alpha produced IL 12 as well as reactive nitrogen intermediates and inhibited parasite replication similar to WT macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Results of experiments conducted in parallel using mAbs to IL 15 , as well as to other cytokines ( IL 2 , IL 12 , IFN gamma , and TNF alpha ) , clearly indicated that IL 15 was specifically responsible for the observed effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma can promote production of TNF and can synergize with this cytokine in its actions on responder cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The modulatory effect of the cytokines released in the skin , such as IL 1beta , GM CSF , and TNF alpha as epidermal cytokines , IL 2 , IL 12 , and IFN gamma as Th 1 type cytokines , and IL 4 and IL 10 as Th 2 type cytokines , was analyzed using the chemotactic chamber method in this study . ^^^ Both GM CSF and TNF alpha induced the migration of human Langerhans cells in vitro , whereas IL 1beta , IL 2 , IL 10 , IL 12 , and IFN gamma had no effect on Langerhans cell migration . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Differential selectivity of CIITA promoter activation by IFN gamma and IRF 1 in astrocytes and macrophages : CIITA promoter activation is not affected by TNF alpha . ^^^ Importantly , while TNF alpha is able to synergize with IFN gamma to increase astrocyte MHC class 2 expression in vitro , we show that treatment of rat astrocytes with TNF alpha has no effect on CIITA promoter activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These data confirm that TNF or Lt alpha is a key cytokine for the anticryptococcal response and demonstrate its major role for the induction of IL 1beta , IL 6 , and KC in the brain ; however , its presence is not a prerequisite for IL 12 , IFN gamma , and nitrite / nitrate production . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Levels of IL 2 , IL 6 , IFN gamma , TNF alpha and GM CSF were increased after OKT 3 infusion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MS is associated with a cytokine storm characterized by the parallel upregulation of proinflammatory ( IFN gamma , TNF alpha , and beta , and IL 12 ) and immune response down regulating ( TGF beta , IL 10 ) cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Constitute release of RANTES was low , ranging from 28 52 ng / mL but addition of interferon gamma ( INF gamma ) or the combination of IFN gamma and tumor necrosis factor alpha ( TNF alpha ) produced a marked increase in RANTES production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The levels in serum of ( 1 ) the bone stimulating peptide insulin like growth factor 1 ( IGF 1 ) , ( 2 ) the inhibitor of bone resorption interferon gamma ( IFN gamma ) , ( 3 ) the stimulators of bone resorption interleukin 1 beta ( IL 1 beta ) tumor necrosis factor alpha ( TNF alpha ) and interleukin 6 ( IL 6 ) were evaluated by immunoassay . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Thus , the objective of this study was to determine whether major splenocyte derived cytokines interleukin 2 ( IL 2 ) , interleukin 4 ( IL 4 ) , interferon gamma ( IFN gamma ) and macrophage derived cytokines interleukin 1beta , ( IL 1beta ) , interleukin 6 ( IL 6 ) , and tumor necrosis factor alpha ( TNF alpha ) were affected by ergotamine . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Subsequently , jejunal iNOS expression was detected by immunohistochemistry , ion transport was evaluated in Ussing chambers , and serum levels of TNF alpha and IFN gamma were measured by ELISA . ^^^ The prolongation of epithelial ion transport abnormalities correlated with elevated serum levels of TNF alpha and IFN gamma in mice treated 24 h previously with l NIL plus SEB compared with those in controls and SEB only treated mice . ^^^ Additionally , mice treated with l NIL plus SEB and TNF alpha or IFN gamma neutralizing Abs displayed normal jejunal ion transport characteristics 24 h posttreatment . ^^^ We conclude that NO mobilization is important in the homeostatic recovery response following immune stimulation by SAgs and that the beneficial effect of NO in this model system is probably via regulation of TNF alpha and IFN gamma production . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma and TNF alpha , as well as gut derived hsp 60 , were elevated at sites of T cell infiltration . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Therefore we pretreated the recipients with antibodies to cytokines or silica . mAb IFN gamma , pAb TNF alpha , pAb IL 1alpha or silica reduced lymphocyte adherence to islet endothelium significantly ( P < 0 . 01 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro experiments supported these results and showed that IL 1beta , TNF alpha , and CD 40 ligand potentiated IP 10 expression from IFN gamma stimulated ECs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Production of TARC by keratinocytes was confirmed by culturing murine keratinocytic cell line cells ( PAM 212 ) with TNF alpha , IFN gamma , or IL 1beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) induces apoptosis in many tumor cell lines and sensitizes tumor cells to apoptosis by tumor necrosis factor family members . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Enhancement of IL 10 production by MIP 3beta correlated with the inhibition of IL 12 p 40 and TNF alpha production by monocytes and with the impairment of IFN gamma production by T cells , which was reversed by addition of anti IL 10 Abs to the cultures . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inducible NO synthase ( iNOS ) mRNA was found in diseased liver tissue along with TNF alpha and IFN gamma , which are known promoters of iNOS expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lungs of mice given mAb to GM CSF manifested depressed levels of TNF alpha , IFN gamma , and reactive nitrogen intermediates and elevated levels of IL 4 and IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mincle mRNA expression was strongly induced in response to several inflammatory stimuli , such as LPS , TNF alpha , IL 6 , and IFN gamma in wild type macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Other monokines such as IL 1beta , IL 6 , TNF alpha neither reversed the inhibitory effect of P ExA nor induced production of IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These in vivo observations were related to in vitro data : cytokines ( IL 1beta , IFN gamma , and TNF alpha ) induced NOSII expression in human MCF 7 breast cancer cells , and NO inhibited their proliferation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Activation by lipopolysaccharide ( LPS ) of PBMC previously incubated with ibandronate led to enhanced levels of tumour necrosis factor alpha ( TNF alpha ) secretion , and this could be partially inhibited by neutralizing antibodies to IFN gamma . ^^^ CONCLUSIONS : The enhanced production of TNF alpha by ibandronate treated PBMC in vitro involves stimulation of adherent monocytes by IFN gamma prior to LPS induced activation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Addition of graded doses of IL 12 with anti CD 3 resulted in a significant increase in both IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This compound inhibited EAE induced spinal cord mononuclear cell invasion and normalized tumor necrosis factor alpha and IFN gamma mRNA expression within the spinal cord . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Unstimulated baseline BAL T cells expressed mRNA for IFN gamma , IL 13 , and TNF alpha . ^^^ Both IL 4 and IL 3 were strongly upregulated after PHA stimulation , while the expression of TNF alpha and IFN gamma was reduced , compared to equivalent baseline samples . ^^^ CONCLUSIONS : T cells from asthmatic airways produce IL 13 , IFN gamma , and TNF alpha , but after allergen challenge , type 2 cytokines are upregulated . mRNA and protein analysis provide complementary information on airways T cell cytokine profiles . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro infection by C pneumoniae of U 937 macrophages stimulated the production of IL 1beta , IFN gamma , and TNF alpha in tissue culture . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The influence of pharmacological treatment on the immune response of patients with Echinococcus granulosus infection was evaluated by reverse transcriptase polymerase chain reaction ( RT PCR ) to determine mRNA expression for IL 12 p 35 , IL 12 p 40 , interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) and IL 4 in PBMC from 12 patients before and after chemotherapy and from seven uninfected controls . ^^^ Most patients ' PBMC showed measurable amounts of IL 12 p 35 , IL 4 , IFN gamma and TNF alpha mRNA in parasite antigen stimulated and unstimulated cultures . ^^^ IFN gamma and TNF alpha mRNA expression increased in patients who responded to therapy and decreased in patients who did not . ^^^ In contrast to IL 12 p 35 , IFN gamma and TNF alpha mRNAs , IL 12 p 40 and IL 4 mRNAs were detected exclusively in patients , suggesting a close relationship between these two cytokines and cystic echinococcosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We observed that low doses of MTX strongly suppress TNF and to a lesser extent interferon gamma ( IFN gamma ) production by T cells from both healthy donors and RA patients when present during T cell priming via the TCR . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Bacterial CW are capable of inducing production of a proinflammatory cytokine , tumour necrosis factor alpha ( TNF alpha ) , and an anti inflammatory cytokine , IL 10 , but not that of IL 4 or interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
LPS was the most potent stimulus of IL 1beta and TNF alpha production , followed by LPS + IFN gamma and IFN gamma in decreasing order . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study , the sensitivity of LMVEC and human umbilical vein endothelial cells ( HUVEC ) to lipopolysaccharide ( LPS ) and the proinflammatory cytokines IL 1 , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) was compared . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To understand the dynamic nature of the immune response in leprosy , we examined the relationship between circulating Mycobacterium leprae specific antibodies and secreted cytokines [ interferon gamma ( IFN gamma ) , interleukin 2 ( IL 2 ) , IL 5 , IL 10 , IL 6 , tumour necrosis factor alpha ( TNF alpha ) and granulocyte macrophage colony stimulating factor ( GM CSF ) ] in leprosy patients ( 19 lepromatous patients ; 25 tuberculoid patients ) and their exposed household contacts ( HC=14 ) in response to M . leprae antigens . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After the combined treatment with IFN gamma and lipopolysaccharide ( LPS ) , production of NO by wild type ( wt ) and TNF / LT alpha ( / ) BMDM was induced to comparable levels , but only wt BMDM controlled L . monocytogenes growth efficiently . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression could be further increased by activating the cells with a combination of tumor necrosis factor a ( TNF alpha ) , IL 1beta , and IFN gamma . ^^^ MC stimulated with a combination of TNF alpha , IL 1beta , and IFN gamma , but not unstimulated MC , migrated toward a RANTES gradient . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
On admission serum cytokine levels of interleukins 4 and 10 ( IL 4 , IL 10 ) , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) were grossly raised in comparison with a matched control group ( P < 0 . 001 ) . ^^^ Levels of IL 4 , IL 10 and TNF alpha fell ( P < 0 . 001 , P < 0 . 01 and P < 0 . 01 , respectively ) and levels of IFN gamma rose more ( P = 0 . 005 ) in immunotherapy recipients than in those receiving chemotherapy alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Zinc therapy improved sperm quality and increased seminal IL 4 , but reduced TNF alpha and IFN gamma . ^^^ Cadmium had a linear correlation with TNF alpha and IFN gamma , but not with IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here we show that tumor necrosis factor alpha ( TNF alpha ) in addition to interferon gamma ( IFN gamma ) induces cell surface expression of functional FasL in human HT 29 colon and MCF 7 breast adenocarcinoma cells that constitutively lack cell surface expression of FasL . ^^^ These results demonstrate that TNF alpha and IFN gamma induce expression of functional FasL in adenocarcinoma cells which thereby can kill T lymphocytes by the FasL / Fas pathway . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Levels of interleukin 1 beta ( IL 1 beta ) , IL 2 , IL 4 , IL 6 , interferon gamma ( IFN gamma ) and TNF alpha were determined by ELISA two weeks after immunization of the mice . ^^^ Levels of IL 4 , IL 6 and TNF alpha but not IL 1 beta , IL 2 and IFN gamma were significantly elevated in the mice 2 weeks after immunization with IgG ANCA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These mice produce a very early IFN gamma spike ( 8 h after infection ) and an aberrant tumor necrosis factor alpha ( TNF alpha ) spike ( day 2 after infection ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The C57BL / 6 mice , in contrast , produced a delayed but sustained response in the lung correlating with granuloma onset and characterized by high induction of IL 6 , IFN gamma , IL 1beta , IL 10 , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cells stimulated with IL 12 and IL 18 in combination , but not with IL 2 or IL 15 , produced IFN gamma , IL 3 , IL 6 and TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Unstimulated HUVECs did not produce detectable amounts of TNF alpha , but IFN gamma , IL 1beta , and LPS when added together induced TNF alpha production of HUVECs in a time dependent manner . ^^^ IFN gamma , IL 1beta , or LPS alone did not induce TNF alpha production , whereas IFN gamma and IL 1beta in combination were able to induce TNF alpha production to some extent , and the production could be further increased with LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The IL 6 levels were measured by ELISA in serum free culture medium before and after stimulation of monolayer fibroblasts with various concentrations of tumour necrosis factor alpha ( TNF alpha ) , platelet derived growth factor ( PDGF ) , and interferon gamma ( IFN gamma ) , alone and in different combinations . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation of enriched LC with CD 40 ligand transfected cells and IFN gamma revealed that the release of IL 1beta , IL 6 , IL 8 , and TNF alpha was enhanced by UVB . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The insensitivity to Fas mediated apoptosis was not affected by priming with IFN gamma and / or TNF alpha or by blocking the DC survival signals TNF related activation induced cytokine and CD40L . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Eotaxin and MCP 4 mRNA expression induced by TNF alpha alone or in combination with IFN gamma was near maximal after 1 h , peaked at 4 and 8 h , respectively , remained unchanged up to 24 h , and was protein synthesis independent . ^^^ IL 4 and IFN gamma had selective effects on chemokine expression ; IL 4 selectively up regulated the expression of eotaxin and MCP 4 and potentiated TNF alpha induced eotaxin , while IFN gamma markedly potentiated only the TNF alpha induced expression of RANTES . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CONCLUSION : Increased IFN gamma production resulting from HBcAg specific T helper lymphocyte type 1 response , and increased TNF alpha production may contribute to cell mediated antiviral immune response in children with chronic hepatitis B . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It has been shown that stimulated lymphocytes from UCB have reduced production of cytokines including interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) , which play a role in GVHD pathophysiology . ^^^ Our observation of minimal NFAT 1 expression after stimulation correlated with reduced cytoplasmic IFN gamma and TNF alpha production in UCB T cells studied simultaneously . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A Th 1 response is characterized by the presence of IL 2 , IL 12 , TNF and IFN gamma , whereas a Th 2 response is characterized by IL 4 , IL 5 , IL 6 and IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this article , Remi Fagard and colleagues discuss the properties of neurons that lead to their low infection by Toxoplasma gondii , and the role that cytokines such as tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) might play . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Following challenge with the combination of inflammatory cytokines IL 1beta , TNF alpha , and IFN gamma , such cultures exhibit a time dependent increase in inducible NO synthetase induction and corresponding NO production , an effect which was inhibited by L NMMA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To clarify the role played by prostaglandins in acute brain inflammation we studied the expression of the key enzyme in their formation , cyclooxygenase 2 ( COX 2 ) , following microinjection of bacterial endotoxin ( LPS ) , interleukin 1beta ( IL 1beta ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) , in the rat dorsal hippocampus . ^^^ Microinjection of IFN gamma did not alter COX 2 ir , whereas TNF alpha or IL 1beta injection induced a moderate COX 2 ir in the perivascular cells of a few blood vessels close to the injection site , and in very few of the infiltrating neutrophils . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To characterize the lymphocytic thyroiditis ( LT ) of BB / W rats further and to evaluate early increases of cytokine gene expression in LT , we investigated the interleukin ( IL ) 2 , 4 , 6 , 10 , 12p40 , interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) gene expression in the thyroids , spleens , and livers of BB / W rats with and without histological evidence for thyroiditis . ^^^ The number of rats with detectable TNF alpha , IFN gamma , IL 2 , IL 12p40 gene expression was significantly higher in rats with LT . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha and IFN gamma synthesis was suppressed by IL 10 and TGF beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : IFN beta inhibited the proliferation of established MBP reactive T cell clones , which correlated with enhanced production of anti inflammatory interleukin ( IL ) 4 and IL 10 , and a decrease in tumor necrosis factor alpha ( TNF alpha ) and IFN gamma . ^^^ Although the level of TNF alpha and IFN gamma was generally unaltered or decreased , IFN beta appeared to enhance the production of IFN gamma in PBMCs derived from some individuals with MS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To investigate a potential role of MCP 3 in vascular injury , the present work examined its mRNA expression in rat aortic SMCs stimulated with various inflammatory stimuli including LPS , TNF alpha , IL 1beta , IFN gamma and TGF beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Addition of IFN gamma or TNF alpha to THP 1 derived macrophage did not effect on cytolytic activities nor apoptosis induction . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The spontaneous , unstimulated and mitogen induced production of TNF alpha and interferon gamma ( IFN gamma ) by isolated peripheral blood leukocytes was evaluated . ^^^ TNF alpha and IFN gamma production of PBMC was significantly lower in infants with cow ' s milk allergy than in healthy children . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The src family selective tyrosine kinase inhibitor PP 1 blocks LPS and IFN gamma mediated TNF and iNOS production in murine macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study , we investigated the effect of PGF 2 alpha on intercellular adhesion molecule 1 ( ICAM 1 ) expression in human gingival fibroblasts ( HGF ) and the effect of PGF 2 alpha on ICAM 1 expression elicited by proinflammatory cytokines , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) in the cells . ^^^ IFN gamma elicited ICAM 1 expression was synergistically increased by PGF 2 alpha , whereas TNF alpha induced ICAM 1 expression was slightly inhibited by PGF 2 alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using unseparated blood leukocytes , we found that the spontaneous production of IL 6 ( 4 8 h ) and transforming growth factor ( TGF ) beta 1 ( 2 4 h ) was significantly decreased and that the in vitro stimulated production of IFN gamma ( 2 8 h ) and TNF ( 4 h ) was significantly increased compared with control . ^^^ Our data concerning the increased IFN gamma and TNF producing capacity of unseparated leukocytes during pharmacologically induced hyperprolactinemia strongly support the possibility that the lymphocyte production of these cytokines can be rapidly amplified by prolactin via a priming mechanism . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro , human hepatic sinusoidal endothelial cells secreted IFN inducible protein 10 and monokine induced by IFN gamma in response to stimulation with IFN gamma in combination with either IL 1 or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Transcripts for the following cytokines were detected in patient specimens that also contained the IL 10 producing monocytes IL 2 ( 12 of 17 specimens ) , GM CSF ( 9 of 17 specimens ) , IFN gamma ( 6 of 17 specimens ) , and TNF alpha ( 4 of 17 specimens ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A CD8+ T cell line and TCR alphabeta+ T cell clone were isolated that secreted IFN gamma and TNF alpha in response to autologous prostate cancer cells but not to autologous fibroblasts or lymphoblastoid cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increased expression of TNF alpha mRNA was observed mainly in macrophages and CD 8 ( + ) T cells , and the increase in IFN gamma mRNA was observed mainly in CD 8 ( + ) T cells in BALF at Day 3 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A dose dependent reduction in serum levels of liver enzymes , tumor necrosis factor alpha ( TNF alpha ) , and IFN gamma corresponded with this amelioration of liver damage . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , after 18 h , TNF alpha and IFN gamma levels were ( strongly ) decreased , whereas the IL 10 levels were increased . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study , we compared the effects of the classical chemoattractants FMLP , PAF and LTB 4 , of the chemokine IL 8 and of TNFalpha , GM CSF , IFN gamma and IL 1beta on C5a induced chemotaxis , degranulation , oxidative burst and expression of adhesion molecules of human neutrophils in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In light of this background , we decided to measure serum levels of IL 1 beta , IL 1ra , TNF alpha and IL 6 in LL and TL patients , and we also studied the production in vitro of Th 1 ( IFN gamma , IL 2 ) , Th 2 ( IL 4 , IL 10 ) and TNF alpha cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In macrophages , Ser 727 phosphorylation in response to bacterial lipopolysaccharide ( LPS ) , UV irradiation , or TNF alpha occurred through a signaling path sensitive to the p 38 mitogen activated protein kinase ( p 38 MAPK ) inhibitor SB 203580 whereas IFN gamma mediated Ser 727 phosphorylation was not inhibited by the drug . ^^^ Furthermore , LPS , UV irradiation , and TNF alpha caused activation of p 38 MAPK whereas IFN gamma did not . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In mice treated with these mAbs , the production of TNF alpha and IFN gamma in the spleen cells was decreased . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To investigate the potential immunomodulatory effects of this virus , we performed an in vitro study in which we investigated effects of HHV 7 on the synthesis of several key immunomodulatory cytokines , i . e . tumor necrosis factor alpha ( TNF alpha ) , interleukin 2 ( IL 2 ) , interferon gamma ( IFN gamma ) , IL 4 , IL 6 , and transforming growth factor beta ( TGF beta ) . ^^^ We found elevated levels of TNF alpha , TGF beta , and IFN gamma in the supernatants of HHV 7 infected cells . ^^^ By reverse transcriptase polymerase chain reaction ( RT PCR ) analysis , using cytokine specific primers , we found that levels of TNF alpha , TGF beta , and IFN gamma mRNA were increased in the infected cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These results suggest that CWS delays regulation and control of intracerebral Toxoplasma gondii during acute infection in BALB / c mice by decreasing the early expression of IFN gamma , IL 2 , TNF alpha , iNOS , IL 1beta , and IL 12 , while increasing the expression of IL 6 , a counterregulatory cytokine . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In CD 1 modulated mice , Mycobacterium tuberculosis specific production of the type 1 cytokines , IL 12 , TNF , and IFN gamma as well as of TGF beta was reduced . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The increased resistance to infections as compared to wild type mice was associated with upregulation of innate and acquired antifungal Th 1 responses , such as a dramatically higher production of IL 12 , nitric oxide ( NO ) and TNF alpha as well as IFN gamma by CD4+ T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The proliferation of immortalized vascular endothelial cells ( IVEC ) , as measured by [ methyl 3H ] thymidine incorporation , was compared to that of HUVEC in the presence of endothelial cell growth factor and cytokines : tumor necrosis factor alpha ( TNF alpha ) , interleukin 1beta ( IL 1beta ) and interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
BACKGROUND / AIMS : Proinflammatory cytokines including tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) play a critical role in the pathogenesis of liver injury induced by lipopolysaccharide ( LPS ) or staphylococcal enterotoxin B ( SEB ) in D galactosamine ( GalN ) sensitized mice . ^^^ Serum levels of TNF alpha and IFN gamma were measured and the expressions of TNF alpha and IFN gamma mRNA in the liver and spleen were determined by reverse transcription polymerase chain reaction . ^^^ IL 10 also inhibited increases in serum TNF alpha and IFN gamma concentrations with either toxin . ^^^ Treatment with IL 10 significantly reduced TNF alpha mRNA and IFN gamma mRNA expression in the liver and spleen after administration of either toxin to sensitized mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Reverse transcriptase polymerase chain reaction ( RT PCR ) analysis revealed no difference in the production of inflammatory cytokines such as IL 1beta , IL 2 , TNF alpha , and IFN gamma in the CNS of IL 6 deficient mice with EAE as compared to the CNS of wild type mice with EAE . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Upon incubating cultures with proinflammatory cytokines ( TNF alpha , IL 1beta and IFN gamma ) or with lipopolysaccharide ( LPS ) , CD 40 expression was increased . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interestingly , cells of resistant genotype secreted more nitric oxide , TNF alpha and IL 12 , but less IL 6 , than susceptible cells , when stimulated with IFN gamma alone or in combination with lipopolysaccharide . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It has recently been shown that several PBMN derived cytokines , including transforming growth factor beta ( TGF beta ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 2 ( IL 2 ) , and interferon gamma ( IFN gamma ) may modify intestinal epithelial cell function , resulting in rapid improvement of wound repair . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Circulating concentrations and lipopolysaccharide ( LPS ) or phytohaemagglutinin ( PHA ) stimulated whole blood production of the proinflammatory cytokines IL 1beta , IL 1ra , tumour necrosis factor alpha ( TNF alpha ) and IL 10 and IFN gamma were measured . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : The production of IL 8 from cultured A 549 epithelial cells was assayed and neutrophil adherence assay in the presence or absence of interferon gamma ( IFN gamma ) plus tumor necrosis factor alpha ( TNF alpha ) plus interleukin 1 beta ( IL 1 beta ) stimulation was studied on cultured A 549 epithelial cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Oral administration of low dose KE , however , upregulated expression of IL 2 , IFN gamma and TNF alpha in the kidneys and suppressed glomerulonephritis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 12 dependent Th 1 differentiation was abrogated by LSF and yielded effector T cells that were deficient in proinflammatory cytokine secretion , including IFN gamma , IL 2 , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma primes macrophages for antimicrobial activity , increased killing of intracellular pathogens , and Ag processing and presentation to lymphocytes by cooperating with a second signal ( provided by LPS or endogenous TNF alpha ) to promote increased proinflammatory cytokine production , NO production , and MHC class 2 expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Protection against the mortality associated with disease models mediated by TNF and IFN gamma in mice lacking IFN regulatory factor 1 . ^^^ Mortality and cytokine production associated with disease models mediated by TNF and IFN gamma were studied in mice lacking IFN regulatory factor 1 ( IRF 1 ) . ^^^ KO mice showed lower circulating levels of TNF and IFN gamma than controls . ^^^ KO mice also showed lower TNF and IFN gamma mRNA in the spleen or liver than controls . ^^^ Mortality was also similar after the coinjection of two nonlethal doses of TNF and IFN gamma , a lethal combination ( LD 100 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The ability of the remaining spleen cells to produce IL 2 , IFN gamma , IL 4 and TNF alpha after in vitro specific stimulation decreased twofold , and the specific anti leishmanial antibodies declined 3 to 5 fold . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Clodronate LIP treatment prevented allograft rejection and strongly decreased the levels of IL 1beta , IL 1RA , IL 2 , IL 4 , IL 6 , IL 10 , IFN gamma , TNF beta / LT , MCP 1 , and MIP 2 mRNA in grafts and IL 1 beta , IL 2 , IL 4 , IL 6 , and IFN gamma mRNA in rims . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Amelioration of disease by rhIL 11 , as shown by reduced keratinocyte proliferation and cutaneous inflammation , was associated with decreased expression of products of disease related genes , including K 16 , iNOS , IFN gamma , IL 8 , IL 12 , TNF alpha , IL 1beta , and CD 8 , and with increased expression of endogenous IL 11 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , CCR 1 ( / ) mice showed enhanced Th 1 immune responses , including titers of antigen specific IgG2a antibody , delayed type hypersensitivity responses , and production of IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To investigate whether this impairment is related to a defect at the target cell binding and / or the postbinding level , we evaluated highly purified NK cells obtained from 125 C . trachomatis infected patients and compared them with 101 normal controls for their ability to kill K 562 and U 937 cell lines using a 51Cr release assay ; release tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) ; and kill anti IgM preincubated P 815 cell line ( ADCC activity ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokines investigated included the hematopoietic growth factors interleukin 3 ( IL 3 ) , IL 7 , and granulocyte macrophage colony stimulating factor ( GM CSF ) ; the lymphocyte regulatory cytokines IL 2 , IL 4 , and IL 10 ; the inflammatory regulators IL 1alpha and tumor necrosis factor alpha ( TNF alpha ) ; and the effector cell activating cytokines IL 5 and interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When immunized mice were challenged on day 28 after primary infection , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) were found to appear at 3 hr and to reach the maximum at 24 hr after challenge . ^^^ Spleen cells of mice primarily infected from 2 to 8 weeks before produced a significant amount of TNF alpha and IFN gamma when stimulated with formalin killed bacteria . ^^^ The administration of anti TNF alpha monoclonal antibody ( MAb ) and IFN gamma MAb increased bacterial proliferation in the organs of immune mice and exacerbated the secondary infection . ^^^ These results suggest that CD 4 , probably Th 1 T cells , play an important role for establishment of protective immunity against secondary C . pseudotuberculosis infection by secreting TNF alpha and IFN gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Confluent epithelial cell monolayer were pre incubated with cetirizine ( 0 . 01 1 . 0 micromol / L ) for 30 min and afterwards activated with pro inflammatory cytokines ( TNF alpha IL 1beta , IL 6 , IFN gamma ) or different agonists ( PMA , NaF , respiratory syncytial virus [ RSV ] ) for 24 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both the expression of activation markers ( CD 25 , CD 29 , CD 40 ligand , CD 69 and HLA DR ) on freshly isolated PBL and T cell subsets , and the expression of intracellular cytokines ( IL 4 , IL 10 , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) ) on mitogen stimulated PBL and T cell subsets were analysed by double immunofluorescent staining and flow cytometry . ^^^ Furthermore , in BD a significantly increased proportion of the gammadelta T cell population expressed CD 69 and high levels of CD 29 and were induced to produce IFN gamma and TNF alpha compared with healthy controls . ^^^ In contrast , an increased percentage of gammadelta T cells from RAS patients was induced to produce IFN gamma , but not TNF alpha . ^^^ These results indicate that in BD , activated gammadelta T cells , capable of producing IFN gamma and TNF alpha , are present in peripheral blood , suggesting that gammadelta T cells are dynamic and may be regulating immunopathogenic events . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
I . ) was determined by radioactive thymidine uptake while the production of Th 1 type cytokines ( interleukin 2 [ IL 2 ] , interferon gamma [ IFN gamma ] , and tumor necrosis factor alpha [ TNF alpha ] ) and Th 2 type cytokines ( IL 4 , IL 10 ) were measured by enzyme linked immunosorbent assay . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Hypothemycin also inhibited IL 6 , IL 10 , IFN gamma and TNF alpha production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , women with both virus infections ( HIV ( + ) / HPV ( + ) ) had significantly fewer IL 2 ( + ) CD 4 ( + ) , IFN gamma ( + ) CD 4 ( + ) , and TNF alpha ( + ) CD 4 ( + ) T cells than women with HPV infection alone ( HPV ( + ) ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
As stimulated CD 4 ( + ) P ( 2 ) T cells produce IFN gamma and TNF alpha , this could exacerbate blood nerve barrier breakdown that has been increasingly implicated in inflammatory demyelinating neuropathies ( IDNs ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Protection against lethal toxoplasmosis in mice by an avirulent strain of Toxoplasma gondii : stimulation of IFN gamma and TNF alpha response . ^^^ Mice infected with the PTN strain had an enhanced capacity to activate the immune system early in infection since they produced higher levels of IFN gamma , TNF alpha , and NO than PLK infected mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) alone can induce extracellular signal regulated kinase ( ERK ) , p 38 MAPK , and c Jun N terminal kinase activity in a rapid and transient manner , whereas interferon gamma ( IFN gamma ) can induce only ERK . ^^^ Salicylate treatment inhibited iNOS expression induced by TNF alpha and IFN gamma and attenuated the phosphorylation of ERK by TNF alpha and IFN gamma either alone or in combination . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , exposure of activated PBMC to the tumor cell supernatants resulted in an altered secretion of cytokines by these cells , i . e . the secretion of IFN gamma was generally reduced while the secretion of IL 1beta , IL 2 and TNF alpha was little affected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Diubiquitin expression was found to be generally and synergistically inducible with the cytokines IFN gamma and TNF alpha but not with IFN alpha . ^^^ The mouse homologue of diubiquitin which is also encoded in the MHC class 1 locus was likewise induced with IFN gamma and TNF alpha . ^^^ A general and synergistic induction with IFN gamma and TNF alpha suggests that diubiquitin may exert its functions in antigen presentation or other cellular processes controlled by these two cytokines . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The antitumor effect correlated well with a strong and rapid proinflammatory cytokine ( TNF alpha , IL 12 and IFN gamma ) response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Semiquantitative reverse transcription PCR analyses showed that both interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) mRNA levels in CD 4 ( + ) T cells became low when resistant C57BL / 6 mice were depleted of NK cells , suggesting that NK cells contribute to functional differentiation of CD 4 ( + ) T cells and thereby affect the induction of HSP 65 expression . ^^^ To determine the function of HSP 65 , macrophages were treated in vitro with antisense oligonucleotide for HSP 65 prior to inducing HSP 65 with IFN gamma plus TNF alpha or T . cruzi infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha and IL 8 expression was optimal in BEC pretreated with 50 IU of IFN gamma / ml , whereas TSST 1 stimulation of BEC pretreated with 200 IU of IFN gamma / ml failed to enhance either TNF alpha or IL 8 transcripts . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The present study was designed to measure the production of TNF alpha as well as additional cytokines , including interleukin 1beta ( IL 1beta ) , IL 6 , IL 8 , IL 12 , and gamma interferon ( IFN gamma ) but also to determine from what cells and at what time point during incubation with GBS that these cytokines are produced . ^^^ This suggests an important role for TNF alpha , IFN gamma , and IL 12 in GBS pathogenesis and / or immunity . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the mitogen stimulated whole blood cell cultures and in the urine samples the levels of the cytokines IL 1beta , IL 2 , IL 10 , TNF alpha and IFN gamma were determined by enzymoimmunological tests . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These CTL are H 2Kb restricted and produce IL 5 , IL 10 , IFN gamma , and small amounts of IL 4 , which is distinct from IFN gamma and TNF alpha that are typically secreted by virus specific CTL . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
T cells isolated from CD 28 deficient animals produced equivalent amounts of IFN gamma and TNF alpha in response to the immunogen , proteolipid protein 56 70 . ^^^ In fact , IFN gamma and TNF alpha production by Ag specific T cells was enhanced in both the B 7 1 and B 7 2 deficient NOD mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Ag specific IFN gamma and TNF alpha production by both primary and long term memory T cells was observed in < / =60 min after peptide stimulation . ^^^ Although the on rate kinetics of cytokine production were nearly identical , activated T cells produced more IFN gamma , but less TNF alpha , than memory T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Murine renal carcinoma ( Renca ) cells were constitutively resistant to Fas mediated killing in vitro , but exhibited increased expression of Fas and sensitivity to Fas mediated killing after exposure to IFN gamma and TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunization with MOG 35 55 peptide resulted in priming of both Th 1 ( lymphotoxin , IFN gamma , and TNF alpha ) and Th 2 ( IL 4 ) cells in the spleen . ^^^ CD 4 T cells that produced IFN gamma or TNF alpha were present in the CNS by day 7 after immunization with MOG 35 55 , peaked at day 20 , and then waned . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum levels of IFN gamma and TNF alpha were higher in B6 . lpr recipients than in control B 6 recipients , and monocytes / macrophages in B6 . lpr recipients appeared more sensitized . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study shows that subcutaneous administration of increasing doses of IL 12 , once a week , in 21 cancer patients increased the expression of cytokine genes ( interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) , IP 10 , MIG , IL 10 , IL 4 ) in peripheral blood mononuclear cells even at very low doses ( 30 ng / kg ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the presence of 10 ng / mL recombinant TNF alpha , the attenuation of LPS inducible IFN gamma release by G CSF was blunted in whole blood . ^^^ However , G CSF had no such effect on IFN gamma release from isolated lymphocytes stimulated with anti CD 3 or a combination of TNF alpha and IL 12 . ^^^ We conclude that the attenuation of IFN gamma release from lymphocytes is not a direct effect of G CSF on these cells but is rather due to the inhibition of monocytic IL 12 and TNF alpha release by G CSF . ( Blood . 2000 ; 95 : 270 276 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These enhancements were accompanied by an increase in cytokines [ interleukin ( IL ) 12 and tumour necrosis factor ( TNF ) alpha ] and RNI release by murine inflammatory macrophages primed with IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 12 produced by macrophages is an inducer of IFN gamma production , which in turn activates the macrophage antibacterial activity and synergizes its effects with TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The plasma concentrations of two antitumor cytokines , interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) , were greater in the food restricted group before tumor inoculation ( P < 0 . 05 ) . ^^^ Furthermore , the food restricted mice had significantly higher plasma levels of IFN gamma and TNF alpha after tumor inoculation , although the treatment significantly increased these cytokine levels in both groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Significant amounts of interferon gamma ( IFN gamma ) were detected in the PEC cultures on LPS stimulation , and anti IFN gamma antibody suppressed the LPS induced NO , but not TNF alpha and IL 6 , production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Conditioned media ( CM ) from PBMC stimulated in short term culture with Q fever antigens , PHA or measles antigen ( as an unrelated antigen ) were assayed for IL 2 , IL 4 , IL 5 , IL 6 , IL 10 and IFN gamma by AgEIA , and for IL 1 and TNF alpha / beta by bioassay . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using Caco 2 cells , we examined the effect of TNF alpha , IL 1 , IFN gamma , and TGF beta on E cadherin expression . ^^^ E cadherin expression in Caco 2 cells was significantly reduced after TNF alpha , IL 1 , and IFN gamma stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Compared with control tissues , we found elevated macrophage inflammatory protein 1alpha ( MIP 1alpha ) and interferon gamma ( IFN gamma ) expression , but not macrophage derived chemotactic factor ( MDC ) or TNF alpha , in tissues of patients with HPS irrespective of the cause or setting . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We speculated that cell mediated immunity would be important for recovery from and potentially in the clinical manifestations of HGE ; thus , serum tumor necrosis factor alpha ( TNF alpha ) , interleukin 1beta ( IL 1beta ) , gamma interferon ( IFN gamma ) , IL 10 , and IL 4 concentrations were studied . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor , interleukin 12 ( IL 12 ) , IL 6 , and interferon gamma ( IFN gamma ) appeared in the serum , and levels of nitrogen oxide ( NO ) derivatives were increased in serum and bronchoalveolar fluid . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , tumor necrosis factor alpha ( TNF alpha ) plus IL 1beta plus interferon gamma ( IFN gamma ) increased IL 8 in culture supernatant of epithelial cells ; the combination of NO synthase ( NOS ) inhibitors , aminoguanidine ( AG ) plus N ( G ) nitro L arginine methyl ester ( L NAME ) attenuated the cytokine induced IL 8 production in epithelial cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Compared with medium control , TNF beta significantly decreased IL 5 ( p = 0 . 0004 ) and IL 13 ( p = 0 . 008 ) but increased IFN gamma ( p = 0 . 001 ) production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Results favoring the T helper 1 ( Th 1 ) / T helper 2 ( Th 2 ) model during pregnancy were : significantly higher expression of interferon gamma ( IFN gamma ) in trophoblast samples from Group 3 versus 2 and in decidual tissue from Group 5 versus 4 ; stronger positivity of interleukin ( IL ) 10 in decidual tissue samples from Group 1 versus Groups 2 and 5 ; and higher expression levels of tumor necrosis factor beta ( TNF ) beta by the trophoblast in Group 5 versus 1 . ^^^ Against the Th1 / Th2 paradigm were the following findings : the significantly increased expression of IFN gamma by decidual or trophoblast samples in Groups 1 versus 2 , 2 versus 3 , and 1 versus 5 ; and the significantly higher expression of TNF alpha in decidual samples from patients in Group 1 ( but also Group 4 ) as compared with Group 5 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Peripheral nerve injury induces endoneurial expression of IFN gamma , IL 10 and TNF alpha mRNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 12 secretion by microglia is significantly reduced by TNF and IFN gamma antagonists showing that the IL 12 production is subject to regulation by auto and paracrine stimuli . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After 96 hours , culture supernatants were harvested to determine the concentrations of IgE , interleukin ( IL ) 5 , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) . ^^^ The levels of IgE , IL 5 , and IFN gamma , but not of TNF alpha , were inversely correlated with the duration ( in years ) of IT , and none of the levels of IgE ( P = . 74 ) , IL 5 ( P = . 15 ) , IFN gamma ( P = . 61 ) , and TNF alpha ( P = . 55 ) differed significantly between the nonatopic group and those who had been treated with IT for 10 years or more . ^^^ The levels of IL 5 were significantly lower in the good responders than in the poor responders to IT ( P < . 001 ) , whereas the levels of total IgE ( P = . 20 ) , IFN gamma ( P = . 16 ) , and TNF alpha ( P = . 14 ) did not differ significantly between them . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : BEAS 2B human bronchial epithelial cells were cultured with TNF alpha , IL 4 , IL 13 , and IFN gamma alone or in combination , after which supernatants were assayed for eotaxin , IL 8 , and RANTES proteins with ELISA . ^^^ RANTES production by TNF alpha was not affected by IL 4 and IL 13 but was markedly enhanced by IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : CD 34 ( + ) precursor cells were isolated from cord blood with use of high gradient magnetic cell sorting , cultured with GM CSF , TNF alpha , IL 4 , or IFN gamma , and surface and cytoplasmic staining for flow cytometry were performed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study examined the properties and responsiveness to cytokines of macrophages purified from normal and nephritic glomeruli to ascertain whether macrophages activated in vivo develop programmed unresponsiveness to cytokines as do bone marrow derived macrophages in vitro when activated by interferon gamma ( IFN gamma ) , tumor necrosis factor ( TNF ) , interleukin 4 ( IL 4 ) , or transforming growth factor beta ( TGF beta ) . ^^^ Macrophages from normal glomeruli did not generate nitric oxide ( NO ) spontaneously but only after treatment with IFN gamma and TNF alpha . ^^^ NO generation by these macrophages was abrogated by administering IL 4 , TGF beta , or TNF alpha before but not after IFN gamma treatment . ^^^ Glomerular macrophages also expressed beta glucuronidase , which was increased by TGF beta and decreased by IFN gamma and TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha , IL 1beta , and IFN gamma all inhibited CMV expression ( 70 + / 4 . 2 % , 65 + / 3 . 4 % , and 82 + / 3 . 6 % inhibition of viral expression , respectively , n = 5 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In immunostimulated macrophages and spleen cells , inosine potently inhibited the production of the proinflammatory cytokines TNF alpha , IL 1 , IL 12 , macrophage inflammatory protein 1alpha , and IFN gamma , but failed to alter the production of the anti inflammatory cytokine IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It has previously been shown that interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) together increase CD 95 surface expression on eosinophils . ^^^ We therefore investigated whether the increase in CD 95 expression mediated by IFN gamma / TNF alpha indeed translates into increased , FasL mediated apoptosis of eosinophils . ^^^ Prestimulation with IFN gamma / TNF alpha for 24 h significantly enhanced FasL induced apoptosis in eosinophils . ^^^ This increase in CD95 / FasL mediated apoptosis was correlated with an IFN gamma / TNF alpha mediated increase in CD 95 expression . ^^^ From these findings we conclude that the combination of IFN gamma and TNF alpha enhances CD 95 expression , which results in an increase in FasL mediated apoptosis of eosinophils in vitro . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
An overexpression of TNFalpha , IFN gamma , and IL 2 was also observed in PBMCs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Recent studies have focused particular attention on CD4+ T cells which produce excessive quantities either of Th 1 cytokines ( IFN gamma and TNF ) directed by IL 12 or of a Th 2 cytokine ( IL 4 ) , relative to the production of suppressive cytokines such as IL 10 and transforming growth factor beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To date , our results demonstrate that CD8+ T cell derived IFN gamma and TNF are not required effector functions . ^^^ To assess the potential for redundancy in effector mechanisms , we have generated mice deficient in both perforin and IFN gamma and are developing mice deficient in perforin and TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine ( IFN gamma und TNF alpha ) rather than cytotoxicity based effector functions are more critical for protective immunity both during the acute and chronic phases of T . gondii infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The activation of microglia and astrocytes by IFN gamma or a combination of this cytokine with TNF alpha appears to be an important effector mechanism in the host immunity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To investigate the role of pleiotropic neuronal and glial cytokines in the regulation of presenilin ( PS ) gene expression in human neural cells , both presenilin 1 ( PS 1 ) and presenilin 2 ( PS 2 ) mRNA levels were analysed by Northern blotting in SK N SH neuroblastoma , IMR 32 neuroblastoma , NTera 2 teratocarcinoma derived differentiated neurones ( NTera 2 N ) and U 373MG astrocytoma cells following exposure to proinflammatory cytokines ( TNF alpha , IFN gamma , or IL 1beta ) , anti inflammatory cytokines ( IL 10 or TGF beta 1 ) , dibutyryl cyclic AMP or phorbol 12 myristate 13 acetate ( PMA ) . ^^^ By contrast , PS 2 mRNA expression was upregulated substantially in SK N SH cells by exposure to TNF alpha and in U 373MG cells by treatment with IFN gamma , whereas it was downregulated in both NTera 2 N and U 373 MG cells following exposure to IL 1beta or PMA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Specific IFN gamma , IL 6 , and TNF alpha receptors have been found on human lymphocytes and other cell types . ^^^ PATIENTS AND METHODS : We assayed IFN gamma , TNF alpha , and IL 6 binding on peripheral blood T cells from MS patients , as compared with healthy subjects . ^^^ T cells from MS patients have significantly less IFN gamma receptors , and more TNF alpha and IL 6 receptors than those from controls . ^^^ RESULTS : T lymphocytes from untreated MS patients , which had significantly smaller amounts of IFN gamma receptors than those from controls , and more TNF alpha and IL 6 receptors than controls showed a significant increase in IFN gamma binding , and a significant decrease in TNF alpha and IL 6 binding after a 3 month IFN beta 1b treatment . ^^^ T cell IFN gamma Bmax values were even higher , and those of TNF alpha and IL 6 were lower after 6 months . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunization with oxidized M FP led to the secretion of T 1 cytokines from CD8+ T cells ( IL 2 , IFN gamma , and tumor necrosis factor alpha [ TNF alpha ] ) and from CD4+ T cells ( IL 2 and IFN gamma ) . ^^^ The simultaneous immunization of both oxidized M FP and FP led to the production of both T 1 and T 2 cytokines from CD8+ T cells ( IL 2 , IFN gamma , and TNF alpha ) and CD4+ cells ( IL 2 , IFN gamma , IL 4 , and IL 10 ) and IL 12 production in CD8+ cultures that is , both types of immune responses could occur together . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Flow cytometry was used to measure cell surface expression of platelet / endothelial cell adhesion molecule 1 ( PECAM 1 ) , intercellular adhesion molecule 1 ( ICAM 1 ) , vascular cell adhesion molecule 1 ( VCAM 1 ) , E selectin , major histocompatibility complex ( MHC ) class 1 and MHC class 2 , CD 40 , CD 95 ( fas ) and lymphocyte function associated antigen 3 ( LFA 3 ) before and after treatment with the cytokines tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) . ^^^ Significant differences were found in the ability to respond to cytokines between HUVEC and the cell lines , the greatest differences being induction of VCAM 1 and E selectin in response to TNF alpha and induction of MHC class 2 antigens in response to IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The alpha GalCer mediated abortion was not observed in Valpha 14 NKT , IFN gamma , tumor necrosis factor alpha , or perforin knock out mice and appeared to be due to the degeneration of embryonic trophoblasts mediated by the activated Valpha 14 NKT cells whose perforin dependent killing and production of IFN gamma and tumor necrosis factor alpha were essential . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Ex vivo flow cytometry determination of intracytoplasmic expression of IL 2 , IL 6 , IFN gamma , and TNF alpha in monocytes and T lymphocytes , in chronic hemodialysis patients . ^^^ AIMS : To determine the intracytoplasmic expression of TNF alpha , IL 2 , IL 6 and IFN gamma , ex vivo and in vitro , in both monocytes and T lymphocytes by flow cytometry after appropriate stimulation using phorbol myristate acetate ( PMA ) / ionomycin or lipopolysaccharides ( LPS ) in the presence of monensin , in order to assess the bio ( in ) compatibility of different dialysis membranes . ^^^ RESULTS : Before any stimulation there was a statistically significant difference in the percentages of TNF alpha , IL 6 , and IFN gamma expressing monocytes with respect to the dialysis membrane used . ^^^ One hour after LPS stimulation the patterns remained unchanged for TNF alpha and IFN gamma , whereas the percentages of IL 6 expressing cells in PS patients and in healthy volunteers reached the figures obtained in the other groups . ^^^ When we examined the percentage of IFN gamma , TNF alpha and IL 6 expressing monocytes in patients before and after a dialysis session , before any stimulation , we found that the results were significantly different for the three membranes ( p = 0 . 01 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma abolished the enhancement of MMP 13 and MMP 1 expression by transforming growth factor beta ( TGF beta ) and tumor necrosis factor alpha ( TNF alpha ) , and inhibited invasion of A 5 cells through type 1 collagen . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , iNOS and NO generation could be induced in vitro by inflammatory cytokines ( mixture of interleukin 1beta , IFN gamma , and tumor necrosis factor alpha ) in three human cholangiocarcinoma cell lines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
By using reverse transcription polymerase chain reaction ( RT PCR ) analysis , it was shown that immune lymph node cells expressed message for IFN gamma , tumour necrosis factor ( TNF ) alpha and interleukin ( IL ) 10 after stimulation with heat killed S . schenckii . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Fas is constitutively expressed by primary murine microglia at a low level and significantly up regulated by TNF alpha or IFN gamma stimulation . ^^^ In microglia , Fas expression is regulated at the level of mRNA expression ; TNF alpha and IFN gamma induced Fas mRNA by approximately 20 fold . ^^^ STAT 1alpha and NF kappaB activation are involved in IFN gamma or TNF alpha mediated Fas up regulation in microglia , respectively . ^^^ Upon incubation of microglial cells with FasL expressing cells , approximately 20 % of cells underwent Fas mediated cell death , which increased to approximately 60 % when cells were pretreated with either TNF alpha or IFN gamma . ^^^ TGF beta treatment inhibited Fas mediated cell death of TNF alpha or IFN gamma stimulated microglial cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both proteins inhibited spleen cell proliferation and IFN gamma secretion in response to stimulation with type 2 collagen , but only vIL 10 reduced the synovial mRNA levels of the proinflammatory cytokines IL 1beta , TNF alpha , and IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CD 163 expression is also suppressed by proinflammatory mediators like lipopolysaccharide ( LPS ) , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha , whereas IL 6 and the antiinflammatory cytokine interleukin 10 ( IL 10 ) strongly up regulate CD 163 mRNA in monocytes and macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Acute infection was characterized by a predominantly T helper ( Th ) 1 ( IL 2 and IFN gamma ) and proinflammatory ( TNF alpha and MIP 1beta ) type of cytokine response and the absence of a Th 2 type of response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , PKR was required to establish a sustained response to tumor necrosis factor alpha ( TNF alpha ) and to potentiate activation of NF kappaB by cotreatment with TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After oral or intragastric administration of BCG to mice , comparable numbers of IFN gamma and TNF gamma producing cells were detected in both local ( Peyer ' s patches ) and central ( spleen ) lymphoid organs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , these components synergistically induce secretion of tumor necrosis factor alpha ( TNF alpha ) , which also synergizes strongly with IFN gamma . ^^^ IFN gamma activated signal transducer and activator of transcription 1 and interferon regulatory factor 1 function synergistically with nuclear factor kappaB activated by LPS and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of various cytokines , such as interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) , and FasL gene expression in the bone marrow increased concomitantly . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lipopolysaccharide ( LPS ) or interferon gamma ( IFN gamma ) stimulated microglia or BV 2 cells exhibit properties of activated microglia such as high levels of TNFalpha and IL 6 release . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In xeno MLC , levels ( pg / ml ) of tumor necrosis factor alpha ( TNF alpha ) ( 163+ / 17 ) , interferon gamma ( IFN gamma ) ( 278+ / 60 ) , IL 5 ( 24+ / 10 ) , IL 6 ( 2959+ / 923 ) , and IL 10 ( 17+ / 2 ) were produced in greater amounts than autologous controls ( P < 0 . 05 ) . ^^^ The levels of TNF alpha , IFN gamma , IL 6 , and IL 10 but not IL 5 were significantly ( P < 0 . 05 ) lower in xeno MLC than those produced in allo MLC . ^^^ All of these cytokines were also produced in MILC when human peripheral blood mononuclear cells ( PBMC ) were cocultured with ICCs , levels ( pg / ml ) being TNF alpha ( 308+ / 47 ) , IFN gamma ( 93+ / 17 ) , IL 5 ( 6 . 2+ / 3 ) , IL 6 ( 5649+ / 421 ) , and IL 10 ( 122+ / 18 ) . ^^^ Addition of IL 10 significantly inhibited the production of TNF alpha , IFN gamma , IL 5 , and IL 6 by 76 , 96 , 100 , and 93 % , respectively , in xeno MLC . ^^^ Addition of IL 10 also significantly ( P < 0 . 05 ) inhibited the production of TNF alpha , IFN gamma , IL 5 , and IL 6 by 88 , 91 , 100 , and 96 % , respectively , in MILC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
High serum levels of tumor necrosis factor alpha ( TNF alpha ) , interleukin 2 ( IL 2 ) , IL 4 , and interferon gamma ( IFN gamma ) were found in the serum after the first 2 injections of Con A but rapidly decreased from the third injection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , Con A injection leads to a marked increase in plasma tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) levels . ^^^ In addition , galectin 1 almost completely prevented the Con A induced increase in plasma TNF alpha and IFN gamma , an effect that was , at least in part , independent on the elimination of activated T helper cells , because galectin 1 prevented lipopolysaccharide ( LPS ) induced release of TNF alpha and IFN gamma also from macrophages in vitro , without affecting their viability . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Detectable levels of tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) but not of interleukin 2 ( IL 2 ) or IL 4 were easily observed in NK cells . ^^^ Other known stimuli for NK cells ( IL 2 and CD 16 monoclonal antibody and incubation with K 562 , the NK sensitive cell line ) promoted IFN gamma and TNF alpha production in NK cells to a lesser extent than did PMA and ionomycin stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine messages for pulmonary tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) , as well as for the lytic molecules perforin and granzyme B , were quantified . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To investigate the relationship between TNF alpha and IL 12 production , we analyzed the effects of exposure of human monocyte derived macrophages to TNF alpha on LPS or Staphylococcus aureus induced IL 12 production in the presence or absence of IFN gamma . ^^^ The major transcriptional factors identified to be involved in the regulation of IL 12 p 40 gene expression by LPS and IFN gamma , i . e . , c Rel , NF kappaB p 50 and p 65 , IFN regulatory factor 1 , and ets 2 , were not affected by TNF alpha when examined by nuclear translocation and DNA binding . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We examined the possibilities that IL 4 stimulation of IFN gamma production , suppression of IL 1 or IL 2 production , or induction of TNF alpha or Fas mediated apoptosis could account for IL 4 ' s suppressive effect . ^^^ The suppressive effect of IL 4 was not reversed by IL 1 , IL 2 , or anti TNF alpha or anti IFN gamma mAb treatment , or mimicked by treatment with anti IL 2Ralpha ( CD 25 ) and anti IL 2Rbeta ( CD 122 ) mAbs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Monocyte chemoattractant protein 1 ( MCP 1 ) is a chemokine that is expressed by a variety of tissue cells in response to inflammatory stimuli , such as IL 1beta , TNF alpha , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Rat bone marrow derived macrophages primed with IFN gamma then further activated in coculture with LPS or TNF alpha elicited a 10 fold induction of rat mesangial cell apoptosis and complete suppression of mitosis , effects inhibitable by the NO synthase inhibitors L monomethyl arginine and L N ( 6 ) ( 1 iminoethyl ) lysine dihydrochloride . ^^^ Nevertheless , when mesangial cells were primed with IFN gamma plus TNF alpha , increased induction by activated macrophages of mesangial apoptosis exhibited a NO independent element . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Previously , we showed that depletion of gamma delta T cells significantly reduced clinical and pathological signs of disease , which was associated with reduced expression of IL 1 beta , IL 6 , TNF alpha , and lymphotoxin at disease onset and a more persistent reduction in IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The aqueous extract of D . febrifuga roots ( AEDF ) inhibited the secretion of NO and TNF alpha in lipopolysaccharide ( LPS ) and / or interferon gamma ( IFN gamma ) stimulated mouse peritoneal macrophages , without affecting cell viability . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The keratinocyte line HEK 001 expressed ICAM 1 following stimulation with TNF alpha or IFN gamma and following coculture with autologous cutaneous T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the presence of IFN gamma , stimulation of macrophages with TNF alpha results in macrophage differentiation along a pathway in which iNOS is expressed , whereas , in the absence of IFN gamma , stimulation of macrophages with TNF alpha results in differentiation along a pathway in which IGF 1 is expressed . ^^^ The authors focus on some of the molecular events involved in TNF alpha and IFN gamma signal transduction and the regulation of iNOS and IGF 1 genes in macrophages . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Recently emerging evidence suggests that macrophages are able to release not only IL 12 and TNF alpha but also IFN gamma . ^^^ While macrophages release TNF alpha in an indiscriminate and IL 12 independent way , the release of IL 12 , particularly bioactive IL 12 p 70 , and IFN gamma is under tight control . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Administration of TJ 41 decreased the BALF concentrations of IL 1alpha , IL 6 and GM CSF , but not TNF alpha or interferon gamma ( IFN gamma ) , on day 4 after infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Unstimulated ECs expressed relatively low levels of Fas , but expression was upregulated after treatment with tumor necrosis factor alpha ( TNF alpha ) or interferon gamma ( IFN gamma ) . ^^^ In contrast , VSMCs expressed relatively high levels of Fas , and treatment with TNF alpha or IFN gamma induced little or no upregulation under the conditions of these assays . ^^^ IFN gamma enhanced Adeno FasL induced death of VSMCs , but TNF alpha did not . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OBJECTIVE : The purpose of this study is to determine the effects of alpha chemokines ( IL 8 and melanoma growth stimulating activator ) , beta chemokines ( monocyte chemotactic protein 1 , macrophage inflammatory protein 1alpha [ MIP 1alpha ] , MIP 1beta , and RANTES ) , and proinflammatory cytokines ( IL 1beta , TNF alpha , and IFN gamma ) on the expression of both ET 1 and endothelin converting enzyme 1 ( ECE 1 ) by human umbilical vein endothelial cells . ^^^ METHODS : Subconfluent monolayers of human umbilical vein endothelial cells were incubated with each chemokine individually for 24 hours or with a mixture ( cytomix ) of TNF alpha , IL 1beta , and IFN gamma for 6 and 24 hours . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Suppression of EAN was associated with down regulated myelin antigen induced T cell responses as well as down regulated IFN gamma and TNF alpha production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma priming augmented Gal lectin induced IL 12 mRNA expression independent of TNF alpha and IL 1beta , and was required for IL 12 p 70 protein production from macrophages and human peripheral blood mononuclear cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Physiological stimulators , TNF and interferon gamma ( IFN gamma ) did not cause differential promoter activity between TNF 1 and TNF 2 , but LPS did with only the TNF 2 promoter / 3 ' UTR construct being significantly responsive to lipopolysaccharide ( LPS ) in U 937 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There were no significant changes in the gene transcript levels of interleukin 1B ( IL 1beta ) , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and granulocyte macrophage colony stimulating factor ( GM CSF ) either in the bronchial tissue or BW cells after DE exposure at this time point . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , inflamed hearts from IL 4 / mice displayed higher levels of IFN gamma and TNF alpha transcripts as compared to IL 4 + / + mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Budesonide and formoterol were added in the absence or presence of interleukin ( IL ) 1beta , tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) or IL 4 to human lung fibroblasts ; ICAM 1 and VCAM 1 expression were measured after 8 h using a cell surface enzyme linked immunosorbent assay ( ELISA ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
For L pigs , IL 1alpha , IL 8 , IL 10 , and TNF alpha in SM correlated with CFU , while IL 6 , TNF beta , and IFN gamma in SF correlated with CFU . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Decreased resistance of B cell deficient mice to infection with Toxoplasma gondii despite unimpaired expression of IFN gamma , TNF alpha , and inducible nitric oxide synthase . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition to stimulating IFN gamma synthesis , IL 18 also possesses inflammatory effects by inducing synthesis of the proinflammatory cytokines TNF and IL 1beta and the chemokines IL 8 and macrophage inflammatory protein 1alpha . ^^^ The increased survival was accompanied by decreased levels of IFN gamma and macrophage inflammatory protein 2 in anti IL 18 treated animals challenged with E . coli LPS , whereas IFN gamma and TNF concentrations were decreased in treated mice challenged with S . typhimurium . ^^^ This protection is partly mediated through inhibition of IFN gamma production , but mechanisms involving decreased neutrophil mediated tissue damage due to the reduction of either chemokines ( E . coli LPS ) or TNF ( S . typhimurium LPS ) synthesis by anti IL 18 treatment may also be involved . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In short , ch RecHIGH POS CTLs are triggered by TAAHIGH POS as well as TAALOW POS RCCs to lyse tumor cells and produce ( IFN gamma and TNF alpha ) lymphokine . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The disease modulating effects of exogenous IL 10 and either genetical or pharmacologically induced IL 10 deficiency were associated with profound and opposite modifications of the Con A induced increase in the circulating levels of IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , neutralization of either IL 18 or TNF alpha did not inhibit Con A induced production of IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , administration of IFN gamma did not induce the production of both cytokines in infected mice , although tumor necrosis factor ( TNF ) alpha and IFN gamma inducible protein ( IP ) 10 were synthesized by the same treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine induced levels of ICAM 1 expressed by rat brain endothelial cells were quantitated by enzyme immunoassay in response to stimulation by TNF alpha in the presence or absence of IFN gamma . ^^^ Furthermore , evidence for a synergistic interaction of both TNF alpha and IFN gamma was observed in the BN strain . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine analysis by competitive PCR revealed that IL 10 mRNA in the lymphoid organ was significantly suppressed in the PT ( + ) group , whereas levels of IFN gamma , TNF alpha and TGF beta mRNA were insignificantly different after PT administration . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Thus , we have investigated effects of IL 3 , IL 5 , GM CSF , IL 8 , RANTES and the proinflammatory cytokines TNF alpha and IFN gamma , on superoxide anion and NO generation by clone 15 HL 60 human eosinophilic cells . ^^^ In the presence of L NAME , PMA ( 1 nM ) stimulation significantly increased superoxide anion generation following 3 h treatments with IL 3 , TNF alpha or IFN gamma . ^^^ Eighteen hour cytokine treatments with GM CSF , IL 8 , RANTES , IFN gamma or TNF alpha primed the cells for enhanced reactive oxygen species following exposure to an EOS stimulant . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Clinical and laboratory variables , and circulating levels of interleukin 2 ( IL 2 ) , IL 4 , IL 10 , IL 12 , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) measured by ELISA were compared between patients ( cyclosporine group ) treated with cyclosporine plus MTX and those ( placebo group ) treated with placebo plus MTX at entry and at 16 weeks . ^^^ Comparison of circulating cytokines at entry and at 16 weeks showed significant decreases of IL 2 ( median 61 vs 7 pg / ml ; p = 0 . 004 ) ( `` + ' ' denotes increase , `` ' ' denotes decrease ) , IL 12 ( median 313 vs 14 pg / ml ; p = 0 . 002 ) , TNF alpha ( median 55 vs 5 pg / ml ; p < 0 . 001 ) , and IFN gamma ( median 21 vs 5 pg / ml ; p = 0 . 003 ) , and a significant increase of IL 10 ( median 55 vs 12 pg / ml ; p < 0 . 001 ) in the cyclosporine group compared with the placebo group . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Among the children with early , untreated JRA , a median of 3 to 8 % of inflammatory cells in synovial samples expressed mRNA for the proinflammatory cytokines interleukin 1beta ( IL 1beta ) , tumor necrosis factor alpha ( TNF alpha ) , or interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Significantly reduced production of IFN gamma , IL 4 and IL 12 and significantly increased production of IL 6 , IL 10 and TNF alpha were found in patients with SLE . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , IFN gamma did not affect TNF alpha plasma levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Analysis of cytokine expression demonstrated that the MCMV induced gammadelta T cells expressed interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) but not interleukin 4 ( IL 4 ) , implying their participation in the cell mediated immune response against MCMV . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cellular contents of the chamber exudates were used for differential cell counts , and the supernatants were analysed for tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and interleukin ( IL ) 10 levels . ^^^ The results showed that a repeat local challenge with P . gingivalis augmented the proinflammatory cytokines TNF alpha and IFN gamma , while inhibiting the accumulation of the anti inflammatory cytokine IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of modulators of endometrial function , including lipopolysaccharide ( LPS ) , tumour necrosis factor ( TNF ) alpha , interleukin ( IL ) 1beta , IL 4 and interferon gamma ( IFN gamma ) , on the production of RANTES by endometrial stromal cells ( ESC ) were examined by an enzyme linked immunosorbent assay and Northern blot analysis . ^^^ IFN gamma synergistically enhanced the TNF alpha and LPS induced RANTES expression , but had no effect on the IL 1beta induced RANTES expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Other exciting new methods to treat IBD could be use of monoclonal antibodies to effector T cell molecules ( such as CD 4 or CD44v7 ) removal of such cytokine secreting cells ( apheresis ) , antibodies to proinflammatory cytokines ( such as TNF alpha , IFN alpha , IFN gamma , and IL 12 ) or administration of anti inflammatory cytokines ( such as IL 10 , IL 11 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 2 , IL 4 , IL 5 , IL 10 , IL 12p40 , IFN gamma , TNF alpha , and TGF beta 1 mRNA expression was determined by semiquantitative reverse transcriptase polymerase chain reaction . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To investigate the effects of pro inflammatory cytokines such as interleukin 1beta ( IL 1beta ) , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) on apoE secretion by CCF STTG 1 cells , a sensitive and specific double sandwich Enzyme Linked ImmunoSorbent Assay ( ELISA ) was developed . ^^^ After 48 h of incubation , apoE secretion was inhibited by TNF alpha but not affected by IL 1beta and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Significantly less OK PSA encapsulated into liposomes ( Lipo OK PSA ) than OK PSA alone ( 1 / 100 or less of OK PSA alone ) was required to induce IFN gamma , tumor necrosis factor alpha ( TNF alpha ) , TNF beta , interleukin 1 beta ( IL 1 beta ) , natural killer , and lymphokine activated killer activities by human peripheral blood mononuclear cells and mouse spleen cells . ^^^ Furthermore , high levels of natural killer and lymphokine activated killer activities and a significant increase in the number of cells positive for asialo GM 1 , IFN gamma , TNF alpha , or IL 1 beta were detected in the spleen cells derived from the animals given Lipo OK PSA compared with those given saline . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our results indicated that IL 2 given subcutaneously at a low dose of 100 microg / day for 3 weeks induced IFN gamma and TNF alpha in plasma ( measured 24 hrs after the last dose of IL 2 ) and affected the ability of blood leukocytes to produce cytokines . ^^^ Production of IFN gamma induced in vitro with PHA was enhanced , but TNF alpha production induced by lipopolysaccharide ( LPS ) and virus ( Newcastle Disease Virus ) was depressed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum interleukin 10 ( IL 10 ) levels measured significantly higher in tolerized mice than in controls , whereas interferon gamma ( IFN gamma ) , IL 2 , and tumor necrosis factor alpha ( TNF alpha ) were reduced significantly . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha , IL 4 , and IFN gamma regulate differential expression of P and E selectin expression by porcine aortic endothelial cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation of endothelial cells with tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) is associated with 20 80 % reduction of extracellular phosphorylation of all membrane proteins . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In rheumatoid arthritis a potentially protective role is suggested by the local synthesis of both FHL 1 / reconectin and factor H in synovial fibroblasts and their induction by the anti inflammatory agent dexamethasone and the cytokine IFN gamma , but not by TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Generally , the treatment of macrophages with lead results in disregulation of the production of proinflammatory cytokines [ tumour necrosis factor alpha ( TNF alpha ) , interleukin 1alpha ( IL 1alpha ) and interleukin 6 ( IL 6 ) ] and preferential production of Th 1 type of cytokines ( IFN gamma and IL 2 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Small amounts of lipopolysaccharide ( LPS ) or tumor necrosis factor alpha ( TNF alpha ) synergized with IFN gamma in the production of NO from irradiated BNL CL . 2 cells , even though LPS or TNF alpha alone did not induce NO production from the same cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Accordingly , we sought to determine in this study whether zinc ion may have effect on IFN gamma or TNF alpha induced NO production and iNOS expression in cultured murine keratinocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma , TNF alpha and IL 10 have been shown to be involved in the regulation of LPS induced serum levels of the NO oxidation products nitrate and nitrite . ^^^ Therefore , in the present study , we investigated the role of endogenous IFN gamma , TNF alpha and IL 10 in the regulation of LPS induced tissue iNOS expression in the major organs . ^^^ To this end , mice were pre treated with anti IFN gamma , anti TNF alpha , anti IL 10 monoclonal antibodies , or combinations of these , two hours before intraperitoneal LPS challenge . ^^^ Immunohistochemical staining for iNOS and determination of iNOS activity indicated that iNOS expression was mainly upregulated in the small intestine , lung and heart , and that IFN gamma , TNF alpha as well as IL 10 are involved in the regulation of iNOS expression and enzyme activity . ^^^ Whereas blocking either IFN gamma or TNF alpha did not affect iNOS expression , iNOS enzymatic activity seems to be inhibited . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cultured I / CB PE cells expressed mRNA for TGF beta 1 , TGF beta 2 , IL 6 , IL 10 , and TNF alpha , but not IL 4 , IFN gamma , proopiomelanocortin ( POMC ) , and CD95L ( Fas L ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The mRNA expression and release of different cytokines including interleukin ( IL ) 1beta , IL 2 , IL 4 , IL 6 , IL 8 , IL 10 , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) were measured . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we examined the adhesion and migration profiles of two human papillomavirus ( HPV ) DNA transfected keratinocyte derived cell lines , EIL 8 and 18 11S3 , and of the cervix adenocarcinoma SiHa cell line , towards laminin 1 , and the selective effect of a 24 72 h treatment of 1000 U / ml interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) , a treatment that significantly decreases cervix carcinoma cell proliferation and progression in nude mice , on these parameters . ^^^ Interestingly , treatment of the cells with the cytokines ( 1000 U / ml IFN gamma and TNF alpha ) did not modulate the adhesion properties of the cells , but chemotactic migration of SiHa cells to laminin 1 was significantly decreased , while migration towards type 1 collagen was increased . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neutralization experiments identified critical roles in this process for proinflammatory cytokines such as TNF alpha , IL 1beta , and IFN gamma as well as membrane molecules such as LFA 1 , CD 40 ligand , and membrane bound TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neither the pancreata of normal BALB / c mice nor NOD mice at 2 16 weeks of age contained tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , IL 4 , or IL 12 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Sections from the pancreata of these injected mice were stained for cytokines ( tumor necrosis factor alpha [ TNF alpha ] , interferon gamma [ IFN gamma ] , CD1d , interleukin 2 [ IL 2 ] , IL 4 , IL 6 , IL 10 , and IL 12 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
By 24 h p . i . a high titer serum viremia had seeded infectious virus systemically , coincident with the systemic induction of the proinflammatory cytokines interleukin 12 ( IL 12 ) p 40 , IFN gamma , tumor necrosis factor alpha , and IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma , TNF alpha , IL 10 and IL 12 ( ELISA ) . ^^^ In similar experiments , a significant increase in the cytolytic activity of HPNK cells was elicited by S . typhi Ty 2 but not by mutant strain MEI 028 ; neither of the cytokines assayed ( IFN gamma and TNF alpha ) was detected in the supernatant . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Compared with TBI and non BMT C3H / HeJ mice , SEB challenged chimeric mice at 6 8 weeks had ( 1 ) increased splenocyte mRNA expression for : IFN gamma ( 3 . 5 10 optimally at 1 h ) , TNF alpha ( 6 . 5 10 at 2 h ) , IL 6 ( 4 . 8 10 at 4 h ) , IL 1beta ( 8 . 4 10 at 4 h ) , IL 2 ( 4 . 7 10 at 4 h ) , and IL 10 ( 3 10 at 16 h ) , and ( 2 ) increased and earlier peak serum levels of IFN gamma , IL 6 , IL 1beta and IL 2 , but no increase in serum TNF alpha or IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokines TNF alpha and IFN gamma are shown to be important mediators of cellular damage in graft versus host reactions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Similarly , increased amounts of IL 2 , TNF alpha and IFN gamma were in the serum of patients compared with the control group in which these cytokines are barely detectable . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The present study was designed to characterize the effects of glucoprivation induced by 2 DG administration on concentrations of tumor necrosis factor alpha ( TNF alpha ) , interleukin 1beta ( IL 1beta ) , and IL 6 in the blood and interferon gamma ( IFN gamma ) , IL 2 , and IL 4 in vitro production by partially purified T splenocytes in BDF 1 mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : In vitro validation studies showed that the filter paper technique could be used to measure mucosal release of interleukin 1beta ( IL 1beta ) , tumour necrosis factor alpha ( TNF alpha ) , thromboxane B ( 2 ) ( TXB ( 2 ) ) , and prostaglandin E ( 2 ) ( PGE ( 2 ) ) , but not interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Similarly , IL 6 and IFN gamma potently enhanced IL 1beta or TNF alpha induced C 3 and factor B secretion , respectively . ^^^ In this study , we demonstrated C 3 and factor B secretion in A 549 cells , and showed that the proinflammatory cytokines , IL 1beta , IL 6 , TNF alpha and IFN gamma , acted as potent inducers of C 3 and factor B secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
During the acute phase of the infection the presence of IFN gamma , TNF alpha , IL 12 and GM CSF has been closely associated with resistance , whereas TGF ss and IL 10 have been associated with susceptibility . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
HCMV stimulated CD8+ T cells produced IFN gamma ( 1 . 11 % ) and TNF alpha ( 1 . 71 % ) ; VZV and HSV specific CD8+ T cells were not detectable . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
For example we have found that TNF alpha enhances IFN gamma induced IDO activity and antimicrobial effect in human glioblastoma cells whereas both IFN gamma mediated effects were blocked by TNF alpha as well as by IL 1 in a human uroepithelial cell line . ^^^ We were able to show that the IL 1 and TNF alpha mediated inhibition of IFN gamma induced IDO activity in uroepithelial cells is due to stimulation of inducible nitric oxide synthase . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The febrile responses were in parallel with the levels of interleukin 1 ( IL 1 ) , tumor necrosis factor ( TNF ) , interferon gamma ( IFN gamma ) , IL 2 , and IL 6 in supernatant fluids obtained from PBMC treated with SEA . ^^^ Both the pyrogenicity and the levels of IL 1 , TNF , IFN gamma , IL 2 , and IL 6 in supernatant fluids started to rise at 6 to 18 h and reached their peak levels at 24 to 96 h after SEA incubation . ^^^ Both the fever and the increased levels of IL 1 , TNF , IFN gamma , IL 2 , and IL 6 in supernatant fluids obtained from the SEA stimulated PBMC were decreased by incubating SEA PBMC with anisomycin ( a protein synthesis inhibitor ) , aminoguanidine ( an inhibitor of inducible nitric oxide synthase [ NOS ] ) , or dexamethasone ( an inhibitor of NOS ) . ^^^ The febrile response to supernatant fluids obtained from the SEA stimulated PBMC was attenuated by adding either anti IL 1beta , anti TNF alpha , or anti IFN gamma monoclonal antibody ( MAb ) to supernatant fluids . ^^^ The antipyretic effects exerted by anti IL 1beta MAb were greater than those exerted by anti TNF alpha or anti IFN gamma MAb . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Production of cytokines including gamma interferon ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) is an important early stage host response following infection with intracellular pathogens . ^^^ The levels of mRNA for IFN gamma , TNF alpha , IL 1beta , IL 6 , IL 10 , and IL 12 increased in both BALB / c and C57BL / 6 mice 24 to 36 h after infection . ^^^ Considering the role of TNF alpha and IFN gamma in the processes of endotoxic shock , these results also indicate that selected cytokines , while important for resistance to B . pseudomallei infection , are also potential contributors to immunopathology and the development of acute fulminating disease . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
BALB / c and strain 129 mice infected intranasally with Chlamydia pneumoniae displayed a moderate to severe inflammation in the lungs and produced interleukin 12 ( IL 12 ) , gamma interferon ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and IL 10 , with peak levels on days 1 to 3 postinfection ( p . i . ) , returning to basal levels by day 16 p . i . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We studied the expression of tumour necrosis factor ( TNF ) and interferon gamma ( IFN gamma ) . ^^^ The complex of IL 6+sIL 6R and Hyper IL 6 inhibited significantly the production of TNF in a gp 130 dependent manner , whereas no differences in IFN gamma expression were found . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , lamina propria T cells ( LP T ) from IBD patients were more responsive to IL 15 as compared with controls , and IL 15 alone without a primary T cell stimulus induced IFN gamma and TNF production by isolated IBD LP T cells , especially by LP T cells from patients with Crohn ' s disease . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The role of perforin , IFN gamma , and TNF alpha in anti tumor CD 8 T cell immunity was examined in a new tumor model using a CD 8 T cell epitope ( GP 33 ) derived from lymphocytic choriomeningitis virus as a tumor associated Ag . ^^^ Tumor regression was dependent on perforin , IFN gamma , or TNF alpha , because A9GP33 tumors developed in mice deficient in one of these genes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cell culture supernatants were analyzed by ELISA for IL 2 , IFN gamma , IL 4 , IL 10 , TNF alpha , IL 1 beta , and IL 12p40 . ^^^ T cell secretion of IL 2 , TNF alpha , and in part IFN gamma ( but not IL 4 ) was significantly diminished in non survivors throughout the entire course of sepsis , compared to controls and sepsis survivors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : In comparison with matched peripheral blood mononuclear cells , sinusoidal leucocytes produced more mRNA for IL 10 , IL 15 , TNF alpha , IL 18 , IFN gamma , FasL , perforin and granzyme . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The purpose of this study is to determine whether inflammatory mediators such as LPS , IFN gamma , and TNF alpha regulate RON expression . ^^^ TNF alpha plus IFN gamma abrogated macrophage RON expression , although individual cytokines had no significant effect . ^^^ Because LPS and TNF alpha plus IFN gamma induce NO production , we reasoned that NO might be involved in the RON inhibition . ^^^ LPS and TNF alpha plus IFN gamma are capable of down regulating RON expression through induction of NO production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Such activated CD8+ T cells produced IL 2 , IFN gamma , TNF alpha , and GM CSF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Analysis of intracellular cytokine expression showed that the striking majority of E 7 pulsed DC activated CD8+ T cells strongly expressed IFN gamma , TNF alpha and IL 2 but not IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The increase of NO synthesis by IFN gamma plus hCG was associated with the increase of tumor necrosis factor alpha ( TNF alpha ) secretion and hCG induced NO production was decreased by the treatment with anti murine TNF alpha neutralizing antibody . ^^^ This study provides evidence that hCG activates expression of iNOS protein in murine microglial cells accompanied by NO accumulation via pathway dependent on L arginine in the culture medium , and further offers that TNF alpha acts on the NO synthesis from IFN gamma primed murine microglial cells . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The immune response induced by r S . typhimurium p 30 was accompanied by augmented interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) levels produced by restimulated splenocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mediation of the immunomodulatory effect of beta estradiol on inflammatory responses by inhibition of recruitment and activation of inflammatory cells and their gene expression of TNF alpha and IFN gamma . ^^^ CONCLUSION : Collectively , the results suggest that E 2 mediated inhibition of inflammatory responses may be due to a combination of suppression of homing and activation of inflammatory cells and their production of TNF alpha and IFN gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha and IFN gamma have an important role in the generation of hsp 65 specific cytotoxic T lymphocytes ( CTL ) that lyse hsp 65 pulsed autologous macrophages ( hsp 65 CTL ) . ^^^ Since a positive feedback mechanism between TNF alpha , IFN gamma and IL 12 has been described , we undertook to evaluate the role of IL 12 on the hsp 65 CTL generation in leprosy patients . ^^^ Our results show that the presence of IL 12 during the first 24 h of the in vitro antigen stimulation amplifies the hsp 65 cytotoxic response whenever both IFN gamma and TNF alpha are present . ^^^ Anti IL 4 upregulated the binding of IFN gamma and did not modify that of TNF alpha so the low CTL activity could be as a result of IL 4 by a decrease of the IFN gamma binding on MB cells . ^^^ Cells from those MB patients taking thalidomide ( MB T ) did neither bind IFN gamma nor TNF alpha even when antigen or anti IL 4 were added , demonstrating that thalidomide inhibits either the in vitro binding or receptor expression of both TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In most cases , healthy household contacts of newly diagnosed multibacillary leprosy patients , designated exposed household contacts ( EC ) , showed low to undetectable in vitro IFN gamma production in addition to substantial TNF alpha production in response to M . leprae . ^^^ Post treatment lepromatous leprosy patients secreted relatively high levels of IL 10 in response to M . leprae , whereas one self cured tuberculoid leprosy patient produced simultaneously high levels of IFN gamma and TNF alpha . ^^^ Vaccination induced amplification of IFN gamma production with a concomitant decrease in TNF alpha / IL 10 ratios that resembled the cytokine pattern observed in R contacts . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Despite differences observed in the time course of cytokine increments and in serum cytokine levels , both venoms induced prominent elevations of TNF alpha , IL 1 , IL 6 , IL 10 and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Blood was stimulated with LPS for 24 h and supernatants were assayed for TNF alpha , IFN gamma and IL 10 by enzyme linked immunosorbent assay . ^^^ LPS induced production of TNF alpha and of IFN gamma was reduced in patients with colorectal carcinoma compared to controls ( TNF alpha , 11 , 269 pg / ml ( 1 ) 12 , 598 ; IFN gamma , 0 . 00 pg / ml ( 1 ) 226 ; median IQR ) ( TNF alpha , 20 , 576 pg / m ( 1 ) 11 , 637 , P < 0 . 0001 ; IFN gamma , 1 , 048 2 , 428 , P = 0 . 0051 , Mann Whitney U test ) . ^^^ Production in patients after surgery had increased ( TNF alpha : 17 , 620 pg / ml ( 1 ) 7 , 986 ; IFN gamma . 410 pg / ml ( 1 ) 2 , 696 ; mean s . d .  ) and were no longer significantly reduced when compared to controls ( TNF alpha , P = 0 . 28 ; IFN gamma , P = 0 . 76 ) . ^^^ Production of TNF alpha and IFN gamma prior to surgery were reduced to a greater extent in patients with Dukes ' stage C tumours compared to those with Dukes ' stage A and B stage . ^^^ Monocytes / macrophages from patients with colorectal carcinoma are refractory to LPS stimulation as reflected by reduction in TNF alpha and IFN gamma production and this is more pronounced in patients with advanced stage tumours . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
No difference in cytokine levels for TNF alpha , IFN gamma , G CSF , IL 10 , IL 6 was detectable between CML CD14+ CCM and control CCM derived from short and long term cocultures . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These data demonstrate that the large majority of T clones are principally CD 4 T cells ; all the clones secreted TNF alpha and a large majority produced IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The detection of tumor specific T cells in immunized cancer patients usually relies on lengthy and difficult CTL assays ; we now report on flow cytometry to detect the intracellular cytokines interleukin 2 ( IL 2 ) , IL 4 , IFN gamma , and tumor necrosis factor alpha ( TNF alpha ) produced by CD4+CD69+ and CD8+CD69+ activated T cells after MUC 1 antigen stimulation . ^^^ After stimulation in vitro with MUC 1 variable number of tandem repeats peptides , CD8+CD69+ T cells from all immunized patients generated 3 9 times higher levels of TNF alpha ( P < 0 . 038 ) and IFN gamma ( P < 0 . 010 ) than did cells from 12 normal subjects ; minor increases in IL 4 occurred . ^^^ By contrast , CD4+CD69+ cells showed no overall alteration in TNF alpha and IFN gamma cytokine production , although in some patients , their measurement was informative ; the measurement of IL 2 was not useful in either CD4+CD69+ or CD8+CD69+ cells . ^^^ We conclude that in MUC 1 immunized patients , the measurement of TNF alpha and IFN gamma in activated CD69+CD8+ T cells may be indicative of their immune status . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
C5a induced adhesion of both neutrophils and eosinophils to unstimulated HUVEC or HBEC , and adhesion was further enhanced when HUVEC and HBEC were `` primed ' ' with TNF alpha and IFN gamma , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The reactivation of CIA by LPS was associated with increases in anti CII IgG and IgG2a antibodies as well as various cytokines including IL 12 , IFN gamma , IL 1beta , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Donor activated spleen CD8+ T cells from nave nonimmunized IFN alpha fed animals suppressed actively induced EAE in recipients and showed decreased IFN gamma and TNF alpha proinflammatory secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Among the eight EBV positive cases , interferon gamma ( IFN gamma ) , lymphotoxin beta ( LT beta ) , interleukin 10 ( IL 10 ) , tumour necrosis factor alpha ( TNF alpha ) , transforming growth factor beta 1 ( TGF beta 1 ) , and IL 1 receptor a ( IL Ra ) were frequently detectable . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokines studied included : interleukin 1beta ( IL 1beta ) , interleukin 6 ( IL 6 ) , interleukin 8 ( IL 8 ) , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) . ^^^ The results show : a ) significant differences in the stimulated versus non stimulated levels of IL 1beta , IL 6 , IL 8 and TNF alpha but not of IFN gamma production by patients , and in the relatives ' stimulated versus non stimulated levels of IL 1beta , IL 6 and IFN gamma ; b ) no difference in the spontaneous cytokine production between any two groups ; and c ) significant increases in the patients ' stimulated cytokines compared to the Caucasian and Indian controls ( P < or =0 . 050 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Similar types of changes were seen in whole lung protein concentrations for TNF alpha , IL 10 , IL 12 p 40 , IFN gamma , and IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 9 protects mice from Gram negative bacterial shock : suppression of TNF alpha , IL 12 , and IFN gamma , and induction of IL 10 . ^^^ The protective effect of IL 9 was correlated with marked decreases in the production of the inflammatory mediators TNF alpha , IL 12 , and IFN gamma , as well as the induction of the anti inflammatory cytokine IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Aggregation of Fc gamma RI on human mast cells , and only after IFN gamma exposure , led to significant degranulation as evidenced by histamine release ( 24 . 5 + / 4 . 4 % ) : and up regulation of mRNA expression for specific cytokines including TNF alpha , GM CSF , IL 3 and IL 13 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of interleukin 6 ( IL 6 ) , interferons alpha ( IFN alpha ) and gamma ( IFN gamma ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , tumour necrosis factor alpha ( TNF alpha ) , and transforming growth factor beta 1 ( TGF beta 1 ) were investigated by ( 125 ) 1 deoxyuridine ( ( 125 ) IUdR ) incorporation assay . ^^^ After 48h in culture , TGF beta 1 , TNF alpha , IFN alpha and IL 6 showed a clear inhibitory effect on ( 125 ) IUdR incorporation ( P < 0 . 005 ) , and IFN gamma and GM CSF caused even more significant inhibition ( P < 0 . 001 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The relationship between NO production with the pro inflammatory cytokines interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) and interleukin 1beta ( IL 1beta ) was evaluated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro stimulation of bronchoalveolar lavage cells from CBD subjects causes the production of high levels of TNF alpha , IFN gamma and IL 6 . ^^^ The addition of 10U or 100U of rMu IFN gamma to Be stimulated 12j cells further increased TNF alpha production 1 . 5 4 fold ( 1 . 6+ / 0 . 1 ng / ml ) respectively . ^^^ The 12j cell line , contrasted with other macrophage hybrids that do not respond to Be stimulation , may provide a useful tool to evaluate the mechanisms by which Be stimulates macrophage cytokine production , and by which T cell derived IFN gamma amplifies TNF alpha production in granulomatous diseases . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We tested the hypothesis that beryllium ( Be ) could stimulate H36 . 12j cell ( 12j ) TNF alpha production by transcription factor mediated pathways similar to those induced by either LPS or IFN gamma stimulation . ^^^ Recombinant Mu IFN gamma ( 10 U ) stimulated 12j cell TNF alpha at lower levels ( 284+ / 31 pg / ml ) while rMu IFN gamma + Be stimulated 12j cells produced 1195+ / 225 pg / ml TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Enzyme linked immunosorbent assay analysis of liver homogenates from both groups of mice at the 24 hours time point revealed that liver tissue from CCR 2 ( / ) mice contained significantly greater amounts of immunoreactive IFN gamma and TNF alpha . ^^^ The in vivo immunoneutralization of IFN gamma or TNF alpha significantly attenuated APAP induced liver injury in CCR 2 ( / ) mice and increased hepatic IL 13 levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha augmented the IFN gamma induced effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here we report on the parallel involvement of TNF alpha , IL 6 , IFN gamma and IL 10 in MS and controls , using enzyme linked immunospot ( ELISPOT ) assays to detect and enumerate cytokine secreting mononuclear cells ( MNC ) prepared from blood and , for IL 6 and IL 10 , from CSF without in vitro stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Other cytokine mRNAs were produced in variable amounts and percentage of overall production of each cytokine message was in the following descending rank : LPS : IL 1alpha , TNF alpha , interferon ( IFN ) gamma , IL 10 , IL 12 p 35 , IL 6 , IL 12 p 40 and TNF beta ; PHA P : IL 1alpha , TNF alpha , IL 10 , IFN gamma , IL 12 p 40 and TNF beta ; HEWL : TNF alpha , IL 1alpha , IFN gamma , IL 10 , IL 6 , IL 12 p 40 , TNF beta and IL 12 p 35 and PPD : IL 1alpha , IFN gamma , TNF alpha and IL 10 . ^^^ Time course response of cytokines revealed early ( IL 1alpha , 10 , TNF alpha ) and intermediate ( IL 12 p 40 , TNF beta , IFN gamma ) responses for PHA P and early ( IL 1alpha , 6 , 10 , IL 12 p 35 , IL 12 p 40 , TNF alpha ) , intermediate ( TNF beta , IFN gamma ) and late ( IL 1alpha , 6 ) for LPS . ^^^ Cytokine mRNA response induced by HEWL was early ( IL alpha , IFN gamma ) , intermediate ( IL 10 , IL 12 p 40 , TNF beta ) , late ( IL 1alpha , IL 12 p 35 ) and very late ( IL 1alpha , 6 , 10 , IL 12 p 40 , TNF alpha ) . ^^^ In Bacillus Calmette Gurin ( BCG ) sensitized pigs , stimulation of LNs with PPD induced message for IL 1alpha , 10 , TNF alpha and IFN gamma which peaked at 24h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Analysis of C57BL / 6 mice acutely infected with lymphocytic choriomeningitis virus ( LCMV ) by using intracellular cytokine staining revealed a high frequency ( 2 to 10 % ) of CD 4 ( + ) T cells secreting the Th 1 associated cytokines interleukin 2 ( IL 2 ) , gamma interferon ( IFN gamma ) , and tumor necrosis factor alpha , with no concomitant increase in the frequency of CD 4 ( + ) T cells secreting the Th 2 associated cytokines IL 4 , IL 5 , and IL 10 following stimulation with viral peptides . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Intracellular staining for TNF and IFN gamma detects different frequencies of antigen specific CD 8 ( + ) T cells . ^^^ Activated CD 8 ( + ) T cells are able to induce cytolysis of infected cells ( perforin and CD 95 CD95L mediated pathways ) and also elaborate cytokines , including IFN gamma and TNF after appropriate MHC class 1 peptide recognition . ^^^ Here , using intracellular cytokine staining , we compare detection of IFN gamma and TNF in the analysis of pathogen specific CD 8 ( + ) T cell lines and CD 8 ( + ) T cells after primary viral infection ( LCMV ) or secondary bacterial infection ( Listeria monocytogenes ) . ^^^ Under multiple conditions and with multiple epitopes , we find that staining for intracellular IFN gamma consistently detects a higher frequency of antigen specific CD 8 ( + ) T cells than detection of intracellular TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human tuberculous granulomas from five adults undergoing surgery for hemoptysis were analyzed by nonradioactive in situ hybridization for tumor necrosis factor alpha ( TNF alpha ) , gamma interferon ( IFN gamma ) , and interleukin 4 ( IL 4 ) gene expression . ^^^ TNF alpha mRNA expression was highest in patients with both IFN gamma and IL 4 mRNA . ^^^ Populations of CD 68 positive macrophage like cells within the granulomas produce mRNA for TNF alpha , IFN gamma , and IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The quantitative expression of genes for CD 14 , interferon gamma receptor ( IFN gamma R ) , tumor necrosis factor alpha ( TNF alpha ) , integrin alpha 4 beta 1 ( VLA 4 ) , tumor necrosis factor receptor p 55 ( TNFR 1 ) and CD 44 ( standard form ) was examined in 9 patients with primary or secondary leg lymphedema before and after phase 1 of CDP . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Primary culture of bovine brain cells was examined for its susceptibility to Neospora caninum infections , and this model was used to investigate the effects of bovine interferon gamma ( IFN gamma ) and tumor necrosis factors alpha ( TNF alpha ) on tachyzoite growth . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using a cell surface specific ICAM 1 adhesion assay we found that in human astrocytoma cells TNF alpha , interferongamma ( IFN gamma ) and PRL increased cell surface ICAM 1 expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Crg 2 has been shown to be inducible by IFN , TNF , IL 1 and LPS , whereas the expression of MuMig is thought to be strictly dependent on IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The evaluation of the cytokine profile ( interleukin 5 [ IL 5 ] , IL 10 , gamma interferon [ IFN gamma ] , and tumor necrosis factor alpha ) in response to these antigens showed inverse correlations between the degree of infection and IFN gamma levels in PBMC supernatants stimulated with paramyosin ( P < 0 . 05 ) and IrV 5 ( P < 0 . 01 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MCP and CD 59 expression was not consistently affected by IL 1alpha , TNF alpha , or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effect of NiSO ( 4 ) on the cytotoxic activity of the pulmonary natural killer ( NK ) cells and alveolar macrophages ( AM ) , as well as the pulmonary production of cytokines such as alpha tumor necrosis factor ( TNF alpha ) and gamma interferon ( IFN gamma ) , were examined 1 , 2 and 7 days later . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The following were measured after the experiment : ( 1 ) messenger RNA ( mRNA ) expression of tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , interleukin 1beta ( IL 1beta ) , and IL 6 in the spleen , thymus , lung , and liver by a semiquantitative reverse transcription polymerase chain reaction method , ( 2 ) inflammatory cytokines in the plasma and supernatant of cultured splenic lymphocytes by enzyme linked immunosorbant assay , ( 3 ) nitric oxide ( NO ) product , NO 2 / NO3 , in the plasma and supernatant of cultured splenic lymphocytes by the Griess method , and ( 4 ) survival rate by the Kaplan Meier method . ^^^ RESULTS : The mRNA expression of TNF alpha was significantly decreased in the spleen and lung ( p < . 01 , p < . 05 ) , IFN gamma in the lung ( p < . 05 ) , IL 1beta in the spleen ( p < . 05 ) , and IL 6 in the thymus and liver ( p < . 05 , p < . 05 ) in the arginine group when compared with the control group . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The involvement of type 1 or type 2 cell control on the production of cytokine mRNAs was analyzed in lymph node cells by quantitative RT PCR , together with the analysis of a wide range of cytokine mRNAs after STZ injection ( IL 1alpha , IL 1beta , TNF alpha , IFN gamma , IL 2 , IL 2 receptor , IL 4 , IL 5 , IL 6 , IL 10 and IL 12 ) . ^^^ STZ induced a low production of interleukin ( IL ) 2 mRNAs , a mild increase in IL 1alpha and IL 6 mRNAs production , and a dramatic increase in IFN gamma , IL 1beta , TNF alpha , IL 12 and IL 2 receptor mRNAs , which correlated with positive PLN responses . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of mRNA for interferon gamma ( IFN gamma ) , interleukin ( IL ) 4 , IL 10 , tumor necrosis factor alpha ( TNF alpha ) and transforming growth factor beta ( TGF beta ) was assessed by polymerase chain reaction whereas secretion of cytokine protein was measured by ELISA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
As part of an investigation on potentiation of the cytotoxic activity of human papillomavirus E 7 specific CD 8 ( + ) cytotoxic T lymphocytes ( CTL ) for adoptive transfusions to cervical cancer patients , we found that IL 10 in combination with IL 2 , unlike several other combinations , including IL 2 with IL 12 , gamma interferon ( IFN gamma ) , tumor necrosis factor alpha , and transforming growth factor beta , was able to consistently increase cytotoxicity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Subjects in this study exhibited significant increases ( p < 0 . 05 ) in the frequency of helper T memory cells expressing interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) in response to HIV 1 antigens in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines including tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) are known to be elevated systemically and locally under these conditions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found that purified uncultured WC1+ T cells express TNF alpha , CD 28 , CTLA 4 and IL 2R alpha mRNA transcripts but do not express those for IL 2 , IL 4 , IL 6 , IL 10 and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Functional activation of the cellular immune response was also evident with marked cerebral expression of the IFN gamma , TNF , and IL 1alphabeta genes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma , TNF alpha , and IL 1beta mRNA were reduced by the treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma and TNF regulate macrophage expression of the chemotactic S 100 protein S100A8 . ^^^ We show that the kinetics of induction of S100A8 mRNA in elicited murine macrophages ( Mac ) by LPS , IFN gamma , and TNF were distinct from the C C chemokines monocyte chemoattractant protein 1 ( MCP 1 ) , macrophage inflammatory protein 1alpha ( MIP 1alpha ) , and RANTES . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Arteries incubated for 6 h with interferon gamma ( IFN gamma , 10 ng ml ( 1 ) ) and tumour necrosis factor alpha ( TNF alpha , 5 ng ml ( 1 ) ) exhibited greater relaxation to BK ( E ( max ) 50 . 0+ / 5 . 1 % ) than polymyxin B treated controls ( E ( max ) 33 . 1+ / 4 . 0 % ) , but did not differ in their response to PE . 4 . ^^^ Prolonged incubation ( 16 h ) with endotoxin ( 3 microg ml ( 1 ) ) did not alter the response to PE , however incubation with IFN gamma ( 10 ng ml ( 1 ) ) , TNF alpha ( 5 ng ml ( 1 ) ) and interleukin 1beta ( 20 ng ml ( 1 ) ) for 16 h increased PE log EC ( 50 ) from 6 . 44+ / 0 . 09 to 6 . 10+ / 0 . 11 . 5 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TGF beta , GM CSF , c kit ligand , and IL 7 are produced constitutively and IL 1beta , IL 6 , IL 8 , TNF alpha , IFN gamma , and MIP 1 alpha are upregulated upon stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interestingly , proinflammatory cytokines such as IL 1beta or IFN gamma as well as LPS greatly decreased CD 59 in SK N SH and to a lesser extent in T98G whereas TNF alpha did not significantly alter it . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In C 6 glioma cells , tumor necrosis factor alpha ( TNF alpha ) concomitantly potentiated the stimulation of nitric oxide ( NO ) and BH ( 4 ) production induced by IFN gamma and interleukin 1beta . ^^^ Surprisingly , although TNF alpha increased BH ( 4 ) synthesis and GTPCH activity , neither BH ( 4 ) nor GTPCH expression was affected by C ( 2 ) ceramide or sphingomyelinase in IFN gamma and interleukin 1beta stimulated cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A delayed production of IFN gamma , TNF alpha , IL 18 and IL 10 and milder histological alterations in the liver were observed in these animals . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To investigate the role of interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) in the resistance to Paracoccidioides brasiliensis ( Pb ) infection , mice with homologous disruption of the IFN gamma ( GKO ) or TNF alpha receptor p 55 ( p55KO ) were infected with the parasite . ^^^ Compared with WT , splenocytes from infected GKO mice cultured with the Pb F 1 fraction produced lower TNF alpha levels , whereas leukocytes from infected p55KO mice produced similar amounts of TNF alpha but higher levels of IFN gamma . ^^^ Taken together , these findings suggests that endogenous TNF alpha , acting through the p 55 receptor , and IFN gamma mediate resistance to Pb infection and induce NO production that determines marked T cell unresponsiveness . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
By contrast , the Th 1 cytokine interferon gamma ( IFN gamma ) downregulated KS IR , both when applied alone or in combination with either GM CSF , M CSF , or TNF alpha . ^^^ In vivo , the intracerebroventricular administration of IFN gamma , but not TNF alpha , to healthy rats led to an almost complete disappearance of KS IR from ramified brain microglia . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To assess the role of specific BCG induced cytokines , urinary HDMEC proliferation assays were repeated in the presence of neutralizing antibodies to tumor necrosis factor alpha ( TNF alpha ) , interferon inducible protein 10 ( IP 10 ) , and / or interferon gamma ( IFN gamma ) . ^^^ RESULTS : Urinary IFN gamma , IP 10 , TNF alpha , and vascular endothelial growth factor ( VEGF ) were induced to nanogram / ml amounts by BCG treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha does not induce 1 TAC mRNA alone , but does act synergistically with IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma as well as the GCs , Dexamethasone and Budesonide , inhibited TNF alpha induced IL 8 secretion in a dose dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The inflammatory cytokines interleukin 1beta ( IL 1beta ) ( 100 microg / ml ) and tumour necrosis factor alpha ( TNFalpha ) ( 10 ng / ml ) did not affect IFN gamma mRNA expression or protein production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have previously shown that IFN gamma induced NO production is augmented by herpes simplex virus type 2 ( HSV 2 ) infection through autocrine tumour necrosis factor ( TNF ) alpha secretion and is inhibited by interleukin ( IL ) 4 . ^^^ IL 4 reduced virus induced TNF alpha secretion and nuclear factor ( NF ) kappaB activation significantly , but less in cells concomitantly treated with IFN gamma . ^^^ The data show that inhibition of IFN gamma induced NO production by IL 4 is impaired by HSV 2 infection due to autocrine TNF alpha mediated NF kappaB activation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cells were stimulated in vitro with interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , or a combination of both . ^^^ We observed upregulation of FasL expression under the influence of IFN gamma , while adding TNF alpha alone to the culture medium had no effect . ^^^ IFN gamma and TNF alpha acted synergistically , leading to an increased FasL expression that reached its maximum 70 h after cytokine exposure , as shown by FACS analysis , SDS PAGE , and Western blot . ^^^ Fas expression on untreated SC showed fluctuating levels , while addition of IFN gamma and TNF alpha suppressed Fas expression completely . ^^^ These findings are in accord with recently published results showing Fas and FasL expression on malignant human cells , derived from brain tumors and upregulation of FasL on astrocytes after exposure to IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The promonocyte THP 1 cell line , previously used to model the effect of IL 1 on IDO mRNA expression , was treated with IFN gamma and increasing concentrations of LPS or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) , which primes macrophages for TNF alpha dependent cellular cytotoxicity , potentiated the effect of LPS by sustaining enhanced intracellular pools of TNF alpha and also promoted redistribution of TNF alpha into post Golgi vesicular compartments . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
BACKGROUND : Psychologic stress in humans induces the production of proinflammatory cytokines , such as interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and interleukin 6 ( IL 6 ) , and that of the negative immunoregulatory cytokine , IL 10 . ^^^ We determined the omega 6 and omega 3 fractions in serum phospholipids as well as the ex vivo production of IFN gamma , TNF alpha , IL 6 , IL 10 , and IL 5 by diluted whole blood stimulated with polyclonal activators . ^^^ RESULTS : Academic examination stress significantly increased the ex vivo , stimulated production of IFN gamma , TNF alpha and IL 10 , and the IFN gamma / IL 5 production ratio . ^^^ Subjects with lower serum omega 3 PUFA levels or with a higher omega6 / omega3 ratio had significantly greater stress induced TNF alpha and IFN gamma responses than subjects with higher serum omega 3 PUFAs and a lower omega6 / omega3 ratio , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The endothelial cells were stimulated by interleukin 1beta ( IL 1beta ) , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) with or without TGF beta ( 1 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To investigate the involvement of interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) , interleukin 4 ( IL 4 ) and interleukin 10 ( IL 10 ) in MG , the mRNA levels of these cytokines in peripheral blood mononuclear cells without stimulation in vitro were quantified by competitive reverse transcription polymerase chain reaction technique . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma induced sPLA ( 2 ) IIA expression was antagonized by tumor necrosis factor ( TNF ) alpha and interleukin ( IL ) 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lovastatin , a drug commonly used in the clinic to treat hypercholesterolemia , has previously been reported to exert antitumor effects in rodent tumor models and to strengthen the antitumor effects of immune response modifiers ( tumor necrosis factor alpha and IFN gamma ) or chemotherapeutic drugs ( cisplatin ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These cell lines were ( 1 ) the poorly immunogenic VKCK cell line and ( 2 ) its two derived cell lines VKCK / RM4 tumor necrosis factor alpha ( TNF alpha ) and VKCK / RM4 interferon gamma ( IFN gamma ) engineered to secrete TNF alpha and IFN gamma , respectively . ^^^ Our results indicate that regression of tumor cells engineered to secrete cytokines TNF alpha and IFN gamma is related to a shift from a host type 2 to a type 1 cytokine profile . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lethal shock can be associated with excessive secretion of cytokines such as tumor necrosis factor ( TNF ) and gamma interferon ( IFN gamma ) . ^^^ LPS induced IFN gamma production was abrogated , yet induction of IL 12 and TNF was not affected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These bovine insulin primed CTL displayed a type 0 CTL phenotype , producing IL 4 , IL 5 , IL 10 , low levels of IFN gamma , but no TNF alpha . ^^^ By contrast , CTL generated from C57BL / 6 mice primed with OVA in CFA produced IFN gamma and TNF alpha but no IL 4 , IL 5 , or IL 10 and therefore were classified as type 1 CTL . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These studies show that coincident expression of CD40L enhances the Th 1 ( IL 2 and IFN gamma ) cytokine responses , increases the expression of TNF alpha and NO , accelerates virus clearance , and increases the anti F and anti G Ab responses . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The surface expression of CXCR 4 in eosinophils was up regulated by IFN gamma , TNF alpha , and TGF beta while it was down regulated by IL 4 and eosinophil directed hemopoietins such as IL 5 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Intestinal mucosal inflammation was effectively prevented , as revealed by abrogated leukocyte infiltration and decreased CD 54 expression and strongly diminished mRNA levels of the proinflammatory cytokines IFN gamma , TNF , and IL 12 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Early myeloid cells are high producers of nitric oxide upon CD 40 plus IFN gamma stimulation through a mechanism dependent on endogenous TNF alpha and IL 1alpha . ^^^ Both cytokines are transcribed when Fr 3 WGA ( + ) cells are stimulated by CD 40 signaling plus IFN gamma , although TNF alpha remains below detection limits in stimulated Fr 3 WGA ( + ) cell cultures . ^^^ The results point to early myeloid cells as an important cell source of NO once triggered by activated T cells through CD 40 and IFN gamma derived signals , in a mechanism involving the production of TNF alpha and / or IL 1alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Colons of IFN gammaR 1 ( / ) mice contained a greater number of cells , represented by macrophages and CD 4 ( + ) T cells ; caudal lymph node cells produced more IFN gamma and TNF alpha upon stimulation in vitro . ^^^ IFN gamma and TNF alpha ) by caudal lymph node T lymphocytes was enhanced rather than decreased in IFNgammaR 1 ( / ) mice with no evidence for default Th 2 development . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1 in moderate to high concentrations and TNF and IFN gamma all inhibited thyroid cell function . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Gamma interferon ( IFN gamma ) alone or in combination with interleukin 1 ( IL 1 ) , IL 6 , or tumor necrosis factor alpha significantly inhibits the growth of T . gondii in murine astrocytes , suggesting these are important nonimmune effector cells in the brain . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Since gamma interferon ( IFN gamma ) stimulates cytotoxic cells , including antigen specific CTL which may control virus replication by secretion of antiviral cytokines such as tumor necrosis factor alpha and IFN gamma , we have investigated the mechanism of HSI by analyzing the role of IFN gamma for HSI in IFN gamma gene deleted ( IFN gamma ( / ) ) mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In combination with tumor necrosis factor alpha ( TNF alpha ) ( 50 ng / ml ) , IFN gamma elicited severe cytotoxicity and induced the expression of the inducible isoform of nitric oxide synthase ( iNOS ) mRNA . ^^^ TNF alpha did not affect STAT 1 activation , but stimulated DNA binding and transcriptional activity of NF kappaB , both of which were further increased by IFN gamma . ^^^ These effects of IFN gamma and TNF alpha seem physiologically relevant , because either inhibition of STAT 1 by the tyrosine kinase inhibitor herbimycin A or that of NF kappaB by sulfasalazine resulted in the reduction of iNOS mRNA expression . ^^^ In conclusion , IFN gamma activates STAT 1 and potentiates TNF alpha induced NF kappaB activation in INS 1 cells , thereby inducing iNOS and cell destruction . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using a model of ex vivo liver cold ischemia and reperfusion , we assessed liver injury , tumor necrosis factor ( TNF ) and interferon gamma ( IFN gamma ) release from livers of balb / c mice , nude mice , nude mice reconstituted with T cells , and gadolinium balb / c pretreated mice . ^^^ TNF and IFN gamma were released time dependently and paralleled liver injury after reperfusion of cold preserved livers . ^^^ Reperfused livers from nude or gadolinium pretreated mice disclosed a dramatic decrease in TNF and IFN gamma release . ^^^ Only donor pretreatment with IL 10 or IL 10 infusion during reperfusion led to a significant decrease in liver injury , TNF , and IFN gamma release ( 66 % or 41 % , 95 % or 94 % , and 70 % or 70 % , respectively ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Am 80 inhibited the levels of expression in mice ears of interferon gamma ( IFN gamma ) and interleukin 6 ( IL 6 ) , but not tumor necrosis factor alpha ( TNF alpha ) or interleukin 4 ( IL 4 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , our data suggest that interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) are involved in this T cell mediated DC maturation . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The L NMMA induced enhancement was due to the prolonged survival of BCG in macrophages , because no enhancement of cytotoxicity was observed and neither IFN gamma nor TNF alpha production was significantly enhanced by killed BCG . ^^^ The depletion of T cells from PEC reduced the production of both IFN gamma and TNF alpha , as well as the enhancement of cytotoxicity induced by viable BCG plus L NMMA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Time dependent increases in DCF fluorescence as a measure of reactive oxygen load were quantified in single cells after incubation with TNF alpha , IL 1 and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Kidneys were weighed and sectioned for histo pathology and in situ hybridization for mRNA of IL 1beta , TNF alpha , IL 4 , IL 6 , IL 10 , IL 12 , TGF beta and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Assessment of supernatants from MDDC stimulated CB and adult blood T cells showed no significant difference in the levels of either IFN gamma or TNF alpha , but CB T cell supernatants did show a significant lack of detectable IL 2 . ^^^ Intracellular cytokine analysis revealed that dividing CB T cells had been primed to produce IFN gamma , TNF alpha , and IL 2 on restimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma induction of murine 1 TAC is markedly enhanced by costimulation with LPS or IL 1beta in RAW cells and by TNF alpha in both RAW cells and Swiss 3T3 fibroblasts . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This acute mortality was linked to overexpression of the proinflammatory mediators IFN gamma , TNF alpha , and inducible NO and the formation of nonfibrotic granulomas . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine treated mice produced significantly more IFN gamma , TNF alpha , and IL 6 than the controls . ^^^ Interestingly , IL 18 treated mice produced more TNF alpha and IL 6 , but less IFN gamma , compared with mice treated with IL 12 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The microvascular damage and leukocytic infiltration elicited by TNF were enhanced by porcine IFN gamma , suggesting that xenografts may be less prone to cytokine mediated injury due to the species restricted effects of recipient IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cotreatment of cells with interferon gamma ( IFN gamma ) and lipolysaccharide ( LPS ) during T . gondii infection induced TNF alpha production and decreased the number of parasitized cells ( invasion index ) . ^^^ Thus , in contrast to the results obtained in murine models but in accordance with those observed in other human models , the present study shows for the first time in human monocytic cells that T . gondii does not induce any TNF alpha secretion and inhibits TNF alpha production induced by IFN gamma / LPS . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Therefore , we determined tumor necrosis factor alpha ( TNF alpha ) , interleukin 1beta ( IL 1beta ) , IL 4 , IL 10 , and interferon gamma ( IFN gamma ) mRNA and serum levels in HIV infected patients under nonstimulated conditions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We report here the action of interleukin 1 ( IL ) 1 and , for the first time , lipopolysaccharide ( LPS ) , platelet derived growth factor ( PDGF ) , IL 6 , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) on the C / EBP expression profile and functional DNA binding activity in primary rat MC . ^^^ Both cell type and stimulus specific regulation of C / EBP mRNA expression and DNA binding activity were identified , with C / EBPalpha being induced by LPS , C / EBPbeta by LPS , IL 1 , TNF alpha and C / EBPdelta by LPS , IL 1 , IFN gamma , TNF alpha and PDGF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , the expression of JunB , c jun and c fos , but not JunD , was increased by LPS , TNF alpha , IFN gamma and IL 1 , albeit with different kinetics and magnitude of induction . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Therefore , we examined whether combination of these three cytokines with IL 2 , IL 4 , IL 6 , IL 10 , IL 12 , IFN alpha , IFN gamma , GM CSF , M CSF and TNF alpha may yield higher ADCC than obtained by the application of single cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
EAE amelioration was associated with decreased infiltration of ED1+ macrophages and CD4+ T cells within the central nervous system , and with decreased interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) and enhanced IL 4 , IL 10 and transforming growth factor beta ( TGF beta ) responses by lymph node cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When the stimulator PECs were pretreated with TGF beta 2 and then pulsed with OVA , responding OT 1 T cells proliferated even more swiftly , but they secreted significantly less IFN gamma , IL 2 , and TNF alpha , and no IL 4 or IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
No detectable increases in serum TNF alpha , IL 1 beta or IFN gamma were observed in either patient . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To assess host regulation of subclinical C . piliforme infections and the effects of those infections on laboratory mice , the expression of the pro inflammatory cytokines tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) was evaluated at 0 , 1 , 3 , 7 , 14 and 28 days after inoculation with C . piliforme . ^^^ Hepatic TNF alpha and IFN gamma mRNA and serum protein levels were similarly elevated in both mouse strains 1 day after inoculation with both C . piliforme strains , as evaluated by reverse transcription PCR and enzyme linked immunosorbent assays , respectively . ^^^ Elevation of IFN gamma persisted for 14 days after inoculation ; TNF alpha remained elevated at 28 days after inoculation . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 2 , IL 4 , IL 6 and IL 10 promoted the viability of PBMC in cultures while IL 1alpha or IFN gamma had no obvious impact and TNFalpha gave conflicting results . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have studied the impact of short term treatment with interferon ( IFN ) beta1b of relapsing remitting ( RR ) multiple sclerosis ( MS ) patients ' blood levels of type 1 and type 2 cytokines such as IFN gamma , interleukin ( IL ) 1beta , IL 2 , IL 4 , IL 6 , IL 10 and tumour necrosis factor ( TNF ) alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Additionally , we have determined by flow cytometry that CD4+ T cells from these individuals are the main lymphocyte subpopulation producing IFN gamma and TNF alpha . ^^^ Exogenous IL 10 suppressed T cell proliferation and neutralization of endogenous IL 10 restored lymphocyte activation and enhanced IFN gamma and TNF alpha production in chronically infected patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The DP 4 inhibitors Lys [ Z ( NO 2 ) ] thiazolidide and Lys [ Z ( NO 2 ) ] pyrrolidide suppress in a dose dependent manner DNA synthesis and IFN gamma , IL 4 , and TNF alpha production of the antigen stimulated TCC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Peripheral blood mononuclear cells cultured in vitro with IL 2 secrete secondary cytokines such as IL 1 , TNF alpha , and interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine detection in patients ' sera by ELISA showed significant increases ( p < 0 . 05 ) for IFN gamma and TNF alpha but not for IFN alpha four h post vaccination . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated whether the biological response modifiers like IFN alpha , IFN gamma and TNF alpha could enhance the cytotoxic action of cisplatin on cervical carcinoma cell lines in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) and interleukin 1 ( IL 1 ) increased endothelial adhesion molecule ( CAM ) expression , whereas interferon gamma ( IFN gamma ) induced little up regulation of CAM . ^^^ However , both TNF alpha and IFN gamma strongly facilitated T cell migration , which was completely inhibited by pertussis toxin and both greatly increased TEM to RANTES , MIP 1alpha , and SDF 1 selectively of memory but not naive T cells . ^^^ Tumor necrosis factor alpha ( TNF alpha ) and interleukin 1 ( IL 1 ) increased endothelial adhesion molecule ( CAM ) expression , whereas interferon gamma ( IFN gamma ) induced little up regulation of CAM . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In whole blood stimulated with heat killed B . pseudomallei , neutralization of IFN gamma and tumor necrosis factor alpha ( TNF alpha ) partly attenuated IP 10 and Mig release , while anti interleukin 12 ( IL 12 ) and anti IL 18 had a synergistic effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
T . cruzi infected IL 10 ( / ) mice showed reduced parasitemia accompanied by increased systemic release of gamma interferon ( IFN gamma ) , IL 12 , and reactive nitrogen intermediates and overproduction of tumor necrosis factor alpha ( TNF alpha ) . ^^^ Since elevated serum IL 12 and IFN gamma concentrations were not affected by the administration of antiserum , these studies suggest that TNF alpha is the direct mediator of this toxic shock syndrome . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Animals were euthanized at 2 or 4 h postinoculation ( p . i . ) , and tissues were collected to assess PIC gene expression using antisense RNA probes specific for bovine interleukin 1alpha ( IL 1alpha ) , IL 1beta , IL 6 , gamma interferon ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) along with the beta actin ( beta Act ) housekeeping gene . ^^^ IFN gamma and TNF alpha genes were not increased in P . haemolytica inoculated CD 18 ( ) cattle lungs compared to the PFS inoculated control lungs at 4 h p . i . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These findings support the rationale for combining a chemotherapy agent with an immunotherapy agent for the treatment of metastatic disease , and suggest a role for TNF alpha and IFN gamma as agents for stimulating the antitumor activity of macrophages . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The action of platelet derived growth factor ( PDGF ) and , for the first time , lipopolysaccharide ( LPS ) , interleukin 6 ( IL 6 ) , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) on AP 1 gene expression in MC was therefore studied . ^^^ Whilst the expression of JunD was not affected by any of the mediators , the mRNA levels of c fos and JunB were induced by LPS , IL 6 , IFN gamma , PDGF and TNF alpha , and that of c jun by LPS , IFN gamma , PDGF and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Synergistic induction of apoptosis in murine hepatoma Hepa 1 6 cells by IFN gamma and TNF alpha . ^^^ In this study , we examined the susceptibility of murine hepatoma Hepa 1 6 cells to undergo IFN gamma and / or TNF alpha induced apoptosis . ^^^ IFN gamma or TNF alpha alone had no demonstrable cytotoxic effects , whereas IFN gamma and TNF alpha in combination induced apoptosis drastically in Hepa 1 6 cells . ^^^ These results suggest that the caspase 3 activation , the release of cytochrome c from mitochondria , and increased caspase 11 gene expression involve in synergistic induction of apoptosis in Hepa 1 6 cells by IFN gamma and TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The ligation of DCs as well as Con A blasts by IL 12 induced the production of GM CSF , IL 1beta , IL 6 , TNF alpha , and IFN gamma at the transcription levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although SDF 1 alpha did not induce mast cell degranulation , it selectively stimulated production of the neutrophil chemoattractant IL 8 without affecting TNF alpha , IL 1beta , IL 6 , GM CSF , IFN gamma , or RANTES production , providing further evidence of the selective modulation of mast cell function by this chemokine . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To determine the factors involved in these events , we depleted T cell subsets or neutralized the inflammatory cytokines IFN gamma , IL 12 , or TNF in TNFRp 55 / mice infected i . v . with M . avium . ^^^ Neutralization of IL 12 , but not of IFN gamma or TNF , also protected M . avium infected TNFRp 55 / mice from granuloma decomposition and from premature death . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of interleukin ( IL ) 1beta , IL 6 , IL 8 , interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) was measured by semiquantitative polymerase chain reaction . ^^^ However , expression of TNF alpha and IL 8 , IFN gamma , and IL 6 and IL 1beta was 2 20 times greater in patients administered 100 % than in those administered 30 % oxygen . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Provided by the evidences that , in cancer patients , the production of Th 1 cytokines including IL 12 , IFN gamma , and TNF alpha , is impaired , we asked whether these cytokines can be useful parameters for cancer detection . ^^^ PHA induced production of IL 12 , IFN gamma and TNF alpha , in cancer was lower than that in control . ^^^ From the results IL 12 , IFN gamma , TNF alpha and Th 1 subset as well as NK activity appear to be promising parameters for cancer detection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There was a trend toward upregulation of IFN gamma mRNA , but no difference in expression of TNF alpha mRNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neonatal and adult rat cardiac fibroblasts cultures in vitro were exposed to interleukin ( IL ) 1beta ( 4 ng / mL ) , tumor necrosis factor alpha ( TNF alpha ; 100 ng / mL ) , IL 6 ( 10 ng / mL ) , or interferon gamma ( IFN gamma ; 500 U / mL ) for 24 hours . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Since the mechanism for their genetic resistance is unknown , we examined the role of interferon gamma ( IFN gamma ) tumor necrosis factor alpha ( TNF alpha ) and inducible nitric oxide synthase ( iNOS ) in the resistance using BALB / c background IFN gamma deficient ( IFN gamma ( / ) ) mice . ^^^ IFN gamma mRNA was detected in brains of only the control mice , whereas mRNA for TNF alpha and iNOS were detected in brains of both strains of mice . ^^^ These results demonstrate that IFN gamma is crucial for genetic resistance of BALB / c mice against TE and that TNF alpha and iNOS are insufficient to prevent TE in the absence of IFN gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Messenger RNAs for six cytokines ( IL 12p40 , IFN gamma , IL 10 , IL 4 , TNF alpha and TGF beta 1 ) expressed in vivo during development of experimental autoimmune uveitis ( EAU ) were quantitated by PCR in ( uncultured ) peripheral lymphoid cells and in the eyes of EAU susceptible Lewis and EAU resistant F 344 rats . ^^^ In ocular tissues , however , only Lewis expressed elevated type 1 and inflammatory cytokines ( IL 12p40 , IFN gamma and TNF alpha ) , coincident with onset and peak of disease . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Alternatively , HAV may indirectly stimulate the T helper 1 ( Th 1 ) type cytokine responses , such as interleukin ( IL ) 2 , IFN gamma and tumour necrosis factor ( TNF ) alpha , which directly promote the antiviral CTL response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In particular , hyperfibrinogenemia and the increase of erytrhocyte aggregation were correlated with the increased generation and release of TNF alpha and IFN gamma ( spontaneous release and IL 2 modulated release ) from natural killer ( NK ) lymphocytes ( CD16+ , CD56+ , CD 3 cells ) of patients with DAT ; whereas a normal cytokine release from NK cells was found in healthy old subjects and in patients with vascular dementia ( VaD ) . ^^^ The association between hemorheological changes and alterations of TNF alpha and IFN gamma release from NK may indicate a potential immunorheologic mechanism associated with cerebrovascular damage in DAT and could suggest the use of vascular protective drugs as support of the main pharmacological and non pharmacological therapy of AD . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Human dermal fibroblasts , human umbilical vein endothelial cells and A 549 human bronchial epithelial cell line cells were stimulated with TNF alpha , IL 4 , IFN gamma or TNF alpha in combination with IL 4 or IFN gamma and the amount of eotaxin production was analyzed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A possible protective effect of fibronectin against toxic effects of TNF alpha and IFN gamma was investigated in cultured trophoblasts isolated from six human term placentas , grown on uncoated and fibronectin coated plastics . ^^^ IFN gamma and increasing doses of TNF alpha resulted in decreasing viability of trophoblast on uncoated as well as fibronectin coated dishes , as shown by 3 [ 4 , 5 dimethylthiazol 2 yl ] 2 , 5 diphenyl tetrazolium bromide ( MTT ) assays , but for each TNF / IFN treatment condition viability on fibronectin was higher ( P < 0 . 001 ) . ^^^ Epidermal growth factor ( EGF ) , a growth factor reported to protect against TNF alpha / IFN gamma induced toxicity , resulted in further increased viability , but not if IFN gamma was included in the treatment . ^^^ We conclude that fibronectin increases viability , but does not completely abolish the cytotoxic action of TNF alpha and IFN gamma on trophoblast . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Colonic mucosal total RNA was assayed for tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , interleukin 2 ( IL 2 ) , and glyceraldehyde 3 phosphate dehydrogenase ( GAPDH ) , internal standard , mRNA by reverse transcriptase polymerase chain reaction . ^^^ Furthermore , GLP 2 reduced the mean band intensity ( MBI ) of TNF alpha ( 0 . 4 + / 0 . 04 in group 2 to 0 in group 3 , P < . 01 ) and IFN gamma ( 0 . 3 + / 0 . 02 in group 2 to 0 in group 3 , P < . 01 ) . ^^^ This is further supported by a significant decrease in gene expression of the inflammatory mediators TNF alpha ( 100 % decrease ) and IFN gamma ( 100 % decrease ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha and IFN gamma regulate expression and function of the Fas system in the seminiferous epithelium . ^^^ Sertoli cells stimulated with TNF alpha and IFN gamma markedly increase the expression of both soluble and membrane bound Fas in a dose dependent manner . ^^^ Taken together , our data suggest a possible regulatory role of TNF alpha and IFN gamma on Fas mediated apoptosis in the testis through disruption of the balance between different forms of Fas . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Indispensable role for TNF alpha and IFN gamma at the effector phase of liver injury mediated by Th 1 cells specific to hepatitis B virus surface antigen . ^^^ Therefore , TNF alpha and IFN gamma , which were produced by HBsAg specific Th 1 cells during the effector phase , appeared to be indispensable in the pathogenesis of fulminant hepatitis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Among cytokines known to modulate mycobacterial growth particularly in murine macrophages ( TNF alpha , IFN gamma , TGF beta , IL 4 ) , only IL 10 modulated the growth in human DC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We examined the effects of the differentiation inducing agents 1 , 25 dihydroxyvitamin D ( 3 ) ( calcitriol ) , phorbol myristate acetate ( PMA ) , the cytokine interferon gamma ( IFN gamma ) , and the tumor necrosis factor alpha ( TNF alpha ) alone and in combination with calcitriol on cell growth and differentiation parameters of cultured nontransformed human mammary epithelial cell ( HMEC ) lines , the chemically transformed HMEC line H 184 A1N4 , and the human mammary carcinoma cell line CAL 51 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We show that the majority of circulating CD 8 ( + ) T cells specific for CMV and HIV antigens are functionally active with regards to the secretion of antiviral cytokines in response to antigen , although a subset of tetramer staining cells was identified that secretes IFN gamma and MIP 1beta but not TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TGF beta 1 induced IL 10 production , slightly decreased TNF alpha production and decreased IFN gamma production . ^^^ The PD 098059 inhibitor decreased both IFN gamma and TNF alpha production and together with TGF beta 1 , it totally blocked IFN gamma , TNF alpha and IL 10 production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There was , however , an inhibition of IFN gamma and TNF alpha production by simazine , metoxuron and mecoprop and of all three cytokines tested by diuron . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
High levels of tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) mRNAs were also detected , and both these cytokines can be harmful to several types of CNS cells , including neurons . ^^^ However , treatment of embryonic motoneuron cultures with TNF alpha or IFN gamma only had a deleterious effect in cultures deprived of neurotrophic factors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our data suggest that this was mediated via specific cellular interactions of T cells and microglial cells since IFN gamma alone was not sufficient to induce axonal damage while such damage was apparent in response to TNF alpha which is released by activated microglial cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The embryotoxic factors used were interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , lipopolysaccharide ( LPS ) , 5 Azacytidine ( 5 AzaC ) and the murine embryotoxic antibody DF 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Late viral gene expression could be reactivated by TNF alpha , GM CSF and IFN gamma treatment of SIVmac 251 infected astrocytes but not by similarly treated SIVmac 251 DeltaNef infected cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha is a potent inducer for IFN inducible protein 10 in hepatocytes and unaffected by GM CSF in vivo , in contrast to IL 1beta and IFN gamma . ^^^ IP 10 mRNA expression in hepatocytes was induced by i . v . administration of TNF alpha , and to a much lesser extent in response to IL 1beta and IFN gamma , whereas Kupffer cells and endothelial cells expressed IP 10 mRNA equivalently in response to these three stimuli . ^^^ On the other hand , JE / MCP 1 mRNA expression was detected only in non parenchymal cells in response to TNF alpha and IL 1beta , but not in response to IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Because of the possible different involvement of these mediators in bacterial infections and tuberculosis , enzyme immunoassay was used to measure comparatively the plasma levels of the proinflammatory cytokines interleukin 1 beta ( IL 1beta ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 6 ( IL 6 ) and interferon gamma ( IFN gamma ) in 25 immunocompetent patients divided into two groups : in 12 patients clinical and microbiological diagnosis showed a chronic bacterial infection and 13 patients had pleuropulmonar tuberculosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma , IL 2 , IL 10 , TNF beta , granulocyte macrophage colony stimulating factor and Fas ligand showed strong positive correlations with CD 3 delta , indicating an association between infiltrating T cells and these cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Following stimulation with LPS , TNF alpha , IFN gamma , and IL 1beta alone or in combination , HBMEC released significant amounts of RANTES and MIP 1beta into the culture supernatants . ^^^ RANTES secretion by HUVEC could be induced only with TNF alpha / IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the current study we investigated the regulatory capacity of reactive oxygen intermediates ( ROIs ) in ICAM 1 activation by stimulating endothelial cells with the pro inflammatory cytokines IL 1 beta , TNF alpha , IFN gamma , IL 2 , and IL 4 prior to antioxidant treatment . ^^^ ICAM 1 was expressed constitutively and upregulated on ECV 304 by IL 1 beta , IL 2 , and IFN gamma and on SKHEP 1 by IFN gamma , IL 1 beta , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Apoptosis was detected in the liver of L . monocytogenes infected mice which had been treated with monoclonal antibody ( mAb ) against tumor necrosis factor alpha ( TNF alpha ) or interleukin 6 ( IL 6 ) , or in TNF alpha ( / ) mice , but not in gamma interferon ( IFN gamma ) ( / ) mice or mice which had been treated with mAb against IL 4 or IL 10 . ^^^ Augmentation of liver apoptosis in mice treated with mAb against TNF alpha or IL 6 or in TNF alpha ( / ) mice correlated with the increase in bacterial numbers in the organ , while no augmentation of apoptosis was observed in the liver of IFN gamma ( / ) mice irrespective of the marked increase in bacterial numbers in the organs , indicating that augmentation of liver apoptosis may not be merely due to the increase in bacterial growth in the organs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study we evaluated the induction of a type 1 ( interferon gamma ( IFN gamma ) ) , a type 2 ( interleukin ( IL ) 10 ) and a primarily macrophage derived cytokine ( tumor necrosis factor ( TNF ) alpha ) in peripheral blood mononuclear cells ( PBMC ) from patients with PCM . ^^^ Cytokines were induced with non specific stimuli phytohaemagglutin ( PHA ) ( induces IL 10 and IFN gamma ) , Lipopolysaccharide ( induces TNF alpha ) and Paracoccidioides brasiliensis antigen ( PbAg ) ( induces IL 10 , IFN gamma and TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In cultured L 6 cells , the expression of mRNA encoding TNFRII and IL 6R receptors was induced by TNF alpha , and all six cytokine receptor mRNA were induced by a mixture of TNF alpha , IFN gamma , and endotoxin ( P < 0 . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Human salivary duct cell line ( HSG ) was cultured with interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 beta ( IL 1 beta ) , interleukin 2 ( IL 2 ) , interleukin 4 ( IL 4 ) , and granulocyte monocyte colony stimulating factor ( GM CSF ) . ^^^ IFN gamma and TNF alpha consistently upregulated constitutive levels of Fas . ^^^ HSG cells expressing Fas antigen in response to IFN gamma or TNF alpha were susceptible to apoptosis by anti Fas antibody . ^^^ CONCLUSION : Our findings suggest that IFN gamma or TNF alpha secreted by infiltrating lymphocytes induces ductal Fas expression and ductal apoptosis in sialoadenitis associated with SS . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PROCEDURE : PBMC were isolated , and concentrations or activities of secreted interleukin ( IL ) 1 , IL 2 , IL 6 , tumor necrosis factor ( TNF ) , and interferon gamma ( IFN gamma ) were measured after in vitro stimulation of cells with concanavalin A ( ConA ) , lipopolysaccharide ( LPS ) , or a whole cell sonicate of M paratuberculosis ( MpS ) . ^^^ Cells from cows with subclinical paratuberculosis produced significantly more TNF and IFN gamma in response to MpS than cells from the other 2 groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha , IL 1alpha , IL 10 , IL 12 , and IFN gamma mRNA persisted into the late stage of myocarditis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Significantly lower number of CD4+ T cells producing IFN g ( p < 0 . 001 ) , TNF alpha ( p=0 . 012 ) and TNF beta ( p=0 . 03 ) and of CD8+ T cells producing IFN gamma ( p < 0 . 001 ) , IL 2 ( p=0 . 005 ) and TNF alpha ( p < 0 . 001 ) were found in CB as compared to PB . ^^^ INTERPRETATION AND CONCLUSIONS : Our data confirm that the cytokines which normally sustain the phagocytic dependent T helper / cytotoxic 1 type immune responses ( IFN gamma , IL 2 and TNF alpha ) and the NK cell / monocyte dependent aspecific responses ( TNF alpha ) are reduced in CB . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RT PCR analysis revealed a marked reduction in the expression of interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) in the brains of these animals . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study examines the effect of IFN beta treatment on the cytokines IL 6 , TNF alpha , IFN gamma and IL 10 ; the metalloproteinases MMP 3 , 7 and 9 and the tissue inhibitor of metalloproteinase 1 ( TIMP 1 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 4 , but not IL 10 or IL 17 , significantly up regulated IFN gamma or TNF alpha induced IP 10 , Mig , and 1 TAC mRNA accumulation in keratinocytes and increased the levels of IP 10 and Mig in keratinocyte supernatants . ^^^ In conclusion , IL 4 exerts a proinflammatory function on keratinocytes by potentiating IFN gamma and TNF alpha induction of IP 10 , Mig , and 1 TAC , which in turn may determine a prominent recruitment of CXCR3+ T lymphocytes at inflammatory reaction sites . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Since species differences have been shown between human and murine IL 18 , the aims of this study were to investigate 1 ) if species homologous IL 18 alone or following IL 12 pre exposure affected rat islet function , 2 ) if IL 18 dose dependently modulated IL 1 beta or interferon gamma ( IFN gamma ) + tumor necrosis factor alpha ( TNF alpha ) actions on islet function , and 3 ) if IL 18 and IL 18 receptor ( IL 18R ) were expressed in rat islet beta cells . ^^^ However , rmIL 18 failed to modulate these effects of IL 1 beta or IFN gamma + TNF alpha . ^^^ IL 18R beta mRNA , which was expressed in human peripheral blood mononuclear cells ( PBMC ) , was not expressed in rat insulinoma ( RIN ) cells or in isolated rat islets , even after exposure to IL 1 beta and / or IFN gamma + TNF alpha or IL 12 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Even though IL 13 reduced TNF alpha secretion by 50 % , this did not impair NF kappa B activation in IFN gamma treated cells infected with HSV 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In response to CVB 3 infection we investigated the time course of iNOS induction in correlation with cytokine mRNA expression ( TNF alpha , IL 1 alpha , IFN gamma , TGF beta ) in the heart of NMRI mice by RT PCR . ^^^ The mRNA of the inflammatory cytokines TNF alpha , IL 1 alpha , IFN gamma appeared at days 1 , 4 , and 7 p . i . , peaked at day 7 p . i . and persisted until day 98 p . i . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the role of human interleukin 15 ( IL 15 ) in this phenomenon and report here that HSV 1 mediated enhanced NK activity was abrogated by neutralizing antibodies for IL 15 but not for other cytokines ( i . e . , IL 2 , IL 12 , gamma interferon [ IFN gamma ] , tumor necrosis factor alpha , or IFN alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The intracellular IL 4 expression in CD4+ lymphocytes from patients with transient hypogammaglobulinemia was comparable to that of control while the number of CD4+ lymphocytes expressing TNF alpha , TNF beta and IFN gamma was elevated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The suppression of lipoprotein lipase expression in J774 . 2 macrophages by IFN gamma and TNF alpha is mediated at the transcriptional level . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of transforming growth factor ( TGF beta 1 ) , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) and interleukin 4 ( IL 4 ) were assessed in lung tissues from patients with tuberculosis . ^^^ TNF alpha , IFN gamma , and IL 4 were absent in sections from all tuberculosis patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The prevention of the lowering of plasma glutamine concentration allows an increased response of lymphocytes to ConA and LPS , as well as an increased production of IL 1 and 2 , TNF alpha , and IFN gamma , possibly linked to the lower incidence of symptoms of infection ( 33 . 84 % ) reported by the supplemented athletes . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Reverse transcriptase polymerase chain reaction ( RT PCR ) and Southern hybridization using 32P labeled cytokine gene specific probes were utilized to detect cytokine mRNA . mRNA transcripts for the Th 1 cytokines ( IL 2 , TNF alpha , and IFN gamma ) , Th 2 cytokines ( IL 4 , IL 6 , and IL 10 ) , TGF beta , actin , and CD 3 delta were analyzed in acini , ducts and total tissue sections of minor salivary glands of six healthy controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NK cell and CTL activity was detected with 4 hr 51Cr release assay , cytotoxicity of macrophages was detected with indirect MTT methods , activity of IL 2 , TNF , IFN gamma were detected by routine methods . ^^^ Levels of TNF , IL 2 , IFN gamma in the supernatants of splenocytes induced in vitro were also increased . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here we report the pattern of TNF alpha , IL 1beta , IL 6 , IFN gamma , IL 1RA , and IL 4 mRNA expression , detected by RT / PCR , in the talar joint and draining popliteal lymph node ( PLN ) of rats with adjuvant arthritis ( AA ) . ^^^ Levels of TNF alpha and IFN gamma mRNA were increased in the PLN before clinical signs of arthritis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Spleen cells from sham and burn WT or lpr mice were stimulated in vitro with SEB to assess injury effects on IL 2 , TNF alpha , and IFN gamma production . ^^^ In vitro studies demonstrated that burn lpr mice produced significantly less TNF alpha , IFN gamma , IL 2 than burn WT mice ( P < . 01 , ANOVA ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Administration of bacille calmette guerin ( BCG , 15 , 30 , 50 mg / rat , i . v . ) alone or BCG with the inflammatory cytokines mixture ( CM ) , including IL 1 beta , IFN gamma , TNF alpha and lipopolysacchride ( LPS ) significantly increased NO production and LDH release in culture medium ( P < 0 . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of EC SOD was up regulated by interferon gamma ( IFN gamma ) and interleukin 4 ( IL 4 ) . and was down regulated by tumor necrosis factor alpha ( TNF alpha ) . ^^^ The Mn SOD activity was strongly up regulated by TNF alpha and IL 1alpha and moderately by IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Target cell induced IFN gamma and FcgammaRIIIA induced TNF alpha mRNA accumulation was similarly affected under the same conditions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 2 production was not up regulated , whereas both IFN gamma and TNF alpha expression significantly increased . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Combination of TNF alpha and IL 4 induced expression of TARC protein and mRNA in bronchial epithelial A 549 cells , which was slightly up regulated by IFN gamma . ^^^ The enhancement by IFN gamma was more pronounced in bronchial epithelial BEAS 2B cells , and a maximum production occurred with combination of TNF alpha , IL 4 , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine analyses revealed that T595B1 cells secreted IL 4 , IL 5 , IL 6 , and IL 10 but not IFN gamma , IL 2 , TNF , or TGFbeta , indicating that this clone belongs to the so called T helper 2 ( Th 2 ) type . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inflammatory cytokines such as tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) synergistically activate expression of the RANTES ( regulated upon activation , normal T cell expressed and secreted ) gene , which plays a crucial role in the chemoattraction of leukocytes during the inflammatory response . ^^^ Specific destruction of the IRF binding site and one of the three NF kappaB binding sites abolished the inducibility of promoter activity by IFN gamma and TNF alpha , respectively . ^^^ In electrophoretic mobility shift and immunologic assays , we showed that IRF 1 was induced after cells were treated with IFN gamma and that NF kappaB was activated by TNF alpha treatment . ^^^ These results demonstrate that both NF kappaB and IRF 1 transcription factors mediate the induction of RANTES expression via their cognate cis acting elements when cells are stimulated by TNF alpha and IFN gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study we show the capacity of an extract of the fern Polypodium leucotomos ( PLE ) to partially inhibit the production of cytokines showing a Th 1 pattern ( IL 2 , IFN gamma and TNF alpha ) in human PHA stimulated peripheral blood mononuclear cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We used defined combinations of cytokines , interferon gamma ( IFN gamma ) / interleukin 1 alpha ( IL 1 alpha ) and IFN gamma / tumor necrosis factor alpha ( TNF alpha ) , to simulate different in vitro immune environments observed in disease or inflammation . ^^^ Neither IL 1 alpha nor TNF alpha affected the IFN gamma induced antigen presentation capacity of microglia . ^^^ This shows that TNF alpha affects the of IFN gamma immunocompetent astrocytes to process and present antigen , probably either by altering membrane traffic of MHC 2 and of antigen and / or enzymatic activities associated with these mechanisms , while IL 1 alpha does so by downregulating MHC 2 expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunohistochemical staining of the brain sections demonstrated that tissue localized TNF alpha , IFN gamma , IL I1B , and IL 10 were associated with the histopathology . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This work examines the correlation between serum levels of oestrogen , progesterone and dehydroepiandrosterone sulphate ( DHEA S ) and the number of human peripheral blood cells actively secreting interleukin ( IL ) 2 , IL 4 , IL 6 , IL 10 , tumour necrosis factor alpha ( TNF alpha ) or interferon gamma ( IFN gamma ) in vivo . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , rutin and CGA augmented the inducible cytokine messages , i . e . interleukin ( IL ) 10 , IL 13 , interferon gamma ( IFN gamma ) , IL 6 and tumour necrosis factor alpha ( TNF alpha ) in IgE sensitized mast cells following antigen challenge . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Culture supernatants were analysed for interleukin 4 ( IL 4 ) , IL 5 , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) by quantitative ELISA . ^^^ The analysis showed that the synthesis of IL 4 and IL 5 but not of IFN gamma or TNF alpha was significantly higher in the nickel allergic individuals . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Bacterial endotoxins and the inflammatory cytokines TNF alpha and IFN gamma control IL 12 production by antigen presenting cells . ^^^ Utilizing IL 12 p 40 ELISPOT assays , we could confirm occurrence of elevated numbers of IL 12 p 40 secreting blood MNC after stimulation with TNF alpha , IFN gamma , LPS , LPS+TNF alpha or LPS+IFN gamma , compared to cultures without stimulant . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , HCV positive haemodialysis patients had a blunted TNF alpha response ( P < 0 . 05 ) and failed to increase the stimulated IFN gamma and IL 12 production ( P < 0 . 01 ) compared with chronic hepatitis C patients without renal disease ( n=25 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this work we have analysed the frequency of mononuclear cells spontaneously secreting the cytokines IL 2 , IL 4 , IL 5 , IL 6 , IL 10 , interferon gamma ( IFN gamma ) and tumour necrosis factor ( TNF alpha ) , in Peyer ' s patches and lamina propria of small intestine in mice by enzyme linked immunosorbent spot ( ELISPOT ) during 1 month after weaning . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine mRNA was quantified by RT PCR and revealed production of the Th 1 cytokine IFN gamma and the inflammatory cytokine TNF alpha , whereas the Th 2 cytokine IL 4 was not detected above control levels at any of the time points studied . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Ex vivo , draining lymph node cells from CBD treated mice showed a diminished CII specific proliferation and IFN gamma production , as well as a decreased release of tumor necrosis factor by knee synovial cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To elucidate how UVBR regulates iNOS expression in skin under inflammatory conditions , we investigated the effect of UVBR on NO production and iNOS expression in cultured murine keratinocyte Pam 212 cells stimulated with interferon gamma ( IFN gamma ) or tumor necrosis factor alpha ( TNF alpha ) . ^^^ Low doses of UVBR significantly suppressed IFN gamma or TNF alpha induced NO production . ^^^ UVBR also downregulated IFN gamma or TNF alpha induced iNOS expression at both the mRNA level and the protein level . ^^^ These findings suggest the possibility that the down regulatory effect of UVBR on IFN gamma or TNF alpha induced iNOS expression may , in part , explain the antiinflammatory and therapeutic properties of UVBR in inflammatory dermatoses . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In order to examine the possible involvement of cytokines in HIV 1 expression , TNF alpha , IL 1 , IL 6 and IFN gamma deficient HIV 1 transgenic mice , were produced and HIV 1 expression was analyzed after activation with bacterial lipopolysaccharides ( LPS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our results indicate that interferon gamma ( IFN gamma ) and interleukin 10 ( IL 10 ) downregulate promoter activity in endothelial cells whereas , in contrast , tumour necrosis factor ( TNF alpha ) , interleukin 1beta ( IL 1beta ) and interleukin 4 ( IL 4 ) increased the promoter activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Topical steroid treatment of allergic rhinitis decreases nasal fluid TH 2 cytokines , eosinophils , eosinophil cationic protein , and IgE but has no significant effect on IFN gamma , IL 1beta , TNF alpha , or neutrophils . ^^^ Nasal fluid levels of IFN gamma , IL 4 , IL 6 , IL 10 , IL 1beta , and TNF alpha were examined with ELISA , and IgE and eosinophil cationic protein ( ECP ) levels were examined with RIA . ^^^ By contrast , there were no significant changes of the levels of IFN gamma , IL 1beta , TNF alpha , or neutrophils . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokine profile of MBP reactive T cells that sustained the treatment was altered toward an increased production of interleukin ( IL ) 10 and decreased production of tumor necrosis factor ( TNF ) alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The inflammatory response to the presence of Ureaplasma urealyticum or Mycoplasma hominis in the lower genital tract of subfertile men without any signs or symptoms of infection was investigated by measuring the concentrations of interleukin ( IL ) 6 , IL 8 , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) in seminal plasma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Current investigations indicate that the production of TNF alpha is controlled by other mediators , including interferon gamma ( IFN gamma ) and prostaglandin E 2 ( PGE 2 ) . ^^^ In the present study , we investigated the regulatory effects of IFN gamma and / or PGE 2 on LPS induced TNF alpha production and mRNA expression in mouse peritoneal macrophages using the enzyme immunoassay and Northern blot analysis , respectively . ^^^ Exposure of macrophages to 100 U / ml of IFN gamma caused an increase in both the TNF alpha production and mRNA expression induced by LPS . ^^^ Macrophages primed with IFN gamma showed the reduced responsiveness to the suppressive effect of PGE 2 on the production of TNF alpha and the accumulation of TNF alpha mRNA . ^^^ These findings indicate that the suppressive effects induced by PGE 2 on the accumulation of TNF alpha mRNA as well as the production of TNF alpha can be reduced by the pretreatment of macrophages with IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Suppression of human IL 1beta , IL 2 , IFN gamma , and TNF alpha production by cigarette smoke extracts . ^^^ OBJECTIVE : The purpose of the current study was to evaluate the effects of cigarette smoke extracts from high tar ( unfiltered Camel ) , medium tar ( Marlboro ) , and low tar ( Carlton ) cigarettes on the in vitro production of IL 1beta , IL 2 , IFN gamma , and TNF alpha . ^^^ RESULTS : Pretreatment of PBMCs with cigarette smoke extracts derived from a single high or low tar cigarette suppressed the production of IL 1beta , IL 2 , IFN gamma , and TNF alpha by greater than 90 % without significant loss of cell viability . ^^^ Nicotine , at a concentration comparable with that found in the highest tar cigarettes ( 200 microg / mL ) , suppressed the production of IL 2 , IFN gamma , and TNF alpha by only 21 % to 38 % . ^^^ Catechol ( 50 micromol / L ) inhibited production of IL 2 and IL 1beta by 62 % to 73 % but had little effect on TNF alpha or IFN gamma production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In short term cultures , T cell receptor ( TCR ) cross linking induced further IL 4 production with little IFN gamma or TNF alpha . ^^^ In marked contrast , CD4+ T cells negative for B 7 1 expressed intracellular IFN gamma and high amounts of TNF alpha but little IL 4 upon TCR cross linking . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The influence of APC was evaluated by adding : serum levels of TNF alpha and IL 1 beta to the environment network model , and production levels of IFN gamma , IL 10 and IL 6 , after stimulus with LPS , to the cellular network model . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the cellular production of these two cytokines and of interferon gamma ( IFN gamma ) , interleukin 1alpha ( IL 1alpha ) , and tumor necrosis factor alpha ( TNF alpha ) in subjects with AN , bulimia nervosa ( BN ) , and obesity as well as in normal weight control subjects . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The mRNA expression of the cytokines IFN gamma , IL 10 and TNF alpha and the proapoptotic factor Fas ligand ( FasL ) was compared in freshly isolated CD 4 ( + ) and CD 8 ( + ) tumour infiltrating lymphocytes ( TIL ) and simultaneously obtained autologous CD 4 ( + ) and CD 8 ( + ) peripheral blood lymphocytes ( PBL ) from 20 patients with renal cell carcinomas ( RCC ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study used flow cytometry to assess the effects of recombinant IFN gamma and TNF alpha on CD 44 expression and hyaluronan binding by the rat small intestinal epithelial cell lines , RIE and IEC 6 . ^^^ IFN gamma upregulated CD 44 expression on RIE ( 155 % of unstimulated control ) and IEC 6 ( 209 % of unstimulated control ) cells , whereas TNF alpha had no effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the lung , an influx of neutrophils and elevated levels of several proinflammatory cytokines such as TNF alpha , IFN gamma , IL 6 , and IL 12 characterize this dose dependent response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) acted synergistically with TNF alpha and IL 1beta in the induction of mRNA MCP 4 mRNA expression in A 549 cells , whereas the glucocorticoid dexamethasone diminished the cytokine induced expression of MCP 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , beta catenin protein levels were reduced markedly in NTera 2 N cells by exposure to dbcAMP , EGF or bFGF , and in U 373MG cells by treatment with dbcAMP or PMA , but were unaffected in any cell lines by BDNF , TNF alpha , IL 1beta , IL 6 , IFN gamma or TGF beta 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Shortly after isolation and cell seeding , mRNA levels of monocyte derived cytokines ( IL 1alpha , IL 6 , and TNF alpha ) were significantly increased , whereas lymphocyte derived cytokines ( IL 4 , IFN gamma ) were not affected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cells were activated in vitro with either LPS , IFN gamma or zymosan and the production of following mediators was measured : reactive oxygen species using luminol dependent chemiluminescence ; nitric oxide by Griess reaction ; TNF alpha using capture ELISA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CAR bacillus specific serum immunoglobulins ( immunoglobulin M [ IgM ] , IgG 1 , IgG2a , IgG2b , IgG 3 , and IgA ) and local pulmonary cytokines ( tumor necrosis factor alpha [ TNF alpha ] , gamma interferon [ IFN gamma ] , and interleukin 4 [ IL 4 ] ) were evaluated by enzyme linked immunosorbent assay every 7 days for 49 days . ^^^ Correlating with increasing disease severity , BALB / c mice had elevations in all antibody isotypes tested , and elevations in pulmonary TNF alpha , IFN gamma , and IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In all filgrastim treated groups , tumor necrosis factor alpha ( TNF alpha ) , interleukin 12 ( IL 12 ) , and interferon gamma ( IFN gamma ) release by whole blood in response to endotoxin ( lipopolysaccharide ) was reduced . ^^^ IL 12 added in vitro to lipopolysaccharide stimulated blood of filgrastim treated donors restored IFN gamma and TNF alpha release , suggesting that the anti inflammatory effect of granulocyte colony stimulating factor is exercised through IL 12 suppression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Rapid up regulation of IFN gamma and TNF alpha gene expression , critical during bacterial infections , was markedly impaired in lung and liver tissue from gamma delta T cell deficient mice 24 h postinfection . ^^^ These data indicate that gamma delta T cells comprise a critical component of the acute inflammatory response toward extracellular Gram negative bacterial infections and are vital for the early production of the proinflammatory cytokines IFN gamma and TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Overwhelming infection in the former was associated with a pronounced decrement in the number of inflammatory cells in the lungs and elevated IFN gamma and TNF alpha levels in the lungs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Significant levels of TNF alpha , IL 1 alpha and IFN gamma were found in supernatants from cultures incubated with each of the four inducers alone . ^^^ TNF alpha , IL 1 alpha and IFN gamma levels in supernatants were significantly ( p < 0 . 05 ) higher in supernatants of cultures stimulated simultaneously with the two components than those of cultures stimulated with a single agent . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After stimulation of A 549 cells with TNF alpha , IFN gamma , or LPS , promoter activity was increased by two to threefold in the presence of adenovirus EIA . ^^^ In HBE cells , on the other hand , E1A repressed the ICAM 1 promoter after stimulation with IFN gamma and LPS with little change after TNF alpha stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Venous samples for interleukin ( IL ) 1beta , IL 2 , IL 6 , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) were taken before anaesthesia , before incision , at the end of anaesthesia and 24 h postoperatively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CD8+ T cells can produce the protective cytokines IFN gamma and TNF alpha in addition to their classical cytolytic functions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The concentrations of interleukin ( IL ) 1 , IL 4 , IL 6 , IL 10 , IL 12 , tumor necrosis factor ( TNF ) alpha , IFN gamma , IL 8 , macrophage chemoattractant protein ( MCP ) 1 , epithelial neutrophil activating peptide 78 , vascular endothelial growth factor , and thymidine phosphorylase ( TP ) were measured in 151 primary breast cancer extracts by ELISA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In these pure lymphocyte preparations the mRNA expression of IL 1 , IL 10 , IFN gamma , TNF alpha , Fas and Fas ligand was determined by using a semiquantitative RT PCR . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Fourteen to 24 mer peptides corresponding to the N and C terminal nonrepeat regions of LSA 1 stimulated production of interleukin 5 ( IL 5 ) , interleukin 10 ( IL 10 ) , gamma interferon ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) by peripheral blood mononuclear cells ( PBMC ) from 17 to 73 % of individuals in both age groups in both seasons . ^^^ Children produced less IFN gamma than adults , but IL 5 , IL 10 , and TNF alpha levels were similar for both age groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effect of CNI 1493 on the inflammatory response was studied by immunohistochemical detection of individual tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 beta ( IL 1beta ) , and gamma interferon ( IFN gamma ) producing cells in the spleen . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Identification of rat IL 1beta , IL 2 , IFN gamma and TNF alpha in activated splenocytes by intracellular immunostaining . ^^^ We have developed a sensitive three step indirect immunofluorescence method to identify individual rat cells that produce cytokines including IL 1beta , IL 2 , IFN gamma and TNF alpha . ^^^ Cells making IL 2 , IFN gamma and TNF alpha could be identified by a characteristic , intracellular , rounded , juxtanuclear immunofluorescence signal . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inhibition of the TNF alpha and IFN gamma response pathways by neutralizing monoclonal antibodies increased mycobacterial growth in whole blood . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Anti IL 6 antibody added to the M ( r ) 23 15 kDa fraction restored P 450 content to 97 % of control values , while anti IL 1beta , TNF alpha and IFN gamma antibodies had no effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We demonstrate that the level of plasma interleukin 8 ( IL 8 ) was 4 fold higher during the follicular phase than the luteal phase ( p = 0 . 004 ) , whereas plasma IL 1beta , IL 4 , IL 6 , IL 10 , interferon gamma ( IFN gamma ) , transforming growth factor beta ( TGF beta ) , tumor necrosis factor alpha ( TNF alpha ) , macrophage inflammatory protein 1alpha ( MIP 1alpha ) , and TNF receptor 2 ( TNFR 2 ) were not altered during the ovulatory cycle . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , a tendency was found towards an increased IL 1 and a reduced IL 6 , IL 8 , IFN gamma and TNF alpha expression by the BAL cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We observed that activation of 030 D cells by incubation with a supernatant derived from a Leishmania specific T cell line containing IFN gamma , TNF alpha and interleukin 2 ( IL 2 ) resulted in enhanced nitric oxide ( NO ) production by these cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma secretion as well as IFN gamma , TNF alpha , IL 4 , and IL 10 mRNA expression by lymph node MNC was down regulated in the treated rats . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In order to verify this hypothesis , cultured human myoblasts were tested for their capacity to express different pro inflammatory cytokines [ IL 1alpha , IL 1beta , IL 6 and tumor necrosis factor ( TNF ) alpha ] and chemokines ( IL 8 , MCP 1 and RANTES ) at the mRNA level and protein secretion , in the presence of the pro inflammatory cytokines IFN gamma and TNF alpha alone or in combination . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The AcOEt extract also exhibited inhibitory activity on iNOS expression in human lung epithelial A 549 cells stimulated with a combination of IFN gamma , TNF alpha and IL 1 beta without affecting the expression of constitutive isoforms of NOS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We examined if these effects were associated with a reduced renal expression of CD 54 and its ligands , interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) and MHC class 2 molecules . ^^^ Immunostaining techniques were used to follow CSS and to study the expression of CD 54 , CD 18 , CD11b / c , IFN gamma , TNF alpha and MHC class molecules . ^^^ The number of cells expressing TNF alpha , IFN gamma and MHC 2 molecules was also reduced . ^^^ This effect is associated with a reduction of cellular infiltration , IFN gamma , TNF alpha producing cells and with MHC 2 expression in the kidney . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Addition of TNF alpha , IFN gamma , IL 1beta or LPS to the medium did not result in any distinct effect on the amounts of secreted factor D . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The influence of TA on the expression of ICAM 1 and MHC 1 was studied on resting and phorbol myristate acetate ( PMA ) or interferon gamma ( IFN gamma ) and / or tumour necrosis factor alpha ( TNF alpha ) activated cells using flow cytometry and immunocytochemistry . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We compared IL 10 , IL 5 , interferon gamma ( IFN gamma ) and tumour necrosis factor ( TNF ) secretion by peripheral blood mononuclear cells ( PBMC ) isolated from eight HLA DR3+ , DQ2+ , B8+ AE patients and from 10 HLA DR 3 , DQ 2 , B 8 patients after non specific mitogenic and specific Echinococcus multilocularis antigenic in vitro stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The GA specific lines were CD3+ , CD4+ , CD 8 and produced tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , interleukin 4 ( IL 4 ) , interleukin 6 ( IL 6 ) and interleukin 10 ( IL 10 ) after stimulation with GA in the presence of irradiated peripheral blood mononuclear cells . ^^^ MBP specific T cell lines showed an identical phenotype and secreted TNF alpha , IFN gamma , IL 4 , IL 10 , but not IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The levels of the cytokines IL 1ss , TNF alpha , IL 5 , IL 10 and IFN gamma were determined in the CSF of 22 patients with active NCC , 13 patients with inactive NCC and 15 control subjects . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
FasL specific autoantibodies isolated from protected animals differentially downregulated the in vitro production of TNF alpha , but not IFN gamma , by cultured T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Four patients ( Group 1 ) showed severely depressed production of TNF alpha , IL 2 , IFN gamma , and IL 4 by CD4+ and CD8+ T cells and responded to 3 IA sessions with significant increases in CD4+TNF alpha+ , CD4+IL 2+ , and CD8+IL 2+ T cells . ^^^ Also , a tendency toward increased percentage levels of CD4+ T cells producing IFN gamma or IL 4 and of CD8+ T cells producing either TNF alpha or IFN gamma was seen , but due to the small number of patients investigated , these differences did not attain statistic significance . ^^^ Group 2 ( n = 5 ) showed unimpaired intracellular cytokine levels and responded to IA with a heterogeneous pattern of changes in TNF alpha , IL 2 , IFN gamma , and IL 4 production , but these alterations were smaller than those in Group 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Similar magnitude of inter strain differences was sustained after enhanced NO stimulation of the cells with IFN gamma + tumour necrosis factor ( TNF ) alpha , but only high ( strains BL / 10 , BL / 6 , SJL , CBA / J , C3H / HeN ) and low ( DBA / 1 , DBA / 2 , Balb / c ) NO responder phenotypes were detected after the triple cytokine cocktail composed of IFN gamma + TNF alpha + interleukin ( IL ) 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Th 1 related inflammatory cytokines like interferon gamma ( IFN gamma ) , tumour necrosis factor beta ( TNF beta ) and TNF alpha , IL 6 as well as IL 12 could play a major role in the development of tissue damage in EAN . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunohistochemical analyses of the N592 / IL 12 / IL 15 tumor area in intact nude mice revealed the presence of infiltrating macrophages , granulocytes , and NK cells , and expression of inducible NO synthase and of secondary cytokines such as IL 1beta , TNF alpha , and IFN gamma , and at higher levels GM CSF , macrophage inflammatory protein 2 , and monocyte chemoattractant protein 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma , TNF alpha , and IL 10 were diminished commensurate with the reduced cellular infiltrate ; IL 4 was not detected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In NK depleted animals the first peak of IFN gamma was absent and the second wave was slightly delayed . mRNA for IL 12 and tumour necrosis factor alpha ( TNF alpha ) as well as IFN alpha protein in serum was only recorded 24 h after infection , at the same time as the IFN gamma peak . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
BACKGROUND : In vitro production of tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , interleukin 10 ( IL 10 ) , and transforming growth factor beta ( TGF beta ) correlate with their respective genetic polymorphisms . ^^^ METHODS : Using DNA polymerase chain reaction ( PCR ) technology , polymorphisms for TNF alpha , IFN gamma , IL 10 , and TGF beta were determined for 82 kidney ( K ) and 19 simultaneous kidney pancreas ( SKP ) recipients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Human RPE cells were cultured in the presence of tumor necrosis factor alpha ( TNF alpha , 10 ng / ml ) , interferon gamma ( IFN gamma , 1000 U / ml ) or with a combination of both up to 96 hours . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The concentrations of TNF alpha , IL 1beta , IL 4 , IL 6 , IL 10 , IFN gamma , MMP 1 , and MMP 3 appeared to be similar in the two groups , although the small number of samples available precluded us from determining that there was no significant difference . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN alpha modulation of HBEC functions does not seem to be restricted to IFN gamma stimulation since either stimulatory or inhibitory effects of INF alpha on IL 8 production have been found in pilot experiments using IL 1 beta , TNF alpha , and TGF beta as stimuli . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The parallelism between serum levels of heroin and morphine ( M ) metabolites and the production of interleukin 1beta ( IL 1beta ) , interleukin 2 ( IL 2 ) , interleukin 10 ( IL 10 ) , tumor necrosis factor alpha ( TNF alpha ) , transforming growth factor beta 1 ( TGF beta 1 ) , and interferon gamma ( IFN gamma ) from murine splenocyte cultures following s . c . injection with 20 mg / kg heroin or M in C57 / BL mice is described . ^^^ Heroin and M significantly increased production of IL 1beta , IL 2 , TNF alpha and IFN gamma at 3 , 20 and 40 min from treatment , peaking at 20 min , though the effect was very brief . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cyclophosphamide treatment decreased IL 12 , IL 1beta , IL 2 , IFN gamma and TNF alpha gene expressions in mononuclear spleen cells but IL 4 gene expression increased . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
BA did not interact with CX3C chemokine fractalkine / neurotactin or other cytokines , such as TNF alpha and IFN gamma , indicating that its action is selective . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In in vitro experiments using human peripheral blood mononuclear cells ( PBMC ) , OK PSA induced IFN gamma , interleukin ( IL ) 2 , IL 12 , IL 18 , tumor necrosis factor ( TNF ) alpha and TNF beta that are generally called `` Th 1 type cytokines ' ' both in protein and in mRNA levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Formation of antigenic peptides by the multicatalytic proteinase complex ( MPC , proteasome ) is facilitated by incorporation of three subunits ( LMP 2 , LMP 7 and LMP 10 ) that are inducible by IFN gamma and TNF alpha . ^^^ To learn more about the relationship between control of cellular immunity and expression of LMP subunits , we measured LMP 7 levels in human umbilical vein endothelial cells of cytokines promoting cellular immunity ( IL 12 , IFN gamma , TNF alpha ) or humoral immunity ( IL 10 , IL 6 ) . ^^^ IFN gamma upregulated LMP 7 levels , as did TNF alpha to a lesser extent . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Exposure of the cells to LPS , IL 1 , IFN gamma and TNF alpha produced a reduction of C / EBP alpha mRNA levels and a corresponding increase in the expression of C / EBP beta and C / EBP delta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In in vitro model using human peripheral blood mononuclear cells ( PBMC ) , the 55 kDa protein ( AILb A ) induced multiple cytokines , such as IFN gamma , tumor necrosis factor ( TNF ) alpha , granulocyte macrophage colony stimulating factor ( GM CSF ) , IL 2 , IL 6 , IL 10 , IL 12 and IL 18 , and also accelerated killer cell activities of PBMC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of inducible nitric oxide synthase ( iNOS ) protein was studied in the brain after intracerebroventricular injections of interferon ( IFN ) gamma , and IFN gamma combined with lipopolysaccharide ( LPS ) or tumor necrosis factor ( TNF ) alpha , compared to ovalbumin as control . ^^^ No immunopositive cells were seen in the brain of IFN gamma receptor knockout mice after IFN gamma administration ; very few immunostained macrophages were detected in these cases , mostly around the injection needle track , after co administration of LPS or TNF alpha . ^^^ The data also demonstrate that the signaling cascade activated by IFN gamma binding to its receptor is critical for iNOS induction , and the synergistic action of LPS and TNF alpha as iNOS inducers in brain cells is largely mediated by the receptor regulated action of IFN gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Differently , Sb 26 infection failed to alter CNS structure , while inducing IL 12 p 40 , TNF alpha , IL 1beta , IFN gamma and iNOS specific gene expression as well as IL 12 , TNF alpha and IL 1beta cytokine production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This stability may be greatly influenced by pro inflammatory mediators such as IFN gamma , TNF alpha , and Il 1beta and Fas ligand ( FasL ) that are present in human atheroma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of interferon gamma ( IFN gamma ) , interleukins 10 ( IL 10 ) and 13 ( IL 13 ) , transforming growth factor beta ( 1 ) ( TGF beta ( 1 ) ) and tumor necrosis factor alpha ( TNF alpha ) on the DNA binding proteins were studied by electrophoretic mobility shift assay ( EMSA ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Microsatellites associated with cytokine production have been detected in the first intron of the IFN gamma gene and flanking the TNF alpha gene . ^^^ A PCR method using sequence specific primers ( SSP ) was developed for genotyping the IL 2 polymorphism , and the ABI PRISMtrade mark 310 genetic analyser was used to detect the TNF alpha and IFN gamma microsatellites . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , there is a marked polarization in cytokine production from lymphocytes harvested from the transplanted animals , with preferential production of IL 4 , IL 10 , and TGFbeta on donor specific restimulation in vitro , and decreased production of IL 2 , IFN gamma , and TNFalpha compared with non pv immunized control transplanted mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha alone neither induced iNOS in USAC cells nor caused production of NO , but addition of TNF alpha to USAC cells pretreated with LPS and IFN gamma enhanced the expression of iNOS mRNA , induced iNOS protein and produced NO . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The disease modulatory effects of IL 12 and anti IL 12 mAb were associated with profound and reverse modifications of a ConA induced increase in the circulating levels of IL 4 , IL 6 , interferon gamma ( IFN gamma ) and tumor necrosis factor ( TNF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of hepatocytes with interleukin 1beta ( IL 1beta ) plus interferon gamma ( IFN gamma ) , which induced iNOS expression and NO production , suppressed TNF alpha / ActD induced Bid cleavage and mitochondrial cytochrome c release . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 2 activated killer lymphocytes ( LAK cells ) secrete inflammatory cytokines such as interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNFalpha ) that can induce nitric oxide ( NO ) synthesis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
R 848 and imiquimod belong to a class of immune response modifiers that are potent inducers of cytokines , including IFN alpha , TNF alpha , IL 12 , and IFN gamma . ^^^ The mechanism of R 848 ' s adjuvant activity is linked to cytokine production , where increases in IgG2a levels are associated with IFN alpha , TNF alpha , IL 12 , and IFN gamma induction , and decreases in IgE levels are associated with IFN alpha and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both IFN gamma dependent and independent cytotoxicity were partially blocked either by anti tumor necrosis factor alpha ( TNF alpha ) antibody or by the inhibitor of nitric oxide synthesis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We therefore investigated the in vitro IL 17 response to a variety of mitogens and antigens , and compared the IL 17 response to interferon gamma ( IFN gamma ) , IL 4 , IL 10 and TNF alpha . ^^^ IL 17 production did not correlate with either the type 1 cytokine IFN gamma or TNF alpha or the type 2 cytokine IL 4 or IL 10 at either the mRNA or protein level . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The concentrations of IL 1beta , IL 2 , IL 4 , IL 5 , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) were measured serially in the lungs of mice with experimentally induced chronic respiratory P . aeruginosa infection until 60 days after inoculation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To investigate the effect of cytokines on the interaction of human umbilical vein endothelial cells ( HUVEC ) with Pseudomonas aeruginosa , cells were treated with interferon gamma ( IFN gamma ) plus tumour necrosis factor alpha ( TNF alpha ) for 24 hr and exposed to P . aeruginosa suspension for 1 hr . ^^^ Our results indicate that HUVEC can be activated by IFN gamma plus TNF alpha to kill IC P . aeruginosa and suggest a role for reactive oxygen radicals , notably hydrogen peroxide , in HUVEC antibacterial activity . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In order to investigate the role of type 1 and type 2 cytokines in the remission of Graves ' disease ( GD ) during pregnancy , spontaneous and mitogen stimulated production of interleukin ( IL ) 4 , IL 6 , IL 10 , IL 12 , interferon gamma ( IFN gamma ) , and tumour necrosis factor alpha ( TNF alpha ) were measured by enzyme linked immunospot assay of peripheral blood mononuclear cells from 10 pregnant women with GD , 8 healthy pregnant women , and 10 healthy nonpregnant women . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha , IFN gamma and IL 12 serum levels were higher at earlier time points in monoassociated mice when compared with germ free mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To investigate the role of interferon ( IFN ) gamma and tumor necrosis factor ( TNF ) alpha and their potential to replace each other in the process of fetal porcine islet like cell cluster ( ICC ) xenograft rejection , mice with a targeted disruption of the IFN gamma receptor gene and wild type controls were transplanted with fetal porcine ICCs under the kidney capsule and given post transplant treatment with the TNF alpha inhibiting agent MDL 201 , 449A . ^^^ Taken together , findings in this study indicate that , in the pig to mouse model , IFN gamma , TNF alpha , and interleukin 2 seem to be of importance to fetal porcine ICC xenograft rejection . ^^^ Nevertheless , in a majority of animals , other cytokines eventually substitute for the lack of IFN gamma , TNF alpha and interleukin 2 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha and IFN gamma production returned to pre aspirin levels one month after the discontinuation of aspirin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , interleukin ( IL ) 6 , IL 10 , IL 12 , interferon ( IFN ) gamma , and tumor necrosis factor ( TNF ) alpha and beta mRNA were elevated in PBMC and cervical mucosa biopsy specimens of ESN and HIV infected women ; CCR 5 and CXCR 4 mRNA were augmented in cervical mucosa biopsy specimens , but not in PBMC , of ESN and HIV infected women ; HIV specific IFN gamma secreting cells were detected in vaginal washes of ESN and HIV infected women ; and phenotypic alterations were present in PBMC of ESN women . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
LPS stimulated up regulation of CXCR 4 and CCR 5 in vitro was inhibited by anti TNF and anti IFN gamma . ^^^ Incubation with recombinant TNF or IFN gamma mimicked the LPS effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present work the regulatory effect of IL 1beta , IL 6 , TNF alpha , IFN gamma and TGF beta 1 on syndecan 1 and syndecan 2 expression of hepatocytes , hepatoma cell lines , liver and skin fibroblasts has been studied . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of AdIkappaB transfected cells with cytomix [ 1000 U / mL IFN gamma , 1 nM IL 1beta , and 10 ng / mL tumor necrosis factor alpha ( TNFalpha ) ] or TNFalpha containing cytokine combinations resulted in inhibition of cytokine induced nitrite production and a marked increase in apoptosis compared to control cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , the effect of pro inflammatory cytokines , such as interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) , was investigated in biopsy specimens of normal skin and BCC , using a biopsy culture system and immunohistochemistry . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , stimulation with tumour necrosis factors ( TNF alpha ) or interferon gamma ( IFN gamma ) induced MCP 1 mRNA and protein synthesis in both cell types . ^^^ Together , TNF alpha and IFN gamma acted synergistically to increase MCP 1 production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CD 2 induced proliferation as well as production of TNF alpha and IFN gamma was abrogated in ZAP 70 deficient T cells , whereas PMA plus ionomycin induced normal activation of these cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The present study investigates the regulatory mechanisms involved in the cooperation between IFN gamma and TNF alpha to promote transcription from IFN regulatory factor 1 ( IRF 1 ) . ^^^ A subsequent DNase 1 footprint analysis showed that the region between 171 and 144 was inducibly protected with stimulation by TNF alpha , and this protection was significantly enhanced with the combination of IFN gamma and TNF alpha . ^^^ In an EMSA with the protected region as a probe , a TNF alpha inducible complex ( C 1 ) and an IFN gamma inducible complex ( C 2 ) , but no synergy specific DNA protein complexes , were recognized . ^^^ Because the base substitutions over the pdkappaB region ( from 171 to 144 ) affected not only the TNF alpha response but also that of IFN gamma , this region might contribute to the cooperative action of the NF kappaB subunits with the IRF 1 related factor . ^^^ Finally , we demonstrated that none of the cis acting elements , ppkappaB , pdkappaB , or IFN gamma activation site / kappaB , is dispensable for the optimal synergism in response to IFN gamma and TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 18 administration to IL 12 ( / ) mice increased glomerular ICAM 1 protein expression , but did not restore Ag stimulated splenocyte IFN gamma , GM CSF , IL 2 , or TNF alpha production . ^^^ These studies demonstrate that even in the absence of IL 12 , IL 18 can induce in vivo DTH responses and up regulate ICAM 1 without inducing IFN gamma , GM CSF , or TNF alpha production . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Likely candidates for this process would include the release of tumour necrosis factor alpha ( TNF alpha ) from alveolar macrophages stimulated by interferon gamma ( IFN gamma ) released from NK cells that are reconstituted early after BMT . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These mechanisms are regulated by a number of cytokines , including IL 2 , IL 4 , IL 5 , IFN gamma , and TNF alpha , which follow the classical T ( H ) 1 / T ( H ) 2 immunologic paradigm . ^^^ RESULTS : There was a transient polarized pattern corresponding to a T ( H ) 1 response with IL 2 , IFN gamma , and TNF alpha in nonimmediate reactions and to a T ( H ) 2 response with IL 4 in immediate reactions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TDM may interact specifically with macrophages in vivo , as evidenced by induction of TNF alpha , IL 1beta , and IL 6 , but not IFN gamma , protein in bone marrow derived macrophages from C57BL / 6 mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The absence of host derived IL 12 , IFN gamma , or the TNF receptor 1 or receptor 2 gene did not attenuate tumor growth or improve subsequent cachexia . ^^^ Thus , carcass weight loss was not improved by the omission of host cytokine ( TNF alpha , IL 12 , or IFN gamma ) except for IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This DSG treatment reduced hsp 70 levels in spleen and lymph nodes , inhibited anti hsp 70 antibody production , and diminished the serum levels of IL 2 , IFN gamma , TNF alpha , and IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effect of gamma interferon ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and lipopolysaccharide ( LPS ) on nitric oxide ( NO ) production in the mouse vascular aortic endothelial cell line END D was examined . ^^^ LPS , TNF alpha , and a low concentration of IFN gamma inhibited NO production in END D cells , while a high concentration of IFN gamma definitely enhanced it . ^^^ The NO production induced by a high concentration of IFN gamma was further augmented by using IFN gamma in combination with LPS or TNF alpha . ^^^ The augmentation of NO production by IFN gamma alone or in combination with LPS or TNF alpha was completely blocked by several inhibitors of iNOS . ^^^ LPS and TNF alpha exclusively modulated the activity of iNOS once its expression was triggered by IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro TNF 70 80 and recombinant human TNF ( hTNF ) acted with interferon gamma ( IFN gamma ) to reduce bacterial replication and to induce synthesis of bactericidal nitric oxide ( NO ) in BCG infected , bone marrow derived murine macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , in differentiated myotubes , TNF plus interferon gamma ( IFN gamma ) signaling was required for NF kappaB dependent down regulation of MyoD and dysfunction of skeletal myofibers . ^^^ MyoD mRNA was also down regulated by TNF and IFN gamma expression in mouse muscle in vivo . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine analysis during the period of T cell activation in HLH revealed marked elevation of interferon ( IFN ) gamma , interleukin ( IL ) 10 and soluble IL 2 receptor ( sIL 2R ) and mild to moderate increases of tumor necrosis factor ( TNF ) alpha were observed , while IFN gamma , IL 10 and sIL 2R were elevated initially during the HLH phase , which then decreased as LPD developed and B cell proliferation predominated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To investigate the mechanism how Am 80 alone delayed the response , the expressional levels of mRNA for interleukin 6 ( IL 6 ) , interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) in spinal cord were examined . ^^^ Transcriptional levels of IL 6 , IFN gamma and TNF alpha were parallel with the clinical symptoms of the disease in Am 80 treated rats , that is , expressional levels of their mRNA were diminished during the administration of Am 80 , which then increased as soon as the administration was stopped . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A selective elevation in the serum TNF alpha levels was observed at the initial phase of the fever due to S . cerevisiae mannan , whereas there was no significant change on the serum levels of TNF alpha , IL 1beta and IFN gamma during the latent period or at the initial phase of the fever induced by C . albicans mannan . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The levels of IFN gamma , TNF alpha and IL 10 mRNA were also higher in CI . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increased survival of C57BL / 6 renal allografts following portal vein donor specific pretransplant immunization of C3H mice is associated with increased expression of the molecule OX 2 seen on host dendritic cells , along with a marked polarization in cytokine production from lymphocytes harvested from the transplanted animals , with preferential production of IL 4 , IL 10 , and TGF beta on donor specific restimulation in vitro , and decreased production of IL 2 , IFN gamma , and TNF alpha compared with non portal vein immunized control transplanted mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TGF beta 1 and IFN gamma direct macrophage activation by TNF alpha to osteoclastic or cytocidal phenotype . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1 , TNF alpha , and LPS blocked the activation of Stat DNA binding and tyrosine phosphorylation by IL 6 and IL 10 , but not by IFN gamma , in primary macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our results demonstrated that exposing LPS stimulated human monocytes to NCX 4016 resulted in a 40 80 % inhibition of IL 1beta , IL 8 , IL 12 , IL 18 , IFN gamma , and TNF alpha release with an EC ( 50 ) of 10 20 microM for IL 1beta and IL 18 . ^^^ IL 18 immunoneutralization resulted in a 60 80 % reduction of IL 1beta , IL 8 , IFN gamma , and TNF alpha release from LPS stimulated monocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Measurements of macrophage activation ( neopterin ) , nitric oxide production ( nitrite ) , and tumor necrosis factor alpha ( TNF alpha ) , IL 1alpha , IL 1beta , IFN gamma , IL 6 , IL 10 , and soluble IL 2 receptor ( sIL 2R ) were performed . ^^^ In addition , an unusual cytokine elaboration composed of IL 6 , IFN gamma , IL 10 , nitrite , neopterin , and sIL 2R , but not the expected TNF alpha and IL 1 , was detected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The response to rhIL 10 was assessed by inhibition of tumor necrosis factor alpha ( TNF alpha ) , IL 6 , and interferon gamma ( IFN gamma ) production . ^^^ RhIL 10 inhibited TNF alpha , IL 6 , and IFN gamma synthesis in PBMC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The surface antigen was found to be relatively unaffected by cytokines , but the release of soluble LFA 3 ( sLFA 3 ) was highly responsive to interleukin 1beta ( IL 1beta ) , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
I / CB PE exposed T cells were evaluated for mRNA content of IFN gamma , IL 4 , TNF alpha , TGF beta 1 , TGF beta 2 , and IL 10 , and their supernatants were analyzed for content of TGF beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The human monocytic cell line THP 1 differentiates along the macrophage line after phorbol 12 myristate 13 acetate ( PMA ) supplementation and can be stimulated to secrete tumour necrosis factor alpha ( TNF alpha ) by interferon gamma ( IFN gamma ) addition . ^^^ In addition , we found that iron administration to PMA differentiating cells induced the expression of TNF alpha mRNA and TNF alpha secretion to levels even higher than those induced by IFN gamma alone . ^^^ In contrast , preincubation of the cells with iron before PMA induction resulted in a decrease of the TNF alpha secretion induced by IFN gamma , whereas the opposite was true after preincubation with desferrioxamine . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we examined the effects of IL 12 on expression of several cytokines , including IFN gamma , IL 2 , tumor necrosis factor alpha ( TNF alpha ) , and IL 10 , by primary human CD 3 ( + ) T cells . ^^^ Restimulation of T lymphoblasts on immobilized anti CD 3 monoclonal antibodies ( mAb ) induced rapid expression of TNF alpha mRNA and more gradual increases in mRNA levels for IL 2 , IFN gamma , and IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 10 , IL 13 , IFN gamma , tumor necrosis factor ( TNF ) alpha , LT alpha , CD 154 , and TNF related activation induced cytokine ( TRANCE ) were expressed by 2 20 % of rheumatoid arthritis ( RA ) synovial tissue CD 4 ( + ) memory T cells , whereas CD 4 ( + ) cells that produced IL 2 , IL 4 , or IL 6 were not detected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We did this by testing the action of MGN 3 on the levels of both tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) secretions and MGN 3 function on the expression of key cell surface receptors . ^^^ MGN 3 also increased production of IFN gamma but at lower levels as compared to TNF alpha With respect to key cell surface receptors , MGN 3 increases the expression of CD 69 , an early activation antigen at 16 hours after treatment . ^^^ Treating highly purified NK cells with MGN 3 also resulted in increased levels of TNF alpha and IFN gamma secretion in conjunction with augmentation of NK cell cytotoxic function . ^^^ Furthermore , addition of MGN 3 to interleukin 2 activated NK cells resulted in a synergistic induction of TNF alpha and IFN gamma secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The production of IL 2 , TNF beta , IFN gamma and IL 4 was measured in the supernatants of the cultures by ELISA . ^^^ In situ hybridization for mRNA was performed using oligonucleotide probes for IL 4 , IFN gamma and TNF beta in cell smears . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , IFN gamma induced a marked increase in Tf synthesis by macrophages , while IL 1 , IL 6 and TNF alpha produced a more modest increase . ^^^ IFN gamma alone and in combination with other cytokines was effective in inducing nitrite ( NO ) production and inducible nitric oxide synthetase ( iNOS ) expression in macrophages , while IL 1 , TNF alpha and IL 6 individually , as well as in various combinations , were not . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Donor and recipient cornea was examined by quantitative competitive reverse transcription polymerase chain reaction ( RT PCR ) for hypoxyanthine phosphoribosyltransferase ( HPRT ) , CD 3 , CD 25 , interleukin ( IL ) 1beta , IL 1RA , IL 2 , IL 6 , IL 10 , interferon gamma ( IFN gamma ) , tumor necrosis factor ( TNF ) , transforming growth factor ( TGF ) beta 1 , and macrophage inflammatory protein ( MIP ) 2 and by nonquantitative RT PCR for IL 4 , IL 5 , IL 12 p 40 , IL 13 , TGF beta 2 , monocyte chemotactic protein 1 ( MCP 1 ) , MIP 1alpha , MIP 1beta , and RANTES ( for regulated upon activation normal T cell expressed and secreted ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , the levels of tumor necrosis factor alpha , IFN gamma , IL 6 , IL 12 , and KL 6 ( a mucin like glycoprotein expressed on type 2 pneumocytes ) were measured in selected samples . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Frequencies of IL 2 , TNF alpha , IFN gamma , and IL 10 expressing memory responders increased over their primary response counterparts , but were still markedly lower than revealed in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytotoxic activity of IFN gamma stimulated monocytes against human squamous carcinoma cell line HSC 3 was inhibited partially by anti TWEAK mAb alone and almost completely by combination with anti TRAIL ( TNF related apoptosis inducing ligand ) mAb . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although they are known for their capacity to kill infected cells , Ag specific CD 8 ( + ) T cells elaborate other effector mechanisms , including TNF and IFN gamma , that contribute to defense against infection . ^^^ In this study , we show , using in vitro propagated and directly ex vivo analyzed Ag specific CD 8 ( + ) T cells , that in contrast to Ag dependent ON / OFF cycling of IFN gamma production , the cessation of TNF production by the same IFN gamma producing cells is rapid and Ag independent . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
LT and LTR 72 suppressed LPS and IFN gamma induced TNF alpha and IL 12 production , but enhanced IL 10 secretion by macrophages in vitro and suppressed IL 12 production in vivo in a murine model of LPS induced shock . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of a mouse macrophage cell line with LPS , synthetic lipid A , IL 2 , IL 15 , IL 1beta , IFN gamma , or TNF alpha significantly increased TLR 2 mRNA expression , whereas TLR 4 mRNA expression remained constant . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
T cells stimulated by tetanus toxoid pulsed , IS PL matured MODC were significantly more frequently IFN gamma positive ( 25 . 2+ / 2 . 7 % ) as compared to TNF alpha treated MODC ( 15 . 4+ / 1 . 4 % ) , indicating a strong activation of Th 1 lymphocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The in vitro experiments demonstrated that treatment with IL 1 beta and TNF alpha resulted in 2 . 6 fold and 4 fold stimulation of elafin secretion , respectively , whereas IL 6 , neutrophil elastase and IFN gamma caused no significant changes in elafin release . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The pro inflammatory cytokines IFN gamma and TNF alpha are believed to be important modulators of multiple sclerosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Product R ( Reticulose ( TM ) ) is a peptide nucleic acid immunomodulator with broad spectrum antiviral activity that was recently shown to increase expression of mRNAs encoding the proinflammatory cytokines , IFN gamma , IL 1beta , IL 6 and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The results show that TNF alpha production was somewhat elevated in active MS with no significant increase in the level IFN gamma , whereas in the chronic phase the anti inflammatory cytokines IL 10 and TGF beta increased , accompanied by a reduction in IFN gamma when stimulated by myelin basic protein . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OBJECTIVES : To study the effects of intradermally injected TNF alpha , interleukin ( IL ) 1beta and IFN gamma on KC apoptosis in the back skin of C57BL / 6 mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Poxviruses encode a broad range of proteins that interfere with host immune functions , such as soluble versions of receptors for the cytokines tumor necrosis factor , interleukin 1 beta , gamma interferon ( IFN gamma ) , IFN alpha / beta , and chemokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Progressive upregulation in the transcripts for Th 1 cytokines ( IL 12 , IFN gamma , IL 1 , TNF alpha ) was observed with increasing dosage of DSS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The serum soluble FasL ( sFasL ) concentration of this patient was very high , whereas the serum levels of tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , interleukin 1 beta ( IL 1beta ) , interleukin 2 ( IL 2 ) and thrombopoietin were normal . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the previous study , we reported that suppression of EAN by Rolipram was associated with down regulated myelin antigen induced T cell responses as well as downregulated IFN gamma and TNF alpha production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , PMN stimulated by OMV in the presence of IFN gamma demonstrated an enhanced capacity to release TNF alpha , IL 1beta , IL 8 , and MIP 1beta compared to stimulation with OMV alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Upon infection , compared to C57BL / 6 mice , BALB / c mice developed an earlier response of interleukin 12 ( IL 12 ) , gamma interferon ( IFN gamma ) , tumor necrosis factor alpha , and macrophage chemoattractive protein 1 , and greater neutrophilic influx to the lung by days 7 and 14 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Several cytokines , including TNF alpha , IL 1alpha , and GM CSF , but not IFN gamma , induced TLR 2 mRNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These CD 8 ( + ) T cells produced IFN gamma and TNF alpha and recognized Ag pulsed or bacillus Calmette Gurin infected , HLA A * 0201 positive , but not HLA A * 0201 negative or uninfected human macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The delay in neonates corresponded to delayed kinetics of expression of lung cytokines TNF alpha and IFN gamma mRNA and chemokines lymphotactin , RANTES , and macrophage inflammatory protein 1ss mRNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In fact , the activation of PMNLs with TNF alpha and IFN gamma induced the expression of CD 83 , a dominant cell surface marker of DCs . ^^^ When PMNLs were activated with granulocyte macrophage colony stimulating factor , TNF alpha , and IFN gamma , these cells expressed CD 40 and HLA DR in addition to CD 83 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
On day 14 after SZ , fusidin markedly altered the circulating cytokine profile induced in vivo by ConA , reducing the levels of IFN gamma , IL 2 and TNF alpha and augmenting the level of IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We therefore investigated the expression of IL 2 , IL 4 , IL 13 , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) intracellularly in bronchoalveolar lavage ( BAL ) and peripheral blood CD3+ T lymphocytes from patients with pulmonary sarcoidosis ( radiologic stage 2 3 , n = 8 ) and normal controls ( n = 9 ) by flow cytometry . ^^^ In contrast to IL 4 and IL 13 , the percentage of T lymphocytes expressing intracellular IL 2 ( 49 . 3 + / 21 . 3 % versus 14 . 5 + / 15 . 6 % ) , IFN gamma ( 75 . 5 + / 14 . 9 % versus 32 . 6 + / 18 . 7 % ) and TNF alpha ( 68 . 3 + / 18 . 7 % versus 36 . 8 + / 20 . 8 % ) was significantly higher in patients with sarcoidosis than in normal controls ( each P < 0 . 005 ) . ^^^ Close correlations were observed between the percentages of BAL lymphocytes expressing intracellular IL 2 , IFN gamma and TNF alpha , but not for IL 4 or IL 13 . ^^^ Analysis of the expression of these cytokines in T lymphocyte subsets revealed IL 2 , IFN gamma , and TNF alpha in CD4+ as well as CD8+ T lymphocytes , suggesting a contribution of TC 1 cells to the production of proinflammatory cytokines in sarcoidosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
While peak serum TNFalpha concentrations were inversely related to baseline corticosterone concentrations , there was no correlation between peak IFN gamma concentrations and baseline corticosterone concentrations or between TNFalpha and IFN gamma concentrations . ^^^ We conclude that surgical stress associated with elevated concentrations of endogenous corticosterone does not attenuate the endotoxin induced IFN gamma response despite an attenuation of the endotoxin induced TNFalpha response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Beta cell destruction has been shown to occur when rodent or human islets are exposed in vitro to inflammatory cytokines , such as interleukin 1beta ( IL 1beta ) , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Disks of HDSC were predegraded with collagenase or impregnated with tumor necrosis factor alpha ( TNF alpha ) or interferon gamma ( IFN gamma ) before implantation in 129 SVEV mice . ^^^ This suggests that lack of proteolytic enzymes or TNF alpha or IFN gamma is not the cause of the impaired onset of the foreign body reaction . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A Th 1 response characterized by the presence of IFN gamma and TNF alpha in serum was observed during early infection , with significantly higher levels in the non depleted animals . ^^^ Our results suggest that PMNs have little influence on the control of C . abortus secondary infection , although they are a first line of defense and may influence the early production of TNF alpha and IFN gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the presence of interleukin ( IL ) 2 , interferon gamma ( IFN gamma ) , IL 4 , tumor necrosis factor alpha , ( TNF alpha ) , and IL 1beta messenger RNA ( mRNA ) in 8 biopsy specimens of DLE and compared it with 3 biopsy specimens of normal skin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Mice ( BALB / c wild type ( wt ) , MRL lpr / lpr , BALB / c interferon gamma knock out ( IFN gamma KO ) , C57BL / 6 inducible nitric oxide synthase ( iNOS ) KO , and BALB / c tumour necrosis factor alpha ( TNF alpha ) KO ) were injected intraperitoneally each day with IL 12 ( 20 ng / g / mouse ) and / or IL 18 ( 200 ng / g / mouse ) . ^^^ CONCLUSIONS : Our study strongly indicates that IL 18 together with IL 12 induces intestinal mucosal inflammation in an IFN gamma dependent but TNF alpha , NO , and Fas ligand independent manner , and fatty liver is dependent on IFN gamma and NO . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : We have compared the production of IL 4 , IL 6 , IFN gamma , and TNF alpha from peripheral blood leukocytes between atopic ( n=21 ) and nonatopic ( n=22 ) asthmatics and healthy nonatopic subjects ( n=20 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma treated RAW 264 . 7 cells secreted proinflammatory cytokines , including tumor necrosis factor ( TNF ) alpha and interleukin ( IL ) 1beta . ^^^ The addition of IFN gamma treated RAW 264 . 7 cell conditioned media to P+ subclones led to a significant enhancement of NO 2 formation that was diminished by the TNF alpha specific but not IL 1beta specific antibody . ^^^ When combined with IFN gamma , the recombinant TNF alpha ( 1 100 ng / ml ) enhanced NO 2 formation in JB 6 P+ cells , whereas IL 1beta ( 1 100 ng / ml ) did not . ^^^ These results led us to conclude that IFN gamma treated RAW 264 . 7 cells release TNF alpha to induce iNOS expression in promoter sensitive JB 6 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
KGF treated recipients had elevated serum levels of the Th 2 cytokine interleukin 13 ( IL 13 ) on day 6 after T cell transfer concomitant with reduced levels of the inflammatory cytokines tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The patterns of cytokine induction in the CD 25 depleted and control cultures were similar with increases of IFN gamma , GM CSF , IL 16 , TNF alpha , and soluble IL 2 receptor in the IL 2 containing cultures . ^^^ IL 2 as a sole stimulant is also associated with generation of a cytokine milieu that includes IFN gamma , GM CSF , IL 16 , and TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The 5 ; flanking region of the rat iNOS gene contains interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) responsive elements and an NF kappaB binding consensus sequences . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found that endotoxin free methanol extracts from PM leaves , at doses of 50 , 100 , 250 , and 500 microg / mL , were associated with 4 . 4 + / 1 , 6 + / 1 , 12 + / 0 . 4 , and 18 + / 0 . 4 fold increases of nitric oxide ( NO ) production , and increased TNF alpha production ( 621 + / 31 , 721 + / 36 , 727 + / 36 , and 1056 + / 52 U / mL , respectively ) by rat peritoneal macrophages , in the absence of IFN gamma or LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To explore the role of cytokines in Ag specific immunotherapy , we analyzed mRNA induction and protein expression for the proinflammatory cytokines TNF alpha and IFN gamma , the anti inflammatory cytokine IL 10 , and the cytokine inducible NO synthase ( iNOS ) during high dose Ag therapy of adoptive transfer experimental autoimmune encephalomyelitis ( AT EAE ) in the Lewis rat . ^^^ Using semiquantitative and competitive RT PCR , we found 5 to 6 fold induction of TNF alpha mRNA and 3 fold induction of IFN gamma mRNA in the spinal cord that occurred within 1 h after i . v . injection of Ag and was accompanied by a 2 fold increase of iNOS mRNA . ^^^ Both IFN gamma and iNOS mRNA remained elevated for at least 6 h , whereas TNF alpha mRNA was already down regulated 6 h after Ag injection . ^^^ A comparable time course was found for circulating serum levels of TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the absence of fluconazole , the addition of tumor necrosis factor alpha ( TNF alpha ) , interleukin 1beta ( IL 1beta ) , gamma interferon ( IFN gamma ) , or IL 4 had no effect on the growth of C . albicans . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These effector cells produced 12 times more IFN gamma , two times more TNF alpha , and three times less IL 4 than controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
During early acute disease , we have found a strong proinflammatory ( Th 1 ) cytokine response in the CNS of both TMEV infected SJL and B 6 mice , demonstrated by the expression of transcripts for IFN gamma , IL 1 , IL 6 , IL 12p40 , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Vector administration also induced a potent inflammatory response characterized by complement activation and the induction of the cytokines IFN gamma , TNF alpha , IL 6 , and IL 12 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
GM CSF may also regulate IFN gamma and TNF alpha production and activity in response to infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha and IFN gamma production were modified in ACD ; TNF alpha in both genders and IFN gamma in female mice only . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using a two separate chambers system , we showed that Brucella fraction , as well as isopentenyl pyrophosphate activated Vgamma9Vdelta2 T cells , impaired the multiplication of B . suis in differentiated THP 1 cells through TNF alpha and IFN gamma release . ^^^ In the present study , using circulating Vgamma9Vdelta2 T cells and autologous monocytes infected with B . suis , we provide evidence that 1 ) intramonocytic multiplication of B . suis is impaired by supernatants of activated Vgamma9Vdelta2 T cells in part via TNF alpha and IFN gamma , this impairment occurring without host cell lysis ; 2 ) unstimulated Vgamma9Vdelta2 T cells can impair intracellular bacterial multiplication after their activation by soluble factors released by infected monocytes ; and 3 ) activated Vgamma9Vdelta2 T cells lyse Brucella infected monocytes in a contact dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Given the influence of IL 12 in T cell differentiation , as well as the roles of transforming growth factor beta ( TGF beta ) , nitric oxide and tumour necrosis factor alpha ( TNF alpha ) in the immune response against intracellular pathogens , we decided to analyse the interferon gamma ( IFN gamma ) , IL 4 , IL 12 , TGF beta , TNF alpha and nitrite concentrations in culture supernatants of PBMC from TB patients showing different degrees of lung involvement . ^^^ Antigen stimulated IFN gamma , TGF beta and TNF alpha production was found to be significantly increased in TB patients , both moderate and advanced , compared with the controls . ^^^ Levels of IFN gamma were significantly higher in moderate disease than advanced cases , whereas advanced cases showed significantly higher IL 12 , TGF beta and TNF alpha concentrations when compared with cases of moderate TB . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Apoptosis induction could be abrogated mainly by antibodies against tumour necrosis factor alpha ( TNF alpha ) and , to a lesser extent , by antibodies against IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Abs to IL 1 alpha , IL 1 beta , IL 2 , IL 3 , IL 10 , IL 15 , GM CSF , IFN gamma , and TNF alpha were tested for their effects on anti CD 3 mAb , Con A , and PMA / ionomycin mediated monocyte HA binding in PBMC cultures . ^^^ Anti TNF alpha , anti IL 2 , and anti IFN gamma Abs , when added together to PBMC cultures , completely blocked Con A and partially blocked anti CD 3 and PMA / ionomycin induced monocyte HA binding . ^^^ Likewise , IFN gamma augmented TNF alpha induced monocyte HA binding . ^^^ Moreover , anti IFN gamma and anti TNF alpha Abs blocked fixed PMA / ionomycin activated CD 4 ( + ) T cell induced monocyte HA binding . ^^^ Taken together , these studies indicate roles for soluble T lymphocyte derived factor ( s ) , such as IL 2 and IFN gamma , and a role for monocyte derived TNF alpha in Con A , TCR complex , and PMA / ionomycin induced HA binding to monocyte CD44 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Enhanced secretion of IFN gamma by activated Th 1 cells occurs via reverse signaling through TNF related activation induced cytokine . ^^^ Thus , in addition to its role in activating dendritic cells by binding to the receptor RANK , TRANCE itself can signal the augmentation of IFN gamma secretion via a p 38 dependent pathway , and this provides yet another example of reverse signaling by a member of TNF superfamily . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 18 ( / ) mice also had significantly reduced Ag specific proliferation and proinflammatory cytokine ( IFN gamma , TNF alpha , IL 6 , and IL 12 ) production by spleen and lymph node cells in response to bovine type 2 collagen ( CII ) in vitro compared with wild type mice , paralleled in vivo by a significant reduction in serum anti CII IgG2a Ab level . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , infection results in increased intestinal expression of pro inflammatory cytokines such as IL 12 , IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pentoxifylline and thalidomide inhibited the in vitro stimulated production of TNF alpha , IL 1 beta and IFN gamma in blood mononuclear cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1 beta , TNF alpha and IFN gamma . ^^^ IL 8 concentration was measured by ELISA in supernatants of unstimulated and LPS , IL 1 beta , TNF alpha and IFN gamma stimulated cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , following stimulation with lipopolysaccharide or phorbol myristate acetate / calcium ionophore , peritoneal cells from H 2 ( b ) mice synthesised significantly more IL 1 beta , TNF alpha , TNFR and IFN gamma protein and IFN gamma mRNA than cells from congenic H 2 ( k ) or H 2 ( d ) mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We report on a cross sectional study on proliferation and cytokine production ( IFN gamma , IL 12 , IL 5 and TNF alpha ) by peripheral blood mononuclear cells ( PBMC ) , activated or not with phytohemagglutinin ( PHA ) in HIV 1 infected pregnant women , untreated or treated with zidovudine . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To study the role of tumour necrosis factor alpha ( TNF alpha ) on some of these metabolic responses to endotoxin , we have used transgenic mice expressing soluble tumour necrosis factor receptor 1 IgG fusion protein ( TNFR 1 IgG ) as well as TNF alpha knockout ( KO ) , lymphotoxin alpha ( LT alpha ) KO , and interferon gamma receptor ( IFN gamma R ) KO mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Levels of IL 12 , as well as IL 4 , IL 8 , IL 10 , IFN gamma , sCD 25 , TNF alpha and its soluble receptors were measured in the SF of various arthropathies , i . e . non inflammatory arthropathies : `` control ' ' meniscus pathology ( n = 21 ) , osteoarthritis ( n = 22 ) and chronic crystal arthritis ( n = 9 ) ; a non immune inflammatory arthropathy : acute crystal arthritis ( n = 11 ) ; 2 immune inflammatory arthropathies : reactive arthritis ( ReA ) ( n = 23 ) and rheumatoid arthritis ( RA ) ( n = 44 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , IP10 / mob 1 transcripts were strongly induced in peritubular cells by interferon alpha ( IFN alpha ) and IFN gamma , whereas , in isolated Sertoli cells , INF alpha and TNF alpha were the only potent inducers . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Role of IL 12 in macrophage activation during intracellular infection : IL 12 and mycobacteria synergistically release TNF alpha and nitric oxide from macrophages via IFN gamma induction . ^^^ Because IL 12 and mycobacteria also released IFN gamma from macrophages synergistically , and exogenous IFN gamma with mycobacteria enhanced TNF alpha and NO release synergistically , we examined the role of endogenous IFN gamma in IL 12 / mycobacteria stimulated macrophage activation . ^^^ Using macrophages from mice deficient in IFN gamma , we found that IL 12 / mycobacteria enhanced macrophage TNF alpha and NO release was mediated through endogenous IFN gamma . ^^^ We further demonstrated that IFN gamma and mycobacteria together had a selective effect on macrophage cytokine release because they released TNF alpha synergistically but not macrophage chemotactic protein 1 ( MCP 1 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Optimal monocyte mediated syncytiotrophoblast death requires both lipopolysaccharide ( LPS ) and IFN gamma and is inhibited by either anti tumor necrosis factor ( TNF ) antibody or epidermal growth factor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
LPS induces direct death of IFN gamma primed murine embryonic hepatocyte , BNL CL 2 cells in a TNF alpha independent manner . ^^^ Although it has been well known that the role of LPS on liver damage is mediated through TNF alpha , the mechanism by which LPS modulates the cytotoxicity of IFN gamma on hepatocytes has not yet been clearly demonstrated . ^^^ In addition , TNF alpha alone does not affect cell viability but rather it potentiates the cytotoxic effect of IFN gamma on BNL CL 2 cells . ^^^ However , the cell viability of IFN gamma / LPS treated cells is affected by the addition of polymyxin B but not by TNF binding protein 1 ( TNF BPI ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The activity of R . leguminosarum LPS was almost comparable to Salmonella endotoxin in terms of lethality , Limulus lysate gelating activity and in vivo tumor necrosis factor alpha ( TNF alpha ) , interleukin 1beta ( IL 1beta ) , interleukin 6 ( IL 6 ) and interferon gamma ( IFN gamma ) induction capacity . ^^^ Weak lethality of LPS from Mesorhizobium correlated with low capacity of this LPS to induce TNF alpha , IL 1beta , IL 6 and IFN gamma both in vivo and in vitro in murine splenocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lipopolysaccharide with IFN gamma also enhanced TNF alpha levels . ^^^ Combined use of TNF alpha and IFN gamma caused marked hepatocyte damage , while these cytokines alone did not . ^^^ CONCLUSIONS : We suggest that elevated endotoxin levels accompanying the development of liver injury may activate Kupffer cells to release TNF alpha leading to exacerbation of hepatocyte damage in cooperation with IFN gamma produced during liver injury . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Co stimulation of IFN gamma and TNF alpha significantly triggered the HC and NPC isolated at 0 h to produce NO . ^^^ As NO synthase inhibitors , Nomega Nitro L Arginine exacerbated the liver injury in mice and NG Monomethyl L Arginine enhanced the hepatotoxicity of IFN gamma and TNF alpha in vitro . ^^^ CONCLUSIONS : NO may be produced by HC and NPC under the co stimulation of IFN gamma and TNF alpha , and may play an important role for alleviating the liver injury . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The prevalence and levels of both cellular ( lymphocyte proliferation , tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and interleukin 10 ( IL 10 ) ) and humoral ( immunoglobulin G ) responses were determined . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our findings suggest that IFN gamma , TNF alpha , and IL 12 contribute to protection against MAC , whereas IL 10 is immunosuppressive . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
ALR islets were found to be remarkably resistant to two different combinations of beta cytotoxic cytokines ( IL 1beta , tumor necrosis factor alpha , and IFN gamma ) that destroyed islets from the related NOD and alloxan susceptible strains . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Profiles of cytokines ( interleukin ( IL ) 2 , IL 6 , IL 8 , IL 12 p 40 , TNF alpha , IFN gamma ) and granulocyte macrophage colony stimulating factor ( GM CSF ) were established in normal lactating cattle . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The differential effect of IFN beta and TGF b on IL 12 bioactivity was most pronounced upon IFN gamma synthesis , since IFN beta induced only marginal amounts of IL 10 and IL 12 and TGF beta diminished constitutive IL 10 production , while neither had a significant effect on TNF alpha production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Comparison of CVS infection in these two strains of mice indicated that decreased immune responsiveness is associated with : ( 1 ) an in vivo reduction of the percentages of Th 1 ( IL 2 , IFN gamma and TNF alpha ) but not of Th 2 ( IL 4 ) cytokine secreting T cells ; and ( 2 ) an in vivo increase of the percentages of CD 25 and CD 69 expressing splenocytes . ^^^ We propose that T cell activation through this receptor , in an environment of poor antigen presentation , results in a state of T cells characterized by the reduced production of IL 2 , TNF alpha and IFN gamma in vivo , the decreased responsiveness of splenocytes to ConA stimulation in vitro and the expression of the activation markers CD 25 and CD69 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment with TNF alpha and IFN gamma upregulated B 7 2 and induced de novo expression of B 7 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Some of them , i . e . ( R ) PMPA , ( S ) PMPA , and PMEG , stimulate secretion of TNF alpha and IL 10 in a concentration dependent manner , and enhance the IFN gamma induced secretion of TNF alpha . ^^^ Although less effectively , PMEG and ( R ) PMPA also increase production of TNF alpha and NO by the IFN gamma but not LPS co stimulated macrophages from C3H / HeJ mice , which are otherwise hypo responsive to major immune stimuli provided by IFN gamma and LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After 48 hr incubation of PBMC with rML , nML , and eML , induction of IL 1 beta , TNF alpha , IL 2 , IL 6 , and IL 10 but not IFN gamma was measured . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The mutation generates an amino acid change that does not abrogate protein expression on the cellular surface but that severely impairs responses after the binding of IFN gamma ( CD 64 and HLA class 2 induction and tumor necrosis factor alpha and interleukin 12 production ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Following activation , Th 1 CD4 T cells produce in abundance the inflammatory cytokines TNF alpha , IFN gamma and lymphotoxin alpha ( LT alpha , also know as TNF beta ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
No differences were found for the production of IL 2 , IL 4 , IL 12 , TNF alpha , and IFN gamma between C 3 ( + / + ) and C 3 ( / ) mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma production by both cell populations was inhibited by coincubation with neutralizing mAb to IL 12 or IL 18 , while a mAb to TNF had much less effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Depletion of IFN gamma did , however , reduce the concentration of TNF detected in lung homogenates of challenged mice and largely prevented the weight loss associated with CTL mediated viral clearance . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In LPS resistant mice , i . p . injection of S . dublin flagellin or medium conditioned by wild type S . dublin induces serum IFN gamma and TNF alpha , whereas medium conditioned by aflagellic mutants has no effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here we provide evidence that resistance of TNFR 1 ( / ) and TNFR 2 ( / ) mice against Con A hepatitis is not due to an impaired production of the central mediators TNF and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We demonstrated significant correlations of cytokine mRNA and protein expression for TNF alpha , IL 10 , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To elucidate mechanisms of salivary gland pathology , the effects of interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) on cell cycle progression and integrin expression were evaluated in HSG acinarlike cells . ^^^ From this study , it was evident that the combination of IFN gamma and TNF alpha resulted in a block in G ( 1 ) phase for acinar cells before accumulation of the alpha 3 integrin variant or significant degenerative cellular changes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , the induction of IL 8 by various cytokines ( IL 1 alpha , TNF alpha , IL 6 , TNF beta , GM CSF , IFN gamma ) was significantly reduced in U 1 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We describe here four cases of B LAHS that include the following features : ( 1 ) HPS was the initial presentation ; ( 2 ) bone marrow involvement with large cell lymphomas was noted in all cases , despite lack of remarkable lymphadenopathy ; ( 3 ) no active infection with Epstein Barr virus as the etiological agent was confirmed ; ( 4 ) except for the spleen in one case , primary site of lymphoma could not be determined ; and ( 5 ) serum IL 6 , soluble IL 2 receptor , and IFN gamma but not TNF alpha and IL 1 beta , were significantly elevated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The levels of TNF alpha and IFN gamma PHA MNC CM increased in a PHA dose dependent manner and were much higher in the athletic group when compared to the controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Th 2 cells provided only a cell associated cofactor , whereas Th 1 cells secreted a soluble cofactor for IFN gamma as well , i . e . , TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Secretion of IL 6 , TNF alpha , and IFN gamma were demonstrated in all atherosclerotic lesions and IL 6 appeared to be the dominant cytokine , both in the lesions themselves as well as in the intimal medial junction . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Despite the presence of TNF alpha and IFN gamma , both being capable of sensitizing microglia to FasL , apoptosis of microglia is not a hallmark of inflammatory diseases of the CNS . ^^^ In the present study , TGF beta is found to counteract the effect of TNF alpha and IFN gamma to sensitize microglia to FasL mediated apoptosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Functionally , ICOS co induces a variety of cytokines including IL 4 , IL 5 , IL 6 , IFN gamma , TNF alpha , GM CSF , but not IL 2 , and superinduces IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Morbidity ( weight loss and paralysis ) and mortality of infected mice were associated with viral propagation , cytokine ( IL 6 , IL 10 , TNF alpha and IFN gamma ) production , induction of apoptosis and infiltration of inflammatory cells . ^^^ These results strongly suggest that , the modulation of TNF alpha and upregulation of IFN gamma would be a powerful anti virus strategy in cases of viral encephalitis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumour cell derived IL 8 production was upregulated by the addition of the pro inflammatory type 1 cytokines TNF alpha , IL 1 beta and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum levels of TNF alpha , interleukin 18 ( IL 18 ) / IFN gamma inducing factor ( IGIF ) , and interferon gamma ( IFN gamma ) at 1 h after LPS administration were significantly lower in the ebselen treated group . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The treatments included : ( 1 ) TNF alpha and lipopolysaccharide ( LPS ) ; and ( 2 ) LPS , TNF alpha , interleukin 1beta ( IL 1beta ) and interferon gamma ( IFN gamma ) incubated for 8 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The addition of the beta agonist increased the inhibitory effect of tested PDE inhibitors on the production of TNF alpha and IL 1 beta Type 4 , type 3 and nonselective PDE inhibitors were effective in inhibiting the production of IFN gamma and IL 2 in a dose dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Effect of TNF alpha and IFN gamma on epithelial barrier function in rat rectum in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
HIV infection of human immune cells induced an increase in cytokine release for example , TNF alpha , IL 1 beta , IFN alpha , and IFN gamma downregulating the epithelial barrier function of the human colonic epithelial cell line HT 29 / B6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inflammation and infection lead to the upregulation of the transport and processing of food proteins ; for example , IFN gamma increases the rate of transcytosis and alters , like TNF alpha , the tight junction permeability . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
They all express mRNA of cytokines IL 1 alpha , IL 1 beta , IL 8 , and TNF alpha , but not of IFN gamma , IL 2 , and IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found that macrophage activating cytokines , including TNF alpha , IFN gamma , IL 1alpha , and GM CSF , when added prior to M . avium , increased the transport of iron into the phagosome . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
INTERVENTIONS : Confluent rat vascular smooth muscle cells ( VSMC ) were incubated for 24 hrs and 48 hrs with PCT ( 1 ng / mL , 10 ng / mL , 100 ng / mL , 1 , 000 ng / mL , 5 , 000 ng / mL ) alone or with the combination of tumor necrosis factor alpha ( TNF alpha , 500 U / mL ) plus interferon gamma ( IFN gamma , 100 U / mL ) . iNOS gene expression was measured by qualitative as well as quantitative polymerase chain reaction analysis , NO release was estimated by the modified Griess method . ^^^ However , PCT ameliorated TNF alpha / IFN gamma induced iNOS gene expression in a dose dependent manner ( maximal inhibition at PCT 100 ng / mL by 66 % for 24 hrs and 80 % for 48 hrs ) . ^^^ CONCLUSIONS : We conclude that recombinant PCT inhibits the iNOS inducing effects of the proinflammatory cytokines TNF alpha / IFN gamma in a dose dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
At different time points we determined the number of colony forming units of bacteria in the liver of infected animals and paralled these with the plasma levels of interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) and interleukin 6 ( IL 6 ) measured by enzyme immunoassays . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Supernatants were collected and analysed for the interleukins IL 1beta , IL 2 , IL 6 , and IL 10 , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunologic studies included lymphocyte subset analysis by flow cytometry , measurement of interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) production following in vitro stimulation of diluted whole blood ( DWB ) and peripheral blood mononuclear cells ( PBMC ) by phytohemagglutinin ( PHA ) , anti CD 3 as well as purified protein derivative of tuberculin ( PPD ) , and in four cases with different amounts of the very mycobacterium , which caused disease in these patients . ^^^ Following alpha CD 3 stimulation of PBMC , NTM patients showed lower IFN gamma ( P < 0 . 00005 ) and lower TNF alpha ( P < 0 . 02 ) . ^^^ The lower production capacity of IFN gamma and TNF alpha of NTM patients compared to the MTb patients points to an immunologic imbalance forming the basis for their increased susceptibility to pulmonary infections by nontuberculous mycobacteria . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF was not detectable in any of the cell lines , and TGF beta , alpha FGF and HG were not influenced by IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The results of this study indicated a role of in vivo macrophage stimulatory factors , notably cytokines such as TNF alpha and IFN gamma in host ' s serum , as well as parasite antigens , which may act singly or in concert , in the process of enhanced erythrocyte destruction , hence anaemia by the mononuclear phagocytic system ( MPS ) during bovine trypanosomosis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of surface membrane antigens on peripheral blood monocytes ( PBM ) of cattle of the Boran and N ' Dama breeds activated with recombinant cytokines ( TNF alpha and IFN gamma ) and during experimental infection with Trypanosoma congolense was investigated using monoclonal antibodies ( MoAbs ) and fluorescein activated cell sorter ( FACS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To observe the status of Th1 / Th2 balance in children with JCA , the cytokines IL 4 , IFN gamma , TNF alpha and IL 6 were measured in the tissue culture of the synovial cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumour necrosis factor ( TNF ) activity was assayed by measuring its cytotoxic activity on L 929 cells , interleukin 6 ( IL 6 ) and interferon gamma ( IFN gamma ) were assayed by enzyme linked immunosorbent assay ( ELISA ) , whereas NO levels were detected by Griess colorimetric reactions in the culture supernatant of macrophages incubated with IFN gamma , TNF or NO and subsequently exposed to either BCG 1 or BCG S . ^^^ The highest levels of TNF and IFN gamma were observed in macrophages treated with BCG S and BCG 1 , respectively . ^^^ We also found a different susceptibility of the bacilli to exogenous treatment with IFN gamma and TNF which were capable of killing 60 and 70 % of both bacilli , whereas NO was capable of killing about 98 and 47 % of BCG 1 and BCG S , respectively . ^^^ The amount of bacilli proportionally decreased with IFN gamma and TNF , suggesting a cytokine related cytotoxic effect . ^^^ In this particular case , the IFN gamma and TNF were involved in the activation of macrophage bactericidal activity . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The concentrations of IFN gamma , IL 2 , IL 6 , IL 12 , TNF alpha and its soluble receptors R 1 , R 2 were estimated in non stimulated and PHA ( Sigma , 5 microg / ml ) stimulated culture supernatants using ELISA Endogen kits according to the manufacturer ' s instructions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We also studied the effect of inflammatory cytokines including interferon gamma ( IFN gamma ) , tumor necrosis factor alfa ( TNF alpha ) , and IFN alpha on the expression of IGF 1 and IGFBPs in the fibroblasts . ^^^ The expression of IGFBP 5 mRNA in PF was downregulated by IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 12 , IFN gamma , and TNF alpha released from mononuclear cells inhibit the spread of varicella zoster virus at an early stage of varicella . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our data show that IL 4 may play a protective role in defense against mycobacteria , although IFN gamma and TNF alpha play major roles in it . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression levels of tumor necrosis actor alpha ( TNF alpha ) , gamma interferon ( IFN gamma ) , interleukin 1 beta ( IL 1beta ) , intercellular adhesion molecule 1 ( ICAM 1 ) and inducible nitric oxide synethetase ( iNOS ) were highest in the cerebellum of infected animals , correlating well with pathologic observations of sequestration of parasitized erythrocytes in this region of the brain . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The correlation of cytokines TNF alpha , IFN gamma , Epo with anemia in rheumatoid arthritis ] . ^^^ OBJECTIVE : To elucidate the characteristics of anemia of chronic disease ( ACD ) in rheumatoid arthritis ( RA ) , and the role of TNF alpha , IFN gamma and Epo in the pathogenesis of ACD . ^^^ METHOD : Serum TNF alpha and IFN gamma levels in RA patients and serum Epo levels in RA with ACD were measured by ELISA . ^^^ The effects of TNF alpha and IFN gamma on cobalt induced erythropoietin production in human hepatic cancer cells ( HepG 2 ) were examined . ^^^ The effect of TNF alpha and IFN gamma erythroid colony formation ( CFU E and BFU E ) of normal bone marrow cells , and the effect of serum from RA patients and Epo on CFU E yields of RA bone marrow cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma also synergized in cytostatic / cytotoxic effects with TNF alpha against B 78 melanoma in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Consistent with this , potent inducers of ICAM 1 such as IFN gamma , TGF beta , and TNF alpha were absent in the culture supernatant , but a significant amount of IFN type 1 was present . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Intracellular cytokines such as TNF alpha , IL 1 beta , IL 2 and IFN gamma were measured in peripheral mononuclear cells of 15 patients in acute stage , in 5th and 30th days after gammaglobulin treatment , utilizing specific staining and analysis by flow cytometry showing no sole characteristic profile . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In monocytes , macrophages and dendritic cells alpha MSH inhibits the production and activity of immunoregulatory and proinflammatory cytokines such as IL 2 , IFN gamma , TNF alpha and IL 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Overproduction of IFN gamma and TNF alpha from natural killer ( NK ) cells is associated with abnormal NK reactivity and cognitive derangement in Alzheimer ' s disease . ^^^ NK cell cytotoxicity ( NKCC ) and the generation and release of IFN gamma and TNF alpha ( spontaneous and modulated by IL 2 ) from pure NK cells ( CD 16+ , CD 56+ , CD 3 ) were studied together with circulating IFN gamma and TNF alpha levels and cognitive function in 22 old patients with DAT and 15 healthy old subjects . ^^^ Increased spontaneous and IL 2 induced release of IFN gamma and TNF alpha from NK cells were found in DAT patients compared to healthy subjects ( p < 0 . 001 ) , whereas no difference of serum IFN gamma and TNF alpha was demonstrated between DAT and control groups . ^^^ Significant negative correlations among the spontaneous release of IFN gamma and TNF alpha from NK and the decrease of the score of cognitive function ( MMSE ) were found in patients with DAT . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The study was carried out together with the evaluation of natural killer ( NK ) cell function : cytotoxic activity ( NKCC ) and TNF alpha and IFN gamma release after exposure to IL 2 ( 100 U / mL ) . ^^^ The activation of hypothalamo pituitary adrenal ( HPA ) axis and the decrease of GH , and MLT nocturnal peaks were associated with normal NKCC and TNF alpha / IFN gamma in healthy elderly subjects , whereas in DAT patients the same neuroendocrine changes occurred together with abnormal NKCC ( spontaneous and IL 2 / IFN beta modulated ) and with alterations of TNF alpha / INF gamma generation from NK . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To elucidate the metabolic , endocrine , and immunologic consequences of the observed disturbance , we measured serum lipids , glucose , C peptide , ACTH , plasma , urinary cortisol , and cytokines IL 2 , IFN gamma , Il 4 , IL 10 , Il 12 , and TNF alpha in 36 patients with FR and in a control group without FR . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Some of the specimens expressed MIP 1alpha , RANTES , GM CSF , M CSF , TNF alpha , PDGF B and bFGF , but no expression of IL 2 , IL 4 , IFN gamma , IGF 1 , EGF and KGF was detected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We determined the serum levels of cytokines ( IL 2 , IFN gamma , IL 4 , IL 10 , IL 5 , IL 6 , IL 1 beta , TNF alpha , IL 8 ) , soluble receptors ( sIL2R , sIL6R ) , Bcl 2 protein and p 53 antibody in a group of healthy subjects . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The activity of phagocytes was also investigated in the cultures with cytokines : IFN gamma , TNF alpha , GM CSF , IL 4 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Marked but transient elevations in tumor necrosis factor alpha , IFN gamma , and IL 6 in the cerebrospinal fluid were observed within 4 h of CTL infusion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : NF kB activity in three permanent cell lines ( Hep 2 , HepG 2 , HT 29 ) and in primary RCC cell lines was measured after incubation with tumor necrosis factor alpha ( TNFalpha ) , IFNalpha , IFN gamma , TNFalpha+IFNalpha , and IFNgamma+TNFalpha , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 10 augments the IFN gamma and TNF alpha induced TARC production in HaCaT cells : a possible mechanism in the inflammatory reaction of atopic dermatitis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In particular , the lymphocyte subset distribution , the cytotoxic activity and the intracellular production of tumour necrosis factor ( TNF ) alpha and IFN gamma by CD 4 ( + ) , CD 8 ( + ) and CD 56 ( + ) cells were investigated . ^^^ TNF alpha and IFN gamma production by CD 4 ( + ) , CD 8 ( + ) and CD 56 ( + ) lymphocytes was significantly enhanced compared with that of patients at diagnosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When PLC / PRF / 5 cells stimulated by various cytokines ( interleukin 1 alpha ( IL 1 alpha ) , IL 1 beta , tumor necrosis factor alpha ( TNF alpha ) , TNF beta , IL 6 , tumor growth factor alpha ( TGF alpha ) , TGF beta , and interferon gamma ( IFN gamma ) were compared , the most effective was IL 1 , followed by TNF alpha and IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After activation with TNF alpha or IFN gamma or without prior treatment , the macrophages were infected with M . tuberculosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A comparative study was done using J774A . 1 and J774A . 1 derived transfected cells ( J774A . 1 C . 1 ) containing antisense tumor necrosis factor alpha ( TNF alpha ) plasmid to determine the role of endogenous TNF alpha on nitric oxide production as well as on the growth of Mycobacterium microti in interferon gamma ( IFN gamma ) and lipopolysaccharide ( LPS ) treated cells . ^^^ On stimulation with IFN gamma and LPS a higher level of NO was observed in J774A . 1 cells compared to J774A . 1 C . 1 which indicated that endogenous TNF alpha is required for the production of NO . ^^^ J774A . 1 C . 1 cells infected with M . microti produced a significant amount of NO when exogenous TNF alpha was added along with IFN gamma and LPS and the concentration of intracellular bacteria decreased almost to that in IFN gamma and LPS treated parental J774A . 1 cells . ^^^ Addition of exogenous TNF alpha even in the presence of L NMA in J774A . 1 C . 1 cells could also partially restore intracellular growth inhibition of M . microti caused by IFN gamma and LPS . ^^^ TNF alpha is probably required for the production of NO in J774A . 1 cells by IFN gamma and LPS but TNF alpha and NO are independently involved in the killing of intracellular M . microti with IFN gamma and LPS . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate the potential role of tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) in inducing apoptosis of human trophoblast during early pregnancy and its mechanism . ^^^ Having cultured with TNF alpha and IFN gamma for 36 hours , their cytotoxic activity was determined by viable cell calculation . ^^^ While combined with IFN gamma , TNF alpha decreased the number of viable cells more markedly . ^^^ CONCLUSION : TNF alpha and IFN gamma could induce apoptosis of human trophoblasts during early pregnancy , partly through the mechanism of ROS . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PURPOSE : To investigate the mRNA expression of the receptors for tumour necrosis factor alpha ( TNFRp 55 , TNFRp 75 ) , interferon gamma ( IFN gamma R alpha , IFN gamma R beta ) , interleukin 10 ( IL 10R , CRFB 4 ) and transforming growth factor beta ( TGF beta RII ) on human retinal pigment epithelium ( RPE ) cells and to modulate this expression with the pro inflammatory cytokines TNF alpha and IFN gamma as stimulators . ^^^ METHODS : The cells were cultured in the presence of TNF alpha ( 10 ng / ml ) , IFN gamma ( 1000 U / ml ) or a combination of both for 24 h , 48 h and 72 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The proinflammatory cytokines TNFalpha , IL 1beta , IL 6 , IL 8 , and IFN gamma are associated with inflammation in synovial joints and connective tissue destruction . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PBMC co incubation with either S . typhi strain infected U 937 cells or autologous monocytes resulted in significant increases in IL 12 , TNF alpha , and IFN gamma secretion . ^^^ In similar experiments using HPNKC samples instead , infected U 937 cells significantly increased IL 12 and IFN gamma , but not TNF alpha secretion . ^^^ PBMC co incubation with non infected U 937 cells , but not with non infected monocytes , significantly increased supernatant IL 12 and TNF alpha levels ( no significant changes in IFN gamma were recorded ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In particular among tested factors , we observed a gradient of chemotactic activity as follows : HGF > > RANTES > PDGF A > PDGF B > FGF > > TNF alpha > IFN gamma > EGF . ^^^ Endothelial and myoblast expression of Pax 3 ( a transcription factor expressed by embryonic migrating myogenic cells ) and cytokine transcripts ( TNF alpha , IFN gamma ) was also monitored either at the basal level and after transmigration . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Further examination of the cytokines essential for the generation of HCMV permissive Allo MDM identified gamma interferon ( IFN gamma ) but not interleukin 1 or 2 , tumor necrosis factor alpha , or granulocyte macrophage colony stimulating factor as critical components in the generation of these macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To determinate the potential role of interleukins ( IL 1 , IL 2 , IL 4 , IL 6 , IL 10 ) , interferon ( IFN gamma ) , tumor necrosis factor ( TNF alpha ) , and transforming growth factor ( TGF beta 2 ) in anorexia nervosa , serum concentrations of these cytokines were measured in patients suffering from anorexia nervosa in comparison to healthy subjects . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In A 549 human lung epithelial cells , a combination of cytokines tumor necrosis factor alpha , interleukin 1 beta , and IFN gamma ( TNF alpha , IL 1 beta , and IFN gamma ) function synergistically for induction of hiNOS transcription . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Third , the expression of both proinflammatory cytokines ( TNF alpha , IFN gamma ) and anti inflammatory cytokines ( IL 10 , TGF beta ) in mRNA levels were increased at 4 weeks after infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Delayed clearance of AdCMV lacZ from the livers of FasL defective B6 . gld mice , but not perforin deficient B6 . pfp ( / ) mice , was noted despite no significant differences in initial hepatic CD 8 ( + ) T cell IFN gamma or TNF responses or in activation of intrahepatic cytotoxic lymphocytes cells capable of killing AdCMV lacZ infected fibroblast targets . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The transactivator protein of human T lymphotropic virus 1 ( HTLV 1 ) , Tax , has been associated with the up regulation of several host cell genes , including interleukin 2 ( IL 2 ) , the IL 2 receptor alpha ( IL 2Ralpha ) chain ( CD 25 ) , interferon gamma ( IFN gamma ) , and tumor necrosis factor ( TNF ) . ^^^ Surprisingly , the results show that intracellular Tax expression is associated with rapid up regulation of IFN gamma but not TNF or IL 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , the addition of LPS , LTA , LPS plus IFN gamma , or tumor necrosis factor alpha ( TNF alpha ) plus prostaglandin E 2 to such cells again resulted in the rapid induction of apoptosis in the majority of cells , together with a reduced production of IL 12 p 70 and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We review possible genetic host susceptibility factors for BU that are relevant in other mycobacterial diseases : natural resistance associated macrophage protein 1 ( NRAMP 1 ) , HLA DR , vitamin D 3 receptor , mannose binding protein , interferon gamma ( IFN gamma ) receptor , tumour necrosis factor alpha ( TNF alpha ) , interleukin ( IL ) 1 alpha , 1 beta and their receptor antagonists ; and IL 12 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Semi quantitative reverse transcription polymerase chain reaction method confirmed the upregulated expression levels in DCs for TNFRII , TNF alpha , alpha catenin and downregulation of IFN gamma , GM CSFR on four different donor samples of DCs and monocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This mini array is a multiplexed , sandwich type ELISA that measures the concentration of seven different human cytokines TNF alpha , IFN alpha , IFN gamma , IL 1 alpha , IL 1 beta , IL 6 , and IL 10 from a single sample in each well of a 96 well plate . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Enhanced proliferation and increased IFN gamma production in T cells by signal transduced through TNF related apoptosis inducing ligand . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Melatonin suppressed the release of TNF alpha by LPS or IFN gamma activated macrophages but failed to inhibit nitric oxide ( NO ) release . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulated peripheral blood derived CD4+ and CD8+ T MS patients express significantly more CD4+ and CD8+ T cells were analyzed for IFN gamma , IL 2 , TNF alpha , IL 4 , IL 10 and IL 13 production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : No differences between FMS and controls were found by measuring IL 1beta , IL 2 , IL 10 , serum IL 2 receptor ( sIL 2R ) , interferon gamma ( IFN gamma ) , and tumour necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We show that activation of T cells with anti CD 3 alone elicits a small IFN gamma and TNF alpha response with no tissue injury . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The present study used IFN gamma or TNF alpha gene disrupted mice and IFN gamma antibody treated mice to demonstrate that lipopolysaccharide ( LPS ) induced systemic IDO is largely dependent on TNF alpha rather than IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A decreased expression of TNF alpha , IL 1 beta , IL 6 , monocyte chemoattractant protein ( MCP ) 1 , and macrophage inflammatory protein ( MIP ) 2 mRNAs was observed in the iris ciliary body and the retina from IL 13 treated rats , whereas IFN gamma was upregulated in the iris ciliary body . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After stimulation by arecoline , the amounts of IL 2 , TNF alpha , TGF beta and IFN gamma secreted by mononuclear cells were measured using the ELISA method . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The data obtained showed an increase of 65 and 52 % in mRNA expression for IL 4 and TNF respectively and a decrease of 59 and 55 % in mRNAs for IFN gamma and IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we show that bromelain , a mixture of cysteine proteases , can enhance IFN gamma mediated nitric oxide and TNFalpha production by macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma and TNF alpha were produced by ten out of ten PHAstimulated T cell lines from CCC patients ; IL 2 and IL 10 were produced by four out of ten and one out of ten lines , respectively ; IL 4 , IL 1alpha , IL 1beta , IL 6 and IL 12 were undetectable . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor ( TNF ) is also a major player in the resolution of Listeria infections and is suggested to have more global effects than can be explained by the induction of IFN gamma alone . ^^^ Tumor necrosis factor ( TNF ) is also a major player in the resolution of Listeria infections and is suggested to have more global effects than can be explained by the induction of IFN gamma alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine ( IFN gamma , TNF alpha ) responses , measured by intracytoplasmic staining after peptide stimulation , also were detectable in CD45RO+ and RA+ subsets as well as CD28+ and CD 28 subsets . ^^^ Of other markers that were heterogeneous in both lytic and latent epitope populations , CCR 7 gave the best discrimination of functionality ; thus , CCR7+ cells consistently failed to give an IFN gamma or TNF alpha response , whereas many CCR 7 cells were responsive . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The efficacy of the Vbeta6+ clone was associated with elevated production of IFN gamma , TNF alpha , and GM CSF compared with that of the Vbeta14+ clone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Analysis using a quantitative RT PCR included IFN gamma , IL 1beta , IL 2 , IL 4 , IL 5 , IL 6 , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It is demonstrated that similar to interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) induces coordinated changes at different steps of the major histocompatibility complex ( MHC ) class 1 processing and presentation pathway in nonprofessional antigen presenting cells ( APCs ) . ^^^ Significantly , it was demonstrated that the effects of TNF alpha observed in this experimental system were not mediated through the induction of IFN gamma . ^^^ These results suggest that TNF alpha may be useful when a concerted up regulation of the MHC class 1 presentation machinery is required but can not be achieved by IFN gamma . ( Blood . 2001 ; 98 : 1108 1115 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The principal conclusion is that non LPS parts of N . meningitidis may play a role in the pathogenesis of meningococcal sepsis by inducing substantial TNF alpha , IL 1beta , and IFN gamma production . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lymphotoxin alpha ( LT alpha ) represents a Th 1 cytokine with proinflammatory activities in LP , as has recently been demonstrated for interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^ In cell stimulation experiments , it could be shown that IFN gamma induces LT alpha and TNF alpha mRNA in the human squamous cell line HaCaT , concomitant with upregulation of MHC class 2 and intercellular adhesion molecule 1 , which could also be observed on lesional keratinocytes in LP . ^^^ Our results suggest that stimulation of keratinocytes with IFN gamma results in the upregulation of proinflammatory cytokines such as LT alpha and TNF alpha as well as MHC class 2 , which map to the same gene region of immunoregulatory genes on chromosome 6 and may be involved in the induction and maintenance of the disease . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
AMB caused a dose dependent increase of NO generation in interferon gamma ( IFN gamma ) stimulated rat and mouse astrocytes , as well as in IFN gamma + tumor necrosis factor alpha ( TNF alpha ) activated rat astrocytoma cell line C 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
FANCC interacts with Hsp 70 to protect hematopoietic cells from IFN gamma / TNF alpha mediated cytotoxicity . ^^^ The Fanconi anemia ( FA ) complementation group C gene product ( FANCC ) functions to protect hematopoietic cells from cytotoxicity induced by interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) and double stranded RNA ( dsRNA ) . ^^^ In whole cells , Hsp 70 FANCC binding and protection from IFN gamma / TNF alpha induced cytotoxicity were blocked by alanine mutations located in a conserved motif within the Hsp 70 interacting domain of FANCC . ^^^ We therefore conclude that FANCC acts in concert with Hsp 70 to prevent apoptosis in hematopoietic cells exposed to IFN gamma and TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , high levels of interleukin 6 ( IL 6 ) , tumor necrosis factor alpha ( TNF alpha ) , IL 12 , IL 10 , gamma interferon ( IFN gamma ) , and IL 4 production were detected in lung cells , and nitric oxide ( NO ) production was high in culture supernatants of bronchoalveolar lavage cells . ^^^ High levels of IFN gamma , IL 6 , TNF alpha , IL 12 , IL 10 , and NO were detected in the culture supernatants . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Sequential determination in serum of interleukin 1 beta ( IL 1 beta ) , necrosis tumoral factor alpha ( TNF alpha ) , gamma interferon ( IFN gamma ) , interleukin 6 ( IL 6 ) , and erythropoietin ( EPO ) in 25 patients with chronic bacterial infectious diseases and ACD criteria . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In particular , when CpG containing PS ODN are administered to mice , activation of macrophages and dendritic , NK , T , and B cells occurs , resulting in the release of an array of cytokines , including interleukin 12 ( IL 12 ) , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) . ^^^ In this report we show that when CpG containing SALP are administered intravenously to ICR mice the plasma concentrations of IL 12 , IFN gamma , IL 6 , monocyte chemoattractant protein 1 , and TNF alpha are greatly increased compared with the same dose of free ODN . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PROBLEM : How do major histocompatibility complex ( MHC ) 1 positive / negative choriocarcinoma ( CC ) cells affect the production of tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , interleukin 10 ( IL 10 ) , interleukin 4 ( IL 4 ) , and granulocyte macrophage colony stimulating factor ( GM CSF ) by decidual CD56++ cells ( large granular lymphocytes [ LGL ] ) , LGL depleted decidual cells ( WASH ) and unseparated decidual cells ( DEC ) in cocultures . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Rat tumor necrosis factor alpha ( rTNF alpha ) , interferon gamma ( IFN gamma ) , IL 10 , and hIL 10 were measured in graft tissue and recipient plasma by ELISA and detected by immunohistochemistry ( IHC ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma treatment increased macrophage secretion of TNF alpha but still reduced their MMP activity . ^^^ In conclusion , we show that mycobacterial infection induces MMP 9 activity both in vitro and in vivo and that this is regulated by TNF alpha , IL 18 , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : DA rats were immunized by injecting bovine myelin basic protein ( MBP ) . mRNA of cytokines , such as IL 6 , IL 10 , TNF alpha , TGF beta 1 , IFN gamma , and iNOS , were detected by RT PCR . ^^^ RT PCR studies demonstrated a strong expression of IFN gamma , IL 6 , IL 10 , TGF beta 1 , TNF alpha , and iNOS mRNA in spinal cord 13 d after immunization . ^^^ However IFN gamma , IL 10 , TNF alpha , and iNOS mRNA expression ( on d 13 ) was suppressed by Am 80 , except in the case of IL 6 , hence the effect of Am 80 on the expression of IL 6 mRNA was examined in additional experiments . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inducible iNOS was detected in cultured PBMC by immunofluorescence , immunoblot and flow cytometry with or without IL 1 beta + TNF alpha , IL 4 or IFN gamma from day 1 to day 5 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines are commonly classified in one or the other category : interleukin 1 ( IL 1 ) , tumor necrosis factor ( TNF ) , gamma interferon ( IFN gamma ) , IL 12 , IL 18 and granulocyte macrophage colony stimulating factor are well characterized as pro inflammatory cytokines whereas IL 4 , IL 10 , IL 13 , IFN alpha and transforming growth factor beta are recognized as anti inflammatory cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These data demonstrate the coordinated up regulation of two types of mucin genes in response to T . spiralis infection and may form the basis of an innate mucosal response independent of IFN gamma , TNF , and IL 4 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human fetal lung fibroblasts ( HFL ) were cultured in type 1 collagen gels and floated in medium containing TNF alpha , IL 1 beta , or IFN gamma alone or in combination ( cytomix ) . ^^^ The potency order was IL 1 beta , TNF alpha , IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 10 production was more profoundly influenced by low doses of H2O2 than IFN gamma , TNF alpha , and IL 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In MCMV persistent infection , when MCMV was detected only in the salivary gland , T cells of mice were modified to produce a massive amount of such cytokines as TNF alpha and IFN gamma upon in vivo stimulation with anti CD 3 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In whole blood from 5 additional NHV , selected targets ( intracellular interleukin 2 [ IL 2 ] , tumor necrosis factor alpha [ TNF alpha ] , and interferon gamma [ IFN gamma ] ) were measured in phorbol myristate acetate ( PMA ) ionomycin stimulated T lymphocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
COX 2 inhibitors also inhibited the differentiation of these cells induced by interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) and retinoic acid ( RA ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The immunological mechanisms involved in this antitumor effect were suggested by a decisively increased secretion of tumor necrosis factor alpha TNFTnTNa and IFN gamma from CD 4 ( + ) and CD 8 ( + ) T cells and a markedly up regulated expression on CD 8 ( + ) T cells of high affinity IL 2 receptor alpha chain ( CD 25 ) , costimulatory molecule CD 28 , and adhesion molecule lymphocyte function associated antigen 2 ( LFA 2 / CD2 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although MFP 14 reduced anti CD 3 and / or LPS induced blood levels of IFN gamma , TNF alpha and IL 12 it increased IL 4 and IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : We examined the effects of IFN gamma , tumor necrosis factor alpha ( TNF alpha ) , and granulocyte macrophage colony stimulating factor ( GM CSF ) on CD 40 expression on CD68+ synovial macrophage lineage cells ( SMC ) . ^^^ IFN gamma , but not TNF alpha or GM CSF , markedly upregulated CD 40 expression on CD68+ SMC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines produced by type 1 T helper cells , such as TNF alpha and IFN gamma , markedly potentiate apoptosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The quantitative determination of IL 1alpha , IL 2 , IL 4 , IL 6 , IL 10 , TNF alpha and IFN gamma was performed in mouse sera by ELISA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 18 in the same concentration range stimulated the production of IL 12 , TNF alpha , and IFN gamma in culture of PBMC ; however , IL 18 induced expression of ICAM 1 in monocytes was not inhibited by anti IL 12 , anti TNF alpha , or anti IFN gamma Ab , suggesting the independence of the upregulating effect of IL 18 on endogenous IL 12 , TNF alpha , and IFN gamma production . ^^^ On the other hand , anti ICAM 1 and anti LFA 1 Abs inhibited IL 18 induced production of three cytokines , IL 12 , IFN gamma , and TNF alpha , by 60 and 40 % , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These same mutations failed to complement the characteristic hypersensitive apoptotic responses of FA C cells to tumor necrosis factor alpha ( TNF alpha ) and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , the effect of interferon gamma ( IFN gamma ) on tumour necrosis factor alpha ( TNF alpha ) producing capacity of whole blood after severe trauma and cardiac surgery was investigated . ^^^ Pre incubation with IFN gamma in vitro increased endotoxin induced TNF alpha production in volunteers ' and all patients ' blood specimens in a dose dependent manner . ^^^ IFN gamma prompted an elevation of cytokine synthesis in CPB patients ' blood which equalled that of volunteers , whereas it caused a lower rise in TNF alpha production in blood of multiply injured patients , reaching levels of untreated donors only after incubation with 100 U / ml IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using IFN gamma R ( / ) and the respective wild type mice , we demonstrate that although sensitization to LPS and killed Listeria monocytogenes is exclusively IFN gamma dependent , an IFN gamma independent , moderate sensitization to certain TNF alpha inducing constituents in bacteria may develop in parallel . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of cultured RASMC with LPS and IFN gamma resulted in an increase of nitrite , tumour necrosis factor ( TNF alpha ) production and induction of iNOS mRNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , a bacterial mutant that does not induce IL 1 alpha expression but induces normal levels of IL 1 beta , TNF alpha , and IFN gamma , causes greatly reduced intestinal inflammation and is attenuated by LD ( 50 ) analysis in the C57BL / 6 mouse model . ^^^ These results demonstrate a distinct and unrecognized role for IL 1 alpha in inducing intestinal inflammation that can not be compensated for by the endogenous levels of IL 1 beta , TNF alpha , or IFN gamma that are produced in response to Y . enterocolitica . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 12 ' s chemotactic mechanism seemed mediated by overproduction and release of IL 8 by human PMNs in the presence of LPS , an effect involving TNF alpha and IFN gamma secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , the median tumor necrosis factor ( TNF ) alpha response was lower in case patients than control patients after stimulation with lipopolysaccharide ( 833 vs . 1149 pg / mL ; P= . 06 ) and lipopolysaccharide plus IFN gamma ( 3301 vs . 4411 pg / mL ; P= . 04 ) . ^^^ These abnormalities in resting IL 8 and lipopolysaccharide induced TNF alpha production were not associated with IFN gamma or IL 12 abnormalities and were detected up to several years after cure of disease , suggesting an abnormality in innate immunity . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have investigated the proinflammatory cytokines IL 6 , IL 1beta , TNF alpha and IFN gamma for their potential role in the regulation of FI expression . ^^^ IL 6 , but not IL 1beta , TNF alpha or IFN gamma also increased FI protein levels in cell culture supernatants by about five fold as determined by a semiquantitative immunoblot using a novel monoclonal antibody specific for rat FI . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Three other proinflammatory cytokines , IL 1beta , IFN gamma and TNF alpha , were alone or in combination , without any effect on the regulation of FI . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OBJECTIVE : To determine the relations between macrophage migration inhibitory factor ( MIF ) , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and cortisol in patients with systemic inflammatory response syndrome ( SIRS ) and to determine whether their levels correlate with patient survival . ^^^ MEASUREMENTS AND RESULTS : Serum MIF , TNF alpha , IFN gamma , and cortisol levels were measured serially when the patients were first identified as having SIRS ( day 0 ) , and on days 1 4 . ^^^ Peak MIF levels significantly correlated with peak TNF alpha levels ( r 2 = 0 . 448 , P = 0 . 002 ) , but did not correlate with peak levels of cortisol and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
ICE / mice , lacking mature IL 1beta and IL 18 , but also defective in IFN gamma and TNF production , were completely protected against both E . coli and S . typhimurium LPS . ^^^ In conclusion , TNF and LT play a central role in the lethality due to E . coli LPS , whereas the lethal effects of S . typhimurium LPS are mediated through mechanisms also involving other cytokines such as IFN gamma , IL 1 and IL 18 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lipid peroxides , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) in peritoneal fluid from infertile women with minimal and mild endometriosis ] . ^^^ OBJECTIVES : To assess the concentrations of tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) and lipid peroxides ( the marker of free radicals activity ) in peritoneal fluid ( PF ) of infertile women with minimal and mild endometriosis . ^^^ Lipid peroxides ( malonyldialdehyde and 4 hydroxynonenal ) , TNF alpha and IFN gamma concentrations were measured in the PF using commercially available kits . ^^^ In the group with endometriosis we have found a positive correlation ( R = 0 . 77 , p = 0 . 04 ) between the concentrations of TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we demonstrate that IL 3 induces a dose and time dependent down regulation of CCR 3 from the surface of human eosinophils comparable to the CCR 3 specific ligand eotaxin , whereas IL 5 , GM CSF , IL 4 , IL 10 , IL 13 , IFN gamma , and TNF alpha had no effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
T cells from IL 10 ( / ) mice expressed similar levels of IFN gamma , IL 2 , and IL 4 after mitogen stimulation ; however , macrophages showed a reduced TNF alpha production compared with IL 10 ( + / ) littermates . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha , IL 6 , IFN gamma , and IL 10 gene expression polymorphisms and the IL 4 receptor alpha chain variant Q576R : effects on renal allograft outcome . ^^^ We sought to confirm and add to some of the earlier findings by studying the impact of tumor necrosis factor alpha ( TNF alpha ) , interleukin 10 ( IL 10 ) , interferon gamma ( IFN gamma ) , and interleukin 6 ( IL 6 ) polymorphisms and the interleukin 4 ( IL 4 ) receptor alpha chain variant on posttransplant renal allograft outcome . ^^^ METHOD : TNF alpha , IL 6 , IFN gamma , and IL 10 gene promoter region polymorphisms were assayed genotypically by PCR SSP on 120 patients transplanted at the Albany Medical Center . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Conversely , ovariectomy , which abrogated basal 17 beta estradiol levels and increased the severity of EAE , enhanced the expression of MIP 1 alpha and MIP 2 that were over expressed by inflammatory mononuclear cells in SC . 17 beta estradiol inhibited expression of LT beta , TNF alpha , and IFN gamma in SC , but had no effect on IL 4 or IL 10 , indicating reduced inflammation but no deviation toward a Th 2 response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The plasma levels of a panel of cytokines and cytokine associated molecules ( IL 1alpha , IL 2 , IL 4 , IL 6 , IL 10 , IL 12 , IL 15 , macrophage colony stimulating factor ( M CSF ) , interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , soluble IL 2 receptor ( sIL 2R ) , soluble tumor necrosis factor receptor 1 or 2 ( sTNFRI or 2 ) ) were assessed in 56 plasma samples of 13 pediatric patients undergoing hematopoietic stem cell transplantation ( HSCT , bone marrow in 12 and cord blood in one ) from unrelated donors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
High levels of interleukin 12 ( IL 12 ) and gamma interferon ( IFN gamma ) were produced by lymphocytes from the draining lymph nodes of recovering animals , whereas IL 6 , tumor necrosis factor alpha , and IFN gamma were detected in the oral mucosae of both nave and infected mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both cytokines stimulated cytotoxicity by NK and CD 56 ( + ) T cells against K 562 targets , but not the production of IFN gamma , TNF alpha , IL 2 , or IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Delayed immune responses were observed in mutant mice , although comparable IFN gamma and TNF alpha blood levels as in wild type mice were detected 2 wk postinfection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This protective effect of ISS ODN was largely independent of tumor necrosis factor alpha ( TNF alpha ) , interleukin 12 ( IL 12 ) , nitric oxide , NADPH oxidase , alpha / beta interferon ( IFN alpha / beta ) , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
C . neoformans 52D induced tumor necrosis factor alpha ( TNF alpha ) , gamma interferon ( IFN gamma ) , and MCP 1 in the lungs of infected wild type ( WT ) and MIP 1alpha knockout ( KO ) mice by day 7 postinfection . ^^^ Alternatively , C . neoformans 145A infected WT and MIP 1alpha KO mice had diminished TNF alpha , IFN gamma , and macrophage chemoattractant protein 1 ( MCP 1 ) responses , indicating that virulent C . neoformans 145A evaded early innate host defenses . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The capacity of HBMEC to restrict Toxoplasma growth after IFN gamma stimulation was enhanced in the presence of tumor necrosis factor alpha ( TNF alpha ) . ^^^ In addition , we found that IFN gamma induced a strong induction of indoleamine 2 , 3 dioxygenase ( IDO ) activity in HBMEC , and this enzyme activity was enhanced by costimulation with TNF alpha . ^^^ The addition of excess amounts of tryptophan to the HBMEC cultures resulted in a complete abrogation of the IFN gamma TNF alpha mediated toxoplasmostasis . ^^^ We therefore conclude that IDO induction contributed to the antiparasitic effector mechanism inducible in HBMEC by IFN gamma and TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Trophoblast invasion may be inhibited by local cytokines , such as TGF betas but Thl type cytokines associated with pre eclapmsia and spontaneous abortions ( e . g . , IL 1 , TNF alpha , IFN gamma ) are not known to inhibit migration at in situ concentrations . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Synovial cells obtained from 6 patients with osteoarthritic joint disease were incubated for 24 hours with ( 1 ) or without ( 2 ) IL 1beta ( 1 ng / ml ) , TNF alpha ( 500 pg / ml ) , IFN gamma ( 10 ( 4 ) U / ml ) plus minocycline or doxycycline ( 10 ( 4 ) to 10 ( 6 ) M ) , diclofenac ( 10 ( 5 ) M ) , or cortisol ( 10 ( 5 ) M ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neutralization of interleukin 18 reduces severity in murine colitis and intestinal IFN gamma and TNF alpha production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MM treated brainstems also had lower level of mRNA for Thl ( IL 2 , IL 12Rbeta2 , IFN gamma ) , Th 2 ( IL 4 , IL 10 ) cytokines and TNF alpha and TGF beta compared to that in CsA and controls groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effect of Ifx was investigated in these in situ activated T cell cultures regarding the IFN gamma production , proliferation , transmembrane TNF alpha expression , cytolysis and apoptosis . ^^^ T cell cultures from patients with Crohn ' s disease produced significantly higher levels of IFN gamma ( < 0 . 001 ) and TNF alpha ( P=0 . 04 ) than T cell cultures from healthy controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The treatment of mutant cells with IFN gamma did not restore the defect of NO or TNF alpha production on LPS treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
T helper ( Th ) 1 cells secreting cytokines such as IFN gamma and TNF alpha are crucial for an effective elimination of these bacteria . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although the blood levels of soluble TNF and IFN gamma were increased in transgenic animals after challenge with Con A , no damage of hepatocytes could be detected , as assessed by the lack of increase in plasma transaminase activities and the absence of TUNEL staining in the liver . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
More importantly , the suppressive effect of adenosine and CGS 21680 on IL 12 production was significantly enhanced in cells pretreated with either IL 1 ( 10 U / ml ) or TNF alpha ( 100 U / ml ) but markedly attenuated in cells pretreated with IFN gamma ( 100 U / ml ) . ^^^ Similarly , IL 1 and TNF alpha treatment potentiated the stimulatory effect of adenosine and CGS 21680 on IL 10 production , whereas IFN gamma treatment almost completely abolished this effect . ^^^ CGS 21680 stimulated an increase in intracellular cAMP in a time and dose dependent manner in IL 1 and TNF alpha treated cells but not in control or IFN gamma treated cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both in murine models and by study of Crohn disease itself , we have shown that Crohn inflammation is due to a Th 1 T cell abnormality involving overproduction of interleukin ( IL ) 12 , interferon ( IFN gamma , and tumor necrosis factor ( TNF ) alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In co infected patients cellular unresponsiveness to parasite antigens was observed , while cellular production of tumour necrosis factor alpha ( TNF alpha ) and gamma interferon ( IFN gamma ) was greater in those doubly infected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Staphylococcal Enterotoxin A ( SEA ) , a 27kDa monomeric protein , produced by some strains of Staphylococcus aureus , is a prototype T cell superantigen which causes proliferation of cytotoxic T lymphocytes and produces cytokines like TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Addition of TNF alpha enhanced the IFN gamma mediated antibacterial effects , whereas TNF alpha alone had no influence on staphylococcal growth . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Plasma IFN gamma and total TNF alpha concentrations increased during infection , but free TNF alpha levels were low in all patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In smokers , the percentage of bronchial epithelial ( BE ) cells and macrophages with high intracellular contents of the pro inflammatory cytokines IL 8 , IFN gamma and TNF alpha , were significantly increased in comparison with non smokers . ^^^ After smoking cessation , expression of IL 8 was significantly decreased by the 16th week , that of IFN gamma was significantly decreased by the 12th to the 16th week , but that of TNF alpha was unchanged even at 16 weeks post cessation , and that of IL 10 was significantly increased at that time . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In a prospective study , using enzyme linked immunospot ( ELISPOT ) assays , we examined numbers of blood mononuclear cells ( MNC ) secreting pro ( IFN gamma , TNF alpha , IL 6 ) and anti inflammatory ( IL 10 ) cytokines in patients with WAD and , for reference , patients with ankle sprain and multiple sclerosis and healthy subjects . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both HSV type 1 ( HSV 1 ) and HSV 2 induced TNF alpha expression in macrophages activated with gamma interferon ( IFN gamma ) . ^^^ Recombinant HSV 1 glycoprotein D was able to trigger TNF alpha secretion in concert with IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition to antimicrobial therapy , modifications of macrophage function by IFN gamma and blockade of TNF are attractive areas for therapeutic research . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Among polarized APC , effector macrophages are classically activated by mediators such as IFN gamma , TNF alpha or LPS ( M phi 1 ) , while M phi 2 are alternatively activated by IL 4 , IL 10 or PGE ( 2 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After human monocytes were cultured for 24 hours in serum free medium and granulocyte macrophage colony stimulating factor to produce immature DCs , additional overnight culture with either calcium ionophore ( CI ) or interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and soluble CD40L resulted in phenotypically mature DCs that produced interleukin 8 ( IL 8 ) and displayed marked expression of CD 80 , CD 86 , CD 40 , CD 54 , CD 83 , DC LAMP , and RelB . ^^^ DCs matured by IFN gamma , TNF alpha , and soluble CD40L were additionally distinguished by undetectable CD 4 expression , marked secretion of IL 12 , IL 6 , and MIP 1beta , and preferential ability to promote Th1 / Tc1 characteristics during T cell sensitization . ^^^ Calcium signaling selectively antagonized IL 12 production by mature DCs activated with IFN gamma , TNF alpha , and soluble CD40L . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effect of interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 beta ( IL 1beta ) , and H2O2 , on hepatitis B virus ( HBV ) replication was analyzed in the HBV DNA transfected human hepatoblastoma derived cell line HB 611 . ^^^ Secretion of HBV DNA from HB 611 cells was inhibited by IFN gamma , TNF alpha , IL 1beta , and H2O2 in a dose dependent manner . ^^^ The level of manganese SOD mRNA , indicative of occurrence of oxidative stress , increased immediately after treatment with IFN gamma , TNF alpha , IL 1beta , and H2O2 . ^^^ Our findings suggest that oxidative stress may be a common factor in the antiviral effects of IFN gamma , TNF alpha , and IL 1beta on HBV . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Peritoneal inflammatory macrophages , but not resident peritoneal macrophages , derived from HIV 1 transgenic mice and activated in vitro with LPS and IFN gamma ( or tumor necrosis factor alpha and IFN gamma ) also produced about 50 % less NO than did macrophages harvested from nontransgenic littermates . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The presence of IL 4 , IL 6 , IL 8 , IL 10 , RANTES , IFN gamma , TNF alpha , and NF kappa B and its inhibitor 1 kappa B alpha was qualitatively evaluated by immunofluorescent staining . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma and TNF alpha were able to upregulate surface Fas and FasL , whereas IL 1beta downregulated surface FasL . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Even more , while immunization with hsp 70 led to Ag induced production of IL 10 and IL 4 , immunization with aldolase led to increased production of IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lethal infections caused by type 1 ( RH ) or type 2 ( PTG ) strain infections were accompanied by extremely elevated levels of Th 1 cytokines in the serum , including IFN gamma , TNF alpha , IL 12 , and IL 18 . ^^^ Increased time of survival following lethal infection with the RH strain was provided by neutralization of IL 18 , but not TNF alpha or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Incubation of resting PBMCs with high dose leptin ( 250 ng / ml , 3 5 days ) induced proliferation of resting cultured PBMCs and their secretion of TNF alpha ( 5 fold ) , IL 6 ( 19 fold ) , and IFN gamma ( 2 . 5 fold ) , but had no effect on IL 4 secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When adoptively transferred into rheumatoid arthritis synovium SCID mouse chimeras , these CD 4 T cell clones enhanced the production of IFN gamma , IL 1beta , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Typically , IL 12 , TNF alpha and IFN gamma are produced in the spleen , and are transiently present in plasma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
On the other hand , CD56bright NK cells are superior in producing the proinflammatory cytokines IFN gamma ( 28 . 5 % vs . 20 . 8 % , p < 0 . 05 ) and TNF alpha ( 28 % vs . 15 . 8 % , p < 0 . 001 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Suppressor of cytokine signaling 3 ( SOCS 3 ) is a negative feedback regulator of IFN gamma signaling , shown up regulated in mouse bone marrow cells by the proinflammatory cytokines interleukin 1beta ( IL 1beta ) , tumor necrosis factor alpha ( TNF alpha ) , and IFN gamma . ^^^ IL 1beta and IFN gamma alone , or potentiated by TNF alpha , are cytotoxic to the insulin producing pancreatic beta cells and beta cell lines in vitro and suggested to contribute to the specific beta cell destruction in Type 1 diabetes mellitus ( T1DM ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Human breast microvascular endothelial cells ( HMVEC ) , human aortic endothelial cells ( HAEC ) , human umbilical vein endothelial cells ( HUVEC ) and canine aortic endothelial cells ( CAEC ) were stimulated with cytokines tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) . ^^^ RESULTS : The binding of anti Fc gamma R 2 was significantly increased by the simultaneous stimulation with TNF alpha and IFN gamma on all three types of human endothelial cells ( ECs ) , but not on canine endothelial cells . ^^^ CONCLUSIONS : This study demonstrate that cytokines TNF alpha and IFN gamma enhance low affinity Fc gamma R expression on human endothelial cells in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We characterized and compared the activity of NK and B cells and major pro inflammatory factors ( IFN gamma , TNF alpha ) in treated animals with and without toxicity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increased levels of IL 6 , TNF alpha , IFN gamma , IL 2 , IL 4 , IL 8 , IL 10 , and GM CSF were detected in infected animals , and the increase in circulating cytokines correlated with an increase in circulating viral antigen . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Up regulation of the IFN gamma / IL 10 ratio and TNF alpha was associated with poor renal function at the time of biopsy . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Unlike IFN gamma cocultured cells , FAT treated monocytes appeared not to have enhanced TNF alpha message stability , suggesting that de novo transcription may be involved in this effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To examine the potential contribution of chemokines and cytokines to the inflammatory cell recruitment in tissues involved by lymphoid interstitial pneumonitis from HIV infected pediatric patients , RNA was extracted from paraffin embedded tissues from five lung biopsies in four pediatric HIV positive patients and from five control , normal lung biopsies in five HIV negative patients and was analyzed by semiquantitative RT PCR for the expression of cytokines ( TNF alpha , GM CSF , IFN gamma , IL 4 , IL 6 , IL 10 , and IL 18 ) and chemokines ( IP 10 , Mig , regulated upon activation , normal T expressed and secreted [ RANTES ] , and MIP 1 alpha and beta ) after normalization for G3PDH . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interestingly , Ip 10 mRNA , although not basally detected in cultured TFCs , was strongly induced by IFN gamma and synergistically increased by TNF alpha addition . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inducible NO synthase ( iNOS ) was detected in cultured PBMC by immunofluorescence , immunoblotting and flow cytometry with or without treatment of the cells with interleukin ( IL ) 1beta+ tumour necrosis factor alpha ( TNF alpha ) , IL 4 or interferon gamma ( IFN gamma ) from day 1 to day 5 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF and IFN gamma of experimental animal could play important roles in regulating immune functions of schistosomiasis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Blockade of phosphodiesterase 4 with rolipram reduced the production of tumor necrosis factor ( TNF ) alpha , interleukin ( IL ) 5 , IL 10 , and IL 2 but poorly inhibited cell proliferation and interferon gamma ( IFN gamma ) production by activated human T cells . ^^^ Addition of dibutyryl cAMP mimicked rolipram inhibitions on proliferation , IL 2 , TNF alpha , and IFN gamma but not on IL 10 or IL 5 production . ^^^ Moreover , the inhibitory effects of rolipram on proliferation , IFN gamma , and TNF alpha but not of IL 10 production can be prevented by a specific protein kinase A inhibitor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In sera of cachectic patients bearing advanced cancers , the concentration of interleukin 1 ( IL 1 ) , interleukin 6 ( IL 6 ) , tumor necrotizing factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) have been reported to elevate . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The use of the VSL 3 probiotic mixture in the IL 10 gene deficient mouse resulted in a complete normalization of physiological transport function and barrier integrity , in conjunction with a reduction in mucosal secretion of TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Further , proinflammatory cytokines , such as tumor necrosis factor alpha ( TNF ) , interleukin 1beta ( IL 1beta ) , and interferon gamma ( IFN gamma ) , as well as chemokines , are synthesized by resident brain cells and T lymphocytes invade the affected brain tissue . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , the proportion of CD8+ cells co expressing TNF alpha ( 47+ / 5 vs . 33+ / 4 ; p=0 . 05 ) and both TNF alpha and IFN gamma was significantly higher in the patients than the controls ( 41+ / 6 vs . 22+ / 3 % ; p=0 . 04 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our results demonstrate that IFN gamma and IFN beta significantly inhibit MMP 9 enzymatic activity and protein expression that is induced by PMA and the cytokine TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These changes were independent of IL 12 , IFN gamma , and TNF alpha , as assessed in mice with targeted mutations . ^^^ Thus , following MCMV infection , NK cells accumulate at sites of viral replication in an IL 12 , IFN gamma , and TNF alpha independent manner . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The more effective control of bacteria in the IL 12p35 ( / ) mice was associated with elevated serum IFN gamma and TNF alpha levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we establish that TNF alpha and IFN gamma induce HA binding and the sulfation of CD 44 in CD 14 ( + ) PBMC , whereas no induced HA binding or CD 44 sulfation was observed in CD 14 ( ) PBMC stimulated with TNF alpha . ^^^ Treatment of cells with NaClO ( 3 ) , an inhibitor of sulfation , prevented HA binding in a significant percentage of CD 14 ( + ) PBMC induced by TNF alpha , LPS , IL 1beta , or IFN gamma . ^^^ Furthermore , stimulation with TNF alpha or IFN gamma in the presence of NaClO ( 3 ) reduced the ability of isolated CD44H to bind HA , demonstrating a direct effect of CD44H sulfation on HA binding . ^^^ Overall , these results demonstrate that inducible sulfation of CD44H is one mechanism used by CD 14 ( + ) peripheral blood monocytes to induce HA binding in response to inflammatory agents such as TNF alpha and IFN gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , expression of cytokines ( IFN gamma and TNF ) , chemokines ( IFN inducible protein 10 and RANTES ) , the immune accessory molecules , MHC class 2 , B7 . 2 , ICAM 1 and VCAM 1 , and NO synthase 2 was induced in the CNS of the GF IL 12 mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , all of the Thl type and Th 1 inducing cytokines tested [ IFN gamma , tumor necrosis factor ( TNF ) alpha , IL 12 and IL 18 ] were secreted by OK PSA stimulated PBMC far better than by Su PSA treated PBMC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma was almost undetectable and TNF alpha was detected at low levels . ^^^ Concentrations of IFN gamma and TNF alpha in whey from mastitic cattle decreased significantly as the cows recovered . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To determine the dynamics of intrahepatic cytokine expression and T cell involvement , and to assess their value in predicting the outcome of acute hepatitis ( AH ) in the adult onset of WHV infection , we evaluated liver transcription of interferon gamma ( IFN gamma ) ; tumor necrosis factor alpha ( TNF alpha ) ; interleukins ( IL ) 2 , 4 , and 6 ; and the T cell influx in relation to disease histologic severity and virus load in serial liver biopsies collected during the life span of experimentally infected woodchucks . ^^^ Our results show that recovery from viral AH in adulthood is preceded by a significantly greater hepatic expression of IFN gamma and CD 3 , an increased TNF alpha transcription , lower hepatic WHV load , and a greater degree of liver inflammation than those in acute infection with CH outcome . ^^^ Furthermore , we have learned that the elevated IFN gamma , TNF alpha , and CD 3 expression in the liver endures for years not only in CH , but also , although to a lesser extent , in apparently completely resolved infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Thyroid cells have been shown to induce class 2 expression ( mainly HLA DR ) following stimulation with IFN gamma and addition of tumor necrosis factor ( TNF ) alpha synergistically enhanced this expression . ^^^ The aim of this study was to find whether CIITA is synergistically induced by IFN gamma and TNF alpha in the human thyroid MRO 87 1 cell line , and to investigate the molecular mechanisms responsible for this synergism . ^^^ We have demonstrated that IFN gamma and TNF alpha synergistically induce HLA DRalpha and CIITA mRNAs , but prolonged incubation resulted in the inhibition of CIITA mRNA accumulation . ^^^ Increased binding of IFN regulating factor 1 mediates the synergistic induction of CIITA by IFN gamma and tumor necrosis factor alpha in human thyroid carcinoma cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here , in the course of trying to understand the mechanism for synergy between IL 12 and TNF alpha in up regulating IFN gamma production , we find that when the stimulus through the TCR is too weak to induce cell proliferation , which would be needed for selection , IL 12 and TNF alpha synergize to up regulate not only IFN gamma , but also the IL 12Rbeta2 chain , which triggers IFN gamma production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PECAM 1 was constitutively expressed at a high level on HUVEC but its expression level decreased following stimulation by tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) . ^^^ PECAM 1 was also constitutively expressed on microvascular endothelial cells MvE and GEC , but at lower levels than on HUVEC , and expression by these cells also decreased in response to TNF alpha and IFN gamma . ^^^ TNF alpha induced the expression of VCAM 1 on HUVEC and GEC , but not MvE , while IFN gamma induced VCAM 1 expression only on HUVEC . ^^^ TNF alpha induced the expression of E selectin on all three kinds of endothelial cells , but IFN gamma had no effect on E selectin expression . ^^^ GEC therefore showed expression patterns of PECAM 1 , VCAM 1 , and E selectin different from those seen in HUVEC and MvE upon treatment with TNF alpha or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha , IL 8 , and IFN gamma appeared to correlate with CMV antigenemia . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We measured the secretion of interleukin ( IL ) 1beta , IL 6 and tumor necrosis factor alpha ( TNF alpha ) from human monocytic ( THP 1 ) , astrocytic ( U 373 MG ) and neuronal ( SH SY5Y ) cell lines alone and in co culture , with and without stimulation by a combination of lipopolysaccharide ( LPS ) plus interferon gamma ( IFN gamma ) or amyloid beta peptide 1 40 ( Abeta ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Polymorphisms in interleukin ( IL ) 2 , IL 6 , IL 10 , tumor necrosis factor ( TNF alpha ) , tumor growth factor ( TGF beta ) , and interferon ( IFN gamma ) were determined by polymerase chain reaction ( PCR ) . ^^^ RESULTS : Statistical difference between protein production and cytokine polymorphic variants in the IL 10 , IFN gamma , and TNF alpha genes was not evident after 48 hour stimulation with concanavalin A . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In a study of hospitalized patients in Malawi , we compared cytometrically assessed , cell specific cytokine data to serum interleukin 2 ( IL 2 ) , IL 4 , IL 6 , IL 8 , IL 10 , gamma interferon ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) levels in 16 children and 71 ( IL 2 , 4 , 6 , 10 ) or 159 ( IL 8 , IFN gamma , and TNF alpha ) adults , using Wilcoxon rank sum tests and Pearson ' s ( r ( p ) ) and Spearman ' s ( r ( s ) ) rank correlations . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although it has been well known that the role of LPS on hepatotoxicity is mediated through TNF alpha , the direct cytotoxic effect of LPS on IFN gamma primed hepatocytes has not yet been clearly demonstrated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We evaluated in this cross sectional study whether IL 18 , IL 12p35 , IL 12p40 , TNF alpha , IFN gamma , IL 10 , IL 4 , TGF beta , IL 12Rbeta1 , and IL 12Rbeta2 mRNA expression in unstimulated white blood cells showed significant differences between relapsing remitting ( RR ) , secondary progressive ( SP ) and PP MS patients , and healthy controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OBJECTIVES : The impact of intrahepatic messenger RNA ( mRNA ) levels of interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) on the outcome of antiviral treatment of chronic hepatitis C was evaluated . ^^^ In sustained responders , pretreatment intrahepatic mRNA levels of IFN gamma and TNF alpha were lower than in nonsustained responders ( IFN gamma , 0 . 23 + / 0 . 10 vs . 0 . 35 + / 0 . 07 , respectively ; p = 0 . 024 and TNF alpha , 1 . 2 + / 0 . 7 vs . 2 . 3 + / 1 . 4 , respectively ; p= 0 . 009 ) ; similarly , HCV viral load was lower in sustained responders than in nonresponders ( 663 , 424 + / 756 , 389 copies / mL vs . 1 , 656 , 713 + / 1 , 517 , 683 copies / mL , respectively ; p = 0 . 037 ) . ^^^ CONCLUSIONS : Higher intrahepatic mRNA levels of IFN gamma and TNF alpha may reflect interferon resistance of HCV strains and may contribute to tissue damage in patients refractory to antiviral treatment . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Flow cytometric analysis indicated that CD8+ T cells from both HIV+ and aged donors showed an increase of approximately 2 3 fold over controls in percentage of cells producing inflammatory cytokines IFN gamma and TNF alpha . ^^^ Quantitative PCR also showed a substantial increase ( 4 5 fold ) in IFN gamma and TNF alpha mRNA from HIV+ and aged CD8+ T cells , as did ELISA for secreted IFN gamma and TNF alpha ( 2 . 3 4 fold ) . ^^^ Flow cytometric analysis showed that the CD8+ CD 28 T cell subset accounts for approximately 80 86 % of the IFN gamma and TNF alpha production from the CD8+ subset in the aged and HIV+ states . ^^^ The CD4+ T cell , while not significantly changed in the HIV+ or aged states in terms of IFN gamma production , showed a small but significant increase in TNF alpha production in both states . ^^^ CONCLUSIONS : Our data appear compatible with physiologic conditions existing in HIV+ and aged individuals , i . e . elevated serum levels and elevated CD8+ T cell production of IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor macrophages express cell surface associated TNF , TNF type 1 ( CD120a ) and 2 ( CD120b ) receptors , and , upon contact with T cells which induces release of IFN gamma from T cells , secrete nitric oxide . ^^^ Killing of T cells in vitro is blocked by Abs to IFN gamma , TNF , CD120a , or CD120b , or N methyl L arginine . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
No significant decline in cross priming against OVA was measured when either TNF or IFN gamma was neutralized in L . monocytogenes infected animals , demonstrating that multiple signals exist to overcome CD40L blockade of CD 8 ( + ) T cell cross priming during bacterial infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The evaluation of a cytokine pattern produced by the responding CD 4 ( + ) T lymphocytes revealed that CPSs induce a dominant Th 2 pattern , with high levels of IL 4 and IL 10 production and undetectable inflammatory cytokines , such as TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increased levels of Fc alpha RI on blood phagocytes correlated with enhanced serum IL 6 levels , but not with IFN gamma or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although TNF alpha and IFN gamma are known to be important factors for the development of the disease , the mechanism of their overproduction has not been clarified , yet . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this cell line , THR ( 1 10 U / mL ) and PAR 1 AP ( 1 100 microM ) induced a significant concentration dependent increase both of IFN gamma ( 250 U / mL ) or TNF alpha ( 500 U / mL ) induced NO release . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The production of TNF alpha , IFN gamma , IL 10 , and IL 13 was measured in supernatant after 1 , 2 , 3 , 4 , and 7 days of cell culture . ^^^ In cell culture , stimulated PBMC of rejectors were found to produce significantly higher levels of TNF alpha , while there was a trend towards higher production of IFN gamma and IL 10 as compared to nonrejectors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Resveratrol also inhibited the production of IFN gamma and IL 2 by splenic lymphocytes , and the production of TNF alpha and IL 12 by peritoneal macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Chemical sympathectomy by 6 OHDA upregulated interleukin 12 ( IL 12 ) and tumor necrosis factor alpha ( TNF alpha ) production in enriched dendritic cell cultures and gamma interferon ( IFN gamma ) and TNF alpha production in spleen cell cultures , whereas chemical sympathectomy had no apparent effect on phagocytic activities , listericidal activities , and nitric oxide production in peritoneal exudate cells and splenic macrophages . ^^^ Augmentation of host resistance against L . monocytogenes infection by 6 OHDA was abrogated in IFN gamma ( / ) or TNF alpha ( / ) mice , suggesting that upregulation of IFN gamma , IL 12 , and TNF alpha production may be involved in 6 OHDA mediated augmentation of antilisterial resistance . ^^^ These results suggest that the sympathetic nervous system may modulate host resistance against L . monocytogenes infection through regulation of production of IFN gamma , IL 12 , and TNF alpha , which are critical in antilisterial resistance . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , the clinical isolate induces a significantly higher and more prolonged MPhi activation , as shown by reverse transcription PCR analysis of IL 1beta , IL 6 , IL 10 , transforming growth factor beta , tumor necrosis factor alpha , and gamma interferon ( IFN gamma ) transcripts . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma production was proportional to tumor necrosis factor alpha and interleukin 10 ( IL 10 ) levels but did not correlate with IL 5 production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation with gamma interferon ( IFN gamma ) and / or tumor necrosis factor ( TNF ) did not enable neurons to inhibit parasite invasion and replication . ^^^ Neuronal expression of some cytokines ( IL 6 , TGF beta 1 ) and chemokines ( MIP 1beta ) was regulated by infection and / or by IFN gamma and TNF . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Recipients were treated with neutralizing antibodies against tumor necrosis factor ( TNF ) , interferon gamma ( IFN gamma ) , or interleukin 1 ( IL 1 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Highly sensitive determination of plasma cytokines by time resolved fluoroimmunoassay ; effect of bicycle exercise on plasma level of interleukin 1 alpha ( IL 1 alpha ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) . ^^^ IL 1 alpha , TNF alpha , and interferon gamma ( IFN gamma ) were about 1 / 10 smaller than those of enzyme linked immunosorbent assay currently used . ^^^ Blood samples were taken before , during , and after exercises , and the concentration levels of plasma IL 1 alpha , TNF alpha , and IFN gamma were determined . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The weak TNF alpha response combined with the vigorous IL 6 and IL 12 response to ODN 2059 indicate the potential use of this CpG ODN as an adjuvant to enhance both antibody mediated and IFN gamma mediated macrophage activation , which are important for protection against disease caused by intracellular pathogens of cattle . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
One of the mechanisms by which beta cells are killed in type 1 diabetes is by the release of the cytokines interleukin 1beta ( IL 1beta ) , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) by immune cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , serum and spleen cell IFN gamma levels in infected , gene deleted mice were reduced 60 75 % relative to infected normal animals , and spleen cell TNF alpha was likewise reduced by approximately 50 % . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increased gene expression of IFN gamma in several tissues and elevated serum TNF alpha were also noted . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Prior studies have suggested that immune reactants play a role in the pathogenesis of atherosclerosis ; we report here that immune reactants , IFN gamma and immune complexes bound to C1q , but not interleukin 1 and tumor necrosis factor , diminish the expression of cholesterol 27 hydroxylase in human aortic endothelial cells , peripheral blood mononuclear cells , monocyte derived macrophages , and the human monocytoid cell line THP 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
At follow up , fatigue was associated with IFN gamma ( > or =7 pg / ml ) and / or TNF alpha ( > or =40 pg / ml ) ( P=0 . 0275 ) . ^^^ Prolonged upregulation of serum IFN gamma and TNF alpha appears to represent a consistent host response to symptomatic B 19 virus infection . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha , IL 6 and IFN gamma secreted by bronchoalveolar leukocytes isolated from patients with bronchial asthma , complicated by fungal airways infections . ^^^ The activity of TNF alpha , IL 6 and IFN gamma were measured in the BAL cell culture supernatants by using specific bioassays . ^^^ Nevertheless , fungal airways infections may lead to increase of LPS or PHA induced production of TNF alpha , IL 6 or IFN gamma ( despite prednisone therapy ) by prestimulation of the BAL cells with fungi . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In cells , SP 600125 dose dependently inhibited the phosphorylation of c Jun , the expression of inflammatory genes COX 2 , IL 2 , IFN gamma , TNF alpha , and prevented the activation and differentiation of primary human CD 4 cell cultures . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To evaluate this , human lung fibroblasts were cast into native type 1 collagen gels and floated in medium containing cytomix ( TNF alpha , IL 1beta , and IFN gamma ) alone or in combination with NE in the presence and absence of retinoic acid ( 1 microM ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human 5 gamma 2V delta 2 T cells produce IFN gamma and TNF alpha with an on / off / on cycling pattern in response to live bacterial products . ^^^ In this study , we demonstrate that human Vgamma2Vdelta2 T cells produce IFN gamma and TNF alpha as early as 2 h after Ag exposure , and that they produce these cytokines in a dose and time dependent manner in response to stimulation with a live bacterial product , iso butylamine ( IBA ) , but not to dead bacteria or LPS . gammadelta T cells began , ceased , and then resumed IFN gamma and TNF alpha generation in an on / off / on cycling pattern , both in vitro and in vivo , depending on the presence or absence of IBA . ^^^ IFN gamma and TNF alpha , whose optimum production was dependent on IBA stimulated gammadelta T cells , were critical for monocyte mediated killing of Escherichia coli . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It is well established that cytokines such as interleukin 1beta ( IL 1beta ) , tumor necrosis factor alpha ( TNF alpha ) , and gamma interferon ( IFN gamma ) inhibit beta cell function and are cytotoxic to human and rodent pancreatic islets in vitro . ^^^ Furthermore , IL 1beta stimulation of JNK activity is specific , because TNF alpha and IFN gamma were without effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The purpose of this study was to investigate the time course of release of the cytokines / chemokines interleukins ( IL ) IL 1beta , IL 1ra , tumour necrosis factor alpha ( TNF alpha ) , IL 6 , IL 8 , interferon gamma ( IFN gamma ) and monocyte chemotactic protein ( MCP 1 ) , soluble intercellular adhesion molecule 1 ( sICAM 1 ) , myeloperoxidase ( MPO ) and bradykinin in nasal secretions of patients with a naturally acquired URTI . ^^^ Compared to baseline , significantly elevated concentrations of total protein , bradykinin , IL 1beta , TNF alpha , IL 6 , IL 8 , MCP 1 , IFN gamma , MPO and sICAM 1 were detected in nasal lavage fluids of symptomatic patients , whereas IL 1ra remained unaltered . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Randomized study of antibodies to IFN gamma and TNF alpha in secondary progressive multiple sclerosis . ^^^ In a double blind , placebo controlled trial , 45 patients with active secondary progressive MS were randomized to three groups of 15 patients , each receiving a short course of antibodies to IFN gamma , to tumor necrosis factor ( TNF ) alpha , or a placebo . ^^^ This was supported by MRI data ( a decrease in the number of active lesions ) and systemic changes in cytokine status ( a decrease in IL 1beta , TNF alpha , and IFN gamma concentrations in supernatants of actvated blood cells of these MS patients and an increase in TGF beta production ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lymphocytes taken from CD200Fc treated , collagen immunized mice produced significantly lower levels of TNFalpha and IFN gamma in culture supernatants after restimulation in vitro with collagen , in contrast to cells taken from control mice treated with PBS or normal mouse Ig . ^^^ Serum from CD200Fc treated mice contained less anti collagen IgG ( approximately 50 % reduction ) , with relatively more IgG2b and IgG 3 , and lower levels of TNFalpha and IFN gamma , than control mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In Crohn ' s disease , intestinal lamina propria ( LP ) T cells overproduce TNF alpha and IFN gamma , and clinical and animal studies indicate that this is pathogenic . ^^^ We tested three analogues [ selective cytokine inhibitory drugs ( SelCIDs ) A , B , and C , all potent PDE 4 inhibitors ] for effect on TNF alpha , IFN gamma , and IL 10 production by and on proliferation of intestinal LP mononuclear cells after T cell stimulation and results were compared with those for peripheral blood leukocytes ( PBL ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When the inflammation was severest , TNF alpha , IL 1alpha , IL 10 , IFN gamma and iNOS mRNA increased to their peak , which varied with the degree of induction and different time course . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Levels of IL 1 beta , TNF alpha , IL 10 , IL 12 , and IFN gamma were measured by immunoenzymatic methods . ^^^ Higher levels of the proinflammatory cytokines IL 1 beta , TNF alpha , and IL 6 were found in AOD ( P < 0 . 05 ) . mRNA levels of IL 12 and IFN gamma did not differ between the diseases . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : MIN 6 cells were cultured in the presence of IL 1 beta , TNFalpha , and / or IFN gamma . ^^^ NO production was stimulated by IL 1 beta and augmented by TNFalpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The percentages of CD 8 ( + ) T cells spontaneously producing IL 6 and of all lymphocytes producing induced TNF alpha and IFN gamma in the same cell had the strongest relationships to iron deficiency ( b = + 0 . 0211 , P = 0 . 005 and b = + 0 . 1158 , P = 0 . 012 , respectively , LRA ) and were also positively related to the co expression of the T cell activation markers HLA DR and CD 38 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma synergizes with TNF alpha but not with viable H . pylori in up regulating CXC chemokine secretion in gastric epithelial cells . ^^^ Human gastric epithelial cell lines ( AGS , Kato 3 , NCI ) were used to characterize the constitutive and regulated expression of three CXC chemokines in response to IFN gamma , TNF alpha and different H . pylori preparations . ^^^ IFN gamma in combination with TNF alpha strongly induced secretion of those chemokines . ^^^ Soluble or membranous fractions of H . pylori significantly inhibited IFN gamma / TNF alpha induced epithelial cell IP 10 and Mig production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Administration of cDNA encoding soluble IFN gamma receptor ( IFN gamma R ) / IgG Fc fusion proteins , soluble TNF alpha receptors , or IL 1 receptor antagonist ( IL 1ra ) , protects against either lupus , various forms of arthritis , autoimmune diabetes , or other autoimmune diseases . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We then applied this technique to determine the kinetics of IL 1ra , IL 1beta , IL 5 , IL 13 , TNF alpha and IFN gamma induction upon stimulation of human peripheral blood mononuclear cells ( PBMC ) by phytohaemagglutinin ( PHA ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In situ hybridization was used to detect cells expressing interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) and interleukin 4 ( IL 4 ) mRNAs in orf skin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A new methodology based on single tube RT PCR has been developed to determine the expression of multiple canine cytokines ( TNF alpha , IL 2 , IFN gamma , IL 18 , IL 4 , IL 6 and IL 10 ) using primers and protocols designed allow specific amplification of the mRNAs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The current results confirm that culture of human umbilical vein endothelial cells with TNF alpha plus IFN gamma caused a decrease in surface expressed and junctional localized JAM and PECAM 1 , but did not cause decreased leukocyte transmigration in an in vitro flow assay . ^^^ IFN gamma alone or TNF alpha plus IFN gamma significantly increased permeability , but TNF alpha alone did not , suggesting lack of correlation between transmigration and loss of permeability . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Previous studies have demonstrated a role for IL 12 , IFN gamma , and TNF alpha in the development of murine SGVHD . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TCCM prepared from cell free supernatants of anti CD 3 activated T cells contained several soluble factors including CD 40 ligand ( sCD40L ) , TNF alpha , and IFN gamma . ^^^ The ability of TCCM to induce such phenotypic changes could be abrogated by neutralizing antibodies specific for CD40L , TNF alpha , and IFN gamma , indicating that these factors present in TCCM are mainly implicated in the maturation of DC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma , in concert with tumor necrosis factor alpha ( TNF alpha ) , leads to a remarkable reduction of intrahepatic replication intermediates and specific mRNAs of hepatitis B virus ( HBV ) by a noncytolytic mechanism in the transgenic mouse model . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This indicates that only the cross talk between TNF alpha and the IFN beta pathways , and not IFN alpha / beta and IFN gamma signaling in general , is perturbed in cervical carcinoma cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines which were able to replace LPS in priming for LcV in the Shw r ( TNF alpha and IL 1beta ) also induced sustained expression of adhesion molecules , whereas IL 12 and IFN gamma did neither . ^^^ Neutralizing IL 12 or IFN gamma also inhibited neither LcV nor sustained expression of adhesion molecules , whereas anti TNF alpha inhibited both . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , an in vivo blockade of TNF alpha preferentially inhibited the production of IFN gamma and blocked IFN gamma dependent up regulation of Fas ; however , cytokines such as IL 10 , IL 6 , or IL 4 were not inhibited . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Intratracheal administration of rat recombinant IL 18 in the lung model caused significant increases in lung vascular permeability and in BAL content of neutrophils and in BAL content of TNF alpha , IL 1beta , and cytokine induced neutrophil chemoattractant , whereas intratracheal instillation of anti IL 18 greatly reduced these changes and prevented increases in BAL content of IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The addition of DHEA to cultures of myelin basic protein primed splenocytes resulted in a significant decrease in T cell proliferation and secretion of ( pro ) inflammatory cytokines ( IFN gamma , IL 12 p 40 , and TNF alpha ) and NO in response to myelin basic protein . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These responses were associated with Ag specific IFN gamma and / or TNF alpha secretion , but not IL 4 , consistent with induction of Th 1 immunity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To study the potential role of cytokines in the spinal cord of AIA , we wanted to determine whether there are changes of glial and cytokine expression ( IL 1 beta , IL 6 , TNF alpha and IFN gamma ) in the spinal cord of AIA rats . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
AIM : To study the effects of cimetidine ( Cim ) on the production of interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) by splenocytes in immune derived aplastic anemic ( AA ) mice . ^^^ METHODS : Aplastic anemic mice model was constructed first , and then the splenocytes were induced to secrete IFN gamma and TNF alpha . ^^^ Concentration of IFN gamma was assayed using sandwich ELISA , while that of TNF alpha was measured with L 929 cytotoxicity methods . ^^^ RESULTS : ( 1 ) Concentrations of IFN gamma and TNF alpha secreted by splenocytes from AA mice were ( 137 + / 36 ) ng / L and ( 6 + / 3 ) microg / L , respectively , much more than the irradiated and the control mice . ( 2 ) Treatment with Cim 10 micromol / L reduced the concentrations of IFN gamma and TNF alpha to ( 14 + / 8 ) ng / L and ( 2 . 7 + / 0 . 6 ) microg / L , respectively . ^^^ CONCLUSION : Cim could effectively reduce the production of IFN gamma and TNF alpha from splenocytes of AA mice . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Fewer levels of CD 69 positive cells after 4 h mitogenic activation , altered expression of cytokines ( IL 2 , IFN gamma and TNF alpha ; 22 h ) and lower proliferation ( 72 h ) were determined in aging . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
During cell mediated immune response , increased amounts of interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) are released . ^^^ TNF alpha alone and TNF alpha in combination with IFN gamma led to no alteration in cell viability during 48 h of incubation . ^^^ The combination of TNF alpha and IFN gamma almost completely abrogated the rate of apoptosis induced by NH 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin 18 ( IL 18 ) , a pleiotropic cytokine produced by activated macrophages , plays significant roles in the immune response , inducing the secretion of IFN gamma , TNF alpha and IL 2 , enhancing NK cell activity and potentiating the differentiation of Th 1 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Blood samples for analysis of tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , interleukin 1gamma ( IL 1gamma ) , interleukin 6 ( IL 6 ) , interleukin 8 ( IL 8 ) , interleukin 10 ( IL 10 ) and interleukin 1 receptor antagonist ( IL 1ra ) were obtained prior to treatment and subsequently at 12 , 36 and 60 hr . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neonatal rat Schwann cells were incubated in vitro with 1 mM 8 Bromo cAMP ( 8 Br cAMP ) with or without the addition of interleukin 1 alpha ( IL 1 alpha ) , IL 1 beta , IL 2 , IL 6 , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , or transforming growth factor beta ( TGF beta ) . ^^^ Cells treated with IFN gamma or TNF alpha alone were more bipolar and more evenly distributed on coverslips than were control cells , whereas TGF beta alone induced elongated cells often in a swirling pattern . ^^^ TNF alpha , IFN gamma , and TGF beta inhibited the 8 Br cAMP induced morphologic changes , as well as the upregulation of Gal and downregulation of NGFRp 75 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF peptide / MCMV infected BALB / c and C57BL / 6 mice showed distinct differences in the expression pattern of IFN gamma , IL 10 , and TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro , neutralizing IL 18 resulted in a significant inhibition of TNF alpha , IL 6 , and IFN gamma secretion by macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Priming under Th 1 conditions induced a Th 1 profile characterized by increased secretion of IFN gamma and TNF alpha , while Th 2 conditions caused increased production of IL 4 ( Th 2 profile ) by the gamma delta T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
M isolated from EtOH fed mice did not respond as well to IFN gamma as M isolated from control mice as measured by control of S . typhimurium , as well as tumor necrosis factor ( TNF ) and nitric oxide production . ^^^ Activation of M from EtOH fed mice with LPS and IFN gamma produced levels of nitric oxide and TNF only slightly less than the levels seen in M from control mice , but a significant decrease in IL 6 was seen when M from EtOH fed mice were stimulated with this combination . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
BACKGROUND : Among the different factors playing crucial roles in endothelial cell activation , cytokines such as interleukin 1 ( IL 1 ) , interleukin 6 ( IL 6 ) , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) have been reported to demonstrate profound effects on this cell type . ^^^ IL 1beta , IL 2 , IL 4 , IL 6 , IL 10 , TNF alpha and IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It is shown here , by use of cultures of primary keratinocytes from wild type and PPAR beta / delta ( / ) mice , that such signals including TNF alpha and IFN gamma , induce keratinocyte differentiation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CTL from monkeys infected with nonpathogenic isolates of simian and simian human immunodeficiency virus expressed high levels of IFN gamma , TNF alpha , and IL 2 after in vitro exposure to a nonspecific mitogen or the optimal peptide representing a dominant virus specific CTL epitope . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IP 10 and Mig mRNA and protein were also expressed by primary cultures of human mesangial cells and human visceral epithelial cells after stimulation with IFN gamma or with IFN gamma plus tumor necrosis factor alpha ( TNF alpha ) ( which produced greater stimulation ) . ^^^ The induction of IP 10 and Mig mRNA and protein expression by IFN gamma plus TNF alpha was strongly inhibited by nitric oxide ( NO ) donors , such as sodium nitroprusside or S nitroso N acetylpenicillamine , but not by cGMP analogues . ^^^ Electrophoretic mobility shift assays demonstrated that NO donors repressed IP 10 gene transcription induced by IFN gamma plus TNF alpha through the inhibition of NF kappaB activation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Peripheral blood mononuclear cells stimulated with IgA 1 protease produce IFN gamma and tumor necrosis factor alpha but no , or very low amounts of , interleukin 10 ( IL 10 ) or IL 4 , indicating a Th 1 based proinflammatory immune response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of bioactive IL 12 primarily upregulated transcript levels of IFN inducible protein 10 ( IP 10 ) , RANTES , IFN gamma , and TNF alpha in the pancreas . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the previous study , we demonstrated that interleukin ( IL ) 18 up regulated intercellular adhesion molecule 1 ( ICAM 1 ) expression on monocytes in human peripheral blood mononuclear cells ( PBMC ) and that heterotypic interaction between monocytes / T or NK cells through ICAM 1 / LFA 1 intensified the production of IL 12 , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) in PBMC . ^^^ In the present study , we demonstrate that histamine inhibited the ICAM 1 expression in monocytes induced by IL 18 using flow cytometry and that the responses of IL 12 , IFN gamma , and TNF alpha induced by IL 18 were concentration dependently inhibited by coexisting histamine , whereas IL 18 inhibited IL 10 production was reversed by the same concentrations of histamine . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The administration of the pro inflammatory cytokines tumor necrosis factor alpha ( TNF alpha ) , interleukin 1beta ( IL 1beta ) , and interferon gamma ( IFN gamma ) directly into the lateral ventricle emulated the effects of LPS on CYP1A activity only in the brain . ^^^ Significant levels of several cytokines ( TNF alpha , IL 1beta , and IFN gamma ) were produced in the serum of animals following intracerebroventricular ( i . c . v . ) administration of LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In animal models , protective roles have been shown for IL 1alpha , TNFalpha , IFN gamma , IL 12 , IL 18 and IL 15 , whereas IL 4 and IL 10 inhibit host defenses against Salmonella . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of the treatment were evaluated by endocytoscopy , pathological examinations and ELISA assays for the levels of cytokines IL 2 , TNF alpha and IFN gamma in the patients ' serum and urine . ^^^ The levels of cytokines IL 2 , TNF alpha and IFN gamma in serum and urine increased significantly , especially the level of TNF alpha in urine . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The local expression of IL 1 beta , IL 2 , CD 25 , IL 4 , IL 5 , IL 6 , IL 10 , TNF alpha , IFN gamma was examined at 1 , 3 , 5 , 7 , 9 , 11 , 14 days after operation by reverse transcription polymerase chain reaction ( RT PCR ) . ^^^ The expression of IL 1 beta , IL 4 , IL 10 , IFN gamma was up regulated , and the expression of IL 2 , CD 25 , IL 5 , IL 6 , TNF alpha was significantly inhibited in group A . ^^^ The expression of IL 1 beta , IL 5 , IL 6 , IL 10 , IFN gamma was up regulated , and the expression of IL 2 , IL 4 , TNF alpha was significantly down regulated in group B . ^^^ The expression of IL 1 beta , IL 2 , CD 25 , IL 5 , TNF alpha , IFN gamma was up regulated , and the expression of IL 4 , IL 6 , Il 10 was significantly down regulated in group C . ^^^ The expression of IL 4 , IL 5 , IL 6 , IL 10 was up regulated , and the expression of IL 1 beta , IL 2 , CD 25 , IFN gamma , TNF alpha was significantly down regulated in group D . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
LPS ) have been shown to stimulate innate immunity responses ( e . g . production of cytokines such as IL 1 , IL 6 , IL 10 , TNF alpha and IFN gamma by macrophages ) . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A whole blood method for measurement of intracellular TNF alpha , IFN gamma and IL 2 expression in stimulated CD3+ lymphocytes : differences between young and elderly subjects . ^^^ The percentages of CD3+ cells producing TNF alpha and IFN gamma were significantly higher in elderly compared to young people ( p=0 . 0049 ; p=0 . 0026 , respectively ) after stimulation with PMA and ionomycin . ^^^ CD3+ cells expressing TNF alpha were highly associated with CD3+ cells expressing IFN gamma in both elderly and young people . ^^^ In contrast , IL 2 secreting CD3+ cells were associated with TNF alpha and IFN gamma producing CD3+ cells in young but not elderly subjects providing further evidence for the remodelling of the cytokine network associated with old age . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate the function of Th 1 ( IFN gamma , TNF alpha ) and Th 2 ( IL 4 , IL 10 ) cytokine balance in the maternal fetal tolerance . ^^^ METHODS : The IFN gamma , TNF alpha , IL 4 and IL 10 mRNA were studied from PBMCs of 11 normal non pregnancy control , 10 women in first trimester of normal pregnancy , 11 women in last trimester of normal pregnancy by semi quantitative reverse transcriptional polymerase chain reaction ( RT PCR ) method . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The antitumor effect was dependent on the CpG dinulceotide and correlated with the ability to stimulate serum Th 1 cytokines ( TNF alpha , IL 12 , and IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Concentrations of tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , interleukin 10 ( IL 10 ) and soluble tumor necrosis factor receptor ( sTNF Rp 75 ) were found to be significantly elevated in patients when compared to normal controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Other causes are : decreased erythrocyte survival ( from 120 days to 70 80 days ) , chronic blood loss ( mainly gastrointestinal and gynecological ) , inhibitors of erythropoiesis , inflammatory cytokines ( IL 1 , TNF alpha , IFN gamma ) and malnutrition ( folic acid , L carnitine and vitamin B 12 deficiency ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , innovative immunobiologicals ( PEG TNFRI , PEG TNF alpha antibody fragments , soluble TNFRI , CTLA 4 Ig , CD 40 ligand antibody , antibodies against IFN gamma , IL 6 , IL 12 , IL 15 , IL 18 , complements ) , inhibitors of TNF alpha translation ( peptides , anti sense constructs ) or TNF alpha synthesis ( targeting NF kappa B , p 38 MAP kinase , phosphodiesterase 4 , TNF alpha converting enzyme ) are forthcoming . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lymph node cells ( LNCs ) from these AA and OIA rats showed increased mRNA expression of IFN gamma , IL 2 and TNF alpha but not IL 4 . ^^^ LNCs from IFA immunized LEW rats showed increased expression of IL 4 , reduced expression of IFN gamma and TNF alpha and no IL 2 , in contrast to IFA immunized DA rats . ^^^ The pretreatment of IFA before CFA challenge could completely prevent AA in LEW rats and their LNCs showed increased expression of IL 4 and IFN gamma but not IL 2 and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 4 and IL 10 inhibition of IFN gamma and TNF alpha dependent nitric oxide production from bovine mononuclear phagocytes exposed to Babesia bovis merozoites . ^^^ The requirement for IFN gamma and / or TNF alpha as co stimulants with Babesia bovis merozoites for nitric oxide ( NO ) production was examined , as well as the regulatory role of IL 4 and IL 10 . ^^^ Purified B . bovis merozoites did not induce the production of NO in undifferentiated monocytes without addition of exogenous IFN gamma and TNF alpha unless the monocytes taken ex vivo were producing TNF alpha endogenously . ^^^ However , while merozoites plus IFN gamma induced TNF alpha mRNA expression in MDM , NO was not produced . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In aged mice fed an XL . casei strain Shirota diet , potent induction of gamma interferon ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) , which play a very important role in excluding IFV , was evident in nasal lymphocytes . ^^^ IFN gamma and TNF alpha production increased 12 and 3 . 5 fold , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Fractalkine is expressed by smooth muscle cells in response to IFN gamma and TNF alpha and is modulated by metalloproteinase activity . ^^^ In this study we demonstrate that IFN gamma and TNF alpha , but not IL 1beta , cooperate synergistically to induce fractalkine mRNA and protein expression in cultured aortic SMC . ^^^ Hence , during vascular inflammation , the synergistic induction of fractalkine by IFN gamma and TNF alpha together with its metalloproteinase mediated cleavage may finely control the recruitment of monocytes to SMC within the blood vessel wall . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , the efficacy of rhIL 10 to down regulate IFN gamma and especially TNF production in cell cultures from the involved tissues of Crohn ' s disease patients was poor , and the use of an anti IL 10R mAb even provided evidence for proinflammatory effects of IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Relationship between cell kinetics and apoptotic effects in TNF alpha and IFN gamma treated human tumour cell lines . ^^^ In this report , the effects of tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) on human tumour cell lines with respect to proliferation and apoptosis are examined . ^^^ Combined treatment with TNF alpha and IFN gamma augmented both the effects on the cell cycle and on apoptosis in the sensitive lines , and had only a minor additional effect on the resistant cell line as compared to single TNF alpha treatment . ^^^ Our data revealed a good linear correlation indicating that high levels of c Myc render cells sensitive to apoptosis , independent of the treatment , TNF alpha alone or TNF alpha in combination with IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Proliferation of tumor cells was detected by MTT method , tumor necrosis factor alpha ( TNF alpha ) was detected by biological assay , and interferon gamma ( IFN gamma ) by enzyme linked immunosorbent assay ( ELISA ) . ^^^ GL B significantly increased TNF alpha and IFN gamma release and their mRNA expression in the cultured medium . ^^^ CONCLUSION : The anti tumor activity of GL B was derived from promoting mRNA expression of TNF alpha and IFN gamma , resulting in TNF alpha and IFN gamma release . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We postulate that in addition to higher IFN gamma and TNF alpha production , resistance to early apoptosis of the invading cells in the target tissue possibly due to lack of downregulation by TGF beta leads to exceptional susceptibility to EAE in DA rats . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Microflora reactive IL 10 producing regulatory T cells are present in the colon of IL 2 deficient mice but lack efficacious inhibition of IFN gamma and TNF alpha production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha , IFN gamma , IL 10 , IL 12 , and IL 18 were found at higher levels during the ischemic time , whereas IL 8 predominantly increased after reperfusion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Specifically , stress preconditioning decreased the production of nitrite by endotoxin stimulated alveolar macrophages removed from haemorrhaged rats or by A 549 and rat alveolar epithelial type 2 cell monolayers stimulated with cytomix ( a mixture of TNF alpha , IL 1beta and IFN gamma ) for 24 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The combination of thermal injury plus PAO 1 infection enhanced the expression of several pro inflammatory and haematopoietic cytokines [ stem cell factor ( SCF ) , leukocyte inhibitory factor ( LIF ) , IL 6 and TNF alpha ] ; induced the expression of granulocyte macrophage colony stimulating factor ( GM CSF ) and G CSF by 5 h and the expression of additional cytokines , including TGF beta , TNF beta , lymphotoxin beta ( LT beta ) , interferon gamma ( IFN gamma ) , and IFN beta by 40 h post burn / infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Previous studies of the MLR have shown high levels of type 1 cytokine production , such as IL 1 , IL 6 , IFN gamma and TNF alpha , but low or undetectable levels of type 2 cytokines , such as IL 4 and IL 10 . ^^^ Here , through establishing optimal conditions for the examination of levels and kinetics of a more definitive panel of type 1 / type 2 cytokines ( IL 4 , IL 5 , IL 10 and IL 13 , IFN gamma , TNF alpha and the soluble IL 4 receptor ) we show that , contrary to previously published data , the human alloresponse is truly heterogeneous , resulting in abundant type 2 as well as type 1 cytokine secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The macrophage cytokine IL 1beta in combination with IFN gamma and TNF alpha , plays an important role for beta cell dysfunction and death . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lyso PC inhibited IFN gamma or IFN gamma plus TNF alpha induced IP 10 , Mig , and 1 TAC mRNA expression but not that of the IRF 1 or IRF 1 dependent molecule , GBP . ^^^ It also inhibited IP 10 protein secretion in conditioned media induced by IFN gamma or IFN y plus TNF alpha Western analysis demonstrated that Lyso PC inhibited IFN gamma induced STAT 1alpha protein expression , but not that of IRF 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have characterized the initial response of hepatic endothelium to xenogeneic islets by measuring the expression of Il 1alpha , TNF alpha , IFN gamma , and iNOS in an in vitro dog to pig model of xenoislet transplantation . ^^^ Polymerase chain reaction was done on the cDNA in order to visualize Il 1alpha , TNF alpha , IFN gamma , and iNOS expression . ^^^ Aortic endothelium expressed low levels of IL 1alpha and TNF alpha , but not IFN gamma or iNOS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although tumor necrosis factor alpha and interleukin 1 could be detected in the nasal washes and sera of both strains of mice , the production in serum was transient and much lower than that of IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , CD 40 ligation markedly induced the production of TNF alpha as well as the expression of TNF alpha mRNA in interferon gamma ( IFN gamma ) stimulated BV 2 glial cells . ^^^ Ligation of CD 40 in CD 40 overexpressed cells markedly enhanced the expression of TNF alpha in the presence of IFN gamma . ^^^ Consistently , a dominant negative mutant of p 65 ( Delta ( p 65 ) ) and that of C / EBPbeta ( DeltaC / EBPbeta ) inhibited the expression of TNF alpha in BV 2 microglial cells stimulated with the combination of IFN gamma and CD 40 ligand . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
ELISA assays were used to measure IFN gamma , tumor necrosis factor alpha ( TNF alpha ) , interleukin 2 ( IL 2 ) , IL 4 , IL 6 , and IL 16 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Analysis of liver histopathology , lipid peroxidation , endotoxin , mRNA , and immunohistochemistry for MIF , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) were carried out . ^^^ Male and female rats fed fish oil and ethanol showed necroinflammatory liver injury and had the highest expression of MIF , TNF alpha , and IFN gamma in the liver . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vivo expression of Toll like receptor 2 and 4 by renal epithelial cells : IFN gamma and TNF alpha mediated up regulation during inflammation . ^^^ Using RT PCR , the enhanced TLR 2 and TLR 4 mRNA expression was shown to be completely dependent on the action of IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our results show that compared with CD40L deficient T cells , T cells that express CD40L normally have a minimal advantage in becoming primed , as defined by CD 45 RO isoform expression and production of IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate the effect of colchicine ( the main therapeutic agent for FMF patients ) and certain inflammatory cytokines ( IL 1 beta , TNF alpha , IFN alpha , IFN gamma ) on MEFV expression and C5a inhibitor activity in neutrophils and primary peritoneal fibroblast cultures . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : This study showed a significantly increased production of IFN gamma and IL 1RA and a trend toward a significantly increased production of IL 6 and TNF alpha by whole blood of autistic children . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Experiments with TNF alpha treatment , neutralization of cytokines with antibodies , and analysis of interferon ( IFN ) gamma messenger RNA showed that the mechanism of the EGCg induced recovery of anti L . pneumophila activity impaired by nicotine may be due to the recovery of TNF alpha and IFN gamma production by the macrophages . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We now demonstrate that culture of Ras transformed cells in the presence of the farnesyltransferase inhibitor ( FTI ) LB 42722 leads to up regulation of Fas expression , both under basal growth conditions and in the presence of the inflammatory cytokines IFN gamma and tumor necrosis factor alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , although Th 1 cytokine receptors tumor necrosis factor ( TNF ) RI , interferon ( IFN ) gammaRalpha , IFN gammaRbeta , were detected , their cytokines ( IFN gamma , TNF alpha , lymphotoxin alpha ) were not . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
None of the components at any concentration had any effect on the release of TNF alpha , IFN gamma and IL 2 by the splenocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Levels of the soluble mediators TNF alpha , IFN gamma and IL 1 as well as NO were elevated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It is also known that chronic inflammation can modify the process of erythropoiesis , and this is probably mediated via pro inflammatory cytokines such as interleukin 1 ( IL 1 ) , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Zaprinast also enhanced NO production induced by LPS or IFN gamma ( 100 U / ml ) , and in microglial cell cultures , but not in astrocyte cultures , zaprinast enhanced the basal and the IFN gamma induced production of the cytokines , TNF alpha and IL 1beta , and of NO . ^^^ Zaprinast enhanced the IFN gamma ( 200 U / ml ) induced expression of MHC Class 2 molecules in astrocytes and microglial cells in mixed cultures , but did not enhance this IFN gamma induced expression in pure astrocytes , which lacked paracrine TNF alpha from microglial cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Effect of culture and proinflammatory cytokines ( TNF alpha , IFN gamma ) was studied on phenotype . ^^^ Addition of TNF alpha at 1000 UI / ml to cultures of FIH or IMH increased Class 1 and ICAM 1 expression whereas IFN gamma ( 50 or 1000 UI / ml ) had no evident effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Levels of IL 2 peaked in the first 24 h of culture followed by TNF alpha and IFN gamma levels which peaked at 96 h of culture . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When cytokine production in vitro was examined , a significant defect in PHA induced IL 2 , L 4 and IFN gamma , and in virus induced IFN alpha production , but no significant alterations in LPS induced IL 6 , IL 10 and TNF alpha production were observed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Also , cultured HCE and HCS cells , with IFN gamma ( 250 1000 U / mL ) or TNF alpha ( 500 4000 U / mL ) treatment for 1 to 4 days and with no treatment , were stained by the immunofluorescence technique with mAbs and analyzed by flow cytometry . ^^^ In addition , the expression of CD 40 is induced on cultured HCE and HCS cells differentially by proinflammatory cytokines , such as IFN gamma and TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Knockout versus wild type mice showed a significant decrease in IFN gamma mRNA levels in the cornea and cervical lymph nodes and decreased TNF alpha protein levels in cornea . ^^^ CONCLUSIONS : These data provide evidence that IL 12 is important in IFN gamma production and in the absence of the cytokine , both IFN gamma and TNF alpha levels in cornea are significantly decreased , resulting in unchecked bacterial growth and perforation . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of IP 10 in ESC after stimulation with interferon gamma ( IFN gamma ) , interleukin 1 beta ( IL 1 beta ) , tumour necrosis factor alpha ( TNF alpha ) , or lipopolysaccharide ( LPS ) was evaluated using an enzyme linked immunosorbent assay and Northern blot analysis . ^^^ IFN gamma , IL 1 beta , TNF alpha and LPS significantly stimulated the expression of IP 10 mRNA and protein in ESC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
AIM : To study the effects of aminoguanidine ( AG ) and two L arginine analogues N ( omega ) nitro L arginine methyl ester ( L NAME ) and N ( omega ) nitro L arginine ( L NNA ) on nitric oxide ( NO ) production induced by cytokines ( TNF alpha , IL 1 beta , and IFN gamma ) and bacterial lipopolysaccharide ( LPS ) mixture ( CM ) in the cultured rat hepatocytes , and examine their mechanisms action . ^^^ METHODS : Rat hepatocytes were incubated with AG , L NAME , L NNA , Actinomycin D ( ActD ) and dexamethasone in a medium containing CM ( LPS plus TNF alpha , IL 1 beta , and IFN gamma ) for 24h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The recruitment of ( 111 ) In labeled spleen T cells to intradermal sites injected with IFN gamma , TNF alpha , LPS , poly inosine cytosine , and Con A was measured in the rat , and the effect of blocking mAbs to E selectin , P selectin , very late activation Ag 4 ( VLA 4 ) , and LFA 1 was determined on this T cell migration in vivo . ^^^ Treatment with both anti VLA 4 and anti E selectin mAbs inhibited up to 85 % of the lymphocyte accumulation , while P selectin and VLA 4 blockade in combination was not more effective than VLA 4 blockade alone in TNF alpha , IFN gamma , LPS , and poly inosine cytosine lesions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine production was measured after incubation for 16 20 h using an Elispot method detecting interleukin 10 ( IL 10 ) , IL 6 , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) producing cells . ^^^ In addition , parallel cell culture supernatants were collected and analyzed by ELISA for IL 10 , IL 6 , TNF alpha , IFN gamma , and IL 2 . ^^^ No effects of GSTM were seen on IFN gamma or TNF alpha producing cells . ^^^ Parallel supernatant cultures displayed a GSTM dose dependent decrease in IFN gamma levels after mitogen stimulation , whereas no changes were seen in IL 6 or TNF alpha levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , TNF alpha and IFN gamma levels as well as expression of the activation marker CD 69 were elevated in liver mononuclear cells of WT and WT GTG mice , but not in ob / ob mice following administration of Con A . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Like peripheral NK cells , the CD 161 ( + ) / CD56 ( + ) NK cells from these cultures contained a tumor necrosis factor alpha ( TNF alpha ) ( + ) / granulocyte macrophage colony stimulating factor ( GM CSF ) ( + ) subset , an interferon gamma ( IFN gamma ) ( + ) subset , mostly included within the former , and very few IFN gamma ( ) / IL 13 ( + ) cells . ^^^ Instead , most immature CD 161 ( + ) / CD56 ( ) NK cells , detectable only in the cultures with IL 2 , produced IL 13 , TNF alpha , and GM CSF , but not IFN gamma , and contained an IL 5 ( + ) subset . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we have discovered that a major component of IFN gamma induced CD 40 expression involves the endogenous production of the cytokine TNF alpha . ^^^ The inclusion of anti TNF alpha neutralizing antibody significantly inhibits IFN gamma induced CD 40 mRNA and CD 40 promoter activity . ^^^ IFN gamma induced CD 40 protein expression is attenuated in TNF alpha deficient microglia and can be restored with exogenous TNF alpha . ^^^ IFN gamma treatment leads to the activation of NF kappaB in a time dependent manner , which is inhibited in the presence of anti TNF alpha neutralizing antibody . ^^^ These results indicate that IFN gamma induced TNF alpha production and subsequent NF kappaB activation are integral parts of the mechanism of IFN gamma induced CD 40 expression . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The supernatants were collected for cytokine [ interleukin 4 ( IL 4 ) , IL 6 , IL 10 , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) ] measurement by ELISA . ^^^ Basal IL 4 , IL 6 , IL 10 , and TNF alpha levels were similar for both groups ; however , basal IFN gamma levels were slightly higher in patients with RA compared to controls . ^^^ Con A stimulation significantly increased TNF alpha and IFN gamma levels compared to the basal condition in the control and RA groups , and both cytokines were inhibited only by higher doses of Dex in the RA group . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Viremia increased steadily and reached a plateau of approximately 10 ( 8 ) 10 ( 9 ) copies / ml 4 days before death , while IFN gamma and TNF alpha rose to extremely high levels only shortly before death . ^^^ In contrast , in patient 2 the virus load decreased from 10 ( 7 ) to 10 ( 6 ) copies / ml during the late stage of illness which was paralleled by a decrease in the IFN gamma and TNF alpha levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of NK cells were cell contact dependent , although the secretion of IFN gamma and TNF also contributed to DC maturation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Most functions can be associated with their ability to induce a wide range of chemokines ( MCP 1 , MIP 1alpha , IL 8 ) and cytokines ( e . g . , IL 12 , IFN gamma , TNF alpha , IL 4 , IL 6 , IL 10 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The CD 4 ( + ) CD45RB ( low ) population of cells extracted from the affected large intestine secreted high levels of interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) at the protein and mRNA levels . ^^^ Administration of neutralising antibodies to TNF alpha , but not to IFN gamma , prevented the development of colitis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Primary cultures of orbital fibroblasts and corneal epithelial cells were incubated with TNF alpha , TNF beta , TGF beta 1 , IFN gamma , MIF or blocking anti MIF mABs and extracts of the cells quantitated for DAF , MCP and CD 59 by two site immunoradiometric assays . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma and TNF alpha upregulated ICAM 1 expression on these cells . ^^^ The functional importance of IFN gamma plus TNF a upregulation of ICAM 1 expression on BEAS 2B cells was demonstrated by neutrophil BEAS 2B cell adhesion assays . ^^^ Brief incubation of BEAS 2B cells with IFN gamma plus TNF alpha led initial upregulation of ICAM 1 expression followed by a protracted downregulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We observed enhanced production of IFN gamma and TNF alpha in CD 8 ( + ) , CD 3 ( + ) , and CD 56 ( + ) blood cells , as well as an increase in the number of CCR 5 ( + ) cells in patients suffering from SA compared to those with NP . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pentoxifylline ( PTX ) is commonly used in peripheral blood vessel diseases , however it has also been found to decrease the level of proinflammatory cytokines such as IL 12 , TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Systems studied have included lysis by interleukin 2 ( IL 2 ) activated lymphokine activated killer ( LAK ) cells , tumor necrosis factor alpha ( TNF alpha ) , a p 16 expressing adenovirus vector , suicide gene therapy using the herpes simplex virus tyrosine kinase ( HSV tk ) followed by ganciclovir , and immunomodulatory gene therapy with IL 2 , IL 4 , interferon gamma ( IFN gamma ) , IFN alpha , TNF alpha , granulocyte macrophage colony stimulating factor ( GM CSF ) , IL 6 , and IL 1beta transfected into tumors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After clinical evaluation , the CD 4 cell counts and the cytokine profiles interleukins ( IL ) 2 , 4 , 6 , 10 , 12 , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) were studied in all the subjects at the start and after three months of antituberculosis therapy . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We also find out that certain cytokine serum levels are increased ( IL 2 , TNF alpha , IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , iNOS protein and mRNA expression by HRTEC increased after exposure to a cytokine mixture consisting of interleukin ( IL ) 1beta , tumor necrosis factor alpha ( TNF alpha ) and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Of the investigated proinflammatory cytokines IL 6 , TNF alpha , IL 1beta , and IFN gamma , only IFN gamma up regulated FH specific mRNA up to 6 fold in both primary HC and KC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
HFRPE cells were incubated in the presence of Interferon gamma ( IFN gamma ) , IFN beta , Tumor Necrosis Factor alpha ( TNF alpha ) , Interleukin 1beta ( IL 1beta ) , Tumor Growth Factor beta ( TGF beta ) , and a combination of IFN gamma and TGF beta ( pre incubation and simultaneously incubated ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Chorion cells expressed significant amounts of interleukin ( IL ) 1beta , IL 6 , tumor necrosis factor ( TNF ) alpha , interferon ( IFN ) beta , IFN gamma and granulocyte macrophage colony stimulating factor ( GM CSF ) mRNAs and small amounts of Fas mRNA in response to 4 infection . ^^^ We also examined whether or not TNF alpha , IFN beta , IFN gamma and Fas participated in 4 infection induced apoptotic DNA fragmentation in chorion cells . ^^^ Moreover , TNF alpha , IFN beta , IFN gamma and Fas do not appear to be directly involved in the apoptosis induction of 4 infected chorion cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both LPS and PLC induced high levels of tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 beta ( IL 1 beta 6 , gamma interferon ( IFN gamma ) , MIP 1 alpha MIP 2 in the lungs but did not affect IL 18 levels . ^^^ When inoculated with LPS , IRF 1 gene knockout ( IRF 1 KO ) mice produced lower levels of TNF alpha , IL 1 beta , and IFN gamma than did wild type ( WT ) mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , the recognition of major histocompatibility complex class 1 presented epitopes by antigen specific CD8+ T cells results in the TNF alpha and IFN gamma dependent increase in the activation level of DCs and in the induction of type 1 polarized mature DCs capable of producing high levels of IL 12p70 upon a subsequent CD 40 ligation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , treatment with a combination of IFN gamma , TNF alpha and IL 1beta upregulates inducible nitric oxide synthase , and the resulting markedly enhanced NO production inhibits pancreatic cancer cell growth . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Production of tumor necrosis factor alpha ( TNF alpha ) and other proinflammatory cytokines in RA is largely CD 4 ( + ) T cell dependent and mostly a result of interferon gamma ( IFN gamma ) secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , in comparison to Eta 1+ / + , Eta 1 / CD4+ T cells had reduced expression of IFN gamma and TNF alpha upon ex vivo restimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , IL 1alpha , IL 1beta , TNF alpha , IFN gamma inducible protein 10 , and Th 2 cytokines such as IL 4 and IL 10 did not induce IL 18BPa . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , the production of TNF alpha by macrophages stimulated with IFN gamma and IL 2 is inhibited by IL 4 pretreatment , while responses to both LPS and dsRNA are enhanced . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , pretreatment with monoclonal antibodies against 5 beta 2 TCR , CD80 / CD86 , and CD 154 significantly inhibited TSST 1 induced IL 2 , IFN gamma , and TNF alpha secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The levels of TNF alpha ( P = . 016 ) and IFN gamma ( P = . 012 ) significantly increased with age , suggesting a switch to a more chronic infection phenotype . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In 42 patients undergoing LC ( n = 22 ) and OC ( n = 20 ) Th 1 like and Th 2 like was evaluated before operation and at 6 , 24 and 48 hours after operation for white blood cell counting and cytokines ( IL 4 , IL 6 , IL 10 , IL 13 , IFN gamma , TNF alpha ) in the sera and in the supernatants from circulating mononuclear cells stimulated with phytohemagglutinin or lipopolysaccharide . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytotoxicity of IFN gamma and TNF alpha for vascular endothelial cell is mediated by nitric oxide . ^^^ We found that combined stimulation with interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) induced both cytotoxicity to murine vascular endothelial cell line F 2 and an increase in nitric oxide ( NO ) . ^^^ These results together demonstrated that cytotoxicity of IFN gamma and TNF alpha for endothelial cell F 2 was mediated by NO , suggesting a pathogenic role of cytokine induced NO production in endothelial damage under inflammatory conditions . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Reverse transcription polymerase chain reaction ( RT PCR ) analysis of cytokine expression by T cell lines derived from this population revealed strong expression of interferon gamma ( IFN gamma ) , interferon alpha ( IFN alpha ) , tumour necrosis factor alpha ( TNF alpha ) , tumour necrosis factor beta ( TNF beta ) , interleukin 2 receptor alpha ( IL 2Ralpha ) transcripts , and weak expression of IL 2 and IL 4 . ^^^ RT PCR analysis of cytokine expression by T cell lines which were dominated by gammadelta T cells revealed expression of IFN gamma , TNF alpha , TNF beta , IL 2Ralpha transcripts . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of cultured primary human thyroid cells with IFN gamma and TNF alpha uniquely allows the induction of Fas mediated apoptosis . ^^^ To investigate the role of this cytokine combination in vivo , CBA / J mice were immunized with thyroglobulin and then injected with IFN gamma and TNF alpha . ^^^ Compared with control animals , mice treated with IFN gamma and TNF alpha showed significantly sustained lymphocytic infiltration in the thyroid , which was associated with the destruction of portions of the follicular architecture at wk 6 after initial immunization . ^^^ Furthermore , the number of apoptotic thyroid follicular cells was increased only in the thyroids from mice treated with the IFN gamma and TNF alpha . ^^^ Minimal apoptosis of thyroid epithelial cells was observed unless the mice were pretreated with IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A single oral administration of SAHA to mice dose dependently reduced circulating TNF alpha , IL 1 beta , IL 6 , and IFN gamma induced by lipopolysaccharide ( LPS ) . ^^^ SAHA inhibited nitric oxide release in mouse macrophages stimulated by the combination of TNF alpha plus IFN gamma . ^^^ Human peripheral blood mononuclear cells stimulated with LPS in the presence of SAHA released less TNF alpha , IL 1 beta , IL 12 , and IFN gamma ( 50 % reduction at 100 200 nM ) . ^^^ Steady state mRNA levels for LPS induced TNF alpha and IFN gamma in peripheral blood mononuclear cells were markedly decreased , whereas IL 8 and IL 1 beta mRNA levels were unaffected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In comparison with that observed with untransfected K 562 cells , co culture of LGL with HLA G expressing cells significantly reduced the concentration of all cytokines investigated ( TNF alpha , IL 10 and GM CSF , P < 0 . 01 ; IFN gamma and IL 13 , P < 0 . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The requirement for CD 14 in LPS hyper responsiveness was assessed by comparing CD 14 / and the respective wt mice with respect to their ability to produce TNF and IFN gamma , two recognized indices of LPS activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Cytokine production was increased in cultures from patients with Crohn ' s disease compared to controls ( IFN gamma , p = 0 . 005 ; IL 10 , p = 0 . 003 ; TNF alpha , p = 0 . 03 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
DNA fragmentation and the activation of caspase 3 were determined in HSG cells cultured with TNF alpha or IFN gamma . ^^^ Mitochondrial dysfunction also appeared to be involved in the process because a disruption of mitochondrial transmembrane potential with the activation of caspase 9 was demonstrated in TNF alpha and IFN gamma stimulated HSG cells . ^^^ Activation of caspase 8 was thought to be essential in TNF alpha induced apoptosis of HSG cells ; however , the activation of caspase 8 was not involved in IFN gamma induced apoptosis of HSG cells . ^^^ In contrast , Bcl 2 appeared to be an indispensable regulatory molecule in IFN gamma induced , but not in TNF alpha induced , apoptosis of HSG cells because its expression was inhibited in IFN gamma stimulated , but not in TNF alpha stimulated , cells . ^^^ The inhibitory effect of IFN gamma in Bcl 2 expression was enhanced by coadministration of TNF alpha and , interestingly , apoptosis of HSG cells , as assessed by DNA fragmentation and the activation of caspase 9 and caspase 3 , and disruption of mitochondrial transmembrane potential was also synergistically augmented by TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Evaluation of IFN gamma and TNF alpha as immunological markers of clinical outcome in cutaneous leishmaniasis . ^^^ To evaluate if IFN gamma and TNF alpha levels could be used as markers of therapeutic response in cutaneous leishmaniasis , 54 patients with history of one ulcerated cutaneous lesion , with up to 30 days onset , were enrolled in the study . ^^^ IFN gamma and TNF alpha were measured by ELISA in lymphocyte cultures supernatant before and 60 days after initiating therapy . ^^^ IFN gamma and TNF alpha levels were similar in both groups of patients before therapy . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin 1alpha ( IL 1alpha ) and interferon gamma ( IFN gamma ) but not tumor necrosis factor alpha ( TNF alpha ) also significantly increased nitrotyrosine expression in these cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Flow cytometry was used to assess the differential effects of BFA and MN on surface CD 3 , 4 , 8 , and 69 expression and the intracellular expression of gamma interferon ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) following stimulation with phorbol myristate acetate and ionomycin . ^^^ An analysis of the cytokine concentration in culture supernatants indicated that cells treated with MN released TNF alpha and IFN gamma from the cells , while the BFA treated cells released IFN gamma only . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pentoxifylline ( PTX ) , a phosphodiesterase inhibitor , is known to downregulate tumor necrosis factor alpha ( TNF alpha ) secretion induced by lipopolysacchride ( LPS ) and gamma interferon ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Chemokines are produced in a coordinated and sequential manner , with IL 8 and RANTES induced by TNF alpha during early stages , and MCP 1 , IP 10 , Mig , 1 TAC , 1 309 and MDC induced by IFN gamma during later stages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In order to investigate whether such putative defects were intrinsic to T cells or , rather , secondary to quantitative differences in T cell subset distribution , or to other described disorders , we have used Herpesvirus saimiri ( HVS ) for the targeted transformation of CVID CD4+ and CD8+ T cells and subsequent functional evaluation by flow cytometry of their capacity to generate cell surface ( CD 154 , CD 69 ) or soluble ( IL 2 , TNF alpha , IFN gamma ) help after CD 3 engagement . ^^^ Exactly the same five cell lines showed , in addition , a significantly low induction of IL 2 ( P < or = 0 . 04 ) , but not of TNF alpha or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunocytochemical and flow cytometric analyses had revealed the occurrence of constitutively expressed CD 40 molecules on the surface of long term cultured SGEC lines , which could be further induced by interferon gamma ( IFN gamma ) and IL 1beta cytokines , but not tumour necrosis factor alpha ( TNF alpha ) , IL 4 , IL 6 , granulocyte macrophage colony stimulating factor ( GM CSF ) or IFN alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Plasma was harvested from peripheral blood at different postburn time points for the determination of the levels of LPS , IL 1alpha , IL 6 , TNFalpha , IL 8 , IL 2 , IFN gamma , IL 4 and IL 10 in the rats of the two groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
FB ( 1 ) treatment , suggesting a possible combined role of TNF alpha and IFN gamma in their induction and hepatotoxicity . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
LPS , TNF alpha , or IFN gamma had no effect alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Microglial adhesion to laminin was increased significantly by the proinflammatory cytokines TNF , IFN alpha , and IFN gamma but was decreased by TGF beta 1 , with the TGF beta 1 effect being dominant over the other cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Activated CD8hi and TCR gammadelta+ cells were the primary IFN gamma producers , whereas CD4+ cells constituted an important source of TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The numbers of peripheral blood mononuclear cells ( PBMCs ) with spontaneous expression of IFN gamma was decreased at 72 h and 8 weeks after treatment , whilst no clear effects on TNF alpha , IL 4 , IL 10 , TGF beta expression were observed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha and IFN gamma were not statistically analyzed because only a few patients had detectable serum levels of these cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The aim of our study was to evaluate serum interleukin 6 ( IL 6 ) , interleukin 8 ( IL 8 ) , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) as tumor markers study based on the data about tumor cytokines production and tumor host interactions . ^^^ RESULTS : In the control group the upper limit of normal IL 6 titers ( 95th percentile ) was 5 . 5 pg / ml ; the mean IL 8 concentration was 9 . 6 + / 15 . 1 pg / ml and the upper limit of normal was 37 pg / ml ; serum TNF alpha and IFN gamma were not detectable . ^^^ TNF alpha and IFN gamma levels in ovarian cancer were similar to patients with benign ovarian cysts . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A comparative study on the expressions of IL 4 , IFN gamma and TNF alpha in BMMNC of acute and chronic aplastic anemia patients ] . ^^^ OBJECTIVE : To detect the induced levels of IL 4 , IFN gamma and TNF alpha in the supernatant of bone marrow mononuclear cells ( BMMNC ) and compare the difference of immune status between acute ( SAA ) and chronic ( CAA ) aplastic anemia patients . ^^^ METHODS : Concentrations of IL 4 , IFN gamma and TNF alpha in PHA P induced BMMNC supernatants were determined by ELISA assay in 11 SAA and 13 CAA patients as well as 16 controls . ^^^ RESULTS : ( 1 ) The IFN gamma and TNF alpha levels in AA patients studied were much higher than that in controls , and IL 4 levels were normal in SAA group but elevated in CAA group . ( 2 ) TNF alpha levels were comparable between the two AA groups , but both IL 4 and IFN gamma levels were significantly different between them . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : It is shown that interferon gamma ( IFN gamma ) , interleukin 1 ( IL 1 ) and tumor necrosis factor ( TNF ) could induce the expression of orbital fibroblast HLA 2 antigens . ^^^ CONCLUSION : HLA 2 antigen expression can be induced by IFN gamma , IL 1 and TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The development of this inflammatory process is dependent upon the induction of a robust Th 1 response , including overproduction of IFN gamma , TNF alpha , and NO , as has been reported in other experimental models of human inflammatory bowel disease . ^^^ Primed LP CD 4 ( + ) T cells isolated from parasite infected mice produce substantial quantities of both IFN gamma and TNF alpha . ^^^ IFN gamma and TNF alpha producing LP CD 4 ( + ) T cells synergize with infected mIC ( cl 2 ) and enhance the production of several inflammatory chemokines including macrophage inflammatory protein 2 , monocyte chemoattractant protein 1 , monocyte chemoattractant protein 3 , macrophage inflammatory protein 1alphabeta , and IFN gamma inducible protein 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Real time quantitative ( TaqMan ) RT PCR determined mRNA expression of IL 8 , TNF alpha , and the Th 1 markers IFN gamma and the IL 12R beta 2 chain . ^^^ The mRNA expression of TNF alpha , IFN gamma , and IL 12R beta 2 was higher in H . pylori positive patients than in controls , but it did not differ significantly between patients infected with different strain types . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The size of this 10 specific T cell response was comparable to that of the T cell response induced following an acute LCMV infection . ( 2 ) 10 specific CD 8 and CD 4 T cells were capable of producing gamma interferon ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and interleukin 2 ; all cells were able to make IFN gamma , a subset produced both IFN gamma and TNF alpha , and another subset produced all three cytokines . ( 3 ) The CD 8 T cell response to the foreign gene ( LCMV NP 118 126 epitope ) was coordinately regulated with the response to the vector during all three phases ( expansion , contraction , and memory ) of the T cell response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , T cells of children exposed to Aspergillus showed a significantly lower content of TH 1 cytokines ( IFN gamma , TNF alpha , IL 2 ) producing cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Heavy metals like CdCl 2 can induce , or inhibit the synthesis and expression of the inflammatory cytokines IL 1beta , IL 4 , IL 6 , TNF alpha , IFN gamma and ICAM 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Unlike IL 2 , IL 4 , IL 5 , IL 10 , IL 12 and IFN gamma , significant differences were observed in serum IL 6 , IL 18 , TNFalpha and TNFbeta between groups : both IL 6 and IL 18 were higher in postmenopausal women , while TNFalpha and TNFbeta were significantly lower . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of the 1 34 N terminal parathyroid hormone related protein fragment ( 0 10 microM ) and the 67 86 parathyroid hormone related protein fragment ( 0 10 microM ) on trophoblast apoptosis , induced by TNFalpha and IFN gamma or by the protein kinase inhibitor staurosporine , were investigated . ^^^ TNFalpha / IFN gamma and staurosporine significantly increased the rate of apoptosis by fivefold and by eightfold , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Liposome entrapped glycoprotein antigens / allergens of A . fumigatus elicited a Th 1 type response with increased levels of TNF alpha ( 5 . 5 folds ) , IFN gamma ( four folds ) , specific IgG ( three folds ) and IgG2a ( 2 . 4 folds ) , low titers of specific IgG 1 ( 2 . 2 folds decrease ) and IgE ( three folds decrease ) , and decreased peripheral eosinophilia by four folds in comparison to mice receiving free glycoprotein allergens / antigens of A . fumigatus . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Liposome stimulation resulted in the release of IL 6 , IL 10 , IL 1beta , TNF alpha and IFN gamma . ^^^ This indicates that signal transduction via PKC or PTK is necessary , in order for human peripheral blood cells to release cytokines ( IL 6 , IL 10 , IL 1beta , TNF alpha and IFN gamma ) as the result of liposome stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Natural killer ( NK ) cells , total , B , T , T 4 , T 8 lymphocytes , proliferation of lymphocytes , in vitro production of IL 2 , IFN gamma , TNF alpha , and IL 2R expression were measured . ^^^ Production of interleukin 2 ( IL 2 ) , interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) was augmented , and IL 2R expression less enhanced compared with the controls . ^^^ It is concluded that administration of 0 . 2 g / kg per day fish oil after a moderate surgical stress is not immunosuppressive , but enhances the production of IFN gamma , TNF alpha and possibly IL 2 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To understand the mechanism ( s ) implicated in the regulation of the synthesis and release of IL 10 during early infection , we investigated the autocrine effects of IL 6 , IL 12 , tumor necrosis factor alpha ( TNF alpha ) , and IL 10 itself , as well as the exocrine effect of IFN gamma on the production of macrophage derived IL 10 with lipoprotein as a stimulant . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our in vitro data showed that intestinal epithelial cells infected by C . parvum or stimulated by the proinflammatory cytokines ( IFN gamma , interleukin 1 beta , and tumor necrosis factor alpha ) produce a pattern of chemokine secretion similar to that observed in vivo , suggesting that these cells may take part in the initial production of chemokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When M . tuberculosis infected mice were treated with recombinant BCG that secreted cytokines shown to reduce disease in a preinfection vaccine model , the BCG secreting TNF alpha , and to a lesser extent , IL 2 and gamma interferon ( IFN gamma ) , caused a significant increase in granuloma size in the lungs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
BACKGROUND : Inflammatory mediators such as tumor necrosis factor alpha ( TNF alpha ) , interleukin 1beta ( IL 1beta ) , IL 6 , and interferon gamma ( IFN gamma ) may change coronary plaque integrity by altering vascular smooth muscle cell ( VSMC ) survival and modifying the extracellular matrix . ^^^ METHODS AND RESULTS : To determine the effects of cytokines on the IGF 1 system , rat aortic VSMCs were exposed to TNF alpha ( 10 to 500 ng / mL ) , IL 1beta ( 20 pg to 10 ng / mL ) , IL 6 ( 100 pg to 15 ng / mL ) , or IFN gamma ( 10 to 600 U / mL ) . ^^^ In addition , apoptosis induced by TNF alpha , IFN gamma , and Fas ligand was markedly reduced by desamino ( 1 3 ) IGF 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OVA CTL produced type 1 cytokines IFN gamma and TNF alpha , whereas INS CTL produced IL 5 and IL 10 with low levels of IL 4 and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human recombinant interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , or both ( cytomix ) were added to a final concentration of 10 ng / ml . ^^^ The average increase induced with IFN gamma , TNF alpha , and cytomix was 1 . 8 , 2 . 33 , and 2 . 31 fold . ^^^ We also demonstrated that nasal epithelial cells have the potential to express iNOS protein spontaneously or upon stimulation with inflammatory cytokines , such as IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study was performed to determine the distribution , in African American and Caucasian renal transplant recipients , of single nucleotide polymorphisms ( SNPs ) in the following cytokine genes : tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , interleukin ( IL ) 6 , IL 10 , and transforming growth factor beta ( TGF beta ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
By contrast , the mean fluorescence intensity of staining for the CD 44 glycoprotein was diminished on CD 8 ( + ) D ( b ) p 56 ( + ) T cells from the 1 A ( b / ) group , the level of CTL activity was lower on a per cell basis , and the relative prevalence of IFN gamma ( + ) TNF alpha ( + ) T cells detected after in vitro stimulation with the p 56 peptide was decreased . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These CTL mediated tumor specific H 2K ( d ) restricted lysis and production of IFN gamma , TNF alpha , and Fas ligand ( FasL ) in response to Renca . ^^^ The cytokines IFN gamma and TNF alpha increased the sensitivity of Renca cells to CTL lysis by both granule and FasL mediated death pathways . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cells were cultured for up to 3 days in the presence of 2000 pg / ml of human IL 1beta , 1000 U / ml of TNF alpha , and 1000 U / ml of IFN gamma , as well as 1000 U / ml of porcine IFN gamma . ^^^ Adult pig islets are sensitive to the toxic effects of human TNF alpha , IL 1beta , the combination of the three cytokines , and porcine IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We report the relationship between priming of CD 4 and CD 8 T cells for interleukin 2 ( IL 2 ) , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) and disease evolution in MS patients , clinically subdivided into relapsing remitting MS ( RRMS ) in remission , RRMS in relapse , or chronic progressive MS ( CPMS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The aim of our study was to evaluate the frequency of C . pneumoniae infection in abdominal aortic aneurysm ( AAA ) patients by measuring C . pneumoniae specific serum IgG , IgM and IgA levels and the activation of their immune system by measuring the concentrations of IL 10 , IL 12 , IFN gamma and TNF alpha in patients ' serum . ^^^ Mean concentrations of IL 10 , IL 12 , IFN gamma and TNF alpha indicated lack of protection against intracellular pathogens . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study examines immediate nitric oxide ( NO ) release from monocytes following interleukin 1beta ( IL 1beta ) , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) challenge in patients with complex regional pain syndrome ( CRPS ) . ^^^ Peripheral blood monocytes were challenged with 100 microl of IL 1beta ( 1 ng ) , IFN gamma ( 1 ng ) , TNF alpha ( 0 . 01 ng ) , and normal saline ( NS ) and the resultant immediate NO release measured . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
HAS 3 mRNA expression was increased by a maximum of 4 . 3 times after 3 hour stimulation with IL 1beta ( 10 ng / ml ) , whereas 1 hour stimulation with TNF alpha ( 10 ng / ml ) and IFN gamma ( 10 ng / ml ) induced around a 2 . 5 fold increase in HAS 3 mRNA . ^^^ Although IFN gamma ( 1 100 ng / ml ) alone showed little effect on HAS 2 mRNA expression , the effect was synergized by combined with both IL 1beta and TNF alpha , substantially increasing HAS 2 mRNA expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma / TNF alpha synergism in MHC class 2 induction : effect of nicotinamide on MHC class 2 expression but not on islet cell apoptosis . ^^^ AIMS / HYPOTHESIS : Interferon gamma ( IFN gamma ) and TNFalpha synergistically induce pancreatic beta cell apoptosis . ^^^ TNFalpha alone did not induce MHC class 2 expression , but enhanced IFN gamma induced MHC class 2 expression . ^^^ Nicotinamide reduced MHC class 2 expression by cytokine ( s ) but did not protect insulinoma cell apoptosis by IFN gamma and TNFalpha in combination or protect against the development of diabetes mellitus after adoptive transfer of diabetogenic lymphocytes . ^^^ CONCLUSION / INTERPRETATION : IFN gamma and TNFalpha synergistically induced MHC class 2 expression on insulinoma cells through the induction of CIITA ; nicotinamide reduced the expression of cytokine induced MHC class 2 expression on insulinoma cells through its effect on CIITA expression ; and the preventive effect of nicotimamide on Type 1 ( insulin dependent ) diabetes mellitus is probably due to its effect of MHC class 2 expression rather than that on islet cell apoptosis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vivo plasma cytokine ( IFN gamma , TNF alpha , IL 6 , and IL 12 ) levels and in vitro cytokine production by hepatic mononuclear cells were significantly higher in B 6 mice compared with GKO mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
DESIGN AND METHODS : We analysed the effect of the human recombinant proinflammatory cytokines interleukin ( IL ) 6 and IL 1beta , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) on human type 1 5 ' iodothyronine deiodinase ( 5 ' DI ) enzyme activity in the human hepatocarcinoma cell line HepG 2 , i . e . in a homologous human system . ^^^ RESULTS : IL 6 at 500 pg / ml and TNF alpha at 25 ng / ml had no significant effect , whereas 100 ng / ml IFN gamma or 10 ng / ml IL 1beta reduced 5 ' DI enzyme activity to 77 . 9 and 59 . 5 % of control values . ^^^ IFN gamma did not alter , IL 6 and TNF alpha moderately decreased ( in the case of IL 6 only in the CAT system ) , and IL 1beta ( 0 . 01 10 ng / ml ) dose dependently inhibited 5 ' DI promoter activity to a minimum of 38 . 1 % . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Distribution of IFN gamma , IL 4 and TNF alpha protein and CD 8 T cells producing IL 12p40 mRNA in human lung tuberculous granulomas . ^^^ Paraffin embedded tissue from the lungs of seven patients with pulmonary tuberculosis was analysed by immunohistochemistry and in situ hybridization to detect interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) and interleukin 4 ( IL 4 ) proteins and IL 12p40 mRNA . ^^^ All seven patients had granulomas staining positive for IFN gamma , TNF alpha and IL 12p40 , but only four stained positive for IL 4 . ^^^ Cells with the morphology of lymphocytes , macrophages and giant cells expressed TNF alpha , IFN gamma and IL 4 protein . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Regulated on activation , normal T cells expressed and secreted ( RANTES ) was also induced by TNF alpha and was drastically increased by the addition of IFN gamma . ^^^ Eosinophil chemoattractant activity in culture supernatants of NPMC stimulated with TNF alpha plus IL 4 was diminished by an anti eotaxin antibody ; that induced by TNF alpha plus IFN gamma was attenuated by an anti RANTES antibody . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neutralization of TNF by TNF binding proteins resulted in only a 36 % reduction of IFN gamma synthesis . ^^^ Thus , C . albicans stimulates IFN gamma production in an IL 18 , IL 12 , and IL 1beta dependent manner , whereas production of TNF and IL 8 is independent of these cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma regulates MD 2 expression in both IEC lines , whereas IFN gamma and TNF alpha regulate TLR 4 mRNA expression in IEC lines . ^^^ Pre incubation with IFN gamma and / or TNF alpha sensitizes IEC to LPS dependent interleukin 8 secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The markedly increased concentrations of TNF alpha and IFN gamma in the peripheral blood at the time of HPS declined gradually , and the IL 6 concentration increased at the time of diagnosis of Still ' s disease . ^^^ As HPS and Still ' s disease have a common etiology , and each shows high concentrations of IFN gamma , IL 6 and TNF alpha , the symptoms are similar in both diseases . ^^^ In particular , a relationship between HPS and high concentrations of TNF alpha and IFN gamma is suspected . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor ( TNF ) activity was assayed by measuring its cytotoxic activity on L 929 cells , and interleukin 6 ( IL 6 ) and interferon gamma ( IFN gamma ) were assayed by enzyme linked immunosorbent assay , whereas NO levels were detected by Griess colorimetric reactions in culture supernatant of macrophages incubated with TSV and subsequently exposed to either lipopolysaccharide or IFN gamma . ^^^ Tumor necrosis factor ( TNF ) activity was assayed by measuring its cytotoxic activity on L 929 cells , and interleukin 6 ( IL 6 ) and interferon gamma ( IFN gamma ) were assayed by enzyme linked immunosorbent assay , whereas NO levels were detected by Griess colorimetric reactions in culture supernatant of macrophages incubated with TSV and subsequently exposed to either lipopolysaccharide or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine ( TNFalpha , IL 1alpha , IL 1beta , IL 2R , IL 6 , IL 12 and IFN gamma ) fingerprinting of the PLNs after injection of ethanol and imipramine evidenced the same pattern of responses . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Reduced numbers of interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) producing T cells were found in association with biparental FAH and housing renovation during pregnancy . ^^^ Neonates from Munich showed significantly decreased amounts of IFN gamma and TNF alpha and elevated levels of interleukin 4 ( IL 4 ) producing T cells . ^^^ Differences in cytokine production between Munich and Leipzig were influenced by season ( IL 4 ) and housing renovation ( IFN gamma , TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of N ( G ) monomethyl L arginine ( NMMA ) , polymyxin B and cytokines ( IFN gamma , TNF alpha , IL 4 and IL 12 ) on the production of NO were also determined . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Priming effects of GM CSF , IFN gamma and TNF alpha on human neutrophil inflammatory cytokine production . ^^^ In the present study we examined the role of granulocyte / macrophage colony stimulating factor ( GM CSF ) , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) as priming agents for interleukin ( IL ) 1beta , IL 6 and TNF alpha production by stimulated neutrophils from control subjects and malignant melanoma patients . ^^^ The ability of GM CSF , IFN gamma and TNF alpha to serve as effective priming agents for inflammatory mediator production by PMNs revealed a new role for these cytokines in the innate immune response of the melanoma bearing host . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Normal concentrations of IL 1beta , IL 6 , IFN gamma and TNF alpha were present in CSF and serum in almost all patients with lumbar disc herniation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The water extract of perilla leaves induced nitric oxide ( NO ) production of VSMC and this effect was synergistically augmented when combined with interferon ( IFN ) gamma or tumour necrosis factor ( TNF ) alpha , while the perilla extract significantly inhibited NO production induced by IFN gamma combined with lipopolysaccharide ( LPS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of mice with anti CD 154 reduced characteristic immunological parameters in type 2 responses ( B and CD 4 ( + ) T cell proliferation , IgG 1 and IgE Abs , and IL 4 secretion ) and only slightly affected the type 1 response ( small decrease in IFN gamma production , influx of CD11c ( + ) and F4 / 80 ( + ) cells , and prevention of architectural disruption of the lymph node , but no effect on IgG2a Ab and TNF alpha secretion or B and CD 4 ( + ) T cell proliferation ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Thus , spleen cells from infected transgenic mice produced higher levels of TNF alpha and IFN gamma and less IL 5 and IL 10 upon stimulation ex vivo with the exotoxin toxic shock syndrome toxin 1 compared with congenic controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The serum concentrations of tumor necrosis factor beta ( TNF beta ) , TNF receptor 1 ( TNFR 1 ; 55 kDa ) and TNFR 2 ( 75 kDa ) , interleukin 4 ( IL 4 ) , interleukin 10 ( IL 10 ) and interferon gamma ( IFN gamma ) were measured by enzyme linked immunosorbent assay in all patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Evaluated parameters were the levels of the following cytokines : IL 1beta , IL 4 , IL 6 , IL 8 , IL 12 , IFN gamma and TNF alpha . ^^^ RESULTS : Allergic subjects showed a significant increase of IL 4 ( P < 0 . 01 ) and TNF alpha ( P < 0 . 05 ) and a significant decrease of IL 12 ( P < 0 . 05 ) and of IFN gamma ( P < 0 . 0001 ) , whereas IL 1beta , IL 6 and IL 8 were not significantly increased . ^^^ Significant correlations were demonstrated between IL 4 and IL 12 ( P < 0 . 001 ) , IL 12 and IFN gamma ( P < 0 . 001 ) , IL 8 and neutrophils ( P < 0 . 01 ) , and TNF alpha and monocytes / macrophages ( P < 0 . 05 ) , in allergic asthmatics . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In whole lumbar cord tissue , both the nerve growth factors and the proinflammatory cytokines , IFN gamma and TNF alpha , displayed similar expression patterns , peaking at the height of the disease . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytotoxicity of 904 T 1 was not mediated by a T cell antigen receptor or by Fas ligand , but by perforin based cytolytic pathways , and was enhanced by interleukin ( IL ) 12 . 904 T 1 cells produced large amounts of interferon ( IFN ) gamma , but not tumor necrosis factor ( TNF ) alpha or IL 4 in response to autologous tumor cells , and produced high levels of IFN gamma and TNF alpha , and a substantial level of IL 4 following stimulation with anti CD 3 monoclonal antibody . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inflammatory cytokines such as tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) can exert detrimental effects in the placenta and tend to be present at low concentrations , whereas the regulatory cytokines interleukin ( IL ) 10 and tranforming growth factor beta ( TGF beta ) are beneficial and tend to predominate . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Incubation of IFN gamma primed MonoMac 6 cells with serum opsonized zymosan or EGP 2 directed , mouse IgG2a opsonized , EGP 2 positive tumor cells resulted in the production of ROS and TNF alpha and induced E selectin and ICAM 1 expression on HUVECs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , numerous cytokines were detected in CNS 1 tumors in vivo including Ltbeta , IL 1alpha , IL 1beta , TNF alpha , TNF beta , IL 10 , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of IL 1alpha , IL 1beta , IL 6 , IL 10 , IL 12 , IFN beta , IFN gamma , LT beta , TGF beta , TNF a and TNF beta mRNA levels in CRT MG human astroglioma cells upon Fas ligation was investigated using RNase protection assay ( RPA ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CMV stimulation induced tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) in both CD 4 ( + ) and CD 8 ( + ) cells ( usually together ) , with a shift from memory to effector cell phenotype , while only a small proportion of CD 4 ( + ) cells produced IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunological parameters were evaluated by interleukin ( IL 2 , IL 4 , IL 6 , IL 8 , IL 10 , TNF alpha , IFN gamma ) and levels of lymphocyte subsets ( T cell , B cell , T 4 , T 8 and T4 / T8 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 6 , 8 , 12 and TNF alpha transcripts were found in 94 % , 94 % , 78 % and 56 % of distemper dogs , respectively , compared to 17 % , 33 % , 0 % and 0 % in controls , whereas IL 1beta , 2 and IFN gamma were not detectable in any of the studied cerebella . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Resiquimod stimulates specific cells of the innate immune system ( including monocytes / macrophages , dendritic cells ( DC ) and B lymphocytes ) to produce cytokines ( in particular IFN alpha , IL 12 , TNF alpha and IFN gamma ) that initiate and drive the development of the Th 1 acquired immune response against HSV infected cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 5 , IL 6 , TNF alpha , IFN gamma ) and chemokines ( i . e . regulated on activation , normal T cell expressed and secreted ; eotaxin ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF and IFN gamma protein production was measured using a bioassay and an ELISA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In normal human keratinocytes , proinflammatory cytokines either independently ( IL 1alpha ) or synergistically ( tumour necrosis factor alpha ( TNF alpha ) / interferon gamma ( IFN gamma ) and TNF alpha / IL 4 ) up regulated eotaxin expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
THI was characterized by an increased frequency of CD3+ / CD4+ lymphocytes expressing tumour necrosis factor alpha ( TNF alpha ) , TNF beta and interleukin 10 ( IL 10 ) , while in SIgAD elevated numbers of these cells containing TNF alpha and interferon gamma ( IFN gamma ) were observed . ^^^ In low producers of IgG an increased proportion of CD3+ / CD4+ cells expressing TNF alpha and IFN gamma was found , while in low IgA responders only elevated TNF alpha positive CD3+ / CD4+ cells were observed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
INTRODUCTION : Apoptosis can be induced in cytotrophoblasts and syncytiotrophoblast in culture by a combination of TNF alpha and IFN gamma ( Yui et al . , 1994a ; Garcia Lloret et al . , 1996 ) . ^^^ This apoptotic action of TNF alpha and IFN gamma can be inhibited / ' rescued ' by EGF ( Garcia Lloret et al . , 1996 ) . ^^^ Additional ' survival ' factors have been sought which might protect cells against apoptosis induced by TNF alpha / IFN gamma . ^^^ Apoptosis was induced using TNF alpha / IFN gamma , and rescue was attempted using the various cytokines . ^^^ RESULTS : As in previous studies EGF produced a complete inhibition of the apoptotic action of TNF alpha / IFN gamma . bFGF , IGF 1 , and PDGF AA produced a partial but significant inhibition of the apoptotic action of TNF alpha / IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mucosal tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) were determined by immunoassay . ^^^ Colonic mRNA expression for iNOS , TNF alpha , and IFN gamma was measured by reverse transcription polymerase chain reaction , and NF kappaB activation was evaluated by electrophoretic mobility shift assay . ^^^ After DSS administration , mice showed increased luminal nitrite / nitrate , mucosal TNF alpha and IFN gamma , and mRNA for iNOS and these cytokines , in addition to decreased colonic length and increased inflammatory score , luminal hemoglobin , and colonic myeloperoxidase activity . ^^^ PBN inhibited increases in luminal nitric oxide production , nitrotyrosine immunoreactivity , and mucosal TNF alpha and IFN gamma . ^^^ Colonic iNOS , TNF alpha , and IFN gamma mRNA were suppressed by PBN , as was a DSS induced increase in colonic NF kappaB DNA binding activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To test this hypothesis , effects of IFN gamma or TNF alpha on expression of intercellular adhesion molecule ( ICAM ) 1 , rhinovirus binding , and virus induced chemokine secretion on A 549 and human bronchial epithelial cells ( HBEC ) were determined . ^^^ IFN gamma was a stronger inducer of ICAM 1 and viral binding on HBEC , whereas TNF alpha had greater effects on A 549 cells . ^^^ In addition , IFN gamma , but not TNF alpha , synergistically enhanced regulated on activation , normal T cells expressed and secreted ( RANTES ) mRNA expression and protein secretion induced by RV 16 or RV 49 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This survival does not appear to be dependent on the cytokines TNF alpha , IL 1 beta , IL 6 , and IFN gamma , nor is it dependent on the transcription factor NF kappa B . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The increase in bacterial load in MK 2 ( / ) mice was associated with normal levels of IL 1 beta , IL 6 , and IFN gamma , whereas TNF production was strongly attenuated . ^^^ These results indicate an essential role of MK 2 in host defense against intracellular bacteria probably via regulation of TNF and IFN gamma production required for activation of antibacterial effector mechanisms . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Infection induced an increase in mRNA expression of IL 18 , IL 12p40 , IFN gamma , and TNF alpha in the case of P . yoelii 265 and an increase of IL 18 , IL 12p40 , and IFN gamma in the case of P . berghei ANKA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Splenocyte stimulation by T . gondii antigen produced lymphoproliferative response and cytokine profile ( IL 10 , IL 12 , IFN gamma and TNF alpha ) similar to those produced by chronic natural infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In patients with high erythrocyte sedimentation rate ( > 25 mm / h ) , TNF alpha ( > 6 . 5 pg / ml ) or IL 12 ( > 4 . 7 pg / ml ) serum levels or detectable IFN gamma concentrations hyporesponsiveness to gammac chain cytokines was even more pronounced in SLE lymphoblasts , but not in lymphoblasts from the other groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our data are compatible with the notion that interferon gamma ( IFN gamma ) / tumor necrosis factor ( TNF ) / nitric oxide ( NO ) induced apoptosis of beta cells in experimental diabetes is attenuated by PTX . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
At concentrations of 10 ( 6 ) to 10 ( 5 ) M , NE inhibited TNF alpha , IFN gamma , and GM CSF secretion by NK cells , which are essential for IL 2 driven NK maturation and functions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RA synovial cells were stimulated for 24 h with IL 1 beta ( 1 ng / ml ) , TNF alpha ( 500 pg / ml ) , IFN gamma ( 10 ( 4 ) IU / ml ) alone or in combination . ^^^ The addition of IL 1 beta , TNF alpha , and IFN gamma together increased nitrite production : 257 . 5 + / 35 . 8 % and S nitrosothiol production : 413 + / 29 % , P < 0 . 001 . ^^^ IL 4 , IL 10 , IL 13 , and TGF beta strongly decreased the NO production caused by the combination of IL 1 beta , TNF alpha , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Administration of anti TNF alpha monoclonal antibody ( day 0 ) diminished the lung levels of TNF alpha , interleukin 12 ( IL 12 ) , and gamma interferon ( IFN gamma ) induced by C . neoformans at day 7 postinfection . ^^^ Neutralization of TNF alpha ( day 0 ) also altered the IFN gamma / IL 4 ratio in the lung associated lymph nodes at day 7 following C . neoformans infection . ^^^ These findings demonstrate that afferent phase TNF alpha production is essential for the induction of IL 12 and IFN gamma and neutralization of early TNF alpha results in a T 2 shift of the T1 / T2 balance of antifungal immunity . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma , IL 1beta , and TNF alpha levels in the gut and skin correlated with the extent of inflammation in both organs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the first series of experiments IDO induction was investigated by direct application of the cytokines IFN gamma , TNF alpha , IL 12 and IL 18 in different concentrations . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , 10 ( 4 M NaVO 3 significantly reduced the release of IFN gamma by PHA stimulated PBMCs , and 10 ( 7 ) M NaVO 3 significantly enhanced the release of TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OBJECTIVE : To evaluate cytokine production ( interferon gamma [ IFN gamma ] , interleukin 1 [ IL 1 ] , interleukin 2 [ IL 2 ] , interleukin 6 [ IL 6 ] , interleukin 10 [ IL 10 ] , interleukin 4 [ IL 4 ] and tumour necrosis factor alpha [ TNF alpha ] ) in patients with tuberculous pericarditis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To that end , we targeted our investigation to five candidate gene loci transforming growth factor beta 1 ( TGF beta 1 ) , interferon gamma ( IFN gamma ) , epidermal growth factor ( EGF ) , interleukin 1 beta ( IL 1beta ) and tumour necrosis factor ( TNF alpha ) genes . ^^^ We investigated the distribution of genotypes and alleles of the six following dimorphic variants : TGF beta 1 ( * ) 10 ( T > C ) and TGF beta 1 ( * ) 25 ( G > C ) , located at codons 10 and 25 , respectively , of TGF beta 1 ; T874A in intron 1 of IFN gamma ; G61A in exon 1 of EGF ; TaqI dimorphism at +3962 ( exon 5 ) of IL 1beta ; and 308A > G in the promoter of TNF alpha . ^^^ These six bi allelic markers were visualised by methods based on the techniques of amplification refractory mutation system polymerase chain reaction ( for TGF beta 1 , IFN gamma , EGF and TNF alpha ) and by polymerase chain reaction TaqI restriction endonuclease analysis in the case of IL 1beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CLA decreased the interferon gamma ( IFN gamma ) induced mRNA expression of mediators of inflammation including cyclooxygenase 2 ( COX 2 ) , inducible NOS ( iNOS ) , and tumor necrosis factor alpha ( TNFalpha ) . ^^^ Consequently , CLA decreased the production of PGE ( 2 ) , TNFalpha and the inflammatory agent nitric oxide ( NO ) in RAW cells treated with IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
First trimester human trophoblast primary culture cells as well as A 3 and HTR / 8 trophoblast cell lines were treated with proinflammatory cytokines such as interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNFalpha ) and with the anti inflammatory cytokines interleukin ( IL ) 6 and IL 10 . ^^^ IFN gamma and TNFalpha promote Fas expression and sensitivity , whereas IL 6 and IL 10 increase the resistance of trophoblast cells to Fas mediated apoptosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine secretion ( TNF alpha , IFN gamma , IL 2 , IL 4 , IL 5 , and IL 10 ) was measured in the supernatants of the cultures stimulated with 21 overlapping preproinsulin peptides as well as proinsulin and insulin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To examine the direct effects of LSF on beta cells , insulin secreting INS 1 cells were exposed to a combination of recombinant IL 1 beta , TNF alpha , and IFN gamma with or without LSF for 18 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Isolated ECL cells were found to express the TNF receptor p 55 and IFN gamma receptor , but not the TNF receptor p 75 or CD 95 . ^^^ TNF alpha ( 25 ng / ml ) increased apoptosis in ECL cells approximately 4 fold , IFN gamma had no effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
YH Tang significantly inhibited interleukin ( IL ) 1 , IL 4 , IL 6 and tumor necrosis factor alpha ( TNF alpha ) secretion in astrocytes stimulated with SP and LPS , but did not inhibit interferon y ( IFN gamma ) and IL 2 secretion significantly . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Because disease severity was increased in IFN gamma ( / ) vs IL 18 neutralized mice , and since IL 18 also induces production of TNF , we tested for TNF alpha in both groups . ^^^ ELISA analysis demonstrated significantly elevated corneal TNF alpha protein levels in IFN gamma ( / ) vs wt mice after infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Mature , confluent enterocytes were pretreated 48 to 72 h with tumor necrosis factor alpha ( TNF alpha ) , interferon gamma , ( IFN gamma ) , or interleukin 4 ( IL 4 ) . ^^^ RESULTS : TNF alpha , IFN gamma , and IL 4 had variable effects on bacterial adherence to HT 29 and Caco 2 enterocytes , although the most consistent finding was increased bacterial adherence associated with INF gamma . ^^^ Bacterial transmigration across confluent HT 29 enterocytes was not altered by TNF alpha , IFN gamma , or IL 4 ; however , IL 4 consistently decreased bacterial transmigration across confluent Caco 2 enterocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human CRP has three effects on cultured encephalitogenic cells that could contribute to the protective effect observed in vivo : 1 ) CRP inhibits encephalitogenic peptide induced proliferation of T cells ; 2 ) CRP inhibits production of inflammatory cytokines ( TNF alpha , IFN gamma ) and chemokines ( macrophage inflammatory protein 1alpha , RANTES , monocyte chemoattractant protein 1 ) ; and 3 ) CRP increases IL 10 production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The healthy individuals exhibited Th 2 , Th 0 , and T regulatory cell 1 like cytokine profiles , whereas TCC from one sibling with MS had a striking Th 1 like phenotype , producing high levels of IFN gamma and TNF alpha , and low IL 4 levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Over a 12 month period , a longitudinal analysis of G CSF , TNF alpha and IFN gamma serum concentrations , burst activity of PMN , and expression of CD 64 on the surface of PMN , were performed by ELISA technique and flow cytometric analysis , respectively , in 58 patients admitted to the intensive care unit ( ICU ) on a daily basis until discharge from the ICU or death . ^^^ TNF alpha serum concentrations were elevated in most episodes ( 22 / 26 ) , whereas IFN gamma serum concentrations were below the detection level in 23 / 26 episodes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The lymph node cells of rats with PIA suppressed by CFA showed significantly increased mRNA expression of IFN gamma but no significant changes in mRNA expression of IL 2 , TNF alpha , IL 4 , and IL 5 compared with those of rats in the control groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In SK Hep 1 cells , GP 73 expression was increased in response to interferon gamma ( IFN gamma ) , and inhibited by tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CONCLUSION : Our investigations in some cases clearly demonstrate that the combination of a second cytokine with TNF alpha , IL 1 beta or IFN gamma can either synergistically or antagonistically modulate the expression of adhesion molecules on HUVECs . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 6 , IL 8 , tumor necrosis factor ( TNF ) alpha , IL 12 and IL 1 beta were induced upon CD 40 ligation and IFN gamma stimulation , while IL 10 could be induced by CD 40 ligation alone and was reduced again by the addition of IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Antibodies and cellular response against chaperonins : HSP 60 and their analogues from bacterial pathogens such as HSP 65 , GroEi etc . ) as well as release of cytokines , adhesion molecules and inflammatory mediators ( CD 40 / CD 40 L , IL 15 , IFN gamma , IL 1 beta , TNF alpha ) also take part in the process . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pretreatment of the cells with LY 294002 resulted in the inhibition of TNF alpha and IL 6 production in RAW264 . 7 cells stimulated with IFN gamma plus LPS or IFN gamma plus PMA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Total RNA was extracted from lung tissue and reverse transcriptase polymerase chain reaction ( RT PCR ) was performed using primers for IL 2 , IL 4 , IL 5 , IL 6 , IL 10 , TNF alpha , IFN gamma , iNOS and beta actin . ^^^ Group 2 had higher IL 2 , IL 4 , IL 10 and IFN gamma cytokine levels in BAL fluid and higher IL 2 , IL 4 , IL 5 , IL 6 , IL 10 , IFN gamma , TNF alpha and iNOS mRNA levels when compared with group 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MATERIAL AND METHODS : We investigated the secretion of interleukin ( IL ) 1beta , IL 2 , IL 6 , IL 8 , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) and quantified the appropriate expression of corresponding mRNA in PC with regard to different levels of WBC contamination and storage times . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Significant levels of interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) were secreted in the presence of IL 2 and IL 15 , but not in the presence of IL 21 , demonstrating that proliferating phosphoantigen reactive Vgamma9 / Vdelta2 T cells do not necessarily produce proinflammatory cytokines . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In comparison with control animals , the suppression of PG synthesis produced higher inflammation and expression of TNF alpha , interleukin 1alpha and interferon gamma ( IFN gamma ) , but almost complete disappearance of iNOS expression , which coexisted with a significant increment of bacterial load . ^^^ The late progressive phase in this experimental model is characterized by progressive pneumonia , small granulomas and diminished expression of IFN gamma , TNF alpha and iNOS in coexistence with high expression of IL 4 . ^^^ When PG production was suppressed in animals suffering advanced phase infection , a significant reduction of pneumonia and bacillus load with striking increment of granuloma size was seen , and the expression of IFN gamma , TNF alpha and iNOS was also improved . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A comparison of intrahepatic cytokine levels in Ch 1552 and Ch 1605 showed greater and earlier gamma interferon ( IFN gamma ) and tumor necrosis factor alpha responses in the previously infected animal , responses that were 30 fold greater than baseline responses at week 4 p . i . for IFN gamma in Ch 1552 compared to 12 fold in Ch 1605 at week 10 p . i . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Plasmid DNA standards of each marker ( CD 3 , CD 4 , CD 8 , IL 2 , IFN gamma , TNF alpha , IL 4 , IL 10 ) , and RNA standards from mitogen stimulated woodchuck peripheral blood mononuclear cells ( PBMCs ) were used to validate and optimize the assays for TaqMan 7700 and iCycler PCR instruments . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Auto PBSCT reduced the abdominal lymphoma mass and IFN alpha eliminated residual tumor cells , possibly through the induction of specific T cell subsets expressing CD 3 , CD 8 on their surfaces and either IFN gamma or tumor necrosis factor ( TNF ) alpha in cytoplasm . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Survival time and parasite persistence were examined in interferon gamma ( IFN gamma ) , tumor necrosis factor receptor 2 ( TNFR 2 ) , interleukin 10 ( IL 10 ) , beta 2 microglobulin ( beta2M ) , and inducible nitric oxide synthase ( iNOS 2 ) gko or wild type ( wt ) mice following infection with either pathogenic ( NC 1 or NC 2 ) or attenuated ( NCts 8 ) N . caninum strains . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
ASC expression was transiently up regulated by IL 1alpha , IL 1beta , IFN alpha , IFN gamma , TNFalpha , and LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Myelosuppressive cytokines , in particular IFN gamma and TNF alpha , play an important role in the pathogenesis of idiopathic aplastic anemia in humans . ^^^ In this study , we provide evidence that a common element in signaling for IFN gamma and TNF alpha in human hemopoietic progenitors is the p38 / MapKapK 2 signaling cascade . ^^^ Our studies indicate that pharmacological inhibition of p 38 reverses the suppressive effects of IFN gamma and TNF alpha on normal human bone marrow derived erythroid and myeloid progenitors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Within 48 h MDA 7 / IL 24 induced secretion of high levels of IL 6 , TNF alpha , and IFN gamma and low levels of IL 1beta , IL 12 , and GM CSF from human PBMC as measured by ELISA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Upon restimulation of effector cells , IL 2 secretion and to a lesser extent TNF alpha expression , but not IFN gamma secretion , were diminished with age . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma inducible T cell alpha chemoattractant is a potent stimulator of normal human blood T lymphocyte transendothelial migration : differential regulation by IFN gamma and TNF alpha . ^^^ However , because most memory T lymphocytes are also CXCR 3 ( + ) , the ability of 1 TAC to promote the migration of normal human blood T cells across HUVEC monolayers in Transwell chambers was examined . 1 TAC induced a marked ( 4 to 6 fold ) increase in transendothelial migration ( TEM ) of T cells across unstimulated HUVEC from 5 . 6 to 28 % of input T cells and was substantially more active than IFN gamma inducible protein 10 , another CXCR 3 ligand . 1 TAC significantly enhanced TEM of T cells across TNF alpha , but not across IFN gamma or IFN gamma plus TNF alpha activated HUVEC . ^^^ IFN gamma or IFN gamma plus TNF alpha activated HUVEC produced substantial amounts of 1 TAC , in contrast to TNF alpha treated EC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the ileum , IFN gamma , TNF alpha , and IL 1beta were induced mainly by the virulent strain , whereas IL 18 was induced in highest quantity by non virulent Salmonella . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Enhanced TNF alpha and IL 1beta mRNA expression was also demonstrated by RT PCR , which revealed transcription of interferon gamma ( IFN gamma ) , IL 12 , and related T cell specific chemokine genes , KC and IP 10 , in the MCP 1 treated macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In our laboratory convincing evidence has been obtained that NA released non synaptically [ 90 , 92 ] from sympathetic axon terminals and enhanced in concentration in the close proximity of immune cells is able to inhibit production of proinflammatory ( TNF alpha , IFN gamma , IL 12 , IL 1 ) and increase antiinflammatory cytokines ( IL 10 ) in response to LPS [ 25 , 91 ] , indicating a fine tuning control of the production of TNF alpha and other cytokines by sympathetic innervation under stressful conditions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Day 21 evaluation showed higher concentrations of nitrate and TNF alpha soluble receptors in C57BL / 6 mice with no differences in IFN gamma levels , with respect to the BALB / c group . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumour inoculation induced secretion of IL 2 , IL 12 , IFN gamma and TNF alpha by tumour infiltrating mononuclear cells ( TIM ) in ascitic fluid as well as in serum . ^^^ The levels of IL 2 , IL 12 , IFN gamma and TNF alpha secretion were higher in animals , which rejected tumours as compared with the animals that failed to reject the tumours . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In reporter gene assays , T cell lines transfected with the variant 204 ( 179T ) IFN gamma promoter show a six to 13 fold induction of luciferase activity in response to TNF alpha over the common 204 ( 179G ) construct . ^^^ The 179T allele identified in the proximal IFN gamma promoter confers TNF alpha inducibility and may prove important in human immune disorders and responsiveness to pathogens . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Graft survival , histological appearance and intragraft gene expression ( IL 2 , IFN gamma , IL 12 p 40 , IL 4 , IL 10 , perforin , Fas ligand ( Fas L ) , iNOS and TNF alpha ) were compared for fully allogeneic , minor histocompatibility ( mHC ) antigen mismatched and syngeneic skin and heart grafts . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Results of adoptive transfer experiments suggest that TNF alpha is not involved in LCMV induced wasting disease and show that IFN gamma contributes to the disease . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We show in this work that SOCS 1 , SOCS 2 , SOCS 3 , and cytokine inducible SH 2 containing protein mRNA were up regulated by IFN gamma in normal human keratinocytes , whereas only SOCS 1 or SOCS 1 and cytokine inducible SH 2 containing protein were induced by TNF alpha or IL 4 , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The severity of inflammation corresponded to increased numbers of CD8alphabeta ( + ) NK1 . 1 ( + ) T cells and levels of production of the Th 1 type cytokines IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The antigen ( Ag ) specific effector CD 8 ( + ) T cell population present 2 weeks after boosting had ex vivo Ag specific cytolytic activity , expressed both gamma interferon ( IFN gamma ) and tumor necrosis factor alpha , and constituted 12 to 20 % of splenic CD 8 ( + ) T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The degenerative effect of a single intranigral injection of LPS on the dopaminergic system is prevented by dexamethasone , and not mimicked by rh TNF alpha , IL 1beta and IFN gamma . ^^^ To address the importance of the inflammatory response in the LPS induced degeneration of nigral dopaminergic neurones , we carried out two different kinds of studies : ( 1 ) the possible protective effect of an anti inflammatory compound , and ( 2 ) the effect of the intranigral injection of inflammatory cytokines ( TNF alpha , IL 1beta and IFN gamma ) on dopaminergic neurones viability . ^^^ This damage may be due , at least in part , to a cascade of events independent of that described for TNF alpha / IL 1 beta / IFN gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using synthetic RNA standards , we quantified mRNAs of IL 2 , IL 4 , IL 6 , IL 10 , IL 12 p 40 , interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , RANTES , macrophage inflammatory protein 1 alpha ( MIP 1 alpha ) , and MIP 1 beta in unstimulated peripheral blood mononuclear cells ( PBMCs ) and lymph nodes from macaques chronically infected with SIV or SHIV . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Bacterial DNA with unmethylated CpG DNA stimulates vertebrate immature immune cells to induce maturation and to produce TNF alpha as well as Th 1 type cytokines , IL 12 and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
BDI sum scores ( 2 , 9 fold ) and the expression levels of tumor necrosis factor alpha ( TNF alpha ; 4 fold ) , interferon gamma ( IFN gamma ; 4 , 6 fold ) and interleukin 10 ( IL 10 ; 6 , 1 fold ) mRNA were increased in MS patients during an acute attack compared to age and sex matched healthy controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Transcription of tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) was assessed by multiplex reverse transcription polymerase chain reaction ( RT PCR ) Statistical analysis was performed using the Student ' s t test . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Correlation between the dose and the anti Toxoplasma effect of activated mouse macrophages induced by IFN gamma and the synergism between IFN gamma and TNF alpha ] . ^^^ AIM : To find out the dose dependence of the anti Toxoplasma effect induced by IFN gamma and to determine the possible synergistic activity between TNF alpha and IFN gamma . ^^^ METHODS : The in vitro effect of cultivated mouse peritoneal macrophages activated by IFN gamma alone or IFN gamma combined with different doses of TNF alpha on the intracellular tachyzoites of RH strains and the nitric oxide ( NO ) level in the culture medium supernatant were simultaneously determined . ^^^ CONCLUSION : The anti Toxoplasma effect of macrophages activated by IFN gamma appears to be dose dependent and TNF alpha acts synergitically with IFN gamma in the activation of macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we investigated the mRNA expression of a chemokine , thymus and activation regulated chemokine ( TARC ) , and cytokines including IL 1beta , IL 4 , IFN gamma and TNF alpha in skin samples obtained from both dogs with atopic dermatitis ( AD ) and healthy dogs . ^^^ The expression levels of IL 1beta , IFN gamma and TNF alpha in the lesional skin were also significantly higher than those in the non lesional skin of the dogs with AD . ^^^ The present results suggest that TARC and inflammatory cytokines such as IL 1beta , IFN gamma and TNF alpha may play roles in the pathogenesis of canine AD as well as that of human AD . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of inoculated tumors with TNF and IFN gamma leads to regression and a highly reduced toxicity in ( C57BL / 6 10 SPRET / Ei ) F ( 1 ) mice . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The complex actions of these numerous and active cells , consist in : 1 ) placental regulation and control , based on the receptors for the HLA G and HL C antigens , that are trophoblast specific and act as universal inhibitors of the NK cells ; 2 ) secretion of a large range of cytokines as IL 1 , TNF alpha , IL 8 , TGF , IFN gamma , GM CSF , CSF 1 which regulate the trophoblastic proliferation , differentiation and invasion ; 3 ) removal of abnormal trophoblastic cells , which may appear in such a proliferating cell population ; 4 ) infectious protection of the placental unit , due to their unspecific cytotoxic effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Sera from patients before the first and after the last treatment cycle and from normal donors were assayed by ELISA for IL 2 , IL 1beta , IFN gamma , GM CSF , IL 6 , TNF alpha , and PGE 2 levels . ^^^ Significant differences were observed in the following immune parameters between the control group of healthy blood donors and the pretreatment values of both taxane groups ; IL 2 , GM CSF , IFN gamma levels and NK and LAK cell cytotoxicity were depressed , whereas TNF alpha and IL 6 levels were raised in breast cancer patients before treatment compared to controls . ^^^ Both drugs led to increases in MLR values , NK and LAK cell cytotoxicity , and IL 6 , GM CSF , IFN gamma levels , and decreases for IL 1 , TNF , and PGE 2 levels . ^^^ More specifically , docetaxel demonstrated a more pronounced effect on enhancing MLR , NK , LAK activity and IFN gamma , IL 2 , IL 6 , and GM CSF levels , as well as caused more potent reduction in IL 1 and TNF alpha levels when compared to paclitaxel . ^^^ The present study indicates that patients responded to treatment of advanced breast cancer with single agent paclitaxel or docetaxel leads to an increase in serum IFN gamma , IL 2 , IL 6 , GM CSF cytokine levels and enhancement of PBMC NK and LAK cell activity , while they both lead to a decrease of acute phase serum cytokine levels of IL 1 and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stromal cells are thought to produce increased quantities of inhibitory cytokines such as TNF alpha , TGF beta , IFN gamma et IL 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here we show tumor necrosis factor related apoptosis inducing ligand ( TRAIL ) , which is a type 2 membrane protein belonging to the TNF family and plays a critical role in the NK cell mediated and IFN gamma dependent suppression of subcutaneous growth of TRAIL sensitive tumors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using freshly isolated peripheral blood mononuclear cells , we demonstrate that p 17 was able to enhance levels of tumor necrosis factor alpha and IFN gamma released from cells stimulated by IL 2 . ^^^ IL 4 was found to down regulate IFN gamma and tumor necrosis factor alpha , and p 17 restored the ability of cells to produce both cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast to IFN gamma , however , TNF alpha and IL 8 levels also were elevated in sputum from pneumonia controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The results of exogenous TNF alpha treatment and neutralization treatment with anti TNF alpha and anti gamma interferon ( IFN gamma ) antibodies and the determination of IFN gamma mRNA levels indicate that CSC suppressed macrophages can be activated by EGCg to inhibit L . pneumophila growth by up regulation of TNF alpha and IFN gamma production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These data suggest that the observed differences in IL 10 responses between G6PD deficient and non deficient patients are not attributable to differences in TNF alpha or IFN gamma production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Accordingly , both tapasin mRNA and protein were strongly induced in a time and dose dependent manner in embryonic fibroblasts treated with the cytokines IFN gamma and IFN beta , and weakly induced after treatment with TNF alpha . ^^^ Co stimulation of tapasin by TNF alpha and IFN gamma resulted in a weak synergistic effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After i . v . injection , ( 51 ) Cr labeled T lymphocytes migrated to lesions induced by IFN gamma and tumor necrosis factor ( TNF ) alpha , and in even greater numbers to the combination of IFN gamma + TNF alpha , and to sites injected with concanavalin A ( Con A ) . ^^^ In E selectin mAb treated and in E selectin deficient mice , IFN gamma , IFN gamma + TNF alpha and Con A induced T cell accumulation was inhibited by 45 65 % , but TNF alpha induced infiltration was unaffected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine regulation of periportal fibrosis in humans infected with Schistosoma mansoni : IFN gamma is associated with protection against fibrosis and TNF alpha with aggravation of disease . ^^^ Multivariate analysis of cytokine levels showed that high IFN gamma levels were associated with a marked reduction of the risk of fibrosis ( p = 0 . 01 ; OR , 0 . 1 ) ; in contrast , high TNF alpha levels were associated with an increased risk ( p = 0 . 05 ; OR , 4 . 6 ) of periportal fibrosis . ^^^ These results with observations in experimental models strongly suggest that IFN gamma plays a key role in the protection of S . mansoni infected patients against periportal fibrosis , whereas TNF alpha may aggravate the disease . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 10 , and TNF secretion was also reduced , whereas IFN gamma and IL 5 production was not affected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There was little contribution of cytokines , including IFN gamma , TNF alpha , and IL 12 , to the expansion of CD8+ T cells by live BCG infected DCs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha and IFN gamma induced TER decreases of 22 % + / 3 % that were associated with a 172 % + / 1 % increase in MLC phosphorylation . ^^^ CONCLUSIONS : PIK can prevent TER defects induced by EPEC and reverse MLC phosphorylation increases and TER decreases induced by TNF alpha and IFN gamma . ^^^ The data also suggest that TNF alpha and IFN gamma regulate TER , at least in part , via the perijunctional cytoskeleton . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tissue levels of MIF , tumor necrosis factor ( TNF ) alpha , interferon gamma ( IFN gamma ) , interleukin ( IL ) 4 , and matrix metalloproteinase ( MMP ) 13 messenger RNA ( mRNA ) were measured . ^^^ DSS induced up regulation of colonic TNF alpha and IFN gamma were significantly suppressed in animals given the anti MIF antibody . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After renal transplantation , urinary protein and IL 1beta levels were significantly elevated in recurrent transplanted kidney groups compared with those in the control groups , while the TNF alpha , IL 2 , and IFN gamma levels were not elevated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Sandwich enzyme linked immunosorbent assays ( ELISAs ) were developed for determination of the human cytokines interleukin 4 ( IL 4 ) , interleukin 5 ( IL 5 ) , interferon y ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^ The LOD was 0 . 12 microg / l , 0 . 0077 microg / l , 0 . 0069 microg / l and 0 . 0063 microg / l for IL 4 , IL 5 , IFN gamma and TNF alpha , respectively . ^^^ The combined relative standard uncertainty ( U ( result ) / C ( result ) ) was 28 % , 22 62 % , 28 % and 24 % for the IL 4 , IL 5 , IFN gamma and TNF alpha results , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines ( IL 1 , IL 6 , IL 8 , IL 11 , TNF , IFN gamma , and TGF beta ) and growth factors ( EGF , bFGF , aFGF , and KGF ) play an important role in modulation of hormone secretion by directly influencing specific enzyme steps of steroidogenesis in various endocrine cell types . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha and OSM , only when applied together , increased ALP activities and in vitro calcification in HVSMCs in the presence of IFN gamma and 1 , 25 ( OH ) 2D3 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Hyperosmotic medium also inhibited the expression of IRF 1 induced by TNF alpha or IFN gamma . ^^^ Furthermore , hyperosmotic medium inhibited TNF alpha or IFN gamma induction of guanylate binding protein 1 , another IRF 1 dependent gene . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
BLC expression was also induced in bone marrow derived DC when they were differentiated into mature DC in the presence of TNF alpha and IL 1beta , while both IFN alpha and IFN gamma failed to induce BLC expression in bone marrow derived DC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The role of perforin , Fas / Fas ligand ( FasL ) , tumour necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) in T cell induced mucosal damage was assessed using selected immunodeficient mouse strains . ^^^ Mucosal damage was unaltered in anti CD 3 treated mice lacking IFN gamma , Fas , or TNF alpha receptors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A blockade of LTB ( 4 ) or PAF receptors partially inhibited IFN gamma induced NO and TNF alpha production but not parasite killing . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the effects of the inflammatory cytokines interleukin 1beta ( IL 1beta ) , tumor necrosis factor alpha ( TNF alpha ) , and gamma interferon ( IFN gamma ) on the interaction of M . haemolytica LKT with bovine peripheral blood neutrophils ( PMNs ) . ^^^ In this study we demonstrated , by flow cytometry , that bovine PMNs increased their binding to an anti bovine LFA 1 monoclonal antibody ( BAT75A ) following in vitro incubation with IL 1beta , TNF alpha , or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In rats infected with T . b . brucei we have examined possible associations of the endotoxin increases with increases in levels of TNF alpha , IL 1beta , IL 6 , IFN gamma and nitric oxide ( NO ) . ^^^ The increases in cytokines were highly correlated with the endotoxin levels ( e . g . at day 21 p . i . the correlation regression values were as follows : TNF alpha , r = 0 . 9 , P < 0 . 01 ; IL 1beta , r = 0 . 83 , P < 0 . 01 ; IL 6 , r = 0 . 9 , P < 0 . 01 ; IFN gamma , r = 0 . 7 , P < 0 . 01 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To address that question , we studied the inheritance patterns for polymorphisms in several cytokine genes ( IL 2 , IL 6 , IL 10 , TNF alpha , TGF beta , and IFN gamma ) within an ethnically diverse study population comprised of 216 Whites , 58 Blacks , 25 Hispanics , and 31 Asians . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunohistochemical analysis of the TS / A IL 12 / IL 15 tumor rejection area in IFN gamma deficient mice showed a marked reactive cell infiltration constituted of CD8+ cells , granulocytes , NK cells , macrophages and dendritic cells associated with the expression of IL 1beta , TNF alpha , GM CSF , MCP 1 and MIP 2 . ^^^ These data show IFN gamma independent synergistic anti tumor effects of IL 12 and IL 15 , involving CD8+ cells and secondary chemokines and cytokines , such as TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inflammatory bowel disease ( IBD ) is characterized by a dysregulated intestinal immune response with elevated levels of the Th 1 cytokines TNF , IL 12 and IFN gamma . ^^^ Colonic expression of IFN gamma and TNF was elevated ( 200 and 150 fold , respectively ) and IFN gamma and IL 12 secretion from lymph node cells was increased 5 , 000 and 8 fold , respectively , compared to GpG ODN treated controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Secretions of interferon gamma ( IFN gamma ) and tumor necrosis alpha ( TNF alpha ) , due to Concanavalin A ( Con A ) stimulation , were increased by Rauwolfia radix in activated T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Four antibodies specific for IL 4 , IL 8 , IFN gamma and TNF alpha cross reacted with cytokines from a majority of the species investigated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The present study was to investigate the effect of a calcium antagonist amlodipine on nitrite , tumor necrosis factor alpha ( TNF alpha ) and interleukin 1beta ( IL 1beta ) formation and iNOS induction both in lipopolysaccharide ( LPS ) and interferon gamma ( IFN gamma ) treated rat aortic smooth muscle cells ( RASMC ) and in a rat model of endotoxemia . ^^^ Incubation with amlodipine ( 0 . 1 10 microM ) for 24 h resulted in a significant and dose dependent attenuation in medium nitrite , TNF alpha and IL 1beta formation as well as iNOS protein expression in LPS / IFN gamma treated RASMC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of PBMC with either PIII or P 24 caused significant decrease in cellular proliferation and granuloma formation induced by S . mansoni antigens , and a significant elevation in IL 10 and TNF alpha but not in IFN gamma production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) are important for the pathogenesis of Helicobacter pylori associated gastritis and peptic ulcer disease . ^^^ Gastric biopsies from H . pylori positive and negative patients were used to examine the in situ correlation of TNF alpha and IFN gamma with epithelial cell apoptosis , bacterial load , and histological parameters of gastritis . ^^^ We found a highly significant correlation of TNF alpha and IFN gamma production with activity and grade of gastritis ( P < 0 . 01 ) , H . pylori density ( P = 0 . 01 ) , epithelial cell apoptosis ( P < 0 . 001 ) , and Fas / Fas ligand expression ( P < 0 . 001 ) . ^^^ With the use of serial histological sections , this study showed for the first time the in situ correlation of TNF alpha and IFN gamma with epithelial cell apoptosis , bacterial load , and histological severity of disease and emphasizes the role of these cytokines in the pathophysiology of H . pylori associated disease . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
ELISA was employed to determine before onset of therapy the serum concentrations of IL 12 , IL 12p40 , IFN gamma , IL 4 , IL 10 , tumor mecrosis factor alpha ( TNF alpha ) , TNFR p 55 and p 75 , as well as on days 3 , 5 , 8 , 10 , 12 , 15 , 17 , 22 after onset of therapy and subsequently weekly until the end of the treatment period and at the end of the 12 months ' postobservatory period . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increased numbers of IL 4 expressing cells and decreased numbers of IFN gamma and TNF alpha expressing cells were found in the PNS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There was a synergistic killing effect when recombinant TNF alpha was combined with PHA supernate and this effect could be partly neutralized by monoclonal anti TNF alpha , interleukin ( IL ) 2 , IL 12 and IFN gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Significantly higher levels of tumor necrosis factor alpha ( TNF alpha ; 10 5 . 1 ) , interferon gamma ( IFN gamma ; 10 4 . 8 ) and interleukin 10 ( IL 10 ; 10 5 . 6 ) mRNA were detected in MS patients at the time of a relapse compared to healthy controls . ^^^ Treatment with i . v . methylprednisolone ( MP ) led to an increase of IL 4 mRNA and a significant decrease of IFN gamma and TNF alpha mRNA expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Gamma interferon ( IFN gamma ) is one of the proinflammatory cytokines as well as TNF alpha is critical in activation of endothelial cells . ^^^ Moreover , when mice were injected with 133 ng of Stx 1 or 3 . 3 ng of Stx 2 , survival rates of IFN gamma deficient mice and TNF alpha deficient mice were significantly higher than that of wild type mice . ^^^ The present study using cytokine gene knockout mice directly demonstrated that not only TNF alpha but also IFN gamma is involved in lethality of Stx 1 and Stx2 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using RT PCR and semi quantitative PCR , mRNA expression for canine interferon ( IFN ) gamma , interleukin ( IL ) 2 , IL 4 , IL 5 , IL 10 , tumor necrosis factor ( TNF ) alpha and transforming growth factor ( TGF ) beta in peripheral blood mononuclear cells ( PBMCs ) was examined in dogs with or without demodicosis . mRNA expression for IFN gamma as well as TNF alpha in dogs with demodicosis ( localized ( LD ) and generalized ( GD ) ) was slightly lower than those in dogs without demodicosis ( healthy controls ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Rat vascular smooth muscle ( VSM ) cell and human umbilical vascular endothelial cell ( HUVEC ) cultures were used to determine whether pretreatment with 30 min of isoflurane decreases cell death from tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 ( IL 1 beta ) , and interferon ( IFN gamma ) alone or in combination . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum IL 2 , IFN gamma and TNF alpha levels were increased in most patients in the experimental group , while no differences were observed in Ab 1 ' and cytokine levels between pre and post therapy in the control group . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , cAMP primed CD45RA cells produce considerable amounts of the Th 2 cytokines , IL 4 , IL 10 , and IL 13 , whereas the production of IFN gamma and TNF alpha was nearly undetectable . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Consistent with this effector phenotype , CLA ( + ) / CCR10 ( + ) memory CD 4 T cells from normal donors secrete TNF and IFN gamma but minimal IL 4 and IL 10 following in vitro stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , the addition of IFN gamma and TNF alpha to toxin A stimulated Caco 2 cells increased the basolateral release of CC chemokine MCP 1 . ^^^ In contrast , IFN gamma and TNF alpha had no effect on the expression of the CXC chemokines IL 8 and GRO alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The levels of IL 12 , IFN gamma , TNF alpha and NO were higher for the PAGA / pmIL 12 complexes than those for the naked pmIL 12 , PAGA alone and 5 % glucose injected groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Intravenous administration of a lipoplex triggers high levels of proinflammatory cytokine production , such as TNF alpha , IFN gamma and IL 12 in serum and a large amount of ( 32 ) P labeled lipoplex accumulates in the liver 1 h after intravenous administration . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We measured cellular apoptosis and the cytokines TNF alpha , IFN gamma and IL 10 . ^^^ Yoghurt increased the number of apoptotic cells and induced IFN gamma and TNF alpha cytokine release , their production being regulated by an increase in IL 10 ( P < 0 . 001 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Patients with progressive disease had a significantly increased number of T cells spontaneously producing IL 2 , IL 4 and GM CSF as compared to healthy donors and patients with non progressive CLL , which was not the case for TNF alpha and IFN gamma producing T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , we analyzed chemokine serum levels ( monocyte chemoattractant protein [ MCP ] 1 , macrophage inflammatory protein [ MIP ] 1alpha , MIP 1beta , and RANTES [ regulated on activation , normal T cell expressed and secreted ] ) and phytohemagglutinin ( PHA ) stimulated cytokine secretion of Th 1 ( gamma interferon [ IFN gamma ] and tumor necrosis factor alpha [ TNF alpha ] ) and Th 2 ( interleukin [ IL ] 4 and 10 ) associated cytokines by PBMC . ^^^ This reduction correlated with reduced IFN gamma and TNF alpha production of PBMCs after PHA stimulation and reversed 6 12 months after diagnosis to normal levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Myoblasts constitutively produced a low level of IL 15 and the production was augmented by stimulation with IFN gamma , IL 1alpha , IL 1beta , TNF alpha or lipopolysaccharide ( LPS ) in a dose dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Spontaneous abortions in the CBA / J 10 DBA / 2 can be boosted by injecting TNF alpha + IFN gamma , but the boosted abortion rates can range from < or = 30 to > 80 % , depending on the loss rate in uninjected mice , and this is not explainable by the endogenous level of these cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine production in the SCD sera group showed increased production of IFN gamma and TNF alpha in the absence of penicillin , but suppression at all doses of penicillin . ^^^ The control group results were as follows : no significant difference in IFN gamma production with or without penicillin , mean TNF alpha levels were the opposite of SCD sera with lower levels in the absence of penicillin . ^^^ The data suggests that sera from healthy SCD patients and in vitro added penicillin may have a combined suppressive effect on normal lymphocyte in vitro production of IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Truncated peptides bound to MHC class 2 molecules and induced TCR internalization and expression of interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) with decreasing potency . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and granulocyte macrophage colony stimulating factor ( GM CSF ) were estimated in the supernatants generated in the two groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study , we investigated the role of interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) in acute infection and reactivation using IFN gamma gene knockout ( IFN gamma ( / ) ) mice and TNF alpha gene knockout ( TNF alpha ( / ) ) mice . ^^^ The survival rates of wild type C57BL / 6 ( B 6 ) mice , IFN gamma ( / ) mice , and TNF alpha ( / ) mice were 97 % ( 73 of 75 ) , 57 % ( 24 of 42 ) , and 83 % ( 60 of 72 ) , respectively . ^^^ These results suggest that TNF alpha and IFN gamma play a protective role in acute infection with HSV 1 . ^^^ The rates of reactivation in IFN gamma ( / ) mice and TNF alpha ( / ) mice were significantly higher than that in B 6 mice ; 16 % ( 4 of 25 ) showed reactivation in B 6 mice , 47 % ( 9 of 19 ) in IFN gamma ( / ) mice , and 48 % ( 10 of 21 ) in TNF alpha ( / ) mice . ^^^ These results suggest that IFN gamma and TNF alpha play an important role in acute infection and reactivation from latency . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CONCLUSION : These data suggest that known cytokine up regulators of MHC Class 2 genes , i . e . , IFN gamma , IL 4 , and TNF alpha may contribute to the upregulation of graft specific MHC Class 2 antigens during an allograft reaction . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The objective of this study was to assess the role of the Th 1 cytokines ( IL 2 , IFN gamma , TNF alpha , TNF beta in this phenomenon , and detection of Th 1 cytokines in women with RSA . ^^^ Tissue culture supernatant from women with RSA also produced higher concentration of TNF alpha , IFN gamma , TNF beta and IL 2 , than control group . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Hepatocellular damage induced by BCG pretreated plus inflammatory cytokines mixture ( CM ) , which was included TNF alpha , IL 1beta , IFN gamma and LPS in culture medium in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We determined the influence of melatonin ( MLT ) on the induction of interleukin ( IL ) 2 , IL 1 beta , IL 4 , tumour necrosis factor alpha ( TNF alpha ) and gamma interferon ( IFN gamma ) on mice infected with the Venezuelan equine encephalomyelitis ( VEE ) virus . ^^^ The blockade of IFN gamma and TNF alpha did not inhibit the protective effect of MLT but when IL 1 beta was neutralized , 100 % of the infected mice died suggesting that IL 1 beta induced by MLT treatment is a target cytokine to generate an immune response against the viral infection . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The frequencies of CD8+ and CD4+ T cells responsive to cytomegalovirus ( CMV ) , varicella zoster virus ( VZV ) , and tetanus in antigen activated whole blood were determined by flow cytometric analysis of CD 69 , TNF alpha , IFN gamma and IL 4 expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Murine peritoneal exudate macrophages ( mPEM ) incubated with the supernatant of infected and stressed INS 1 cells were tested for chemotaxis and cytokine release ( TNF alpha , IL alpha and IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Endothelial cells were activated by inflammatory mediators , such as tumour necrosis factor alpha ( TNFalpha ) , interferon gamma ( IFN gamma ) , and bacterial substance lipopolysaccharide ( LPS ) , and the PAR 1 expression was verified by flow cytometry or RT PCR . ^^^ By stimulating endothelial cells with TNFalpha , IFN gamma , and LPS , the PAR 1 expression on the cell surface remained almost unchanged for 48 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Supernatants from cultured splenocytes and peritoneal cells taken from Linomide treated mice contained lower levels of TNFalpha , IL 1 beta , IFN gamma and IL 12 versus higher levels of IL 4 , IL 6 and IL 10 in comparison with supernatants from cultures of untreated mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Titers of endogenous gamma interferon ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) in the spleens , livers , and lungs were parallel to bacterial numbers in each organ of mice during intranasal infection and intravenous infection . ^^^ IFN gamma deficient mice and TNF alpha deficient mice were highly susceptible to intranasal infection as well as intravenous infection . ^^^ These results suggest that both IFN gamma and TNF alpha play critical roles in host resistance to intranasal L . monocytogenes infection as well as the intravenous infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunoblotting and nitrite assays revealed that C . burnetii infection of L 929 cells augments expression of iNOS up regulated in response to gamma interferon ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^ Treatment of infected cells with IFN gamma and TNF alpha or the synthetic nitric oxide donor 2 , 2 ' ( hydroxynitrosohydrazino ) bis ethanamine ( DETA / NONOate ) had a bacteriostatic effect on C . burnetii replication . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This led us to evaluate the immunomodulatory properties of this plant extract on various in vitro tests such as human natural killer ( NK ) cell cytotoxicity , production of nitric oxide ( NO ) in mouse macrophage cells , RAW 264 . 7 , interleukin 2 ( IL 2 ) , tumor necrosis factor alpha ( TNF alpha ) , intracytoplasmic interferon gamma ( IFN gamma ) and expression of various cell surface markers on human peripheral blood mononuclear cells ( PBMCs ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Instead , IL 4 tended to induce Th 1 cells , defined by production of IFN gamma and TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) are considered as soluble mediators of BM suppression in AA . ^^^ The effect was dose dependent and correlated with decreased IFN gamma and TNF alpha production in suspension cultures . ^^^ Using intracellular cytokine staining it was found that increased TNF alpha production in AA cells was derived from both CD4+ and CD8+ cells , whereas aberrant IFN gamma synthesis was only detected in CD8+ cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Carcinogenesis in OLP may be regulated by the integrated signal from various tumor inhibitors ( TGF beta 1 , TNF alpha , IFN gamma , IL 12 ) and promoters ( MIF , MMP 9 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Complement factor B gene regulation : synergistic effects of TNF alpha and IFN gamma in macrophages . ^^^ The inflammatory cytokines , in particular TNF alpha and IFN gamma , are critical in the regulation of Bf gene expression in macrophages . ^^^ In this study , we investigated the mechanisms of Bf gene regulation by TNF alpha and IFN gamma in murine macrophages . ^^^ Northern analysis revealed that Bf mRNA expression was synergistically up regulated by TNF alpha and IFN gamma in MH S cells . ^^^ Truncations of the 5 ' Bf promoter identified a region between 556 and 282 bp that mediated TNF alpha responsiveness as well as the synergistic effect of TNF alpha and IFN gamma on Bf expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Self specific Ab to IP 10 developed in protected animals , as well as neutralizing Ab to IP 10 that we have generated in rabbits , could inhibit leukocyte migration , alter the in vivo and in vitro Th1 / Th2 balance toward low IFN gamma , low TNF alpha , high IL 4 producing T cells , and adoptively transfer disease suppression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine IL 6 , TNF alpha , sTNF RII , sIL 2R , IL 12 , IL 10 and IFN gamma , and other acute phase reactants as alpha 1 antitrypsin , ferritin , CRP and platelets were all raised in patients , whereas the IL 2 was decreased . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Intragraft expression of CD 62 P selectin was similar in both recipient groups . rPSGL Ig treatment decreased intragraft infiltration by CD3 / CD25 cells , diminished expression of pro inflammatory TNFalpha , IL 6 , iNOS , IL 2 and IFN gamma , without significantly affecting mRNA levels coding for anti inflammatory IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
None of the remaining cytokines , including EGF ; GM CSF ; insulin like growth factor 1 ( IGF 1 ) ; IFN alpha , IFN beta , and IFN gamma ; IL 1 alpha , IL 1 beta , IL 2 , IL 6 , and IL 10 ; leukemia inhibitory factor ( LIF ) ; macrophage colony stimulating factor ( M CSF ) ; platelet derived growth factor BB ( PDGF BB ) ; transforming growth factor beta ( TGF beta ) ; and TNF alpha , affected transcriptional expression of the promoter constructs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma leads to STAT 1alpha activation directly and up regulation of NF kappaB activity due to the secretion and subsequent autocrine signaling of TNF alpha . ^^^ Additionally , IFN gamma induced TNF alpha secretion , as well as STAT 1alpha and NF kappaB activation , are inhibited in the presence of SOCS 1 . ^^^ We conclude that SOCS 1 inhibits cytokine induced CD 40 expression by blocking IFN gamma mediated STAT 1alpha activation , which also then results in suppression of IFN gamma induced TNF alpha secretion and subsequent NF kappaB activation . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CD 8 ( + ) T cell lines to the 18 Cpn epitope bearing peptides were cytotoxic , displayed a memory phenotype , and secreted IFN gamma and TNF alpha , but not IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Remarkably , disruption of smez led to a complete inability to elicit cytokine production ( TNF alpha , lymphotoxin alpha , IFN gamma , IL 1 and 8 ) from human cells , when cocultured with streptococcal supernatants . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The fact that antibodies to IFN gamma also inhibit AgICD suggests that the perforin plus granzyme independent and FaSL and / or TNF alpha facilitated process of AgICD of T effector cells is tightly regulated by endogenous IFN gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
On coculture of `` naive ' ' CD 4 ( + ) T cells with mature DC in the presence of superantigen , ICOS was highly up regulated on T cells , but played only a secondary role in the CD 28 dominated release of TNF alpha and IFN gamma , and did not participate in the induction of IL 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Monocyte IL 10 production increased significantly and monocyte tumor necrosis factor alpha ( TNF alpha ) , IL 1alpha , IL 6 , IL 8 , T cell interferon gamma ( IFN gamma ) , IL 2 , and TNF alpha decreased significantly in the presence of > or=10 microg / ml garlic extract . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Analysis of cytokine expression in s . c . tumors revealed increased mRNA levels of interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and interleukin 6 ( IL 6 ) in treated tumors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
ACD3S expanded CD 3 ( + ) CD 56 ( + ) cells exhibited increased levels of cytoplasmic interleukin 2 ( IL 2 ) , tumor necrosis factor alpha ( TNF alpha ) , gamma interferon ( IFN gamma ) and perforin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , MPA markedly inhibited IFN gamma + LPS triggered astrocyte expression of mRNA for iNOS and its transcription factor IRF 1 , while the expression of tumor necrosis factor alpha ( TNF alpha ) gene was not altered . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We examined the spontaneous production of interleukin 2 ( IL 2 ) , IL 4 , IL 6 , IL 8 , IL 10 , tumor necrosis factor alpha ( TNF alpha ) , and gamma interferon ( IFN gamma ) by peripheral blood B lymphocytes , T cells , CD 8 ( ) T cells , CD 8 ( + ) T cells , CD 3 ( ) CD16 / 56 ( + ) lymphocytes ( natural killer [ NK ] cells ) , CD 3 ( + ) CD16 / 56 ( + ) lymphocytes ( natural T [ NT ] cells ) , and / or monocytes of 316 acutely ill hospitalized persons and 62 healthy adults in Malawi , Africa . ^^^ In patients , spontaneous TNF alpha production occurred most frequently , followed in descending order by IFN gamma , IL 8 , IL 4 , IL 10 , IL 6 , and IL 2 . ^^^ For TNF alpha and IFN gamma , the relationships varied by cell type . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here we show that stimulation of CD 95 in the presence of these inhibitors induces necrosis and expression of various proinflammatory cytokines in primary T lymphocytes , such as TNF alpha , IFN gamma and granulocyte / macrophage colony stimulating factor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
FCA augmented IL 4 , IL 5 and IL 10 production suggesting a strong Th 2 response , while IFN gamma and IL 12 remained low ; poly 1 : C enhanced IFN gamma , IL 12 and TNF alpha eliciting a Th 1 response ; poly A : U resulted in a IL 10 , IL 5 , IL 6 and IL 12 secretion ; and LPS enhanced the IL 10 , IL 6 and TNF alpha production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We demonstrate that MUC 1 expression in T47D breast cancer cells and normal human mammary epithelial cells ( HMEC ) is enhanced by tumor necrosis factor alpha ( TNF alpha ) in the presence of interferon gamma ( IFN gamma ) . ^^^ Collectively , these studies demonstrate synergistic stimulation of MUC 1 expression by TNF alpha and IFN gamma that is mediated by independent actions of NFkappaB p 65 and STAT1alpha upon kappaB and STAT sites , respectively , in the MUC 1 promoter . ^^^ Transient transfection of T47D cells with mutant MUC 1 promoter constructs revealed that a kappaB site at 589 / 580 and the STAT binding element at 503 / 495 and were required for cooperative stimulation by TNFalpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our experiments demonstrated that high initial expression of Bcl 2 protein was significantly downregulated in HUVECs treated with IFN gamma whereas TNF alpha gave a less pronounced decrease in Bcl 2 level . ^^^ Bax protein was upregulated in HUVECs stimulated with TNF alpha alone or in combination with IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After exposure , the authors examined mRNA expression of cytokines ( tumor nocrosis factor alpha [ TFN alpha ] , Interleukin [ IL ] 1beta , IL 4 , IL 6 , IL 10 , IL 12p40 , and interferon gamma [ IFN gamma ] and inducible nitric oxide synthase ( iNOS ) in the lung , and also measured the secretion of TNF alpha and IL 10 protein by alveolar macrophages ( AM ) . ^^^ The mRNA expression levels of inflammatory cytokines ( TNF alpha , IL 1beta , IL 6 , IL 12p40 , IFN gamma ) and iNOS , which are important for host defense , were suppressed significantly . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This occurs in concert with a decrease in the serum levels of anti collagen IgG ( approximately 50 % reduction ) , with relatively more IgG2b and IgG 3 , decreased serum levels of TNFalpha and IFN gamma , and decreased production of those same cytokines after restimulation of lymphocytes in vitro with collagen . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increased expression of TRAIL on tumor cells was observed by flow cytometry after cytokine stimulation ( IFN gamma , TNF alpha ) or the addition of chemotherapeutic agents ( camptothecin , doxolubicin hydrochloride ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Concomitant with exacerbated disease in IL 4 ( / ) mice , there is a significant increase in the systemic production of proinflammatory cytokines IL 12 , TNF alpha , and IFN gamma and in levels of mRNA transcripts for proinflammatory cytokines and chemokines in joints . ^^^ The decrease in disease severity in IFN gamma ( / ) and Stat 4 ( / ) mice correlates with diminished TNF alpha levels but there is no switch to a Th 2 type response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The possibility that TJ function may be perturbed by airway inflammation originated from studies reporting ( 1 ) increased levels of the proinflammatory cytokines interleukin 8 ( IL 8 ) , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and IL 1beta in airway epithelia and secretions from cystic fibrosis ( CF ) patients and ( 2 ) abnormal TJ strands of CF airways as revealed by freeze fracture electron microscopy . ^^^ We found that whereas IL 1beta treatment led to alterations in TJ ion selectivity , combined treatment of TNF alpha and IFN gamma induced profound effects on TJ barrier function , which could be blocked by inhibitors of protein kinase C . ^^^ CF bronchi in vivo exhibited the same pattern of expression of TJ associated proteins as cultures exposed in vitro to prolonged exposure to TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Notably , the cytokines IFN gamma and TNF are strongly expressed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found that tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) and to a lesser extent interleukin ( IL ) 10 production was inhibited by all PGE subtypes in ConA stimulated PBMC concomitant with unaffected IL 2 levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Induction of maturation in DC was obtained by stimulation with TNF alpha , LPS , IFN gamma , CD 40 ligand , or different combinations of these stimuli . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To determine the timing of T cell infiltration and cytokine production in the brain of H 129 infected mice , adjacent , frozen sections of the brain were immunostained for virus , T cells , IL 2 , TNF alpha or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We compared the response of bovine monocyte derived macrophages to ingestion of M . avium subsp . paratuberculosis and M . avium subsp . avium organisms by determining organism survival , superoxide and nitric oxide production , and expression of the cytokines tumor necrosis factor alpha ( TNF alpha ) , gamma interferon ( IFN gamma ) , interleukin 8 ( IL 8 ) , IL 10 , IL 12 , and granulocyte monocyte colony stimulating factor ( GM CSF ) . ^^^ Compared to macrophages activated with IFN gamma and lipopolysaccharide , macrophages incubated with M . avium subsp . paratuberculosis showed greater expression of IL 10 and GM CSF ( all time points ) and IL 8 ( 72 h ) and less expression of IL 12 ( 72 h ) , IFN gamma ( 6 h ) , and TNF alpha ( 6 h ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
P . gingivalis LPS was shown to enhance IL 12 induction of IFN gamma in T cells in a manner independent from TNF alpha contribution . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Overall , P . brasiliensis F 1 fraction induced human monocytes to release relatively high levels of TNF alpha , which , in combination with IFN gamma , is responsible for the activation of human monocytes for effective killing of P . brasiliensis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Apoptotic cells were then added to glial cells that were untreated or prestimulated with interferon gamma ( IFN gamma ) , interleukin 4 ( IL 4 ) , transforming growth factor beta ( TGF beta ) , or tumor necrosis factor a ( TNF a ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , SEB produced lethal shock associated with liver failure and induced cytokines such as TNF alpha , IFN gamma , and IL 2 in both C3H / HeN and C3H / HeJ mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : HKC cells were exposed to both interferon gamma ( IFN gamma , 200 microg / L ) and tumor necrosis factor alpha ( TNF alpha , 20 microg / L ) and cultured in media containing different concentrations of triptolide for 24 h . ^^^ Class 2 MHC , B 7 1 , B 7 2 , and ICAM 1 levels in HKC cells were significantly increased by the costimulation of IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Addition of BDNF did not modulate the effects of TNF alpha and IFN gamma on HCEC , including TNF alpha induced interleukin 8 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Real time RT PCR was used to assess relative mRNA levels from 12 cytokine and 2 chemokine genes ( IL 1alpha , IL 1beta , IL 2 , IL 4 , IL 5 , IL 8 , IL 10 , IL 12p35 , IL 12p40 , IL 15 , IFN gamma , TNF alpha , MCP 1 , RANTES ) . ^^^ RESULTS : In most C . trachomatis infected synovial tissue samples , high levels of IL 10 mRNA were present , with less mRNA for IL 8 , IL 15 , IFN gamma , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) induced the cultured salivary gland epithelial cells to express HLA class 1 antigens , HLA DR antigens , CD 80 , and ICAM 1 , while tumor necrosis factor alpha ( TNF alpha ) induced the expression of HLA class 1 antigens , CD 80 , CD 86 , and VCAM . ^^^ Cultured salivary gland epithelial cells treated with either IFN gamma or TNF alpha also caused allogeneic lymphocytes to proliferate . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The clone produced IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Among them , IFN gamma and TNF alpha are very important in protective immunity against mycobacterial infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To begin to establish how microglia integrate signals from multiple inflammatory mediators , we examined the effects of interleukin 1beta ( IL 1beta ) , interleukin 6 ( IL 6 ) , tumor necrosis factor alpha ( TNFalpha ) , interferon gamma ( IFN gamma ) , and transforming growth factor beta 1 ( TGFbeta 1 ) on both newborn and bulk isolated adult microglia . ^^^ These studies demonstrated that IL 1beta , TNFalpha , IL 6 , but not IFN gamma increase the expression of Cox 2 , whereas they all increase the expression of HCK and FAK . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Rat insulinoma INS 1 cells were stimulated with IL 1beta , TNF alpha , interferon ( IFN ) gamma , and IL 2 as well as with two combinations of these cytokines ( C 1 : IL 1beta + TNF alpha + IFN gamma ; C 2 : TNF alpha + IFN gamma ) . ^^^ Treatment with IL 1beta , TNF alpha , or IFN gamma alone caused a significant down regulation of IA 2 and insulin mRNA levels in a time and dose dependent manner , whereas IL 2 had no effect . ^^^ In conclusion , we have demonstrated for the first time that IL 1beta , TNF alpha , and IFN gamma exert a strong inhibitory effect on expression of the diabetes autoantigen IA 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Flow cytometry and ELISA showed FasL up regulation and increased IFN gamma synthesis in IL 12 treated T cells , while Fas and TNF R 1 showed little change . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
B 7 H1 is not expressed constitutively by ECs ; however , it is rapidly induced by IFN gamma , and synergistically by IFN gamma and TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , MyD 88 deficient , but not IFN gamma deficient , Kupffer cells could not produce TNF alpha in response to LM in vitro , indicating the importance of MyD 88 dependent TNF alpha production for host defense . ^^^ As TLR 2 KO , but not TLR 4 KO , mice showed partial impairment in their capacity to produce IL 12 , IFN gamma , and TNF alpha , TLR 2 activation partly contributed to the induction of IL 12 mediated IFN gamma production . ^^^ These results indicated a critical role for TLRs / MyD88 dependent IL 12 / TNF alpha production and for IL 12 and IL 18 mediated IFN gamma production in early phase clearance of LM . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Listeria infected MyD 88 deficient mice also showed markedly diminished IFN gamma , TNF alpha , and NO responses , despite evidence of macrophage activation and up regulation of MHC class 2 molecules . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Divergent role for TNF alpha in IFN gamma induced killing of Toxoplasma gondii and Salmonella typhimurium contributes to selective susceptibility of patients with partial IFN gamma receptor 1 deficiency . ^^^ Whereas TNF alpha production was normally up regulated in response to IFN gamma , IL 12 production and CD 64 up regulation were strongly reduced , and IFN gamma mediated killing of the intracellular pathogens Salmonella typhimurium and T . gondii was completely abrogated in patient ' s macrophages . ^^^ IFN gamma induced killing of T . gondii appeared to be partially mediated by TNF alpha , and addition of TNF alpha could compensate for the abrogated killing of T . gondii in the patient ' s macrophages . ^^^ In contrast , IFN gamma mediated killing of S . typhimurium appeared to be independent of TNF alpha . ^^^ We propose that the divergent role of TNF alpha in IFN gamma induced killing of T . gondii and S . typhimurium may at least partially explain the highly selective susceptibility of patients . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of these two preparations on the capacity of staphylococcal toxic shock syndrome toxin 1 ( TSST 1 ) to stimulate the production of cytokines ( IL 1beta , IL 6 , TNF alpha , IFN gamma ) and chemokines ( MIP 1alpha , MIP 1beta and MCP 1 ) by peripheral blood mononuclear cell ( PBMC ) was tested . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , TNF alpha antibodies were found completely to neutralize the IFN gamma induced inhibition in DNA synthesis as well as apoptotic cell death in macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Allo DC primed T cells showed a restricted cytokine profile characterized by the production of TNF alpha , IFN gamma and IL 2 but low to undetectable levels of IL 4 and IL 10 . ^^^ The synthesis of TNF alpha and IFN gamma and the up regulation of OX 40 on T cells after secondary allogeneic stimulation were almost entirely blocked by 10 nm pimecrolimus . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Twelve rats ( 6 per group ) were killed on days 1 , 2 , 7 , 21 , 35 , 56 , and 112 ; mucosa was harvested and assayed for interleukin 1beta ( IL 1beta ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 6 ( IL 6 ) , interleukin 10 ( IL 10 ) , interferon gamma ( IFN gamma ) , transforming growth factor beta ( TGF beta ) , monocyte chemoattractant protein 1 ( MCP 1 ) , and interleukin 8 ( IL 8 ) gene expression , and effusion was assayed for IL 1beta , TNF alpha , IL 6 , IL 10 , and macrophage inflammatory protein 2 ( MIP 2 ) protein . ^^^ Three patterns of mucosal cytokine messenger RNA ( mRNA ) upregulation were observed : isolated early ( IL 1beta , IL 8 ) , isolated late ( TNF alpha , IFN gamma ) , and biphasic ( MCP 1 , IL 6 , TGF beta ) responses . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Differential regulation of thymus and activation regulated chemokine induced by IL 4 , IL 13 , TNF alpha and IFN gamma in human keratinocyte and fibroblast . ^^^ In order to compare the regulatory profiles of TARC production by tumor necrosis factor alpha ( TNF alpha ) , IFN gamma , interleukin 4 ( IL 4 ) and IL 13 in keratinocytes and fibroblasts , HaCaT cells , a human keratinocyte cell line , and NG1RGB cells , a human skin fibroblast cell line , were used . ^^^ The spontaneous expression of TARC protein and mRNA levels were augmented by TNF alpha and IFN gamma and were inhibited by IL 4 and IL 13 in the keratinocytes . ^^^ Thus , TNF alpha and IFN gamma induced TARC production was differentially regulated by IL 4 and IL 13 in human keratinocytes and fibroblasts . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
As well as the inhibition of rejection which follows blockade of MD 1 expression and / or augmentation of CD 200 expression , an altered polarization in cytokine production is seen , with increased expression of interleukin 4 ( IL 4 ) , IL 10 and transforming growth factor beta ( TGF beta ) , and decreased IL 2 , interferon gamma ( IFN gamma ) and tumor nerosis factor alpha ( TNF alpha ) . ^^^ Proinflammatory T helper 1 ( Th 1 ) cytokines ( TNF alpha + IFN gamma + IL 1 ) have been shown to cause spontaneous abortion in mice by activating a novel prothrombinase , fibrinogen like peptide ( fibroleukin ) fgl 2 , which may promote fibrin deposition in the graft rejection process ; expression of IL 10 , TGF beta , and progesterone induced blocking factor ( PIBF ) in contrast leads to lowering of abortion rates . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study also established that RRV enhanced macrophage phagocytic activity and dysregulated the immunoregulatory molecules CD 80 , IFN gamma , and TNF alpha that may facilitate persistence of RRV and avoidance of immune responses . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Five days after excision and grafting , cardiac function was determined and blood samples were taken for serum levels of IGF 1 , IGFBP 3 , creatinine , pre albumin , cholinesterase , pro inflammatory cytokines ( IL 1beta , IL 6 , and TNF ) , and anti inflammatory cytokines ( IL 2 , IL 4 , IL 10 and IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Eight human cytokine [ interleukin 4 ( IL 4 ) , IL 6 , IL 10 , transforming growth factor beta 1 ( TGF beta 1 ) , monocyte chemotactic and activating factor ( MCAF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) ] gene expression vectors were constructed and injected into rat skin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of mRNA encoding interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) was studied , by reverse transcription polymerase chain reaction and by in situ hybridization with a non radioactive digoxigenin labelled probe , in formalin fixed , paraffin wax embedded lung tissue from pigs experimentally infected with a Korean isolate ( North American genotype ) of PRRSV . ^^^ Both IFN gamma and TNF alpha mRNA were detected in the lung as early as 1 day post inoculation ( dpi ) and up to 10 dpi . ^^^ Hybridization signals for IFN gamma and TNF alpha were usually associated with inflammation , in particular with macrophages within alveolar septa . ^^^ The results suggest that IFN gamma and TNF alpha play a role in pulmonary defence against PRRSV . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF dependent BALB / c murine macrophage apoptosis following Mycobacterium tuberculosis infection inhibits bacillary growth in an IFN gamma independent manner . ^^^ Pre treatment of PM with interferon ( IFN gamma ) resulted in significant killing of the infecting bacilli , which was not dependent on TNF or apoptosis of the cells . ^^^ CONCLUSION : These findings suggest that TNF contributes to macrophage inhibition of M . tuberculosis growth by a mechanism that is dependent on apoptosis and independent of IFN gamma activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , IL 5 , tumor necrosis factor and IFN gamma production , and CD 69 expression by T cells were not depressed in infected SC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Following 1 h of incubation of whole blood with swine dust , CD14+ cells produced high levels of TNF alpha and IL 12 , whereas CD3+ , CD4+ and CD8+ T cells and CD56+ CD16+ NK cells required a longer incubation time ( 22 h ) to produce IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , the alternative program is characterized by selective induction of IFN gamma and TNF alpha , absence of proliferative response and Fas independent cell death . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of synovial TNF , IFN gamma , IL 17 , IL 2 and IL 4 mRNA was unchanged in treated rats and TGF beta expression was significantly increased at day 13 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When the HDC deficient mice were implanted with mutant CT 26 cells stably expressing HDC , drastic suppression of tumor growth by cimetidine was observed , which was accompanied by augmentation of mRNA expression of LT beta , TNF alpha , and IFN gamma in the tumor tissues . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , the levels of cytokines , including tumor necrosis factor alpha ( TNF alpha ) , interleukin 1beta ( IL 1beta ) , IL 6 and Interferon gamma ( IFN gamma ) were increased and the production of reactive oxygen / nitrogen components such as nitric oxide ( NO ) and hydrogen peroxide ( H2O2 ) was enhanced . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The disease is characterized by the development of a T helper cell type 1 like cytokine response [ interleukin ( IL ) 12 , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ] , and macrophage activation is central to development of the syndrome . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : We measured IFN gamma , IL 5 , and TNF alpha production against representative DPs [ gliadin , cow ' s milk protein ( CMP ) , and soy ] by peripheral blood mononuclear cells ( PBMCs ) from ASD and control children [ those with DP intolerance ( DPI ) , ASD siblings , and healthy unrelated children ] . ^^^ RESULTS : ASD PBMCs produced elevated IFN gamma and TNF alpha , but not IL 5 with common DPs at high frequency as observed in DPI PBMCs . ^^^ ASD PBMCs revealed increased proinflammatory cytokine responses with LPS at high frequency with positive correlation between proinflammatory cytokine production with LPS and IFN gamma and TNF alpha production against DPs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A multidonor ELISPOT study of IL 1 beta , IL 2 , IL 4 , IL 6 , IL 13 , IFN gamma and TNF alpha release by cryopreserved human peripheral blood mononuclear cells . ^^^ To understand the effect of cryopreservation , frozen PBMCs from eight healthy donors were studied to release T ( H ) 1 or T ( H ) 2 cytokines including IL 1 beta , IL 2 , IL 4 , IL 6 , IL 13 , TNF alpha and IFN gamma using ELISPOT assay . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Selective disruption of the tumor vasculature by high doses of tumor necrosis factor ( TNF ) and interferon gamma ( IFN gamma ) , and the antiangiogenic activity of nonsteroidal anti inflammatory drugs are associated with the suppression of integrin alpha 5 beta 3 function and signaling in endothelial cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The present study demonstrates that a 24 h treatment with IFN gamma increases interleukin ( IL ) 6 release but reduces IL 8 secretion of both untreated and of tumor necrosis factor alpha ( TNF alpha ) stimulated endothelial cells ( ECs ) , favoring the attraction of lymphocytes but not of neutrophils . ^^^ However , IFN gamma application neither altered gene expression of both TNF alpha receptors ( p 55 and p 75 ) nor cellular density of TNF alpha receptor 2 ( p 75 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Exercise induced a reduction in peripheral blood mononuclear cell proliferation ( 37 % ) as well as in the production of cytokines by cultured cells ( interleukin 1 ( IL 1 ) , interleukin 2 ( IL 2 ) , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) , by 37 % , 35 % , 26 % and 16 % , respectively ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neutralization of IFN gamma , TNF alpha , and IL 1beta was not protective in cytokine treated mice , however , toxicity and death could be completely abrogated by depletion of NK cells . ^^^ These findings suggest that cytokine stimulated NK cells are able to promote systemic inflammation via the induction of STAT 4 responsive genes other than IFN gamma or TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Epithelial permeability , T cell numbers , TNF alpha and IFN gamma mRNA expression , and epithelial proliferation and apoptosis were compared with goblet cell numbers over time . ^^^ Neither TNF alpha nor IFN gamma mRNA expression in the TPN and PEN ileum correlated with goblet cell expansion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The regulation of signal transducer and activator of transcription 1 ( STAT 1 ) by cytokines and all trans retinoic acid ( RA ) was investigated in THP 1 monocytic cells cultured with RA and stimulated with lipopolysaccharide ( LPS ) , tumour necrosis factor alpha ( TNF alpha ) , interferon beta ( IFN beta ) , and IFN gamma , individually or in combinations . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We hypothesize that there may be a down regulation of promoting factors ( nitric oxide ) and , in turn , an up regulation of healing inhibiting factors ( TNF alpha and IFN gamma ) in the wound after radiation . ^^^ Nitrite and nitrate ( index of NO synthesis ) , TNF alpha , and IFN gamma were measured within the wound fluid . ^^^ TNF alpha and IFN gamma were increased in irradiated wounds ( P < 0 . 05 ) . ^^^ CONCLUSION : Radiation impaired healing is reflected in an imbalance of promoting and inhibiting factors , leading to increased levels of TNF alpha and IFN gamma and decreased NO expression in the wound . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Chronic inflammation can modify the process of erythropoiesis , probably mediated via pro inflammatory cytokines such as interleukin 1 ( IL 1 ) , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) . ^^^ Poor responders had significantly reduced CD 28 expression on both CD4+ and CD8+ cells , enhanced IL 10 generation from peripheral blood mononuclear cells ( PBMCs ) , higher plasma IL 12 levels , and increased TNF alpha and IFN gamma release from PBMCs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Accordingly , we investigated the effects of cytomix , a mixture containing IFN gamma , TNF , and IL 1beta on the expression of HIF 1 dependent proteins under normoxic and hypoxic conditions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Because such cytokines as tumor necrosis factor ( TNF ) alpha and interferon gamma ( IFN gamma ) have been shown to enhance RANTES induction in airway epithelial cells and RANTES gene expression appears to be differentially regulated depending on the cell type and the stimulus applied , in this study we have elucidated mechanisms that operate to control RANTES induction on exposure to TNF alpha and / or IFN gamma . ^^^ Our results indicate that TNF alpha and IFN gamma synergistically induce RANTES protein secretion and mRNA expression . ^^^ RANTES transcription is activated only after stimulation with TNF alpha , but not IFN gamma , which affects RANTES mRNA stabilization . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CTLA4Ig treatment in vitro reduced the expression of IL 2 receptor on T cells , enhanced T cell apoptosis and decreased the synthesis of IL 2 , IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The beneficial effects of pCDR 3 on the clinical manifestations of SLE were associated with downregulation of the Th 1 type ( IL 2 , IFN gamma ) and proinflammatory ( TNF alpha ) cytokines , whereas the immunosuppressive cytokine TGF beta was up regulated . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Plasma levels of TNF alpha , IL 8 , IL 6 , IL 1 ra and IL 10 were elevated , whereas IL 2 and IFN gamma , produced predominantly by T lymphocytes , did not change in response to endotoxin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CONCLUSION : Serum concentrations of proinflammatory cytokines ( TNF , IL 1 , IFN gamma and IL 12 ) were not affected by operation or the occurrence of septic complications . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Low interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) production in peripheral blood mononuclear cells ( PBMC ) from patients with atopic dermatitis and food allergy have been reported previously . ^^^ The frequencies of the cells expressing intracellular IL 4 , IFN gamma , and TNF alpha were assessed using flow cytometry . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The antiviral effect depended on the production of IL 12 and TNF alpha by activated APCs ; however , it was mediated primarily by IFN gamma produced by NK cells , and possibly T cells , that were activated by IL 12 . ^^^ Collectively , these results suggest that activated APCs can directly produce antiviral cytokines ( IL 12 , TNF alpha ) and trigger the production of other cytokines ( i . e . , IFN gamma ) by other cells ( e . g . , NK cells and T cells ) that do not express CD 40 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here , we show that the combination of TNF alpha and IFN gamma induces the release of endothelin 1 from human pulmonary artery smooth muscle cells via increased transcription of prepro endothelin 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When cultured in the presence of interferon ( IFN ) gamma , interleukin ( IL ) 1beta , tumor necrosis factor ( TNF ) alpha , IL 2 , or IL 10 , VEGF mRNA expression was found to be significantly increased by IL 1beta , IFN gamma and TNF alpha but to be unaffected by IL 2 and IL 10 . ^^^ CONCLUSION : These results suggest that IL 1beta , IFN gamma , and TNF alpha may regulate the production of VEGF in early gestational trophoblasts . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHOD OF STUDY : Absolute cell counts and percentages of CD3+ , CD3+ / CD4+ , and CD3+ / CD8+ T cell populations expressing intracellular cytokines [ interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin ( IL ) 4 and IL 10 ] was studied by four color flow cytometry in 15 RSA and 13 implantation failure patients . ^^^ CONCLUSION : Absolute counts of IFN gamma , IL 4 , and TNF alpha expressing T cells decrease with the progress of gestation ( third trimester ) during normal pregnancies . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The proliferation was accompanied by enhanced production of several lymphokines , such as IFN gamma , IL 6 , IL 10 , TGF beta , TNF alpha , and GM CSF , but not IL 2 and IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In conclusion , iNOS expression in vivo is dependent on both TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the effect of tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) on the gene expression of 19 different PLA ( 2 ) types ( IB , IIA , IID , IIE , IIF , 3 , IVA , IVB , IVC , 5 , VIA , VIB , VIIA , VIIB , VIIIA , VIIIB , 10 , 12 , and 13 ) in human bronchoepithelial ( BEAS 2B ) and nasal epithelial ( RPMI 2650 ) cells . ^^^ The cells were stimulated with TNF alpha or IFN gamma for different lengths of time ( 1 , 4 , 18 , and 48 h ) , and the mRNA levels of the different PLA ( 2 ) types were determined by reverse transcriptase PCR ( RT PCR ) and normalized to those of the housekeeping gene , GAPDH . ^^^ In both cell lines , TNF alpha increased the expression of PLA ( 2 ) IVA and IVC , and IFN gamma increased the expression of PLA ( 2 ) IIA and IID . ^^^ These findings indicate that TNF alpha and IFN gamma induce gene expression of two novel cytosolic and secretory PLA ( 2 ) types ( IVC and IID , respectively ) in human airway epithelial cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
With minor quantitative differences , the MuGBPs also had similar patterns of response to IFN gamma , lipopolysaccharide ( LPS ) , interleukin 1beta ( IL 1beta ) , and tumor necrosis factor alpha ( TNF alpha ) in RAW 264 . 7 and Swiss 3T3 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Circulating IFN gamma , IL 2 , IL 4 , IL 6 , IL 10 , IL 13 , TNF alpha , TGF beta levels were measured by ELISA in the sera . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The stimulatory activity of MDOC towards the immune system was further supported by the fact that in vitro the product stimulates the release of Th 1 cytokine TNF alpha , but not IFN gamma , IL 4 or IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Transforming growth factor beta 2 ( TGF beta 2 ) was found to be expressed in 8 HBV associated hepatoma cell lines , and to be potently expressed in 5 cell lines ; however , the mRNA expressions of interleukin 10 ( IL 10 ) , IL 12 , interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) were not detected in any cell lines examined . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , several mice with hematopoietic defects had progenitors that displayed a marked resistance to IFN gamma , TNF alpha , and MIP 1alpha compared to both Fancc ( / ) progenitors , which are uniquely hypersensitive to these cytokines , and wild type progenitors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using flow cytometry , we measured the percentage production of intracellular cytokines [ interleukin 1 alpha ( IL 1 alpha ) , IL 6 , IL 12 , tumor necrosis factor alpha ( TNF alpha ) , IL 4 , and interferon gamma ( IFN gamma ) in T lymphocytes from blood and peritoneal fluid . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Reporter gene analysis showed that expression of CBP potentiated IFN gamma and tumor necrosis factor ( TNFalpha ) induced promoter activity and that the CBP mediated synergy depended upon STAT 1 and NF kappaB binding sites in the promoter . ^^^ Furthermore , chromatin immunoprecipitation revealed that , although costimulation with IFN gamma and TNFalpha did not cooperatively enhance the levels of acetylated histones , it did result in increased recruitment of STAT 1 , CBP , and RNA polymerase 2 at the promoter region of the CXCL 9 gene . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : IFN gamma inhibited in a dose dependent manner the release of eotaxin induced by each of the proinflammatory cytokines tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 alpha ( IL 1 alpha ) and IL 1 beta in corneal fibroblasts . ^^^ The synergistic increases in eotaxin release and in eotaxin mRNA abundance induced by the combination of TNF alpha and the T helper cell 2 derived cytokine IL 4 were also both markedly inhibited by the treatment of cells with IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Fewer IFN gamma and TNF alpha producing cells , but more cells secreting IL 4 , were found in sciatic nerve sections from IL 12 / mice , consistent with impaired Th 1 functions and response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Analysis of IL 2 , IFN gamma and TNF alpha production , alpha 5 beta1 integrins and actin filaments distribution in peritoneal mouse macrophages treated with homeopathic medicament . ^^^ To detect the IL 2 , IFN gamma and TNF alpha production these cells were cultivated for 48 h with or without medicament , followed by analyses of these cytokines in supernatant culture with ELISA kits . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The PPS patients displayed increased numbers of CSF cells expressing mRNA for TNF alpha ( p < 0 . 02 ) , IFN gamma ( p < 0 . 02 ) , IL 4 ( p < 0 . 001 ) and IL 10 ( p < 0 . 05 ) , in comparison to the non inflammatory controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Production of IFN gamma , IL 2 , IL 13 , TNF alpha , sCD 23 , and sCD 30 was significantly increased in all B CLL patients compared with controls , whereas there was no difference in IL 4 , IL 6 , IL 10 , and TGF beta production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate the effect of T helper cell type 1 ( TH ( 1 ) ) cytokines [ interferon gamma ( IFN gamma ) , tumor necrosis factor a ( TNF alpha ) ] and T helper cell type 2 ( TH ( 2 ) ) cytokines [ interleukin 4 ( IL 4 ) ] on pathogenesis of intrahepatic cholestasis of pregnancy ( ICP ) . ^^^ TNF alpha , IFN gamma and IL 4 were determined in the supernatant by enzyme linked immunoassay . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TGF beta 1 mediated regulation of thymus and activation regulated chemokine ( TARC / CCL17 ) synthesis and secretion by HaCaT cells co stimulated with TNF alpha and IFN gamma . ^^^ In this paper , the effects of transforming growth factor ( TGF ) beta ( 1 ) on the expression of TARC / CCL17 were examined in HaCaT cells , a human keratinocytes ( KCs ) cell line , co stimulated with TNF alpha and IFN gamma . ^^^ We found that TGF beta ( 1 ) down regulated the TARC synthesis and secretion of HaCaT cells co stimulated with TNF alpha and IFN gamma in a dose dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , by 3 weeks post infection splenocytes from the susceptible BALB / c mice failed to produce IFN gamma and relied on TNF alpha as well as CD 8 T cells to control infection until the end of the plateau phase around 6 weeks post infection when IFN gamma production resumed and clearance began . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The development of this response is under the control of major cytokines like TNF alpha , IFN gamma and IL 12 produced at the onset of infection . ^^^ Under these conditions , they produce TNF alpha and IFN gamma and reduce the bacterial multiplication inside infected autologous macrophages . ^^^ This impairment of B . suis multiplication is due to both soluble factors released from activated gammadeltaT cells ( including TNF alpha and IFN gamma ) and to a contact dependent cytotoxicity directed against the infected cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Proinflammatory cytokines [ e . g . , tumor necrosis factor , IFN gamma , and interleukin ( IL ) 6 ] typically rose within 3 h and were followed at 18 h by a rise in IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The immunoregulatory roles of interleukin 2 ( IL 2 ) , IL 4 , IL 10 , gamma interferon ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , the soluble form of the IL 2 receptor ( sIL 2R ) , and the soluble form of CD 30 ( sCD 30 ) were evaluated in patients with hepatitis B virus ( HBV ) infection . ^^^ IFN gamma and TNF alpha levels decreased in the convalescent phase . ^^^ During the acute phase , IFN gamma and TNF alpha induced increases in IL 2 , sIL 2R , IL 10 , and sCD 30 levels in serum , which allowed the development of immunity characterized by the nonreactivity of the HBV surface antigen , the onset of antibodies to the HBV surface antigen ( anti HBs ) , and normal alanine aminotransferase levels during the convalescent phase . ^^^ The increase in IL 10 levels could result in a decrease in IL 4 levels and modulate IFN gamma and TNF alpha levels during both phases of disease , allowing the maintenance of anti HBs concentrations . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we investigated the effects of terbutaline ( 5 mg ) on IL 10 , IL 12 , IFN gamma and TNF alpha production in whole blood stimulation cultures . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although incubation of HMVEC L monolayers with either TNF alpha or IL 1beta promoted eosinophil migration , IFN gamma had no effect , indicating that enhanced permeability alone was not sufficient for eosinophil infiltration . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of the genes encoding chemokines / cytokines was dramatically changed , such as Mig , IP 10 , RANTES , TNF alpha , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The systemic reaction was evaluated by LD ( 50 ) and the local reaction by edema formation , cellular influx , release of PGE ( 2 ) , NO and H ( 2 ) O ( 2 ) and the production of the pro inflammatory cytokines IL 6 , Tumor necrosis factor alpha ( TNF alpha ) and IFN gamma . ^^^ Coincidently , higher levels of the soluble mediators PGE ( 2 ) , IFN gamma and TNF alpha were detected in the inflammatory exudate induced by BjV in AIR ( max ) mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Several studies reported that IFN gamma and TNF induced the synergistic effect on many cell lines . ^^^ We attempted to clarify the apoptotic activity and the synergistic effect of IFN gamma and TNF on the gastric epithelial cell , and whether IFN gamma relates to soluble TNF receptors ( sTNF R ) release from the gastric epithelial cell . ^^^ METHODS : On the gastric epithelial cell line MKN 45 , cytotoxic and apoptotic effects of IFN gamma and TNF were examined . ^^^ Next , sTNF R released in response to IFN gamma and the protective effect of sTNF R against the cytotoxic activity of TNF and IFN gamma were examined by blocking the release of sTNF R with a serine protease inhibitor such as phenylmethylsulfonyl fluoride . ^^^ Phenylmethylsulfonyl fluoride significantly inhibited shedding of sTNF R and a synergistic effect of TNF and IFN gamma on apoptosis was observed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found that TNF ( / ) T cells do not have intrinsic defects in vitro or in vivo in proliferation , IFN gamma production , or alloactivation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
They comprised 64 % CD 4 ( + ) Th 1 and 36 % CD 8 ( + ) Tc 1 cells , both of which expressed IFN gamma , perforin , and TNF alpha , but not IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Kinetics of TNF alpha and IFN gamma mRNA expression in islets and spleen of NOD mice . ^^^ We utilized a semiquantitative RT PCR assay to analyze in vitro the mRNA expression of TNF alpha and IFN gamma cytokine genes in isolated islets ( N = 100 ) and spleen cells ( 5 10 10 ( 5 ) cells ) from female NOD mice during the development of diabetes and from female CBA j mice as a related control strain that does not develop diabetes . ^^^ An increase in IFN gamma expression in islets was observed for females aged 28 weeks ( 149 + / 29 arbitrary units ( AU ) , P < 0 . 05 , Student t test ) with advanced destructive insulitis when compared with CBA j mice , while TNF alpha was expressed in both NOD and CBA j female islets at the same level at all ages studied . ^^^ The data suggest that IFN gamma and TNF alpha expression in pancreatic islets of female NOD mice is associated with beta cell destruction and overt diabetes . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There is extensive evidence that EAE is a Th 1 disease eliciting secretion of proinflammatory cytokines like IFN gamma or TNF alpha , and it has been suggested that cytokine induced oxidative stress could have a role in EAE neuropathology . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MCP 1 ( monocyte chemotactic protein 1 ) and RANTES are produced during EAT and induced in vitro by IFN gamma , IL 10 , TNF alpha , and IL 1beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , treatment with PT GAG 1 . 5 induced the production of cytokines , interleukin 1beta ( IL 1beta ) , interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) , from murine microphages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , it was tested whether the pro inflammatory cytokines TNF alpha and IFN gamma can regulate cathepsin W gene expression in NK 92 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Elevated intracellular concentrations of interferon gamma ( IFN gamma ) and / or TNF alpha in CD8+ T cells were found in 4 out of 5 patients at 24 h post WBH CT . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 4 Ig was found to downregulate LPS induced IFN gamma and TNF alpha production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 4 , but not IL 13 , modulates TARC ( thymus and activation regulated chemokine ) / CCL17 and IP 10 ( interferon induced protein of 10kDA ) / CXCL10 release by TNF alpha and IFN gamma in HaCaT cell line . ^^^ Both thymus and activation regulated chemokine ( TARC / CCL17 ) , a ligand for CC chemokine receptor 4 that is mainly expressed on Th 2 type cells , and interferon induced protein of 10kDa ( IP 10 / CXCL10 ) , a ligand for CXC chemokine receptor 3 that is mainly expressed on Th 1 type cells , are produced by keratinocytes after the stimulation with the primary cytokines such as tumor necrotic factor alpha ( TNF alpha ) and / or interferon gamma ( IFN gamma ) . ^^^ In this study , we investigated the regulation of TARC or IP 10 production from HaCaT cells , an immortalized human keratinocyte cell line , after stimulation with TNF alpha , IFN gamma , IL 4 and / or IL 13 . ^^^ When both TNF alpha and IFN gamma were added , they increased synergistically ( P < 0 . 003 ) . ^^^ In addition , when HaCaT cells were stimulated with IL 4 , but not IL 13 , in combination with TNF alpha and IFN gamma , the supernatant TARC levels significantly decreased compared to those with both TNF alpha and IFN gamma ( P < 0 . 009 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , IFN gamma potentiates CCL 20 gene expression and production only after 21 h of LPS treatment , its effect being mediated by endogenous TNF alpha in an autocrine fashion , as revealed using neutralizing anti TNF alpha antibodies added to IFN gamma plus LPS treated PMN . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma induced apoptosis and microbicidal activity in monocytes harboring the intracellular bacterium Coxiella burnetii require membrane TNF and homotypic cell adherence . ^^^ IFN gamma mediated caspase 3 activation and C . burnetii killing depend on the expression of membrane TNF . ^^^ Indeed , TNF was transiently expressed on the cell surface of infected monocytes a few hours after IFN gamma treatment . ^^^ In addition , anti TNF Abs inhibited IFN gamma mediated caspase 3 activation whereas soluble TNF had no effect on infected cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Patients with mucosal disease exhibited increased gamma interferon ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) secretion and decreased IL 10 secretion compared to patients with classical cutaneous leishmaniasis . ^^^ CD 4 ( + ) Th 1 cells were the main source of IFN gamma and TNF alpha production in mucosal leishmaniasis patients . ^^^ These data indicate that a poorly modulated T cell response in mucosal leishmaniasis patients leads to production of high levels of proinflammatory cytokines , such as IFN gamma and TNF alpha , as well as a decreased ability of IL 10 and TGF beta to modulate this response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Reactivation was associated with the formation of macrophage dominated lesions , decreased mRNA production for TNF and IL 12p40 , and a decrease in Ag specific IFN gamma secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CT 105 in combination with interferon gamma ( IFN gamma ) elicited a robust and sustained increase of TNF alpha production due to enhanced TNF alpha mRNA transcription , mediated via increased nuclear factor kappaB ( NF kappaB ) in human macrophages derived from monocytic THP 1 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In order to determine whether TNF alpha release was triggered by Th 1 T cell activation by Be stimulation in the context of HLA DPGlu 69 molecules , the proliferation of BeSO 4 stimulated blood mononuclear cells and the release of IFN gamma , TNF alpha , RANTES ( regulated on activation normal T cell expressed and secreted ) , granulocyte macrophage colony stimulating factor , interleukin ( IL ) 4 , IL 6 , IL 8 , IL 10 and IL 12 by BeSO 4 stimulated blood mononuclear cells was quantified in 11 individuals with berylliosis using an anti HLA DP antibody as a probe for HLA DP restricted T cell activation . ^^^ While proliferation and IFN gamma release were completely abrogated by HLA DP inhibition ( inhibition with anti HLA DP monoclonal antibody ( mAb ) : 88+ / 16 and 77+ / 16 % , respectively ; anti HLA DR : 29+ / 38 and 14+ / 10 % , respectively ) , the release of TNF alpha was not ( inhibition with anti HLA DP mAb : 8 . 9+ / 7 . 8 % ) . ^^^ Moreover , Be was able to induce TNF alpha production in healthy control subjects not exposed to Be in the absence of T cell proliferation and IFN gamma production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Genetic polymorphisms were detected by means of polymerase chain reaction in 93 lung allograft recipients for functional polymorphisms in the TNF alpha ( 308 ) , TGF beta 1 ( +869 and +915 ) , IL 6 ( 174 ) , IFN gamma ( +874 ) , and IL 10 ( 1082 , 819 , and 592 ) genes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Indeed , parallel studies with PMN of healthy donors showed that while IFN gamma and granulocyte / macrophage colony stimulating factor ( GM CSF ) induced both , MHC class 2 and CD 83 , tumour necrosis factor ( TNF ) alpha selectively induced de novo synthesis of CD 83 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : IL 10 deficient mice that had been cross fostered to a normal mother demonstrated normal levels of colonic adherent bacteria and reduced TNFalpha and IFN gamma secretion at 2 to 12 weeks of age . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Notably , 2A treatment rapidly augments IFN gamma production , and induces the depletion of autoreactive B cells and abnormal double negative T cells , possibly by increasing their apoptosis through Fas and TNF receptor independent mechanisms . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both orfvIL 10 and ovIL 10 inhibited TNF alpha and IL 8 cytokine production from stimulated ovine macrophages and keratinocytes and IFN gamma and GM CSF production from peripheral blood lymphocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To test whether substance P is involved in immune processes at the blood brain barrier ( BBB ) , we stimulated rat brain endothelial cultures prepared from cerebral microvessels with Interferon gamma ( IFN gamma ) and Tumor necrosis factor alpha ( TNF alpha ) , two proinflammatory cytokines that alter the BBB and measured permeability to albumin and expression of adhesion molecule ICAM 1 and MHC class 2 antigen in the presence and absence of spantide , a powerful substance P antagonist . ^^^ In a dose dependent manner , spantide completely neutralized increased permeability induced by TNF alpha and IFN gamma and expression of MHC class 2 molecule induced by IFN gamma and prevented associated cell morphological changes as revealed by scanning electron microscope . ^^^ Spantide also reduced expression of ICAM 1 induced by TNF alpha and IFN gamma by 35 % and 30 % , respectively . ^^^ Substance P mRNA was found in unstimulated brain endothelial cells and was upregulated after stimulation with TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MOG reactive T cells isolated from both MS patients and healthy subjects exhibited a similar cytokine profile , producing interleukin ( IL ) 4 , IL 10 and tumour necrosis factor ( TNF ) , but not interferon gamma ( IFN gamma ) , and recognized predominantly the extracellular ( residues 1 60 ) and the transmembrane / cytoplasmic ( residues 154 218 ) domains of MOG . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Fluid accumulation was significantly reduced in TNF receptor 1 ( TNFR 1 ( / ) ) , but not IFN gamma knockout mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pro inflammatory cytokines such as IL 1beta , IFN alpha , IFN gamma and TNF alpha affect the 5 HT metabolism directly and / or indirectly by stimulating the enzyme indoleamine 2 , 3 dioxygenase which leads to a peripheral depletion of tryptophan . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Incubation of 0 . 5 ml whole blood with 10 ng / ml E . coli and S . typhosa resulted in a time dependent production of TNF alpha , IL 1beta , IFN gamma , IL 10 and MCP 1 . ^^^ K . pneumoniae , however , showed preferential effects on IL 1beta , IL 10 and MCP 1 production with less potent effects on TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , Vgamma9 / Vdelta2 T cells grown with IL 21 differentiated into putative central memory CD45RO ( + ) T cells that did not produce TNF alpha , IFN gamma , or IL 4 , and maintained expression of CD62L , CD 81 , and CCR7 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Following in vitro restimulation with pMOG ( 35 55 ) , splenocytes harvested from WY 14 , 643 fed mice demonstrate impaired production of IFN gamma , IL 6 , and TNF alpha despite similar proliferative responses . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In immune competent animals , CD 4 ( + ) T cell derived cytokines TNF alpha and IFN gamma mediate vaccine immunity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , when judged by criteria such as interferon gamma ( IFN gamma ) / tumor necrosis factor ( TNF ) production , expression of granzyme , FasL , and NKG2D , the TLGL cells had all the features of a cytotoxic effector T cell . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha mRNA levels did not increase after irradiation ; therefore , mRNA expression of 11 additional cytokines [ interleukin ( IL ) 1 alpha , IL 1 beta , IL 2 , IL 4 , IL 5 , IL 8 , IL 10 , IL 12p35 , IL 12p40 , IL 15 , and IFN gamma ] in both the irradiated and shielded xenografts was quantitated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In initial studies , challenge of splenocytes with TSHR protein induced IFN gamma and tumor necrosis factor alpha ( TNF alpha ) production in all three strains of mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Consequently , we studied luteal immune cells by immunohistochemistry as well as luteal IL 10 , TNFalpha , MCP 1 , IFN gamma , and IL 1beta mRNA expression by semiquantitative RT PCR from day 8 to day 20 in pseudopregnant rabbits ( d 8 d20 p . hCG ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The roles of T lymphocytes and certain cytokines ( TNF alpha , IL 12 , IFN gamma ) in mediating the infection induced expression of ICAM 1 and P selectin were assessed by using relevant mutant mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of the exposure to ELF MFs on the immunological functions of human peripheral blood mononuclear cells ( PBMCs ) obtained from healthy male volunteers were assessed by measuring the natural killer ( NK ) and lymphokine activated killer ( LAK ) activities and the production of interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 2 ( IL 2 ) , and interleukin 10 ( IL 10 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This potentiation of inflammation is explained at least in part by IFN gamma promoting increased sensitivity to TNF alpha and LPS . ^^^ We sought to determine whether and , if so , how IFN gamma can modulate proinflammatory responses to TNF alpha and LPS by epithelial cells , which are key effector cells in the airways . ^^^ Preincubation of airway epithelial like NCI H 292 cells with IFN gamma resulted in a hyperresponsive IL 6 and IL 8 production to TNF alpha and LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Thus , induction of seven chemokines , including T cell activation gene 3 ( TCA 3 ) , monocyte chemoattractant protein 1 , and IFN gamma inducible protein 10 within the CNS during experimental autoimmune encephalomyelitis fails to occur early in the inflammatory process in TNF deficient mice , despite local expression of monokines and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Biopsies from human localized cutaneous lesions ( LCL n = 7 ) or disseminated lesions ( DL n = 8 ) cases were characterized according to cellular infiltration , frequency of cytokine ( IFN gamma , TNF alpha ) or iNOS enzyme producing cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1 alpha , IL 2 , IL 10 , tumor necrosis factor alpha , and IFN gamma were determined in serum and peritoneal fluid . ^^^ IFN gamma , IL 10 , and tumor necrosis factor alpha were detected most frequently . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Proinflammatory and type 1 cytokines including TNF alpha , IL 1beta , IFN gamma , IL 2 , RANTES , and IP 10 were elevated in CNS tissues from mice with PREMO . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our model of immunity to Trichuris has developed from one resolving round IL 4 and IFN gamma to one that also has to accommodate IL 9 , IL 10 , IL 13 , TNF alpha , IL 12 , and IL 18 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In those studies , we demonstrated that the increased CD 57 ( + ) T cell population rapidly produces interferon gamma ( IFN gamma ) and tumor necrosis factor alpha , independent of a second signal requirement , consistent with an increased effector T cell population . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TRAIL induced much stronger cytotoxicity and apoptosis to beta cell lines CM and HP 62 than did FasL , TNF alpha , LTalpha1beta2 , LTalpha2beta1 , LIGHT , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Only the relation between IFN gamma and TNF alpha was significant in Group A . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , we found a diminished IFN gamma , TNF alpha and IL 2 deposition in the transplant area , and a reduced co localization of these cytokines with laminin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A similar change in the rate of oxygen consumption is observed when Caco 2 human enterocyte like cells are incubated in vitro with cytomix , a cocktail of cytokines containing tumor necrosis factor , IL 1beta , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We examined the possible roles of the cytokines tumor necrosis factor alpha ( TNF alpha ) and gamma interferon ( IFN gamma ) in these adverse outcomes . ^^^ Placental production of TNF alpha , but not of IFN gamma , may be implicated in impaired fetal growth in Malawian women . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Nevertheless , there was a progressive diminution of their effector activities as their capacity to produce first IL 2 , then TNF alpha , and finally IFN gamma was lost . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To elucidate the mechanisms underlying long term activation of NK cells during treatment with D Fraction , we examined tumor volume and levels of IFN gamma and TNF alpha in MM 46 bearing C3H / HeN mice to which D Fraction was administered for 19 d . ^^^ D Fraction markedly suppressed tumor growth , corresponding with increases in TNF alpha and IFN gamma released from spleen cells and a significant increase in TNF alpha expressed in NK cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Proinflammatory cytokines ( interleukin 1beta [ IL 1beta ] , tumor necrosis factor alpha [ TNF alpha ] , and gamma interferon [ IFN gamma ] ) initiate a variety of signal cascades in pancreatic beta cells that affect the expression level of genes involved in both the destruction and the protection of the beta cell . ^^^ Cytokine induced ( IL 1beta or cytokine mix consisting of IL 1beta + TNF alpha + IFN gamma ) activation of NF kappaB in RINm5F cells was reduced by > 80 % through overexpression of MnSOD . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We find that IL 15 increases 2 to 5 fold both the activation and secretion of the effector cytokines IFN gamma and tumor necrosis factor ( TNF ) alpha by anti CD 3 stimulated purified CD 4 ( + ) and CD 8 ( + ) T cells and peripheral blood mononuclear cells from healthy and HIV infected individuals . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Alpha interferon ( IFN alpha ) , IFN gamma , and ribavirin exhibited antiviral activity , while double stranded RNA ( dsRNA ) and tumor necrosis factor alpha did not . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Frequencies of CMV specific CD 4 ( + ) T cells detected by ICC , both IFN gamma and TNF alpha , were higher in 2 children with symptomatic congenital CMV infection than those in 2 children with asymptomatic congenital infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We examined the time course for mechanical lung function , infiltration of inflammatory cells into the lung , and the expression of three inflammatory cytokines , tumor necrosis factor alpha ( TNF alpha ) , macrophage inflammatory protein 2 ( MIP 2 ) and interferon gamma ( IFN gamma ) . ^^^ The differences in the time course and primary site of TNF alpha , MIP 2 , and IFN gamma expression ; and the differences in the temporal relationship between their expression and infiltration of inflammatory cells may have accounted for the differences in lung function between paraquat treated and ACM smoke exposed animals . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Nevertheless , this mixed response was smaller than in active lesions , with reduced but present numbers of cells expressing TNF alpha , IFN gamma and IL 4 and sustained numbers of cells expressing IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Other cytokines , e . g . interleukin ( IL ) 2 , IL 4 , IL 6 , IFN alpha / beta and tumor necrosis factor alpha ( TNF alpha ) , either alone or in combination with IFN gamma , did not greatly modulate NO levels produced by resident peritoneal exudate cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : PD analyses suggested that in NHS samples containing CsA+SRL ( n=5 ) , ( 1 ) PMA Ionomycin stimulated T cell expression of intracellular IL 2 , TNF alpha , and IFN gamma was inhibited by CsA , and minimally by SRL , and ( 2 ) the two agents inhibited pokeweed mitogen ( PWM ) stimulated B cell expression of CD 54 and CD 95 , but not CD 86 ( ICAM 1 , Fas antigen , and B7 . 2 ) , synergistically . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
FL plus IL 15 driven ACF / CD56 ( + ) cells progressively expressed a mature NK functional program lysing both NK and lymphokine activate killer ( LAK ) sensitive tumor targets and producing high levels of interferon gamma ( IFN gamma ) , granulocyte macrophage colony stimulating factor , tumor necrosis factor alpha , and IL 10 upon stimulation with IL 12 and IL 18 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These effects were partially opposed by IFN gamma and TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MHC class 2 transcript levels induced by IFN gamma alone or in combination with TNF alpha were also clearly diminished after parasitic infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation with TNF alpha , IFN gamma , LPS or combination of TNF alpha and IFN gamma significantly upregulated CD 40 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
DNA was extracted from leukocytes and assayed for TNF alpha , IFN gamma , IL 6 , IL 10 and TGF beta 1 genotypes using polymerase chain reaction sequence specific primer technology . ^^^ IFN gamma genotype was directly related with the frequency of subjects having at least a four fold increase in RSV specific serum IgG and TNF alpha genotype was inversely associated with the frequency of subjects having at least a twofold increase in RSV specific nasal IgA . ^^^ Additionally , IL 6 genotype was predictive of certain measures of illness expression , while IFN gamma genotype predicted IL 1 protein levels , and TNF alpha genotype predicted IL 6 and IL 8 protein levels in nasal lavage fluids . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The multi probe RPA simultaneously quantitated mRNA levels of equine IL 1alpha , IL 1beta , IL 6 , IL 8 , IL 10 , IL 12 p 35 , IL 12 p 40 , IFN gamma , TGF 1beta and TNF alpha in EPBMC and EMDM with coefficients of variation as low as 0 . 03 0 . 08 ( 3 8 % ) when normalized to beta actin expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma also increased LPS induced tumor necrosis factor ( TNF ) alpha in BAL fluid . ^^^ LPS induced TNF alpha and CINC mRNA expression in alveolar macrophages was increased by IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In combination with the RT PCR method , this model was also used to study the dynamics of cytokine mRNA activity for IFN alpha , IFN gamma , IL 1 beta , IL 2 , IL 4 , IL 6 , IL 8 , BL 10 , IL 12 , IL 18 , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma and TNF alpha production in gamma irradiated blood units by mononuclear cells and GVHD prevention . ^^^ IFN gamma , a Th 1 cytokine , and TNF alpha , a pro inflammatory one , had a central role in the stimulation of cellular and inflammatory reactions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Effect of IFN gamma and TNF alpha on hepatitis B virus posttranscriptional regulatory elements ] . ^^^ OBJECTIVE : To explore the effect of IFN gamma and TNF alpha on hepatitis B virus posttranscriptional regulatory elements . ^^^ After incubation with IFN gamma , TNF alpha and IFN gamma + TNF alpha , the normalized CAT activity from recombinant pDM 138 produced in transfected cells were 324 + / 57 , 396 + / 82 and 175 + / 36 pg / ml , respectively ( t = 5 . 19 , 4 . 39 , 6 . 68 , P < 0 . 01 ) . ^^^ The effect of IFN gamma and TNF alpha were dose dependent over a range of 0 to 1 , 000 U / ml . ^^^ CONCLUSION : The expression of HBV gene may be regulated by IFN gamma and TNF alpha through inhibiting the function of HPRE . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
BCR / ABL also decreased the natural cytotoxicity of NK 92 cells against K 562 targets , without affecting IL 2 , interferon gamma ( IFN gamma ) , or tumor necrosis factor alpha ( TNF alpha ) production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The levels of LTB 4 , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) were all significantly lower in the treated group ( p < 0 . 05 , Student ' s t test ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of cytokines / chemokines on HIV 1 replication in cells of myeloid lineage can be inhibitory ( IFN alpha , IFN beta , IFN gamma , GM CSF , IL 10 , IL 13 and IL 16 and beta chemokines ) , stimulatory ( M CSF , TNF alpha , TNF beta , IL 1 , IL 6 ) or bifunction al , that is both inhibitory and stimulatory ( IL 4 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The development of granulomatous inflammation in patients with CBD is associated with the production of numerous inflammatory cytokines , including IFN gamma , IL 2 , and TNF alpha ( see Fig . 1 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pretreatment of HDMEC with TNF alpha and IFN gamma resulted in an enhanced attachment of both DC precursors and mature DC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There was no statistically significant difference in the expression of IFN gamma and TNF alpha mRNA between the IRF 1 knockout and wild type mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
LPMC from involved ileal , Crohn ' s disease produced , in both non stimulated and stimulated conditions , more IFN gamma and IL 12p70 than LPMC from non involved tissue or from control patients . rhIFN gamma significantly enhanced TNF production in both controls and in ileal Crohn ' s disease patients , while rhIL 12 enhanced IFN gamma but not TNF production . ^^^ LP T cell dependent activation of monocytes was then studied by co culture of anti CD3 / CD80 stimulated LPMC with fresh monocytes , which resulted in high IL 12 , IFN gamma , TNF and IL 10 production . ^^^ However , no effect of anti IL 12 or anti IFN gamma on production of TNF , a cytokine with an important pathogenic role in Crohn ' s disease , could be found . ^^^ Therapies aiming at neutralisation of IFN gamma or IL 12 are therefore unlikely to replace anti TNF , but they might provide an additive or synergistic effect . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
C1q bearing immune complexes , but not unopsonized complexes , elicited both TNF alpha and IFN gamma secretion from human T cells . ^^^ Reverse transcriptase polymerase chain reaction ( RT PCR ) of immune complex stimulated cells revealed accumulation of both TNF alpha and IFN gamma mRNA within 2 h post stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Exposure of islet cells to proinflammatory cytokines IL 1beta , TNF alpha , and IFN gamma , or to the mitochondrial uncoupler FCCP resulted in disruption of the mitochondrial membrane potential ( ( m ) ) and beta cell death . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) further increased the CD 95 expression induced by positive cytokines . ^^^ Viable cells and CFU C numbers were 31 . 9 + / 11 . 2 and 43 + / 2 . 0 respectively , the rate of apoptosis was 42 . 9 + / 12 . 4 in the presence of anti CD 95 McAb and TNF alpha or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The BALC ' s were analyzed for the cytokines TNF alpha , IFN gamma , IL 8 , IL 10 , and IL 12 ( p 40 ) by real time TaqMan polymerase chain reaction in 2 , 4 , and 6 week old piglets , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , treatment of B . burgdorferi vaccinated , challenged IFN gamma ( 0 ) mice with recombinant TNF alpha ( rTNF alpha ) inhibited the development of arthritis , including swelling of the hind paws . ^^^ Moreover , treatment of vaccinated , challenged IFN gamma ( 0 ) mice with anti TNF alpha inhibited fourfold the production of an antibody that kills B . burgdorferi , while treatment of vaccinated , challenged IFN gamma ( 0 ) mice with rTNF alpha slightly elevated the level of the borreliacidal antibody . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
INTERVENTION ( S ) : Endometrial stromal cells were incubated for 24 hours with IL 1alpha , IL 1beta , TNF alpha , TNF beta , IFN gamma , and LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In a separate experiment , 30 mice were fed a CLA supplemented diet or a corn oil supplemented diet for 6 weeks and peritoneal resident macrophages were obtained for measuring TNF alpha and nitric oxide production after in vitro exposure to interferon gamma ( IFN gamma ) and / or LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Production of tumour necrosis factor alpha ( TNF alpha ) , IL 1 beta , interferon gamma ( IFN gamma ) , and IL 4 was measured by enzyme linked immunosorbent assay ( ELISA ) and by single cell FACS analysis . ^^^ IL 7 stimulated TNFalpha production by SFMC and very potently stimulated IFN gamma and TNF alpha production by SF CD4+ T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We studied the effects of tryptophan ( TRP ) availability on the synthesis and release of nitric oxide ( NO ) and tumor necrosis factor alpha ( TNF alpha ) in interferon gamma ( IFN gamma ) activated murine macrophages of the BAC1 . 2F5 cell line . ^^^ IFN gamma ( 100 U / ml ) not only increased the synthesis and release of NO and TNF alpha from these cells but also induced indoleamine 2 , 3 dioxygenase , the rate limiting enzyme of TRP catabolism . ^^^ In TRP ' ' starved ' ' cultures , in fact , the IFN gamma mediated NO synthase induction was significantly reduced , and the increased TNF alpha synthesis and release were not affected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mucosal mRNA levels for IL 1beta , IL 2 , IL 4 , IL 5 , IL 8 , IL 10 , IL 12p35 , IL 12p40 , IL 15 , IFN gamma and TNF alpha were simultaneously analyzed by real time quantitative polymerase chain reaction . ^^^ In patients with active ulcerative colitis , IL 1beta , IL 4 , IL 5 , IL 8 , IL 12p40 , IFN gamma and TNF alpha mRNA levels were significantly higher than those in controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study we investigated the expression of intercellular cell adhesion molecule 1 ( ICAM 1 ) in transformed endothelial cell line ECV 304 cells as influenced by lovastatin , tumor necrosis factor alpha ( TNF alpha ) and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of THI 52 ( 1 naphthylethyl 6 , 7 dihydroxy 1 , 2 , 3 , 4 tetrahydroisoquinoline ) on ( a ) inducible nitric oxide synthase ( iNOS ) and tumor necrosis factor alpha ( TNF alpha ) expression in RAW 264 . 7 cells stimulated by lipopolysaccharide ( LPS ) / interferon gamma ( IFN gamma ) , ( b ) plasma nitrate concentration as well as iNOS protein expression ( lung ) in vivo in LPS treated rats , and ( c ) the restoration of vascular contractility to vasoconstrictor agents in LPS treated vessels in vitro were investigated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Relevance of the selected cytokine release ( TNF alpha , IL 6 , IFN gamma , and IFN alpha ) to the exacerbation of bronchial asthma from airway mycotic infections . ^^^ The BAL procedure and measurement of the levels of tumor necrosis factor alpha ( TNF alpha ) , interleukin 6 ( 2 6 ) , interferon gamma ( IFN gamma ) , and interferon alpha ( IFN alpha ) by specific bioassays were performed twice : before antimycotic treatment and after 3 weeks of therapy with 8 mg of nebulized fluoconazole and 400 mg of oral ketoconazole per day . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Muscle cytokine mRNA content was detected preexercise for seven cytokines in this order ( highest to lowest ) : IL 15 , TNF alpha , IL 8 , IL 1beta , IL 12p35 , IL 6 , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma down regulated the GCP 2 production in fibroblasts induced by IL 1beta , TNF alpha , LPS , or dsRNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum IL 2 , IFN gamma , and TNF alpha levels were increased in most patients in the experimental group , while in the control group , there were no differences of Ab 1 ' and cytokine level between pretherapy and posttherapy . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In both macrovascular ( HUVEC ) and microvascular ( HMVEC ) endothelial cells , fever range hyperthermia ( 40 degrees C for 6 12 h ) did not affect expression of adhesion molecules ( ICAM 1 , E selectin , VCAM 1 , P selectin , PECAM 1 , PNAd , MAdCAM 1 ) , cytokine release ( IL 1beta , TNF alpha , IFN gamma , IL 6 , IL 11 , IL 12 , IL 13 ) , or chemokine secretion ( IL 8 , RANTES , MCP 1 , MIP 1beta , MIG ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Modulation of cdk 2 , cyclin D 1 , p16INK4a , p21WAF and p27Kip1 expression in endothelial cells by TNF / IFN gamma . ^^^ BACKGROUND : Regional administration of high doses of tumor necrosis factor ( TNF ) and interferon gamma ( IFN gamma ) to metastatic melanoma patients causes selective disruption of the tumor vasculature . ^^^ MATERIALS AND METHODS : TNF and IFN gamma treated HUVEC were analyzed for cell cycle progression and for protein expression by flow cytometry and Western blotting , respectively . p16INK4a was overexpressed by transient transfection , and HUVEC adhesion was tested in short term adhesion assays . ^^^ RESULTS : TNF and IFN gamma synergistically induced a G 1 arrest associated with reduced levels of cyclin D 1 and cdk 2 , and increased expression of the cdk inhibitors p16INK4a , p21WAF and p27Kip1 . p16INK4a overexpression , however , had no effect on alpha 5 beta 3 mediated adhesion . ^^^ CONCLUSION : These results implicate the down regulation of cyclin D 1 and cdk 2 , and up regulation of p16INK4a , p21WAF and p27Kip1 in the suppression of endothelial cell proliferation induced by TNF / IFN gamma and demonstrate that increased p16INK4a levels are not sufficient to suppress alpha 5 beta 3 mediated endothelial cell adhesion . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : OK PSA markedly increased the amounts of IFN gamma , tumor necrosis factor ( TNF ) alpha , interleukin ( IL ) 2 , IL 12 and IL 18 that are generally called `` Th 1 type cytokines ' ' in the sera derived from tumor bearing nude mice , and also accelerated the killing activities of tumor infiltrating lymphocytes as well as of draining lymph node cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment with EE significantly decreased the secretion of proinflammatory cytokines ( IFN gamma , TNF alpha , and IL 6 ) by activated T cells as well as the expression of a key matrix metalloproteinase , disease mediating chemokines / receptors , and IgG2a levels , but increased the expression of TGF beta 3 in the CNS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Reverse transcription PCR ( RT PCR ) , performed 5 days p . i . , showed that inducible nitric oxide synthase , tumor necrosis factor alpha ( TNF alpha ) , interleukin 12 p 40 ( IL 12p40 ) , gamma interferon ( IFN gamma ) , and IL 10 mRNAs were similarly induced in spleens of the two strains . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Elevated levels of mRNA for interleukin 12p40 ( IL 12p40 ) , gamma interferon ( IFN gamma ) , tumor necrosis factor alpha , and inducible nitric oxide synthase ( iNOS ) were associated with protection in immunized challenged ( I / C ) mice , but Th 2 cytokine ( IL 4 , IL 5 , IL 10 , and IL 13 ) and chemokine ( KC , MIP 2 , and MCP 1 ) expression was not associated with protection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Among the cytokines elicited by the immune response to Toxoplasma , tumor necrosis factor alpha ( TNF alpha ) acts synergistically with gamma interferon ( IFN gamma ) and plays a major role in host cell resistance . ^^^ We have previously reported that TNF alpha production induced by IFN gamma / LPS decreases after T . gondii infection of human myelomonocytic THP 1 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 4 downregulated IFN gamma and TNF alpha secretion in IL 2 and IL 15 treated NK cells . ^^^ The property of p 17 to increase the production of TNF alpha and IFN gamma might be a mechanism used by HIV 1 to modulate the immune system to support its replication and spreading . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
LC 46 , an HBx 52 60 specific clone , is CD62L ( ) CD 69 ( + ) CD45RO ( + ) CD45RA ( ) CD 25 ( dim ) and is stained by IFN gamma ( approximately 92 % ) , IL 2 ( 30 % ) , and TNF alpha ( 56 % ) , but not by IL 5 , IL 10 , IL 12 , or TNF beta , indicating that the cells are fully activated T cytotoxic 1 type cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha plus IFN gamma induce connexin 43 expression and formation of gap junctions between human monocytes / macrophages that enhance physiological responses . ^^^ In this work , the effects of bacterial LPS , TNF alpha , and IFN gamma on gap junctional communication ( dye coupling ) and on the expression of connexin 43 ( immunofluorescence , immunoblotting , and RT PCR ) in monocytes / macrophages were studied . ^^^ Freshly isolated human monocytes plated at high density and treated either with LPS plus IFN gamma or TNF alpha plus IFN gamma became transiently dye coupled ( Lucifer yellow ) within 24 h . ^^^ Cells treated with LPS , TNF alpha , or IFN gamma alone remained dye uncoupled . ^^^ Abs against TNF alpha significantly reduced the LPS plus IFN gamma induced increase in dye coupling . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mononuclear cells expressed IFN gamma in the superficial lamina propria and TNF alpha adjacent to basal keratinocytes . ^^^ TNF alpha stimulation down regulated IFN gamma secretion and up regulated TNF alpha secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Multi probe RNase protection assay showed the significantly decreased mRNA levels of apoptosis related genes and various cytokines genes like IFN gamma , RANTES , TNF alpha , TNFR p 75 , IL 1beta in HPR group . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This review summarizes recent results investigating the role of certain cytokine gene polymorphisms , including those of TNF alpha , IFN gamma , IL 6 , IL 10 and IL 1 receptor antagonist ( IL 1Ra ) , in allogeneic stem cell transplantation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cells were cultured in a serum free medium with different cytokine combinations including GM CSF , TNF alpha , Flt 3 , CD40L , IFN gamma , IL 1alpha , IL 6 , PGE 1 , and IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using an HBV transgenic mouse model , our laboratory has previously demonstrated that adoptive transfer of HBV specific cytotoxic T lymphocytes or injection of IL 2 can noncytopathically inhibit HBV gene expression by a posttranscriptional IFN gamma and / or tumor necrosis factor alpha dependent mechanism . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The proportions of PPD activated IFN gamma and TNF alpha producing CD 4 ( + ) cells were low among control subjects ( median , 0 . 33 % and 0 . 78 % , respectively ) . ^^^ By contrast , among patients with TB , strong IFN gamma and TNF alpha responses were demonstrated ( median , 24 . 0 % and 32 . 4 % , respectively ) , regardless of whether the TB was pulmonary or nonpulmonary . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of chemokines and their receptors in human and simian astrocytes : evidence for a central role of TNF alpha and IFN gamma in CXCR 4 and CCR 5 modulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Results showed a significant increase in interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) , nitric oxide ( NO ) and soluble Fas in both infected human and mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 10 , IFN gamma and TNF alpha in acute and chronic human fascioliasis . ^^^ The three cytokines , IL 10 ( Th 2 product ) , IFN gamma ( Th 1 product ) and TNF alpha work in concert . ^^^ During the acute stage , a significant increase was observed in IL 10 , IFN gamma as well as TNF alpha and specific antibody level . ^^^ IFN gamma showed a lower level as compared to the acute stage but TNF alpha was still high . ^^^ IL 10 ( Th 2 cytokine ) which is antagonistic to IFN gamma ( Th 1 product ) and consequently to TNF alpha limits the immunopathology that may be caused by the latter . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma and TNF alpha , the two major cytokines associated with DTH , were efficiently induced by BCG . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
No significant differences between the two groups could be detected when expression of IFN gamma , IL 2 , TNF alpha , IL 1beta or IL 10 mRNA was analysed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Benzylpenicillin differentially conjugates to IFN gamma , TNF alpha , IL 1beta , IL 4 and IL 13 but selectively reduces IFN gamma activity . ^^^ By in vitro bioassay , BP inhibited the ability of IFN gamma but not IL 1beta or TNF alpha to induce CD 54 expression on epithelial cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
AIM : To compare the inhibitory effect of the macrophages activated by IFN gamma combined with TNF alpha against RH strain and Fukaya strain . ^^^ RESULTS : In the macrophages activated by 100 U each of IFN gamma and TNF alpha , the invaded tachyzoites of RH strain were completely killed , while the invaded tachyzoites of Fukaya strain remained slow proliferation with significantly lower levels of NO detected at 24 h post challenge . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Combining TNF alpha with IL 6 and IFN gamma pretreatment resulted in restoration of toxoplasmacidal activity . ^^^ Addition of polyclonal anti TNF alpha antibodies to IL 6 and IFN gamma pretreatment resulted in enhancement in the IL 6 mediated impairment of IFN gamma function . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Th 1 lymphocytes seem to orchestrate the inflammation through the production of pro inflammatory cytokines such as IFN gamma , IL 1beta and tumor necrosis factor ( TNF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expansion of colonic and splenic CD 4 ( + ) T cells was reduced as well as the colonic expression of tumor necrosis factor alpha ( TNF alpha ) , IL 1beta , and interferon gamma ( IFN gamma ) mRNA without affecting the production of transforming growth factor beta ( TGF beta ) , IL 10 , and IL 4 mRNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although inflammatory cytokines , including tumor necrosis factor alpha and IFN gamma , are overly produced in these mice , the NAPDH oxidase activity , which is involved in antimicrobial and innate immune responses , is inhibited . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma , TNF alpha , and IL 1beta mRNAs were markedly reduced , but no difference was observed in the expression of IL 4 , IL 10 , and TGF beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this report , we show that intravenous ( i . v . ) injection into mice of a complex made of the cationic lipid diC 14 amidine and the luciferase reporter plasmid ( pCMV luc ) results in efficient gene expression in several organs but elicits an inflammatory response characterized by a release of tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) into the serum of treated animals . ^^^ This improvement is correlated with the ability of free liposomes to inhibit TNF alpha but not IFN gamma production resulting from complex injection . ^^^ TNF alpha rich serum obtained from mice injected with diC 14 amidine / protamine / pCMV luc complex inhibits luciferase expression in transfected mouse lung endothelial ( MLE ) cells cultured in vitro , whereas IFN gamma has no effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Decreases in plasma TNF alpha level and IFN gamma mRNA level in peripheral blood mononuclear cells ( PBMC ) and an increase in IL 2 mRNA level in PBMC are associated with effective highly active antiretroviral therapy in HIV infected patients . ^^^ We also observed a gradual decrease in the number of proviral DNA copies in PBMC and in immune activation , with lower levels of IFN gamma mRNA in PBMC associated with weaker activation of CD8+ T cells and lower levels of plasma TNF alpha . ^^^ The decrease in TNF alpha and IFN gamma levels and the increase in IL 2 production appear to be correlated with the efficacy of HAART in naive immunocompromised HIV infected individuals . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
High plasma levels of TNF , IFN gamma , and liver transaminases alanine aminotransferase ( ALT ) and aspartate aminotransferase ( AST ) were found in these mice , indicating lethal toxic shock . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumour necrosis factor alphaa ( TNF alpha ) , interleukin 1beta ( IL 1beta ) , interleukin 8 ( IL 8 ) and interferon gamma ( IFN gamma ) in CSF were measured by enzyme linked immunosorbent assay , and IL 6 was measured by bioassay . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although IP 10 mRNA expression was abrogated in TNF alpha ( / ) mice , both CHS development and TNF alpha mRNA expression occurred normally in IFN gamma ( / ) mice , indicating that the induction of IP 10 during CHS was primarily controlled by TNF alpha . ^^^ IL 1 induced tumor necrosis factor alpha elicits inflammatory cell infiltration in the skin by inducing IFN gamma inducible protein 10 in the elicitation phase of the contact hypersensitivity response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Effect of recombinant prothymosin alpha on secretion of IFN gamma , IFN alpha and TNF alpha in vitro ] . ^^^ AIM : To study the effect of prothymosin alpha ( Pro T alpha ) as a fusion protein on secretion of IFN gamma , IFN alpha and TNF alpha in vitro . ^^^ Then IFN gamma , IFN alpha and TNF alpha levels in the supernatant were detected by ELISA . ^^^ CONCLUSION : In vitro , Pro T alpha could increase the secretion of IFN gamma , IFN alpha and TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We failed to detect any effects on ex vivo MOG peptide induced IFN gamma , TNF alpha , IL 6 , IL 4 , IL 10 , and brain derived neurotropic factor expression in splenocytes or CNS derived lymphocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The inflammatory cytokines TNF alpha , IFN beta , and IFN gamma induced HLA B strongly , but HLA A and C weakly in both cell types . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF plays an essential role in tumor regression after adoptive transfer of perforin / IFN gamma double knockout effector T cells . ^^^ This study identifies perforin , IFN gamma , and TNF as a critical triad of effector molecules that characterize therapeutic antitumor T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 18 is an important cytokine in autoimmune and inflammatory diseases through the induction of IFN gamma , TNF alpha , and IL 1 . ^^^ The production of IFN gamma , TNF alpha , and IL 1beta in cultured spleen cells was reduced by in vivo treatment with low dose , but not high dose , mIL 18BP . ^^^ The steady state mRNA levels of IFN gamma , TNF alpha , and IL 1beta in isolated joints were all decreased in mice treated with both doses of mIL 18BP . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Significantly greater concentrations of TNF alpha ( mean , 1 , 346 . 4 pg / mL vs 61 . 2 pg / mL ; p < 0 . 05 ) and IFN gamma ( mean , 540 . 9 pg / mL vs 26 . 7 pg / mL ; p < 0 . 05 ) were seen in the serum ( n = 4 ) compared with control subjects . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This effect was associated with an increase in the production of IL 12 , IFN alpha , IL 18 , TNF alpha , IL 10 , and IL 15 by CD 14 ( + ) cells , as well as an increase in the proportions of IFN gamma producing CD 3 ( ) CD 16 ( + ) ( natural killer ) cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Rifaximin administration ( 30 or 50 mg / kg / day ) increased survival rates of colitic mice and reduced colitis severity as demonstrated by improvement of wasting syndrome , histologic scores , decrease in colon IL 2 , IL 12 , IFN gamma and TNF alpha ( protein and mRNA ) levels , and diminished colon MPO activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
For pathophysiological analyses , we measured pro and anti inflammatory cytokines ( IL 1 , IL 6 , TNF alpha , IFN gamma , IL 4 , IL 10 , IL 12 ) , Ag specific T cell proliferation and IL 2 production , serum IgG 1 and IgG 2 levels of both auto and heteroantibodies , and soluble CD 44 . ^^^ High levels of TNF alpha , IFN gamma , IL 2 , and Ab production were detected in F ( 2 ) hybrids with CIA , whereas T cell proliferation , IL 2 and IFN gamma production , and a shift to IgG2a isotype were more characteristic of PGIA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both CCR 7 ( int ) and CCR 7 ( ) Ag specific CD 8 ( + ) T cells produced IFN gamma and TNF alpha following short term peptide stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) and IFN gamma cytokine secretion in the 10 , 000 IU group at month 5 showed a significant decrease that corresponded with the effect of ingested IFN alpha on decreasing gadolinium enhancements . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The synovial mRNA content of tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 ( IL 1 ) , interleukin 6 ( IL 6 ) , interleukin 12 ( IL 12 ) , transforming growth factor beta ( TGF beta ) , and interferon gamma ( IFN gamma ) was determined by RT PCR ( reverse transcription polymerase chain reaction ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
D GalN also enhanced TNF alpha , IL 1alpha , IL 6 and IFN gamma concentrations in blood . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Within this compartment , the TNF and IFN gamma responsive acid sphingomyelinase ( ASMase ) generates the signaling molecule ceramide , resulting in the activation of proteases like cathepsin D . ^^^ To investigate the possible role of ASMase as a mediator of the antibacterial effects of TNF and IFN gamma , ASMase ( / ) mice were infected with Listeria monocytogenes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha matured DC phenotypically , but additional interferon gamma ( IFN gamma ) treatment was necessary to achieve functional maturation , the production of significant amounts of IL 12 . ^^^ Our results suggest that after allowing 4 h of tumor lysate uptake by immature DC , further treatment with TNF alpha and IFN gamma for 24 h provides the optimal conditions to obtain functional TAA loaded DC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
By day 5 , mRNA levels for the pro inflammatory cytokines ( IL 1beta , TNF alpha , IFN gamma ) , chemokines ( MCP 1 , IP 10 ) , and an adhesion molecule ( ICAM 1 ) had returned to baseline in saline injected mice and to near baseline levels in helper virus free amplicon groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Compared with controls , asymptomatic patients had increased production of IL 1beta and IL 12 ( both P < 0 . 05 ) , unchanged production of TNF alpha , IFN gamma and IL 1ra and notably reduced production of IL 10 , IL 2 and sIL 2 ralpha ( all P < 0 . 05 ) . ^^^ HIV progression led to a progressive decline in whole blood culture production of TNF alpha , IFN gamma , IL 1ra , IL 1beta , IL 12 , IL 10 and IL 2 ( all P < 0 . 0001 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It has been shown that interferon gamma ( IFN gamma ) plays a role in the regulation of interleukin 8 ( IL 8 ) , nitric oxide ( NO ) , and tumor necrosis factor alpha ( TNF alpha ) secretion by macrophages stimulated with lignin derivatives , such as EP 3 , and lipopolysaccharides ( LPS ) [ Cytokine 11 ( 1999 ) 571 ] . ^^^ To examine the mechanism by which IFN gamma affects secretion of these factors , EP 3 or LPS stimulated macrophages were treated with different concentrations of IFN gamma , and mRNA levels of IL 8 , nitric oxide synthase ( NOS ) and TNF alpha were determined by Northern blot analysis and reverse transcription polymerase chain reaction ( RT PCR ) . ^^^ IFN gamma , which increased the amount of TNF alpha mRNA in unstimulated macrophages , showed no significant effect on the high level of TNF alpha mRNA in stimulated macrophages . ^^^ Effects of IFN gamma on TNF alpha secretion by stimulated macrophages may be mediated by a different mechanism . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 18 plays an important role in inducing Th 1 response because it is a potent inductor of synthesis of IFN gamma , TNF and other mediators . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Histopathological modulation was accompanied by a modified cytokine pattern from popliteal lymph node cells , demonstrated by a sustained higher frequency of interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) producing cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Indeed , they kill mycobacteria infected macrophages and , upon recognition of mycobacterial Ag , release TNF alpha and IFN gamma , which are also up regulators of HIV expression . ^^^ Both effects were mainly mediated by release of TNF alpha and IFN gamma from activated gamma delta lymphocytes , although Fas FasL interaction also contributed to U 1 apoptosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interestingly , low levels of both total and specific immunoglobulin G2a were observed in MIF ( / ) cysticercotic mice despite the high IFN gamma levels ; in addition , peritoneal macrophages obtained from T . crassiceps infected MIF ( / ) mice at different time points failed to respond efficiently to stimulation in vitro with lipopolysaccharide plus IFN gamma and produced significantly lower levels of IL 12 , tumor necrosis factor alpha , and NO compared to those from MIF ( + / + ) mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mesopram , a specific inhibitor of type 4 phosphodiesterase , decreases the synthesis of tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) . ^^^ Post mortem , colon length , histologic score , and expression of TNF alpha and IFN gamma in colons were determined . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin 2 ( IL 2 ) , IL 4 , IL 10 , IFN gamma , TNF alpha , TGF beta 1 and leptin were measured by enzyme immunoassay ( EIA ) ; nitrite by the Griess reaction . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Since certain cytokines may play a role in unexplained recurrent pregnancy loss ( RPL ) and also some cytokine gene polymorphisms may affect the level of cytokine production , the aim of the present study was to investigate the relationship between RPL and polymorphisms of the genes coding for TNF alpha ( 308 G > A ) , IL 10 ( 1082 G > A ) , IL 6 ( 174 G > C ) , and IFN gamma ( +874 A > T ) . ^^^ In addition , we performed a meta analysis encompassing the present results and those from studies on the association of TNF alpha , IL 10 and IFN gamma polymorphisms with RPL published in the literature until December 2001 . ^^^ The results showed : ( 1 ) no evidence of association with IL 6 gene polymorphisms ; ( 2 ) significant associations , revealed by the meta analysis , with the high cytokine production genotypes of IFN gamma ( +874 T / T : odds ratio ( OR ) =1 . 92 , P=0 . 04 ) and IL 10 ( 1082 G / G : OR=1 . 75 , P=0 . 03 ) , and a trend for association with the high TNF alpha production genotypes 308 A / A and A / G ( OR=1 . 61 ; P=0 . 18 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Minimal to modest K ( b ) tetramer binding of mPrP amino acids ( aa ) 2 to 9 , aa 152 to 160 , and aa 232 to 241 was observed , but such tetramer binding lymphocytes as well as CD 4 and CD 8 lymphocytes incubated with the full repertoire of mPrP peptides failed to synthesize intracellular gamma interferon ( IFN gamma ) or tumor necrosis factor alpha ( TNF alpha ) cytokines and were unable to lyse PrP ( / ) embryo fibroblasts or macrophages coated with ( 51 ) Cr labeled mPrP peptide . ^^^ However , these CD 4 and CD 8 T cells are defective in such an effector function ( s ) as IFN gamma and TNF alpha expression or release or lytic activity . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : stimulation with inflammatory cytokines , including interleukin ( IL ) 1 , IL 4 , IL 6 , IL 10 , interferon ( IFN ) alpha , IFN beta , IFN gamma , and tumor necrosis factor ( TNF ) alpha failed to induce TARC mRNA expression in NHEK . ^^^ However , stimulation with IFN gamma and TNF alpha together enhanced expression slightly . ^^^ ELISA analysis failed to detect TARC protein in NHEK culture supernatant , even following stimulation with IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The mechanisms that regulate IL 13 production and / or function have also been investigated , and IL 4 , IL 12 , IL 18 , IFN gamma , IL 10 , TGF beta , TNF alpha , and the IL 4 / IL 13 receptor complex play important roles . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The exposure had little effect on the weights of immune organs and the spleen or the thymus cell population in the dams and offspring , but it enhanced the production of tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) in the serum after Listeria infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Heat killed pathogenic Leptospira interrogans serovar rachmati induced the production of gamma interferon ( IFN gamma ) and the IFN gamma inducing cytokines interleukin 12p40 ( IL 12p40 ) and tumor necrosis factor alpha in human whole blood in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In Th 1 cells , NFATx enhanced the expression of the Th 1 cytokine genes , IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : FACS purified rat beta cells and human islets were cultured with IL 1beta , IFN gamma and / or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin 1alpha ( IL 1alpha ) , IL 1beta , IL 2 , IL 4 , IL 6 , IL 8 , IL 10 , IL 12p35 , IL 12p40 , interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and transforming growth factor beta 1 ( TGF beta 1 ) were cloned and transcribed in vitro to generate antisense probes for ribonuclease protection assays . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Supernatants of peripheral blood mononuclear cells were analyzed for tuberculin purified protein derivative induced production of the type 1 cytokine IFN gamma , tetanus toxoid induced production of the type 2 cytokine IL 5 and the BCG induced production of TNF alpha by a double sandwich ELISA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vivo treatment with anti OX40L MAb decreased CD 4 ( + ) T cell infiltration in the colon and suppressed IFN gamma , IL 2 , and TNF alpha production by lamina propria CD 4 ( + ) T cells . ^^^ The combination with anti TNF alpha MAb further improved the therapeutic effect by abolishing IFN gamma , IL 2 , and TNF alpha production by lamina propria CD 4 ( + ) T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TGF beta / Smad signaling inhibits IFN gamma and TNF alpha induced TARC ( CCL 17 ) production in HaCaT cells . ^^^ BACKGROUND : A Th 2 chemokine , thymus and activation regulated chemokine ( TARC / CCL17 ) , produced by keratinocytes , is implicated in the development of atopic dermatitis by recruiting CLA ( + ) CCR 4 ( + ) lymphocytes into lesional skin and its expression was induced by proinflammatory cytokines such as interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^ METHODS : The effect of TGF beta 1 on mRNA and protein expression of IFN gamma and TNF alpha induced TARC in a human keratinocyte cell line , HaCaT cells , was evaluated by using RT PCR and ELISA . ^^^ RESULTS : TGF beta 1 inhibited mRNA and protein expression of IFN gamma and TNF alpha induced TARC in HaCaT cells . ^^^ In addition , overexpression of Smad 2 or Smad 3 , major signal transducing Smads , was sufficient to inhibite the IFN gamma and TNF alpha induced TARC production in HaCaT cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We analyzed the effect of KE 758 on tumor necrosis factor ( TNF ) alpha and interleukin ( IL ) 1 beta production by a human monocytes cell line ( THP 1 cells ) , stimulated with interferon gamma ( IFN gamma ) plus lipopolysaccharide ( LPS ) . ^^^ THP 1 cells were treated with IFN gamma for 16 h and were then exposed to LPS for an additional 6 h ( for TNF alpha detection ) or 24 h ( for IL 1 beta detection ) . ^^^ KE 758 had no effect on p 75 and p 55 soluble TNF receptor production in IFN gamma and LPS stimulated THP 1 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Enhancement of cytokine levels by LF was observed for IL 12 at day 5 and IFN gamma at day 9 , but not for TNF alpha or IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Results suggested the implication of IFN gamma in FB ( 1 ) induced hepatotoxicity , which can be explained by a lack of TNF alpha and IL 12 amplification in the liver of the GKO mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The most prominent responses are transient leukopenia , thrombocytopenia , serum transaminase elevations , and elevations of proinflammatory cytokines such as interferon gamma ( IFN gamma ) , interleukin 12 ( IL 12 ) , and tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It is our thesis that the polymerase chain reaction ( PCR ) assay for HSV DNA detection in lesional skin and staining with antibodies to IFN gamma and TNF alpha , are important criteria for the diagnosis of skin eruptions and improved patient management . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we examined the direct effects of several cytokines tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , interleukin 2 and 4 ( IL 2 and 4 ) on TJ in immortalized mouse cholangiocytes . ^^^ RESULTS : TNF alpha and IFN gamma , but neither IL 2 nor IL 4 , significantly decreased TER ( P < 0 . 005 ) . 3H inulin flux studies confirmed IFN alpha induced increases in paracellular permeability of cholangiocytes ( P < 0 . 001 ) . ^^^ In immunostaining and immunoblotting studies , TJ associated proteins were well preserved in TNF alpha or IFN gamma treated cells . ^^^ Ursodeoxycholic acid has been found to have no inhibitory effect on increased paracellular permeability induced by TNF alpha or IFN gamma . ^^^ CONCLUSION : These findings show that TNF alpha and IFN gamma disrupt barrier function of TJ in cholangiocytes without major structural changes to TJ and suggest that disruption of TJ function and subsequent leakage of the bile constituents may influence the aggravation of cholestasis in PBC . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Flow cytometry for the detection of intracellular interferon gamma ( IFN gamma ) , interleukin ( IL ) 2 , IL 4 , IL 6 , IL 10 , IL 13 and tumour necrosis factor ( TNF ) alpha expression in peripheral blood mononuclear cells stimulated with phorbol 12 myristate 13 acetate and ionomycin was performed , followed by treatment with liposomal amphotericin B . ^^^ NK cells and gammadelta+ T cells , thought to be involved in innate host defences against Leishmania , expressed IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Differential expression of activating ( IL 2 , IFN gamma and TNF alpha ) as well as deactivating ( IL 4 and TGF beta ) cytokines was demonstrable in the spleen and liver of animals during the course of infection . ^^^ Further , the synthesis of inducible nitric oxide synthase ( iNOS ) enzyme increased considerably in the liver as well as in the spleen of 4 month infected animal with parallel increase in the transcripts of the iNOS activating cytokines IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
They had increased expression of MHC and costimulatory molecules and expressed elevated levels of B 220 and DEC 205 but had minimal CD 4 staining . mFlt3L DC also had a markedly altered cytokine profile , including lowered secretion of IL 6 , IL 10 , IFN gamma , and TNF alpha , but had a slightly increased capacity to stimulate T cells in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma induces high mobility group box 1 protein release partly through a TNF dependent mechanism . ^^^ IFN gamma , an immunoregulatory cytokine known to mediate the innate immune response , dose dependently induced the release of HMGB 1 , TNF , and NO , but not other cytokines such as IL 1alpha , IL 1beta , or IL 6 . ^^^ Pharmacological suppression of TNF activity with neutralizing Abs , or genetic disruption of TNF expression ( TNF knockout ) partially ( 50 60 % ) inhibited IFN gamma mediated HMGB 1 release . ^^^ These data suggest that IFN gamma plays an important role in the regulation of HMGB 1 release through a TNF and Janus kinase 2 dependent mechanism . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The protective effect of the combination therapy is also supported by light microscopic studies , immunostaining of renal tissue sections for pro inflammatory cytokines ( TNF alpha and IFN gamma ) , iNOS , and apoptosis by TUNEL assay . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Taken together , our results suggest that for effective killing of P . brasiliensis by monocytes , an initial activation signal induced by IFN gamma is necessary to stimulate the cells to produce TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : The intranasal application of microgram doses of chitin microparticles is an effective treatment for reducing serum IgE and peripheral blood eosinophilia , airway hyper responsiveness and lung inflammation in both allergy models results in elevation in Th 1 cytokines IL 12 , IFN gamma and TNF alpha and reduction in IL 4 production during allergen challenge . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When eruptions recurred , relative levels of transcripts for IL 8 , MIP 1alpha , RANTES ( regulated upon activation normal T cell expressed and secreted ) , IL 2 , IFN gamma and TNF alpha were very much higher than those prior to the recurrence , while relative levels of mRNAs for IL 4 and IL 5 did not increase . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Neither TNF alpha , IFN gamma , IL 4 , nor IL 13 alone induced the release of TARC from or affected the amount of TARC mRNA in corneal , dermal , or lung fibroblasts . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The production of TNF alpha and IFN gamma was similar in the LTA and control groups , and both were higher than the levels in the RP group . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We tested for both binding and neutralizing autoantibodies to a range of human cytokines , including interleukin 1alpha ( IL 1alpha ) , IL 1beta , IL 2 , IL 4 , IL 6 , IL 8 , IL 10 , IL 12 , IL 18 , interferon alpha 2 ( IFN alpha 2 ) , IFN omega , IFN beta , IFN gamma , tumour necrosis factor alpha ( TNF alpha ) , transforming growth factor beta 1 ( TGF beta 1 ) and granulocyte macrophage colony stimulating factor ( GM CSF ) , in plasmas or sera . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NO production by interferon gamma ( IFN gamma ) and IFN gamma plus Mycobacterium avium stimulated AMs was mediated through tumor necrosis factor alpha ( TNF alpha ) production , as addition of neutralizing anti TNF alpha antibodies during AMs stimulation resulted in reduced NO production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , in cultured luteal cells , reactivity against Fas ligand ( FasL ) was caused by pretreatment with TNF alpha and IFN gamma and apoptosis was induced by FasL treatment . ^^^ These findings support the hypothesis that macrophages initiate T lymphocyte aggregation at the early stage of luteal regression , and then T lymphocytes induce apoptosis on luteal cells , which in turn develop sensitivity against FasL by TNF alpha and IFN gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The combinations of cytokines such as SCF + FL could up regulate the expression of CD 95 in vitro culture and tumor necrosis factor alpha ( TNF alpha ) and interon gamma ( IFN gamma ) further increased the CD 95 expression induced by positive cytokines . ^^^ A decrease of viable cells , CFU C and LTIC numbers were observed in the presence of anti CD 95 McAbs and TNF alpha or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , tumor necrosis factor alpha ( TNF alpha ) is one of the central regulatory cytokines in macrophage antimicrobial activity and synergizes with IFN gamma in the induction of NO synthesis . ^^^ In the presence of IFN gamma , both FPP fractions stimulated TNF alpha secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Proportions ( % ) of lymphocytes containing IFN gamma , TNF alpha , IL 4 and IL 10 and the Th1 / Th2 ratios of IFN gamma / IL 4 , IFN gamma / IL 10 , TNF alpha / IL 4 and TNF alpha / IL 10 in CD3+ , CD3+ / CD8 ( T helper ) and CD3+ / CD8+ ( T suppressor ) cells were measured by 4 colour flow cytometry . ^^^ RESULTS : RSA women demonstrated significantly higher Th1 / Th2 ratios of IFN gamma / IL 4 ( P < 0 . 01 ) , TNF alpha / IL 4 and TNF alpha / IL 10 ( P < 0 . 05 each ) in CD3+ / CD8 T helper cells than those of controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Compared with RSV alone , tumor necrosis factor alpha ( TNF alpha ) protein was reduced in the bronchoalveolar lavage fluid ( BALF ) on days 1 and 2 of infection ; there was also a reduction in BALF lymphocyte numbers on day 4 , which correlated with reductions in both IFN gamma inducible protein ( IP 10 ) , lymphotactin , and IFN gamma mRNAs in the lungs of RSV + CB mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TaqMan real time PCR was used to study the transcriptional activity of the bovine IL 2 , IL 6 , IL 12p40 , IFN gamma , TNF alpha and granulocyte monocyte colony stimulating factor of whole milk cells in bovine mammary gland experimentally infected with Staphylococcus aureus . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 2 , IL 4 , IL 5 , IFN gamma , TNF alpha and IL 10 mRNA were measured by RT PCR . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Functional abolishment of any one of these cytokines ( IL 2 , IL 6 , IL 12 , IL 18 , GMCSF , TNF alpha , or IFN alpha , except IL 10 ) by neutralizing antibodies leads to reduced IFN gamma production ( 19 82 % inhibition in mouse and 44 77 % inhibition in human systems , respectively ) . ^^^ In mice cytokines IL 2 , IL 12 , IL 18 , and GMCSF are observed to synergize with BCG for IFN gamma production , whereas in human cytokines IL 2 , IL 12 , TNF alpha , and IFN alpha exhibit similar synergistic effects . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
At week 14 postinfection ( mid acute phase ) , mRNAs for leukocyte markers ( CD 3 , CD 4 , CD 8 ) , type 1 cytokines and related transcription factors ( IFN gamma , TNF alpha , STAT 4 , T bet ) , and IL 10 were increased in livers from resolving infections , but mRNAs of other type 1 ( IL 2 ) and type 2 ( IL 4 , STAT 6 , and GATA 3 markers remained at baseline levels . ^^^ Increased coexpression of IFN gamma and TNF alpha mRNAs correlated in most cases with lower levels of intrahepatic WHV covalently closed circular DNA ( cccDNA ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Anti IFN gamma has been tested in several T helper cell ( Th 1 ) ADs , including rheumatoid arthritis ( RA ) , multiple sclerosis ( MS ) , corneal transplant rejection , uveitis , Type 1 diabetes , schizophrenia ( anti IFN gamma and anti TNF alpha ) , and various autoimmune skin diseases ( alopecia areata , psoriasis vulgaris , vitiligo , pemphigus vulgaris and epidermolysis bullosa ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Regulation of Staphylococcus epidermidis induced IFN gamma in whole human blood : the role of endogenous IL 18 , IL 12 , IL 1 , and TNF . ^^^ Interleukin 12 ( IL 12 ) and IL 18 act synergistically to stimulate interferon gamma ( IFN gamma ) production ; moreover , IL 1 and tumor necrosis factor ( TNF ) may also augment IFN gamma synthesis . ^^^ We have investigated the relative contributions of these cytokines in the production of IFN gamma and TNF by the Gram positive bacterium Staphylococcus epidermidis , using the specific cytokine inhibitors IL 18 binding protein ( IL 18BP ) , IL 1 receptor antagonist ( IL 1Ra ) , anti IL 12 antibodies ( anti IL 12 Ab ) , and TNF binding protein . ^^^ Blocking endogenous IL 12 and TNF reduced IFN gamma production by 69 and 36 % . ^^^ In conclusion , S . epidermidis stimulates IFN gamma which is IL 18 , IL 12 and TNF dependent , but IL 1 independent . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both IL 4 and IL 13 inhibit the TNF alpha and IFN gamma enhanced MDC production in a human keratinocyte cell line , HaCaT cells . ^^^ RESULTS : MDC mRNA was weakly expressed in HaCaT cells , and upon stimulation with TNF alpha or IFN gamma , MDC expression was strongly upregulated . ^^^ The supernatant MDC levels when stimulated with TNF alpha or IFN gamma were significantly higher than those without stimulation , and were synergistically increased when stimulated with a combination of TNF alpha and IFN gamma . ^^^ Both interleukin 4 ( IL 4 ) and IL 13 inhibited TNF alpha and IFN gamma enhanced MDC production in HaCaT cells in a dose dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When analysis of the most important cytokines involved in the mediation of resistance to infection was carried out , SAG 1 transgenic mice exhibited low production levels of IL 12 , IFN gamma and TNF alpha in sera during the acute phase of T . gondii infection . ^^^ Moreover , in vitro studies indicated that in SAG 1 transgenic mice IFN gamma and IL 12 production was lower than in their wild type counterparts , although levels of TNF alpha increased in SAG 1 transgenic mice on day 9 after infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Several pro inflammatory cytokines such as Th 1 cytokines ( including IL 2 and IFN gamma ) and TNF alpha have been identified to participate the process of viral clearance and host immune response to HBV . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CB cytokine levels ( IL 4 , IL 5 , IL 6 , IL 10 , IL 12 , IL 13 , TNF alpha and IFN gamma ) and total IgE levels were measured and compared between the two groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The single deletion of IFN gamma or TNF alpha resulted in a severe lesion of multiple necrosis without granuloma , and cytokine mRNA level other than knocked out cytokine was normal , suggesting that IFN gamma and TNF alpha are principally important cytokines . ^^^ In knockout mice for IL 12 or IL 18 , necrotic lesion was not induced after infection and the pathological change was not so significant as in IFN gamma / TNF alpha knockout mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Rotavirus specific subclass antibody responses and cytokines , tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , interleukin 8 ( IL 8 ) , and IL 10 , were measured in children 7 24 months of age with rotavirus diarrhoea ( n = 29 ) ; the responses were compared with children with watery diarrhoea from whom no enteric pathogens were isolated ( controls ; n = 11 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Peanut allergic donors show Th 2 polarization of cytokine production by peanut specific cells ( IFN gamma ( low ) , TNF alpha ( low ) , IL 4 ( high ) , IL 5 ( high ) , IL 13 ( high ) ) . ^^^ Conversely , nonallergic children and children who have outgrown their allergy show Th 1 skewing to peanut antigens ( IFN gamma ( high ) , TNF alpha ( high ) , IL 4 ( low ) , IL 5 ( low ) , IL 13 ( low ) ) , similarly to nonallergenic food antigens ( beta lactoglobulin , OVA ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Similarly , the increase in IFN gamma and TNF alpha production by CD4+ and CD8+ T cells from the spleens of mice undergoing acute GVHD was not observed if the mice were injected with T cells from an MEB treated MLR instead of an untreated MLR . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Fecal IL 1beta and mucosal TNF alpha and IFN gamma concentrations were significantly lower ( P < 0 . 05 ) in verum treated mice than in the placebo group . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma plays important roles in inflammation , usually in synergy with other cytokines , such as IL 1beta and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine mRNA level in the cell cultures was measured by the RT PCR method using 11 primer pairs for the following cytokines : IFN alpha , IFN gamma , IL 1beta , IL 2 , IL 4 , IL 6 , IL 8 , IL 10 , IL 12 , IL 18 and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The levels of TNF alpha and IFN gamma in PHA MNC CM increased in a PHA dose dependent manner and were much higher in the controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These data question the role of systemic TNF alpha and IFN gamma in regulating hepatic dysfunction and suggest a differential functional response of the liver to systemic and gut derived septic events . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Subsequent administration ( therapeutic treatment ) of Histaglobin resulted in a decrease of total and specific serum IgE levels ; total and specific IgG 1 serum levels were reduced by more than 50 % and 45 % , respectively ; the mice displayed a down regulation of IL 4 and TNF alpha serum levels and showed increased levels of IFN gamma and IgG2a . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
ICOSL expression was upregulated by tumour necrosis factor alpha ( TNF alpha ) , whereas interferon gamma ( IFN gamma ) had no such effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We compared the patterns of the pro inflammatory cytokines , interferon gamma ( IFN gamma ) , interleukin 2 ( IL 2 ) and tumor necrosis factor alpha ( TNF alpha ) , and the anti inflammatory cytokines , interleukin 10 ( IL 10 ) and tumor growth factor beta ( TGF beta ) from peripheral blood of male and female patients with relapsing remitting ( RR ) and secondary progressive ( SP ) forms of multiple sclerosis ( MS ) . ^^^ Peripheral blood anti coagulated with heparin was obtained from 47 MS patients ( 30 women and 17 men ) and activated with phorbol 12 myristate 13 acetate ( PMA ) and ionomycin in the presence of brefeldin A and stained for flow cytometry with fluorescently labeled antibodies against intracellular IFN gamma , TNF alpha , IL 2 , IL 4 and IL 10 . ^^^ The secretion of the pro inflammatory cytokines , IFN gamma and TNF alpha , is influenced by gender in MS patients and may contribute to the sexual dimorphism of MS . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neutralizing antibodies against IFN alpha , IFN gamma , and TNF alpha , used separately or together , significantly abrogated the delaying and / or inhibiting action of CM . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have studied the changes in the serum level of the following cytokines during the course of treatment of nosocomial pneumonia ( NP ) : pro inflammatory IL 1alpha and IL 6 , chemoattractive IL 8 , anti inflammatory IL 4 and IL 10 , and differentiating activating IFN gamma and TNF alpha . ^^^ The level of circulating pro and anti inflammatory cytokines , IL 8 , and TNF alpha was decreasing , however , the level of IFN gamma did not change significantly during 15 days . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In order to examine the contribution of the endogenous IFNs and TNF alpha in maintaining innate immunity , specific antibodies against IFN alpha , IFN beta , IFN gamma and TNF alpha were added to VSV infected leukocytes resistant to infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Ascaris antigen specific production of TNF alpha , IL 12 and IFN gamma was low in singly Ascaris as well as in co infected patients , whereas secretion of IL 10 and IL 13 was elevated and similarly high in all patient groups . ^^^ In summary , chronically infected Ascaris and Trichuris patients with a high parasite load presented reduced cellular reactivity and lower type 1 TNF alpha , IFN gamma and IL 12 responses when compared with endemic controls , whereas type 2 IL 10 and IL 13 productions were similar in all groups from the endemic area . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
THP 1 cells were infected using strains of M . tuberculosis and cytokine profiles were determined using immunoassays for cytokines IL 1beta , IL 10 , IL 12 , IFN gamma and TNF alpha . ^^^ Cytokine assays indicate that the Type 1 strain induces comparatively lower titers of IL 1beta , IFN gamma and TNF alpha in infected THP 1 cells as compared to H37Ra and H37Rv strains . ^^^ In vitro secretion of IL 1beta , IFN gamma and TNF alpha is suppressed in Type 1 infected culture samples when compared to H37Ra and H37Rv infected cells . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The mRNA levels of IFN gamma , IL 10 , TNF alpha , TGF beta , and inducible NO synthase in the small intestine and the Peyer ' s patches were determined by semi quantitative RT PCR . ^^^ Mice fed on the cereal based NTP 2000 diet expressed higher levels of the Th 1 type and pro inflammatory markers IFN gamma , TNF alpha , and inducible NO synthase mRNA compared to the Prosobee fed animals . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : MIN 6 and MIN 6 IkappaBalphaM ( Bm ) cells were cultured in mixtures of IL 1beta and TNF alpha or IL 1beta , TNF alpha , and IFN gamma plus / minus the iNOS inhibitor L NMMA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although both strains showed T ( h ) 1 skewed responses on day 4 after infection , significantly higher levels of IFN gamma , tumor necrosis factor ( TNF ) alpha and NO were observed during the course of the infection in BALB / c , suggesting that T ( h ) 1 responses are involved in the resistance . ^^^ Interestingly , in the brain , both strains expressed IFN inducible protein of 10 kDa ( IP 10 ) and monocyte chemotactic protein ( MCP ) 1 genes as early as at 24 h post infection , whereas some differences were observed between both strains thereafter , i . e . enhanced expression of RANTES in C57BL / 6 , and of IFN gamma and TNF alpha in BALB / c respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , synergistic augmentation of ICAM 1 by a combination of TNF alpha and interferon gamma ( IFN gamma ) treatment was completely abrogated on the surface of HEp 2 IkappaB ( ( S32AS36A ) ) cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Because IFN gamma and tumour necrosis factor ( TNF ) alpha trigger a partially overlapping set of antiviral defence mechanisms , it is tempting to speculate that TNF alpha also inhibits HCV replication . ^^^ However , this study shows that TNF alpha does not affect HCV protein and RNA synthesis , nor does it synergistically enhance the inhibitory effect of IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cell death was specific to IFN gamma treatment and did not occur with either IFN alpha or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Colonic mRNA expression for inducible nitric oxide synthase ( iNOS ) , TNF alpha , interferon gamma ( IFN gamma ) and interleukin 4 ( IL 4 ) were measured by reverse transcription polymerase chain reaction ( RT PCR ) , and nuclear factor kappaB ( NF kappaB ) activation was evaluated by electrophoretic mobility shift assay . ^^^ On day seven , mRNA levels of IFN gamma and IL 4 in the colons of TNF alpha / mice were faint or not detected ; in contrast , those of TNF alpha+ / + mice were detected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This reduction in L . major infection is a TNF alpha dependent mechanism as L . major infected Mphi expressed twice as much TNF alpha mRNA as L . amazonensis infected cells , and anti TNF alpha reversed the IFN gamma effect . ^^^ Despite having higher NO production than IFN gamma treated cells , AMG addition to IFN gamma plus TNF alpha treated cells only partially reversed the inhibition in L . major , but not in L . amazonensis infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This effect was due to activation of MHC class 1 restricted CD 8 ( + ) T cells coupled with an increased secretion of proinflammatory cytokines IFN gamma , TNF alpha and IL 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) found at elevated levels in transplant recipients and AIDS patients with retinitis sensitize RPE cells and other retinal cells to FasL mediated apoptosis , thus contributing to retina destruction and necrosis rather than inflammation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lymphocytes from BiAb treated mice showed a significant reduction in their ability to proliferate , and to produce IL 2 , IFN gamma and tumor necrosis factor ( TNF ) alpha , in response to mTg re stimulation compared to lymphocytes from untreated mice . ^^^ Our data suggest that engagement of CTLA 4 expressed on activated autoreactive T cells in close proximity to the thyroid can increase the number of regulatory T cells and their ability to produce TGF beta 1 , with a concomitant reduction in IFN gamma and TNF alpha , resulting in suppression of EAT . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Amelioration of clinical disease is associated with downregulation of both virus and myelin epitope specific Th 1 effector functions ( delayed type hypersensitivity and IFN gamma production ) and the inhibition of CNS mRNA expression coding for the proinflammatory cytokines , TNF alpha , IL 1 beta , and IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The hypothesis could be tested in vitro by comparing the binding of P . falciparum infected HbAS and HbAA erythrocytes to platelet endothelial cell adhesion molecule 1 ( CD 31 ) and by comparing the levels of tumor necrosis factor ( TNF ) and interferon gamma ( IFN gamma ) following in vitro stimulation of PBMCs by HbAS and HbAA infected erythrocytes . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Other aspects of immune function , ie DTH responses , NK cell activity , lymphocyte proliferation and production of TNF alpha , IL 1 beta , IL 6 , IFN gamma , IL 2 , IL 4 , and PGE ( 2 ) , were not affected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we investigated whether CD 69 expression on human neutrophils could be modulated by inflammatory or anti inflammatory cytokines ( IL 1beta , IL 2 , IL 4 , IL 6 , IL 8 , IL 10 , IL 12 , IL 18 , G CSF , GM CSF , TNF alpha , TGF beta , IFN alpha , IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine genotypes and mRNA transcript levels of IL 2 , TNF alpha , TGF beta 1 , IL 10 , IL 6 and IFN gamma were determined using peripheral blood cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In smooth muscle cells ( SMCs ) , fractalkine is induced by proinflammatory cytokines [ tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) ] , which may mediate monocyte adhesion to SMCs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , it increases the IFN gamma or LPS induced expression of inducible NO synthase , TNF alpha , IL 1beta , and IL 6 genes and the secretion of these cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Coculture of T cells with IL 12 enhanced the secretion of IFN gamma , but not TNF alpha , whereas coculture with IL 18 enhanced the secretion of TNF alpha , but not INF gamma . ^^^ However , coculture with both IL 18 and IL 12 induced high levels of both TNF alpha and IFN gamma . ^^^ Finally , estrogen treatment , previously found to inhibit both TNF alpha and IFN gamma production , completely abrogated all signs of passive EAE . ^^^ These data demonstrate that optimal potentiation of encephalitogenic activity can be achieved by conditioning MOG specific T cells with the combination of IL 12 and IL 18 , which , respectively , induce the secretion of IFN gamma / CCR5 and TNF alpha / CCR4 / CCR7 , and that estrogen treatment , which is known to inhibit both proinflammatory cytokines , can completely ablate this aggressive form of passive EAE . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 10 , IL 12 , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) were measured . ^^^ RESULTS : Significantly higher levels of IL 2 , IL 6 , IL 8 , IL 10 , IFN gamma , and TNF alpha were detected in the eighth instillation as compared to the first instillation ( p < 0 . 001 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We studied the role of NF kappaB activation in pancreatic islet cell death by using a gamma interferon ( IFN gamma ) / TNF alpha synergism model we had previously reported . ^^^ IFN gamma pretreatment did not affect TNF alpha induced NF kappaB activation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma and TNF alpha expression were also higher in the rejection group . ^^^ CONCLUSIONS : Early transplant rejection is associated with increased MUC 2 , MUC 4 , IFN gamma , and TNF alpha expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This protection was associated with suppression of systemic levels of IFN gamma and TNF alpha , inhibition of macrophage infiltration in islets , restoration of islet insulin secretion , and reduction of beta cell apoptosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this report , we demonstrate that NADPH oxidase is activated by tumor necrosis factor alpha ( TNF alpha ) plus interferon gamma ( IFN gamma ) in human monocytic cells ( THP 1 cells ) differentiated with phorbol ester ( PMA ) and that physiological concentration of 17beta estradiol inhibits NADPH oxidase activity in THP 1 cells stimulated with TNF alpha plus IFN gamma . ^^^ Activation of NADPH oxidase induced by TNF alpha plus IFN gamma is caused by up regulation of p 47 ( phox ) ( cytosolic component of NADPH oxidase ) expression . 17beta Estradiol prevents the up regulation of p 47 ( phox ) mRNA and protein expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
T cells were stained by monoclonal antibodies directed against the surface markers CD 4 , CD 69 and against the intracellular cytokines interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) , interleukin 4 ( IL 4 ) and IL 10 . ^^^ RESULTS : After antigen specific stimulation with the G 1 protein , the CD4+ T cells of 30 AS patients ( 61 . 7 % ) and of 12 RA patients ( 54 . 5 % ) secreted significant amounts of IFN gamma and TNF alpha , while , in contrast , only 10 % of the normal controls showed a response ( P < 0 . 05 ) . ^^^ In contrast , stimulation with HC gp 39 and collagen 2 showed no significant IFN gamma and TNF alpha secretion of MNC in all groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
During the chronic phase of infection , parasites persisted at high levels in gp91phox ( / ) mice , and cutaneous lesions re emerged in approximately 60 % of these mice . gp91phox deficiency did not impair the expression of iNOS or the production of TNF and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We report here the standardized conditions for stimulation of macaque whole blood samples with various protein or peptide antigens , and production of significant intracellular levels of interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) in CD4+ as well as CD8+ T lymphocytes . ^^^ We observed significantly higher levels of TNF alpha compared with IFN gamma in both CD4+ and CD8+ T lymphocytes from all the macaque whole blood samples stimulated with staphylococcal enterotoxin B ( SEB ) as an antigen . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Biopsies from 19 patients were stained with HE and Giemsa , and serial sections were immunohistochemically stained using CD45RO , CD 20 , CD 4 , CD 8 , CD 68 , CD1a , CD 34 , IL 4 , IL 10 , TNF alpha and IFN gamma antibodies . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF enhances CD4+ T cell alloproliferation , IFN gamma responses , and intestinal graft versus host disease by IL 12 independent mechanisms . ^^^ TNF inhibition reduced T cell proliferation and IFN gamma production in supernatants of MLC using either B6 . ^^^ Furthermore , TNF inhibition was associated with a reduction in colonic GVHD scores ( p = 0 . 039 ) and in the percentage of the splenic CD 4 ( + ) T cells that expressed IFN gamma ( 16 vs 6 % ) . ^^^ These findings indicate that TNF promotes CD 4 ( + ) T cell alloproliferation , IFN gamma responses , and intestinal GVHD by IL 12 independent mechanisms . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Con A induced hepatitis is a liver specific inflammation mediated by activated T cells and is driven by an up regulation of the hepatic expression of TNF alpha , IFN gamma , and IL 4 . ^^^ Furthermore , CCL2 / MCP 1 neutralization was associated with a significant reduction in the hepatic levels of TNF alpha and IFN gamma , but with a significant increase in hepatic IL 4 levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We used quantitative , competitive RT PCR to analyze the number of TNF alpha , IFN gamma , MMP , and TIMP mRNA transcripts and western blotting to analyze MMP and TIMP protein production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The time profiles of mRNA expression for IL 1beta , IL 2 , IL 4 , IL 10 , IFN gamma , TNFalpha were similar to that of iNOS , while TGFbeta 2 , IL 12p35 , and IL 12p40 demonstrated a reverse pattern . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast with SP A ( + / + ) mice , rHuKGF failed to suppress the high levels of TNF alpha , IFN gamma , and nitric oxide contained in bronchoalveolar lavage fluids collected on day 7 after BMT from SP A ( / ) mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum was harvested and stored at 80 degrees C for analysis of cortisol ( CS ) , corticosteroid binding globulin ( CBG ) , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) . ^^^ Time 10 Treatment 10 Diet interactions were also observed for serum concentrations of TNF alpha ( P=0 . 084 ) and IFN gamma ( P=0 . 022 ) such that both the TNF alpha and IFN gamma response to the LPS challenge was lower in those pigs receiving the MFO diet as compared to the LPS treated pigs on the Cont diet . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Whole blood samples of 3 of 11 healthy individuals demonstrated a marked suppression of IL 2 mRNA expression ( > 50 % ) and a partial inhibition of IL 4 , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) mRNA expression on addition of CsA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha and IFN gamma gamma were more potent at inducing FKN secretion than was IL 1beta . ^^^ Finally , incubation of fibroblasts with the invasive strain of E . coli inhibited TNF alpha and IFN gamma induced production of FKN . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Containment of intracellularly viable microorganisms requires an intricate cooperation between macrophages and T cells , the most potent mediators known to date being IFN gamma and TNF . ^^^ Compared with wild type mice , LT beta R KO mice had similar transcript levels of TNF and IFN gamma and recruited similar numbers of CD 3 ( + ) T cells inside granulomatous lesions in M . tuberculosis infected lungs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Bacterial plate counts in B 6 mice treated with clodronate liposomes were unchanged at 3 days and were higher in control treated mice at 5 days postinfection ( p . i . ) , whereas in BALB / c mice , bacterial load was significantly elevated in the cornea of mice treated with clodronate liposomes at both 3 and 5 days p . i . mRNA expression levels for pro ( IFN gamma and TNF alpha ) and anti ( IL 4 and IL 10 ) inflammatory cytokines also were determined in BALB / c mice treated with clodronate liposomes vs control treated mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Combining large and small scale ELISPOT assays for expression cloning has the following advantages compared to conventional cDNA expression cloning : 1 ) the number of recombinant plasmids which can be screened is greater than 10 , 000 per well in a 24 well plate in a large scale ELISPOT assay compared to fewer than 100 per well in a 96 well plate in an IFN gamma ELISA or a TNF alpha bioassay ; 2 ) the total number of recombinant plasmids which can be screened in a routine assay is 2 10 10 ( 5 ) in only one 24 well plate in a large scale ELISPOT assay compared to 1 10 10 ( 5 ) in ten 96 well plates in an IFN gamma ELISA or a TNF alpha bioassay . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Accordingly , CD8+ T cells were tested for cytotoxic activity and secretion of TNF alpha , while secretion of IFN gamma , IL 5 and IL 4 was determined for CD4+ T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Among immune donors , the percent of both CD 3 lymphocytes and NK cells expressing IFN gamma after 24h of T27K was increased above control ( p < 0 . 05 ) , while the percent of NK cells producing TNF alpha in response to T27K was not greater than control . ^^^ Depletion of NK cells from peripheral blood mononuclear cells resulted in significant increases in TNF alpha and IL 10 ( for both , p < 0 . 05 ) but resulted in no significant decrease in IFN gamma or IL 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha and IFN gamma are overexpressed in the bone marrow of Fanconi anemia patients and TNF alpha suppresses erythropoiesis in vitro . ^^^ In Fanconi anemia ( FA ) C mice tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) have key roles in the pathogenesis of bone marrow failure . ^^^ In acquired aplastic anemia , a disease in which , similarly to FA , marrow failure occurs , TNF alpha and IFN gamma act as late mediators of the stem cell damage and are overexpressed in patient marrow lymphocytes . ^^^ This study evaluated in marrow mononuclear cells ( MNCs ) of patients with FA , the expression of negative modulators of the hematopoiesis , such as TNF alpha , IFN gamma , macrophage inflammatory protein 1alpha ( MIP 1alpha ) , and surface Fas ligand , and the role of TNF alpha on FA erythropoiesis in vitro . ^^^ TNF alpha and IFN gamma were significantly overexpressed in stimulated marrow MNCs of FA patients as compared to healthy controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A principal component analysis of IFN gamma , TNF alpha , and TGF beta reflected the immunologic model of TB . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
But IFN gamma and TNF alpha producing cells were observed sporadically in chronic HBV patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Patients who developed moderate to severe acute GVHD had higher levels of TNF alpha , IFN gamma , IL 10 and sFas at 2 weeks post SCT than in those with less GVHD . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
LPS induced plasma levels of IL 6 , IL 1beta , and TNF alpha were also significantly higher in iron preloaded rats as shown by ELISA , but IFN gamma levels were unchanged . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both in IFN gamma ( + / ) and IFN gamma ( / ) NOD mice , IL 12 administration generates a massive and destructive insulitis characterized by T cells , macrophages , and CD11c ( + ) dendritic cells , and increases the number of pancreatic CD 4 ( + ) cells secreting IL 2 and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although hepatic TNF alpha mRNA and circulating TNF alpha were significantly elevated in LPS challenged IRF 2 ( / ) mice , levels of IL 1 , IL 12 , and IFN gamma mRNA and protein , as well as IL 6 protein , were significantly lower than levels seen in LPS challenged IRF 2 ( + / + ) mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Compared with peripheral blood mononuclear cells ( PBMC ) of the patients , TIL produced higher levels of IL 1beta , IL 6 , IL 10 , TNF alpha and TGF beta , whereas their production of IL 12 and interferon gamma ( IFN gamma ) was only slightly higher than that in PBMC . ^^^ After 1 week of therapy , the cytokine production by OSC cells had largely decreased , while the production of TNF alpha , IFN gamma , TGF beta and IL 12 by TIL had increased greatly , although other cytokine levels were almost constant during the investigations . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
From a wealth of rodent studies using live infection models , the following conclusions can be drawn : ( 1 ) neutralization or gene deletion for TNF alpha is frequently associated with reduction of host defense in models of live Gram positive or Gram negative infections as well as infection by intracellular microbes such as Salmonella and Listeria ; ( 2 ) absence of the IL 1 receptor can also result in decreased resistance to Listeria or Gram positive bacteria and ( 3 ) TNF alpha and IFN gamma are required for defense against infection caused by Mycobacterium tuberculosis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHOD OF STUDY : Serum Th 1 ( IFN gamma , TNF alpha ) and Th 2 cytokine ( IL 4 , IL 10 ) levels were assessed by ELISA methods ( n = 9 ) and peripheral blood Th1 / Th2 lymphocyte ratios ( n = 4 ) by flow cytometry before and after MIVIg treatments in women with four or more consecutive RSA . ^^^ RESULTS : Pre treatment serum IFN gamma ( 0 . 06 + / 0 . 09 pg / mL , mean + / SD ) , TNF alpha ( 0 . 21 + / 0 . 45 pg / mL ) , IL 4 ( 0 . 70 + / 1 . 16 pg / mL ) , and IL 10 ( 1 . 12 + / 1 . 67 pg / mL ) increased to 0 . 17 + / 0 . 16 pg / mL , 0 . 77 + / 0 . 28 pg / mL , 1 . 82 + / 0 . 89 pg / mL , and 3 . 44 + / 0 . 48 pg / mL , respectively , after MIVIg treatments ( P < 0 . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunostimulatory effects of isolates were tested on induction of cytokine ( tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) ) synthesis in primary cultures of human peripheral blood mononuclear cells ( PBMC ) isolated from a buffy coat . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Monocytes produced NE constitutively , with little regulation by cytokines IL 1beta , TNF alpha , or IFN gamma but released it when stimulated by CD 40 ligand , a cytokine found in atheroma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , the cumulative nociceptive response time for intrathecal ( i . t . ) injection of TNF alpha ( 100 pg ) , IL 1 beta ( 100 pg ) , IFN gamma ( 100 pg ) , substance P ( 0 . 7 microg ) or glutamate ( 20 microg ) was dose dependently diminished by decursinol . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHOD : The amounts of IFN alpha , IFN gamma , TNF alpha , IL 8 in the peripheral blood mononuclear cells ( PBMCs ) culture supernatants deal with by different concentrations of LianBiZhi ( LBZ ) injection made of andrographolide were detected by biological activity test or ELISA in vitro . ^^^ RESULT : The LBZ injection could promote IFN alpha , IFN gamma , TNF alpha inductions of PBMCs , but had no effect on IL 8 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Some correlative immunological indexes were measured , including delayed type hypersensitivity ( DTH ) reaction , anti collagen and anti Mycobacterium tuberculosis ( MT ) antibody in serum , and levels of IFN gamma and TNF alpha in articular steep in rats . ^^^ In addition , the expression of IFN gamma and TNF alpha in joints were locally downregulated . ^^^ Its remedial mechanisms are likely related to the downregulation of both IFN gamma and TNF alpha , and the suppression of cell immunity . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We looked for the presence of activated DR+ inflammatory cells and the expression patterns , localization , and immunostaining identification of genes for cytokines ( IL 1beta , TNF alpha , IL 6 , and IFN gamma ) , MCP 1 , and iNOS in the left ventricle biopsies from 2 vessel disease anginal patients , 24 with UA and 12 with stable angina ( SA ) , who underwent coronary bypass surgery . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Among the cytokines inhibited were interleukin 4 ( IL 4 ) , IL 13 , IFN gamma , tumour necrosis factor alpha ( TNF alpha ) and granulocyte macrophage colony stimulating factor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
On day 7 , neutropenic mice had significantly higher lung concentrations of IL 10 , TNF alpha , IL 4 , and IL 12 than control mice , but there was no difference in IFN gamma and MCP 1 levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
BACKGROUND : To find out whether preterm labor is associated with raised maternal serum concentrations of interleukin ( IL ) 6 , IL 8 , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) and whether the measurement of these cytokines can be used to detect early intrauterine infection in preterm labor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MEASUREMENTS : Concentrations of interleukin 6 ( IL 6 ) , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) , and leukocytes , platelets and organ pathology were evaluated . ^^^ RESULTS : Burn and LPS showed an additive effect on the release of IL 6 but not of TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Standard blocking assays were performed in the presence of anti interferon gamma ( IFN gamma ) , anti tumor necrosis factor alpha ( TNF alpha ) , and anti FasL . ^^^ The CD 8 ( + ) antiviral effect was markedly reduced by blocking either IFN gamma or FasL but was unaffected by blocking TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Active immunization of C57BL / 6 mice with Peptide 25 can induce the development of CDT 4 ( + ) TCRVbeta 11 ( + ) and CDT 4 ( + ) TCRVbeta 11 ( ) Th 1 cells that produce IFN gamma and TNF alpha , and protects against subsequent infection with live Mycobacterium tuberculosis H37Rv IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Early changes in glucose and phospholipid metabolism following apoptosis induction by IFN gamma / TNF alpha in HT 29 cells . ^^^ Significantly increased lactate and NTP ( all nucleoside 5 ' triphosphates ) signals were detected 4 h after apoptosis inducing IFN gamma / TNF alpha treatment , but not in cells which were TNF alpha treated without IFN gamma preincubation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Previously , the production of the cytokines TNF alpha and IFN gamma in T cells from children with CMA has been shown to be decreased , and the production of IL 4 has been shown to be increased when compared to healthy children . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present work , at physiological ( 15ng / ml ) and stress induced levels ( 30ng / ml ) of prolactin , interferon gamma ( IFN gamma ) and interleukin ( IL ) 12 p 70 levels , but not of IL 10 and tumor necrosis factor alpha ( TNF alpha ) , increased significantly ( p < 0 . 05 0 . 006 ) in phytohemeagglutinin ( PHA ) +lipopolysaccharide ( LPS ) stimulated whole blood . ^^^ On the other hand , in LPS stimulated whole blood , prolactin enhanced significantly ( p=0 . 027 ) the production levels of IL 10 , but not of IFN gamma , IL 12 p 70 , and TNF alpha , in non concentration dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
ASMC were incubated for 24 hours with various concentrations of T ( H ) 2 cytokines ( IL 4 , IL 5 , IL 10 , and IL 13 ) ; transforming growth factor ( TGF ) beta 1 , TGF beta 2 , or TGF beta 3 ; and IL 1beta or TNF alpha with or without IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : ATG treated patients showed decreases in the expression of cytotoxic T cell genes perforin ( 56 % , p = 0 . 03 ) and granzyme B ( 45 % , p = 0 . 01 ) and cytokine gene IFN gamma ( 75 % , p = 0 . 005 ) , and significant increases in the expression of cytokine genes IL 7 ( 550 % , p = 0 . 04 ) , IL 10 ( 275 % , p = 0 . 01 ) , IL 15 ( 417 % , p = 0 . 03 ) , TNF alpha ( 615 % , p = 0 . 01 ) , and TGF beta ( 235 % , p = 0 . 02 ) . ^^^ CONCLUSION : ATG induced decreases in the expression of IFN gamma , perforin , and granzyme B and increases in IL 10 and TGF beta might be considered beneficial to the recipient , whereas increases in the expression of IL 7 , IL 15 , and TNF alpha genes might be involved in immunological processes not effected by ATG that may harm the transplant in the long term . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The anti inflammatory role of these regulators in rheumatoid arthritis is highlighted by their induction with IFN gamma and dexamethasone , whilst the pro inflammatory cytokine TNF alpha had no effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , in untreated allografts , IL 2 , TNFalpha and IFN gamma were strongly expressed , an effect that markedly decreased after simulect treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : RDP 58 led to decreased TNF and IFN gamma ( but not IL 1beta ) production by biopsies and lamina propria mononuclear cells from CD patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Although IFN gamma did not directly induce the expression of eotaxin protein , it increased the induction by TNF alpha when these cytokines were added simultaneously . ^^^ In contrast , preincubation of cells with IFN gamma for 24 hours profoundly inhibited the production induced by TNF alpha . ^^^ IFN gamma did not influence the TNF alpha induced binding of nuclear factor kappaB to a DNA probe derived from the eotaxin promoter . ^^^ IFN gamma did not increase the ability of TNF alpha to activate the eotaxin promoter . ^^^ Studies of eotaxin mRNA levels indicate that IFN gamma combined with TNF alpha increased the expression of eotaxin mRNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1beta , IFN gamma and TNF alpha increase vulnerability of pancreatic beta cells to autoimmune destruction . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , the role of two inflammatory mediators , previously identified in the tear film of ocular allergy subjects , TNF alpha and IFN gamma , were evaluated for their effect on the release of two chemotactic mediators , IL 8 and RANTES , from cultured human conjunctival epithelial cells . ^^^ METHODS : Human conjunctival epithelial cells ( primary cells or HC 0597 cell line ) were grown to confluence and stimulated with various concentrations of TNF alpha , IFN gamma , or a combination of both . ^^^ Treatment of HC 0597 cells with both TNF alpha and IFN gamma resulted in a synergistic increase in the release of RANTES . ^^^ CONCLUSIONS : Stimulation of conjunctival epithelium with proinflammatory mediators , TNF alpha and / or IFN gamma , generated the release of the chemotactic factors IL 8 and RANTES , which could act to prolong inflammation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Besides its well known hemorheological properties PTX has been found to decrease the secretion of some inflammatory cytokines such as TNF , IL 12 and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We determined the role of TNF alpha and IFN gamma as well as different T cell populations in infection with these Salmonella strains . ^^^ After oral inoculation , control of plasmid cured bacteria substantially relied on both IFN gamma and TNF alpha signaling while control of SPI 2 mutants did not . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Frequencies of parasite specific IFN gamma producing T cells , intracerebral and splenic IFN gamma production , and T . gondii specific IgM and IgG titers in LTalpha ( / ) and TNF / LTalpha ( / ) mice were reduced only early after infection . ^^^ In addition , TNF ( / ) , LTalpha ( / ) , and TNF / LTalpha ( / ) , but not WT , mice succumbed to infection with the highly attenuated ts 4 strain of T . gondii or to a subsequent challenge infection with virulent RH toxoplasms , although they had identical frequencies of IFN gamma producing T cells as compared with WT mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Changes in the local expression of IL 1beta , IL 2 , IL 4 , IL 6 , IL 8 , IL 10 , IL 12 , IL 15 , IL 18 , TNF alpha , IFN gamma and TGF beta ( 2 ) genes in the uterine adnexa and endometrium were studied in women with pyoinflammatory adnexal diseases . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
BACKGROUND : The aim of the present study was to further investigate the characteristics of both graft infiltrating cells and splenocytes during acute vascular rejection ( AVR ) , cell mediated rejection and non rejection of vascularized concordant xenografts , by analysing both proinflammatory [ interleukin 1beta ( IL 1beta ) and tumour necrosis factor ( TNF alpha ) ] and more specific [ ( IL 2 , IL 4 , IL 10 , IL 12p40 and interferon gamma ( IFN gamma ) ] cytokines . ^^^ RESULTS : All the studied cytokines ( IL 1beta , IL 2 , IL 4 , IL 10 , IL 12p40 , IFN gamma and TNF alpha ) were up regulated in the grafts from rejecting untreated ( day 2 ) and DSG treated animals ( day 8 ) in comparison with grafts from CyA + DSG treated animals ( day 8 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To elucidate the mechanism responsible for the adoptive immunogene therapy , serum IL 4 , IL 6 , IFN gamma , and tumor necrosis factor alpha production in Th1 / Th2 cytokine balance and quantification of tumor vascularity were investigated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These studies demonstrate that ( a ) OX40L plays a role in sustaining the long term proliferation of CD 8 ( + ) T cells in addition to the known effect on CD 4 ( + ) T cells following activation , ( b ) OX40L enhances the production of Th 1 cytokines ( IL 2 , IFN gamma , and TNF alpha ) from both CD 4 ( + ) and CD 8 ( + ) while no change in IL 4 expression was observed , and ( c ) the anti apoptotic effect of OX40L on T cells is likely the result of sustained expression of anti apoptotic genes while genes involved in apoptosis are inhibited . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
All transformed gammadelta T cells analyzed , regardless of their compartment of origin , strongly expressed intracytoplasmic IFN gamma and TNF alpha , but little IL 2 or anti inflammatory IL 4 or IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Single TNF alpha expressing CD4+ and CD8+ T cells were likewise reduced and shifted towards IFN gamma / TNF alpha co production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Peripheral blood cells ( PB ) were stimulated with either ' self ' or ' non self ' preparations of faecal samples and isolated Bacteroides fragilis group organisms ( Bfg ) or Escherichia coli ( Esch . coli ) , and pro and anti inflammatory cytokines ( IL 10 , IL 4 , IL 6 , IFN gamma , TNF alpha ) were measured in the culture supernatant . ^^^ Additionally , TNF alpha , IL 6 and in part IFN gamma cytokine secretion by PB cells following stimulation with whole stool preparations and single members of the flora was significantly decreased , whereas the IL 10 and in part IL 4 cytokine secretion was increased at the end of the study . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
One of the two CTL resistant cell lines became susceptible after treatment with IFN gamma and TNF alpha , while the other was lysed only after pulsing with HA 1 ( H ) peptide . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Concentrations of neopterin , nitrate , tryptophan , IFN gamma , and tumor necrosis factor alpha ( TNF alpha ) were determined at various times during the first 48 hours in 11 patients who received intraperitoneal rhIL 12 in doses ranging from 100 to 1 , 500 ng / kg . ^^^ Increased concentrations of TNF alpha and IFN gamma were detected in 3 of 9 and 8 of 11 patients , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In a previous study we showed that recipients of IFN gamma gko grafts develop high levels of LPS induced TNF alpha release . ^^^ When our current data are viewed in the context of this observation , they suggest that intestinal epithelial cell apoptosis in the parent > F 1 hybrid model of acute GVHD is mediated primarily by nitric oxide rather than TNF alpha , and that this depends on donor derived IFN gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The inhibition of CIA by oral administration of proteoglycan was associated with decrease in anti CII IgG and IgG2a antibodies ( Abs ) as well as varying kinds of cytokines including IL 12 , TNF alpha , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PPVO induces IL 12 , TNF alpha and , together with a suboptimal concentration of Concanavalin A , IFN gamma in human peripheral blood leukocytes as well . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Experimental data have shown the critical role of pro inflammatory cytokines like tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) in the development of liver injury . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we examined the effect of zinc deficiency on IL 2 and IFN gamma in HUT 78 ( Th 0 ) and D1 . 1 ( Th 1 ) cell lines and TNF alpha , IL 1 beta , and IL 8 in the HL 60 ( monocyte macrophage ) cell line . ^^^ These data demonstrate that zinc mediates positively the gene expression of IL 2 and IFN gamma in the Th 1 cell line and negatively TNF alpha , IL 1 beta , and IL 8 in the monocyte macrophage cell line . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : The percentage of T lymphocytes primed for TNF alpha , IFN gamma and IL 4 was higher in blood samples from patients than from healthy subjects ; the difference was statistically significant for TNF alpha producing cells ( p=0 . 01 ) . ^^^ The percentage of TNF alpha , IFN gamma and IL 4 primed cells was higher in patients with type 6 plaques ( complicated lesions ) than in patients with type 5 plaques ( less complicated lesions ) . ^^^ CONCLUSION : Our results suggest a relationship between the percentage of circulating T lymphocytes expressing TNF alpha and possibly IFN gamma and IL 4 and the histological type of atherosclerotic plaque in patients with carotid artery disease . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immediately after the formaldehyde exposure , murine alveolar macrophages were harvested from the mice . 18 hrs after LPS stimulation , the cytokines ( IL 4 , IL 10 , IFN gamma , TNF alpha ) were measured in the supernatant of cultured murine alveolar macrophages using the ELISA method . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Concomitant with these phenotypic changes , naive cells acquired the ability to produce interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) , expressed perforin and granzyme B , and acquired cytotoxic properties . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment with VG 312 strongly reduced secretion of Th 1 cytokines ( TNF alpha and IFN gamma ) produced in response to MOG 35 55 peptide , and to a lesser degree purified protein derivative and Con A , but had no inhibitory effect on serum Ab levels to MOG 35 55 peptide . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
At 10 ( 4 ) M , resveratrol strongly inhibited PHA stimulated IFN gamma and TNF alpha release from PBMC , but it did not cause inhibition at 10 ( 5 ) or 10 ( 7 ) M . ^^^ The concomitant immune effects of resveratrol on PBMC proliferation and release of IFN gamma and TNF alpha may be explained by an inhibitory effect on transcription factor NF kappaB . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Polymorphism of TNF alpha ( 308 A / G ) , IL 10 ( 1082 A / G , 819 C / T 592 A / C ) , IL 6 ( 174 G / C ) , and IFN gamma ( 874 A / T ) ; genetically conditioned cytokine synthesis level in children with diabetes type 1 ] . ^^^ AIM : The aim of our research was identification of the genetically controlled TNF alpha , IL 10 , IL 6 , IFN gamma secretion profile in children with diabetes type 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha and IFN gamma showed lesser stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To investigate the role that cytokines may have in the development of toxoplasmic encephalitis ( TE ) , the levels of gamma interferon ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 12 ( IL 12 ] p 40 [ ) , IL 10 , IL 6 , IL 4 , and IL 2 in serum were examined in CBA / Ca mice infected with a type 2 strain ( ME 49 or FORT ) of Toxoplasma gondii . ^^^ From weeks 1 to 8 of infection , the levels of IL 6 , IL 10 , IL 12 , IFN gamma , and TNF alpha in serum were significantly higher in the ME 49 infected mice than in the FORT infected mice , suggesting a role for these cytokines in the severity of TE in CBA / Ca mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increased expression of erythropoiesis inhibiting cytokines ( IFN gamma , TNF alpha , IL 10 , and IL 13 ) by T cells in patients exhibiting a poor response to erythropoietin therapy . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When used to prime the human monocytic cell line MonoMac 6 , the production of TNF alpha and interleukin 6 ( IL 6 ) was approximately five times greater in cells primed with IFN gamma than unprimed cells . ^^^ Two LPCAT inhibitors SK & F 98625 ( diethyl 7 ( 3 , 4 , 5 triphenyl 2 oxo 2 , 3 dihydro imidazole 1 yl ) heptane phosphonate ) and YM 50201 ( 3 hydroxyethyl 5 , 3 ' thiophenyl pyridine ) strongly inhibited ( up to 90 % ) TNF alpha and IL 6 production in response to LPS in both unprimed MonoMac 6 cells and in cells primed with IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PURPOSE : To examine the regulatory effects of interferon ( IFN ) alpha , IFN gamma , transforming growth factor ( TGF ) beta , and tumor necrosis factor ( TNF ) alpha on human fetal retinal pigment epithelial ( HFRPE ) cell induced apoptosis of human Jurkat T ( Jkt ) cells . ^^^ The cells were precultured with medium alone or with addition of IFN alpha , IFN gamma , TNF alpha , or TGF beta for 72 hours . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
GXM enhanced the secretion of IL 10 and IL 4 , while it reduced the production of pro inflammatory cytokines TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Phasic upregulation of gene expression for chemokines T cell activation gene 3 ( TCA 3 ) / CCL1 , monocyte chemoattractant protein 1 ( MCP 1 ) / CCL2 , macrophage inflammatory protein 1 alpha ( MIP 1alpha ) / CCL3 , MIP 1beta / CCL4 , regulated on activation normal T cell expressed and secreted ( RANTES ) / CCL5 and MIP 2 / CXCL2 3 as well as cytokines tumour necrosis factor alpha ( TNF alpha ) , beta , LT beta , interferon gamma ( IFN gamma ) and transforming growth factor beta 1 ( TGF beta 1 ) in the CNS was observed during attacks of ChREAE . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Vdelta 1 Calpha+ T cell clones spontaneously secrete interferon gamma ( IFN gamma ) and were further induced to release tumour necrosis factor ( TNF alpha ) when triggered by anti CD 3 plus phorbol ester . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine profiles patterns in the expression of interleukin 4 ( IL 4 ) , interleukin 10 , interleukin 12 , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) were therefore studied during the natural acquisition of immunity to Plasmodium falciparum and P . vivax among individuals from Buenaventura , a meso endemic region on the Pacific Coast of Colombia . ^^^ In general , specific type 1 immune responses , characterized by IFN gamma expression , were more likely to develop during P . falciparum infection , whereas pro inflammatory cytokine profiles ( with TNF alpha expression ) were observed more frequently among the P . vivax infections . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of primary human and murine T cells with TIP in vitro resulted in the secretion of IFN gamma , TNF alpha , and IL 10 , whereas in vivo TIP had a protective effect in a mouse acute graft versus host disease ( GVHD ) model . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 2 boluses transiently increased the levels of IL 6 , IFN gamma , TNF alpha and IL 1 beta , with increases in IL 6 and IFN gamma being dose dependent . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the lung , protein secretion is regulated by glucocorticoids , but also proinflammatory cytokines , such as interferon gamma ( IFN gamma ) and TNF alpha , have been found to modulate the expression of this peptide . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
T cell cytotoxicity of human Schwann cells : TNFalpha promotes fasL mediated apoptosis and IFN gamma perforin mediated lysis . ^^^ In a coculture model , Sc exposed to TNFalpha or IFN gamma were able to induce both apoptotic and lytic injury of T lymphocytes . ^^^ Inactivation of fasL with the neutralizing antibody NOK 2 abolished T cell apoptosis induced by Sc treated with TNFalpha , but not by Sc treated with IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunohistochemical staining revealed that Fas and TNF alpha were localized in both normal and regressing luteal bodies , but IFN gamma was localized only in regressing luteal bodies . ^^^ Apoptosis was induced in primary cultured luteal cells , when they were pretreated with TNF alpha and IFN gamma and then incubated with TNF alpha , IFN gamma , and mouse recombinant FasL ( rFasL ) . ^^^ However , no apoptosis was detected in the cells , when they were treated with rFasL alone , TNF alpha alone , IFN gamma alone , TNF alpha and rFasL , IFN gamma and rFasL , or TNF alpha and IFN gamma . ^^^ Fas mRNA expression in cultured luteal cells was up regulated by the treatment of TNF alpha , IFN gamma , or TNF alpha and IFN gamma . ^^^ The expression of FasB mRNA was down regulated , when the cells were treated with TNF alpha and IFN gamma , but its expression was not changed by the treatment of TNF alpha alone or IFN gamma alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Glial cells can release deleterious compounds such as proinflammatory cytokines ( TNF alpha , Il 1beta , IFN gamma ) , which may act by stimulating nitric oxide production in glial cells , or which may exert a more direct deleterious effect on dopaminergic neurons by activating receptors that contain intracytoplasmic death domains involved in apoptosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We examined the temporal expression of IL 1beta , IL 4 , IL 6 , IL 10 , IL 12p70 , GM CSF , IFN gamma , TNF alpha , macrophage inflammatory protein 2 , KC , macrophage inflammatory protein 1alpha , and monocyte chemoattractant protein 1 directly from the tibiotarsal joint in arthritis resistant C57BL / 6 ( B 6 ) and susceptible C3H / He ( C3H ) mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here we use immunocytochemistry , RT PCR , and ELISA to show that under standard growth conditions multipotent neural progenitor cells from humans express multiple cytokines including IL 1alpha , IL 1beta , IL 6 , TGF beta 1 , TGF beta 2 , TNF alpha , but not IL 2 , IL 4 , or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When wild type and PAFR ( / ) mice were compared , there were no marked changes in the kinetics of the expression of MCP 1 , RANTES , IFN gamma and TNF alpha in heart tissue of infected animals . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CONCLUSION : SP might aggravate AD by increasing the production of TNF alpha and IL 10 rather than by affecting IL 4 and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In Crohn ' s disease ( CD ) CD 4 ( + ) T cells producing tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) are important for disease progression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The immunomodulating capacity of alpha MSH is primarily because of its effects on melanocortin receptor ( MC 1R ) expressing monocytes , macrophages , and dendritic cells ( DCs ) . alpha MSH down regulates the production of proinflammatory and immunomodulating cytokines ( IL 1 , IL 6 , TNF alpha , IL 2 , IFN gamma , IL 4 , IL 13 ) as well as the expression of costimulatory molecules ( CD 86 , CD 40 , ICAM 1 ) on antigen presenting DCs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
E . coli mpk induced colitis was associated with increased IFN gamma , TNF alpha , CD 14 , and IL 10 mRNA expression in the colon . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
T helper 1 ( Th 1 ) cytokines [ tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) ] cause inflammation and together are thought to threaten the maintenance of pregnancy . ^^^ We evaluated TNF alpha , IFN gamma , fgl 2 as well as IL 12 messenger RNA ( mRNA ) expression in decidual samples of all mice by quantitative , real time polymerase chain reaction ( PCR ) . ^^^ In stressed mice compared with controls , we observed on day 13 . 5 up regulated TNF alpha , unchanged IFN gamma down regulated fgl 2 , and a slightly increased levels of IL 12 . ^^^ In the IL 12 triggered abortion model , we observed up regulated levels of TNF alpha , IFN gamma and fgl 2 . ^^^ A physiological cascade associated with up regulation of TNF alpha , and an IL 12 triggered cascade characterized by persistent up regulation of TNF alpha and IFN gamma as well as a persistent increase in fgl2 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It is possible that increased IFN gamma production induced by TNF alpha when 179T is present causes CD 4 cell depletion by apoptosis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PROBLEM : Fetal loss syndrome ( abortion / resorption ) occurring on or after gestation day ( gd ) 9 . 5 in CBA / JxDBA / 2 matings is dependent upon presence of TNF alpha + IFN gamma , which act by increasing expression of fg 12 prothrombinase at the feto maternal interface . ^^^ LPS from Escherichia coli and Salmonella enteritidis , or the combination of TNF alpha + IFN gamma , was injected to stimulate abortions . ^^^ The effect of anti MD 1 , which interferes with expression of CD 14 and , hence , with signaling by LPS via the CD 14 tlr4 complex , on TNF alpha + IFN gamma was tested . ^^^ RESULTS : Anti MD 1 completely abrogated TNF alpha + IFN gamma induced abortions . ^^^ CONCLUSIONS : TNF alpha + IFN gamma could not induce murine abortions without co presence of a ' danger ' signal such as LPS acting via CD 14 on toll receptors , and LPS could not act without co signaling by TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , we demonstrated that the productions of TNF alpha , IFN gamma , IL 4 , and IL 10 by Con A stimulated splenocytes from mice at 15 days of infection were inhibited by treatment with salicylate or meloxicam . ^^^ Indomethacin inhibited only TNF alpha and IFN gamma production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1 and tumor necrosis factor ( TNF ) enhance the serine phosphorylation of Stat 1 that occurs in response to interferon gamma ( IFN gamma ) and potentiate IFN gamma mediated , Stat 1 driven gene expression , thus contributing to the synergistic activities of these proinflammatory cytokines . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma , tumor necrosis factor ( TNF ) alpha , IL 4 and IL 6 were investigated for their intracellular presence . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Subsequently , the virus was eliminated from some of the mice , depending on the appearance of functional virus specific CD 8 ( + ) T cells , which have the ability to produce IFN gamma and tumor necrosis factor alpha ( TNF alpha ) and have cytotoxic activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Induction of nitric oxide synthase ( NOS ) by soluble glucocorticoid induced tumor necrosis factor receptor ( sGITR ) is modulated by IFN gamma in murine macrophage . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OxLDL can promote immune activation as determined by enhanced secretion of IFN gamma and TNF by immune competent cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The pathogenetic sequence includes rickettsial entry into the dermis , hematogenous dissemination to vascular endothelial cells ( most critically in brain and lungs ) , increased vascular permeability , edema , and immunity mediated by NK cells , IFN gamma , TNF alpha , RANTES , antibodies , and cytotoxic T lymphocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Additionally , only mild inflammatory cell infiltration and proinflammatory cytokine , interferon gamma ( IFN gamma ) and tumour necrosis factor ( TNF alpha ) production as well as devoid demyelination were seen in facial nerves of the EAN rats . ^^^ On the contrary , severe inflammation and demyelination as well as upregulated IFN gamma and TNF alpha production were observed in sciatic nerves of the same EAN rats . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mitogen stimulated IL 2 , IFN gamma , TNF alpha ( type 1 cytokines ) , and IL 10 ( type 2 cytokine ) production by peripheral blood mononuclear cells , as well as expression of surface markers on immune cells , was evaluated in systemic sclerosis ( SSc ) patients . ^^^ Results showed that ( 1 ) IL 2 production is significantly decreased , whereas IL 10 is higher in untreated patients compared to HCs ; IL 10 , IFN gamma , and TNF alpha production is higher in lSSc compared to dSSc patients ; ( 2 ) CD4+25+ ( IL 2R ) , CD8+ , and CD8+45RA 28+57 ( memory ) lymphocytes are reduced in patients compared to HCs ; ( 3 ) CD 95 expressing CD4+ and CD8+ cells are significantly higher in dSSc patients ; and ( 4 ) steroids are more effective alone than in combination with cyclophosphamide in reducing IL 10 and IFN gamma production in these patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Unlike exposure to the native toxin , the vaccine did not trigger the release of inflammatory cytokines ( TNF alpha , IL 6 , or IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The antiviral cytokines IFN gamma and TNF alpha , and the reactive nitrogen intermediate nitric oxide ( NO ) , were also detected in BAL from 10 infected mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : IL 1 alpha , IL 6 , TNF alpha and IFN gamma were assayed from peripheral and central vein blood at different times . ^^^ RESULTS : We found a significant increase of the IL 6 levels in the supra hepatic samples during liver ischaemia , while the trend with IL 1alpha was less clear ; IFN gamma and TNF alpha were undetectable with the methods used . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To explore the mechanisms underlying such age associated differences in disease outcome , we analysed the in vitro replication of T . cruzi , nitric oxide and tumour necrosis factor alpha ( TNF alpha ) production in peritoneal macrophages ( PMs ) , the serum concentrations of the specific immunoglobulins ( Igs ) IgM and IgG , antibodies exhibiting lytic activity against bloodstream forms of T . cruzi and circulating levels of nitrate , TNF alpha and interferon gamma ( IFN gamma ) . ^^^ When stimulated with IFN gamma , culture supernatants from young PMs contained higher amounts of nitrite and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , in neonatal cells , inactivated virus invoked large levels of the innate immune cytokines IL 6 , TNF alpha , and IL 10 and reduced levels of IFN gamma and IL 12 but no adaptive cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CD 4 and CD 8 T cells induced IL 18 from DC and primed for IL 12 p 70 secretion via interferon gamma ( IFN gamma ) or tumour necrosis factor alpha ( TNF alpha ) . ^^^ However CD 8 T cells , but not CD 4 cells , released IFN gamma / TNF alpha after primary stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neither IL 4 , IL 13 , IL 1beta , IFN gamma , nor TNF alpha alone stimulated TARC release into the supernatant of cultured HASM cells . ^^^ However , both IL 4 and IL 13 increased TARC protein and mRNA expression when administered in combination with TNF alpha but not IL 1beta or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Type 1 polarized Valpha 24 ( + ) Vbeta 11 ( + ) NKT cells produced high levels of IFN gamma , tumor necrosis factor alpha , and granulocyte macrophage colony stimulating factor , and induced strong cytotoxicity in Jurkat cells in an alpha galactosylceramide dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Specifically , IP 10 inhibition in IL 10 ( / ) mice attenuates the associated increases in serum and / or local amyloid A , IL 2 , IL 6 , TNF alpha , IFN gamma , IL 1alpha , and IL 1beta with colitis as compared with IL 10 ( / ) mice that develop colitis similar to human Crohn ' s disease . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study we show that in vitro exposure of naive human PBMC to Leptospira interrogans results in cell proliferation and the production of IFN gamma , IL 12 , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of macrophages with IFN gamma followed by GIP sVSG ( the soluble form of VSG containing the glycosylinositolphosphate substituent that is released by parasites ) stimulated the induction of gene expression , including transcription of TNF alpha , IL 6 , GM CSF , and IL 12p40 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Exposure of RAW264 . 7 cells to apoptotic CTLL 2 cells induced both SLPI and TNF alpha , and addition of IFN gamma inhibited SLPI but augmented TNF alpha production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , RNA was obtained from pleural cells and examined for TGF beta 1 , tumor necrosis factor alpha ( TNF alpha ) , IFN gamma , and interleukin 8 ( IL 8 ) expression by real time PCR . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It was found that stimulation of the HMEC 1 microvascular endothelial cell line with the pro inflammatory cytokines IFN gamma and TNF alpha caused a transient decrease in the level of NDST 1 and 2 transcripts after 4 hours ( P < 0 . 05 and P < 0 . 01 respectively ) , but the expression of NDST 1 increased above control levels after 16 hours ( P < 0 . 01 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokines IFN gamma and TNF alpha and several chemoatractants molecules , which act on G protein coupled serpentine receptors , are produced during the acute infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukins ( IL ) 1 beta , 8 , 18 , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) were quantified by ELISA in plasma and washes of a terminal part of the small bowel . ^^^ In the gut of non infected piglets , IL 1 beta , IL 8 and IL 18 were detected whereas TNF alpha and IFN gamma were mostly missing . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Demographic and health behavior variables , quality of life ( EORTC QLQ C 30 ) , mood ( POMS ) , stress ( SOSI ) , and counts of NK , NKT , B , T total , T helper , and T cytotoxic cells , as well as NK and T cell production of TNF , IFN gamma , IL 4 , and IL 10 were assessed pre and postintervention . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Polyphenon inhibited the lethal toxicity of SEB and the SEB induced production of TNF alpha , IFN gamma and IL 4 following its intraperitoneal administration to BALB / c mice . ^^^ Furthermore , EGCg inhibited SEB induced TNF alpha and IFN gamma production and IL 2 , IFN gamma , IL 10 and IL 12 p 40 mRNA expression in human PBMCs from normal donors in a dose dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The anti TNF alpha mAb did not reduce the iNOS expression in IFN gamma stimulated L . major infected neutrophils in vitro . ^^^ Anti TNF alpha mAb treatment caused an increase in the production of IFN gamma and IL 10 by the lymph node cells from infected mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In particular , substantial amounts of TNF alpha , IL 6 , CCL 2 and CXCL 10 , in the absence of IFN alpha , IFN gamma , IL 15 , IL 12p70 and CX3CL1 , can be measured . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effect is increased further when IFN gamma is combined with TNF , lipopolysaccharide ( LPS ) , or dsRNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Thus , T cell mechanisms exist that control LVS intracellular growth without acting through the IFN gamma receptor ; such control is due in large part to TNF alpha , and is partially mediated by a unique double negative T cell subpopulation . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neutralizing antibodies against human RANTES , MIP 1alpha , MIP 1beta , alpha interferon ( IFN alpha ) , IFN beta , IFN gamma , interleukin 4 ( IL 4 ) , IL 10 , IL 13 , IL 16 , MCP 1 , MCP 3 , tumor necrosis factor alpha ( TNF alpha ) , or TNF beta failed to reverse the HIV 1 suppressive activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Monocyte derived DCs of HIGM 3 subjects on ex vivo stimulation with tumor necrosis factor alpha ( TNF alpha ) or lipopolysaccharide ( LPS ) combined with interferon gamma ( IFN gamma ) normally express all the markers of mature DCs , such as CD 83 and DC LAMP . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Incubating Calu 3 bronchiolar epithelial monolayers with cytomix ( a mixture of 1 , 000 U / ml IFN gamma , 10 ng / ml TNF alpha , and 1 ng / ml IL 1beta ) increased permeability to FD 4 , but adding l NIL prevented this effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this report of a patient undergoing allogeneic SCT for AML , serial cytokine measurements by real time PCR revealed increased production of interferon gamma ( IFN gamma ) and TNF alpha , but not interleukin ( IL ) 4 in purified T cells following withdrawal of immunosuppression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Costimulation of HIV 1 transgenic mice spleen cells with LPS and STF or CpG DNA induced TNF alpha and IFN gamma production in a synergistic manner and p 24 production in an additive fashion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Importantly , human hepatic Valpha 24 ( + ) T cells are potent producers of IFN gamma and TNF alpha , but not IL 2 or IL 4 , when stimulated pharmacologically or with the NKT cell ligand , alpha galactosylceramide . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The production of IFN gamma , TNF alpha , and IL 1beta by LPS stimulated spleen cells was decreased , and IL 10 was increased , in cells from Crry Tg mice in comparison to WT . ^^^ The steady state mRNA levels for IFN gamma , TNF alpha , and IL 1beta were all decreased in the joints of Crry Tg mice in comparison to WT . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Ex vivo analyses revealed that in both cases , the effects were associated with inhibition of collagen specific pro inflammatory / Th1 cytokine ( TNF alpha , IL 6 , and IFN gamma ) release . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After mice were killed on d 28 , serum IFN gamma level was measured by ELISA , and pancreatic IL 1beta and TNF alpha mRNA expression was detected by semi quantitative reverse transcription PCR ( RT PCR ) . ^^^ In IL 10 treated mice pancreatic IL 1beta and TNF alpha mRNA expression was depressed , and serum IFN gamma concentration and the number of spleen CD4+ or CD8+ lymphocytes were decreased on d 28 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , 5 proinflammatory cytokines ( IL 1beta , IL 6 , TNF alpha , IFN gamma and IL 1 receptor antagonist , IL 1ra ) present in colostrum were tested for their potential to enhance mitogenic response and to elicit expression of IL 2 mRNA and CD 25 in peripheral blood mononuclear cells ( PBMC ) from newborn calves before being fed colostrum . ^^^ Pretreatment of PBMC from newborn calves with IL 1beta , TNF alpha or IFN gamma significantly enhanced the ConA response , whereas IL 1ra inhibited the response . ^^^ Although IL 2 mRNA expression in ConA stimulated PBMC from newborn calves was weaker than that in those from adult cows of ConA stimulated controls , the expression levels became comparable after pretreatment with IL 1beta , TNF alpha or IFN gamma . ^^^ These results suggest that pretreatment of neonatal PBMC with IL 1beta , TNF alpha or IFN gamma promotes mitogenic response to ConA through up regulating the production of IL 2 and the expression of the mature IL 2 receptor . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study compares Eta and Ifx in vitro regarding transmembrane tumour necrosis factor alpha ( tmb TNF alpha ) expression and interferon gamma ( IFN gamma ) , TNF alpha and granulocyte macrophage colony stimulating factor ( GM CSF ) production in intestinal T lymphocytes . ^^^ CONCLUSIONS : We have established that Eta is just as efficient as Ifx in downregulating IFN gamma and GM CSF production in vitro and Eta bound to tmb TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In order to investigate these effects on the canine immune system and to elucidate possible mechanisms we determined the following immune parameters in the blood of 33 clinically sound German shepherd dogs before and after standard vaccination with a polyvalent vaccine against distemper , parvovirus , viral hepatitis , leptospirosis , kennel cough and rabies : white and differential blood cell count , the serum concentrations and / or activities of IL 1 , IL 2 , IFN gamma , TNF alpha , neopterin and IgG , natural killer ( NK ) cell activity , bactericidal activity and complement hemolytic activity , lymphocyte proliferation test ( LPT ) and nitroblue tetrazolium test ( NBT ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We used sequence specific primers ( SSP ) polymerase chain reaction to study interleukin 10 ( IL 10 ) polymorphisms at 1082 ( G / A ) , 819 ( C / T ) , and 592 ( C / A ) ; tumor necrosis factor alpha ( TNF alpha ) at 308 ( G / A ) and 238 ( G / A ) ; transforming growth factor beta ( TGF beta ) variants at codon 10 ( C / T ) and codon 25 ( G / C ) ; and interferon gamma ( IFN gamma ) polymorphisms at +874 ( T / A ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokines TNF alpha , IFN gamma , IL 1 beta , and IL 4 stimulated a 2 to 3 . 5 fold increase in CD 44 dependent cell binding to HA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Secretion of proinflammatory cytokines IL 1 beta , TNF alpha , IL 6 , IFN alpha , and IFN gamma by the chorion tissue ( 8 12 weeks ) was studied in normal gestation and spontaneous abortion . ^^^ The production of IL 1 beta , TNF alpha , and IFN alpha virtually did not change in spontaneous abortion , while IFN gamma was not secreted in all experimental groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , MS patients in remission produced significantly less spontaneous and MBP induced TNF alpha , spontaneous , PHA and MBP induced IL 1beta and PHA induced IFN gamma together with increased production of biologically active TGF beta 1 . ^^^ We conclude that the balance between biologically active TGF beta 1 and the pro inflammatory TNF alpha , IL 1beta and IFN gamma is dysregulated during MS relapse remission and that normal counter regulatory mechanisms during the relapse phase are defective . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cerebral malaria is a severe complication of infection with Plasmodium berghei ANKA involving the Th 1 cytokines TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Eg2HSP70 stimulated PBMC from 26 patients produced significantly greater amounts of TNF alpha , IFN gamma , and IL 10 than unstimulated cultures in all patients , irrespective of the presence of allergic reactions ( P < 0 . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We now report that tumor necrosis factor alpha ( TNF alpha ) , an important cytokine for intracellular killing of mycobacteria , remains deficient in these C3H / HeJ mice compared to C3H / OuJ mice even at a high concentration of ara LAM with either IFN gamma or IFN beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although IL 6 , IL 10 , IL 18 , and TNF alpha were produced in the heart during acute infection , BALB / c mice lacked a substantial IL 2 and IFN gamma response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Peripheral blood mononuclear cells ( PBMCs ) were cultured in the presence or in the absence of 10 microg / ml MDI HSA ( 4 , 4 ' diphenylmethane diisocyanate ) HSA ( human serum albumin ) . ( 3 ) H thymidine uptake , mRNA expression by RT PCR ( beta actin , IL 1beta , IL 2R , IL 4 , IL 5 , IL 6 , IL 10 , IFN gamma , TNF alpha , TGF beta ) were estimated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha synergized with IL 4 , and to a lesser extent with IFN gamma and IL 13 , to release RANTES , IL 8 and gro alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro primed T cells ( 2 4 cycles of stimulation with Ag and DC ) showed significant antigen specific cytokine responses ( IFN gamma , TNF alpha , GM CSF ) to TT . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
No local or systemic increases in TNF alpha , IL 1beta or IFN gamma were observed during infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
BPH tissue derived T cells ( n = 10 ) were isolated , activated ( PMA + ionomycin ) , and analyzed for intracellular reactivity with anti IFN gamma and IL 2 , 4 , 5 , 6 , 10 , and 13 , as well as TNF alpha and beta by four color flow cytometry . ^^^ Lymphokine release upon stimulation was found in the case of IL 2 , IL 5 , IFN gamma , and TNF alpha > 4 microg ; of IL 4 > 2 microg ; and of IL 10 > 1 microg / ml . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To gain further insight into the cytokine production profile in response to measles vaccination , we studied interferon gamma ( IFN gamma ) , tumor necrosis factor ( TNF alpha ) , soluble IL 2 receptor ( sIL 2R ) , interleukin 2 ( IL 2 ) , interleukin 4 ( IL 4 ) , and interleukin 6 ( IL 6 ) in both supernatants from peripheral blood mononuclear cells ( PBMC ) stimulated with phytohaemagglutinin ( PHA ) , and plasma . ^^^ However , plasma levels of Th 1 cytokines ( IFN gamma , sIL 2R and TNF alpha ) were preferentially activated by measles virus after the first dose of measles vaccination . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Significant cytotoxic / cytostatic activity was elicited by treating ascitic MO / MA with interferon gamma ( IFN gamma ) and interleukin 2 ( IL 2 ) , but not with interleukin 12 , paclitaxel , granulocyte monocyte colony stimulating factor ( GM CSF ) , or tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum TNF alpha , IL 3 , and IFN gamma levels were not significantly different in all studied groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
At the same time , the gene and protein expressions of tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) were also markedly upregulated in various tissues . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Colonization rates of H . pylori , degree of gastric inflammation and other pathological changes including atrophic gastritis and metaplasia , serum levels of IL 1beta , TNF alpha , IFN gamma and IL 10 , mRNA transcripts of various mouse cytokines and chemokines , and NF kappaB binding activities , and finally the presence of gastric adenocarcinoma were compared between an H . pylori infected group ( HP ) , and an H . pylori infected group administered with long term rebamipide containing pellet diets ( HPR ) . ^^^ RESULTS : Serum levels of IL 1beta , IFN gamma and TNF alpha , the gastric mucosal expression of ICAM 1 , HCAM and MMP , and transcriptional regulation of NF kappaB DNA binding were all significantly decreased in the HPR group compared with the HP group . ^^^ An RNase protection assay showed , in the rebamipide administered group , significantly decreased mRNA levels of apoptosis related genes such as caspase 8 , FasL , Fas , TRAIL and various cytokines genes such as IFN gamma , RANTES , TNF alpha , TNFR p 75 , IL 1beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Previously , we produced H 2L ( d ) restricted CTL reactive against the CMS 4 sarcoma , which expresses a naturally occurring rejection Ag recognized by these CTL and harbors a cytokine ( IFN gamma plus TNF ) inducible , Fas responsive phenotype . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The combination of apoptotic cells and TLR ligands enhanced early secretion of TNF alpha , MIP 1alpha , and MIP 2 and increased late TGF beta 1 secretion , while suppressing late TNF alpha , IL 10 , and Il 12 by mechanisms which could nevertheless be overridden by IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Levels of interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin ( IL ) 1beta , IL 6 , IL 8 , IL 10 and IL 12 were assayed before Foscan administration ; after anesthetic induction , surgical resection and light delivery ; in postoperative recovery and the day after the surgery . ^^^ IFN gamma , TNF alpha and IL 12 showed no elevation , but IL 1beta , IL 6 , IL 8 and IL 10 levels were elevated after surgery and PDT . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Marked elevation of serum levels of cytokines such as TNF alpha or IFN gamma suggests that these cytokines played an important role in the pathogenesis of the hepatic cell necrosis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Intracellular cytokine stainings for proinflamatory cytokine ( TNF alpha ) , type 1 cytokines ( IFN gamma and IL 2 ) and type 2 cytokines ( IL 4 and IL 5 ) in CD4+ and CD8+ T lymphocytes were determined by flow cytometer . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , elevated production of liver associated proinflammatory cytokines ( interleukin 12 , tumor necrosis factor alpha [ TNF alpha [ , and gamma interferon [ IFN gamma ] ) and chemokines ( MIP 1 alpha , MIP 2 , and MCP 1 ) was observed within the first 24 h of infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MOG specific gamma interferon ( IFN gamma ) , nitric oxide , and tumor necrosis factor alpha production by splenocytes was significantly reduced in schistosome infected mice compared to uninfected mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Resistance to infection is associated with the development of CD 4 ( + ) Th 1 cells producing gamma interferon ( IFN gamma ) and tumor necrosis factor ( TNF ) , which synergize in activating macrophages to their microbicidal state . ^^^ Thus , IFN gamma , FasL , and iNOS appear to be essential for the complete control of parasite replication , while the contribution of TNF is more important in controlling inflammation at the site of parasite inoculation . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The aim of this study was to investigate the frequency of genotypes associated with polymorphisms of TNF alpha , TGF beta , IL 10 , IL 6 , and IFN gamma and to determine their association with the outcome of HCV infection . ^^^ All patients were genotyped as high or low producers of TNF alpha and IL 6 and high , intermediate , or low producers of TGF beta , IL 10 , and IFN gamma based on single nucleotide substitutions . ^^^ No associations were observed between polymorphisms of TNF alpha , IL 10 , or IFN gamma and viral clearance or persistent infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The proinflammatory cytokines interleukin beta ( IL 1beta ) , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma [ see symbol in text ] ) , and interleukin 12 ( IL 12 ) were induced by viral infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : The effects on expression of HLA DR and costimulatory molecules ( CD 80 , CD 86 , ICAM 1 , and CD 40 ) on normal conjunctival epithelial cells and ChWK treated with various cytokines ( IFN gamma , TNF alpha , IL 4 , and IL 13 ) were determined using flow cytometry and confocal microscopy . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 10 ( / ) mice that survive a primary infection have been shown to control gamma interferon ( IFN gamma ) and TNF alpha production , indicating that other cytokines or mechanisms may be involved in their down regulation . ^^^ Significantly higher levels of transforming growth factor beta ( TGF beta ) , a cytokine with such properties , are present in the plasma of infected IL 10 ( / ) mice at a time that coincides with the disappearance of IFN gamma and TNF alpha from the blood . ^^^ Neutralization of TGF beta in IL 10 ( / ) mice resulted in higher circulating amounts of TNF alpha and IFN gamma , and all treated IL 10 ( / ) mice died within 12 days with increased pathology but with no obvious increase in parasitemia . ^^^ Our data suggest that a tight regulation of the balance between regulatory cytokines such as IL 10 and TGF beta and inflammatory cytokines such as IFN gamma and TNF alpha is critical for survival in a mouse malaria infection . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , stimulation with a proinflammatory cytokine cocktail containing IFN gamma , TNF alpha , and IL 1beta , caused induction of NK 1R expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Injection of lipoplexes containing plasmid DNA , regardless of IFN gene insertion , stimulated a transient increase in the serum concentration of proinflammatory cytokines such as tumor necrosis factor ( TNF ) alpha and IFN gamma , while injection of lipoplexes containing pI : pC led to a low level of TNF alpha and undetectable IFN gamma production . ^^^ In tumor prophylactic experiments , intravenous injection of lipoplexes containing plasmid , regardless of IFN gene insertion , showed a significant reduction in lung metastatic nodules probably due to proinflammatory cytokines such as TNF alpha and IFN gamma nonspecifically induced by the CpG motifs in the plasmid and the type 1 IFNs produced . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The K 2 specific lymphocyte proliferation and mRNA expressions of Th 1 type cytokines ( IL 12p40 , IFN gamma and TNF alpha ) in draining lymph node and inflamed eyes were reduced in TRD treated mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Additionally , IFN gamma and TNF alpha production and nuclear translocation of NF kappaB was enhanced . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To obtain basic information on the state of proinflammatory cytokines in newborn calves , we determined the kinetics of 5 cytokines ( IL 1beta , IL 6 , TNF alpha , IFN gamma and IL 1 receptor antagonist ) in sera of newborns during the first 4 weeks of life . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
LPMCs from DSS fed IL 18 ( / ) mice produced significantly higher amounts of IFN gamma , while LPMCs from DSS fed IL 12 ( / ) mice produced lower amounts of IFN gamma and tumour necrosis factor ( TNF ) alpha compared with control mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We studied the frequency of interleukin 2 ( IL 2 ) , gamma interferon ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) and IL 10 expressing CD3+ cells in 10 non immune malaria patients with uncomplicated malaria and in one patient with cerebral malaria after P . falciparum specific and non specific mitogenic stimulation . ^^^ CD3+ cells of patients responded to P . falciparum infected erythrocytes with significant increases in the percentage of IL 2 , IFN gamma , and TNF alpha , but also IL 10 , positive cells . ^^^ The results indicate that the increased responsiveness of in vivo primed compared to malaria nave CD3+ cells is Plasmodium specific and biased towards production of IFN gamma and TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Instead , the densities of IL 2 , gamma interferon ( IFN gamma ) , and tumor necrosis factor alpha positive cells , the density of IFN gamma mRNA positive cells , and the amounts of IFN gamma mRNA detected by RT PCR correlated with the degree of celiac disease in patients with type 1 diabetes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interestingly , TNF alpha addition to IFN gamma reverted the Stx resistant phenotype , which corresponded with its well established enhancing effect on Stx toxicity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : A two color flow cytometric technique was used to measure interleukin ( IL ) 1beta , IL 6 , tumor necrosis factor alpha ( TNF alpha ) , IL 10 , and IL 12 production blocked intracellularly with brefeldin A in lipopolysaccharide ( LPS ) stimulated CD 14 ( + ) monocytes and IL 2 , IL 4 , and IFN gamma production in phorbol 12 mirystate 13 acetate ( PMA ) stimulated CD 3 ( + ) T lymphocytes in samples from patients with rheumatoid arthritis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In situ expression of CD 40 , CD40L ( CD 154 ) , IL 12 , TNF alpha , IFN gamma and TGF beta 1 in murine lungs during slowly progressive primary tuberculosis . ^^^ The distribution and expression of CD 40 , its ligand CD40L ( 154 ) and related cytokines interleukin 12 ( IL 12 ) , tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) and transforming growth factor beta 1 ( TGF beta 1 ) were studied in the lungs of B6D2F1 hybrid mice during slowly progressive primary tuberculosis ( TB ) by immunohistochemistry . ^^^ Ags ) , CD40L and IFN gamma expression were maintained throughout infection , and TNF alpha expressing cells were decreased . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : We found that induction of CD 59 up regulation required specific binding of the lectin to terminal alphaGal and was not induced either by soluble factors that may be released from EC by stimulation with the lectin or by TNF alpha , IFN gamma , or IL 1alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NFAT regulated genes exhibited lower expression in UCB CD4+ T cells including the following : granulocyte macrophage colony stimulating factor ( GM CSF ) , interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 3 ( IL 3 ) , IL 4 , IL 5 , IL 13 , IL 2 receptor alpha ( IL 2Ralpha ; CD 25 ) , CD40L , and macrophage inflammatory protein 1 alpha ( MIP 1alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Isolated islets from wild type ( WT ) or IRF 1 ( / ) C57BL / 6 mice were cultured in a mixture of IL 1beta , TNF alpha , and IFN gamma + / the iNOS inhibitor L NMMA . ^^^ CONCLUSIONS : IFN gamma , IL 1beta , and TNF alpha in combination induce chemokine gene expression in pancreatic islets . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Fve , a major fruiting body protein from Flammulina velutipes , a mushroom possessing immunomodulatory activity , stimulates lymphocyte mitogenesis , suppresses systemic anaphylaxis reactions and edema , enhances transcription of IL 2 , IFN gamma and TNF alpha , and hemagglutinates red blood cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study , we show that in human PBMC resistin mRNA expression determined by fluorescence based real time polymerase chain reaction is strongly increased by the proinflammatory cytokines interleukin ( IL ) 1 , IL 6 , tumor necrosis factor alpha ( TNF alpha ) , and also by lipopolysaccharides ( LPS ) , respectively , while no effect was found by interferon gamma ( IFN gamma ) or leptin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Influence of pro inflammatory ( IL 1 alpha , IL 6 , TNF alpha , IFN gamma ) and anti inflammatory ( IL 4 ) cytokines on chondrocyte function . ^^^ To investigate the influence of pro inflammatory cytokines ( IL 1 alpha , IL 6 , TNF alpha , IFN gamma ) and subsequently the possible beneficial role of an anti inflammatory cytokine ( IL 4 ) on chondrocyte viability ( necrosis / apoptosis ) , proliferation and nitric oxide ( NO ) production . ^^^ Cells were incubated with cytokines ( IL 1 alpha , IFN gamma , TNF alpha , IL 4 , IL 6 ) at 0 . 1 , 1 , 10 and 100 ng / mL . ^^^ RESULTS : The viability and proliferation of bovine chondrocytes decreased after incubation with 100 ng / mL IL 1 alpha , TNF alpha or IFN gamma . ^^^ IFN gamma and TNF alpha induced NO production only at the highest concentration ( 100 ng / mL ) , whereas IL 4 and IL 6 did not . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Type 1 ( IFN gamma IL 2 and TNF alpha ) and type 2 ( IL 4 , IL 6 and IL 10 ) cytokines were determined in three CD8+ T subsets , i . e . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We measured the production of interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF ) and interleukin 10 ( IL 10 ) in peripheral blood lymphocytes by flowcytometry after stimulation without and with IgG in different doses ( 1 , 5 and 10 mg / ml ) . ^^^ RESULTS : IFN gamma and TNF were decreased significantly ( P = 0 . 001 ) in the untreated and interferon beta ( IFN beta ) treated patients after stimulation with IgG . ^^^ The reduction of the pro inflammatory cytokines IFN gamma and TNF after stimulation with different IgG doses was clearly dose dependent in all groups . ^^^ CONCLUSION : Besides a suppression of the pro inflammatory cytokines IFN gamma and TNF , IgG enhances the anti inflammatory cytokine IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , oral administration of ANG did not cause licking , scratching and biting responses induced by TNF alpha ( 100 pg ) , IFN gamma ( 100 pg ) or IL 1beta ( 100 pg ) injected intrathecally ( i . t . ) , especially at higher dose ( 3 g / kg ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) are critical in the development of an effective immune response . ^^^ Ten weeks after primary vaccination , cells from vaccinated calves and adults , and nonvaccinated , age matched calves ( n = 4 ) were evaluated in vitro for their capacity to produce IFN gamma and TNF alpha . ^^^ IFN gamma and TNF alpha concentrations in culture supernatants harvested at these times were quantified by enzyme linked immunosorbent assay ( ELISA ) . ^^^ PPD induced IFN gamma and TNF alpha responses of cells from vaccinated calves and adults were greater than responses of autologous unstimulated cells . ^^^ Incubation period also influenced the magnitude of both IFN gamma and TNF alpha , responses in PPD and PWM stimulated cultures . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , CD 4 CD 8 NKT cells , that mainly secrete TNF alpha and IFN gamma , are reduced in number and attenuated in cytokine secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines such as IFN gamma , IL 3 and TNF alpha induce MHC antigen expression on neural cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : The effect of inhibitory cytokines ( tumor necrosis factor alpha [ TNF alpha ] , interferon gamma [ IFN gamma ] , macrophage inflammatory protein 1 alpha [ MIP 1alpha ] , and transforming growth factor beta 1 [ TGF beta 1 ] ) was investigated , using clonogenic assays , competitive repopulation , and in vivo induction of proinflammatory cytokines by double stranded RNA . ^^^ RESULTS : TNF alpha , IFN gamma , MIP 1alpha , and TGF beta 1 suppressed colony formation in a dose dependent fashion that was similar for PIGA+ and PIGA blood bone marrow cells . ^^^ Competitive repopulation of bone marrow cells cultured in IFN gamma and TNF alpha resulted in a comparable ability of PIGA+ and PIGA hematopoietic stem cells to reconstitute hematopoiesis . ^^^ Fas expression was minimal on PIGA+ and PIGA progenitor cells and was up regulated to the same extent in response to IFN gamma and TNF alpha as assessed by Fas antibody mediated apoptosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To understand the pathogenesis of vaccine modified measles ( VMM ) , we measured plasma levels of IFN gamma and IL 2 ( Th 1 cytokines ) , IL 4 and IL 10 ( Th 2 cytokines ) , IL 12 , TNF alpha and TGF beta 1 in children with uncomplicated measles , who had anti measles IgG antibodies and with a history of immunization on admission ( day 0 ) , day 14 and day 60 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
By administration of anti CD 3 Abs , we demonstrated that this early response was linked to the marked T lymphocyte lung infiltration and to the overproduction of the proinflammatory mediators such as TNF alpha , IFN gamma , and NO in IL 4 ( / ) mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The 5 / 4E8 hybridoma showed greater flexibility in recognition , including a higher responsiveness to the corresponding self ( mouse ) aggrecan peptide , and produced more inflammatory cytokines ( IFN gamma and TNF alpha ) , whereas hybridoma TA 20 produced IL 5 in response to either human or mouse self peptide stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Most of the mAbs were against beta ( 1 ) and beta ( 2 ) integrins , but one mAb , 6B9 , significantly inhibited T cell TEM across IFN gamma , TNF alpha , and IFN gamma plus TNF alpha stimulated HUVEC , and did not react with an integrin . 6B9 mAb did not inhibit T cell adhesion to HUVEC , suggesting that 6B9 blocked a novel pathway in T cell TEM . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
By real time PCR , our data confirmed that AD , as compared with psoriasis , is associated with elevated skin production of Th 2 cytokines and low levels of proinflammatory cytokines such as TNF alpha , IFN gamma , and IL 1beta . ^^^ We found that IL 13 and IL 4 inhibited TNF alpha and IFN gamma induced HBD 3 production . ^^^ These studies indicate that decreased expression of a constellation of antimicrobial genes occurs as the result of local up regulation of Th 2 cytokines and the lack of elevated amounts of TNF alpha and IFN gamma under inflammatory conditions in AD skin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Therefore , we compared the effects of human recombinant IL 1alpha , IL 1beta , IL 6 , IFN gamma , and TNF alpha individually or in combination , on HepG 2 secretion of complement component C 3 , the major opsonic protein of the complement system . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Thymus and activation regulated chemokine ( TARC / CCL17 ) produced by mouse epidermal Langerhans cells is upregulated by TNF alpha and IL 4 and downregulated by IFN gamma . ^^^ TARC production by murine LC was upregulated by TNF alpha and IL 4 and downregulated by IFN gamma , dose dependently . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro studies showed that anti FasL MAb did not inhibit IFN gamma production by anti CD3 / CD28 stimulated lamina propria CD4+ T cells but suppressed TNF alpha and IL 1beta production by lamina propria mononuclear cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma was the highest induced cytokine followed by IL 10 , TNF alpha and lastly IL 4 . ^^^ Also , abrogation of induction of the proinflammatory cytokines IFN gamma and TNF alpha but not the antiinflammatory cytokines IL 4 and IL 10 by sonicated and filtrated bacteria was depicted . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Levels of TNF alpha , IL 1beta , IL 6 , IL 10 , IL 18 , IFN gamma , MCP 1 , and PCT ( procalcitonin ) were measured by ELISA . ^^^ In urine , in contrast with TNF alpha , IL 1beta , IL 6 , IL 10 , IFN gamma , and MCP 1 in which no differences between the two groups were observed , a distinct trend of elevated IL 18 levels was observed only in the septic group . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To evaluate in vitro indices of cellular sensitization , antigen induced gamma interferon ( IFN gamma ) , nitric oxide ( NO ) , and tumor necrosis factor alpha ( TNF alpha ) responses by blood mononuclear cells from Mycobacterium bovis infected cattle were quantified and compared . ^^^ Using an aerosol model of infection , two doses of each of two strains of M . bovis ( 95 1315 and HC 2045T ) were used to induce a range of IFN gamma , NO , and TNF alpha responses . ^^^ Infection specific increases in NO , but not in IFN gamma or TNF alpha , were detected in nonstimulated cultures at 48 h , a finding that is indicative of nonspecific activation and spontaneous release of NO . ^^^ At 34 days postinfection , IFN gamma , NO , and TNF alpha responses in antigen stimulated cells from cattle receiving 10 ( 5 ) CFU of M . bovis organisms were greater than responses of cells from cattle infected with 10 ( 3 ) CFU of M . bovis organisms . ^^^ The NO response , but not the IFN gamma and TNF alpha responses , was influenced by infective strains of M . bovis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The production of high levels of interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) by antigen specific T cells was found in six patients ( 46 . 0 % ) who showed significantly lower HBV DNA levels in serum at 6 ( P = 0 . 04 ) and 18 months ( P = 0 . 005 ) after completion of therapy than those without high levels of cytokine production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Real time RT PCR was performed using fluorescent probes and primers for cytokines including IL 1 beta , IL 2 , IL 4 , IL 5 , IL 8 , IL 10 , IFN gamma , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Integrated measurements by flow cytometry of the cytokines IL 2 , IFN gamma , IL 12 , TNF alpha and functional evaluation of their receptors in human blood . ^^^ We have measured by flow cytometry , within 2 days , not only the production of IL 2 , IFN gamma , IL 12 and TNF alpha , but also the functional expression of the receptors for these cytokines in blood samples obtained from 15 healthy donors and 13 patients suffering from tuberculosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The results showed : ( 1 ) six cytokines , MIP 1alpha , MIP 2 , TGF beta , TNF alpha , IFN gamma and Tbeta ( 4 ) , inhibited the growth of CFU GM when murine WEHI 3 conditioned medium was added to the culture system as a source of colony stimulation . ( 2 ) The original endothelial cell conditioned medium ( E CM ) did not affect the production of CFU GM , but the > 10 kD component of E CM increased its production , and the < 10 kD component decreased it . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Analysis of cytokine messages showed increased expression of IL 12 , IFN gamma and TNF alpha , but not IL 4 mRNAs in spleen cells after infection with the T . gondii strains RH and Beverley . ^^^ High levels of circulating IL 12 and IFN gamma were detected in the serum of mice infected with strain RH , although TNF alpha levels remained low . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cutaneous and systemic overexpression of several proinflammatory cytokines , particularly type 1 cytokines such as IL 2 , IL 6 , IL 8 , IL 12 , IFN gamma and TNF alpha , has been demonstrated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Analysis of mRNA expression of cytokines in the spleen revealed a significant decrease in the levels of TNF alpha , TGF beta , IL 12 and IFN gamma mRNA expression , which usually precedes cytokine protein expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 2 , IFN gamma and TNF alpha mRNA levels were decreased in colonic T lymphocytes , and virtually no IL 2 , IFN gamma , TNF alpha or TGF beta positive cells were detected in basal lymphoid aggregates . ^^^ Lamina propria outside the aggregates contained IL 10 , IFN gamma , TNF alpha and TGF beta but not IL 2 positive cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , in the presence of MBP , MOG peptides or alpha beta crystallin , a significant percent age of lymphocytes from MS patients underwent blast transformation , which resulted in high levels of interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) and tumour necrosis factor beta ( TNF beta ) production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines including TNF alpha , IFN gamma , IL 6 , and IL 8 were measured with ELISA , and T subgroups were detected with alkaline phosphatase anti alkaline phosphatase ( APAAP ) technique . ^^^ The levels of cytokines in patients with severe hepatitis B were higher ( TNF alpha 359 . 0+ / 17 . 2 ng / L , IFN gamma 234 . 7+ / 16 . 5 ng / L , IL 6 347 . 5+ / 31 . 3 ng / L , IL 8 181 . 1+ / 19 . 6 ng / L ) than those in acute hepatitis B ( TNF alpha 220 . 6+ / 8 . 9 ng / L , IFN gamma 174 . 9+ / 12 . 0 ng / L , IL 6 285 . 8+ / 16 . 5 ng / L , IL 8 118 . 4+ / 5 . 1 ng / L , P < 0 . 01 or 0 . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Quantitative rtPCR for IFN gamma , TNFalpha and IL 4 was conducted on grafts at 5 and 9 days post implantation . ^^^ There were also significantly fewer TNFalpha transcripts in allografts of IFN gamma / intestine than in wild type allografts ( P = 0 . 02 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) synergistically enhances the IDO activity induced by IFN gamma at the level of transcription in human epithelial cells . ^^^ The purpose of this study was to characterize the molecular mechanisms responsible for synergistic gene expression in response to IFN gamma and TNF alpha . ^^^ It was found that IFN gamma induced mechanisms , such as the binding of Stat 1 to gamma activation sequences ( GAS ) and IFN regulatory factor 1 ( IRF 1 ) to IFN stimulated response elements ( ISREs ) , are more highly activated following treatment with IFN and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RNA was extracted from intestinal graft and native liver biopsies for cytokine measurements ( IL 1 alpha , IL 1 beta , IL 2 , IL 4 , IL 5 , IL 6 , IFN gamma , TNF alpha , and TNF beta ) . ^^^ Irrespective of the type of donor specific cell augmentation , severe rejection caused strong cytokine expression in the grafts of IL 1 alpha , IL 1 beta , IFN gamma , and TNF alpha ; in the native livers , mainly of TNF alpha ( with IFN gamma showing hardly any increase ) . ^^^ Mild rejection correlated well with strong intragraft expression of IL 6 , TNF alpha , and TNF beta ( early rejection markers ) ; severe rejection with IL 1 alpha , IL 1 beta , IFN gamma , and TNF alpha ( late rejection markers ) . ^^^ With immunosuppression , bone marrow infusion increased intragraft cytokine expression of IL 1 alpha , IL 1 beta , IFN gamma , and TNF alpha , as well as liver cytokine expression of IL 1 beta , compared to blood transfusion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Intracellular staining demonstrated that most intraepidermal CD8+ T cells were capable of producing IFN gamma and TNF alpha but produced little IL 2 and IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Recent animal studies highlighted the importance of type 1 cytokines in protection against Candida , and previous work suggested that CMC patients may exhibit altered cytokine production in response to CANDIDA : Based on these findings , in this study we investigated cytokine production in CMC patients by assessing a range of inflammatory , anti inflammatory , type 1 , and type 2 cytokines ( interleukin 2 [ IL 2 ] , IL 4 , IL 5 , IL 6 , IL 10 , IL 12 , gamma interferon [ IFN gamma ] , tumor necrosis factor alpha [ TNF alpha ] ) in whole blood cultures in response to five different fractions of Candida albicans ( carbohydrate , purified mannan , and protein rich fractions , etc . ) , as well as non Candida antigens . ^^^ Our results demonstrate that cytokine production is deregulated in a Candida specific way for some cytokines ( IL 2 , IL 10 ) , is deregulated more generally for other cytokines ( IL 12 , IL 6 , IFN gamma ) , and is not markedly altered for still other cytokines ( TNF alpha , IL 4 , IL 5 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 2 , IL 12 , IFN gamma , TNF alpha , and TRAIL are implicated in enhancing cytotoxic and apoptotic effects in response to colon adenomas . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Upregulation of secreted MCP 1 was observed following activation with the pro inflammatory cytokines TNF alpha , IL 1 beta and IFN gamma , and was reduced following dexamethasone treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokines IL 5 , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) also predicted development of aGVHD ( P < . 05 for all 3 ) , appearing to be coproduced in the assay and correlating with estimated CTLp frequencies . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mouse splenocyte cultures treated with HSV DNA or HSV derived oligodeoxyribonucleotides ( ODNs ) showed strong proliferative responses and production of inflammatory cytokines ( gamma interferon [ IFN gamma ] , tumor necrosis factor [ TNF ] , and interleukin 6 [ IL 6 ] ) in vitro , whereas splenocytes treated with mammalian CV 1 DNA or non CpG ODN did not . ^^^ Furthermore , HSV ODN synergized with IFN gamma to induce nitric oxide ( NO ) , IL 6 , and TNF production from macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
As compared with controls , we found major changes in T lymphocyte subsets in HCV infected patients , with a significant decrease of T cell antigen receptor ( TCR ) delta and CD 56 gene expression , associated with a concomitant increase of TCRalpha and CD8beta that were correlated with cytotoxic factors , proinflammatory chemokines , and chemokine receptors including peforin , interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , RANTES , and CXCR 3 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Positive correlations between key regulatory cytokines IL 10 and IFN gamma , IL 10 and IL 12 , and IL 10 and TNF alpha suggests that there may be some level of an intralesional pro or anti inflammatory mechanism essential in avoiding immunopathology . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Culture supernatant of maternal intervillous leukocytes obtained from infected placentas contained significantly higher levels of TNF alpha , IL 10 , monocyte chemotactic protein 1 , macrophage inflammatory protein ( MIP ) 1alpha , MIP 1beta , and IFN gamma inducible protein 10 than did cultures of white blood cells obtained from uninfected placentas . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , joints with OA showed a more complex cytokine pattern , also involving IFN gamma ( P = 0 . 019 ) , IL ra ( P = 0 . 047 ) , and apparently but without reaching the chosen level of significance , IL 2 , TNF alpha and TGF beta 1 , 2 , 3 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Priming of neutrophils by TNF alpha , IFN gamma , or C5adesArg did not promote an oxidative burst or uptake of sIgA opsonized S . aureus to a greater extent than with IgG 1 opsonized bacteria . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This pattern showing a lack of E 2 dependent inhibition of EAE in Esr 1 / mice was mirrored by an enhanced rather than a reduced secretion of TNF alpha , IFN gamma , and interleukin ( IL ) 6 in MOG specific splenocytes and a lack of inhibition of message for inflammatory cytokines , chemokines and chemokine receptors in CNS tissue . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When expression of mRNA for the light ( MRP 8 ) and heavy ( MRP 14 ) calprotectin chain was determined by RT PCR , their respective levels were shown to be increased four to ninefold and three to fivefold after 24 h of combined stimulation with IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Thirty six patients with a variety of symptoms at acute infection and follow up ( mean , 22 . 0 months ) and controls ( 99 330 , depending on the locus ) were examined for the following cytokine polymorphisms : tumour necrosis factor alpha ( TNF alpha ) , 308 ; interferon gamma ( IFN gamma ) , +874 ; interleukin 6 ( IL 6 ) , 174 ; IL 10 , 592 , 819 , and 1082 ; and transforming growth factor beta 1 ( TGF beta 1 ) , +869 ( codon 10 ) and +915 ( codon 25 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The responses of these fibroblasts to TNF alpha , IFN gamma and IL 4 stimulation were markedly different , as revealed by hierarchical cluster analysis and principal component analysis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
None of the factors TNF alpha , TGF beta 1 , or IFN gamma altered ADM secretion in either normal or PE placentas . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using a multiparameter cytokine plating method , we analyzed osteoclastogenesis in response to multiple combinations of the following inflammation related cytokines : RANKL , IFN gamma , TNF alpha , IL 1beta , IL 6 , IL 10 . ^^^ We further investigated the role of T cell related cytokine combinations on osteoclastogenesis by measuring osteoclast area in response to RANKL with IFN gamma , IL 2 , IL 4 , IL 6 , TGF ss , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The main objective of this assay was to prove the relationship between female lactation , which implies hyperprolactinaemia ( IFN gamma , IL 12 , IL 2 and TNF alpha elevated ) , and leishmaniosis ( IFN gamma , IL 12 , IL 2 and TNF alpha diminished ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Administration of PL in vivo decreases IL 2 , IFN gamma and TNF alpha production in splencotyes and enhances spontaneous cell apoptosis in macrophages and lymphocytes stimulated with LPS in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Plasma levels of interleukin ( IL ) 1 beta , IL 2 , IL 4 , IL 8 , IL 10 , IL 12 p 70 , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) were determined by quantitative ELISA during the course of disease respectively . 12 healthy blood donors were used as normal controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We previously reported that interleukin 1beta ( IL 1beta ) alone does not cause apoptosis of beta cells , whereas when combined with gamma interferon ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) , it exerts a distinct apoptotic effect . ^^^ Exposure to IL 1beta for 24 h decreased cellular IB1 / JIP 1 content by 66 + / 17 % ; this IL 1beta effect was maintained in the presence of TNF alpha + IFN gamma , which did not influence IB1 / JIP 1 levels by themselves . ^^^ Addition of IL 1beta to TNF alpha + IFN gamma increased apoptosis from 20 + / 2 % to 59 + / 5 % . ^^^ A similar increase in TNF alpha + IFN gamma induced apoptosis was produced by adenoviral expression of antisense IB1 / JIP 1 and was not further enhanced by addition of IL 1beta , indicating that IL 1beta mediated suppression of IB1 / JIP 1 in beta cells increases their susceptibility to cytokine induced apoptosis . ^^^ However , adenovirally mediated overexpression of IB1 / JIP 1 also potentiated TNF alpha + IFN gamma induced apoptosis , suggesting that the antiapoptotic effect of IB1 / JIP 1 depends on well defined cellular levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Gammadelta T lymphocytes from cystic fibrosis patients and healthy donors are high TNF alpha and IFN gamma producers in response to Pseudomonas aeruginosa . ^^^ METHODS : The aim of the present work was to study intracytoplasmic IL 2 , IL 4 , IFN gamma and TNF alpha production by gammadelta and alphabeta T lymphocytes from cystic fibrosis patients and healthy donors in response to Pseudomonas aeruginosa ( PA ) . ^^^ RESULTS : The highest production of cytokine was of TNF alpha and IFN gamma , gammadelta being better producers than alphabeta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Cultured human umbilical vein endothelial cells ( HUVECs ) were exposed to AGEs BSA for 6 , 12 , 24 , and 36 hours , and exposed to AGEs BSA glycosylated with different concentrations of glucose , tumor necrosis factor alpha ( TNF alpha ) , interferon ( IFN gamma ) , TNF alpha + IFN gamma and AGEs BSA + TNF alpha for 24 hours , respectively . ^^^ However , PECAM 1 expression was reduced in the cells treated with TNF alpha , IFN gamma , TNF alpha + IFN gamma and AGEs BSA + TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To better understand the regulation of adenosine receptors during these processes we studied the effects of IL 1 , TNF alpha , and IFN gamma on expression and function of adenosine receptors and select members of their coupling G proteins in human dermal microvascular endothelial cells ( HMVEC ) . ^^^ The nonselective adenosine receptor agonist 5 ' ( N ethylcarboxamido ) adenosine increased cAMP levels in both TNF alpha and IFN gamma treated cells , but not control cells , and its effect was only partially reversed by ZM 241385 in TNF alpha treated cells and not affected in IFN gamma treated cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Unexpectedly , the administration of anti B 7 H1 , but not anti B 7 DC , mAb after transfer of CD 4 ( + ) CD45RB ( high ) T cells suppressed wasting disease with colitis , abrogated leukocyte infiltration , and reduced the production of IFN gamma , IL 2 , and TNF alpha , but not IL 4 or IL 10 , by lamina propria CD 4 ( + ) T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The inability to clear P . aeruginosa in the lung correlated with reduced phagocytosis of the bacteria by alveolar macrophages ( AMs ) , but not neutrophils , decreased production of TNF alpha by AMs , and decreased levels of TNF alpha and IFN gamma in the bronchoalveolar lavage fluid following infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OBJECTIVES : The purpose of this study was to determine the effects of estradiol and progesterone on interferon gamma ( IFN gamma ) , interleukin ( IL ) 12 , IL 10 and tumor necrosis factor alpha ( TNF alpha ) productions in polyclonal activators ( phytohemagglutinin+lipopolysaccharide ) stimulated whole blood cultures . ^^^ The supernatant , then , was harvested and assayed for IL 12 p 70 , IFN gamma , IL 10 and TNF alpha . ^^^ RESULTS : At preovulatory concentrations , estradiol enhanced significantly IFN gamma , IL 12 and IL 10 , but not TNF alpha , production levels and reversed the suppressive effect of hydrocortisone in PHA+LPS stimulated whole blood . ^^^ No effect of progesterone on IL 12 p 70 , IFN gamma , IL 10 and TNF production levels was observed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines such as IL 2 and IFN gamma enhance T cell expansion , induce cytotoxic T cells ( CTL ) and natural killer ( NK ) cell responses , and prime additional mononuclear phagocytes to produce TNF alpha and IL 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Synergy between IFN gamma and inulin was mainly dependent on inulin induced TNF alpha secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
An important part of the pathogenesis is epithelial damage , stimulating the production of inflammatory cytokines ( mainly IL 1 , TNF alpha , IFN gamma ) , adhesive molecules and acute phase proteins . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We immobilized mice in two consecutive nights , and then determined the cell number , apoptosis , NK cell content , NK activity and the level of cytokine mRNAs ( TNF beta , TNF alpha , IL 4 , IL 5 , IL 1alpha , IFN gamma , IL 2 , IL 6 , IL 1beta and IL 3 ) in the thymus and spleen . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast to LPS , stimulation of PBMC with live Borrelia , increases also the production of IL 12 and IFN gamma , besides IL 1 beta , TNF alpha , IL 6 and IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of cytokines such as IL 1beta , IL 2 , IL 6 , IL 12 and IFN gamma in AMG and L NAME treated grafts were significantly decreased ( P < 0 . 01 ) , whereas IL 10 , TNF alpha and TGF beta 1 were unchanged or enhanced . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 10 , IFN gamma and TNF alpha are key components in the regulation of immune responses and the balance of their expression levels is predictive in certain diseases . ^^^ To describe cytokine genotypes , a one tube PCR reaction was developed to analyse simultaneously DNA sequence variations of cytokine genes IL 10 , IFN gamma , and TNF . ^^^ This assay may improve immunogenetic studies in disease , characterized by disbalanced IL 10 , IFN gamma and TNF alpha expression . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokines ( IFN gamma , TNF alpha and IL 2 ) levels in serum were measured by enzyme linked immunosorbent assay ( ELISA ) . ^^^ The IFN gamma and TNF alpha levels were much higher in mutant strain group than those in wild strain group ( t=2 . 584 , 4 . 766 , P < 0 . 01 ) , so was the ratio of CD4+ / CD8+ ( t=2 . 275 , P < 0 . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , low doses of IFN gamma ( < 5 ng . ml ( 1 ) ) ( < 400 IU . ml ( 1 ) ) increased the resistance to the cytotoxic effect of tumor necrosis factor alpha in the RT 112 cells but not in the RT 4 cells . ^^^ CONCLUSIONS : We demonstrated that in the low grade bladder cancer cell lines , the effect of IFN gamma was dose dependent : high doses ( > 5 ng . ml ( 1 ) ) induced apoptosis of RT 4 and RT 112 cells , whereas low doses ( < 5 ng . ml ( 1 ) ) induced a resistance to the cytotoxic effect of tumor necrosis factor alpha and increase the metastatic potential of the RT 112 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Islets of Langerhans isolated from diabetes prone BB / OK rats were exposed to interleukin 1beta ( IL 1beta ) or to a combination of tumor necrosis factor alpha ( TNF alpha ) plus interferon gamma ( IFN gamma ) under hypoglycemia at glucose concentrations of 2 . 2 and 3 . 2 mmol / l or in the presence of stimulatory conditions at 6 . 0 and 11 mmol / l glucose . ^^^ Islets pre exposed for 24h to TNF alpha plus IFN gamma at 2 . 2 , 3 . 2 or 6 . 0 mmol / l glucose displayed no alterations of insulin secretion following a 48 h regeneration . ^^^ In contrast , a 3 h treatment with TNF alpha plus IFN gamma at 11 mmol / l glucose did not affect insulin secretion of islets prior held at 6 . 0 mmol / l glucose , whereas a transient exposure for 6h to IL 1beta as well as TNF alpha plus IFN gamma under similar conditions diminished insulin secretion of islets preincubated at 2 . 2 or 6 . 0 mmol / l glucose . ^^^ TNF alpha plus IFN gamma at concentrations capable to decrease insulin secretion of islets during hyperglycemia do not affect the insulin output in a range between 2 . 2 and 6 . 0 mmol / l glucose . ^^^ During glucose stimulation at 11 mmol / l islets ' insulin secretory machinery is protected from IL 1beta as well as TNF alpha plus IFN gamma for 3 h by a preceding 24 h hypoglycemia , but its vulnerability is restored within additional 3 h . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , when neutrophils were exposed to Fas ligand , TNF alpha , or TRAIL , the anti apoptotic effects of granulocyte macrophage colony stimulating factor ( GM CSF ) , granulocyte colony stimulating factor ( G CSF ) , or IFN gamma were blocked , most likely by SHP 1 mediated inactivation of anti apoptotic signaling molecules . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The frequencies of HCMV specific CD4+ T cells in pregnant women with serum anti HCMV IgG antibody were detected by intracellular cytokine ( ICC ) , interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) staining . ^^^ RESULTS : The levels of intracellular cytokines in pregnant women with serum anti HCMV IgG antibody were significantly higher than those in women without anti HCMV IgG antibody ( P = 0 . 011 for IFN gamma and P = 0 . 023 for TNF alpha ) but lower than those in non pregnant women with serum anti HCMV IgG antibody . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Isolated crypts and cultured cancer cells were stimulated with interferon gamma ( IFN gamma ) or tumor necrosis factor alpha ( TNF alpha ) . ^^^ In contrast , HT 29 cells and the collagen embedded crypts expressed ICAM 1 on the apical membranes proximal to the junctional complexes when stimulated with IFN gamma or TNF alpha in a dose related manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects observed on ECs cocultured with in vivo activated CD 28 ( ) cells were partly reproduced when ECs were grown in medium containing interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^ We , therefore , conclude that CD 28 ( ) T lymphocytes from HIV 1 infected patients exert their effect on ECs through a mechanism involving both IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The peripheral blood mononuclear cells ( PBMCs ) isolated from those subjects were stimulated with HBcAg 18 to 27 CTL epitope peptide , and intracellular cytokine staining ( ICCS ) was used for detecting IFN gamma , IL 2 and TNF alpha produced by CD8+ T cell . ( 2 ) DCs generated from PBMCs were pulsed with HBcAg 18 to 27 CTL epitope peptide , then were cocultured with autologous lymphocytes for 10 days to induce antigen specific T cell , which was assessed by ICCS and cytotoxic assay . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Surviving rats were euthanized on day 150 and intragraft gene ( CD 80 , 86 , 152 , 154 , IFN gamma , IL 2 , IL 6 , IL 10 , IL 13 , TNF alpha , TGF beta , IL 7 , Fas ligand , Granzyme B , bax , and bcl ( 2 ) ) and protein ( CD 4 , CD 8 , ED 1 , CD 154 , CD 80 , CD 86 ) expression was measured . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We determined the concentrations of Th 1 mediated ( IFN gamma , TNF alpha ) and Th 2 mediated ( IL 4 ) cytokines in the sera of patients with tungiasis . ^^^ The results show that tungiasis causes a mixed Th 1 and Th 2 immune response , characterized by significantly increased concentrations of the pro inflammatory cytokines IFN gamma and TNF alpha , with a slightly increased concentration of IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Therefore our study aimed at examining the mRNA expression pattern of tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , interleukin 6 ( IL 6 ) and interleukin 10 ( IL 10 ) in whole blood of 11 female AN patients and 10 age and sex matched normal weight control subjects using a sensitive quantitative polymerase chain reaction ( PCR ) method . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When cells were treated with interleukins ( IL 1beta , IL 6 ) , tumor necrosis factor alpha ( TNF alpha ) , or interferon gamma ( IFN gamma ) , or combinations of these , real time PCR demonstrated that MUC 1 mRNA increased 1 . 4 to 3 . 2 fold . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Either anti IFN gamma or TNF alpha antibody inhibited the enhancement of liver injury . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We show that E . coli LPS pulsed MDDC released Th 1 biasing cytokines consisting of high levels of IL 12 p 70 , IFN gamma inducible protein 10 ( IP 10 ) but also TNF alpha , IL 10 , IL 6 and IL 1beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Allergina alone or Allergina plus recombinant IFN gamma ( rIFN gamma ) increased the production of tumor necrosis factor ( TNF ) alpha , but Allergina decreased the production of TNF alpha on rIFN gamma plus LPS stimulated macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 12Rbeta1 ( / ) CD 4 ( + ) T cells , in turn , appear to exert an immunoregulatory effect on the capacity of wild type APCs to produce IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Two weeks later , the serum levels of IL 18 , interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) and nitric oxide ( NO ) in the implanted liver carcinoma bearing mice were assayed , the cytotoxicity of murine splenic cytotoxic T lymphocytes ( CTLs ) was measured , and the morphology of the hepatic tumors was studied to evaluate the antitumor effects of the approach . ^^^ RESULTS : In the treatment group , the serum levels of IL 18 , IFN gamma , TNF alpha and NO increased significantly . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
ELISPOT assays demonstrated a high frequency of cells secreting proinflammatory tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and interleukin 4 ( IL 4 ) in the lungs in response to infection with KyA or RacL 11 or immunization with rgD . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukins IL 1alpha , IL 2 , IL 4 , IL 6 , IL 8 , IL 10 , tumor necrosis factor ( TNF alpha ) and interferon gamma ( IFN gamma ) as well as IL 2 receptor ( IL 2R ) were quantified at mRNA level using RT PCR . ^^^ In the vaccination group , TNF alpha and IL 6 tended to be increased at PIDs 22 and 40 , whereas no increase could be seen in IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OBJECTIVE : The aim of this study was to investigate whether the serum levels of soluble interleukin 2R ( sIL 2R ) , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , IL 6 , IL 10 , and transforming growth factor beta ( 1 ) ( TGF beta ( 1 ) ) were associated with the development of acute graft vs host disease ( aGVHD ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum levels of IFN gamma , TNF alpha , IL 18 and IL 6 increased in the postoperative phases as compared to the preoperative period . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CXCR 4 surface expression on neutrophils but not on lymphocytes and monocytes is rapidly down regulated after stimulation with TNF alpha and IFN gamma , resulting in significantly decreased SDF induced functional responses of neutrophils . ^^^ By confocal microscopy we demonstrate that CXCR 4 is internalized after stimulation with TNF alpha and IFN gamma . ^^^ Tauhe down modulation of CXCR 4 surface expression in response to TNF alpha and IFN gamma was fully reversible after cytokine removal . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human TNF alpha , but not human IFN gamma , induced class 1 , but not class 2 , MHC expression on PBMEC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Production of intracellular IL 2 , TNF alpha , and IFN gamma by T cells in B CLL . ^^^ We studied the intracellular expressions of interleukin 2 ( IL 2 ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) in T helper 1 cells ( Th 1 response ) of B CLL . ^^^ No significant differences in TNF alpha or IFN gamma intracellular expressions were found between patients and healthy individuals . ^^^ TNF alpha and IFN gamma expressing CD 8 T cells were disease stage dependent , being significantly higher in late stage patients ( P < 0 . 001 for both cytokines ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : In eight patients with localized intra abdominal infection ( diverticulitis ) and six patients with peritonitis due to perforation of a hollow viscus , we examined the cytokine response of CD 3 ( + ) T cells in the greater omentum and in the peripheral blood by in vitro stimulation , intracellular cytokine staining , and flow cytometry for TNF alpha , IFN gamma , IL 2 , and IL 4 . ^^^ RESULTS : In localized infections ( diverticulitis ) , there was higher expression of TNF alpha ( 51 % / 35 % positive cells in omentum / blood ) , IFN gamma ( 47 % / 32 % ) , and IL 2 ( 33 % / 20 % ) in the omentum than in the peripheral blood mononuclear cells ( PMBCs ) , but this was not true for IL 4 expression ( 0 . 8 % / 1 . 3 % ) . ^^^ In patients with peritonitis , there were no differences in cytokine expression between lymphocytes from the greater omentum and from PMBCs for TNF alpha ( 18 % / 21 % omentum / blood ) , IFN gamma ( 20 % / 22 % ) , IL 2 ( 16 % / 12 % ) , or IL 4 ( 10 . 9 % / 7 . 6 % ) . ^^^ Compared to the diverticulitis group , patients with peritonitis showed reduced expression for TNF alpha , IFN gamma , and IL 2 , but there was a significantly higher response for IL 4 for both compartments . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In eukaryotes , RNA sensors in human antiviral cytokine genes that encode tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) have been shown to activate strongly the RNA dependent protein kinase PKR , a stress kinase that is also activated by double stranded RNA a hallmark of viral infection . ^^^ These cis acting RNA elements in the TNF alpha and IFN gamma transcripts function as sensors of intracellular PKR levels and regulate gene expression at the level of mRNA splicing and translation , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The numbers of cells spontaneously secreting IFN gamma , IL 12 , and TNF alpha were significantly increased in patients with active BD . ^^^ A favorable clinical response to infliximab was associated with a persistent reduction in TNF alpha secretion , but did not correlate with IFN gamma production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Spleen cells from mice undergoing withdrawal also had decreased splenic mRNA and / or protein levels of IL 1beta , IL 1Ra , TNF alpha , IL 12 , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin 1beta ( IL 1beta ) , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) and transforming growth factor beta 1 ( TGF beta 1 ) were measured using enzyme linked immunosorbant assay ( ELISA ) kits . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Regulation of TNF alpha and IFN gamma induced CXCL 10 expression : participation of the airway smooth muscle in the pulmonary inflammatory response in chronic obstructive pulmonary disease . ^^^ In primary cultured hASM cells taken from normal donors , CXCL 10 protein expression was induced by IFN gamma and TNF alpha , cytokines reported as elevated in COPD , and a synergistic response was obtained when they were combined . ^^^ TNF alpha stimulation of hASM enhanced accumulation of CXCL 10 mRNA , indicating regulation at the transcriptional level , while IFN gamma stimulation resulted in a smaller accumulation of CXCL 10 mRNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These cells secreted large amounts of TGF beta and TNF alpha with little or no IFN gamma and IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here , some of the promising cytokines currently under investigation for cancer therapies are examined , including interleukin ( IL ) 2 , IL 4 , IL 12 , IL 24 , interferon ( IFN ) alpha , IFN beta , IFN gamma , granulocyte monocyte colony stimulating factor and tumor necrosis factor ( TNF ) alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The other horn was flushed with 20 mL of PBS , and the contents of interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) and transforming growth factor beta 1 ( TGF beta 1 ) were determined by ELISA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated whether induction of two cytotoxic compounds , tumor necrosis factor alpha ( TNF alpha ) and nitric oxide ( NO ) , directly caused IAV induced apoptosis , and whether induction could be modulated by interferon gamma ( IFN gamma ) or the replication competence of the virus . ^^^ When cells were pre treated with IFN gamma , inactive IAV became as effective an inducer of NO , but not TNF alpha , as live IAV . ^^^ Amantadine , which antagonizes viral entry and replication , partly inhibited TNF alpha and NO production in unprimed cells , but did not inhibit NO in IFN gamma primed cells . ^^^ Importantly , viral replication was not required for the induction of TNF alpha or NO , and the action of inactive IAV could be potentiated by IFN gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
ICOSL expression is upregulated by the inflammatory cytokine , tumor necrosis factor alpha ( TNF alpha ) , whereas interferon gamma ( IFN gamma ) has no such effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Preincubation of control CLPF with TNF ( 20 ng / mL ) and IFN gamma ( 10 ng / mL ) for 3 days reduced their migratory response to 10 % of control ( P < 0 . 001 ) , which also was correlated with a decrease in FAK phosphorylation . ^^^ Culture of IFN gamma / TNF treated CLPF for a further 7 days without cytokines did not restore the migratory potential and FAK phosphorylation , indicating a persistent functional change . ^^^ That may be caused by contact with IFN gamma and TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NPY , NPY receptors , and DPP 4 activity are modulated by LPS , TNF alpha and IFN gamma in HUVEC . ^^^ Since NPY increases endothelial cell ( EC ) stickiness for leukocytes , we studied the effects of LPS , TNF alpha and IFN gamma on its expression and action in HUVEC . ^^^ Y 1 and Y 2 receptors were expressed in basal conditions , and LPS , TNF alpha and IFN gamma induced Y 5 receptor expression with a concomitant extinction of Y 2 receptor expression . ^^^ After stimulation with LPS , TNF alpha and IFN gamma , calcium increase was mainly caused by Y 5 receptor . ^^^ The modulation of the NPY system by LPS , TNF alpha and IFN gamma , and the NPY induced calcium signaling suggest a role for NPY during the inflammatory response . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
SAgs bind as intact proteins to the DRalpha 1 domain of the MHC class 2 receptor and the TcRVbeta domain from the T cell receptor and cause excessive release of cytokines such as IL 2 , TNF alpha , and IFN gamma , and hyperproliferation of T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : MKN 45 ( wild type p 53 ) or MKN 28 ( mutant type p 53 ) was cultured with TNF Alpha or IFN Gamma either alone or together . ^^^ To clarify whether the mechanism of apoptosis is induced by TNF Alpha and / or IFN Gamma , apoptosis related genes were examined by a cDNA on microarray analysis and a Western blot analysis . ^^^ RESULTS : ( 1 ) The AI for MKN 45 increased at 48 h in the presence of TNF Alpha or IFN Gamma alone . ( 2 ) In the case of combination treatment using TNF Alpha and IFN Gamma , the AI for MKN 45 was higher than those in the groups with a single treatment . ( 3 ) A cDNA microarray analysis showed that IGF BP 3 , the TNF superfamily , and caspase 1 were all upregulated after treatment with the combination of TNF Alpha and IFN Gamma . ( 4 ) A Western blot analysis of MKN 45 showed a reaction with an anti IGF BP 3 antibody . ^^^ CONCLUSIONS : These results suggest that the induction mechanism underlying apoptosis induced by TNF Alpha and IFN Gamma might therefore involve the caspase independent pathway via IGF BP3 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To examine the host response to acute infection , we collected paired serum specimens from 30 children with rotavirus diarrhea and measured the levels of nine cytokines ( interleukin 1beta [ IL 1beta ] , IL 2 , IL 4 , IL 6 , IL 8 , IL 10 , IL 12 , gamma interferon [ IFN gamma ] , and tumor necrosis factor alpha [ TNF alpha ] ) using a microsphere based Luminex Flowmetrix system . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Various adhesion molecules and chemokines play key roles in these cell to cell interactions , and the expression of these adhesion molecules and the production of chemokines are regulated by inflammatory cytokines such as tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 ( IL 1 ) , and interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha and IFN gamma cause further injury to gastrointestinal epithelium , causing more endotoxin leakage and establishing a positive inflammatory feedback loop . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha and IFN gamma production from memory CD4+ T cells were measured and compared to a control group who never received the anthrax vaccine . ^^^ The optimal antigen concentration for TNF alpha was determined to be around 7 . 5 microg / ml and IFN gamma production was not detected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
STAT 1 alpha and IFN gamma as modulators of TNF alpha signaling in macrophages : regulation and functional implications of the TNF receptor 1 : STAT 1 alpha complex . ^^^ TNF alpha and IFN gamma cooperate in the activation of macrophages . ^^^ TNF alpha dependent activation of NF kappaB is stronger in the presence of IFN gamma . ^^^ We hypothesized that nuclear localization of STAT 1alpha due to IFN gamma signaling would preclude it from being recruited to the TNFR 1 and therefore enhance TNF alpha induced NF kappaB activation . ^^^ In the RAW264 . 7 macrophage cell line , TNF alpha treatment indeed recruits STAT 1alpha to the TNFR 1 , and this association is abrogated when cells are exposed to IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Administration of mAb to TNF alpha or IFN gamma , but not GM CSF , abrogated protection in naive IL 10 ( / ) mice ; mAb to TNF alpha , but not IFN gamma or GM CSF , subverted protective immunity in secondary histoplasmosis . ^^^ The inflammatory cell composition in IL 10 ( / ) mice was altered in those given mAb to IFN gamma or TNF alpha . ^^^ More Gr 1 ( + ) and Mac 3 ( + ) cells were present in lungs of IL 10 ( / ) mice given mAb to IFN gamma , and treatment with mAb to TNF alpha sharply reduced the number of CD 8 ( + ) cells in lungs of IL 10 ( / ) mice . ^^^ Hence , deficiency of IL 10 confers a salutary effect on the course of histoplasmosis , and the beneficial effects of IL 10 deficiency require endogenous TNF alpha and / or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , infection with the lethal strain Py17XL induces high levels of circulating TGF beta within 24 h ; this is associated with delayed and blunted IFN gamma and TNF alpha responses , failure to clear parasites , and 100 % mortality . ^^^ Neutralization of early TGF beta in Py17XL infection leads to a compensatory increase in IL 10 production , while simultaneous neutralization of TGF beta and IL 10R signaling leads to up regulation of TNF alpha and IFN gamma , prolonged survival in all , and ultimate resolution of infection in 40 % of Py17XL infected animals . ^^^ In mice immunized against Py17XL , the early TGF beta response is inhibited and is accompanied by significant up regulation of IFN gamma and TNF alpha and rapid resolution of challenge infections . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pulmonary fibrosis in IL 5 ( TG ) mice was preferentially associated with type 2 cytokines ( IL 4 and IL 13 ) , whereas fibrotic lesions in IL 5 ( / ) animals were accompanied by proinflammatory cytokine ( TNF alpha , IL 1beta , and IFN gamma ) expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Effector CD 4 cell tolerization is mediated through functional inactivation and involves preferential impairment of TNF alpha and IFN gamma expression potentials . ^^^ In the present study , we found that within 24h self antigen profoundly impairs the ability of CD 4 effectors to express TNF alpha ( and to a lesser extent IFN gamma ) ; however , several days of self antigen exposure is required to impair non effector functions such as IL 2 expression and proliferation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Caco 2 / TC7 and HCT 8 cells were stimulated with a cytokine mixture ( IL 1 beta , TNF alpha , IFN gamma ) and duodenal biopsies from human healthy volunteers in organ culture were stimulated with IL 1 beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The changes in mRNA expression of inflammation related cytokines including mouse analogue of human monocyte chemoattractant protein 1 ( MCP 1 ) ( JE ) , mouse analogue of human interleukin 8 ( IL 8 ) ( KC ) , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , IL 12p40 , IL 6 , IL 10 , Eotaxin were determined in mouse epidermal cells treated by 2 % retinol using a semi quantitative reverse transcription polymerase chain reaction ( RT PCR ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pro inflammatory cytokine levels of Interleukin 2 ( IL 2 ) , Interleukin 6 ( IL 6 ) , Interferon gama ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) were depressed in HIV infected volunteers compared to healthy volunteers and non HIV infected burn patients . ^^^ The pro inflammatory cytokine IFN gamma did not increase after burn injury in HIV infected burns patients as did IL 2 , IL 6 and TNF alpha ( p < 0 . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Portions of the cell samples were cultured for 4 h in the presence of 1 microm monensin and 20 microg / ml brefeldin A with or without stimulation by phorbol myristate acetate plus ionomycin for the detection of intracellular interferon gamma ( IFN gamma ) , interleukin 2 ( IL 2 ) , tumour necrosis factor ( TNF ) alpha , and IL 4 . ^^^ As a result , CD4+CD29high helper inducer T cells were closely associated with IFN gamma and TNF alpha producing CD4+ cells , while CD4+CXCR3+ cells showed a negative correlation with IL 4 producing cells , suggesting that both of these CD4+ subsets consist mainly of Th 1 cells . ^^^ In contrast , CD4+CD45RA+ cells were correlated inversely with IFN gamma and TNF alpha producing cells , and CD8+CD11ahigh killer effector and total CCR5+ cells showed an inverse correlation with IL 2 producing cells , suggesting an immunoregulatory role for these three subsets in non pathological conditions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Ebastine inhibited T cell proliferation and the production of IL 4 , IL 5 , IL 6 , and TNF alpha by T cells under each co stimulatory condition tested , whereas it exhibited no effect on the production of IL 2 or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
One of these cytokines , IL 15 , triggers inflammatory cell recruitment , angiogenesis , and production of other inflammatory cytokines , including IFN gamma , TNF alpha , and IL 17 , which are all upregulated in psoriatic lesions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Analysis of the regulation demonstrated that the expression of CCL 2 was significantly elevated and in a synergistic manner by IFN gamma , tumor necrosis factor alpha , and interleukin 1beta . ^^^ Moreover , other proinflammatory cytokines such as tumor necrosis factor alpha and IFN gamma appeared able to induce apoptosis and to reduce the proliferative rate of pancreatic cancer . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : PCR using sequence specific primer ( PCR SSP ) was performed to determine the cytokine genotypes of tumor necrosis factor alpha ( TNF alpha ) , interleukin 10 ( IL 10 ) , transforming growth factor beta 1 ( TGF beta 1 ) , interleukin 6 ( IL 6 ) , interferon gamma ( IFN gamma ) in 97 sensitized kidney transplant recipients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma rapidly primes the macrophage via JAK1 / 2 STAT 1 pathway so that it can subsequently undergo a slower classical type 1 activation upon exposure to T helper ( Th ) 1 cytokines such as IFNgamma or other activators , including tumor necrosis factor and lipopolysaccharide , e . g . in intracellular killing of phagocytosed Mycobacterium tuberculosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Active celiac disease was characterized by distortions in cytokine expression by T lymphocytes , with highly significant increases of IFN gamma and IL 10 but no concomitant increases in tumor necrosis factor alpha , transforming growth factor beta 1 , or IL 2 and no induction of IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inflammation is progressed by the action of pro inflammatory cytokines , including IL 1 , TNF , IFN gamma and IL 6 , and is resolved by anti inflammatory cytokines , such as IL 4 , IL 10 , IL 13 , IFN alpha and transforming growth factor beta ( TGF beta ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RT PCR analysis of the corneas of rIL 12 vs PBS / BSA treated mice showed a significant increase in IFN gamma and TNF alpha mRNA levels in the rIL 12 vs PBS / BSA ( vehicle ) treated mice at 3 and 5 days p . i . ^^^ CONCLUSIONS : These data provide evidence that BALB / c mice can respond to exogenous IL 12 , that the cytokine promotes susceptibility by increasing IFN gamma and TNF alpha production , with a concomitant reduction in IL 4 levels ; and that injected rIL 4 fails to rescue susceptible B 6 mice from corneal perforation after bacterial challenge . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The number of clinical studies on gamma interferon ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) and interleukin 4 ( IL 4 ) in human brucellosis is limited . ^^^ The present study was focused on IFN gamma , TNF alpha and IL 4 levels in acute brucellosis cases , in the acute phase and at the end of the treatment . ^^^ It was found that mean IFN gamma and TNF alpha levels , CRP ( C reactive protein ) levels and ESR ( erythrocyte sedimentation rate ) values were significantly higher in acute brucellosis cases as compared to post treatment values and values measured in the control group . ^^^ In addition , IFN gamma and TNF alpha levels measured in the acute phase correlated with the increase in CRP levels and ESR values . ^^^ Our results confirmed that IFN gamma and TNF alpha are involved in the pathophysiology of brucellosis and are closely related to the inflammatory activation of the disease . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
H . pylori alone or in combination with Th 1 cytokines ( IFN gamma and TNF alpha ) significantly increased the expression of Fas in gastric epithelial cell lines in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Main results were : NK cell lytic activities against both the NK susceptible K 562 cell line and the NK resistant Daudi cell line were significantly augmented in EU IDUs compared with either controls or seroconverters before or after seroconversion ; NK cells producing the cytokines IFN gamma and TNF alpha and the beta chemokines CCL 3 , CCL 4 , and CCL 5 were also increased in the EU IDUs , either after in vitro activation or without stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Titres of endogenous IFN gamma and tumour necrosis factor alpha ( TNF alpha ) were markedly increased in the spleens and livers of IL 6 / mice in comparison with IL 6+ / + mice at days 1 through 3 p . i . ^^^ In vivo administration of either an anti IFN gamma monoclonal antibody ( mAb ) or anti TNF alpha mAb to IL 6 / mice reduced the number and size of granulomas , and prevented formation of necrotic granulomas . ^^^ In addition , the production of endogenous IFN gamma and TNF alpha in the early phase of R . aurantiacus infection by IL 6 / mice was suppressed by treatment with recombinant IL 6 ( rIL 6 ) . ^^^ This suppression of IFN gamma and TNF alpha production was followed by a reduction in the number and size of central necrotic granulomas at 2 weeks p . i . ^^^ These findings suggest that overproduction of IFN gamma and TNF alpha induces central necrotic granuloma formation in IL 6 / mice , and that IL 6 down regulates granulomatous inflammation reaction in response to R . aurantiacus infection by modulating production of IFN gamma and TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In combination with TNF alpha , IFN gamma suppressed CXCL 8 but enhanced CCL 5 and CXCL 10 , whereas transforming growth factor ( TGF ) beta reduced secretion of all chemokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : We activated CD 40 in HPMC along with combinations of TNF alpha , interleukin 1 ( IL 1 ) , and interferon gamma ( IFN gamma ) , and evaluated the mRNA levels and protein secretion of regulated upon activation , normal T cell expressed and secreted ( RANTES ) , monocyte chemoattractant protein 1 ( MCP 1 ) , and IL 8 . ^^^ RESULTS : CD 40 ligation had a small stimulatory effect on the secretion of all three chemokines , while TNF alpha , IL 1 and IFN gamma induced their secretion in a dose dependent manner . ^^^ In contrast , the secretion of the neutrophil chemoattractant IL 8 , induced by CD 40 ligation or by the combination of IL 1 and TNF alpha , was reduced in the presence of IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Importantly , effects of retinoids on the MHC class 1 pathway appear to be independent of IFN gamma and / or TNF alpha as intermediate messengers . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inflammation is characterized by an influx into the mucosa of immune cells that influence epithelial function by releasing proinflammatory cytokines such as IFN gamma and TNF alpha . ^^^ Since the AJC regulates barrier function , we analyzed the influence of IFN gamma and TNF alpha on its structure / function and determined the contribution of apoptosis to this process using a model intestinal epithelial cell line , T 84 , and IFN gamma and TNF alpha . ^^^ IFN gamma induced time dependent disruptions in epithelial gate function that were potentiated by coincubation with TNF alpha . ^^^ Our findings for the first time clearly separate the proapoptotic effects of IFN gamma and TNF alpha from their abilities to disrupt barrier function . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Fas , Fas ligand , FLIP , TNF alpha , inducible NO synthase , TGF beta , and IFN gamma were highly expressed by infiltrating cells when G EAT progressed to fibrosis . ^^^ There was also relatively less expression of TGF beta , IFN gamma , TNF alpha , and inducible NO synthase and higher expression of IL 10 in resolving G EAT than in G EAT that progressed to fibrosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine profiles of stimulated PBMCs were determined by intracellular cytokine staining ( IL 5 , IL 10 , IL 12 p 40 , TNF alpha , and IFN gamma ) and cytometric bead array ( IL 2 , IL 4 , IL 5 , IL 10 , TNF alpha , and IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Th 1 cells produce cytokines : interleukin 2 , IFN gamma , TNF alpha , which are responsible for effective activation of CTL cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Gene knockout and wildtype mice were infected intranasally with the avirulent A 7 strain of SFV and neuropathological lesions were correlated with levels of IFN gamma , tumour necrosis factor ( TNF ) alpha and interleukin ( IL ) 10 in the olfactory bulbs and frontal cortex . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The time kinetics of five cytokines [ interleukin 2 ( IL 2 ) , IL 5 , interferon gamma ( IFN gamma ) , granulocyte macrophage colony stimulating factor ( GM CSF ) and tumour necrosis factor alpha ( TNF alpha ) ] and one cytotoxic effector protein ( granzyme B ) was analysed by real time quantitative polymerase chain reaction ( PCR ) following in vitro stimulation of human CD 4 and CD 8 T lymphocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Macrophage production of TNF and NO was only marginally affected as was splenocyte production of IL 4 and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although no differences in IFN gamma or TNF alpha mRNA were measured , TNF alpha protein expression was greater in affected tissues and demonstrated a shift in the source of TNF alpha from macrophages to lymphocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Nevertheless , bacterial counts in infected lungs , immunohistochemistry , and reverse transcription PCR analysis of mRNAs for tumor necrosis factor alpha ( TNF alpha ) , gamma interferon ( IFN gamma ) , interleukin 1beta ( IL 1beta ) , IL 6 , IL 12 , and inducible nitric oxide synthase ( iNOS ) revealed significant differences among the strains . ^^^ At 72 h postinfection , M90T guaBA purHD still induced proinflammatory cytokines and factors such as IL 1beta , IL 6 , TNF alpha , and iNOS , along with cytokines such as IL 12 and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The mRNA expression patterns of IFN gamma and tumor necrosis factor alpha were examined in parallel , and the results revealed that cytokine mRNA ( + ) cells were abundant and generally localized to the granulomas . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma , TNF alpha , IL 1 beta , and IL 12 also were high in the sera of MyD 88 KO mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The serum level of cytokines including TNF alpha , IFN gamma , IL 6 , and M CSF was elevated , and hemophagocytes were seen in bone marrow . ^^^ The bone marrow CD4+ / CD8+ lymphocyte ratio recovered to within the normal range , and the serum cytokines including TNF alpha and IFN gamma decreased . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RNAse protection assay revealed that DON at 100 to 500 ng / ml induced mRNA expression of TNF alpha as well as IL 6 , IFN gamma , TGFbeta 1 , and TGFbeta 3 and that these effects were potentiated by 100 ng / ml lipopolysaccharide ( LPS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A subset of the cells in cultures of keratinocytes and transformed keratinocyte cell lines died by apoptosis in response to anti Fas , IL 1alpha and TNF alpha plus IFN gamma and ionophore . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , during the internalization process , only coculturing with irradiated CD40L tumor cells resulted in concurrent , optimal DC maturation and production of proinflammatory chemokines and pro Th 1 cytokines , such as IL 6 , IL 8 , IL 12 , IFN gamma , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
DR 4 DRw11 DQw 2 haplotype was more common in preeclamptic women with intrauterine growth restriction , low birth weight and placental weight , increased expression of T helper cytokines IL 8 , TNF alpha and IFN gamma and abnormal uteroplacental vasculature . ^^^ These findings suggest that HLA class 2 DR 4 DRw11 DQw 2 haplotypes may be associated with preeclampsia with intrauterine growth restriction through low placental weight from impaired placental development , as a result of increased expression of T helper 1 cytokines IL 8 , TNF alpha and IFN gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In astrocytes , the tumor necrosis factor ( TNF ) related apoptosis inducing ligand ( TRAIL ) expression level was increased by IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
They were prospectively evaluated for circulating TNF alpha , interferon gamma ( IFN gamma ) , and IL 6 levels at different times , just before and 1 , 2 , 7 , and 21 days after diphosphonate infusion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate the patterns of tumor necrosis factor alpha ( TNF alpha ) , interleukin 1beta ( IL 1beta ) , IL 6 , interferon gamma ( IFN gamma ) and interleukin 1 receptor antagonist ( IL 1Ra ) during the course of dengue shock syndrome . ^^^ MEASUREMENTS : The plasma concentration and the ex vivo production , with and without lipopolysaccharide ( LPS ) , of TNF alpha , IL 1beta and IL 1Ra were measured in duplicate by nonequilibrium radioimmunoassay ( RIA ) ; IFN gamma and IL 6 were measured by ELISA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although the expression of IL 6 and TNF alpha was unresponsive to IFN gamma , the expression of IL 15 was markedly upregulated ( P < 0 . 01 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IGFBP 4 proteolytic activity was increased 4 fold in medium from TNFalpha and IL 1 beta treated ( 1 nm ) cells and decreased 40 % in medium from IFN gamma treated ( 1 nm ) cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We examined cockroach ( Bla g 1 ) induced , house dust mite ( Der f 1 ) induced , and cat ( Fel d 1 ) induced cytokine secretion , including secretion of IFN gamma , IL 13 , IL 10 , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The frequency of T cells expressing proinflammatory Th 1 cytokines ( IFN gamma , TNF alpha , GM CSF , IL 1alpha , and IL 8 ) but not Th 2 cytokines ( IL 4 , IL 5 , IL 6 , IL 10 , and IL 13 ) was significantly increased in response to L 3 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CD 154 activates macrophage antimicrobial activity in the absence of IFN gamma through a TNF alpha dependent mechanism . ^^^ Protection against certain intracellular pathogens can take place in the absence of IFN gamma through mechanisms dependent on TNF alpha . ^^^ These results demonstrate that CD 154 triggers TNF alpha dependent antimicrobial activity in macrophages and suggest that CD 154 regulates the mechanisms of host protection that take place when IFN gamma signaling is deficient . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Exposure of endothelium to proinflammatory cytokines ( IFN gamma , TNF alpha and IL 1beta ) interrupts the BBB by disorganizing cell cell junctions , decreases the brain solute barrier , enhances leukocyte endothelial adhesion and migration as well as increases expression of class 2 MHC and promotes shedding of endothelial ' microparticles ' ( EMP ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Aberrant CD 40 expression by macrophages / microglia , induced by cytokines such as IFN gamma and TNF alpha , contributes to neuroimmunologic cascades in the CNS . ^^^ The signal transduction pathways and transcription factors employed by IFN gamma and TNF alpha to induce CD 40 expression are described , as are the cis elements in the CD 40 promoter that are critical for CD 40 transcription . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
HL 60R exhibits resistance to apoptosis induced from P gp substrate drugs and also from other triggers ( cisplatin , TNF alpha , Fas ligation , TRAIL , IFN gamma and serum starvation ) not related to the multidrug transporter . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) , soluble VEGF receptor 1 ( sVEGFR 1 ) , sVEGFR 2 , transforming growth factor beta 1 ( TGF beta 1 ) , interleukin ( IL ) 6 , IL 8 , IL 12+p40 , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) levels in the supernatants of the aspirates and the culture supernatants of MNCs were then measured by ELISA . ^^^ IL 6 , IL 8 , IL 12+p40 , TNF alpha , and IFN gamma levels were elevated in the culture supernatants of the MNC cultured with OK 432 for up to 9 days . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 12 enhances protective immunity in mice engendered by immunization with recombinant 14 kDa Schistosoma mansoni fatty acid binding protein through an IFN gamma and TNF alpha dependent pathway . ^^^ In an attempt to determine the role of IFN gamma and TNF alpha in IL 12 induced immunity , IFN gamma and TNFR p 55 knockout mice were immunized with rSm14 / IL 12 and no protection was achieved . ^^^ Therefore , protection induced by rSm14 / IL 12 was shown to be dependent on endogenous IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These components include T cells ( both CD4+ and CD8+ ) , cytokines , including IFN gamma , IL 12 , TNF alpha , and IL 6 , and macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The purpose of this work was to obtain bronchoalveolar lavage ( BAL ) fluid from these patients to study the cytopathology , TNF levels and the oxidative and fungicidal response of alveolar macrophages ( AMs ) to in vitro incubation with recombinant IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In a second experiment , alveolar macrophages were incubated with fresh medium ( negative control ) , APP alone , or APP with 1 of the following : IL 1beta , TNF alpha , or IFN gamma . ^^^ PROCEDURE : Alveolar macrophages were incubated for 48 hours with APP ( 1 10 10 ( 4 ) colony forming units / mL ) , interleukin 1beta , ( IL 1beta ; 5 U / mL ) , tumor necrosis factor alpha ( TNFalpha ; 500 U / mL ) , interferon gamma ( IFN gamma , 100 U / mL ) , or lipopolysaccharide ( LPS ; 10 microg / mL ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
All pro inflammatory cytokines decreased in the 24 hour TGF beta 1 active group compared with CAT , TGF beta 1 latent , 18 hour and 1 hour cold ischemia , and non treated lung groups ( IL 1beta , p < 0 . 03 ; TNF alpha , p < 0 . 02 ; IFN gamma , p < 0 . 001 ; IL 2 , p < 0 . 0001 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) play a critical role in the generation of the immune response , leading to the enhanced proinflammatory cytokines and free radicals production . ^^^ TNF alpha and IFN gamma concentrations were measured in the PF using commercially available ELISA kits . ^^^ The positive correlation ( R = 0 . 83 ; p < 0 . 01 ) has been found between TNF alpha and IFN gamma levels . ^^^ Positive correlation between TNF alpha and IFN gamma suggests their synergistic stimulatory effect on the immunocompetent PF cells . . ^^^ Tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) play a critical role in the generation of the immune response , leading to the enhanced proinflammatory cytokines and free radicals production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
ST 1214 markedly inhibited the production of the proinflammatory cytokines , such as tumor necrosis factor alpha ( TNF alpha ) , Interleukin 1beta ( IL 1beta ) , Interleukin 12 ( IL 12 ) , interferon gamma ( IFN gamma ) , as well as the inflammatory mediator nitric oxide ( NO ) , and concurrently enhanced the production of the anti inflammatory cytokine IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We used ELISPOT and cell ELISA to study secretion of IL 4 , IFN gamma , TGF beta , IL 6 , and TNF alpha by circulating mononuclear cells during the course of Guillain Barr syndrome ( GBS ) . ^^^ No significant differences were seen concerning the predominantly pro inflammatory cytokines IFN gamma , IL 6 or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Over expression of the IkappaBalpha serine mutant also inhibited reporter gene expression in response to IL 4 , TNF alpha , IL 1beta , and in some cases IFN gamma using constructs with sequences from the pIgR promoter . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Incubation of MIN 6 cells with IL 1beta , IFN gamma , and TNF alpha ( cytomix ) increased production of nitrites , with increased expression of iNOS mRNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Elevation of intracellular cAMP , on the other hand , potently suppressed macrophage TNF alpha production and modulated T cell response by inhibiting TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Sera levels of interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and interleukin 6 ( IL 6 ) were analyzed at different times after AdLacZ . ^^^ There was a strong correlation between the sera levels of IFN gamma , TNF alpha , and IL 6 , but cytokine responses were not significantly correlated with LacZ expression or the CTL response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OBJECTIVE : To assess the usefulness of interleukin 6 ( IL 6 ) , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) in the diagnosis and prediction of outcome of pleural tuberculosis . ^^^ PATIENTS AND METHODS : Pleural fluid from 32 TB and 34 non TB patients was sent for assay of IL 6 , TNF alpha and IFN gamma . ^^^ RESULTS : The pleural fluid Levels of IL 6 , TNF alpha and IFN gamma in TB patients were significantly higher than those with non TB effusions ( P values of < 0 . 001 , 0 . 018 and < 0 . 001 , respectively by independent t test ) . ^^^ The cut off values for IL 6 , TNF alpha and IFN gamma determined in this analysis were 4000 , 4 and 60 pg / ml respectively , and their sensitivity and specificity were 90 . 6 % and 76 . 5 % , 90 . 6 % and 79 . 4 % , 100 % and 100 % , respectively . ^^^ A negative correlation was found between the percentage reduction in pleural fluid cytokines after 2 weeks treatment and the extent of residual pleural scarring ( IL 6 : r= 0 . 62 , P=0 . 041 ; TNF alpha : r= 0 . 65 , P=0 . 030 ; IFN gamma : r=0 . 83 , P=0 . 002 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A complete cytokine mix ( CCM ) or its individual components tumour necrosis factor alpha ( TNF alpha ) , interleukin 1beta ( IL 1beta ) and interferon gamma ( IFN gamma ) were used to switch resting murine astrocytes to reactive states . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study , we investigated the type 1 / type 2 cytokine balance by measuring plasma concentrations of IL 2 , IL 4 , IL 5 , IL 10 , tumor necrosis factor ( TNF ) alpha and IFN gamma with microparticle based flow cytometric technology . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mangiferin has been reported to possess potent anti inflammatory property and we presume its presence in the aqueous extract of S . chirayita is responsible for reducing TNF alpha , IL 1beta , IL 6 , and IFN gamma and / or elevating IL 10 in the joint homogenates of arthritic mice on day 12 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effect of interleukin 1beta ( IL 1beta ) , tumor necrosis factor alpha ( TNF alpha ) , and gamma interferon ( IFN gamma ) on Trypanosoma cruzi multiplication and nitric oxide ( NO ) production in cardiac myocytes was investigated . ^^^ Cardiac myocyte cultures were obtained from neonatal Wistar rat hearts , infected with T . cruzi , and treated with IL 1beta , TNF alpha , IFN gamma , or N monomethyl L arginine ( L NAME ) for 72 h . ^^^ The addition of L NAME reversed the inhibitory effect on parasite growth of IL 1beta and TNF alpha but not of IFN gamma . ^^^ Nitrite levels released by T . cruzi infected and noninfected cardiac myocyte cultures after 72 h of stimulation with IL 1beta were significantly higher than those produced upon treatment with TNF alpha , IFN gamma , or medium alone , regardless of the infection status . ^^^ L NAME inhibited IL 1beta but not TNF alpha induced NO production , indicating the presence of iNOS dependent and iNOS independent mechanisms for NO formation in this experimental system . iNOS expression was detected in infected and noninfected cardiac myocytes stimulated with IL 1 beta and TNF alpha but not with IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inclusion of any of the costimulatory signaling regions in series with TCRzeta enhanced the level of specific Ag induced IL 2 , IFN gamma , TNF alpha , and GM CSF cytokine production and enabled resting primary T cells to survive and proliferate in response to Ag in the absence of any exogenous factors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Short term serum deprivation , suspension culture , and pretreatment with IFN gamma or TNF alpha increased , whereas long term serum free culture and pretreatment with TGF beta or IL 10 decreased the apoptotic susceptibility of DF . ^^^ Surface Fas expression was only modified by TNF alpha and IFN gamma , whereas all studied factors modified FLIP caspase 8 protein expression , consistently with their pro or antiapoptotic effects . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Few studies are available regarding the production of cytokines in the subclinical form of VL , with only the production of IFN gamma and TNF alpha known . ^^^ In the subclinical form of VL , variable levels of IL 2 were detected in 52 . 3 % of the children , IL 12 in 85 . 2 % , IFN gamma in 48 . 1 % , IL 10 in 88 . 9 % , and TNF alpha in 100 . 0 % , with the last two cytokines showing significantly lower levels than in the acute form . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Reductions in the proinflammatory cytokines IFN gamma and TNF alpha were non significant . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine production ( IL 2 , IL 4 , IL 5 , IL 10 , IL 12p40 , TNF alpha , and IFN gamma ) was assessed by cytometric bead array ( CBA ) and intracellular cytokine staining from alphaCD 3 antibody and mitogen stimulated peripheral blood mononuclear cells ( PBMCs ) from female RRMS and SPMS patients , and healthy controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OBJECTIVE : To determine the effect of the TH 2 environment with interferon gamma ( IFN gamma ) and heat shock protein 70 ( HSP 70 ) on CD 23 receptor expression and tumor necrosis factor alpha ( TNF alpha ) production . ^^^ Stimulating the CD 23 receptor with immune complexes significantly increased TNF alpha production by THP 1 cells stimulated with IFN gamma , IL 4 , IL 13 , or a combination of these . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In RIP LCMV mice , blockade of TNF alpha or IFN gamma in vivo abolished the prevention of T1D . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) production in culture supernatant was determined by enzyme linked immunosorbent assay . ^^^ Exogenous IL 15 further enhanced the production of IFN gamma , but not TNF alpha , of activated CB CD4+ T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
As immunomodulatory cytokines were shown to affect hair growth , we investigated the effects of IL 1beta , IL 4 , TNF alpha , TGF beta and IFN gamma on the expression levels of PDGF factors in cultured DPCs and FKs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Also in contrast to alphaGalCer , betaGalCer ( C 12 ) is a poor inducer of IFN gamma , TNF alpha , GM CSF , and IL 4 gene expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma primed , bone marrow derived macrophages from LRG 47 ( / ) and wild type animals produced equivalent levels of TNF and NO upon M . avium infection in vitro and developed similar intracellular bacterial loads . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
An enzyme linked immunosorbent assay was used to measure the production of the following cytokines : interleukin ( IL ) 1beta , IL 4 , IL 6 , IL 10 , IL 12 , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1R deficient islets were protected from the damaging effects of tumor necrosis factor ( TNF ) and interferon ( IFN ) gamma in vitro , and beta cell expression of iNOS was reduced , suggesting that IL 1 mediates the induction of iNOS by TNF and IFN gamma . ^^^ IL 1 action was not required for induction of class 1 major histocompatibility complex or Fas by TNF and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha and IFN gamma contents in supernatant and splenocyte proliferation were determined . ^^^ Terbutaline significantly suppressed IFN gamma and TNF alpha production by splenocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inhibition of the Janus kinase STAT 4 pathway by lovastatin modulated T 0 to Th 1 differentiation and reduced cytokine ( IFN gamma and TNF alpha ) production , thus inducing Th 2 cytokines ( IL 4 , IL 5 , and IL 10 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Periportal fibrosis in human Schistosoma mansoni infection is associated with low IL 10 , low IFN gamma , high TNF alpha , or low RANTES , depending on age and gender . ^^^ Whole blood samples were assayed for levels of nine cellular immune molecules ( IL 3 , IL 4 , IL 5 , IL 10 , IL 13 , TNF alpha , IFN gamma , IL 1beta , and RANTES ) in the absence of in vitro Ag stimulation , and after stimulation with egg and worm Ags . ^^^ Adult females at lowest risk had relatively high IFN gamma factor scores ( > 83rd percentile ) , whereas those at highest risk had a combination of intermediate ( 32nd to 83rd percentile ) IFN gamma and relatively high ( > 60th percentile ) TNF alpha factor scores . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increase in mRNA of IFN gamma , IL 2 , and TNF alpha was observed in peripheral blood mononuclear cells at the peak of infection and was seen to be related with pyrexia and parasitemia . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Unlike hBD 2 and hBD 3 mRNA was preferentially stimulated by IFN gamma rather than TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma / mice exhibited significant elevations in IL 6 , IL 10 , IL 12 , and tumor necrosis factor alpha in lesions compared to wild type mice , but these modulations had no net effect on IL 1alpha levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Heated CaP cells increased Th 1 cytokine ( IL 2 , IFN gamma , TNF alpha ) release but decreased Th 2 cytokine ( IL 4 , 5 , 10 ) release of TIL . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Colonic expression of collagens ( Col1a2 , Col3a2 ) , ECM remodeling genes ( matrix metalloproteinase [ MMP ] 1 , 3 , and tissue inhibitor of matrix metalloproteinase [ TIMP ] 1 ) , and inflammation modulating cytokines ( tumor necrosis factor alpha [ TNF alpha ] , interferon gamma [ IFN gamma ] , transforming growth factor beta 1 [ TGF beta 1 ] , and insulin like growth factor 1 [ IGF 1 ] ) were assessed by semiquantitative reverse transcription polymerase chain reaction . ^^^ Control and TNBS treated colonic mesenchymal cells were characterized by morphology , phenotype , and functional response to TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Anti proliferative effect of mycobacteria , IFN gamma and TNF alpha on primary cultures derived from endometrial stroma : possible relevance to endometriosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In C 6 astrocytoma cell line and primary astrocytes , 5 AzadC enhanced proinflammatory cytokine ( IFN gamma , TNF alpha , IL 1 ) triggered NO synthesis in a time and dose dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin 1 beta ( IL 1beta ) , tumor necrosis factor alpha ( TNFalpha ) and a mixture of IL 1 , TNF and epidermal growth factor ( EGF ) significantly increased the level of stromelysin 1 mRNA in mouse astrocytes while interferon gamma ( IFN gamma ) inhibited this response or was without effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum levels of TNF alpha , IL1beta and IFN gamma in EDU treated mice were significantly lower than those in vehicle treated mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Escherichia coli also produced significant mean increases of TNF alpha and IFN gamma compared with unstimulated control cells . ^^^ No significant increases in IL 1beta , TNF alpha or IFN gamma were observed with Lactobacillus plantarum in cells derived from normal or inflamed mucosa . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma induced SOCS 1 regulates STAT 6 dependent eotaxin production triggered by IL 4 and TNF alpha . ^^^ Western blotting analysis demonstrated that IFN gamma downmodulated STAT 6 phosphorylation induced by IL 4 and TNF alpha . ^^^ In SOCS 1 ( / ) MEF , IFN gamma inhibited neither STAT 6 phosphorylation nor eotaxin production induced by IL 4 and TNF alpha . ^^^ Conversely , retroviral transduction of SOCS 1 into MEF inhibited STAT 6 phosphorylation and eotaxin production induced by IL 4 and TNF alpha , in the absence of IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Concomitantly , Tc 1 and Tc 2 effector cell therapies potentiated endogenous recipient derived antitumor responses by inducing 1 ) local T cell derived chemokines associated with type 1 like immune responses ; 2 ) elevated levels of recipient derived OVA tetramer positive CD 8 memory T cells that were CD 44 ( high ) , CD 122 ( + ) , and Ly6C ( high ) that predominantly produced IFN gamma and TNF alpha ; and 3 ) heightened numbers of activated recipient derived Th 1 and Tc 1 T cell subpopulations expressing CD 25 ( + ) , CD 69 ( + ) , and CD 95 ( + ) cell surface activation markers . ^^^ Moreover , both Tc 2 and Tc 1 effector cell therapies were dependent in part on recipient derived IFN gamma and TNF alpha for long term survival and protection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Five days after injection , TS / A IL 21 tumors showed numerous infiltrating granulocytes , NK cells , and to a lesser extent CD 8 ( + ) T cells , along with the expression of TNF alpha , IFN gamma , and endothelial adhesion molecules ICAM 1 and VCAM 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The IFN gamma and TNF alpha production of PBL pulsed by a combination of IL 2 and IL 12 was significantly higher than those of PBL stimulated by either IL 2 or IL 12 alone . ^^^ The mRNA encoding perforin , granzyme B , as well as IFN gamma and TNF alpha , were markedly enhanced in PBL stimulated with a combination of IL 12 and IL 2 . ^^^ The pulsing procedure of IL 12 in combination with IL 2 resulted in the increase of IFN gamma and TNF alpha , and the expression of perforin and granzyme B mRNA in PBL obtained from HCC patients , as well as in those obtained from normal subjects . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both Tc 1 and Tc 2 effector cell subpopulations induced endogenous antitumor responses that were dependent , in part , on recipient derived IFN gamma and TNF alpha . ^^^ Collectively , tumor antigen specific Tc 1 and Tc 2 effector cell mediated therapy is initially dependent , in part , on effector cell derived FasL or LTalpha that may subsequently potentiate endogenous recipient derived type 1 antitumor responses dependent on TNF alpha and IFN gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Concentrations of interleukin ( IL ) 10 , IL 12 , IL 18 , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) were determined by commercially available enzyme linked immunosorbent assays ( ELISA ) in the SF from 48 patients with reactive arthritis , 33 with RA and 13 with osteoarthritis ( non inflammatory controls ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , while interleukin 12 and tumor necrosis factor a were dispensable for tumor suppression , in animals deficient in IFN gamma production or signaling , tumor growth and neovascularization were markedly enhanced . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Overproduction of TNF alpha by CD8+ type 1 cells and down regulation of IFN gamma production by CD4+ Th 1 cells contribute to toxic shock like syndrome in an animal model of fatal monocytotropic ehrlichiosis . ^^^ Lethal infections caused by high or low doses of IOE were accompanied by extensive liver damage , extremely elevated levels of TNF alpha in the serum , high frequency of Ehrlichia specific , TNF alpha producing CD 8 ( + ) T cells in the spleen , decreased Ehrlicha specific CD 4 ( + ) T cell proliferation , low IL 12 levels in the spleen , and a 40 fold decrease in the number of IFN gamma producing CD 4 ( + ) Th 1 cells . ^^^ Cross protection was associated with substantial expansion of IFN gamma producing CD 4 ( + ) and CD 8 ( + ) cells , but not TNF alpha producing CD 8 ( + ) T cells , a high titer of IgG2a , and a low serum level of TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expressions of TNF alpha and IFN gamma mRNA in spleens of tacrolimus treated D EMCV infected mice were lower than that of non treated tacrolimus DEMCV infected mice on 7 DPI . ^^^ The results suggest that tacrolimus suppresses expressions of TNF alpha and IFN gamma mRNAs to prevent the onset of D EMCV induced IDDM . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines such as IL 2 and IFN gamma enhance T cell expansion , induce cytotoxic T cells ( CTL ) and natural killer ( NK ) cell responses and prime additional mononuclear phagocytes to produce TNF alpha and IL 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin ( IL ) 2 , IL 12 , interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , IL 10 , and transforming growth factor beta 1 ( TGF beta 1 ) levels were determined by ELISA in spleen cell culture supernatants . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The resulting p 776 790 specific T cells responded readily to antigen by proliferating and producing type 1 cytokines ( IFN gamma and TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When lymphocytes were added to these cultures , IL 10 and TNF alpha elevation persisted and , in the patients with a systemic reaction , both IL 12 and IFN gamma were significantly reduced compared to PPD reactors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The levels of gamma interferon ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) and lnterleukin ( IL ) 10 were evaluated during pregnancy in sera of women chronically infected with Trypanosoma cruzi that delivered infected or non infected children . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To determine if such is the case in women with placental malaria , concentrations of interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 4 ( IL 4 ) , and IL 10 were measured in placental plasma of 391 malaria infected and uninfected Cameroonian women with premature and full term deliveries . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
On the other hand , IFN gamma could stimulate Mphis to limit amastigote replication when it was coupled with lipopolysaccharides but not when it was coupled with tumor necrosis factor alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These T cell proliferation responses were associated with intracellular expression of interleukin 2 ( IL 2 ) and secretion of interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When supernatants of RJ suspensions were added to a culture of mouse peritoneal macrophages stimulated with lipopolysaccharide and IFN gamma , the production of proinflammatory cytokines , such as TNF alpha , IL 6 , and IL 1 , was efficiently inhibited in a dose dependent manner without having cytotoxic effects on macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The proinflammatory cytokines Tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) may be responsible for the immune mediated pathology observed in some patients . ^^^ We investigated the response of CD34+ cells from aplastic anemia patients to a combination of IFN gamma and TNF alpha , and compared them to cells from normal volunteer donors . ^^^ This was to determine whether aplastic CD34+ cells are more sensitive than normal cells to IFN gamma / TNF alpha mediated effects , and whether cytokine induced CD 95 expression can explain the high levels of apoptosis observed in CD34+ cells from aplastic patients . ^^^ IFN gamma / TNF alpha induced upregulation of CD 95 on both normal and aplastic CD34+ cells , although the basal level of CD 95 expression was increased in aplastic cells . ^^^ We conclude that increased apoptosis in aplastic CD34+ cells is not due to increased sensitivity to IFN gamma / TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : CD4+ T cells from MGS significantly expressed mRNA of interferon ( IFN ) gamma , interleukin ( IL ) 2 , granulocyte macrophage colony stimulating factor ( GM CSF ) , transforming growth factor ( TGF ) beta , tumor necrosis factor ( TNF ) alpha , IL 4 , CD 40 ligand ( CD40L ) , Fas ligand ( FasL ) and IL 2 receptor ( IL 2R ) during dry period , and mRNA of IFN gamma , IL 2 and TGF beta during lactation period . ^^^ CD8+ T cells from MGS substantially expressed mRNA of IFN gamma , IL 2 , GM CSF , TGF beta , TNF alpha , FasL and IL 2R during dry period and mRNA of IFN gamma , GM CSF , TGF beta , TNF alpha and c kit during lactation period . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we sought to determine if prolactin ( PRL ) had any influence on burn induced alterations in myelopoiesis and serum IL 6 , IL 10 , IL 12 , IFN gamma , TNF alpha , and MCP 1 levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Depletion of CD 4 and CD 8 T cells decreased IFN gamma levels , prevented granuloma and tissue necrosis , and prolonged the survival of TNF ( / ) hosts . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The induction of tumor specific reactive T cells in the nodes and spleens , as measured by IFN gamma production , was highest with IL 12 and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Resistance against H2O2 , NO , human Il 1beta , IFN gamma , or TNF alpha was assessed by trypan blue exclusion and insulin determination . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Surprisingly , vigorous HIV replication of CXCR 4 and CCR 5 tropic HIV strains did not result in characteristic mRNA profiles for IL 1beta , 2 , 4 , 6 , 8 , 10 , 12 , 15 , IFN gamma , TNF alpha , TGF beta , and beta chemokines in tissue blocks or HLAC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although corneal endothelial cells were unable to express MHC class 2 in response to IFN gamma alone , these cells readily expressed MHC class 2 molecules via a CIITA independent pathway when triggered by simultaneous exposure to IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Ang 2 alone increased OPN mRNA ( 3 . 3 + / 0 . 3 folds ; P < 0 . 05 ; n = 7 ) , while interleukin 1beta ( IL 1beta ) , tumor necrosis factor ( TNF alpha ) , and interferon gamma ( IFN gamma ) had no effect . ^^^ A combination of Ang 2 with IL 1beta or TNF alpha , not IFN gamma , increased OPN mRNA more than Ang 2 alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After the grafts survived for 4 to 8 weeks , immunohistochemistry and ELISA methods were employed to determine the contents of IL 1 beta , IL 4 , IL 6 , TNF alpha and IFN gamma in the exudation fluid from wounds after the rejection of xeno ADM , local skin and peripheral blood . ^^^ RESULTS : It was exhibited by immunochemistry staining that the positive cellular density and staining intensity of the IL 1 beta , IL 4 , IL 6 , TNF alpha and IFN gamma in the grafts were ranked as following : Xeno ADM > allo ADM > auto TTS ( P < 0 . 05 0 . 01 ) . ^^^ The levels of IL 4 , IL 6 , TNF alpha and IFN gamma in the exudation fluid during the rejection of xeno ADM were obviously higher than those in the blood of the patients as determined by ELISA , while the serum levels of IL 4 and IFN gamma in xeno ADM group were lower and higher than those in xeno ADM when without rejection respectively . ^^^ The serum levels of IL 4 , TNF alpha and IFN gamma in xeno ADM group were significantly higher than those in allo ADM and auto TTS groups ( P < 0 . 05 0 . 01 ) . ^^^ CONCLUSION : Local detection of high levels of IL 1 beta , IL 4 , IL 6 , TNF alpha and IFN gamma might be related to the immune augmentation mediated by cytotoxic lymphocytes and cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effect of IFN gamma and TNF alpha on the eosinophilic differentiation and NADPH oxidase activation of human HL 60 clone 15 cells . ^^^ The aim of this study was to investigate the effect of interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) on NADPH oxidase activity and gp 91 phox gene expression in HL 60 clone 15 cells as they differentiate along the eosinophilic lineage . ^^^ IFN gamma ( 100 U / ml ) and TNF alpha ( 1000 U / ml ) or IL 5 ( 200 pM ) caused a significant increase in the expression of the eosinophil peroxidase ( EPO ) and the major basic protein ( MBP ) genes . ^^^ IFN gamma ( 100 U / ml ) and TNF alpha ( 1000 U / ml ) also caused a significant increase in superoxide release by HL 60 clone 15 cells after 2 days compared with control or with butyric acid induced cells . ^^^ HL 60 clone 15 cells cultured with IFN gamma and TNF alpha for 2 days showed a significant increase in gp 91 phox gene expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The IL 1 , TNF alpha and IFN gamma were found in supernatant of OFs treated by patients ' serum . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We measured TNF alpha , TxB 2 , and IFN gamma production and the phosphorylation of p 38 , JNK , and ERK 1 / 2 in human promonocytic THP 1 cells . ^^^ In contrast to TNF alpha and TxB 2 production , HKSa induced IFN gamma was up regulated ( 26 + / 5 % ) in HKSa pretreated cells compared with HKSa stimulated naive cells . ^^^ IFN gamma antibody exhibited reversed priming in HKSa pretreated cells as evidenced by a reduction in TNF alpha . ^^^ In contrast , exogenous IFN gamma pretreatment prevented the induction of LPS homologous tolerance resulting in an increase in TNF alpha production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To understand the cumulative effect of these factors in AA amyloidosis , humoral levels of SAA , IFN gamma , tumour necrosis factor ( TNF alpha ) and granulocyte macrophage colony stimulating factor were determined in azocasein ( AZC ) treated CD 1 mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Proliferation and production of interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) by CD1d restricted T cells , in the presence of B CLL cells loaded with alpha GalCer , were also observed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although melatonin administration raises serum levels of tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) , the mortality observed in neutralization experiments with the corresponding anticytokine antibodies , suggests that neither TNF alpha nor IFN gamma are essential for the protective effect of melatonin on murine VEE virus infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Most lymphocytes in the lamina propria of oral lichen planus ( OLP ) lesions express and secrete interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) , whereas they do not secret interleukin 4 and 10 or transforming growth factor beta . ^^^ In conclusion , we suggest that genetic polymorphism of the first intron of the promoter gene of IFN gamma may be an important risk factor to develop oral lesions of LP , whereas an increase in the frequency of 308A TNF alpha allele may best contribute to the development of additional skin involvement . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pulmonary responses 24 h postexposure to LPS + IFN gamma were characterized by significantly decreased TAC , increased BAL AMs and PMNs , LDH , albumin , TNF alpha , and MIP 2 , and enhanced AM CL to the same extent in both WT and iNOS KO mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast to LPS , stimulation of PBMC with live Borrelia , increases also the production of IL 12 and IFN gamma , besides IL 1beta , TNF alpha , IL 6 and IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Newly polarized Th 1 cells and IFN gamma expressing Th 1 cells , both of which express IL 18 receptor alpha chain strongly , produce IFN gamma , IL 9 , IL 13 , granulocyte / macrophage colony stimulating factor , tumor necrosis factor alpha , regulated on activation , normal T cell expressed and secreted , and macrophage inflammatory protein 1alpha upon stimulation with Ag , IL 2 , and IL 18 in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The following immunological and neuroendocrine parameters have been analysed : peripheral white blood cells count , CD11b integrin expression and H ( 2 ) O ( 2 ) production by neutrophils , lymphocyte and monocyte phenotype , intracytoplasmic cytokine ( IFN gamma , TNF alpha and IL 4 ) pattern , lymphocyte proliferation to mitogens and antigens , cortisol , ACTH , catecholamines , GH , LH , prolactin and testosterone plasma levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We determined the extent of expression of three cytokines ( IFN gamma , IL 4 , and TNF alpha ) in brain tissue infected with human immunodeficiency virus 1 ( HIV 1 ) . ^^^ The selections were IFN gamma as a Th 1 cytokine , IL 4 as a Th 2 cytokine , and TNF alpha as a pro inflammatory cytokine ( and because of its prior implication in brain tissue damage due to HIV 1 infection ) . ^^^ Post mortem temporal lobe tissue specimens from 16 HIV 1 seropositive patients and 11 HIV 1 seronegative controls were stained for IFN gamma , IL 4 , and TNF alpha utilizing immunohistochemistry and alkaline phosphatase . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RNA from pleural effusion cells was examined to determine TGF beta 1 , TNF alpha , IFN gamma , and interleukin 8 mRNA levels by using real time PCR . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To do this , we examined expression of genes encoding interleukin 1alpha ( IL 1alpha ) , IL 2 , IL 4 , IL 5 , IL 6 , IL 8 , IL 10 , IL 12p35 , IL 16 , and IL 18 , as well as genes encoding gamma interferon ( IFN gamma ) , transforming growth factor beta ( TGF beta ) , and tumor necrosis factor alpha ( TNF alpha ) , in PBMCs , intestinal lesions , and mesenteric lymph nodes of cattle naturally infected with M . avium subsp . paratuberculosis . ^^^ Our comprehensive results demonstrate that for most cytokine genes , including the genes encoding IFN gamma , TGF beta , TNF alpha , IL 1alpha , IL 4 , IL 6 , IL 8 , and IL 12p35 , differential expression in PBMCs from infected and control cattle did not require stimulation with M . avium subsp . paratuberculosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These cells produce IFN gamma and TNF alpha , and a subset also produces IL 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , toxin A induced gene expression of TNF alpha , neutrophil chemotactic chemokines , and ICMA 1 was remarkably attenuated in IFN gamma KO mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Long term suppression of tumor growth by TNF requires a Stat 1 and IFN regulatory factor 1 dependent IFN gamma pathway but not IL 12 or IL 18 . ^^^ These results strongly suggest a novel function for the endogenous IFN gamma pathway that without measurable IFN gamma production or activity affects the ability of TNF to suppress tumor development . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
LEW into WF . 1L kidney grafts developed proteinuria starting 120 d after transplantation and were associated with morphologic changes of focal segmental glomerulosclerosis together with interstitial cell infiltrates , upregulated gene expression of IL 1beta , IL 2 , and TNF alpha / beta , as well as IL 2 , IFN gamma , and TNF alpha production by lymphocytes in MLR culture supernatants . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The results show that Ti dioxide is not immunotoxic ; Ti oxalate is strongly immunotoxic ; titanocene inhibits cytokine release but not PBMC proliferation , while Ti ascorbate inhibits TNF alpha release from PBMC but not that of IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Te ( 4 ) inhibited PBMC proliferation and IFN gamma , IL 5 and TNF alpha release from PBMC more than Te ( 6 ) . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Proinflammatory cytokines , such as tumor necrosis factor alpha ( TNF alpha ) , interleukin 1beta ( IL 1beta ) , IL 6 , and interferon gamma ( IFN gamma ) are produced by macrophages treated with ginseng radix extract ( GRE ) in vitro as well as in vivo . ^^^ In the present study , we demonstrated that production of TNF alpha and IFN gamma was induced by GRE in spleen cells and peritoneal macrophages from C3H / HeN mice but was impaired in C3H / HeJ mice carrying a defective toll like receptor 4 ( TLR 4 ) gene . ^^^ However , GRE induced production of TNF alpha and IFN gamma in the presence of polymyxin B , an LPS inhibitor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , the treatment of DNA vaccinated CD 4 ( / ) mice with an anti CD 8 or anti gamma interferon ( IFN gamma ) antibody significantly reduced the effect of immunization , and neither IFN gamma ( / ) nor tumor necrosis factor receptor deficient mice were protected by DNA immunization ; therefore , the primary vaccine induced protective mechanism in these immune deficient mice likely involves the secretion of cytokines from activated CD 8 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our results indicate that OPN up regulates the production of IL 12 and IFN gamma within the granulomas at early stages , and OPN has an additional role in the regulation of cellular recruitment and TNF alpha production at late stages that determine the severity of liver tissue injury . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The TNF alpha and IFN gamma secretion and cytotoxicity of TIL against HepG 2 liver cancer cells were estimated by ELISA and MTT , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
I . p . administration of pMP6 / liposome complexes resulted in the expression of the proinflammatory cytokines IL 6 , IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Type 1 IFNs may either prime cells for stronger responses to viruses , bacterial pathogens and cytokines such as IL 6 and IFN gamma , or may suppress cellular responses to LPS and TNFalpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : During routine passage , less than 5 % of cells were CD8a+ , although 20 40 % of cells expressed CD8a when treated with IL 2 + TGF beta , whereas TPA + Calcium ionophore , IFN gamma , and TNF alpha cause no significant changes in the proportion of CD8+ cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OBJECTIVE : The purpose of the present study was to assess the relation between clinical parameters and concentrations of the key ( IL 1beta , TNF alpha , IL 2 , IFN gamma , IL 4 , IL 10 ) cytokines , important in the initiation and progression of periodontal diseases , within inflamed gingival tissues and serum samples from patients with severe chronic periodontitis . ^^^ RESULTS : The concentrations of IL 1beta , TNF alpha , IL 2 , IFN gamma were , on average , significantly higher in serum samples and gingival tissue biopsies from periodontitis patients than in healthy controls . ^^^ High concentrations of TNF alpha , IFN gamma and IL 2 and , especially , a high ratio of IL 1beta / IL 10 and TNF alpha / IL 4 found in tissue biopsies from periodontitis patients , strongly correlated with the severity of periodontitis . ^^^ In contrast , high absolute levels of IL 1beta , TNF alpha , IL 2 and IFN gamma and , especially their high ratios to IL 4 and IL 10 found in inflamed tissue biopsies , were closely associated with periodontal disease severity . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The mRNA expression of all CXCR 3 ligands was induced in synovial fibroblasts from RA patients after stimulation with interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , or interleukin 1 beta ( IL 1beta ) . ^^^ However , synovial fibroblasts significantly secreted CXCL 9 and CXCL 10 proteins , but not CXCL 11 protein , after IFN gamma stimulation and secreted only CXCL 10 protein after TNF alpha or IL 1beta stimulation . ^^^ When stimulated with a combination of IFN gamma and TNF alpha , these cells were able to secrete large amounts of all three chemokines . ^^^ These results indicate that synovial fibroblasts may be involved in perpetuating the Th 1 immune response by producing the Th 1 associated CXCR 3 ligands , and the synergistic effect of IFN gamma and TNF alpha may be important for their chemokine production in RA joints . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In IHC , TNF alpha , IFN gamma and IL 2 were immunostained dominantly in the mesangial region , but not in the tubulointerstitial region . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : The levels of IL 15 , TNF alpha , IFN gamma , and IL 17 were all significantly increased in the serum of RA patients compared with normal control serum . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cells were stained for T cell surface markers CD 4 and CD 8 and for the intracellular cytokines interferon gamma ( IFN gamma ) , TNF alpha , interleukin 4 ( IL 4 ) , and IL 10 . ^^^ RESULTS : Compared with baseline , infliximab treatment induced a significant decrease at 12 weeks in the number of CD4+ and CD8+ T cells that were positive for IFN gamma and TNF alpha upon PMA / ionomycin stimulation ( P = 0 . 005 ) . ^^^ No change in the percent IFN gamma or TNF alpha positivity among CD4+ and CD8+ subpopulations was observed after 6 weeks in patients treated with placebo . ^^^ However , when these patients began infliximab treatment after 6 weeks of receiving placebo , there was a similar significant decrease in IFN gamma and TNF alpha production by CD4+ and CD8+ T cells ( P < 0 . 05 ) . ^^^ Between group analysis showed that the change in the expression of IFN gamma and TNF alpha in both CD4+ and CD8+ T cells was significantly different between the infliximab and placebo groups ( P = 0 . 001 for all variables ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cyclooxygenase ( COX ) 2 expression and activity in response to pro inflammatory cytokines TNF alpha and IFN gamma was evaluated in the colonic epithelial cell line HT 29 and the airway epithelial cell line A 549 . 2 . ^^^ Co stimulation of TNF alpha with IFN gamma resulted in reduced COX 2 mRNA and protein expression . 4 . ^^^ IFN gamma had no effect on the stability of TNF alpha induced COX 2 mRNA . 5 . ^^^ TNF alpha induced PGE ( 2 ) biosynthesis was significantly enhanced by the simultaneous addition of IFN gamma and was COX 2 dependent . 6 . ^^^ The combination of IFN gamma and TNF alpha induced the microsomal prostaglandin E synthase ( mPGES ) , comensurate with the enhanced PGE ( 2 ) synthesis . 7 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the effect of IFN gamma or IFN gamma / IFN alpha and IFN gamma / TNF treatment on the apoptosis of Ewing tumor cells ( SK N MC ) . ^^^ The results show that treatment of cells with IFN gamma resulted in 17 % of cell death while no effect was observed when cells were treated with IFN alpha or TNF alone . ^^^ Percentage cell death increased markedly in IFN gamma / IFN alpha and IFN gamma / TNF treated cells ( 42 % and 67 % , respectively ) as compared to IFN gamma treated cells . ^^^ These results suggest that IFN alpha and TNF amplified the apoptotic signal ( s ) induced by IFN gamma . ^^^ The results reported here are consistent with the notion that cell associated and secreted TRAIL contribute in an autocrine or paracrine manner to the triggering of Ewing cell apoptosis induced by IFN gamma alone or combined with IFN alpha or TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The CD 8 ( + ) T cells also up regulated mRNAs for cytokines ( IFN gamma and TNF alpha ) and C C chemokines ( RANTES and MIP 1alpha ) , which have a major role in immunity against the pathogen . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma levels were significantly higher in CD and CD IgAD than in controls , TNF alpha displayed significantly higher levels in CD IgAD when compared both with controls and with CD , and IL 2 was in CD IgAD significantly increased respect to controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytotoxicity assays showed that TNF alpha but not IFN gamma was toxic to the neural stem cells under proliferative conditions . ^^^ Here we examine the effects of two inflammatory cytokines , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNFalpha ) , on adult neural stem cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Proinflammatory cytokines TNF alpha and IFN gamma alter laminin expression under an apoptosis independent mechanism in human intestinal epithelial cells . ^^^ The effect of TNF alpha , IFN gamma , IL 1beta , IL 6 , and transforming growth factor ( TGF ) beta was analyzed on the expression of laminins in the normal human intestinal epithelial crypt ( HIEC ) cell line . ^^^ However , in combination , TNF alpha and IFN gamma synergistically stimulated the secretion of both laminin 5 and 10 in HIEC cells . ^^^ Interestingly , the TNF alpha and IFN gamma combination was also found to significantly promote apoptosis . ^^^ These results demonstrate that laminin production is specifically modulated by the proinflammatory cytokines TNF alpha and IFN gamma in intestinal epithelial cells under an apoptosis independent mechanism . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Correlations between local expression of insulin like growth factor 1 , insulin like growth factor receptor , epithelial growth factor , transforming growth beta 2 factor , PCNA , TNF alpha , TNF receptor 1 , Fas , FasL , IFN gamma , IL 1beta , IL 4 , IL 6 , IL 8 , IL 10 , and IL 12 genes in intact and hyperplastic endometrium and in the endometrium after hormone therapy were analyzed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When compared with the original material , CLN obtained by this method shows ( 1 ) similar pattern of peptides in SDS PAGE , ( 2 ) identical amino acid analysis , characterized by high content of proline ( 22 % ) , a high proportion of nonpolar amino acids , a low percentage of glycine , alanine , arginine , histidine , and no tryptophan , methionine , and cysteine residues , ( 3 ) similar pattern of HPLC profiles , and ( 4 ) its ability to induce IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 2R , IFN gamma , TNF alpha , IL 1 and specific IgG subclasses were determined by ELISA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lewis lung tumor cells ( 3LL cells ) were also incubated with a cytokine mixture ( IL 1 beta , IFN gamma , and TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Myocytes and muscle strips were stimulated with 10 ng / ml of IL 1beta , TNF alpha , and IFN gamma , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The aim of this study was to investigate the effects of TNF alpha and IFN gamma on intestinal Pgp expression , activity , and localization in Caco 2 cells grown on filters . ^^^ Associated with TNF alpha , IFN gamma produced neither an antagonist nor synergistic effect on Pgp activity . ^^^ In conclusion , our results demonstrate an inhibitory effect of TNF alpha and no effect of IFN gamma on Pgp transport activity using rhodamine 123 as a substrate . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The aim of the present study was to investigate the effects of ( 1 ) the pro inflammatory cytokines IL ( interleukin ) 1beta , TNF alpha ( tumour necrosis factor alpha ) , IFN gamma ( interferon gamma ) and anti inflammatory cytokines IL 4 and IL 13 , and ( 2 ) NO ( nitric oxide ) donors on HA ( hyaluronic acid ) production by synovial cells from patients with rheumatoid arthritis . ^^^ Synovial cells obtained from five patients with rheumatoid arthritis were incubated for 24 h without or with IL 1beta , TNF alpha , IFN gamma , or with this mixture for 24 h plus IL 4 or IL 13 for the last 6 h . ^^^ IL 1beta , TNF alpha and IFN gamma alone or in combination stimulated HA synthesis , whereas IL 4 and IL 13 dose dependently inhibited HA production induced by Th 1 cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here , we show that within the effector memory Vdelta 2 population , 2 distinct and complementary subsets with regard to phenotype , mode of activation , and type of responses can be identified : Vdelta 2 T ( EMh ) cells , which express high levels of chemokine receptors , but low levels of perforin and of natural killer receptors ( NKRs ) and which produce large amounts of interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) in response to T cell receptor ( TCR ) specific stimulation by phosphoantigens ; and Vdelta2T ( EMRA ) cells , which constitutively express several NKRs , high amounts of perforin , but low levels of chemokine receptors and of IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , eosinophils sometimes accumulate during disease with expression of Th 1 cytokines [ i . e . , interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and IL 1beta ] . ^^^ TNF alpha but not IL 1beta enhanced the IFN gamma induced production of MIG and IP 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , iNOS expression was detectable by RT PCR in all batches of astrocytes tested when stimulated with a cytokine cocktail of IFN gamma , TNF alpha , IL 1 and LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In combination , NM inhibited up regulation of MHC 2 by IFN gamma while priming enhanced release of tumor necrosis factor alpha and nitric oxide . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , circulating levels of interferon gamma ( IFN gamma ) , interleukin 10 ( IL 10 ) , and soluble tumor necrosis factor receptor 2 ( sTNF RII ) , 3 parameters associated with schistosomiasis morbidity , were significantly increased in co infected children . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Exposure of RINm5F cells to IL 1beta or to a cytokine mixture ( IL 1beta , TNF alpha , IFN gamma ) for 6 h resulted in the differential expression of a functional gene cluster . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Quantification of IL 2 , IL 4 , IL 5 , IFN gamma , and TNF alpha by cytometric bead array ( CBA ) assay determined the polarization of TH1 / TH2 responses , whereas H 2 Kb / SIINFEKL tetramers monitored the expansion of OVA specific T cells . ^^^ The hybrids also induced the most potent CTLs , offered the highest protection against established EG 7 tumors and also induced the highest stimulation of IFN gamma and TNF alpha production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The serum concentrations of interleukin 1 ( IL 1 ) , IL 4 , IL 6 , IL 8 , IL 10 , tumor growth factor beta ( TGF beta ) , tumor necrosis factor alpha ( TNF alpha ) , and gamma interferon ( IFN gamma ) were measured by enzyme linked immunosorbent assays ( ELISA ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although no statistical differences in mortality and parasitaemia were found , conventional mice showed IFN gamma , TNF alpha and NO production ( P < 0 . 05 ) by spleen cell cultures and higher blood levels of immunoglobulins of the IgG2a isotype ( P < 0 . 05 ) when compared to their germ free counterparts . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Production of tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) and interleukin ( IL ) 10 was measured by ELISA , while the percentages of TNF alpha , IFN gamma and IL 4 positive CD3+ cells were determined in the same groups of patients and healthy subjects using flow cytometry . ^^^ CONCLUSIONS : High production of TNF alpha and IFN gamma detected in ChrReA supports the possible use of anti TNF alpha treatment in ChrReA . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In leprosy patients with the TLR 2 mutation , production of IL 2 , IL 12 , IFN gamma , and TNF alpha by M . leprae stimulated PBMC were significantly decreased compared with that in groups with wild type TLR 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The immunological response in HTLV 1 infected individuals is characterized by a prominent Type 1 cytokine response with high production of IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Notably , the number of IL 1 and IL 8 producing monocytes as well as TNF alpha and IFN gamma producing lymphocytes were not significantly affected by 20 mM vitamin C . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Short term stimulation ( i . e . < 2 days ) with tumor necrosis factor alpha ( TNF alpha ) or interferon gamma ( IFN gamma ) cause growth arrest and sensitize epithelial cells to CD 95 ( Fas / Apo 1 ) mediated cell death . ^^^ In the present study HT 29 and DLD 1 colonic cells were stimulated with either TNF alpha or IFN gamma at varying concentrations for 2 9 days . ^^^ Cells stimulated for 8 days with IFN gamma ( 10 pM ) or TNF alpha ( 100 pM ) had higher proliferation rates than controls or cells stimulated for 2 days ( p < 0 . 05 ) . ^^^ Colonic cells continuously exposed to IFN gamma or TNF alpha had cell turnover characteristics that resemble findings in patients with UC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Testicular cytokines and growth factors ( such as IL 1 , IL 6 , TNF , IFN gamma , LIF and SCF ) were shown to affect both the germ cell proliferation and the Leydig and Sertoli cells functions and secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here , we show that tyrosyl radicals generated by human neutrophils after activation by phorbol 12 myristate 13 acetate ( PMA ) , interferon gamma ( IFN gamma ) or TNF alpha could act in an autocrine manner by cross linking to endogenous proteins . ^^^ Although all three stimuli induced tyrosylation of the filamentous component vimentin , additional tyrosylated vimentin fragments were detected after IFN gamma and TNF alpha stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Outcome variables included bronchoalveolar lavage ( BAL ) for M . pneumoniae quantitative culture and cytokine and chemokine concentration determinations by enzyme linked immunosorbent assay ( tumor necrosis factor alpha [ TNF alpha ] , gamma interferon [ IFN gamma ] , interleukin 1beta [ IL 1beta ] , IL 2 , IL 4 , IL 12 , granulocyte macrophage colony stimulating factor , IL 8 , monocyte chemoattractant protein 1 [ MCP 1 ] , and macrophage inflammatory protein 1alpha [ MIP 1alpha ] ) , histopathologic score of the lungs ( HPS ) , and pulmonary function tests ( PFT ) using whole body , unrestrained plethysmography at the baseline and post methacholine exposure as indicators of airway obstruction ( AO ) and airway hyperresponsiveness ( AHR ) , respectively . ^^^ Cytokine concentrations in BAL samples were reduced in mice that received cethromycin , and the differences were statistically significant for 7 of the 10 cytokines measured ( TNF alpha , IFN gamma , IL 1beta , IL 8 , IL 12 , MCP 1 , and MIP 1alpha ) on day 4 ( P < 0 . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
With the purpose of understanding the immunological mechanisms involved in this protection , the lymphoproliferative response , IFN gamma and other cytokines like interleukin ( IL 5 , IL 10 ) , and tumor necrosis factor alpha ( TNF alpha ) were evaluated before and after the use of anti TB drugs on 30 patients with active TB disease , 24 healthy household contacts of active TB patients , with positive purified protein derivative ( PPD ) skin tests ( induration > 10 mm ) , and 34 asymptomatic individuals with negative PPD skin test results ( induration < 5 mm ) . ^^^ The positive lymphoproliferative response among peripheral blood mononuclear cells of patients showed high levels of IFN gamma , TNF alpha , and IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma , TNF alpha , IL 4 and IL 10 secreting T cells were intracytoplasmic evaluated in an attempt to identify M . leprae and PPD specific cells directly from the peripheral blood . ^^^ The analysis by this approach indicated that TNF alpha was the first ( 8 h ) to be produced , followed by IFN gamma ( 16 h ) , IL 10 ( 20 h ) and IL 4 ( 24 h ) , and double staining experiments confirmed that CD4+ were a greater source of TNF alpha than of CD8+ T cells ( P < 0 . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The adjuvant use of recombinant bovine cytokines , such as IL 2 , IFN gamma and TNF alpha , in normal mammary gland , mobilizes innate and acquired immunity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Highly neurovirulent SFV 4 effectively induced IFN gamma , TNF alpha , IL 6 and TGF beta , but its avirulent derivative V 4 opal with nsP 3 arginine 476 to opal mutation , elicited only weak induction of these cytokines . ^^^ In contrast , structurally identical but moderately virulent rA 774 arg virus with sense codon at opal position in nsP 3 , markedly stimulated synthesis of IFN gamma , TNF alpha , and IL 10 transcripts , without , however , reaching the levels elicited by lethal SFV 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inducible nitric oxide synthase ( iNOS ) is transcriptionally under the control of IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN alpha , IFN gamma , suppressor of cytokine signaling ( SOCS ) , TNF alpha and others ] and pathogen associated molecular patterns [ PAMPs , e . g . lipopolysaccharide ( LPS ) and CpG DNA ] in HCV disease ; this information might guide future therapies aimed at improving IFN alpha responsiveness . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Patients who persistently fail to respond , however , express abnormally raised levels of the pro inflammatory cytokines tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) , which are also known to inhibit erythropoiesis . ^^^ Preliminary results suggest that once daily treatment with 400 mg of pentoxifylline orally not only can reduce T cell expression of TNF alpha and IFN gamma , but can also restore the response to erythropoietin and improve haemoglobin levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In myotubes and mouse muscles , TNF alpha plus IFN gamma strongly reduced myosin expression through an RNA dependent mechanism . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We determined IL 6 and TNF alpha production in peripheral blood cultured with LPS , as well as IFN gamma and TGF beta induced by PHA stimulation . ^^^ We observed significantly higher levels of IFN gamma ( 355 . 8 pg / ml versus 98 . 0 pg / ml ; P = 0 . 01 ) and a trend toward increased TNF alpha production ( 2410 . 2 pg / ml versus1980 . 2 pg / ml ; P = 0 . 07 ) in RPL women as compared to controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immature 16+mDC and 16 mDC ( iDC ) obtained by culture of purified monocytes with GM CSF and IL 4 were maturated with ( 1 ) Toll like receptor ( TLR ) ligands [ lipopolysaccharide ( LPS ) ] , ( 2 ) lymphocyte derived ( soluble CD 40 ligand , IFN gamma ) and ( 3 ) endogenous inflammatory stimuli [ TNF alpha , prostaglandin ( PG ) E 2 ] . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 2 , IL 4 , IL 6 , IL 10 , IFN gamma , TNFalpha , TGFbeta , MCP 1 , RANTES , perforin , IP 10 , FasL , FasR and MIP lalpha were determined . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and interleukin 6 ( IL 6 ) responses were detected during reactions to most antigens tested , while IL 4 responses were absent . ^^^ Cytokine analysis in arthritic joints revealed consistent expression of IL 1 , IL 4 , IL 6 , TNF alpha , and IFN gamma mRNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Membrane fraction of mouse myeloma Ag 8 cells that was transfected with human CD 154 ( Ag 8 hCD154 ) induced production of TNF alpha , IL 6 , and MMP 3 in RA chondrocytes , and pretreatment of RA chondrocytes with IFN gamma enhanced the response . ^^^ OA chondrocytes did not respond to stimulation with the membrane fraction of Ag 8 hCD154 cells alone , but the cells did produce TNF alpha , IL 6 , and MMP 3 after pretreatment with IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
As confirmed by reverse transcription polymerase chain reaction ( RT PCR ) , loss of IFN gamma results in down regulation of tumor necrosis factor alpha ( TNF alpha ) and tissue inhibitor of matrix metalloproteinase 1 ( TIMP 1 ) while up regulating expression of vascular endothelial growth factor ( VEGF ) and tenascin C . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The first reaction type , i . e . production of IFN alpha , IFN gamma , IL 1beta , IL 6 and TNFalpha but not of IL 2 or IL 4 , denotes the primary body reaction to an infectious agent . ^^^ The third reaction type is associated with production of IFN alpha , IFN gamma , IL beta , IL 6 and TNFalpha by blood leucocytes affected by HSV 1 infected leucocytes . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we explored the effect of the MLK inhibitors CEP 1347 and CEP 11004 on the activation of murine astrocytes by either TNF plus IL 1 or by a complete cytokine mix containing additional IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , IL 6 , and IL 10 were measured in homogenates of fetal brain and placenta at serial time periods within the first 24 h after the inflammatory stimulus . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pro inflammatory cytokines ( tumor necrosis factor alpha TNF alpha , IL 1 , IL 8 , IL 12 , IFN gamma , etc . ) represent a part of this immuno inflammatory response to an insult . ^^^ The results of the clinical investigation of correlation between pro inflammatory cytokines ( IL 8 , IL 12 , TNF alpha , IFN gamma ) , the outcome ( survivors , non survivors ) , and the severity ( systemic inflammatory response syndrome SIRS less severe , and MODS more severe ) in polytraumatised patients with sepsis are presented in this paper . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Breast milk was collected 3 days post partum and fatty acids were analysed by gas liquid chromatography and IgA , sCD 14 and cytokines ( IL 5 , IL 6 , IL 10 , TNF alpha and IFN gamma ) were quantitated by ELISA or time resolved fluorescence ( TRF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cell free supernatants were obtained and evaluated for interferon gamma ( IFN gamma ) , interleukin ( IL ) 12 ( p 40 ) , tumor necrosis factor alpha , IL 6 , IL 10 , IFN alpha , IL 8 , and IL 1beta by ELISA . ^^^ RESULTS : Compared to LPS alone , significantly reduced levels of IFN gamma , IL 12 ( p 40 ) , tumor necrosis factor alpha , and IL 6 were associated with both CpG and GpC ODNs administration to LPS stimulated whole blood . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Reduced levels of IFN gamma and IL 18 binding protein ( IL 18BP ) were observed in the colon of IRF 1 KO mice , whereas levels of inducible nitric oxide synthase , cyclooxygenase 2 , phosphorylated STAT 3 , chemokines , TNF alpha , IL 1beta , IL 15 , and IL 18 were not significantly changed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The polymorphisms of some cytokine genes ( IL 1beta IL 1RN , TNF alpha , IFN gamma ) have been correlated with H . pylori associated gastric adenocarcinoma or peptic ulcer , possibly because they influence the amount of cytokine production in response to H . pylori infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The data presented in this work demonstrate that inactivated PPVO has strong effects on cytokine secretion by human immune cells , including the upregulation of inflammatory and Th 1 related cytokines ( IFN gamma , tumor necrosis factor alpha [ TNF alpha ] , interleukin 6 [ IL 6 ] , IL 8 , IL 12 , and IL 18 ) as well as anti inflammatory and Th 2 related cytokines ( IL 4 , IL 10 , and IL 1 receptor antagonist [ IL 1ra ] ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of IL 2 , IFN gamma , IL 4 , IL 10 , and TNF alpha mRNA was determined by a quantitative real time PCR assay . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pretreatment with the peptide LALF ( 32 51 ) differentially regulates TNF alpha , IFN gamma , IL 12p40 , IL 2 and IL 10 mRNA levels in lung and liver of peptide treated mice , and limits the systemic inflammatory response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , the UV inactivated virion induced regional lymph node T cell proliferation and significant levels of cytokine production ( IL 2 , IL 4 , IL 5 , IFN gamma and TNF alpha ) upon restimulation with inactivated SARS CoV virion in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PCB 153 reduced the levels of IL 10 , IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , HIV replication induced by TNF alpha and phorbol ester were not inhibited , and in some cases augmented , by IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma , TNF alpha , IL 10 and IL 12 were up regulated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , cytokines induced by SCG were screened and found to be IFN gamma , tumor necrosis factor alpha ( TNF alpha ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and interleukin 12 ( IL 12p70 ) . ^^^ The addition of recombinant murine GM CSF ( rMuGM CSF ) to spleen cell cultures from various strains of mice synergistically enhanced IFN gamma , TNF alpha and IL 12p70 in the presence of SCG . ^^^ In contrast , neutralizing GM CSF using anti GM CSF monoclonal antibody ( mAb ) significantly inhibited IFN gamma , TNF alpha , and IL 12p70 elicited by SCG . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Also consistent with T cell activation , fratricide induced the transcription and translation of the pro inflammatory cytokines TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Fractalkine / CX3CL1 production by human airway smooth muscle cells : induction by IFN gamma and TNF alpha and regulation by TGF beta and corticosteroids . ^^^ We investigated FKN expression by human ASMC in response to the proinflammatory cytokines IL 1beta , TNF alpha , and IFN gamma , the T helper 2 type cytokines IL 4 , IL 10 , and IL 13 , and the fibrogenic cytokine transforming growth factor ( TGF ) beta . ^^^ Combined stimulation with IFN gamma and TNF alpha induced FKN mRNA and protein expression in a time and concentration dependent manner . ^^^ IFN gamma and TNF alpha induced JNK phosphorylation remained unaltered in the presence of TGF beta but was inhibited by dexamethasone , indicating that JNK is not involved in TGF beta or dexamethasone mediated regulation of FKN production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Toxoplasma can infect and replicate in almost all nucleated cells , and the most important cytokine regulating the growth in humans is IFN gamma ; however , the role of TNF alpha has to date been largely described to be synergistic . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Further comparison of this stimulation with a panel of cytokines ( IL 1alpha , 1beta , 2 13 , and 15 18 ; IFN gamma ; GM CSF ; and TNF alpha ) demonstrated that IL 17 was the most potent cytokine to induce hBD 2 message ( > 75 fold ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Following treatment with either interleukin 1beta , tumor necrosis factor alpha ( TNF alpha ) or gamma interferon ( IFN gamma ) , expression of mCD14 / TLR4 and MyD 88 was determined by flow cytometry and western blotting , respectively . ^^^ RESULTS : IFN gamma stimulated expression of mCD 14 , whereas 1beta and TNF alpha did not . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PBMC from untreated patients in the acute phase , secreted spontaneously IFN gamma , TNF alpha and IL 10 . ^^^ IFN gamma was contributed by T cells , TNF alpha and IL 10 primarily by monocytes and to a lesser extent by T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , IL 17 plus Th 1 or Th 2 cytokine had a modest stimulatory effect on TNF alpha induced G CSF production , whereas IL 17 plus IFN gamma had an inhibitory effect on IL 1beta induced release . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pretreatment of monocytes with IFN gamma , either alone or in combination with TNF alpha , GM CSF , or PPD , significantly enhanced TNF alpha and IL 12 production by deactivated monocytes . ^^^ Treatment of monocytes following coculture with IFN gamma , TNF alpha , GM CSF , PPD or IFN gamma in combination with these agents reversed the depressed TNF alpha release , whereas IL 12 production was enhanced by IFN gamma alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using flow cytometry , AECA were detected against both unstimulated and cytokine stimulated [ tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) ] endothelial cells . ^^^ RESULTS : Compared with controls ( 1 ) Wegener ' s granulomatosis is significantly associated with noncytotoxic AECA that selectively bind surface antigens on unstimulated nasal , kidney , and lung endothelial cells ; ( 2 ) binding of Wegener ' s granulomatosis AECA to kidney and nasal endothelial cells in particular was lost upon treatment with IFN gamma and TNF alpha ; ( 3 ) the two cytokines per se were cytotoxic ( 30 % ) to nasal and lung endothelial cells and lysis was further increased ( 60 % ) by addition of systemic vasculitis serum ; and ( 4 ) Wegener ' s granulomatosis serum caused agglutination of cytokine stimulated nasal endothelial cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon alpha ( IFN alpha ) is a potent suppressor of hepatitis B virus ( HBV ) replication in the HBV transgenic mouse , depleting virus replication intermediates from infected hepatocytes via pathways mediated by interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^ Before their use in vivo , expression of IFN gamma and TNF alpha from the adenovirus vectors was evaluated in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present investigation it is observed that 10microg / ml of rF 1 antigen activated murine peritoneal macrophages in vitro . rF 1 induced the production of TNF alpha , IL 1 , IL 6 , and NO . rF 1 treatment also induced increased transcription of IFN gamma and its related chemokines KC , IP 10 , MIP 1alpha , MIP 1beta , MCP 1 , RANTES in macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have previously shown that T cells can be activated through cell surface C1q receptors , resulting in secretion of interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNFalpha ) , further demonstrating the intimate linkage between innate and adaptive immunity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Administration of PBN protected transplant recipients from mortality by significantly attenuating gut toxicity , but did not effect a reduction in the levels of proinflammatory cytokines ( IL 12 , IFN gamma , tumor necrosis factor alpha [ TNF alpha ] , or nitrate / nitrite ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mitochondrial catalase overexpression also preferentially protected against the toxicity of interleukin 1beta ( IL 1beta ) and a proinflammatory cytokine mixture ( IL 1beta , tumor necrosis factor alpha [ TNF alpha ] , and gamma interferon [ IFN gamma ] ) that is more toxic than IL 1beta alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In order to determine whether loss of SHP 1 creates an aberrant cytokine environment driving the abnormal activation of me / me microglia , the expression of interleukin 4 ( IL 4 ) , interleukin 10 ( IL 10 ) , interleukin 1beta ( IL 1beta ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) was examined by enzyme linked immunosorbent assay ( ELISA ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To explore relevant immunologic mechanisms occurring in KGCR , we have analyzed in surgically removed KG the transcription of the following cytokine and apoptotic molecule genes : interleukin ( IL ) 2 , IL 3 , IL 4 , IL 5 , IL 6 , IL 10 , tumor necrosis factor ( TNF ) alpha , IFN gamma , FAS , and FAS L . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
T cells producing IFN gamma and TNF alpha have been implicated in hematopoietic destruction in AA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study , we have used the microsphere based multiplex method to simultaneously quantitate and compare six analytes , encompassing a representation of the Th1 / Th2 cytokine panel ( interleukin ( IL ) 2 , IL 4 , IL 5 , interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and IL 10 ) , in both serum and culture supernatants from peripheral blood mononuclear cells ( PBMCs ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Administration of NCX 1000 , in a therapeutic manner , that is , 2 h after acetaminophen ( APAP ) intoxication reduced mortality , improved liver histopathology and prevented liver IFN gamma , TNF alpha , Fas / Fas ligand and inducible nitric oxide synthase ( iNOS ) mRNA accumulation caused by APAP . 3 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Infection of RHE with C . albicans induced a strong expression of the chemokine interleukin 8 ( IL 8 ) and the cytokine granulocyte macrophages colony stimulating factor ( GM CSF ) , and a moderate stimulation of interleukin 1alpha ( IL 1alpha ) , interleukin 1beta ( IL 1beta ) , interleukin 6 ( IL 6 ) , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) by keratinocytes . ^^^ Addition of PMNs enhanced a Th 1 type immune response ( IFN gamma , TNF alpha ) , down regulated the expression of the Th 2 type cytokine interleukin 10 ( IL 10 ) , and was associated with protection against Candida induced tissue damage . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In mice , P . acnes priming resulted in increased liver messenger RNA ( mRNA ) and serum levels of tumor necrosis factor alpha , interleukin 12 , and interferon gamma ( IFN gamma ) by both stock LPS and purified LPS challenges compared with nonprimed controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The previous studies have demonstrated that gamma interferon ( IFN gamma ) but not tumor necrosis factor alpha ( TNF alpha ) plays a critical role in pathogenesis of gastritis induced by H . pylori infection . ^^^ In this study we investigated the induction of gastritis induced by H . felis infection in TNF alpha deficient mice , comparing with IFN gamma deficient mice . ^^^ The scores of gastritis and epithelial changes of TNF alpha deficient mice and IFN gamma deficient mice were significantly lower than that of C57BL / 6 mice . ^^^ Moreover , the degrees of gastritis and epithelial changes of TNF alpha deficient mice were rather low compared with that of IFN gamma deficient mice . ^^^ In spleen cell cultures stimulated with heat killed H . felis , IFN gamma production by TNF alpha deficient mice and TNF alpha production by IFN gamma deficient mice were significantly reduced compared with those in C57BL / 6 mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NK cells not only react to , but also produce IFN gamma , TNF alpha , GM CSF , CCL 3 , CCL 4 , and CCL 5 , enabling them to recruit various immune cells to sites of immune response . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
First , under steady state conditions Ly49+CD8+ T cells are poor cytokine producers ( TNF alpha and IFN gamma ) upon TCR triggering . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The present study showed that human oral epithelial cells expressed PR 3 mRNA after treatment with proinflammatory cytokines such as IL 1alpha , TNF alpha , IFN alpha , IFN beta , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After injection of LPS / 2 amino 2 deoxy D galactose ( D gal ) , CD 137 / mice had reduced serum cytokine levels and substantially impaired liver IFN gamma and TNF alpha mRNA levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Analysis of the cellular immune disturbances previously reported in urinary tract infection / inflammation ( increased serum and / or urinary IL 1alpha , IL 1 receptor antagonist , IL 6 and IL 8 ) , steroid sensitive nephrotic syndrome ( increased IL 2 , IFN gamma , TNF alpha , and decreased or increased IL 4 , depending on the cells studied ) , and atopic dermatitis ( decreased IFN gamma and increased IL 4 production ) , may suggest that similar subclinical chronic cytokine mediated abnormalities produced in the course of latent diseases revealed in our patients , combined with those caused by DTP vaccination stimulus , were responsible for the pathomechanism of these clinical entities . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Acute hepatitis and recovery from woodchuck hepatitis virus ( WHV ) infection involves increased intrahepatic expression of interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) mRNAs . ^^^ Transfection of WHV infected primary hepatocytes ( WPH ) from WHV carriers with an IFN gamma expressing plasmid ( pIFN gamma ) resulted in dose dependent accumulations of MHC 1 , TNF alpha , 2 ' 5 ' OAS , and IDO mRNAs within 96 h . ^^^ Thus , IFN gamma expression in carrier WPH induced several host responses often observed in liver of recovering woodchucks , and impaired a stage of WHV DNA synthesis by a non cytolytic mechanism mediated by TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This response is associated with the upregulation of neuronal nitric oxide synthase ( NOS 1 ) , independent of IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
An infection of mice with Plasmodium chabaudi is characterized by a rapid and marked inflammatory response with a rapid but regulated production of interleukin 12 ( IL 12 ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) . ^^^ IFN gamma and TNF alpha play a role in both protective immunity and the pathology of the infection , and the inflammatory disease may be regulated by IL 10 and transforming growth factor beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Histamine suppressed the expression of IL 18 and tumor necrosis factor alpha in the liver , leading to the reduced plasma levels of cytokines including IL 18 , TNF alpha , IL 12 , IFN gamma , and IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Accordingly , the authors used microsphere based flow cytometric immunoassays and hippocampal organotypic cultures ( HOTCs ) to characterize the magnitude , time course , and diversity of cytokine ( interleukin [ IL ] 1alpha , IL 1beta , IL 2 , IL 4 , IL 6 , IL 10 , granulocyte macrophage colony stimulating factor [ GM CSF ] , interferon gamma [ IFN gamma ] , and tumor necrosis factor alpha [ TNF alpha ] ) response to SD . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cells growing in the medium containing 5 . 5 mM glucose and exposed to IL 1 beta , TNF alpha and IFN gamma induced expression of an inducible isoform of nitric oxide synthase ( NOS 2 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Intravenous injection of the vector dramatically suppressed the cytokine production induced by CpG motif pDNA , including TNF alpha , IL 12 and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Fas expression increased in TECs with cytokine treatment ( IFN gamma , TNF alpha ) while abundant FasL levels were not altered . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Molsidomine prevented an 1 R induced increase in superoxide anion and reduced plasma levels of pro inflammatory cytokines ( TNF alpha , IL 1beta and IFN gamma ) , whereas it enhanced anti inflammatory cytokines ( IL 6 and IL 10 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Oral infection of C57BL / 6 mice with Toxoplasma gondii results in small intestinal Th 1 type immunopathology mediated by local production of IFN gamma , TNF alpha , and NO . ^^^ Therefore , neutralization of IL 18 ( rather than TNF alpha , IL 12 , and IFN gamma ) may be a safe strategy for the treatment of Th 1 associated diseases . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neutralizing IFN gamma completely prevented both vascular dysfunction and changes in NOS expression ; neutralizing TNF reduced IFN gamma production and partially prevented dysfunction . ^^^ These effects required T cell activation by class 2 MHC antigens and costimulators ( principally lymphocyte function associated antigen 3 , or LFA 3 ) on the ECs and were mediated by production of soluble mediators including IFN gamma and TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To clarify the immunological state of patients with pulmonary Mycobacterium avium intracellulare complex ( MAC ) disease , we compared the production of IFN gamma , IL 10 and TNF alpha by peripheral blood mononuclear cells ( PBMC ) between MAC disease patients with different levels of disease activity and healthy volunteers . ^^^ PBMC obtained from 46 patients and 15 healthy control volunteers were incubated with a protein antigen extracted from Mycobacterium intracellulare ( PPD B ) for seven days , and IFN gamma , IL 10 and TNF alpha concentration in the supernatant of the cultures was measured by enzyme linked immunosorbent assay ( ELISA ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When PBMC of a subset of TB patients ( n = 11 ) were tested for secretion of protective Th 1 cytokines [ IFN gamma , tumour necrosis factor ( TNF ) alpha and interleukin ( IL ) 12 ] and the immunosuppressive cytokine IL 10 , the complex CF and CW antigens as well as the recombinant Mtb9 . 8 , Mtb9 . 9 , Mtb 40 and Ag85B induced the secretion of both types of cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We analyzed the antiviral effects induced by interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) in cervix carcinoma cells ( HeLa ) and astrocytoma cells ( 86HG39 ) . ^^^ We found that replication of HSV 2 in HeLa cells and in 86HG39 cells is inhibited after stimulation of the cells by IFN gamma and TNF alpha . ^^^ The antiviral effect of IFN gamma is enhanced in the presence of TNF alpha , while stimulation by TNF alpha alone did not induce antiviral activity . ^^^ We found that IFN gamma induces a strong activation of the tryptophan degrading enzyme indoleamine 2 , 3 dioxygenase ( IDO ) and in addition , that the IFN gamma induced IDO activity was enhanced in the presence of TNF alpha . ^^^ Furthermore , we found that the induction of IDO activity is responsible for the inhibition of herpes simplex virus replication , since the presence of excess amounts of l tryptophan abrogates the antiviral effect induced by IFN gamma and the combination of IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expressions of interferon gamma ( IFN gamma ) and tumor necrosis factor alpha of cardiac allografts were upregulated in response to anti PD L 1 mAb treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma , TNF alpha , TWEAK , and TGF beta induced an intracellular redistribution of HMGB 1 and stimulated secretion by THP 1 cells and human blood monocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Besides its well known influence on rheologic properties of blood , PTX has also been found to decrease secretion of some cytokines such as IL 12 , TNF and IFN gamma and thus it could exert immunomodulatory activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The differences in parasite control were associated with a decreased production of IFN gamma and TNF by lymph node cells from L . amazonensis infected mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Because MPO participates in the eradication of Mycobacterium tuberculosis in the in vitro model and the extracellular enzyme may activate cells to cytokine synthesis , we investigated the changes in the enzyme concentration in serum of patients with active pulmonary tuberculosis ( TB ) and correlations between MPO and TNF alpha , IFN gamma , and IL 12 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma decreased the intracellular amount of IL 8 by 60 % in both unstimulated and TNF alpha stimulated cells , but only when secretion was blocked using monensin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the inflammatory component of glial responses to injury by determining mRNA expression of cytokines ( TNF alpha , IL 6 , IL 1beta and IFN gamma ) , chemokines ( MCP 1 and RANTES ) and iNOS in thalamus at day 0 , 1 , 3 and 7 after visual cortex ablation in mice aged 24 ( old ) and 4 months ( young ) . ^^^ Old mice demonstrated higher basal expression of TNF alpha , IL 6 , IL 1beta , MCP 1 , RANTES and iNOS than young mice , while basal IFN gamma expression was lower in the brains of older mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The contributions of tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) to the induction of gastric inflammation and to the protection from H . pylori infection were investigated using TNF alpha geneknockout ( TNF alpha ( / ) ) mice and IFN gamma gene knockout ( IFN gamma ( / ) ) mice . ^^^ The number of H . pylori colonized in the stomach of IFN gamma ( / ) and TNF alpha ( / ) mice was higher than that of wild type mice . ^^^ These findings suggest that TNF alpha and IFN gamma may play a protective role in H . pylori infection . ^^^ Furthermore , we examined the contribution of TNF alpha and IFN gamma to gastric inflammation . ^^^ The CPY 2052 infected TNF alpha ( / ) mice showed a moderate infiltration of mononuclear cells in the lamina propria and erosions in the gastric epithelium as did wild type mice , whereas the CPY 2052 infected IFN gamma ( / ) mice showed no inflammatory findings even 6 months after infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Evaluation of proinflammatory cytokine ( TNF alpha , IL 1beta , IL 6 , IFN gamma ) concentrations in serum and cerebrospinal fluid of patients with neuroborreliosis ] . ^^^ The purpose of this work was to evaluate the concentration of proinflammatory cytokines : IL 1beta , IL 6 , TNF alpha , IFN gamma in serum and cerebrospinal fluid of patients with neuroborreliosis . ^^^ RESULTS : The concentration of measured cytokines IL 1beta , IL 6 , TNF alpha and IFN gamma in serum and cerebrospinal fluid was significantly higher before therapy . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunologic studies showed a significant increase in CTL and NK cytolytic activity ( P < 0 . 05 0 . 001 ) and an up regulated secretion of IFN gamma and TNF alpha ( P < 0 . 01 0 . 001 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OBJECTIVE : In order to explore the role of toll like receptors 2 ( TLR 2 ) in initiating inflammatory response , the expression of TLR 2 of the liver and IL 18 , TNF alpha and IFN gamma of plasma in fulminant hepatic failure was analysed . ^^^ To evaluate the hepatic injury , serum transaminase ( ALT and AST ) and plasma IL 18 , TNF alpha and IFN gamma were determined and the mortality was observed at various time points following the intraperitoneal injection . ^^^ RESULTS : After 4 hours of intraperitoneal injection of D Gal / LPS , the serum transaminase and plasma IL 18 , TNF alpha and IFN gamma levels were elevated . ^^^ The expression of TLR 2 protein was similar to that of the TLR 2 mRNA , and the expression of TLR 2 mRNA was positively correlated with the concentration of plasma IL 18 , TNF alpha and IFN gamma ( r=0 . 36 , P=0 . 02 ; r = 0 . 48 , P 0 . 003 ; r = 0 . 72 , P < 0 . 001 ) at different time points . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Additionally , when Th 1 or Th 2 cytokine was combined with IL 1beta or TNF alpha , both Th 1 and Th 2 cytokines had a modest stimulatory effect on TNF alpha induced GM CSF production , whereas IL 4 and IFN gamma profoundly attenuated IL 1beta induced secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After 1 day in proliferation medium , cells were treated with interferon gamma [ corrected ] ( IFN gamma ; 10 U / ml ) and tumour necrosis factor alpha [ corrected ] ( TNF alpha ; 10 ng / ml ) for 48 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Raised TNFR 2 expression and susceptibility to TNF induced apoptosis was not a general effect of proliferation as IL 1beta and IFN gamma both proliferated TF 1 cells with no effect on TNFR expression or apoptosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We used sections from the synovial membrane of 4 ReA and 4 RA patients which were double stained with immunofluorescence antibodies against cell surface markers for T cells ( CD 3 ) , macrophages ( CD 68 ) and B cells ( CD 20 ) in combination with antibodies against intracellular cytokines TNF alpha , IFN gamma , TGF beta , IL 4 and IL 10 , and quantified these using a fluorescence microscope . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The lung cells from the ginseng treated group produced more interferon gamma ( IFN gamma ) ( P < 0 . 04 ) and tumor necrosis factor alpha ( TNF alpha ) ( P < 0 . 03 ) but less interleukin 4 ( IL 4 ) ( P < 0 . 02 ) with a higher ratio of IFN gamma / IL 4 ( P < 0 . 004 ) after 6 and / or 24 h of incubation with specific and non specific antigens as compared to the control group . ^^^ The ginseng treated splenocytes produced more TNF alpha ( P < 0 . 03 ) and IFN gamma ( P0 . 05 ) than the control spleen cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To identify the novel mechanism by which nitric oxide ( NO ) suppresses flavin containing monooxygenase ( FMO ) activity in endotoxemic rat livers , NO overproducing conditions were induced in primary cultured rat hepatocytes by treatment with a mixture ( LCM ) of lipopolysaccharide and proinflammatory cytokines ( IL 1beta , TNF alpha , and IFN gamma ) , or by the addition of a pure NO donor , spermine NONOate . mRNA levels of the major hepatic form , FMO 1 , decreased via a cGMP independent destabilizing effect of NO rather than by decreased transcription . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum levels of interleukin 4 ( IL 4 ) , IL 6 , IL 10 , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) were determined using enzyme linked immunosorbent assay . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , the ischemic brain damage in IFN gamma transgenic mice was unaffected by recombinant soluble TNF receptor 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of cultured rat pancreatic islets of Langerhans with the combined cytokines interleukin 1beta ( IL 1beta ) , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) leads to DNA damage including strand breakage . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The results showed TNF alpha , IL 1beta could reach the peak with 0 . 06mM nicotine treated for 24 and 12 h on Ana 1 , respectively , but IL 8 and IFN gamma had no significant alter . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokine profile ( interleukin [ IL ] 4 , 5 , 10 and 2 , interferon [ IFN gamma ] , and tumor necrosis factor [ TNF alpha ] ) was determined before the start of treatment and at the end of follow up ( 4 5 months ) . ^^^ Levels of IL 4 , 5 and 10 ( Th 2 profile ) decreased while those of IL 2 , IFN gamma , and TNF alpha ( Th 1 profile ) decreased . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we have investigated the role of endogenous IFN gamma on the antitumor activity of NGR TNF in combination with doxorubicin . ^^^ Synergism between NGR TNF and doxorubicin was observed in immunocompetent mice but not in nude or IFN gamma knockout mice . ^^^ Preadministration of a neutralizing anti IFN gamma antibody to immunocompetent mice inhibited the NGR TNF / doxorubicin synergism , whereas administration of IFN gamma to nude and to IFN gamma knockout mice restored the synergistic activity . ^^^ Administration of NGR TNF in combination with IFN gamma to nude mice , but not of NGR TNF alone , doubled the penetration of doxorubicin in TS / A tumors . ^^^ These findings point to a crucial role for locally produced IFN gamma in tumor vascular targeting with NGR TNF and doxorubicin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
WKE ( 1 mg / ml ) significantly increased interferon ( IFN ) gamma and TNF alpha production compared with the media control ( about 2 . 2 fold for IFN gamma , about 1 . 7 fold for TNF alpha , p < 0 . 05 ) after 24 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytomix ( tumor necrosis factor alpha , IL 1beta , and IFN gamma ) treatment showed that the activity of matrix metalloproteinase 2 ( MMP 2 ) was increased in lung fibroblasts from EGR 1 control ( + / + ) mice but not detected in that of EGR 1 null ( / ) mice , whereas MMP 9 was regulated by EGR 1 in a reverse manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , pretreatment with CpG ODN enhanced the production of TNF alpha , and type 1 cytokines , including IL 12 , IFN gamma , and the IFN gamma dependent ELR CXC chemokines IFN gamma inducible protein 10 and monokine induced by IFN gamma in response to Klebsiella challenge , compared with control mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A fraction ( F 3 ) has been shown to activate the expression of IL 1 , IL 6 , IL 12 , IFN gamma , TNF alpha , GM CSF , G CSF , and M CSF , and from this three subfractions have been prepared where F3G1 activates IL 1 , IL 12 , TNF alpha , and G CSF , F3G2 activates all the cytokines as F 3 does , and F3G3 activates only IL 1 and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Administration of estrogen exposed DC prevented the expansion of CD4+ T cells and increased proportions of regulatory T cells producing IL 10 and CD4+CD28 suppressor T cells , accompanied with increased IL 10 and IFN gamma , and reduced TNF alpha production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Despite reduced NK cell numbers in subjects with ongoing viral replication , these cells were significantly more active in secreting both IFN gamma and TNF alpha than NK cells from aviremic subjects or HIV 1 negative controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Intrarenal transcripts encoding IFN gamma , TNF alpha , IL 12 , and IL 10 , which have been linked to nephritis , were all markedly reduced . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines such as interleukin 1 ( IL 1 ) , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) mediate beta cell dysfunction and islet degeneration , in part , through the induction of the inducible isoform of nitric oxide synthase and the production of nitric oxide by beta cells . ^^^ In this study , we have shown that treatment of rat islets with IL 1beta or human islets with a cytokine mixture containing IL 1beta + IFN gamma + / TNF alpha stimulates COX 2 expression and PGE ( 2 ) formation in a time dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Production of cytokines ( IFN gamma , TNF alpha , IL 2 , IL 10 , TGF beta , and IL 4 ) was determined by intracellular labeling . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here , we provide novel evidence that MMP 12 is expressed and secreted by normal human bronchial epithelial cell cultures ( NHBECs ) and reveal the regulation of MMP 12 gene expression by tumor necrosis factor alpha ( TNF alpha ) , epidermal growth factor ( EGF ) , and interferon gamma ( IFN gamma ) . ^^^ Cultures stimulated independently with EGF or IFN gamma failed to alter MMP 12 mRNA abundance , while TNF alpha , TNF alpha+EGF , or TNF alpha+IFN gamma elicited relatively early ( 6 h ) peak increases in MMP 12 mRNA levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The plasma levels of cytokines ( IL 1beta , IL 6 , TNF alpha , and IFN gamma ) and hypoglycemia induced by LPS were also reduced by urgosedin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MUC 1 expression was evaluated in normal prostate epithelial cells ( PrEC ) , and prostate cancer cell lines in response to dihydrotestosterone ( DHT ) , interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) treatment . ^^^ Furthermore , IFN gamma and TNF alpha strongly induce MUC 1 expression in both normal prostate epithelia and certain prostate tumor cell lines and may exacerbate pathologies associated with MUC 1 positive prostate cancers . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS and RESULTS : Endothelial cells were cultured in a transwell chamber with a membrane pore size of 0 . 45 , 3 . 0 , and 8 . 0 microm and stimulated with TNF alpha , IL 1beta , or IFN gamma from the apical or basolateral side for 16 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 18 , IL 1 beta , and TNF alpha did not induce M CSF , GM CSF , IFN gamma , or OPG production in PHA prestimulated T cells or RA synovial T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation with tumor necrosis factor ( TNF ) and interferon gamma ( IFN gamma ) induced high and low expression of membrane bound FKN on HUVEC and BMVEC , respectively , together with expression of VCAM 1 and intercellular adhesion molecule 1 ( ICAM ) 1 . ^^^ Both CD 16 and CD16+ monocyte subsets adhered to TNF / IFN gamma stimulated HUVEC with higher frequency than to unstimulated HUVEC . ^^^ Monocyte chemoattractant protein 1 ( MCP 1 ) triggered efficient TEM of CD 16 monocytes across TNF / IFN gamma stimulated HUVEC , whereas soluble FKN failed to induce TEM of CD16+ monocytes across stimulated HUVEC . ^^^ These results demonstrate that stimulation with TNF and IFN gamma triggers expression of membrane bound FKN on both HUVEC and BMVEC , but prevents TEM of CD16+ monocytes in response to soluble FKN . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pro inflammatory genes ( tumor necrosis factor alpha [ TNF alpha ] , interferon gamma [ IFN gamma ] , and interleukin 8 [ IL 8 ] ) were expressed at high levels in the psoriatic models , but were only minimally expressed in the normal models . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , interferon gamma ( IFN gamma ) production by NK cells and T cells was increased and stimulated tumor necrosis factor alpha ( TNF alpha ) production by macrophages in the Ad5IkappaB infected liver . ^^^ Moreover , the liver injury was completely suppressed by the administration of anti IFN gamma and anti TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
LIGHT is a member of tumor necrosis factor ( TNF ) superfamily , and previous studies have indicated that in the presence of interferon gamma ( IFN gamma ) , LIGHT through LTbetaR signaling can induce cell death with features unlike classic apoptosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of graded concentrations of Pt , Pd , and Rh salts on spontaneous and PHA stimulated peripheral blood mononuclear cell ( PBMC ) proliferation and IFN gamma , TNF alpha , and IL 5 release were the focus of this study . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Thus , Gl A+ and AG EB+ ENC produce IL 1beta , IL 2 , IL 4 , IL 6 , IFN gamma , TGF beta 1 and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vivo apoptosis of diabetogenic T cells in NOD mice by IFN gamma / TNF alpha . ^^^ BCG immunization sequentially induced the production of TNF alpha , IFN gamma and IL 4 by splenocytes , increased the expression of Fas ( high ) ( Apo 1 / CD95 ) , Fas ligand ( FasL , CD95L ) and TNF receptor ( TNFR ) on T cells leading to T cell apoptosis . ^^^ The primary role of IFN gamma and TNF alpha in BCG immunotherapy was demonstrated by ( 1 ) reversing the immune regulatory effect of BCG by in vivo treatment with neutralizing anti cytokine antibodies , ( 2 ) inducing effect similar to BCG by treatment with these cytokines . ^^^ Our results suggest that BCG down regulates destructive autoimmunity by TNF alpha / IFN gamma induced apoptosis of diabetogenic T cells through both Fas and TNF pathways . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
STAT 1 phosphorylation was also observed upon treatment with IFN gamma but not upon EGF , TNF alpha or IL 6 , and inhibited by the JAK 2 inhibitor AG 490 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
No effect of testosterone was found on IL 2 and IFN gamma producing lymphocytes and TNF alpha producing monocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) , soluble tumor necrosis factor alpha ( TNF alpha ) , soluble Fas ligand ( sFas L ) were present in much higher concentration in the blister fluids of 13 toxic epidermal necrolysis ( TEN ) patients than in control fluids from burns . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mice treated early with anti CD 41 mAb exhibited markedly altered cytokine production on day 4 of P berghei infection ( increased interleukin 10 [ IL 10 ] , IL 1alpha , IL 6 , interferon gamma [ IFN gamma ] , and tumor necrosis factor alpha [ TNF alpha ] ; decreased IL 2 ) but no decline in coagulation factors compared with rat immunoglobulin G ( IgG ) treated controls , indicating that platelets regulate the levels of pathogenic cytokines . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Specifically , the hMSCs caused mature DCs type 1 ( DC 1 ) to decrease tumor necrosis factor alpha ( TNF alpha ) secretion and mature DC 2 to increase interleukin 10 ( IL 10 ) secretion ; hMSCs caused T ( H ) 1 cells to decrease interferon gamma ( IFN gamma ) and caused the T ( H ) 2 cells to increase secretion of IL 4 ; hMSCs caused an increase in the proportion of regulatory T cells ( T ( Regs ) ) present ; and hMSCs decreased secretion of IFN gamma from the NK cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we report that upon specific activation by the physiological ligand HLA C , or Ab cross linking , CD160+ peripheral blood NK cells produce IFN gamma , TNF alpha , and IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We used 12 color flow cytometry to characterize Ag specific T cells elicited by vaccines or natural infection to determine lineage and differentiation status as well as the capacity to produce four cytokines ( IFN gamma , TNF alpha , IL 2 , and IL 4 ) and a chemokine ( MIP1beta ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In THP 1 cells , inhibition of the expression of M CSF , G CSF , stem cell factor , IFN gamma , TNF alpha , TGF beta 1 , lymphotoxin beta 1 , MIP 1alpha , MIP 1beta , and IL 8 was observed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The variables considered included : 1 ) relation with the activity of disease , 2 ) extra articular manifestations ( EAM ) , 3 ) synthesis of cytokines ( IL 4 , IL 10 , IL 12 , TNF alpha , and IFN gamma ) and IgM and IgA rheumatoid factor ( RF ) , and 4 ) the association with HLA DRB 1 polymorphism . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
INTRODUCTION : Mycobacterium tuberculosis is a facultative intracellular pathogen that developes specific T cell response expressed by the production of IL 12 , IFN gamma , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These CD4+ / CD8alpha+ / TCR ( POS ) T lymphocytes produce IFN gamma , TNFalpha and IL 2 when specifically stimulated via the introduced TCR with immobilized HLA A1 / MAGE A 1 complexes or HLA A1+ / MAGE A1+ melanoma cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To explore the difference of negative regulatory factors among T lymphocyte subsets in bone marrow ( BM ) of myelodysplastic syndromes ( MDS ) and their relations to apoptotic gene Fas , different lymphocyte subsets in BM were categorized by monoclonal antibodies with 3 color fluorescence using flow cytometry , and the intracellular cytokines such as tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) were determined following marrow cells culture . ^^^ The results showed that TNF alpha , IFN gamma levels in BM of MDS both increased , the former produced by cells CD4+CD45RO+ , CD8+CD45RO+ , the latter by cells CD4+CD45RO+ , CD8+CD45RO+ , CD8+CD45RA+ , in which the cells CD8+CD45RO+ were dominant . ^^^ Fas mRNA expression had relationship with IFN gamma produced by T cells but not with TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mice immunized up to 6 months previously with S . enterica serovar Typhimurium had bacterium specific CD4+ T cells that were capable of producing IFN gamma or tumor necrosis factor alpha ( TNF alpha ) at each site analyzed . ^^^ Similar findings were observed for CD8+ T cells that were capable of producing IFN gamma , while a much lower frequency and more restricted distribution were associated with TNF alpha producing CD8+ T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Multiple cytokines were also expressed , including IL 1alpha , IL 1beta , IL 2 , IL 4 , IL 6 , IL 10 , IL 12 , IL 18 , TNF alpha , and IFN gamma in kidney allografts on days 3 , 5 , and 7 after grafting , as measured by a ribonuclease protection assay . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
ZK 192 also decreased mucosal mRNA and protein levels of T helper 1 effector cytokines : tumor necrosis factor alpha , IL 2 , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
On day 3 after initiation of the cultures , IL 2 , IFN gamma , IL 4 , IL 10 , TNF alpha content were determined in supernatants of PBMC from the different individuals . ^^^ No significant changes were observed between 1 10 microM concentrations in the production of TNF alpha , IFN gamma and IL 10 by both PHA and TT stimulated PBMC . ^^^ Ribavirin inhibited TNF alpha , IFN gamma , and IL 10 in both PHA and TT stimulated PBMC at 100 microM ( p < 0 . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum levels of cytokines ( IL 4 , IL 5 , IFN gamma , TNF alpha ) , cytokine receptors ( TNFR 1 and 2 ) and one monokine ( neopterin ) were estimated in seven leprosy patients to establish disease associated markers for reversal reactions ( RR ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma , TNF alpha , and IL 10 positive cells were consistently predominant , whereas IL 4 was scarce in the valves . ^^^ The predominant IFN gamma and TNF alpha expression in the heart suggests that Th 1 type cytokines could mediate RHD . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PURPOSE : To correlate tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) synthesis with histopathologic disease and virus replication within murine cytomegalovirus ( MCMV ) infected eyes during progression of murine acquired immunodeficiency syndrome ( MAIDS ) . ^^^ MCMV inoculated eyes from all mice were subjected to histopathologic analysis , quantitative plaque assay , or cytometric bead array analysis for quantification of TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Whole blood samples from 47 subjects [ 24 patients with SLE , 11 with rheumatoid arthritis ( RA ) as a disease control , and 12 healthy negative controls ] were stimulated with VZV antigen , stained for surface CD 4 and CD 8 and intracellularly stained for the cytokines interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 4 ( IL 4 ) , and IL 10 , followed by flow cytometry analyses . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Incubation of human hepatocytes with single cytokines ( IFN gamma , IL 1beta , TNF alpha ) or LPS , as well as a combination of all four stimuli ( CM , cytomix ) caused a time dependent HO 1 mRNA expression over 12h and a decline by 24 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Exposure of differentiated IMR 32 cholinergic nerve cells to IL 1beta , TNF alpha , and IFN gamma , or culture in serum deprived medium , induced neuronal apoptosis , as detected by annexin 5 staining , caspase 3 activation , and DNA laddering . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our results indicate that hTERT expressing HIV specific CD 8 ( + ) lymphocytes show both an enhanced and sustained capacity to inhibit HIV 1 replication in in vitro coculture experiments , as well as prolonged ability to produce IFN gamma and TNF alpha in response to stimulation with HIV 1 derived peptides , as compared with vector transduced controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Exposure of HBMEC to various cytokines including TNF alpha , IL 1beta , interferon gamma ( IFN gamma ) , or lipopolysaccharide ( LPS ) decreased transendothelial electrical resistance ( TEER ) mainly by increasing the permeability of the tight junctions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increased mRNA expression for IFN gamma and TNF alpha was registered correlating with the peak of clinical signs of EAN , and high levels of mRNA expression for IL 4 , IL 10 , and TGF beta were associated with EAN recovery . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
SHIV VLPs efficiently stimulated DCs to release IL 12 , IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Production of tumor necrosis factor alpha , interferon gamma ( IFN gamma ) , and prostaglandin E ( 2 ) ( PGE ( 2 ) ) was measured by enzyme linked immunosorbent assays after stimulation with mitogen and endotoxin ( lipopolysaccharide ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
BACKGROUND : Inflammatory bowel disease ( IBD ) is associated with altered apoptosis and increased levels of Th 1 cytokines ( IL 12 , TNF alpha , and IFN gamma ) . ^^^ MATERIALS AND METHODS : Lymphocytes with Stat 6 ( null phenotype ) ( n = 5 ) or wild type ( n = 5 ) status were cultured with and without the addition of exogenous cytokines or neutralizing antibodies ( IL 12 , TNF alpha , and IFN gamma ) . ^^^ Increased levels of IL 12 and IFN gamma were found in the Stat 6 ( null phenotype ) cell lines ; however , the apoptosis observed is not the consequence of increased IL 12 , IFN gamma , or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effect was associated with a strong swelling , a rapid influx of polymorphonuclear ( PMN ) cells , and an elevated secretion of TNF alpha and IFN gamma by lymph node cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of Th 1 ( IL 2 , IFN gamma , TNF alpha ) and Th 2 ( IL 4 ) mRNAs was examined by quantitative real time RT PCR in the ear samples . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Secretion of the Th 1 cytokines IL 2 and IFN gamma was reduced by 50 % or more with 0 . 45 0 . 6 SGP / mL , as was secretion of the pro inflammatory cytokines GM CSF and TNF alpha , and the Th 2 cytokine IL 5 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
No differences were shown for P . falciparum specific IFN gamma and IL 10 expression within CD3+ cells and parasite non specific expression of IL 2 , IL 4 , IL 6 , IL 10 , IL 13 , TNF alpha , and IFN gamma within the CD4+ , CD8+ , TCRgamma \ delta+ CD3+ and CD94+ CD 3 cell populations , indicating that immunological determinants regulating the susceptibility to malaria in age matched children are parasite specific . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study we investigated the relationship between the total number of DC ( tDC ) , and their plasmacytoid ( pDC ) and myeloid ( mDC ) subpopulations , with serum concentrations of interferons ( IFN alpha and IFN gamma ) and selected cytokines ( TNF alpha , IL 4 , IL 6 ) , in patients with systemic lupus erythematosus ( SLE ) and healthy persons . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro production of proinflammatory cytokines such as TNF alpha , IFN gamma and IL 12p70 , by antigen stimulated splenocytes from mice intravenously infected with the low virulence C . albicans PCA 2 strain , could not be detected in MyD 88 \ mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum sIL 2R , TNF alpha and IFN gamma in alveolar echinococcosis . ^^^ AIM : to approach the relationship between alveolar echinococcosis ( AE ) pathology and level of sIL 2R , TNF alpha and IFN gamma in sera and the significance of cytokines in development of AE . ^^^ METHODS : After 23 patients with AE were confirmed by ELISA and ultrasound , their sera were collected and the concentrations of sIL 2R , TNF alpha and IFN gamma were detected by double antibody sandwich . ^^^ Average of concentrations of sIL 2R , TNF alpha and IFN gamma in homologous group was statistically analyzed by both ANOV and Newman Keuls , respectively . ^^^ Judged by ANOV and Newman Keuls , the mean concentrations of sIL 2R , TNF alpha and IFN gamma had a significant difference among groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Infection with either bacteria similarly resulted in increased milk levels of IL 1 beta , IL 8 , IL 10 , IL 12 , IFN gamma , TNF alpha , sCD 14 , LBP , and the complement component , C5a . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
On the other hand interferon gamma ( IFN gamma ) and tumor necrosis factor ( TNF ) increase Fas expression on erythroid cells and enhance their apoptosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Cultured keratinocytes constitutively expressed the H 1 receptor mRNA and protein , which was not influenced by IFN gamma , TNF alpha , or IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here we have compared the ability of TLR stimuli and the adaptive immune cytokines interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) to induce a variety of immunologic functions of astrocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha ( tumour necrosis factor alpha ) , IL ( interleukin ) 1 , IL 6 and IFN gamma ( interferon gamma ) ] and growth factors that promote the development of functional and structural vascular changes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , the occupancy of the CD94 / NKG2 A receptor expressed on these superantigen stimulated CD4+ T lymphocytes abrogates TNF alpha and IFN gamma production , whereas NKG 2 C enhances production of these cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , HO 1 induction did not affect the production of NO by rat alveolar epithelial type 2 cells that were stimulated with cytomix ( a mixture of TNF alpha , IL 1beta , and IFN gamma ) for 24 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
ONO 4847 also inhibited increases in the mucosal TNF alpha and IFN gamma content after DSS administration . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines including IL 1beta , IL 2 , IL 4 , IL 6 , IL 8 , TNF alpha , and IFN gamma were measured by commercially available ELISA kits in SCD patients ( n = 60 ) ; in steady state ( n = 26 ) and in painful crisis ( n = 34 ) and compared with nonanemic age and sex matched normal Omani controls ( n = 20 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Real time reverse transcription polymerase chain reaction ( RT PCR ) analyses indicated that the cDNA copy numbers of IL 1beta , IL 4 , IL 6 , IL 10 , TNF alpha and IFN gamma from the skin of late , early and non lesions increased significantly compared to non Tg mice . ^^^ Interestingly , IL 1beta , IL 3 , IL 4 , IL 5 , IL 6 , IL 10 , IL 13 , TNF alpha , and IFN gamma cDNAs were increased significantly the skin of before onset and / or non lesion mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum samples , PBMC and T cell lines ( TCL ) obtained from 54 patients affected by SSc and 20 healthy donors ( HD ) were examined by ELISA for Interferon gamma ( IFN gamma ) , interleukin ( IL ) 4 , IL 6 , IL 10 , IL 18 , Transforming growth factor ( TGF ) beta 1 , Tumour necrosis factor ( TNF ) alpha , sCD 30 , Macrophage derived chemokine ( MDC ) , Monocyte chemoattractant protein ( MCP ) 1 , Macrophage inflammatory protein ( MIP ) 1alpha and Regulated on activation normal T cell expressed and secreted ( RANTES ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To search for useful laboratory measures of central nervous system ( CNS ) immunity that may provide an accurate prognosis or clues regarding treatment choice , cerebrospinal fluid ( CSF ) samples were obtained from 14 consecutive patients with acute encephalitis during acute as well as convalescent or chronic stages , and then examined for surface antigen expressions by lymphocytes and the presence of antineuronal tissue antibodies as well as the levels of IgG related parameters and proinflammatory cytokines , including IL 2 , IL 6 , IFN gamma , and tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and transforming growth factor beta ( TGF beta ) were also examined . ^^^ The levels of TNF alpha were lower , whereas IFN gamma and TGF beta 1 were higher . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha , IL 1 beta , IFN gamma , IL 4 , IL 10 and CINC ( a rat cytokine induced neutrophil chemoattractant and member of the IL 8 family ) were determined by enzyme immunoassay . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Aim of this investigation was to compare the effects of 10 ( 4 ) M and 10 ( 7 ) M As compounds on spontaneous and PHA stimulated PBMC proliferation and IFN gamma and TNF alpha release . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The levels ( mean + / SD pg / ml ) of Th 1 cytokines ( IFN gamma and TNF alpha ) and Th 2 interleukines ( IL 10 and IL 4 ) were also determined . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Two and one of 4 CR / PR pts had increased IFN gamma and TNF alpha production with no TGF beta 1 responses by their PBMC after 3 treatments , respectively . ^^^ Two out of 6 pts who experienced stable disease after the treatment had high IFN gamma and TNF alpha responses and no TGF beta 1 or IL 4 response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Changes and significance of TNF , sIL 2R , IL 6 , IL 4 and IFN gamma levels in plasma from the patients with hemorrhagic fever with renal syndrome ] . ^^^ AIM : To explore the changes of TNF , sIL 2R , IL 6 , IL 4 and IFN gamma levels in plasma from patients with hemorrhagic fever with renal syndrome ( HFRS ) and analyze the correlation between these cytokine levels and alanine aminotransferase ( ALT ) level in sera from the patients . ^^^ RESULTS : Levels of TNF , IL 6 , IL 4 , IFN gamma and sIL 2R in plasma from the patients were ( 95 . 82+ / 12 . 04 ) , ( 362 . 46+ / 141 . 26 ) , ( 17 . 76+ / 3 . 52 ) , ( 116 . 18+ / 19 . 80 ) ng / L and ( 898 820+ / 127 200 ) U / L , respectively . ^^^ Statistical analysis showed that levels of TNF , sIL 2R , IL 6 and IFN gamma were positively correlated with ALT level , respectively . ^^^ CONCLUSION : The HFRS patients have a significant increase in both cytokines ( TNF , sIL 2R , IL 6 and IFN gamma ) and ALT levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Anti IFN gamma mAb shortened the survival time of infected mice and reduced the recruitment of neutrophils and production of MIP 2 and TNF alpha in the lungs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We then analysed the effects of IFN gamma , TNF alpha , IL 12 , IL 15 , IL 18 , and LPS on CXCL 16 expression in cultured aortic smooth muscle cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
They produce high levels of IFN gamma and TNF alpha , but little or no IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Estrogen ( E ) deficiency leads to an expansion of the pool of tumor necrosis factor ( TNF ) producing T cells through an IFN gamma dependent pathway that results in increased levels of the osteoclastogenic cytokine TNF in the bone marrow . ^^^ This phenotype results from a failure of E to repress IFN gamma production , which , in turn , leads to increased T cell activation and T cell TNF production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There was a dose dependent , suppressive effect of hemozoin on production of gamma interferon ( IFN gamma ) , with less of an effect on tumor necrosis factor alpha ( TNF alpha ) and interleukin 10 , in human immunodeficiency virus seronegative ( HIV ( ) ) women . ^^^ In contrast , IFN gamma and TNF alpha production tended to increase in HIV seropositive women with increasing hemozoin levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
While the addition of IL 2 , anti CD40L and allogeneic cells led to partial increase in the ability of the tetramer+ cells to synthesize IFN gamma , the addition of IL 4 , IL 12 , anti CD 28 or a cocktail of antiTGF beta , TNF alpha and IL 10 failed to augment the IFN gamma response . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There are many molecules , including Fas ligand ( FasL ) and cytokines , such as IL 1 , TNF alpha and IFN gamma that cause release of other cytokine mediators that have potential to damage the beta cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here , we show how three pro inflammatory cytokines ( IL 1beta , TNF alpha , and IFN gamma ) together stimulated the activation , but not the prior expression , of NOS 2 protein via a mechanism involving MEK ERKs protein kinases in astrocytes from adult human cerebral temporal cortex . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this work we describe the effect that thalidomide had on TNF alpha sera levels and on IL 4 and IFN gamma ( IFNgamma ) producing lymphocytes of actinic prurigo ( AP ) patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This review discusses the biology and therapeutic potential of selected immune modulatory cytokines and their inhibitors , including granulocyte colony stimulating factor , granulocyte macrophage colony stimulating factor , IFN gamma , IL 12 and TNF . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
LMP 1 mediated IP 10 expression is independent from IFN gamma , TNF alpha or IL 18 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
ODN induce natural killer cell activity and induce IFN gamma , TNF alpha , and IL 6 from mononuclear cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The following indices were evaluated : fluid requirements for resuscitation , mean arterial pressure ( MAP ) , arterial po 2 , pco 2 , and pH , lung wet to dry weight ratio , lung histology and cytokine ( interleukin [ IL ] 1 alpha , IL beta 1 , tumor necrosis factor beta [ TNF beta ] , IL 3 , IL 4 , IL 5 , IL 6 , IL 10 , TNF alpha , IL 2 , interferon gamma [ IFN gamma ] ) , and mRNA expression in the lung by a ribonuclease protection assay ( RPA ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using intracellular staining and flow cytometry , we assessed the ability of freshly isolated liver T cells from these biopsies to produce IFN gamma , TNF alpha , IL 2 , IL 4 , and IL 10 in response to stimulation with PMA and ionomycin . ^^^ TNF alpha ( 30 . 3 + / 13 % vs . 15 . 5 + / 5 % , P = 0 . 01 ) , IL 2 ( 20 . 2 + / 9 % vs . 10 + / 4 % , P = 0 . 01 ) and IFN gamma ( 22 . 2 + / 11 % vs . 9 . 4 + / 4 % , P = 0 . 008 ) ] , whereas IL 10 , a representative Th 2 type cytokine , was significantly lower ( 7 . 2 + / 4 % vs . 17 + / 7 % , P = 0 . 01 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
On the other hand , production of IFN gamma and TNF alpha was slightly stimulated by the lowest ozone dose either at all times or only after 72 h incubation , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
As result , administration of anti CD 3 antibodies in vivo induced markedly increased blood plasma IL 2 levels ( but not IL 4 , IFN gamma , TNF alpha and IL 6 levels ) in the PKCdelta null mice , when compared to wild type sibling controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The Th 1 [ interferon gamma ( IFN gamma ) , TNF alpha , and IL 12 ] and Th 2 ( IL 4 and IL 10 ) cytokines have opposite effects on human pregnancy . ^^^ Leukemia inhibitory factor ( LIF ) promotes embryo implantation and sustains pregnancy , whereas IFN gamma and TNF alpha are detrimental to pregnancy . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The aim of this study was to relate cerebrospinal fluid ( CSF ) concentrations of IL 6 , IL 8 , IL 10 , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) to magnetic resonance defined white matter injury in preterm infants . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
LTK 63 treatment increased the pathogen specific IgA response in the nasal mucosa and simultaneously decreased inflammatory cytokine production ( IFN gamma and TNF alpha ) after infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This system named `` fluorescent cell chip ' ' ( FCC ) is based on a number of genetically modified cell lines that regulate the expression of a transgene coding for fluorescent protein enhanced green fluorescent protein ( EGFP ) in a similar way as they regulate expression of IL 1beta , IL 2 , IL 4 , IFN gamma , IL 10 , TNF alpha , and beta actin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , the host defence mechanisms evoked by two different immunocompetent murine strains following oral colonization with Candida albicans were assessed . beta Defensin ( mBD 1 , mBD 3 and mBD 4 ) , chemokine ( MIP 2 and KC ) and cytokine ( TNF alpha , IFN gamma , IL 4 , IL 10 , IL 12 and IL 15 ) gene expression in germ free ( gf ) and C . albicans infected ( gastric ) C57BL / 6 and BALB / c mice was contrasted . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 12 induced colitis was accompanied by the local expression of IFN gamma and TNF alpha but not IL 4 mRNA and protein . ^^^ Conversely , IL 4 induced colitis was accompanied by the local expression of IL 4 and TNF alpha but not IFN gamma mRNA and protein . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Some cytokines , such as TNF alpha , IL 1 and IFN gamma , are well known for their promotion of inflammatory responses . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF augments inflammation , TNF and IFN gamma induce coagulation , and IL 1beta induces coagulation and fibrinolysis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Recipients and donors were typed for TNF alpha ( 308G / A ) , TGF beta 1 ( codon 10T / C , codon 25C / G ) , IL 10 ( 1082G / A , 819C / T , 592C / A ) , IL 6 ( 174C / G ) , and IFN gamma ( +874T / A ) polymorphisms using a SSP PCR kit . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma , IL 18 , TGF beta , IL 6 , IP 10 , MCP 1 , MIG , and IL 8 , but not of TNF alpha , IL 2 , IL 4 , IL 10 , IL 13 , or TNFRI , were highly elevated in the acute phase sera of Taiwan SARS patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We studied these Vdelta 1 T lymphocytes and observed that they proliferated in vitro and produced tumor necrosis factor alpha or IFN gamma in response to autologous leukemic B cells but not to normal lymphocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To investigate the intra and extracellular levels of various cytokines and chemokines in CSF in chronic inflammatory demyelinating polyneuropathy ( CIDP ) and vasculitic neuropathy ( VN ) , 16 cytokines , IL 1beta , IL 2 , IL 4 , IL 5 , IL 6 , IL 7 , IL 8 , IL 10 , IL 12 ( p 70 ) , IL 13 , IL 17 , IFN gamma , TNF alpha , G CSF , MCP 1 and MIP 1beta , were measured in CSF supernatant by a multiplexed fluorescent bead based immunoassay and intracellular production of IFN gamma and IL 4 in CSF CD4+ T cells were simultaneously measured by flow cytometry in 14 patients with CIDP , 8 patients with VN and 25 patients with other noninflammatory neurologic diseases ( OND ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A dramatic increase in the MHC class 1 expression level was observed when the cells were treated with IFN gamma , TNF alpha , and IL 1beta , alone or in combination . ^^^ The apparent additive effects of TNF alpha and IL 1beta in combination on the maximum induction of MHC class 1 expression exerted by IFN gamma treatment were not observed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
DC 1 induction depends on NK cell produced IFN gamma and TNF alpha , with a possible involvement of additional factors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In a HaCaT keratinocyte model , we now demonstrate that IFN gamma and to a less extent also TNF alpha and TGF alpha are able to induce K 17 protein expression , in contrast to IL 1alpha , IL 1beta , IL 6 , IL 8 and IL 18 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Duodenal biopsies from untreated , treated celiac patients and controls were used to quantify the expression of MMP 1 , MMP 2 , MMP 3 , MMP 9 , MMP 12 , MMP 14 , their inhibitor TIMP 1 , IFN gamma and TNF alpha by using real time reverse transcription polymerase chain reaction and the gelatin / casein / elastin activities by gel zymography , and to isolate lamina propria mononuclear cells ( LPMCs ) . ^^^ These cells and myofibroblasts isolated from jejunal specimens were incubated in the absence or presence of IFN gamma and TNF alpha . ^^^ In active CD , transcripts of TIMP 1 were higher than in treated and controls ( P < 0 . 005 and P < 0 . 05 , respectively ) , such as those of IFN gamma ( P < 0 . 05 ) , whereas TNF alpha levels were suppressed ( P=0 . 0001 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study , when tuberculous BALB / c mice were treated with EpiBr 3 times / week beginning on day 60 , inhibition of bacterial proliferation and increased expression of TNF alpha , IFN gamma , and iNOS were observed , although decreased expression of IL 4 was also observed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma and TNF alpha production and clonal expansion of specific CD 8 ( + ) T cells from normal and knockout mice are similar . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
DC activation induced by both IPP and PAM stimulated gammadelta T cells was mostly mediated by TNF alpha and IFN gamma secreted by activated lymphocytes . ^^^ In fact , when PAM stimulated DC gammadelta T cell cocultures were separated by a semipermeable membrane or treated with blocking anti CD 86 Abs , induction of CD 25 and CD 69 as well as IFN gamma and TNF alpha secretion by Vdelta 2 cells were strongly reduced . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This decrease corresponded to reduced TNF alpha and IFN gamma levels in the heart , suggesting that macrophage and / or neutrophil populations may be a primary source of TNF alpha and IFN gamma during acute CVB 3 myocarditis . ^^^ However , STAT 4 or IFN gamma deficiency resulted in significantly increased viral replication and significantly reduced TNF alpha and IFN gamma levels in the heart , similar to IL 12 deficiency , indicating that the IL 12 / STAT4 pathway of IFN gamma production is important in limiting CVB 3 replication . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The disease activity index ( DAI ) , histological changes of the colon , myeloperoxidase ( MPO ) activity and production of cytokines ( TNF alpha , IL 4 , IFN gamma ) by the mucosa were evaluated . ^^^ RESULTS : There were obvious changes in the DAI , histology and MPO activity , and the production of interleukin 4 was markedly increased compared with the concentrations of TNF alpha and IFN gamma , which remained normal , in the lesions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Recent studies have demonstrated that influenza virus infection induced the gene expression of a set of pro inflammatory cytokines , such as IL 1beta , IL 6 , TNF alpha , interferon ( IFN ) beta , IFN gamma and granulocyte macrophage colony stimulating factor ( GM CSF ) , and the secretion of unidentified monocyte differentiation inducing factor ( s ) from primary cultured chorion cells undergoing apoptosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Transient neutralization of TNF alpha resulted in transient depression of interleukin 12 ( IL 12 ) , monocyte chemotactic protein 1 ( MCP 1 ) , and gamma interferon ( IFN gamma ) production but permanently impaired long term clearance of the infection from the lungs even after the levels of these cytokines increased and a vigorous inflammatory response developed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , IFN gamma and tumor necrosis factor alpha production in vitro was significantly inhibited by sera from mSEC immunized mice but not by sera from control mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pravastatin and fluvastatin also induced the production of IL 18 , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) in human peripheral blood mononuclear cells ( PBMC ) in contrast to LFA 703 . ^^^ In the presence of higher concentrations ( 5 , 10 , and 100 ng / ml ) of IL 18 , pravastatin , fluvastatin , and LFA 703 similarly inhibited the expression of ICAM 1 and CD 40 as well as the production of IL 12 , TNF alpha , and IFN gamma in PBMC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found that GT ( 0 . 1 approximately 0 . 25 mg / ml ) exerted dose dependent inhibitions on the release of TNF alpha and IL 6 in LPS activated human whole blood and peripheral blood mononuclear cells ( PBMC ) , and IFN gamma in PHA stimulated human whole blood . ^^^ Luteolin , the deglycosylated derivative of one of the major compositions , luteolin 7 glucoside , exerted inhibitory effects on TNF alpha , IL 6 and IFN gamma production in activated human whole blood with estimated IC ( 50 ) s of 42 . 73 microM , 44 . 86 microM and 3 . 34 microM , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the association between the role tumor necrosis factor alpha ( TNF alpha ) , transforming growth factor beta ( TGF beta ) , interferon gamma ( IFN gamma ) , interleukin ( IL ) 10 and IL 6 gene polymorphisms and early graft rejection among liver transplant recipients . ^^^ In conclusion , there was no significant correlation between early graft rejection and cytokine gene polymorphism of TNF alpha , TGF beta , IL 10 , IL 6 , and IFN gamma in liver transplant recipients . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The results obtained from freshly isolated PBMC showed expressions of TNF alpha , IL 2 , IFN gamma , IL 10 and IL 18 mRNA , similar to those from non infected dogs . ^^^ In vitro stimulation of PBMC with soluble leishmanial antigen ( SLA ) promoted the expression of IL 2 , IFN gamma , TNF alpha , IL 18 , IL 4 , IL 6 and IL 10 mRNA , with the two first being specifically induced . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Real time RT PCR has been recognised to be a more accurate and sensitive method of quantifying mRNA transcripts , so in this study TaqMan real time RT PCR assays for the quantification of mRNA encoding IL 2 , IL 4 , IL 5 , IL 6 , IL 10 , IL 12 , IL 18 , IFN gamma , TNF alpha and TGF beta in canine intestinal mucosa were developed . ^^^ IL 18 , TGF beta and TNF alpha were found to be the most abundant transcripts , with IL 10 and IFN gamma present at levels approximately 10 fold less . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A panel of three CpG oligodeoxynucleotides ( ODN ) and three eukaryotic expression vectors currently used in experimental DNA vaccines in pigs ( pcDNA 1 , pcDNA3 . 1 and pCI ) were screened for their immunostimulatory activity on porcine PBMC by evaluating in vitro the lymphocyte proliferative responses and cytokine profiles ( IL 1alpha , IL 2 , IL 4 , IL 6 , IL 10 , IFN gamma , TGF beta , TNF alpha ) . ^^^ Furthermore , CpG ODN A significantly induced IL 6 and TNF alpha together with elevated levels of IFN gamma and IL 2 mRNA expression even though considerable heterogeneity was observed in the response of individual pigs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Concentrations of cytokines ( IL 4 , IFN gamma , IL 6 and TNF alpha ) were measured in plasma and whole blood after PMA / ConA and PMA / ionomycin stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The neutralization of either IL 12 induced IFN gamma or LPS induced TNF alpha improved the survival of middle aged ( 25 week old ) mice . ^^^ Both IFN gamma production after IL 12 priming and TNF alpha production from the liver mononuclear cells after LPS challenge were also prominent in the middle aged mice . ^^^ Although the depletion of NK / NK T cells did not decrease the IFN gamma or TNF alpha production in the Shwartzman reaction of the middle aged mice , an additional depletion of CD8+CD122+ T cells did decrease such production and also improved mouse survival . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We here report that a combination of IL 1beta and IFN gamma or a combination of TNF alpha and IFN gamma very markedly induced HGF production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Death associated protein ( DAP ) kinase plays an important role in IFN gamma , tumor necrosis factor ( TNF ) alpha , or Fas ligand induced apoptosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The TNF superfamily cytokine , lymphotoxin like inducible protein that competes with glycoprotein D for binding herpesvirus entry mediator on T cells ( LIGHT ; TNFSF 14 ) , can augment T cell responses inducing IFN gamma production and can drive pathological gut inflammation when expressed as a transgene in mouse T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These T cells were fully functional in terms of their ability to produce IFN gamma and TNF alpha and to mediate cytolytic activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inflammatory cytokines such as IFN gamma and TNF produced by Ag stimulated CD 4 ( + ) and CD 8 ( + ) T cells are important in defense against microbial infection . ^^^ Previous studies , conducted with BALB / c mice , have suggested that 1 ) CD 8 ( + ) T cells maintain IFN gamma production but transiently produce TNF in the continued presence of Ag and 2 ) lymphocytic choriomeningitis virus specific and in vitro propagated effector CD 8 ( + ) T cells could rapidly cycle IFN gamma production ON / OFF / ON in response to Ag exposure , removal , and re exposure . ^^^ In contrast with CD 8 ( + ) T cells , our results show that Listeria monocytogenes specific CD 4 ( + ) T cells from C57BL / 6 mice rapidly initiate ( ON cycling ) and maintain production of both IFN gamma and TNF in the continued presence of Ag . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Production of this signal , which is not IL 12 , IL 18 , IL 23 , IL 27 , or IFN gamma and is not provided via Th 1 cells , is dependent upon a MyD 88 dependent , TNF receptor associated factor 6 independent signaling pathway in DCs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma , TNF alpha , IL 4 , IL 10 and IL 12 concentrations in the supernatant of peripheral blood mononuclear cell ( PBMC ) cultures and the in vitro proliferation of PBMC were studied in 25 patients with actinomycetoma caused by Nocardia brasiliensis and in 10 healthy controls from endemic zones . ^^^ IFN gamma production was higher in the healthy controls than in the patients , whereas TNF alpha secretion was slightly higher in the patients ' cultures . ^^^ These results suggest that patients with actinomycetoma show normal antigen recognition , but with low IFN gamma production , and higher concentrations of IL 4 , IL 10 and TNF alpha in the patients ' PBMC cultures , indicating that they probably have a Th 2 type of immune response . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The immunomodulatory properties of these four crude extracts and PS 50 fractions were investigated by the measurement of nitric oxide ( NO ) , tumor necrosis factor alpha ( TNF alpha ) , and interleukin 10 ( IL 10 ) production using interferon gamma ( IFN gamma ) activated macrophages and non activated macrophages as control . ^^^ In contrast , IFN gamma activated J 774 macrophages synergized with PS 50 , but not with the three other crude extracts , to produce both NO and TNF alpha , but not IL 10 . ^^^ In addition , F 2 fraction from PS 50 was particularly active in enhancing NO and TNF alpha ( but not IL 10 ) production by IFN gamma activated C3H / Hej macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , when various cytokines / factors were added to such FLS cell lines , we found that IFN gamma or TNF alpha were unique in that they could induce significant increases in BAFF mRNA and protein . ^^^ Even minute amounts of IFN gamma primed FLS for TNF alpha , allowing the latter to stimulate significantly higher levels of BAFF mRNA and protein than could TNF alpha alone . ^^^ Consistent with this , B cells cocultured with IFN gamma and / or TNF alpha treated FLS had a significantly greater viability than B cells cocultured with nontreated FLS . ^^^ The enhanced protection of B cells afforded by IFN gamma / TNF alpha treated FLS was inhibited by the addition of BAFF R : Fc fusion protein . ^^^ We conclude that the proinflammatory cytokines IFN gamma and TNF alpha can induce mesenchymal derived FLS to express functional BAFF in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OprI triggered dendritic cells to make IL 12 and TNF alpha , with subsequent IFN gamma production from T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
LPS induced increases in plasma interleukin 1beta ( IL beta ) , IL 6 , interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) and blood glucose levels were significantly inhibited by eugenosedin A ( 1 mg kg ( 1 ) , 1 . v . ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
LPS stimulation induced more IL 6 , IL 12 , and TNF alpha , and anti CD 3 induced more IFN gamma and IL 4 by peritoneal cells from pristane treated mice vs . control . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The variation of HPRE might affect the function of HPRE and influence the regulative function of IFN alpha other than that of IFN gamma or TNF alpha on HPRE . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Results indicate that dendritic cells , macrophages , and B cells from 3 day old mice respond to CpG stimulation by secreting IFN gamma , IL 12 , and / or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines such as Interleukin 1beta , ( IL 1beta ) , IL 2 , IL 3 , IL 6 , Interferon gamma ( IFN gamma ) and Tumor Necrosis Factor alpha ( TNF alpha ) were measured by Enzyme Linked Immunosorbent Assay ( ELISA ) in the homogenates of spleen tissue , lymph nodes and also in serum of AOS mice and compared with that of control mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines , released during the gastric inflammatory response , including IFN gamma , TNF alpha and IL 1 beta stimulate the G cell to produce gastrin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The supernatant of rhPRL stimulating NK cells inhibited the proliferation of HT 29 cells through , at least partly , the interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) in the supernatant . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IEL cytokine mRNA expression was also significantly altered with TPN : IL 2 and IL 10 expression declined , and IL 4 IL 6 , interferon gamma ( IFN gamma ) , transforming growth factor beta 1 ( TGF beta 1 ) , and tumor necrosis factor alpha ( TNF alpha ) were increased , when compared with Control or TPN+Food mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The activities of TNF alpha and IFN gamma were measured by biological methods . ^^^ The mRNA of TNF alpha , IFN gamma , T bet , a Th 1 specific transcription factor and Fas was detected by reverse transcript polymerase chain reaction ( RT PCR ) . ^^^ Compared with naive mice , the arthritic mice showed an increased IFN gamma and TNF alpha production in serum , and the spleen cells from the arthritic mice displayed a Th 1 cytokine profile and directly damaged bone cells in vitro . ^^^ Moreover , the activities of TNF alpha , IFN gamma and MMP 9 but not the production of NO were higher in joint tissues of arthritic mice than those of naive mice . ^^^ TNF alpha and IFN gamma significantly elevated the activity of MMP 9 in spleen cells from both arthritic and naive mice in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma and TNF alpha are involved in urushiol induced contact hypersensitivity in mice . ^^^ IFN gamma , TNF alpha and IFN gamma inducible protein 10 ( IP 10 ) mRNA were expressed after challenge of the antigen in urushiol sensitized mice , but not in unsensitized mice . ^^^ IFN gamma knockout ( KO ) mice and TNF alpha KO mice failed to elicit CHS with urushiol . ^^^ Contact hypersensitivity and expressions of IFN gamma , TNF alpha and IP 10 mRNA were markedly suppressed in CD4+ and CD8+ cell depleted mice . ^^^ These results suggest that IFN gamma , TNF alpha , and possibly IP 10 , play a critical role in CHS induced by urushiol , depending on both CD4+ T cells and CD8+ T cells . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Other genes dysregulated in F 1 CH C , albeit less markedly than IFN alpha / beta and IFN gamma inducible genes , were mainly involved in the activation of lymphocytes infiltrating the liver ( IFNG , TNF , CXCL 6 , IL 6 , CCL 8 , CXCR 3 , CXCR 4 , CCR 2 ) , cell proliferation ( p16 / CDKN2A , MKI 67 , p14 / ARF ) , extracellular matrix remodeling ( MMP 9 , ITGA 2 ) , lymphangiogenesis ( XLKD1 / LYVE ) , oxidative stress ( CYP2E1 ) , and cytoskeleton microtubule organization ( STMN2 / SCG10 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
All immunized macaques but one gave a good cross reactive antibody response to Tat but the proliferative response and levels of IL 2 , IFN gamma and TNF alpha secretion of peripheral blood mononuclear cells ( PBMCs ) recalled ex vivo with active Tat protein were weak . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition to mediating ADCC , engagement of the FcR on self specific CD 8 ( + ) T cells results in the production of both IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Activation of macrophages with recombinant IFN gamma or TNF alpha before infection altered the concentrations of elements in the phagosome , which was not observed in cells activated following infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CSF IL 6 , IFN gamma , TNF alpha , and IL 8 were significantly higher in survivors compared with nonsurvivors . ^^^ There were negative correlations between log TNF alpha , IFN gamma , and IL 6 levels and baseline cryptococcal CFU . ^^^ Log IFN gamma , G CSF , TNF alpha , and IL 6 were correlated positively with the rate of fall in log CFU / ml CSF / day . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Synergistic effect of LPS and ( TNF alpha + IFN gamma ) on pregnancy loss . ^^^ METHODS : To test the role of LPS in spontaneous and cytokine boosted abortions , recombinant TNF alpha + IFN gamma , and LPS were injected in different doses and sequences intraperitoneally ( i . p . ) into CBA 10 DBA / 2 mated mice in the Toronto General Research Institute animal facility where the endogenous abortion rate is < 30 % . ^^^ The effects of poly IC , a tlr 3 agonist that induces IFN gamma that can reverse LPS induced tolerance , and effects of anti MD 1 on TNF alpha induction by LPS , poly IC , CPG , or HSP in vitro were also examined . ^^^ The magnitude by which the abortion rate could be increased by an i . p . injection of 2000 u TNF alpha + 1000 u IFN gamma on gd 7 . 5 was independent of the endogenous rate of loss , and could not be increased by doubling the dose . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
ICV injection of IFN gamma or TNF alpha , but not IL 6 or IL 12 , also induced leukocyte infiltration into the brain . ^^^ Injection of IL 1beta , IFN gamma , TNF alpha , IL 6 , and IL 12 induced IL 1beta expression in the brain , with IL 6 and IL 12 being the least effective . ^^^ Leukocyte infiltration induced by icv IFN gamma and TNF alpha was also abrogated in IL 1R1 knockout animals . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We show that intradermal vaccination with recombinant poxviruses activated Valpha14iNKT cells and NK cells in the livers of BALB / c mice while inducing IFN gamma and tumor necrosis factor alpha ( TNF alpha ) producing pre erythrocytic stage antigen specific CD8+ T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
SPMG treatment at 5 and 10 mg / kg enhanced concanavalin A ( ConA ) induced T cell proliferation , cellular levels of CD 69 , interleukin 2 ( IL 2 ) , and interferon gamma ( IFN gamma ) , as well as CD4 / CD8 ratio , while decreasing tumor necrosis factor alpha ( TNF alpha ) level in T cells of peripheral blood mononuclear cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Measurements included plasma steroid kinetics and IL 10 concentrations and responses to LPS including proinflammatory cytokines ( TNF alpha , IFN gamma ) and circulatory NO measured as plasma nitrate / nitrite concentrations . ^^^ The intraperitoneal dosing of LPS produced large and transient elevations of plasma TNF alpha , IFN gamma , and NO concentrations . ^^^ The production of NO was described as a cascading consequence of the TNF alpha and IFN gamma plasma concentrations . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Research has indicated that physiologic connections between IFN gamma and TNF alpha are suggestive of a connection between the symptoms associated with schizophrenia and those of hypoglycemic events in IDDM . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
M ( 2 ) muscarinic and alpha ( 1B ) and alpha ( 2B ) adrenergic receptors expression was upregulated when cells were treated with the proinflammatory cytokines IFN gamma and / or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 4 , IFN gamma , and TNF alpha were undetectable . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Maturation of moDCs with either PGE 2 or PGA 2 resulted in enhanced IFN gamma , TNF alpha , and IL 5 production and repressed IL 10 production in allogeneic mixed leukocyte cultures . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The number of interleukin 4 ( IL 4 ) , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) secreting cells were measured by ELISPOT assay . ^^^ Based on IFN gamma and TNF alpha release , there was a trend toward a decrease in the number of cytokine secreting cells in PBSCT compared with BMT . ^^^ Both IFN gamma secreting cells and TNF alpha secreting cells showed a trend to increase in number in patients with acute GVHD . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The purpose of this study was to determine whether a cause and effect relationship might exist among proinflammatory cytokines , IL 1beta , TNF alpha , IFN gamma , or transforming growth factor beta ( TGF beta ) and hyaluronan expression in human JDMC and , if so , to identify possible mechanisms involved in the induction of hyaluronan expression . ^^^ TGF beta , TNF alpha , and IFN gamma had little or no effect on hyaluronan production by these cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Most studies have demonstrated the association of circulating levels of interferon gamma ( IFN gamma ) , tumour necrosis factor a ( TNF alpha ) , interleukin 10 ( IL 10 ) , transforming growth factor ( TGF beta ) and soluble Tumour Necrosis Factor Receptors ( sTNF RI and sTNF RII ) with the morbidity of malaria . ^^^ In contrast , co infected adults presented a significant increase of IFN gamma , IL 10 , TGF beta , sTNF RI and sTNF RII rates and IL 10 / TNF alpha ratio . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The ODN ID 35 showed levels of activity comparable with those induced by the murine prototype ODN 1826 in B cell proliferation , CD86 / CD69 expression , interleukin ( IL ) 6 , IL 12 , IL 18 , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) mRNA expression and IFN gamma / IL 12p70 protein production assays . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Barrier dysfunction was also induced by TNF alpha addition to IFN gamma primed , but not control , Caco 2 monolayers . ^^^ TNF alpha treatment of IFN gamma primed monolayers caused increases in both MLCK expression and MLC phosphorylation , suggesting that MLCK is a TNF alpha inducible protein . ^^^ These data show that IFN gamma can prime intestinal epithelial monolayers to respond to TNF alpha by disrupting tight junction morphology and barrier function via MLCK up regulation and MLC phosphorylation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Among IL 1beta , TNF alpha , and IFN gamma , only IL 1beta alone was capable of inducing iNOS . ^^^ All three cytokines alone induced the activation of AP 1 while IL 1beta and TNF alpha but not IFN gamma induced the activation of NF kappaB . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Concomitantly , pre treatment vs . 6 month concentrations of ELF IFN gamma and TNF alpha were decreased ( P < 0 . 05 ) , while those of IL 4 and IL 10 were increased ( P < 0 . 012 ) . ^^^ At serum level , IFN gamma decreased , as did TNF alpha , TGF beta 1 and PDGF BB ( P < 0 . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CXCR 3 memory B cells up regulate CXCR 3 and migrate toward concentration gradients of its ligands only when costimulated with interferon gamma ( IFN gamma ) , but not interleukin 4 ( IL 4 ) , IL 1beta , IL 6 , IFN alpha , IFN beta , or tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CD 40 is expressed by beta cells , and its expression is upregulated by proinflammatory cytokines ( IL 1beta , IFN gamma and TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To this end , diluted whole blood collected from 17 normal volunteers was incubated with fluoxetine ( 10 ( 6 ) and 10 ( 5 ) M ) , with or without SQ 22536 ( 10 ( 6 ) and 10 ( 4 ) M ) and Rp 8 Br cAMPs ( 10 ( 6 ) and 10 ( 4 ) M ) , afterwards , IFN gamma , IL 10 and the tumor necrosis factor alpha ( TNF alpha ) were determined . ^^^ Fluoxetine , 10 ( 6 ) and 10 ( 5 ) M , significantly reduced the production of IFN gamma and TNF alpha , and significantly decreased the IFN gamma / IL 10 production ratio . ^^^ It is concluded that the suppressant effect of fluoxetine on the IFN gamma / IL 10 production ratio is probably not related to the induction of the cAMP dependent PKA pathway , whereas the suppressant effect on TNF alpha may be related to the induction of PKA . ^^^ The obtained results suggest that increased activation of the PKA dependent pathway may constitute an important molecular basis for some ( suppression of TNF alpha production ) , but not all ( suppression of IFN gamma production ) , negative immunoregulatory effects of fluoxetine . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The role of IFN gamma and TNF alpha responsive regulatory elements in the synergistic induction of indoleamine dioxygenase . ^^^ In human epithelial cells , interferon gamma ( IFN gamma ) induced IDO expression is transcriptionally enhanced by tumor necrosis factor alpha ( TNF alpha ) . ^^^ Nuclear concentrations of signal transducer and activator of transcription 1 ( Stat 1 ) and IFN regulatory factor 1 ( IRF 1 ) , transcription factors that bind gamma activated sequences ( GAS ) and IFN stimulated response elements ( ISRE ) , respectively , were found to increase after stimulation with IFN gamma and TNF alpha relative to stimulation with individual cytokines . ^^^ Additionally , CCAAT enhancer binding protein beta ( C / EBP beta ) bound to one of three consensus C / EBP beta sites in the IDO regulatory region in response to TNF alpha alone or combined with IFN gamma . ^^^ A transcriptional reporter containing green fluorescent protein ( GFP ) under the control of the IDO regulatory region was used to analyze the contribution of these enhancer elements to synergistic IDO gene expression in response to IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We report that VIP inhibited production of the proinflammatory cytokines , tumor necrosis factor alpha ( TNF alpha ) and interleukin 1beta ( IL 1beta ) , at the mRNA level and that the inhibitory effect of VIP was maintained when macrophages were cocultured with an immunostimulatory concentration of interferon gamma ( IFN gamma ) ( 100 U / ml ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Strain specific TNF alpha , IFN gamma , IL 6 and IL 10 responses in naive animals were not associated with survival after rat liver transplantation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cell homogenates were incubated with Trans Signal Angiogenesis Antibody Array containing antibodies to angiogenic activators ( ANG , HGF , leptin , VEGF , IL 6 , IL 8 , PIGF , FGF alpha / beta , TNF alpha and TGF alpha ) and inhibitors ( IFN gamma , IL 12 , IP 10 , TIMP 1 and 2 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Soluble interleukin 1 receptor antagonist ( sIL 1RA ) , IL 2 , sIL 2R , IL 3 , IL 4 , IL 6 , sIL 6R , IL 8 , IL 10 , transforming growth factor beta 2 ( TGF beta 2 ) , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) levels were determined using commercially available enzyme linked immunosorbent assay ( ELISA ) kits . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN gamma ) induced endothelial cell GTPCH 1 protein and BH 4 modestly , whereas high level induction required combinations of IFN gamma and tumor necrosis factor alpha ( TNF alpha ) . ^^^ In the presence of IFN gamma , TNF alpha increased GTPCH 1 mRNA in a manner dependent on nuclear factor kappaB ( NF kappaB ) , as this effect was abrogated by overexpression of a dominant negative IkappaB construct . ^^^ Conversely , overexpression of Jak 2 effectively substituted for IFN gamma in supporting TNF alpha mediated GTPCH 1 induction . ^^^ Thus , IFN gamma and TNF alpha exert distinct but cooperative roles for BH 4 biosynthesis in endothelium that may have important implications for vascular function during vascular inflammation . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , the treatment with pioglitazone decreased the expression levels of proinflamatory cytokine ( TNF alpha and IL 1beta ) genes and Th 1 cytokine ( IFN gamma ) genes , and increased the expression levels of Th 2 cytokine ( IL 4 ) gene . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Modulation of TNF alpha gene expression by IFN gamma and pamidronate in murine macrophages : regulation by STAT 1 dependent pathways . ^^^ Furthermore , pretreatment of murine macrophages with pamidronate before stimulation with IFN gamma significantly augments IFN gamma dependent production of TNF alpha . ^^^ This pamidronate mediated augmentation of TNF alpha production results in sustained phosphorylation of the tyrosine residue at position 701 of STAT 1 after IFN gamma treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mechanistically , combined blockade of L selectin and MAdCAM 1 depleted the intestinal lamina propria of CD4+ T cells that aberrantly coexpressed alpha4beta7 and alpha4beta1 integrins , markedly decreasing local production of TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
T 4 treatment of young males inhibited proliferation of myelin oligodendrocyte glycoprotein 35 55 specific T cells and secretion of TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We examined IFN gamma , IL 4 , IL 5 , IL 8 , and IL 10 produced by T cells and TNF alpha , IL 1beta , IL 10 , and IL 12 produced by MoDCs using real time PCR analysis or by ELISA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Secretion of IFN gamma and TNF alpha by DC was detected by ELISA . ^^^ RESULTS : DC IFN gamma could secrete high levels of IFN gamma , NO and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Total RNA was extracted from the incubated macrophages , and then the levels of mRNA for induced nitric oxide synthase ( iNOS ) , IL 1beta , TNF alpha , GM CSF , and IFN gamma were detected . ^^^ The results showed that expression of mRNA for iNOS , IL 1beta , TNF alpha , GM CSF , and IFN gamma decreased significantly compared to the positive control ( LPS only ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Other cytokines ( IL 2 and IL 6 ) were upregulated to a lesser extent ( 6 and 3 fold , respectively ) or not at all ( IL 1 , IL 8 , IFN gamma , and TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Levels of IL 6 , LIF , TNF alpha , IL 10 , IFN gamma , IL 16 and RANTES in the medium were measured by ELISA on days 1 , 4 and 7 of culture . ^^^ High levels of IL 6 and LIF production , low levels of TNF alpha , IL 10 and IFN gamma production and significant SDF 1 expression were observed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : IFN gamma and TNF alpha production after PPD stimulation was markedly decreased in HIV patients , but not in HIV / TB patients . ^^^ In HIV patients with a CD 4 cell count of less than 200 / mm3 , IFN gamma and TNF alpha production after PPD stimulation was higher in HIV / TB patients than in HIV patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Such toxicity was induced by the following combinations : interferon gamma ( IFN gamma ) with tumor necrosis factor alpha ( TNF alpha ) ; IFN gamma with interleukin ( IL ) 1alpha or IL 1beta in the presence of TNF alpha ; and IL 6 with TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Genotyping was carried out for HLA DRB 1 and HLA DQB 1 and for polymorphism of the genes encoding TNF alpha ( 238 , 308 , 376 ) , TGF beta ( codon 10 and 25 ) , IFN gamma ( +874 ) , IL 6 ( 174 ) , IL 10 ( 592 , 819 , 1082 ) , CTLA 4 ( 318 , +49 ) , and the ( AT ) ( n ) repeats of the CTLA 4 gene . ^^^ RESULTS : An increase in the IFN gamma +874 T / T ( odds ratio ( OR ) = 3 . 14 ) and TNF alpha 238 G / A ( OR = 5 . 01 ) genotypes was found in WG patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha , TGF beta and IFN gamma were determined using ELISAs . ^^^ TNF alpha and IFN gamma were higher in AIH 1 ( P=0 . 003 and P=0 . 001 ) and PBC ( P=0 . 0001 ) compared to controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MUC 1 expression on activated T cells was increased in the presence of IL 12 but was not affected by IFN gamma , IL 2 , IL 4 , IL 5 , IL 10 , IL 13 or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Increase of TNF alpha , IL 4 , NO level , and decrease of IFN gamma level in bronchoalveolar lavage fluid ( BALF ) and cerebral cortex in sensitized rats were shown after repeated antigen challenge . ^^^ Ketotifen also inhibited the increase of TNF alpha level and 5 LO pathway enzyme expression in lung and cerebral cortex , but had no effect on the level of NO , IL 4 , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Supernatants were harvested to evaluate tumor necrosis factor alpha ( TNF alpha ) , interleukin 10 ( IL 10 ) , transforming growth factor beta ( TGF beta ) and interferon gamma ( IFN gamma ) production by ELISA . ^^^ Results show that the blockade of CD 28 B7 interaction by CTLA 4 Ig downmodulated IFN gamma , IL 10 , and TNF alpha secretion by PBMC from CL patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We observed increased expression of IL 1beta , TNF alpha , IFN gamma , MCP 1 , RANTES , and IP 10 in C . neoformans infected brains of immune mice compared to control mice suggesting that these cytokines and chemokines are associated with the protective immune response . ^^^ Furthermore , the Th 1 type cytokines TNF alpha and IFN gamma , but not the Th 2 cytokines IL 4 and IL 5 , were secreted at significantly higher levels in C . neoformans infected brains of immune mice compared to control mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
HPV infection was associated with a marked increase in cells expressing interleukin ( IL ) 6 , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) . ^^^ Coinfection by HPV and HIV 1 led to decreased expression of IL 6 , TNF alpha , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both MyD 88 ( / ) and TLR 2 ( / ) animals showed decreased TNF alpha , IL 12p40 and / or IFN gamma expression in various organs during infection . ^^^ In conclusion , our data indicate that TLR 2 and MyD 88 , but not TLR 4 , critically contribute to anti cryptococcal defenses through the induction of increased TNF alpha , IL 12 and IFN gamma expression . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : A colonic cell line ( HT 29 ) was stimulated for 1 10 weeks with interferon gamma ( IFN gamma ) or tumour necrosis factor alpha ( TNF alpha ) or both and compared with unstimulated cells or cells stimulated for 48 h . ^^^ RESULTS : Continuously stimulated colonic cells had increased ROS production , especially those stimulated with TNF alpha or IFN gamma / TNF alpha ( P < 0 . 001 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pro angiogenic cytokines , such as IL 1 and TNF , and anti angiogenic cytokines such as IFN gamma and IL 12 , will be briefly described . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
While in contrast to ferritin overexpression , single administration of the CO donor methylene chloride ( MC ) or of BV also protected mice from liver damage , only coadministration of both HO products prolonged survival and reduced the expression of cytokines , e . g . , tumor necrosis factor ( TNF ) and interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MA enabled production of IFN gamma and myeloperoxidase , enhanced TNF alpha production and suppressed IL 10 upon renewed exposure to innate triggers . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here , we report that unlike Th 1 cytokines ( TNF alpha , IFN gamma ) , the Th2 / 3 cytokine TGF beta induces primary microglia from C57BL / 6 mice to secrete a chemotactic factor for primary OPC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Canine cytokine mRNA expression in peripheral blood mononuclear cells collected prior to treatment and at the completion of the study was determined for IFN gamma , IL 4 , TNF and IL 10 genes using quantitative reverse transcription competitive polymerase chain reaction . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Involvement of TNF and NF kappa B in the transcriptional control of cyclooxygenase 2 expression by IFN gamma in macrophages . ^^^ Interestingly , IFN gamma induction of the COX 2 and PGE ( 2 ) synthesis was absent in macrophages from TNF ( / ) mice , and neutralizing anti TNF Abs inhibited COX 2 promoter induction by IFN gamma in RAW 264 . 7 macrophages . ^^^ Moreover , NF kappaB activity was induced late after stimulation with IFN gamma correlating with the effect of autocrine TNF , and this NF kappaB activation was absent in macrophages from TNF ( / ) mice . ^^^ Taken together our results suggest a model in which IFN gamma induced TNF activates NF kappaB , which is required for full COX 2 expression . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Islets and beta cell lines were treated with a combination of IL 1beta , IFN gamma and TNF alpha , or the 12LO product 12 ( S ) hydroxyeicosatetraenoic acid ( HETE ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we compared the effects of TNF alpha , interferon ( IFN ) gamma , IL 1beta or IFN gamma + IL 1beta on the phenotypic and functional maturation of DCs . ^^^ Furthermore , IFN gamma + IL 1beta treated DCs produced larger amounts of IL 12 and induced stronger T cell proliferation and IFN gamma secretion in primary allogeneic mixed lymphocyte reaction ( MLR ) than did TNF alpha treated DCs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study , real time reverse transcriptase polymerase chain reaction ( RT PCR ) assays were employed to quantify messenger RNA ( mRNA ) encoding the chemokines monocyte chemotactic protein ( MCP ) 2 , eotaxin 2 and eotaxin 3 , and the cytokines interleukin 4 ( IL 4 ) , IL 5 , IL 6 , IL 10 , IL 12p40 , IL 18 , interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) and transforming growth factor beta ( TGF beta ) in normal nasal , bronchial and pulmonary tissues from puppies ( n = 4 ) and from adult dogs ( n = 7 ) . ^^^ Transcripts encoding IL 6 , IL 10 , IL 12 and TNF alpha were approximately ten fold less abundant , and IL 4 , IL 5 and IFN gamma were the least abundant templates . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We demonstrate that primer / probe systems for IL 4 , IL 10 , IFN gamma , TNF alpha , GAPDH , and the pol region of PLV 1695 amplify puma cytokines and PLV 1695 with high amplification efficiency and sensitivity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Monoclonal Abs producing scattergrams with a clear demarcation between positive and negative cell populations were found within the anti IL 2 , IL 6 and IFN gamma panels , whereas less well defined positive and negative cell populations could be generated by use of mAbs within the anti IL 4 and TNF alpha panels . ^^^ Data presented are given as mean + / SD of the percentage of positive staining lymphocytes : IL 2 , 45 . 5 + / 27 . 6 ; IL 4 , 34 . 1 + / 21 . 3 ; IL 6 , 45 . 4 + / 23 . 8 ; IL 12 , 13 . 9 + / 8 . 6 ; TNF alpha , 43 . 4 + / 11 . 3 ; IFN gamma , 65 . 5 + / 14 . 8 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 10 DC and TGF beta DC resulted in lower nonspecific ( Con A induced ) and antigen specific ( ovalbumin stimulated ) spleen mononuclear cells proliferation , accompanied by lower levels of IFN gamma , IL 10 , and TNF alpha . ^^^ On the contrary , IFN gamma DC did not suppress cell proliferation and IFN gamma and IL 10 production except only slightly decreased TNF alpha levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A fraction of tetramer positive CD8+ T cells produce cytokines ( IFN gamma , TNF alpha ) or chemokines ( CCL 4 , CCL 5 ) after ex vivo stimulation with the cognate peptide . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Macrophages were examined ex vivo from mice orally treated with various doses of MCZ for 5 consecutive days per week for 4 weeks ( subacute exposure , 250 , 1000 , 1500 mg / kg / day ) followed by culture for 2 ( TNF alpha ) or 3 days ( NO ) in the presence of LPS plus IFN gamma . ^^^ Macrophages from naive mice were also cultured with various concentrations of MCZ ( 0 . 05 , 0 . 25 , 0 . 5 , 1 and 2 microg / mIL in the presence of LPS plus IFN gamma for 2 ( TNF alpha ) or 3 days ( NO ) in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma / GM CSF induced TNF release , and co culture with LPS 1 . 0 or 10 . 0 ng / mL was strongly synergistic . ^^^ CARD 15 1007fs mutation was linked in a gene dose dependent manner to low TNF release induced by IFN gamma / GM CSF ( P value for linear trend = 0 . 001 ) . ^^^ CONCLUSIONS : In CD patients , CARD 15 1007fs is associated in a gene dose dependent manner to low mononuclear cell TNF release by IFN gamma / GM CSF but does not impair TNF release by LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
While the APC function of PB pDCs was less efficient than that of PB myeloid DCs , RA SF pDCs efficiently activated resting allogeneic PB T cells , and high levels of IFN gamma , IL 10 , and tumor necrosis factor alpha were produced in response to incubation of allogeneic T cells with either type of SF DCs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CGS greatly reduced Tc 1 and Tc 2 cell interleukin 2 ( IL 2 ) and tumor necrosis factor alpha ( TNF alpha ) secretion , with nominal effect on interferon gamma ( IFN gamma ) secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : IL 15 , in the absence of Io + PMA , did not induce the expression of IFN gamma , TNF alpha , or IL 2R alpha . ^^^ Only inflamed explants from resections stimulated with Io + PMA expressed IFN gamma , TNF alpha , and IL 2R alpha . ^^^ In rectal biopsy explants , inflamed , noninflamed CD , and control tissue responded to stimulation with Io + PMA ( P < 0 . 05 ) with increased IFN gamma and TNF alpha ( P < 0 . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We then developed primers to be used under one single experimental condition for the specific amplification of human IL 1beta , IL 4 , IL 10 , IL 12p40 , IL 13 , IL 15 , IL 18 , IFN gamma , MIF , TGF beta 1 and TNF alpha mRNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Additionally , the effects of 17beta estradiol on basal and LPS induced release of cytokines ( TNF alpha , IFN gamma , IL 2 , IL 4 , and IL 10 ) were determined . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We challenged ECs with cytokines that elicit different patterns of inflammatory and immune responses in immune cells : tumor necrosis factor ( TNF alpha ) , interferon gamma ( IFN gamma ) or interleukin 4 ( IL 4 ) , and used microarrays containing approximately 40 , 000 unique cDNAs , to assess changes in differential gene expression relative to untreated cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Negligible induction of IFN gamma , IL 12 and TNF alpha by DNA PEI 750 kDa / albumin complexes . ^^^ Using as little as 1 mug DNA transferred in vivo caused an almost negligible response from pro inflammatory cytokines ( IFN gamma , IL 12 and TNF alpha ) , as assessed in serum with a commercially available kit . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF , IFN gamma , and endotoxin increase expression of DMT 1 in bronchial epithelial cells . ^^^ Treatment with TNF alpha , IFN gamma , or LPS increased both forms . ^^^ Twenty four hours after exposure of an animal model to TNF alpha , IFN gamma , or LPS , a significant increase in pulmonary expression of IRE DMT 1 was seen by immunohistochemistry ; the level of +IRE DMT 1 was too low in the lung to be visualized using this methodology . ^^^ Finally , iron transport into BEAS 2B cells was increased after inclusion of TNF alpha , IFN gamma , or LPS in the media . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We now report that HSP 60 in vitro differentially modulates the expression of Th1 / Th2 transcription factors in human T cells : HSP 60 down regulates T bet , NF kappaB , and NFATp and up regulates GATA 3 , leading to decreased secretion of TNF alpha and IFN gamma and enhanced secretion of IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the absence of WSX 1 , an increased production of the proinflammatory cytokines TNF and IL 12p40 resulted in elevated CD4+ T cell activation and IFN gamma production , which enhanced macrophage effector functions and reduced bacterial loads . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
At study entry and before the start of each dialysis session the following examinations were performed : CRP , albumin , fibrinogen , ferritin , transferrin , prealbumin and serum levels of IL 6 , IL 10 , IFN gamma , tumor necrosis factor alpha ( TNF alpha ) . ^^^ While IL 6 , IL 10 and IFN gamma were unchanged during the dialysis session , there was a significant variation in TNF alpha levels , which were increased in BHD ( from 10 . 9 + / 3 . 1 to 14 . 7 + / 4 . 1 pg / mL ; p=0 . 004 ) and reduced in PHF AF ( from 11 . 9 + / 2 . 8 to 6 . 3 + / 2 . 2 pg / mL ; p=0 . 0004 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We measured the circulating levels of TF , interleukin 6 ( IL 6 ) , IL 6 receptor ( sIL 6R ) , tumor necrosis factor ( TNF alpha ) and interferon gamma ( IFN gamma ) in 83 patients with autoimmune aPL ( 42 with and 41 without clinical features of definite primary APS ) , 48 leprosy patients ( 33 with aPL ) and 48 normal controls . ^^^ Elevated levels of IL 6 and TNF alpha and a trend to lower IFN gamma were found in patients with definite APS . ^^^ Leprosy patients with aPL , however , had increased TNF alpha and IFN gamma but normal IL 6 levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , MBP : : SSP 4 was also shown to induce the expression of IL 1alpha , IL 6 , IL 12 , IFN gamma , and TNF alpha in normal animals , and IL 10 in immunized animals . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In Leishmania infected dogs , interleukin 4 ( IL 4 ) , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) messenger RNA production was significantly higher than controls . ^^^ By comparing the pattern of inflammation and cytokine expression , a clear trend became evident in that levels of IL 4 , TNF alpha , and IFN gamma were elevated in biopsies with a periadnexal nodular pattern and in biopsies where the severity of the periadnexal infiltrate was equal to the perivascular to interstitial infiltrate . ^^^ Expression of IL 4 , IL 13 , and TNF alpha was slightly increased in biopsies where plasma cells prevailed on lymphocytes , whereas expression of IFN gamma was moderately higher when lymphocytes were predominating . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The analyses of the single Jirel pedigree revealed significant heritabilities for IFN gamma ( h 2 = 0 . 654+ / 0 . 096 ) , TNF alpha ( h 2 = 0 . 458+ / 0 . 101 ) , IL 2 ( h 2 = 0 . 583+ / 0 . 101 ) , IL 4 ( h 2 = 0 . 700+ / 0 . 095 ) , IL 5 ( h 2 = 0 . 676+ / 0 . 087 ) , and IL 10 ( h 2 = 0 . 597+ / 0 . 093 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
SOCS 1 , 2 and 3 mRNA expression was strongly increased in human islets after exposure , in vitro , to IFN gamma , IL 1beta and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of THP 1 cells with immobilized anti DR 3 monoclonal antibody in combination with IFN gamma caused induction of pro atherogenic cytokines / chemokines such as TNF alpha , monocyte chemoattractant protein ( MCP ) 1 , and interleukin ( IL ) 8 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This was followed by a significant increase in levels of IL 10 , whereas IFN gamma gene upregulation was more transient , and levels of TNF alpha and MIP 1alpha / beta transcripts did not increase in either blood or tissues . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The percentage of IL 10 and IL 12 , but not IFN gamma , IL 4 or tumour necrosis factor ( TNF ) alpha producing cells was significantly higher in patients compared to HC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Genetic polymorphism in the cytokines TNF alpha , IFN gamma , TGF beta 1 , IL 6 , and IL 10 were studied in 236 Japanese patients with HBV infection . ^^^ RESULTS : There was no statistically significant difference in the genetic polymorphisms of TNF alpha , IFN gamma , and IL 10 genes between HBV carriers with HCC and those without HCC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In process T cell activation was reproducibly robust , as measured by increase in cell size , up regulation of CD 25 and CD 154 expression and the secretion of IL 2 , IFN gamma and tumor necrosis factor ( TNF ) alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The present study aimed to reveal the correlation between serum levels of IFN gamma , IL 12 , TNF alpha and IL 10 , measured by enzyme immunoassay , and clearance of bacteria in stool of patients with gastroenteric Salmonella infection . ^^^ Mean serum concentrations of IFN gamma , IL 12 , TNF alpha and IL 10 in the acute stage were compared between the 2 groups of patients and with those of healthy controls . ^^^ In patients with early bacterial clearance mean serum concentrations of IFN gamma , IL 12 and TNF alpha in the acute stage were significantly ( p < 0 . 05 ) higher than in the non clearance group . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Initiation of ileitis in SAMP1 / YitFc mice was T H 1 mediated because up regulation of IFN gamma and tumor necrosis factor ( TNF ) preceded the histologic injury , whereas IFN gamma neutralization prevented the development of chronic inflammation ( P < . 005 ) by interfering with the expansion of lymphocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma and , in some models , other T cell cytokines such as TNF alpha , IL 4 or IL 10 contribute to tumor rejection by inhibition of tumor stroma formation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The more pronounced increments were observed in macrophage cultures exposed to fraction FII which demonstrated that ( a ) the highest levels of IL 1alpha , IL beta , and TNF were observed after 12 hours and that ( b ) the maximum levels of IFN gamma and NO were observed after 72 hours . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment with the KCNN 4 blocker did not prevent infiltration of T cells in the spinal cord , but resulted in the reduction of both the protein and the message levels of TNF alpha and IFN gamma as well as the message levels of several other pro inflammatory molecules in the spinal cord . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
At a dose of 100 mg / kg , ASA or ACET did not affect the pain behavior induced by TNF alpha ( 100 pg ) , IL 1beta ( 100 pg ) or IFN gamma ( 100 pg ) administered intrathecally . ^^^ However , at doses of 200 and 300 mg / kg , ASA or ACET significantly and dose dependently attenuated pain behavior induced by TNF alpha , IL 1beta or IFN gamma administered intrathecally . ^^^ Our results suggest that orally administered ASA and ACET produce antinociception by inhibiting the nociceptive action of TNF alpha , IL 1beta or IFN gamma administered intrathecally . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Indeed , while MS patients and mice subjected to experimental autoimmune encephalomyelitis ( EAE ) display gender specific alterations of IFN gamma and IL 12 , variations of TNF and IL 6 were associated with PD . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Concentration of tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) and interleukin 2 ( IL 2 ) in mice serum were measured in control and polysaccharide groups . ^^^ The concentration of TNF alpha , IFN gamma in serum increased significantly in the polysaccharide groups compared with the model control group , but IL 2 not . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RT PCR analysis of cytokine expression by the tetramer ( + ) T cells compared with tetramer ( ) cells indicated the tetramer ( + ) cells expressed high levels of Th 1 and proinflammatory cytokines , including IL 2 , IFN gamma , IL 6 , TNF alpha , and especially IL 17 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We show that although IL 12 cultured NK cells produced abundant IFN gamma , TNF alpha , and GM CSF in response to stimuli acting on the NKp 46 activating receptor , IL 4 cultured NK cells did not release detectable levels of these cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , the ability to overproduce TNF alpha and IFN gamma in response to LPS , lipid A , synthetic lipopeptide Pam ( 3 ) CysK ( 4 ) , or whole killed bacteria was present in P . acnes primed wild type , but not TLR 9 ( / ) , mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our results demonstrate that in the presence of APCs , beryllium induced strong proliferation responses of BAL CD 4 ( + ) T cells , production of superoptimal concentrations of secreted proinflammatory cytokines , IFN gamma , TNF alpha , and IL 2 , and up regulation of numerous T cell surface markers that would promote T T Ag presentation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
By Northern blot and promoter activity , we show that a cytokine mix ( TNF alpha , IL beta , and IFN gamma ) induces NOSII transcription after 6 h stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
More importantly , analysis of the cytokine responses revealed a reduction in the IFN gamma and TNF alpha production in the lungs of IgA / compared to IgA+ / + mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The serum levels of interferon gamma ( IFN gamma ) and interleukin 2 ( IL 2 ) were increased , while no significant increase was observed in interleukin 4 ( IL 4 ) , interleukin 6 ( IL 6 ) , interleukin 10 ( IL 10 ) or tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Gamma interferon ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) and interleukins 2 , 4 and 6 ( IL 2 , IL 4 , IL 6 ) in cervical uterine cells of intraepithelial neoplasia : a preliminary report ] . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lymphoproliferation and cytokine responses IL 13 , IFN gamma , IL 6 , IL 10 , TNF alpha ) were measured for all of the cultures described above . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
All genotyping ( TNF alpha , TGF beta , IL 10 , IL 6 , IFN gamma ) experiments were performed using sequence specific primers PCR ( PCR SSP ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Genes for interleukin 5 ( IL 5 ) , IL 13 , and tumor necrosis factor alpha ( TNF alpha ) , but not for IL 4 , IL 10 , or gamma interferon ( IFN gamma ) , were consistently expressed at higher levels in both afferent and efferent lymph cells of R sheep than in S sheep . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Peripheral blood mononuclear cells were kept in short term culture with endotoxin free casein fractions and IFN gamma , TNF alpha , IL 5 , IL 10 cytokine producing cells were detected by ELISpot . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The induction of increased mRNA levels for all chemokines by SFLLRN was unsurpassed by LPS , zymosan , interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and IL 1 . ^^^ Activation of monocytes through PARs represents an alternate activation mechanism , independent from IFN gamma , TNF alpha , or other signaling pathways . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There were significant increases in LPS , SA , and GBS induced production of TNF alpha and IFN gamma in splenocytes from Galpha ( i 2 ) / mice compared with wild type ( WT ) mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When stimulated antigen specifically or non specifically in vitro , mice showed elevated thymidine uptake and a shift of cytokine secretion profiles toward Th 1 , i . e . , elevated levels IL 2 , TNF alpha , and IFN gamma , and lowered level of the Th 2 cytokine IL 4 , only when Vitamin C was administered during sensitization . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma primes macrophages for enhanced TNF alpha expression in response to stimulatory and non stimulatory amounts of microparticulate beta glucan . beta ( 1 > 3 ) D Glucan is an integral cell wall component of a variety of fungi , plants , and bacteria . ^^^ IFN gamma treatment alone did not induce unstimulated macrophages to produce TNF alpha . ^^^ However , a 4 h IFN gamma pretreatment augmented TNF alpha secretion by peritoneal macrophages subsequently treated with an optimally stimulatory dose of MG . ^^^ IFN gamma pretreatment for 2 h followed by thorough washing and a further 2 h incubation without IFN gamma still resulted in enhanced TNF alpha production in response to MG , suggesting that IFN gamma can prime macrophages for a subsequent proinflammatory response . ^^^ Most interestingly , we found that IFN gamma pretreatment of peritoneal macrophages enhanced the TNF alpha response to amounts of MG that were poorly stimulatory or non stimulatory in the absence of IFN gamma priming . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The aim of this study was to examine the synergistic effects of PHA plus low dose IFN gamma on TNF alpha mRNA production , cytosolic levels , and secretion in RAW 264 . 7 cells . ^^^ The optimum time of PHA for the TNF alpha mRNA production and release were 6 and 7 h after stimulation , respectively , whereas the time of IFN gamma on them was achieved at 3 and 8 h . ^^^ Although the TNF alpha mRNA production , cytosolic levels , and secretion from the cells were slightly detected under 10 microg / ml PHA and 1 ng / ml IFN gamma , the combination of PHA ( 10 microg / ml ) and IFN gamma ( 1 ng / ml ) greatly increased them , indicating the synergistic effect of PHA plus low dose IFN gamma on TNF alpha expression . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Semi quantitative reverse transcriptase polymerase chain reaction ( RT PCR ) and real time PCR showed a ConA induced increase of interferon gamma ( IFN gamma ) mRNA expression but not of interleukin 1beta ( IL 1beta ) , tumor necrosis factor alpha ( TNF alpha ) and IL 12 p 40 mRNA in feline PBMC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , the Th 1 cytokine profile ( IFN gamma and TNF alpha ) was not affected by infection , whilst progesterone treatment increased the expression of both cytokines two fold compared to uninfected , infected and placebo treated mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Before anti TNF therapy , whole blood cultures from RA patients released significantly less IFN gamma than healthy controls after stimulation with all tested microorganisms . ^^^ Although short term therapy with anti TNF agents did not further decrease the release of other proinflammatory cytokines , the combination of defective IFN gamma production in basal conditions and TNF neutralization during anti TNF therapy is likely to be responsible for the higher susceptibility to infections in patients with RA . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Therefore , H 35 rat hepatoma cells were employed to measure the effect of recombinant rat IL 1beta , IL 6 , IFN gamma , and TNF alpha on C 3 protein secretion and C 3 mRNA levels quantified by ELISA and quantitative RT PCR . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Clinical efficacy and T lymphocyte subset , serum interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 2 ( IL 2 ) levels on treatment of chronic aplastic anemia patients by shenfu injection combined with stanozol and cyclosporin A ] . ^^^ OBJECTIVE : To observe the effect of Shenfu injection ( SFI ) and influence on T lymphocyte subset , serum level of interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 2 ( IL 2 ) in patients with chronic aplastic anemia ( CAA ) based on treating with stanozol and cyclosporin A . ^^^ The change of T lymphocyte subset analyzed by flow cytometry and the levels of serum IFN gamma , TNF alpha , IL 2 measured with ELISA method were also observed before and after treatment . ^^^ CONCLUSION : SFI might believe the hemopoietic inhibition so as to promote the recovery of hemopoietic function through improving the T lymphocyte subset and reducing the release of hemopoietic negative regulatory factors such as IFN gamma , TNF alpha and IL 2 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Proinflammatory cytokines in particular , such as TNF , IL 6 , IL 18 or IFN gamma , may play a major role in propagating the inflammatory processes responsible for tissue damage . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We show that IFN gamma blocks hepatocyte proliferation in vivo , and that in combination with either tumor necrosis factor ( TNF ) or lipopolysaccharide ( LPS ) , it causes cell cycle arrest in hepatocytes but stimulates oval cell proliferation in cultured cells . ^^^ Treatment of AML 12 hepatocytes with IFN gamma / LPS or IFN gamma / TNF , but not with individual cytokines , induced NO synthase and generated NO , while similarly treated oval cells produced little if any NO . ^^^ In conclusion , we propose that IFN gamma , in conjunction with TNF or LPS , can both inhibit hepatocyte proliferation through the generation of NO and stimulate oval cell replication . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Functional studies showed that 5 HT modulates the release of IL 1beta , IL 6 , IL 8 / CXCL8 , IL 12p40 and tumor necrosis factor alpha ( TNF alpha ) , while it has no effect on the production of IL 18 and IFN gamma in LPS stimulated human blood monocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We examined plasma concentrations of interferon gamma ( IFN gamma ) , interleukin 10 ( IL 10 ) , soluble interleukin 2 receptor ( sCD 25 ) and soluble tumor necrosis factor receptor 2 ( sTNFR 2 ) in previously healthy infants during the acute phase of primary ADV infection ( n = 21 ) and RSV infection ( n = 68 ) , and in uninfected controls ( n = 44 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Following surface staining ( CD 3 Cy5+CD8 FITC ) , fixation and permeabilization , cells were stained with PE labelled antibodies against Th 1 cytokines ( IL 2 , IFN gamma , TNF alpha ) and Th 2 cytokines ( IL 4 , IL 10 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Freshly isolated PBMC were cultured for 4 or 24 h in 10 VIVO medium in the presence or absence of 0 . 01 microg / mL lipopolysaccharide for the determination of TNF alpha and IL 6 production ; alternatively , cells were incubated 24 h in 10 VIVO medium with or without 25 microg / mL Concanavalin A for IFN gamma production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The role of the immune response in the severity of RSV infection was examined by determining plasma concentrations of interferon gamma ( IFN gamma ) , interleukin 10 ( IL 10 ) , interleukin 2 receptor ( sCD 25 ) and soluble tumor necrosis factor receptor 2 ( sTNFR 2 ) in 196 , previously healthy infants , during acute and convalescence phases of primary RSV infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We quantified the capacity of IEL subsets to produce IFN gamma , TNF alpha , IL 2 , IL 4 , and IL 10 by intracellular staining by flow cytometry . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It was induced by IFN gamma treatment , but not by TNF alpha or TGF beta 1 treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RT PCR analysis showed that the allografts treated with CsA or KRP 203 alone showed no suppression of IL 10 , IFN gamma , and TNF alpha mRNA expression , but when combined with a subtherapeutic dose of CsA it completely suppressed their mRNA expressions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The present study has examined whether administration of L carnitine to young female mice alters the percentage of immune cells in peritoneal exudates and the uterus as well as the levels of IFN gamma , TNF alpha , IL 2 , IL 4 , IL 6 , VEGF , GM CSF and IGF 1 in blood serum , peritoneal fluid and supernatants of uterine cultured cells as tested by immunofluorescence or ELISA techniques , respectively , leading to a pathological disorder resembling human endometriosis . ^^^ The results showed that , except from infertility , L carnitine treatment resulted in a significant increase of macrophages and to a lesser degree an increase of T cells , while elevated levels of IFN gamma and TNF alpha were detected in both serum and peritoneal fluid compared to controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
As cytokine gene polymorphisms may be associated with different rates of cytokine production , the aim of the present study was to investigate the bi allelic polymorphisms in TNF alpha 308 G > A , TNF beta +252 G > A , IFN gamma +874 A > T genes as Th 1 or pro inflammatory factors as well as IL 4 590 C > T , IL 10 592 C > A , 819 C > T , 1082 A > G genes as Th 2 cytokines in women with RPL compared with healthy women . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We conclude that Ctx elicits a proinflammatory phenotype in intestinal epithelia and that potentiation of IFN gamma mediated barrier disruption by TNF alpha may contribute to the overall adjuvant properties of Ctx . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Infection of IL 12p40 ( / ) mice induced changes in the gene expression of the cytokines IFN gamma , IL 4 , IL 15 , IL 18 , TNF alpha and TGF beta during primary infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The present study reports the cytokine response ( TNF alpha , IFN gamma , IL 4 , IL 10 and TGF beta ) in such subjects as measured by RT PCR . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Monitoring peripheral blood during the acute response showed expression of IFN gamma and TNF alpha , with downregulation of IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OUTCOME MEASURES : We correlated determined circulating levels of IL 2 , sIL 2R , IL 4 , sIL 4R , IL 6 , sIL 6R , IL 1beta , IL 8 , IFN gamma , TNF alpha , sTNF RI , sTNF RII and RANTES in the mothers before delivery ( MS ) , the fetuses ( UC ) and the neonates in days 1 ( N 1 ) and 4 ( N 4 ) of life , with the mode of delivery . ^^^ RESULTS : sIL 2R in N 1 and N 4 , sIL 4R in MS , IL 6 in MS and UC , IL 1beta in MS , UC and N 1 , IFN gamma in MS and UC , TNF alpha in UC , N 1 and N 4 , sTNF RI in UC were significantly higher in cases of vaginal delivery than in cases of elective cesarean section ( p ranging from 0 . 0005 to 0 . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The resulting effector cells are skewed toward a type 1 phenotype and produce high levels of IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Upon intranasal infection of wild type mice , the lung weight is increased ; the cytokines TNF , IL 12p40 , and IFN gamma , as well as the chemokines keratinocyte derived chemokine , MCP 1 , and MIP 2 are secreted ; and GR1+ / CD45+ PMN are recruited into lungs 3 days postinfection . ^^^ Six days postinfection , however , MyD 88 deficient mice were able to produce TNF , IFN gamma , keratinocyte derived chemokine , and MCP 1 in amounts similar to wild type mice , but failed to secrete IL 12p40 and MIP 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It is hypothesized that T lymphocytes play a major role in destroying the bone marrow ( BM ) stem cells of AA patients by infiltrating the BM and secreting excessive levels of anti hematopoietic cytokines , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) . ^^^ We undertook this study to assess the pathogenic significance of anti hematopoietic cytokines such as IFN gamma and TNF alpha in BM T cells and plasma of AA patients . ^^^ Significantly elevated levels of IFN gamma and TNF alpha were found in the BM plasma of AA patients compared to controls ( p=0 . 05 and 0 . 006 , respectively ) . ^^^ Intracellular IFN gamma and not TNF alpha in BM CD3+ T cells of AA patients was significantly higher compared to controls ( p=0 . 04 and p=0 . 2 , respectively ) . ^^^ We thus conclude that increased production of both IFN gamma and TNF alpha in the BM may contribute to disease pathogenesis in AA and ATG therapy may induce hematological remission by suppressing the elevated levels of IFN gamma and TNF alpha in AA BM . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Consistent with these results , TEWL was suppressed by TNF alpha and IFN gamma , and these effects were also inhibited by IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , the systemic effect on serum cytokine levels showed that HDC only moderately lowered IL 2 induced IFN gamma , IL 6 , IL 10 , IL 18 and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Finally , we determined the susceptibility to proinflammatory cytokine ( PTh 1 ) induced apoptosis by incubating HGF treated and untreated ARIP cells with a cocktail of interleukin 1 beta ( IL 1beta ) , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The T helper response to HIV , HCV and cytomegalovirus ( CMV ) as a positive viral control was dominated by type 1 cytokines ( interleukin [ IL ] 2 , interferon [ IFN ] gamma and tumor necrosis factor [ TNF ] alpha ) , with IFN gamma as the most abundantly secreted . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , intragraft mRNA expression of CD 3 , IFN gamma , TNF alpha , IL 10 and IL 4 was analyzed using real time RT PCR analysis at day 7 after transplantation before rejection occurred in additional control animals . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Genotyping for IFN gamma ( Th 1 cytokine ) , IL 6 , IL 10 ( Th 2 cytokines ) , TNF alpha and TGF beta 1 was undertaken for 33 patients with patch stage mycosis fungoides and the results were compared with those in a control group . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine mRNA [ interleukin ( IL ) 1beta , IL 2 , IL 4 , IL 6 , IL 10 , interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) ] expression was measured by quantitative reverse transcriptase polymerase chain reaction in biopsies and PBMNC , and correlated to endoscopic findings , clinical activity and outcome after 6 months GC therapy . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cultured cells from 14 human subjects were stimulated with interleukin 1 beta ( IL 1beta ) , IL 2 , IL 6 , IL 8 , IL 12 , tumor necrosis factor alpha ( TNF alpha ) , gamma interferon ( IFN gamma ) or Escherichia coli lipopolysaccharide ( LPS ) and analyzed by reverse transcription ( RT ) PCR . ^^^ IFN gamma was a primary inducer for HBD 1 and HBD 3 , while IL 1beta and TNF alpha were primary inducers for HBD 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
ATC armed with 9184Bi statistically augmented cytotoxicity directed at PC 3 and increased IFN gamma , TNF alpha , and GM CSF secretion as well as induced IFN gamma EliSpots above that seen for 9187Bi , 9189Bi , ATC alone or ATC armed with an irrelevant BiAb . 9184Bi armed ATC mediated significant cytotoxicity against LNCaP and DU 145 cells as well . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Accordingly , a significant reduction of IFN gamma , TNF alpha , and TGF beta 1 mRNA in kidneys from animals that received Treg injection was observed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment with IFN gamma before lipopolysaccharide ( LPS ) stimulation further increased tumour necrosis factor alpha ( TNF alpha ) production as compared to TNF alpha production with LPS stimulation alone ; the rate of increase in TNF alpha production was the same in both groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The change of serum level of the cytokines IL 2 , IFN gamma , TNF alpha , IL 4 , IL 5 and IL 10 was determined by ELISA during the infection which lasted for 260 d . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In functional analyses of the MRL and C3H Opn alleles using synthetic osteopontin ( OPN ) made with a new method `` cell free system ' ' with wheat germ ribosomes , the MRL OPN induced higher expression and production of immunoglobulins as well as cytokines including TNF alpha , IL 1beta and IFN gamma in splenocytes and / or macrophages than that of the C3H allele . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These NKT cells rapidly produce high levels of interleukin ( IL ) 2 , IFN gamma , TNF alpha , and IL 4 upon stimulation through their TCR . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Instillation of rfh SP D to sensitized mice 6 h after AC ( therapeutic ) , but not 24 h before AC ( preventive ) , markedly reduced infiltration of eosinophils , and also reduced levels of IL 4 , IL 5 , eotaxin , and TNF alpha but elevated levels of IFN gamma in the BALF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This increase in cell number was associated with an increase in CD 8 T cell function , as defined by specific IFN gamma and TNF alpha production , and protection from tumor challenge . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Polymorphism of cytokine genes ( TGF beta 1 , IFN gamma , IL 6 , IL 10 , and TNF alpha ) in patients with chronic pancreatitis . ^^^ The polymorphisms studied were TNF alpha ( 308G / A ) , TGF beta 1 ( codon 10C / T , codon 25C / G ) , IL 10 ( 1082A / G ; 819T / C ; 592A / C ) , IL 6 ( 174G / C ) , and IFN gamma ( +874T / A ) . ^^^ No statistically significant differences were found for the allele and genotypic frequencies on the genes that code TNF alpha , IFN gamma , IL 10 , and TGF beta 1 codon 25 , and IL 6 between the control and case groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The aim of this study was to assess the relationship between polymorphisms involving five cytokine genes ( TNF alpha , TGF beta , IL 10 , IL 6 , and IFN gamma ) , and IBD susceptibility and disease phenotype . ^^^ The specific gene polymorphisms that were probed for included : 1082 ( G / A ) , 819 ( T / C ) , and 592 ( A / C ) in the IL 10 promoter , 308 ( G / A ) in the TNF alpha promoter , codon 10 ( T / C ) , and codon 25 ( G / C ) of the TGF beta signal sequence , +874 ( T / A ) of intron 1 of IFN gamma , and 174 ( C / G ) in the IL 6 promoter . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Thus , exposure to lower doses leads to suppression of the T ( H ) 1 cytokine IFN gamma and the proinflammatory cytokines TNF alpha and IL 1beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro it was shown that absence of FcgammaRIIB under tolerogenic conditions led to increased IgG induced release of inflammatory cytokines such as MCP 1 , TNF alpha , and IL 6 by bone marrow derived DC , and increased their expression of costimulatory molecules , resulting in an altered immunogenic T cell response associated with increased IL 2 and IFN gamma secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha and IL 1beta , which act synergistically with IFN gamma to induce IP 10 , were also up regulated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Other parameters of CD 8 ( + ) T cell function , including IL 2 , IFN gamma , TNF alpha , and granzyme production , were unaltered . ^^^ IFN gamma , TNF alpha , and granzyme production were unaltered . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CD 8 ( + ) T cell lines generated to minigene encoded CTL epitopes secreted IFN gamma and TNF alpha and exhibited CTL activity upon recognition of Cpn infected macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CD 40 stimulated macrophages acquired anti T . gondii activity that was not inhibited by a neutralizing anti IFN gamma monoclonal antibody but was ablated by the neutralization of tumor necrosis factor alpha ( TNF alpha ) . ^^^ Moreover , while the induction of anti T . gondii activity in response to CD 40 stimulation was unimpaired in macrophages from IFN gamma ( / ) mice , macrophages from TNF receptor 1 / 2 ( / ) mice failed to respond to CD 40 engagement . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Autoantibodies and serum level of cytokines ( IL 10 , TNF alpha , IFN gamma , IL 4 , and IL 12p70 ) were determined by enzyme linked immunosorbent assay . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although no difference in the counts of lymphocytes , total T cells , helper and cytotoxic T cells was observed , the counts of intracellular cytokine producing helper T cells in IFN gamma , TNF alpha or GM CSF were significantly higher in active cases than in quiescent cases . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There was a direct correlation between plasma IL 6 , TNF alpha and IFN gamma levels and the post treatment clinical scores of patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Among the strongly upregulated genes in the specimens obtained from liver transplants during CMV infection were IFN gamma , caspases 1 and 3 , granzymes A and B , TGF beta receptors 2 and 3 , IL 10 receptor alpha , VCAM 1 , TNF receptor , IL 4 , TNF alpha , IL 10 , IL 2 receptor beta , IL 1beta , PDGF receptor beta , vascular adhesion protein 1 , TGF beta 2 , and ICAM 1 . ^^^ Upregulated genes common for CMV and alloresponse were granzyme A and B , E selection , IFN gamma , VCAM 1 , VLA 4 , TNF , caspases 1 , 3 , and 8 , and PDGF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found the expression of pro inflammatory and Th 1 type cytokines including TNF alpha , IFN gamma and IL 12 in periodontal tissues after infection with A . actinomycetemcomitans , from the early stages after infection and throughout the course of the disease . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : HCKs grown in vitro were stimulated with IL 1alpha , TNF alpha , or IFN gamma . ^^^ RESULTS : HCKs incubated with TNF alpha , IL 1alpha , or IFN gamma resulted in a > 2000 fold increase in IP 10 protein secretion by 36 hours after stimulation . ^^^ In contrast , stimulation with TNF alpha , IL 1alpha , or IFN gamma induced levels of MIG and 1 TAC that were not significantly greater than constitutive levels . ^^^ Treatment of HCKs with IFN gamma activated STAT 1 and , in combination with either TNF alpha or IL 1alpha , enhanced MIG and 1 TAC synthesis > 20 fold . ^^^ CONCLUSIONS : IP 10 synthesis is induced in HCKs by IL 1alpha , TNF alpha , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The experiments measured the effects of AmB AG on ( 1 ) release of tumor necrosis factor alpha ( TNF alpha ) , nitric oxide ( NO ) , and interferon gamma ( IFN gamma ) from phagocytic cells and ( 2 ) cell mediated immune responses . ^^^ AmB AG increased TNF alpha release from mouse peritoneal macrophages and human monocytes but had no effect on IFN gamma and NO release . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : We found that interferon gamma ( IFN gamma ) and tumor necrosis factor ( TNF ) alpha mRNA expression peaked during the active phase of uveitis , whereas interleukin ( IL ) 10 mRNA increased during the disease resolution . ^^^ CONCLUSIONS : The inhibitory effects of PDTC are mainly resulted from inhibiting the expression of proinflammatory cytokines , TNF alpha and IFN gamma but augmenting anti inflammatory cytokines , IL 10 expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In mice with heavy infections , TNF alpha and IFN gamma protein concentrations were significantly higher in pulmonary lavage fluid from knockout mice . ^^^ There was a significant positive correlation between TNF alpha and IFN gamma concentrations and the level of infection in knockout mice , but not in WT mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The SARS CoV infected DCs showed low expression of antiviral cytokines ( interferon alpha [ IFN alpha ] , IFN beta , IFN gamma , and interleukin 12p40 [ IL 12p40 ] ) , moderate up regulation of proinflammatory cytokines ( tumor necrosis factor alpha [ TNF alpha ] and IL 6 ) but significant up regulation of inflammatory chemokines ( macrophage inflammatory protein 1alpha [ MIP 1alpha ] , regulated on activation normal T cell expressed and secreted [ RANTES ] ) , interferon inducible protein of 10 kDa [ IP 10 ] , and monocyte chemoattractant protein 1 [ MCP 1 ] ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The analysis for immune functions included : proliferation of splenocyte and bone marrow cells , IL 2 and IFN gamma release by splenocytes , and TNF alpha and IL 10 release by splenic , peritoneal , liver ( Kupffer cell ) , and bone marrow macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In mouse splenocyte cultures containing about 87 % lymphocytes we show that concanavalin A ( ConA , 5 microg / ml ) stimulated cells increase the expression of TNF alpha , IL 2 and IFN gamma ( ELISA ) significantly . ^^^ In our experiments , however , ryanodine has no effect on ConA stimulated IL 2 and IFN gamma expression and only suppresses TNF alpha expression by 20 % . ^^^ Furthermore , ryanodine does not prevent the inhibitory effect of caffeine on TNF alpha , IL 2 and IFN gamma expression in stimulated effector cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Analysis of cytokines in suppressed MLR cultures demonstrated up regulation of IFN gamma and IL 10 , and down regulation of TNF alpha production relative to control cultures . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here , we evaluated innate and adaptive immune system cytokine responses induced by HPV 16 L 1 VLP in whole blood ( WB ) cultures from individuals receiving the vaccine ( n=20 ) or placebo ( n=4 ) before and after vaccination . 11 cytokines were measured : IL 1beta , IL 2 , IL 4 , IL 5 , IL 6 , IL 8 , IL 10 , IL 12 , IFN gamma , TNF alpha , and GM CSF using multiplex bead arrays . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The CSF T cell clones produced IFN gamma ; some also produced TNF alpha , IL 10 and IL 5 . 5 ( H ) and 5 ( L ) on the monoclonal IgG derived from CSF B cells expressed amino acid replacements due to somatic mutations . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A predominant H . pylori specific Th 1 response , characterized by high IFN gamma , TNF alpha , and IL 12 production associates with peptic ulcer , whereas combined secretion of both Th 1 and Th 2 cytokines are present in uncomplicated gastritis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neutrophils from resistant C3H / HeJ but not from susceptible BALB / c mice expressed mRNAs for IL 12p40 , IFN gamma , TNF alpha and monokine induced by IFN gamma ( MIG ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A screening of various factors ( histamine , IFN gamma , IL 1beta , IL 2 , IL 3 , IL 4 , IL 5 , IL 8 , IL 12 , and TNF alpha ) identified IFN gamma as a potent factor capable of reducing epithelial barrier properties and enhancing transepithelial allergen penetration . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The percentages of both CD 4 ( + ) and CD 8 ( + ) cells producing type 1 ( IL 2 , IFN gamma or TNF alpha ) and type 2 ( IL 4 ) were significantly lower in patients with breast cancer compared to healthy controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The aim of the present study was to examine mRNA expression levels of Th 1 ( TNF alpha , IFN gamma , and IL 12p40 ) and Th 2 ( IL 6 and IL 10 ) cytokines for any association with clinicopathological characteristics of epithelial ovarian cancer . mRNA was isolated , and cDNA prepared from 40 samples of epithelial ovarian cancers . ^^^ Tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) expression levels were significantly higher in serous adenocarcinoma than in non serous adenocarcinoma ( p < 0 . 05 ) , but with no difference between individual cytokine mRNA expression levels and clinical stage or histological grade . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
As a first step toward exploring this , we studied in murine back skin ( C57BL / 6 ) whether intradermally injected interleukin 1beta ( IL 1beta ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) altered the cutaneous immunoreactivity patterns of nerve growth factor ( NGF ) , brain derived neurotrophic factor ( BDNF ) , neurotrophin 3 ( NT 3 ) , neurotrophin 4 ( NT 4 ) , Trk A , Trk B , Trk C and p75NTR and their receptors ( TrkA , TrkB , TrkC , p75NTR ) on the protein level in situ . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Conjunctival epithelial cells isolated from cadaveric tissues were stimulated with interferon gamma ( IFN gamma ) or a commercial S aureus cell wall extract ( Staphylococcus aureus CWE ) ( with or without anti TLR 2 blocking antibody or PAFR antagonist ) and were analyzed for tumor necrosis factor alpha ( TNF alpha ) and interleukin 8 ( IL 8 ) release ; surface expression of TLR 2 , intercellular adhesion molecule 1 , HLA , and CD 14 ; and TLR 2 messenger RNA expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine production ( IFN gamma , TNF alpha , IL 6 , IL 10 ) by whole blood cultures , circulating cytokines and acute phase proteins were analysed before and 2 days after vaccination . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines assayed include gamma interferon ( IFN gamma ) , tumor necrosis factor alpha , interleukin ( IL ) 2 , IL 4 , and IL 6 . beta Actin was used as the housekeeping gene . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found that AChR transcripts are up regulated by IFN gamma , and even more so by IFN gamma and TNF alpha , as assessed by real time RT PCR , with the alpha AChR subunit being the most sensitive to this regulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The majority of IFN gamma and TNF alpha producing lymphocytes were CD28+ in both healthy blood donors and sarcoid subjects . ^^^ These cells may contribute to the inflammatory response ; however , they are not major contributors of IFN gamma or TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We studied dose requirements for LPS , IFN gamma , and cytokines TNF alpha and IL 10 to produce nitric oxide ( NO ) in peritoneal macrophages ( Mphi ) from these animals . ^^^ The high output NO biosynthesis was dose dependently stimulated by combined administration of varying concentrations of IFN gamma ( 50 5000 pg / mL ) and LPS ( approximately 1 ng / mL ) or to a lesser extent by IFN gamma plus TNF alpha or TNF alpha / IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha , IL 10 , IL 1beta and IFN gamma were determined in amniotic fluids by ELISA , the expression of cytokines and some other inflammatory markers was determined by immunohistochemistry . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
About 90 % of T lymphoblast migration to IFN gamma , IFN gamma plus TNF alpha , polyinosinic polycytidylic acid , lipopolysaccharide , and delayed type hypersensitivity ( DTH ) induced inflammation was inhibited . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Oral treatment with EE decreased proliferation and secretion of pro inflammatory factors , TNF alpha IFN gamma , MCP 1 and IL 6 by bCII peptide specific T cells , production of bCII specific IgG2a antibodies , and mRNA for cytokines , chemokines and chemokine receptors in joint tissue . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
One week after infection , there was a significant increase in liver CD 4 ( + ) T cells producing IFN gamma and in macrophages producing TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Vitality / proliferation of PBMC was tested using the MTT assay and their functional reactivity using cytokine ELISA for interferon gamma ( IFN gamma ) , interleukin 4 ( IL 4 ) , IL 13 , and tumor necrosis factor alpha ( TNF alpha ) . ^^^ The secretion of IFN gamma , IL 4 , and IL 13 was inhibited at concentrations of 72 . 5 g / m3 and above , whereas the TNF alpha production was increased . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum leptin levels and the concentrations of IFN gamma , TNF alpha , IL 12 and IL 4 cytokines were measured by ELISA before the start of the treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The combination of IFNbeta 1b with AZA resulted in a significant negative effect on the production of IL 2 ( P=0 . 006 ) , whereas TNF alpha ( P=0 . 02 ) , IFN gamma ( P=0 . 03 ) , IL 4 ( P=0 . 2 ) , and IL 10 ( P=0 . 3 ) were not statistically impacted . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Local production of TNF , IL 1beta , IFN gamma , CCL 3 ( MIP 1alpha ) , and CCL 4 ( MIP 1beta ) was reduced in IL 18 / mice , demonstrating a local proinflammatory role for IL 18 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Polymorphism in the genes of alpha and beta tumor necrosis factors ( TNF alpha and TNF beta ) and gamma interferon ( IFN gamma ) among Iranian women with breast cancer . ^^^ The association of the polymorphism in the genes of TNF alpha , TNF beta and IFN gamma with breast cancer was investigated in the present study . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Enhanced leukocyte infiltration may be driven by increased levels of the type 1 cytokines , IL 2 and IFN gamma , and TNF alpha , observed at the site of antigen reexposure in animals that had been stressed at the time of primary immunization . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tissue culture model of renal epithelial cells expressed IL 18 mRNA constitutively and released mature IL 18 in response to TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have demonstrated increased production of Type 1 cytokines IL 2 , IFN gamma and TNF alpha as well as IL 5 , IL 6 and IL 8 by peripheral blood mononuclear cells in RAU . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
By mixing commercially available reagents and newly developed ones , a combination has been created that allows for the detection of 20 NHP chemokines and cytokines in a single sample , including G CSF , GM CSF , IFN gamma , IL 1beta , IL 1Ra , IL 2 , IL 4 , IL 5 , IL 6 , IL 8 , IL 10 , IL 12 ( p 40 ) , IL 17 , IL 18 , MCP 1 , MIP 1alpha , MIP 1beta , RANTES , TNF alpha , and TNF beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In general , the protein levels in the SM for IL 1beta , IL 6 , TNFalpha , IFN gamma , IL 4 , and IL 10 ( increase from 4 fold to 15 fold ) matched the course of mRNA expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There were also abnormally high levels of the proinflammatory cytokines interferon gamma ( IFN gamma ) , tumor necrosis factor ( TNF ) , and interleukin 12 ( IL 12 ) , and activated T cells circulating in these mice . ^^^ Additionally , Socs 1 ( null ) macrophages produced excessive amounts of IL 12 and TNF in response to other cytokines , including IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Thus , the maturation of DCs induced by CD11c T cells through TNF alpha production appears to result in the efficient expansion and activation of Valpha24+ NKT cells to produce IFN gamma preferentially . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cellular immune responses were evaluated by measuring the proliferative responses and cytokine production [ interleukin ( IL ) 4 , IL 5 , IL 6 , IL 13 , IFN gamma and tumor necrosis factor ( TNF ) alpha ] of splenocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , production of granuloma inducing cytokines , TNF alpha and IFN gamma , was delayed and chemotactic activity for macrophages was suppressed in the liver of mutant mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Activated T cells expressed high levels of CD 25 , CD 54 , CD 137 , and CD 154 , and produced IFN gamma , TNF alpha , and GM CSF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study we show that rocaglamides are potent immunosuppressive phytochemicals that suppress IFN gamma , TNF alpha , IL 2 , and IL 4 production in peripheral blood T cells at nanomolar concentrations . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
KMP 11 DNA vaccinated hamsters were protected by a surge in IFN gamma , TNF alpha , and IL 12 levels along with extreme down regulation of IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
They inhibited production of IFN gamma , TNF alpha , and chemokines in autologous and HLA class 1 matched heterologous synovitis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) were elevated at 4 h in the plasma . ^^^ The lethal effect was suppressed in TNF alpha KO mice , TNF receptor 1 KO mice , and lpr / lpr ( Fas deficient ) mice , whereas it was not in IFN gamma KO mice . ^^^ Tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) were elevated at 4 h in the plasma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Reverse transcription polymerase chain reaction ( PCR ) was performed and cytokine mRNA expression for tumor necrosis factor alpha ( TNFalpha ) , interleukin 1beta ( IL 1beta ) , interleukin 10 ( IL 10 ) and interferon gamma ( IFN gamma ) determined using real time PCR . ^^^ A significant effect of LPS stimulation over time was seen on TNFalpha , IL 1beta , IL 10 and IFN gamma production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Synergistic activation of JNK / SAPK induced by TNF alpha and IFN gamma : apoptosis of pancreatic beta cells via the p 53 and ROS pathway . ^^^ IFN gamma and TNF alpha are major proinflammatory cytokines implicated in islet beta cell destruction , which results in type 1 diabetes ; however , the underlying mechanism is not clear . ^^^ Using pancreatic beta cell line MIN6N8 cells , co treatment with TNF alpha and IFN gamma , but neither cytokine alone , synergistically induced apoptosis , correlated with the activation of the JNK / SAPK , which resulted in the production of reactive oxidative species ( ROS ) and loss of mitochondrial transmembrane potential ( delta psi m ) . ^^^ Additionally , cells transfected with wild type JNK 1 became more susceptible to apoptosis induced by TNF alpha / IFN gamma through ROS production and loss of delta psi m , while cascading apoptotic events were prevented in dominant negative JNK 1 transfected or JNK inhibitor SP 600125 treated cells . ^^^ As the antioxidant , N acetyl cysteine , failed to completely suppress apoptosis induced by TNF alpha / IFN gamma , an additional pathway was considered to be involved . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
For the functional doses chosen , cytokines ( IL 2 , IL 4 , IL 6 , IL 10 , IL 12 , TNF alpha and IFN gamma ) were determined . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
HPCs from marrows of mice expressing an SDF 1 / CXCL12 transgene were insensitive to inhibition by SDF / CXCL12 sensitive myelosuppressive chemokines , but not to SDF / CCL12 insensitive cytokines ( TNF alpha , IFN gamma , TGF beta , H Ferritin , or LF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
BAL concentrations of cytokines TNF alpha , IFN gamma , and chemokines MIG , RANTES and MIP 1alpha were significantly elevated in both strains of RSV infected mice compared with their respective controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The analysis of typical T ( H ) 1 / T ( H ) 2 cytokines ( IFN gamma , TNF alpha , IL 12p70 , IL 2 , IL 4 , IL 5 , and IL 10 ) revealed a strong T ( H ) 1 response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Primary astrocytic cultures of newborn rats were cocultured with either 5 % ( M 5 ) or 30 % ( M 30 ) microglial cells and were incubated with the following proinflammatory cytokines : tumor necrosis factor alpha ( TNF alpha ) , interleukin 1beta ( IL 1beta ) , interleukin 6 ( IL 6 ) , interferon gamma ( IFN gamma ) , and the antiinflammatory cytokines transforming growth factor beta 1 ( TGF beta 1 ) and IFN beta . ^^^ Finally IFN beta , added to M 5 cocultures was able to prevent the effects of the proinflammatory cytokines TNF alpha , IL 1beta , and IFN gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of healthy mice or mice bearing sarsoma 180 with oral Ganopoly over 20 100 mg / kg for 7 day significantly increased the expression of both TNF alpha and IFN gamma ( at both mRNA and protein levels ) in splenocytes in a dose dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Anti TNF Ab reduced Fas ligand ( Fas L ) expression of NKT cells while it completely inhibited organ injuries induced by alpha GalCer and thereby reduced the mortality of old mice , whereas it did not affect the IFN gamma production from NKT cells , the antitumor immunity in the liver nor the mouse survival after EL 4 injection . ^^^ CONCLUSIONS : NKT cells activated by alpha galactosylceramide participated in either antitumor immunity or hepatic injury using IFN gamma and TNF / Fas L , respectively . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The stimulating agents LPS , TNF alpha , IL 1beta , and IL 4 were all able to synergize with IFN gamma , stimulating large concentrations of NO compared to normal mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines , such as IL 10 , GMCSF , IFN gamma , and TNF alpha were also detected in the supernatants of the cultured human cells from the chimeric mice , when they were stimulated with allogeneic cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We report our CD8+ NK / T cell line SRIK NKL , derived from leukemic phase of acute lymphoblastic lymphoma , expresses mRNA and protein for 8 / 10 TLR ' s , associated proteins for signaling , defensins , cathelicidin / LL 37 , and responds to live bacteria by cell proliferation and increased IFN gamma , TNF alpha , TNF beta , and MIP 1alpha production . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Intracellular IL 2 , TNF alpha , IFN gamma expression in CGP 41251 treated T cells stimulated by lectin was measured by flow cytometry . ^^^ CGP 41251 inhibited TNF alpha production by T cells with an IC ( 50 ) of 0 . 5 microM and did not significantly inhibit the production of IL 2 or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The compound K also significantly reduced the levels of mRNA of cyclooxygenase ( COX ) 2 , IL 1beta , TNF alpha , IFN gamma and IL 4 increased in oxazolone applied mouse ears . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : We measured the concentrations of interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 2 ( IL 2 ) , IL 4 , IL 6 , and IL 10 in CSF during the acute stage in 15 children with mumps meningitis ( MM ) , and 34 with echovirus type 30 meningitis ( EM ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Capacity of unstimulated and mitogen stimulated peripheral blood mononuclear cells ( PBMCs ) to secrete cytokines ( interleukin ( IL ) 2 , interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , IL 4 , IL 10 and IL 6 ) was estimated for 15 HIV TB coinfected patients , 22 HIV seropositives without TB , 32 HIV negative TB patients , and 36 healthy subjects . ^^^ Dually infected patients had suppression of both Th 1 and Th 2 cytokine secretion as evidenced by significantly lower production of IL 2 , IFN gamma and TNF alpha as well as IL 4 and IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The failure to recruit Gr 1+ monocytes resulted in greatly enhanced mortality despite the induction of normal Th 1 cell responses leading to high levels of IL 12 , TNF alpha , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The samples were evaluated for 13 cytokines ( IL 1B , IL 2 , IL 4 , IL 5 , IL 6 , IL 7 , IL 8 , IL 10 , IL 12 , IL 13 , IFN gamma , GM CSF , and TNF alpha ) using the Luminex bead array ( LINCOplex ) multianalyte detection system that permits simultaneous detection of multiple cytokines from a single sample . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
AIM : To investigate whether there was a relationship between the liver functions and fibrosis scores of hepatitis B patients and their TNF alpha , IFN gamma , IL 4 , and TGF beta ( 1 ) serum levels based on the studies of liver biopsies . ^^^ RESULTS : In comparison of cytokine levels between CHB patients and control group , TNF alpha , IL 4 , and TGF beta ( 1 ) levels of the patients were higher in CHB patients than in the controls , while IFN gamma level was lower in the patients than in the controls . ^^^ There were significant differences between the groups in TNF alpha , IL 4 , TGF beta ( 1 ) , and IFN gamma ( P < 0 . 005 , 0 . 03 , 0 . 002 , 0 . 0001 , respectively ) . ^^^ There was a negative correlation between TGF beta ( 1 ) and IL 4 and IFN gamma ( P < 0 . 05 ) , TNF alpha and the other cytokines and IFN gamma and IL 4 were not correlated ( P > 0 . 05 ) . ^^^ Liver necroinflammatory activity and fibrosis and TNF alpha , IL 4 , and IFN gamma were not correlated ( P > 0 . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RECENT FINDINGS : A decrease in the secretion of pro inflammatory cytokines , IFN gamma , TNF alpha and IL 12 , and interference with bacterial adherence to the epithelium has been demonstrated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Leptin produced by adipocytes , tumour necrosis factor ( TNF ) alpha and interleukin ( IL ) 1beta by macrophages , and IFN gamma and IL 4 by lymphocytes were quantified by enzyme linked immunosorbent assay ( ELISA ) tests . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RU 486 and nadolol treatment also tended to decrease IL 10 , IFN gamma , TNFalpha ( nadolol only ) , and IL 1beta ( RU 486 only ) in both exercise and control mice , suggesting that stress hormones may be necessary during infection for optimal responsiveness . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , both in vitro and in vivo microdialysis sampling of different cytokines ( tumor necrosis factor alpha [ TNF alpha ] , interferon gamma [ IFN gamma ] , interleukin 6 [ IL 6 ] , IL 12p70 , and macrophage chemoattractant protein 1 [ MCP 1 ] ) was performed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
At the onset of radiation fibrosis in C57BL / 6J mice ( 5 6 months ) , irradiated lungs had increased levels of IL 1alpha / beta and IFN gamma mRNA expression , but the TNF alpha response was , notably , still muted . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The etiology of chronic fatigue syndrome ( CFS ) has been both obscure and highly contentious , leading to substantial barriers to both clear diagnosis and effective treatment . 1 propose here a novel hypothesis of CFS in which either viral or bacterial infection induces one or more cytokines , IL 1beta IL 6 , TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the presence of IL 18 , simvastatin suppressed the expression of ICAM 1 and CD 40 as well as the production of IL 12 , TNF alpha and IFN gamma in PBMC , contributing to the anti inflammatory effect of simvastatin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found that CD 14 ( + ) monocytes infected by either HIV 1 ( 213 ) ( X 4 ) or HIV 1 ( BaL ) ( R 5 ) produced IL 10 , IL 6 , tumor necrosis factor alpha ( TNF alpha ) , and to a lesser extent , IFN gamma . ^^^ Cross linking CD 4 molecules ( XLCD 4 ) with anti CD 4 mAbs and goat anti mouse IgG ( GAM ) resulted in high levels of IL 6 , TNF alpha and IFN gamma but no IL 10 production by CD 14 ( + ) monocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Accumax was tested in 16 different ELISPOT assays , including human interleukin ( IL ) 2 , IL 4 , IL 5 , IL 6 , IL 8 , IL 13 , IL 1beta , interferon ( IFN ) gamma , and tumor necrosis factor ( TNF ) alpha ; mouse IL 4 , IL 6 , IFN gamma , and TNF alpha ; rat IL 2 and IFN gamma ; and canine IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The proportion of CD4+ T lymphocytes expressing IFN gamma did not differ between the groups , whereas a trend towards increased proportions of TNF alpha expression in CD4+ T cells was observed in the TB compared to the HIV group , while intermediate values were observed in co infected patients . ^^^ Detection of IFN gamma and TNF alpha in CD8+ T lymphocytes was higher in TB than in HIV individuals . ^^^ Co infected patients presented intermediate values for IFN gamma , while TNF alpha detection was similar to that in HIV mono infection . ^^^ In conclusion , the proportion of T cells expressing IFN gamma was relatively preserved in co infected patients compared to TB patients , while the percentage of T cells expressing TNF alpha was decreased , mainly in CD8+ T lymphocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NK cell induced DC activation is dependent on both tumor necrosis factor alpha ( TNF alpha ) / interferon gamma ( IFN gamma ) secretion and a cell cell contact involving NKp 30 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
On the mRNA level , the ratio of TNFalpha / IL 10 , IFN gamma / IL 10 , and IL 12p40 / IL 10 decreased after addition of PC ( P < 0 . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mitogenic stimulation with PMA and ionomycin induced much higher frequencies of IFN gamma and TNF alpha expressing CD4+ , CD8+ as well as TCRgammadelta+ cells in adults , while differences for interleukin ( IL ) 2 expression were restricted to CD4+ and CD8+ T cells . ^^^ Impressive increases in the capacity to produce P . falciparum specific and non specific IFN gamma and TNF alpha appear to be the main cellular correlates of naturally acquired immunity in Central Africa . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor ( TNF ) alpha also stimulated IP 10 mRNA expression , but this was much weaker than that induced by IFN gamma . ^^^ The effects of IFN gamma and TNF alpha were detected in a dose and time dependent manner . ^^^ Trichostatin A , a specific inhibitor of histone deacetylase , also blocked the IFN gamma and TNF alpha induced IP 10 mRNA expression , but the effects of trichostatin A were weaker than those of butyrate . ^^^ Tumor necrosis factor ( TNF ) alpha also stimulated IP 10 mRNA expression , but this was much weaker than that induced by IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The object of this study was to detect effects of 10 ( 4 ) , 10 ( 5 ) , and 10 ( 6 ) M Cd sulphate , Zn sulphate , and sodium selenite , and their combinations on human peripheral blood mononuclear cell ( PBMC ) proliferation and IFN gamma and TNF alpha production . ^^^ Moreover , 10 ( 4 ) and 10 ( 5 ) M Zn and 10 ( 5 ) M Se strongly upregulated IFN gamma ( a Th 1 cytokine ) release , even in presence of 10 ( 5 ) M Cd , and reduced the inhibitory effects of Cd on PBMC proliferation and TNF alpha release . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
For that purpose , we measured the circadian changes in the expression of clock genes ( Per 1 , Per 2 , Bmal 1 , Clock ) , Dbp ( a clock controlled output gene ) , CREB ( involved in clock signaling ) , cytolytic factors ( granzyme B and perforin ) , and cytokines ( IFN gamma and TNF alpha ) in NK cells enriched from the rat spleen . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma spots may have only 25 microm diameters , and TNF alpha spots just 15 microm . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Nasal samples from cats positive for FHV 1 mRNA displayed significantly increased transcription of IL 6 , IL 10 , IL 12p40 , IL 18 , IFN gamma , TNF alpha , and RANTES ( P < 0 . 05 ) in comparison to samples from cats negative for FHV 1 mRNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunocytochemistry revealed macrophages / activated microglia expressing IDO during EAE and in vitro experiments confirmed IDO induction in microglia upon IFN gamma treatment with synergistic effects of TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
All available paired pretreatment and posttreatment plasma samples ( n = 132 ) were analyzed for concentrations of IL 2 , IL 4 , IL 6 , IL 10 , TNF alpha , IFN gamma , soluble intercellular adhesion molecule 1 , soluble E selectin , TGF beta 1 , TGF beta 2 , and C reactive protein . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mouse peritoneal macrophage cultures were used to understand ex vivo effects of RP 1 on LPS generated nitric oxide ( NO ) , secretion of IFN gamma , IL 6 and TNF alpha . ^^^ Ex vivo study revealed that RP 1 ( 50 microg / ml ) treatment to peritoneal macrophages inhibited LPS ( 5 microg / ml ) induced nitrite generation to 37 % , IFN gamma secretion to 5 % , IL 6 secretion to 50 % and TNF alpha secretion to 50 % of LPS treated control values . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Further synergistic effects with IFN gamma in the induction of apoptosis were also observed with agonistic antitumour necrosis factor ( TNF ) related apoptosis inducing ligand ( TRAIL ) receptor 2 antibody , soluble TRAIL and TNF alpha . ^^^ Beside its apoptosis inducing capacity in HaCaT cells , rIFN gamma also induced autocrine IFN gamma production , and combined treatment with IFN gamma and TNF alpha induced autocrine TNF alpha production . ^^^ CONCLUSIONS : Taken together , our data suggest a central role for IFN gamma in HaCaT keratinocyte apoptosis but also show the importance of co acting mediators such as TNF alpha , TRAIL and FasL , which potentiate the effect of paracrine and autocrine IFN gamma and TNF alpha release . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Flow cytometric analysis demonstrated the constitutive expression of SLA class 1 and CD 86 , whereas the expression of E selectin and SLA class 2 was only induced after stimulation with human TNF alpha and pig IFN gamma , respectively . ^^^ On the other hand , no CD 80 expression was detected in the primary cells or cell lines in the presence or absence of either human TNF alpha or pig IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Previous studies have suggested a major role for interferon gamma ( IFN gamma ) and TNF in this process . ^^^ Moreover , the levels of transgene expression following in vivo gene delivery were markedly increased in mice lacking functional type 1 IFN receptor genes , compared to wild type mice or mice lacking IFN gamma or TNF receptors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
No differences were found in unstimulated levels of TNF alpha , IFN gamma , or TGF beta 1 between infertile patients and controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human scFv G 8 ( POS ) T lymphocytes comprising the gamma + CD 28 vs the gamma signaling element alone produced substantially more IL 2 , TNF alpha , and IFN gamma in response to HLA A1 / MAGE A 1 ( POS ) melanoma cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , SAG 1 ( c ) mutants produced reduced brain cyst loads early in chronic infection , but these substantially increased over time accompanying a hyperproduction of IFN gamma , TNF alpha , and IL 10 , and severe encephalitis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Eighty eight females with breast carcinoma ( BC ) were studied in this case control study comparing the cytokine genotypes of TNF alpha TGF beta 1 , IL 10 , IL 6 , and IFN gamma with controls . ^^^ The other cytokine genotypes studied ( IL 10 , IL 6 , IFN gamma , and TNF alpha production were not associated with breast cancer overall or relapse status . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , the ligation of IRS induces ( 1 ) production of tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) and ( 2 ) brisk cytolytic activity against cells bearing appropriate IRS counter ligands . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In healthy male subjects there is a proportionate relationship between supplementary intake of EPA and DHA ( 0 . 3 2 . 0 g as fish oil / d ) in combination with antioxidants ( vitamins A , C and E and Se ) and incorporation into plasma phospholipids and PBMC , and a non linear relationship with PBMC synthesis of TNF alpha , IL 6 and prostaglandin E 2 ( decrease ) and IFN gamma ( increase ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
At the protein level , an increased capacity of peripheral blood mononuclear cells to produce IL 2 , IL 4 , IL 6 , IL 12 , TNF alpha and IFN gamma was evident . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Recent progress in elucidating host innate and adaptive immune responses to M . tuberculosis ( Mtb ) and their impact on latent infection includes identification of TLR 2 dependent anti inflammatory responses , a MyD 88 independent , non protective Th 1 response , the formation of secondary lymphoid follicles in granulomas and the role of Th 1 responses , IFN gamma and TNF alpha in preventing re activation of infection ; IFN gamma also appears to be involved in activating latency genes in Mtb . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
At 24 h after injury , expression of proinflammatory cytokines IL 1beta , TNF alpha , IFN gamma , and inducible nitric oxide synthase was significantly reduced and that of anti inflammatory IL 10 and bFGF , TGF alpha , and Bcl 2 was highly upregulated in treated kidneys . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , cultured human TECs were exposed to proinflammatory factors interferon gamma ( IFN gamma ) and tumor necrotic factor alpha ( TNF alpha ) in presence or absence of sinomenine for 72 h , followed by analysis of surface expression of costimulatory molecules . ^^^ Flow cytometric analysis revealed that various costimulatory molecules were expressed on TECs and that they were significantly up regulated by the simulation of IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Action of prolactin , IFN gamma , TNF alpha and LPS on heme oxygenase 1 expression and VEGF release in human monocytes / macrophages . ^^^ Our goal in this study was to investigate the regulatory interaction between PRL , interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) and lipopolysaccharide ( LPS ) in the heme oxygenase 1 ( HO 1 ) expression and the vascular endothelial growth factor ( VEGF ) production in human monocytes / macrophages ( HMMs ) . ^^^ In vitro treatment of HMMs with PRL , IFN gamma , TNF alpha and LPS was found to increase both HO 1 expression and protein synthesis in a time dependent manner . ^^^ HMMs treated with PRL , IFN gamma , TNF alpha and LPS also showed an enhanced release of VEGF . ^^^ Moreover , co stimulation of PRL with LPS caused activation of HMMs functions , enhancement of HO 1 expression and induction of VEGF release , whereas addition of PRL inhibited up regulation of HO 1 or VEGF induced by IFN gamma or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our aims were to measure monocyte function ( ie , HLA DR expression and tumor necrosis factor alpha [ TNF alpha ] production as markers of immune suppression ) in patients with severe AP and to determine whether treatment of blood samples with granulocyte macrophage colony stimulating factor ( GM CSF ) and / or interferon gamma ( IFN gamma ) corrected the functional defects of monocytes in vitro . ^^^ IFN gamma increased TNF alpha production by monocytes in patients ( 683 pg / mL ; range , 186 2705 pg / mL ; P < 0 . 05 ) but not in controls ( 1658 pg / mL ; range , 765 4755 pg / mL ; P = 0 . 31 ) . ^^^ With the combination of GM CSF and IFN gamma , the TNF alpha levels of monocytes in patients ( 3185 pg / mL ; range , 545 8280 pg / mL ) and in controls ( 2800 pg / mL ; range , 1080 6860 pg / mL ) were comparable . ^^^ The GM CSF and IFN gamma treatments also increase LPS induced TNF alpha production . ^^^ By the combination of GM CSF and IFN gamma , but not by either agent alone , LPS induced TNF alpha production of monocytes was equally high in patients and in controls . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Oligodendrocytes in culture were exposed to interferon gamma ( IFN gamma ) , interleukin 1beta ( IL 1beta ) , and tumor necrosis factor alpha ( TNF alpha ) . ^^^ Toxicity was observed in a dose dependent manner for IFN gamma and TNF alpha . ^^^ The toxic concentration of IFN gamma , and TNF alpha was lower if the cells were iron loaded , but iron loading had no effect on the toxicity of IL 1beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma and TNF alpha inhibit expression of TGF beta 1 , its receptors TBETAR 1 and TBETAR 2 in the corpus luteum of PMSG / hCG treated rhesus monkey . ^^^ Injection of interferon gamma ( IFN gamma ) or tumor necrosis factor alpha ( TNF alpha ) at the functional stage of CL development significantly decreased the expression of StAR , as well as TGF beta 1 , and its receptors TbetaR 1 and TbetaR 2 . ^^^ Our results suggest that TGF beta 1 and its receptors may play an important regulatory role in maintaining CL function , and that IFN gamma or TNF alpha is capable of inhibiting their expression in the CL . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , CypA augmented the capacity of DCs to present antigens to allogenic CD8+ T cells , and also increased the secretion of Th 1 type cytokines TNF alpha and IFN gamma from the allogenic CD4+ T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Upon stimulation with tumor necrosis factor alpha ( TNF alpha ) plus interferon gamma ( IFN gamma ) , antisense transfectants showed less iNOS enzymatic activity with less nitric oxide ( NO ) when compared to the sense control cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
C3H / HeN mice produced high levels of chemokines macrophage inflammatory proteins 1 alpha ( MIP 1 alpha ) , MIP 2 , monocyte chemoattractant protein 1 ( MCP 1 ) , and cytokines gamma interferon ( IFN gamma ) , interleukin 12 ( IL 12 ) , IL 10 , and tumor necrosis factor alpha ( TNF alpha ) in response to O . tsutsugamushi infection , compared to BALB / c mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , compared with the high avidity of SSp 1 specific CTLs derived from memory T cells of recovered SARS patients , SSp 1 specific CTLs from the five exceptional healthy donors were of low avidity , as determined by their rapid tetramer dissociation kinetics and reduced cytotoxic reactivity , IFN gamma secretion , and intracellular production of IFN gamma , TNF alpha , perforin , and granzyme A . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Determinations : Survival , Bacterial Translocation , myeloperoxidase ( MPO ) , Cytokines ( TNF alpha , IL 1beta , IFN gamma ) , Oxygen Free Radical ( OFR ) SOA and detoxifying enzymes ( SOD , Superoxide Dismutase , CAT , Catalase and GPX , Glutathione Peroxidase ) , Cell Adhesion Molecules , CAMs ( ICAM 1 , VCAM 1 and PECAM 1 ) and Nuclear Transcription Factor , NFkappaB . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our data implicate inflammation in the pathogenesis of diabetic nephropathy in view of the overexpression of the proinflammatory cytokines TNF alpha and IFN gamma and the cells that secrete them in the early and late phases of the disease . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation of peritoneal macrophages with rHagB resulted in the production of the proinflammatory cytokines interleukin 12p40 ( IL 12p40 ) , gamma interferon ( IFN gamma ) , and tumor necrosis factor alpha , as well as the anti inflammatory cytokine IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
StxB 1 treated DCs exhibited an increase in tumor necrosis factor alpha and interleukin 12 ( IL 12 ) production , a stimulation of DO11 . 10 T cell proliferation , and the production of both Th 1 and Th 2 cytokines , including gamma interferon ( IFN gamma ) , IL 4 , IL 5 , IL 6 , and IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CD 4 ( + ) peritoneal exudate cells obtained 10 days postinfection predominantly coexpressed TNF alpha , IFN gamma , and IL 2 after polyclonal or Ag stimulation . ^^^ A population of cells simultaneously making TNF alpha and IFN gamma was also detected but at a lower frequency . ^^^ By following the kinetics of the response to Listeria , we found that CD 4 ( + ) lymphocytes coexpressing TNF alpha and IFN gamma dominated on day 6 postinfection and then declined . ^^^ From days 10 27 , TNF alpha ( + ) IFN gamma ( + ) IL 2 ( + ) ( triple positive ) was the most prevalent cytokine phenotype , and the frequency steadily declined . ^^^ Triple positive cells were preferentially noted in the peritoneal cavity tissue site ; spleen cells displayed a predominant population of double positive T cells ( TNF alpha ( + ) IFN gamma ( + ) ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The anti inflammatory effects of AICAR were associated with the inhibition of the Ag specific recall responses and inhibition of the Th 1 type cytokines IFN gamma and TNF alpha , whereas it induced the production of Th 2 cytokines IL 4 and IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Ingestion of mycotoxin extract increased the expression of IL 8 , IL 18 and IFN gamma mRNA compared with P . multocida infection that increased the expression of TNF alpha . ^^^ TNF alpha , IFN gamma and IL 18 mRNA expression was also increased . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This costimulatory signal in the immunological synapse leads to a marked increase ( 3 to 34 fold ) in the production of the T helper 1 and proimmflamatory cytokines IFN gamma , TNF alpha , and IL 6 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma , TNF alpha ) and type 2 ( i . e . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cell viability was quantified after incubation with different chemical reactive oxygen species ( ROS ) generators and with cytokines ( IL 1beta alone or a mixture of IL 1beta , TNF alpha and IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The kinetics of parasite proliferation and the effects of interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) in infected organotypic cultures were monitored by light microscopy , transmission electron microscopy ( TEM ) , and quantitative polymerase chain reaction ( PCR ) assay . ^^^ The cellular integrity in IFN gamma treated organotypic slice cultures was severely impaired compared with untreated and TNF alpha treated cultures . ^^^ Thus , on infection of organotypic neuronal cultures , IFN gamma and TNF alpha exhibit largely detrimental effects , which could contribute to either inhibition or acceleration of parasite proliferation during cerebral toxoplasmosis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Microglia from 9 different donors were compared , also following challenge of such microglia with the pro inflammatory cytokines TNF alpha and IFN gamma . ^^^ Yet , treatment with TNF alpha and IFN gamma led to widely different response profiles among the different cultures . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
LPS increased significantly the production of TNF alpha , IFN gamma , and IL 10 from liver ( 521 % , 123 % , 72 % : p < 0 . 01 , 0 . 04 , 0 . 04 ) , brain ( 470 % , 122 % , 280 % : p < 0 . 01 , 0 . 03 , 0 . 01 ) , peritoneal lavage ( p < 0 . 001 ) and blood ( p < 0 . 001 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The induction of tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) and interleukin 12 ( IL 12 ) leads to inhibition of IL 5 , with animal models demonstrating immune deviation away from Th 2 immune responses . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , we studied levels of the pro inflammatory IL 6 , Il 12 , IFN gamma , and TNF alpha , and of the anti inflammatory IL 5 and IL 13 in stimulated blood cell cultures from 27 AD patients and 25 controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
HSP 70 2 , IL 6 , IFN gamma , TNF alpha gene expressions within the plaques and serum levels of triglyceride , total cholesterol and LDL cholesterol were tested from patients stratified according to their B+ ( AB and BB ) and B ( AA ) genotypes . ^^^ B+ patients showed increased HSP 70 2 , IL 6 , IFN gamma , TNF alpha and dyslipidemia as compared to B carriers . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Elevated IL 12p40 levels had been detected in 58 % of the sepsis patients , whereas only a few had detectable TNF alpha , IFN gamma , or IL 12p70 levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We examined the role of LPL in modulating tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) mediated inflammatory cytokine signal transduction pathways in human aortic endothelial cells ( HAECs ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation of CD4+CD25 T cells with porcine cells resulted in secretion of IFN gamma , TNF alpha , IL 10 , IL 6 and IL 2 , and Treg cells suppressed the secretion of these cytokines , as well as the CD4+CD25 T cell cytolytic response against porcine cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
LMP 1 mediated SAP / ERK / IFN gamma signals appear to act via the TNF receptor associated factor ( TRAF ) 2 , 5 / nuclear factor kappaB ( NF kappaB ) pathway , since dominant negative TRAF2 / 5 and NF kappaB inhibitor could rescue SAP expression and downregulate IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
GRA treatment resulted in reduced levels of IL 10 and IL 4 , but increased levels of IL 12 , IFN gamma , TNF alpha , and inducible NO synthase , reflecting a switch of CD 4 ( + ) differentiation from Th 2 to Th 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We analyzed the mechanism whereby isolated islets from ALR mice resisted proinflammatory stress mediated by combined cytokines ( IL 1beta , TNF alpha , and IFN gamma ) in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Then , gammadelta T cells expressed various cytokines like XCL 1 2 , CCL 3 4 , TNF alpha , and IFN gamma in response to 2M3B1PP in a time dependent manner , while transient expressions were observed in IBA during the time period . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Endogenous levels of IL 10 inhibited the production of IL 12 , IFN gamma , TNF alpha , and IL 6 from both hematopoetic and non hematopoetic cells in the brain , as well as anti microbial activity of astrocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To investigate the mechanism of his fluctuating thyroid status , we serially assessed the serum levels of cytokines ( TNF alpha , IFN gamma , IL 1beta , IL 2 , IL 4 ) and the soluble form of ICAM 1 ( sICAM 1 ) as well as the activities of two types of TSH receptor antibodies ( TRAb ) , TSAb and TSBAb , before and after IFN therapy . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
As nasal inflammation in study cats worsened from absent ( n = 3 ) to mild ( 4 ) to moderate ( 8 ) or severe ( 10 ) , progressively and significantly increasing transcription of IL 6 , IL 10 , IL 12 p 40 , IFN gamma , TNF alpha , and the RANTES protein was detected . ^^^ CONCLUSIONS AND CLINICAL RELEVANCE : Transcription of specific cytokines and chemokines in nasal tissue of cats progressively increased with severity of histologic evidence of inflammation , and IL 6 , IL 10 , IL 12 p 40 , IFN gamma , TNF alpha , and the RANTES protein were markers of inflammation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
DESIGN AND METHODS : Serum IgG , IgM , IgA , IgE , IL 2 , IL 4 , TNF alpha , and IFN gamma levels were measured using immunoassay in 20 human cases of lindane poisoning . ^^^ RESULTS : Serum IL 2 , IL 4 , and TNF alpha levels were significantly raised with decrease in IFN gamma levels in the lindane exposed cases . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Recently , we have reported that rLcrV and rYopB inhibit the production of TNF alpha , IFN gamma , and IL 12 in murine peritoneal macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the effect of several cytokines ( GM CSF , IFN gamma , TNF alpha , IL 3 ) and other stimuli ( vaso 1 , LPS , PMA ) on MNGC formation in vitro by microglia from porcine neonatal brain . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment resulted in increased numbers of CD 4 cells infiltrating the colonic lamina propria , increased interferon gamma ( IFN gamma ) levels , and increased transcripts for tumour necrosis factor alpha ( TNF alpha ) and matrix metalloproteinase 3 ( MMP 3 ) . ^^^ Isolated lamina propria lymphocytes from mice with TNBS colitis contained increased numbers of IFN gamma and TNF alpha transcripts when stimulated with MIP 1alpha in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Th 1 cells , characterized by secretion of IFN gamma and TNF alpha , are primarily responsible for activating and regulating the development and persistence of CTL . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The dynamic effect of yiqi qingwen jiedu heji on the protein expressions of cytokine IFN gamma , TNF alpha , IL 10 and IL 6 in the lungs of mice infected by 4 FM 1 ] . ^^^ METHOD : Taking 4 FM 1 infected mice as its model and doing ELISA ( double antibody sandwichenzyme linked immunosorbent assay ) , we dynamically observed the change of cytokine TNF alpha , IL 6 , IFN gamma and IL 10 after giving Yiqi Qingwen Jiedu Heji treatment . ^^^ RESULT AND CONCLUSION : After the mice are infected by influenza virus , their protein expressions of the model group are higher than those of the control group , of which TNF alpha , IL 6 , IFN gamma reach the peak in three days . ^^^ It indicates that Yiqi Qingwen Jiedu Heji can resist the expressions of TNF alpha , IL 6 and IFN gamma pro inflammatory cytokine , increase the expression of IL 10 , and thus , alleviate inflammatory injury . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Free IL 18 but not IL 12 concentrations significantly correlated with clinical status and the biologic markers of HPS such as anemia ( P < . 001 ) , hypertriglyceridemia , and hyperferritinemia ( P < . 01 ) and also with markers of Th 1 lymphocyte or macrophage activation , such as elevated concentrations of IFN gamma and soluble IL 2 and tumor necrosis factor alpha ( TNF alpha ) receptor concentrations . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , Fumigaclavine C , given both in vivo and in vitro , showed a marked inhibition on the expression of several inflammatory cytokines , including IL 1beta , IL 2 , IL 12alpha , IFN gamma , TNF alpha as well as MMP 9 in sacral lymph node cells , colonic patch lymphocytes and colitis tissues from the TNBS colitis mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
None of IL 1ralpha , IL 1alpha , IL 1beta , IL 2 , IL 4 , IL 10 , IL 18 , TNF alpha , G CSF and IFN gamma could be detected in all culture media . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
T cells from mice infused with IL 15 following vaccination did not secrete increased levels of tumor specific TNF alpha or IFN gamma or have enhanced C6VL specific CTL activity compared to T cells from recipients of the vaccine alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , the release of TNF alpha , IFN gamma , IL 4 , and IL 10 by Con A stimulated splenocytes obtained from infected mice on days 5 , 10 , and 15 of the infection was significantly inhibited by treatment of the animals with salicylate or meloxicam . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we have concurrently assayed for IL 2 , IL 4 , IL 6 , IL 10 , TNF alpha , and IFN gamma in 24 h serum free cultures of peripheral blood mononuclear cells ( PBMC ) obtained from seventeen patients with prostate cancer ( CaP ) per cytokine bead array analysis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Single cold exposure significantly decreased IL 2 , 3 , 4 , 5 , 10 , 12 , IFN gamma production in BALB / c mice and decreased IL 2 content and increased TNF alpha level in C57Bl / 6 mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We studied local expression of insulin like growth factor 1 , insulin like growth factor receptor , epithelial growth factor , transforming growth factor beta 2 , PCNA , TNF alpha , type 1 TNF receptor , Fas , FasL , IFN gamma , IL 1beta , IL 4 , IL 6 , IL 8 , IL 10 , and IL 12 genes in intact and hyperplastic endometrium . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Excellent correlations were demonstrated for IL 1 beta , IL 4 , IL 5 , IL 6 , IL 10 , IFN gamma , and TNF alpha , in contrast to IL 12 p 70 and IL 13 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There is an absence of a classical polarisation of either T helper ( Th ) 1 or Th 2 cytokine responses , although increased expression of mRNA for interleukin ( IL ) 2 and IL 12p40 and a shift towards mucosal immunoglobulin ( Ig ) G production are consistent findings , whilst variable responses are seen in tumour necrosis factor alpha ( TNF alpha ) , IL 1 , IL 4 , IL 6 , and interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study we determined gene expression and secretion patterns for monocyte chemoattractant protein 1 ( MCP 1 / CCL2 ) and T cell specific CXCR 3 agonistic chemokines ( Mig / CXCL9 , IP 10 / CXCL10 , and 1 TAC / CXCL11 ) in TNF alpha , IFN gamma , and IL 1beta stimulated human alveolar epithelial cells type 2 ( AEC 2 ) . ^^^ IFN gamma , alone or in combination with IL 1beta and TNF alpha resulted in an increase in CXCL 10 , CXCL 11 , and CXCL 9 mRNA expression and generation of CXCL 10 protein by AEC 2 or A 549 cells . ^^^ CXCL 10 gene expression and secretion were induced in dose dependent manner after cytokine stimulation of AEC 2 with an order of potency IFN gamma > > IL 1beta > or = TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Glomerular T cell recruitment , CD40+ glomerular cells , and renal IFN gamma and TNF expression were similar in both chimeras and WT mice but significantly reduced in GM CSF / mice , indicating that either leukocyte or renal sources of GM CSF are sufficient to drive these aspects of the inflammatory response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cathepsin B death pathway contributes to TNF plus IFN gamma mediated human endothelial injury . ^^^ We report that TNF in the presence of IFN gamma activates Cat B as well as a caspase death pathway in both HUVEC and human dermal microvascular endothelial cells , but only activates caspase mediated death in HeLa cells and human embryonic kidney ( HEK ) 293 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The IL 1ra mediated suppression of clinical disease was associated with suppressed serum IFN gamma , TNF alpha , IL 1beta , IL 2 , IL 6 , C 3 , and anti AChR IgG 1 without influencing total serum IgG . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This effect is mediated by nitric oxide ( NO ) produced by the neuronal isoform of nitric oxide synthase ( NOS 1 ) , and is independent of the pro inflammatory cytokines interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of TGF beta , TNF alpha , IFN gamma , and CD 4 and CD 8 in the anastomosis was investigated . ^^^ This was paralleled by decreased expression of TGF beta ( stimulates healing ) and increased expression of IFN gamma and TNF alpha ( both inhibit healing ) ( p < . 05 ) . ^^^ No effect was seen on the expression of TGF beta , TNF alpha , IFN gamma , and CD 4 and CD 8 in colonic anastomoses . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
At various times after gel application , cell differentials and levels of inflammatory cytokines ( IL 1beta , IL 4 , IL 6 , IL 8 , IL 10 , IL 18 , IFN gamma , and TNF alpha ) in cervicovaginal lavage ( CVL ) fluid were monitored by flow cytometry and ELISA , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Plasma levels of the proinflammatory cytokines ( IL 1beta , IL 6 , TNF alpha , and IFN gamma ) , anti inflammatory cytokines ( IL 4 , IL 10 , and IL 1 receptor antagonist ) , and corticosterone were unaffected during the three reproductive stages after LPS challenge . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Multiple members of the TNF superfamily contribute to IFN gamma mediated inhibition of erythropoiesis . ^^^ We found that treatment of human erythroid precursor cells with IFN gamma up regulates the expression of multiple members of the TNF family , including TRAIL and the recently characterized protein TWEAK . ^^^ Because other members of the TNF family such as TRAIL and CD 95 ligand ( CD95L ) are known to interfere with erythroblast growth and differentiation , we investigated the role of different TNF / TNFR family proteins as potential effectors of IFN gamma in the immature hemopoietic compartment . ^^^ These results establish a new role for TWEAK and its receptor in normal and IFN gamma mediated regulation of hematopoiesis and show that the effects of IFN gamma on immature erythroid cells depend on multiple interactions between TNF family members and their receptors . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Liver injury induced by alpha GalCer injection into C57BL / 6 mice was accompanied by intrahepatic caspase 3 activity but appeared independent thereof . alpha GalCer injection also induces pronounced cytokine responses , including TNF alpha , IFN gamma , IL 2 , IL 4 , and IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our in vitro experiments elucidated that HGF constitutively produced CXCL 12 , and the levels were enhanced by stimulation with tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , transforming growth factor beta ( TGF beta ) , regulated upon activation normal T cell expressed and secreted ( RANTES ) and macrophage inflammatory protein 3 ( alpha ) ( MIP 3 ( alpha ) ) . ^^^ Flow cytometry analysis clarified that CXCR 4 was highly expressed on HGF , and CXCR 4 expression was abrogated by TNF alpha , IFN gamma and P . gingivalis LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interestingly , secretion of IFN gamma and TNF alpha were predominant in MDR patients who presented with bilateral pulmonary lesions and lung cavitation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RNase protection assay ( RPA ) was used to determine mRNA of woodchuck specific genes ( TNF alpha , IFN gamma , IL 15 , CD 3 , CD 4 , CD 8 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A direct correlation was observed between a higher survival rate and increased IFN gamma and TNF alpha production ( P < 0 . 05 ) in E . faecalis , B . vulgatus , and Peptostreptococcus associated mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
HcpA stimulated the release of high concentrations of the proinflammatory and Th 1 promoting cytokines interleukin 6 ( IL 6 ) and IFN gamma , in addition to significant levels of IL 12 , tumor necrosis factor alpha , and IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
On the other hand , systemic type 1 responses ( including gamma interferon [ IFN gamma ] , tumor necrosis factor alpha , and optimal cytotoxic T cell responses ) are likely to be critical for protection against the disseminated intracellular replication that occurs after mucosal invasion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Recombinant Ags 85A , 85B , and 85C induced significant lymphocyte proliferation as well as the production of gamma interferon ( IFN gamma ) , interleukin 2 ( IL 2 ) , IL 12 , and tumor necrosis factor alpha ( TNF alpha ) , but not IL 4 , from low and medium shedders . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effect of alanine , the iNOS ( inducible nitric oxide synthase ) inhibitor NMA ( N ( G ) methyl L arginine acetate ) or the iNOS and NADPH oxidase inhibitor DPI ( diphenylene iodonium ) on apoptosis induced by a pro inflammatory cytokine mix [ IL 1beta ( interleukin 1beta ) , TNF alpha ( tumour necrosis factor alpha ) and IFN gamma ( interferon gamma ) ] was additionally assessed by flow cytometry . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Dydrogesterone significantly inhibited the production of the Th 1 cytokines IFN gamma ( P= 0 . 0001 ) and TNF alpha ( P= 0 . 005 ) and induced an increase in the levels of the Th 2 cytokines IL 4 ( P= 0 . 03 ) and IL 6 ( P= 0 . 017 ) resulting in a substantial shift in the ratio of Th1 / Th2 cytokines . ^^^ CONCLUSION : Dydrogesterone inhibits the production of the Th 1 cytokines IFN gamma and TNF alpha from lymphocytes and up regulates the production of the Th 2 cytokines IL 4 and IL 6 , inducing a Th 1 to Th 2 cytokine shift . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the later phase of CNS infection and in a post drug treatment model , moderate to severe neuropathology was associated with high levels of IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CD56+ cells positively sorted by Microbeads demonstrated a significantly higher production of TNF alpha and IFN gamma after IL 2 stimulation than Dynabeads sorted cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both differentiated and dedifferentiated type 2 cells secreted MIP 2 , MCP 1 , and CINC 2 in response to a cytokine mixture of IL 1beta , TNF alpha , and IFN gamma or to IL 1beta alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Induction of thymocyte apoptosis by systemic administration of concanavalin A in mice : role of TNF alpha , IFN gamma and glucocorticoids . ^^^ Neutralization of the cytokines TNF alpha or IFN gamma , which protects mice from Con A induced hepatitis , prevents thymic atrophy as well as alterations in CD4+ and CD8+ cell numbers and apoptosis rates . ^^^ However , neither TNF alpha nor IFN gamma are detectable in thymocyte lysates after Con A injection , whereas both cytokines are present in liver , spleen and serum . ^^^ Con A induced thymic atrophy is accompanied by down regulation of Bcl 2 expression in the thymus , which is prevented by neutralization of TNF alpha or IFN gamma . ^^^ These data demonstrate that the thymus is a critical target organ of Con A induced inflammation ; the effects of Con A on the thymus are mediated by extrathymic production of TNF alpha and IFN gamma , but not by glucocorticoids . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression and activation of NOS 2 in normal adult human cerebral cortical astrocytes treated with three proinflammatory cytokines , IL 1beta , TNF alpha , and IFN gamma , are driven by two separable mechanisms . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The inflammation was confined to the colon , associated with increased cancer in situ , destruction of crypt architecture , and increased production of IL 1beta , TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Kinetics of phytohemaglutinin induced IFN gamma and TNF alpha expression in peripheral blood mononuclear cells from patients with chronic hepatitis B after liver transplantation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The level of IFN gamma , IL 4 and TNF alpha released by spleen lymphocytes stimulated with PPD was detected with ELISA . ^^^ Vaccination with pcD85B induced high level of IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Prolactin regulation of the expression of TNF alpha , IFN gamma and IL 10 by splenocytes in murine multiple low dose streptozotocin diabetes . ^^^ Spleen cell preparations depleted in erythrocytes and macrophages were stained for cytoplasmic TNF alpha , IFN gamma and IL 10 and analyzed with flow cytometry . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The present study was performed to analyze the responses of microglia to different forms of Abeta , with regard to release of the proinflammatory cytokines interleukin 1alpha ( IL 1alpha ) , IL 1beta , tumor necrosis factor alpha ( TNF alpha ) , IL 6 , and interferon gamma ( IFN gamma ) , as well as the IL 1 receptor antagonist ( IL 1ra ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this case , cells expressing TNF alpha alone as well as cells coexpressing TNF alpha and IFN gamma were found at significantly higher frequencies . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Colitis was evaluated by macroscopic and microscopic score ; the expression of NF kappaB P 65 was examined by immunohistchemical ( IHC ) ; the expression of TNF alpha mRNA was assayed by hybridization in situ ( ISH ) ; the cytokine TNF alpha , IL 4 , IFN gamma were estimated by enzyme linked immunoadsordent assay ( ELISA ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These events lead to the down modulation of c Jun , c Fos , Egr 1 , and NF kappaB transcription factors and thereby inhibit production of cytokines , e . g . , IL 2 , IL 4 , IFN gamma , and TNF alpha , upon TCR stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , NKG2D triggers the production of TNF alpha but not of IFN gamma , as well as the release of cytolytic granules by Vgamma 9 Vdelta2 T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Severity of spondylitis in F 2 mice positively correlated with serum levels of amyloid A , IL 6 , and Pg specific Abs , and showed negative correlation with Ag induced T cell proliferation , IFN gamma , IL 4 , and TNF alpha production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This was accompanied by reduced plasma IFN gamma and TNF levels during the initial stage of infection , followed by moderately lower VSG specific IgG2a Ab titers during the chronic stages of infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Blood from individuals heterozygous for the TLR 2 polymorphism Arg753Gln was tested for cytokine release upon stimulation with Borrelia lysate , and induction of TNF alpha and IFN gamma was significantly lower as compared with individuals not exhibiting this variation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma and TNF alpha responses to inactivated DEN Ags were detected in up to 0 . 54 and 1 . 17 % of total circulating CD4+ T cells , respectively . ^^^ The ratio of TNF alpha to IFN gamma producing CD4+ T cells was higher after stimulation with Ags from heterologous DEN serotypes . ^^^ IFN gamma and TNF alpha responses to individual HLA class 2 restricted peptide epitopes were detected in up to 0 . 05 and 0 . 27 % of CD4+ T cells , respectively . ^^^ TNF alpha to IFN gamma positive CD4+ T cell ratios varied between peptides , but the ratio of the sum of responses was highest against heterologous serotypes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha , IL 1beta IFN gamma , and IL 8 in the supernatant of Ana 1 were detected using radioimmunoassay ( RIA ) or enzyme linked immunosorbent assay ( ELISA ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Endotoxin levels were not correlated with allergen or mitogen induced IFN gamma , TNF alpha , or IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Finally , larger lesions were also correlated with a higher frequency of SLA specific inflammatory cytokine ( IFN gamma or TNF alpha ) producing lymphocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The CD 8 DCs secrete a large amount of IFN gamma , IL 12 and B lymphocyte stimulator / B cell activation factor of the tumor necrosis factor family in response to LPS and CpG stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PURPOSE : Previously we have reported that RT 4 , a well differentiated human bladder cancer line , increases the expression of macrophage derived chemokine ( MDC ) and interferon ( IFN ) gamma inducible protein 10 ( IP 10 ) in response to IFN gamma and tumor necrosis factor ( TNF ) alpha . ^^^ In this study we examined the signal mechanisms for inducting these 2 chemokines in RT 4 cells by lipopolysaccharide ( LPS ) , IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Despite the significant impact of LPS composition on the adhesion and phagocytosis of N . meningitidis no differences were found in terms of cytokine levels secreted by DC for IL 1 beta , IL 6 , IL 8 , TNF alpha , IFN gamma and GM CSF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Concentrations of IL 2 , IFN gamma , TNF alpha and IL 6 in mitogen stimulated PBMC supernatants of patients and controls were assessed by ELISA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We demonstrate BCL 2 related protein A 1 ( BCL2A1 ) mRNA expression in mesenchymal stromal cells ( MSCs ) that was up regulated by the inflammatory cytokines tumor necrosis factor alpha ( TNF alpha ) and / or interferon gamma ( IFN gamma ) . ^^^ Analysis of cytotoxicity and IFN gamma production illustrated that ACC 2 specific T cells did not recognize untreated MSCs or IFN gamma treated MSCs but showed specific recognition and killing of MSCs treated with TNF alpha plus IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
By IFN gamma and TNFalpha stimulus , these subunits are replaced by their counterparts LMP 2 , MECL 1 and LMP 7 , defined inducible subunits , thus originating the immunoproteasome , and expression of the proteasome activator PA 28 is enhanced . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The mRNA expression of 4 1BB , B7 . 1 , IFN gamma and TNF alpha of lymphocytes was up regulated and that of OX 40 and IL 10 was down regulated after pPD 1A administration . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Further , a strong time dependent secretion of cytokines IL 2 , IFN gamma and TNF alpha released from the SEB stimulated PBMC was shown . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Concomitant expression of interleukin 8 ( IL 8 ) , gamma interferon ( IFN gamma ) , IL 4 , transforming growth factor beta , and tumor necrosis factor alpha was seen in 8 / 10 H . pylori infected cases and in 5 / 10 noninfected cases ; all H . pylori infected subjects showed staining for at least two of the cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have reported recently that sodium butyrate suppressed IFN gamma , but not the LPS mediated induction of nitric oxide and TNF alpha in microglia via the specific inhibition of NF kappaB . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we determined the production of various neurotrophins [ brain derived neurotrophic factor ( BDNF ) , nerve growth factor ( NGF ) , glial cell line derived neurotrophic factor ( GDNF ) , neurotrophin 3 ( NT 3 ) and neurotrophin 4 ( NT 4 ) ] and some pro inflammatory cytokines [ tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) ] by unstimulated peripheral blood mononuclear cells ( PBMC ) in 21 relapsing remitting MS patients during different phases of disease ( stable , relapse and post relapse ) . ^^^ During acute phase of disease , we detected a considerable increase of BDNF , TNF alpha and IFN gamma production , while significantly higher levels of GDNF , NGF , NT 3 and NT 4 were found in post relapse phase . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Duodenal biopsy samples from 31 children with delayed CMA , 14 with celiac disease ( CD ) , and 14 healthy controls were studied for the spontaneous release of IFN gamma , TNF alpha , IL 2 , IL 4 , IL 5 , and IL 10 , measured by cytometric bead array , and of TGF beta and IL 6 measured by ELISA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) , interleukin 10 , and interferon gamma ( IFN gamma ) production by lipopolysaccharide stimulated peripheral blood mononuclear cells ( PBMCs ) as well as the expression of Toll like receptor 4 ( TLR 4 ) , TLR 2 , human leukocyte antigen D related ( HLA DR ) , and CD 16 on monocytes in 16 men and 9 women on the day before surgery were compared with measurements on postoperative day ( POD ) 1 . ^^^ Furthermore , TNF alpha production of PBMCs on POD 1 was significantly increased , and both IFN gamma production and HLA DR expression were significantly decreased compared with that observed before surgery in men , but no corresponding significant changes were observed in women . ^^^ Excessive TNF alpha and suppressive IFN gamma production of PBMCs , as well as a decrease in HLA DR expression on monocytes , occurred more often in men than in women after surgery , suggesting that these factors all contribute to an increased susceptibility of men to develop systemic inflammatory response syndrome or postoperative infectious complications . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The neutralization of either TGF beta or CTLA 4 resulted in similar increases in IFN gamma and TNF alpha serum concentrations in LPS challenged mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
One week after infection , serum interferon gamma ( IFN gamma ) and interleukin ( IL ) 10 levels in group BT were significantly lower , whereas serum IL 6 levels were significantly higher than in group T Serum TNF alpha levels in both group T and group BT were elevated at 1 wk after infection , although there was no significant difference between them . ^^^ Serum IFN gamma , IL 10 , and TNF alpha levels in group B were not elevated during the experimental term . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To summarize , in the chicken genome , we can identify the genes for 23 ILs , 8 type 1 interferons ( IFNs ) , IFN gamma , 1 colony stimulating factor ( GM CSF ) , 2 of the 3 known transforming growth factors ( TGFs ) , 24 chemokines ( 1 XCL , 14 CCL , 8 CXCL , and 1 CX3CL ) , and 10 tumor necrosis factor superfamily ( TNFSF ) members . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma activated macrophages on plastic continuously produced nitric oxide ( NO ) and TNF alpha during 72 hr culturing . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma prevents TNF alpha induced apoptosis in C2C12 myotubes through down regulation of TNF R 2 and increased NF kappaB activity . ^^^ Evidence has accumulated that the cytokine interferon gamma ( IFN gamma ) may act as a modulator of TNF alpha signalling . ^^^ Thus , the present study was designed to elucidate if TNF alpha can directly induce apoptosis in differentiated myotubes , to assess the potential anti apoptotic properties of IFN gamma and to get insight into the signalling pathways implicated in the modulatory effects of IFN gamma . ^^^ Together , these results demonstrate that TNF alpha directly induces apoptosis in differentiated myotubes and suggest that the cytokine IFN gamma , might represent a new immunoadjuvant therapeutic tool for managing cachexia . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increased TNF alpha ( P = . 025 ) and IFN gamma ( P = . 001 ) transcription also independently predicted for the eventual onset of extensive chronic GVHD . ^^^ Increased TNF alpha or IFN gamma transcription was not seen in either a syngeneic SCT recipient or 4 autologous SCT controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Heart tissue infiltrating cells from myocardium and valvular tissue produced TNF alpha , IFN gamma , IL 10 , and IL 4 , whereas few cells from valvular tissue produced IL 4 , showing that the lack of regulation in the valves could be responsible for the permanent and progressive valvular lesions . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
DCs were stimulated with the microbial agent Ribomunyl combined with IFN gamma and various inflammatory cytokine cocktails : TNF alpha / IL 1beta / IFN gamma and TNF alpha / PGE2 combined with monocyte conditioned medium ( MCM ) or IL 1beta / IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Quantitatively , BB gelatin stimulated adherent PEC of C3H / HeN mice to secrete interleukin ( IL ) 12 ( +p40 ) , TNF alpha , and IL 6 but not IL 1beta , IL 2 , IL 10 , and IFN gamma . ^^^ Qualitatively , BB gelatin induced secretion of KC , MIP 2 , MCP 1 , RANTES , and MIP 1a as well as IL 6 but not 6Ckine , CTACK , Eotaxin , G CSF , GM CSF , IL 2 , 3 , 4 , 5 , 9 , 10 , 12 , 13 , 17 , Leptin , IFN gamma , SCF , sTNFri , TARC , TNF alpha , TIMP 1 , Tpo , and VEGF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
At both 6 and 22 h after MCAO , activated spleen cells from stroke injured mice secreted significantly enhanced levels of TNF alpha , IFN gamma , IL 6 , MCP 1 , and IL 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mo DCs pulsed with rUreA activated allogenic CD56+ NK cells , as determined by TNF alpha and IFN gamma secretion , but not allogenic CD4+ / CD45RA+ nave T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have shown the increased response of neutrophils towards bacteria , the increased level of C 4 component of complement in blood , the rise of total antioxidant status of serum , and the predominance of Type 1 cytokine IFN gamma , TNF alpha and IL 2 production by peripheral blood mononuclear cells after shark liver oil intake . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both IFN gamma and TNF alpha positive T cells were visualized by flow cytometry . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the reproductive system , pathologies such as endometriosis , polycystic ovary syndrome , tubal obstruction , preeclampsia and recurrent abortions are related to the presence of inflammatory cytokines ( TNF alpha , IFN gamma , IL 1 ) and to high levels of free radicals that may damage biological molecules , such as lipids , proteins or DNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha , IFN gamma , IL 2 , IL 6 and IL 10 levels were detected by liquid chip . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The patients ' plasma inhibited the ability of IFN gamma to increase production of TNF alpha by both autologous and normal donor PBMCs , and recovery of exogenous IFN gamma from the patients ' plasma was greatly reduced . ^^^ The purified anti IFN gamma antibody was shown to be functional first in blocking the upregulation of TNF alpha production in response to endotoxin ; second in blocking induction of IFN gamma inducible genes ( according to results of high density cDNA microarrays ) ; and third in inhibiting upregulation of HLA class 2 expression on PBMCs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To elucidate the etiopathological role of IP 10 in type 2 DM , we measured the concentrations of IP 10 together with IFN gamma , TNF alpha , IL 18 , IL 6 and MCP 1 in plasma samples from 103 type 2 DM patients with various degrees of nephropathy . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Colonic tissue lesions of intestinal inflammatory disease patients seem to be related to the increased local production of pro inflammatory cytokines ( IL 1 , IL 6 , TNF alpha , and IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Additional neutralization of TNF in IFN gamma receptor deficient mice also did not attenuate CLP induced anemia . ^^^ Our results clearly demonstrate that neither IFN gamma alone nor in combination with TNF is a mediator of ACD in our model with transient anemia induced by protracted septic peritonitis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
They induce an IL 2 mediated primary T cell proliferation with a concomitant increase in IFN gamma production and decrease the density of TNF alpha induced cell surface adhesion molecules ICAM 1 , VCAM 1 , and E selectin on human umbilical vein endothelial cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this paper , the commitment of each leukocyte population of peripheral blood to the production of IFN gamma , TNF alpha , IL 12 , IL 4 , IL 5 and IL 10 was studied in patients with the CD form of Chagas disease . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although IFN gamma alone is sufficient for optimum expression of MHC , CD1d requires two signals , one provided by IFN gamma and a second mediated by microbial products or by the proinflammatory cytokine TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , the type 1 response ( IFN gamma , TNF alpha , IgG2a ) to TNP OVA induced by the diabetogen streptozotocin still developed in the absence of CD80 / CD86 costimulatory signaling . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , levels of leukotriene C ( 4 ) , PGE ( 2 ) , TNF alpha , IL 3 , IL 4 , IFN gamma , and IL 10 were comparable between the treated and untreated groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin 12p70 ( IL 12p70 ) , gamma interferon ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) production in the sera of mice fed these diets and challenged with Listeria monocytogenes were determined by enzyme linked immunosorbent assay . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Then the following experiments were done : ( 1 ) Survival rates of animals after every 6 hrs in the 72 hrs after modeling were recorded , serum and PLF levels of cytokines , including tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and interleukin 10 ( IL 10 ) , 6 hrs , 12 hrs , 24 hrs and 48 hrs after modeling were measured . ( 2 ) Model rats were treated with rIL 12 or SMI , the survival rate was recorded and serum levels of TNF alpha , INF gamma , and IL 10 before and after treatment were measured , and ( 3 ) amount of these cytokines produced by SM were determined 6 hrs , 12 hrs and 24 hrs after treatment . ^^^ RESULTS : Serum and PLF levels of IFN gamma , TNF alpha at all the time points in SP rats were significantly lower than those in MP rats while those of IL 10 6 hrs and 12 hrs after modeling were significantly higher in the former than that in the latter ( P < 0 . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Resting NK cells were induced to secrete tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) , and to kill target cells by engagement of specific , pair wise combinations of receptors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The specific production of IFN gamma and TNF alpha suggests that the NY ESO 1 specific CD4+ T cell clones belong to the Th 1 subtype . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When IFN alpha was combined with another antichlamydial cytokines , IFN gamma and tumor necrosis factor ( TNF ) alpha , the effect was synergistically enhanced . ^^^ Therefore , IFN alpha would act coordinately with other cytokines such as IFN gamma and TNF alpha , and play an important role in host defense against infection and in the establishment of persistent chlamydial infection of host , in which the organism remains viable , but in a culture negative state . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Their effects on macrophage functions were further assessed by expression analysis ( iNOS , IFN alpha , IFN gamma , TNF alpha , IL 1 , IL 10 , IL 12 , IL 18 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lymphocytes isolated from spleens or lung draining lymph nodes of treated animals were highly responsive to OVA and secreted TNF alpha , IFN gamma , and IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Intracellular production of cytokines by T cells was decreased only on postoperative day 1 ( 50 % for IL 2 , 29 % for IFN gamma and 22 % for TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Levels of TNF alpha and IFN gamma in culture supernatant of splenocytes were also determined . ^^^ Plasma levels of TNF alpha and IFN gamma were significantly reduced at 6 and 12 h after Con A injection , respectively , but the levels of IL 6 were increased at 6 h . ^^^ In vitro , beraprost also suppressed Con A induced TNF alpha production in splenocytes , while it stimulated IFN gamma production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vivo depletion of CD8+ T cells and NK cells decreased levels of IFN gamma , TNF alpha , and IL 12p40 in culture supernatants of LP cells in C57BL / 6 mice ( p < 0 . 01 ) . ^^^ In chronic colitis , weight loss and clinical scores were improved and levels of IFN gamma , TNF alpha , and IL 12p40 in culture supernatants of LP cells were also reduced in IL 15 KO mice ( p < 0 . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma sensitizes MIN6N8 insulinoma cells to TNF alpha induced apoptosis by inhibiting NF kappaB mediated XIAP upregulation . ^^^ Here , we found that : ( 1 ) XIAP level was inversely correlated with tumor necrosis factor ( TNF ) alpha induced apoptosis in MIN6N8 insulinoma cells ; ( 2 ) adenoviral XIAP overexpression abrogated the TNF alpha induced apoptosis through inhibition of caspase activity ; ( 3 ) downregulation of XIAP by antisense oligonucleotide or Smac peptide sensitized MIN6N8 cells to TNF alpha induced apoptosis ; ( 4 ) XIAP expression was induced by TNF alpha through a nuclear factor kappaB ( NF kappaB ) dependent pathway , and interferon ( IFN ) gamma prevented such an induction in a manner independent of NF kappaB , which presents a potential mechanism underlying cytotoxic IFN gamma / TNF alpha synergism . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cell function was measured by detection of IFN gamma , TNF alpha and IL 10 production . ^^^ RNA and dAMP cocultured with virus antigen significantly increased peripheral blood mononuclear cell secretion of IFN gamma , IL 10 and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vivo and in vitro , splenocytes of DBA / 2 mice produced various cytokines , such as IL 6 , TNF alpha , and IFN gamma in response to CAWS . ^^^ In contrast , anti GM CSF significantly reduced the production of TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : In 25 tumor / mucosa pairs , IL 1alpha , IL 2 , IL 4 , IL 5 , IL 15 , TNF alpha and IFN gamma were measured by real time PCR . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These mice also showed significant increases in serum TNF alpha and IFN gamma and decrease in serum IL 5 . ^^^ CONCLUSIONS : The results indicate that CpG ODNs aggravate Con A induced hepatitis by stimulating the production of T helper 1 ( Th 1 ) cytokines , TNF alpha and IFN gamma , suggesting that bacterial DNA that contains unmethylated CpG motifs may contribute to the exacerbation of immune mediated liver injury . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pheochromocytoma ( PC 12 ) cells differentiated with nerve growth factor ( NGF ) were co cultured with primary astrocytes and exposed to Mn and tumor necrosis factor alpha ( TNF alpha ) plus interferon gamma ( IFN gamma ) . ^^^ Pharmacologic inhibition of NOS 2 with ( + / ) 2 amino 5 , 6 dihydro 6 methyl 4H 1 , 3 thiazine ( AMT ) significantly reduced neuronal apoptosis , and the addition of low concentrations of the NO donor , S nitroso N acetylpenicillamine ( SNAP ) , to neurons cultured without astrocytes was sufficient to recover the apoptotic phenotype following exposure to Mn and TNF alpha / IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Isolated working hearts of iNOS ( / ) and wildtype mice were perfused for 120 min in the presence or absence of a mixture of pro inflammatory cytokines ( IL 1beta , TNF alpha , and IFN gamma ) . iNOS mRNA was detected only in cytokine treated wildtype hearts . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A large amount of cytokines such as perforin , nitric oxide , IFN gamma , IL 2 , TNF alpha were secreted in tumor tissue treated with tumor reactive TGF beta insensitive CD8+ T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The levels of interleukin ( IL ) 2 , interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , IL 6 , or IL 10 in the supernatants of T cell cultures were measured by cytokine enzyme linked immunosorbent assay ( ELISA ) kits . ^^^ However , THL could also significantly decrease the TT stimulated IL 2 production , the GtfD stimulated IL 2 , TNF alpha , IL 6 and IL 10 production , and the S . mutans stimulated IFN gamma , TNF alpha , and IL 10 production by T cells isolated from RAU patients . ^^^ Because RAU is probably a Thl mediated disease with elevated levels of IL 2 , IFN gamma , TNF alpha and IL 6 in either the patient ' s sera or oral lesions and these increased levels of cytokines can be reduced by THL , we suggest that THL may be a potential immunoceutical agent for treatment of RAU . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , an immunofluorescence analysis using anti porcine mAbs specific for IL 1beta , IL 2 , IL 4 , IL 6 , IL 12 , TNF alpha , and IFN gamma was performed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The anti IFN gamma IgG had in vitro biological activity on the IFN gamma dependent phosphorylation of STAT 1 as well as on the IFN gamma dependent up regulation of TNF alpha and IL 12 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the placenta , the Th 1 cytokines tumor necrosis factor alpha ( TNF alpha ) and gamma interferon ( IFN gamma ) are also synthesized in response to this pathogen , without fetal loss . ^^^ Here we show , by using mice homozygous for null mutations in either the cytokine or cytokine receptor genes , a requirement for both TNF alpha and IFN gamma signaling for an effective placental immune response to L . monocytogenes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Independent of IL 2 concentrations , large subject dependent differences in the mobilization of CD107a and expression of IFN gamma and TNF alpha by both NP and M 1 specific T cells were observed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When cocultured in vitro with SFMC , CD4+CD25+ T cells purified from either PB or SF were found to exert a considerable suppressive effect on the production of cytokines including TNF alpha , IFN gamma and interleukin 10 ( IL 10 ) . ^^^ The percentages of inhibition of each cytokines ranged from 41 . 8 to 98 . 4 % ( mean , 80 . 0 % ) for TNF alpha , 42 . 8 to 98 . 9 % ( mean , 83 . 2 % ) for IFN gamma and 59 . 3 to 96 . 6 % ( mean , 80 . 0 % ) for IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Previous studies from this laboratory reported that EGCG has marked suppressive activity on murine macrophages infected with the intracellular bacterium Legionella pneumophila ( Lp ) , an effect mediated by enhanced production of both tumor necrosis factor alpha ( TNF alpha ) and gamma interferon ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have recently shown that T cells and eosinophils cooperate in inducing bronchial EC apoptosis in asthma through secretion of IFN gamma and TNF alpha . ^^^ METHODS : Bronchial EC , cultured and exposed to IFN gamma and TNF alpha , were studied for the expression of adhesion molecules and apoptosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effect of IFN gamma and TNF alpha on the NADPH oxidase system of human colostrum macrophages , blood monocytes , and THP 1 cells . ^^^ The aim of this work was to analyze the effect of Interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) on NADPH oxidase activity and gp 91 phox gene expression in human colostrum macrophages ( CM ) , peripheral blood monocytes ( PBM ) , and myelomonocytic THP 1 cells . ^^^ We also investigated the effect of IFN gamma on the release of TNF alpha by these cells . ^^^ The addition of IFN gamma , alone or in combination with TNF alpha , increased spontaneous superoxide release by PBM and THP 1 cells ( p < 0 . 05 ) and increased phorbol myristate acetate ( PMA ) stimulated superoxide release by CM , PBM , and THP 1 cells ( p < 0 . 05 ) . ^^^ The NADPH oxidase activity of THP 1 cells consistently remained lower than that of CM or PBM , despite a dramatic response to IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The aim of this study was to investigate the possible influence of TNF alpha ( 308 ) , TGF beta ( codons 10 and 25 ) , IL 10 ( 1082 , 819 , 592 ) , IL 6 ( 174 ) and IFN gamma ( +874 ) polymorphisms on susceptibility to multiple sclerosis ( MS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pro inflammatory [ tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , IL 1 beta , IL 2 , IL 6 , IL 8 , IL 12 ] [ corrected ] and modulatory ( IL 4 , IL 10 ) cytokines were analyzed from cord blood , and at 6 , 24 [ corrected ] and 72 h postnatal age . ^^^ Premature rupture of membranes ( PROM ) was associated with higher levels of IL 2 at birth and at 6 h , of IFN gamma at 6 and 24 h postnatal age and of TNF alpha at 6 and 24 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 12 , TNF alpha , IFN gamma and IL 1beta expressions were significantly decreased , but IL 6 , IFN beta and IL 10 expressions were not affected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : The serum levels of IL 2 , IL 10 , TNF alpha , and IFN gamma in 2 hand transplant recipients were measured by enzyme linked immunosorbent assays ( ELISA ) . ^^^ RESULTS : The serum levels , IL 2 , TNF alpha , and IFN gamma in the 2 patients decreased significantly during the first posttransplant week , then increased gradually to the pretransplant levels , and finally decreased and maintained the low level . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vivo priming of wild type mice with P . acnes , but not with the selective TLR 2 ligands peptidoglycan and lipotheicoic acid , resulted in hepatocyte necrosis , hyperelevated serum levels of tumor necrosis factor alpha ( TNF alpha ) , interleukin ( IL ) 6 , interferon gamma ( IFN gamma ) , and IL 12 ( p40 / p70 ) , and increased RNA expression of proinflammatory cytokines ( IL 12p40 , IL 1alpha , IL 6 , IL 1beta , IL 18 , IFN gamma ) in the liver after a LPS challenge . ^^^ Furthermore , P . acnes priming sensitized TLR 2 deficient ( TLR 2 / ) but not MyD 88 / mice to LPS induced injury , evidenced by hepatocyte necrosis , increased levels of serum TNF alpha , IFN gamma , IL 6 , and liver proinflammatory cytokine mRNA expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Their influence on the mitotic division of the cells and on selected immunological parameters , e . g . , T and B lymphocyte proliferation and synthesis of the cytokines : interleukin 2 ( IL 2 ) , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) was assessed in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Participation of pro inflammatory and pro fibrogenic cytokines , such as tumor necrosis factor alpha ( TNF alpha ) , gamma interferon ( IFN gamma ) , and tumor growth factor beta ( TGF beta ) , in the pathogenesis of the lesions is herein studied . ^^^ Search for the in situ presence of TNF alpha and IFN gamma , was performed using rat IgG anti mouse antibodies against these cytokines . ^^^ The cytokines TNF alpha , IFN gamma , and TGF beta were detected in the cytoplasm of macrophages and in the necrotic material from 15 to 45 days post infection , decreasing their intensity until complete disappearance by the 65th day , which correlated with subsiding histopathological lesions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using intracellular cytokine staining we show herein that T cells will respond to short term ( 6 h ) activation with phorbol ester plus ionomycin by production of tumor necrosis factor ( TNF ) , IFN gamma or both . ^^^ Here CD 4 T cells preferentially produce TNF and CD 8 cells IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The bronchoalveolar lavage fluid concentrations of tumor necrosis factor alpha , gamma interferon ( IFN gamma ) , interleukin 6 ( IL 6 ) , IL 12 , KC ( functional IL 8 ) , monocyte chemotactic protein 1 , macrophage inflammatory protein 1alpha , monokine induced by IFN gamma , and IFN inducible protein 10 were significantly reduced in LBM 415 treated mice compared with the levels in placebo treated mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Some defects in the function of PKCtheta ( / ) T cells ( poor survival and reduced Bcl 2 and Bcl 10 ( L ) expression , CTL activity , and IFN gamma expression ) were partially or fully restored by coculture with wild type T cells or by addition of exogenous IL 2 , whereas others ( increased Bim ( EL ) expression and TNF alpha production ) were not . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In comparison with HNT TCR ( HNT ) transgenic mice , up regulation of IL 4 and IL 10 and down regulation of IFN gamma , IL 12 , and TNF alpha were observed in the influenza hemagglutinin ( 126 138 ) peptide stimulated splenocytes of HNT DcR 3 double transgenic mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found a substantial genetic contribution to the production of Th 1 cytokines such as IFN gamma and TNF alpha with heritabilities of 0 . 85 ( 95 % confidence intervals , 0 . 74 0 . 95 ) and 0 . 72 ( 0 . 50 0 . 93 ) , respectively , whereas no genetic influence on production of the Th 2 signature cytokine IL 4 was observed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor cell recognition was TCR and NKG2D dependent and induced TNF alpha and IFN gamma secretion by the clone ; accordingly , tumor targets expressed several NKG2D ligands , such as MHC class 1 chain related gene A and UL 16 binding protein molecules . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cigarette smoke extracts inhibit IL 2 , IFN gamma , and TNF alpha production in stimulated lymphocytes obtained from peripheral blood , even when the extracts were diluted 100 fold to 1000 fold . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha treated murine macrophages produced less IL 12p70 and IL 23 , after stimulation with IFN gamma and LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma , nitric oxide , and tumor necrosis factor were determined as potential mediators of Mtb growth control in AMs and AM / CD8 and AM / CD4 cocultures . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
But adding three of the proinflammatory cytokines commonly produced in the Alzheimer brain IFN gamma , IL 1beta , and TNF alpha along with Abeta ( 1 40 ) more than trebled NOS 2 expression and doubled NO production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IEL derived interferon gamma ( IFN gamma ) could partially account for this alteration , as a neutralizing antibody ( Ab ) against IFN gamma inhibited the upregulation of these molecules , except for CD 44 . ( 2 ) A marked fall in transepithelial electrical resistance was observed 4 h after IEL homing started , and Ab against IFN gamma slightly inhibited this fall in resistance . ( 3 ) The production of interleukin ( IL ) 8 and IFN gamma inducible protein 10 ( IP 10 ) , but not transforming growth factor ( TGF ) beta 1 or tumor necrosis factor ( TNF ) alpha , in the epithelial monolayer was markedly induced after IEL homing in a basolaterally polarized fashion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : It was found that GGA significantly reduced the clinical severity of colitis and suppressed the levels of MPO activity , TNF alpha and IFN gamma induced by DSS in the colon . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Confirmative to previous reports , conversion of precursor preadipocytes into mature adipocytes was completely inhibited upon exposure to TNF alpha , IL 1beta , IFN gamma , or TGF beta 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of MBCECs with LPS , induced E and P selectin expression , whereas TNF alpha or IFN gamma treatments did not . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The proinflammatory cytokines interleukin 1beta ( IL 1beta ) , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) are toxic to pancreatic beta cells and are implicated in the pathogenesis of type 1 diabetes . ^^^ Here we report that human ( h ) GH can prevent the apoptotic effects of IL 1beta , IFN gamma and TNF alpha in INS 1 and INS 1E cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In those animals treated with IL 10 aerosol , BALF levels of proinflammatory cytokines were reduced significantly compared with animals without IL 10 therapy ( TNF alpha , 87 % ; IL 1beta , 73 % ; IL 6 , 44 % ; IFN gamma , 39 % ; RANTES , 84 % ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Thus costimulation of MPhi with IFN gamma resulted in an increase of both NO synthesis and TNF expression by cells stimulated simultaneously by TLR 2 or TLR 4 agonists . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Renal immune cell populations [ total lymphocytes , macrophages , T , B , and natural killer ( NK ) cells ] and the expression kinetics of various related cytokine [ transforming growth factor beta ( TGF beta ) , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , interleukin ( IL ) 1 , IL 2 , IL 4 , IL 6 , IL 10 , IL 12 , and IL 13 ] , chemokine [ regulated upon activation , normal T cell expressed and secreted ( RANTES ) and monocyte chemoattractant protein l ( MCP 1 ) ] and T cell receptor beta ( TCR beta ) chain transcripts were studied serially during the course of the disease . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation with interferon gamma ( IFN gamma ) resulted in increased expression of B 7 H1 , whereas ICOS L expression was marginally increased upon stimulation with CD40L , with no effect of interleukin ( IL 1 ) , IL 17 , or tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Purified Lewis aortic endothelial cell cultures were pre treated with biochaninA or ethinylestradiol and stimulated with TNF alpha or IFN gamma for quantification of ICAM 1 / VCAM 1 and MHC 2 expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In an effort to better understand the chemical features that might explain the anti inflammatory properties of EP , we screened 15 commercially available compounds for cytoprotective or anti inflammatory effects using two in vitro assay systems : TNF and NO * production by lipopolysaccharide ( LPS ) stimulated RAW 264 . 7 murine macrophage like cells and changes in the permeability of Caco 2 human enterocyte like monolayers stimulated with a cocktail of pro inflammatory cytokines called cytomix ( 1000U / ml IFN gamma plus 10ng / ml TNF alpha plus 1ng / ml IL 1beta ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Hz and sHz significantly increased LPS and IFN gamma induced TNF alpha protein and transcripts in PBMC from animals with late stage SIV infection ( i . e . , AIDS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
From these results , Y 40138 may be expected as a new class of therapeutic drug for treatment of TNF alpha , IFN gamma and / or chemokine related liver diseases such as alcoholic liver disease . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MIF / mice displayed markedly elevated corticosterone levels and significant decreases in the inflammatory cytokines TNF alpha , IFN gamma , IL 2 , and IL 6 before , during , and after EAE onset . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma is considered an essential stimulus that allows macrophages to acquire activity against intracellular pathogens in response to a second signal such as TNF alpha . ^^^ However , protection against important pathogens can take place in the absence of IFN gamma through mechanisms that are still dependent on TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha but not IL 1 , IL 6 , IFN beta , and IFN gamma can mimic the effects of LPS although other factors apparently also contribute . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Children with AD had significantly lower spontaneous ( unstimulated ) production of regulatory cytokine IL 10 ( P < 0 . 001 ) , as well as IFN gamma ( P < 0 . 001 ) and TNF alpha ( P < 0 . 001 ) compared with the unaffected children . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Either IL 4 or IL 13 , but not IFN gamma or TNF , induced production of SCCA in keratinocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) and gamma interferon ( IFN gamma ) increased CR 3 surface expression , but only TNF alpha increased the ability of neutrophils to phagocytose B . pertussis , suggesting that elevated CR 3 expression alone is not sufficient to promote phagocytosis . ^^^ Tumor necrosis factor alpha ( TNF alpha ) and gamma interferon ( IFN gamma ) increased CR 3 surface expression , but only TNF alpha increased the ability of neutrophils to phagocytose B . pertussis , suggesting that elevated CR 3 expression alone is not sufficient to promote phagocytosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Strikingly higher interleukin ( IL ) 1alpha , IL 1beta and soluble interleukin 1 receptor antagonist ( sIL 1RA ) but similar IL 2 , sIL 2R , IL 3 , IL 4 , IL 6 , sIL 6R , IL 10 , tumour necrosis factor ( TNF ) alpha , transforming growth factor ( TGF ) beta and interferon IFN gamma urine levels in healthy females compared to healthy males : protection against urinary tract injury . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Four gene SNP sites of TNF alpha 238 , IFN gamma +874 , IL 4 590 and IL 10 1082 were determined by real time quantitative fluorescent polymerase chain reaction ( PCR ) . ^^^ No evident differences of TNF alpha 238 A were found between group 2 and control group ( chi ( 2 ) = 0 . 047 , P > 0 . 05 ) ; the significant differences of IFN gamma +874 A allele frequency were found between group 1 and group 2 ( chi ( 2 ) = 7 . 238 , P < 0 . 05 ) , and between group 1 and the control group ( chi ( 2 ) = 5 . 199 , P < 0 . 05 ) . ^^^ CONCLUSIONS : This study suggested the possibility that the TNF alpha 238 A allele and IFN gamma +874 A allele were associated with HBV intrauterine infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Ex vivo production of TNF alpha , IL 4 , IL 6 , IL 10 , and IFN gamma in whole blood cultures , and NK cell activity and peripheral blood NK cell , monocytes , T cell , and B cell percentages were measured in 42 outpatients with OCD who participated in a 12 week , double blind SRI trial . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , cross linking of IRp 60 on the eosinophils in the presence of IL 5 / GM CSF inhibited the antiapoptotic effect of these cytokines and blocked the release of TNF alpha , IL 1beta , IFN gamma , IL 4 , and 3T3 fibroblast proliferation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The MAPK activators IL 1beta and TNFalpha both induced hCG secretion from JAR cells , but not the Stat 3 activators IFN gamma and IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
On the other hand , pro inflammatory factors such as interferon gamma ( IFN gamma ) , endotoxin , tumour necrosis factor alpha ( TNF alpha ) and interleukin 1 beta ( IL 1beta ) , can be atherogenic by impairing RCT and cholesterol efflux , according to in vitro studies . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It has been reported that IFN gamma , TNF alpha , and IL 12 stimulate , and that IL 10 , TGF beta , and IL 4 suppress the protective immune response against tuberculosis . ^^^ Serum IL 12p40 , IL 4 , IL 10 , TNF alpha , IFN gamma , and TGF beta 1 levels were determined in 20 APTB cases ( group 1 ) before and 2 , 4 , and 6 months after therapy . ^^^ Before treatment , the mean serum IFN gamma , TNF alpha , and IL 10 levels in group 1 were statistically higher than those in group 2 ( P= . 001 , P= . 024 , P= . 016 , resp ) or group 3 ( P= . 003 , P= . 002 , P= . 011 , resp ) . ^^^ The levels in group 1 decreased significantly after treatment ( P= . 001 for IFN gamma , P= . 004 for TNF alpha , P= . 000 for IL 10 ) . ^^^ Because the serum IL 12p40 , IL 10 , TNF alpha , and IFN gamma levels were high in APTB , we believe that these cytokines have important roles in the immune response to Mycobacterium tuberculosis ( M tuberculosis ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum levels of TNF alpha , IFN gamma , IL 6 , IL 8 , IL 12 , IL 17 , and IL 18 in patients with active psoriasis and correlation with disease severity . ^^^ In this study , serum TNF alpha , IFN gamma , IL 6 , IL 8 , IL 12 , and IL 18 levels were significantly higher in active psoriatic patients than in controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunomodulatory drugs ( IMiDs ) are orally available small molecules that potently inhibit tumor necrosis factor alpha ( TNF alpha ) production by lipopolysaccharide ( LPS ) stimulated human peripheral blood mononuclear cells ( HuPBMCs ) but enhance secretion of such cytokines as interleukin 2 ( IL 2 ) and interferon gamma ( IFN gamma ) by stimulated T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interestingly , these data correlated with transient increases in TNF alpha , IFN gamma , IL 5 , and IL 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Irrespective of the presence or absence of asphyxia , sera concentrations of IL 2 , IL 4 , IFN gamma , and TNF alpha were higher in the neonates than those in the adults . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Hemodynamics , temperature and the course of plasma concentrations of TNF alpha , IL 1beta , IL 12 , IL 18 and IFN gamma were determined . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 6 , TNF alpha , and IFN gamma did not differ significantly between groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A selective role for the TNF p 55 receptor in autocrine signaling following IFN gamma stimulation in experimental autoimmune uveoretinitis . ^^^ We show that although T cell priming is relatively unaffected , macrophages lacking the TNF p 55 receptor fail to produce NO following IFN gamma stimulation because of a requirement for autocrine TNF alpha signaling through the TNF p 55 receptor . ^^^ Together , these results show that selected aspects of IFN gamma activation are controlled by autocrine secretion of TNF alpha , but that this control is lost in the presence of signals generated by pathogen associated molecular patterns recognizing receptors . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
T cells from myelin oligodendrocyte glycoprotein ( MOG ) 35 55 peptide primed Mac 1 deficient mice displayed an altered cytokine phenotype with elevated levels of TGF beta and IL 10 , but reduced levels of IL 2 , IFN gamma , TNF alpha , IL 12 , and IL 4 compared with control mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Ex vivo isolated NK cells neoexpressed the activation Ag CD 69 and released IFN gamma and TNF alpha upon binding with BMSC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This inhibition was relieved by neutralization of IFN gamma or of IFN gamma , TNF , and IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , inhibition of p 38 activity did not affect expression of the Th 1 cytokines IFN gamma and TNF induced by TCR stimulation , but decreased IL 12 mediated IFN gamma expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We used an enzyme linked immunosorbent assay to determine the concentrations of four proinflammatory cytokines [ tumor necrosis factor alpha ( TNF alpha ) , gamma interferon ( IFN gamma ) , interleukin 1 ( IL 1 ) and IL 6 ] in the same plasma samples . ^^^ The plasma concentrations of TNF alpha , IFN gamma , IL 1 and IL 6 were higher in microfilaremic women . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The high mortality of sepsis is in part mediated by bacterial endotoxin , which stimulates macrophages / monocytes to sequentially release early ( e . g . , TNF , IL 1 , and IFN gamma ) and late ( e . g . , HMGB 1 ) pro inflammatory cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CD4+ Th 1 cells produce IFN gamma , TNF alpha , and IL 2 . ^^^ When SPHK 1 expression was knocked down by SPHK 1 short interfering RNA , the production of IL 2 , TNF alpha , and IFN gamma by Th 1 cells in response to TCR stimulation was enhanced . ^^^ Consistently , overexpression of dominant negative SPHK 1 increased the production of IL 2 , TNF alpha , and IFN gamma in Th 1 cells . ^^^ Furthermore , overexpression of SPHK 1 in Th 1 and Th 0 cells decreased the expression of IL 2 , TNF alpha , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , quantitation of the CD 8 T cell response against one of these epitopes ( residues 261 270 from the phosphoprotein ) by MHC class 1 pentamer staining and by in vitro stimulation followed by intracellular IFN gamma and TNF alpha staining indicated that the majority of pulmonary CD 8 specific for the P 261 epitope were deficient in cytokine production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Compared with a panel of cytokines ( IL 1alpha , 1beta , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 15 , 16 , 18 , IFN gamma , GM CSF , and TNF alpha ) , IL 17 was as potent as IL 1alpha , 1beta , and TNF alpha , with a time and dose dependent phenomenon in stimulating CCL 20 expression in both well differentiated primary human and mouse airway epithelial cell culture systems . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , treatment with anti CX3CL1 mAb down regulated the mRNA expression of TNF alpha , IFN gamma , and perforin in the muscles . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using normal and fibrotic human lung fibroblasts and the human lung fibroblast cell line , MRC 5 , we examined the regulation of Fas induced apoptosis by the proinflammatory cytokines TNF alpha and IFN gamma . ^^^ IFN gamma did not sensitize cells to Fas induced apoptosis , but exhibited synergistic activity with TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RT PCR analysis showed that CLA up regulated the expression of TNF alpha , IL 1beta and IFN gamma genes in WEHI 3B JCS cells , which had previously been shown to play an important role in triggering the differentiation of myeloid leukemia cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum was analysed for the presence of IL 10 , TNF alpha , IL 8 , IL 6 , sIL 2R , IFN gamma , IFN alpha , IL 12 , IL 4 , sTNFRI ( p 55 ) , sTNFRII ( p 75 ) , IL 1RA , G CSF , sE selectin , sVCAM 1 , sICAM 1 and neopterin before initiation of and at several time points during IFN treatment and compared to 21 healthy controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There was a decrease in IFN gamma , IL 5 and TNF alpha levels comparing day 0 with day 28 in both groups , but no difference between the two therapy groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Production of IL 4 , IL 6 , TNF alpha , and IFN gamma in cord blood and in peripheral blood at 3 months was measured after 8 hour and 24 hour stimulation with phorbol ester plus concanavalin A . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
LLDT 8 ( 25 , 50 , 100 nM ) dose dependently reduced the production of Th 1 type cytokines ( IFN gamma , IL 2 ) and inflammatory cytokines ( TNF alpha , IL 6 ) in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RT PCR analysis showed that LPS activated IFN gamma expression gradually declined by CHL treatment in a dose dependent manner while mRNA production of TNF alpha , IL 2 , and FasL was not changed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Primary islets are not susceptible to cell death induced by TNF alpha alone ; however , they are killed by TNF alpha and IFN gamma in a nitric oxide dependent manner . ^^^ These islets were as susceptible as wild type islets to TNF alpha and IFN gamma induced cell death . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Some cells expressed TNF alpha and IFN gamma , but a much more intense proportion of TGF beta expressing cells were found , suggesting both a Th 1 and Th 3 patterns of immune response in yellow fever . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inflammatory cytokines including IL 1beta , TNF alpha and IFN gamma are implicated in the pancreatic islet beta cell death and functional loss during autoimmune diabetes and also seem to be involved in early loss of islet mass in islet transplantation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In culture medium with ripe DC the levels of such cytokines as IL 1b , IL 6 , IL 12 , IFN gamma , TNF alpha significantly increased and the production of IL 4 decreased . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Accordingly , Caco 2 cells or primary mouse intestinal epithelial cells ( IECs ) were incubated with `` cytomix ' ' ( a mixture of TNF , IL 1beta , and IFN gamma ) for various periods . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lung cytokine and chemokine production by BALB / c mice infected with human metapneumovirus ( hMPV ) was compared to respiratory syncytial virus ( RSV ) infected mice . hMPV infection induced lower levels of the inflammatory cytokines interleukin 1 ( IL 1 ) , IL 6 and tumor necrosis factor alpha but was a more potent inducer of granulocyte macrophage colony stimulating factor and triggered a more sustained production of the CXC chemokine KC compared to RSV . hMPV was a stronger inducer of both alpha interferon ( IFN alpha ) and IFN gamma responses than RSV . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro , the expression of B 7 H1 on TECs was increased after TECs were stimulated with IL 1alpha , LPS , TNF alpha , IFN gamma or anti CD 40 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine analysis of PSA peptide stimulated T cells per bead array assay exhibited specific IFN gamma and TNF alpha response in six of seven patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The percentage of CD4+T cells ( mean + / SD ) producing IFN gamma , IL 2 and TNF alpha were statistically significantly reduced in patients ( 19 . 1 + / 4 . 94 , 52 . 3 + / 20 . 86 and 12 . 8 + / 4 . 49 ) as compared to healthy controls ( 23 . 3 + / 3 . 67 , 67 . 5 + / 12 . 0 and 17 . 6 + / 5 . 96 respectively ) , ( p < 0 . 01 , 0 . 018 , 0 . 001 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neither the transcript nor RANTES itself was detected without prior stimulation of cells by tumor necrosis factor alpha ( TNF alpha ) and / or interferon gamma ( IFN gamma ) and production of RANTES was not induced by UVB ( 100 J / m2 ) irradiation alone . ^^^ Cells were irradiated with UVB just before addition of TNF alpha and IFN gamma to the medium and then cells and culture supernatants were harvested 12 , 24 , and 36 h later . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Administration of anti GITR mAb in a murine model of arthritis significantly exacerbated the severity and onset of joint inflammation with elevated production of TNF alpha , IFN gamma , IL 5 , and collagen specific IgG 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The immune state of the model was assessed by the lymphocyte infiltrative response and levels of TNF alpha , IFN gamma , IL 10 . ^^^ The levels of TNF alpha and IFN gamma were altered with the severity of EAE . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We also evaluated the effect of TNF alpha , IL 1 beta , interferon gamma ( IFN gamma ) , lipopolysaccharide ( LPS ) and conditioned media ( obtained from monocytes stimulated with LPS ) on the cytotoxicity of pre formed arylhydroxylamine metabolites in NHEK . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interestingly , MSP induced increase in IL 1 , TNF alpha and IFN gamma mRNA expression was evident in females , but not in males . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It was determined that in ICOS / MRL Faslpr as compared with the WT strain , ( 1 ) there is a significant reduction in circulating IgG and double stranded DNA autoantibody isotype titers , and ( 2 ) there is an amplification of the frequency of intrarenal T cells generating IFN gamma and TNF alpha in ICOS / versus WT mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The coexpression of CKRs ( CCR 2 , CCR 3 , CCR 5 , CXCR 3 , and CXCR 4 ) and intracellular cytokines ( interleukin 10 [ IL 10 ] , IL 4 , tumor necrosis factor alpha [ TNF alpha ] , and gamma interferon [ IFN gamma ] ) on T CD4+ and CD8+ peripheral cells from individuals with indeterminate ( IND ) or cardiac ( CARD ) clinical forms of Chagas ' disease after in vitro stimulation with Trypanosoma cruzi antigens , were evaluated in this study . ^^^ The percentage of T CD4+ and CD8+ cells coexpressing CCR 5 and IFN gamma , CXCR 3 and IFN gamma , and CXCR 3 and TNF alpha were higher in CARD than in IND individuals ; on the other hand , the percentage of T CD4+ or CD8+ cells coexpressing CCR 3 and IL 10 or coexpressing CCR 3 and IL 4 were lower in CARD individuals than in IND individuals . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Splenocytes from coinfected C57BL / 6 mice produced higher levels of gamma interferon ( IFN gamma ) and tumor necrosis factor alpha than splenocytes from mice infected with either pathogen alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CD 4 T cells became primed and accumulated in secondary lymphoid organs in the absence of PKCtheta , but had severely diminished IFN gamma , TNF , and IL 17 production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Five weeks after booster , immune lymphocytes were still producing large amounts of cytokines including IFN gamma , IL 2 , IL 4 , IL 10 and TNF alpha and the antibody titres were still similar to those titres recorded 1 week post booster . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Macrophage invasion into endoneurium was investigated and Schwann cell proliferation was followed as well as the expression of cytokines IL 1beta , IL 10 , IFN gamma and TNF alpha mRNA . ^^^ At this time point marked expression of TNF alpha and IFN gamma mRNA was observed in the endo and epi / perineurium of the proximal stump . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Apigenin induces apoptosis in Hep G 2 cells : possible role of TNF alpha and IFN gamma . ^^^ Apigenin induced programme cell death in terms of TNF alpha , IFN gamma release and induction of caspases activity . ^^^ TNF alpha and IFN gamma levels in apigenin pretreated groups were significantly and dose dependently elevated as compared to the control values ( 28 39 % and 66 85 % ) , ( 208 336 % and 579 1088 % ) , respectively . ^^^ The effects on caspases , TNF alpha , and IFN gamma processes mediate the plausible mechanism of apoptosis induction of apigenin . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RAG 1 / mice exhibited significantly ( p < 0 . 05 ) lower levels of plasma TNF , IFN gamma , and IL 12 during infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Ex vivo intracellular cytokine staining demonstrated that two important inflammatory cytokines , IFN gamma and TNF alpha , were being made by these T cells recovered from the pancreas 6 days following adoptive transfer . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , although MoDCs matured with TNF and PGE 2 selectively produced the inhibitory IL 12p40 subunit at steady state , they were able to produce the bioactive IL 12p70 heterodimer after stimulation with CD 40 ligand and / or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A ten fold lower dose ( 0 . 5 microg instead of 5 microg ) of CpG ODN in nanoparticles was adequate to obtain levels of IFN gamma , TNF alpha , and IL 2 comparable to those observed following immunisations in saline . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1beta and / or TNF alpha plus IFN gamma induce beta cell apoptosis via the activation of beta cell gene networks under the control of the transcription factors NF kappaB and STAT 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , sophoridine significantly enhanced mRNA expression of IL 10 and IFN gamma , but decreased TNF alpha mRNA expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Skin derived T cell clones from pustular skin reactions were positive for CCR 6 but negative for CCR 8 and secreted high amounts of CXCL 8 and GM CSF , often together with IFN gamma and TNF alpha after in vitro stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To investigate whether these oscillations are under a genuine circadian control , we assessed the daily expression of clock genes ( Per 1 , Per 2 , Clock , and Bmal 1 ) , a clock controlled gene ( Dbp ) , cytolytic factors ( granzyme B and perforin ) , and cytokines ( IFN gamma and TNF alpha ) in NK cells enriched from rats maintained in constant darkness ( DD ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We also studied the regulation of factor H synthesis in keratinocytes by several cytokines , namely IL 1alpha , IL 2 , IL 6 , TGF beta 1 , TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In order to investigate the molecular mechanisms participated in the process of marrow failure , we created an in vitro model of hematopoietic cell suppression , by continuous addition of TNF alpha and IFN gamma in an vitro long term bone marrow culture system . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of inducible nitric oxide synthase ( iNOS ) and generation of nitric oxide in response to IFN gamma and TNF alpha is important in control of infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These cells were CD 45 RA ( ) , CD 69 ( ) , CD 45 RO ( + ) , CD 95 ( + ) , and intercellular CTLA 4 ( + ) , and secreted low levels of TNF alpha and IL 10 , but no IL 2 , IL 4 , IL 5 , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , CD62L expression did not correlate with differences in the abilities of CTLs to proliferate or produce interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) in vitro in response to Env peptide stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation of peripheral blood mononuclear cells ( PBMCs ) with OK 432 ( 1 micro / ml ) for 24 h induced Th 1 type cytokines ( IFN gamma , TNF alpha , IL 12 , IL 18 ) as well as IL 10 and TGF beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study , we investigated whether various cytokines ( interleukin 1 [ IL 1 ] , IL 6 , IL 10 , tumor necrosis factor alpha [ TNF alpha ] , interferon alpha [ IFN alpha ] , and IFN gamma ) are able to influence PCV 2 infection in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Differential effects of TNF alpha and IFN gamma on gene transcription mediated by NF kappaB Stat 1 interactions . ^^^ Regulation of gene transcription by the cytokines tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) involves complex interactions between NF kappaB and Stat families of transcription factors . ^^^ Further , NF kappaB and Stat 1 proteins directly regulate transcription by interacting cooperatively on NF kappaB SIE DNA binding in response to TNF alpha plus IFN gamma . ^^^ In contrast , IFN gamma inhibits TNF alpha induced transcription of an NF kappaB reporter gene in a Stat 1 dependent mechanism in 2fTGH fibroblasts . ^^^ IFN gamma and Stat 1 dependent inhibition of NF kappaB transcription occurs independent of TNF alpha induced NF kappaB DNA binding . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The production of IFN gamma ( interferon gamma ) , TNF alpha ( tumor necrosis factor alpha ) , IL ( interleukin ) 10 , IL 5 , IL 4 , and IL 2 by lymphocytes was estimated by using a CBA system . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha , IFN gamma , IL 1 , and IL 6 levels were higher in mice that had been inoculated with P . murina at all three time points . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The refractory shock was distributive with most cytokines ( IFN gamma , TNF alpha , IL 2 , IL 4 , IL 6 and IL 8 ) peaking 3 h after infusion and coinciding with the onset of the shock . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Evidence indicated that transforming growth factor beta and other important cytokines such as IL 1 , TNF and IFN gamma might be involved in evoking the antioxidant enzymes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cells were exposed to three doses of a cytokine mixture ( maximal concentration : IL 1beta 50 U / ml ; TNF alpha 1 , 000 U / ml ; IFN gamma 1 , 000 U / ml ) or vehicle and analysed for the total cell number ( 2 h intervals ) and timing of each cell death and division . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In microglia stimulated with LPS and IFN gamma , nicergoline suppressed the production of superoxide anions , interleukin ( IL ) 1beta , IL 6 , and tumor necrosis factor ( TNF ) alpha in a dose dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Chronic TNF alpha exposure led to suppression of subsequent TCR stimulation ( e . g . , TCR / CD28 induced proliferation , cytokine production ( IFN gamma , TNF alpha ) ) but left TCR independent restimulation unaffected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , the treatment of C 6 cells with ESW reverts the decrease of nNOS activity and NO production induced by a mixture of lipopolysaccharides ( LPS ) , interferon gamma ( IFN gamma ) plus tumour necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Production of chymase , tryptase , IL 4 , IL 5 , IL 13 , TNF alpha and IFN gamma mRNA was measured by real time RT PCR . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the course of our study , we examined the effects of triptolide on stimulated murine macrophages and demonstrated that triptolide inhibited superoxide anion production , NO production , and the expression of iNOS mRNA and the mRNA of some key inflammation related cytokines , namely TNF alpha , IL 1beta , IL 6 and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Anti IFN gamma and anti TNF alpha work in similar ways , but the latter brings serious complications in some patients . ^^^ Anti IFN gamma was in some ways superior to anti TNF alpha , which was ineffective in MS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
EPS 1 induced IL 12 , IFN gamma , IFN alpha , TNF alpha and IL 18 , but not IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Modulation of IFN gamma production by TNF alpha in macrophages from the tumor environment : significance as an angiogenic switch . ^^^ Upregulation of IFN gamma production by the addition of LPS or TNF alpha was observed in macrophages from the tumor bearing peritoneal cavity . ^^^ RT PCR analysis revealed external TNF alpha induced IFN gamma gene expression in macrophages from tumor environment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We therefore studied the effect of estradiol , medroxyprogesterone acetate ( MPA ) , RU 486 , gamma interferon ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , 12 O tetradecanoylphorbol 13 acetate ( TPA ) and calcium ionophore A 23187 on the expression in the non expressing endometrial tumor cell lines Hec1B and Skut1B , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Diabetes led to an increase in urinary excretion of monocyte chemoattractant protein 1 ( 705 % of control ) , TNF alpha ( 1 , 586 % of control ) , IFN gamma ( 298 % of control ) , kidney fibronectin mRNA ( 457 % of control ) , and glomerular infiltration of macrophages ( 764 % of control ) , effects significantly reduced by ATL146e treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Polaprezinc suppressed the increase of MPO activity and the production of TNF alpha and IFN gamma in the colon tissues of mice with DSS induced colitis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Astilbin in vivo remarkably induced IL 10 expression in lymph node cells at an earlier time and decreased TNF alpha and IFN gamma expression at a later time . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
No differences were detected between the patients and controls with regard to IFN gamma and TNF alpha secreted by their PMBC , nor were there differences in the serum levels of IgG anti myosin antibodies . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both EPO and CEPO equivalently decreased the EAE associated production of TNF alpha , IL 1beta and IL 1Ra in the spinal cord , and IFN gamma by peripheral lymphocytes , indicating that their action involves targeting neuroinflammation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have investigated intracellular production by T cells and plasma levels of TNF alpha , IL 2 and IFN gamma in 12 active and 10 inactive Takayasu ' s arteritis ( TA ) patients and 12 healthy controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , in vitro studies revealed that IFN gamma stimulated LRG 47 KO macrophages display defective intracellular killing of amastigotes despite normal expression of TNF and NO synthetase type 2 and that both NO synthetase type 2 and LRG 47 are required for optimum IFN gamma dependent restriction of parasite growth . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found that IL 18 induced the production of MCP 1 in macrophages , which was IL 12 independent and was not mediated by autocrine cytokines such as IFN gamma or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , the production of proinflammatory cytokines , such as TNF alpha , IL 1beta , IL 6 , IFN gamma , IL 12 , and IL 18 , was markedly down regulated in ginsan treated mice compared with those of control infected mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This effect was potentiated by IFN gamma , or TNF alpha , or IFN gamma plus IL 1beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Serum TNF alpha , IL 6 and IL 18 levels of patients with TA were significantly higher than those of controls ( P < 0 . 05 ) , but IFN gamma and IL 12 levels were not . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This type of response involves participation of alveolar macrophages and T CD4+ , CD8+ and T gammadelta lymphocytes , and production of cytokines such as IL 2 , IFN gamma , IL 12 , IL 18 and TNF alpha , as well as chemokines such as RANTES , MCP 1 , MIP 1alpha and IL 8 which play an important role in the migration of different cell subpopulations to the infection site for the formation of granulome . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum levels of IFN gamma , IL 6 , TNF alpha , IL 5 and ECP were increased significantly at HS but mildly at cefaclor induced eruptions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Ten daily injections of 400 ng IL 23 , starting on the first day of MLD STZ administration led to significant and sustained hyperglycemia along with weight loss compared with controls ( no IL 23 ) , and a significant increase in the number of infiltrating cells , a lower insulin content , enhanced apoptosis , expression of IFN gamma and IL 17 ( not seen in the controls ) and a significant increase in the expression of TNF alpha and IL 18 in the pancreatic islets . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mouse models implicate overproduction of TNF alpha by antigen specific CD 8 T lymphocytes in pathogenesis and strong type 1 CD 4 and CD 8 T lymphocyte responses , synergistic activities of IFN gamma and TNF alpha , and IgG2a antibodies in immunity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Unlike splenocytes from wild type mice , splenocytes from HDC / mice do not produce histamine in response to the myelin Ag , whereas production of IFN gamma , TNF , and leptin are increased in HDC / splenocytes in comparison to those from wild type mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Either DC became effectively dye coupled when activated with LPS plus IFN gamma or TNF alpha plus IFN gamma . ^^^ LPS plus IFN gamma induced dye coupling was mediated by DC derived TNF alpha . ^^^ In addition , CpG plus IFN gamma induced dye coupling in BMDCs , which was also mediated by DC derived TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found that CD4+CD161 and CD8+CD161 subsets contained predominantly naive T cells that secreted high levels of IL 2 after in vitro stimulation , and CD4+CD161int and CD8+CD161int subsets contained predominantly effector and central memory T cells that secreted high levels of IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The synergy between anti CD 40 and CpG was evidenced by increased production of IFN gamma , IL 12 , TNF alpha , and NO by Mphi , as well as by augmented apoptogenic effects of Mphi against tumor cells in vitro . ^^^ The activation of cytotoxic Mphi after anti CD 40 plus CpG treatment was dependent on IFN gamma but not TNF alpha or NO , and did not require T cells and NK cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Induction of iNOS was accomplished by treatment with cytomix ( TNF alpha , IL 1beta , and IFN gamma each at 5 ng / ml ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Rat pretreatment with anti PSGL 1 Ab prevented hepatic insult in a model of cold ischemia , followed by OLT , as assessed by 1 ) decreased hepatocellular damage ( serum glutamic oxaloacetic transaminase / glutamic pyruvic transaminase levels ) , and ameliorated histological features of ischemia / reperfusion injury , consistent with extended OLT survival ; 2 ) reduced intrahepatic leukocyte infiltration , as evidenced by decreased expression of P selectin , ED 1 , CD 3 , and OX 62 cells ; 3 ) inhibited expression of proinflammatory cytokine genes ( TNF alpha , IL 1beta , IL 6 , IFN gamma , and IL 2 ) ; and 4 ) prevented hepatic apoptosis accompanied by up regulation of antiapoptotic Bcl 2 / Bcl xL protective genes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The temporal response of microdialysis probes containing antibody immobilized microspheres against five different cytokines ( tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , interleukin 2 ( IL 2 ) , IL 4 , and IL 5 ) to concentration changes outside of the probe was also determined . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The production of interleukin 1 beta ( IL 1beta ) , IL 4 , IL 5 , IL 6 , IL 8 , IL 10 , IL 12p70 , IL 13 , tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , IFN gamma inducible protein 10 ( IP 10 ) , monocyte chemoattractant protein 1 ( MCP 1 ) , Regulated on Activation Normal T cell Expressed and Secreted ( RANTES ) and prostaglandin E 2 ( PGE 2 ) were quantified by enzyme linked immunosorbent assays . ^^^ P . gingivalis induced the secretion of the pro inflammatory cytokines IL 1beta , TNF alpha , IL 6 and IFN gamma , the chemokines IL 8 , RANTES and MCP 1 and the inflammatory mediator PGE 2 in an ex vivo human whole blood model . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 15 is known to up regulate the production of proinflammatory cytokines , IL 1beta , IL 8 , IL 12 , IL 17 , IFN gamma and TNF alpha and has an inhibitory effect on activation induced cell death . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In saline controls , IED feeding decreased IL 1beta , IL 5 , IL 6 , TNF alpha , and IFN gamma and increased IL 10 compared with CD fed animals . ^^^ In septic animals , IED feeding increased IL 5 and IL 6 , while decreasing IFN gamma and IL 10 in the distal third of the small intestine compared with CD fed septic rats , whereas IL 1beta and TNF alpha levels were unchanged . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MAIN OUTCOME MEASURES : Numbers of unstimulated and mitogen activated cytokine secreting cells ( IFN gamma , interleukin [ IL ] 2 , IL 4 , IL 6 , IL 10 , IL 12 , and TNF alpha ) were measured by ELISPOT assay at frequent intervals , and correlations were sought with CD4+ and CD8+ cell counts and viral loads . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The intracellular cytokines IL 2 , TNF alpha , and IFN gamma were measured in PMA ionomycin stimulated T cells ( CD4+ ) , while CD 54 , CD 95 , CD 86 , CD 25 , CD 69 , and CD 71 were measured in pokeweed mitogen stimulated B cells , by flow cytometry . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In confluent monolayers in 3 mum cell culture inserts , we assessed trans EC Evans Blue ( EB ) conjugated albumin leak under basal , unstimulated conditions and following stimulation with either lipopolysaccharide or a mixture of equal concentrations of TNF alpha , IL 1beta and IFN gamma ( cytomix ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The polymorphic frequencies of the T cell signaling genes CD 45 , CD40L and CTLA 4 , and the cytokine genes TNF alpha , IFN gamma , IL 10 and TGF beta 1 were studied . ^^^ RESULTS : Multivariate analysis showed no significant association between acute rejection and single nucleotide polymorphisms in CTLA 4 , TGF beta 1 , IL 10 or TNF alpha genes or dinucleotide repeat polymorphisms in IFN gamma and CD40L genes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Sera from TG nude mice with keratitis reacted with alpha internexin on Western blot analysis , and the T cells of these mice on stimulation with alpha internexin exhibited proliferation responses and produced IL 2 , IFN gamma , and TNF alpha , but not IL 4 or IL 5 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PURPOSE : The purpose of this study was to determine levels of IL 2 , 4 , 5 , 10 , TNF alpha , and IFN gamma in aqueous humor ( AH ) from patients with active panuveitis , anterior uveitis ( AU ) , and noninflammatory controls by using a flow cytometric mutiplex array ( CBA ) and to compare with results from ELISA . ^^^ Correlations between IFN gamma and IL 10 were found in all uveitis groups ( P < 0 . 01 ) but not in controls , whereas TNF alpha correlations with IL 4 / IFN gamma were obtained in controls but not in the uveitis groups ( P < 0 . 01 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Deltaacr infected mice also showed high serum levels of TNF alpha , IFN gamma and G CSF , suggesting that Acr may play a role in modulating the host response to infection . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , astrocytes were found to constitutively and predominantly transcribe , translate and shed TNFR 1 rather than TNFR 2 , but TNFR 2 expression was increased by adding TNF alpha , IL 1 , and IFN gamma , but not by adding LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Following cytokine ( IFN gamma or tumor necrosis factor alpha ) and / or LPS treatment , the majority of human cell lines induced IDO but failed to produce NO . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
By contrast , these cells positively regulated the secretion of IL 4 and IFN gamma production and negatively regulated the secretion of tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A lethal human septic shock model , mouse generalized Shwartzman reaction ( GSR ) , was elicited by two consecutive lippolysaccharide ( LPS ) injections ( 24 h apart ) in which interferon gamma ( IFN gamma ) induced by interleukin ( IL ) 12 played a critical role in the priming phase , and tumor necrosis factor ( TNF ) was an important effector molecule in the second phase . ^^^ Although the PBMC from children produced a substantial amount of IFN gamma after IL 12 priming , the GSR response , with augmented TNF production and an up regulated TLR 4 expression of macrophages , was not elicited by LPS stimulation . ^^^ CD56+natural killer cells , CD56+T cells , and CD57+T cells ( NK T cells ) , which age dependently increased in PBMC , produced much larger amounts of IFN gamma after IL 12 priming than that of conventional CD 56 CD57 T cells and also induced cocultured macrophages to produce TNF by subsequent LPS stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MATERIAL AND METHODS : IL 1beta , IL 2 , IL 4 , IL 5 , IL 6 , IL 8 , IL 10 , IL 12 p 40 , IL 13 , IL 15 , IL 17 , IFN alpha , IFN gamma , TNF alpha , GM CSF , MIP lalpha , MIP 1beta , MCP 1 , eotaxin and RANTES were measured in serum using multiplex bead arrays . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Intra tracheal infection of Balb / c mice with either mce mutant induced lower but progressive production of IFN gamma and TNF alpha , as well as larger delayed type hypersensitivity ( DTH ) reactions , than their parental H37Rv strain . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lipopolysaccharide ( LPS ) was used to stimulate monocyte derived pro inflammatory ( IL 1beta , TNF alpha , IL 12 ) and anti inflammatory ( IL 10 ) cytokines , whilst concanavalin A ( Con A ) was used to stimulate T cell ( Th ( 1 ) : IFN gamma and Th ( 2 / 3 ) : IL 10 ) cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , RANKL increased the expression of TNFalpha , MCP 1 , and IL 6 but not of IL 10 , IL 12 , IFN gamma , and iNOS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study attempts to correlate the frequency of infection by Candida with the following immunological factors presented in cancer patients undergoing chemotherapy : neutrophil number ; level of exogenous myeloperoxidase ( MPO ) ; cytokines IL 12 , IL 10 , TNF alpha and IFN gamma . ^^^ We observed that : 1 ) MPO deficiency seems to be an important risk factor for invasive candidiasis independently of the neutrophil count ; 2 ) the levels of cytokines in serum showed disturbances in cancer patient group ; 3 ) in vitro production of TNF alpha , IFN gamma and IL 10 by WBC is stimulated in cancer patients and to various degrees may be connected with previous Candida colonization or infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In coculture and artificial skin equivalent experiments , subsets of T regulatory cells neither induced keratinocyte death nor suppressed apoptosis induced by skin T cells , T ( H ) 1 cells , IFN gamma , or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Arthritogenic cells secreted IFN gamma and TNF alpha ( but not IL 4 ) when stimulated with Con A in vitro , and pretreatments of recipient rats with either anti IFN gamma or a recombinant TNF alpha receptor before transfer ameliorated arthritis development . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Long term incubation of epithelia with IFN gamma alone or in combination with TNF alpha increased tau to 0 . 49 and 0 . 59 min , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Further studies on inflammatory mediators such as IFN gamma , IL 12 , TNF alpha , IL 1beta and NO showed down regulation , whereas no inhibition was observed in the case of anti inflammatory cytokine IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Production of interferon gamma ( IFN gamma ) , interleukin 2 ( IL 2 ) , IL 4 , and tumor necrosis factor alpha ( TNF alpha ) was measured using multiparametric flow cytometry . ^^^ Cortisol alone and in combination with GH Ab decreased CD4+ and CD8+ cells producing IFN gamma , TNF alpha and IL 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : LAEC and SAEC were treated with IFN gamma , TNF alpha , IL 1beta , or their combination . ^^^ RESULTS : 1 ) ICAM 1 expression on both LAEC and SAEC was significantly increased by IFN gamma stimulation alone and synergistically enhanced by IFN gamma plus TNF alpha or IL 1beta stimulation , 2 ) intranuclear displacement of Stat 1 in SAEC by the stimulation with IFN gamma plus TNF alpha or IL 1beta was recognized earlier in comparison with that by IFN gamma stimulation alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine ( IL 5 , IL 6 , IL 10 , IL 13 , IFN gamma and TNF alpha ) responses to allergens ( egg ovalbumin ( OVA ) , beta lactoglobulin ( BLG ) , house dust mite ( HDM ) ) , vaccines ( tetanus toxoid ( TT ) ) , diphtheria toxoid ( DT ) ) , intestinal flora ( heat killed Lactobacillus ( HKLB ) ) , heat killed Staphylococcus aureus ( HKSA ) , Staphylococcus aureus enterotoxin B ( SEB ) and mitogen ( phytohaemaglutinin ( PHA ) ) were compared . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , levels of IL 2 , IL 3 , IL 7 , IL 12 ( p 40 and p 70 ) , 15 , TNF alpha , IFN gamma and GM CSF were not affected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Multiplex assays for cytokines ( IFN gamma , IL 2 , IL 4 , IL 5 , TNF alpha , and IL 13 ) in breast milk were negative . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When Th 1 effector CD 4 cells encounter tolerizing Ag in vivo , their capacity to express the effector cytokines IFN gamma and TNF alpha is lost more rapidly than noneffector functions such as IL 2 production and proliferation . ^^^ IFN gamma and TNF alpha expression were both partially rescued when TCR proximal signaling was bypassed , indicating that both TCR proximal and distal signaling defects impair the expression of these two effector cytokines . ^^^ Enforcing T bet expression during tolerization selectively rescued the ability to express IFN gamma , but not TNF alpha . ^^^ Conversely , expression of a dominant negative T bet in Th 1 effectors selectively impaired the ability to express IFN gamma , but not TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using direct sequencing and microsatellite genotyping , we examined 23 polymorphisms in 11 cytokine genes including the IL 1alpha , IL 1beta , IL 1 receptor antagonist , IL 4 , IL 6 , IL 8 , IL 10 , IL 13 , IL 4 receptor , TNF alpha and IFN gamma genes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A treatment of neonatal rat ventricular cardiomyocytes with interleukin 1 beta ( IL 1beta ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) induces apoptosis via an NO dependent pathway . ^^^ Treatment of myocytes with IL 1beta , TNF alpha , and IFN gamma induced a significant increase in the formation of peroxynitrite , and a pretreatment with catalase was found to quench the production of peroxynitrite . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To model this , we stimulated murine primary astrocytes with a complete inflammatory cytokine mix consisting of TNF alpha , IL 1beta and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The T helper ( Th 1 ) deviated cells produce detectable level of tumor necrosis factor ( TNF alpha ) , interleukin 2 ( IL 2 ) and interferon gamma ( IFN gamma ) , while the Th 2 deviated cells produce significant amount of interleukin 4 ( IL 4 ) and interleukin 5 ( IL 5 ) . ^^^ Oral administration of UA at doses of 10 , 20 , 40 , 80 and 160 mg kg ( 1 ) per oral dose inhibited the presence of IL 2 , IFN gamma and TNF alpha in the peripheral blood . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , serum levels of MIF in adult dengue patients with different disease severity and clinical outcome were determined and compared with the levels of other cytokines , tumor necrosis factor alpha ( TNF alpha ) , interleukin 6 ( IL 6 ) , IL 10 , and interferon gamma ( IFN gamma ) , in the same patients . ^^^ Serum levels of MIF , IL 6 , and IL 10 , but not IFN gamma or TNF alpha , were higher in all DHF patients who died than in DHF survivors and DF patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Whereas IFN gamma and IFN beta were strong inducers of IRF 1 mRNA ( > 10 fold ) in a time and dose dependent manner , TNF alpha and IL 1beta also induced IRF 1 mRNA to a lesser extent ( 2 to 3 fold ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study assessed the number of CD 4 T lymphocytes , the parasitemia and serum levels of interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 ( IL 1 ) , IL 4 and IL 10 of patients infected by human immunodeficiency virus ( HIV ) and human immunodeficiency virus / Chagas ' disease coinfection . ^^^ Serum levels of IFN gamma , IL 4 and TNF alpha were significantly higher in patients with HIV / Chagas ' disease . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of treatment were monitored by determining arthritis and histological scores and measuring cellular proliferation , production of cytokines ( IL 2 , IL 10 , IL 12 , IL 6 , IFN gamma , and TNF alpha ) and IgG , and the expression of mRNAs for inducible nitric oxide synthase ( iNOS ) , monocyte chemoattractant protein 1 ( MCP 1 ) , and CXCR 3 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : We measured the concentrations of interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 2 ( IL 2 ) , IL 4 , IL 6 , IL 10 , and soluble TNF receptor 1 ( sTNFR 1 ) in the serum and cerebrospinal fluid ( CSF ) of 23 patients with SSPE in Papua New Guinea ( PNG ) , a country with a high incidence of SSPE , and Japanese controls by cytometric bead array or ELISA . ^^^ There were no differences in serum IFN gamma , TNF alpha , IL 2 , IL 4 , and sTNFR 1 , or CSF IFN gamma , TNF alpha , IL 2 , IL 4 , IL 10 , and sTNFR 1 levels between the affected patients and controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of mRNA of 8 chemokines was examined using RT PCR , before and after TNF alpha , IFN gamma , and IL 1beta stimulation . ^^^ Stimulation with TNF alpha , IFN gamma , and IL 1beta upregulated MIP 3alpha mRNA expression and induced its protein secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Gene regulation was studied by treating endothelial cells with TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Intracellular expression of IL 2 , IL 4 , IL 5 , IFN gamma , and TNF alpha in peripheral CD4+T lymphocytes was determined using flow cytometry . ^^^ However , a significant difference in the proportion of CD4+T lymphocytes producing IL 2 ( 39 . 2 versus 21 . 8 % ) , IFN gamma ( 19 . 5 versus 7 . 8 % ) , and TNF alpha ( 35 . 8 versus 16 . 1 % ) between Groups 1 and 3 could be detected , whereas the distribution of IL 4 and IL 5 producing CD4+T lymphocytes was similar . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Indeed , tumour necrosis factor alpha ( TNF alpha ) was identified as a significant contributor to ISS mediated activation of NK cells and was observed to act in an additive fashion with IFN alpha in the induction of IFN gamma from NK cells and to up regulate CD 69 expression on NK cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MKP 1 / mice are hyperresponsive to low dose LPS induced toxicity and exhibit significantly increased serum TNF alpha , IL 6 , IL 12 , MCP 1 , IFN gamma , and IL 10 levels after systemic administration of LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We examined the levels of serum cytokines including T helper type 1 ( Th 1 ) associated cytokines [ IFN gamma , TNF alpha , and interleukin ( IL ) 2 ] , Th 2 associated cytokines ( IL 4 , 5 and 6 ) and regulatory T cell associated cytokines ( IL 10 and TGF beta 1 ) in 94 ATLL patients , 39 asymptomatic human T cell lymphotropic virus type 1 ( HTLV 1 ) carriers and 50 healthy adult volunteers , to clarify whether elevated levels of particular cytokines are associated with the prognosis of ATLL patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Patients with chronic hepatitis C genotype 3 were analyzed for single nucleotide polymorphisms of interleukin ( IL ) 10 , IL 1 beta , interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) and transforming growth factor beta ( TGF beta ) by polymerase chain reaction using sequence specific oligonucleotide primers . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine concentration in children with Fa and Hp infection is significantly different in IFN gamma , IL 2 , IL 8 , and TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To test the hypothesis that this mechanism of IDO regulation is active in epithelial cells , HeLa cells were treated with IFN gamma , TNF alpha , or IL 1beta to determine optimal cytokine concentrations and time for maximal cytokine receptor expression . ^^^ Flow cytometric analysis with antibodies to receptors for IFN gamma , TNF alpha , or IL 1beta indicated that each cytokine upregulated expression of the other cytokine receptors by 4 h , with maximal expression observed between 16 and 20 h after cytokine treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The results show that p 17 , while ineffective on resting monocytes , exerts an inflammatory action on IL 4 mediated inhibition of TNF alpha and IFN gamma production induced by IL 15 stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha and IFN gamma inversely modulate expression of the IL 17E receptor in airway smooth muscle cells . ^^^ The present study shows that airway smooth muscle cells express IL 17BR in vitro and that its expression is upregulated by TNF alpha and downregulated by IFN gamma . ^^^ In addition , both IFN gamma and dexamethasone are able to antagonize a TNF alpha induced IL 17BR increase in mRNA expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Rather , two type 1 cytokines exert dominant and counterregulatory roles : tumor necrosis factor alpha ( TNF alpha ) , acting via the type 1 TNF alpha receptor , promotes fibrin deposition , while gamma interferon ( IFN gamma ) , acting via STAT 1 and IFN gamma receptors expressed on radioresistant cells , suppresses fibrin deposition . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Glucosamine sulfate inhibits TNF alpha and IFN gamma induced production of ICAM 1 in human retinal pigment epithelial cells in vitro . ^^^ METHODS : ARPE 19 cells were used as a model to determine the effects of GS on the expression of the ICAM 1 gene upregulated by TNF alpha or IFN gamma , by Western blot analysis and semiquantitative reverse transcription polymerase chain reaction ( RT PCR ) . ^^^ RESULTS : Both TNF alpha and IFN gamma increased the expression of ICAM 1 at the mRNA and protein levels in a time and dose dependent manner in ARPE 19 cells . ^^^ GS effectively downregulated the TNF alpha or IFN gamma induced expression of ICAM 1 in the protein and mRNA level in a dose dependent manner . ^^^ GS further inhibited the nuclear translocation of p 65 proteins in TNF alpha and phosphorylated STAT 1 in IFN gamma stimulated ARPE 19 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
With zinc consumption , TNF alpha and IL 1beta expression was greater in activated monocytes and granulocytes , and IFN gamma mRNA levels were higher in activated T lymphocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha and IL 6 concentrations were not elevated , however , there was a tendency for an elevation of IFN gamma concentrations in the thalamus / basal ganglia . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this system , TNF alpha dependent control of growth of the avirulent strain H37Ra was independent of inducible nitric oxide synthase ( iNOS ) and interferon gamma ( IFN gamma ) , as shown by the infection of macrophages from selected gene disrupted mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Hepatic mRNA expression of TNF alpha , IFN gamma and intercellular adhesion molecule 1 ( ICAM 1 ) were determined by semiquantitative RT PCR . ^^^ Serum levels of TNF alpha and IFN gamma were measured by enzyme linked immunosorbent assays ( ELISA ) . ^^^ Administration of NF kappaB decoy ODNs significantly suppressed NF kappaB activation as well as mRNA expression of TNF alpha , IFN gamma and ICAM 1 in the liver graft . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Plasma interleukin ( IL ) 2 , IL 4 , tumor necrosis factor alpha ( TNF alpha ) and interferon ( IFN gamma ) were not detectable 3 d after CLP . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 10 , IL 12 , interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) were determined in serum and LILMCs by ELISA . ^^^ Stimulated IFN gamma and TNF alpha levels in LILMCs were increased in both groups . ^^^ Histological activity index ( HAI ) and stage had a negative correlation with TNF alpha and IFN gamma levels in group 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We analyzed cytokine gene expression and gene polymorphisms in infants with and without IUGR in Pakistan , where IUGR is very common . 45 IUGR and 55 control mother / infant pairs were studied . mRNA for IL 10 , IL 8 , TNF alpha , TGF beta , IL 6 , IL 4 , IL 1beta , IL 12 , IFN gamma and GAPDH was quantified with RT PCR from placenta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Various growth factors and cytokines including TGF beta , IFN gamma , or the activators of the death receptors , TNF alpha and FasL , also induce apoptosis in AECs . ^^^ Preincubation with IFN gamma or a zinc deficiency increases the percentage of apoptotic AECs in response to TNF alpha or FasL , suggesting that AECs have mechanisms to protect them from cell death . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effect of polyester vascular prostheses on synthesis TNF alpha , IFN gamma and NO by human peripheral blood leukocytes in vitro ] . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These skin infiltrating lymphocytes might trigger , by the local production of Th 1 cytokines such as TNF alpha and interferon gamma ( IFN gamma ) , the eruption of psoriatic plaques in patients with a genetic predisposition for psoriasis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , hearts transduced with RANTES 9 68 showed a significant ( P < 0 . 05 ) reduction in cell infiltration and intragraft expression of TNF alpha , IFN gamma , endogenous RANTES , and TGF beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The lethal shock was abolished by the administration of anti IFN gamma or TNF alpha antibody . ^^^ Taken together , alpha GalCer sensitization rendered mice very susceptible to LPS mediated lethal shock , and IFN gamma and TNF alpha were found to play a critical role in the preparation and execution of the systemic lethal shock , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Effect of liangxue huoxue xiaoyin tang on serum levels of TNF alpha , IFN gamma and IL 6 in psoriasis of blood heat type . ^^^ To explore the effect of Liangxue Huoxue Xiaoyin Tang ( LHXT Decoction of Removing Heat from the Blood and Promoting Blood Flow to Eliminate Psoriasis ) on serum levels of tumor necrosis factor alpha ( TNF alpha ) , interferon ( IFN gamma ) and interleukin 6 ( IL 6 ) in psoriasis of blood heat type . ^^^ Blood samples from both the treatment group ( N=33 ) and control group ( N=30 ) were taken before and after treatment , and the serum levels of TNF alpha , IFN gamma and IL 6 were determined by radio immunoassay and ELISA . ^^^ The remarkably high serum levels of TNF alpha , IFN gamma and IL 6 in patients before treatment ( P < 0 . 01 ) were obviously decreased after one course of treatment ( P < 0 . 05 ) and were close to those of healthy subjects after two course of treatment ( P > 0 . 05 ) . ^^^ The data demonstrate that LHXT has the actions of reducing serum levels of TNF alpha , IFN gamma and IL 6 in psoriasis of blood heat type , and may exert a pharmacological effect targeting at the cytokines . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found a significant diurnal variation for IFN gamma and IL 8 , and a tendency for TNF , all inversely correlated to the serum cortisol levels , but no evidence for such a rhythm for IL 1beta and IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Activated APCTCM induced secretion of almost equal amounts of IFN gamma , TNF alpha and IL 2 as DCims indicating induction of Th 1 differentiation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Abrogation of HuR function by use of inhibitory peptides , or marked reduction of HuR levels by RNA interference , prevents LFA 1 engagement mediated stabilization of T cell TNF alpha or IFN gamma transcripts , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We also found that NK cells were directly rapidly activated by the heat treated Ags from M . tuberculosis ( H37Ra ) in vitro ; in turn , the activated NK cells improved gammadelta T cell proliferation both by CD 54 mediated cell cell contact through the forming immune synapse and by soluble factors TNF alpha , GM CSF , and IL 12 , but not IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here , we assess the quality of the HIV specific CD 8 ( + ) T cell response by measuring 5 CD 8 ( + ) T cell functions ( degranulation , IFN gamma , MIP 1beta , TNF alpha , and IL 2 ) simultaneously in chronically HIV infected individuals and elite nonprogressors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression levels of IL 6 , IL 8 , IL 10 , TNF alpha , IFN gamma and IL 1beta were measured by quantitative real time RT PCR ( qRT PCR ) . ^^^ Univariate analysis of the 84 cases for development of the prediction model showed that IL 6 , IL 8 , TNF alpha and IL 1beta were risk factors for mortality and IL 10 and IFN gamma were protective factors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The supernatant from dendritic cells stimulated with H . pylori for 18 h contained twofold higher levels of IL 12p70 than IL 10 and induced the proliferation of syngeneic splenocytes and type 1 T helper cell cytokine release ( IFN gamma and TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increased frequencies of PPD stimulated CD3+ cells expressing IL 2 , IFN gamma , and TNF alpha in TB were seen when compared with frequencies of controls . ^^^ The presence of type 1 cytokine biased responses in TB patients was supported by a shift in the distribution pattern of cytokine expression from exclusively IL 2 or TNF alpha expression seen in controls towards an increased frequency of IFN gamma / IL 2 or IFN gamma / TNF alpha co expression in TB . ^^^ Non specific stimulation with PMA and ionomycin revealed increased frequencies of CD4+ cells expressing IFN gamma in controls , while expression of IL 2 , IL 4 , IL 10 , IL 13 , and TNF alpha was not different . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Defects in AM function may be related to diminished levels of IFN gamma and TNF alpha postinfection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Levels of intracellular MIP 1beta , RANTES , tumor necrosis factor alpha , and IFN gamma produced by NK CD 56 ( bright ) cells were significantly higher in HIV 2 than HIV 1 infected subjects with high CD 4 ( + ) T cell counts but fell to similar levels as CD 4 counts dropped . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To evaluate variables influencing in vitro immune response induction , pig monocyte derived DCs ( moDCs ) were treated with putative type 1 and type 2 antigens ( Ags , killed Mycobacterium tuberculosis ( Mtb ) and hen egg white lysozyme ( HEWL ) ) and recombinant porcine cytokines ( IL 6 , IL 10 , IL 12 , IFN gamma and TNF alpha ) . ^^^ Addition of TNF alpha , IFN gamma or IL 12 to HEWL treated moDCs increased IL 12p35 and reduced IL 13 mRNA ; suggesting a DC 1 phenotype . ^^^ This DC 1 bias was enhanced by TNF alpha , IFN gamma or IL 12 , which increased IL 12p35 and to a lesser extent IL 10 mRNA but reduced IL 13 mRNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of mRNAs for IFN gamma , IL 18 , and TNF alpha was reduced in the colon . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using a low dose infection C57BL / 6 mouse model , we have demonstrated that host defense requires immune mechanisms involving CD 4 T cell mediated , TNF alpha , IL 12 , and IFN gamma dependent , macrophage activation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Low levels of IDO , at both mRNA and protein levels , was detected in the normal cornea , up regulated by IFN gamma and TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here we show that IFN gamma and LPS synergistically induce the expression of proinflammatory factors , including interleukin 1 ( IL 1 ) , IL 6 , IL 12 , NO , and tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In basilar arteries of infected animals versus controls , mRNAs encoding for IL 6 , iNOS , IFN gamma , IL 2 , MCP 1 , IL 1beta , IL 10 , TNF alpha , CCR 1 , MMP 9 , TGF beta , as well as MMP 9 protein in CSF , were found to be significantly up regulated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
T cells stimulated in the absence of polarizing cytokines secreted modest amounts of IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation with variant peptides also altered the relative frequencies of the various subsets of cells that expressed IFN gamma , TNF alpha , MIP 1beta , and combinations of these cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Indeed , when we examined the cytokine profile of a cohort of filaria infected ( n = 10 ) and uninfected ( n = 10 ) individuals in response to live infective stage larvae or microfilariae of Brugia malayi , we found significant impairment of both Th 1 and Th 2 cytokines characterized by diminished production of IFN gamma , TNF alpha , IL 4 , IL 5 , and IL 10 in infected patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These results suggest that CD 40 CD154 interaction regulates IFN gamma independent mechanisms of host protection through induction of macrophage antimicrobial activity and modulation of TNF alpha signaling . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although IFN gamma production in the spleens of IOE infected TNFR I / II / and TNF alpha depleted mice was higher than that detected in wild type controls , these mice had higher bacterial burdens than infected controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
HRC 203 had greater anti viral activity on both isolated hepatocytes and macrophages , whereas both ribavirin and HRC 203 inhibited production of the pro inflammatory cytokines interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) by macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OBJECTIVES : To evaluate the presence , concentration , and short term variability of TH 1 and TH 2 mediated cytokines ( interferon gamma [ IFN gamma ] , tumor necrosis factor alpha [ TNF alpha ] , interleukin 10 [ IL 10 ] , IL 5 , IL 4 , and IL 2 ) in EBC of children with asthma or CF and in controls and to analyze the discriminating ability of inflammatory markers in EBC between children with asthma or CF and controls . ^^^ In controls , IFN gamma , TNF alpha , and IL 10 were detected in 9 % , 14 % , and 3 % , respectively ; IL 2 , IL 4 , and IL 5 were not detected in controls . ^^^ CONCLUSIONS : Cytokines such as IFN gamma , TNF alpha , IL 10 , IL 5 , IL 4 , and IL 2 can be detected in EBC of children with asthma or CF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Functionally , the SIV specific CD8+ T lymphocytes were cytotoxic ; secreted IFN gamma , tumor necrosis factor alpha , and macrophage inflammatory protein 1beta ; and had an activated effector phenotype . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Results showed that a significant enhancement of proliferative and cytotoxic activities accompanied with increased IFN gamma and TNF alpha production as well as decreased IL 4 production were obtained from the mice vaccinated with 4T1 ITAC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Reduced lipopolysaccharide triggered cytokine production ( of IL 12p40 , IFN gamma , IL 6 and TNF alpha ) by draining lymph node cells of mice sensitized and challenged with DPCP indicated that no adaptation is induced . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The levels of IFN gamma and TNF alpha secreting CD8+ T cells were higher in splenocytes from DNA LACK / MVA LACK than in DNA LACK / VV LACK immunized animals . ^^^ Moreover , protection against L . major was significantly higher in DNA LACK / MVA LACK than in DNA LACK / VV LACK immunized animals when boosted with the same virus dose , and correlated with high levels of IFN gamma and TNF alpha secreting CD8+ T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We used quantitative RT PCR , ELISA and immunocytochemistry in cultured SGECs from eight patients with pSS and eight controls on treatment with IL 10 , tumor necrosis factor alpha ( TNF alpha ) , IFN alpha and IFN gamma . ^^^ Treatment with IFN alpha , IFN gamma and TNF alpha + IFN gamma increased the level of BAFF mRNA in pSS patients ( the mean increases were 27 fold , 25 fold and 62 fold , respectively ) and in controls ( mean increases 19 . 1 fold , 26 . 7 fold and 17 . 7 fold , respectively ) , with no significant difference between patients and controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Nitrite detection , IFN gamma knockout mice , TNF alpha neutralization , ELISA , aminoguanidine ( AG ) treatment , MPO , and plate counts were done . ^^^ RESULTS : Evidence shows ( 1 ) without IFN gamma , nitrite levels are elevated , ( 2 ) neutralization of TNF alpha does not change nitrite levels , and ( 3 ) absence of IFN alpha and reduced NO synergistically increases disease progress and upregulates pro inflammatory cytokines , PMN number , and bacterial load . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There were significantly decreased IFN gamma and tumour necrosis factor ( TNF ) alpha mRNA levels , and a tendency toward higher levels of transforming growth factor beta 1 mRNA in HLA DRB1 * 0301 positive compared with HLA DRB1 * 0301 negative patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Under stimulation by bacterial antigens , release of IL 10 was highly suppressed compared to that of IFN gamma and TNF alpha ; IL 4 was undetectable . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Substantial differences between ATCC 27139 and ATCC 27139 J1R strains were observed in the capacity to induce innate cytokines such as TNF alpha , IL 12 , IL 18 , and IFN gamma , and pathogen associated molecular pattern receptors , TLR 2 and Nod 2 , by spleen cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Evaluation of cytokines ( MIG , IFN gamma , TNF alpha , IL 4 , IL 5 , and IL 10 ) during the different evolutive phases of chagasic esophagopathy . ^^^ The production of cytokines ( MIG , IFN gamma , TNF alpha , IL 4 , IL 5 , and IL 10 ) was studied in 39 individuals , including 28 with chagasic esophagopathy and 11 nonchagasic patients with gastroesophageal reflux disease . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1alpha , IL 1beta , IL 2 , IL 5 , IL 9 , IL 12 ( p 40 ) , IL 12 ( p 70 ) , IL 13 , IL 17 , GM CSF , IFN gamma , MCP 1 and TNF alpha levels were significantly higher in serum ; IL 4 , G CSF , eotaxin , KC and RANTES exhibited the opposite trend . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pre treatment of mice with lentinan resulted in increased concentrations of TNF alpha , IL 12 and IFN gamma and also an increased number of L . monocytogenes specific CD 8 T cells in the spleen . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The impact of age on intracellular Type 1 ( IFN gamma and TNF alpha ) and Type 2 ( IL 4 ) cytokines , after stimulation with PMA / ionomycin , was determined in three CD4+ T subsets , i . e . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NK cells were analyzed by flow cytometry for intracellular TNF alpha , interferon gamma ( IFN gamma ) , and IL 4 and IL 10 cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CD 45 ( RO / + ) cells produce more TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The capability of CD 4 ( + ) and CD 8 ( + ) T cells to produce IFN gamma , IL 2 and TNF alpha had reached normal ranges from 2 months post SCT onwards . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro production of TNF alpha by yeast stimulated splenocytes from mice intravenously infected with the low virulence C . albicans PCA 2 strain was not affected in TLR 4 defective mice , but the TNF alpha production in response to hyphae was higher in TLR 4 defective than in control animals ; the production of IFN gamma by these splenocytes was similar to controls , as well as the frequency of IFN gamma producing CD4+T lymphocytes , indicating that TLR 4 defective mice are capable of mounting a Th 1 adaptive immune response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 10 was positively correlated with IFN gamma , TNF and necrosis area in pleural sections . ^^^ IFN gamma and TNF levels were lower in regressive disease , as well as reduced necrosis area in pleural sections . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Cultured HASMCs were stimulated with IL 1beta , TNF alpha , and IFN gamma and treated with ( R ) enantiomers and ( S ) enantiomers of albuterol and formoterol , with and without propranolol and ICI 118 , 551 , and in combination with dexamethasone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The first pattern was seen in SCID mice and in mice with deleted alpha / beta T receptor , TNF R 1 , IL 12 , IFN gamma , or iNOS genes , and showed a massive INPG with a high granulomatous infiltration of the lung , a large and homogeneous eosinophilic necrosis full of acid fast bacilli , with marked karyorrhexis , coarse basophilic necrosis , and surrounded by patches delimited by partially conserved alveolar septum full of PMNs . ^^^ Local expression of IFN gamma , iNOS , TNF and RANTES showed no significant differences between these mouse strains generating a discrete INPG . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Herein , we have shown that compared to L . major , L . amazonensis induced higher parasite burden associated to similar IL 4 , IFN gamma , and TNF alpha mRNA expressions and IFN gamma and IL 10 levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Induction by TNF alpha or CD40L , but not with IL 4 , IFN gamma and TGF beta , resulted in increased number and complexity of both types of membrane projections . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Database analysis suggested that the following four pathways were involved in alteration of HSCs toward a quiescent phenotype in response to IFN gamma : 1 ) down regulation of the TGF beta and PDGF signaling pathways ; 2 ) reorganization of intermediate filaments ; 3 ) up regulation of fatty acid metabolism ; 4 ) decreased expression of TNF alpha converting enzyme ( TACE ) / a disintegrin and metalloproteinase 17 ( ADAM 17 ) , which is responsible for shedding of the proinflammatory cytokine TNF alpha . ^^^ TNF alpha mRNA / protein expression was not altered by IFN gamma treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Development of immunity in vaccinated mice was associated with the generation of leukemia specific cytotoxic T lymphocytes ( CTLs ) and secretion of cytokines TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Supernatant levels of interleukin 2 ( IL 2 ) , IL 4 , IL 5 , IL 6 , IL 10 , IL 12 , IL 13 , IL 17 , interferon gamma ( IFN gamma ) , granulocyte / macrophage colony stimulating factor , and tumor necrosis factor alpha ( TNF alpha ) , were determined by multiplex technique , and intracellular cytokine staining ( ICS ) was employed to detect IFN gamma , IL 2 , IL 4 and IL 13 in CD3+ cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , the effect of GH on interferon gamma ( IFN gamma ) and lipopolysaccharide ( LPS ) induced production of nitric oxide ( NO ) , interleukin ( IL ) 12p70 , IL 12p40 , tumor necrosis factor alpha ( TNF alpha ) , and IL 6 were examined using mouse peritoneal macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Probiotics and commensals reverse TNF alpha and IFN gamma induced dysfunction in human intestinal epithelial cells . ^^^ Both TNF alpha and IFN gamma significantly reduced TER and increased epithelial permeability , effects prevented by ST / LA or BT . ^^^ CONCLUSIONS : Deleterious effects of TNF alpha and IFN gamma on epithelial function are prevented by probiotic , and to a lesser extent , commensal pretreatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We quantified the number and distribution of intestinal immune cells ( granulocytes , mast cells , CD4+ , CD8+ , CD25+ , IgA+ lymphocytes ) and the mucosal mRNA expression of cytokines ( IFN gamma , TNF alpha , TGF beta , IL 10 ) and antimicrobial peptides ( PR 39 , NK lysin , prepro defensin beta 1 , protegrins ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Secretion of IL 15 , interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) , and granzyme B into cell culture supernatants was assessed by ELISA . ^^^ Untreated CD IEL , characterised by higher IL 15Ralpha expression , showed increased proliferation , production of IFN gamma and TNF alpha , and perforin / granzyme dependent cytotoxicity , and a decreased propensity to apoptosis in response to IL 15 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation of CD1d restricted semiinvariant natural killer T cells by using the CD1d ligand alpha galactosylceramide ( alphaGalCer ) induces pregnancy loss in mice through an ill defined mechanism involving TNF , IFN gamma , and perforin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Results indicated significantly high levels of interferon gamma ( IFN gamma ) , interleukin ( IL ) 10 , and IL 6 during active KA , while tumor necrosis factor alpha ( TNF alpha ) , IL 2 , and IL 4 were minimal . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine polymorphisms ( TNFalpha 308 and 238 , IL 1beta 511 and +3954 , IL 1b RN + 2018 , IL 6 174 , IL 10 1082 , 819 and 592 , TGFbeta 1 + 29 and +74 , IFN gamma + 874 ) and HLA class 2 alleles were determined by sequence specific primer polymerase chain reaction or restriction fragment length polymorphism analysis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mesangial cells cultured from IRF 1 ( / ) mice produced significantly lower levels of nitric oxide and IL 12 but not TNF alpha when stimulated with LPS + IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
BAY 50 4798 induced approximately 5 fold lower mean levels of endogenous IL 2 than aldesleukin , and at least 50 % lower levels of proinflammatory cytokines , such as tumor necrosis fctor alpha ( TNF alpha ) , IL 1beta , IL 6 , and interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Administration of anti TNF alpha monoclonal antibody into TDM injected IFN gamma knock out mice decreased in granuloma formation , suggesting that development of pulmonary granuloma by mycobacterial glycolipids such as TDM is due to IFN gamma independent and TNF alpha dependent pathway . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although it is well known that OK 432 induces cytokines such as interferon ( IFN ) gamma and tumor necrosis factor ( TNF ) alpha , both of which are known to be potent NO inducers , we observed only a partial reduction of OK 432 induced NO production with the addition of anti IFN gamma and / or anti TNF alpha neutralizing antibodies . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation of cutaneous Vdelta 1 ( + ) gammadelta T cell lines induced secretion of large amounts of TNF alpha , IFN gamma , and the CCR 8 ligand CCL 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 23 also promoted the production of Th 1 cytokines such as IFN gamma , IL 12 and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here we demonstrate that MyD 88 increased the half life but not the synthesis of IFN gamma induced mRNA transcripts encoding tumor necrosis factor and IFN gamma inducible protein 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor presence resulted in dramatically elevated interleukin 2 ( IL 2 ) and decreased tumor necrosis factor alpha ( TNF alpha ) in supernatants of activated splenocytes , but had no significant effect on interferon gamma ( IFN gamma ) . ^^^ Administration of pXLG mEndo , radiation , or both modified the tumor induced aberrations in IL 2 and TNF alpha ; IFN gamma production was decreased by radiation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Ppg stimulation induced primarily IL 10 cytokine production , in addition to IFN gamma , IL 13 and TNF alpha secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increased immune cell populations in the spleens , as well as increased serum gamma interferon ( IFN gamma ) and tumor necrosis factor alpha levels were observed in infected mice treated with anti CD 40 and IL 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using beryllium specific CD4+ T cell lines derived from the bronchoalveolar lavage ( BAL ) fluid of CBD patients , we show that purified CD4+ T cells produced significant amounts of IFN gamma and TNF alpha upon exposure to beryllium in the absence of antigen presenting cells ( APC ) . ^^^ Restricting anti MHC class 2 mAb completely eliminated beryllium induced T cell proliferation during self presentation and significantly reduced IFN gamma and TNF alpha production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Co culture of TNF alpha pre stimulated A 549 cells with CD4+ T cells promoted CD 154 expression , CD4+ T cell proliferation and cytokine production , especially IFN gamma . ^^^ Monocyte derived TNF alpha in combination with IFN gamma and LPS markedly induced B 7 H2 expression in A 549 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study we elaborate on the immune response and mechanisms of persistence of mycobacteria in lesions by analysing , using immunohistochemistry , the expression of cytokines [ tumour necrosis factor alpha ( TNF alpha ) , interleukin 10 ( IL 10 ) , transforming growth factor beta ( TGF beta ) and interferon gamma ( IFN gamma ) ] , apoptotic cells and apoptosis related proteins [ Bcl 2 , Bax , Fas ligand ( FasL ) and Fas ] in the human tuberculous lymphadenitis lesions . ^^^ In epithelioid cells , the numbers of TNF alpha , IL 10 and TGF beta stained cells were higher than IFN gamma stained cells ( P < 0 . 01 ) . ^^^ More giant cells expressed IL 10 and TGF beta than expressed TNF alpha and IFN gamma ( P < 0 . 01 ) . ^^^ Apoptotic TB giant cells correlated positively with the expression of TNF alpha , IFN gamma and TGF beta , but not with the expression of IL 10 . ^^^ Compared with FB giant cells , there were fewer TB giant cells showing TNF alpha , IFN gamma , FasL , Fas expression or undergoing apoptosis ( P < 0 . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study , we exposed peripheral blood to estradiol , progesterone , prolactin and cortisol alone or in combination for 24 h before stimulation with T dependent ( phytohemagglutinin , PHA ) and independent activators ( lipopolysaccharide , LPS ) to study their immunomodulatory role in interleukin 12 ( IL 12 ) , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and IL 10 production in a whole blood model . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 4 reversed the impediment , attenuated TNF alpha production , and overcame blockage of IGF 1 production caused by IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Exogenous IFN gamma related reduction in free FA plasma levels and IFN gamma mRNA response , and up regulation of TNF alpha mRNA expression in CD 1 mice were associated with significant increase in adult worm burden and egg load . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both type 1 ( IFN gamma , tumor necrosis factor ( TNF ) alpha ) and type 2 ( IL 5 and IL 10 ) production by CD3 / CD28 activated CD 4 ( + ) T cells was further enhanced by IL 15 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After migration to asthmatic lung , these cells switch on effector cytokines and induce bronchial epithelial apoptosis with mainly IFN gamma , tumor necrosis factor ( TNF ) alpha , and Fas ligand . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Liver injury induced changes in the hepatic gene expression of hepcidin , hemojuvelin ( Hjv ) , several other proteins of iron metabolism , and several cytokines such as IL 1beta , IL 6 , TNF alpha , and IFN gamma . ^^^ In CCl ( 4 ) induced liver injury , IL 6 , IL 1beta , TNF alpha , and IFN gamma upregulation were found at the maximum 12 h after the administration of the toxin . ^^^ Treatment of isolated rat hepatocytes with IL 6 and , to a lesser extent , with IL 1beta but not with TNF alpha or IFN gamma dose dependently upregulated hepcidin and downregulated Hjv gene expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
During infection with S . enterica serovar Typhimurium ClpXP disrupted mutant , gamma interferon ( IFN gamma ) produced by CD4+ cells was up regulated on day 10 and tumor necrosis factor alpha ( TNF alpha ) produced by CD8+ cells was up regulated on day 30 after infection . ^^^ Typhimurium mutant on day 10 after infection , and IFN gamma , TNF alpha and transforming growth factor beta ( TGF beta ) were involved in maintenance of growth of this bacterium on day 30 after infection . ^^^ Typhimurium mutant , IFN gamma , TNF alpha , IL 10 and TGF beta may play different roles to maintain the persistent infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
As cell migration is dependent on extracellular matrix remodeling , involving proteolytic enzyme members of the matrix metalloproteinase ( MMPs ) family , we characterized the profile of expression of MMPs and their endogenous inhibitors ( TIMPs ) in rat NPCs , and evaluated the effects of TNF alpha , IFN gamma and IFN beta , a clinically proven modulator of brain inflammation , on the expression of these molecules . ^^^ NPCs ' gelatinase activities of MMP 2 and MMP 9 , as determined by zymography , were increased by TNF alpha , and to a lesser extent by IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This was accompanied by a reduced production of both IFN gamma and tumor necrosis factor alpha ( TNF alpha ) in the lungs . ^^^ The knockout mice appeared to have a delayed mycobacteria induced immune response with reduced expression of protective mediators , such as IFN gamma , TNF alpha , inducible nitric oxide synthase and regulated upon activation normal T cell sequence , during early infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment was highly effective with both doses , but at 40 mg / kg , almost complete parasite elimination was achieved . mRNA expression of gamma interferon ( IFN gamma ) and , to a lesser extent , tumor necrosis factor alpha ( TNF alpha ) and transforming growth factor beta ( TGF beta ) in spleen cells was up regulated in most animals of the untreated group . ^^^ Treatment with the lower dose of AMB HSA down regulated the mRNA expression of IFN gamma and TNF alpha , with no effect on the deactivating cytokine TGF beta . ^^^ In contrast , treatment with the higher dose ( 40 mg / kg ) of the formulation caused moderate up regulation of IFN gamma and TNF alpha and strong suppression of TGF beta . ^^^ Our results suggest that treatment of L . infantum infected Syrian hamsters with highly effective nontoxic doses of AMB HSA causes deactivation of the anti inflammatory cytokine TGF beta , which in turn results in up regulation of the Th 1 cytokines IFN gamma and TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma levels in culture supernatants did not reflect this response ; however , a decline in TNF alpha was observed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Sera from these individuals were measured for levels of IL 2 , IL 4 , IL 5 , IL 6 , IL 8 , IL 10 , IL 12 , IFN gamma , and TNF alpha by multiplexed microsphere cytokine immunoassay . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Different flow cytometric bead based assays were used to quantify some cytokines ( IL 1beta , IL 2 , IL 4 , IL 5 , IL 6 , IL 7 , IL 8 , IL 10 , IL 12 , IL 13 , IL 17 , G CSF , GM CSF , IFN gamma , MCP 1 , MIP 1beta , or TNF alpha ) in the culture media of oxysterol treated U 937 and THP 1 cells , and in the sera of healthy and atherosclerotic subjects . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The pathway of interferon gamma ( IFN gamma ) induced suppression in tumor necrosis factor related apoptosis inducing ligand ( TRAIL ) mediated apoptosis of fibroblast like synovial cells ( FLS ) was investigated . rTRAIL triggered FLS apoptosis in a type 2 cell death manner , whereas IFN gamma pretreatment significantly inhibited TRAIL mediated apoptosis . ^^^ The authors tried to determine the responsible molecules ; however , expression of TRAIL receptors ; pro caspase 3 / 8 / 9 ; Fas associated death domain protein ( FADD ) ; tumor necrosis factor receptor 1 associated death domain protein ( TRADD ) ; silencer of death domain ( SODD ) ; FLICE inhibitory protein ( FLIP ) ; and Bcl 2 , Bcl xL , and Bax in FLS was not modulated by IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There was a differential pattern of cytokine expression in pulp IF and serum with cytokines such as IL 1alpha , IL 1beta and TNF alpha locally produced , whereas others such as IFN gamma and IL 6 were produced systemically and probably spilled over to the pulp IF after LPS exposure . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Contrary to the parental strain , the auxotrofic mutant did not induce IL 1beta , IL 18 , TNF alpha , and IFN gamma in the ileum and plasma 24h after the infection , did not cause pathological changes in ileal epithelium and mesenteric lymph nodes or immunoreactivity of gp 91 phox and peroxynitrite and was not immunostained for GroEL stress protein . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
During the chronic stage of the infection , the bacterial load in the lungs of immune mice remained at a level 10 times lower than control mice , and this was associated with reduced numbers of CD4P ( +P ) and CD8P ( +P ) T cells , and the lower expression of protective ( IL 12 , IFN gamma ) , inflammatory ( TNF alpha ) , immunoregulatory ( GM CSF ) , and immunosuppressive ( IL 10 ) cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum concentrations of IL 1beta , IL 2 , IL 4 , IL 5 , IL 6 , IL 8 , IL 10 , IL 12p70 , TNF alpha and IFN gamma were concurrently measured by a new technique , the cytometric bead array ( CBA ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The possible involvement of cytokines such as tumour necrosis factor alpha ( TNF alpha ) , interleukin 1 beta ( IL 1 beta ) and interferon gamma ( IFN gamma ) that are suspected of causing pregnancy loss and miscarriage has been investigated in dams of mice subjected to hyperthermia . ^^^ Elevated levels of TNF alpha and IL 1 beta were observed in the amniotic fluid of dams subjected to hyperthermia during days 6 15 p . c . but IFN gamma levels remained unaltered . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Plasma and liver cytokines , tumor necrosis factor alpha ( TNF alpha ) , interleukin 6 , 1beta , 10 ( IL 6 , 1beta , 10 ) and interferon gamma ( IFN gamma ) , were measured by ELISA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
HMSC from multiple donor bones were treated with either activated T cell conditioned medium ( ACTTCM ) or physiological concentrations of the major inflammatory cytokines , TNF alpha , TGF beta , IFN gamma , and IL 17 ( TTII ) , individually or in combinations . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mice immunized with LPS+ sonicate developed a prominent innate response characterized by increased TNF alpha and IL 10 , as well as a strong antigen specific Th 1 response including , IFN gamma , IL 2 and high IgG2a serum titers . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There was a significant increase in tumor necrosis factor alpha ( TNF alpha ) but not IFN gamma secretion in NK cells treated either with anti CD 16 antibody alone or in combination with anti CD 94 antibodies . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Secretion of TNF , keratinocyte derived chemokine and monocyte chemoattractant protein 1 was attenuated in TLR 2 ( / ) mice , while IFN gamma was increased as in wild type mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
No changes were observed in IL 10 , TNF alpha and IFN gamma levels after the training program in either group . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We compared the suppression of LPS induced cytokine release ( monocyte chemotactic protein 1 [ MCP 1 ] , macrophage inflammatory protein [ MIP ] 1alpha , RANTES , tumor necrosis factor alpha , interleukin 1beta ( IL 1beta ) , IL 8 , IFN gamma , IL 6 , IL 10 , and granulocyte macrophage colony stimulating factor [ GM CSF ] ) by dexamethasone from PBMCs of patients with severe asthma ( n = 16 ) , patients with nonsevere asthma ( n = 19 ) , and normal volunteers ( n = 10 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of heat shock proteins Hsp 27 , Hsp 90 , and Hsp 70 and production of tumor necrosis factors ( TNF alpha , TNF beta ) , interferon gamma ( IFN gamma ) , interleukin 2 , 3 , 6 , and nitric oxide ( NO ) were studied under conditions of acute and chronic intoxication of animals with lipopolysaccharides . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
While TNF alpha and IFN gamma producing cells were numerous in stroma and epithelium , no IFN gamma producing cells were found on endothelium . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Particle mediated DNA vaccination of mice with a DNA plasmid encoding ICP 27 resulted in the induction of ICP 27 specific IFN gamma and TNF alpha production in Balb / c mice , but little protection to intranasal challenge with wild type HSV 2 . ^^^ The ICP27+LT mediated protection was correlated with a large increase in ICP 27 specific IFN gamma and TNF alpha production but cytokine specific monoclonal antibody treatment at the time of challenge showed that protection was mediated predominantly by IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inflammatory cytokines ( IFN gamma and TNF alpha ) were predominantly produced by heart infiltrating T cells upon stimulation with LMM peptides . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Splenocytes from infected C3H / HeJ mice produced almost no interleukin 1beta ( IL 1beta ) and tumor necrosis factor alpha ( TNF alpha ) upon ex vivo restimulation with B . pertussis compared to A / J mice and also showed a delayed gamma interferon ( IFN gamma ) production . ^^^ Functional Tlr 4 is essential for an efficient IL 1 beta , TNF alpha , and IFN gamma response ; efficient clearance of bacteria from the lung ; and reduced lung pathology . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A broad range of cytokine mRNAs were detected in 16 early nodules and 28 late stage ulcers , including those for the Th 1 cytokines tumor necrosis factor alpha ( TNF alpha ) and gamma interferon ( IFN gamma ) and the Th 2 cytokine interleukin 10 ( IL 10 ) . ^^^ Ten tissue samples containing granulomas showed high mRNA expression for IFN gamma , IL 1beta , IL 12p35 , IL 12p40 , IL 15 , and TNF alpha relative to 34 tissue samples without granulomas . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The results showed that laser treated mice presented reduction of footpad edema , faster cutaneous wound healing , confluent granuloma , diffuse and more loosely distributed immunolabeling for TNF alpha , enhanced labeling of IFN gamma and any P . brasiliensis form detected , whereas multiple viable fungi were seen in diffuse widespread granulomas obtained from non treated mice foot pad . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Multiplex analysis of broad cytokine protein production revealed a differential increase in the TH 1 and TH 2 associated cytokines : IL 2 , IL 4 , IL 8 , IL 10 , IL 12 , IFN gamma , TNF alpha and MCP 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This enhancement did not depend on interferon gamma ( IFN gamma ) , TNF alpha , or CD 40 ligand but did depend on cell to cell contact . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using enzyme linked immunospot ( ELISPOT ) assay and in situ hybridization techniques , immune responses in these animals were examined and showed elevated numbers of anti AChR IgG secreting B cells and AChR reactive interferon ( IFN ) gamma secreting cells , enhanced mRNA expression of the proinflammatory cytokines IFN gamma and tumour necrosis factor ( TNF ) alpha as Th 1 subset and the anti inflammatory cytokines interleukin ( IL ) 4 and IL 10 as a Th 2 subset , and transforming growth factor ( TGF ) beta as a Th 3 cytokine . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : In the current study we evaluated the in vitro production of the cytokines IFN gamma , TNF alpha , IL 5 and IL 10 by peripheral blood mononuclear cells ( PBMC ) from 15 disseminated leishmaniasis and 24 cutaneous leishmaniasis patients upon stimulation with L . braziliensis antigens genotyped as disseminated leishmaniasis or cutaneous leishmaniasis isolates . ^^^ The immune response of each patient evaluated by the two L . braziliensis antigens was assessed in a paired analysis in which we showed that L . braziliensis genotyped as disseminated leishmaniasis isolate was more potent than L . braziliensis genotyped as cutaneous leishmaniasis isolate in triggering IFN gamma and TNF alpha production in both diseases and IL 5 only in cutaneous leishmaniasis patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Circulating levels of interferon gamma ( IFN gamma ) and tumor necrosis factor ( TNF alpha ) were significantly elevated in HBO exposed as compared to HBO unexposed mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
At the same time , production of myelin oligodendrocyte glycoprotein Ag specific IFN gamma and TNF alpha by spleen cells was markedly suppressed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , in that inhibitors of the Akt pathway can sensitize otherwise resistant TBJ / Neuro 2a cells to apoptosis induced by IFN gamma plus TNF alpha , we hypothesized that bortezomib also could sensitize these cells to IFN gamma plus TNF alpha . ^^^ We demonstrate for the first time that bortezomib not only up regulates the expression of receptors for IFN gamma and TNF alpha on both TBJ neuroblastoma and EOMA endothelial cell lines , but also markedly enhances the sensitivity of these cells to apoptosis induced by IFN gamma plus TNF alpha in vitro . ^^^ Furthermore , bortezomib enhances the in vivo antitumor efficacy of IFN gamma / TNF alpha inducing cytokines , including both IL 2 and IL 12 in mice bearing well established primary and / or metastatic TBJ neuroblastoma tumors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 23 induces the differentiation of naive CD 4 ( + ) T cells into highly pathogenic helper T cells ( Th17 / Th ( IL 17 ) ) that produce IL 17 , IL 17F , IL 6 , and TNF alpha , but not IFN gamma and IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have shown that IL 23 dependent , pathogenic T cells produced IL 17 A , IL 17 F , IL 6 , and TNF but not IFN gamma or IL 4 . ^^^ Anti IL 23p19 treatment reduced the serum level of IL 17 as well as CNS expression of IFN gamma , IP 10 , IL 17 , IL 6 , and TNF mRNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We examined whether polymorphisms of IFN gamma , TNF alpha and IL 10 affect the susceptibility to and outcome of severe acute respiratory syndrome ( SARS ) . ^^^ We tested the polymorphisms of IFN gamma , TNF alpha and IL 10 for their associations with SARS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In mice injected intraperitoneally with bacterial lipopolysaccharide ( LPS ) , bladder prostasin mRNA expression was downregulated , whereas the expression of inducible nitric oxide synthase ( iNOS ) , cyclooxygenase 2 ( COX 2 ) , interferon gamma ( IFN gamma ) , TNF alpha , IL 1beta , and IL 6 was upregulated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We studied the expression and upregulation of costimulatory molecules ( CD 28 , CD40L / CD154 and CTLA 4 / CD152 ) and production of cytokines ( IL 2 , IL 4 , IL 6 , IL 10 , IFN gamma and TNF alpha ) in purified T lymphocytes from CVID patients stimulated with optimal doses of anti CD 3 or suboptimal doses of anti CD 3 and anti CD 28 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These hypotheses are discussed in view of more recent findings regarding the pathogenesis of yellow fever and regarding hepatic physiopathology , and a new hypothesis is proposed : the midzonal necrosis is consequence of action of combined factors mainly the direct cytopathic effect of YFV associated with a potent immune response in which CD4+ and CD8+ lymphocytes and the cytokines , especially TGF beta , but also TNF alpha and IFN gamma play an important role . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cultured HRPE cells were incubated with either IL 1 beta ( 0 . 2 20 ng / ml ) or tumor necrosis factor ( TNF ) alpha ( 0 . 2 20 ng / ml ) alone or in combination with interferon gamma ( IFN gamma ) ( 1000 U / ml ) . ^^^ HRPE cells were also incubated with : ( 1 ) media conditioned by activated human T lymphocytes ( CM ) , or ( 2 ) the same CM treated with neutralizing antibodies to IL 1 , TNF , and / or IFN gamma . ^^^ HRPE cells produced very high levels of IP 10 in response to either IL 1 beta / IFN gamma , TNF alpha / IFN gamma or CD 3 activated T lymphocyte CM . ^^^ Neutralizing antibodies to TNF and IFN gamma , but not to IL 1 , abrogated the ability of the CD 3 activated T lymphocytes CM to induce HRPE IP 10 ( p < . 001 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 23 can enhance the proliferation of memory T cells and the production of IFN gamma , IL 12 and TNF alpha from activated T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : We utilized a jacalin IgA capture assay to determine the IgA 1 titers in total saliva and reverse transcriptase polymerase chain reaction to detect mRNA for interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) in total saliva samples of 13 patients with chronic moderate periodontal disease and 10 with chronic severe periodontal disease . ^^^ Despite the small number of patients , IFN gamma expression had a trend association with severity of periodontitis and TNF alpha gene expression did not correlate with severity of periodontal disease . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We examined the pulmonary vascular permeability and production of proinflammatory cytokines , such as TNF alpha , IL 6 , IL 10 and IFN gamma , which play prominent roles in the pathogenesis of lupus like tissue injury , 6 h and 72 h after i . p . lipopolysaccharide ( LPS ; endotoxin ) injection in pristane primed chronic inflammation ICR mice characterized by a lupus like syndrome . ^^^ These results demonstrated that levels of serum IL 6 , IL 10 and IFN gamma and bronchoalveolar lavage ( BAL ) IL 6 and IFN gamma were remarkably increased 6 h in LPS exposed pristane primed mice compared with pristane primed controls , while pulmonary vascular permeability and levels of serum and BAL TNF alpha were not . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Following vaccination , T lymphocyte survival improved and cells recovered their ability to produce the Th 1 associated cytokines TNF and IFN gamma in response to anti CD 3 stimulation in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum levels of IL 10 , IL 12 and IFN gamma were increased in MCP 1 transgenic mice on day 6 following MCMV infection , while levels of IL 1beta and TNF alpha were undetectable . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increased uterine NK derived IFN gamma and TNF alpha in C57BL / 6J mice during early gestation . ^^^ It was demonstrated that the expression of IFN gamma and TNF alpha in uterine endometrium of pregnant mice are lower than those in liver , but they increase significantly during pregnancy . ^^^ They produced more IFN gamma and TNF alpha than other organ derived NK cells , and they also secreted minor amount of IL 4 and IL 5 . ^^^ The results indicated that the IFN gamma and TNF alpha produced by uNK cells ensured a successful pregnancy progress . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A variety of cytokines , such as IL 1beta , IL 2 , IL 4 , IL 5 , IL 6 , IL 8 , IL 10 , IFN gamma , TNF alpha , G CSF and MIP 1beta , were significantly elevated in ADEM . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CBD treatment also resulted in the significant reduction of plasma levels of the pro inflammatory cytokines , IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study investigated the immunological factors , such as neutrophils number , the level of myeloperoxidase and IL 12 , IL 10 , TNF alpha , IFN gamma , that additionally might correlate with increased susceptibility to Candida infections in cancer patients . ^^^ In the group of Candida colonized patients , the concentrations of IL 12 , TNF alpha and IFN gamma were significantly heightened when compared to control . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We recently found that the splenocytes from naive DBA / 1 and DBA / 2 mice are potently induced by SCG to produce interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and interleukin 12p70 ( IL 12p70 ) , and that GM CSF plays a key biologic role among these cytokines . ^^^ Cell cell contact involving intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 1 ( LFA 1 ) was an essential step for the induction of GM CSF and IFN gamma by SCG but not for the induction of TNF alpha or IL 12p70 by SCG . ^^^ GM CSF was required for the induction of TNF alpha by SCG , and in turn , TNF alpha enhanced the release of GM CSF and thereby augmented the induction of IL 12p70 and IFN gamma by SCG . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Single nucleotide polymorphisms were analysed for five cytokines : tumour necrosis factor alpha ( TNF alpha ) , transforming growth factor beta 1 ( TGF beta 1 ) , interferon gamma ( IFN gamma ) , interleukin 6 ( IL 6 ) and interleukin 10 ( IL 10 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Poly ( 1 : C ) treatment was associated with a rapid induction of inflammatory cytokines in the serum , including IL 6 , IL 10 , MCP 1 , TNF alpha , IFN alpha , and IFN gamma , and selective increases in the numbers of NK ( NK1 . 1 ( + ) CD11b ( + ) ) cells and Mvarphi ( NK1 . 1 ( ) CD11b ( + ) ) , but not NK T ( CD 3 ( + ) NK1 . 1 ( + ) ) cells . ^^^ Poly ( 1 : C ) treatment in TNF alpha , type 1 IFNR , IFN gamma , IL 6 , IL 12Rbeta2 , or IL 15 gene deficient mice revealed a reciprocal interaction and interdependence in the induction of these cytokines , where the absence of one cytokine impacted on the production of others . ^^^ Further , the adjuvant effects of poly ( 1 : C ) were dependent on the endogenous levels of type 1 IFNs , TNF alpha , IFN gamma , IL 12 , and IL 15 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
At the parameters exceeding the norm , the contents of TNF alpha , IL 6 and IFN gamma fell , on average , by 34 , 12 and 1 . 5 times , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : A dramatic decrease in the level of pro inflammatory cytokines TNF alpha , IL 6 , and IFN gamma was revealed : at 0 . 5 h after exposure of volunteers ( with the initial parameters exceeding the norm ) , the cytokine contents fell , on average , 34 , 12 , and 1 . 5 times . ^^^ The reduced concentrations of TNF alpha and IL 6 were preserved after four daily exposures , whereas levels of IFN gamma and IL 12 decreased five and 15 times . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Soon after virus elimination , we observed functional impairment of CTL isolated from the airways in their ability to produce IFN gamma and TNF alpha and to lyse target cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Similarly , administration of alphaCD 40 to both wild type and CD 40 / mice bearing Renca tumors resulted in substantial amounts of TNF alpha and MCP 1 in the serum , increased the number of total splenocytes and MHC class II+ CD11c+ leukocytes , and when combined with IFN gamma , inhibited the progression of established Renca tumors in vivo in both wild type and CD 40 / mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we investigate the role of IFN gamma and TNF alpha in the induction of fgl 2 expression and fgl 2 dependent hepatic apoptosis . ^^^ Infusion of IFN gamma in combination with TNF alpha through the portal vein of fgl2+ / + mice led to widespread hepatic apoptosis and fibrin deposition . ^^^ In vitro , IFN gamma and TNF alpha induced fgl 2 expression in a macrophage and endothelial cell specific manner . ^^^ In macrophages ( peritoneal and RAW 264 . 7 cells ) , IFN gamma , but not IFN alpha , LPS , TNF alpha , or IL 1 induced fgl 2 mRNA transcription and protein expression , while in endothelial cells TNF alpha , but not IFN gamma , induced fgl 2 transcription . ^^^ In addition , while TNF alpha enhanced IFN gamma induced macrophage fgl 2 transcription , IFN gamma also enhanced TNF alpha induced endothelial cell fgl 2 transcription . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Blood samples were analysed for C reactive protein , and cytokines IL 1beta , IL 2 , IL 4 , IL 6 , IL 8 , IL 10 , IL 12 , GM CSF , IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Overall , compared to C57BL / 6 mice , BALB / c mice demonstrated a significantly higher degree of lung tissue inflammatory cell infiltrate , BAL cellularity , and release of pro inflammatory cytokines ( TNF alpha , keratinocyte derived chemokine ( KC , a mouse homolog of human chemokine Gro alpha [ CXCL 1 ] , and IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We report that IFN gamma augments induction of TNFalpha by TLR ligands , immune complexes , and zymosan by suppressing IL 10 production and thereby interrupting Stat 3 mediated feedback inhibition . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The enhanced susceptibility of MIF / mice to T . cruzi was associated with reduced levels of proinflammatory cytokines , such as tumor necrosis factor alpha , interleukin 12 ( IL 12 ) , IL 18 , gamma interferon ( IFN gamma ) , and IL 1beta , in their sera and reduced production of IL 12 , IFN gamma , and IL 4 by spleen cells during the early phase of infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here , we demonstrate that protection by either protocol relies upon both gamma interferon ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) cytokines classically associated with type 1 cellular immunity . ^^^ In both protocols , abrogating IFN gamma or TNF alpha activity significantly decreases survival and increases the bacterial burden in pulmonary , splenic , and hepatic tissues . ^^^ We conclude that IFN gamma , TNF alpha , and NOS 2 , key elements of cellular immunity , perform critical protective functions during humoral defense against lethal pulmonary Y . pestis challenge . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
High levels of pro inflammatory mediators ( e . g . interleukin 1 [ IL 1 ] , tumor necrosis factor [ TNF ] , gamma interferon [ IFN gamma ] , high mobility group protein 1 [ HMGB 1 ] , macrophage migration inhibitory factor [ MIF ] ) produced locally and released into the blood stream initiate remote organ injury as a consequence of an organ cross talk . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
While the majority of LPSs induced cytokine release equipotently , a 1 , 000 fold more LPS from Pseudomonas aeruginosa or Vibrio cholerae was still less potent in inducing TNF , IL 1 beta , IL 10 and IFN gamma though it potently induced nanogram quantities IL 8 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both LPS and SA significantly ( p < 0 . 05 ) induced cytokine production IL 6 > TNF alpha > IL 1 beta > GM CSF > IL 10 > IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This was accompanied by a several hundred fold increase in serum levels of pro inflammatory cytokines such as IL 12 , IL 6 , TNF alpha , IFN gamma , as well as MCP 1 in HLA class 2 transgenic mice but not in C57BL / 6 mice ; CD 4 or CD 8 T cells independently contributed to the systemic cytokine response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This in vitro study examined whether hepatocytes can eliminate other cells via a CD 95 ligand ( CD95L or FasL ) / CD95 ( Fas ) mediated mechanism and whether this cytotoxic activity can be modulated by cytokines such as interferon gamma ( IFN gamma ) or tumor necrosis factor alpha ( TNF alpha ) . ^^^ Furthermore , exposure of cultured hepatocytes to IFN gamma or their stable transfection with IFN gamma cDNA or TNF alpha cDNA increased hepatocyte CD95L / CD95 mediated cell killing . ^^^ IFN gamma and , to a lesser extent , TNF alpha can enhance hepatocyte CD95L mediated cytotoxicity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Natural killer T ( NKT ) cells are recognized to be the key effector cells , and a variety of cytokines [ e . g . , interleukin 4 ( IL 4 ) , IL 5 , interferon gamma ( IFN gamma ) , and tumor necrosis factor alpha ( TNF alpha ) ] have been shown to play vital roles in Con A induced liver injury , whereas the role of IL 15 , a critical cytokine in the development and homeostasis of NKT cells , remains obscure . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mean concentrations of cytokines determined in the antral mucosa before treatment equaled : IL 2 53 . 1 ; IL 4 27 . 7 ; IL 5 39 . 4 ; IL 8 41 . 4 ; IL 10 33 . 0 ; IFN gamma 60 . 3 ; TNF alpha 27 . 7 pq / mg of protein . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lipopolysaccharide ( LPS ) , interferon gamma ( IFN gamma ) , interleukin ( IL ) 1beta , tumor necrosis factor alpha , IL 4 , IL 13 , IL 10 , IL 6 , IL 18 , and combinations thereof did not induce CCL 1 production in monocytes , and some of these signals stimulated production of reference chemokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The hypervirulence of the Delta cmaA 2 strain depended on host TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Judging by low levels of IFN gamma , TNF alpha and IL 6 in the treated mice , this effect can not be ascribed to Th 1 inflammation , but rather to a direct cytotoxic effect on the tumor cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , a small number of T cells expressing TNF alpha alone without concomitant IFN gamma or IL 4 expression were seen in the majority of subjects . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There were no significant differences in IL 5 , IFN gamma , or TNF alpha between the omalizumab and placebo groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
ICOS ( / ) CD 4 ( + ) T cells had no defect in expansion , but expressed significantly less Fas ligand and produced significantly lower levels of IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Despite virus replication and the expression of several chemokines and IL 6 in both regions being similar , the up regulation of MIP 1beta , TNF alpha , IFN gamma , and ICAM 1 and the loss of BBB integrity was more extensive in the cerebellum than in the cerebral cortex . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
They also displayed a defective ability to sustain TNF alpha and IFN gamma , but not IL 4 , production after in vitro stimulation , and the defect was restricted to innate immune cells . ^^^ Surprisingly , on the depletion of CD 14 ( + ) monocytes , this selective impairment of TNF alpha and IFN gamma production was restored to levels comparable to that observed in controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The generation of TNF alpha , IL 6 , IL 12 , and IFN gamma was investigated in the lung homogenates in the early periods after intranasal infection of mice with A / Leningrad / 134 / 57 ( H2N2 ) wild type virus and cold adapted attenuated vaccine viruses : A / Leningrad / 134 / 17157 ( H2N2 ) and A / Leningrad / 134 / 47 / 57 ( H2N2 ) . ^^^ Wild type virus induced substantially higher levels of proinflammatory cytokines : TNF alpha , IL 6 , IL 12 , and IFN gamma . ^^^ At the same time , the more attenuated strain A / Leningrad / 134 / 47 / 57 induced TNF alpha and IFN gamma in the quantities similar to those in the control animals . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the current study , we compared the expression of NKp 46 ( a natural killer cell marker ) , CD 3 ( a T cell marker ) , interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ; pro inflammatory cytokines ) and interleukin 10 ( IL 10 ; an anti inflammatory cytokine ) mRNA in the liver of naive HIV / HCV coinfected patients ( group one , n=14 ) , coinfected patients treated with antiretroviral therapy ( group two , n=23 ) and naive HCV mono infected patients ( group three , n=24 ) . ^^^ Interestingly , when compared to groups two and three , group one showed significantly higher intrahepatic mRNA levels for CD 3 , IFN gamma and TNF alpha , whereas the expression of NKp 46 and IL 10 were comparable in all three groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : There is a highly significant increase of TNF 857 TT and CT genotypes ( p = 0 . 002 ) among patients with respect to controls and a significant decrease of IFN gamma low producers ( A / A ) ( p = 0 . 04 ) among patients with respect to controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The regression of a canine Langerhans cell tumour is associated with increased expression of IL 2 , TNF alpha , IFN gamma and iNOS mRNA . ^^^ Furthermore , the expression of interleukin ( IL 2 ) , IL 12 ( p 40 ) , tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , IL 10 and transforming growth factor beta ( TGF beta ) as well as inducible nitric oxid synthase ( iNOS ) mRNA was determined by reverse transcription polymerase chain reaction ( RT PCR ) . ^^^ The latter was significantly correlated to up regulation of IL 2 , TNF alpha , IFN gamma and iNOS mRNA expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The aim of the study is to analyse the expression of pro inflammatory cytokines IL 1beta , TNF alpha , IFN gamma and the chemokine CXCL 8 ( IL 8 ) in liver tissue and their expression and secretion in PBMC of patients with chronic hepatitis C ( CHC ) , in response to pentoxyfilline ( PTX ) . ^^^ Pentoxyfilline resulted in decreased expression of mRNA of liver IL 1beta , TNF alpha and IFN gamma : 144 . 2 versus 83 . 5 molecules of IL 1beta ( P < 0 . 05 ) , TNF alpha 194 . 3 versus 17 . 6 molecules ( P = 0 . 03 ) and IFN gamma 26 . 1 versus 0 . 5 molecules ( P = 0 . 04 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : We used intracellular cytokine staining and flow cytometry to determine the cytokine expression of splenic CD 4 ( + ) T cells for interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) , interleukin 4 ( IL 4 ) and IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of Tregs with HSP 60 , or its peptide p 277 , before anti CD 3 activation significantly enhanced the ability of relatively low concentrations of the Tregs to downregulate CD4+CD25 or CD8+ target T cells , detected as inhibition of target T cell proliferation and IFN gamma and TNF alpha secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Peripheral blood T cells were activated by anti CD 3 monoclonal antibodies in the absence and presence of halofuginone and assessed for nuclear factor ( NF ) kappaB activity , production of tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) , T cell apoptosis , chemotaxis , and phosphorylation of p 38 mitogen activated protein kinase ( MAPK ) . ^^^ In addition , 40 ng / ml halofuginone inhibited secretion of TNF alpha , IFN gamma , interleukin ( IL ) 4 , IL 13 , and TGF beta ( P < 0 . 005 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The ratios of IL 4 : IFN gamma and IL 10 : TNF alpha were significantly higher in women . ^^^ The higher IL 4 : IFN gamma and IL 10 : TNF alpha ratios seen in women indicate that postmenopausal women have T helper type 2 ( Th 2 ) directed reactivity with impaired inflammatory responses which might inhibit the elimination of spirochetes . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cell free tear and culture supernatants were assayed for IL 1beta , IL 2 , IL 4 , IL 5 , IL 6 , IL 8 , IL 10 , IL 12 , IL 13 , IFN gamma , TNF alpha , eotaxin , MCP 1 and RANTES using multiplex bead analysis . ^^^ In addition IL 4 , IFN gamma , and IL 10 were elevated in SAC and VKC , while eotaxin and TNF alpha were only increased in VKC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A biochemical evaluation for a battery of cytokines was undertaken ( IL 1beta , IL 1ra , IL 2 , IL 2R , IL 4 , IL 5 , IL 6 , IL 7 , IL 8 , IL 10 , IL 12p40 , IL 13 , IL 15 , IL 17 , TNF alpha , IFN alpha , IFN gamma , GM CSF , MIP 1alpha , MIP 1beta , IP 10 , MIG , Eotaxin , RANTES , and MCP 1 , and neuropeptides ) using high resolution multiplex bead immunoassays and enzyme linked immunosorbent assay ( ELISA ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Exposure to LPS leads to activation of cytokines , including TNF alpha , IL 1beta , IL 6 , and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The proinflammatory cytokines interferon gamma ( IFN gamma ) and tumour necrosis factor ( TNF ) are implicated in EAE pathogenesis . ^^^ Signals through the type 1 TNF receptor ( TNFR 1 ) are required for severe EAE to develop , whereas deficiency in IFN gamma or its receptor result in more severe EAE . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This alteration in ligand specificity was demonstrated both in TLR transfected HEK cells , and also in human PBMC , with a corresponding change in cytokine production away from IFN alpha secretion by TLR 7 expressing plasmacytoid DC and toward IL 12 , TNF alpha and IFN gamma secretion by TLR 8 expressing monocytes and NK cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition to TNF , protein arrays showed IL 6 , IL 10 , MIP 1alpha , MIP 2 , and RANTES were also secreted following treatment with 10 microg / ml DMXAA alone , and IL 4 , IFN gamma , MCP 5 , and TIMP 1 were additionally induced using a higher dose of 300 microg / ml DMXAA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Values from immunodepressed mice ( e . g . alpha / beta T cells , TNF , IFN gamma and iNOs knock out strains ) , with accelerated bacillary growth rate , confirmed this fact . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Significantly , self specific CD 8 T cells are also more efficient in the production of IFN gamma and TNF alpha , thus providing more cytokine dependent protection against bacterial infection when compared with NK cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
According to the last literature data , three routes of activation of eosinophil can be distinguished : Th 2 route connected with cytokines : IL 5 , IL 4 and IL 13 , Th 1 route connected with cytokines : IFN gamma 11 12 , GM CSF , as well as internal route connected with IL 1 , TNF , GM CSF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In order to quantify in vivo the mRNAs of cytokines which play important roles in leptospirosis , we have developed quantitative real time PCR assays for interleukin 2 ( IL 2 ) , IL 4 , IL 10 , IL 12p40 , tumor necrosis factor alpha ( TNF alpha ) , gamma interferon ( IFN gamma ) , transforming growth factor beta , and two housekeeping genes ( encoding beta actin and hypoxanthine phosphoribosyltransferase ) . ^^^ In this kinetic study , there was pronounced expression of Th 1 cytokine mRNA ( TNF alpha , IFN gamma , and IL 12 ) , with transcripts being detected as early as 1 h postinfection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of T helper ( Th ) 1 cytokines tumor necrosis factor alpha ( TNF alpha ) , interleukin 12 ( IL 12 ) , and interferon gamma ( IFN gamma ) were dramatically elevated after ConA in CDD fed mice compared with control fed mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Levels of transcripts encoding for pro and anti inflammatory markers ( tnf alpha , ifn gamma , il 10 , t bet , gata 3 ) as well as for TGF beta 1 signaling mediators ( smad 3 , smad 4 , smad 7 ) were followed by real time PCR in a prospective study enrolling 6 AGMs and 6 MACs . ^^^ RESULTS : During primary SIVmac infection , up regulations of tnf alpha , ifn gamma and t bet responses ( days 1 16 p . i . ) were stronger whereas il 10 response was delayed ( 4th week p . i . ) compared to SIVagm infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore CD4+ suxamethonium reactive T cell lines were IFN gamma positive and synthesized high levels of IFN gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The production of IFN gamma was lowest in the patient group , whereas IL 12 , IL 15 and TNF alpha were produced in higher levels in patients than in the donors and survivors . ^^^ Furthermore , the patients at later stages of NPC had lower gammadelta CTL activity but higher NK cytotoxicity towards NPC targets , with higher production of IL 12 , IL 15 and TNF alpha but lower production of IFN gamma than in patients at earlier stages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When astrocytes were treated with IL 1beta , TNF alpha or IFN gamma , TRAIL was induced in cultured fetal astrocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , during infection , splenocytes and purified T cells lost their ability to proliferate in response to concanavalin A stimulation more rapidly in the Mtb+27kDa infected mice , which was accompanied by high IFN gamma and NO production , but low TNF alpha secretion levels . ^^^ Addition of L NMMA , anti IFN gamma and anti TNF alpha antibodies restored in vitro proliferative responses of T cells from Mtb+27kDa infected mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CD8+ T cells are important contributors to viral clearance , utilizing contact dependent effector functions ( perforin and CD95L ) as well as IFN gamma and TNF alpha . ^^^ IFN gamma and TNF alpha are primary perpetrators of T cell mediated lung injury , with TNF as the major contributor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , recruitment of monocytes to TNF alpha + interferon gamma ( IFN gamma ) and of PMN to all stimuli tested was not affected by VEGF . ^^^ With the potent TNF alpha + IFN gamma stimulus , in contrast to bFGF , VEGF did not enhance E selectin or ICAM 1 expression . bFGF , but not VEGF , increased the chemotactic activity for PMN in TNF alpha + IFN gamma inflamed sites by 54 % . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The engagement of CD 137 ( 4 1BB ) , an inducible T cell costimulatory receptor and member of the TNF receptor superfamily , by agonistic Abs can promote strong tumor and viral immunity mediated by CD 8 ( + ) T cells and stimulate IFN gamma production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Decreased function following rotenone incubation was not restricted to naive cells , as effector and memory CD8+ T cells isolated directly ex vivo from lymphocytic choriomeningitis virus infected mice displayed decreased production of IFN gamma and TNF alpha production after peptide stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Prostate specific autoantibodies as well as elevated levels of NO , TNF alpha , and IFN gamma were also detected in their seminal plasma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increased levels of IL 1beta , IL 4 , IL 6 and IL 10 and low concentrations of TNF alpha and IFN gamma were observed in both CSF and serum . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vivo treatment with saporin conjugated anti CD11b mAb decreased CD 4 ( + ) T cell infiltration in the colon and suppressed interferon gamma ( IFN gamma ) and TNF alpha production by lamina propria CD 4 ( + ) T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Then , we investigated whether a combination of TNF alpha , IL 1beta and IFN gamma may affect the phenotype of murine NSCs in vitro . ^^^ RESULTS : We found that TNF alpha , IL 1beta and IFN gamma , which are absent in the normal SC , are all expressed in the injured SC and the expression of these cytokines follows a timely tuned fashion with IFN gamma being detectable as long as 4 weeks after injury . ^^^ In culture , exposure of proliferating NSCs to a combination of TNF alpha , IL 1beta and IFN gamma was per se sufficient to induce the astrocytic differentiation of these cells even in the absence of serum . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The different virus specific effector and memory sets are compared by phenotypic [ CD62L , interleukin 7 receptor alpha ( IL 7Ralpha ) , and IL 15Rbeta expression ] and functional [ interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and IL 2 production ] analyses . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The CpG sequences present in plasmid DNA are immunostimulatory , i . e . they induce pro inflammatory cytokines , such as IL 12 , TNF alpha , IFN gamma and IFN alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PBMC were tested for secretion of tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , interleukin 5 ( IL 5 ) and IL 10 in response to complex ( whole cells , culture filtrate and cell walls ) , single secreted ( Ag85B , ESAT 6 , MPT 64 , PstS and MPT 70 ) and single cytosolic ( DnaK , GroES and GroEL ) antigens of Mycobacterium tuberculosis . ^^^ In the absence of antigens , detectable concentrations of TNF alpha , IFN gamma and IL 10 were secreted by PBMC of both donor groups , but the concentrations of only IL 10 were significantly higher ( P=0 . 015 ) in TB patients than in healthy subjects . ^^^ In the presence of complex antigens , PBMC secreted IFN gamma and TNF alpha in response to all three preparations , whereas IL 10 was secreted in response to whole cells and cell walls only . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increased T cell production of TNF is induced by estrogen deficiency via a complex mechanism mediated by antigen presenting cells and involving the cytokines IFN gamma , IL 7 , and TGF beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of the pro inflammatory Th 1 cytokines : IFN gamma , TNF alpha in splenic lymphocytes was altered toward a beneficial Th 2 pattern . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Febrile PFAPA attacks led to a significant increase in IL 6 and IFN gamma serum concentrations compared to symptom free periods and to controls , with IL 1beta , TNF alpha and IL 12p70 levels being significantly higher than in controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cellular immune responses toward a Th 1 subset mediated by IFN gamma and TNF alpha predominate in asymptomatic dogs exhibiting apparent resistance to visceral leishmaniasis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Knockout mice used in this study included interleukin 4 ( IL 4 ) , IL 10 , IL 12p40 , interferon gamma ( IFN gamma ) , and tumour necrosis factor ( TNF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Uptake of TcSnC is altered by gram negative infection , possibly due to the endotoxin component lipopolysaccharide ( LPS ) or to cytokines released during infection ( e . g . , TNF alpha and IFN gamma ) . ^^^ METHODS : Whole blood from six healthy volunteers was incubated with LPS , TNF alpha , IFN gamma , GM CSF or G CSF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We find that a recently described analogue , alpha C galactosylceramide ( alpha C GalCer ) , more potently induces these innate and adaptive immune responses in mice . alpha C GalCer acts as a more effective trigger for IL 12 and IFN gamma production , although it minimally elicits IL 4 and TNF alpha release into the serum . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In analogy with TLR ligands , tumor necrosis factor alpha ( TNF alpha ) or interleukin 1beta ( IL 1beta ) , in combination with IFN gamma , synergistically induced CXCL 9 and CXCL 11 in HMVEC and human fibroblasts , two fundamental cell types delineating the joint cavity . ^^^ Etanercept , a humanized soluble recombinant p 75 TNF receptor / IgG ( 1 ) Fc fusionprotein , neutralized synergistic CXCL 9 production induced by TNF alpha plus IFN gamma , but not synergy between IFN gamma and the TLR ligands PGN or LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We estimated the percentage of CD4+ lymphocytes synthesizing IFN gamma and IL 4 in the peripheral blood and measured the concentrations of IL 1 , IL 6 , and TNF alpha in lochia of patients with postpartum endometritis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neutralization of IFN gamma , TNF alpha , or both did not induce reactivation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study on peripheral blood leukocytes of healthy male subjects , we scrutinized the influence of prior sex hormones ( for 24 h ) with and without later addition of cortisol ( for another 24 h ) on stimulated secretion of TNF , IL 2 , IL 4 , IL 6 , IL 10 , and interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pro inflammatory cytokines , such as tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , and interleukin 6 ( IL 6 ) , are critical mediators in this experimental model . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 4 and TGF beta 1 downregulate TNF alpha and IFN gamma induced TARC production in the human KC cell line , HaCaT cells , while IL 4 upregulates , and IFN gamma downregulates TARC production by mouse LC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In recipients of C57BL / 6J CD4+ cells but not recipients of T bet KO cells , gastritis was associated with a delayed type hypersensitivity response to H . pylori antigen and elevated gastric and serum IFN gamma , interleukin 6 , and tumor necrosis factor alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We further demonstrate that the levels of the proinflammatory cytokines gamma interferon ( IFN gamma ) and interleukin 1beta ( IL 1beta ) in the serum were dramatically decreased in Per 2 / mice following LPS challenge , while production of tumor necrosis factor alpha , IL 6 , and IL 10 was approximately normal , compared to that in control wild type mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Because the transcription factor class 2 transactivator ( CIITA ) regulates expression of MHC class 2 antigens , we evaluated influences of CIITA and MHC class 2 antigens on suppression of colony formation by murine bone marrow HPC in response to HF , LF , CC , and CXC chemokines , TNF alpha , and IFN gamma . ^^^ MATERIALS AND METHODS : HF , LF , CCL3 / MIP 1alpha , CXCL5 / ENA 78 , CXCL8 / IL 8 , CCL5 / RANTES , TNF alpha , and IFN gamma were assessed for effects on colony formation by bone marrow HPC ( colony forming unit granulocyte macrophage , burst forming unit erythroid , and colony forming unit multipotential ) stimulated in vitro by combinations of growth factors including erythropoietin , stem cell factor , pokeweed mitogen mouse spleen cell conditioned medium , and hemin . ^^^ RESULTS : Multiple growth factor stimulated colony formation by control bone marrow HPC was significantly suppressed by HF , LF , CCL 3 , CXCL 5 , CXCL 8 , TNF alpha , and IFN gamma , but not by CCL 5 . ^^^ However , HPC from CIITA / and MHC class 2 antigen / mouse marrow was insensitive to inhibition by HF , LF , CCL 3 , CXCL 5 , CXCL 8 , and CCL 5 ; these HPC were inhibited by TNF alpha and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Simvastatin inhibited IFN gamma , TNF alpha , and IL 2 secretion , as well as the expression of T bet , a transcription factor that regulates Th 1 cell differentiation . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Blood samples were collected at first infection symptom and 2 , 5 , 12 , and 24 weeks later , and production of IL 4 , IL 10 , IL 12 , IFN gamma , TNFalpha , VLA 4 , LFA 1 , MMP 9 , and MMP 2 were measured after infectious antigens ( Ag ) stimulation . ^^^ Numbers of IFN gamma , TNFalpha , and IL 12 secreting cells , serum concentrations of MMP 9 , and expression of VLA 4 and LFA 1 after PBMC viral or bacterial Ag stimulation were higher in samples collected during exacerbations associated to SI . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Surprisingly , the decidual levels of the Th 1 cytokines IFN gamma and TNF alpha were not diminished after therapy . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The maximum increase in IL 6 , IL 10 , TNF alpha , IFN gamma in the blood was observed in 4 hours after administration of immunomodulators ( p < 0 . 001 ) . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Real time RT PCR was developed for quantification of mRNA encoding interleukin ( IL ) 2 , IL 4 , IL 5 , IL 6 , IL 10 , IL 12 ( p 35 and p 40 ) , IL 18 , tumour necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) and transforming growth factor beta ( TGF beta ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
KEY RESULTS : Oral administration of TFDG ( 5 mg kg ( 1 ) daily i . g . ) significantly improved TNBS induced colitis associated with decreased mRNA and protein levels of TNF alpha , IL 12 , IFN gamma and iNOS in colonic mucosa . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cells of horses with EPM mainly had expression of genes for IL 6 , IL 8 , IL 10 , TNF alpha , IFN gamma , and TGF beta . ^^^ Cells from horses with spinal cord trauma had expression mainly for IL 6 ; IFN gamma ; TGF beta ; and less frequently , IL 2 , IL 10 , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Intralesional TNF production was lower in mice of the treated groups , whereas interleukin 18 ( IL 18 ) and interferon gamma ( IFN gamma ) mRNA were not affected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neither IFN gamma nor TNF alpha alone had a noticeable effect on L . monocytogenes internalization , but a mixture of both cytokines resulted in decreased ( P < 0 . 01 ) internalization . ^^^ Thus , TNF alpha and IFN gamma acted synergistically to shed syndecan 1 ectodomains from HT 29 enterocytes , and shedding was associated with decreased internalization of two pathogenic bacteria , suggesting that syndecan 1 shedding may modulate the pathogenesis of specific microbes . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CADO strongly inhibited cytotoxic activity of LAK cells and attenuated the production of IFN gamma , granulocyte macrophage colony stimulating factor , tumor necrosis factor alpha , and macrophage inflammatory protein 1alpha by LAK cells stimulated by cross linking of the Ly49D receptor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Studies showed that LF elevates the number and increases the activity of T and B lymphocytes and NK cells , stimulates the release of a number of cytokines ( IL 1 , 6 , 8 , 18 , IFN gamma , TNF alpha ) , increases phagocytic activity and cytotoxicity of monocytes / macrophages , accelerates the maturation of T and B cells , and elevates the expression of several types of cellular receptors , such as CD 4 , zeta chain of the CD 3 complex , LFA 1 , CD 11 , ICAM 1 , and selectin P . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The loss was not induced by up regulation of the inflammatory cytokines TNF or IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , TNF alpha significantly increased the invasiveness of CB HMC across Matrigel coated membranes while the addition of IFN gamma , rTIMP 1 , or pharmacological MMP inhibitors significantly reduced this process . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After tumor necrosis factor alpha ( TNF alpha ) and IFN gamma stimulation , these proteins showed colocalization in the oligodendroglial process . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We evaluated the immunological changes induced by oral administration of OM 89 in 12 RA patients ( polyclonal T cell reactivity to PHA , T cell precursor frequencies specific for OM 89 and Tetanus toxoid ( TT ) , a control antigen and the release of Th 1 ( IFN gamma , TNF alpha ) , Th 2 ( IL 4 ) and T regulatory 1 cell ( Tr 1 ) ( IL 10 ) cytokines in the supernatants of PBMC cultures . ^^^ OM 89 induced a strong production of IL 10 , a significant decrease in IL 4 production while TNF alpha and IFN gamma production tended to decrease during the study . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment with interferon alpha ( IFN alpha ) , IFN gamma , interleukin 3 ( IL 3 ) , IL 6 or tumor necrosis factor alpha ( TNF alpha ) did not alter expression of the TIMP genes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These CTL mediated major histocompatibility complex ( MHC ) class 1 restricted tumour cell lysis , production of interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) , and expression of Fas ligand ( FasL ) and TNF related apoptosis inducing ligand ( TRAIL ) in response to C 15 cells . ^^^ The cytokines IFN gamma and TNF alpha synergistically enhanced surface expression of Fas , TRAIL receptor , MHC class 1 and class 2 on C 15 cells that increased the sensitivity of tumour cells to CTL lysis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In order to analyzed whether during therapy could be observed a change of cellular immune response pattern , we evaluated the production of IL 10 , TNF alpha and IFN gamma , as well as proliferation of peripheral blood mononuclear cell ( PBMC ) from patients in different periods of chemotherapy treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study , we demonstrate that the NK cells from simian immunodeficiency virus ( SIV ) infected rhesus monkeys are significantly impaired in their ability to secrete IFN gamma , TNF alpha , and IL 2 , while NK cell function in SIV infected long term non progressor monkeys is similar to that of normal monkeys . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , the addition of monoclonal antibodies specific to TNF alpha and IFN gamma to the blood cultures did not alter mycobacterial growth indicating the role of other mechanisms / factors in restricting the growth of M . tuberculosis in whole blood cultures . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NK like T cells display an activated phenotype and secrete various cytokines , including IFN gamma , TNF alpha , IL 5 , IL 13 and MIP 1beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Elevations in IL 10 , TNF alpha , and IFN gamma from the earliest point of HIV Type 1 infection . ^^^ Perturbations of plasma IL 10 , IL 12 , IL 13 , TNF alpha , and IFN gamma were measured longitudinally in HIV 1 seroconverting plasma donors and were compared to subjects with symptomatic primary HIV 1 infection . ^^^ IL 10 , TNF alpha , and IFN gamma rapidly rose in acute HIV 1 infection , while IL 13 predominated in acute HCV . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon ( IFN ) gamma ( + ) cell percentage and interleukin ( IL ) 4 ( + ) / tumor necrosis factor ( TNF ) alpha ( + ) ratio of Th cells in MS groups were increased , while IL 4 ( + ) cell percentage , IL 4 ( + ) / IFN gamma ( + ) and IL 4 ( + ) / TNF alpha ( + ) ratios of Tc cells in MS groups were decreased as compared with those in IA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation of patients ' cells with M . tb sonicate induced significantly less interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) and interleukin 10 ( IL 10 ) but more transforming growth factor beta ( TGF beta ) than controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To gain insight into the mode of action at the molecular level , gene expression analyses for the inducible nitric oxide synthase and the cytokines interleukin ( IL ) 1 , IL 12 , IL 18 , tumour necrosis factor ( TNF ) alpha , interferon ( IFN ) alpha , and IFN gamma , were performed using reverse transcription polymerase chain reaction ( RT PCR ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Along with reductions in serum TNF alpha and IFN gamma levels , there was also an increase in IL 4 and IL 10 transcript levels in lymph nodes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In in vitro studies , proinflammatory molecules such as LPS , lipoteichoic acid , dsRNA , TNF alpha , and IFN gamma can induce HMGB 1 release from macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increased TNF alpha and IFN gamma production of kidney infiltration CD3+ T cells in IRI mice but not sham operated mice was found . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
During infection , IEL increased production of gamma interferon ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) and decreased transforming growth factor beta ( TGF beta ) production . ^^^ Addition of IFN gamma and TNF alpha or supernatants obtained from cultured IEL from E . vermiformis infected mice reduced transepithelial electrical resistance ( TER ) in a confluent CMT 93 cell monolayer , a murine intestine derived epithelial line , but antibodies against these cytokines suppressed the decline of TER . ^^^ Moreover , TGF beta attenuated the damage of epithelial monolayer and changes in TER caused by IFN gamma and TNF alpha . ^^^ The expression of junctional molecules by EC was decreased when IEL produced a high level of IFN gamma and TNF alpha and a low level of TGF beta in E . vermiformis infected mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Plasma levels of IL 1beta , TNF alpha , IL 8 , IFN gamma , IL 1ra and TNF BP 1 , as well as stress hormones ( cortisol and prolactin ) were measured . ^^^ As for pro and anti inflammatory cytokine balance , the overall end result was a rise at 24 hr postoperatively , in the level of TNF alpha ( a key pro inflammatory cytokine ) and IFN gamma , as well as both anti inflammatory cytokines ( IL Ira & sTNF R 1 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines ( interferon gamma ( IFN gamma ) , interleukin [ IL ] 2 , IL 4 , IL 10 , IL 12 , IL 15 , and tumor necrosis factor alpha ( TNF alpha ) ) were measured by enzyme linked immunosorbent assay and compared between the uveitis groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Peripheral blood CD4+ T cell phenotype and activation status were evaluated by flow cytometry at 0 , 2 , and 8 weeks after treatment for expression of CD 69 , CD62L , chemokine receptors ( CCR 4 , CXCR 3 , and CCR 5 ) , and intracellular cytokines ( TNF alpha , IFN gamma , and IL 10 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A system of cytokines was studied by interleukine level ( IL 1 alpha , IL 1 beta , TNF alpha , IL 8 ) and IFN gamma content in cervical mucus . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha and double stranded RNA ( dsRNA ; TLR 3 ligand ) were potent inducers of TLR 2 and TLR 3 mRNA expression , and both stimuli had additive or synergistic effects with IFN gamma on TLR 2 and TLR 4 , but not TLR 3 , mRNA expression . ^^^ However , dexamethasone potentiated TLR 2 expression induced by combined IFN gamma and TNF alpha stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , when the glial cells are treated with IFN beta before coculture with T cells , the effector functions of T cells are impaired as IFN gamma , TNF alpha , and NO productions are decreased . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Whereas DCs from RR MS induced higher levels of Th 1 ( IFN gamma , TNF alpha ) and Th 2 ( IL 4 , IL 13 ) cytokines compared with controls , DCs from SP MS only induced a polarized Th 1 response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found that oh ( 8 ) dG reduces LPS induced reactive oxygen species ( ROS ) production , STAT 3 activation , and ICAM 1 expression . oh ( 8 ) dG also suppresses pro inflammatory cytokines , such as TNF alpha , IL 6 and IFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
L . casei strain Shirota stimulated PBMNC to secrete interleukin 12 ( IL 12 ) , gamma interferon ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The data revealed mixed T helper cell type 1 ( Th 1 ) and T helper cell type 2 ( Th 2 ) responses , as reflected by elevated expression of interferon ( IFN ) gamma , tumor necrosis factor ( TNF ) alpha , transforming growth factor ( TGF ) beta , interleukin ( IL ) 10 , IL 6 , and IL 4 mRNA , with minimal expression of IFN gamma receptor 1 ( IFN gamma R 1 ) mRNA in PKDL lesions , compared with that in normal skin tissue . ^^^ In comparison with those in KA lesions , mRNA levels for IFN gamma , TNF alpha , and IL 6 were found to be significantly elevated in PKDL lesions , implying that these cytokines play an important role in PKDL pathogenesis . ^^^ In the presence of elevated levels of IFN gamma and TNF alpha , interference with type 1 effector activity in PKDL may be due to minimal expression of the IFN gamma R 1 gene or the simultaneous presence of elevated levels of IL 10 , IL 6 , and TGF beta , which have counteracting effects . ^^^ Interferon ( IFN ) gamma , tumor necrosis factor alpha , interleukin 6 , and IFN gamma receptor 1 are the major immunological determinants associated with post kala azar dermal leishmaniasis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the horses with epm the pro inflammatory Th 1 cytokines ( IL 8 , TNF alpha and IFN gamma ) were commonly expressed but the anti inflammatory Th 2 cytokines ( IL 4 , IL 6 AND IL 10 ) were absent or rare . ^^^ In the horses with ehv 1 the proinflammatory cytokine IL 8 was commonly expressed , but IL 10 and IFN gamma were not , and TNF alpha was rare . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , CpG ODN enhanced the production of TNF alpha and Th 1 mediated cytokines , including IFN gamma and IL 2 compared with JE vaccine alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Longitudinally , HIV specific T cells in this individual were able to secrete IFN gamma and tumor necrosis factor alpha , as well as proliferate and degranulate in response to their cognate antigenic peptides up to 5 years postinfection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The results indicate no change in the cytokine expression ( Il 10 , TGF beta , IFN gamma , TNF alpha , MCP 1 ) in Peyer ' s patches and in mesenteric lymph nodes , and no modification in the uptake of yeast cells by Peyer ' s patches . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Patient BFU Es increased significantly following in vitro treatment with tumor necrosis factor alpha ( TNF alpha ) and / or interferon gamma ( IFN gamma ) neutralizing antibodies . ^^^ Local TNF alpha and IFN gamma production was higher in anemic than in nonanemic patients . ^^^ CONCLUSION : Impaired BM erythropoiesis in CIN patients is probably the result of increased local production of TNF alpha and IFN gamma that induce apoptosis , cell growth inhibition , and downregulation of EPOR expression on erythroid cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
HO 1 ( + / ) mice revealed enhanced neutrophil infiltration and proinflammatory cytokine ( TNF alpha , IL 6 , and IFN gamma ) induction , as well as an increase of intrahepatic apoptotic TUNEL ( + ) cells with enhanced expression of proapoptotic genes ( Bax / cleaved caspase 3 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Unlike GILT induction in response to IFN gamma treatment , induction by bacteria is dependent on new protein synthesis , NF kappaB signaling , and secretion of the inflammatory cytokines TNF and IL 1beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , DN Treg cells have been shown to express several molecules uncommon to other Treg cell subsets , such as IFN gamma , TNF alpha , Ly6A , FcRgamma , and CXCR 5 , which may contribute to their unique regulatory ability . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here we report that production of beta interferon ( IFN beta ) but not production of IFN alpha , IFN gamma , or tumor necrosis factor alpha ( TNF alpha ) strongly correlates with innate protection against HSV 2 . ^^^ There was no correlation between levels of TNF alpha or IFN gamma in the genital tract and protection against IVAG HSV 2 challenge following TLR ligand delivery . ^^^ Both TNF alpha ( / ) and IFN gamma ( / ) mice were protected against IVAG HSV 2 challenge following local delivery of poly ( 1 : C ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Vaccinia virus infection of XS 52 cells not only failed to elicit the production of various cytokines , including tumor necrosis factor alpha ( TNF alpha ) , interleukin 1beta ( IL 1beta ) , IL 6 , IL 10 , IL 12 p 40 , alpha interferon ( IFN alpha ) , and IFN gamma , it actively inhibited the production of proinflammatory cytokines TNF alpha and IL 6 by XS 52 cells in response to exogenous lipopolysaccharide ( LPS ) or poly ( 1 : C ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study , we investigated whether SJW modulated various pro inflammatory cytokines including interleukins ( IL 1beta , IL 2 , IL 6 ) , interferon ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) and intestinal epithelium apoptosis in rats . ^^^ Diarrhea , tissue damage , body weight loss , various cytokines including IL 1beta , IL 2 , IL 6 , IFN gamma and TNF alpha and intestinal epithelial apoptosis were monitored over 11 days . ^^^ The production of pro inflammatory cytokines such as IL 1beta , IFN gamma and TNF alpha was significantly up regulated in intestine . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro studies have demonstrated that proinflammatory cytokines , such as interferon gamma ( IFN gamma ) and tumor necrosis factor alpha ( TNF alpha ) , that are increased in the intestinal mucosa of patients with IBD can induce a leaky mucosal barrier . ^^^ This review summarizes barrier defects observed in IBD and addresses mechanisms by which proinflammatory cytokines , such as IFN gamma and TNF alpha , modulate AJC structure and epithelial barrier function . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma , IL beta , TNF alpha and nitric oxide in plasma and hydrocoele fluid . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1beta alone significantly induced IL 12 production in DCs , whereas TNF alpha or IFN gamma induced modest levels of IL 12 production . ^^^ In contrast , low dose LPS synergistically enhanced TNF alpha or IFN gamma induced IL 12 production . ^^^ SB 203580 , a specific inhibitor of p 38 MAPK , markedly inhibited TNF alpha or IFN gamma induced IL 12 production either in the absence or presence of LPS , but showed only modest effects on IL beta induced IL 12 production . ^^^ These findings suggest that the p 38 MAPK pathway is essential for the synergistic IL 12 production induced by TNF alpha or IFN gamma in combination with low dose LPS in DC . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Design of recombinant plasmids coding hybrids of immune interferon and human tumor necrosis factor ] . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor and immune interferon synergistically increase transcription of HLA class 1 heavy and light chain genes in vascular endothelium . ^^^ Tumor necrosis factor and immune interferon synergistically increase cell surface expression of class 1 major histocompatibility complex molecules in cultured human endothelial cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Recombinant tumor necrosis factor and immune interferon act singly and in combination to reorganize human vascular endothelial cell monolayers . ^^^ The addition of greater than or equal to 20 U / ml recombinant tumor necrosis factor ( rTNF ) or greater than or equal to 16 U / ml recombinant immune interferon ( rIFN gamma ) causes HECs 1 ) to become elongated , 2 ) to overlap , 3 ) to rearrange their actin filaments , and 4 ) to lose their stainable fibronectin matrix . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytocidal activity of human immune interferon ( IFN tau ) in combination with recombinant tumor necrosis factor alpha ( TNF ) was assessed in human colon carcinoma cell line HT 29 . ^^^ The synergistic cytocidal effect produced by immune interferon and tumor necrosis factor in HT 29 cells is associated with inhibition of rRNA processing and ( 2 ' , 5 ' ) oligo ( A ) activation of RNase L . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor and immune interferon synergistically induce cytochrome b 245 heavy chain gene expression and nicotinamide adenine dinucleotide phosphate hydrogenase oxidase in human leukemic myeloid cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lymphocytes can regulate hematopoietic cell growth by direct cellular contact or by releasing soluble factors , such as colony stimulating factors , immune interferon , lymphotoxin , and tumor necrosis factor , active on hematopoietic precursor cells . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immune interferon enhances functional properties of human granulocytes : role of Fc receptors and effect of lymphotoxin , tumor necrosis factor , and granulocyte macrophage colony stimulating factor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Effects of recombinant tumor necrosis factor , lymphotoxin , and immune interferon on proliferation and differentiation of enriched hematopoietic precursor cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We studied the effects of the immune mediators interleukin 1 , interleukin 2 , tumor necrosis factor , immune interferon , and lipopolysaccharide on the expression of the endothelial activation antigen recognized by the murine monoclonal antibody H4 / 18 in short term organ cultures of newborn foreskins . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Relationship with tumor necrosis factor and synergism with immune interferon . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The influence of 3 ' noncoding region ( ncr ) of the cDNA derived from an eukaryotic mRNA on the preceding structure gene expression in E . coli was investigated using human immune interferon ( GIF ) and human tumor necrosis factor ( TNF ) cDNAs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The potential of these expression vectors is demonstrated by comparing the expression levels of a number of mammalian cytokines ( human tumor necrosis factor , human immune interferon , human and murine interleukins 2 , murine interleukin 4 and murine fibroblast interferon ) , using these expression plasmids . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Anti CD 40 MoAb used alone or in combination with cytokines ( IL 1 alpha to IL 6 , interferon gamma , tumor necrosis factor gamma , and transforming growth factor beta ) failed to induce Ig secretion from B CLL cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines that have been thought to play a role in the pathophysiology of cachexia include tumor necrosis factor , interleukin 1 , interleukin 6 , interferon gamma and differentiation factor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After 30 days of culture with IL 2 the proliferative ability of TIL declined sharply in the majority of cases and TIL became refractory to any further stimulus , including addition of IL 4 , tumour necrosis factor alpha ( TNF alpha ) or interferon gamma , and activation with OKT 3 in solid phase . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We determine how recombinant interferon gamma ( IFN ) and tumor necrosis factor alpha ( TNF ) regulate plasminogen activation in monoblast like U 937 cells and normal human monocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A combination of lipopolysaccharide ( LPS ) , interferon gamma , tumor necrosis factor , and interleukin 1 stimulates the biosynthesis of large quantities of nitrite and nitrate ( NO 2 + NO 3 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A number of genes that are induced by type 1 interferons are also activated by one or more other inducers , including double stranded RNA , viruses , interferon gamma , interleukin 1 and tumor necrosis factor . ^^^ We show that double stranded RNA , virus , interferon gamma or tumor necrosis factor alpha can act directly to induce specific subsets of type 1 interferon inducible genes in the absence of any possible type 1 interferon involvement . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This protection is specific , and the TNF receptor does not stabilize interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokines , gamma interferon and TNF , also downregulate scavenger receptor expression in monocyte derived macrophages , leading to an inhibition of the transformation of these cells into foam cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this review the following cytokines will be reviewed : interleukin 1 and its inhibitor , tumor necrosis factor alpha and its inhibitor , interleukin 6 , interferon gamma , transforming growth factor beta , colony stimulating factors , fibroblast growth factors , platelet derived growth factor and prostaglandins . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pretreatment of supernatants with the antibodies to granulocyte / macrophage colony stimulating factor ( GM CSF ) or TNF alpha , but not interferon gamma , significantly diminished this enhancing effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The relative contributions of these hydrolytic enzymes to the cleavage of bioactive and radioiodinated cytokines including tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 alpha and interferon gamma was next examined . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This response of the plasma glutamine levels was significantly correlated with the production of interleukin 6 but not with that of interleukin 1 , tumor necrosis factor , or interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
As determined by immunocytochemistry and immunoprecipitation using a monoclonal anti ICAM 1 antibody , interleukin 1 alpha ( IL 1a ) , tumour necrosis factor alpha ( TNFa ) and interferon gamma ( IFNg ) strongly enhanced surface expression of ICAM 1 in both GO and normal OF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This activity could be blocked by neutralizing monoclonal antibodies against interferon gamma , but not by monoclonal antibodies against interleukin ( IL ) 2 , IL 4 , IL 5 , IL 6 , IL 10 and granulocyte macrophage colony stimulating factor ( GM CSF ) or even a polyclonal antibody against tumor necrosis factor ( TNF ) alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum concentrations of immunoglobulines , interleukin 1 , 3 and 6 , TNF alpha , interferon gamma and the soluble interleukin 2 receptor , leukocyte subsets and the natural killer cell activity were found to be within the normal range on both occasions , i . e . at normal and at high serum prolactin concentrations . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cells staining for interferon gamma , interleukin 2 ( IL 2 ) , and IL 2 receptors were more prominent than IL 4 , IL 5 , lymphotoxin ( LT ) , and tumor necrosis factor alpha ( TNF alpha ) , which occurred transiently in the second week and were associated with fewer cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The intrathecal immune response in neoplastic meningitis ( NM ) was studied by quantitation of immune parameters such as immunoglobulin G ( IgG ) ; IgM ; interleukins ( IL ) 1 , 2 , 4 , and 6 ; soluble IL 2 receptors ( sIL 2R ) ; interferon gamma ( IFNy ) ; tumor necrosis factor alpha ( TNF alpha ) ; and three tumor markers , carcinoembryonic antigen ( CEA ) , alpha fetoprotein ( AFP ) , and fibronectin ( FN ) , in 47 paired cerebrospinal fluid ( CSF ) and serum samples from patients with NM from different carcinomas , malignant melanoma , and lymphoma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Ocular inflammatory cells stained strongly positive for tumor necrosis factor alpha in all eyes with uveitis , but four of six uveitic eyes showed mild staining for tumor necrosis factor beta and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Intravenous administration of rIFN gamma followed by GLA 60 ( referred to as rIFN gamma / GLA 60 ) induced endogenous production of tumor necrosis factor ( TNF ) . ^^^ In contrast , neither rIFN gamma nor GLA 60 alone , nor GLA 60 / rIFN gamma ( reverse sequence of administration ) , which is unable to induce the production of TNF in the serum , had any effect . ^^^ Sera induced by the treatment with rIFN gamma / GLA 60 , and recombinant TNF , inhibited the in vitro growth of lung endothelial cells which is considered to be one of the essential events in tumor neovascularization . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
ICAM 1 protein expression could be enhanced within 4 h by cytokines , particularly after co incubation with interferon gamma ( IFN ) and tumour necrosis factor alpha ( TNF ) , interleukin 1 beta ( IL 1 ) , or IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Brains from OVA fed animals at the peak of disease showed perivascular infiltration with activated mononuclear cells which secreted the inflammatory cytokines interleukins ( IL ) 1 , 2 , 6 , 8 , TNF alpha , and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma and tumor necrosis factor synergize to induce nitric oxide production and inhibit mitochondrial respiration in vascular smooth muscle cells . ^^^ It was observed that the cytokines , interferon gamma and tumor necrosis factor alpha , synergistically induced an arginine dependent production of NO in these cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serial measurements were made during each treatment of interleukin 1 beta ( 2 1 beta ) , TNF , soluble IL 2 receptor ( sII 2r ) , soluble CD 4 ( sCD 4 ) , soluble CD 8 ( sCD 8 ) , interferon gamma ( IFNg ) and neopterin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Effect of interferon gamma on the production of tumor necrosis factor during acute Escherichia coli mastitis . ^^^ The effects of recombinant interferon gamma on the production of tumor necrosis factor in 10 dairy cows with Escherichia coli mastitis were determined . ^^^ Prophylactic administration of recombinant interferon gamma prior to the experimental E . coli challenge was effective in modifying the production of endogenous tumor necrosis factor during acute stages of disease . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Since the discovery that interferon inducers depress hepatic drug metabolism , the depressant action of cytochrome P 450 ( P 450 ) has been demonstrated to be shared by cytokines such as interferon alpha / beta and interferon gamma as well as interleukin 1 and tumor necrosis factor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These results , confirmed by both specific bioassay and by IL 6 ELISA , support the idea that acute GVHD in humans involves a cytokine cascade that includes production of IL 6 in addition to the previously reported involvement of tumor necrosis factor alpha and interferon gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human endothelial cells activated by interferon gamma , by interleukin 1 and TNF inhibit the replication of Toxoplasma gondii ] . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study examined combinations of the recombinant human cytokines , tumor necrosis factor alpha and interferon gamma , with doxorubicin and dactinomycin as well as other drugs on six squamous cell carcinoma cell lines of head and neck origin using the 3 ( 4 , 5 dimethylthiazol 2 yl ) 2 , 5 diphenyl tetrazolium bromide proliferation assay . ^^^ Interferon gamma significantly enhanced the cytotoxicity of tumor necrosis factor alpha with dactinomycin on all six cell lines investigated , while in four of six cell lines the cytotoxicity of tumor necrosis factor alpha with doxorubicin was significantly augmented by interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The PTX 3 mRNA is induced in endothelial , hepatic , and fibroblastic cells by IL 1 beta and tumor necrosis factor alpha but not by IL 6 and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Supernatants collected from cisplatin or rIFN gamma treated nMNC also demonstrated enhanced cytotoxicity against actinomycin D treated L 929 cells , suggesting that cisplatin or rIFN gamma treated nMNC release tumor necrosis factor ( TNF ) into the culture supernatant . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Role of tumor necrosis factor alpha and interferon gamma in newborn host defense against Listeria monocytogenes infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The combined effects of recombinant human tumor necrosis factor ( TNF ) , interferon gamma ( IFN ) and tamoxifen ( TAM ) on the proliferation of human breast cancer cell lines were investigated . ^^^ Synergistic cytotoxic effects of tumor necrosis factor , interferon gamma and tamoxifen on breast cancer cell lines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF stimulated resorption was inhibited by both interferon gamma and dexamethasone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This syndrome is due to the massive , although transient release in the circulation of various cytokines ( TNF , interferon gamma , interleukin 2 , interleukin 6 ) and represents one important side effect linked to in vivo use of OKT 3 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Simultaneous exposure of K 562 cells to rIFN gamma ( 600 units / ml ) and recombinant human tumor necrosis factor ( rTNF ; 1000 units / ml ) in combination with verapamil ( 50 microM ) resulted in a further increase of class 1 antigens in the cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Supernatants from OK 432 stimulated mononuclear cells , as determined by enzyme linked immunosorbent assays and radioimmunoassays , contained high levels of interleukin 8 ( IL 8 ; 1567 + / 145 pg / ml ) and tumor necrosis factor ( TNF alpha ; 2105 + / 152 pg / ml ) , low levels of leukotriene B 4 ( 800 + / 45 pg / ml ) and IL 1 beta ( 180 + / 22 pg / ml ) , but interferon gamma was not detectable . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma , more of a cachectin than tumor necrosis factor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Costimulation by recombinant human interferon gamma and IgE dependent triggering had a synergistic effect on TNF production , but it had only an additive action on IL 6 synthesis ( respective increase index : 9 . 8 compared with 2 . 9 and 9 . 8 compared with 2 . 1 , respectively , for BM from control and asthmatic subjects ) . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of endotoxin , dexamethasone and five recombinant DNA derived cytokines , interleukin 1 beta , tumor necrosis factor , and interferons alpha , beta and gamma , on constitutive and benzanthracene induced ethoxyresorufin O deethylase activity were examined in NCI H 322 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , recombinant interferon gamma augmented the amount of NO produced in response to TNF by both sensitive and resistant cells and correspondingly enhanced the susceptibility of resistant cells to TNF cytotoxicity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The concentrations of interleukin 1 , interferon gamma , tumor necrosis factor and prostaglandin E 2 were also found increased in the supernatant fluid of M phi cultured for 24 hours with 10 micrograms / ml of lipopolysaccharide . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Release of TNF was enhanced 20 fold by priming of the microglia with interferon gamma ; only pentoxifylline blocked the priming effect of interferon gamma on TNF release . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When purified RF was incubated with NK enriched cell lines from RA patients , increased transcription and subsequent production of interferon gamma and tumor necrosis factor alpha were observed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This pathway is operative in both normal and SCID mice and consists of at least four components : interferon gamma , tumor necrosis factor , macrophages , and natural killer cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Different molecular mechanisms lead to same endpoints with different function : TNF alpha induces non functional CSF 1 receptors on HL 60 cells in contrast to interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Factors such as IL 2 , interferon gamma , tumor necrosis factor alpha , and bovine insulin did not compete with IL 1 beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
BMM are subject to cell cycle arrest by numerous agents , including tumor necrosis factor alpha , interferon gamma , bacterial lipopolysaccharide , and agents that increase cAMP . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Culture of A 549 and Calu 1 cells with other cytokines and growth factors including GM CSF ( 500 units / ml ) , IL 3 ( 1 ng / ml ) , interferon gamma ( 500 units / ml ) , and tumor necrosis factor ( 50 units / ml ) had no significant effect on invasiveness . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
An interferon gamma , tumor necrosis factor , and interleukin 1 inducible , high output pathway synthesizing nitric oxide ( NO ) from L arginine was recently identified in rodents . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A pilot study of recombinant human TNF and interferon gamma in four patients with refractory AML . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Results from this study show that murine macrophages , activated by interferon gamma , and lipopolysaccharide or tumor necrosis factor alpha , both growth inhibit and kill M . tuberculosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Combined application of IL 2 and interferon alpha , interferon gamma , or tumor necrosis factor alpha in cultures for generation of activated killer cells significantly improved the effectiveness of cytotoxic mechanisms . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Highly purified human monocytes and monocyte derived macrophages were activated with interferon gamma ( IFN ) or tumour necrosis factor alpha ( TNF ) to antileukemic immune effector cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , we found that the synergistic effect of interferon gamma with LPS in induction of TNF release could also be counteracted by the addition of synthetic precursor Ia . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To characterize viral and immunological activity in the CNS , frozen specimens taken at autopsy from the cerebral cortex and white matter of HIV seropositive and seronegative individuals were stained immunocytochemically for mononuclear cells , major histocompatibility complex ( MHC ) antigens , HIV , astrocytes , and the cytokines interleukin 1 and 6 , tumor necrosis factor alpha and beta , and interferon gamma . ^^^ Levels of soluble CD 4 , CD 8 , and interleukin 2 receptor , as well as interferon gamma , tumor necrosis factor alpha , beta 2 microglobulin , neopterin , and interleukin 6 and 1 beta were assayed in the cerebrospinal fluid and plasma of many of these individuals during life . ^^^ Endothelial cells were frequently positive for interleukin 1 and interferon gamma and less frequently for tumor necrosis factor and interleukin 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor ( TNF ) , interleukin 1 ( IL 1 ) , and gamma interferon ( gamma IFN ) inhibit erythropoiesis in vivo and in vitro , and have been implicated in the pathogenesis of the anemia of chronic disease . ^^^ Tumor necrosis factor ( TNF ) , interleukin 1 ( IL 1 ) , and gamma interferon ( gamma IFN ) inhibit erythropoiesis in vivo and in vitro , and have been implicated in the pathogenesis of the anemia of chronic disease . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cells from the murine glioma 26 ( GL 26 ) , glioma 261 ( GL 261 ) , and ependymoblastoma A ( EpA ) cell lines were established in monolayer culture and treated for 48 hours with either alpha interferon , gamma interferon , tumor necrosis factor alpha , tumor necrosis factor alpha plus gamma interferon , or interleukin 2 . ^^^ The addition of tumor necrosis factor alpha to gamma interferon further increased Class 2 antigen expression on EpA tumor cells only . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Preincubation of either the effector or target cells with tumor necrosis factor or interferon gamma or addition of these cytokines directly to cytolytic assays did not alter the degree of cell lysis in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor ( TNF ) alpha and granulocyte macrophage colony stimulating factor ( GM CSF ) , but not interferon gamma , interleukin 4 or macrophage CSF , stimulated ingestion of the encapsulated fungal pathogen Cryptococcus neoformans by resident peritoneal macrophages in vitro . ^^^ Tumor necrosis factor ( TNF ) alpha and granulocyte macrophage colony stimulating factor ( GM CSF ) , but not interferon gamma , interleukin 4 or macrophage CSF , stimulated ingestion of the encapsulated fungal pathogen Cryptococcus neoformans by resident peritoneal macrophages in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although interferon gamma has been shown to effectively prime macrophages for enhanced production of tumor necrosis factor alpha ( TNF alpha ) , it is reasonable to assume that other cytokines present in the extracellular environment may likewise facilitate cytokine biosynthesis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
LPSw can prime macrophage to release TNF when given intradermally , percutaneously or even orally in mice as well as in humans , in exactly the same way as intravenous administration of interferon gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumour necrosis factor alpha ( TNF alpha ) was also investigated and shown to be a less powerful inducer than IL 2 or INF gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF production was induced by pretreatment with interferon gamma for 24 h and treatment with muramyl dipeptide . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Uptake of 50 microM glutamine was determined after a 12 hour incubation with varying doses ( 10 to 1000 U / mL ) of tumor necrosis factor alpha , interleukin 1 , interleukin 6 , interferon gamma , and various combinations of these cytokines . ^^^ Interferon consistently decreased glutamine transport by 30 % ; this was due to a reduction in carrier maximal transport velocity ( 3427 + / 783 pmol / mg protein / minute in controls versus 2279 + / 411 in interferon , p less than 0 . 05 ) rather than a change in Km ( 276 + / 29 microM in controls versus 333 + / 74 in interferon , p = not interferon + dexamethasone + tumor necrosis factor + interleukin 1 resulted in a 38 % decrease in transport activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor enhanced the accumulation of c sis mRNA and interferon gamma decreased its level . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Additional treatment with interferon gamma enhanced production of TNF in both subsets , but CD14+ / CD16+ small monocytes still exhibited lower levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inhibition of rat bladder tumor ( RBT 323 ) growth by tumor necrosis factor alpha and interferon gamma in vivo . ^^^ The antiproliferative activities of recombinant rat gamma interferon and recombinant human tumor necrosis factor alpha were evaluated in a new rat bladder tumor model , RBT 323 . ^^^ Gamma interferon and tumor necrosis factor monotherapy starting two days after tumor implantation showed dose dependent growth inhibiting effects in the dose range tested . ^^^ Combination treatment with gamma interferon and the highest dose of tumor necrosis factor resulted in synergistic antiproliferative effects . ^^^ Gamma interferon treatment initiated at a tumor volume of 0 . 2 to 0 . 5 cm . 3 had no antitumor effects , whereas treatment with 100 micrograms . tumor necrosis factor per rat inhibited tumor growth significantly . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To examine the response of the hypothalamic pituitary adrenal ( HPA ) axis to severe surgical stress , we measured the immunoreactive plasma levels of corticotropin releasing hormone ( CRH ) , corticotropin , cortisol , arginine vasopressin ( AVP ) , atrial natriuretic factor ( ANF ) , neuropeptide Y ( NPY ) , interleukin 1 ( IL 1 ) , IL 6 , interferon gamma ( INF ) , and tumor necrosis factor alpha ( TNF alpha ) in eight patients with Zollinger Ellison syndrome ( ZES ) or mediastinal parathyroid carcinoma , all undergoing major surgery with a standardized anesthetic technique . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Incubation of IELs with interferon gamma and tumour necrosis factor ( TNF ) did not induce cytotoxic activity , but TNF could augment the levels of IL 2 induced cytotoxicity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We reached these conclusions on the basis of the following results ; ( 1 ) agents ( sodium nitroprusside and isobutylmethylxantine ) that cause the accumulation of cGMP intracellularly increased the TNF sensitivity of TNF resistant cells ; ( 2 ) the addition of dibutyryl cGMP to TNF resistant cells increased the TNF sensitivity ; and ( 3 ) treatment at 40 degrees C or agents such as interferon gamma and actinomycin D , that synergistically kill tumor cells together with TNF , potentially increased the cGMP level . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Activation and fixation of C 3 by human B cell lines is enhanced by interferon gamma and tumor necrosis factor alpha . ^^^ All cell lines activated C 3 with higher efficiency when treated with interferon gamma or tumor necrosis factor alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the in vitro effects of both psychoactive and nonpsychoactive marijuana components on leukocyte secretion of the immunoregulatory cytokines interleukin 1 alpha ( IL 1 ) , tumor necrosis factor alpha ( TNF ) , interferon gamma ( IFN ) and interleukin 2 ( IL 2 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma also stimulated 1 , 25 ( OH ) 2D production at low concentrations , but this was not additive to that by TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokines comprised interferon gamma and tumor necrosis factor alpha . ^^^ Interferon gamma was present during the acute , remitting , and relapsing phases and was localized to inflammatory cells , whereas tumor necrosis factor occurred at low levels only . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In a proportion of originally negative or low expressor tumors , in vitro treatment with Interferon gamma and tumor necrosis factor alpha induced the expression of class 1 antigens and / or ICAM 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We analyzed the modulating capacity of the lymphokines recombinant interferon gamma ( rIFN gamma ) , interleukin 4 ( IL 4 ) , and recombinant tumor necrosis factor alpha ( rTNF alpha ) on class 2 expression in subsets of alloactivated human T lymphocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of ICAM 1 on the surface of human umbilical vein endothelial cells and a human lung carcinoma cell line ( A 549 ) was increased by interleukin 1 beta , tumor necrosis factor alpha , and interferon gamma in a concentration dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neutrophils do not adhere to unstimulated neural cells but will bind to neural cells that have been exposed to tumor necrosis factor alpha ( TNF alpha ) and in some cases other cytokines such as gamma interferon ( gamma IFN ) or interleukin 1 ( IL 1 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Those signals generated following infection include interleukins 1 , 2 , and 6 , interferon gamma , and tumor necrosis factor ( or cachectin ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Endothelial cell production of nitrogen oxides in response to interferon gamma in combination with tumor necrosis factor , interleukin 1 , or endotoxin . ^^^ In this study , we tested interferon gamma , tumor necrosis factor , interleukin 1 , interleukin 2 , muramyl dipeptide , and endotoxin for their effects on production of nitrogen oxides by endothelial cells . ^^^ Interferon gamma , in combination with tumor necrosis factor , interleukin 1 ( IL 1 ) , or endotoxin , induced murine brain endothelial cells to secrete nitrites ( 20 45 microM within 48 hr ) , which are breakdown products of nitric oxide . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
None of the other recombinant lymphokines tested ( IL 1 , IL 2 , IL 3 , IL 5 , IL 6 , interferon gamma , tumor necrosis factor alpha and beta , granulocyte macrophage colony stimulating factor ) decreased CD 14 expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Other cytokines that regulate acute phase gene expression and protein synthesis include IL 1 , tumor necrosis factor alpha , interferon gamma , as well as other stimulatory factors and cofactors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunohistochemistry on culture CE cells using monoclonal anti ICAM 1 antibody yield positive staining that increased after exposure to interleukin 1 beta ( IL 1 beta ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( gamma IFN ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Of IL 1 , IL 2 , IL 4 , IL 6 , tumor necrosis factor , and interferon gamma only IL 2 specifically enhanced CD 8 survival in the HIV group , compared to the control group . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Gel mobility shift assays demonstrate that TNF , IL 1 , or LPS ( but not IL 2 , IL 4 , IL 6 , interferon gamma , histamine , or transforming growth factor beta ) induces activation of NF kappa B like DNA binding activity in HUVEC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have previously reported that cultured murine brain endothelial cells ( MBE ) produce large quantities of nitric oxide ( NO ) after activation with interferon gamma in combination with any of several immunoactivators including : bacterial endotoxin , tumor necrosis factor and interleukin 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MAb against intercellular adhesion molecule type 1 ( ICAM 1 ) and LFA 1 also prevented binding of T lymphocytes , but had a more marked effect on adhesion to SSc fibroblasts than to normal fibroblasts ; they also completely abolished the increased binding to fibroblasts treated with interleukin 1 alpha , tumor necrosis factor alpha , and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The presented data show that treatment of lung lymphocytes with human recombinant interleukin 2 ( rIL 2 ) , purified rat interferon alpha / beta ( IFN alpha / beta ) , or murine recombinant tumor necrosis factor alpha ( rTNF alpha ) resulted in a dose dependent increase in LLNK activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We now show that this resistance correlates with the ability of macrophages to synthesize nitric oxide ( NO ) following activation with interferon gamma or tumor necrosis factor alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Whole cell enzyme linked immunosorbent assays , a tritiated thymidine uptake assay , and a colorimetric assay of mitochondrial function were used to assess how four cytokines ( interleukin 1 , interleukin 2 , interferon gamma , and tumor necrosis factor alpha ) affect endothelial cell metabolism and susceptibility to BTV infection . ^^^ Interferon gamma and tumor necrosis factor alpha suppressed viral antigen expression and were synergistic early in the infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Until recently the presence of cytokines in colostrum was unsuspected but it has been now clarified that normally there are at least four cytokines , namely interleukin 1 and 6 , tumor necrosis factor and interferon gamma , that may exert an important immunostimulatory role particularly on the oropharyngeal associated lymphoid tissue . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Like IL 2 , IL 7 stimulated production of tumor necrosis factor alpha , but we failed to detect interferon gamma in IL 7 stimulated cultures . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
High dose recombinant tumor necrosis factor alpha in combination with interferon gamma and melphalan in isolation perfusion of the limbs for melanoma and sarcoma . ^^^ PURPOSE : To determine the toxicity and the therapeutic efficacy of the combination of the recombinant tumor necrosis factor alpha ( rTNF alpha ) , recombinant interferon gamma ( rIFN gamma ) , and melphalan , we designed a protocol using isolation limb perfusion ( ILP ) with hyperthermia for in transit metastases of melanoma and recurrent sarcoma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These toxic effects occur by direct action of TNF on host cells and by the interaction with a cascade of other endogenous mediators including interleukin 1 and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma also blocked this TNF action when added simultaneously . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment with interferon gamma restored ( p less than or equal to 0 . 05 ) hemorrhage induced suppression of pM phi antigen presentation capacity and Ia antigen expression and increased ( p less than or equal to 0 . 05 ) interleukin 1 and tumor necrosis factor release by pM phi and sM phi , as well as splenocyte proliferation ( p less than or equal to 0 . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The dermatological changes were associated with elevated interferon gamma and tumor necrosis factor alpha serum levels ( immunoradiometric assay ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The concentrations of interleukin 1 , interferon gamma , tumor necrosis factor and prostaglandin E 2 in the media of 24 hour cultured macrophage with 10 micrograms / ml of LPS were also increased throughout the treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha release was enhanced by pretreatment of PAM with rhIFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Like CsA , Ro 14 6113 inhibited the mitogen or alloantigen induced proliferation of T cells as well as their capacity to secrete interleukin 2 ( IL 2 ) , interferon gamma and tumor necrosis factor alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , it is likely that several cytokines can cause changes in neuroendocrine function , and we have now investigated a series of others for central activity on the HPA : IL 2 , IL 6 , IL 8 , tumor necrosis factor ( cachectin ) , interferon alpha 2 , and interferon gamma . ^^^ Neither IL 2 ( 1 10000 U / ml ) , IL 8 ( 0 . 1 10 nM ) , tumor necrosis factor ( 10 1000 U / ml ) , interferon alpha 2 ( 10 1000 U / ml ) nor interferon gamma ( 10 1000 U / ml ) had any effect on hypothalamic CRH 41 release or pituitary ACTH release . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We examined effects of priming with recombinant human interferon gamma ( IFN ) or tumor necrosis factor alpha ( TNF ) on neutrophil responses to Candida albicans hyphae . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Notably , a number of cytokines tested ( interleukin 1 to 8 , interferon gamma , TNF alpha and beta ) did not influence the expression of either the 75 kDa or the 55 kDa TNFR when given to resting B cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Possible sources of systemic TNF alpha activity are , in particular , tissue macrophages of the recipient type that are stimulated by pretransplant conditioning and endotoxin and subsequently further activated by interferon gamma released by donor lymphocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Of the cytokines assayed , only recombinant murine tumor necrosis factor alpha and recombinant human tumor necrosis factor beta demonstrated measurable cytotoxic activity when assayed independently , while recombinant human interferon gamma was the only cytokine to demonstrate greater than additive activity in combination with rHuTNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Third , monocytes incubated with liposome CGP 31 , 362 released both tumor necrosis factor ( TNF ) and interleukin 1 activities , whereas monocytes treated with liposome CGP 19 , 835 ( in the absence of interferon gamma ) released only TNF activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma treatment had no significant effect on basal ODC activity but inhibited TNF mediated ODC induction by approximately 50 % . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( 200 U / ml ) further induced production of tumor necrosis factor from mononuclear cells of 11 women with salpingitis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The present study was performed in order to evaluate the effect of suramin , recombinant human tumor necrosis factor ( TNF ) , interferon gamma and the combination of suramin plus TNF or interferon gamma on proliferation of PC 3 , a human , hormone unresponsive prostate carcinoma cell line . ^^^ The effect of suramin , tumor necrosis factor and interferon gamma on human prostate carcinoma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The data presented extend to a larger series of 27 consecutive renal allograft recipients treated prophylactically with OKT 3 our previous observation that the acute OKT 3 induced clinical syndrome is related to massive release in the circulation of some cytokines , among which are tumor necrosis factor and interferon gamma . ^^^ Results confirm that when corticosteroids are given in sufficient amount and , importantly , 1 hr before the first OKT 3 injection , they significantly decrease the release of both tumor necrosis factor and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of soluble mediators also were tested ; recombinant human tumor necrosis factor alpha ( TNF ) but not recombinant rat interferon gamma or endotoxin was directly lethal to P . carinii . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These parasite killing effects in crisis serum are due to the presence in the serum of cytokines tumor necrosis factor and interferon gamma , which are produced by the host as a result of the malaria infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When administered immediately before challenge , anti tumor necrosis factor ( TNF ) antibody abrogated the ear swelling response in CH ; antibody directed against interferon gamma or antibodies to both granulocyte / macrophage colony stimulating factor and interleukin 3 ( IL 3 ) had a partial inhibitory effect ; anti IL 2 receptor antibody had no effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition to rMuIFN gamma , recombinant human interleukin 1 beta and recombinant human tumor necrosis factor alpha were also effective on rescue from the lethal infection with Listeria monocytogenes in neonatal mice , but the effect was seen only in the limited doses . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Leishmanial infection of human monocyte derived macrophages enhanced the basal TNF alpha production by these cells , and this effect was further potentiated by treatment with recombinant interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover TNF and acid labile interferon alpha might contribute to HIV replication and disease progression . ^^^ Tumor necrosis factor and interferon as prognostic markers in human immunodeficiency virus ( HIV ) infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cloned lines ascertained to be of endothelial origin were incubated in vitro with interferon gamma and tumor necrosis factor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pre incubation of MO for 24 h with interferon gamma or tumor necrosis factor ( TNF ) increased their ability to kill yeast targets . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon alpha induces expression of the CD 69 activation antigen in human resting NK cells , while interferon gamma and tumor necrosis factor alpha are ineffective . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It is modulated by interferon gamma , prostaglandin E 2 and indomethacin in the same manner as observed with tumour necrosis factor ( TNF ) release from human monocyte / macrophage cells cultured in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neither control untreated HPBM nor recombinant interferon gamma ( rIFN gamma ; 1 U / ml ) treated HPBM alone released soluble TNF activity . ^^^ Treatment of HPBM with LPS induced TNF transcription as late as 5 days of in vitro culture with maximal induction occurring during the first 48 h . rIFN gamma significantly induced TNF transcription at 24 h in the absence of soluble TNF activity , but did not increase transcription at later times . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Alterations caused by Candida albicans , lipopolysaccharide ( LPS ) and recombinant interferon gamma ( rIFN gamma ) were similar in SAVO , C57BL / 6 , C3H / HeN and C3H / HeJ mice , and were not correlated with an activation process as shown by the amount of tumor necrosis factor alpha ( TNF alpha ) being released . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The amplification process involved the use of 5 ' and 3 ' primers for gamma interferon , and tumor necrosis factor alpha , with beta actin serving as a control . ^^^ Gamma interferon mRNA , but not tumor necrosis factor alpha mRNA , was detectable in 4 of 5 psoriatic epidermal specimens . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation of multiple CD8+ murine tumor infiltrating lymphocyte ( TIL ) lines and one TIL clone with the tumor of origin of the TIL induced at least three fold more secretion of TNF and / or INF gamma than was elicited by other syngeneic , methylcholanthrene ( MCA ) induced sarcomas . ^^^ Identification of unique murine tumor associated antigens by tumor infiltrating lymphocytes using tumor specific secretion of interferon gamma and tumor necrosis factor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In two of two patients tested , there was a transient though major rise in the levels of interferon gamma and tumor necrosis factor in the first 12 hours of treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Secretion of IL 1 and TNF was observed in PM stimulated with LPS or MDP but not with rMuIFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study we evaluated the antiproliferative effects of human recombinant alpha interferon ( IFN ) , gamma interferon and tumor necrosis factor alpha ( TNF ) on eight human RCC xenografts . ^^^ After 6 weeks of therapy consisting of 150 or 1 , 500 units IFN / g given s . c . peritumorally three times a week or 30 , 000 units TNF / g given five times a week , alpha IFN treatment resulted in 2 % 100 % growth inhibition ; gamma IFN , in 7 % 80 % ; and TNF , in 35 % 75 % as compared with the untreated control . ^^^ Differential sensitivity of renal cell carcinoma xenografts towards therapy with interferon alpha , interferon gamma , tumor necrosis factor and their combinations . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN ) has been found to reversibly inhibit chlamydial growth at an early stage in the replicative cycle , while tumor necrosis factor ( TNF ) has a more profound effect on chlamydial growth resulting in production of aberrant reticulate bodies and enhancement of production of prostaglandin E 2 ( PGE 2 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The macrophage mediated cytotoxicity was significantly enhanced when TNF primed macrophages were treated with cisplatin , LPS , FK 565 or interferon gamma for 24 h , compared to unprimed and treated macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
High release of tumor necrosis factor alpha , interferon gamma and interleukin 6 by adherent lymphokine activated killer cells phenotypically derived from T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Heat inactivation of plasma abolished TNF production , whereas prior treatment with interferon gamma augmented the response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Under these conditions many cellular functions are regulated by mediators e . g . transforming growth factor beta , tumor necrosis factor alpha , interferon gamma or interleukin 1 alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Replicate cultures were also exposed to recombinant human tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 ( IL 1 ) or interferon gamma for 1 to 3 h before the measurement of hyaluronan uptake . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In HR of the A CSID group , the production of TNF alpha and IL 1 beta was augmented and interferon gamma production was also increased in these patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , even in the absence of interferon gamma , human monocytes released significant levels of tumor necrosis factor and interleukin 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 2 has additional effects on other components of the cellular immune system , including B cells and macrophages , and induces secretion of other soluble mediators , including tumor necrosis factors ( TNF ) alpha and beta , and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Probably due to their pleiotropic nature , systemic administration of cytokines such as interleukin 1 , interleukin 2 , tumor necrosis factor , and interferon gamma may exert either accelerating or suppressive effects on autoimmune diseases . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In cultured PT cells , ICAM 1 is expressed at basal levels in PT and is increased by the cytokines interferon gamma , IL 1 alpha , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro treatment of the tumor cell suspensions with interferon gamma and tumor necrosis factor alpha ( TNF alpha ) induced or enhanced the ICAM 1 and / or class 1 antigen expression in 8 / 12 cases . ^^^ The results show thus ( a ) that the lymphocytes react in vitro with tumor cells only if these express both MHC class 1 molecules and ICAM 1 ; ( b ) that expression of these molecules can be induced by interferon alpha and TNF alpha ; ( c ) that cytotoxic effectors generated in the MLTC with cytokine treated tumors can also act on the untreated tumor cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma and tumor necrosis factor alpha also prevent the onset of lymphomas . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , interferon gamma ( INF gamma ) upregulated the amount of TNF induced by rGM CSF in all donors examined , with variable effect on IL 1 alpha and IL 1 beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
While treatment with recombinant interferon gamma increased the number of TNF receptors by 53 + / 8 % , E , 1 , G , S , ACTH and An decreased the number of receptors by 81 + / 6 % , 83 + / 6 % , 15 + / 5 % , 83 + / 4 % , 17 + / 4 % and 21 + / 4 % , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human melanocyte expression of intercellular adhesion molecule 1 ( ICAM 1 ) with or without stimulation by interferon gamma ( IFN G ) , tumor necrosis factor alpha ( TNF alpha ) , or interleukin 1 alpha ( IL 1 alpha ) , was measured utilizing direct immunofluorescence and fluorescence activated cell sorting ( FACS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Similar findings , relating to prior chemotherapy treatment status , were obtained for tumor necrosis factor and interferon gamma , but not for interleukin 2 or interferon alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Monocytes exposed to inactivated streptococci , phorbol 12 myristate 13 acetate , and tumor necrosis factor showed augmented levels of hsp 70 transcripts , whereas interferon gamma and monocyte , granulocyte , and granulocyte monocyte colony stimulating factors had no effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There is growing evidence that cytokines ( interleukin [ IL ] 1 , IL 6 , interferon gamma , tumor necrosis factor [ TNF ] ) directly or indirectly interfere with the intrahepatic development of malaria parasites . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Recently , the compounds have also been found to be inducers of interferon gamma and tumor necrosis factor in human peripheral blood leukocytes ( PBL ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The function of accessory cells the dendritic cells , macrophages , etc . is stimulated by PRFs such as interferon gamma , IL 1 , TNF , GM CSF and IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This finding suggests that chronic immune activation , possibly along with the release of specific cytokines such as interferon gamma and tumor necrosis factor alpha may be involved in the pathogenesis of anaemia . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF production was also elevated in these patients when PBMC were stimulated with interferon gamma ( p less than 0 . 05 ) or LPS ( p = 0 . 035 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
For the release of IL 1 beta , IL 6 , and TNF alpha a synergistic effect of interferon gamma ( IFN g ) and lipopolysaccharide ( LPS ) was observed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A disturbance in the production of cytokines may be involved , since exogenous cytokines , such as Interferon gamma or Tumor Necrosis Factor a , can inhibit radiation induced lymphomagenesis , reproducing the effects of bone marrow transplantation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Control experiments demonstrated that MCA is identical to neither the T cell derived lymphokines IL 2 to IL 6 , IL 9 , interferon gamma , tumor necrosis factor alpha or beta , or granulocyte macrophage colony stimulating factor ( CSF ) , nor to IL 7 , granulocyte CSF , macrophage CSF , erythropoietin , leukemia inhibitory factor , or epidermal growth factor ( EGF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In patients with sepsis , the total monocyte TNF alpha levels were increased despite a concomitant increase in their prostaglandin E 2 levels in both stimulated ( interferon gamma plus muramyl dipeptide ) and unstimulated in vitro assays ( 9 patients : stimulated prostaglandin E 2 range , 30 . 1 to 123 . 6 ng / 10 ( 6 ) monocytes per milliliter ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Maintenance and cure of the L5178Y murine tumor dormant state by interleukin 2 : dependence of interleukin 2 on induced interferon gamma and on tumor necrosis factor for its antitumor effects . ^^^ We previously reported that MuIFN gamma requires the action of murine tumor necrosis factor ( MuTNF ) to induce cytotoxic activity in PC cultures from tumor dormant mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study evaluated the effect of cefoxitin , interferon gamma ( INF gamma ) , and tumor necrosis factor alpha ( TNF alpha ) on the development of a polymicrobial soft tissue infection . ^^^ Animals received either no treatment , cefoxitin , recombinant rat INF gamma , recombinant human TNF alpha , or cefoxitin / cytokine combinations . ^^^ Although neither INF gamma nor TNF alpha alone had a salutary effect when given with cefoxitin in animals after shock , INF gamma and TNF alpha reduced abscess size by 50 % and 55 % , respectively . ^^^ These results suggest that INF gamma and TNF alpha may be useful to reduce the severity of mixed gram negative infections after shock with bacterial contamination . . ^^^ Interferon gamma and tumor necrosis factor alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
As determined by the use of neutralizing antisera , the soluble factor ( s ) are not tumor necrosis factor and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Further , these TILs demonstrated activity in vitro against the non class 1 expressing melanoma treated with the combination of murine recombinant interferon gamma and human recombinant tumor necrosis factor alpha ; in vivo , when administered with recombinant interferon gamma and recombinant tumor necrosis factor alpha , TILs from the class 1 expressing tumor mediated regression of non class 1 expressing pulmonary metastases , presumably by augmenting class 1 expression . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effect of both tumor necrosis factor alpha ( TNF ) and interleukin 1 ( IL 1 ) on interferon gamma ( IFN ) induced Ia expression was studied using cultured endothelial cells ( EC ) isolated from cerebral microvessels of SJL mice . ^^^ The inhibitory effect of tumor necrosis factor and interleukin 1 on Ia induction by interferon gamma on endothelial cells from murine central nervous system microvessels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFN ) and tumor necrosis factor alpha ( TNF ) were examined for their ability to enhance major histocompatibility complex ( MHC ) expression on a variety of human tumor and normal tissue targets . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human myelogenous leukemic cell lines ( ML 1 , U 937 , THP 1 ) were induced to differentiate by treatment with various cytokines such as tumor necrosis factor , interferon gamma and interleukin 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The RNA message for interferon gamma was detected by in situ hybridization in TIL induced with interleukin 2 and tumor necrosis factor alpha but not in freshly isolated TIL . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The response of the neonate ' s effector cells to the immunoenhancing effects of interferon gamma appears to be variable ; diminished enhancement by interferon gamma of cytotoxic cell function and the production of tumor necrosis factor by macrophages may further compound the effects of diminished production of interferon gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It was found that BV enhances the natural killer ( NK ) activity of PBMC and increases their spontaneous and phytohemagglutin ( PHA ) induced production of tumor necrosis factor alpha and interleukin 2 as well as the PHA stimulated production of interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This system has been used to evaluate the immunotherapeutic activity of macrophages , specifically reactive T cells ( CTL / RFB ) , lymphokine activated killer cells and interleukin 2 , and tumor necrosis factor alpha and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
KC activated by rhM CSF produced less TNF and concomitantly demonstrated a lower cytotoxicity against MCA 26 cells when compared with KC activated by gamma interferon ( gamma IFN ) with or without lipopolysaccharide ( LPS ) . ^^^ M CSF did not act in a synergistic fashion with gamma IFN and LPS to increase TNF secretion or cytotoxicity against MCA 26 cells . rhM CSF thus acts as a single agent capable of activating murine KC to a cytotoxic state but does not cooperate with classical priming / triggering signals to achieve KC activation . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found that no single cytokine , but rather a specific combination of tumor necrosis factor , interleukin 1 , interferon gamma , and endotoxin , were required for maximal induction of HC nitrogen oxide production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of TNF alpha on SP A were distinct from the effects of interferon gamma which increased SP A content approximately twofold in H 441 4 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A phase 2 trial of recombinant human interferon gamma and recombinant tumor necrosis factor in patients with advanced gastrointestinal malignancies : results of a trial terminated by excessive toxicity . ^^^ Recombinant human tumor necrosis factor and recombinant human interferon gamma are two representatives of a novel class of antineoplastic agents . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Activation of murine macrophages by tumor necrosis factor , interleukin 1 , interferon gamma and cisplatin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have tested sera from patients presenting with a febrile illness admitted to hospital in Honiara , Solomon Islands , for the presence of TNF , interferon gamma , and interleukin 1 ( IL 1 ) . ^^^ Serum TNF concentrations correlated with malarial parasite density and the patients ' temperatures , but not with interferon or IL 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
No significant activity of TNF was seen against trophozoite induced P . cynomolgi B infection . rHuIFN ( gamma ) inhibited schizogony in functional human hepatocytes infected with P . falciparum sporozoites . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
No synergistic effect was seen if TNF alpha was given in combination with interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Some CD 3 clones produced high levels of interferon gamma , tumour necrosis factor alpha ( TNF alpha ) and transforming growth factor beta ( TGF beta ) upon stimulation , but there was no relationship between specific cytokine production and cell clone phenotype or cytotoxic function . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A further three markers showed increased expression in 7 day old macrophages stimulated by IFN , namely a high affinity Fc gamma receptor ( 10 . 1 ) , MHC Class 2 ( 1B5 ) and tumour necrosis factor ( TNF ) . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Supernatant of LPS activated monocytes ( HSF ) and recombinant interleukin 4 ( rIL 4 ) , but not recombinant IL 1 beta , IL 2 , IL 6 , TNF alpha , GM CSF , and interferon gamma increased [ 3H ] thymidine incorporation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation of MO7E cells with 12 0 tetradecanoyl phorbol 13 acetate ( TPA ) or cytokines such as G CSF , M CSF , interleukin 1 ( IL 1 ) , interleukin 4 ( IL 4 ) , interleukin 6 ( IL 6 ) , interferon gamma , tumor necrosis factor ( TNF ) , or transforming growth factor beta ( TGF beta ) did not induce tyrosine phosphorylation of pp 93 or pp 70 , suggesting that these two phosphoproteins are specific for GM CSF or IL 3 induced activation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Thioglycolate elicited peritoneal macrophages were readily activated to significantly increased levels of intracellular TNF , as early as 1 hr after activation with lypopolysaccharide ( LPS ) + interferon gamma : maximum intracellular TNF was evident after 2 3 hr . ^^^ Both LPS and interferon gamma was necessary to increase intracellular TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After stimulation with endotoxin or endotoxin plus interferon gamma , HIV 1 infected monocytes / macrophages produced amounts of TNF alpha , IL 6 , GM CSF , and IL 1 beta comparable to that of uninfected cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Several inducers have been employed : dimethylsulfoxide ( DMSO ) granulocyte macrophage colony stimulating factor ( GM CSF ) ; tunicamycin , interferon gamma , tumor necrosis factor and lipopolysaccharide . 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Other means of enhancing the activity of interleukin 2 based immunotherapy are suggested by the finding that tumor cell susceptibility to lysis by natural killer cells is depressed following treatment with interferon gamma and tumor necrosis factor , but susceptibility to lysis by tumor infiltrating lymphocytes is markedly enhanced . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We now demonstrate that the presence of the macrophage specific colony stimulating factor 1 ( CSF 1 ) inhibits the priming of murine macrophages by a variety of agents including tumor necrosis factor alpha , granulocyte / macrophage colony stimulating factor , interferon gamma , and bacterial lipopolysaccharide . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Peripheral blood monocytes isolated by centrifugal elutriation from buffy coats of healthy donors were incubated with lipopolysaccharide ( LPS ) , lipopeptide derived from Escherichia coli ( CGP 31362 ) , recombinant human interferon gamma ( rIFN gamma ) plus muramyl dipeptide ( MDP ) or recombinant human TNF for 24 hr . ^^^ The human melanoma A 375 M ( highly sensitive to TNF ) , A 375 P ( moderately sensitive to TNF ) , and A 375 R ( resistant to TNF ) cells were all lysed in vitro by monocytes activated with LPS , CGP 31362 , or MDP plus rIFN gamma . ^^^ The cytolytic activities of monocytes incubated with LPS , CGP 31362 , and MDP plus rIFN gamma were not affected by the presence of anti TNF antiserum , whereas the lysis of A 375 M cells by monocytes incubated with rTNF was completely abolished by the presence of anti TNF antiserum . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
While the exact mechanisms of IL 2 based immunotherapy in cancer remain unclear , it has been hypothesized that both the IL 2 activated lymphocyte and its secretory products such as interferon gamma or tumor necrosis factor beta may contribute to the lysis of tumor cells in vivo . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Uptake of [ 3H ] acetate by adipocytes and heparin releasable lipoprotein lipase activity was inhibited after treatments of the cells with picomolar concentrations of recombinant human tumor necrosis factor alpha ( rHuTNF alpha ) , human tumor necrosis factor beta ( rHuTNF beta , also called lymphotoxin ) , murine interferon gamma ( rMuIFN gamma ) , and a human hybrid interferon alpha [ rHuIFN alpha 2 / alpha 1 ( Bgl 2 ) ] . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Several human biologic response modifiers , including HuIFN gamma , HuIFN beta , HuIFN alpha , interleukin 2 ( HuIL 2 ) , and tumor necrosis factor alpha , have now been assessed for their ability to enhance tryptophan degradation in human peripheral blood mononuclear cell ( PMC ) cultures . ^^^ Significantly increased amounts of tryptophan catabolites were observed after treatment with HuIFN gamma , HuIFN beta , HuIFN alpha , and HuIL 2 , but not human tumor necrosis factor alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Addition of recombinant human tumor necrosis factor , affinity purified interferon alpha , or interferon gamma to PMN caused increased antifungal activity in PMN . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The odyssey of an interferonologist : from interferon gamma to Down syndrome , tumor necrosis factor , and interleukin 1 , and back again . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These include interferon alpha and beta , interferon gamma , interleukin 2 , interleukin 1 , tumor necrosis factor and lymphotoxin , B lymphocyte growth factors , hemopoietic growth factors , and others . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Depression of liver drug metabolism and increase in plasma fibrinogen by interleukin 1 and tumor necrosis factor : a comparison with lymphotoxin and interferon . ^^^ Different recombinant cytokines were studied for their effects on mouse liver in vivo : interleukin 1 alpha and beta , tumor necrosis factor , lymphotoxin , interferon alpha A / D and gamma depressed liver cytochrome P 450 dependent drug metabolism ( measured by ethoxycoumarin deethylase activity ) 24 h after treatment , at doses in the microgram range , while IL 2 had no effect on this enzymatic system . ^^^ Interferon gamma and tumor necrosis factor had an additive effect in depressing liver drug metabolism . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inhibitory effect of human recombinant interferon gamma on synthesis of acute phase proteins in human hepatoma Hep G 2 cells stimulated by leukocyte cytokines , TNF alpha and IFN beta 2 / BSF 2 / IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this report , we show that CD 28 stimulation augments T cell immune responses by specifically inducing a 5 to 50 fold enhancement in the expression and secretion of interleukin 2 , tumor necrosis factor type alpha , lymphotoxin , interferon gamma , and granulocyte macrophage colony stimulating factor in normal human T cells stimulated to proliferate by crosslinking of the T cell receptor / CD3 complex . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Fibroblasts were weakly stimulated by recombinant ( rec ) tumor necrosis factor ( rec TNF alpha ) ; they did , however , not proliferate in response to mitogens , lipopolysaccharide , rec granulocyte macrophage colony stimulating factor ( rec GM CSF ) , macrophage CSF , rec interferon gamma , insulin or transferrin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The addition of lymphokines such as interleukin 1 ( IL 1 ) , interleukin 3 ( IL 3 ) , tumor necrosis factor ( TNF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and interferon gamma ( IFN y ) did not improve the reactions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition to their ability to lyse certain tumor target cells , they release lymphokines including tumor necrosis factor and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The activation of vascular endothelial cells may be also of special importance for the cellular adhesion of lymphocytes and monocytes and their interactions by interleukin 1 , tumor necrosis factor , lymphotoxin and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A combination of recombinant human tumor necrosis factor ( rhTNF ) , recombinant mouse TNF ( rmTNF ) , recombinant human interleukin 1 ( rhIL 1 ) or bacterial lipopolysaccharide with recombinant mouse interferon gamma ( rmIFN gamma ) resulted in the synergistic induction of cytostatic activity in RPM . ^^^ These results suggest that the cytostatic activity of RPM was augmented by the LCM , and that the effect of the LCM may be not due to IFN gamma , TNF , GM CSF , IL 1 or a small amount of contaminating lipopolysaccharide . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lymphocytes bind to cultured keratinocytes that are treated with interferon gamma ( IFN gamma ) and tumor necrosis factor ( TNF ) . ^^^ The modulation of these cell surface antigens by IFN gamma and TNF with the use of short term organ cultures of skin was compared with isolated keratinocytes grown in a conventional tissue culture system . ^^^ TNF also induced keratinocyte ICAM 1 expression ( although to a lesser degree than IFN gamma ) but did not induce either keratinocyte HLA DR or DQ expression . ^^^ There was good correlation of keratinocyte expression of ICAM 1 and HLA DR by IFN gamma and TNF when the epidermis of the organ culture system was compared with the isolated keratinocytes grown in tissue culture . ^^^ Characterization of intercellular adhesion molecule 1 and HLA DR expression in normal and inflamed skin : modulation by recombinant gamma interferon and tumor necrosis factor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , after a 72 hr exposure of islets to interferon gamma ( IFN gamma ) and / or tumor necrosis factor alpha ( TNF alpha ) ( each at 250 units / ml ) , ICAM 1 was induced on greater than 85 % of islet cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Plasma samples obtained from patients receiving a 24 h continuous infusion of human recombinant ( hr ) TNF or a combination of two overlapping , 24 h continuous infusions of hrIFN gamma and hrTNF were analyzed for IL 6 in a sensitive bioassay . ^^^ Circulating interleukin 6 during a continuous infusion of tumor necrosis factor and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The influence of recombinant ( r ) human tumor necrosis factor alpha ( rTNF alpha ) , r human interleukin 1 beta ( rIL 1 beta ) , and r human interferon gamma ( rIFN gamma ) on the production of interleukin 6 ( IL 6 ) by human endothelial cells ( HEC ) was investigated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The mechanisms of lymphoma inhibition by TNF or IFN gamma are discussed . . ^^^ Tumor necrosis factor and interferon gamma inhibit the development of radiation induced thymic lymphomas in C57BL / Ka mice . ^^^ Recombinant tumor necrosis factor and / or gamma interferon were injected into C57BL / Ka mice after completion of a whole body split dose irradiation , which usually induces thymic lymphomas in more than 90 % of the animals . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Monoclonal antibody against interferon gamma can prevent experimental cerebral malaria and its associated overproduction of tumor necrosis factor . ^^^ Injections of neutralizing monoclonal antibody against recombinant murine interferon gamma , not later than 4 days after infection , markedly reduced the incidence of ECM and the elevation in serum levels of tumor necrosis factor . ^^^ It is proposed that interferon gamma released by activated CD4+ T cells acts by augmenting both production and action of tumor necrosis factor . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , Go does not appear to be obligatory for the common pathway of induction of HL 60 differentiation , because PT showed a little or no effect on differentiation induced by other inducers such as 1 alpha , 25 dihydroxyvitamin D 3 , tumor necrosis factor , interferon gamma , lymphotoxin , prostaglandin E 2 , cholera toxin , and dimethylsulfoxide . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Activation of human peripheral blood derived monocytes by OK 432 ( Streptococcus pyogenes ) : augmented cytotoxicity and secretion of TNF and synergy with rIFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Synergistic activity of tumor necrosis factor and interferon in a nude mouse model of human ovarian cancer . ^^^ Animals were treated by intraperitoneal injection with ( 1 ) recombinant human tumor necrosis factor alpha ( rHTNF ) 5 or 10 micrograms / day ; ( 2 ) recombinant murine interferon gamma ( rMIFN ) 5 10 10 ( 4 ) units per day ; or ( 3 ) a combination of rHTNF 5 micrograms / day and rMIFN 5 10 10 ( 4 ) units / day . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of the tumor necrosis factor ( TNF ) , and a second pleiotropic cytokine interferon gamma ( IFN ) , were examined in a line of human myeloblastic leukemia cells ( ML 1 ) . ^^^ Differentiation inducing and cytotoxic effects of tumor necrosis factor and interferon gamma in myeloblastic ML 1 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The combination of tumor necrosis factor ( TNF ) and interferon gamma has synergistic bioactivity in numerous preclinical model systems . ^^^ Thirty six patients were treated with a fixed dose of interferon gamma ( 200 micrograms / m2 / d ) with interpatient dose escalation of TNF ( from 5 to 205 micrograms / m2 / d ) . ^^^ The dose limiting toxicity at the maximal tolerated dose ( MTD ) of TNF ( 205 micrograms / m2 ) with interferon gamma was hypotension . ^^^ The recommended phase 2 dose for this combination schedule is TNF , 136 micrograms / m2 , with interferon gamma , 200 micrograms / m2 . ^^^ The addition of interferon gamma to TNF resulted in a greater than three fold increase in toxicity compared with TNF administered as a single agent , supporting the hypothesis that the combination of these cytokines may induce synergistic effects in vivo . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cooperative effects of recombinant human tumor necrosis factor ( rH TNF ) and recombinant human interferon gamma ( rH IFN gamma ) against the proliferation of four human gynecologic tumor cell lines ( HHUA , ISHIKAWA , HeLa S 3 , and SCH ) were examined in vitro . ^^^ The effect of rH IFN gamma on TNF receptors was also examined . ^^^ A synergistic antiproliferative effect of rH TNF and rH IFN gamma appeared in HHUA , ISHIKAWA , and HeLa S 3 cells . ^^^ The effectiveness of rH TNF increased in these cell lines pretreated with rH IFN gamma , but not vice versa . rH IFN gamma did not change the binding affinity of TNF receptors in the four cell lines , but increased the number of binding sites for TNF in HeLa S 3 and SCH cells . ^^^ These findings suggest that there is no relationship between the increase in TNF binding sites by rH IFN gamma and the synergistic antiproliferative effect of rH IFN gamma and rH TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In 2 of the 4 patients , 4 types of cytokines ( tumor necrosis factor , interleukin 1 beta , interleukin 2 , and interferon gamma ) were induced in ascites after intraperitoneal injection of OK 432 ( i . p . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have investigated the effect of endogenous production of tumor necrosis factor ( TNF ) induced by the combination of recombinant interferon gamma ( rIFN gamma ) as a primer followed by GLA 60 as a trigger ( rIFN gamma / GLA 60 ) on murine lung metastases caused by B 16 BL6 melanoma . ^^^ In order to examine the therapeutic effect of endogenous TNF on tumor metastasis , the ability of multiple administrations of rIFN gamma / GLA 60 to induce TNF production was also tested . ^^^ The multiple administrations of rIFN gamma / GLA 60 at intervals of 2 days were effective for the induction of endogenous TNF in mice but continuous multiple administrations of them for 2 4 days were not . ^^^ In tumor bearing mice , the production of endogenous TNF by rIFN gamma / GLA 60 was less than that of normal mice , but treatment 3 days after the surgical excision of primary tumors showed the endogenous TNF production to be similar to that in normal mice . ^^^ In the experimental lung metastasis model , intravenous administration of rIFN gamma followed by intravenous or intranasal administration of GLA 60 showed potent inhibition of lung metastases of B 16 BL6 melanoma , whereas the reverse sequence of administration ( GLA 60 / rIFN gamma ) or administration of a mixture of rIFN gamma and GLA 60 , which can not induce the production of TNF , caused no inhibition of lung metastases . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of biologic response modifiers such as interferon gamma , tumor necrosis factor alpha ( TNF ) , and retinoic acid on the human chorionic gonadotropin ( hCG ) secretion of cultured choriocarcinoma cells ( JAR ) and term placenta have been studied . ^^^ In the case of the term placenta , only retinoic acid increased the hCG secretion into the culture medium , whereas interferon gamma and TNF both markedly reduced secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Control of lymphoepithelial interactions within thymic nurse cells by gamma interferon and tumor necrosis factor alpha . ^^^ The results presented in this paper demonstrate that interferon gamma and tumor necrosis factor alpha regulate ' in vivo ' and ' in vitro ' the interactions between epithelial cells and thymocytes within thymic nurse cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Recombinant human interleukin 1 beta ( rhIL 1 beta ) and interferon gamma ( rhIFN gamma ) were not cytocidal , and did not synergize with recombinant human TNF ( rhTNF ) in the L 929 cell bioassay . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) enhanced the IL 2 dependent proliferation of TIL ' s cultured in low concentrations of IL 2 ( 10 U / ml ) ; however , neither TNF alpha nor interferon gamma was able to reduce the inhibitory effect of TGF beta 2 on TIL proliferation . ^^^ Tumor necrosis factor alpha ( TNF alpha ) enhanced the IL 2 dependent proliferation of TIL ' s cultured in low concentrations of IL 2 ( 10 U / ml ) ; however , neither TNF alpha nor interferon gamma was able to reduce the inhibitory effect of TGF beta 2 on TIL proliferation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We provide evidence that CPZ inhibits the accumulation of mRNA specific for the lymphokines , interleukin 2 , interferon gamma , tumor necrosis factor alpha and the proto oncogene c myc ; by contrast , the accumulation of mRNA specific for the alpha chain of the interleukin 2 receptor and the subsequent early expression of Tac antigen on the cell surface is not inhibited by CPZ . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The mechanism by which LiCl increases the cytotoxic response seems to differ from the sensitizing effect of actinomycin D or interferon gamma , since the latter treatments overcame TNF resistance of several cell lines , whereas LiCl did not . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A phase I / II study of recombinant tumor necrosis factor and recombinant interferon gamma in patients with AIDS related complex . ^^^ A clinical trial was conducted to determine the tolerance and toxicity of recombinant tumor necrosis factor ( rTNF ) and recombinant interferon gamma ( rIFN gamma ) when administered concurrently by continuous intravenous infusion to 11 patients with the AIDS related complex ( ARC ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The clonal proliferation of human bone marrow cells in response to IL 3 was inhibited by tumor necrosis factor and by lymphotoxin , but not by interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In our hands , IL 1 , IL 2 , granulocyte / macrophage colony stimulating factor , tumor necrosis factor , interferon gamma , and IL 5 tested over a wide concentration range gave very weak chemotaxis for eosinophils in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Recombinant granulocyte macrophage colony stimulating factor ( GM CSF ) , recombinant interleukin 1 ( alpha and beta ) , recombinant interferon gamma , recombinant tumor necrosis factor or combinations of the latter two failed to up regulate tumor cell sensitivity to NK and LAK cell cytotoxicity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Acivicin potentiated the differentiating effects of interferon gamma , tumor necrosis factor , dihydroxyvitamin D 3 , dimethylsulfoxide , and retinoic acid . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In separate experiments we were able to show that the number of TNF alpha binding sites on both rHuTNF alpha sensitive and resistant ME 180neo cells was similar and was increased by treatment with rHuIFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These findings suggest that 1 ) in addition to direct cytopathic effects , reovirus infection may contribute to beta cell destruction by increasing expression of class 1 MHC proteins and therefore reactivity with cytotoxic T lymphocytes ; and 2 ) some viruses may increase MHC protein expression independent of and before the action of cytokines ( e . g . , interferon gamma and tumor necrosis factor ) released by immunoinflammatory cells . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated whether this synergism could be explained by the presence of an increased number of TNF receptors in cells treated with rIFN gamma . ^^^ The treatment with rIFN gamma did not change the binding affinity of TNF receptors , but increased their number to 1800 per cell from a basal level of about 800 per cell . ^^^ A significant increase in TNF receptors after treatment with rIFN gamma was observed in HeLa D98 / AH2 , but not in SK MEL 109 cells . ^^^ These results indicate that the synergism between rTNF and rIFN gamma may be due , at least in part , to a transient induction of the synthesis of TNF receptors by rIFN gamma in cells with a relatively low number of these receptors . . ^^^ Induction of the synthesis of tumor necrosis factor receptors by interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We also demonstrated that the IL 2 / TNF synergistic induction of LAK activity did not involve either IL 1 or interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma , tumor necrosis factor , GM CSF , interleukin 1 and interlukin 4 synergistically enhanced the differentiation of U 937 cells when combined with F DIF . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Recent studies suggest that TNF is also implicated : the gene encoding TNF is situated close to the MHC gene in both mice and humans , and TNF is able to up regulate constitutively expressed class 2 antigen and , with interferon gamma , to induce class 2 expression in previously normal cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Previous studies in this laboratory have demonstrated that the adhesion of T lymphocytes to endothelial cell ( EC ) monolayers in vitro can be increased by preincubation of the EC with interferon gamma , interleukin 1 ( IL 1 ) , tumor necrosis factor alpha ( TNF ) , or lipopolysaccharide ( LPS ) , or by stimulation of the T cells with phorbol esters . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Additionally , recombinant DNA technology has facilitated the economic production of rare lymphokines ( e . g . , interleukin 2 , interferon alpha and interferon gamma ) or cytokines ( tumor necrosis factor , lymphotoxin ) that can either modulate the host immune response or kill tumor cells , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Other immunomodulators , including interleukin 1 , interleukin 2 , interferon gamma , tumor necrosis factor , or granulocyte CSF ( G CSF ) , had no effect upon microglial growth in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The influences of human tumor necrosis factor ( TNF ) ( LuKII ) , recombinant human TNF alpha , natural human interferon gamma ( HuIFN gamma ) , recombinant HuIFN gamma , and natural HuIFN alpha were evaluated alone or in combination for their effects in vitro on colony formation by human bone marrow granulocyte macrophage ( CFU GM ) , erythroid ( BFU E ) , and multipotential ( CFU GEMM ) progenitor cells incubated at 5 % CO 2 in lowered ( 5 % ) O 2 tension . ^^^ When combinations of TNF and HuIFN were evaluated together , it was noted that TNF ( LuKII ) or recombinant TNF synergized with natural or recombinant HuIFN gamma , but not with HuIFN alpha , to suppress colony formation of CFU GM , BFU E , and CFU GEMM from bone marrow of normal donors at concentrations that had no suppressive effects when molecules were used alone . ( ABSTRACT TRUNCATED AT 400 WORDS ) . ^^^ The suppressive influences of human tumor necrosis factors on bone marrow hematopoietic progenitor cells from normal donors and patients with leukemia : synergism of tumor necrosis factor and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
DNA fragmentation and cytotoxicity caused by tumor necrosis factor is enhanced by interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma and lymphotoxin or tumor necrosis factor act synergistically to induce macrophage killing of tumor cells and schistosomula of Schistosoma mansoni . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When recombinant human interferon gamma ( rIFN gamma ) is added to cultured cells , TNF production is increased . rIFN gamma is also able to sustain the elevated production level of TNF over a 4 day period . ^^^ Recombinant interferon alpha ( rIFN alpha ) was unable to stimulate increased TNF production . ^^^ Recombinant interferon gamma stimulates the production of human tumor necrosis factor in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma treatment of endothelial cells and fibroblasts had no direct effect on membrane IL 1 expression and little effect when used as a pretreatment for TNF or lipopolysaccharide stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma , interferon alpha / beta , and tumor necrosis factor differentially affect major histocompatibility complex class 1 expression in murine leukemia virus induced tumor cell lines . ^^^ Culturing these cells with interferon ( IFN ) gamma , IFN alpha / beta , or tumor necrosis factor increases both K and D expression in these cell lines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Anti tumor activity of recombinant mouse tumor necrosis factor ( TNF ) on colon cancer in rats is promoted by recombinant rat interferon gamma ; toxicity is reduced by indomethacin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma requires tumor necrosis factor to restrain tumor cell growth in peritoneal cell cultures from tumor dormant mice . ^^^ Treatment of these PC cultures with ' antibodies to murine interferon gamma ( MuIFN gamma ) and murine tumor necrosis factor ( MuTNF ) but not with antibody to interleukin 2 ( IL 2 ) receptors eliminated the restraint on tumor cell growth and permitted their progressive proliferation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Since interferon gamma activates macrophages to release reactive oxygen species , and tumor necrosis factor alpha ( TNF alpha ) helps both to mediate endotoxicity and to induce leukocytes to secrete reactive oxygen , we monitored the effects of administering recombinant forms of these cytokines on Plasmodium chabaudi adami infections in mice . ^^^ Infections were suppressed by daily i . p . injections of 5 10 10 ( 4 ) U of recombinant mouse interferon gamma from day 1 or by recombinant human TNF released from i . p . osmotic pumps at the rate of 6 10 10 ( 3 ) U / hr . ^^^ Inhibition of murine malaria ( Plasmodium chabaudi ) in vivo by recombinant interferon gamma or tumor necrosis factor , and its enhancement by butylated hydroxyanisole . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytotoxicity of tumor necrosis factor for thyroid epithelial cells and its regulation by interferon gamma . ^^^ The increased susceptibility of the cells to the cytotoxicity of tumor necrosis factor was clear as early as 2 4 h after the addition of interferon gamma and greatly preceded the induced expression of major histocompatibility complex class 2 antigen . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro growth inhibition of cisplatin resistant human lung cancer cell lines by recombinant human tumor necrosis factor and / or recombinant human interferon gamma by virtue of collateral sensitivity . ^^^ The colony inhibitory effects of recombinant human tumor necrosis factor ( rH TNF ) and recombinant human interferon gamma ( rH IFN gamma ) were evaluated in four human lung cancer cell lines and their cisplatin resistant sublines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma synergizes with tumor necrosis factor and with interleukin 1 and requires the presence of both monokines to induce antitumor cytotoxic activity in macrophages . ^^^ Small concentrations of recombinant murine interferon gamma ( MuIFN gamma ) , recombinant human interleukin 1 ( HuIL 1 ) , and recombinant murine tumor necrosis factor ( MuTNF ) , added separately to cultures of thioglycolate elicited peritoneal macrophages , produced no cytotoxic activity against L5178Y cells , a tumor cell line which is resistant to the direct toxic effects of these cytokines , either alone or in combination . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These results suggest that the transient release of cachectin / TNF , followed by interleukin 1 and interferon gamma , may participate in the cascade of events noted in overwhelming bacterial invasion . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor enhances interferon induced Ia antigen expression on murine islet parenchymal cells . ^^^ BR mice ( H 2k ) were isolated by the collagenase technique , hand picked with a Pasteur pipette , and incubated in tissue culture media supplemented with recombinant murine interferon gamma ( rIFN gamma ) , recombinant murine tumor necrosis factor ( rTNF ) , or both . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human promyelocytic leukemia cells ( HL 60 ) differentiate along a monocytoid pathway in response to recombinant human tumor necrosis factor or recombinant human interferon gamma . ^^^ Since reduced expression of mRNA for the proto oncogene c myc correlates with differentiation of HL 60 cells induced by other agents , we tested the abilities of tumor necrosis factor and interferon gamma to regulate expression of c myc mRNA . ^^^ In contrast , interferon gamma did not affect c myc mRNA levels , nor did it show synergy with tumor necrosis factor in reducing c myc . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We avoided confusion with tumor necrosis factor effects by using recombinant human interferon gamma cloned from a single gene as opposed to natural human interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although with different magnitude , recombinant interferon alpha A / D ( Bgl 2 ) hybrid , interferon gamma and tumor necrosis factor , all increase the activity of this enzyme , whereas interleukin 1 , recombinant interferon alpha A and interferon alpha D do not induce this activity in mice lung tissue . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Induction of interferon gamma and tumor necrosis factor in ascites of a patient with ovarian cancer by OK 432 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Augmentation of cytotoxic activity of recombinant human tumor necrosis factor ( rHu TNF ) by recombinant human interferon gamma ( rHu IFN gamma ) . ^^^ The present study was undertaken to examine the effect of recombinant human interferon gamma ( rHu IFN gamma ) on the in vitro antitumor activity of recombinant human tumor necrosis factor ( rHu TNF ) against rHu TNF sensitive and resistant tumor cells . rHu IFN gamma augmented both cytostatic and cytocidal activity of rHu TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found that interferon gamma completely abolished bone resorption stimulated by the cytokines interleukin 1 , tumor necrosis factor alpha and tumor necrosis factor beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although single agent interferon regimens for advanced malignant melanoma will probably not offer a substantive therapeutic advance , combinations of these molecules with other biological agents ( tumor necrosis factor ) , biochemical modulators ( difluoromethylornithine ) , and cytotoxic agents ( bischloroethylnitrosourea , BCNU ) offer innovative therapeutic dimensions in the design of future clinical investigations . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Sera from 3 renal allograft recipients treated with OKT 3 were studied and showed that a massive although transient release of some cytokines namely , Tumor Necrosis Factor alpha , Interleukin 2 and Interferon gamma is observed following the first OKT 3 injection . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have carried out a longitudinal study of interferon ( IFN ) and tumor necrosis factor ( TNF ) using a whole blood mitogen stimulation assay in 20 multiple sclerosis ( MS ) patients and in a healthy control group . ^^^ Before exacerbations , however , we found an increase of IFN gamma and TNF production preceding clinical symptoms by a maximum of 2 weeks . ^^^ It may be that IFN gamma and TNF trigger off exacerbations at a very early stage and that these cytokines may also play a role in maintaining disease in chronic progressive and invalidating forms . . ^^^ Increased production of interferon gamma and tumor necrosis factor precedes clinical manifestation in multiple sclerosis : do cytokines trigger off exacerbations . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The assay is highly specific for IFN gamma : there is no cross reaction either with interferons alpha and beta , Interleukins 1 and 2 , tumor necrosis factor alpha and beta or with various brain peptides . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Production of tumor necrosis factor ( TNF ) by monocytes from cancer patients and healthy subjects induced by OK 432 in vitro , and its augmentation by human interferon gamma . ^^^ Recombinant human gamma interferon ( IFN gamma ) alone did not induce TNF as described in other reports , but enhanced the induction of TNF by OK 432 when added to monocytes before or with OK 432 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Maximal PAF induced cytotoxicity was 68 + / 6 % , which was similar to that induced by optimal concentrations of tumour necrosis factor ( TNF ) and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When HEC were treated with cytokines known to modulate a number of specialized functions of these cells , we observed that , unlike interferon gamma , interleukin 1 ( IL 1 ) and tumor necrosis factor ( TNF ) were able to induce appreciable levels of c fos in HEC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor , interferon gamma and interleukin 2 ( IL 2 ) had no effect on IL 6 synthesis in monocytes or macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Because IL 2 fails to induce the same antigens on cultured endothelial cells , we infer that IL 2 acts in vivo by inducing the production of other cytokines ( e . g . , interleukin 1 , tumor necrosis factor , lymphotoxin , and interferon gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Known activators or mediators of macrophage cytotoxicity ( endotoxin , interferon gamma , tumor necrosis factor ) did not replace L arginine for CM mediated fungistasis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To study possible modulatory effects of other cytokines on IL 1 mediated beta cell cytotoxicity , we added human recombinant IL 1 alpha and beta ( rIL 1 alpha , rIL 1 beta ) , tumor necrosis factor ( rTNF ) , lymphotoxin ( rLT ) , and interferon gamma ( rIFN gamma ) separately or in combinations to the culture medium of isolated rat islets of Langerhans . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Also inactive as inducers of HUVEC Mo3e expression were crude lymphokine and monokine supernatants , recombinant human lymphokines ( interferon gamma and interleukin 2 ) , recombinant human monokines ( interleukin 1 and tumor necrosis factor ) , bacterial cell wall products including LPS and MDP , pharmacologic agents that increase intracellular cyclic adenosine monophosphate ( prostaglandin E 2 , cholera toxin , theophylline , isoproterenol and isobutylmethylxanthine ) , lectins ( Con A and PHA ) , and heparin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , we find that lymphotoxin , a cytokine functionally and genetically related to TNF , also synergized with the bacteria to cause HN , whereas interleukin 1 alpha or interferon gamma did not . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
DIF and interferon gamma ( gamma IFN ) were shown to be separate molecules inasmuch as a neutralizing antibody for gamma IFN did not abolish the DIF effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The peptide was not antigenically similar to interferon gamma , tumor necrosis factor , or interleukin 1 alpha or 1 beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have identified one of these factors as tumor necrosis factor alpha ( TNF alpha ) , and have shown that production of this factor is enhanced by recombinant human interferon gamma ( rHuIFN gamma ) . ^^^ Furthermore , NK cell mediated cytotoxicity is not reliably inhibited by antibodies directed against various epitopes of recombinant human TNF alpha and / or recombinant TNF beta ( lymphotoxin ) or rHuIFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast to recombinant interferon gamma ( rIFN gamma ) , the following factors , tested in partially or highly purified form and over a wide range of concentrations , did not augment these functions : native interferon alpha ( nIFN alpha ) , rIFN alpha A , rIFN alpha D , rIFN beta , colony stimulating factor ( type 1 ) ( CSF 1 ) , CSF for granulocytes and macrophages ( GM CSF ) , pluripotent CSF ( p CSF ) , tumor necrosis factor ( TNF ) , native interleukin 2 ( nIL 2 ) , and rIL 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of uridine phosphorylase mRNA in HCT 116 cells was further enhanced by treating the cells with vitamin D 3 and the inflammatory cytokines : tumor necrosis factor alpha , interleukin 1 alpha and interferon gamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Differential effects of interferon gamma and beta on fatty acid turnover , lipid bilayer fluidity and TNF alpha release in murine macrophage J774 . 2 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
SEA , SEB , SEC and TSST 1 , all induced the in vitro release of interleukin 2 , interferon gamma and tumor necrosis factor alpha in a dose dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of mRNA for 55 kDa and 75 kDa tumor necrosis factor ( TNF ) receptors in mouse cerebrovascular endothelium : effects of interleukin 1 beta , interferon gamma and TNF alpha on cultured cells . ^^^ Interferon gamma and interleukin 1 beta up regulated the levels of both the 55 kDa and 75 kDa TNFr mRNA , while TNF alpha had no effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Concentrations of two immune cytokines , namely interferon gamma ( INF gamma ) and tumor necrosis factor alpha ( TNF alpha ) , were determined in the sera and cervical mucus samples of fertile ( n = 16 ) , idiopathic infertile ( n = 44 ) , and immunoinfertile women ( n = 45 ) to investigate their role , if any , in female infertility . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In previous studies , HL 60 AML cells treated with tumor necrosis factor alpha ( TNF ) , interferon gamma ( IFN ) , and lipopolysaccharides ( LPS ) displayed decreased growth and viability , enhanced monocytic pathway differentiation and endogenous TNF release . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma induced a weak increase in VCAM 1 mRNA expression , with no synergistic effect on the stimulation by TNF alpha . ^^^ The addition of interferon gamma delays the time at which peak expression of ICAM 1 in response to TNF alpha stimulation occurs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro exposure of murine endothelial cells to various cytokines ( interferon gamma , tumor necrosis factor alpha , and interleukin 1 alpha or 1 beta ) resulted in their activation to kill schistosomula through an arginine dependent mechanism involving production of nitric oxide ( NO ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines , including interferon gamma ( IFN ) , IL 3 and granulocyte macrophage colony stimulating factor ( GM CSF ) , amplify the inflammatory response by increasing production of IL 1 and TNF by macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The levels of tumour necrosis factor alpha ( TNF alpha ) , neopterin , interleukin 2 receptor and granulocyte macrophage colony stimulating factor were higher in patients with spontaneous bacterial peritonitis , but without statistical significance , whereas no differences were found between the interferon gamma , interleukin 2 and interleukin 1 levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1 beta , TNF alpha , and LPS each induced NO synthase activity , assessed by release of nitrite , conversion of L arginine to L citrulline , and increased levels of guanosine 3 ' , 5 ' cyclic monophosphate , whereas IL 2 , IL 6 , and interferon gamma were ineffective . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human recombinant IL 10 suppressed interferon gamma induced major histocompatibility complex class 2 up regulation in rat peritoneal macrophages , exhibited pleiotropic effects on thymocytes and totally abrogated tumor necrosis factor production of encephalitogenic T lymphocytes in vitro , without simultaneously affecting proliferative responses of the cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines such as interleukin 1 , tumor necrosis factor , and interferon gamma have been shown to stimulate NO production in many cells types . ^^^ NO production was not significantly altered by PTH and 1 , 25 dihydroxyvitamin D or human recombinant interleukin 1 beta ( 10 U / ml ) , tumor necrosis factor alpha ( 25 ng / ml ) , and interferon gamma ( 100 U / ml ) individually . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , cytokine production of the gamma delta T cell lines was similar to the Th 1 pattern [ interferon gamma , tumor necrosis factor ( TNF ) alpha and TNF beta ] . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The latter cell type does not express NO synthase normally but expression is induced by the cytokines , interferon gamma , tumor necrosis factor , and interleukin 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma increased immunologically reactive major histocompatibility complex class 2 antigens ( < 300 to 2 , 398 + / 225 average fluorescence intensity ) , while tumor necrosis factor alpha induced an increase in vascular cell adhesion molecule 1 ( 2 , 167 + / 171 ) and E selectin ( 1 , 628 + / 315 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Rat hepatocytes were plated at densities from 10 ( 4 ) to 20 10 10 ( 4 ) hepatocytes / cm2 and stimulated with a combination of LPS , interferon gamma , interleukin 1 , and tumor necrosis factor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the presence of ATRA , GM CSF potentiated production and secretion of tumor necrosis factor alpha ( TNF ) in response to lipopolysaccharide , as well as interferon gamma which is a potent inducer of monocytic differentiation in APL cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Activation of CD56dim NK cells by IL 15 was similar to that of IL 2 as measured by enhanced NK cytotoxic activity , antibody dependent cellular cytotoxicity , and NK cell production of interferon gamma , tumor necrosis factor alpha , and granulocyte / macrophage colony stimulating factor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Nitric oxide production by epithelial cells was enhanced by culture in the presence of interferon gamma , interleukin 1 beta , tumor necrosis factor alpha , and lipopolysaccharide ; the NOS activity expressed under these conditions showed less dependence on calcium , reminiscent of other inducible forms of NOS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The level of constitutive expression attained in the transgenic mice exceeded the peak level of ICAM 1 expression induced on nontransgenic mouse keratinocytes in vitro by optimal combinations of interferon gamma and tumor necrosis factor alpha or in vivo by proinflammatory stimuli such as phorbol 12 myristate 13 acetate . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neither lipopolysaccharide ( LPS ) , interleukin 1 alpha ( IL 1 ) , tumor necrosis factor alpha ( TNF ) , nor interferon gamma ( IFN ) was able to stimulate nitrite production by C2C12 cells when administered alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
All four T cell lines produced roughly equivalent amounts of interferon gamma , lymphotoxin / tumor necrosis factor ( TNF alpha / beta ) , interleukin 3 , interleukin 6 and transforming growth factor beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We evaluated the levels of expression and types of TNF alpha receptor expressed by the cell line , and the cell lines response to interleukin 4 and interferon gamma . ^^^ However , interferon gamma is able to synergize with TNF to enhance the expression of E selectin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CTL clones reactive to MBP 110 118 and MAG 556 564 produced tumor necrosis factor alpha and a subset of these clones also produced interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Effects of interferon gamma , tumor necrosis factor alpha , and estrogen . ^^^ Increased expression of ICAM 1 by interferon gamma and tumor necrosis factor alpha alone , and the further induction in combination with beta estradiol may underlie an aspect of fibroblast dysfunction in SSc and the female predisposition to the disease . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Nitric oxide synthase ( NOS ) induction by tumor necrosis factor alpha ( TNF alpha ) is synergized by interferon gamma , and both NOS activity and gene expression are maximal by 10 h and maintained through 24 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Bovine macrophage derived tumour necrosis factor alpha / cachectin ( TNF alpha ) was synthesized when peripheral blood mononuclear cells ( PBMC ) and purified adherent PBMC from naive and Theileria annulata infected cattle were incubated in vitro with concanavalin A ( Con A ) or bovine recombinant interferon gamma ( Bo rIFN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increasing evidence indicates that the cytokines , tumor necrosis factor ( TNF ) , interleukin 1 , and / or interferon gamma , may play a crucial role in the pathogenesis of multiple sclerosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Induction of nitrite / nitrate production by the combination of tumor necrosis factor alpha and bacterial lipopolysaccharide or that of interleukin 1 alpha and interferon gamma ( 100 U / mL ) was also inhibited by cyclosporin A cotreatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Under basal conditions , the production of nitrite , a stable metabolite of NO , was negligible ; however , incubation with tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IF gamma ) for 24 h resulted in a 12 fold increase in nitrite synthesis and the appearance of abundant iNOS mRNA and protein . ^^^ Nuclear run on transcription demonstrated that the iNOS gene was transcriptionally inactive under basal conditions , but was markedly induced by TNF alpha and IF gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The level of expression on EC is increased about 3 fold following 24 h treatment with optimal concentrations of tumor necrosis factor , interleukin 1 , interferon beta , or interferon gamma ; both interferons show greater than additive induction of CD 40 when combined with tumor necrosis factor or interleukin 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Horse trophoblasts produce tumor necrosis factor alpha but not interleukin 2 , interleukin 4 , or interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Collective data suggest that certain cytokines associated with the TH 1 phenotype or lymphocytes , such as tumor necrosis factor alpha , lymphotoxin , interleukin 12 , and interferon gamma , may promote disease , while cytokines produced by the TH 2 subset , such as interleukin 10 , may limit disease . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytometric analysis revealed that , in basal conditions , low levels of ICAM 1 and VCAM 1 were present on the surface of the cells , whereas treatment with interleukin 1 , tumor necrosis factor alpha , lipopolysaccharide , or interferon gamma induced , with different kinetics , increases in their expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Hepatocytes isolated from endotoxemic rats released increased amounts of nitric oxide , measured by nitrite production , in response to interferon gamma ( gamma IFN ) alone or in combination with LPS , tumor necrosis factor alpha , macrophage colony stimulating factor , granulocyte / macrophage colony stimulating factor , or hepatocyte growth factor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When VSMC were stimulated with a combination of LPS , interferon gamma ( INF ) and tumor necrosis factor ( TNF ) nitrite production was 5 fold greater than with LPS alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pretreatment with intratracheal interferon gamma 24 h before intratracheal LPS increased TNF bioactivity partly due to increased TNF mRNA and by increasing TNF processing , as evidenced by a decrease in the 26 kDa TNF prohormone and an increase in immunoreactive and bioactive TNF . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Because neovascularization may require various proteinases , we characterized the profile of metalloenzyme production by microvascular endothelial cells ( MEC ) and the modulation of expression by phorbol esters ( PMA ) and by the physiologically relevant cytokines tumor necrosis factor alpha ( TNF alpha ) , basic fibroblast growth factor , and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon alpha , interferon gamma , tumor necrosis factor , transforming growth factor beta , all trans retinoic acid and cisplatin did not affect CD44v expression . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study , we demonstrate that a combination ( CL ) of interleukin 1 alpha , interferon gamma , tumor necrosis factor alpha and lipopolysaccharide induces nitrite ( NO 2 ) production in cultured rat Sertoli cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The activity of TNF or anti TNFR 80 was selective for NK cytotoxic function because they did not directly mimic IL 2 activation or induce significant proliferation , expression of cell surface activation antigens ( CD 25 or HLA DR ) , or interferon gamma secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha induced an increase in PAP 1 mRNA expression , and interferon gamma caused an even greater increase in PAP 1 mRNA level . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
During an early reversible allograft rejection episode , both T cell associated [ interleukin ( IL ) 2 , IL 2 receptor , IL 4 , and interferon gamma ] and macrophage ( IL 1 alpha , tumor necrosis factor alpha , and IL 6 ) products were up regulated despite transient immunosuppression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunohistochemical analysis showed strong production of the tumoricidal cytokines tumor necrosis factor alpha and interferon gamma in the tumor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When murine peritoneal macrophages were loaded in vitro with Plasmodium vinckei hemozoin and stimulated for 24 hours with interferon gamma and / or Escherichia coli lipopolysaccharide , the production of interleukin 6 ( IL 6 ) was drastically reduced , whereas the secretion of tumor necrosis factor ( TNF ) was increased . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine assays revealed the production of interleukin 2 , interferon gamma , tumor necrosis factor and granulocyte macrophage colony stimulating factor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In view of the importance of basic fibroblast growth factor as a mitogenic and differentiating agent for vascular endothelial cells , various growth factors were tested ( acidic fibroblast growth factor , epidermal growth factor , platelet derived growth factor , transforming growth factors alpha and beta , vascular endothelial growth factor , interferon gamma , tumor necrosis factor , insulin , and interleukin 4 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma and tumor necrosis factor alpha mRNA expression in mycosis fungoides progression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The pathogenetic and antiviral potential of the cytotoxic T lymphocyte ( CTL ) response to HBV has been demonstrated by the induction of a severe necroinflammatory liver disease following the adoptive transfer of HBsAg specific CTL into HBV transgenic mice , and by the noncytolytic suppression of viral gene expression and replication in the same animals by a posttranscriptional mechanism mediated by interferon gamma , tumor necrosis factor alpha , and interleukin 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Salivary gland extracts collected daily during engorgement were shown to inhibit normal murine macrophage elaboration of interleukin 1 ( IL 1 ) and tumor necrosis factor alpha ( TNF ) as well as murine T lymphocyte production of interleukin 2 ( IL 2 ) and interferon gamma ( IFN G ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Middle ear effusions were cultured for bacterial pathogens and assayed for interleukin 1 beta , interleukin 6 , tumor necrosis factor alpha , and interferon gamma . ^^^ RESULTS : Interleukin 1 beta was detected in 58 % ( 44 / 75 ) MEEs ; interleukin 6 , 83 % ( 60 / 72 ) ; tumor necrosis factor alpha , 37 % ( 28 / 75 ) [ corrected ] ; and interferon gamma , 61 % ( 45 / 74 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lymphocyte cultures that displayed increases in cytotoxicity after stimulation with the gamma IFN transduced melanoma cells also exhibited enhanced expression or induction of one or more of the following lymphokines : IL 4 , IL 1 alpha , IL 1 beta , gamma IFN , and TNF alpha . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF ) and interferon gamma ( IFN ) modulate immune responses , as TNF is postulated as a first messenger for priming immune cells and IFN as the second messenger for activation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MEASUREMENTS AND MAIN RESULTS : Therapy with monoclonal anti TNF alpha antibodies prevented the posthemorrhage increases in pulmonary TNF alpha and interferon gamma protein levels that normally occur after blood loss . ^^^ Administration of monoclonal anti TNF alpha antibodies also diminished the increases in interleukin 1 beta , interleukin 6 , and interleukin 10 mRNA , but not the increases in TNF alpha and interferon gamma mRNA , which are found in the lungs following hemorrhage . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The enhancement of collagenase gene expression by TNF R 55 specific TNF alpha was augmented by simultaneous treatment of normal and scleroderma skin fibroblasts with interferon gamma , indicating specific enhancement of TNF R 55 signaling pathway by interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of human endothelial cells with cytokines such as interleukin 1 , tumor necrosis factor alpha ( TNF alpha ) or interferon gamma induces the expression of specific leukocyte adhesion molecules on the endothelial cell surface . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokines , including IL 1 , IL 2 , IL 6 , interferon gamma , and tumor necrosis factor , exert profound effects on hypothalamic pituitary axes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Established RCC tumor cell lines derived from these RCC patients were negative for interleukin 2 ( IL 2 ) , IL 4 , IL 10 , and interferon gamma and found to be positive for tumor necrosis factor alpha ( TNF alpha ) , IL 6 , IL 1 beta , granulocyte / macrophage colony stimulating factor ( GM CSF ) , and transforming growth factor beta 1 ( TGF beta 1 ) message as detected by PCR . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The present report describes the clinical course of this patient and examines the contribution of production of tumor necrosis factor ( TNF ) and interferon gamma ( IFN ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These CD4+ CTL clones produced several activating cytokines including tumor necrosis factor alpha , interferon gamma , granulocyte macrophage colony stimulating factor ( GM CSF ) , and macrophage CSF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of CD 34 at the antigen and mRNA levels on early passage cells is rapidly downregulated by interleukin 1 beta ( IL 1 beta ) , interferon gamma ( INF gamma ) , and tumor necrosis factor alpha ( TNF alpha ) under conditions in which these ligands upregulate the adhesion molecules : endothelial leukocyte adhesion molecule 1 ( ELAM 1 ) and intracellular adhesion molecule 1 ( ICAM 1 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It was induced / enhanced on dense and buoyant tonsillar B cells , respectively , through surface Ig cross linking in combination with interleukin 2 or by interferon gamma together with tumor necrosis factor alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In human hepatocytes , NOS activity is induced by treatment with a combination of tumor necrosis factor , interleukin 1 , interferon gamma , and LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 3 , IL 6 , interferon gamma , GCSF and to a smaller extent IL 1 beta and GMCSF synergized the differentiation inducing activity of TNF . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although many of these acute phase proteins are predominantly derived from the liver and the response can be elicited from liver cells incubated in tissue culture with cytokines such as interleukin 6 ( IL 6 ) , interleukin 1 ( IL 1 ) , tumor necrosis factor alpha , interferon gamma , leukemia inhibitory factor , interleukin 11 ( IL 11 ) , and oncostatin M , there is now evidence that the response can also be elicited in extrahepatic tissues and cell types . ^^^ In this study , we show that many of the acute phase plasma proteins are expressed in human intestinal epithelial cell lines Caco 2 and T 84 and that their expression is induced or regulated by cytokines IL 6 , IL 1 , interferon , and tumor necrosis factor in a manner characteristic of the acute phase response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
ICAM 1 , but not LFA 3 , was rapidly up regulated by interleukin 1 ( IL 1 ) , tumor necrosis factor alpha ( TNF ) , and to some extent by interferon gamma ( IFN ) and IL 6 , whereas IL 4 had variable low effects , if any . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We examined the effects of Tau Cl on NO production and TNF release by using RAW 264 . 7 cells activated with recombinant interferon gamma ( rIFN gamma ; 50 U / ml ) and lipopolysaccharide ( LPS ; 10 micrograms / ml ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Endothelial leukocyte adhesion molecule 1 and factor 8 expression was not detected on KS derived cells in vitro before or after exposure to interferon gamma or tumor necrosis factor alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The T cell synovial fibroblast interaction also resulted in increased levels of tumor necrosis factor ( TNF ) , interferon gamma , and interleukin 6 in coculture supernatant . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of APO 1 was induced in phytohemagglutinin activated T cells and in a mammary carcinoma cell line by interferon gamma alone and in combination with tumor necrosis factor alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The importance of tumor necrosis factor , interferon gamma , and granulocyte colony stimulating factor in lung infections was first elucidated in animal models and has been confirmed with neutralization studies . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of RPASMC for 4 and 24 h with a combination of tumor necrosis factor alpha , interleukin 1 beta ( IL 1 beta ) , interferon gamma , and LPS resulted in significant LBP mRNA expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Alveolar macrophages from mice treated with rmGM CSF released significantly more tumor necrosis factor alpha ( TNF alpha ) than cells from control mice after in vitro stimulation with lipopolysaccharide ( LPS ) alone ( 2 . 65 + / 0 . 30 vs 1 . 45 + / 0 . 26 ng / ml , p = 0 . 01 ) or with LPS plus murine recombinant interferon gamma ( 4 . 16 + / 0 . 51 vs 2 . 25 + / 0 . 34 ng / ml , p = 0 . 01 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OBJECTIVE : To describe the systemic effects of high dose recombinant tumor necrosis factor alpha ( rTNF alpha ) , recombinant interferon gamma ( rIFN gamma ) , and melphalan administered through hyperthermic isolation perfusion of the limbs ( IPL ) in patients with melanoma and malignant soft tissue tumors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In 55 different keratinocyte strains , there was significant individual variability in ICAM 1 expression by either tumor necrosis factor alpha ( TNF alpha ) or interferon gamma . ^^^ There was no correlation ( r = 0 . 266 , p = 0 . 06 ) in response of the same strain to either TNF alpha or interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vivo transfection of the human interleukin 6 gene into the tumor site reduced methylcholanthrene induced fibrosarcoma growth , and a combination of murine tumor necrosis factor alpha and interferon gamma genes inhibited growth of a renal carcinoma tumor model ( Renca ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Heterogeneity in M phi secretory activity was revealed by ISH analysis of lysozyme , TNF alpha , IL 1 IL 6 and MCP 1 , by in vitro assay of NO and superoxide anion production , and by RT PCR studies of Th 1 ( interferon gamma ) and Th 2 ( IL 4 , IL 13 , IL 10 ) lymphokine mRNA in tissues . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
AS 101 as a new immunomodulator has been shown to induce production of a variety of cytokines such as interleukin 1 , interleukin 2 , colony stimulating factor , interferon gamma and tumor necrosis factor , and demonstrate a potential chemo protection from chemotherapy induced immunosuppression and hematopoietic toxicity in tumor bearing mice and cancer patients on phase 1 and 2 clinical trials . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The release of interleukin 2 , soluble interleukin 2 receptor , interleukin 6 , tumor necrosis factor alpha , interferon alpha 2 , and interferon gamma was assessed by enzyme linked immunosorbent assay . ^^^ RESULTS : For both groups , decreased levels of interleukin 2 and interferon gamma were detected postoperatively , whereas the values for soluble interleukin 2 receptor and tumor necrosis factor alpha showed no significant change . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Heat shock induced cell death in murine microvascular endothelial cells depends on priming with tumor necrosis factor alpha or interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The 4A2 antigen is induced on brain endothelium by interferon gamma ( INF gamma ) but not tumour necrosis factor alpha ( TNF alpha ) , at 6 48 hr . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Glucose induced insulin secretion is inhibited by the cytokines interleukin 1 beta ( IL 1 beta ) , interleukin 6 and tumour necrosis factor alpha ( TNF ) when combined with IL 1 beta in cultured rat islets , by IL 1 beta , TNF and interferon gamma in mouse islets , and by combined treatment of IL 1 beta , TNF and interferon gamma in human islets . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Five examples of these immune modulator proteins encoded by myxoma virus are reviewed : ( 1 ) myxoma growth factor , a member of the epidermal growth factor ligand superfamily ; ( 2 ) SERP 1 , a secreted serine proteinase inhibitor ; ( 3 ) M11L , a receptor like surface protein ; ( 4 ) T 2 , a tumor necrosis factor receptor homologue ; and ( 5 ) T 7 , an interferon gamma receptor homologue . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Concentrations of interleukin 1 ( IL 1 ) , tumour necrosis factor alpha ( TNF alpha ) , and interleukin 6 ( IL 6 ) were measured at 24 hours , and interferon gamma and interleukin 2 ( IL 2 ) were measured at 72 hours , with commercially available assays . ^^^ OUTCOME MEASURES : Production of the monokines IL 1 , TNF alpha , and IL 6 , and of the lymphokines IL 2 and interferon gamma , postoperatively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of acute phase genes is induced by the presence of cytokines in the circulation such as tumor necrosis factor alpha , interferon gamma , and interleukins 1 and 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PURPOSE : Isolated limb perfusion ( ILP ) with tumor necrosis factor ( TNF ) , interferon gamma , and melphalan ( M ) has been reported to result in high response rates for extremity melanoma and sarcoma . ^^^ PATIENTS AND METHODS : Nineteen patients with extremity melanoma ( n = 16 ) or sarcoma ( n = 3 ) , underwent 90 minute ILP with TNF alpha , interferon gamma ( 0 . 2 mg ) , and M ( 10 to 13 mg / L of limb volume ) ( TNF / IFN / M ) ( n = 12 ) , or M alone ( n = 7 ) . ^^^ Cytokine levels and systemic toxicity in patients undergoing isolated limb perfusion with high dose tumor necrosis factor , interferon gamma , and melphalan . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
On concanavalin A challenge , the cytokines tumor necrosis factor alpha ( TNF alpha ) , interleukin 2 , granulocyte macrophage colony stimulating factor , and interferon gamma were detectable in the circulation of the mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have reported previously that SP A enhances concanavalin A induced proliferation , and in this study we examined the secretion of tumor necrosis factor alpha ( TNF alpha ) , interleukins 1 alpha , 1 beta , and 6 , and interferon gamma by human peripheral blood mononuclear cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
At the individual level , mitogen stimulated TNF alpha , interferon gamma , IL 2 , and IL 6 responses were positively correlated , as were mitogen and exoantigen induced TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Plasma levels of tumor necrosis factor alpha , interferon gamma , interleukin 10 ( IL 10 ) , and soluble intercellular adhesion molecule 1 were similarly elevated in the two groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Known poxviral , cytokine response modifiers include CrmA , an inhibitor of the interleukin 1 beta converting enzyme ; several secreted soluble receptors for tumor necrosis factor , interleukin 1 , and interferon gamma ; and poxvirus encoded growth factors resembling epidermal growth factor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of INF gamma on BFU E did not require the presence of interleukin 1 alpha ( IL 1 alpha ) , tumor necrosis factor alpha ( TNF alpha ) , or granulocyte macrophage colony stimulating factor ( GM CSF ) , as its effects were not neutralized by monoclonal antibodies against IL 1 alpha , TNF alpha , or GM CSF . ^^^ INF gamma was not required for IL 1 alpha or TNF alpha induced suppression of BFU E , as their effects were not neutralized by a monoclonal anti INF gamma antibody . ^^^ Suppression did occur , however , if a small concentration of INF gamma that does not inhibit and increasing concentration of TNF alpha were added to culture , suggesting a synergistic effect between INF gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Secreted granulocyte monocyte colony stimulating factor , interferon gamma and tumor necrosis factor were measured in the bioreactor cartridge conditioned medium . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The production of interferon gamma ( INF gamma ) and tumor necrosis factor alpha ( TNF alpha ) from nonadherent LAK cells in LNC was also significantly reduced as compared to that from PBM 4 days after culture , when stimulated with or without tumor target , Raji cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Elaboration of tumor necrosis factor ( TNF ) , interferon gamma ( INF gamma ) , or transforming growth factor beta ( TGF beta ) by activated T cells may directly inhibit hematopoiesis or may interfere with the production of another cytokine necessary for hematopoiesis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After 7 days , the level of tumor necrosis factor alpha ( TNF alpha ) was 220 pg / mL and the interferon gamma ( IF gamma ) production was 150 pg / ml . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This review proposes that host : tumor interactions lead to a nonspecific inflammatory response mediated in part by the chronic production and release of proinflammatory cytokines , including interleukin 1 , tumor necrosis factor alpha , interleukin 6 and interferon gamma , which antagonize the anabolic signals associated with enteral and parenteral nutrition support . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although the effects of exogenous cytokines such as interferon gamma on TNF alpha production by macrophages have been fairly well studied , the intracellular pathways regulating KC TNF alpha synthesis are largely unknown . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Combinations of NO inducers such as IL 1 , lipopolysaccharide , tumor necrosis factor , and interferon gamma also failed to trigger apoptosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , no effects on TNF alpha secretion of MP / MPa monocytes were found by ProTa and / or rIFN gamma , whereas TNF alpha levels from normal monocytes were significantly increased by the two stimuli . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The SN induced cytokine ( interferon gamma , tumor necrosis factor alpha , interleukin 6 ) production in purified NK or T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The enhanced cytotoxicity was ascribed to autocrine effects of secreted TNF on TIL , which included augmentation of adhesion molecule ( CD 2 and CD11a ) and interleukin 2 receptor expression , and elevation of production of interferon gamma , lymphotoxin and granulocyte / macrophage colony stimulating factor and its paracrine effect on target cells to facilitate them to be more susceptible to TIL . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When U 937 cells were cultured with agents that induced the cell differentiation , such as vitamin D 3 , retinoic acid , interferon gamma , and tumor necrosis factor , O 2 ( ) generating activity increased 5 to 200 fold depending on the agent used . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Acute in vitro ethanol treatment down regulated the elevated TNF alpha production by trauma patients ' M 0 after either SEB , muramyl dipeptide ( MDP ) , interferon gamma plus MDP , or lipopolysaccharide ( LPS ) stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma modulated the cytokine production , by increasing the production of TNF alpha , IL 6 and nitric oxide , but IL 1 secretion was only enhanced in C3H / HeJ macrophages stimulated by live ureaplasmas . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our findings suggest that increased concentrations of serum sTNF Rs in HIV infection are linked to immune activation , in which synergistic actions of interferon gamma and the TNF alpha system are likely to play an important role . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In a separate set of studies , cultured intestinal mucosal cells ( Caco 2 ) were exposed to LPS , interleukin 1 ( IL 1 ) , IL 6 , TNF alpha , interferon gamma , prostaglandin E 2 , or dexamethasone . ^^^ Lipopolysaccharide , IL 1 , IL 6 , TNF alpha , gamma interferon , and prostaglandin E 2 did not increase GS activity in Caco 2 cells , whereas dexamethasone increased GS activity and messenger RNA 2 . 5 fold and threefold , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The pp60v src induced cellular resistance to TNF could be abrogated by interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Culture supernatants from blasts cultured with or without TPA showed the production of large amounts of IL 8 , IL 6 , TNF alpha , MIP 1 alpha , IL 10 and interferon gamma and modest amounts of IL 1 alpha , GM CSF and stem cell factor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation with tumour necrosis factor alpha ( TNF alpha ) or interferon gamma ( 100 kU / l ) led to a significant induction of ICAM 1 on the surface of control alveolar macrophages ( 0 . 76 EU + / 0 . 18 , p < 0 . 005 for TNF alpha , 0 . 64 EU + / 0 . 10 , p < 0 . 005 for interferon gamma ) , whereas alveolar macrophages from both patient groups did not respond to cytokines even at high dosages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Some of them ( IL 1 , IL 6 , TNF alpha , interferon gamma ) are produced in increased amounts in vivo , whereas the production of IL 2 is decreased . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Retrovirus infection inhibited release of interleukin 2 ( IL ) and interferon gamma ( IFN ) and some immune functions , whereas it stimulated secretion of IL 4 , IL 5 , IL 6 and tumor necrosis factor alpha ( TNF ) and immunoglobulin ( Ig ) production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor ( TNF ) , interleukin ( IL ) 1 , leukemia inhibiting factor ( LIF ) , interferon alfa , and interferon gamma all decreased adipose tissue LPL activity in intact mice . ^^^ In skeletal and cardiac muscle , only IL 1 and interferon gamma decreased LPL activity , whereas TNF , LIF , and interferon alfa had no effect . ^^^ Tumor necrosis factor ( TNF ) , interleukin ( IL ) 1 , leukemia inhibiting factor ( LIF ) , interferon alfa , and interferon gamma all decreased adipose tissue LPL activity in intact mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) was a less effective inducer of PTX 3 , whereas IL 6 , monocyte chemotactic protein 1 , macrophage colony stimulating factor , granulocyte macrophage colony stimulating factor , and interferon gamma were inactive . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The lesions of BALB / c mice had an increased production of macrophage derived cytokines , such as interleukin ( IL ) 1 beta , IL 6 , tumor necrosis factor , and granulocyte macrophage colony stimulating factor , but not the T cell cytokines IL 3 or interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Since our surfactant preparation stimulated the basal production of tumour necrosis factor alpha ( TNF alpha ) and interleukin 1 beta ( IL 1 beta ) by monocytes , the effect of Survanta , a surfactant extract , on the rIFN gamma induced production of these cytokines by monocytes was studied . ^^^ The results revealed that Survanta caused 80 90 % inhibition of the rIFN gamma induced production of TNF alpha and IL 1 beta by these cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When compared to cytokine generation in TIMC from the chronically inflamed gingivae , more interferon gamma , IL 6 and TNF alpha were generated by OLP TIMC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The growth promoting PCI 50 supernatant was shown to contain 28 + / 0 . 5 pg / ml interleukin 6 ( IL 6 ) in vitro but was negative for interferon gamma , IL 1 , IL 2 , IL 4 , tumor necrosis factor alpha , granulocyte / macrophage colony stimulating factor and IL 12 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Renewed interest has developed in hyperthermic limb perfusion for the treatment of transit and locally advanced recurrent disease because of the availability of melphalan and the recent reports of higher response rates with the addition of tumor necrosis factor and interferon gamma to melphalan . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of lymphokine genes including interleukin 2 ( IL 2 ) , interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , interferon gamma ( IFN g ) , tumor necrosis factor ( TNF ) and lymphotoxin ( LT ) were sequentially monitored in peripheral blood mononuclear cells by Northern blot analysis after stimulation with phytohemagglutinin ( PHA ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This process was not affected by lipopolysaccharide or cytokines such as interferon gamma or tumor necrosis factor alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Of various cytokines tested , tumor necrosis factor alpha ( TNF alpha ) alone counteracted GM 2 and GM 3 induced inhibitions of Ig production and thymidine uptake , whereas other cytokines including IL 1 beta , IL 3 , IL 5 , IL 6 , and interferon gamma each failed to do so . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The in vitro cytotoxicity , phenotype , and cytokine expression pattern as defined by the secretion of tumor necrosis factor alpha ( TNF alpha ) , interferon gamma , granulocyte macrophage colony stimulating factor , IL 4 , IL 6 , and IL 12 were evaluated for each culture . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Growth inhibition of human endothelial cells by human recombinant tumor necrosis factor alpha and interferon gamma . ^^^ We investigated the effect of recombinant tumor necrosis factor alpha ( rTNF alpha ) on the proliferative response of human umbilical vein endothelial cells ( HUVEC ) to normal human serum ( NHS ) , in the absence or the presence of interferon ( IFN ) gamma . rTNF alpha significantly impaired NHS stimulated HUVEC growth at a dose as low as 0 . 1 U / ml . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Combinations of TNF with interferon gamma were highly synergistic both in reducing cell proliferation and in increasing 90K secretion and mRNA expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study measured levels of tumor necrosis factor ( TNF ) alpha , interleukin 2 ( IL 2 ) , and interferon gamma ( IFN G ) in a consecutive group of 31 adult varicella patients presenting within 24 hours of rash onset . ^^^ Tumor necrosis factor , interleukin 2 , and interferon gamma in adult varicella . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It is postulated that the central pathophysiologic mechanism in HIV infection is a high and inefficient production of interferon gamma , genetically determined , leading to a production of autoantibodies that blocks the target organs even the immune system as well as a progressive interleukins levels increase , including tumor necrosis factor alpha ( TNF alpha ) , responsible for many of the symptoms of these patients like fever , headache , fatigue , myalgia , hypotension , seizure and other neurological disorders , hematologic and hepatic disorders . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We observed a significant stimulation of natural killer ( NK ) function and an elevation in the serum level of tumor necrosis factor alpha , interferon gamma , and granulocyte macrophage colony stimulating factor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We also detected cytokines in the skin lesions : ( 1 ) interleukin 1 alpha and tumor necrosis factor alpha were strongly positive in all patients with acute Kawasaki disease , ( 2 ) interleukin 2 and interferon gamma were weakly or partially positive , ( 3 ) no cytokines were detected in the convalescent phase , and ( 4 ) the amounts of cytokines at the site of BCG vaccine inoculations were larger than those at the site of the polymorphous exanthem . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To assess whether RAS oncogenes may affect the expression of cytokines in tumor cells , the presence of interleukins ( IL ) 1 alpha , 1 beta , 4 , 6 , 7 , and 8 , tumor necrosis factor ( TNF ) alpha and interferon gamma mRNA has been analyzed by reverse transcriptase polymerase chain reaction in 19 melanoma clones derived from the metastatic cell line 665 / 2 and previously characterized for RAS mutation and expression . ^^^ With the exception of interferon gamma and IL 4 , all the cytokines tested were expressed by the parental 665 / 2 cell line , whereas IL 1 alpha , IL 6 , and TNF alpha were coordinately transcribed only in the subset of the clones bearing the mutated N RAS gene . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Different cytokines have been identified as mediators involved in the formation and maintenance of these granulomas : IL 2 , interferon gamma , TNF alpha , 1 , 25 ( OH ) 2D3 , IL 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Peritoneal m phi s from LEW / N rats stimulated with LPS / rRaIFN gamma were observed to secrete immunoreactive and bioactive TNF alpha over 18 h in vitro and this cytokine could be dose dependently inhibited by MDL 201 , 112 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Cytokine mRNA expression for interleukin ( IL ) 1 beta , IL 2 , IL 6 , IL 8 , transforming growth factor beta , interferon gamma , and tumor necrosis factor alpha was examined by semiquantitative polymerase chain reaction in 25 human cirrhotic livers and 13 controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In a CAT assay , tumor necrosis factor ( TNF ) alpha and interleukin 1 ( IL 1 ) alpha were shown to repress HPV 16 early gene expression at the transcriptional level through the noncoding region ( NCR ) , whereas IL 6 and interferon gamma did not . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effect of the cytokines interleukin 1 , interleukin 2 , tumor necrosis factor alpha , and interferon alfa on ICAM 1 expression on JHU 022 SCC was also tested . ^^^ Induction was also present after treatment with interleukin 1 and tumor necrosis factor alpha , but not with interleukin 2 or interferon alfa . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These include cell proliferation , the secretion of IL 2 , colony stimulating factor ( CSF ) , interferon gamma , tumor necrosis factor , and interleukin 6 by human MNC , and cell proliferation , IL 2 , IL 3 , and CSF by mouse spleen cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PMNs express only 2500 molecules of beta 1 per cell and this expression is not modulated by agonists such as phorbol myristate acetate , formylmethionyl leucyl phenylalanine , granulocyte macrophage colony stimulating factor , or tumor necrosis factor alpha , which enhance CD11b expression , or by interferon gamma or transforming growth factor beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Analysis of four microsatellites within genes allowed the assignment of the endoplasmic reticulum Ca2+ transport ATPase locus to chromosome 14 , the assignment of the interferon gamma and the diacylglycerol kinase loci to a new linkage group ( 11 ) , and the localization of the tumor necrosis factor beta locus close to the major histocompatibility complex ( SLA ) on chromosome 7 to be confirmed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Effector plus target cell supernatants contained high levels of tumor necrosis factor ( TNF ) alpha and interferon gamma , but these supernatants were not directly toxic to B CLL cells in short term culture . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
All orchitogenic T cell lines and clones expressed CD 4 and the alpha beta T cell receptor ; and when activated , they produced interleukin 2 , interferon gamma , and tumor necrosis factor ( TNF ) , but not interleukin 4 . ^^^ Importantly , since disease transfer was significantly and reproducibly attenuated when recipients were injected with neutralizing antibody to TNF , but not neutralizing antibody to interferon gamma , TNF has been defined as a cytokine important in the pathogenesis of this autoimmune disease . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have cloned from murine macrophages a cDNA coding for a new protein of the scavenger receptor family whose mRNA is increased very strongly by adherence and moderately by exposure to tumor necrosis factor and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Of the various retinoids studied , all trans retinoic acid ( RA ) was most potent ; it almost completely inhibited the production of TNF by macrophages activated with endotoxin and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The use of human immunoregulatory recombinant cytokines ( interleukin 1 beta , interferon gamma , tumor necrosis factor alpha ) failed to augment in vitro suppressed neutrophil antibacterial ( phagocytic ) activity in patients with COB . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In long term exposure studies , IMCD cells were incubated with tumor necrosis factor ( TNF ) and interferon gamma ( IFN ) up to 72 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
B6MP102 cells incubated with interferon gamma and SEB secreted TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
From these and our earlier data it is evident that prenatal exposure to low doses of benzodiazepines can result in long lasting alterations of the cytokine network , as indicated by reduced release of TNF alpha , IL 1 , IL 6 , IL 2 and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We also show that the regulatory effect of the CTLs is initially mediated by interferon gamma and tumor necrosis factor alpha , is delayed in onset , and becomes independent of these cytokines shortly after it begins . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro studies indicate that C1INH mRNA and protein levels are increased by up to 20 fold after stimulation with interferon gamma ( gamma IFN ) and to a lesser extent in response to alpha interferon ( alpha IFN ) , tumor necrosis factor alpha ( TNF alpha ) , Interleukin 6 ( IL 6 ) and monocyte colony stimulating factor ( M CSF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This inhibitory effect resulting from TNF neutralization was not observed using pooled hamster immunoglobulin G , or hamster monoclonals against murine interleukin 1 alpha ( IL 1 alpha ) , IL 1 beta , or interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Symptomatic therapy has allowed patients to tolerate somewhat higher doses , but has not addressed the underlying mechanisms of these toxicities which may involve mediators such as tumor necrosis factor , interferon gamma , and nitric oxide . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Incubation of endothelial cells ( EC ) with interleukin 1 alpha ( IL 1 alpha ) , in contrast to incubation with interferon gamma or tumor necrosis factor alpha , resulted in an enhanced IgG AEA binding . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We argue that IL 1 potentiated by other cytokines ( tumor necrosis factor alpha , interferon gamma ) is an important effector molecule involved in both early and late events in the immune mediated process that leads to beta cell destruction and IDDM . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , CCL 185 does not show detectable production of IL 1 , tumor necrosis factor alpha or interferon gamma after incubation with rhIL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Administration of roxithromycin for 28 days resulted in increased synthesis of interleukin ( IL ) 1 and tumour necrosis factor ( TNF alpha ) production by macrophages , and the production of IL 2 , IL 4 and interferon gamma by splenocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
All these syntheses were up regulated by interferon gamma and tumor necrosis factor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Messenger RNAs for interleukin 2 ( IL 2 ) , interferon gamma , and tumor necrosis factors ( TNF ) alpha and beta were not expressed at detectable levels in spleens of unstimulated animals but became visible already 30 min after intraperitoneal application of 50 micrograms staphylococcal enterotoxin B . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found that none of the cytokines interleukin 1 , interleukin 2 , interferon gamma , tumor necrosis factor alpha , lymphotoxin , or BCG alone were cytotoxic against the bladder carcinoma cell lines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These changes seem to be dependent on factors , such as TNF alpha , interleukin 1 and interferon gamma , which are released in inflammatory processes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma treatment decreased receptor activity in both human and rat macrophages , but increased TNF alpha binding to U 937 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We previously reported that interleukin 4 ( IL 4 ) and either tumor necrosis factor alpha ( TNF ) or interferon gamma ( IFN ) synergistically inhibit melanoma cell growth and induce cell differentiation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using this sensitive technique it was not possible to detect mRNA for IL 1 alpha , IL 2 , IL 4 , IL 5 , IL 7 , TNF alpha , or interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have previously shown that continuous administration of anti interleukin 10 ( anti IL 10 ) antibodies ( Abs ) to BALB / c mice modifies endogenous levels of autoantibodies , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma , three immune mediators known to affect the development of autoimmunity in `` lupus prone ' ' New Zealand black / white ( NZB / W ) F 1 mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A limb saving complete remission of a locally advanced liposarcoma of the left thigh was achieved with isolated regional perfusion of the limb with tumor necrosis factor alpha , interferon gamma and melphalan . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Hepatocytes of such rats responded to in vitro stimulation for 20 hr by single cytokines , tumor necrosis factor , interleukin 1 beta and interferon gamma with enhanced nitric oxide formation . ^^^ In combination , interferon gamma and endotoxin had greater synergistic effect on hepatocytes than did tumor necrosis factor and endotoxin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Soluble factors such as Interleukin 2 and Interferon gamma released by T cells and Interleukin 1 , Interleukin 6 and TNF released by monocytes have been shown to play key roles in proliferation , activation and differentiation of immune cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In a double blind placebo controlled cross over study , healthy volunteers were given 2 IU r hGH as an intravenous infusion . r hGH did not influence the subpopulations of blood mononuclear cells ( BMNC ) , natural killer cell activity , in vitro proliferative responses or production of interleukin 1 alpha ( IL 1 alpha ) , IL 1 beta , IL 2 , IL 6 , IL 8 , tumor necrosis factor alpha ( TNF alpha ) , TNF beta or interferon gamma in supernatants from BMNC stimulated with either lipopolysaccharide or phytohemagglutinin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The number of peritoneal M phi and the production of cytokines ( interleukin 1 beta , interferon gamma and tumor necrosis factor ) was increased by the combined treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Microglial cells incubated with recombinant TNF alpha in combination with a non activating concentration of rIFN gamma released substantial amount of RNIs . ^^^ Neutralizing antibodies against mouse TNF alpha inhibited the release of RNI by rIFN gamma activated macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The response of the host involves production of a large number of endogenous mediators including a number of cytokines that are currently the focus of many studies : tumor necrosis factor ( TNF alpha ) , interleukins ( IL 1 and IL 6 ) , and interferon gamma ( IGN gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Effect of interferon alpha , interferon gamma and tumor necrosis factor on the procoagulant activity of human cancer cells . ^^^ It is not known whether the different cytokines may influence the procoagulant activity of cancer cells ; the purpose of this study was to investigate the effect of interferon alpha , interferon gamma and tumor necrosis factor on the procoagulation capacity of human cancer cells cultured `` in vitro ' ' or isolated from tumor tissues . ^^^ Furthermore , short term incubation of cells isolated from tumor tissues with interferon gamma or tumor necrosis factor resulted in a significant increase of procoagulant activity , while interferon alpha had no effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Based on our previous finding of elevated interferon gamma levels in the cerebrospinal fluid ( CSF ) , but not in serum , of HTLV 1 associated myelopathy ( HAM ) , we assayed serum and CSF levels of granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 1 ( IL 1 ) and tumor necrosis factor alpha ( TNF alpha ) in 20 HAM . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study investigated IL 1ra and IL 1 alpha accumulation by cultured keratinocytes stimulated by tumor necrosis factor alpha ( TNF alpha ) , IL 3 , IL 4 , IL 6 , IL 10 , interferon gamma , granulocyte macrophage colony stimulating factor , granulocyte colony stimulating factor , and macrophage colony stimulating factor and by various extracellular matrix proteins , conditions that these cells may encounter in normal or inflamed skin in vivo . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Peak levels of tumor necrosis factor and interferon gamma were not affected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma and TNF alpha were utilized as the `` physiological ' ' inducers of differentiation associated markers . ^^^ In contrast to these data , interferon gamma , TNF alpha , retinoic acid and 1 , 25 ( OH ) 2 vitamin D 3 induced the up regulation of leukosialin in a promyelocytic leukemia cell line HL 60 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RCEC were stimulated in a dose time dependent manner with : IL 1 beta ( 0 . 01 to 1 . 0 ng / ml ) , TNF ( 0 . 1 to 10 ng / ml ) , LPS ( 0 . 1 to 10 micrograms / ml ) or INF gamma ( 10 1000 U / ml ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To establish whether EA hy 926 could be used as a model for endothelial cells ( EC ) in leucocyte EC adhesion interactions , the effect of interleukin 4 ( IL 4 ) , tumour necrosis factor ( TNF ) or interferon gamma ( IFN ) stimulation on their adhesiveness and expression of E selectin , vascular cell adhesion molecule 1 ( VCAM 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) was compared with that of HUVEC and A 549 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
No detectable fluctuations were observed in circulating levels of tumor necrosis factor , interferon gamma , or interleukin 6 during the course of this study . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The assay is 100 1000 fold less sensitive to other inhibitory molecules such as interferon beta , interferon gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There was no accumulation of metallothionein with interleukin 1 beta , tumor necrosis factor alpha or interferon gamma ( 1 10 10 ( 5 ) U / L ) alone , but interleukin 1 beta and tumor necrosis factor alpha enhanced the response obtained with Zn+dexamethasone with and without interleukin 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To further investigate their regulation the human leukemic cell line HL 60 which can be induced to differentiate to monocytes / macrophages by 12 O tetradecanoylphorbol 13 acetate ( TPA ) , 1 , 25 ( OH ) 2 Vitamin D 3 ( VD 3 ) , tumor necrosis factor alpha ( TNF alpha ) and interferon g ( IFN g ) were analyzed for expression of MRP8 / MRP14 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment with PTX and either interferon gamma , interferon alpha / beta , tumor necrosis factor , or lymphotoxin increased the levels of K and D expression above those observed following treatment with either PTX or cytokines alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Normal host ( NH ) and TBH splenic Ia ( + ) depleted ( Ia ) m phi s synthesized more TNF alpha than their respective whole populations ( WPs ) when cultured with lipopolysaccharide and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These `` short term ' ' DETC lines also expressed mRNA for IL 2 , interferon gamma , IL 1 alpha , IL 3 , IL 6 , IL 7 , tumor necrosis factor alpha and beta , and granulocyte macrophage colony stimulating factor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Culture supernatants were tested by ELISA for the presence of granulocyte / macrophage colony stimulating factor , interferon gamma , and tumor necrosis factor alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
HT 115 cells were strongly scattered by the conditioned media but not by interleukins IL 1 , 2 , 4 , 6 , 8 , 10 , TNF alpha , TGF beta , EGF , GM CSF , IGF 1 , PDGF , interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PATIENTS AND METHODS : We measured serum levels of tumor necrosis factor ( TNF ) , interleukin ( IL ) 1 , IL 2 , IL 6 , and interferon gamma ( IFG ) serially for the first 48 h following the onset of hypotension ( systolic blood pressure < 90 mm Hg ) thought likely to be due to sepsis in all patients presenting to one ICU . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The results reported here indicate that a wide range of cytokines , including interleukin ( IL ) 4 , IL 10 , interferon gamma , tumor necrosis factor beta and macrophage inflammatory protein 1 alpha , are indeed differentially expressed in acute and chronic GVHD and support the concept that the pathology peculiar to acute or chronic GVHD may arise due to differential cytokine expression by activated T cells . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , IL 2 promotes release of interferon gamma as well as tumor necrosis factor and other proinflammatory cytokines . ^^^ Therapeutic options to `` downregulate ' ' this cascade have gradually evolved from global nonspecific immunosuppressive techniques ( total body irradiation , antilymphocyte serum ) to increasingly specific modalities currently being studied , including monoclonal antibodies against the IL 2 receptor ( thus targeting only vigorously proliferating T cells ) , antibodies against specific cytokines ( interferon gamma , tumor necrosis factor ) , and now `` designer ' ' antibody toxin conjugate molecules that deliver toxins to selected receptor targets . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
While acute ethanol treatment did not induce TNF alpha in M phi , it was a potent down regulator of M phi TNF alpha production whether induced by the combination of interferon gamma plus muramyl dipeptide ( MDP ) ( P < 0 . 001 ) , lipopolysaccharide ( LPS ) alone ( P < 0 . 01 ) , or interferon gamma plus LPS . ^^^ We have previously shown that the majority of posttrauma elevated M phi TNF alpha is produced by the M phi subpopulation expressing high affinity type 1 Fc gamma receptors ( Fc gamma RI ) . ^^^ When the Fc gamma RI cross linking stimulated M phi subpopulation was treated with acute ethanol , TNF alpha production was suppressed again both in in vivo preactivated M phi of trauma patients and in M phi of normal controls . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokines interleukin 1 ( IL 1 ) and tumor necrosis factor ( TNF ) , as well as interferon gamma and interleukin 8 , are discussed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The allelic products were visualized in untreated and interferon gamma + tumor necrosis factor alpha treated aliquots of tumor cells and in the patient ' s blood lymphocytes by the one dimensional isoelectric focusing method . ^^^ In vitro exposure to interferon gamma and tumor necrosis factor alpha elevated the level of MHC class 1 expression in 24 of 52 tumors and corrected the assembly defect seen in 2 cases . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Several lymphokines ( e . g . , interferon gamma , tumor necrosis factor , interleukin 4 and interleukin 10 ) are identified among the visualized set of secreted polypeptides . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , this cell line produces high levels of interferon gamma and tumor necrosis factor alpha on stimulation with anti CD 3 and / or anti CD 2 MoAb both in the presence or absence of IL 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma completely inhibits the IL 6 mediated myeloma cell proliferation without affecting the endogenous IL 6 production and IFN alpha and TNF alpha stimulate the proliferation of our IL 6 dependent myeloma cell lines by inducing an autocrine production of IL 6 in these cell lines . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This clone produced interferon gamma , tumor necrosis factor and interleukin 2 , but not interleukin 4 , when co cultured with PPD and irradiated syngeneic C57BL / 6 spleen cells , suggesting that this clone could thus be assigned to the Th 1 subset . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Though the relationships among the components of the immune response are complex , it seems likely that in response to mycobacterial infection associated with active disease , cytokines such as TNF alpha and IL 1 beta are produced ; these cytokines serve to recruit more lymphocytes , generally of the T ( H ) ( T helper ) phenotype , which then produces substances such as the macrophage activating factor interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lymphocytes extracted from the tumour are transfected with genes capable of increasing their cytotoxic action in situ when reinjected ( TNF , interleukin 2 , interferon gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This increase of sPLA 2 was presumably not due to a direct effect of IL 2 on , for example , hepatocytes , since this cytokine , in contrast to IL 1 , IL 6 , TNF and interferon gamma , was not able to induce the synthesis of sPLA 2 by Hep G 2 cells in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have recently described the induction of IP 10 mRNA in mouse mesangial cells stimulated with lipopolysacharide , interferon gamma , and tumor necrosis factor alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tick countermeasures to host defenses reduce T lymphocyte proliferation , elaboration of the TH 1 cytokines interleukin 2 and interferon gamma , production of macrophage cytokines interleukin 1 and tumor necrosis factor , and antibody responses . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Pretreatment with IL 10 markedly reduced ( P < . 05 ) serum levels of tumor necrosis factor alpha ( 79 % ) , IL 6 ( 94 % ) , and IL 1 alpha ( 69 % ) , but it significantly inhibited splenocyte proliferation ( 32 % ) and IL 2 ( 40 % ) , IL 6 ( 49 % ) , and interferon gamma ( 54 % ) release of splenocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The aim of this study was to define the effects of tumor necrosis factor alpha ( TNF ) and interferon gamma ( IFN ) on nitric oxide production , insulin secretion , and DNA damage in islets from unweaned rats . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have examined the effect of interferon ( IFN ) gamma and tumor necrosis factor ( TNF ) alpha on the development of the disease . ^^^ Orally administered interferon gamma but not tumor necrosis factor alpha suppress infection with Salmonella typhimurium in a mouse model . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Such cytokines as interferon gamma , IL 3 or granulocyte macrophage colony stimulating factor amplify the inflammatory response by increasing the production of IL 1 and TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Regression of human breast cancer xenografts in response to intralesional treatment with interferons alpha and gamma potentiated by tumor necrosis factor . ^^^ The potentiating effects of human recombinant tumor necrosis factor alpha ( rTNF alpha ) on the antitumor actions of recombinant interferon gamma ( rIFN gamma ) and of natural interferons alpha and gamma combined ( nIFN alpha / nIFN gamma ) were studied on human breast cancer xenografts growing bilaterally in nude mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Those findings indicate that interferon gamma or TNF alpha produced by infiltrating inflammatory cells may induce expression of these immunologic markers or adhesion molecules . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum neopterin , soluble IL 2 receptors , beta 2 microglobulin , TNF alpha and interferon gamma were determined by Elisa . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this investigation we studied the regulation of NOS 2 in a human liver epithelial cell line ( AKN 1 ) which expresses high levels of NOS 2 mRNA and protein in response to tumor necrosis factor alpha , interleukin 1 beta , and interferon gamma ( cytokine mix , CM ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the ten patients studied , neither interferon alpha alone or together with either interferon gamma , IL 1 , IL 4 , IL 6 , TNF , or serum containing high levels of soluble CD 23 was able to induce proliferation of B CLL cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Levels of IL 6 , tumor necrosis factor alpha and interferon gamma were not significantly elevated in patients with BD . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine transcription of beta actin , interleukins ( IL ) IL 1 alpha , IL 1 beta , IL 2 , IL 4 , IL 6 , IL 10 , tumor necrosis factor ( TNF ) alpha , TNF beta and interferon gamma was detected from concanavalin A activated peripheral blood mononuclear cells and CD4+ purified T lymphocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Exposure of GO OEC and normal OEC monolayers to interferon gamma , interleukin 1 alpha and tumor necrosis factor alpha resulted in marked up regulation of immunoreactivity for ICAM 1 and in induction of ELAM 1 and VCAM 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The emphasis of this presentation is on the role of tumor necrosis factor , interleukins and interferon gamma in endotoxic shock and acute phase response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , production of neopterin , a presumed primate homologue of nitric oxide in lower animals , was increased in THP 1 cells stimulated with INF gamma plus TNF alpha and alpha MSH significantly inhibited this production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study cultured human peripheral blood mononuclear cells were polyclonally stimulated and then analyzed for interleukin 1alpha ( IL 1alpha ) , IL 1beta , IL 1ra , IL 2 , IL 4 , IL 6 , IL 8 , IL 10 , tumor necrosis factor alpha ( TNF alpha ) , TNF beta , interferon gamma , granulocyte colony stimulating factor and granulocyte macrophage colony stimulating factor production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NO production during 10 infection was critically dependent on the cytokines tumor necrosis factor and interferon gamma , and on the presence of both CD4+ and CD8+ T lymphocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These pure CD 3 positive PBL ( CD3+PBL ) and TIL ( CD3+TIL ) were cultured with pokeweed mitogen and the levels of the cytokines interleukin 1alpha ( IL 1 alpha ) , IL 1 beta , IL 2 , interferon gamma ( IFNgamma ) , and tumor necrosis factor alpha ( TNFalpha ) measured in the 4 day post inductional cell culture supernatants . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In primary cultures , interferon gamma ( IFN ) , interleukin 1 beta ( IL 1 ) , or tumor necrosis factor alpha ( TNF ) separately did not stimulate nitrite production , whereas IFN combined with IL 1 or TNF synergistically induced iNOS , both at the level of steady state mRNA and nitrite accumulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Positive correlations were observed in multiple sclerosis between TNF alpha and LT mRNA expressing blood mononuclear cells , MBP reactive TNF alpha and LT mRNA expressing cells , and TNF alpha and interferon gamma ( INF gamma ) mRNA expressing mononuclear cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNFalpha ) and interferon gamma ( IFNgamma ) are important immunomodulators . ^^^ Peripheral natural killer cell activity and intraperitoneal soluble p 55 tumor necrosis factor receptor in patients with malignant ascites : two possible indicators for response to intraperitoneal combined tumor necrosis factor alpha and interferon gamma treatment . ^^^ Tumor necrosis factor alpha ( TNFalpha ) and interferon gamma ( IFNgamma ) are important immunomodulators . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PURPOSE : To evaluate response rates and systemic and regional toxicity of hyperthermic isolated limb perfusion ( ILP ) for treatment of in transit metastases of extremity melanoma using escalating dose tumor necrosis factor ( TNF ) in conjunction with melphalan and interferon gamma ( IFN ) . ^^^ Treatment of patients with melanoma of the extremity using hyperthermic isolated limb perfusion with melphalan , tumor necrosis factor , and interferon gamma : results of a tumor necrosis factor dose escalation study . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MCP expression was up regulated by IL 1 beta , but not by TNF alpha or INF gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment with tumor necrosis factor alpha ( TNFalpha ) or interferon gamma ( IFNgamma ) induced lithostathine / reg mRNA expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A phase 1 trial was initiated to define the feasibility and safety of single lung isolation perfusion with tumor necrosis factor alpha , interferon gamma , and moderate hyperthermia for patients with unresectable pulmonary metastases . ^^^ Twenty patients with lung metastases ( Ewing ' s , 2 ; sarcoma , 8 ; melanoma , 6 ; other , 4 ) were considered for single lung isolation perfusion with 0 . 3 to 6 . 0 mg of tumor necrosis factor alpha and 0 . 2 mg interferon gamma delivered through an oxygenated pump circuit . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Limb salvage can be achieved by isolated limb perfusion ( ILP ) with tumor necrosis factor alpha ( TNF alpha ) , interferon gamma and melphalan . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Levels of interferon gamma , granulocyte macrophage colony stimulating factor ( GM CSF ) , tumour necrosis factor alpha ( TNF alpha ) and IL 10 produced by stimulated CD 3 CD8 dGL clones were greater than those produced by stimulated CD 3 CD8+ dGL clones . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cathepsin D protease mediates programmed cell death induced by interferon gamma , Fas / APO 1 and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The present study shows that , during macrophage activation , protein tyrosine phosphorylation of STAT 1 alpha and ERK 2 occurred as an immediate early signal , whereas maximum TNF alpha mRNA transcription appeared at 3 hr , precursor TNF alpha formation at 3 to 4 hr , and TNF alpha release at 5 to 6 hr after stimulation of an RPMI 1640 based induction medium containing lipopolysaccharide ( 100 ng / ml ) , interferon gamma ( 100 U / ml ) , and 0 . 5 % bovine serum albumin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The anergic T cells failed to produce IL 2 , IL 5 , IL 10 , interferon gamma , tumor necrosis factor alpha , and granulocyte / macrophage colony stimulating factor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The role of free radicals as well as cytokines ( IL 1 , tumor necrosis factor alpha , interferon gamma ) and nitric oxide in the immune mediated processes leading to the beta cell destruction during IDDM is described . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
High plasma tumor necrosis factor ( TNF ) alpha concentrations and a sepsis like syndrome in patients undergoing hyperthermic isolated limb perfusion with recombinant TNF alpha , interferon gamma , and melphalan . ^^^ OBJECTIVES : To describe the postoperative course of patients who underwent hyperthermic isolated limb perfusion with recombinant tumor necrosis factor ( TNF ) alpha and melphalan after pretreatment with recombinant interferon gamma as treatment for recurrent melanoma , primary nonresectable soft tissue tumors , planocellular carcinoma , or metastatic carcinoma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In patients with in transit melanoma or unresectable sarcoma , treatment with hyperthermic isolated limb perfusion using TNF , interferon gamma , and melphalan has resulted in response rates exceeding 90 % . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha , interferon gamma etc . ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In skin lesions of lepromatous leprosy patients TNF alpha , interleukin 1 beta ( IL 1 beta ) , and INF gamma production are found to be rare , whereas these cytokines are well expressed in skin lesions of patients with tuberculoid leprosy . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Rat islets cultured for 4 days in the presence of 5 , 10 , and 30 mmol / l glucose were exposed to interferon gamma ( IFN , 500 U / ml ) and tumor necrosis factor alpha ( TNF , 250 U / ml ) for the last 24 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Plasma levels of circulating ICAM 1 ( cICAM 1 ) were significantly higher in 246 children with acute malaria than in 156 children with non malarial illnesses . cICAM 1 levels correlated with levels of tumour necrosis factor ( TNF ) , interleukin 1 alpha ( IL 1 alpha ) and interferon gamma , supporting the view that these cytokines are responsible for a general upregulation of ICAM 1 expression in malaria . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CD4+ and CD8+ T cell clones were stimulated by bacterial superantigens in the presence or absence of monoclonal antibodies ( mAbs ) against the KIR NKB 1 or MHC class 1 molecules , and production of tumor necrosis factor alpha and interferon gamma was evaluated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The methylxanthines attenuated systemic release of endogenous tumor necrosis factor ( TNF ) and interferon gamma during endotoxic shock , and potently up regulated early production of circulating interleukin 10 and interleukin 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We show here that neutrophils within human buffy coat preparations stimulated with a mixture of interleukin 1 , tumor necrosis factor alpha , and interferon gamma contain inducible NO synthase mRNA and protein , one of the enzymes responsible for NO production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cells were cultured to confluence and incubated with interferon gamma , tumor necrosis factor , and lipopolysaccharide in the combined presence of the antioxidants ascorbic acid , Trolox , catalase , or superoxide dismutase , singly and in combination , for 48 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here , using consensus cytokine nucleic acid sequences , we adapt a previously described reverse transcription quantitative competitive polymerase chain reaction technique to measure interleukin 2 ( IL 2 ) , IL 4 , IL 6 , IL 10 , IL 12 , interferon , gamma tumor necrosis factor alpha , and glyceraldehyde 3 phosphate dehydrogenase mRNA expression in the cow , cat , dog , horse , and pig . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In situ hybridization was used to identify the mRNA for specific proteins : interleukin ( IL ) 1 alpha and beta , IL 2 , IL 6 , transforming growth factor beta , tumor necrosis factor alpha ( TNF alpha ) , platelet derived growth factor alpha ( PDGF alpha ) , and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We attempted to evaluate the spatial and temporal distribution of five principal cytokines ( interleukin 1 beta [ IL 1 beta ] , platelet derived growth factor AA [ PDGF AA ] , basic fibroblast growth factor [ bFGF ] , interferon gamma [ INF gamma ] , and tumor necrosis factor alpha [ TNF alpha ] ) during the development of IH in a rat vein graft model . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , we detected tumor necrosis factor , interferon gamma , interleukin ( IL ) 1 beta , and IL 6 in the DRGs from AIDS patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1 , TNF , UV light and osmotic stress , but not PMA , LPS , IL 3 , IL 4 , IL 6 , IL 8 , IL 10 , IL 13 , GM CSF , PDGF , bFGF , TGF beta and interferon gamma were able to stimulate jun kinase activity in HMC , suggesting that jun kinase is selectively mediating signal transduction of the proinflammatory cytokines IL 1 and TNF as well as of cellular stress in HMC . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The activation of intraepithelial lymphocytes showed the gene transcription of interferon gamma and tumor necrosis factor alpha , as measured by means of the reverse transcriptase polymerase chain reaction , and this activation was antagonized by 5 aminosalicylic acid . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Earlier and stronger interleukin ( IL ) 12 , interferon gamma ( IFN ) gamma , and tumor necrosis factor responses were found in Borrelia burgdorferi infected , vitamin A deficient mice compared with controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Stimulation of synovial fibroblasts by the proinflammatory cytokines tumor necrosis factor alpha , interleukin 1beta , and interferon gamma resulted in a dose dependent increase in lymphocyte adhesion to synovial fibroblasts . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These T cell lines may exhibit either tumor specific cytotoxicity against autologous tumor cells , or produce cytokines ( interferon gamma , tumor necrosis factor , and granulocyte stimulating factor ) either in antigen dependent ( tumor specific ) or an antigen independent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In cytokines , the concentration of tumour necrosis factor alpha ; ( TNF alpha ; ) in CSF increased transiently at 4 hours after LPS injection , while interleukin 1 beta , interleukin 6 , interferon gamma , did not change . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We tested the effect of these porphyrins on lymphocyte proliferation and secretion of interleukin 2 , interleukin 3 , tumor necrosis factor alpha ( TNFalpha ) and interferon gamma ( IFNgamma ) , by human or murine mononuclear cells ( MNC ) without an activating light . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma and tumor necrosis factor inductive activities were studied by using isolated mouse spleen lymphocytes and peritoneal macrophages , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , the expression of ICAM 1 , VCAM 1 , E selectin as well as PECAM 1 on cultured HOMES was studied over 5 , 24 , 48 and 72 h exposure to IL 1 beta , interferon gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma , IL 10 , and tumor necrosis factor could be induced throughout the treatment period by IL 12 , indicating that repeated injections of IL 12 do not induce a state of tachyphylaxis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , PDGF AA , PDGF BB , bFGF , interleukin 6 , interferon gamma and tumor necrosis factor alpha all stimulated smooth muscle cell proliferation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tricyclic antidepressants inhibit IL 6 , IL 1 beta and TNF alpha release in human blood monocytes and IL 2 and interferon gamma in T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These cells accumulated TNF mRNA and secreted TNF protein in a dose dependent fashion after exposure to either LPS or recombinant murine interferon gamma ( riFN gamma ) . ^^^ Pre exposure of RAW 264 . 7 cells to relatively high concentrations of LPS ( > 10 ng / mL ) resulted in diminished production of TNF in response to subsequent challenge with either LPS or riFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Rat pleural mesothelial cells were exposed to various combinations of tumor necrosis factor , interleukin 1 , interferon gamma , and lipopolysaccharide or to the nitric oxide donors : 6 morpholino sydnonimine , S nitroso N acetyl D , L penicillamine , sodium nitroprusside , and spermine NO adduct for 24 48 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 6 . interferon 7 ( IFN 7 ) and tumour necrosis facor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Recently there has been renewed interest in the use of ILP based upon the very promising results using a combination of tumor necrosis factor , melphalan , and interferon gamma which have produced complete response ( CR ) rates of almost 90 % . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We show here that highly purified MHC class II+ microglia when tested directly ex vivo do indeed support an effector response by an encephalitogenic myelin basic protein reactive CD 4 T cell line from which production of the proinflammatory cytokines , interferon gamma and tumor necrosis factor , is elicited , but not interleukin ( IL ) 2 secretion or proliferation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Maximal plasma concentrations of nitrate plus nitrite were correlated to the degree of hypotension ( r = 0 . 64 , p = 0 . 02 ) and levels of tumor necrosis factor ( TNF ) alpha ( r = 0 . 51 , p = 0 . 05 ) and soluble TNF receptors p 55 and p 75 ( r = 0 . 58 , p = 0 . 03 and r = 0 . 54 , p = 0 . 04 , respectively ) but not to levels of interferon gamma or interleukin 10 ( p > 0 . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Temporal responses of interleukin 1 alpha , tumor necrosis factor alpha , interleukin 2 , and interferon gamma evoked by inhaled SEB were enhanced by LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Peripheral blood monocytes proved abnormally permissive to the intracellular growth of Mycobacterium avium and M . kansasii , and expressed normal number of receptors to interferon gamma , but reduced numbers of receptors to granulocyte monocyte colony stimulating factor and tumor necrosis factor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Two cytokines , interferon gamma and tumor necrosis factor alpha , which can inhibit cell proliferation or induce cell death , have been found to inhibit transplasma membrane electron transport . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Activation of macrophages by interferon gamma could not recover their TNF productivity in the presence of P . falciparum infected erythrocytes , but it enhanced their TNF productivity in the presence of lipopolysaccharide ( s ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( 0 . 4 400 ng / ml ) induced a dose related increase in IL 6 secretion and showed a synergistic effect on that secretion in combination with TNF alpha ( 10 ng / ml ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the culture condition of IL 12 and a low dose of IL 2 , a synergistic effect was observed in the production of such cytokines as interferon gamma , tumor necrosis factor alpha ( TNF alpha ) , and TNF beta , as well as in tumor cytotoxicity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine mRNA levels for interleukin ( IL ) 1 beta , IL 2 , IL 4 , IL 8 , IL 10 , tumor necrosis factor alpha , and interferon gamma , as well as Granzyme A and IL 2R p 55 and p 75 transcripts were determined and compared in peripheral blood mononuclear cells and in separated T cell subsets . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CONCLUSIONS : Four children with severe mycobacterial infections had a mutation in the gene for interferon gamma receptor 1 that leads to the absence of receptors on cell surfaces and a functional defect in the up regulation of tumor necrosis factor alpha by macrophages in response to interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Malnourished mice exhibited a tissue specific diminution in the expression of interferon gamma , tumor necrosis factor alpha , and the inducible form of nitric oxide synthase in the lungs , but not the liver . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Steroid hormones and cytokines ( interleukin 1 alpha , beta , tumor necrosis factor , interferon gamma ) have a major regulatory influence on protein secretion , including the secretion of immunoglobulin into the saliva . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
At 4 weeks , interleukins 2 , 3 , 5 , 6 , and 10 ; interferon gamma ; tumor necrosis factor alpha ; and granulocyte macrophage colony stimulating factor were detected in gastritic mucosa , but interleukin 4 was not . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lymphocyte products that are central to the activation of macrophages to increased mycobacterial killing include tumor necrosis factor alpha ( TNF alpha ) , interferon gamma , and granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
If added to cells activated by interferon gamma and lipopolysaccharide , radicicol analogue A not only inhibited the secretion of IL 1 beta but also induced an extremely rapid degradation of IL 1 beta , IL 6 and TNF alpha mRNA to undetectable levels within 5 8 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To better understand the immunopathology of brain injury , we studied the role of inflammatory cytokines such as tumor necrosis factor alpha , interleukin ( IL ) 6 , IL 2 , interferon gamma and IL 1 beta in the production of arachidonic acid metabolites in cells from fetal human brain . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin ( IL ) 1 beta , IL 6 , interferon gamma , and tumor necrosis factor were also measured . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Finally , endogenous NO formation , induced in hepatocytes stimulated with interferon gamma and interleukin 1beta , led to the formation of GSNO and GSSG , induced HSP 70 , and attenuated TNFalpha mediated cytotoxicity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both motapizone as well as rolipram dose dependently ( 0 . 1 10 mg / kg ) attenuated the systemic release of TNF and interferon gamma as initiated by injection of Con A ( 25 mg / kg ) or SEB ( 2 mg / kg ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Intact and ADX rats were treated with HU , mRNA was extracted from spleen cells 2 and 24 hr after treatment and message levels for IL 1 alpha , IL 2 , IL 4 , IL 6 , TNF alpha and interferon gamma were evaluated using the reverse transcriptase polymerase chain reaction technique . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Incubation with inflammatory cytokines ( interferon gamma , interleukin 1 , and tumor necrosis factor alpha ) or phorbol ester failed to induce or up regulate C5aR expression on fetal or adult brain cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Resistance to CM in TNF alpha / beta deficient mice is associated with reduced interferon gamma and interleukin 12 expression in the brain , in the absence of increased T helper type 2 cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Hepatocytes exposed to the cytokines tumor necrosis factor alpha ( TNFalpha ) and interferon gamma ( IFNgamma ) , in combination with a nontoxic dose of lead ( Pb ) , exhibited significantly greater cytotoxicity compared to cytokine treated hepatocytes after 24 hr treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Adhesion is increased when fibroblast monolayers are exposed to tumor necrosis factor alpha ( TNF ) alone and in combination with interferon gamma ( IFN ) or interleukin 4 ( IL 4 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A1 cells , which were activated by MTP Chol NC and interferon gamma , TNF alpha production seemed to act as a second messenger . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment with a neutralizing anti TNF monoclonal antibody 2 h before inoculation strongly reduced lung TNF activity , but only modestly diminished lung interleukin ( IL ) 1beta levels , and did not significantly influence lung IL 6 , IL 10 , and interferon gamma concentrations . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There was no cytokine switch by our Th 1 cells after exposure to their specific antigen , but increased secretion of interferon gamma and tumor necrosis factor alpha was demonstrated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have previously observed a significant elevation in interleukin 1 ( IL 1 ) and tumour necrosis factor alpha ( TNF alpha ) secretion in mice with experimental SLE and a reduction in IL 2 , IL 4 and interferon gamma ( INF gamma ) levels as compared with the levels detected in healthy controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
T lymphocytes and monocytes show important changes in the expression of cytokines like interleukin 1 , interleukin 2 , interferon gamma and tumor necrosis factor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation with interleukin 1beta , tumor necrosis factor alpha , or interferon gamma increased surface levels and de novo synthesis of CD40L on all three cell types . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Several experimental studies have suggested a role for the cytokines tumor necrosis factor alpha , interleukins 1 and 6 , and interferon gamma as mediators of the process of cachexia , although conclusive data supporting a role in human disease are often lacking . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We used a range of 2 4 mg for rHu TNF alpha and 50 100 mg of melphalan . rIFN gamma was administered on days 2 and 1 ( 15 10 10 ( 6 ) IU ) subcutaneously and the same dose was injected in the arterial line during ILP . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro , a variety of immunomodulators , including tumor necrosis factor , interferon g , and the hematopoietic growth factors , enhance the killing of Candida albicans , Aspergillus fumigatus , and Cryptococcus neoformans . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Monocyte human leukocyte antigen class 2 and CD 14 antigen expression , tumor necrosis factor production , and plasma interferon gamma and neopterin did not differ in patients before treatment and controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of interleukins 10 and 13 , interferons alpha and gamma , granulocyte / macrophage growth stimulating factor ( GM CSF ) , tumor necrosis factor alpha ( TNF alpha ) , and transforming growth factor beta ( TGFbeta ) on the proliferation of the cells was investigated by using radioactively labelled uridine as tracer . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Frequent in vitro inducers of HLA DR expression and enhanced class 1 major histocompatibility expression , interferon gamma , and tumor necrosis factor alpha were not detected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , attempts to overcome chlamydial growth arrest by incubation of Chlamydia infected monocytes with tryptophan , and antibodies against alpha interferon , gamma interferon , or tumor necrosis factor , were all ineffective , suggesting that known mechanisms of growth inhibition do not hold in human monocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Addition of interferon gamma or granulocyte / macrophage colony stimulating factor to the T cell / monocyte cultures enhanced T cell induction of monocyte TNF alpha by threefold and ninefold , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pentoxifylline consistently inhibited the accumulation of both TNF and iNOS in a dose dependent manner whether the stimulus was bacterial lipopolysaccharide ( LPS ) , recombinant interferon gamma ( rIFN gamma ) , or LPS plus rIFN gamma . ^^^ Similarly , rIL 10 consistently reduced TNF production by cells stimulated with LPS , rIFN gamma , or LPS plus rIFN gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
HA production was not significantly increased when HSE cells were incubated with TNF alpha , IL 4 or interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of VCAM 1 is stimulated by cytokines , such as tumor necrosis factor , interleukin 1 , and interferon gamma , as well as by oxidatively modified lipoproteins . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Complete examination of 21 patients with IgA nephropathy included determination urine and serum IL 6 , TNF alpha and INF gamma levels by ELISA ( Luzernachen , Luzern Switzerland ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This capability was investigated by activating bronchoalveolar lavage cells and peripheral blood mononuclear cells in vitro to elicit the release of tumor necrosis factor alpha , interleukin 2 and interferon gamma , whilst simultaneously exposing them to varying pharmacological concentrations of ambroxol ( 10 , 1 , and 0 . 1 microM ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In an attempt to understand the mechanism behind these differences we examined age related differences in the phenotype profiles of MNC in parallel with the in vitro production of interleukin IL 6 , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFNg ) in neonates , children and adults . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After exposure to LPS , mRNA of interferon gamma , which sensitizes hepatocytes to TNF toxicity , is overexpressed , and mRNA levels of interleukin 10 , a TNF inhibitor , are decreased . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro , aprotinin was added to cultures of a murine lung epithelial cell line and was stimulated with cytomix , a combination of TNF , interleukin 1 , and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Reverse transcriptase polymerase chain reaction analyses demonstrated intragraft expression of mRNAs for interleukin ( IL ) 1 , IL 2 , IL 4 , IL 6 , tumor necrosis factor , interferon gamma , and transforming growth factor beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Exogenous TNF alpha and high doses of interferon gamma inhibited follicle metabolism , especially hormone secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In undepleted IBD cultures , TNF alpha , IL 1alpha and beta , and IL 10 were markedly up regulated by pokeweed mitogen stimulation ; IL 1alpha and beta and IL 10 were also up regulated by stimulation of interferon gamma and lipopolysaccharide in combination . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The human colonic epithelial cell line HT 29 can be induced by a combination of the cytokines interleukin ( IL ) 1alpha , tumor necrosis factor alpha , and interferon gamma to express the inducible form of nitric oxide synthase ( iNOS ; Kolios , G . , Brown , Z . , Robson , R . , Robertson , D . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Several other growth factors / cytokines , such as interferon gamma , fibroblast growth factors 2 ( FGF 2 ) and 7 ( FGF 7 ) , interleukin 1beta , and transforming growth factor beta , unlike TNF alpha , modulated neither the morphology nor the biglycan expression of EA . hy 926 cells under the conditions used in the experiments . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin 2 ( IL 2 ) appeared in the initial inflammatory phase ( day 14 ) , subsided in the maximum inflammatory phase ( day 19 ) and disappeared by the recovery phase ( day 25 ) . mRNA of IL 3 beta , interferon gamma INF gamma and tumor necrosis factor alpha ( TNF alpha ) were detected only in the maximum inflammatory phase and iNOS also appeared for several days at this time . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here we demonstrate the utility and efficacy of combining IPG 2 D gel electrophoresis with mass spectrometry to identify interferon gamma ( IFN ) and tumor necrosis factor ( TNF ) regulated proteins in ME 180 cervical carcinoma cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Corresponding plasma levels of TNF alpha and interferon gamma were measured by ELISA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Other ovarian agonists ( i . e . , TNF alpha , IGF 1 , interferon gamma , and insulin ) were without effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PURPOSE : To determine the efficacy of isolated limb perfusion ( ILP ) with tumor necrosis factor alpha ( TNF ) in combination with interferon gamma ( IFN ) and melphalan as induction therapy to render tumors resectable and avoid amputation in patients with nonresectable extremity soft tissue sarcomas ( STS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PURPOSE : Recent reports indicate that hyperthermic isolated limb perfusion using tumor necrosis factor , interferon gamma and melphalan is associated with response rates of greater than 90 % in patients with in transit metastatic melanoma . ^^^ The relative contribution of tumor necrosis factor and interferon gamma to melphalan requires further study . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In endothelium denuded segments of pulmonary artery , the inflammatory agennts tumor necrosis factor alpha , interleukin 1 beta , interferon gamma , and lipopolysaccharide stimulated the release of PGE 2 and 8 iso PGF 2 alpha , which were attenuated in both cases by the cyclo oxygenase inhibitor indomethacin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFNgamma ) and tumor necrosis factor alpha ( TNFalpha ) cooperate to induce the expression of many gene products during inflammation . ^^^ IFNgamma and TNFalpha synergistically induce expression of mRNAs encoding interferon regulatory factor 1 ( IRF 1 ) , intercellular adhesion molecule 1 , Mig ( monokine induced by gamma interferon ) , and RANTES ( regulated on activation normal T cell expressed and secreted ) in normal but not STAT 1 deficient mouse fibroblasts , indicating a requirement for STAT 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MHC class 1 molecule expression on mesangial cells was significantly stimulated by interferon gamma , tumor necrosis factor alpha and interleukin 1beta , but not by interleukin 6 , in a dose dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Thrombocytopenia and complement activation under recombinant TNF alpha / IFN gamma therapy in man . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Soluble tumor necrosis factor ( TNF ) alpha , interleukin ( IL 1 beta , IL 2 , 6 , 8 , 10 , 12 , and interferon gamma were studied by enzyme linked immunosorbent assay . ^^^ TNF alpha , IL 1 beta , IL 2 , and interferon gamma were rarely found in FxSTH serum or fracture patient plasma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Toxoplasma gondii specific murine CD4+ T cell clone 3Tx9 belongs to the Th 1 subtype by virtue of secreting high levels of interleukin ( IL ) 2 , interferon gamma and tumor necrosis factor without producing IL 4 and IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We measured blood cellularity ( white blood cells and total lymphocytes ) , the total number and percentage of lymphocyte subsets ( CD 2 , CD 3 , CD 4 , CD 8 , and CD 19 ) , natural killer ( NK ) cell activity , cytokines ( interleukin 1 beta , interleukin 2 , interleukin 6 , granulocyte macrophage colony stimulating factor , tumor necrosis factor alpha , and interferon gamma ) , and soluble interleukin 2 receptors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The concentration of nitrites in the hepatocyte supernatant rose from 14 . 70+ / 3 . 55 micromol 10 l ( 1 ) to 150 . 50+ / 45 . 55 micromol 10 l ( 1 ) in the presence of a combination of interleukin 1beta , TNF alpha and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Probably , tumor necrosis factor and interferon gamma have a depressor effect on bone formation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
An inducible isoform of NO synthase ( iNOS ) catalyses the production of NO and is activated by lipopolysaccharide and certain T helper ( h ) 1 cytokines , including interferon gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Ag85A and Ag85B encoding plasmids induced a robust Th 1 like response towards native Ag 85 , characterized by elevated levels of interleukin ( IL ) 2 , interferon gamma , and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In IL 6 deficient ( IL 6 / ) mice , pneumonia was associated with higher lung levels of the proinflammatory cytokines tumor necrosis factor alpha , IL 1beta , and interferon gamma and of the antiinflammatory cytokine IL 10 than in wild type ( IL 6+ / + ) mice ( all P < . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The rp120cd peptide significantly increased the expression of CD 16 ( Fc gamma receptor type 3 ) and TNF receptor type 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The dominant pattern was one of high level secretion of interferon gamma and tumor necrosis factor ( TNF ) , with variable production of interleukin ( IL ) 2 , and little or none of the type 2 LK IL 4 , IL 5 , IL 6 , and IL 10 . ^^^ A small subset of gamma delta clones displayed a novel pattern , in which the only LK produced in substantial quantity were TNF and variable amounts of IL 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
No significant difference in serum levels of TNF and interferon gamma was found between infected wild type and Tnfr1null or Tnfr2null mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment with tumor necrosis factor and interferon gamma caused a 50 % decrease in the TER and a striking fragmentation of the basal , continuous interendothelial cell zonula occludens 1 protein ( ZO 1 ) distribution determined by immunofluorescence . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha , interleukin 1 , interleukin 4 and interferon gamma play a role in atopic inflammation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Positive cytotoxic T lymphocyte recall and cytokine ( interferon gamma and tumor necrosis factor alpha ) responses were detected for all peptides ; all were recognized in the context of more than one HLA class 1 molecule ; and at least 12 of the 17 were recognized in the context of all HLA alleles studied . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of arachidonic acid ethanolamide ( anandamide ) , palmitoylethanolamide and delta 9 tetrahydrocannabinol on the production of tumor necrosis factor alpha ( TNF alpha ) , interleukin 4 , interleukin 6 , interleukin 8 , interleukin 10 , interferon gamma , p 55 and p 75 TNF alpha soluble receptors by stimulated human peripheral blood mononuclear cells as well as [ 3H ] arachidonic acid release by non stimulated and N formyl Met Leu Phe ( fMLP ) stimulated human monocytes were investigated . ^^^ Anandamide was shown to diminish interleukin 6 and interleukin 8 production at low nanomolar concentrations ( 3 30 nM ) but inhibited the production of TNF alpha , interferon gamma , interleukin 4 and p 75 TNF alpha soluble receptors at higher concentrations ( 0 . 3 3 microM ) . ^^^ Palmitoylethanolamide inhibited interleukin 4 , interleukin 6 , interleukin 8 synthesis and the production of p 75 TNF alpha soluble receptors at concentrations similar to those of anandamide but failed to influence TNF alpha and interferon gamma production . ^^^ TNF alpha , interleukin 6 and interleukin 8 synthesis was maximally inhibited by 3 nM delta 9 tetrahydrocannabinol but stimulated by 3 microM delta 9 tetrahydrocannabinol , as was interleukin 8 and interferon gamma synthesis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine levels ( tumor necrosis factor alpha and interferon gamma ) measured 2 hr after the first dose were three to six times higher in patients treated with OKT 3 than in those treated with T10B9 ( P < 0 . 005 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Of all the other cytokines tested ( IL 1 , IL 2 , IL 4 , IL 6 , IL 10 , interferon gamma ( IFNgamma ) , leukemia inhibitory factor ( LIF ) , and tumor necrosis factor alpha ( TNFalpha ) only IL 1 strongly enhanced proliferation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Production of interleukin ( IL ) 1beta , IL 6 and tumour necrosis factor ( TNF ) alpha were induced with lipopolysaccharides ( LPS ) , and that of IL 2 and interferon ( IFN ) gamma with staphylococcal enterotoxin B ( SEB ) and phytohaemagglutinin ( PHA ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Remaining without influence on interferon gamma and interleukin 2 expression , ( R ) PMPA dramatically stimulated in a concentration and time dependent manner the secretion of tumor necrosis factor alpha ( TNF alpha ) and interleukin 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
As reported herein , a proinflammatory cytokine gene program that includes C 10 C and C C chemokines [ interleukin 8 ( IL 8 ) , growth related ( GRO ) alpha , GRO beta , GRO gamma , epithelial neutrophil activating peptide 78 ( ENA 78 ) , and RANTES ] and the cytokines tumor necrosis factor alpha ( TNF alpha ) and macrophage colony stimulating factor was upregulated in human hepatocytes after stimulation with IL 1 alpha or TNF alpha or bacterial invasion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When the effect of pro inflammatory cytokines was investigated , tumor necrosis factor alpha , but not interleukin 1 or interferon gamma , promoted an important degradation of IkappaBbeta followed by an increase in the mRNA at 1 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Pancreatic ascites was obtained from rats after induction of bile salt pancreatitis , cultured , and assayed for IL 1 , TNF , IL 6 , IL 8 , IL 10 , interferon gamma , and endotoxin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Functionally mature Vgamma9Vdelta2 T cells display a potent natural killer ( NK ) like cytotoxic activity , share with NK cells the expression of inhibitory receptors for HLA class 1 molecules , and release a plethora of cytokines , most notably interferon gamma and tumor necrosis factor alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN beta 1b also inhibits release of lymphotoxin , tumor necrosis factor , and interferon gamma , at least in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Reverse transcription polymerase chain reaction analyses revealed continuous intragraft expression of messenger RNA for interleukin 1 , interleukin 2 , interleukin 4 , interleukin 6 , tumor necrosis factor , interferon gamma , and transforming growth factor beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of MIC 1 mRNA in monocytoid cells is up regulated by a variety of stimuli associated with activation , including interleukin 1beta , tumor necrosis factor alpha ( TNF alpha ) , interleukin 2 , and macrophage colony stimulating factor but not interferon gamma , or lipopolysaccharide ( LPS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In 55 patients treated with the combination of high dose rTNF alpha + interferon gamma + melphalan an overall objective response rate of 87 % with 36 % complete responses was observed ; it was 81 % and 28 % , respectively , in a group treated with TNF alpha and melphalan ( n = 85 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study we demonstrate that interleukin 8 ( IL 8 ) , gamma immune protein 10 ( gammaIP 10 ) and tumour necrosis factor alpha ( TNF alpha ) all induce a significant chemokinetic response of human B lymphocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Production of interferon ( IFN ) gamma , tumor necrosis factor alpha ( TNF alpha ) , and interleukin ( IL ) 6 was performed on NK enriched peripheral blood mononuclear cells ( PBMC ) after stimulation with IL 2 and IFN alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The third enzyme , inducible NO synthase , is capable of producing large amounts of NO once induced by mediators such as interleukin ( IL ) 1 , IL 2 , IL 6 , tumour necrosis factor ( TNF ) alpha , and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neither the permissive ( i . e . allogeneic lymphocyte stimulation ) nor the non permissive ( i . e . interferon gamma + lipopolysaccharide ) activation condition had any effect on the capacity of alveolar macrophages from lung cancer patients to express mRNA for IL 1 alpha , IL 1 beta or TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found that , following C . neoformans challenge , PCA 2 treated but not saline treated mice exhibited ( a ) delayed brain colonization , ( b ) enhanced median survival times , ( c ) massive local immune reaction consisting of abundant astrocytes , microglial and inflammatory cells , and ( d ) a peculiar trend of cytokine gene expression , including high steady state levels of interleukin ( IL ) 1 beta and tumor necrosis factor alpha transcripts , fluctuating levels of interferon gamma and inducible nitric oxide synthase mRNA and lately detectable IL 6 gene expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It was induced by TNF and interferon gamma and depended on LFA 1 expressed on the platelet surface and ICAM 1 expressed on MVECs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of transfected HepG 2 cells with the cytokines interleukin 1beta , tumor necrosis factor alpha , and interferon gamma repressed promoter activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The molecular basis for the well known synergistic biological effects of tumor necrosis factor alpha ( TNFalpha ) and interferon gamma ( IFNgamma ) is still poorly understood . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We measured the effects of endotoxin , interferon gamma , and the combination of the two on production of tumor necrosis factor , interleukin 1 beta , and interleukin 6 in each line . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the mechanisms of eosinophil chemotaxis in atopic dermatitis by examining the effect of stimulation of epidermal keratinocytes ( KC ) by inflammatory cytokines , interferon gamma ( IFNgamma ) and / or tumor necrosis factor alpha ( TNF alpha ) on the production of eosinophil chemotactic factors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
STUDY DESIGN : Isolated rat hepatocytes were cultured with a cytokine mix consisting of tumor necrosis factor alpha , interleukin 1 beta , and interferon gamma in the presence or absence of the NO donor S nitroso N acetyl D , L penicillamine . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Subsequently , pancreatic ascites was cultured for bacteria and assayed for endotoxin and cytokines ( interleukin 1 , interleukin 6 , interleukin 8 , TNF alpha , or interferon gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The proinflammatory cytokines interleukin 1 ( IL 1 ) , IL 2 and interferon gamma can induce TNF production from target cells , including myocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , although the production of interleukin 2 , interferon gamma and tumor necrosis factor alpha increased during the first 48 hr of incubation , the values remained unchanged with respect to controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We show here that CP 10 is induced in murine endothelioma cell lines ( bEnd 3 , sEnd 1 , and tEnd 1 ) after activation with LPS and interleukin 1 ( IL 1 ) but not tumor necrosis factor alpha ( TNFalpha ) or interferon gamma ( IFNgamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The up regulation of ICAM 1 , in turn , may be due to high circulating levels of interferon gamma and / or TNF alpha . ^^^ After cytocentrifugation , the samples were prepared by the avidin biotin immunoperoxidase technique in order to detect the presence or absence of ICAM 1 , interferon gamma receptor and TNF alpha receptor ( p 80 ) on the tubular cells . ^^^ RESULTS : In nonrejecting patients , the tubular cells expressed the interferon gamma receptor but not ICAM 1 or the TNF alpha receptor . ^^^ The tubular cells expressed ICAM 1 and the TNF alpha receptor but not the interferon gamma receptor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Passive immunization with anti IL 12 serum intraperitoneally before LPS resulted in a marked reduction in plasma levels of tumor necrosis factor and interferon gamma . ^^^ Neutralization of tumor necrosis factor or interferon gamma in animals overexpressing IL 12 resulted in significant reductions in LPS induced mortality , suggesting that the mechanism whereby IL 12 increases LPS induced mortality is primarily mediated by the enhancement of these cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We tested the hypothesis that a mixture of tumor necrosis factor alpha , interleukin 1 alpha , and interferon gamma block the anabolic effects of IGF 1 in skeletal muscle . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum interferon gamma and tumor necrosis factor alpha levels postinfection were equally elevated in both mouse strains . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of gastric cells with tumor necrosis factor alpha , a receptor activating CD95 / APO 1 / Fas antibody , and interferon gamma markedly potentiated H . pylori induced DNA fragmentation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Analysis by bioassay and enzyme linked immunosorbent assay of bile showed the presence of tumor necrosis factor and interferon gamma , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Low levels of tumor necrosis factor ( TNF ) alpha and interferon gamma , high levels of interleukin ( IL ) 1beta , IL 6 , and IL 8 , and the presence of IL 10 were documented . ^^^ There were no significant differences in levels of TNF alpha and interferon gamma for CM and control patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of T98G cells with interferon gamma or interleukin 1 beta increased the expression levels of ADM mRNA and the IR ADM concentrations in the cultured media , whereas tumor necrosis factor alpha decreased them in a dose dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) , interleukin 1beta ( IL 1beta ) , and interferon gamma ( INF gamma ) have inhibitory effects on thyroid function both in vivo and in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of hepatocytes with lipopolysaccharide ( LPS ) and cytokine mixture ( interferon gamma and tumor necrosis factor alpha ) not only results in NO production but also causes cellular respiration suppression and cell death in hepatocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In cultured human smooth muscle cells from coronary arteries and saphenous veins , tumor necrosis factor alpha ( TNF alpha ) and interleukin 1 beta ( IL 1 beta ) induced the expression of intercellular adhesion molecule ( ICAM 1 ) and vascular cell adhesion molecule ( VCAM 1 ) , whereas interferon gamma ( INF gamma ) selectively stimulated the expression of ICAM 1 . ^^^ Basal and stimulated cAMP levels , measured by radioimmunoassay , did not differ in TNF alpha and IFN gamma treated cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Sera from patients in crisis had normal levels of granulocyte colony stimulating factor , granulocyte macrophage colony stimulating factor , interferon gamma , TNFalpha , interleukin 1 ( IL 1 ) , IL 6 , or IL 8 and did not modify the adherence of PMNs or their expression of CD 64 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The contribution of E and P selectin in the rat to the migration of polymorphonuclear leucocytes ( PMNL ) and monocytes to acute dermal inflammation induced by a chemoattractant ( C5ades Arg ) or endothelial cell activating agents [ lipopolysaccharide , tumour necrosis factor alpha ( TNF alpha ) , alpha thrombin and interferon gamma ] administered intradermally was investigated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although IL 2 induced proliferation of these cytotoxic T lymphocytes ( CTLs ) , production of interferon gamma and tumor necrosis factor alpha required stimulation of the CTL with E . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neither SpeA nor SpeC ( with or without rIFN gamma ) reproducibly induced TNF production by these murine macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Three categories of potential regulators were tested ; the lipopolysaccharide ( LPS ) and the yeast extract zymosan ; follicle stimulating hormone ( FSH ) , testosterone and dexamethasone ; tumor necrosis factor alpha ( TNFalpha ) , interferon gamma ( IFNgamma ) and the nerve growth factor beta ( NGFbeta ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After two to seven passages , cultured orbital fibroblasts were incubated for 3 days with interferon gamma or tumor necrosis factor alpha in the presence of nicotinamide . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Most remarkable , it does not stimulate T lymphocyte proliferation in the absence of tumor cells and , moreover , does not induce CD 25 up regulation and the secretion of potentially toxic lymphokines such as tumor necrosis factor alpha , interleukin 6 and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
High plasma concentrations of the inflammatory cytokines tumor necrosis factor alpha and interferon gamma were observed at this dose . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Culturing hepatocytes with a combination of tumor necrosis factor alpha , interferon gamma , and interleukin 1 beta plus lipopolysaccharide resulted in an induction of nitric oxide synthase and concomitant inhibition of both hepatic gluconeogenesis and glycogenolysis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The present study was designed to determine whether the cytokines tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( INF gamma ) , and interleukin 1beta ( IL 1beta ) stimulate tetrahydrobiopterin synthesis by increasing expression of GTP cyclohydrolase 1 mRNA in endothelial cells . ^^^ After incubation with TNF alpha ( 2 microg / mL ) , INF gamma ( 200 U / mL ) , and IL 1beta ( 5 U / mL ) for 24 hours , significantly increased expression of GTP cyclohydrolase 1 mRNA was detected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here we describe altered ligands for mature mouse CD 4 ( + ) T helper 1 cells that lead to T cell apoptosis by the selective expression of Fas ligand ( FasL ) and tumor necrosis factor ( TNF ) without concomitant IL 2 , IL 3 , or interferon gamma production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In summary , our study showed that enhanced binding of neutrophils to epidermal sections can be achieved by prestimulation of both the keratinocytes with interferon gamma and the neutrophils with fMLP , TNF alpha and PAF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Lipopolysaccharide ( LPS ) up to 250 pg / ml was used for the stimulation of monocytes for measuring the production of tumour necrosis factor alpha ( TNF alpha ) , interleukin 6 ( IL 6 ) and IL 12 , while the anti CD 3 ( 1 microgram / ml ) and anti CD 28 ( 5 micrograms / ml ) combination was used for T cell stimulation with the measuring of IL 4 and interferon gamma ( INF gamma ) production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The treatment of glial cells with either LPS alone ( microglia ) or a combination of LPS and interferon gamma ( astrocytes ) resulted in an induced production of NO and TNFalpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Rat Sertoli cells express an inducible nitric oxide synthase isoform ( iNOS ) in response to the combined addition of the cytokines interferon gamma ( IFNgamma ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 1alpha ( IL 1alpha ) and lipopolysaccharides ( LPS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition to vascular cells , many activated immune cells , mainly macrophages and T cells , are present in atherosclerotic lesions , where these cells produce biologically active substances such as the proinflammatory cytokines tumor necrosis factor , interleukin 1 ( IL 1 ) , and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Analysis of cytokine mRNA expression in lung tissue indicated that steady state levels of interleukin ( IL ) 1beta , tumor necrosis factor ( TNF ) alpha , interferon gamma , and IL 12 were significantly elevated in lungs of GVHD mice at 3 wk post BMT compared with untreated controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , marked differences were not found in the levels of interleukin 3 ( IL 3 ) , tumor necrosis factor alpha ( TNF alpha ) , stem cell factor ( SCF ) , granulocyte / macrophage colony stimulating factor ( GM CSF ) , or interferon gamma ) ( IF gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CD 54 ( intercellular adhesion molecule 1 , ICAM 1 ) surface expression on the blasts in ten cases of acute lymphoblastic leukemia ( ALL ) and its up regulation by human recombinant interferon gamma ( IFNgamma ) and / or tumor necrosis factor alpha ( TNFalpha ) were studied in a serum free culture system . ^^^ Effect of stimulation by interferon gamma and tumor necrosis factor alpha on CD 54 expression and homotypic aggregation of blasts of acute lymphoblastic leukemias . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pretreatment with interferon gamma and tumor necrosis factor alpha reduces the production of active TGFbeta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , on stimulation with lipopolysaccharide and / or interferon gamma , spleen cells from malaria cured mice showed an increased capacity to produce tumor necrosis factor , interleukin 6 , and reactive nitrogen intermediates as compared with spleen cells from naive mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increased activities of interferon ( IFN ) gamma , tumor necrosis factor ( TNF ) alpha , and interleukin ( IL ) 2 have been shown in the inflamed mucosa of inflammatory bowel disease . ^^^ Induction of major histocompatibility complex class 2 antigens on human colonic epithelium by interferon gamma , tumor necrosis factor alpha , and interleukin 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A durable increase of natural killer activity retained by poeripheral blood mononuclear cells was demonstrated in these patients and was paralleled by increased serum levels of interferon gamma and tumor necrosis factor a without changes of circulating interleukin 1d . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Reactivation was also observed when myeloid cells were carried in the presence of fibroblast conditioned medium or medium supplemented with certain cytokines ( interferon gamma , tumor necrosis factor alpha , interleukin 4 , or granulocyte macrophage colony simulating factor ) , suggesting that cell differentiation pathways act as determinants of reactivation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have previously demonstrated that hepatitis B virus ( HBV ) replication and gene expression are abolished in the livers of HBV transgenic mice by cytotoxic T lymphocytes ( CTLs ) and during lymphocytic choriomeningitis virus ( LCMV ) infection , stimuli that trigger the production of alpha / beta interferon , gamma interferon , and tumor necrosis factor alpha in the liver . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha and IL 1beta stimulated PTHrP expression in synoviocytes , while dexamethasone and interferon gamma , agents with some therapeutic efficacy in the treatment of RA , inhibited PTHrP release . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , synergy between rIFN gamma and YS Tang was mainly dependent on YS Tang induced tumor necrosis factor alpha ( TNF alpha ) secretion . ^^^ These results suggest that the capacity of YS Tang to increase NO production from rIFN gamma primed mouse peritoneal macrophages is the result of YS Tang induced TNF alpha secretion . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Results indicate that fumonisin B 1 toxicity may involve secretion of TNF alpha by TNF alpha producing cells without altering interleukin 1alpha or interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Antibodies to tumor necrosis factor alpha , interleukin 1 alpha , interferon gamma ( 7 micrograms / day ) , and indomethacin ( 50 micrograms / day ) were administered by microinfusion pumps for 7 days and the PGF was harvested . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Detectable levels of interleukin ( IL ) 6 , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma , but not of IL 2 , IL 4 or transforming growth factor beta 2 , were found in supernatants from cultured cells in both study groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Identification of a human enterocyte lipoxin A 4 receptor that is regulated by interleukin ( IL ) 13 and interferon gamma and inhibits tumor necrosis factor alpha induced IL 8 release . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In these mice , we examined the histology of the stomach , antibody titers against H . pylori , and serum levels of cytokines ( IL 4 , IL 10 , TNF alpha , IL 2 and Interferon gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha , interleukin 1beta , and dexamethasone elevated AM secretion , whereas transforming growth factor beta 1 and interferon gamma suppressed it in these three fibroblasts . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 2 gene transduced NK 92 cells had an in vitro cytotoxicity against tumor targets that was significantly higher than that of parental cells and secreted interferon gamma ( IFNgamma ) and tumor necrosis factor alpha ( TNFalpha ) in addition to IL 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using this approach , the median survival of tumor bearing nude mice was prolonged by the administration of a human tumor necrosis factor alpha ( TNF alpha ) gene inserted into a eukaryotic strong expression vector ( pcagTNF alpha ) and exogenously added interferon gamma ( INF gamma ) ( 72 + / 3 days : mean + / s . e . ) . ^^^ By contrast , survival was 36 + / 3 , 39 + / 3 and 46 + / 4 days in group receiving the TNF alpha gene inserted in the reverse orientation ( pcagTNF alpha R ) and normal saline ( NS ) , pcagTNF alpha R and INF gamma and pcagTNF alpha and NS , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When islets were isolated from rat pancreata 20 22 days after birth , islet cell viability , DNA synthetic rate , and insulin release were reduced after incubation with interleukin 1beta , tumor necrosis factor , or interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Plasma was collected in EDTA tubes on ice , immediately centrifuged at 4 degrees C and stored frozen at 80 degrees C ; samples were measured by immunoradiometric assays ( Medgenix Fleurus , Belgium ) for tumor necrosis factor alpha ( TNF ) , interleukin 1 , 2 and 6 ( IL 1 , IL 2 , IL 6 ) and interferon gamma ( IFN ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF production was enhanced when the cultures were simultaneously stimulated with canine recombinant , or supernatant derived , interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These cytokines , namely , tumor necrosis factor , interleukin 1 , and interferon gamma , induce production and release of bFGF , which stimulates angiogenesis and spindle cell growth in an autocrine fashion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A specific combination of cytokines , i . e . , IL 1 ( 1 ng / ml ) + TNFalpha ( 10 ng / ml ) / interferon gamma ( IFNgamma ) ( 200 u / ml ) , induced a dramatic neuronal cell injury in the neuron / glia cultures , and its cytotoxic profile was very similar to that seen with the LPS / IFNgamma induced neuron injury . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The combination of melphalan with tumour necrosis factor alpha ( TNFalpha ) and interferon gamma ( IFNgamma ) in isolated limb perfusion ( TIM ILP ) gives around 80 % CR . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The proliferation of T cells and their release of cytokines such as IL 2 and interferon gamma are also suppressed by 1 , 25 D 3 , partly as a result of the reduced production of T cell activating cytokines ( interleukin 1 alpha , tumor necrosis factor alpha ) , but also because of a direct effect on the T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma , a Th 1 cytokine , was much more elevated in the Listeria stimulated nodes , but TNF alpha ( also a Th 1 cytokine ) was more elevated in Serratia infected nodes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Free flow electrophoresis ( FFE ) has been applied to the separation and purification of a variety of proteins and polypeptides : bee venom , tumor necrosis factor , interleukin 1beta , interferon gamma and superoxide dismutase . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
An initial challenge led to a transient increase in tumor necrosis factor alpha production followed predominately by the T helper ( Th ) 1 cytokine , interferon gamma . ^^^ An irritant response induced by chronic exposure of mouse skin to phorbol ester did not reveal any significant interferon gamma or interleukin 4 response but was characterized by a tumor necrosis factor alpha response that correlated with the inflammatory response . ^^^ These observations suggest that the major source of interferon gamma and interleukin 4 in the oxazolone response may be the infiltrating lymphocytes ; whereas the tumor necrosis factor alpha may result from the local irritation seen with both oxazolone and phorbol ester . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Many of these cytokines , including interleukin 1 ( IL 1 ) , IL 2 , IL 6 , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( INF gamma ) are synthesized in the adrenal gland by both parenchymal cells and resident macrophages , and the release of some of them ( e . g . , IL 6 and TNF alpha ) is regulated by the main agonists of steroid hormone secretion ( e . g . , ACTH and angiotensin 2 ) and bacterial endotoxins . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Finally , using neutralizing antibodies or cytokine inhibitors we provide evidence that this inhibition of extracellular matrix production mediated by T cell contact was partially due to additive effects of T cell membrane associated interferon gamma , tumor necrosis factor alpha , and interleukin 1alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
SCF potentiates lipopolysaccharide stimulated , but attenuates interferon gamma TFNalpha mediated expression of the mRNAs of IL 1beta and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin 2 , Interleukin 4 , Interleukin 10 , Interferon gamma , Interleukin 12 , Tumor Necrosis Factor alpha values were measured in serum at diagnosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Simultaneous activation with the proinflammatory cytokines , interferon gamma , interleukin 1beta and TNF alpha , increased NO synthesis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Elevated levels of interferon gamma , tumor necrosis factor alpha , interleukins 2 , 4 , and 5 , but not interleukin 10 , are present in recurrent aphthous stomatitis . ^^^ For part 1 , interleukins ( IL ) 2 , 4 , 5 , and 10 , interferon gamma , and tumor necrosis factor alpha were measured . ^^^ RESULTS : In part 1 , elevated levels of IL 2 , interferon gamma , and tumor necrosis factor alpha mRNAs were detected in RAS lesions , consistent with a cell mediated immune response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The rat adrenal cortex produces the cytokines interleukin 6 , tumor necrosis factor , interleukin 1beta , interleukin 1alpha , macrophage migration inhibitory factor , interferon gamma inducing factor , and transforming growth factor beta 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Plasma levels of soluble interleukin 2 receptor , interferon gamma , interleukin 6 , and TNF alpha receptor decreased over the 42 day study period . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
ALP mRNA was constitutively expressed in keratinocytes and the expression was not significantly affected by TNF alpha or Interferon gamma stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Type 1 cytokines interleukin 2 ( IL 2 ) , interferon gamma ( IFNgamma ) , and tumor necrosis factor beta ( TNFbeta ) , dominate over an immunoregulatory ( suppressor ) Th 2 subset of T cells and their cytokine products , i . e . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of ICAM 1 is induced by cytokines like Interleukin 1 , TNF alpha or interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In comparative studies of coccidiosis in chickens , in vivo and in vitro studies revealed that interferon gamma , tumor necrosis factor and transforming growth factor beta are induced following Eimeria infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To clarify this issue , mRNAs and / or protein levels of TNF alpha and cytokines [ interleukin ( IL ) 6 , IL 10 , transforming growth factor beta ( TGF ) beta , IL 12 , and interferon gamma ] that regulate its biological activity were measured in sera , liver , and adipose tissues of rats that had developed hepatic steatosis after consuming ethanol containing diets for 6 weeks . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Regional administration of high doses of tumor necrosis factor ( TNF ) , interferon gamma ( IFNgamma ) and melphalan to patients with advanced cancers of the limbs , results in rapid and specific tumor necrosis , while the normal adjacent tissues remain unaffected . ^^^ Pulse treatment of human vascular endothelial cells with high doses of tumor necrosis factor and interferon gamma results in simultaneous synergistic and reversible effects on proliferation and morphology . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We synthesized eight peptides with alanine replacement of all residues in the SEB sequence 131 138 and tested them for the capacity to inhibit both SEB induced proliferation of human lymphomonocytes and the production of tumor necrosis factor alpha and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Even if TNF alpha is of importance to the developing process of ICC xenograft rejection , other cytokines , i . e . , interferon gamma , might efficiently substitute for the lack of TNF alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Recently , we found that priming microglia with interferon ( IFN ) gamma or tumor necrosis factor ( TNF ) alpha resulted in an enhanced production of superoxide anion , a reactive oxygen intermediate that may be pathogenic during brain inflammation . ^^^ Maximal inhibition of microglial cell superoxide generation by U 50 , 488 was observed at 10 nM for the priming effect of interferon gamma and at 1 microM for tumor necrosis factor alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found that mRNAs of tumor necrosis factor alpha ( TNFalpha ) and interleukin 1alpha ( IL 1alpha ) were considerably expressed in both PSK treated and phosphate buffered saline treated tumors , and that the mRNA expression and protein level of interferon gamma ( IFNgamma ) increased in the tumor tissues treated with PSK . ^^^ Polysaccharide K induces Mn superoxide dismutase ( Mn SOD ) in tumor tissues and inhibits malignant progression of QR 32 tumor cells : possible roles of interferon alpha , tumor necrosis factor alpha and transforming growth factor beta in Mn SOD induction by polysaccharide K . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The transduced cells secreted 2 4 ng / ml of latent TGF beta 1 into their culture medium , whereas control cells secreted barely detectable amounts . mRNA profiles for tumor necrosis factor , lymphotoxin , and interferon gamma were similar before and after transduction ; interleukin 4 and 10 were absent . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma and alpha ( IFN ) , colony stimulating factor 1 , interleukin 6 ( IL 6 ) and tumor necrosis factor alpha ( TNF alpha ) all induce C 1 INH synthesis in a variety of cell types . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Cyclosporine was found to ameliorate arthritis , suppress interferon gamma ( IFNgamma ) production by CD4+ T cells , and reduce TNFalpha expression in arthritic joints . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
As well , there is evidence that TNFalpha and interferon gamma induce and EGF inhibits apoptosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Over the first 6 h , there were significant increases in plasma tumor necrosis factor alpha ( TNFalpha ; from 114+ / 54 pg / ml [ mean+ / SEM ] to 236+ / 161 pg / ml , p < 0 . 05 ) and interleukin ( IL ) 6 ( 260+ / 121 pg / ml to 445+ / 149 pg / ml , p < 0 . 05 ) but not in interferon gamma ( IFNgamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of four candidate therapeutic genes , namely human granulocyte macrophage colony stimulating factor , tumor necrosis factor alpha , interleukin 2 , and interferon gamma , was detectable for 4 to 7 days in CD34+ cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Monkeys immunized with either P . falciparum MSP r190L or synthetic 190L peptides expressed predominantly the type 1 cytokines ( IL 1 beta , IL 12 , interferon gamma , TNF alpha , TNF beta ) characteristic of splenic , cell mediated activity with macrophage activation and nitric oxide production . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Whole blood samples from 14 dogs with CDV infection and three dogs suffering from non distemper diseases were examined for mRNA of pro inflammatory cytokines such as interleukin 1beta ( IL 1 ) , interleukin 6 ( IL 6 ) , interleukin 8 ( IL 8 ) , interleukin 12 ( IL 12 ) , tumor necrosis factor alpha ( TNF ) , interferon gamma ( IFN ) , and the anti inflammatory transforming growth factor beta 1 ( TGF ) using reverse transcription polymerase chain reaction ( RT PCR ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Regulation of interleukin 12 by interleukin 10 , transforming growth factor beta , tumor necrosis factor alpha , and interferon gamma in human monocytes infected with Mycobacterium tuberculosis H37Ra . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Resistance was associated with unimpaired innate antifungal activity of pulmonary phagocytic cells , concomitant with high level production of tumor necrosis factor ( TNF ) alpha and interleukin ( IL ) 12 and the presence of interstitial lymphocytes producing interferon gamma and IL 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of synoviocytes with interferon gamma resulted in the release of TNF alpha in response to chlamydial infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In one horse , there was up regulation of interferon gamma , tumor necrosis factor alpha ( TNF alpha ) and interleukin 2 mRNAs , while in the second horse , expression of only TNF alpha mRNA was up regulated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This report shows that murine granulosa cells are initially resistant to antibody induced Fas mediated apoptosis , but will undergo apoptosis when cotreated with TNF and interferon gamma ( IFN ) or cycloheximide ( CX ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Th 1 responses are characterized by secretion of interleukin ( IL ) 2 , tumor necrosis factor ( TNF ) alpha , lymphotoxin , and interferon ( IFN ) gamma and are associated with delayed type hypersensitivity reactions , whereas Th 2 responses , which are characterized by secretion of IL 4 , IL 5 , and IL 10 , have been associated with humoral immune responses and allergy . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma production also increased after infection with FIV whereas , TNFalpha expression remained constant . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A combination of TNFalpha with interferon gamma acted synergistically on the epithelial barrier . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Oligodendrocytes in multiple sclerosis brain may be under a direct attack by proinflammatory cytokines , particularly tumor necrosis factor alpha ( TNFalpha ) and interferon gamma ( IFNgamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
On the other hand , the serum levels of interferon gamma , tumor necrosis factor alpha , interleukin 6 , soluble human leukocyte antigen class 1 , and soluble thrombomodulin were significantly increased after bone marrow transplantation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Toxicity and antitumor activity of interferon gamma alone and in combinations with TNFalpha and melphalan in isolated limb perfusion in the BN 175 sarcoma tumor model in rats . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We show that the combination of tumor necrosis factor alpha and interferon gamma triggers the production of beta amyloid peptides and inhibits the secretion of soluble APPs by human neuronal and extraneuronal cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Rat liver slices were maintained in culture for 15 min to 24 h and examined for ICAM 1 expression by immunohistochemistry and Western blotting in basal conditions and after stimulation with 1000 IU / ml interferon gamma ( IFNgamma ) , 1000 IU / ml tumor necrosis factor alpha ( TNF alpha ) and 50 microg / ml endotoxin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunohistochemical staining was used to enumerate cytokine producing cells ( monokines : interleukin [ IL ] 1alpha , IL 1beta , IL 6 , and tumor necrosis factor [ TNF ] alpha ; lymphokines : interferon gamma , IL 2 , IL 4 , and TNF beta ) in tissues obtained at autopsy from subjects with HPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : We used competitive template polymerase chain reaction techniques to quantify transcript amounts for the constant region of the T cell receptor beta chain ; the cytokines , tumor necrosis factor alpha , interleukin ( IL ) 1beta , transforming growth factor beta , interferon gamma , IL 2 , IL 4 , IL 10 , and IL 15 ; and the cytotoxic T lymphocyte effector molecules , granzyme B , perforin , and Fas ligand . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MIC 1 is not expressed in resting macrophages but is induced by a number of different activation agents , including phorbol myristate acetate , interleukin 1 , tumor necrosis factor alpha , and macrophage colony stimulating factor but not by lipopolysaccharide or interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
No differences were detected in LPS induced serum levels of interleukin ( IL ) 1beta , tumor necrosis factor , macrophage inflammatory protein 1alpha , and interferon gamma in ob / ob mice compared with their own littermates . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When stimulated with LPS and interferon gamma , macrophages from MIF / mice showed diminished production of TNF alpha , normal IL 6 and IL 12 , and increased production of nitric oxide . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation with the exogenous cytokines interferon gamma and tumor necrosis factor alpha induced or significantly up regulated expression of several of the cytokines and chemokines ( i . e . , IL 6 , IL 8 , RANTES , and M CSF ) , as well as major histocompatibility complex ( MHC ) class 2 antigens , and membrane expression and shedding of the intercellular adhesion molecule 1 in all three cell lines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Extensive studies proved that these genes are critical for mediating cell death initiated by interferon gamma , and in some of the tested cases also cell death induced by Fas / APO 1 , TNF alpha , and a detachment from extracellular matrix . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Nevertheless , IL 18 may contribute to inflammation and fever because IL 18 is a potent inducer of tumor necrosis factor , chemokines , and interferon gamma production . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Amelioration of collagen induced arthritis and suppression of interferon gamma , interleukin 12 , and tumor necrosis factor alpha production by interferon beta gene therapy . ^^^ In vitro , supernatants from these engineered fibroblasts inhibited collagen induced interferon gamma secretion from lymph node cells , and reduced the levels of tumor necrosis factor alpha and interleukin 12 produced by lipopolysaccharide / IFNgamma treated bone marrow derived macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Th 1 type cytokines ( interleukin 2 ( IL 2 ) , tumour necrosis factor beta ( TNF beta ) , and interferon gamma ( INF gamma ) and Th 2 type cytokines ( IL 4 , IL 10 ) were measured by means of enzyme linked immunosorbent assays . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the role of anti TNF in the regulation of MHC class 2 antigens and cytokine mRNA expression of TNF , interferon gamma ( IFN ) , IL 2 , IL 4 , and IL 10 in cardiac allografts to elucidate its immunological mechanism . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The macrophage derived cytokines interleukin beta ( IL 1beta ) , and tumor necrosis factor alpha ( TNFalpha ) , and the T cell derived cytokine interferon gamma ( IFNgamma ) have been implicated to play an important role in early attack on islet cells during human islet transplantation ( ITx ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A reverse transcription competitive polymerase chain reaction ( RT cPCR ) method was developed to quantitate equine interleukin ( IL ) 1alpha , IL 1beta , IL 2 , IL 4 , IL 5 , IL 6 , IL 10 , IL 12 p 35 , IL 12 p 40 , interferon gamma ( INF gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and beta actin mRNA expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Following treatment with interleukin 1beta ( IL 1beta ) , human astrocytes demonstrated increased message and protein expression for MIP 1alpha , while other immune modulators such as interferon gamma ( IFN ) gamma , tumor necrosis factor alpha ( TNF alpha ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , lipopolysaccharide , or phorbol ester ( a protein kinase C activator ) did not induce MIP 1alpha expression in human astrocytes . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The production of interleukin 2 ( IL 2 ) , interferon gamma ( IFNgamma ) and tumor necrosis factor alpha ( TNFalpha ) was determined in T helper ( Th ) and cytotoxic T cells ( CTL ) as well as in naive and memory cells after stimulation with phorbol 12 myristate 13 acetate ( PMA ) and ionomycin under the influence of monensin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro , usual inducers of matrix metalloproteinases such as interleukin 1 , interferon gamma , or tumor necrosis factor alpha did not augment stromelysin 3 in vascular wall cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Frozen sections were stained with Giemsa and also immunostained for the presence of interleukin 2 , 4 , and 10 , tumor necrosis factor alpha ( TNF alpha ) , and interferon . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Whether p 38 and extracellular signal regulated kinase ( ERK ) mitogen activated protein kinase cascades are required for inducible nitric oxide synthase ( iNOS ) and tumor necrosis factor ( TNF ) accumulation in RAW 264 . 7 murine macrophages exposed to lipopolysaccharide ( LPS ) plus recombinant interferon gamma ( rIFN gamma ) was investigated . ^^^ Specific inhibitors of p 38 and extracellular signal regulated kinase mitogen activated protein kinase pathways block inducible nitric oxide synthase and tumor necrosis factor accumulation in murine macrophages stimulated with lipopolysaccharide and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study was designed to examine the kinetics of expression of 10 cytokines in experimentally induced murine periapical lesions , including bone resorptive [ IL 1alpha , tumour necrosis factor alpha ( TNFalpha ) , IL 6 , IL 11 ] , Th 1 type [ IL 2 , IL 12 , interferon gamma ( IFNgamma ) ] and Th 2 type ( IL 4 , IL 6 , IL 10 , IL 13 ) mediators . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Among the three inflammatory cytokines : TNF alpha , interleukin 1 , and interferon gamma , TNF alpha ( 100 units / ml ) gave the most induction of AR . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In response to endotoxin , CyCAP deficient mice overproduced interleukin 12 and interferon gamma systemically and tumor necrosis factor alpha locally ; these are proinflammatory molecules that also promote T helper 1 responses . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lipopolysaccarides and cytokines , such as tumor necrosis factor , interleukin 1 and interferon gamma , have been shown to induce iNOS in the endothelium , vascular smooth muscle cells , macrophages and different parenchymal cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PlGF induced activation of the SAPK signaling pathways protected trophoblast from growth factor withdrawal induced apoptosis , but it did not protect trophoblast from apoptosis induced by the pro inflammatory cytokines , interferon gamma and tumor necrosis factor alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
BACKGROUND : Hyperthermic isolated limb perfusion ( HILP ) with tumor necrosis factor alpha ( TNF alpha ) , interferon gamma , and melphalan has proved to be useful in the treatment of recurrent malignant melanoma and of locally advanced soft tissue sarcomas of the extremities . ^^^ PATIENTS AND METHODS : Fifteen patients with locally advanced primary , recurrent , or metastatic skin tumors of the extremities ( 12 with squamous cell carcinoma and 3 with Merkel cell carcinoma ) underwent HILP with TNF alpha , interferon gamma , and melphalan . ^^^ CONCLUSION : Based on our results , HILP with TNF alpha , interferon gamma , and melphalan should be considered as a limb saving treatment modality in patients with locally advanced nonmelanoma skin tumors of the extremities who would otherwise be candidates for ablative surgery . . ^^^ Hyperthermic isolated limb perfusion with tumor necrosis factor alpha , interferon gamma , and melphalan for locally advanced nonmelanoma skin tumors of the extremities : a multicenter study . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
By immunohistologic staining , the cells in the perivascular and vascular infiltrates in the kidneys of older PN mice were shown to produce interleukin 4 ( IL 4 ) , IL 6 , and IL 10 , but not IL 2 , interferon gamma , transforming growth factor beta , tumor necrosis factor alpha , or IL 1beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effect of interferon gamma ( IFN ) and the inflammatory cytokines tumour necrosis factor alpha ( TNF ) and interleukin 1alpha ( IL 1 ) on micro and macrovascular endothelial cell ( EC ) proliferation and migration was analysed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
At study entry , TNF alpha and IL 6 levels were detected in six and seven patients respectively ; IL 1 levels were low and interferon gamma was undetectable . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To determine whether common skin diseases associated with human immunodeficiency virus ( HIV ) were distinguishable based on the pattern of serum cytokine expression , we studied patients with psoriasis , pruritus , and Kaposi ' s sarcoma ( KS ) for levels of tumor necrosis factor ( TNF ) alpha , interferon gamma ( IFN y ) , interleukin ( IL ) 10 , and IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Th 1 lymphocytes seem to orchestrate the inflammation through the production of proinflammatory cytokines such as interferon gamma , interleukin 1 beta , and tumor necrosis factor ( TNF ) alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There was no correlation between these values and patient outcome or levels of plasma TNF alpha or serum interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Culture supernatants derived from A 549 cells exposed to TNF alpha and interferon gamma demonstrated detectable levels of IL 5 protein , and immunostaining of bronchial biopsies obtained from healthy human airways revealed the presence of IL 5 protein localized to the bronchial epithelium . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunohistological staining of cartilage sections was performed using antibodies for the following cytokines / growth factors : tumor necrosis factor alpha ( TNF alpha ) , interleukin 1alpha ( IL 1alpha ) , IL 1beta , interferon gamma , IL 6 , IL 4 , IL 10 , transforming growth factor beta 1 ( TGF beta 1 ) , insulin like growth factor 1 ( IGF 1 ) , IGF 2 , platelet derived growth factor AA ( PDGF AA ) , and PDGF BB . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
At the 80th hour , submucosal macrophages were positive for TNFalpha and colonic production of IL1beta and interferon gamma was increased ; all these effects were reduced by octreotide treatment . ^^^ CONCLUSIONS : TNFalpha was expressed early by resident muscle cells , before staining of infiltrated immune cells and increased production of interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , synergy between rIFN gamma and UrCE was mainly dependent on UrCE induced tumor necrosis factor alpha ( TNF alpha ) secretion . ^^^ These results suggest that the capacity or UrCE to increase NO production from rIFN gamma primed mouse peritoneal macrophages is the result of UrCE induced TNF alpha secretion . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Dual mechanisms of action of interferon gamma in potentiating responses to LPS in mice : IL 1 , TNFalpha and IL 6 production in serum and hypothermia . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MAIN OUTCOME MEASURES : Blood levels were compared at baseline and after the exercise program had been completed for the following : spontaneous and phytohemagglutinin induced production of interleukin 1 alpha , tumor necrosis factor alpha , and interferon gamma ( atherogenic cytokines ) , and interleukin 4 , interleukin 10 , and transforming growth factor beta 1 ( atheroprotective cytokines ) by blood mononuclear cells ; lymphocyte phenotypes and mitogenic responses to phytohemagglutinin ; and serum C reactive protein levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To examine the production of hepatocyte growth factor ( HGF ) by human endometrial stromal cells ( ESC ) in vitro , concentrations of HGF in the culture media of ESC were measured after the addition of various amounts of 12 O tetradecanoylphorbol 13 acetate ( TPA ) , forskolin , lipopolysaccharide ( LPS ) , interleukin ( IL ) 1beta , IL 6 , IL 8 , tumor necrosis factor alpha ( TNFalpha ) , interferon gamma ( IFNgamma ) , or ethynylestradiol 17alpha using an ELISA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , TNFalpha , interferon gamma , IL 4 , IL 6 , and IL 10 were rarely found in the synovial infiltrates . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
BACKGROUND : The aim of the present study was to examine the production of interferon gamma , tumor necrosis factor alpha ( TNF alpha ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 6 ( IL 6 ) , IL 10 , IL 4 , IL 5 , IL 1 receptor antagonist ( IL 1RA ) , and prostaglandin E 2 in relation to the number of leukocytes in the blood of detoxified , chronic alcoholic patients without apparent liver disease ( AWLD ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In RGP IFN gamma Tg mice , serum concentrations of interferon gamma and tumour necrosis factor alpha ( TNF alpha ) were 50 and 6 times higher than those in their littermates , respectively , and glucose responsive insulin secretion decreased to one half the level of that in the littermates . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
VIP / PACAP inhibit the production of IL 12 , IL 6 , tumor necrosis factor alpha ( TNFalpha ) , and interferon gamma ( IFNgamma ) in vivo in endotoxemic mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increase of VEGF A production in T cells was promoted by inflammatory cytokines ( IC ) present in HIV TCM , including tumor necrosis factor alpha ( TNFalpha ) , interferon gamma ( IFNgamma ) , interleukin 1beta ( IL 1beta ) , and IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In parallel , transmembrane TNF expressing HeLa cells display high sensitivity to cycloheximide or interferon gamma , similar to untransfected cells treated with these agents in combination with sTNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OBJECTIVE : The aims of the present study were to examine the in vitro effects of lithium on the unstimulated and lipolysaccharide ( LPS ) + phytohemagglutinin ( PHA ) induced production of proinflammatory cytokines , such as interleukin 6 ( IL 6 ) , IL 8 , tumor necrosis factor alpha ( TNFalpha ) and interferon gamma ( IFNgamma ) , and negative immunoregulatory cytokines or proteins , such as IL 10 and the IL 1 receptor antagonist ( IL 1RA ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Ceramide , generated by the hydrolysis of sphingomyelin , mediates the actions of several cytokines such as tumour necrosis factor alpha ( TNF alpha ) interferon gamma and interleukin 1beta ( IL 1beta ) , including their inhibitory effect on tumour proliferation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma receptor mediated but not tumor necrosis factor receptor type 1 or type 2 mediated signaling is crucial for the activation of cerebral blood vessel endothelial cells and microglia in murine Toxoplasma encephalitis . ^^^ The regulatory role of interferon gamma receptor ( IFN gammaR ) and tumor necrosis factor receptor ( TNFR ) mediated immune reactions for the activation of cerebral endothelial cells , microglia , and astrocytes was evaluated in a model of murine Toxoplasma encephalitis ( TE ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Studies in mice have delineated an important role for CD 4 ( + ) T cells and cytokines including interferon gamma and tumor necrosis factor alpha in the response to infection with mycobacteria . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
BACKGROUND : Severe systemic toxicity and hemodynamic changes after isolated limb perfusion ( ILP ) with tumor necrosis factor alpha ( TNF alpha ) and melphalan , with or without interferon gamma , have been reported in several series . ^^^ METHODS : Clinical and pharmacokinetic data for 20 consecutive patients with recurrent melanoma of the limbs who were treated by ILP with TNF alpha ( 3 4 mg ) and melphalan , with or without interferon gamma , were studied . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To investigate the effects of this therapy in the central nervous system , we serially analyzed from days 7 to 17 postinoculation the gene expression pattern of tumor necrosis factor ( TNF ) , lymphotoxin , interferon gamma , interleukin 1beta , the inducible nitric oxide synthase ( iNOs ) , interleukin 10 , the vascular cell adhesion molecule 1 ( VCAM 1 ) and the intercellular adhesion molecule 1 ( ICAM 1 ) in the spinal cord of Lewis rats with actively induced EAE , treated with Rolipram . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Apoptosis in response to TNFalpha was observed in NIT 1 cells whereas apoptosis of primary beta cells required both TNFalpha and interferon gamma ( IFNgamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Peripheral blood mononuclear cells from healthy persons without HIV 1 infection had increased proliferative and cytokine ( interleukin 4 , interferon gamma , and tumor necrosis factor [ TNF ] alpha ) responses to stimulation with the crude Cryptosporidium whole antigen . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , synergy between rIFN gamma and TO was mainly dependent on TO induced tumor necrosis factor alpha ( TNF alpha ) secretion . ^^^ These results suggest that the capacity of TO to increase NO production from rIFN gamma primed mouse peritoneal macrophages is the result of TO induced TNF alpha secretion . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
DRalphabeta1 * 0401 restricted , antigen specific T cells produced significantly more interferon gamma and tumor necrosis factor a in response to HCgp 39 than did T cells from DRalphabeta1 * 0402 transgenic mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After tumor necrosis factor alpha and interferon gamma stimulation , five cell lines exhibited distinct ICAM 1 induction . ^^^ These results suggest that ICAM 1 is overexpressed and inducible by tumor necrosis factor alpha and interferon gamma in pancreatic cancer cell lines . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , electrophoretic mobility shift analyses along with the employment of transcription factor decoy oligodeoxynucleotides demonstrated that tumor necrosis factor alpha via nuclear kappaB and interferon gamma via signal transducer and activator of transcription 1 up regulate CD 40 gene expression in rat aortic cultured smooth muscle cells . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Of the cytokines reported to enhance neutrophil survival , transcripts for interleukin ( IL ) 1alpha , IL 1beta , IL 6 , and granulocyte macrophage colony stimulating factor ( GM CSF ) ( but not interferon gamma or granulocyte colony stimulating factor [ G CSF ] ) were detected by ribonuclease protection assay in basal and tumor necrosis factor ( TNF ) alpha stimulated epithelial cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In these pure lymphocyte preparations the constitutive expression of interleukin 1 ( IL 1 ) , IL 2 , IL 10 , interferon gamma ( IFNgamma ) , and tumor necrosis factor alpha ( TNFalpha ) was determined by using a polymerase chain reaction assisted mRNA amplification assay . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma , IL 2 , IL 4 , IL 10 and TNFalpha secretions in multiple sclerosis patients treated with an anti CD 4 monoclonal antibody . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma significantly increased TNF alpha release and cotinine significantly increased NO release . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TRAIL message was expressed in intrathyroidal lymphocytes isolated from a patient with thyroiditis , and unexpectedly , thyroid follicular cells themselves could be induced to express abundant TRAIL message in the presence of the inflammatory cytokines interferon gamma , tumor necrosis factor alpha , and interleukin 1beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Regulation of MIF expression was investigated by treatment of cultured fibroblast like synoviocytes ( FLS ) with interleukin 1beta ( IL 1beta ) , TNFalpha , or interferon gamma ( IFNgamma ) , and dexamethasone ( DEX ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Supernatants were removed after 48 or 96 h and analysed for T helper 1 type cytokines interferon gamma , TNFalpha and T helper 2 type cytokines IL 4 , IL 10 by sandwich ELISA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cla 1 expression is suppressed by the proinflammatory stimuli lipopolysaccharide , interferon gamma , and tumor necrosis factor alpha in monocytes and macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma alone did not induce the release of HMG 1 , but it enhanced TNF induced HMG 1 release . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : There were no significant differences in TNFalpha production in the 3 h ( P = 0 . 28 ) or 24 h cultures ( P = 0 . 18 ) or following stimulation with interferon gamma ( P = 0 . 17 ) between the group positive for the conserved haplotype and the group negative for this haplotype . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha and interferon gamma ( IFNgamma ) have been implicated in the pathogenesis of autoimmune diseases , and both cytokines are targets for therapeutic intervention . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Recipients of pZP 3 specific T cells developed severe granulomatous oophoritis , and the diseased ovaries had elevated ovarian mRNA levels of interferon gamma , interleukin 1beta , and tumor necrosis factor alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , in the presence of tumor necrosis factor alpha ( TNFalpha ) and / or interferon gamma ( IFNgamma ) , HIEC became highly susceptible to FAS induced apoptosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using cultured mouse tubular epithelial cells , the regulation of the HA production in vitro in response to tumor necrosis factor alpha and interferon gamma was also examined . ^^^ Analyzing a kidney tubular epithelial cell line in vitro , it was found that tumor necrosis factor alpha and interferon gamma , and particularly the combination of these two cytokines , markedly enhanced the synthesis of HA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , synergy between rIFN gamma and SB was mainly dependent on SB induced tumor necrosis factor alpha ( TNF alpha ) secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We also observed the systemic antitumor immune response in the tumor bearing mice and demonstrated that NK and CTL activity of splenocytes and the production of IL 2 , tumor necrosis factor and interferon gamma from splenocytes increased obviously in mice after the combined transfer of IL 2 and IL 6 gene . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mouse peritoneal macrophages secrete high levels of TNF alpha after incubation with rIFN gamma plus SSAE . ^^^ These results show that the capacity of SSAE to increase NO production from rIFN gamma primed mouse peritoneal macrophages is the result of SSAE induced TNF alpha secretion . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNFalpha ) and interferon gamma ( IFNgamma ) each increased DAF expression but not CD 59 or MCP expression , and a combination of these cytokines was more potent than either alone . ^^^ Tumor necrosis factor alpha ( TNFalpha ) and interferon gamma ( IFNgamma ) each increased DAF expression but not CD 59 or MCP expression , and a combination of these cytokines was more potent than either alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
On multivariate analysis , hyperparasitemia , jaundice , and shock were all associated independently with raised IL 6 , IL 10 , and interferon gamma , and acute renal failure specifically with raised TNF alpha levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Melanocortin peptides significantly inhibited release of both NO ( ) ( 2 ) and TNFalpha into cell free supernatants from microglia stimulated with Abeta [ 1 42 ] or Abeta [ 25 35 ] peptides and interferon gamma ( IFNgamma ) . ^^^ Alpha MSH peptides inhibit production of nitric oxide and tumor necrosis factor alpha by microglial cells activated with beta amyloid and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , the effect of only phorbol ester , opsonized zymozan , peptide fMLP , and platelet activating factor led to ROS generation , whereas tumor necrosis factor alpha , interferon gamma , and lipopolysaccharide did not stimulate macrophage oxidative burst . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Injection ( intravenous ) of ODNs formulated in LPD into mice triggered production of proinflammatory cytokines including interferon gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Integrin expression was assessed by qualitative methods ( immunocytochemistry , Western blotting ) and semi quantitative techniques ( FACS , cellular ELISA ) , before and after stimulation by TNFalpha , IL 1 beta , TGF beta , FGF 2 , interferon gamma and interferon alpha 2b . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our data show that photodynamic treatment of highly pure murine bone marrow derived macrophages with the hematoporphyrin derivative Photosan 3 does not result in activation of these cells for cytotoxicity against YAC 1 tumor cells or secretion of tumor necrosis factor and nitric oxide , irrespective of co stimulation with interferon gamma , a potent priming agent for macrophage antitumoral activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , the IL 6 and IL 8 levels were significantly decreased ( p < 0 . 05 ) after treatment , while tumor necrosis factor ( TNF ) alpha and interferon gamma remained almost normal . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Secretion of tumor necrosis factor alpha ( TNFalpha ) , interferon gamma , interleukin 10 ( IL 10 ) , and IL 4 by peripheral blood mononuclear cells ( PBMC ) from 53 patients with early ReA ( disease duration < 8 weeks , 64 % HLA B 27 positive ) and 30 patients with early , untreated RA ( disease duration < 6 months ) was determined by enzyme linked immunosorbent assay ( ELISA ) after ex vivo stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukins , interferon gamma and tumor necrosis factor did not show any changes after surgery . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : The production of interleukin 2 ( IL 2 ) , interferon gamma , IL 10 , IL 12 and tumor necrosis factor alpha was found to be significantly lower in the patients ( 75 + / 57 , 171 + / 205 , 40 + / 34 , 8 + / 8 , 1450 + / 1010 pg / ml ) than in healthy subjects ( 143 + / 99 , 422 + / 296 , 64 + / 34 , 16 + / 10 , 2550 + / 950 pg / ml ) ; however , the mean level of IL 4 in the patients seemed to be higher . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To investigate cytokine regulation in cells of freshly excised lymphoid tissues , rigorous quantitative reverse transcription / polymerase chain reaction ( QRT PCR ) assays were developed to measure attomole ( 10 ( 18 ) mol ) amounts of the mRNA for seven cytokines : interleukin 1alpha ( IL 1alpha ) , IL 1beta , tumor necrosis factor alpha ( TNFalpha ) , interferon gamma ( IFNgamma ) , transforming growth factor beta ( TGFbeta ) , IL 2 and IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TRSB and TR 339 induced high serum levels of alpha / beta interferon , gamma interferon , tumor necrosis factor alpha , interleukin 6 , and corticosterone and induced pathology reminiscent of lipopolysaccharide induced endotoxic shock , a type of systemic inflammatory response syndrome . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Profiles of the cytokine mRNA expression for interleukin ( IL ) 4 , interleukin ( IL ) 6 , interleukin ( IL ) 10 , interleukin ( IL ) 12 , tumour necrosis factor ( TNF ) alpha , tumour necrosis factor ( TNF ) beta , transforming growth factor ( TGF ) beta and interferon ( IFN ) gamma were examined at 4 h , 8 h and 12 h postinfection ( p . i . ) , and in noninfected controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
BEAS 2B constitutively expressed IL 16 messenger RNA ( mRNA ) and protein ; IL 16 expression was significantly upregulated in a concentration dependent manner within 24 h by stimulation with histamine , IL 1beta , or tumor necrosis factor ( TNF ) alpha whereas interferon gamma did not significantly increase IL 16 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
For this purpose , confluent fibroblast cultures ( derived from lung tissue from a nonasthmatic donor ) were stimulated for 4 h with interleukin ( IL ) 1beta , tumour necrosis factor ( TNF ) alpha , interferon ( IFN ) gamma , IL 4 , IL 5 or transforming growth factor ( TGF ) beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
By 8 hours of reperfusion there were large increases in serum levels of interferon gamma ( IFNgamma ) and tumor necrosis factor alpha ( TNFalpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , in contrast to results of recent in vitro studies , in the BB / W rat Fas / FasL expression is not regulated by IL 2 , 4 , 6 , 10 , 12 , interferon gamma , and tumor necrosis factor alpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Type 1 T helper ( Th 1 ) cells produce interferon gamma , interleukin ( IL ) 2 , and tumour necrosis factor ( TNF ) beta , which activate macrophages and are responsible for cell mediated immunity and phagocyte dependent protective responses . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Relative to controls , NOD mice pretreated with rhIL 11 also showed significantly diminished blood levels of TNF , interferon gamma , and IL 12 induced by anti CD 3 antibody and / or lipopolysaccharide . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Macrophages incubated with chicken cystatin alone or with interferon gamma plus chicken cystatin produced increased amounts of both tumor necrosis factor alpha and interleukin 10 . ^^^ The addition of neutralizing anti ( tumor necrosis factor alpha ) antibodies reduced sharply NO production by chicken cystatin / interferon gamma activated mouse peritoneal macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This open , multicentre , randomized phase 2 trial was conducted to determine the effect of isolated limb perfusion ( ILP ) with tumour necrosis factor alpha ( TNFalpha ) in combination with melphalan with or without interferon gamma ( IFNgamma ) in patients with in transit metastases of melanoma of the limbs ( MD Anderson stage IIIA or IIIAB , AJCC stage 3 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Effects of the cytokines tumor necrosis factor ( TNF ) alpha and interferon gamma ( IFN ) were studied because of previous demonstrations of their role in Fas mediated apoptosis in other cell types . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lipopolysaccarides and cytokines , like tumor necrosis factor , interleukin 1 and interferon gamma , have been shown to induce iNOS in the endothelium , vascular smooth muscle cells , macrophages and different parenchymal cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , synergy between rIFN gamma and SL was mainly dependent on SL induced tumor necrosis factor alpha ( TNF alpha ) secretion . ^^^ The present results indicate that the capacity of SL to increase NO production from rIFN gamma primed mouse peritoneal macrophages is the result of SL induced TNF alpha secretion via the signal transduction pathway of PKC activation . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found as expected that clearance from transient infections occurred subsequent to the appearance of CD 4 ( + ) and CD 8 ( + ) T cells and the production of interferon gamma and tumor necrosis factor alpha in the infected liver . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Monomers and dimers repressed NO production , TNF alpha secretion and NF kappaB dependent gene expression induced by interferon gamma , whereas the trimeric procyanidin C 2 and Pycnogenol enhanced these parameters . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In Crohn ' s disease tissue there is considerable evidence for an ongoing T helper cell type 1 response , with excess interleukin 12 , interferon gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Levels of antidiuretic hormone , plasma renin activity , aldosterone , interferon gamma , interleukin 1 , interleukin 6 , tumor necrosis factor alpha , endotoxin , and nitrates nitrites were determined . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : HCV positive liver tissues ( chronic hepatitis , n=10 ; cirrhosis , n=5 ) were immunostained for CD 68 , MAC 387 , and semiquantitated by polymerase chain reaction for intrahepatic cytokine mRNAs ( interferon gamma ( IFNgamma ) , interleukin 1beta ( IL 1beta ) , IL 6 , IL 18 , tumour necrosis factor alpha ( TNFalpha ) , and macrophage inflammatory protein 1beta ( MIP1beta ) ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the alterations in mRNA expression of tumor necrosis factor alpha ( TNFalpha ) , interleukin 1beta ( IL 1beta ) , and interferon gamma ( IFNgamma ) , cytokines related to neuronal damage , in cerebrum and peripheral immune cells of mice after exposure to aluminum through drinking water . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS AND RESULTS : When cultured murine mesangial cells were stimulated with interferon gamma ( IFgamma ) and tumor necrosis factor alpha ( TNFalpha ) , mRNA levels for ICAM 1 and VCAM markedly increased . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma consistently activated KSHV replication , whereas tumor necrosis factor , interleukin 1 , interleukin 2 , interleukin 6 , granulocyte macrophage colony stimulating factor , and basic fibroblast growth factor did not . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Plasma IL 6 , IL 1beta , and TNF alpha levels were not different between rhIL 11 treated animals and the control group ; however , interferon gamma levels were significantly reduced by IL 11 treatment ( 2477 vs . 0 pg / mL , P < . 01 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Rat interleukin ( IL ) 1alpha , 1beta1 , 2 , 6 , interferon gamma , and tumor necrosis factor alpha ( TNF alpha ) genes were also expressed after the early days of transplantation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We also found that costimulation with anti CD3 / CD40L resulted in enhanced production of interleukin ( IL ) 10 , interferon gamma , and tumor necrosis factor alpha but not IL 2 or IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We propose that the proinflammatory cytokines tumor necrosis factor alpha ( TNFalpha ) and interferon gamma ( IFNgamma ) contribute to the WA 1 associated disease . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
ICE messenger RNA ( mRNA ) expression was highly up regulated after 6 , 24 , and 72 h exposure of human islets to interferon ( IFN ) gamma , tumor necrosis factor ( TNF ) alpha + IFNgamma or IL 1beta + TNFalpha + IFNgamma , paralleled by increased iNOS ( the inducible form of NO synthase ) expression and NO production after exposure to the combined cytokines but not to IFNgamma or TNFalpha + IFNgamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
H . pylori is one of the most frequent causes of gastroduodenal infection worldwide , resulting in the release of various bacterial and host dependent cytotoxic substances including ammonia , platelet activating factor ( PAF ) , cytotoxins and lipopolysaccharides ( LPS ) as well as cytokines such as interleukins ( IL ) 1 12 , tumor necrosis factor alpha ( TNF ( alpha ) , interferon gamma ( INFgamma ) and reactive oxygen species ( ROS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Garlic is reported to stimulate immunity , including macrophage activity , natural killer and killer cells , and LAK cells , and to increase the production of IL 2 , TNF , and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It has been suggested that this beneficial effect is paralleled by an inhibition of proinflammatory cytokines such as interferon gamma ( IFNgamma ) and tumor necrosis factor alpha ( TNFalpha ) and an induction of anti inflammatory cytokines such as interleukin 4 ( IL 4 ) and interleukin 10 ( IL 10 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
They bind to specific receptors , CD 95 and TNF R , which are induced either by other cytokines , such as interferon gamma , or by Helicobacter pylori itself . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It has previously been demonstrated that a nonvirulent strain of Salmonella typhimurium mutant ( ST ) can be used not only as a vehicle in oral genetic immunization via the intestinal mucosa , but also as an enhancer of interferon gamma and tumor necrosis factor alpha mediated immunity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cachexia is associated with higher than normal concentrations of tumor necrosis factor alpha ( TNF alpha ) , interleukin ( IL ) 1 , IL 6 , serotonin , and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Additionally , no production of fas ligand , tumor necrosis factor alpha or interferon gamma in LS 801 was observed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , hepatic and serum interleukin ( IL ) 2 , interferon gamma , and TNF alpha were enhanced , without changes in IL 4 and IL 10 . ^^^ Phenotypically , hepatic infiltrates in allografts with HBV recurrence were comprised of CD4+ lymphocytes and macrophages with a correlation between interferon gamma and TNF alpha producing cells and the degree of necroinflammatory activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine gene expression was assessed by RT PCR using paired primers for interferon gamma ( IFNgamma ) , tumor necrosis factor alpha ( TNFalpha ) , interleukin ( IL ) 1beta , IL 2 , IL 4 , IL 6 , IL 10 , CD 4 , CD 8 , and glyceraldehyde 3 phosphate dehydrogenase . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma augmented the activation of CPP 32 by TNF alpha in HOG cells and O2A ( + ) oligodendrocyte precursor cells but had no effect on mature oligodendrocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Anti TNFalpha therapy was also more effective than anti CD 4 in reducing Thl activity , as assessed by the production of interferon gamma in lymph node cell cultures . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This work indicates that incubation of human umbilical cord endothelial cells with combinations of interleukin 1beta , tumor necrosis factor alpha , and interferon gamma stimulated the synthesis of iNOS mRNA , as detected by reverse transcriptase polymerase chain reaction . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Eight patients with RA ( disease duration < six months ) were studied serially before , after three , and after six to nine months of treatment with MTX for the cytokines tumour necrosis factor alpha ( TNFalpha ) , interferon gamma ( IFNgamma ) , interleukin 4 ( IL 4 ) and interleukin 10 ( IL 10 ) by intracellular staining of T cells derived from peripheral blood . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
With the use of neutralizing antibodies against interferon gamma , granulocyte macrophage colony stimulating factor , and tumor necrosis factor ( TNF ) alpha , it was determined that TNF alpha is one of the soluble factors released by NQ 22 cells involved in the up regulation of VCAM 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both preincubation of MKN 45 cells with interferon gamma for 6 h and coculture with TNF alpha significantly increased the cytotoxicity of H . pylori . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When delivered into mice challenged with lipopolysaccharide , cSN 50 potently blocked the production of proinflammatory cytokines ( tumor necrosis factor alpha and interferon gamma ) and significantly reduced the lethality associated with ensuing endotoxic shock . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , this intracellular marker of priming is selective for IL 5 related cytokines , in that it is observed following exposure to IL 5 and granulocyte macrophage colony stimulating factor but not to interferon gamma , stem cell factor , tumor necrosis factor alpha , or IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : We present four methods to enhance the acquisition and analysis of cells secreting the cytokines interferon gamma ( IFNgamma ) , tumor necrosis factor alpha ( TNFalpha ) , interleukin 2 ( IL 2 ) , and interleukin 4 ( IL 4 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The induction of IP 10 appeared to be direct because infection with adenovirus vectors failed to induce the expression of the potent IP 10 stimulators , interferon gamma and tumor necrosis factor alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A comparable pattern was also found in FCS elicited macrophages which appeared not to be responsive to the challenge with interferon gamma plus LPS , as measured by the generation of NO and tumor necrosis factor alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : We examined , in nine normal volunteers , the in vitro effects of moclobemide on the production of interleukin 6 ( IL 6 ) , IL 8 , tumor necrosis factor alpha ( TNFalpha ) , interferon gamma ( IFNgamma ) , IL 10 and the IL 1 receptor antagonist ( IL 1RA ) by diluted whole blood stimulated or not with lipopolysaccharide ( LPS ) +phytohemagglutinin ( PHA ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This is recognized by T lymphocytes ( CD 4+ ) resulting in the release of cytokines ( tumor necrosis factor , interleukin 2 , interferon gamma , and others ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , MCP 1 mRNA and protein were found to be expressed by control isolated peritubular cells , and expression was markedly stimulated by interleukin 1alpha and 1beta ( IL 1alpha and IL 1beta ) , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma , and LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
At variance with rodents , the inducible isoform of the NO synthase was expressed neither in immature human DC nor after cell treatment with TNFalpha , interferon gamma , and lipopolysaccharide , suggesting that regulation of these cells depends on exogenous NO . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of interleukin 1beta , 6 , and 8 , interferon gamma , and tumor necrosis factor alpha were measured by semiquantitative polymerase chain reaction using beta actin as an internal standard . ^^^ The expression of interleukin 1beta , interferon gamma , and tumor necrosis factor alpha , however , increased only half as much in smokers as in nonsmokers . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
VCAM 1 was undetectable before or after culturing but was strongly upregulated on endothelial cells by incubation with the cytokines TNF alpha , IL 1alpha or interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The initial systemic hyperinflammation is caused by production of inflammatory cytokines , especially tumour necrosis factor a ( TNF alpha ) , and also interleukin 1 ( IL 1 ) , IL 6 , and interferon gamma , which act synergistically with TNF alpha in inducing shock in animal models . ^^^ Interferon gamma 1 b has an immunoregulatory effect in patients with immunoparalysis during the compensatory anti inflammatory response syndrome , not only restoring levels of HLA DR expression but also reestablishing the ability of monocytes to secrete cytokines such as TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here we show that 16K PRL , but not full length PRL , acts to promote the expression of the inducible isoform of nitric oxide synthase ( iNOS ) and nitric oxide ( * NO ) production by pulmonary fibroblasts and alveolar type 2 cells with potency comparable with the proinflammatory cytokines interleukin 1beta , interferon gamma , and tumor necrosis factor alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This stimulating effect is strongly potentiated by tumor necrosis factor alpha and interferon gamma and is mediated by a rapid increase of the cytosolic calcium concentration . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We evaluated the spontaneous and mitogen induced tumour necrosis factor alpha ( TNFalpha ) and interferon gamma ( IFNgamma ) production of human milk leucocytes and isolated peripheral blood lymphocytes in vitro with a commercial ELISA kit . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression from the human occludin promoter is affected by tumor necrosis factor alpha and interferon gamma . ^^^ Tumor necrosis factor alpha and another cytokine involved in inflammation , interferon gamma , reduced transepithelial resistance in HT 29 / B6 cells , which was preceded by a decrease in occludin mRNA expression as revealed by northern blot analysis . ^^^ Tumor necrosis factor alpha and interferon gamma diminished occludin promoter activity alone and even synergistically , suggesting a genomic regulation of alterations of the paracellular barrier . ^^^ In conclusion , proinflammatory cytokines such as tumor necrosis factor alpha and interferon gamma can downregulate the expression of the transmembrane tight junction strand protein occludin , paralleling the barrier disturbance detected electrophysiologically . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Neither cytotoxic nor noncytotoxic strains induced apoptosis , but death of MKN 45 cells was induced by pre treatment with interferon gamma and culture with TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of both NB cell lines with interleukin 1alpha ( 0 . 5 ng / ml ) , tumor necrosis factor alpha ( 100 U / ml ) , interferon gamma ( 200 U / ml ) , or their combinations increased monocyte adhesion to SK N SH and SK N MC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the T cell dependent model of concanavalin A induced hepatotoxicity , GSH depletion resulted in a suppression of interferon gamma release , delay of systemic TNF release , hepatic nuclear factor kappaB activation , and an abrogation of sinusoidal endothelial cell detachment as assessed by electron microscopy . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effect of pretreatment of cultures with cytokines interferon gamma ( IFN ) and tumor necrosis factor alpha ( TNF ) was examined because these cytokines demonstrated effects on Fas mediated apoptosis in other cell types . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Murine bone marrow cells were incubated with lipopolysaccharide together with tumor necrosis factor alpha , interferon gamma and interleukin 1 beta for 48 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In whole blood stimulated with heat killed Burkholderia pseudomallei , neutralization of tumor necrosis factor , interleukin 12 , or interleukin 18 inhibited granzyme secretion , which was independent of interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effect of rIFN gamma was enhanced markedly by the addition of recombinant TNF alpha ( rTNF alpha ) in a dose dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Reverse transcriptase polymerase chain reaction was performed to detect TNF alpha , interleukin 2 , interferon gamma , and inducible nitric oxide synthase expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Proliferative responses and the production of interleukin 2 ( IL 2 ) , interferon gamma ( IFNgamma ) , tumor necrosis factor alpha ( TNFalpha ) , IL 6 , IL 4 , and IL 5 were measured under these costimulatory conditions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our recent studies indicate the ability of cytokines , e . g . , tumor necrosis factor alpha ( TNFalpha ) , interleukin 1beta ( IL 1beta ) and interferon gamma ( IFNgamma ) , to induce the inducible nitric oxide ( iNOS ) and secretory phospholipase A 2 ( sPLA 2 ) genes in immortalized astrocytes ( DITNC ) ( Li et al . , J . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The recent identification and purification of other biologically active cytokines , such as tumor necrosis factor and interleukin 2 , and of monoclonal antibodies that recognize unique cell surface antigens on renal carcinoma cells , provide exciting possibilities for combination regimens with various interferon species . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When peripheral blood mononuclear cells ( PBMC ) derived from head and neck cancer patients were treated with cisplatin or with 5 FU , interferon gamma , tumor necrosis factor ( TNF ) alpha , TNF beta , interleukin ( IL ) 1beta , IL 6 , IL 12 and IL 18 as well as killer cell activities were significantly induced in both groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neither tumor necrosis factor alpha ( TNF ) , interleukin 1 alpha ( IL 1 ) , nor interferon gamma ( INF ) altered the growth of CPAECs treated by single radiation doses . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To evaluate the effect of exogenous nitric oxide ( NO ) and endogenous NO on the production of prostacyclin ( PGI ( 2 ) ) by cultured human pulmonary artery smooth muscle cells ( HPASMC ) treated with lipopolysaccharide ( LPS ) , interleukin 1 ( beta ) ( IL 1 ( beta ) ) , tumor necrosis factor alpha ( TNF ( alpha ) ) or interferon gamma ( IFN ( gamma ) ) , HPASMC were treated with LPS and cytokines together with or without sodium nitroprusside ( SNP ) , NO donor , N ( G ) monomethyl L arginine ( L NMMA ) , NO synthetase inhibitor , and methylene blue ( MeB ) , an inhibitor of the soluble guanylate cyclase . ^^^ However , stimulatory effect of SNP on PGI ( 2 ) production was not found in TNF ( alpha ) and IFN ( gamma ) treated HPASMC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
INTERVENTIONS : Sterile , endotoxin and cytokine free pancreatic ascites tested for interleukin ( IL ) 1beta , TNF alpha , interferon gamma , and IL 6 was obtained from rats 18 hrs after the induction of severe , acute pancreatitis . ^^^ CONCLUSIONS : A component of pancreatic ascites other than endotoxin , bacteria , or cytokines ( IL 1beta , TNF , interferon gamma , or IL 6 ) is capable of inducing ARDS in healthy animals . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In order to further evaluate the relevance of TNF alpha to host resistance in chlamydial genital tract infection , we examined the effect of local inhibition of the TNF alpha response in normal C 57 mice and in interferon gamma gene deficient C 57 mice infected intravaginally with the mouse pneumonitis agent of Chlamydia trachomatis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Autoimmune / inflammatory diseases are associated with excessive production of inflammatory cytokines such as IL 1 , IL 12 , tumor necrosis factor alpha ( TNFalpha ) and interferon gamma ( IFNgamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We measured production of tumor necrosis factor ( TNF ) alpha , interferon gamma , interleukin ( IL ) 10 , and IL 4 by splenic CD4+ , CD8+ , and gammadelta+ T cells using flow cytometry . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Concentrations of the T helper ( Th ) 1 cytokines interleukin ( IL ) 2 , tumour necrosis factor ( TNF ) alpha , TNF beta and interferon gamma , Th 2 cytokines IL 4 , IL 5 , IL 6 , IL 10 as well as those of soluble CD 30 in sera have been examined during the three trimesters of gestation , at delivery in normal pregnancy , and at the time of spontaneous abortion in women with a history of unexplained recurrent spontaneous abortion ( RSA ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Recombinant porcine interferon gamma weakly stimulated ICAM 1 expression when incubated alone with PAEC but had an inhibitory effect on the increase in ICAM 1 due to TNF alpha , both at 8 and 24 hr . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Transient expression of STAT 6 in a STAT 6 deficient cell line ( HEK 293 ) conferred sensitivity to IL 4 for STAT 6 dependent transcription and for repression of interferon gamma ( IFNgamma ) / STAT1 and / or tumor necrosis factor alpha ( TNFalpha ) / NF kappaB driven reporter gene expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study firstly we investigated the constitutive intracellular expression of Trx , TrxR , the cytokines interleukin ( IL ) 1alpha , IL1beta , IL 2 , IL 4 , IL 6 , IL 8 , IL 10 , tumour necrosis factor alpha ( TNFalpha ) and interferon gamma ( IFNgamma ) in normal melanocytes and ten primary and metastatic malignant melanoma cell lines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , interferon gamma , tumor necrosis factor alpha , and transforming growth factor B ( 1 ) , ( TGF beta ( 1 ) ) are powerful inhibitors of erythropoiesis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Bioparox Spray also has an independent anti inflammatory effect by blocking the inflammation mediators : Bioparox Spray inhibits the synthesis of free radicals and the action of IL 1 and TNF as pro inflammatory factors , and it potentiates the action of IL 2 and interferon gamma which are anti inflammatory factors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have investigated the effects of tumor necrosis factor alpha ( TNF alpha ) and interferon ( INF gamma ) , the potent Bacillus Calmette Guerin ( BCG ) induced cytokines on the production of MMP 2 , MMP 9 , TIMP 1 , TIMP 2 and MT 1 MMP in high grade human bladder cancer cell lines , T 24 , J 82 and HT 1376 cell lines . ^^^ However , J 82 cells treated with TNF alpha and INF gamma revealed dose dependent increases of MMP 9 expression and activity with similar baseline expression and activity of MMP 2 . ^^^ These results indicate that TNF alpha and INF gamma could regulate the production of MMP 2 or MMP 9 on bladder cancer cells and their patterns of regulation are cell specific . ^^^ Furthermore , this diverse response of bladder cancer cells to TNF alpha and INF gamma suggests that BCG immunotherapy may enhance the invasiveness of bladder cancer in certain conditions with induction of MMPs . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It is generally believed that atopic dermatitis is a Th 2 type disease , i . e . , the T lymphocytes produce interleukin 4 , interleukin 5 , interleukin 10 , and interleukin 13 , although it has become evident in recent years that the cytokine profile in the skin changes during the course of the disease towards a Th 1 Th2 mixed cytokine profile ( interferon gamma , tumor necrosis factor alpha , and interleukin 2 ) . ^^^ To gain insight in the stimulatory mechanisms for TARC production in keratinocytes , as previously observed in mice , we cultured HaCaT cells and found that interferon gamma and tumor necrosis factor alpha work synergistically to induce TARC production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine analysis after antigen stimulation of the seven best proliferating T cell clones ( four specific for amoxicillin and three for ceftriaxone ) revealed that these cells secrete high amounts of interleukin 5 and mostly lower or no amounts of tumor necrosis factor alpha , interleukin 4 , and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
GH had no effect on cell proliferation or the production of tumor necrosis factor alpha ( TNFalpha ) , interleukin 6 ( IL 6 ) , or interferon gamma ( IFNgamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro studies have demonstrated that Mycobacterium leprae per se is a poor stimulus for TNF alpha production by purified monocytes obtained from normal subjects , although this could be enhanced by either exogenous interferon gamma or cell contact with fixed activated T lymphocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here we show that GTP cyclohydrolase 1 mRNA was present in unstimulated hepatocytes and was up regulated 2 to 3 fold concurrently with iNOS induction induced in vivo by LPS injection and in vitro by stimulation with LPS and inflammatory cytokines tumor necrosis factor alpha , interleukin 1 beta , and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Secreted granulocyte monocyte colony stimulating factor , interferon gamma and tumor necrosis factor alpha were measured in TDAC conditioned medium . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To investigate the possible relationship between cytokine induced expression of iNOS and epithelial ion channel function , we measured whole cell current in A 549 cells treated with a mixture of cytokines : tumor necrosis factor , interleukin 1 beta , and interferon gamma for 12 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Importantly , lower levels of interleukin 2 ( IL 2 ) and interferon gamma ( INF gamma ) but not of IL 4 were observed in the lamina propria lymphocyte RNA isolated from the TNF inhibitor recipients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
KB R 7785 inhibited soluble TNF alpha production in spleen cell cultures stimulated by heat killed L . monocytogenes and the drug treatment reduced serum TNF alpha levels in infected mice , whereas the compound was ineffective on the modulation of interferon gamma and interleukin 10 production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin 18 ( IL 18 ) is not only a co stimulus for the induction of interferon gamma but also has direct proinflammatory effects by inducing tumor necrosis factor alpha ( TNF alpha ) , IL 1 , IL 8 and IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human recombinant TNF alpha ( 25 ng / mL ) added during the activation culture resulted in a two fold increase in interferon gamma release ( type 1 response ) and a significant reduction of interleukin 10 ( type 2 response ) after tumor antigen stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study we demonstrate that the pro inflammatory / hemopoietic cytokines , IL 1beta , IL 6 , TNF alpha , and interferon gamma , significantly inhibit gene expression and secretion of leptin by bone marrow adipocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Part of the effect of T helper ( Th ) 2 type cytokines ( IL 4 , IL 5 ) on eosinophils could be mediated by eotaxin , which is a highly specific chemotactic protein induced by various cytokines [ IL 4 , IL 13 , tumour necrosis factor ( TNF ) alpha and interferon gamma ] . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When neuronal PC 12 cells differentiated with nerve growth factor were treated with interferon gamma ( IFNgamma ) and tumor necrosis factor alpha ( TNFalpha ) , iNOS and AS mRNAs and proteins were markedly induced , with AL mRNA and protein being weakly induced . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OBJECTIVE : To compare the prognostic significance of bactericidal / permeability increasing protein ( BPI ) , group 2 phospholipase A 2 ( PLA 2 2 ) , C reactive protein ( CRP ) , tumour necrosis factor alpha ( TNF ) , interleukin 8 ( IL 8 ) and interferon gamma ( IFN ) in terms of predicting severity of sepsis and outcome . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , synergy between rIFN gamma and WSC was mainly dependent on WSC induced tumor necrosis factor alpha ( TNF alpha ) and nuclear factor kappaB ( NF kappaB ) activation . ^^^ The present results indicate that the capacity of WSC to increase NO production from rIFN gamma primed RAW 264 . 7 macrophages is the result of WSC induced TNF alpha secretion via the signal transduction pathway of NF kappaB activation . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Death associated protein kinase ( DAP Kinase ) is a serine / threonine kinase with a death domain that is involved in apoptosis induced by interferon gamma , TNF alpha , and Fas ligand . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast to TNFalpha , interferon gamma ( IFNgamma ) did not significantly affect taurine uptake . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Over prolonged incubation periods , PLC at sublytic concentrations causes EC to undergo profound shape changes similar to those described following prolonged TNF or interferon gamma exposure . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found that T cruzi infected murine embryonic cardiomyocytes produced nitrite and expressed mRNAs for the chemokines chemokine growth related oncogene , monokine induced by interferon gamma , macrophage inflammatory protein 2 , interferon gamma inducible protein , RANTES , and monocyte chemotactic protein , for iNOS , and for the cytokines tumor necrosis factor ( TNF ) alpha and interleukin ( IL ) 1beta . ^^^ Separate addition of IL 1beta , interferon gamma , TNF alpha or monocyte chemotactic protein , macrophage inflammatory protein 2 , and interferon gamma inducible protein , to cultured cardiomyocytes resulted in NO production but low trypanocidal activity . ^^^ However , simultaneous addition of IL 1beta , interferon gamma , and TNF alpha or the chemokines to cultures resulted in the induction of iNOS , high levels of nitrite , and a marked trypanocidal activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Upon in vitro stimulation with PDB / ionomycin , they showed predominantly interferon gamma and interleukin ( IL ) 4 but also tumor necrosis factor alpha and IL 10 production and did not specifically lyse autologous T cell blasts , B cell lines , or other autologous or allogeneic target or CD1d transfected cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Costimulatory effects of interferon gamma and interleukin 1beta or tumor necrosis factor alpha on the synthesis of Abeta 1 40 and Abeta 1 42 by human astrocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OBJECTIVE : To test the hypothesis that ankylosing spondylitis ( AS ) is a T helper cell type 2 polarised disease by quantifying the T cell cytokines interferon gamma ( IFNgamma ) , interleukin 4 ( IL 4 ) , tumour necrosis factor alpha ( TNFalpha ) , and IL 10 at the single cell level in patients with AS in comparison with healthy HLA B 27 negative and HLA B 27 positive controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Previous studies have shown that the pro inflammatory cytokines tumor necrosis factor alpha and interferon gamma reduce the expression of the cystic fibrosis transmembrane conductance regulator ( CFTR ) gene ( CFTR ) in HT 29 and T 84 cells by acting post transcriptionally . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In an effort to understand pathogenic factors that lead to different outcomes in dermatoborrelioses , skin biopsy samples from 42 patients with erythema migrans and 27 patients with acrodermatitis chronica atrophicans were analyzed for mRNA expression of five pro inflammatory cytokines ( tumor necrosis factor alpha , interleukin 1 beta , interleukin 6 , interferon gamma , and interleukin 2 ) and two anti inflammatory cytokines ( interleukin 4 and interleukin 10 ) by in situ hybridization with cytokine specific riboprobes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Radiation of the esophagus of C3H / HeNsd mice with 35 or 37 Gy of 6 MV 10 rays induces significantly increased RNA transcription for interleukin 1 ( Il 1 ) , tumor necrosis factor alpha ( Tnf ) , interferon gamma inducing factor ( Ifngr ) , and interferon gamma ( Ifng ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The concentrations of interleukin 6 ( IL 6 ) , IL 10 , IL 12 , tumour necrosis factor alpha ( TNFalpha ) and interferon gamma ( IFNgamma ) in sera , as well as the production of IL 2 , IL 4 , IL 10 and IFNgamma in mitogen induced peripheral blood mononuclear cells , were assessed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
AIMS / HYPOTHESIS : Interleukin 1 beta ( IL 1beta ) in synergy with tumour necrosis factor alpha ( TNFalpha ) and interferon gamma ( IFNgamma ) is cytotoxic to pancreatic beta cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We studied the effects of the ECM and its components in a `` naive ' ' form or following binding of the inflammatory cytokines interferon gamma ( IFNgamma ) and tumor necrosis factor alpha ( TNFalpha ) on the surface expression of human leukocyte antigen ( HLA ) class 1 , HLA class 2 ( HLA DR ) , and intracellular adhesion molecule 1 ( ICAM 1 ) , on nonmalignant and malignant thyroid cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Serum levels of tumor necrosis factor ( TNF ) alpha , soluble TNF receptor 1 , 2 , interferon ( IFN ) gamma and interleukin ( IL ) 4 were measured in IPH patients , fatty liver patients , chronic hepatitis patients and control subjects . ^^^ RESULTS : Serum levels of TNF alpha , soluble TNF receptor 1 , interferon gamma and IL 4 of IPH patients were not increased in comparison with control subjects . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : IL 4 and IL 10 suppressed the ex vivo activation state of interferon gamma and tumor necrosis factor alpha activated human neutrophils . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
T cells were stimulated in whole blood or whole SF with antigen plus anti CD 28 for 6 hours , brefeldin A was added after 2 hours , and cells were fixed and stained with antibodies against the surface markers CD 4 and CD 69 and against the cytokines interferon gamma ( IFNgamma ) , tumor necrosis factor alpha , interleukin 10 ( IL 10 ) , and IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After density separation spontaneous production of interferon gamma ( IFNgamma ) , TNFalpha , interleukin 6 ( IL 6 ) , and IL 10 by peripheral blood mononuclear cells ( PBMC ) was detected by ELISPOT . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Initial comparisons indicated that tumor necrosis factor alpha induction of epithelial intercellular adhesion molecule 1 required sequential induction of interleukin ( IL ) 12 ( p 70 ) and interferon gamma , and unexpectedly localized IL 12 production to airway epithelial cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human umbilical vein endothelial cells ( HuECs ) and bovine aortic endothelial cells ( BAECs ) were incubated with the inflammatory cytokines recombinant human interleukin 1beta ( IL 1 ) , tumor necrosis factor alpha ( TNF ) , interferon gamma ( gamma IF ) , and interferon beta ( beta IF ) at increasing concentrations ( 0 . 1 to 1 , 000 U / mL ) , for increasing periods ( 6 to 72 hours ) . ^^^ The degradation and accumulation of 125I LDL were significantly increased ( P < . 02 ) in HuECs preincubated with IL 1 ( 100 U / mL ) compared with control incubations without the cytokine or incubations containing gamma IF , beta IF , or TNF . ^^^ Cholesteryl ester synthesis rates in HuECs preincubated with gamma IF , beta IF , or TNF did not differ significantly from the rates in control incubations . ^^^ There were no significant differences in the rate of degradation or accumulation of radiolabeled Ac LDL in control incubations compared with cultures preincubated with IL 1 , gamma IF , beta IF , or TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Culture supernatants of T cell receptor stimulated PBLs after GC therapy contained lower concentrations of interleukin 2 , interferon gamma , and tumor necrosis factor alpha than those from PBLs taken before pulse therapy . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The present data show that the simultaneous exposure to tumor necrosis factor alpha ( TNFalpha ) and interferon gamma ( IFNgamma ) induces cell death with characteristics of apoptosis in cultured rat oligodendrocytes ; TNFalpha alone was ineffective . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Nuclear factor ( NF ) kappaB is a transcription factor that induces the immunoglobulin kappa chain , cytokines such as interleukin ( IL ) 1 , IL 2 , IL 6 , IL 8 , tumor necrosis factor ( TNF ) alpha and interferon gamma , and cell adhesion proteins . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Examination of cytokine responses revealed a previously undescribed shock like syndrome in lethally infected mice characterized by high levels of tumor necrosis factor alpha and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These changes were accompanied by the upregulation of tumor necrosis factor ( TNF ) alpha , interleukin 1beta ( IL 1beta ) , interleukin 6 ( IL 6 ) and interferon gamma ( IFNgamma ) mRNA expressions in the pancreas , but not by marked alterations of serum amylase , lactic dehydrogenase ( LDH ) and histology . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : MMF treatment decreased tumor necrosis factor alpha , interferon gamma , and interleukin 10 secretion induced by SEB . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Of various inflammatory cytokines , such as tumor necrosis factor alpha ( TNF alpha ) , interleukin 1 alpha ( IL 1alpha ) , and interferon gamma ( IFN gamma ) , we observed that IFN gamma most effectively increased the expression of TP in cultured human monocytic U 937 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
DESIGN : Confluent monolayers of HPMC were exposed to cytokines [ tumor necrosis factor alpha ( TNFalpha ) , interleukin 1 beta ( IL 1beta ) , interferon gamma ( IFNgamma ) ] or LPS , individually or in various double and triple combinations , for 24 72 hours . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFN gamma inhibition of MCMV growth was independent of known mechanisms involving IFN alpha / beta , tumor necrosis factor alpha , inducible nitric oxide synthase , protein kinase RNA activated ( PKR ) , RNaseL , and Mx 1 , and did not involve IFN gamma induced soluble mediators . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
First the induction of nitric oxide by lipopolysaccharide ( LPS ) , tumor necrosis factor alpha ( TNFalpha ) , interferon gamma ( IFNgamma ) , alone or their combination , was studied in C 6 glioma cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Interferon gamma ( IFNgamma ) , IL 6 , TNFalpha , and IL 10 cytokines were significantly modified , whereas IL 1 and IL 8 were not detectable in the blood in any phase of infection . sTNF RI , sTNF RII and sIL 6 were significantly increased in the first 2 weeks of infection , but sTNF R levels were not related to the plasma levels of TNFalpha , whereas sIL 6 was directly related to serum IL 6 concentrations . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Patients with NPLE had high numbers of lymphocytes expressing mRNA for tumour necrosis factor alpha ( TNFalpha ) , interferon gamma , and interleukin 10 in blood . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of either NKG2D ligand by target cells triggers NK cell cytotoxicity and interferon gamma secretion by NK cells , as well as nitric oxide release and tumor necrosis factor alpha transcription by macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Remaining tissue was cryostat sectioned , whereafter quantification of the cytokines interleukin 1beta , interleukin 2 , interleukin 4 , interleukin 5 , interleukin 6 , interleukin 10 , tumor necrosis factor alpha , and interferon gamma was made by immunohistochemistry and digitized image analysis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the molecular mechanism of the synergism between interferon gamma ( IFNgamma ) and tumor necrosis factor alpha ( TNFalpha ) documented in a variety of biological occasions such as tumor cell death and inflammatory responses . ^^^ Interferon gamma ( IFNgamma ) and tumor necrosis factor alpha synergism in ME 180 cervical cancer cell apoptosis and necrosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Exposure to tumor necrosis factor alpha ( TNF alpha ) plus interferon gamma , produced a marked increase in the expression of iNOS and in NO production in the culture medium . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study focused on the production of four inflammatory cytokines , interleukin 1 ( IL 1alpha ) , interleukin 6 ( IL 6 ) , tumor necrosis factor alpha ( TNFalpha ) , and interferon gamma ( IFN gamma ) in primary culture of astrocytes under two different injury models which simulated in vivo mechanical trauma ( scratch injury ) and ischemia . ^^^ A slot blot study of culture media showed that the release of IL 1alpha , IL 6 , TNFalpha , and IFN gamma by astrocytes subsequent to scratch and ischemic injury reached approximately twice the control values . ^^^ Astrocytes produce and release interleukin 1 , interleukin 6 , tumor necrosis factor alpha and interferon gamma following traumatic and metabolic injury . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our data indicate that the presence of the dimyristylglycerol is not an absolute requirement for induction of TNFalpha production but can substitute for the interferon gamma priming . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Thirteen patients developed transient hypoxemia , hypotension , or dyspnea that were associated with significant changes in baseline interleukin 6 , interleukin 8 , tumor necrosis factor alpha , and interferon gamma compared with those not experiencing such reactions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expanded CD 8 ( + ) NKT cells produce T ( H ) 1 type cytokines with high levels of IFN gamma and tumor necrosis factor alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Liver TCRgammadelta ( + ) T cell lines from HCV infected individuals had high levels of non major histocompatibility complex ( MHC ) restricted cytotoxic activity against different targets including primary hepatocytes and produced interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and interleukin 8 ( IL 8 ) following activation by anti CD 3 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : In this study , we compared these tests with the terminal deoxynucleotidyl transferase ( TdT ) mediated dUTP nick end labeling ( TUNEL ) assay and analysis of DNA fragmentation after gel electrophoresis in freshly isolated islets obtained from rats , before and after treatment with interleukin 1 beta , interferon gamma , and tumor necrosis factor a , cytokines known to induce islet cell damage . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated , in a random sample of a German population , the association of polymorphisms in the genes encoding the cytokines interleukin 2 ( IL 2 ) , interleukin 4 receptor ( IL 4R ) , interleukin 6 ( IL 6 ) , interleukin 10 , interferon gamma ( IFNG ) , tumor necrosis factor ( TNF ) and intercellular adhesion molecule 1 ( ICAM 1 ) with ( 1 ) secreted levels of the respective proteins after T cell stimulation and ( 2 ) data on selected diseases obtained from a questionnaire . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In cases where a Th 1 cytokine profile predominates , chiefly , interferon gamma , tumor necrosis factor , or interleukin 12 , these cytokines may directly or indirectly be detrimental to early placental cell differentiation and growth and toxic to embryo development . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inability of TNFalpha / PGE ( 2 ) to induce IL 12p70 was not compensated by interferon gamma ( IFNgamma ) , which strongly enhanced the lipopolysaccharide ( LPS ) induced IL 12p70 production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Maximal expression of NOS 2 after CFA immunization requires the presence of functional type 1 tumor necrosis factor alpha receptor ( TNFR 1 ) and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
ICAM 1 expression was examined by flow cytometry , and expression of mRNA in EC encoding interleukin 1 ( IL 1 ) , IL 6 , IL 8 , tumour necrosis factor alpha ( TNFalpha ) , interferon gamma ( IFNgamma ) , and ICAM 1 was examined by semiquantitative reverse transcriptase polymerase chain reaction . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : NO formation in response to interferon gamma ( IFN gamma ) , interleukin 1beta ( IL 1beta ) , and tumor necrosis factor alpha ( TNF alpha ) was correlated with programmed cell death in the investigated cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
FAT 10 is a ubiquitin like protein that is encoded in the major histocompatibility complex class 1 locus and is synergistically inducible with interferon gamma and tumor necrosis factor alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Infection with cytokine expressing recombinant viruses suggested that T cells used interferon gamma , but not tumor necrosis factor alpha , in clearing virus and that populations of neurons differ in responsiveness to this effector pathway . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , interleukin 8 ( IL 8 ) , tumour necrosis factor alpha ( TNFalpha ) and interferon gamma ( IFNgamma ) were compared with ADA in pleural liquid of patients with inflammatory ( 21 cases ) , malignant ( 28 cases ) and tuberculous ( 21 cases ) disease . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The plasma levels of TNF alpha , interferon gamma , interleukin ( IL ) 2 , IL 4 , IL 10 and soluble TNFR 1 and 2 ( sTNFR 1 and 2 ) were quantified by enzyme linked immunosorbent assays . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Calbindin D ( 28k ) expression resulted in increased cell survival in the presence of the cytotoxic combination of the cytokines IL 1beta ( 30 U / ml ) , TNFalpha ( 10 ( 3 ) U / ml ) , and interferon gamma ( 10 ( 3 ) U / ml ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Finally , we determined the expression of proinflammatory cytokines , including interleukin 1beta , interleukin 8 , interferon gamma , and tumor necrosis factor alpha , and of antiinflammatory cytokines ( interleukin 4 and 10 ) by semiquantitative polymerase chain reaction . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A number of proinflammatory cytokines , such as interleukin 6 , tumor necrosis factor alpha , and interferon gamma , have been proposed as mediators of cancer cachexia ; these data suggest that abnormalities in leptin production / release or in its feedback mechanism play a role in cancer patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : TNFalpha , IL 6 , soluble tumor necrosis factor receptor 1 ( sTNF R 1 ) , interferon gamma ( IFNgamma ) , and IL 2 were measured by the ELISA method in sera from six children with encephalopathy before and during therapy , and in six age matched controls with influenza type A virus infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : The murine macrophage cell lines J 774 A1 were preactivated or not with interferon gamma and were then stimulated by IgG 1 immune complexes ( IC ) , free SOD 1 or SOD 1 IC and superoxide anion , nitric oxide , peroxynitrite , and tumor necrosis factor alpha ( TNF alpha ) production was evaluated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , the influence of MPA ( 10 micromol / l ) on a cellular level is observed by measuring the cell surface expression of adhesion molecules on cytokine stimulated HUVECs , using TNFalpha ( 10 ng / ml ) , interferon gamma ( 100 ng / ml ) , interleukin 1beta ( 10 ng / ml ) and interleukin 8 ( 20 ng / ml ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 7 , IL 7R , IL 1alpha , tumor necrosis factor alpha , and interferon gamma messenger RNA expressions were up regulated in control infected mice , whereas only IL 7 messenger RNA was up regulated in A7R34 treated infected mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We also investigated the functional role of NKG2A and found that coligation of CD 3 and NKG2A by specific monoclonal antibodies results in significant inhibition of interferon gamma and tumor necrosis factor alpha production by stimulated CD 4 ( + ) cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , the lipopolysaccharide ( LPS ) induced release of tumor necrosis factor ( TNF ) alpha was increased , and the secretion of interleukin ( IL ) 10 was reduced by Lektinol at a mistletoe lectin 1 ( ML 1 ) concentration of 0 . 5 to 5 ng / ml , whereas the LPS induced secretion of IL 1 beta , IL 6 , IL 12 , and interferon gamma was not affected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma and TNFalpha synergistically induce the inducible isoform of nitric oxide synthase and elicit severe cytotoxicity in pancreatic beta cells . ^^^ GH inhibited tyrosine phosphorylation and DNA binding of signal transducer and activator of transcription 1 promoted by interferon gamma , whereas nuclear factor kappa B activation by TNFalpha was not affected by GH . ^^^ This action of GH may be sufficient to suppress the synergistic induction of inducible nitric oxide synthase by interferon gamma and TNFalpha , thereby preventing the cytotoxicity to beta cells . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
AIM : Objective evaluation of the effectiveness and tolerance of antibodies to interferon gamma ( TNF gamma ) vs those to tumor necrosis factor alpha ( TNF a ) and placebo in patients with rheumatoid arthritis ( RA ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Proinflammatory cytokines including interferon gamma ( IFNgamma ) , interleukin 6 ( IL 6 ) , and tumor necrosis factor alpha ( TNFalpha ) are implicated in the pathogenesis of acute graft versus host disease ( aGVHD ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Nor did it alter the percentage T cells producing IL 2 , interferon gamma , and percentage of monocytes producing TNFalpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
While in males FB 1 treatment caused increased expression of TNFalpha , interleukin ( IL ) 12 p 40 , interferon gamma ( IFNgamma ) , IL 1beta , IL 6 and IL 10 , females showed an increased expression of IL 6 only , and a downward modulation of IFNgamma , indicating gender differences in cytokine pathways in liver activated by FB 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The influx of T cells , macrophages , and neutrophils was dramatically dampened as was the production of the inflammatory cytokines interferon gamma and tumor necrosis factor alpha and the chemokines monocyte chemotactic protein 1 , macrophage inflammatory protein 1alpha ( MIP 1alpha ) , MIP 1beta , and lymphotactin , normally upregulated in the lung during IPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Eight polymorphisms from five different cytokine genes were studied ( tumor necrosis factor alpha , tumor necrosis factor beta , interleukin ( IL ) 6 , IL 10 , and interferon gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
One of the most important findings of this study was that the mRNA expression of TNF alpha , IL 1 alpha , interferon gamma , IL 10 , IL 18 , macrophage inflammatory protein 1 alpha ( MIP 1 alpha ) , transforming growth factor beta ( TGF beta ) and inducible nitric oxide synthase ( iNOS ) persisted as long as 98 days after the virus infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The CD57+ subsets of the T cells of both healthy individuals and patients with ALD express significantly higher amounts of cytoplasmic tumor necrosis factor alpha ( TNF ) and interferon gamma ( IFN ) after 6 h of stimulation than do the CD 57 subsets . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This local homeostasis is disturbed in IBD , leading to hyperactivation of T helper 1 ( Th 1 ) cells with abundant secretion of interferon gamma and tumor necrosis factor ( TNF ) and production of IgG antibodies against commensal bacteria . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our studies on the regulation of IL 10 secretion in OVCAR 3 revealed that ( 1 ) proinflammatory stimuli IL 1beta and TNF alpha , but not LPS , enhance IL 10 secretion , ( 2 ) IL 6 has no influence on the release of IL 10 , ( 3 ) prostaglandin E 2 influences neither the spontaneous nor the TNF alpha or IL 1beta stimulated IL 10 production and ( 4 ) interferon gamma inhibits IL 10 secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this report we demonstrate that nitric oxide independent killing of co cultured mesangial cells by interferon gamma / lipopolysaccharide activated M ( phi ) is suppressed by binding / ingestion of apoptotic cells and is mediated by tumor necrosis factor ( TNF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , IL 4 , IL 6 , IL 10 , IL 12 , interferon gamma ( IFNgamma ) , tumour necrosis factor alpha ( TNFalpha ) and granulocyte macropage colony stimulating factor ( GM CSF ) were studied . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF alpha appeared to contribute to development of IL 10 hyporesponsiveness , because exposure of normal macrophages to TNF alpha but not interferon gamma reduced macrophage responsiveness to IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Several different interleukins , as well as TNF , interferon gamma , and leukemia inhibitory factor , act as cachectins in animal models . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We previously demonstrated that two cytokines , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( INF gamma ) , inhibited transgene expression from this promoter in skeletal and cardiac myocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study , we evaluated whether Th 2 ( 500 U / ml IL 4 plus 100 U / ml IL 10 ) or Th 3 ( 5 ng / ml TGF 1beta ) cytokines may prevent the derangements induced on isolated human islets by prolonged ( 12 or 72 h ) exposure to combined proinflammatory ( 50 U / ml IL 1beta , 1000 U / ml TNF alpha ) and Th 1 ( 1000 U / ml interferon gamma ) cytokines . ^^^ IL 1beta , interferon gamma , and TNF alpha caused no major change in either islet cell survival or Bcl 2 and Bax mRNA expression after a 12 h incubation ; however , a marked increase in the amount of dead cells , with a major decrease of Bcl 2 mRNA expression , was observed after 72 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Keratinocytes were examined for their susceptibility to apoptosis using cytokines produced during an immunologic response to tumor antigens , i . e . , interferon gamma and / or tumor necrosis factor alpha ( TNF alpha ) . ^^^ Whereas interferon gamma and TNF alpha either alone or in combination did not induce apoptosis in keratinocytes , after infection with the retrovirus to block NF kappaB activation they became susceptible to TNF alpha but not Fas induced apoptosis . ^^^ Moreover , when keratinocytes with repressed NF kappaB activity were simultaneously treated with interferon gamma , there was a synergistic induction of apoptosis by TNF alpha that was dependent on FADD , tumor necrosis factor related apoptosis inducing ligand ( TRAIL ) , and caspase activation . ^^^ The ability of interferon gamma when combined with TNF alpha to mediate keratinocyte apoptosis included induction of TRAIL coupled with diminished capacity of keratinocytes with repressed NF kappaB activity to increase the TRAIL decoy receptor 1 , as well as lower levels of several NF kappaB dependent antiapoptotic proteins accompanied by enhanced caspase 8 levels . ^^^ These results indicate that interferon gamma and TNF alpha synergistically induce keratinocyte apoptosis when concomitant induction of NF kappaB is blocked . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokine genes analyzed were TNF alpha , GM CSF , interferon gamma , IL 2 , IL 4 and IL 5 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of ICAM 1 was significantly increased by interferon gamma ( INF gamma ) or tumour necrosis factor alpha ( TNF alpha ) stimulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , cultured adult rat cardiac myocytes were treated with LPS , interleukin 1beta ( IL 1beta ) , tumour necrosis factor alpha ( TNFalpha ) , interferon gamma ( IFNgamma ) or IL 6 for 24 h . mRNA expression was evaluated for cardiac alpha actin ( cAct ) , skeletal alpha actin ( skAct ) , beta and alpha myosin heavy chain ( MHC ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF ) , interleukin ( IL ) 8 , and interferon gamma ( IFN ) in plasma and PLF were measured . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast to hBD 2 , which is upregulated by microorganisms or tumor necrosis factor alpha ( TNF alpha ) , hBD 3 expression is increased particularly after stimulation by interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This present review concentrates on the recent results investigating the role of certain cytokine gene polymorphisms , including tumor necrosis factor alpha , interferon gamma , interleukin 6 ( IL 6 ) , IL 10 , and IL 1 receptor antagonist , in allogeneic stem cell transplantation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Human DC significantly adhered to PAEC ( 38 40 % ) , and this adhesion was augmented ( > 50 % ) upon treatment with either recombinant swine interferon gamma or recombinant human TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Because interferon gamma ( IFNgamma ) and TNFalpha were frequently elevated in gastric mucosa with Helicobacter pylori infection , effects of IFNgamma and TNFalpha on WNT10B expression in MKN 45 cells were investigated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
WNT 14 mRNA was up regulated by interferon gamma ( IFNgamma ) , but not by tumor necrosis factor alpha ( TNFalpha ) , in MKN 45 cells derived from gastric cancer , while expression of WNT14B mRNA was not affected by IFNgamma and TNFalpha in MKN 45 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of tumor necrosis factor alpha ( TNFalpha ) , interleukin 6 ( IL 6 ) and interferon gamma ( IFNgamma ) were studied on the activity of type 2 , 5 ' deiodinase and on the binding of [ 125I ] T ( 4 ) to proteins in human thyroid cytosolic ( supernatant ) and membrane ( pellet ) fractions . ^^^ Inhibition of type 2 , 5 ' deiodinase by tumor necrosis factor alpha , interleukin 6 and interferon gamma in human thyroid tissue . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Even though exercise did not alter the intracellular cytokines ( TNF alpha and interleukin 2 ) or Fas ligand , it did significantly lower interferon gamma in CD4+ and CD8+ cells ( P < 0 . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Meanwhile , granulocyte colony stimulating factor ( G CSF ) , interferon gamma and tumor necrosis factor ( TNF ) activities in the patient serum and supernate of NB 4 cell culture were detected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Analysis of cytokine levels in colon homogenates showed a significant decrease in TNF alpha , IL 6 , and IL 1beta after rhIL 18BPa treatment but no effect on interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Compound 1 exhibited potent inhibitory effect on tumor necrosis factor alpha ( TNF alpha ) formation in lipopolysaccharide ( LPS ) stimulated RAW 264 . 7 cells while 1 and 4 showed potent inhibitory effects on TNF alpha formation in LPS / IFN gamma ( interferon gamma ) stimulated murine microglial cell lines N9 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , ZnSO 4 cotreatment allowed complete regression of inoculated tumors with TNF and interferon gamma , leading to a significantly better survival ( P = 0 . 0045 ) . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The proinflammatory cytokines , interleukin 1beta ( IL 1beta ) , tumor necrosis factor alpha ( TNFalpha ) , and interferon gamma ( IFNgamma ) , are cytotoxic to pancreatic islet beta cells , possibly by inducing nitric oxide and / or oxygen radical production in the beta cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Concomitant activation of THP 1 cells with interferon gamma and immobilized anti TNFRSF 14 monoclonal antibody resulted in synergistic induction of proatherogenic cytokines , such as TNF alpha and interleukin 8 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The role of TNF alpha may depend upon the presence of interferon gamma early in the infection since when TNF alpha was neutralized in interferon gamma gene knockout mice there was a marked increase in splenic macrophages , NK cells and neutrophils but not a significant increase in colony forming units . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Inflammatory markers were 1 ) soluble adhesion molecules ( intercellular adhesion molecule [ ICAM ] and vascular cell adhesion molecule [ VCAM ] ) , 2 ) C reactive protein ( CRP ) , 3 ) fibrinogen ( Fb ) , 4 ) cytokines ( interferon gamma [ IFNgamma ] , tumor necrosis factor alpha [ TNFalpha ] ) , and 5 ) secretory group IIA phospholipase A 2 ( sPLA 2 IIA ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin 6 , tumor necrosis factor alpha and interferon gamma in patients with anorexia nervosa . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MSCs grew at significantly different rates when incubated in the presence of various recombinant human cytokines , of which interferon gamma , tumor necrosis factor alpha , stem cell factor and insulin like growth factor promoted the proliferation of MSCs dramatically , while others tested had no effects on cell growth . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The corresponding production of IL 12 , IL 18 , interferon gamma , tumor necrosis factor alpha , IL 4 , and IL 13 by lamina propria mononuclear cells in the inflamed intestine was measured . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Major histocompatibility complex antigens , interferon gamma , and tumor necrosis factor alpha ( TNF alpha ) were expressed in endothelial and glial cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of WNT 3 mRNA in a gastric cancer cell line MKN 45 was not changed after treatment with tumor necrosis factor alpha ( TNFalpha ) or interferon gamma ( IFNgamma ) , and that of WNT3A mRNA was undetectable before and after treatment with TNFalpha or IFNgamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After 72 h exposure of human pancreatic islets to a cytotoxic cytokine combination of interleukin 1 beta ( 50 U / ml ) , tumor necrosis factor alpha ( 1 , 000 U / ml ) , and interferon gamma ( 1 , 000 U / ml ) , an increase of cell death vs . control islets was demonstrated by TUNEL and cell death detection ELISA method . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have examined expression of the components of the plasminogen activation system in human astrocytoma U 373 MG cells and found that interleukin 1beta ( IL 1beta ) , tumour necrosis factor alpha TNF alpha ) , interferon gamma ( INF gamma ) and epidermal growth factor ( EGF ) specifically regulate the expression of tissue type plasminogen activator ( t PA ) , urokinase type plasminogen activator ( u PA ) , plasminogen activator inhibitor type 1 ( PAI 1 ) and protease nexin 1 ( PN 1 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CSA also potently inhibited the production of IL 15 and TNFalpha stimulated with interferon gamma , IL 1beta , or lipopolysaccharide . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Role of ICAM 1 in the aggregation and adhesion of human alveolar macrophages in response to TNF alpha and INF gamma . ^^^ The present study shows that ICAM 1 expression is significantly elevated on alveolar macrophages from patients with sarcoidosis in response to tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( INF gamma ) compared with healthy controls . ^^^ Aggregation and adhesion were significantly increased in alveolar macrophages treated with TNF alpha and INF gamma , and significantly inhibited in those pretreated with a monoclonal antibody to ICAM 1 . ^^^ These results suggested that the surface expression of ICAM 1 on alveolar macrophages in response to TNF alpha and INF gamma is important in mediating aggregation and adhesion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma pretreatment did not influence the LPS induced TNF alpha response , but considerably increased the TDCM induced response . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although the expression of hDAF on the human endothelial cells increased with a combination of cytokines , tumor necrosis factor alpha and interferon gamma , no cytokine induced upregulation was seen in the cells of transgenic pigs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After conventional analysis of semen samples obtained from 86 men , levels of seminal cytokines ( interleukin [ IL ] 1alpha , IL 2 , IL 4 , IL 6 , IL 8 , tumor necrosis factor alpha [ TNF alpha ] , interferon gamma , granulocyte colony stimulating factor [ G CSF ] , macrophage CFS [ M CSF ] ) and granulocyte elastase were measured by an enzyme linked immunosorbent assay . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Twelve paediatric patients with aplastic anaemia and two groups of normal control subjects underwent flow cytometric analysis for intracytoplasmic expression of gamma interferon ( gamma IFN ) and tumour necrosis factor alpha ( TNF alpha ) in bone marrow and peripheral blood CD4+ and CD8+ cells . ^^^ Marrow CD4+ and CD8+ cells expressing gamma IFN and TNF alpha were significantly increased in the patients in comparison with normal control subjects ( P from < 0 . 05 to < 0 . 0001 in the different cellular subsets ) . ^^^ Patients ' marrow CD4+ and CD8+ cells containing gamma IFN and TNF alpha were significantly increased when compared with the same cell fractions from paired peripheral blood samples ( P from < 0 . 05 to < 0 . 0001 in the various cellular subsets ) . ^^^ In the supernatant of marrow MNCs , gamma IFN and TNF alpha were detected in four out of eight and five out of eight cases , respectively , whereas neither cytokine was traceable in the control subjects . ^^^ Patients ' peripheral blood CD4+ and CD8+ cells containing gamma IFN and TNF alpha were not significantly increased in comparison with those from normal control subjects . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study , we examined the effect polymorphisms in the X 1 box and the Y box on the cytokine ( interferon gamma ( IFNgamma ) , tumor necrosis factor alpha ( TNFalpha ) and granulocyte macrophage colony stimulating factor ( GM CSF ) ) mediated transcriptional activities of DRB 1 promoters in these , i . e . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
T cell activation was determined by measuring the production of three major cytokines ( tumor necrosis factor , granulocyte / macrophage colony stimulating factor and interferon gamma ) , known to be secreted by most activated TILs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Bovine luteal cells from midcycle CL ( Days 8 12 ) were exposed for 24 h to interferon gamma ( IFN ; 50 ng / ml ) and / or tumor necrosis factor alpha ( TNF ; 50 ng / ml ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , treatment with CTLA4Ig was associated with a significant decrease in the intragraft production of tumor necrosis factor alpha , interleukin 2 , and interferon gamma , as well as slightly increased intragraft expression of interleukin 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MIF was also constitutively expressed at the messenger RNA and protein level by several cultured colon and gastric epithelial cell lines , and its expression in those cells was not up regulated by the proinflammatory cytokines interleukin 1alpha , tumor necrosis factor alpha , or interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
One subset , which was CD 4 ( ) , selectively produced the Th 1 cytokines interferon gamma and tumor necrosis factor alpha , and expressed NKG2d , a marker associated with cytolysis of microbially infected and neoplastic cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
FB 1 caused increased expression of tumor necrosis factor alpha ( TNFalpha ) , interleukin ( IL ) 1alpha , IL 1beta , IL 1 receptor antagonist ( IL 1Ra ) , IL 6 , IL 10 , IL 12 p 40 , IL 18 and interferon gamma ( IFNgamma ) in male liver , with a similar increase in females except for IL 1beta and IL 18 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , treatment of peritoneal macrophages with rIFN gamma plus EA caused a significant increase in tumor necrosis factor alpha ( TNF alpha ) production . ^^^ CONCLUSIONS : EA increases the production of NO and TNF alpha by rIFN gamma primed macrophages and suggest that NF kappaB plays a critical role in mediating these effects of EA . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our previous study showed that maximum induction of interferon gamma ( IFNgamma ) and tumor necrosis factor alpha ( TNFalpha ) was observed at 4 and 8 h , respectively , after an acute po FB ( 1 ) treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Finally , by using these human follicular papilla cell lines , we showed that cytokines such as interleukin 1 beta , tumor necrosis factor alpha , interferon gamma , and interleukin 4 , and growth factors such as epidermal growth factor , basic fibroblast growth factor , and transforming growth factor beta 1 , but not vascular endothelial growth factor , hepatocyte growth factor , keratinocyte growth factor , and insulin like growth factor 1 , significantly downregulated the production of leptin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
T lymphocytes exposed to infected MDDCs are significantly impaired in upregulation of CD 69 and CD 28 , are refractory to activation , and die through contact dependent apoptosis mediated by an as yet unidentified mechanism not requiring Fas , tumor necrosis factor related apoptosis inducing ligand , leukocyte function antigen 1 , intercellular adhesion molecule 1 , tumor necrosis factor alpha , interleukin 10 , alpha interferon , gamma interferon , prostaglandins , or reactive nitrogen intermediates . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Polymorphisms in genes encoding tumour necrosis factor alpha ( TNFalpha ) , interleukin ( IL ) 6 , IL 10 , interferon gamma ( IFNgamma ) , transforming growth factor beta ( TGFbeta ) and thiopurine S methyltransferase ( TPMT ) can have significant effects on an individual ' s risk of rejection , as well as their ability to tolerate immunosuppressive therapy . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , HBD 2 mRNA expression was increased by interferon gamma , interleukin ( IL ) 1beta alone and IL 1beta+tumour necrosis factor ( TNF ) alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation with a cytokine mix ( interferon gamma , interleukin 1 beta , and tumor necrosis factor alpha ) induces NOSII mRNA accumulation , as well as promoter activity in these cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In particular , both types of response are typified by the enhanced reactivity of T helper 1 cells with high levels of interleukin 2 , interferon gamma and tumor necrosis factor alpha and are accompanied often by organ specific and / or systemic damage and the production of IgG . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Their expression is regulated in parallel by inflammatory cytokines TNF alpha and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
An RNase protection assay was used to measure changes in rat bladder cytokine mRNA [ interferon gamma ( IFN ) gamma , interleukin 1alpha / beta ( IL 1alpha / beta ) , IL 2 , IL 3 , IL 4 , IL 5 , IL 6 , IL 10 , and tumor necrosis factor alpha / beta ( TNF alpha / beta ) ] after acute ( 4 h ) , intermediate ( 48 h ) , or chronic ( 10 day ) cystitis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Infection of MDM with HIV 1 SF 162 or IIIB led to increased production of tumour necrosis factor alpha ( TNFalpha ) , interleukin 1 beta , interferon alpha and interferon gamma after cell cell contact with PBMC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These included early ( 1 2 h ) expression of inflammatory mediators ( IL 1alpha , IL 1beta , IL 4 , IL 6 , IL 10 , TNF alpha , interferon alpha , interferon gamma , and MCP 1 ) and leukocyte accumulation in lung tissue ( lung myeloperoxidase activity 18 . 5 + / 7 . 8 U / g tissue , P < 0 . 05 ) , followed by pulmonary edema ( lung water content index 17 . 4 % + / 2 . 5 % , P < 0 . 05 ) and mortality . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both the cytokine interferon gamma and the lysosomotropic drug chloroquine , but not the cytokines interleukin ( IL ) 1 , IL 6 , or tumor necrosis factor alpha ( TNF alpha ) , induced an accumulation of APP in newly formed multivesicular body like organelles . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Western blot analysis and immunohistochemistry revealed that tumour necrosis factor alpha ( TNFalpha ) plus interferon gamma ( IFNgamma ) synergistically induce iNOS gene expression in the endothelium but not in the smooth muscle of these segments while constitutive endothelial NO synthase ( eNOS ) abundance was markedly reduced . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha and interferon gamma , which are elevated in gastric mucosa with Helicobacter pylori infection , did not affect the expression level of GIPC 2 mRNA in MKN 45 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Chlorogenic acid , a plant polyphenol , inhibited SE induced T cell proliferation ( by 98 % ) and production of interleukin 1beta , tumor necrosis factor , interleukin 6 , interferon gamma , monocyte chemotactic protein 1 ( MCP l ) , macrophage inflammatory protein ( MIP ) lalpha , and MIP lbeta by human peripheral blood mononuclear cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Aortic vascular smooth muscle cells were incubated with the proinflammatory cytokines interleukin 1beta , tumor necrosis factor alpha , and interferon gamma or with the nitric oxide donor S nitroso N acetylpenicillamine to investigate the regulation of ET ( A ) receptor gene expression during severe inflammation . ^^^ ET ( A ) receptor gene expression was downregulated in aortic vascular smooth muscle cells by tumor necrosis factor alpha but not by interleukin 1beta , interferon gamma , or nitric oxide . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PTX in vitro could suppress T cell proliferation and inhibit TNF alpha and interferon gamma production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The exact role played by interferon gamma , tumor necrosis factor ( TNF ) alpha , and interleukin 1beta in this pathogenic process is still only partially understood . ^^^ We previously reported that it was susceptible to apoptosis induced by TNF alpha , in combination with interleukin 1beta and interferon gamma . ^^^ The presence of cFLIP prevented the weak TNF alpha induced reduction in cellular insulin content and secretion ; however , it did not prevent the decrease in glucose stimulated insulin secretion induced by the combined cytokines , in agreement with our previous data demonstrating that interferon gamma alone could induce these beta cell dysfunctions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor ( TNF ) + interferon gamma ( IFNgamma ) was more potent at inducing cell death in SOCS 1 / islets than in wild type . ^^^ Tumor necrosis factor ( TNF ) + interferon gamma ( IFNgamma ) was more potent at inducing cell death in SOCS 1 / islets than in wild type . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Primary rat hepatocytes were exposed to a cytokine mixture of tumor necrosis factor alpha , interleukin 1beta , interferon gamma and lipopolysaccharide . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The polymorphisms analysed were those of tumour necrosis factor alpha ( TNFA ) , interleukin 6 ( IL 6 ) , interleukin 10 ( IL 10 ) and interferon gamma ( IFNG ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We show that HS can be applied successfully in an antitumor protocol based on TNF and interferon gamma , leading to a significant inhibition of lethality but not to a reduction of antitumoral capacity . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Antigenic stimulation usually follows two pathways : Th 1 , which activates macrophages and cytotoxic T lymphocytes and produces interleukin ( IL ) 2 , IL 3 , interferon gamma , and tumor necrosis factor alpha , and Th 2 , which activates the humoral immune system and produces IL 4 , IL 5 , and IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have previously established that insulin like growth factor ( IGF ) 1 , 2 and insulin exert a strong protective effect against tumor necrosis factor alpha ( TNF ) induced apoptosis in interferon gamma ( IFN ) sensitized HT 29 D4 human colon carcinoma cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , no increments in tumor necrosis factor alpha ( TNF alpha ) and interferon gamma were detected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 8 , MCP 1 and RANTES are regulated by local growth factors and cytokines such as tumor necrosis factor alpha ( TNF alpha ) , interferon gamma , and IL 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHOD OF STUDY : Concentration of interferon gamma ( IFNgamma ) , interferon alpha ( IFNalpha ) , tumour necrosis factor alpha ( TNFalpha ) , interleukin 1beta ( IL 1beta ) and epidermal growth factor ( EGF ) in supernatants ( SNs ) of 24 hr cultures of MNCs obtained from eutopic and ectopic endometrium of women with adenomyosis was determined by enzyme linked immunosorbent assay . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study used semi quantitative reverse transcriptase polymerase chain reactions to determine the expression of gene transcripts for immunosuppressive cytokines ( transforming growth factor beta [ TGFbeta ] and interleukin [ IL ] 10 ) , Th 2 type cytokines ( IL 4 and IL 6 ) and Th 1 type cytokines ( interferon gamma [ IFNgamma ] , tumour necrosis factor alpha [ TNFalpha ] , IL 2 and IL 12 ) in lesional atopic , non lesional atopic and healthy canine skin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When in transit metastases are recurrent , deep seated , or bulky , the combination of tumour necrosis factor ( TNF ) with melphalan and interferon gamma yields a complete response rate of around 80 % . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cultured rat hepatocytes exposed to interferon gamma , interleukin 1 , and tumor necrosis factor alpha showed iNOS protein expression within peroxisomes as early as 4 hours after stimulation , as determined by colabeling for catalase or PMP 70 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Herein , we describe spontaneous tumor development in gene targeted mice lacking interferon ( IFN ) gamma and / or perforin ( pfp ) , or the immunoregulatory cytokines , interleukin ( IL ) 12 , IL 18 , and tumor necrosis factor ( TNF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A single oral administration of pirfenidone prior to lipopolysaccharide / D galactosamine challenge inhibited the production of circulating tumor necrosis factor alpha ( TNF alpha ) , interleukin 12 and interferon gamma , markedly enhanced that of interleukin 10 , and offered protection from subsequent lethal symptoms in a dose dependent manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the murine endotoxin shock model , pirfenidone potently inhibited the production of the proinflammatory cytokines , TNF alpha , interferon gamma , and interleukin 6 , but enhanced the production of the anti inflammatory cytokine , interleukin 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A higher percentage of gammadelta ( + ) T cells producing tumor necrosis factor alpha was found in old donors and centenarians , whereas no age related difference was observed in interferon gamma production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When CCB was used in combination with recombinant interferon gamma ( rIFN gamma ) , there was a marked cooperative induction of NO production , TNF alpha production and NF kappa B activation . ^^^ CONCLUSIONS : These findings demonstrate that CCB increases the production of NO and TNF alpha by rIFN gamma primed peritoneal macrophages and suggest that NF kappa B plays a critical role in mediating these effects of CCB . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This binding phenomenon inhibits the ability of calcineurin to activate the promotor region of the gene for IL 2 , IL 3 , IL 4 , IL 5 , interferon gamma , tumor necrosis factor alpha , and granulocyte macrophage colony stimulating factor , all of which participate in the early immune response and play a role in the pathogenesis of atopic dermatitis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , the NO donors dose dependently reduced SEB evoked PBMC proliferation and cytokine production ( i . e . , interferon gamma , tumor necrosis factor alpha ) but did not affect viability . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We evaluated clinical manifestations , laboratory findings ( white blood cell count , C reactive protein , erythrocyte sedimentation rate ) and serum cytokine [ interleukin ( IL ) 1 beta , IL 6 , IL 8 , interferon gamma and tumor necrosis factor alpha ] concentrations . ^^^ Other cytokine ( IL 1 beta , IL 2 , interferon gamma and tumor necrosis factor alpha ) values did not differ among adenovirus , influenza virus and RSV infections . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Certain inflammatory cytokines , tumor necrosis factor alpha ( TNF alpha ) and transforming growth factor beta ( TGF beta ) , and the Th type 1 cytokine interferon gamma ( INF gamma ) are abundant in CD . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokine considered to be the most relevant to this process is tumor necrosis factor alpha ( TNF ) , although other mediators such as interleukin ( IL ) 1 , IL 6 and interferon gamma have also been implicated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the mechanisms regulating Apo2L / TRAIL induced apoptosis in thyroid carcinoma cells , as well as the impact of insulin like growth factor ( IGF ) 1 , interferon gamma , and TNF alpha . ^^^ Conversely , interferon gamma and TNF alpha had a sensitizing effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A significant reduction of proliferative responses , as well as interferon gamma ( IFNalpha ) and tumour necrosis factor alpha ( TNFalpha ) secretion , was observed at concentrations of 200 microg / ml suggesting that GA may also exert immunomodulatory effects on mitogen and superantigen induced T cell stimulation in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Treatment of IL 10 gene deficient mice with VSL # 3 resulted in normalization of colonic physiologic function and barrier integrity in conjunction with a reduction in mucosal secretion of tumor necrosis factor alpha and interferon gamma and an improvement in histologic disease . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OBJECTIVE : To evaluate the effect of interferon gamma ( IFNgamma ) on interleukin 1beta ( IL 1 ) and tumor necrosis factor alpha ( TNFalpha ) promoted prostaglandin E ( 2 ) ( PGE ( 2 ) ) production and to investigate the interaction of IFNgamma and IL 1 on cyclooxygenase 2 ( COX 2 ) expression , as well as nuclear factor kappaB ( NF kappaB ) activation in human myometrial cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of TNFalpha was increased by FB ( 1 ) treatment in the lung of male mice and in the heart of female mice , whereas the expression of interferon gamma was unaltered . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lymphocytes from these TCR transgenic mice proliferate and make interferon gamma , interleukin ( IL ) 2 , tumor necrosis factor ( TNF ) alpha , and IL 10 when stimulated in vitro with GAD 65 peptide 286 300 , yet these TCR transgenic animals do not spontaneously develop diabetes , and insulitis is virtually undetectable . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Endothelial cells were treated without ( Control ) or with PGF2alpha ( 1 micro M ) , TNFalpha ( 100 ng / ml ) , interferon gamma ( IFNgamma , 200 IU / ml ) , and TNFalpha + IFNgamma for 24 and 48 h in the absence or presence of progesterone ( P 4 , 250 ng / ml ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PATIENTS AND INTERVENTIONS : Leukocyte suspensions obtained from 16 patients , 6 during early sepsis or septic shock and 10 during late sepsis or septic shock , were incubated with anti CD 14 and anti CD 2 or anti CD 3 monoclonal antibodies and then with intracytoplasmic anticytokine antibodies staining for interferon gamma , TNF alpha , IL 2 , IL 6 , IL 8 , IL 10 , and IL 12 and analyzed with a flow cytometer . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
No significant difference was detected among these treatments in the expression of hepatocyte growth factor , interferon gamma , tumor necrosis factor alpha , and class 2 major histocompatibility complex . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Arterial oxygenation , wet / dry ratio , myeloperoxidase , and proinflammatory cytokines ( interleukin 1 , tumor necrosis factor alpha , interferon gamma , and interleukin 2 ) were measured , and immunohistochemistry was performed . ^^^ All proinflammatory cytokines were decreased in the cotransfected group compared with those in all other groups ( interleukin 1beta , P < . 04 ; tumor necrosis factor alpha , P < . 002 ; interferon gamma , P < . 0001 ; interleukin 2 , P < . 03 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : The maximum number of binding sites ( Bmax ) and their affinity ( Kd ) for [ 3H ] rauwolscine , a selective alpha 2 AR antagonist , and the stimulated production of tumor necrosis factor alpha ( TNFalpha ) , the Th 1 like cytokine , interferon gamma ( IFNgamma ) , and the Th 2 like cytokines , interleukin 10 ( IL 10 ) and IL 5 , were measured in 35 university students a few weeks before ( baseline ) as well as on the day before a difficult , oral examination ( stress condition ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These findings were confirmed by site directed mutagenesis of the tumor necrosis factor ARE and RNA gel shifts on c fos , interferon gamma , and interferon beta ARE fragments . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The ability of A 172 cells to express iNOS was assessed by stimulating cells with interferon gamma ( IFNgamma ) , tumor necrosis factor alpha ( TNFalpha ) , interleukin 1beta ( IL 1beta ) , bacterial lipopolysaccharide ( LPS ) , L arginine , and tetrahydrobiopterin ( BH 4 ) in various combinations . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : T cell activation increased gastric and small bowel permeability through a pathway mediated by interferon gamma and tumor necrosis factor . ^^^ Analysis of intestinal cytokine mRNA levels ( interferon gamma and tumor necrosis factor ) indicated that relevant mucosal T cell function was preserved in alcohol fed mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Many cells expressing messenger RNA ( mRNA ) encoding for tumour necrosis factor alpha ( TNF alpha ) were demonstrated , but few cells expressing interferon gamma mRNA and none expressing interleukin 4 mRNA were detected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast to murine TNF ( mTNF ) , human TNF ( hTNF ) only induces lethality at extremely high doses of about 500 microg / mouse , whereas it still has a powerful antitumor activity in combination with interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of macrophages with rIFN gamma plus OD ( 1 mg / ml ) caused a significant increase in tumor necrosis factor alpha ( TNF alpha ) production ( 4 . 49+ / 1 . 43 ng / ml ) by enzyme linked immunosorbent assay . ^^^ The increased production of NO and TNF alpha from rIFN gamma plus OD stimulated cells was almost completely inhibited by pretreatment with 100 microM of pyrrolidine dithiocarbamate ( PDTC ) , an inhibitor of nuclear factor kappa B ( NF kappaB ) . ^^^ These findings demonstrate that OD increases the production of NO and TNF alpha by rIFN gamma primed macrophages and suggest that NF kappaB plays a critical role in mediating these effects of OD . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It has previously been demonstrated that the STAT 1 transcription factor plays a key role in apoptosis induced by the cellular regulatory factors interferon gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In western Kenya , where severe malarial anemia is much more common than cerebral malaria , the distributions of tumor necrosis factor ( TNF ) alpha , interleukin ( IL ) 10 , transforming growth factor ( TGF ) beta , IL 6 , and interferon ( IFN ) gamma alleles were examined in a cohort of young men . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , treatment with IXD alone or rIFN gamma plus IXD in peritoneal macrophages caused a significant increase in tumor necrosis factor alpha ( TNF alpha ) production . ^^^ These findings demonstrate that IXD increases the production of NO and TNF alpha by rIFN gamma primed macrophages and suggest that NF kappaB plays a critical role in mediating these effects of IXD . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using clinical specimens and a range of in vitro models that simulate homeostasis , wound healing , and malignant progression , this study sought to establish the patterns of TN C expression in normal and neoplastic bladder and to determine the role of exogenous transforming growth factor beta 1 ( TGFbeta 1 ) , interleukin 4 ( IL 4 ) , basic fibroblast growth factor ( bFGF ) , tumour necrosis factor alpha ( TNFalpha ) , and interferon gamma ( IFNgamma ) in the induction of TN C expression by bladder uro epithelial cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In spite of the fact that the 2 R . leguminosarum Cpn 60 proteins share 74 . 5 % amino acid sequence identity , it was found that Cpn 60 . 3 induced the production of interleukin ( IL ) 1beta , tumor necrosis factor alpha , IL 6 , IL 8 , IL 10 , and IL 12 , but not IL 4 , interferon gamma , or GM CSF ( granulocyte macrophage colony stimulating factor ) , whereas the Cpn 60 . 1 protein failed to demonstrate any cytokine inducing activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFNgamma ) and tumor necrosis factor alpha ( TNFalpha ) are potent immunostimulatory cytokines with demonstrated tumoricidal effects in a variety of cancers . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
FB 1 treatment caused an increased expression of TNFalpha , interferon gamma ( IFNgamma ) and interleukin ( IL ) 12 p 40 in liver without any changes in kidney or spleen , suggesting the localized site of their production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Addition of interferon gamma did not restore the effect of FO on TNF production capacity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This `` bystander hepatitis ' ' was dependent on tumor necrosis factor alpha and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To this end , we stimulated primary BEC with increasing amounts of the T helper cell 1 cytokines , interferon gamma ( IFNgamma ) , and / or tumor necrosis factor alpha ( TNFalpha ) and measured the quantity and forms of HLA class 1 release . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , phosphoantigen reactive 5 ( gamma ) 9 / V ( delta ) 2 T cell clones from healthy individuals were found to produce significant amounts of tumor necrosis factor in response to A fumigatus antigens . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Animal models and some clinical trials support the notion that inhibition of inflammatory mediators such as interleukin 1 ( IL 1 ) , tumor necrosis factor alpha , and interferon gamma may ameliorate or prevent GVHD . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We measured the general clinical characteristics and percentage of intracellular production of interleukin 1 ( IL 1 ) , interleukin 6 ( IL 6 ) , interleukin 12 ( IL 12 ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 4 ( IL 4 ) , and interferon gamma ( INF gamma ) in T lymphocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In cooperation with T cells , the biscFv specifically induced not only interferon gamma and tumor necrosis factor alpha , but also a significant increase of merozoite phagocytosis and growth inhibition of P falciparum in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Small studies have shown possible associations between poor response to infliximab and increasing mucosal levels of activated NF kappaB , homozygosity for the polymorphism in exon 6 of TNFR 2 ( genotype Arg196Arg ) , positivity for perinuclear antineutrophil cytoplasmic antibodies ( ANCA ) , and with the presence of increased numbers of activated lamina propia mononuclear cells producing interferon gamma and TNFalpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of Ki 67 in tumour cells induced to apoptosis by tumour necrosis factor alpha ( TNFalpha ) and interferon gamma ( IFNgamma ) was studied . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The peripheral CD4+ and CD8+ T cells expressing CX3CR1 predominantly produced interferon gamma and tumor necrosis factor alpha , and expressed cytotoxic molecules such as granzyme A and perforin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lower steady state messenger RNA ( mRNA ) levels for TNFalpha , interferon gamma ( IFNgamma ) , IL 18 , and IL 6 were observed in the joints of anti C 5 treated mice , and for IFNgamma and IL 6 in mice receiving Crry Ig , all in comparison with PBS treated mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Local IL 10 overexpression correlated significantly with decreased CD4+ and CD8+ responsiveness and Type 1 helper ( Th 1 ) cytokine gene ( IL 2 , interferon gamma , and tumor necrosis factor alpha ) expression level and correlated inversely with the allograft rejection grade . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The phenotype of mitogen activated protein kinase activated protein kinase 2 ( MK 2 ) knockout mice revealed the essential role of this enzyme in post transcriptional regulation of lipopolysaccharide induced expression of cytokines such as tumour necrosis factor ( TNF ) alpha , interleukin 6 and interferon gamma , at the level of mRNA stability and translation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CONCLUSIONS : Hepatocyte growth factor in MNP is not directly induced by interferon alpha , interferon gamma or tumour necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The frequency of cytokine ( interferon gamma , tumor necrosis factor alpha , interleukin 2 ( IL 2 ) , IL 4 , IL 5 , IL 10 and IL 13 ) producing cells was assessed in total T lymphocytes , CD 3 ( + ) CD 8 ( + ) and CD 3 ( + ) CD 4 ( + ) subsets . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The aggravated course of the infection could be attributed to a suppressed transcription and production of anti listerial , pro inflammatory cytokines and chemokines , namely interferon gamma , tumor necrosis factor , interleukin 12p40 , inducible nitric oxide synthase , murine monokine induced by interferon gamma , and interferon gamma inducible protein 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated some of the mechanisms triggering the apoptotic machinery in rat insulinoma RINm5F cells and human islets treated with IL 1beta plus interferon gamma plus TNFalpha and assessed the effects of 1 , 25 ( OH ) 2D3 in these processes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The interleukin 10rcolon ; interferon gamma ratio , measured in supernatants of stimulated splenocytes , shifted from 131 for TNF ( / ) LT ( / ) mice and 13 . 9 for TNF ( + / + ) LT ( + / + ) mice on day 8 to 0 . 11 for TNF ( / ) LT ( / ) mice and 11 . 66 for TNF ( + / + ) LT ( + / + ) mice on day 14 of infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
INTRODUCTION : Proinflammatory cytokines ( PIC ) ( interleukin 1beta , interferon gamma , and tumor necrosis factor alpha ) are released after intraportal islet transplantation lead to functional suppression and islet apoptosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This hypothesis suggests that some types of ' endogenous ' and ' organic ' depression may be related to IRS activation , such as an increased production of pro inflammatory cytokines , such as interleukin 1beta ( IL 1beta ) , IL 6 , tumour necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN ) gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Peripheral blood mononuclear cells from four species of Aotusmonkey were obtained and their mRNAs for interleukin 2 ( IL 2 ) , IL 4 , IL 6 , IL 10 , interferon gamma ( IFN ) , and tumor necrosis factor ( TNF ) alpha were characterized . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Their role on spontaneous and phytohemagglutinin ( PHA ) induced proliferation of human peripheral blood lymphocytes ( PBL ) and on production of interferon gamma ( INF gamma ) and tumor necrosis factor alpha ( TNF alpha ) were determined in vitro . ^^^ These preparations also have effect on the formation of INF gamma , TNF alpha and some immune activation markers such as neopterin ( Neo ) , beta 2 microglobulin ( beta2MG ) , and soluble receptor for interleukin 2 ( sIL 2R or sCD 25 ) in blood cells culture . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This was accompanied by reductions in both the basal and lipopolysaccharide stimulated secretion of TNF alpha and interferon gamma in colonic organ cultures from IL 10 / mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Ad IL 10 transduced bone marrow derived macrophages ( BMDM ) produced large amounts of IL 10 in culture , and their TNF alpha production was decreased in response to interferon gamma and LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OBJECTIVES : To evaluate the role of interleukins ( IL 1 , IL 6 ) , tumor necrosis factor alpha ( TNFalpha ) and for the first time interferon gamma ( IFNgamma ) and epidermal growth factor ( EGF ) in the pathogenesis of premature rupture of membranes ( PROM ) with and without confirmed intrauterine infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Fanconi anemia complementation group C ( Fancc ) deficient murine bone marrow progenitors demonstrate increased sensitivity to growth inhibition by interferon gamma ( IFNgamma ) , tumor necrosis factor alpha ( TNFalpha ) , and macrophage inflammatory protein 1alpha ( MIP 1alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine production by leukocytes ( production of tumor necrosis factor alpha , interferon gamma , interleukin 10 , and interleukin 12 ) was inversely related to the endotoxin level in the bedding , indicating a marked down regulation of immune responses in exposed children . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokines , including interleukin 1beta ( IL 1beta ) , IL 2 , IL 4 , IL 6 , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma , were measured by ELISA kits . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this article , we analyze the relationship between cytokine gene polymorphisms and in vitro tumor necrosis factor alpha ( TNFalpha ) , interferon gamma ( IFNgamma ) , and interleukin ( IL ) 10 and IL 13 production , both in liver transplant recipients and in healthy volunteers . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
During G CSF administration , we also studied the serum levels of various cytokines ( IL 1beta , osteoclastogenesis inhibitory factor [ OCIF ] , IL 6 , tumor necrosis factor alpha , transforming growth factor beta , interferon gamma , macrophage colony stimulating factor ) related to bone metabolism . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Transcripts for interleukin 1beta ( IL 1beta ) IL 2 , IL 4 , IL 5 , IL 10 , IL 12 , IL 13 , IL 15 , IL 18 , and for tumor necrosis factor alpha ( TNFalpha ) and interferon gamma ( IFNgamma ) were detected by reverse transcription polymerase chain reaction of extracted RNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both human interferon gamma ( IFNgamma ) and tumor necrosis factor alpha ( TNFalpha ) have been used to treat a variety of colon carcinoma cell lines and tumors in patients . ^^^ Generation of human interferon gamma and tumor Necrosis factor alpha chimeric TNT 3 fusion proteins . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neural injury is known to trigger inflammatory changes , including the synthesis of proinflammatory cytokines such as interleukin 1 beta ( IL1beta ) , tumor necrosis factor alpha ( TNFalpha ) , and interferon gamma ( IFNgamma ) [ G . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the presence of physalin B , macrophages stimulated with lipopolysaccaride , alone or in combination with interferon gamma , produced lower levels of tumour necrosis factor ( TNF ) alpha , interleukin 6 and interleukin 12 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human ASM cells in culture between the second and fifth passages were exposed to tumor necrosis factor alpha ( TNF alpha ) , interleukin 1beta , or interferon gamma , or bovine serum albumin ( controls ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The production of messenger RNA for interleukin ( IL ) 12 , interferon ( IFN ) gamma , tumor necrosis factor alpha , IL 4 , and IL 10 was measured by quantitative reverse transcription polymerase chain reaction . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When rat RPE J cells were treated with interferon gamma ( IFNgamma ) , tumor necrosis factor alpha ( TNFalpha ) and lipopolysaccharide ( LPS ) , and expression of the citrulline NO cycle enzymes and related enzymes was analyzed , iNOS and argininosuccinate synthetase were highly induced at both mRNA and protein levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Flow cytometry in pretransplant sera was used to detect AECA reactive with surface antigens on ABO and HLA typed primary cultures of arterial ECs , stimulated or not with tumor necrosis factor alpha ( TNFalpha ) or interferon gamma ( IFNgamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RNase protection assay and real time reverse transcription polymerase chain reaction showed that tumor necrosis factor alpha / beta , interleukin 12 , and interferon gamma were inhibited by mechlorethamine upon successful treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Intragraft mRNA expression of interleukin ( IL ) 12 subunits ( p 35 , p 40 ) at 24 hours after transplantation was significantly ( P < 0 . 05 ) down regulated by the addition of rhG CSF and was associated with suppression of interferon gamma levels on day 6 , although other proinflammatory cytokines ( tumor necrosis factor alpha , IL 1beta , IL 6 , IL 18 ) and anti inflammatory cytokines ( IL 10 , transforming growth factor beta ) were not . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The production of H . capsulatum induced interferon gamma and TNF alpha was assessed in 5 day cultures containing lymphocytes and alveolar macrophages or monocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Activation of the cells with the inflammatory cytokine tumour necrosis factor alpha ( TNFalpha ) and interferon gamma ( IFNgamma ) increased the expression of some but not all receptors over a period of 24 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We studied the ability of LPS and killed gram negative bacteria ( GNB ) to induce tumor necrosis factor ( TNF ) alpha and interleukin ( IL ) 10 , and of phytohemagglutinin ( PHA ) to induce interferon ( IFN ) gamma , in whole blood from patients with sepsis ( SP , n = 20 ) , patients with matched underlying disease and without sepsis ( control patients , n = 20 ) , and healthy volunteers ( HV , n = 20 ) . ^^^ Impaired production of interferon gamma and tumor necrosis factor alpha but not of interleukin 10 in whole blood of patients with sepsis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
P . amarus inhibited induction of interleukin ( IL ) 1beta , IL 10 , and interferon gamma in human whole blood and reduced TNF alpha production in vivo . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Coculture with glioma cells also affected the expression of cytokines in neutrophils , including IL 8 , interferon gamma , and tumor necrosis factor alpha to various extents . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This strategy established the requirement for p 38 activity for the lipopolysaccharide stimulated production of IL 10 , IL 1beta , and IL 6 by the monocytic cell WEHI 274 and the production of IL 6 and TNFalpha stimulated by ligation of the Fc gamma receptor of the mast cell MC / 9 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Fe ] ( 1 ) response was also observed in tumor necrosis factor alpha ( TNFalpha ) stimulated HMs and RAW264 . 7 cells treated with LPS and interferon gamma but not in primary rat hepatocytes or myofibroblastic cells exposed to LPS or TNFalpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The NO levels in the cultured salivary gland epithelial cells were increased by treatment with a combination of interferon gamma ( Ifng ) , interleukin 1 beta ( Il1b ) , and tumor necrosis factor alpha ( Tnfa ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These studies compared the apoptotic potential of various cytokines ( tumor necrosis factor alpha , TNFalpha ; interferon gamma , IFNgamma ; soluble Fas ligand , sFasL ) , a FAS activating antibody ( FasAb ) , and the luteolytic hormone prostaglandin F2alpha ( PGF2alpha ) on CL derived endothelial ( CLENDO ) cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Depletion of primary afferent nerve fibers by neonatal capsaicin treatment down regulated circulating levels of the proinflammatory cytokines tumor necrosis factor alpha ( TNFalpha ) and interferon gamma ( IFNgamma ) and protected mice from GalN / LPS induced liver injury . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : An intracellular flow cytometric technique was applied to measure interleukin 1beta ( IL 1beta ) , IL 6 , TNFalpha , IL 10 and IL 12 in monocytes and IL 2 , IL 4 and interferon gamma in T lymphocytes of 15 patients , before , after 24 h and after 6 months of therapy with monoclonal chimeric anti TNFalpha antibodies ( 3 mg / kg , bimonthly i . v . ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We demonstrated and quantified by immunohistochemistry epidermal Langerhans cells , CD34+ dermal dendrocytes ( DDs ) , and cells expressing TNFalpha , interferon gamma ( IFNgamma ) , IL 5 , and IL 10 in skin lesions of paracoccidioidomycosis ( PCM ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cultured ocular ECs and stromal cells were stimulated with various inflammatory mediators ( endotoxin ; TNFalpha ; interferon gamma ; interleukin ( IL ) 1alpha , 4 , 10 , 13 , 17 , and 18 ; and / or CD 40 ligand , or combinations thereof ) , with FKN mRNA being subsequently evaluated by cDNA array and / or RT PCR and FKN protein by enzyme linked immunoculture assay ( ELICA ) and / or by Western blot analysis . ^^^ All ocular microvascular ECs and stromal cultures expressed low FKN mRNA and / or protein levels , which were variably upregulated by endotoxin , TNFalpha , interferon gamma , IL 1alpha , and / or CD 40 ligand , but not by IL 18 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma and lipopolysaccharide upregulate TNF related apoptosis inducing ligand expression in murine microglia . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There was an early , though not significant , decrease in plasma levels of proinflammatory cytokines ( interleukins ( IL ) 1beta , IL 6 , IL 8 , interferon gamma ) , whereas plasma levels of TNFalpha remained near the sensitivity limit of the assay throughout the treatment course . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
All superantigens were capable of inducing significant amounts of the lymphokines interferon gamma ( IFNgamma ) , interleukin 2 ( IL 2 ) , IL 4 , IL 5 , IL 13 and tumor necrosis factor beta ( TNFbeta ) after 72 h of incubation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The therapeutic effects of VIP were associated with down regulation of both inflammatory and Th 1 driven autoimmune responses , including tumor necrosis factor alpha , interleukin 1 , and interleukin 6 in colon extracts and serum as well as interferon gamma by splenic and lamina propria CD 4 ( + ) T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OCE significantly inhibited the production of TNF alpha , IL 1beta and interferon gamma ( INF gamma ) , compared to absence of OCE . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our previous results showed that regulatory RNAs can induce the production of interleukin 1 , interleukin 2 , interleukin 8 , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma , and Fas ligand ( FasL ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We next investigated physiologic levels of the T cell cytokines tumor necrosis factor alpha ( TNFalpha ) , transforming growth factor beta ( TGFbeta ) , interferon gamma ( IFNgamma ) , and interleukin 17 ( IL 17 ) for their roles in MMP 13 induction . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Myoblast migration during transplantation may be enhanced by overlapping gradients of several effector molecules such as TNF alpha , interferon gamma ( INF gamma ) , and interleukins , released at the site of muscle injury . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Vascular contraction experiments were performed with intact rat aortic rings . iNOS , TNF alpha and TGF beta gene expression in rat macrophages stimulated in vivo with 3 % thioglycollate and in vitro with 100 ng / mL lipopolysaccharide and 10U / mL of interferon gamma were evaluated semiquantitatively by the retrotranscriptase polymerase chain reaction . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of tumour necrosis factor alpha ( TNF alpha ) , Bax alpha , Bak mRNA and TNF alpha protein was also detected in these cultures ; in addition , an enhancement in the rate of apoptotic cells ( and of TNF alpha release ) was noted when interferon gamma was added to the wells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In order to clarify a potential contribution of inflammatory cytokines to this process , we have studied the modulation of integrin expression and adhesion of immature human mast cells ( HMC 1 ) to extracellular matrix proteins by interleukin 6 , tumor necrosis factor alpha , interferon alpha and interferon gamma . ^^^ In contrast , different concentrations of tumor necrosis factor alpha , interferon alpha , interferon gamma , and dexamethasone ( 10 8 10 10 M ) inhibited expression of adhesion receptors by up to 60 % , reaching significance for some but not all integrins . ^^^ Functional significance of these findings was proven in adhesion assays using fibronectin , laminin , and vitronectin , with interleukin 6 causing significant enhancement of adhesion in all cases , tumor necrosis factor alpha and dexamethasone inducing significant reduction of adhesion to fibronectin and laminin , and interferon gamma significantly inhibiting adhesion to fibronectin only . ^^^ These data show that interleukin 6 stimulates mast cell adhesion to extracellular matrix and thus allows for the accumulation of the cells at tissue sites by enhancing integrin expression , whereas tumor necrosis factor alpha , interferon alpha , interferon gamma , and dexamethasone downmodulate this process . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although its molecular mechanisms of action have not yet been elucidated , thalidomide and the IMiDs affect a variety of cytokines and inflammatory mediators including tumor necrosis factor alpha ( TNFalpha ) , interleukin ( IL ) 1beta , interferon gamma ( IFNgamma ) , IL 6 , IL 10 , IL 12 , and COX 2 and angiogenesis factors such as vascular endothelial growth factor ( VEGF ) and its receptor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In a macrophage cell line ( RAW 264 . 7 ) activated with interferon gamma plus lipopolysaccharide , both isoflavones were found to inhibit NO production and tumour necrosis factor alpha ( TNF alpha ) secretion dose dependently , but they did not affect mRNA levels for inducible nitric oxide synthase and cyclo oxygenase 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Murine bone marrow macrophages treated in vitro with F . novicida LPS produced IL 12 and TNF alpha , but did not produce detectable interferon gamma , IL 10 , or nitric oxide ; in contrast , murine macrophages treated with F . tularensis LVS LPS produced none of these mediators . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
At equivalent in vivo replicative history , the 2 subsets express high levels of Fas / CD95 and CD11a , as well as increasing levels of effector mediators such as granzyme B , perforin , interferon gamma , and tumor necrosis factor alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Secretion of interferon gamma , interleukin 10 , and tumor necrosis factor alpha was measured in the culture supernatants . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Each cell type was loaded with 2 , 7 dichlorodihydrofluorescein and then simultaneously activated with human cytokines ( tumor necrosis factor alpha , interleukin 1beta , and interferon gamma ) and hemoglobin and inhibited with and without equimolar concentrations of each steroid treatment or nitric oxide ( NO ) synthesis inhibitors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Twelve weeks of etanercept treatment induced a significant increase in the number of interferon gamma ( IFNgamma ) positive ( 14 . 2 % ( 9 . 6 19 . 5 % ) before 5 24 . 4 % ( 13 . 4 36 . 4 % ) after ) and TNFalpha positive CD4+ T cells ( p=0 . 008 for both cytokines ) and IFNgamma positive ( 37 . 5 % ( 19 . 0 45 . 4 % ) before 5 52 . 9 % ( 33 . 2 60 . 0 % ) after ) and TNFalpha positive CD8+ T cells ( p=0 . 008 for both cytokines ) upon phorbol myristate acetate / ionomycin stimulation , but not in the placebo group . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This technique was used to monitor the transcription patterns of mRNA encoding TNF alpha , IL 1beta , and Interferon gamma in human cell lines or primary PBMC treated with inducers such as PMA , ionomycin , and endotoxin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Studies using mouse axotomised facial motoneuron model show a strong and highly selective entry of CD3+ lymphocytes into the affected nucleus , with a maximum at Day 14 , which coincides with the peak of neuronal cell death , microglial phagocytosis , and increased synthesis of interleukin 1 beta ( IL1beta ) , tumour necrosis factor alpha ( TNFalpha ) and interferon gamma ( IFNgamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Exposure of A 498 cells to a cytokine mixture consisting of interferon gamma , interleukin 1 beta and tumor necrosis factor alpha ( TNF alpha ) increased nitrite production , iNOS mRNA and protein expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the absence of T helper cells , exogenous fungal antigens activated memory CD8+ cells in a major histocompatibility complex class 1 restricted manner and CD8+ T cell derived cytokines tumor necrosis factor alpha , interferon gamma , and granulocyte / macrophage colony stimulating factor mediated durable vaccine immunity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The major clinical traits ( susceptibility , onset , and severity ) were assessed , and PG specific T and B cell responses , and the production of proinflammatory and antiinflammatory cytokines ( tumor necrosis factor alpha , interleukin 1 [ IL 1 ] , IL 6 , interferon gamma , IL 4 , IL 10 , and IL 12 ) were measured in 133 arthritic and 426 nonarthritic female and male F ( 2 ) hybrid mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A randomly selected subset of patients underwent analyses including : dual energy 10 ray absorptiometry , metabolic and endocrine assays , in vitro cytokine production testing [ interferon gamma , interleukin ( IL ) 2 , IL 4 , IL 10 , IL 12 , tumor necrosis factor alpha ( TNF alpha ) ] after appropriate stimulation ; T cell phenotyping , T helper function after stimulation with either tetanus toxoid , influenza antigen , allogeneic peripheral blood lymphocytes , and phytohemagglutinin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
YC 1 enhanced lipopolysaccharide and interferon gamma ( LPS / IFN gamma ) induced TNF alpha formation in a concentration and time dependent fashion . ^^^ However , further studies suggested that cyclic GMP did not mediate the potentiation of YC 1 on TNF alpha release , because ( a ) the sGC inhibitor and the protein kinase G inhibitor failed to block the effect ; and ( b ) the cyclic GMP analogues , on the contrary , concentration dependently diminished LPS / IFN gamma induced TNF alpha synthesis . ^^^ In summary , potentiation of TNF alpha release by YC 1 in LPS / IFN gamma activated alveolar macrophages is an additional mode of action of this compound that is independent of the elevation of cyclic GMP . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
During inflammatory skin disorders such as psoriasis , atopic dermatitis , and allergic contact dermatitis , epidermal keratinocytes overexpress large amounts of soluble epidermal growth factor receptor ligands in response to tumor necrosis factor alpha and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In human vascular SMCs , CX3CL1 transcripts were induced after 4h of stimulation with a combination of TNF alpha and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Reverse transcriptase polymerase chain reaction analysis of purified CD 103 ( + ) and CD 103 ( ) CD 4 ( + ) T cells showed production of tumor necrosis factor ( TNF ) alpha R 1 ( p 55 ) , TNF alpha R 2 ( p 75 ) , interferon gamma , interleukin 10 , and TNF alpha mRNA in both subpopulations . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There are many candidate genes , particular the cluster of interleukin 4 genes ( 5q31 33 ) , the major histocompatibility complex genes HLA D and the TNFalpha gene ( 6p21 23 ) , the gene of the receptor B chain with strong affinity for IgE ( 11q13 ) , the interferon gamma gene ( 12q15 24 ) , the genes of the alpha / gamma chains of the T receptor ( 14q11 ) , the IL 4 receptor gene ( 16p12 ) , and the metalloprotease gene ADAM 33 ( 20p13 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Salmonella septicemia in rheumatoid arthritis patients receiving anti tumor necrosis factor therapy : association with decreased interferon gamma production and Toll like receptor 4 expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , treatment of peritoneal macrophages with rIFN gamma plus SR caused a significant increase in tumor necrosis factor alpha ( TNF alpha ) production . ^^^ These findings demonstrate that SR increases the production of NO and TNF alpha by rIFN gamma primed macrophages and suggest that NF kappaB plays a critical role in mediating these effects of SR . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Because interferon gamma ( IFNgamma ) is also found at inflammatory sites , we determined its effect on monocyte MMPs in the presence or absence of TNFalpha and GM CSF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro , interferon gamma and TNF alpha have been shown to increase the sensitivity of cells to apoptosis induced by H . pylori . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here we show by cDNA array analyses that TRAIL gene expression is upregulated in pancreatic islets during the development of autoimmune type 1 diabetes in nonobese diabetic ( NOD ) mice and in Min 6 islet beta cells activated by TNF alpha + interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Thelper 1 type cytokines , such as interferon gamma , TNF alpha and interleukin 1beta increase the sensitivity of both normal and neoplastic thyrocytes to FasL and TRAIL . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Gastric cancer cell lines AGS and KATOIII were incubated with the cytokines interleukin 1beta , interferon gamma , tumor necrosis factor alpha ( TNF alpha ) , and hepatocyte growth factor over a period of 72 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Preincubation of VSMCs with TGF beta 1 for 24 h , but not with PDGF BB , interferon gamma , TNF alpha , nor PMA , increased alphavbeta 3 and alpha5beta1 expressions on VSMCs and enhanced gene delivery of molossin vector . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Myelomonocytic differentiation was induced by phorbol ester ( 12 o Tetradecanoyl phorbol 13 acetate , TPA ) , tumor necrosis factor alpha ( TNFalpha ) or interferon gamma ( INFgamma ) , or their combination . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Silibinin proved to be an immune response modifier in vivo , inhibiting intrahepatic expression of tumor necrosis factor , interferon gamma , interleukin ( IL ) 4 , IL 2 , and iNOS , and augmenting synthesis of IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mercury altered the expression of inflammatory cytokines ( tumor necrosis factor alpha , interferon gamma , and interleukin 12 ) , c myc , and major histocompatibility complex 2 , in various organs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Accordingly , the levels of interferon gamma , tumor necrosis factor alpha , interleukin 1beta , interleukin 2 , and interleukin 6 remained unvaried after exposure . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The present study examines the cytoplasmic production of interleukin ( IL ) 2 , 4 , 10 , tumour necrosis factor alpha ( TNFalpha ) , and interferon gamma ( IFNgamma ) by activated PB CD3+ T cells and compares them with the profiles observed with normal individuals . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We show results from a sample human system in which absolute levels of interferon gamma , TNF alpha , interleukin 2 and interleukin 10 are measured . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Coinjection of interferon gamma , tumor necrosis factor alpha , and interleukin 1alpha to up regulate endothelial cell adhesion molecule expression with CXCL 10 or CCL 5 induced an additive increase in lymphoblast migration . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Numbers of interferon gamma expressing intrahepatic lymphocytes ( IHL ) were similar in AdCMV lacZ infected , TNF deficient , TNFR 1 deficient , TNFR 2 deficient , and control mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our results confirm that interaction of TNF alpha with TNF RII but not TNF RI is directly costimulatory to TCR mediated T cell activation , thereby augmenting T cell proliferation , expression of T cell activation markers ( CD 25 , human leukocyte antigen DR , TNF RII ) , and secretion of cytokines such as interferon gamma and TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using a murine osteoclast precursor cell line as well as primary human osteoclast precursors , we demonstrate that pharmacologic levels of two PIs that are linked clinically to osteopenia , ritonavir and saquinavir , abrogate a physiological block to RANKL activity , interferon gamma mediated degradation of the RANKL signaling adapter protein , TRAF 6 ( tumor necrosis factor receptor associated protein 6 ) in proteasomes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We now extend these findings by showing that message for TNFalpha , as well as mRNA for interferon gamma ( IFNgamma ) and transforming growth factor beta1 / 2 ( TGFbeta 1 , TGFbeta 2 ) , is simultaneously increased . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
All patients received melphalan and TNFalpha ( four patients also received interferon gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Associated with proliferation , cytokines are secreted into culture supernatants constituted mainly of Interferon gamma , negligible amounts of TNF alpha , and no IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : T cells from CHF patients showed enhanced gene expression of chemokines , ligands in the tumor necrosis factor superfamily , as well as the inflammatory cytokines interferon gamma and interleukin 18 with similar pattern in ischemic ( n=5 ) and idiopathic cardiomyopathy ( n=9 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cell mediated immunity to MI was assessed by measuring in vitro secretion of cytokines ( interleukin 8 , tumor necrosis factor alpha , and interferon gamma ) from peripheral blood mononuclear cells or anticoagulated whole blood induced by culture with MI antigen . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Intratumoral injection with dl 309 resulted in markedly greater macrophage infiltration and expression of both tumor necrosis factor and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Proinflammatory cytokines , such as interleukin 1beta and tumor necrosis factor alpha , but not interferon gamma , enhanced the expression of BMP 2 and BMP 6 transcripts in FLS in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum levels of interferon gamma ( IF gamma ) , tumor necrosis factor alpha ( TNF alpha ) and autoantibodies ( a AT ) to these cytokines were investigated in patients with Parkinson ' s disease ( PD ) . ^^^ The increased levels of TNF alpha ( 50 % ) and IF gamma ( 35 % ) were found in PD patients . ^^^ There was close correlation between the serum level of TNF alpha and the manifestation of neurological symptoms ( r = 0 . 434 ; p < 0 . 05 ) , and between levels of IF gamma and the duration of this disease ( r = 0 . 4511 , p < 0 . 05 ) and patients age as well ( r = 0 . 4358 ; p < 0 . 05 ) . ^^^ The combined increase of levels of a AT to TNF alpha and IF gamma ( r = 0 . 91 , p < 0 . 01 ) close reverse correlation between duration of PD and levels of a AT to TNF alpha and IF gamma ( r = 0 . 4644 and r = 0 . 606 , respectively , p < 0 . 01 ) were also recognised . ^^^ The data obtained suggest the involvement of TNF alpha and IF gamma into the pathological process during PD , which requires further investigation in this direction . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Myocytes in culture were treated with 50 ng / ml recombinant tumor necrosis factor alpha ( TNFalpha ) , 2 ng / ml interleukin 1beta ( IL 1beta ) , interleukin 6 ( IL 6 ) , interferon gamma ( IFNgamma ) or diluent ( NaCl ) for 24 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Cryopreserved cord blood serum samples from 407 children were assayed for interleukins 4 , 5 , 6 , 10 , 12 , and 13 , interferon gamma , and tumour necrosis factor alpha ( TNFalpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
One small study using preoperative limb perfusion with TNF alpha melphalan and + / interferon gamma combined with postoperative radiotherapy in the case of marginal or positive surgical margin has shown excellent local control without enhanced morbidity . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Incubation of L 2 cells with a mixture of interferon gamma ( IFNgamma ) , lipopolysaccharide ( LPS ) , and tumor necrosis factor ( TNFalpha ) resulted in high levels of nitrite formation resulting from iNOS induction . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It also reduced colonic expression of tumor necrosis factor alpha , IL 1beta , and interferon gamma mRNA without affecting the production of transforming growth factor beta , IL 10 , and IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The aim of this study was to investigate the detailed pattern of mRNA expression of the pro inflammatory cytokines tumor necrosis factor alpha ( TNFalpha ) , TNF receptor type 1 ( TNF R 1 ) , interleukin 1beta ( IL 1beta ) , and interferon gamma ( IFNgamma ) , and of the inducible nitric oxide synthase ( iNOS ) and the basic fibroblast growth factor ( FGF 2 ) during prostaglandin ( PG ) F ( 2alpha ) induced luteolysis in the bovine corpus luteum ( CL ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cultured cells from MSRV negative subjects did not produce virus , whereas spontaneous MSRV release was detected in cultures from MSRV positive donors ; virus release was increased by interleukin ( IL ) 4 and IL 6 and , to a greater extent , by the detrimental cytokines interferon gamma and tumor necrosis factor ( TNF ) alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : MKN 45 cells were cultured with low dose anticancer drugs ( 5 fluorouracil ( 5FU ) + cisplatin ( CDDP ) ) or cytokines ( tumor necrosis factor alpha ( TNFAlpha ) + interferon gamma ( INFGamma ) ) to induce apoptosis , or subjected to freezing thawing to induce necrosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The ability of two cytokines , tumor necrosis factor alpha and interferon gamma , are evaluated as paracrine mediators of endothelial cell function during angioregression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The patient was determined to have a low production of interferon gamma ( INF gamma ) and tumor necrosis factor alpha ( TNF alpha ) when compared to normal values . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In summary , we established that a significant production of the monokines interleukin 6 , tumor necrosis factor apha , and interleukin 1 occurred during orthotopic liver transplantation whereas the lymphokines interferon gamma and interleukin 2 were not detected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Infection of mononuclear cells in vitro with Venezuelan equine encephalitis virus replicon particles ( VRPs ) resulted in a significant increase in CD 56 ( + ) T cells and in the expression of interferon alpha , tumor necrosis factor ( TNF ) alpha , and interferon gamma by CD 56 ( + ) but not CD 56 ( ) T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Incubation of Caco 2 human enterocyte like monolayers with cytomix , a mixture of interleukin 1beta , interferon gamma , and tumor necrosis factor , increased permeability to the fluorescent macromolecule , FITC labeled Dextran ( mol wt 4 , 000 Da ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This media contained significant amounts of tumor necrosis factor ( TNF ) alpha , interleukin ( IL ) 1beta , interferon gamma , and IL 6 , but no IL 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We show that the C5aR is expressed on human monocyte derived dendritic cells ( DC ) and can be down regulated by maturation stimuli such as tumour necrosis factor alpha ( TNF alpha ) , lipopolysaccharide ( LPS ) or CD40L and by the T helper 1 cytokine interferon gamma ( INF gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Animals implanted with MCF 7IL 1 alpha cells were cachetic , which correlated with increased leptin serum levels but not other known cachexia inducing cytokines such as IL 6 , tumor necrosis factor , or interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine gene polymorphisms were determined for transforming growth factor ( TGF ) beta 1 codon 10 ( TGFbetac 10 ; C to T ) , TGFbeta 1 codon 25 ( TGFbetac 25 ; G to C ) , tumour necrosis factor alpha ( TNFalpha ) promoter 308 ( G to A ) , interleukin ( IL ) 6 promoter 174 ( G to C ) , IL 10 promoter 1082 ( G to A ) , IL 10 promoter 819 ( C to T ) , IL 10 promoter 592 ( C to A ) and interferon gamma ( IFNgamma ) intron 1 +874 ( A to T ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment with PRS 211 , 092 significantly decreased Concanavalin A induced liver injury in mice that was accompanied by : 1 ) promotion of early gene expression of interleukin ( IL ) 6 and IL 10 that play a protective role in this model ; 2 ) induction of early gene expression of the suppressors of cytokine signaling ( SOCS 1 and 3 ) , followed by 3 ) inhibition of several pro inflammatory mediators , including IL 2 , monocyte chemoattractant protein 1 ( MCP 1 ) , IL 1beta , interferon gamma , and tumor necrosis factor alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , PC SPES decreased the production of proinflammatory cytokines and inducible enzymes , such as tumor necrosis factor ( TNF ) alpha , interleukin ( IL ) 1beta , IL 6 , cyclooxygenase 2 , as well as inducible nitric oxide synthase in RAW264 . 7 macrophages and peritoneal macrophages from C57BL / 6 mice after the cells were stimulated by either LPS or LPS and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here we studied how the proinflammatory cytokines tumor necrosis factor alpha ( TNFalpha ) and interferon gamma ( IFNgamma ) affect NPC growth , survival , differentiation , and migration . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukins 1 and 2 , tumor necrosis factor alpha , interferon gamma , and granulocyte macrophage colony stimulating factor were greatly increased , especially in the infant who died . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Much of the pathology of malaria is mediated by inflammatory cytokines ( such as interleukin 12 , interferon gamma , and tumor necrosis factor alpha ) , which are part of the immune response that kills the parasite . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
DEP and DEP organic extracts have been found to suppress alveolar macrophage function as demonstrated by reduced production of cytokines ( interleukin 1 [ IL 1 ] , tumor necrosis factor alpha [ TNF alpha ] ) and reactive oxygen species ( ROS ) in response to a variety of agents , including lipopolysaccharide ( LPS ) , interferon gamma ( IFN gamma ) , and bacteria . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This resulted in an elevated production of Th 1 cytokines , including interferon gamma and tumor necrosis factor alpha , and a reduction in the Th 2 cytokines IL 4 and IL 5 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CONCLUSIONS : Amphotericin B induces the production of TNF alpha , interferon gamma , and IL 1beta , which may potentiate its toxic effects . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Direct fluorescence conjugated antibodies directed against intracellular cytokines ( interferon gamma , tumour necrosis factor [ TNF ] alpha , interleukin 1 ) and their receptors ( CD 119 , CD120a , CD121a ) were used . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RT PCR analysis revealed that CPA administration resulted in impaired mRNA production by peripheral blood mononuclear cells ( PBMCs ) of several cytokines ( interferons alpha / beta , interferon gamma , TNFalpha , IL 15 , and IL 18 ) with anti HSV function . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
VZV IE 63 , CD1a , CD 3 , CD 4 , CD 8 , CD 56 , CD 68 , L 1 , HLA DR , HLA ABC , interleukin ( IL ) 6 , IL 10 , interferon gamma ( IFNgamma ) , tumor necrosis factor alpha ( TNFalpha ) , and ICAM 1 expressions were assessed on frozen sections using immunohistochemistry . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The systemic production of tumor necrosis factor alpha , interferon gamma , and interleukin 6 was attenuated in mice either by a cell permeant cyclized form of SN 50 peptide or by a transgene whose product suppresses the nuclear import of transcription factor nuclear factor kappa B in T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Results indicate the following : ( 1 ) there is no correlation between peak parasitemia control or survival and the induction of infection associated anemia , loss of body weight , liver pathology , reduced locomotor activity , and general morbidity ; ( 2 ) serum levels of TNF , interferon gamma , and interleukin 10 , which are known to affect survival , do not correlate with induction of pathology ; and ( 3 ) infection induced occurrence of lipopolysaccharide hypersensitivity does not correlate with survival . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CONCLUSIONS : Proinflammatory cytokines IL 1beta , tumor necrosis factor alpha , IL 6 ; T helper 1 cytokines INF gamma and IL 2 receptor ; and chemokines IL 8 , monocyte chemoattractant protein 1 , and RANTES are increased in patients with exertional heatstroke . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokines of interest were grouped according to Th 1 effector functions : interleukin 1 beta , interleukin 2 , interleukin 12 , tumor necrosis factor alpha , tumor necrosis factor beta , interferon gamma ( IL 1 beta , IL 2 , IL 12 , TNF alpha , TNF beta , INF gamma , respectively ) and Th 2 effector functions : interleukin 4 , interleukin 6 , interleukin 10 , transforming growth factor beta 1 ( IL 4 , IL 6 , IL 10 , TGF beta 1 , respectively ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Epidermal growth factor / transforming growth factor alpha strongly increased the astrocytic expression of long RPTPbeta and phosphacan and slightly the short RPTPbeta protein levels , while interferon gamma and tumor necrosis factor alpha reduced astrocytic levels of phosphacan , but not of the receptor forms . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , when sacrificed at the end of the study , fusidin treated rats had significantly lower blood levels of tumor necrosis factor alpha and interferon gamma compared with untreated controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The mechanisms regulating hypothermia are not fully understood , but cytokines such as tumor necrosis factor alpha ( TNF alpha ) , interleukins ( ILs ) and interferon gamma have been shown to induce or modulate hypothermia . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of rIFN gamma plus C . majus ( 1 mgmL ( 1 ) ) in macrophages caused a significant increase in tumour necrosis factor alpha ( TNF alpha ) production . ^^^ The increased production of NO and TNF alpha from rIFN gamma plus C . majus stimulated cells was almost completely inhibited by nuclear factor kappaB ( NF kappaB ) inhibitor , pyrrolidine dithiocarbamate ( 100 microM ) . ^^^ These findings demonstrated that C . majus increased the production of NO and TNF alpha by rIFN gamma primed macrophages and suggested that NF kappaB played a critical role in mediating the effects of C . majus . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
On the contrary , treatment of 3T3 L 1 adipocytes with lipopolysaccharide alone did not affect GCH mRNA expression level , whereas the combination of lipopolysaccharide , tumor necrosis factor ( TNF ) alpha , and interferon gamma induced the increase in expression levels of GCH mRNA and CD 14 mRNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , both activation marker expressions ( CD 25 and CD 69 ) and cytokine message levels ( interferon gamma , and tumor necrosis factor alpha ) on CD 8 ( + ) TIL decrease in the IL 2 therapy model . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor 70 80 , an adenoviral vector that encodes murine tumor necrosis factor alpha , and recombinant interferon gamma seem to be the most promising cytokines for use as immunoadjuvants for the treatment of pneumonia . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OBJECTIVE : To determine whether cytokine gene polymorphisms of interferon gamma ( IFNgamma ) , interleukin 6 ( IL 6 ) , IL 10 , tumor necrosis factor alpha ( TNFalpha ) , and transforming growth factor beta 1 ( TGFbeta 1 ) predispose subjects to the development of primary Sjgren ' s syndrome ( SS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MHC 1 IR was particularly strong on phagocytic microglia , induced by delayed neuronal cell death , and correlated with the induction of mRNA for tumor necrosis factor ( TNF ) alpha , interleukin ( IL ) 1beta , and interferon gamma and the influx of T lymphocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Gamma , delta T cell proliferation and activation ( interferon gamma [ IFNgamma ] and TNFalpha release ) was prevented by mevastatin or lovastatin , which inhibit HMG CoA reductase upstream of FPP synthase and prevent the synthesis of isopentenyl diphosphate / dimethylallyl diphosphate . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human BMC depleted of CD3+ cells were cultured with either : interleukin 4 ( IL 4 ) and granulocyte macrophage colony stimulating factor ( GM CSF ) , to maintain DC enriched populations in an immature state ( iAPC ) ; or an interferon gamma ( IFNgamma ) , tumor necrosis factor alpha ( TNF alpha ) , GM CSF , LPS , and IL 6 cocktail to promote the maturation of DC enriched APC ( mAPC ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inhibition of electrogenic sodium transport as well as beta and gamma ENaC mRNA expression could be mimicked in control colon by in vitro preexposure for 8 hours to tumor necrosis factor alpha and interferon gamma . ^^^ Promoter analysis revealed that down regulation of beta and gamma ENaC gene expression was primarily induced by tumor necrosis factor alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 18 induces synthesis and release of interferon gamma ( IFN gamma ) , interleukins 8 , 4 and 13 , tumor necrosis factor alpha ( TNF alpha ) and granulocyte / macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RECENT FINDINGS : Currently data suggest that cancer anorexia cachexia syndrome results from a multifactorial process involving many mediators , including hormones ( e . g . leptin ) , neuropeptides ( e . g . neuropeptide Y , melanocortin , melanin concentrating hormone and orexin ) and cytokines ( e . g . interleukin 1 , interleukin 6 , tumor necrosis factor alpha and interferon gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Silymarin itself slightly increased expression of hepatic TNF alpha ; however , it prevented the FB ( 1 ) induced increases in TNF alpha , TNF receptor 1 , TNF receptor associated apoptosis inducing ligand , lymphotoxin beta , and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
DNA was extracted from buccal brushings and assayed for tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFN gamma ) , interleukin ( IL ) 6 , IL 10 , and transforming growth factor ( TGF ) beta 1 genotypes using polymerase chain reaction sequence specific primer technology . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The secretion and mRNA expression of interferon gamma ( IFNgamma ) , interleukin ( IL ) 4 , IL 5 , and TNFalpha from phytohaemagglutinin ( PHA ) stimulated peripheral blood mononuclear cells was measured with an ELISA and RT PCR . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There is circumstantial evidence that proinflammatory cytokines like tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) could have disease promoting roles in multiple sclerosis ( MS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro , M . indica extract and mangiferin were tested on TNFalpha and NO production in activated macrophages ( RAW264 . 7 cell line ) and microglia ( N 9 cell line ) stimulated with LPS ( 10ng ml ( 1 ) ) and interferon gamma ( IFNgamma , 2U ml ( 1 ) ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
DESIGN AND METHODS : We evaluated burst forming units erythroid ( BFU E ) and colony forming units granulocyte macrophage ( CFU GM ) in bone marrow mononuclear cells ( BMMC ) from 5 PNH patients and 5 controls , alone or in the presence of transforming growth factor ( TGF ) beta , interferon ( IFN ) gamma , tumor necrosis factor ( TNF ) alpha , and specific antibodies . ^^^ In PNH , addition of TGF beta inhibited both BFU E and CFU GM ; IFN gamma and TNF alpha inhibited BFU E alone . ^^^ In patients cytokines modulated normal and mutated clones differently : TGF b reduced the number of PIG A and PIG A+ colony forming cells ( CFC ) , whereas TNF alpha and IFN gamma reduced PIG A+ CFC only . ^^^ Mutated clones were more resistant to the inhibitory effects of IFN gamma and TNF alpha , suggesting that PNH cells may have a growth advantage in an unfavorable microenvironment . . ^^^ Increased resistance of PIG A bone marrow progenitors to tumor necrosis factor a and interferon gamma : possible implications for the in vivo dominance of paroxysmal nocturnal hemoglobinuria clones . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
DNA was extracted from peripheral blood mononuclear cells , and polymerase chain reaction sequence specific primers ( PCR SSP ) analysis was performed on promoter sequences of transforming growth factor beta 1 ( TGF beta 1 ) , interleukin 6 ( IL 6 ) interleukin 10 ( IL 10 ) , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( INF gamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Ex vivo T cell generation of tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( IFN gamma ) from the patients was assessed before treatment and 6 to 8 wk after therapy . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Oral infection of susceptible mice with Toxoplasma gondii results in an acute lethal ileitis characterized by increased interferon gamma , tumor necrosis factor alpha , and inducible nitric oxide synthase ; homeostasis results from transforming growth factor beta production by intraepithelial lymphocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In human saphenous vein endothelial cells ( HSVECs ) , tumor necrosis factor alpha ( TNFalpha ) and bacterial lipopolysaccharide ( LPS ) , but neither interferon gamma ( IFNgamma ) nor interleukin 1beta ( IL 1beta ) , stimulate arginine transport . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Secretion of interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and interleukin ( IL ) 10 was determined using enzyme linked immunosorbent spot assay . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , IL 6 inhibited production of tumor necrosis factor alpha ( TNF alpha ) and interferon gamma inducible protein 10 ( IP 10 ) in DC , and DC driven allogeneic T cell proliferation in mixed lymphocyte reactions . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
All the PDCL showed resistance to Fas mediated apoptosis but were significantly sensitive to the pro apoptotic effect of inflammatory cytokines [ interleukin ( IL ) 1beta , tumor necrosis factor ( TNF ) alpha and interferon gamma ] . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The secretion of IL 2 , IL 5 , IL 6 , interferon gamma , and the inflammatory cytokines IL 1beta , IL 8 , and tumor necrosis factor alpha was not or was hardly affected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In particular , the induction of the adhesion molecule E selectin by either tumor necrosis factor alpha ( TNFalpha ) or Escherichia coli lipopolysaccharide ( LPS ) was reduced by more than 70 % in HUVECs , whereas inducible nitric oxide synthase ( iNOS ) induction was abolished in C 6 cells after exposure to interferon gamma in combination with LPS and TNFalpha , suggesting that the receptor inhibited a common step in the induction of each of these pro inflammatory genes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Circulating tumour necrosis factor ( TNF ) alpha , interferon gamma , interleukin ( IL ) 1beta , and IL 6 levels were higher in myeloma patients with overt bone disease , whose osteoblasts constitutively overexpressed Fas , DR4 / DR5 complex as receptors to TNF related apoptosis inducing ligand , intercellular adhesion molecule 1 ( ICAM 1 ) , and monocyte chemotactic protein 1 ( MCP 1 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Apoptosis was induced in insulinoma ( INS ) 1E cells , rat islets , and fluorescence activated cell sorting purified beta cells by oleate , palmitate , and / or cytokines ( IL 1beta , interferon gamma , TNFalpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stepwise regression analysis showed that there were significantly positive correlations between IL 1beta and IL 6 , IL 1beta and nitric oxide , IL 4 and tumor necrosis factor ( TNF ) alpha , IL 4 and leptin , IL 8 and IL 2 , and interferon ( IFN ) gamma and IL 6 , as well as significantly inversed correlations between IL 6 and IL 2 , IL 8 and interferon gamma , and leptin and TNF alpha in siblings , not in the children with diabetes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here we test the hypothesis that injection of pro inflammatory cytokines , tumour necrosis factor alpha ( TNFalpha ) and interferon gamma ( IFNgamma ) into the brain of animals in the prodromal phase of EAE significantly enhances inflammation in the central nervous system ( CNS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After stimulation for 3 h with PMA plus ionomycin , cellular responses were associated with surface TNFalpha expression on the majority of CD3+ T cells and secretion of Th 1 associated cytokines : interferon gamma , interleukin ( IL ) 2 , and to a lesser extent IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Therapeutic M ILP , with or without tumor necrosis factor alpha and interferon gamma ( T ( 1 ) M ILP ) , seems indicated in unresectable melanoma ( level 3 to 4 evidence ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Nuclear localization of p 65 , as well as levels of IL 1beta , IL 6 , TNF alpha , and interferon gamma , were significantly reduced in the paw tissue of TPCA 1 and etanercept treated mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RECENT FINDINGS : Human conjunctival epithelial cells respond to tumor necrosis factor alpha , interleukin 1 beta , and interferon gamma individually and in combination . ^^^ Conjunctival epithelial cells release interleukin 8 in response to interleukin 1 beta and tumor necrosis factor alpha but not interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , mDC of patients showed a reduced capacity to produce tumor necrosis factor alpha after a stimulus with synthetic double stranded RNA and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Alone , interferon gamma ( IFNgamma ) but not IL 1beta or TNFalpha generated nitrite in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This work describes single nucleotide polymorphism of tumor necrosis factor ( TNF ) alpha , interferon gamma , interleukin ( IL ) 6 , transforming growth factor beta 1 , and IL 10 in patients with dengue virus infections and analyzes their relation with clinical manifestations of the disease . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of chemokine receptor ( CCR 4 , CCR 5 ) and cytokine ( interleukin ( IL ) 2 , IL 10 , interferon gamma ( IFNgamma ) , tumour necrosis factor alpha ( TNFalpha ) , and transforming growth factor beta ( TGFbeta ) ) specific mRNA in intestinal biopsy samples from patients with RA was examined . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The analysis at the end of the observation period included the histopathologic assessment of the intestine and the localization of TNF and interferon gamma ( IFNgamma ) expressing cells . ^^^ In the presence of tmTNF , the absence of secreted TNF did not affect frequency and distribution of TNF and interferon gamma messenger RNA ( mRNA ) expressing cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The secretion of tumor necrosis factor alpha , interferon gamma and interleukin 2 by opsonized PBMN was significantly reduced after 24 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression levels of interferon gamma , tumor necrosis factor ( TNF ) alpha and interleukin ( IL ) 12 mRNA were lower in GK / Jcl diabetic rats than those in control Wistar rats . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This ApN induction was reproduced in C2C12 myotubes cultured for 48 h with a proinflammatory cytokine combination , interferon gamma + TNFalpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increased systemic levels and sensitivity of cells to the inflammatory cytokines interferon gamma and tumor necrosis factor may contribute to the disease . ^^^ This is reminiscent of the pancreatitis and islet neogenesis that occur in mice that transgenically overexpress interferon gamma and / or tumor necrosis factor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha and interferon gamma significantly stimulated follistatin expression but down regulated expression of gremlin . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When measured in real time , peptide antigen and the cytokines , interleukin 12 ( IL 12 ) and IL 18 , independently regulate the on / off kinetics of protective ( interferon gamma , tumor necrosis factor alpha ) and immunomodulatory ( IL 2 , CD40L ) cytokine production by activated T cells and memory T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin 1alpha ( IL 1alpha ) , IL 1beta , IL 2 , IL 4 , IL 5 , IL 8 , IL 10 , IL 12p35 , IL 12p40 , IL 15 , interferon gamma ( IFNgamma ) , and tumor necrosis factor alpha ( TNFalpha ) mRNA expression was quantified by real time PCR . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Incubation of AS and control PBMC with phytohaemagglutinin ( PHA ) led to upregulation of TGFbeta 1 , interleukin 10 , tumour necrosis factor alpha ( TNFalpha ) and interferon gamma ( IFNgamma ) assessed by ELISA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The responses of plasma cortisol and adrenocorticotropic hormone ( ACTH ) were examined to intravenous injection of recombinant bovine tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( INF gamma ) in Holstein cows . ^^^ INF gamma induced dose dependent rises in the plasma levels of both cortisol and ACTH , while TNF alpha induced comparable plasma cortisol responses with much smaller rises in plasma ACTH . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
OBJECTIVE : The objective of this study was to evaluate the genetic profiles of selected cytokines ( transforming growth factor beta 1 , tumor necrosis factor alpha , interleukin 6 , interferon gamma , and interleukin 10 ) in systemic lupus erythematosus and the contributions of human leukocyte antigen ( HLA ) DRB 1 and DQB 1 alleles to susceptibility for this disease . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The clinical characteristics associated with this disease result from overproduction of cytokines including interferon gamma ( INF gamma ) , tumor necrosis factor alpha ( TNF alpha ) , interleukin 6 ( IL 6 ) . ^^^ In each organ , mRNA levels of inflammatory cytokines ( INF gamma , TNF alpha , IL 6 ) were highly detected compared with beta Actin mRNA levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Previous studies revealed that these late microglial changes are accompanied by a strong increase in the expression of proinflammatory cytokines such as interleukin 1 beta ( IL1beta ) tumor necrosis factor alpha ( TNFalpha ) and interferon gamma ( IFNgamma ) [ J . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interestingly , recipients of cdd CD8+ T cells demonstrated significantly greater serum levels of interferon gamma and tumor necrosis factor alpha and histopathologic damage from GVHD than wild type ( wt ) T cells on day 30 after allogeneic bone marrow transplantation ( P < . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The regulatory signal ( s ) for MHC 1 expression in normal neurons remains unresolved but among tentative molecules are cytokines such as interferon gamma ( INF gamma ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The release of interferon gamma and tumor necrosis factor alpha by these immune effector cells is enhanced by SD 208 , whereas the release of interleukin 10 is reduced . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Polymorphic markers identified in the horse genes encoding the interleukin 12 p 40 subunit , interferon gamma , tumor necrosis factor receptor 1 , and inducible nitric oxide synthase were identified and tested , along with additional markers , for associations with two important horse infections : Rhodococcus equi and Lawsonia intracellularis . ^^^ Marginal associations with tumor necrosis factor alpha , interferon gamma , and other genes suggested that variations in immune response related genes could underlie the phenotypic variation observed . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Eighteen hydrosalpingeal fluids were compared with 15 follicular fluids and serum samples regarding the presence of interleukin 8 ( IL 8 ) , IL 12 , IL 1alpha , epidermal growth factor ( EGF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , leukemia inhibitory factor ( LIF ) , tumor necrosis factor alpha ( TNFalpha ) , interferon gamma ( IFNgamma ) , and transforming growth factor beta 2 ( TGFbeta 2 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Assessment of the cytokine released from the 2 patients with CD 4 lymphopenia revealed a defective production of the proinflammatory cytokines interferon ( IFN ) gamma and tumor necrosis factor ( TNF ) but not of the anti inflammatory cytokine interleukin 10 ( IL 10 ) . ^^^ CONCLUSIONS : Refractory meningitis may be due to defective TNF and IFN gamma production , and IFN gamma treatment may be useful in patients with an impaired cellular immune response and refractory cryptococcal meningitis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Profiles of whole blood cytokine responses to parasite antigens ( for interleukin [ IL ] 5 , IL 13 , IL 10 , IL 12p40 , tumor necrosis factor alpha , and interferon gamma ) , assayed before treatment , were significantly related both to an overall measure of host susceptibility and to susceptibility to reinfection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The following cytokines were measured : interleukin 1beta ( IL 1beta ) , IL 2 , IL 4 , IL 5 , IL 6 , IL 7 , IL 8 , IL 10 , IL 12 , IL 13 , IL 17 , interferon gamma ( IFNgamma ) , tumor necrosis factor alpha , granulocyte colony stimulating factor ( G CSF ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , monocyte chemoattractant protein 1 ( MCP 1 ) , and macrophage inflammatory protein 1beta ( MIP 1beta ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increased steady state levels of messenger RNA ( mRNA ) for interferon gamma ( IFNgamma ) , TNFalpha , and interleukin 1beta ( IL 1beta ) , and lower levels of IL 1 receptor antagonist ( IL 1Ra ) mRNA were present in the joints of the EC SOD KO mice compared with the WT mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mouse and human B 7 H1+ NK cells induced proliferation of T cells and production of interferon gamma and tumor necrosis factor alpha in vitro , and in vivo blocking of B 7 H1 inhibited the protective effect of vaccination . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When the bacteria were applied apically to the CACO 2 cell layer , the production of TNF alpha , IL 12 , IL 1beta , IL 8 , IL 6 , IL 10 , TGF beta and interferon gamma was pronouncedly lower as compared to the bacterial stimulation of leucocytes beneath the CACO 2 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Elispot for the detection of interferon gamma appears to be more sensitive and reliable than measurement of tumor necrosis factor alpha or granzyme B . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In these studies , the effects of activating retinoid receptor ( retinoic acid receptor ( RAR ) and retinoid 10 receptor ( RXR ) ) and peroxisome proliferator activated receptor ( PPAR ) gamma , which belong to the steroid lipid nuclear receptor family , on tumor necrosis factor ( TNF ) alpha production and inducible nitric oxide synthase ( iNOS ) gene expression by stimulated U 937 and SVG cells , respectively , were examined . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both gadolinium and FB 1 treatments individually increased the expression of selected cell signal factors ; e . g . , TNFalpha , TNF receptor 1 , TNF related apoptosis inducing ligand , lymphotoxin beta , interferon gamma , and transforming growth factor beta 1 ; gadolinium chloride did not alter FB 1 induced expression of the above genes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Regulation of inhibitory and activating FcgammaR on monocytes by proinflammatory ( interferon gamma [ IFNgamma ] , tumor necrosis factor alpha [ TNFalpha ] ) and antiinflammatory ( interleukin 4 [ IL 4 ] , IL 10 ) cytokines was studied . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Besides , these subsets differ in their capacity for tumour necrosis factor alpha ( TNF alpha ) production in response to lipopolysaccharide + interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , cytokine synthesis of interferon gamma , interleukin 10 ( IL 10 ) , tumor necrosis factor a , and IL 5 by the patient ' s rice stimulated PBLs was elevated , indicating a TH1 / TH2 cell response to rice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Recipients were wild type mice treated with alpha NK cell antibodies or knockout strain mice lacking expression of CD 8 , perforin , Fas ligand , or 1 of the following cytokines : tumor necrosis factor alpha , transforming growth factor beta , interferon gamma , interleukin 4 , or interleukin 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
As compared to wild type controls , CD 31 deficient mice showed enhanced vascular permeability ; increased apoptotic cell death in liver , kidney , and spleen ; and elevated levels of serum tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( IFNgamma ) , MCP 1 , MCP 5 , sTNRF , and IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cultured human astrocytes secreted functionally active BAFF after stimulation with interferon gamma and TNF alpha via a furin like protease dependent pathway . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CXCL 10 , although not basally detected in cultured hZFC , was strongly induced by interferon gamma and synergistically increased by TNF alpha addition . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
By using enzyme linked immunosorbent assay , we measured the levels of tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( INF gamma ) , interleukin 6 ( IL 6 ) , and interleukin 10 ( IL 10 ) in the cervical secretions of 120 cytologically normal or equivocal and 91 abnormal Japanese women . ^^^ The levels of INF gamma and TNF alpha ( Th 1 cytokines ) correlated negatively with those of IL 6 and IL 10 ( Th 2 cytokines ) in HPV+ normal and LSIL subjects , whereas no such correlation was observed for HSIL . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The extent of THP 1 cell activation was determined by measuring mRNA levels of TNF alpha and IL 1beta , cyclooxygenase 2 ( COX 2 ) , macrophage inflammatory protein 1alpha ( MIP 1alpha ) , monocyte chemoattractant protein 1 ( MCP 1 ) , and interferon gamma inducible protein 10 ( IP 10 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Hyperthermic Limb Perfusion ( HILP ) with Tumor Necrosis Factor alpha ( TNFalpha ) and interferon gamma ( IFNgamma ) was pioneered by Linard and Lejeune in 1988 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Helminths can impede interleukin 12 , interferon gamma , and tumor necrosis factor alpha release and promote interleukin 10 , transforming growth factor beta , and regulatory T cell production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study showed that LPS injection in control rats caused ( 1 ) a time dependent increase in blood lymphocyte enumeration and differentiation , and ( 2 ) a sequential increase the pro inflammatory ( interleukin 1beta ( IL1beta ) , tumor necrosis factor alpha ( TNFalpha ) , interferon gamma ( INTgamma ) , and inducible nitric oxide synthase ( iNOS ) ) and anti inflammatory ( interleukin 4 ( IL 4 ) , corticotropin releasing factor ( CRF ) , and blood corticosterone ( Cort ) ) cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Hepatic natural killer T cell depletion leads to Th 1 polarization of hepatic cytokine production , increasing tumor necrosis factor alpha and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Decreases in the secretion of interleukin 12 , interferon gamma , and tumor necrosis factor alpha by mononuclear cells of the colonic lamina propria accompanied clinical improvement in patients receiving anti interleukin 12 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Major histocompatibility complex ( MHC2+ ) perivascular macrophages in the axotomized facial motor nucleus are regulated by receptors for interferon gamma ( IFNgamma ) and tumor necrosis factor ( TNF ) . ^^^ Transection of the facial nerve led to a gradual but strong upregulation in the number of MHC2+ cells , beginning at day 2 and reaching a maximum 14 days after axotomy , correlated with the induction of mRNA for tumor necrosis factor ( TNF ) alpha , interleukin ( IL ) 1beta and interferon gamma ( IFNgamma ) and a peak in neuronal cell death . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The icv administration of FB 1 induced expression of tumor necrosis factor alpha , interleukin 1beta , interleukin 6 and interferon gamma after both doses , assayed by the real time polymerase chain reaction . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When activated , NKT cells rapidly release suppressive cytokines , such as interleukin 4 ( IL 4 ) , IL 10 , and IL 13 , as well as inflammatory cytokines , such as interferon gamma and tumor necrosis factor alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
One hundred eleven men with African American ancestry were tested for G6PD deficiency ( Type A 202 / 376 ) and for the cytokine gene promoter polymorphisms of IL 10 ( 1082 G / A , 819 T / C , and 592 A / C ) , tumor necrosis factor ( TNF ) alpha ( 308 G / A ) , transforming growth factor ( TGF ) beta 1 ( C / T codon 10 and C / G codon 25 ) , IL 6 ( 174 G / C ) , and interferon ( IFN ) gamma ( +874 A / T ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Interleukin ( IL ) 5 , IL 6 , and tumor necrosis factor ( TNF ) induced an eosinophil shape change , whereas interferon ( IFN ) gamma significantly inhibited the shape change . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Fractalkine messenger RNA expression was upregulated in two cultured BECs on treatment with lipopolysaccharide and Th 1 cytokines ( interleukin 1beta , interferon gamma , and tumor necrosis factor alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Levels of cytokines interleukin 2 , tumor necrosis factor alpha , and interferon gamma at the end of the conditioning regimen were lower in the Cy Bu group , while liver enzymes were higher after the Bu Cy regimen . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment with Ro significantly decreased expression of interferon gamma ( IFNgamma ) , interleukin 17 ( IL 17 ) , tumor necrosis factor alpha , and the inducible nitric oxide synthase mRNA in the colon , whereas expression of IL 12p40 was unchanged . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RTE 2 proliferation was also inhibited by transforming growth factor beta ( TGFbeta ) and tumor necrosis factor alpha ( TNFalpha ) , whereas interferon gamma ( IFNgamma ) was a weak inhibitor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Gene families with evidence of HGT included several involved in host immune defense mechanisms ( the MHC class 1 , interleukin 10 , interleukin 24 , interleukin 18 , the interferon gamma receptor , and tumor necrosis factor receptor 2 ) and others ( glutaredoxin and glutathione peroxidase ) involved in resistance of cells to oxidative stress . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The treatment of PMNLs with the combination of IFN gamma and GM CSF significantly increased the release of tumor necrosis factor alpha in response to R . microsporus and A . corymbifera hyphae . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cells were next activated with 1 ng / mL of tumor necrosis factor alpha ( TNF alpha ) for 1 hour to determine macrophage chemotactic factor ( MCP 1 ) and interferon gamma activated protein ( IP 10 ) mRNA levels using reverse transcriptase polymerase chain reaction ( RT PCR ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serial measurements of interferon gamma , tumor necrosis factor alpha , transforming growth factor beta ( TGFbeta ) , interleukin 10 ( IL 10 ) , and IL 1 were done at each time point . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , transgenic mice overexpressing GM CSF in the lungs but with a lack of GM CSF in other organs ( GM+ ) are able to recruit lymphocytes and to express a TH 1 response with production of TNF alpha and interferon gamma in the lungs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This is restricted by the class 2 molecule of the major histocompatibility complex ( MHC ) , and results in the production of a cytokine cascade that includes interleukin 2 ( IL 2 ) , interferon gamma ( IFNgamma ) , tumor necrosis factor ( TNF ) and many other cytokines , including IL 6 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In order to induce inflammatory responses , human peripheral blood mononuclear and / or polymorphonuclear leukocytes were stimulated with lipopolysaccharide ( LPS ) and interferon gamma ( IFN ( gamma ) ) and the production of PGE 2 , interleukin 8 ( IL 8 ) , and TNF alpha was determined . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , the antiviral activity of bac rwIFN gamma was inhibited by anti wIFN gamma antibodies and the cytotoxicity of bac rwTNF alpha neutralized by cross reactive antibodies to murine TNF alpha . ^^^ Comparison of biological activity of recombinant woodchuck interferon gamma and tumor necrosis factor alpha produced in baculovirus and Escherichia coli expression systems . ^^^ The full length cDNAs of recombinant woodchuck interferon gamma ( rwIFN gamma ) and woodchuck tumor necrosis factor alpha ( rwTNF alpha ) were cloned into baculovirus transfer vectors and expressed in insect Sf 9 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Upon NK / DC interaction , resulting in NKp 30 engagement , NK cells produced tumor necrosis factor alpha ( TNFalpha ) ( and interferon gamma [ IFNgamma ] ) that , in turn , promoted DC maturation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neutralization of MIF also down regulated the ex vivo secretion of the proinflammatory mediators , TNF alpha , interferon gamma , and nitric oxide , while augmenting that of the antiinflammatory cytokine , IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Crohn ' s disease is a T helper type 1 response immune disease characterized by increased production of interleukin 12 tumor necrosis factor a ( TNF a ) , and interferon g . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines , including tumor necrosis factor alpha , interferon gamma , and interleukins 4 , 6 , 8 , 10 , and 12 , were assayed using commercial ELISA kits . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The changes in mRNA expression level of interleukin 2 ( Il 2 ) , Il 4 , tumor necrosis factor alpha ( Tnf ) , interferon gamma ( Ifng ) , and transforming growth factor beta ( Tgfb ) were examined . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma , tumour necrosis factor ( TNF ) alpha , interleukin ( IL ) 12 and IL 18 play a critical role in driving the Th 1 commitment in the course of granulomatous process . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Multivariate logistic regression modeling demonstrated that the adjusted odds ratio and 95 % confidence interval for recurrent ( > or =2 ) early rejections ( 0 3 months ) increased linearly for high ( H ) > intermediate ( 1 ) > low ( L ) interferon gamma ( 1 . 8 ; 1 . 1 3 . 2 ) and tumor necrosis factor ( TNF ) alpha ( 3 . 0 ; 1 . 3 6 . 9 ) genotype , whereas every 1 microg / L increase in the cyclosporine A level was protective ( 0 . 991 ; 0 . 984 0 . 999 ) . ^^^ CONCLUSIONS : High transforming growth factor beta 1 producer phenotype seems to be protective against acute inflammation , whereas H and 1 interferon gamma , TNFalpha , and interleukin 10 producer genotypes correlate with adverse outcomes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine stimulation assays were performed on local lymph node cells and spleen cells , and levels of interleukin 10 ( IL 10 ) , IL 4 , tumor necrosis factor alpha ( TNFalpha ) , and interferon gamma ( IFNgamma ) were determined . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In group 5 cotransfection suppressed endogenous interleukin 2 but not interferon gamma and tumor necrosis factor alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , a predominance of a T helper 1 pattern of cytokine production ( interferon gamma and tumor necrosis factor alpha ) with decreased secretion of T helper 2 associated cytokines ( interleukin 5 and interleukin 10 ) was also observed at the single cell level . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytokine interferon gamma ( IFNgamma ) is not normally produced in the CNS , but TNFalpha levels are enhanced if it is present . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Transforming growth factor beta 1 ( TGFbeta 1 ) , TGFbeta 2 , TGFbeta 3 , tumor necrosis factor alpha , interleukin 6 , and interferon gamma mRNA were measured . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Psoriasis is believed to be characterized by a type 1 cytokine pattern ; interferon gamma , interleukin ( IL ) 2 and tumour necrosis factor ( TNF ) alpha are predominantly expressed in this disorder . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma ( IFNgamma ) , tumour necrosis factor alpha ( TNFalpha ) , interleukin 1 beta ( IL 1beta ) , interleukin 10 ( IL 10 ) and interleukin 4 ( IL 4 ) secretion in supernatants were measured by ELISA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The activities of monocyte lysophosphatidylcholine acyltransferase and coenzyme A independent transacylase are changed by the inflammatory cytokines tumor necrosis factor alpha and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , peripheral blood CD 4 ( + ) T cell phenotype and activation status were evaluated by flow cytometry before treatment and at 2 , 4 , and 12 weeks using CD 69 , chemokine receptor ( CCR 4 , CCR 5 , and CXCR 3 ) , and intracellular cytokine ( tumor necrosis factor alpha , interferon gamma , and interleukin 10 ) expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation of macrophages with INF gamma , TNF alpha , and LPS resulted in an increase in Chit activity as well as the levels of CHIT 1 mRNA as measured by quantitative real time PCR . ^^^ The data presented in this article show that Chit plays a role in the response to the activation of INF gamma , TNF alpha , and LPS driven macrophages , suggesting that the production of Chit by macrophages could have biological relevance in the immune response . . ^^^ To assess this hypothesis , we evaluated the CHIT 1 mRNA levels in human monocytes / macrophages ( HMMs ) following treatment with interferon gamma ( IFNgamma ) , tumor necrosis factor alpha ( TNFalpha ) , and lipopolysaccharide ( LPS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Incubation of INS 1E cells with cytokines ( IL 1beta , TNF alpha and interferon gamma ; 10 50 ng / ml ) for 18 h significantly decreased cell viability ( by 44 % , p < 0 . 001 ) , cell proliferation ( by 80 % , p < 0 . 001 ) , and activation of PKB ( by 67 % , p < 0 . 001 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Since p 38 MAP kinase is involved in interferon gamma ( IFNgamma ) production by CD4+ T helper 1 ( Th 1 ) cells and a Th 1 immune response has been associated with RA , we investigated whether anti TNFalpha therapy could affect the activation of this signaling pathway in the CD4+ T cells of RA patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Apart from the white blood cells , intracellular T helper cell ( TH1 / TH2 ) cytokine production ( interferon gamma , IL 2 , IL 4 , IL 13 ) and HLA DR on monocytes were measured by four color flow cytometry , IL 6 , and TNF alpha with the fast immunoluminescence method . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increasing leptin levels from low physiologic to high physiologic in lean men and from higher physiologic to low pharmacologic in obese men over 3 d did not alter serum interferon gamma , IL 10 , TNF alpha , monocyte chemoattractant protein 1 , or soluble intercellular adhesion molecule 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The immune system is a system of dynamic equilibrium , with inflammatory responses ( mediated by T helper type 1 cells , interleukin [ IL ] 1beta , interferon gamma , and tumor necrosis factor alpha [ TNF alpha ] ) being balanced by anti inflammatory responses ( mediated by T regulatory type 1 cell , T helper type 3 cells , IL 4 , IL 10 , and transforming growth factor beta ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In cultivated human glioma cells as well as in activated mouse astroglial cells , CXCL 16 mRNA and protein is constitutively expressed and further up regulated by tumour necrosis factor alpha ( TNFalpha ) and interferon gamma ( IFNgamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CD4+CD25+CD27+ cells expressed high amounts of FoxP 3 ( 43 % of them being FoxP3+ ) , did not produce interleukin ( IL ) 2 , interferon gamma , or tumor necrosis factor , and suppressed T cell proliferation in vitro , being , on a per cell basis , fourfold more potent than the corresponding peripheral blood population . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , the capacity of both blood and peritoneal PMN to synthesize TNF alpha in vitro , in optimal conditions of stimulation ( lipopolysaccharide + interferon gamma ) , was impaired as compared with controls ' blood PMN . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
While in vitro experiments with Tanacetum parthenium found inhibition of the expression of intercellular adhesion molecule 1 , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma , IkappaB kinase , and a decrease in T cell adhesion , to date human studies have not proven it useful in the treatment of RA . ^^^ T . wilfordii Hook F extract inhibits the production of cytokines and other mediators from mononuclear phagocytes by blocking the up regulation of a number of proinflammatory genes , including TNF alpha , cyclooxygenase 2 ( COX 2 ) , interferon gamma , IL 2 , prostaglandin , and iNOS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Endotoxin ( LPS ) , interferon gamma ( IFNgamma ) , tumour necrosis factor ( TNF ) and TGF beta did not induce the S100A8 gene in murine fibroblasts whereas FGF 2 induced mRNA maximally after 12 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Despite normal basal expression of Toll like receptors and membrane CD 14 , innate immune responses of neonatal mononuclear cells to lipopolysaccharide are characterized by markedly reduced release of the pro inflammatory Th 1 polarizing cytokines TNF alpha and interferon gamma with relative preservation of anti inflammatory Th 2 polarizing cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNFalpha in combination with interferon gamma reduced progesterone ( P 4 ) secretion , increased PGF2alpha and leukotriene C 4 ( LTC 4 ) production , and induced apoptosis of the luteal cells in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Recent studies are focused on the effect and interaction between pro inflammatory ( interleukin 1 , interferon gamma and tumor necrosis factor ) and anti inflammatory cytokines ( interleukin 10 ) . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
By using such IL 1beta specific and TNF specific ELISA ' s , the respective cytokines were detected in supernatants of canine PBMC stimulated with LPS or heat killed Listeria monocytogenes and interferon gamma combined . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , various pro inflammatory cytokines , such as interleukin ( IL ) 1 , IL 6 , tumor necrosis factor alpha , and interferon gamma , produced at the site of inflammation activate neutrophils and suppress apoptotic death . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Macrophage products [ tumor necrosis factor a ( TNF a ) , interleukin 1ss ( IL 1ss ) , IL 6 , IL 12p40 ) ] , T lymphocyte products [ IL 2 , IL 4 , interferon g ( IFN g ) ] , and a proinflammatory chemokine ( IL 8 ) were assayed using ELISA in SF specimens from 53 patients with JIA [ ERA 34 , polyarticular RF negative 19 ] and 40 patients with rheumatoid arthritis ( RA ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The biological activity of the transferred TCR was shown by specific lysis of RCC cells ( ( 51 ) Cr release assay ) and by interferon gamma and tumor necrosis factor alpha release ( enzyme linked immunosorbent assay ) in an antigen specific and HLA A * 0201 restricted fashion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Our results have shown that , in the presence of interferon gamma , the Abeta 25 35 ( M ( 35 ) Nle ) peptide had almost the same potency of activating microglia and producing TNF alpha as the Abeta 25 35 peptide on both protein and mRNA levels , in spite of the fact that former peptide represented much less amyloid fibril formation than the latter in a thioflavine T fluorometric assay . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Changes in mean systemic blood pressure ( mSBP ) , mean pulmonary blood pressure , serum IL 6 , tumor necrosis factor alpha , interferon gamma , and highly mobile group 1 box protein were measured before CLP and at 1 , 3 , 6 , and 9 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor , interferon gamma , and interleukin 4 concentrations were significantly suppressed by ATL 146e treatment in supernatants from cultures of mesenteric lymph node cells of SAMP1 / YitFc mice ( P < . 05 vs vehicle treated mice ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Differential modulatory effects of cholera toxin and pertussis toxin on pain behavior induced by TNF alpha , interleukin 1beta and interferon gamma injected intrathecally . ^^^ Intrathecal injection of tumor necrosis factor alpha ( TNF alpha ; 100 pg ) , interleukin 1beta ( IL 1beta ; 100 pg ) and interferon gamma ( INF gamma ; 100 pg ) showed pain behavior . ^^^ Intrathecal pretreatment with CTX ( 0 . 05 , 0 . 1 and 0 . 5 mg ) attenuated pain behavior induced by TNF alpha and INF gamma administered intrathecally . ^^^ Furthermore , TNF alpha , IL 1beta and INF gamma administered spinally appear to produce pain behavior by different mechanisms . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Blood was sampled for estimation of endotoxins ( LPS ) , TNFalpha , interferon gamma ( IFNgamma ) , nitric oxide ( NO ) and malondialdehyde ( MDA ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After macrophages were stimulated by LPS+IFN gamma for 12 h or 24 h , the release of prostaglandin E 2 ( PGE 2 ) , nitric oxide ( NO ) , reactive oxygen species ( ROS ) , tumor necrosis factor alpha ( TNF alpha ) and interleukin 1beta ( IL 1beta ) from macrophages was measured by radioimmunoassay , Griess assay , fluorescence assay and enzyme linked immunosorbent assay , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To investigate the cytokine ( s ) involved in this arthritis , mice deficient in various arthritogenic cytokines , such as interleukin 6 ( IL 6 ) , IL 12 , IL 18 , interferon gamma , and tumor necrosis factor alpha ( TNFalpha ) , were sequentially treated with ECW and LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , the effect of LR on lipopolysaccharide ( LPS ) and recombinant interferon gamma ( rIFN gamma ) induced production of nitric oxide ( NO ) and tumor necrosis factor ( TNF ) alpha were examined using mouse peritoneal macrophages . ^^^ At 0 . 01 1 mg / ml , LR inhibited the LPS / rIFN gamma induced expression of inducible nitric oxide synthase ( iNOS ) and TNF alpha release . ^^^ These findings demonstrate that the inhibition of the LPS / rIFN gamma induced production of NO and TNF alpha by LR involves the inhibition of NF kappaB activation . . ^^^ Inhibition of lipopolysaccharide and interferon gamma induced expression of inducible nitric oxide synthase and tumor necrosis factor alpha by Lithospermi radix in mouse peritoneal macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The most important early response cytokines for innate immunity , tumour necrosis factor ( TNF ) alpha and interleukin ( IL ) 1beta , were tested in monocytes , and interferon gamma and IL 4 were studied as indicators of polarization of helper T cells into type 1 and type 2 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Th 1 cells and monocytes were expanded in the mesenteric nodes ( P < . 001 ) and blood ( P < . 001 ) of rats with cirrhosis , and activated to produce interferon gamma ( P < . 0001 ) and TNF alpha ( P < . 0001 ) , respectively . ^^^ Abrogation of bacterial translocation by bowel decontamination reduced the number of activated Th cells and monocytes , and normalized interferon gamma production by Th cells and TNF alpha production by monocytes in mesenteric nodes and blood , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The major immunomodulating effects of these active substances derived from mushrooms include mitogenicity and activation of immune effector cells , such as lymphocytes , macrophages , and natural killer cells , resulting in the production of cytokines , including interleukins ( ILs ) , tumor necrosis factor alpha ( TNF ) alpha , and interferon gamma ( INF ) gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A 549 human lung epithelial cells were treated with cytokines ( interleukin 6 , tumor necrosis factor alpha , interferon gamma , or interleukin 1beta ) or carbon particles ( CB and ufCB ) for 18 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Activation signals include cytokines ( interferon gamma , granulocyte monocyte colony stimulating factor , and tumor necrosis factor alpha ) , bacterial lipopolysaccharide , extracellular matrix proteins , and other chemical mediators . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These plasma samples were analyzed by the multiplex suspension bead array system ( Bio Rad Laboratories ; Hercules , CA ) , which included the following cytokines : interleukin ( IL ) 1beta , IL 2 , IL 4 , IL 5 , IL 6 , IL 7 , IL 8 , IL 10 , IL 12p70 , IL 13 , IL 17 , granulocyte / macrophage colony stimulating factor , interferon gamma , monocyte chemotactic protein 1 , macrophage inflammatory protein 1beta , tumor necrosis factor alpha , and granulocyte colony stimulating factor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 10 production was significantly increased , and interferon gamma and TNFalpha production was decreased in the spleens of Ucn 2 treated mice , compared with those in Ucn 2 untreated control mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
EXPERIMENTAL DESIGN : Eighteen consecutive breast cancer patients with bone metastases were evaluated for circulating levels of vascular endothelial growth factor ( VEGF ) , basic fibroblast growth factor ( bFGF ) , metalloproteinase 1 ( MMP 1 ) , metalloproteinase 2 ( MMP 2 ) , interleukins 1beta , 6 and 8 ( IL 1beta , IL 6 , IL 8 ) , interferon gamma , tumor necrosis factor alpha ( TNF alpha ) and transforming growth factor beta 1 just before and 2 and 7 days after ZA infusion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The levels of T lymphocyte subsets , interleukine 2 , 4 , 6 , 10 ( IL 2 , IL 4 , IL 6 , IL 10 ) , tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( INF gamma ) and Th1 / Th2 were determined before treatment , and at the end of 1st and 2nd week of treatment respectively . ^^^ RESULTS : Before treatment , levels of TNF alpha , IL 2 , IL 6 , IL 10 and INF gamma in all patients were significantly higher than normal range ( P < 0 . 05 ) . ^^^ After being treated for 1 week , the levels of serum TNF alpha , IL 6 , and IL 10 were significantly decreased in all groups ( P < 0 . 05 ) , serum IL 2 and INF gamma decreased to the normal level in the WM group , but in the treated and the FQG group by the end of the 2nd week , the two indexes still remained at the rather higher level ( P < 0 . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study examined whether the initial plasma levels of tumour necrosis factor alpha ( TNFalpha ) and interferon gamma ( IFNgamma ) in 131 children with newly diagnosed cancer were associated with haematopoietic suppression , and whether plasma levels of TNFalpha or haemoglobin at diagnosis affects long term prognosis in childhood acute lymphoblastic leukaemia ( ALL ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Unlike WT mice , PPARbeta KO mice showed a marked increase of prooxidant interferon gamma but no induction of nerve growth factor and tumor necrosis factor alpha after MCAO . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cells were pretreated with interferon gamma ( IFNgamma ) and / or lipopolysaccharide ( LPS ) followed byBSA ce 6 mal addition , and SRA expression , tumor necrosis factor alpha ( TNFalpha ) release , conjugate uptake and PDT killing were measured . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The hepatic lymphocytes are more Th 1 polarized with increased intracellular interferon gamma and tumor necrosis factor alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Intracolonic administration of oxazolone increased myeloperoxidase activity , damage score , and interleukin ( IL ) 4 , IL 10 , tumor necrosis factor alpha , and IL 1beta mRNA expression but lowered interferon gamma mRNA expression , indicating colonic inflammation of a TH 2 profile . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Apart from conventional antimycobacterial agents , novel therapeutic modalities , which stimulate the host immune system such as interleukin 2 ( IL 2 ) , IL 12 , interferon gamma , and tumor necrosis factor antagonists have been tested with promising results . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RDP 58 disrupts cellular responses signaled through the Toll like and tumor necrosis factor ( TNF ) receptor families and occludes important signal transduction pathways involved in inflammation , inhibiting the production of tumor necrosis factor alpha ( TNFalpha ) , interferon gamma , interleukin ( IL ) 2 , IL 6 , and IL 12 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Determinations included ( 1 ) tumor necrosis factor alpha ( TNF alpha ) interleukin ( IL ) 1beta , IL 6 , IL 10 , interferon gamma ; ( 2 ) myeloperoxidase ( MPO ) , superoxide anion ( SOA ) , superoxide dismutase ( SOD ) , and malondialdehyde ( MDA ) ; ( 3 ) nitrites ; ( 4 ) iNOS , ( 5 ) cell adhesion molecules ( ICAM 1 , VCAM 1 ) at 24 hours , 48 hours , and on day 7 ; and ( 6 ) NFkappaB at 48 hours . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MT 110 induced a costimulation independent polyclonal activation of CD 4 and CD 8 positive T cells as seen by de novo expression of CD 69 and CD 25 , and secretion of interferon gamma , tumor necrosis factor alpha , and interleukins 2 , 4 and 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Compared with the noninflammatory controls , HCV MC vasculitis patients had a significantly higher expression of Th 1 cytokines in vasculitic nerve lesions ( mean + / SEM fold increase 33 . 7 + / 11 . 6 for interferon gamma and 7 . 2 + / 1 . 9 for tumor necrosis factor alpha ) , whereas Th 2 cytokines were absent ( interleukin 4 [ IL 4 ] , IL 5 , and IL 13 ) or were not significantly different ( IL 10 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
No statistical differences were observed in the expression of interferon gamma , IL 1 beta , TNF alpha , and GM CSF among lepromatous , tuberculoid , and noninfected groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The NZBW strain lacked the FB 1 induced increases in the expression of liver tumor necrosis factor alpha , interferon gamma , receptor interacting protein ( RIP ) , and tumor necrosis factor alpha related apoptosis inducing ligand ( TRAIL ) , observed in CBL . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Four out of four patients who showed moderate or greater secretion of both interferon gamma ( IFNgamma ) and tumor necrosis factor alpha ( TNFalpha ) in response to a gp 100 antigen remained free of melanoma recurrence , whereas only two of six patients who showed discordant secretion of IFNgamma and TNFalpha did so . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum levels of tumor necrosis factor alpha ( TNF alpha ) , interferon gamma ( INF gamma ) and , interleutin ( IL ) 10 were collected at multiple time points , assayed by commercial enzyme linked immunosorbent assay , analyzed by analysis of variance . ^^^ Splenectomy drastically reduced INF gamma and TNF alpha levels compared to controls ( p < 0 . 05 ) on postoperative day 2 . ^^^ No method of spx preserved TNF alpha or INF gamma responses . ^^^ CONCLUSIONS : Splenectomy dramatically reduces TNF alpha and INF gamma responses to LPS challenge , although by different mechanisms . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The proinflammatory cytokines tumor necrosis factor alpha , interleukin ( IL ) 6 , IL 12 , and interferon gamma were upregulated to a significantly greater extent in apoE deficient mice than in wild type mice at both the mRNA and protein levels following administration of LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Plasma concentrations of tumour necrosis factor alpha ( TNFalpha ) , TNFalpha receptors TNFRI and TNFRII , interferon gamma ( IFNgamma ) , interleukin 2 ( IL 2 ) , IL 12 , IL 4 , and IL 10 were determined by enzyme linked immunosorbent assays . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The accumulated T cells express type 1 cytokines , such as interferon gamma ( IFNgamma ) and tumor necrosis factor alpha , and activated markers of inflammation , such as CD 154 and inducible costimulator ( ICOS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , some inflammatory cytokines and their downstream molecules were increased as well , including TNF alpha and its receptors , IL 6 signal transducer , and interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PD 1 blocked T cell migration in vivo , inhibited tumor necrosis factor alpha and interferon gamma secretion , and promoted apoptosis mediated by raft clustering . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The expression of chemokine receptors ( CCR 5 , CXCR 3 , CCR 4 ) and cytokines ( interferon gamma ( IFNgamma ) , interleukin ( IL ) 2 , IL 4 , IL 10 , tumour necrosis factor alpha ( TNFalpha ) ) on CD 28 ( + ) and CD 28 ( ) T cell subtypes was analysed by a three colour FACS technique of peripheral blood samples . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The induction of immunoproteasome subunits in G93A spinal cord organotypic slices treated with TNF alpha and interferon gamma suggest that certain cytokines may mediate such responses in vivo . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Studies on human subjects have generally reported decreased lymphocyte proliferation and decreased production of IL 2 , interferon gamma , IL 1beta , IL 6 and TNF alpha , but other studies showed no effect or even increased production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , a survey of cytokine production by H295R human adrenocortical cells demonstrated that these cells secreted IL 2 , IL 4 , IL 8 , IL 10 , IL 13 , and TNFalpha but not IL 5 , IL 12 , or interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To analyze the expression of adhesion molecules , eosinophils from healthy donors were cultured in the presence of interferon gamma and tumor necrosis factor alpha with various concentrations of fexofenadine , incubated with monoclonal antibodies anti ICAM 1 and LFA 1 and then analyzed by flow cytometry . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 2+ / mice presented with low level expression of cytokines ( IL 4 , IL 10 , interferon gamma , transforming growth factor beta ) , upregulation of tumor necrosis factor receptors , and increased leukocyte apoptosis susceptibility independent of AA expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human thyrocytes in primary culture produce large amounts of CXCL 10 when stimulated by interferon gamma ( IFNgamma ) and TNFalpha . ^^^ The effects of IFNgamma and TNFalpha stimulation and peroxisome proliferator activated receptor gamma ( PPARgamma ) activation on CXCL 10 secretion in primary cultures of thyrocytes , orbital fibroblasts , and preadipocytes were tested . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The activation and expansion of TNF producing T cells are key steps in estrogen deficiency driven bone loss and are regulated by multiple interacting cytokines including transforming growth factor beta , interleukin 7 , and interferon gamma , as well as by the process of antigen presentation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Paraffin sections were immunostained in triplicate for T cells ( CD 3 , CD 8 ) , macrophages ( CD 68 ) , and the cytokines tumour necrosis factor alpha ( TNFalpha ) , interferon gamma , interleukin ( IL ) 1beta , IL 6 , IL 10 , and transforming growth factor beta 1 ( TGFbeta 1 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , the expression of IL 15 receptor alpha ( IL 15Ralpha ) and membrane bound IL 15 was assessed and the in vitro effects of IL 15 on CD 69 and CD 64 expression , interferon gamma and TNF alpha synthesis , respiratory burst induction and apoptosis were studied . ^^^ Furthermore , diminished induction of CD 69 expression and interferon gamma and TNF alpha synthesis by recombinant human IL 15 was detected in peripheral blood mononuclear cells from SLE , and there was defective induction of CD 64 and priming for respiratory burst in neutrophils . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MCF 7 cells were stimulated with ( a ) E ( 2 ) ( 10 ( 8 ) M ) and ( b ) a combination of different cytokines such as interleukin 1 beta ( Il 1beta ) , tumor necrosis factor alpha ( TNF alpha ) and interferon gamma ( INF gamma ) , and lipopolysaccharide ( LPS ) . e NOS and 1 NOS proteins were measured using Western blot analysis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Regression of adipose tissue was accompanied by lymphohistiocytic infiltration / inflammation and increased serum levels of inflammatory cytokines interferon gamma and TNF alpha . ^^^ In vitro studies with human adipocytes demonstrated that interferon gamma and TNF alpha are able to up regulate CD 95 expression and enhance CD 95 death inducing signaling complex formation resulting in a robust sensitization for CD 95 mediated apoptosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , female mice treated with melatonin showed a diminution of titers of total serum IgG , IgM , and anti double stranded DNA and anti CII autoantibodies ; a decrease in proinflammatory cytokines ( IL 2 , IL 6 , interferon gamma , TNF alpha , and IL 1beta ) , an increase in antiinflammatory cytokines ( IL 10 ) , and a decrease in nitrite / nitrate . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Human saphenous vein smooth muscle cells , endothelial cells , and monocyte derived macrophages from 3 independent donors were stimulated with interleukin 1beta , interferon gamma , tumor necrosis factor alpha , basic fibroblast growth factor , and vascular endothelial growth factor , 5 common proinflammatory mediators often found in diseased human microvessels and macrovessels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TAK 242 inhibited mRNA expression of IL 6 and TNF alpha induced by LPS and interferon gamma in RAW264 . 7 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A cytometric bead array assay revealed that the level of tumor necrosis factor alpha ( Tnfa ) secreted by splenocytes increased significantly with increasing ( 56 ) Fe particle dose ( P < 0 . 05 ) ; interferon gamma , interleukin 2 ( Il 2 ) , Il 4 and Il 5 were unaffected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The release of cytokines : interleukin 2 ( IL 2 ) , IL 4 , IL 6 , IL 10 , interferon gamma ( INF gamma ) and tumor necrosis factor alpha ( TNF alpha ) was determined in supernatants of mononuclear cell cultures from 72 healthy subjects , measured by flow cytometry . ^^^ The release of INF gamma , IL 2 , IL 4 and TNF alpha in cultures from subjects with 2677 ( T T ) 3435 ( T T ) haplotype pair was significantly decreased as compared to subjects with other haplotypes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stress and SP boosted the abortion rate and increased the percentage of type 1 [ TNF alpha , interferon gamma , interleukin ( IL ) 12 ] and type 2 ( IL 4 and IL 10 ) cytokine producing lymphocytes in blood and decidua , predominantly CD8+ T cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
AIM : To study the cytokine spectrum ( IL 1beta , IL 2 , IL 4 , IL 6 , TNF alpha , interferon alpha , interferon gamma ) in patients with acute hepatitis C ( AHC ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The high mortality from sepsis is in part mediated by bacterial endotoxin , which stimulates macrophages / monocytes to sequentially release early ( e . g . , tumor necrosis factor , interleukin 1 , and interferon gamma ) and late [ e . g . , high mobility group box 1 protein ( HMGB 1 ) ] proinflammatory cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Similarly , percutaneous vaccination with Japanese BCG resulted in significantly greater secretion of the T helper 1 type cytokines interferon gamma , tumor necrosis factor alpha , and interleukin 2 ; significantly lower secretion of the T helper 2 type cytokine interleukin 4 ; and greater CD 4 ( + ) and CD 8 ( + ) T cell proliferation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PROBLEM : Considering that cytokines are involved in preeclampsia ( PE ) pathogenesis and that cytokine gene polymorphism may affect cytokine production , our purpose was to investigate the association of PE with tumor necrosis factor ( TNF ) alpha ( 308 ) , transforming growth factor beta 1 ( +10 ; 25 ) , interleukin ( IL ) 10 ( 1082 ) , IL 6 ( 174 ) , and interferon gamma ( +874 ) polymorphisms . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here , we extend our in vitro model to assess the individual and combined effects of HS loss , interferon gamma ( IFNgamma ) , TNFalpha , and increased pressure , and find that HS plays a central role in the patho mechanisms underlying PLE . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Some cytokines , including interleukin 2 and interferon gamma , were secreted into the synapse , whereas others , including tumor necrosis factor and the chemokine CCL 3 ( MIP 1alpha ) , were released multidirectionally . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Local cytokine profiles demonstrated that host resistance was characterized by enhanced synovial expression of tumor necrosis factor alpha , interferon gamma ( IFNgamma ) , and interleukin 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The heightened encephalitogenicity of NOS 2 / T cells correlated with enhanced expression of VLA 4 ( CD49d ) and increased production of interferon gamma and tumor necrosis factor . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1beta , IL 6 , IL 12 , IL 18 , TNFalpha , and interferon gamma are produced primarily in macrophages and have been associated with accelerated atherosclerosis and altered vascular wall function . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , these gliadin specific memory T cells are marked by a distinctive phenotype : They express high levels of the gut homing beta 7 integrins and primarily produce interferon gamma and tumor necrosis factor alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Nod 1 stimulation did not induce TNFalpha , interleukin 12 , and interferon gamma , suggesting that the primary role of Nod 1 is to induce the recruitment of immune cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We examined MDCK cell barrier function in response to challenge by the proinflammatory cytokines tumor necrosis factor alpha ( TNFalpha ) and interferon gamma ( IFNgamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found comparable levels of tumor necrosis factor ( TNF ) alpha , interferon gamma , interleukin ( IL ) 2 , IL 4 , IL 10 , and IL 12 in the sera of 22 patients with multifocal motor neuropathy ( MMN ) , 12 with amyotrophic lateral sclerosis ( ALS ) , 12 multiple sclerosis , seven chronic inflammatory demyelinating polyneuropathy , five myasthenia gravis , and 13 healthy controls ( NS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although the production of proinflammatory cytokines ( TNF alpha , interferon gamma , and interleukin 12 ) was reduced on the administration of the linear PEI polyplex , serum alanine aminotransferase levels were significantly enhanced by pDNA in a dose dependent manner , suggesting that such hepatic damage is not induced by proinflammatory cytokines . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma and neutralizing antibodies , but neither perforin nor TNF alpha alone are associated with clearance of MV infection . ^^^ TNF alpha combined with interferon gamma is more effective in protection than interferon alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : The angiotensin converting enzyme ( ACE ) insertion / deletion polymorphism and polymorphisms in the promoter regions of the genes for tumor necrosis factor alpha ( 308 A / G ) , interleukin 6 ( 174 G / C ) , and interferon gamma were examined for their association with rHuEPO requirements in 112 patients on continuous ambulatory peritoneal dialysis ( CAPD ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
An examination of lung cytokine levels in the context of active pulmonary infection revealed increased expression of interleukin ( IL ) 10 , tumor necrosis factor alpha , and IL 13 , but not IL 12 or interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum levels of tumor necrosis factor , interleukin ( IL ) 1beta , IL 12 and interferon gamma were evaluated . ^^^ The effects of tumor necrosis factor inhibitor ( thalidomide ) and anti interferon gamma antibody on infection outcomes were examined . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Herein we report that the repressible / inducible over expression of wild type SHP 1 in a subclone of RAW 264 . 7 macrophages ( RAW TT 10 cells ) inhibited both TNF secretion and iNOS protein accumulation in response to stimulation with lipopolysaccharide ( LPS ) and recombinant murine interferon gamma and led to diminished LPS mediated tyrosine phosphorylation of vav 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
T cell interferon gamma ( IFNgamma ) and macrophage tumour necrosis factor alpha ( TNFalpha ) activate chemokines such as , C C chemokine ligand 2 ( CCL 2 ) and CCL 5 , which play a role in granuloma formation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Heart infiltrating lymphocytes from patients with CCC also express proinflammatory cytokines ( tumor necrosis factor alpha and interferon gamma ) that are detectable in biopsy samples and surgical heart tissue samples . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The same procedures were carried out on filter grown Caco 2 cells incubated in the absence or presence of interferon g and tumor necrosis factor a . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We tested the effects of a range of injury related cytokines , of which tumor necrosis factor alpha ; interleukin 1 ; transforming growth factor beta ; interferon gamma had effects on oligodendrocyte precursor cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In a wide range of human diseases of inflammatory nature like Crohn ' s disease , pathology is mediated in part by pro inflammatory cytokines like tumor necrosis factor alpha ( TNF ) or interferon gamma . ^^^ We show here that a commonly used generic antidepressant bupropion , in wide use worldwide to treat depression in humans for a decade now , profoundly lowers levels of TNF , interferon gamma , and interleukin 1 beta in vivo , in a mouse lipopolysaccharide ( LPS ) induced inflammation model . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Second , infections in major histocompatibility complex ( MHC ) class 2 deficient mice indicate that this molecule is needed for initiation of IFN gamma and subsequent tumor necrosis factor ( TNF ) production . ^^^ Collectively , these results suggest that host protective immunity against T . congolense is critically dependent on the combined action of the proinflammatory mediators / effectors IFN gamma , TNF , and NO and antiparasite IgGs . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Isoflavone treatment did not significantly influence concentrations of interferon gamma , interleukin 2 , tumor necrosis factor alpha , or C reactive protein in plasma or of 8 isoprostane in urine . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Despite an activation of nuclear factor ( NF ) kappaB , NFinterleukin ( IL ) 6 and NFAT similar to LPS or TSST 1 , we observed no significant production of IL 1beta , IL 6 , tumor necrosis factor alpha or interferon gamma induced by glucan phosphate . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin 1beta ( IL 1beta ) , IL 10 , IL 16 , IL 17 , RANKL , tumor necrosis factor alpha ( TNFalpha ) , and interferon gamma ( IFNgamma ) messenger RNA ( mRNA ) expression was determined by quantitative reverse transcription polymerase chain reaction . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Platelet ( PLT ) derived ( CCL5 / RANTES , CXCL4 / PF4 , CCL3 / MIP 1alpha , transforming growth factor [ TGF ] beta , CXCL8 / interleukin [ IL ] 8 , IL 1beta ) as well as white blood cell ( WBC ) associated ( IL 6 , IL 10 , IL 11 , IL 12 , tumor necrosis factor , interferon gamma ) cytokines were investigated by enzyme linked immunosorbent assay and cytometric bead array during storage for up to 12 days . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After T cells were stimulated with CII , ovalbumin , and phytohemagglutinin , T cell proliferative responses and levels of cytokine production ( interferon gamma [ IFNgamma ] , tumor necrosis factor alpha [ TNFalpha ] , interleukin 10 [ IL 10 ] , and IL 17 ) were assayed in culture supernatants . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This increased IDR may be a consequence of the synergy between interferon gamma and tumor necrosis factor alpha which is a significant factor in diabetes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We first show that apigenin inhibits tumor necrosis factor alpha ( TNFalpha ) and interferon gamma ( IFNgamma ) induced secretion of several osteoclastogenic cytokines from MC3T3 E 1 mouse calvarial osteoblast cell line . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon gamma and TNF alpha potently induced TRAIL in IEC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CORM 3 ( 75 microM ) did not show any cytotoxicity and markedly attenuated the inflammatory response to thrombin and interferon gamma in normoxia and to a lesser extent in hypoxia as evidenced by a reduction in nitrite levels and TNF alpha production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Microglia cultured ex vivo from MK 2 deficient ( MK 2 / ) mice demonstrated significant inhibition in release of tumor necrosis factor alpha , KC ( mouse chemokine with highest sequence identity to human GROs and interleukin 8 ) , and macrophage inflammatory protein 1alpha on stimulation with LPS + interferon gamma or amyloid beta peptide ( 1 42 ) compared with MK2+ / + wild type microglia . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The J8R ( 11 19 ) specific CTL line and CTL clone exerted physiologically relevant functions as they recognized 10 infected lymphoblastoid JY cells or autologous B lymphoblastoid cell lines , and the cytolytic activity was accompanied by the production of interferon gamma , tumor necrosis factor alpha , and interleukin 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We developed a multiplexed immunoassay to simultaneously assess the plasma concentrations of 8 cytokines ( interferon gamma , interleukin ( IL ) 2 , IL 4 , IL 6 , IL 8 , IL 10 , IL 12 , and tumor necrosis factor alpha ) , IL 2r , and soluble CD 40 ligand in the patient groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A decrease in LPS induced tumor necrosis factor alpha ( TNF alpha ) , increased PHA induced interleukin 10 ( IL 10 ) release , and similar PHA induced interferon gamma production were observed in elderly subjects compared with young volunteers . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The abundance of transcripts encoding for interferon gamma ( IFNgamma ) , tumour necrosis factor alpha ( TNFalpha ) or Stat 1 did not differ significantly between both groups ( P > / = 0 . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Blood was drawn for complete blood count and serum concentration of the following cytokines : granulocyte colony stimulating factor , interleukins 6 , 8 and 10 , interferon gamma , tumor necrosis factor , as well as matrix metalloproteinase 9 and intercellular adhesion molecule 1 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
BACKGROUND : Mucosal leishmaniasis ( ML ) is associated with exaggerated tumor necrosis factor alpha and interferon gamma responses and tissue destruction . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : CD 1 mice were injected intraperitoneally with LPS ( 1 mg / kg ) , and the effects of pretreatment with a single intraperitoneal dose of telithromycin ( 150 mg / kg ) or roxithromycin ( 50 mg / kg ) for 2 h on the expression and formation of tumour necrosis factor alpha ( TNFalpha ) , interleukin 1 beta ( IL 1beta ) , interferon gamma ( IFNgamma ) and inducible nitric oxide synthase ( NOS 2 ) as well as nitric oxide ( NO ) were analysed at different time points after LPS treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lung homogenates were analyzed by enzyme linked immunosorbent assay ( ELISA ) for cytokines [ tumor necrosis factor alpha ( TNFalpha ) , interleukin ( IL ) 1beta , TGFbeta 1 , oncostatin M ( OSM ) , IL 10 , and interferon gamma ( IFNgamma ) ] and the chemokine MCP 1 and by Western blot for Smad 2 , Smad 3 , and Smad 7 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we purified the native MTB 12 protein and investigated the profile of MTB 12 induced cytokines [ interferon ( IFN ) gamma , tumour necrosis factor ( TNF ) alpha and interleukin ( IL ) 6 ] , in early tuberculosis ( TB ) patients ( n = 20 ) and healthy controls ( n = 35 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
ERalpha ligand treatment also induced favorable changes in autoantigen specific cytokine production in the peripheral immune system [ decreased TNFalpha , interferon gamma , and interleukin 6 , with increased interleukin 5 ] and decreased CNS white matter inflammation and demyelination . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Subsequent treatment with 1 % DSS for 7 d resulted in a more severe intestinal inflammation in SD / OC mice , as reflected by an increase in body weight loss , histological damage scores , and secretion of IL 6 , TNFalpha , and interferon gamma from mesenteric lymph node cells and by decreased colon length . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Correlation analyses revealed a negative association between salivary secretion and the levels of IL 4 , interferon gamma , and TNFalpha in saliva obtained from NOD mice , while the correlation with inflammatory changes in the glands was consistently weak . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Phytohemagglutinin P ( PHA P ) stimulated decidual CD 8 ( + ) T lymphocytes produced high levels of both interferon gamma and interleukin ( IL ) 8 , and low levels of granulocyte macrophage colony stimulating factor ( CSF 2 ) , IL1B , IL 2 , IL 6 , IL 10 , IL 12 , and tumor necrosis factor ; these did not vary with gestational age . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we evaluated intragraft mechanisms responsible for these effects by immunoperoxidase localization of relevant humoral mediators ( IgG , IgM , C 3 , cross linked fibrin ) , graft infiltrating cells ( GIC ) , and associated cytokines ( IL 2 , IFN g , tumor necrosis factor [ TNF ] , or cytokine receptors ( IL 2R ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytotoxicity of TNF and low dose actinomycin D ( AMD ) against MBT 2 in vitro was examined alone and following preincubation with IFNG . ^^^ A two hour preincubation with IFNG markedly increased the cytotoxicity of TNF / AMD whereas longer incubations did not enhance cytotoxic activity . ^^^ The in vitro data suggest brief exposure of MBT 2 cells to IFNG augments the subsequent anti tumor activity of TNF / AMD . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor increased IFN G induced MHC class 2 expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This report describes the development and application of real time RT PCR to measure the expression of several important elk ( Cervus elaphus ) cytokine mRNAs , including interleukin ( IL ) 2 , IL 4 , IL 10 , IL 12p40 , interferon gamma , tumor necrosis factor ( TNF ) alpha , and the enzyme inducible nitric oxide synthase , all of which are involved in immune responses and regulation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Two pancreatic beta cell lines , RINm5F and MIN 6 cells , were transfected with a rat insulin promoter ( RIP ) luciferase fusion gene and treated with a combination of cytokines , including 5 ng / mL interleukin 1beta + 10 ng / mL tumor necrosis factor alpha + 25 ng / mL interferon gamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The latter two genes may have a role in the increased permeability , so as the tumor necrosis factor alpha , interferon gamma may play affect it . ^^^ The tumor necrosis factor alpha , interferon gamma and lymphotoxin 3 alpha increased the chemokine secretion and adhesion molecule expression also . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Analysis of the pleural effusion revealed an increased CD4 / CD8 ratio because of an increased percentage of CD4+CD29+ helper memory T cells , lack of expression of the resting B cell marker CD 21 , immune complex deposition and complement consumption , increased relative levels of ANA , abnormally high levels of IL 6 and soluble IL 2R , and detectable levels of IL 1b , IFN g and TNF a . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines assessed in this study included interleukin 1 , IL 1 , and tumor necrosis factor ( TNF ) alpha produced by macrophages , and interleukin 2 , IL 2 , and gamma interferon ( IFN G ) secreted by T lymphocytes . ^^^ Activities of TNF and IFN G include antiviral properties and induction of expression of class 1 and 2 major histocompatibility complex molecules , which are critical components in the recognition of antigen by T lymphocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF , IFNg , and GMCSF , to activate neutrophil function against C . albicans . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 2 , IFNgamma , lymphotoxin , and tumor necrosis factor ( TNF ) are made by activated Th 1 type T cells , IL 1 , and TNF by activated macrophages . ^^^ IFNbeta1b increases IL 2 production in vitro but decreases production of IFNgamma , LT , and TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Intragraft cytokine levels of TNFalpha , and IFNgamma were determined using ELISA and quantitative PCR . ^^^ Enhanced production of TGF beta 1 in transduced allografts was associated with decreased levels of TNFalpha and IFNgamma when compared with nonimmunosuppressed controls . ^^^ Transduction of allografts with TGF beta 1 down regulates TNFalpha and IFNgamma production early after orthotopic transplantation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunohistological evaluation of renal allografts revealed that therapy with ART 18 or low dose CsA alone failed to deplete IL 2R+ cells and prevent production of IL 2 , IFN g , and TNF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study details the use of a monovalent anti CD 3 monoclonal antibody in the treatment of allograft rejection in five renal transplant recipients and documents the degree of TNF , IFN g and IL 6 release generated after antibody injection . ^^^ TNF , IFN g and IL 6 measurement showed that little pro inflammatory cytokine release occurred after this drug . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The effects of the anti picornaviral drug WIN 54954 ( 5 ( 5 ( 2 . 6 dichloro 4 ( 4 . 5 dihydro 2 oxazolyl ) phenoxy ) pentyl ) 3 me thyl isoxazole ) and the immune modulator LS 2616 ( Quinoline 3 carboxamide ) on plasma cachectin / TNFa and g interferon ( IFN g ) responses were investigated during the clinical course of a myocarditic coxsackievirus B 3 ( CB 3 ) infection in the mouse . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We now show that DHA inhibits production of nitric oxide ( NO ) by macrophages stimulated in vitro by IFNgamma plus LPS , or by IFNgamma plus TNFalpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The specificity of our ELISA was confirmed by the lack of cross reactivity with other cytokines , including IFNalpha , IFNgamma , IFN Consensus 1 , and TNFalpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
By contrast , DEX inhibited XS 52 DC stimulated IL 2 secretion by HDK 1 T cells , but not other changes that accompany T cell activation , including the secretion of IFNgamma and TNFalpha and the elevated expression of CD 25 , CD 28 , and CD 44 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , the increase in IL 2 serum concentration appeared selective in this CD56+ large cell lymphoma bearing patient , because concentration of none of the five other cytokines tested ( IL 4 , IL 6 , IFNgamma , GM CSF , and TNFalpha ) was increased . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , this does not exclude persistent increased intracellular deposition of microbial antigens as a possible explanation for the elevated incidence of IL 1beta , TNF alpha , IL 6 , IL 8 , IL 2 , IFNgamma , IL 10 and IL 4 expressing cells noticed in patients with recurrent tonsillitis . . ^^^ Cytokine producing cells ( IL 1alpha , IL 1beta , TNFalpha , IL 6 , IL 8 , IL 2 , IFNgamma , TNFbeta , IL 10 and IL 4 ) were characterized at the single cell level by use of cytokine specific monoclonal antibodies and indirect immunofluorescence technique . ^^^ However , the frequency of spontaneous IL 2 , IFNgamma , TNFalpha , IL 6 and IL 10 was consistently low ( 10 + / 10 cells ) in both groups . ^^^ Following restimulation by T cell receptor ligation , using immobilized anti CD 3 mAb , with concentrations chosen so that it did not activate resting cells , increased frequencies of TNFalpha , IL 6 , IL 8 , IL 2 , IFNgamma , IL 4 and 1L 10 synthesizing cells were induced in the recurrent tonsillitis group . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
C 3 production of cultured human epidermal keratinocytes is enhanced by IFNgamma and TNFalpha through different pathways . ^^^ The results showed that IFNgamma and TNFalpha enhanced the synthesis of C 3 by epidermal keratinocytes in a concentration dependent manner . ^^^ There was a synergistic effect between IFNgamma and TNFalpha . ^^^ In experiments to investigate the role of protein tyrosine kinase ( PTK ) in C 3 production , we found that treatment with herbimycin A , a specific inhibitor for the c Src related PTK , caused significant enhancement of the C 3 production induced by IFNgamma or TNFalpha , suggesting that c Src type PTK ( s ) provides a negative signal to C 3 production . ^^^ Each competitive inhibitor of PTK , genistein or tyrphostin , substantially increased the C 3 production by IFNgamma at lower concentrations , although each agent had little effect on TNFalpha associated production of C 3 at the same concentrations . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The administration of the immunosuppressive humanized monoclonal antibody CAMPATH 1 H , which recognizes CD 52 on lymphocytes and monocytes , is associated with a first dose cytokine release syndrome involving TNFalpha , IFNgamma , and IL 6 clinically . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lung lavage levels of TNFalpha , IL 1beta , IL 6 , IL 10 , MIP 2 , and IFNgamma were measured by ELISA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The unstimulated cytokine profile in these patients generally showed marked elevations in IL 1 , IL 3 , IL 4 , IFN G , TNF A , TNF B , OSM , and TGF B . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Dedifferentiated human ventricular cardiac myocytes express inducible nitric oxide synthase mRNA but not protein in response to IL 1 , TNF , IFNgamma , and LPS . ^^^ Therefore , we treated cultured , dedifferentiated human ventricular cardiac myocytes with the combination of TNF alpha ( 500 U / ml ) , IL 1beta ( 30U / ml ) , IFNgamma ( 100 U / ml ) , and LPS ( E . coli 0111 : B 4 , 10 microg / ml ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Th 1 products ( IL 2 , TNFalpha , IFNgamma ) and macrophage associated products ( IL 1 , IL 6 , TGFbeta ) remained highly expressed after 2 d . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A significant induction of guanosine triphosphate cyclohydrolase 1 ( GTPCH ) mRNA was observed in response to TNF , IL 1beta , and IFNgamma in ECV and HUVEC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here , we show that tumor necrosis factor receptor p 55 / ( TRp 55 / ) mice are susceptible to Listeria monocytogenes infection in the presence of leukocyte recruitment , inflammatory cytokine production ( including IFNgamma ) , nitric oxide synthesis , and oxidative burst formation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Systemically , intraperitoneal delivery of endotoxin resulted in much more pronounced circulating levels of TNFalpha , MIP 2 , GM CSF , and IFNgamma in IL 6 / mice than in IL 6+ / + mice , and administration of recombinant IL 6 to IL 6 / mice abolished these differences . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin 18 ( IFNgamma inducing factor ) induces IL 8 and IL 1beta via TNFalpha production from non CD14+ human blood mononuclear cells . ^^^ In the presence of IFNgamma , IL 18 induced TNFalpha was enhanced and there was an increase in the mature form of IL 1beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , gammadelta T cell clones responded to stimulation by several cytokines including IL 12 , IFNgamma and TNFalpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Analyses of cytokine mRNA expression in the submandibular glands demonstrated an increase between 12 and 16 wk of age of several proinflammatory cytokines including IL 1beta , IL 6 , IL 7 , IL 10 , IFNgamma , TNFalpha , and inducible Nitric Oxide Synthase ( iNOS ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We have successfully developed a method for the immunohistochemical detection of interleukin 2 ( IL 2 ) , IL 4 , IL 6 , IL 10O , IFNgamma and TNFalpha using monoclonal antibodies ( MAb ) , in sections of mouse tissue embedded in paraffin wax . ^^^ The following tissues were shown to be suitable as positive controls when using paraffin embedding : spleen for the detection of TNFalpha , small intestine for IL 2 , IL 4 and IL 10 , and HSV 1 infected eyes for IL 6 and IFNgamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Changes in IFNgamma , IL 8 , IL 10 , MCP 1 and TNF alpha mRNA levels in peripheral blood cells were also studied 4 h and 24 h after the first G CSF injection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Concerning PBMC , the production of TNF alpha and IL 12 in response to LPS , or LPS plus IFNgamma , was found to be significantly higher in cells isolated from HIV positive patients , whereas the release of IL 1beta was significantly lower . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Stimulation of the IGM 36 cells with TNF alpha and IFNgamma resulted in increased expression of B 7 1 , class 1 MHC and class 2 MHC and consequent presentation of antigen in allogeneic MLRs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using these monocyte derived DCs , we examined the ability of various cytokines , such as IL 1beta , IL 3 , IL 5 , IL 6 , IL 7 , IL 8 , IL 10 , TNFalpha , TGFbeta , M CSF , IFNalpha , and IFNgamma , and phorbol 12 myristate 13 acetate ( PMA ) , to induce different cell types observed in DFs . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of CD 44 variants was unaffected by IL 1beta , IL 8 , TNFalpha , IFNgamma or IL 4 treatment , indicating either constitutive expression of these variants or involvement of other cytokines in their regulation . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
When HUVSMC were activated by individual inflammatory stimuli ( IL 1beta , TNFalpha , IFNgamma or LPS ) , both intra and extracellular levels of BH 4 increased significantly , with TNFalpha being the most potent single stimulus . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Studies showed that beta 1 , 3 glucan , IL beta , TNFalpha and IFNgamma / TNFalpha induced expression and production of CINC in macrophages while neither IFNgamma nor TGFbeta alone induced detectable CINC expression . ^^^ These studies demonstrate that IFNgamma and TNFalpha , found early during the inflammatory response , induce production of CINC , as well as induce macrophages into a cytocidal state that are capable of killing transformed cells , parasites and bacteria , and recruiting neutrophils . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine induced expression of ICAM 1 , VCAM 1 , and MHC class 1 and 2 was studied at different time points in microvascular endothelial cells ( EC ) of heart origin , using three different rat endothelial cell ( RHEC ) lines that were stimulated with TNFalpha and / or IFNgamma . ^^^ Each of the three RHEC lines responded to TNFalpha as well as to IFNgamma ; stimulation with combined cytokines led to increased or even synergistic effects . ^^^ TNFalpha was most potent in inducing ICAM 1 and VCAM 1 , whereas MHC class 2 was most effectively induced by IFNgamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) , which alone had no effect , synergistically enhanced this effect of IFNgamma . ^^^ Tumor necrosis factor alpha ( TNF alpha ) , which alone had no effect , synergistically enhanced this effect of IFNgamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , mRNA expression of two inflammatory cytokines , TNFalpha and IFNgamma , decreased following steroid therapy . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of myoblasts with TNFalpha or IFNgamma led to the expression of a range of immunostimulatory molecules including MHC class 1 and 2 , and CD 95 ( Fas ) , but not B 7 family molecules . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We used a flow cytometric cytotoxicity test to determine the efficacy of the cytokines interferon alpha ( IFNalpha ) and gamma ( IFNgamma ) , interleukin 2 ( IL 2 ) , macrophage colony stimulating factor ( M CSF ) , granulocyte / macrophage CSF ( GM CSF ) and tumor necrosis factor alpha ( TNFalpha ) in enhancing the antibody dependent cellular cytoxicity ( ADCC ) of the mAb 17 1A and the mAb BR 55 2 against the colorectal carcinoma cell line HT 29 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Induction of human immunodeficiency virus type 1 replication in human glial cells after proinflammatory cytokines stimulation : effect of IFNgamma , IL1beta , and TNFalpha on differentiation and chemokine production in glial cells . ^^^ We observed that HIV replication was induced when CNS cell cultures were stimulated for 14 days by a combination of proinflammatory cytokines including IFNgamma , IL1beta , and TNFalpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of genes coding for the pro inflammatory TNFalpha , IL 1beta and cellular response cytokines IFNgamma and IL 2 was detected beginning with the second and third week after infection in all BLV infected animals . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
INTRODUCTION : Isolated limb perfusion ( ILP ) with TNFalpha in combination with melphalan and IFNgamma has resulted in an immediate and dramatic tumor response in patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Oligonucleotide target probe sets were designed for several human cytokines , including TNFalpha , IL 2 , IL 4 , IL 6 , IL 10 , and IFNgamma . ^^^ Elevated levels of IFNgamma mRNA were detected in PBMCs from HIV 1 seropositive individuals , but no differences in mean levels of TNFalpha or IL 6 mRNA were detected between seropositive and seronegative individuals . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To circumvent this problem , we developed an alternative procedure which allowed us to show that in neutrophils , LPS and TNFalpha induce a NF kappaB DNA binding activity which essentially consists of p50 / RelA dimers , and that IFNgamma promotes the binding of STAT 1 homodimers to the IFNgamma response region of the FcgammaRI promoter . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , TNFalpha and IFNgamma moderately increased TN C expression . ^^^ The regulation of TN C expression in keratinocytes is distinct from that of fibronectin , since IL 4 and IFNgamma did not affect fibronectin expression in our experiments , and TNFalpha only slightly increased fibronectin levels . ^^^ TN C expression by squamous carcinoma cell lines was differentially affected by the cytokines that stimulated TN C expression in normal keratinocytes : TNFalpha again increased TN C secretion , but IL 4 and IFNgamma had little effect . ^^^ The observation that TN C is abundant in inflamed skin is a strong indication that inflammatory cytokines such as IL 4 , TNFalpha and IFNgamma could also be involved in the regulation of epidermal TN C expression in vivo . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Patients of groups A 1 and A 2 had spontaneously higher tumor necrosis factor alpha ( TNF alpha ) and lower IL 12 and IFNgamma production compared to patients from groups B 1 and B 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine release ( IL 1beta , TNFalpha , IL 6 , GM CSF and IFNgamma ) was assessed on both PBMCs cultured with AIM 5 only ( unstimulated PBMC ) and PBMCs cultured with AIM 5 plus phytohaemagglutinina ( PHA stimulated PBMC ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , we found that TNF alpha is able to enhance the IFNgamma mediated anti microbial effect . ^^^ We found that the IFNgamma or the IFNgamma / TNF alpha induced activation of indoleamine 2 , 3 dioxygenase is responsible for the inhibition of streptococcal growth , since the addition of supplemental L tryptophan completely blocks the IFNgamma induced bacteriostasis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition to CD 4 , islet mRNA from these mice also showed expression of CD 8 , IFNgamma , TNFalpha , Fas , and Fas ligand . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The production of IFN g , IL 2 , TNF a ( products of TH 1 cells ) were decreased , whereas the production of IL 4 , IL 6 and IL 10 ( products of TH 2 ) were not affected during zinc deficiency . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
As expected , IFNgamma and TNFalpha upregulated expression of ICAM 1 , MHC class 1 and MHC class 2 , particularly in biliary epithelial cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TGF beta , TNFalpha and IL 1beta , but not IFNgamma , induced MCP 1 mRNA and protein . ^^^ Treatment of astrocytes with TGF beta prior to TNFalpha , IFNgamma or IL 1beta treatment significantly increased MCP 1 expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The results showed that the cell lines transcribed message for IFNgamma , TNFalpha , IL 4 and IL 10 whereas no mRNA for IL 2 or IL 1beta was detected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Enhancement of MHC class 2 antigen expression by islets using TNFalpha and IFNgamma did not increase their stimulatory capacity in the islet cocultures , which may have been due to a corresponding absence of B 7 expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast to IL 1beta , TNFalpha and IFNgamma , which are ineffective in inducing COX 2 and releasing PGE 2 from human ASM cells , did not affect the cyclic AMP formation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interestingly , significantly increased TNFalpha and IFNgamma mRNA levels were observed among clones derived from HLA DR 2 positive versus HLA DR 2 negative MS patients . ^^^ The high levels of TNFalpha and IFNgamma mRNA observed in MBP reactive T cell clones from MS patients indicate an important role of these cytokines in the disease process . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The combined detection of these surface antigens with intracellular molecules , including Bcl 2 and cytokines ( IFNgamma , TNFalpha , IL 2 ) was also analysed in the context of these three permeabilizing procedures . ^^^ We report that permeabilization with saponin is the only procedure which allows : ( 1 ) the preservation of lymphocyte morphology determined by the FSC / SSC parameters ; ( 2 ) the quantification of apoptotic lymphocytes following 7 AAD staining ; ( 3 ) a reliable surface immunophenotyping , maintaining a good antibody binding capacity ( ABC ) ; ( 4 ) the proper detection of intracellular membrane bound antigens ( Bcl 2 ) and intracellular cytokines ( IFNgamma , TNFalpha , IL 2 ) ; ( 5 ) the combined detection of apoptotic nuclei , surface antigens and intracellular molecules . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the acute phase of disease , perivascular immune cell infiltrates consisted of CD 4 + and CD 8 + T cells , macrophages and NK cells with peak expression of mRNAs encoding the cytokines IL1alpha , IL 2 , IL 6 , TNFalpha , and IFNgamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , high levels of NK and LAK activities and significant increases of the numbers of cells positive for asialoGM 1 , IFNgamma , TNFalpha , or IL 1beta were detected in the spleen cells derived from animals given cisplatin or 5 FU as compared with those given saline ( P < 0 . 001 0 . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFNgamma caused a significant dose dependent inhibition in the TNFalpha stimulated expression of MMP 9 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Dose response and kinetics were investigated for the production of TNFalpha , IL 1beta , IL 1ra , IL 10 and IFNgamma . ^^^ LPS induces the production of TNFalpha , IL 1beta , IL 1ra and IL 10 ; PHA induces the production of IFNgamma and IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Western blot analyses identified no cross reactions of the purified PIF with TGF alpha , TNFalpha , IFNgamma , or IL 1beta , suggesting that PIF is a new protein belonging to the group of factors secreted by fibroblasts able to inhibit cellular proliferation . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Ribavirin enhanced a Type 1 ( IL 2 , IFNgamma , TNFalpha ) while suppressing a Type 2 cytokine response ( IL 4 , IL 5 and IL 10 ) , at the level of both protein and mRNA expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A high percentage of CD8+ lymphocytes from 4 / 5 HPS+ patients produced TNF alpha and IFNgamma , but no IL 6 upon in vitro activation . ^^^ Although other cytokines might be involved in the pathophysiology of HPS as suggested by the high expression of M CSF receptor , these results suggest that TNF alpha and IFNgamma secretion by T cells might play a role in the up regulation of adhesion and MHC molecules on monomacrophages from HPS . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The three cell lines display common but also specific proliferative responses to cytokines ( IL1beta , IL 2 , IL 8 , TNFalpha , IFNgamma , TGFbeta 1 , and HGF ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We observed in the sera of 20 mer peptide treated mice that the concentrations of IFNgamma , TNFalpha and IL1alpha increase to a peak level by 4 h ; on the other hand , there was a decrease in IL 4 , IL 6 and IL 10 concentrations at the same time ( 4 h ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Comparison of cell culture releasing amounts of IL 2 , IL 10 , TNFalpha , IFNgamma , MIP 1alpha , MCP 1 , and RANTES demonstrated that MIP 1alpha had increased levels when NO production was blocked with L NAME , whereas IL 10 secretion decreased in presence of L NAME . ^^^ The other tested cytokines ( IL 2 , TNFalpha , and IFNgamma ) and chemokines ( MCP 1 and RANTES ) showed no significant differences between the presence or absence of L NAME . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interferon beta 1b treatment modulates TNFalpha and IFNgamma spontaneous gene expression in MS . ^^^ OBJECTIVES : To determine whether IFNbeta 1b treatment had an initial and sustained effect on the in vivo synthesis and secretion of tumor necrosis factor alpha ( TNFalpha ) and IFNgamma . ^^^ Initially , patients ' peripheral mononuclear cells secreted diminished amounts of TNFalpha and IFNgamma on PHA + PMA mitogen stimulation in comparison with normal control subjects . ^^^ After 1 year of therapy , IFNbeta 1b restored the normal production of TNFalpha , whereas therapy did not restore IFNgamma secretion to control values . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Peripheral nerve fibroblasts as a source of IL 6 , TNFalpha and IL 1 and their modulation by IFNgamma . ^^^ All MA 1 cells expressed mRNA for IL 6 , TNFalpha , and IL 1 plus or minus IFNgamma stimulation . ^^^ IFNgamma stimulation significantly reduced the production of IL 6 but increased TNFalpha production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We also observed a novel injury dependent switch from CD4+ to CD8+ T cells as the dominant T cell type producing TNFalpha and IFNgamma in response to SEA stimulation in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Secretion of cytokines to the culture medium was measured either by biological assay ( IL 2 ) or by ELISA ( IL 4 , IL 10 , IFNgamma , TNFalpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Increased IL 4 and TGF beta and decreased IL 2 , IFNgamma , and TNF alpha mRNA expression by PBMCs in response to donor alloantigen stimulation predicted immunologic graft stability over a minimum 60 day interval compared with mRNA expression of PBMCs from patients with established rejection or those who experienced a rejection episode within a 30 day period ( p < 0 . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immunohistochemical staining showed that the infiltrating lymphocytes displayed TNFalpha , TNF type 1 receptor and a variety of cytokines including IL 1beta , IL 4 , IL 6 , IL 10 , IFNgamma or IL 12 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Blood samples were taken for total white cell count , PMNs , TNFalpha and IFNgamma . ^^^ TNFalpha ( P < 0 . 05 ) and IFNgamma ( P < 0 . 001 ) levels were significantly increased in the burn + vehicle animals compared to the burn + GCSF animals . ^^^ GCSF modifies the immune system , as shown by an increase in white cell and PMN counts and by balancing the overall immune response from proinflammatory to normal , as shown by decreased TNFalpha and IFNgamma levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Initial rise of all splenic cytokines and serum anti SEA IgE levels at 6 weeks p . i . was observed , followed by a dramatic drop in the levels of the proinflammatory cytokines IL 2 , IFNgamma , IL 4 and TNF alpha and IgE at 8 weeks of infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNFalpha alone , and more potently in combination with IFNgamma , induced a high degree of IEC 6 cell apoptosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We determined effects of IL 1alpha , TNFalpha and IFNgamma on sICAM 1 release in culture media from human aortic smooth muscle cells ( AOSMC ) , dermal microvascular endothelial cells ( DMEC ) , keratinocytes ( KC ) , bronchial epithelial cells ( BEC ) and lung fibroblasts ( LF ) as determined by ELISA . ^^^ IL 1alpha , TNFalpha and IFNgamma stimulated sICAM 1 from these cells . ^^^ IFNgamma stimulated more shedding from AOSMC , BEC and KC than IL 1alpha or TNFalpha . ^^^ IL 1alpha and IFNgamma or TNFalpha and IFNgamma acted synergistically to enhance shedding of sICAM 1 from these cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , the cytotoxic activation of beta glucan primed NK cell CR 3 by iC3b opsonized tumors is shown to be accompanied by a tumor localized secretion of the cytokines TNFalpha , IFNalpha , IFNgamma , and IL 6 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Viral load was quantified by in situ hybridisation , CD 4 and CD 8 T cell infiltration by immunohistochemistry and mRNA cytokine expression ( IL1beta , IL 2 , IL 6 , TNFalpha , IFNgamma and TGF beta 1 ) by semiquantitative RT PCR . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Reverse transcriptase polymerase chain reaction ( RT PCR ) amplification of tumor necrosis factor ( TNFalpha ) , IFNgamma , IL 6 and BCGF was equally often seen in non progressive and progressive patients . ^^^ TNFalpha and IL 10 were also detected in culture supernatants from in vitro stimulated B CLL cells , whereas IFNgamma was undetectable in these cultures and rarely positive in serum . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CD 40 expression significantly increased after 24 hours of IFNgamma treatment and after 48 hours ' exposure to TNFalpha . ^^^ HLA DR was upregulated after 24 hours of treatment with IFNgamma but remained negative after exposure to TNFalpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The targeting of Th 1 cells to CMS7HE tumor cells by bsAb also triggered the production of cytokines such as IFNgamma , interleukin 2 and tumor necrosis factor alpha ( TNFalpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Hematopoietic progenitor cells from patients and mice with FANCC mutations are hypersensitive to the inhibitory effects of IFNgamma and TNFalpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Incubation of human Schwann cells with TNFalpha , IFNgamma and IL 1beta induces the expression of ICAM 1 starting at 6 h and reaching a peak at 24 h on more than 90 % of cells . ^^^ Surprisingly , stimulation with TNFalpha , IFNgamma and IL 1beta also induced the expression of L selectin on fetal and diabetic Schwann cells , but not on normal adult cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both IL 4 and IL 13 significantly inhibited TNF induced IL 6 release ( P < 0 . 01 for both ) while augmenting the effect of IFNgamma ( P < 0 . 005 and P < 0 . 01 , respectively . ) . ^^^ IL 4 caused a slight but significant increase in IL 6 release ( 530+ / 45 pg / ml ) , P < 0 . 05 , TNFalpha ( 1657+ / 85 pg / ml ) and IFNgamma ( 1953+ / 37 pg / ml ) showed strong induction of IL 6 release in HBECs ( P < 0 . 005 and P < 0 . 001 , respectively ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A ' cytokine storm ' consisting of IL 1 , IL 2 , IL 12 , IFNgamma and TNFalpha is considered important in the development of graft versus host disease ( GvHD ) . ^^^ The cytokine profile ( IL 1alpha / beta , IL 2 , IL 4 , IL 6 , IL 8 , IL 10 , IL 12 , IFNgamma and TNFalpha ) of cord blood cells induced by immune stimuli was determined in heparinized whole blood . ^^^ Compared to adult , cord blood CD3+ and NK cells produced less IFNgamma , less cord blood CD3+ cells and monocytes produced TNFalpha and less monocytes produced IL 1alpha / beta . ^^^ Both adult and cord mononuclear cells constitutively expressed receptors for IFNgamma and TNFalpha but not IL 12 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The production of IFNgamma , IL 1beta , and TNFalpha was almost completely abrogated in the microgravity cultures , suggesting that the IL 2 signaling pathways leading to various IL 2 mediated effects are differentially regulated under bioreactor culture conditions . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pretreatment of rRVE with IFNgamma , TNF or IL 1beta resulted in a significant decrease in T . gondii replication from day 2 onwards . ^^^ There was no significant difference in nitric oxide ( NO ) production by IFNgamma , TNF or IL 1beta treated rRVE as compared to controls at any time point . ^^^ Thus , IFNgamma , TNF and IL 1beta can significantly inhibit the replication of T . gondii within rRVE . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
By using RNase protection assays and RT PCR , we have systematically studied IL1alpha , IL1beta , IL 1 Ra , IL 2 , IL 4 , IL 5 , IL 6 , IL 9 , IL 10 , IL12p35 , IL12p40 , IL 13 , IL 14 , IL 15 , IFNgamma , IFNbeta , TNFalpha , TNFbeta , LTbeta , and TGFbeta 1 , TGFbeta 2 and TGFbeta 3 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Stimulation of A 549 cells with TNFalpha , IFNgamma or a combination of 50 ng / mL of TNFalpha , IFNgamma and IL 1 ( cytomix ) did not effect eosinophil binding despite an increase in ICAM 1 expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These effects are mediated by gamma interferon ( IFNgamma ) , tumor necrosis factor alpha ( TNFalpha ) , and IFNalpha / beta . ^^^ In the present study , we crossed HBV transgenic mice with mice genetically deficient for IFNgamma ( IFNgammaKO ) , the TNFalpha receptor ( TNFalphaRKO ) , or the IFNalpha / beta receptor ( IFNalpha / betaRKO ) in order to determine the relative contribution of each cytokine to the antiviral effects observed in each of these systems . ^^^ We also compared the relative sensitivity of LCMV and adenovirus to control by IFNgamma , TNFalpha , or IFNalpha / beta in these animals . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The number of cardiac cells displaying intracellular amastigotes was lower in cultures supplemented with IL 1b , TNF a and IFN g than with other cytokine combinations and controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Serum concentrations of several acute phase proteins ( C reactive protein , serum amyloid A ) , proinflammatory cytokines ( TNFalpha , IL 1 , IFNgamma , IL 6 , IL 8 ) , soluble adhesion molecules ( soluble E selectin , vascular cell adhesion molecule 1 , intercellular adhesion molecule 1 ) and more recently procalcitonin have been investigated as to whether these may contribute to identifying infections as the cause of neutropenic fever . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These cells produce the T helper 1 type ( Th 1 ) cytokines IFNgamma and TNFalpha , but not the T helper 2 type ( Th 2 ) cytokine IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The results showed that in human microglia , IP 10 expression was induced equally potently by LPS , IFNbeta or IFNgamma . `` Proinflammatory ' ' cytokines IL 1beta or TNFalpha also induced small amounts of IP 10 mRNA . `` Anti inflammatory ' ' cytokines IL 4 , IL 10 and TGFbeta were ineffective in inducing IP 10 in microglia . ^^^ IFNbeta but not IFNgamma was effective in inducing the expression of beta chemokines MIP 1alpha and MIP 1beta in human microglia , with the levels of mRNA similar to those induced by IL 1beta or TNFalpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Early increased production of pro inflammatory cytokines ( TNFalpha and IL 1 ) , followed by a peak of the Th 1 type cytokines ( IL 2 , IFNgamma ) were observed in young mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytotoxic T lymphocyte ( CTL ) activity , IFNgamma and tumor necrosis factor production of the splenocytes increased significantly in mice after intravenous injection of the gene modified macrophages . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The kinetics of TNFalpha downregulation 5 d after lesion was not affected by transgenic IFNgamma , indicating that IFNgamma acts as an amplifier and not an inducer of response . ^^^ These results are discussed in the context of a regenerative role for TNFalpha in the CNS , which is innately regulated and potentiated by IFNgamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNFalpha and IFNgamma did not significantly influence the expression of CD 29 or CD 44 , and no alteration in tumor cell migration was observed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These involved ( a ) increased plasma levels of neopterin and sTNF receptor indicating elevated IFNgamma and TNF cytokine activity ; ( b ) increased proportion of mature ( CD45RO ) versus nave ( CD45RA ) T cells ; and ( c ) a diverse group of related changes including impaired proliferative response , reduced T cells , CD 28 and CD 25 expression , B cell percentage and lower CD 4 : CD 8 ratios and increased HLA DR expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In type 1 GR lungs , anti IL 4 and anti IL 13 augmented IFNgamma and TNFalpha mRNA . ^^^ In type 2 lungs , anti IL 13 did likewise , but anti IL 4 decreased TNFalpha without affecting IFNgamma mRNA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Moreover , this compound showed substantial suppression of the Type 1 cytokines , IFNgamma ( 42 % ) , IL 2 ( 54 % ) , and TNFalpha ( 55 % ) . ^^^ Similarly , compound 16b showed a substantial enhancement of IL 4 ( 46 % ) and suppression of IL 2 ( 35 % ) , IFNgamma ( 30 % ) , and TNFalpha ( 26 % ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although ICAM 1 is expressed constitutively at low levels on endothelial cells and on some lymphocytes and monocytes , its expression can be significantly increased in the presence of cytokines ( TNFalpha , IL 1 , IFNgamma ) and reactive oxygen species . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the levels of TNFalpha , IFNgamma , and IL 10 mRNA expression in intestinal tissues resected from the patients with Crohn disease ( CD ) ( n=29 ) , ulcerative colitis ( UC ) ( n=8 ) , and controls ( n=8 ) using reverse transcription polymerase chain reaction ( RT PCR ) . ^^^ Compared with controls , tissues in CD showed high levels of TNFalpha and IFNgamma mRNA expression both in inflamed and non inflamed tissues , and showed high levels of IL 10 mRNA expression in inflamed tissues . ^^^ In UC , high levels of IL 10 mRNA expression were detected both in inflamed and non inflamed UC tissues , while those of TNFalpha and IFNgamma were not . ^^^ These findings suggest the overexpressions of TNFalpha and IFNgamma in CD , and less producibility of IL 10 against these cytokine might lead to development of severe CD . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study , we investigated whether the expression of messenger RNA ( mRNA ) encoding the interleukin ( IL ) 12 subunits p 40 and p 35 , IL 12 receptor chains , IL 18 , tumor necrosis factor alpha ( TNFalpha ) , IFNgamma , IL 10 , IL 4 , or transforming growth factor beta in unstimulated whole blood of 26 RR MS patients changed during 6 months of IFNbeta 1b treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Expression of IFNgamma , coexpression of TNFalpha and matrix metalloproteinases and apoptosis of T lymphocytes and macrophages in granuloma annulare . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Eosinophils and mRNA positive cells ( in situ hybridization for IL 2 , IL 3 , IL 4 , IL 5 , IL 6 , IL 8 , IL 10 , IL 13 , IFNgamma , RANTES and TNFalpha ) were counted in the biopsies . ^^^ After allergen provocation , levels of most of the measured cytokines ( IL 3 , IL 5 , IL 6 , IL 13 , IFNgamma , RANTES and TNFalpha ) and eosinophils were reduced using corticosteroids . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , suppression of TNF and other cytokines such as GM CSF , IL 6 , IFNgamma , and IL 12 has also been seen following exposure to high affinity and psychoactive ligands such as marijuana and THC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vitro IL 12 production by Th 1 type granuloma macrophages was enhanced by tumour necrosis factor ( TNF ) alpha and IFNgamma , whereas lymphocyte IL 12 responsiveness was boosted only by TNF alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , the spontaneous as well as TNFalpha induced secretion of ESM 1 is strongly inhibited by IFNgamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Addition of TNFalpha ( 10 ng / ml ) and IFNgamma ( 20 ng / ml ) enhanced the cytotoxicity of oxysterols and potentiated apoptosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNFalpha secretion was dose dependently stimulated by IVIg in both untreated microglia and after stimulation with LPS or IFNgamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Before DBPCFC , combined skin prick and patch testing was performed for cow milk , egg , soybean and cereals , and production of IFNgamma , IL 4 , IL 10 and tumour necrosis factor alpha ( TNFalpha ) was determined in supernatants of cultures of peripheral blood mononuclear cells ( PBMCs ) stimulated by cow milk . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Explants were incubated with pro ( IFNgamma , IL 1beta , and TNF alpha ) and anti ( IL 10 ) inflammatory cytokines and dexamethasone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Therefore , production of IFNgamma , TNFalpha , IL 2 , IL 6 and IL 10 was followed during the course of infection by cytokine assay in the blood and mRNA detection in the spleen . ^^^ IL 6 and IFNgamma were the two major cytokines detected whereas TNFalpha production was never observed . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Systemic administration of the bacterial endotoxin lipopolysaccharide ( LPS ) markedly increases the expression and activity of iNOS in both white and brown adipose tissues , as well as in skeletal muscle . iNOS induction can be reproduced in vitro by treatment of cultured white or brown adipocytes or L 6 myocytes with LPS and inflammatory cytokines ( TNFalpha , IFNgamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In plasma samples from 32 patients , we measured TNFalpha , IL1beta and IL 6 ( before transplant and on postoperative days 1 , 2 and 3 ) and IL 2 , IFNgamma , sIL2R and slCAM ( postoperative days 5 , 7 and 10 ) and examined possible correlations with T cell recovery and occurrence of rejection within 3 weeks . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To determine a possible mechanism for the regulation of surface expression of CD44st , v 5 and v 6 , we incubated Panc 1 cells with bFGF , TGF beta 1 , EGF , TNFalpha , and IFNgamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Rather , IFNgamma , produced by alloactivated T cells , seems to be a necessary ' first ' signal for nitric oxide production , while TNFalpha and CD40L each provide independent ' second ' signals . ^^^ These results suggest that macrophage nitric oxide production during a T dependent immune response requires IFNgamma production by CD 4 cells whereas TNFalpha and CD40L can each provide important functionally overlapping ' second ' signals to costimulate nitric oxide production , though neither is required . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The presence of mRNA encoding pro inflammatory cytokines ( TNFalpha , IL 1beta and IFNgamma ) in infected organs was measured using RT PCR , all three being present from 2 h post infection onwards , but not before . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
ELISA and RT PCR were used to examine IL 2 , IL 4 , IL 10 , TNFalpha , IFNgamma , IL I 0 concentrations and mRNA expression in the supernatant and cells from parallel cultures harvested at specific intervals . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Effect of IFNgamma , TNFalpha and IL 1beta on viability , butyrate oxidation and IL 8 secretion . ^^^ We investigated the effects of TNFalpha , IFNgamma and IL 1beta on viability , short chain fatty acid ( butyrate ) oxidation and IL 8 secretion in human colonic epithelial cell cultures in vitro obtained from macroscopically normal mucosa from IBD patients and controls . ^^^ Colonic crypts were isolated from endoscopical biopsies by ultra short ( 10 min ) EDTA / EGTA treatment , and exposed to TNFalpha , IFNgamma and IL 1beta for 24 hours . ^^^ This effect was more pronounced than that observed after addition of TNFalpha ( median 88 % ) ( P < 0 . 05 ) , but not IFNgamma alone ( median 78 % ) , whereas IL 1beta had no significant effect . ^^^ Cells from IBD patients were significantly less sensitive to TNFalpha + IFNgamma exposure ( median 74 % ) compared to cells from controls ( median 58 % ) ( P < 0 . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
All rats displayed increasing in situ IFNgamma levels as infection progressed , but no constant relationship was evident between IFNgamma and initial IL 1alpha / TNFalpha levels in O 3 exposed hosts . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Simultaneous addition of IFNgamma plus TNFalpha and IL 12 during hsp 10 stimulation could partially upregulate the low cytotoxic response observed in MB ( + ) by enhancing class 2 restricted T cell activity and by development of gammadelta T and / or CD16+56+ cell activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although we could induce caspase 8 in HFA with the inflammatory cytokines IFNgamma and TNFalpha , HFA remained resistant to Fas mediated injury . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The increased resistance that develops after primary infection / vaccination requires T cells cytokines such as IFNgamma TNFalpha and IL 12 in addition to opsonising antibody . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : The presence of mRNA coding for IL 6 , IL 8 , IL 1beta , IL 1alpha , TNFalpha , IFNgamma , IL 12 , and HPRT was investigated in 25 vitreous samples from patients with PVR , 11 vitreous samples from patients with retinal detachment ( RD ) not complicated by PVR , and 10 vitreous samples from patients with macular hole ( MH ) . ^^^ RESULTS : A strong correlation was found between mRNA and their respective cytokine levels ( protein products ) for IL 6 , IL 8 , IL 1beta , IL 1alpha , TNFalpha , IFNgamma ( Spearman r = 0 . 83 , 0 . 73 , 0 . 67 , 0 . 91 , 0 . 73 , and 0 . 73 respectively ) , but not for IL 12 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cells were also stained for the activation marker CD 69 or for the production of cytokines such as interferon gamma ( IFNgamma ) or tumor necrosis factor alpha ( TNFalpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Changes of immunological ( TNFalpha , IL 1beta , IL 2 , IL 2R , IL 6 , IFNgamma , TXB 2 , 6kPGF1alpha , sICAM 1 , sELAM 1 , sHLA 1 Ag ) and pathophysiological ( GOT , GPT , LDH , CK ) parameters were followed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Soluble IL 1beta was also increased , as measured by ELISA , and there was an increase in mRNA species for IL 1beta , IL 6 , TNFalpha but not IFNgamma or iNOS in these brains . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After stimulation with interferon gamma ( IFNgamma , 5U / ml ) or TNFalpha ( 1 ng / ml ) , there was a small increase in CD 40 mRNA and protein levels ; when both cytokines were applied , the increase in CD 40 levels was more than threefold . ^^^ Pretreatment of HPMC with TNFalpha and IFNgamma increased the response to CD 40 ligation in magnitudes that correlated with the elevation of CD 40 levels induced by the pretreatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 18 is a potent proinflammatory cytokine able to induce IFNgamma , GM CSF , TNFalpha and IL 1 in immunocompetent cells , to activate killing by lymphocytes , and to up regulate the expression of certain chemokine receptors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Dispersed bovine luteal cell cultures were challenged with increasing levels of bovine recombinant IFNgamma ( 0 1000 U ) or IFNgamma ( 200 U ) in the presence or absence of tumor necrosis factor alpha ( TNFalpha , 10 ng / ml ) over time ( short term , 0 60 min ; long term , 0 , 24 , 48 h ) . ^^^ Utilizing antibodies that recognize the nonphosphorylated forms of STAT 1 and STAT 3 , it was determined that levels of STAT 1 and STAT 3 in total cell lysates were constitutively expressed and did not change in response to treatment with IFNgamma or TNFalpha . ^^^ Furthermore , IFNgamma and TNFalpha treatment elevated levels of IRF 1 within 2 h . ^^^ TNFalpha induced increases in the levels of IRF 1 were transient , whereas the levels of IRF 1 in response to IFNgamma treatment remained elevated at 48 h . ^^^ IFNgamma treatment did not alter the levels of IkappaB alpha and failed to inhibit the TNFalpha initiated decrease in the levels of IkappaB alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We hypothesized that IFNalpha , which shares many biological activities with IFNgamma , might also synergize with TNFalpha for the induction of tumor cell death . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine production ( TNFalpha , IL 2 , IFNgamma , IL1beta , IL 10 ) in whole blood cell culture ( WBCC ) was determined at M 0 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNFalpha and IFNgamma induced by innate anti adenoviral immune responses inhibit adenovirus mediated transgene expression . ^^^ However , the cytokines interferon gamma ( IFNgamma ) and tumor necrosis factor alpha ( TNFalpha ) inhibit transgene expression from adenoviral vectors in vitro by a transcription related mechanism , and their early induction following vector administration in vivo suggests a contribution of innate immunity in regulating transgene expression . ^^^ In this study , the significance of cytokine expression and its relation to adaptive and innate immunities were determined in TNFalpha knockout mice , IFNgamma knockout mice , or anti IFNgamma mAb injected animals . ^^^ We conclude that TNFalpha and IFNgamma inhibit early adenovirus mediated transgene expression by HCMV and MCMV promoters in vivo . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine gene expression of Th 1 related cytokines ( IL 12 , IFNgamma and TNFalpha ) in adult PBMC was more evident to live than to heat killed promastigotes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , we showed that this profile was combined with a weak production of Th 1 related cytokines ( TNFalpha and IFNgamma ) and was associated with an absence of proliferation to the antigen . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A quantitative competitive RT PCR method was developed in order to measure IL 1beta , IL 4 , IL 12 , IFNgamma , TNFalpha and G ( 3 ) PDH mRNA from samples of ovine tissue such as lymph node or spleen . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Non fractionated whole blood and peripheral blood mononuclear cells ( PBMCs ) were obtained from male monkeys of different ages ( 6 28 years ) , and were subsequently evaluated for their ability to express mRNA and protein for the cytokines , IL 10 , IL 6 , IFNgamma , IL 1beta , and TNFalpha , following in vitro stimulation with polyclonal mitogens . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This technique allows determination of six human cytokine ( IFNgamma , TNFalpha , IL 2 , IL 4 , IL 5 , IL 10 ) concentrations simultaneously in a single tear sample . ^^^ Tears from allergic donors contained significantly less IL 10 ( p=0 . 035 ) , and had significant increases in the ratios of TNFalpha / IFNgamma , IL 5 / IFNgamma and IL 5 / IL 10 ( p=0 . 0008 , 0 . 0124 and 0 . 011 , respectively ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
T 84 cells produced NO in response to LGG when first primed with a combination of IL 1beta , TNFalpha and IFNgamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Further , IFNgamma may have a synergistic interaction with intrinsic TNFalpha in the brain . . ^^^ Although a tumor necrosis factor receptor fragment did not affect any sleep parameters when given at dark onset , it significantly attenuated IFNgamma induced NREMS and Tbr . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although IL 6 and tumor necrosis factor alpha transcripts were clearly evident in the synovial cells from the arthritic paws of IFNgamma / mice , neither message was elevated to the levels measured for IL 1beta expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Following re stimulation in vitro with PMA and ionomycin , CD4+ T cells produced IFNgamma , TNFalpha , TNFbeta , IL 2 , IL 4 , IL 10 and IL 13 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Infusions were safe and induced measurable serum levels of IFNgamma , TNFalpha , and IL 4 in two patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Preferential production of IL 4 , IL 10 and TGFbeta occurs on donor specific restimulation in vitro , with decreased production of IL 2 , IFNgamma and TNFalpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Classically , the induction of apoptosis in in vitro preparations has utilized the cytokines TNFalpha and IFNgamma and has been measured using the TUNEL technique . ^^^ Cytotrophoblasts from term placentae ( n=12 ) , syncytiotrophoblasts ( n=12 ) and SGHPL 4s ( n=8 ) were cultured under reduced oxygen or with TNFalpha / IFNgamma , dexamethasone or staurosporine . ^^^ With stimulation , these levels were significantly elevated using dexamethasone , TNFalpha / IFNgamma and staurosporine and further raised under hypoxic conditions . ^^^ SGHPL 4 cells showed similar trends to those of cytotrophoblasts , however the syncytiotrophoblasts , although responsive to dexamethasone and TNFalpha / IFNgamma , showed lower levels of apoptosis with staurosporine and hypoxia . ^^^ Although elevated with dexamethasone , staurosporine and hypoxia , these levels were markedly raised in cytotrophoblasts and SGHPL 4 cells following incubations with TNFalpha / IFNgamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Type 1 ( IL 2 , IL 12 , IFNgamma ) and pro inflammatory ( TNFalpha , IL 1beta , IL 6 ) cytokines were augmented in CD4+ , CD8+ , and CD14+ cells of acute MS patients and of patients undergoing disease reactivation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The proportion of PBMCs capable of producing IFNgamma and TNFalpha did not change , while Th 1 cytokine production by stimulated LPMC was decreased after infliximab therapy . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Previously , we demonstrated that the combination of the proinflammatory cytokines TNFalpha with IFNgamma induces the production of Abeta 42 and Abeta 40 in human neuronal cells . ^^^ In the present study , the neuronal cell line Sk n sh was incubated for 12 h with the cyclooxygenase inhibitor ibuprofen and subsequently stimulated with the cytokines TNFalpha and IFNgamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
FZD 10 mRNA was undetectable in MKN 45 cells , and was not up regulated by IFNgamma and TNFalpha in MKN 45 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The aim of this study was to estimate the influence of corticosteroids on Th 1 and Th 2 serum cytokine balance in patients with GO : IFNgamma , TNFalpha , IL 4 and IL 10 . ^^^ The levels of IFNgamma , TNFalpha , IL 4 and IL 10 in the serum were determined using ELISA . ^^^ In corticosteroid responsive patients , the balance of serum cytokines IL 4 / IFNgamma , IL 4 / TNFalpha , IL 10 / IFNgamma and IL 10 / TNFalpha increased and remained upregulated until the end of the study . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
As a consequence of inflammatory stimuli ( such as TNFalpha and IFNgamma ) , some constitutive subunits of the proteasome , the principal mediator of nonlysosomal protein degradation , are replaced with other subunits , the large multifunctional proteases LMP 2 and LMP 7 , thus originating the immunoproteasome . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Depending on the circumstances , inflammatory cytokines such as IL 1 , TNF and IFNgamma have been shown to contribute to either the induction , progression or inhibition of this disease . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Chronic IFNbeta 1b treatment down regulates TNFalpha , IFNgamma and MCP 1 production except for TNFalpha in relapsing patients . ^^^ IFNbeta 1b , in vitro , increases MCP 1 , TNFalpha and IFNgamma spontaneous production in all patients . ^^^ Multivariate analysis suggests that MCP 1 production is dependent from clinical status and not from TNFalpha and IFNgamma production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Despite a large interindividual variation , we found similar differences in cytokine production before and after stimulation by 10 ng / ml and 10 microg / ml LPS between both groups : temporal arteries of GCA patients produced more interleukin ( IL ) 1beta ( p < . 05 ) and IFNgamma ( nonsignificant ) and less tumor necrosis factor ( TNF ) alpha ( p < . 05 ) and IL 6 ( nonsignificant ) than temporal arteries of controls . ^^^ CONCLUSIONS : The present pilot study , which requires further confirmation on a larger number of well defined patients with GCA , suggests that a specific arterial cytokine production profile might exist in GCA ( high IL 1beta + / IFNgamma and low TNFalpha ) , addresses the question of the mechanisms by which IL 1beta and TNFalpha might be differentially regulated at the level of the arterial cell wall , and supports the view that cultures of the temporal artery might be an interesting tool for evaluating the role of cytokines in GCA pathogenesis . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Combined treatment using IFNgamma with TRAIL , APO 1 , TNFalpha or cytotoxic drugs cooperated to trigger apoptosis in various resistant tumor cell lines derived from Ewing tumor , neuroblastoma or medulloblastoma , while single agents exerted only a minimal effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The purpose of this study was to determine the relationship of TNFalpha and IFNgamma release and endotoxin induced liver injury in splenectomized and nonsplenectomized rats . ^^^ TNFalpha , IFNgamma , and alanine aminotransferase ( ALT ) , a marker of hepatocellular damage , were measured in aortic blood . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFNgamma primed monocytes showed increased responsiveness to LPS in terms of phosphorylation of the interleukin 1 receptor associated kinase ( IRAK ; immediately downstream of the MyD 88 adapter protein ) , NF kB DNA binding activity , and , accordingly , of cytokine ( tumor necrosis factor alpha [ TNFalpha ] and interleukin 12 [ IL 12 ] ) production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neurotoxicity induced by LPS or Abeta 1 42 plus IFNgamma is blocked by inhibitors of NO synthesis and by the peroxynitrite ( ONOO ) decomposition catalysts FeTMPyP [ 5 , 10 , 15 , 20 tetrakis ( n methyl 4 ' pyridyl ) porphinato iron ( 3 ) chloride ] and FeTPPS [ 5 , 10 , 15 , 20 tetrakis ( 4 sulfonatophenyl ) prophyrinato iron ( 3 ) chloride ] but not by the TNF alpha inhibitor pentoxifylline . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we show that the deactivating effects of LPS , as well as the priming effects of IFNgamma and GM CSF or their capacity to restore tumor necrosis factor ( TNF ) production by LPS tolerized human monocytes are independent of the modulation of TLR 2 , TLR 4 , or MD 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Dendritic retraction induced by IFNgamma represents a specific cellular response because it occurs without affecting axonal outgrowth or cell survival , and it is not observed with tumor necrosis factor alpha or other inflammatory cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The 16 mer peptide induces the production of IL 6 , IL 10 , TNFalpha and IL1alpha but it does not have any effect on secretion of IFNgamma and IL 4 . ^^^ Whereas 20 mer peptide induces production of TNFalpha , IL1alpha along with induction of IFNgamma but it downregulates IL 4 , IL 6 and IL 10 production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFNgamma and TNFalpha account for a pro clonogenic activity secreted by activated murine peritoneal macrophages . ^^^ Thus , we challenged the various macrophage conditioned media with polyclonal antibodies against IFNgamma and TNFalpha , and found that the macrophage pro clonogenic activity was completely abolished in the presence of anti IFNgamma antibodies , but only partially inhibited by anti TNFalpha antibodies . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To explain these functional differences we have developed a multiprobe RNAase protection assay for 13 sheep cytokines ( IL 1beta , IL 2 , IL 3 , IL 4 , IL 6 , IL 8 , IL 10 , IL 12 , IL 18 , GMCSF , IFNgamma , TGFbeta and TNFalpha ) and two housekeeping genes ( ATPase and GADPH ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To examine if similar responses occur in horses , we investigated il 2 , il 4 , il 5 , il 10 , tnfalpha , ifngamma and tgfbeta messenger rna expression in large intestinal mucosa . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Local reconstitution of IFNgamma ( / ) mice at the site of inflammation with the IFNgamma gene increased the levels of TNFalpha and decreased the IL 10 levels . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This response relies on the concerted action of a number of cytokines ( TNFalpha , IFNgamma , IL 12 , IL 18 , and IL 15 ) , on the recruitment of inflammatory phagocytes in the tissues and on the activation of the recruited cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : We used enzyme linked immunosorbent assays to measure the concentration of selected cytokines ( interleukin 18 [ IL 18 ] , IL 15 , IL 12 , IL 2 , interferon gamma [ IFNgamma ] , and tumor necrosis factor alpha [ TNFalpha ] ) in sera from 58 patients with systemic autoimmune diseases and 33 healthy controls . ^^^ RESULTS : Levels of IL 18 , IL 15 , IFNgamma , and TNFalpha were elevated in sera from patients with systemic autoimmune diseases compared with normal controls . ^^^ The combination of IL 18 and IL 15 or IL 18 and IL 12 enhanced IFNgamma and TNFalpha production by NK cells in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
LPS activated Kupffer cells produced significant amounts of NO , TNFalpha and IFNgamma . ^^^ Therefore , Kupffer cells execute their anti tumor effect via increasing the production of NO , TNFalpha and IFNgamma and these cytotoxic molecules inhibit the growth of tumor by damaging cellular DNA and inducing apoptosis that was featured by downregulation of Bcl 2 but upregulation of caspase 8 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Two cytokines , TNFalpha and IFNgamma , were significantly elevated in the supernatant obtained from the activated macrophages . ^^^ Considering an important role of these two molecules in the induction of apoptosis mediated by the Fas / FasL system , the present data suggested that TNFalpha and IFNgamma were the main molecules to trigger the cascade of apoptotic reactions in glioma cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Unlike other proinflammatory cytokines ( TNFalpha and IFNgamma ) , IL 1beta was not toxic for oligodendrocyte lineage cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found that peripheral blood mononuclear cells ( PBMCs ) from MS patents spontaneously produce high levels of TNFalpha , TNFbeta , IFNgamma , and oncostatin M ( oncM ) , a proinflammatory cytokine actng on cells of neural , vascular , hematopoietic , and lymphoid origin . ^^^ Determination of TNFalpha , TNFbeta IFNgamma , and oncM in corresponding sera showed that on average , oncM levels were higher in MS patients than in HC , though the results were not statistically significant whereas levels of TNFalpha , TNFbeta and IFNgamma were below the assay threshold in most patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To induce TNF alpha production by PB T cells and monocytes , whole blood samples were stimulated either for 4 h with PMA plus ionomycin or for 6 h with LPS plus IFNgamma , respectively . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Human CEC isolates treated with IFNgamma , TNFalpha , and TA were evaluated by flow cytometry and immunocytochemistry for expression of intercellular adhesion molecule ( ICAM ) 1 , vascular cell adhesion molecule ( VCAM ) 1 , and major histocompatibility complex ( MHC ) 1 and 2 . ^^^ The effects of IFNgamma , TNFalpha , and TA on paracellular permeability of CEC monolayers were assessed in transendothelial cell resistance ( TER ) assays . ^^^ RESULTS : Both IFNgamma and TNFalpha significantly upregulated expression of ICAM 1 and MHC 1 on CECs . ^^^ Expression of VCAM 1 was induced after stimulation with both IFNgamma and TNFalpha , whereas expression of MHC 2 was induced only by stimulation with IFNgamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inflammation progresses by the action of pro inflammatory cytokines , including interleukin 1 ( IL 1 ) , the tumor necrosis factor ( TNF ) , gamma interferon ( IFNgamma ) , IL 12 , IL 18 , and the granulocyte macrophage colony stimulating factor , and is resolved by anti inflammatory cytokines such as IL 4 , IL 10 , IL 13 , IFNalpha , and the transforming growth factor ( TGF ) beta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In vivo blocking experiments with antagonistic monoclonal antibodies indicated that the early lethality was mediated by FasL , IFNgamma and TNFalpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNF , IFNgamma , IL 2 , IL 4 , IL 6 , IL 10 and IL 12 were measured in plasma and liver tissue after either Concanavalin A ( Con A ) , D galactosamine / lipopolysaccharide ( GalN / LPS ) or high dose LPS induced liver injury . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Analysis of graft infiltrating cells , IL 10 , TNFalpha , IFNgamma mRNA and iNOS protein expression in allografts , 5 days after transplantation , showed that they were markedly decreased in allografts from LF 15 0195 treated recipients compared with allografts from untreated recipients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increased susceptibility was accompanied by a reduced Th 1 response , demonstrated by decreased levels of IL 12 , IFNgamma , and tumor necrosis factor alpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Analysis of cytokine production demonstrated that E 2 decreased TNFalpha , IFNgamma and IL 12 production in mature DCs . ^^^ In addition , MBP specific T cells cocultured with E 2 pretreated mature DCs in the presence of antigen demonstrated a shift towards production of Th 2 cytokines IL 4 and IL 10 and a concomitant decrease in the production of Th 1 cytokines TNFalpha and IFNgamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , the localizations of 4 cytokines ( IL 1alpha , IL 1beta , TNFalpha and IFNgamma ) in tendinocytes of the equine superficial digital flexor tendon ( SDFT ) were analyzed by the use of an immunohistochemical method . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Spleen cells from immunized mice , after exposure to recombinant MPT SOD ( MPT rSOD ) , produced significant levels of IFNgamma , TNFalpha and IL 6 . ^^^ IFNgamma and TNFalpha production was increased by the addition of Ribi . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
AIMS : To examine the effects of 5 HT moduline on the stimulated production of IFNgamma , tumor necrosis factor alpha ( TNFalpha ) and IL 10 . ^^^ RESULTS : 5 HT moduline , 10 ( 6 ) M and 10 ( 5 ) M , significantly reduced the production of IFNgamma and the IFNgamma / IL 10 ratio . 5 HT moduline 10 ( 5 ) M significantly reduced the production of TNFalpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment of normal and SSc fibroblasts with tumor necrosis factor a ( TNFalpha ) , IL 2 , IL 4 , IL 6 , IL 10 , interferon alpha ( IFNalpha ) , IFNgamma , granulocyte macrophage colony stimulating factor ( GM CSP ) , G CSF , or connective tissue growth factor ( CTGF ) had no effect on ROS generation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We also investigated whether certain cytokines , such as TNFalpha and IFNgamma might induce PAF synthesis in our systems of cell lines which we found to express mRNAs encoding receptors for these cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines that were consistently detectable were IL 6 ( mean 726 . 20 pg / ml ) , TNFalpha ( 50 . 64 pg / ml ) , IFNgamma ( 39 . 64 pg / ml ) , GM CSF ( 216 . 12 pg / ml ) , and MCP 1 ( 154 . 43 pg / ml ) ; IL 1beta , IL 5 , and IL 13 were undetectable . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
During the primary infection , ACRS caused a significant delay in clearance of LM , loss of body weight , reduced food / water intake , and elevated levels of pro inflammatory cytokines ( IL 6 , IL 1beta , and TNFalpha ) and IFNgamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
While infliximab induced a downregulation of the production of the T helper 1 cytokines IFNgamma and TNFalpha , etanercept treatment triggered rather an upregulation of these cytokines secreted by T cells after in vitro stimulation . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
While amounts of TNFalpha and IL 6 remained nearly unchanged , IFNgamma enhanced the production of IL 12 , MCP 1 and RANTES , but attenuated that of KC , MIP 1alpha and MIP 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using flow cytometry , intracellular cytokine expression of IFNgamma and TNFalpha was determined in the T cell population . ^^^ The monocytes were evaluated for IL 6 , IFNgamma , IL 12 , and TNFalpha . ^^^ For both patient groups , the number of IFNgamma expressing T cells fell significantly at re infusion , whilst both T cell and monocyte expressing TNFalpha levels were reduced at re infusion . ^^^ However , the reduction in the number of IFNgamma and TNFalpha expressing mononuclear cells means , at this early stage , it is unlikely that these cytokines assist in the removal of the malignant Th 2 cells present in CTCL . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It was determined the following data : production of IL 2 ( in 5 patients and 5 controls ) , its receptor ( in all participants ) , IFN g ( in 8 patients and 5 controls ) , and TNF a ( in 8 patients and 5 controls ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
AIM : The expression of the cytokines IL 2 , IL 6 , IL 10 , IFN g and TNF a and the adhesion proteins CD 99 and CD 106 was studied in the human testis at the protein level . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The release of interleukin ( IL ) 1alpha , IL 2 , IL 6 , IL 8 , IL 10 , IL 12p ( 40+70 ) , IL 12p ( 70 ) , tumor necrosis factor alpha ( TNFalpha ) , interferon ( IFN ) alpha , IFNgamma , granulocyte macrophage colony stimulating factor , and RANTES was determined after incubation with 0 . 5 ng / mL of IQuS ng / mL and VaQuFrF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using purified primary human fetal astrocyte cultures , we determined chemokine production using ELISA assays and RT PCR and chemokine receptor expression using immunocytochemistry and RT PCR with QUIN in comparison to TNFalpha , IL 1beta , and IFNgamma . ^^^ Most of these effects were comparable to those induced by TNFalpha , IL 1beta , and IFNgamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Transmembrane TNF and IFNgamma induce caspase independent death of primary mouse pancreatic beta cells . ^^^ We have shown that TNF + IFNgamma induce islet cell death in vitro . ^^^ TNF + IFNgamma did not induce caspase 3 activation in primary mouse islets . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Peritoneal macrophages pre exposed to the molecule are less able to produce the cytokines IL 12 , IL 6 and TNF alpha following subsequent incubation with the classical stimulators IFNgamma and LPS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cultured glial cells from the cerebellum of 15 day old taiep rats produced NO , increased iNOS levels , up regulated iNOS expression and promoted TNF release when stimulated with LPS and IFNgamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Exposure of hMSCs to IL 1alpha , TNFalpha or IFNgamma up modulated ICAM 1 surface expression , whereas only IFNgamma increased both HLA class 1 and class 2 molecules on the cell surface . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Furthermore , addition of caspase inhibitors or anti TNFalpha / IFNgamma monoclonal antibodies to fludarabine treated cells allowed us to determine the mediators of apoptosis . ^^^ Caspases 1 and 6 were the major caspases involved in fludarabine apoptosis induction in resting B cells , while only anti TNFalpha / IFNgamma monoclonal antibodies reduced apoptosis in activated cells . ^^^ In agreement with this observation , autocrine production of TNFalpha and IFNgamma by CD 40 activated CLL B cells was found . ^^^ Moreover , upregulation of TNFRI / II , release of TNFalpha and IFNgamma , and inhibition of apoptosis by anti TNFalpha / IFNgamma monoclonal antibodies in CD 40 activated cells strongly suggest that these cytokines may play a role in sensitizing B cells to fludarabine treatment . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Whereas IL 18 and TNFalpha induced the protease , MMP 9 release was not mediated by IFNgamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In stimulating with LPS + IFNgamma , DCs of HCC patients expressed significantly high levels of CD 86 ( p < 0 . 05 ) and produced high levels of IL 12 as compared to DCs stimulated with TNFalpha alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PCR based genotyping was performed for the genes encoding TNFalpha ( 308G / A ) , TGFbeta 1 ( codon 10C / T , codon 25C / G ) , IL 10 ( 1082A / G , 819T / C , 592A / C ) , IL 6 ( 174C / G ) and IFNgamma ( 874T / A ) . ^^^ RESULTS : SNP allelic and genotypic frequencies for IL 6 and IFNgamma , but not for TNFalpha , TGFbeta 1 , and IL 10 , differed significantly between the Caucasian and African American women . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pro inflammatory cytokine ( IL 6 , IL 1beta , and TNFalpha ) and IFNgamma levels positively correlated with the LM levels in all groups of mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
For simplicity of discussion , three categories of cytokines are discussed : ( 1 ) . the proinflammatory cytokines TNFalpha , IFNgamma , IL 1 , and IL 6 ; ( 2 ) . the chemokines MIP 1 , CINC 1 and IL 8 ; and ( 3 ) . other cytokines ( LIF , CNTF , GM CSF , FGF , EGF , and TGFalpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A significant increase in the production of Th 1 type cytokines [ IFNgamma , interleukin 2 ( IL 2 ) , and tumor necrosis factor alpha ( TNFalpha ) ] was found at the end of the treatment in complete responders compared with baseline values ( P < 0 . 001 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Following stimulation in culture , PEC from DOX treated mice produced more TNF , IL 1 , and IFNgamma than PEC from untreated mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Differential and cooperative effects of TNFalpha , IL 1beta , and IFNgamma on human conjunctival epithelial cell receptor expression and chemokine release . ^^^ PURPOSE : To gain better understanding of conjunctival epithelial cell responses to proinflammatory cytokines , the individual and combined effects of TNFalpha , IL 1beta , and IFNgamma on chemokine release ( IL 8 , regulated on activation normal T cell expressed and secreted [ RANTES ] ) and surface receptor expression ( intercellular adhesion molecule [ ICAM ] 1 , and HLA DR , DP , and DQ ) were examined . ^^^ RESULTS : TNFalpha , IL 1beta , and IFNgamma had distinctive individual and combined effects on the parameters tested . ^^^ Release of RANTES required two cytokine signals , with IFNgamma and TNFalpha being the most potent combination . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both proinflammatory ( IL 2 , IL 6 , IFNgamma , TNFalpha ) and anti inflammatory ( IL 4 , IL 10 ) cytokines were significantly increased in infected infants compared with non infected infants . ^^^ Significant correlations were observed between IL 6 and TNFalpha or IL 10 as well as IL 10 and IFNgamma in infected infants . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neutralization of IFNgamma or TNFalpha , present in liquid culture , also significantly increased CFU GM formation and CD34+ cell content . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the present study , we examined the effects of B [ a ] P , 2 BP , phenol and TCDD on proinflammatory cytokine gene expression in mice spleen cells which were stimulated with anti CD 3 . 10 ( 9 ) M TCDD increased IFNgamma and TNFalpha gene expression , but suppressed IL 1 gene expression . 10 ( 6 ) M phenol inhibited IL 1 , IL 6 and TNFalpha gene expression , and 10 ( 6 ) M of 2 BP downregulated TNFalpha gene expression . ^^^ However , 10 ( 6 ) M of B [ a ] P did not influence on IL 1 , IL 6 , IFNgamma and TNFalpha gene expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Immediately following ECP , a significant number of lymphocytes become apoptotic and the number of T cells producing TNFalpha and IFNgamma is reduced . ^^^ Both apoptotic and non apoptotic T cells were evaluated for their intracellular expression of IL 2 , IL 4 , IL 10 , IFNgamma and TNFalpha . ^^^ However the number of T cells expressing IL 2 , IFNgamma and TNFalpha was reduced in both the Annexin 5 positive and negative T cell populations ( P < . 05 ) . ^^^ The lowest number of IL 2 , IFNgamma and TNFalpha expressing T cells occurred within the apoptotic population ; however , Annexin 5 negative T cells also demonstrated a marked reduction post ECP . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There was no significant expression of IL 2 , IL 2 receptor , IL 4 , TNFalpha , or IFNgamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The early response was characterized by high levels of inflammatory molecules , including IL 1beta , IL 6 , TNFalpha , RANTES , MIP 1alpha and MCP 1 , later followed by expression of precursor Th 1 cytokines , IL 12 and IL 18 , concomitant with IFNgamma production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After incubation of the cells with IL 1beta ( 10 micro g / L ) , TNFalpha ( 100 micro g / L ) and interferon ( IFN ) gamma ( 100 000 U / L ) for 48 hours , the combination of TNFalpha and IFNgamma induced apoptosis in INS 1 cells but not in INS 1 / IkBDeltaN cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Also , changes in this gene profile were determined following stimulation with TNFalpha , IL 1beta , and IFNgamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
With the RT PCR method we evaluated mRNA levels for IL 1beta , IL 6 , TNF , IFNgamma , IL 10 and iNOS in the striatum of C57BL / 6 mice after MPTP intoxication . ^^^ The first increase of mRNA for TNFalpha and IFNgamma was noticed at 6 24 h and the second at the 7th day after MPTP intoxication . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the effects of glucose and beta cell growth factors ( IGF 1 , IGF 2 , bFGF ) on growth and apoptosis in the presence and absence of apoptosis inducing cytokines ( IFNgamma , Il 1beta , TNFalpha ) . ^^^ The cytokines IFNg and IL 1beta , but not TNFalpha strongly induced INS 1E beta cell apoptosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Constitutive active PKB / Akt protected HUVEC from death induced by zoledronate and TNF / IFNgamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The authors have demonstrated recently that acute B 19 infection is accompanied by raised circulating levels of IL 1b , IL 6 , TNF a , and IFN g and that raised circulating levels of TNF a and IFN g persist and are accompanied by MCP 1 in those patients who develop CFS . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Examination of the cytokines produced by these Th cells showed that a majority of the proliferative p 53 specific T cell cultures produced none of the key cytokines ( IFNgamma , TNFalpha , IL 4 , IL 5 or IL 10 ) , indicating that these p 53 specific Th responses are not polarized . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Exposure of cells to inflammatory mediators ( IFNgamma and / or TNFalpha ) resulted in increased expression of intercellular adhesion molecule ( ICAM ) 1 and HLA DR . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Suppression could not be ascribed to TGFbeta , IFNgamma , TNFalpha , CD 48 , or ICAM 1 , or to interactions between CD 40 and CD 154 , or CD 95 and CD 95 ligand . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
MLDSTZ treatment in vivo caused increased IFNgamma secretion , a decreased / retarded rate of increased TNFalpha accumulation , whereas IL 10 production was not altered compared to vehicle treated mice . ^^^ Production of TNFalpha , IFNgamma and IL 10 was all suppressed by the drug . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We identified 12 novel nucleotide substitution variants and one deletion variant in 17 genes analyzed ( TGFBETA ; R , BETA ; 2M , IFNGAMMA ; , TNFALPHA ; , TNFALPHA ; R , LTALPHA ; , IL 6 , IL 12 , IL 2 , IL 1ALPHA ; , IL 1BETA ; , IL 1RN , IL 10 , CTLA 4 , CD40L , FAS and FASL ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In experimental autoimmune encephalomyelitis , the acute phase of the disease is produced by T helper lymphocyte type 1 ( TH 1 ) , which produces mainly TNFalpha and IFNgamma . ^^^ To address the influence of TGFbeta on TH 1 induced gene expression , microarray technology was used on murine primary microglial cells stimulated with IFNgamma and TNFalpha in the absence or presence of TGFbeta . ^^^ TH 1 cytokines upregulated 175 genes comprising cytokine , chemokine , and genes involved in host response to infection and the TNFalpha / IFNgamma intracellular signaling pathway . ^^^ It is observed that TGFbeta inhibits expression of 25 % of the TNFalpha / IFNgamma induced genes and a further 66 TNFalpha / IFNgamma independent genes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : CH was evaluated in each group via oxazolone challenge following a 5 day exposure to intradermal ( ID ) cUCA or a single exposure to topical permethrin , or co exposure to both chemicals in 5 week old female C57BL / 6N , SvImJ , and C57BL / 6N mice genetically altered at the TNFalpha or IFNgamma locus . ^^^ CONCLUSIONS : These results suggest the following : ( 1 ) Mouse strains show variable susceptibility to permethrin and cUCA induced immunomodulation . ( 2 ) TNFalpha may be involved in the immunomodulatory effects of cUCA and permethrin . ( 3 ) IFNgamma may be required for the more than additive depression of CH caused by cUCA+permethrin . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we investigated PGE 2 production in primary rat astrocytes in response to agents that activate PLA 2 including pro inflammatory cytokines ( IL 1beta , TNFalpha and IFNgamma ) , the P 2 nucleotide receptor agonist ATP , and oxidants ( H2O2 and menadione ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cells expressing TNFalpha and IFNgamma but not IL1beta were present in stromal and superficial areas . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Baseline and sequential cytokine levels ( IL 1 , TNFalpha , IFNgamma , TGFbeta and IL 10 ) were examined after 52 weeks of infliximab treatment 5 mg / kg in 22 patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The mDC protein expression profile showed significant increases in 16 out of 34 factors tested , including TNFalpha , IL 10 , IL 12 , IFNgamma , MIP1alpha , MIP1beta , IL 8 , MDC , RANTES , and IL 6 , which play a crucial role in the regulation of the innate immune response as well as the recruitment and activation of adaptive immune effectors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Neither TNF 308 and 238 polymorphisms nor IFNgamma alleles variability were associated with different HCV outcome . ^^^ Given that polymorphisms in regulatory regions of cytokine genes influence cytokine production , we determined frequency of polymorphisms of IL 10 , IFNgamma , and TNFalpha genes in HCV infected patients and healthy controls , and investigated their association with either ongoing or cleared HCV infection , or with response to treatment . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Human B lymphoblast cell lines defective of Stat 6 signaling produce high levels of proinflammatory cytokines IL 12 , TNFalpha and IFNgamma . ^^^ Production of three important proinflammatory cytokines , IL 12 , TNFalpha and IFNgamma was examined in spontaneous EBV B cell culture using ELISA methodology . ^^^ Individual cell lines defined as Stat6null produced significantly higher levels of IL 12 , TNFalpha and IFNgamma on day 4 in spontaneous culture in comparison with cell lines characterized as Stat6high and Stat6low . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated and compared the effects of IFNgamma and TNFalpha in a primary culture of human conjunctiva and in a human conjunctival cell line . ^^^ METHODS : A primary cultured human conjunctival epithelium and a human conjunctival cell line ( Chang cells ) were treated for 72 hr with 20 , 200 , 400 and 600 U ml ( 1 ) IFNgamma or with 1100 and 11 , 000 U ml ( 1 ) TNFalpha . ^^^ TNFalpha and IFNgamma , two inflammatory cytokines , induced different effects in both cellular systems , in a primary cultured conjunctival epithelium and a human conjunctival cell line . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Eight week old apolipoprotein E ( apoE ) knockout mice were infected with mouse CMV ( MCMV ) or mock injected , and they were sacrificed at 2 and 20 weeks post injection ( p . i . ) to study atherosclerosis , vascular wall IFNgamma and TNFalpha expression and MCMV spread . ^^^ To study plasma IFNgamma and TNFalpha levels , blood was collected at 1 , 2 , 4 and 6 days p . i . in addition to days of sacrifice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment was accompanied by an early burst of cytokines ( IFNgamma , MCP 1 , TNFalpha , IL 2 , IL 4 , IL 5 , and IL 6 ) followed by sustained increases in IFNgamma and IL 4 transcripts in the spleen and aorta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In insulin treated Type 1 diabetic patients , naive and recently primed CD 4 ( + ) cells were characterised by increasd IFNgamma , TNFalpha , and IL 5 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The myeloid cell lines were incubated with phorbol esters , butyric acid and combination of TNFalpha and IFNgamma to induce differentiation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the role of Th1 / Th2 / Th3 cytokine gene polymorphisms , such as TGF beta 1 codon 10 ( TGFbetac 10 ; C to T ) , TGF beta 1 codon 25 ( TGFbetac 25 ; G to C ) , TNFalpha promoter 308 ( G to A ) , IL 6 promoter 174 ( G to C ) , IL 10 promoter 1082 ( G to A ) , IL 10 promoter 819 ( C to T ) , IL 10 promoter 592 ( C to A ) , and IFNgamma intron 1 +874 ( A to T ) in abortion prone female patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Peripheral blood lymphocytes of 18 patients with idiopathic intermediate uveitis and 6 patients with presumed sarcoid intermediate uveitis were evaluated for CD 4 ( + ) T cell expression of CD 69 , CCR 4 , CCR 5 , CXCR 3 and the intracellular cytokines IFNgamma , TNFalpha , and interleukin ( IL ) 10 by flow cytometry , and for IL 2 , IL 4 , IL 5 , IL 10 , IFNgamma , and TNFalpha production following unstimulated and activated culture using cytokine bead array and compared with healthy control subjects . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CEC , HT 29 or Caco 2 cells were incubated with either IFNgamma , tumor necrosis factor ( TNF ) alpha or IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We observed a weak expression of costimulatory molecules ( CD 80 , CD 86 ) and an absence of inflammatory cytokines ( IFNgamma , TNFalpha , IP 10 ) and lymphocyte activation markers ( CD 25 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These changes , known collectively as non thyroidal illness , are apparently mediated by the proinflammatory cytokines IL 6 , TNFalpha and IFNgamma . ^^^ IL 12 is involved in regulation of IFNgamma and TNFalpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Recruitment and proliferation of T lymphocytes is supported by IFNgamma and TNFalpha activated human osteoblasts : Involvement of CD 54 ( ICAM 1 ) and CD 106 ( VCAM 1 ) adhesion molecules and CXCR 3 chemokine receptor . ^^^ The effects of two regulatory cytokines , TNFalpha and IFNgamma , on the expression of CD 54 ( ICAM 1 ) and CD 106 ( VCAM 1 ) adhesion molecules and the CXCR 3 ligands ( CXCL 9 , CXCL 10 , CXCL 11 ) , were assessed in a primary culture of human OB by real time PCR , flow cytometry , and immunohistochemistry . ^^^ According to the present data IFNgamma , either alone or in combination with TNFalpha , significantly up regulates the expression of CD 54 and CD 106 and induces the expression and release of CXCL 9 , CXCL 10 , CXCL 11 in OB . ^^^ The supernatant of TNFalpha and IFNgamma activated OB induces the recruitment of T lymphocytes more significantly than stimulation by CXCR 3 ligands . ^^^ T lymphocyte proliferation is significantly enhanced by direct contact with TNFalpha and IFNgamma activated OB or by incubation with the supernatant of TNFalpha and IFNgamma activated OB . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Levels of TNFalpha , IL 6 , ACTH , and cortisol , titres of anti hemagglutinins and anti neuraminidases , lymphocytes secreting IFNgamma , IL 2 , IL 4 , and IL 10 , cytotoxicity of NK and CD3+ CD8+ IFNgamma+ cells , anti CMV antibodies , and CD 28 CD57+ lymphocytes known to be associated with the CMV carrier status were evaluated . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These studies suggested that priming the lung with IFNgamma could enhance proinflammatory ( IL1beta , IL 6 , TNFalpha ) cytokine gene expression and suppress interferon gene expression compared to IT poly [ rI . rC ] alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Up to 200 unrelated German RA and JRA patients each and 300 400 healthy controls have been genotyped for HLA DRB 1 , TNFa , TNFA 238a / g , TNFA 308a / g , TNFA 857c / t , TNFR 1 609g / t , TNFR 1 P12P , TNFR 2 del 15bp , IKBL 332a / g , IKBL 132t / a , IKBL C224R , CTLA 4 318c / t , CTLA 4 T17A , PTPRC P57P , MIF 173g / c , the MIF and IFNG microsatellites as well as for D17S795 , D17S807 , D17S1821 by polyacrylamide gel electrophoresis , single strand conformation polymorphism analysis , restriction fragment length polymorphism analysis or allele specific hybridization . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
No SNPs at IFNG , IL 2 , IL12B , TNF , or CCL 2 ( MCP 1 ) showed any association with HIV related outcomes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Fas expression is thought to be induced by proinflammatory cytokines , such as IL 1beta , interferon gamma ( IFNgamma ) , and TNFalpha , released by islet infiltrating mononuclear cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Gametocytes are capable of inducing specific humoral IgG , and cellular responses , which include induction of TNFalpha , IFNgamma and gammadelta+ lymphocyte proliferation , in addition to immune responses to other stages of the parasite ( sporozoite , exo erythrocytic stages , erythrocytic stages ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This effect is not reproduced with IFNgamma or TNFalpha alone , implicating the interaction of multiple factors in the overall functional response . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Draining lymph node cells of transgenic mice secreted more tumor necrosis factor alpha and IFNgamma and proliferated more vigorously in response to collagen type 2 and upon CD3 / CD28 costimulation in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
After 6 h incubation with either IL1beta , TNFalpha , IFNgamma , or combinations of TNFalpha and IFNgamma , ENaC was measured as electrogenic Na+ transport after 8 h induction by 3 nM aldosterone ( JNa ) in totally stripped specimens in the Ussing chamber . ^^^ In parallel , beta and gamma ENaC transcription was inhibited , whereas alpha ENaC was unaffected . gamma ENaC promoter activity was inhibited by IL 1beta and TNFalpha but not by IFNgamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Levels of the cytokines interferon gamma ( IFNgamma ) , IL 10 , and tumor necrosis factor alpha ( TNFalpha ) produced by cultured peripheral blood mononuclear cells ( PBMC ) were measured in culture supernatants following T cell stimulation in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The aims of the present study were to examine 1 ) the effects of CRH , 10 9 M , 10 ( 8 ) M and 10 ( 7 ) M , on the stimulated production of IFNgamma , IL 10 and tumor necrosis factor alpha ( TNFalpha ) by human whole blood ; and 2 ) whether CRH , 10 ( 9 ) M , 10 ( 8 ) M and 10 ( 7 ) M , may antagonize some of the negative immunoregulatory effects of 5 HT , 1 . 5 microg / mL or 15 microg / mL . ^^^ We found that CRH , 10 ( 9 ) M , 10 ( 8 ) M and 10 ( 7 ) M , had no significant effects either on the stimulated production of IFNgamma , IL 10 or TNFalpha or on the IFNg / IL 10 production ratio , which reflects the pro inflammatory capacity of the culture . 5 HT , 1 , 5 microg / dL and 15 microg / dL , significantly suppressed the production of IFNgamma and TNFalpha and the IFNg / IL 10 production ratio . ^^^ The results suggest that CRH has no significant direct effects on the production of IFNgamma , IL 10 and TNFalpha , but antagonizes the negative immunoregulatory effects of 5 HT on the production of IFNgamma and TNFalpha and on the IFNgamma / IL 10 production ratio . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here , we investigated the sensitivity of the Ewing tumor cell line , SK N MC , to the apoptotic effect of type 1 ( IFNalpha ) and type 2 ( IFNgamma ) interferons and TNFalpha . ^^^ We demonstrate that although IFNalpha and TNFalpha alone are unable to induce cell death , they act in synergy with IFNgamma to induce SK N MC cell apoptosis . ^^^ We have shown that the presence of IFNgamma is required to allow the sustained expression of IRF 1 in SK N MC cells stimulated by addition of IFNalpha or TNFalpha suggesting that IRF 1 plays a role in the synergistic induction of apoptosis by combinations of cytokines . ^^^ Furthermore , we have shown that inhibition of NF kappaB activation contributes to the IFNgamma mediated sensitization to the apoptotic effect of TNFalpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 1beta and TNF , alone or in combination with IFNgamma , failed to induce IkappaBalpha or IkappaBbeta degradation in HT 29 cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Bronchoalveolar lavage fluid ( BALF ) from NOS 2 deficient mice contained significantly elevated TNF activity , IFNgamma and total protein during mid / late infection . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : The production of proinflammatory ( TNFalpha , IL 1 , IL 6 ) , Th 1 ( IL 12 , IFNgamma ) , and Th 2 ( IL 10 ) cytokine profiles of immature DC ( iDC ) from patients with RA and healthy subjects upon triggering of FcgammaR dependent and independent pathways was investigated . iDC , derived from blood monocytes by standardised protocols , were stimulated with immune complexes ( IC ) at day 6 for 48 hours and , subsequently , for 2 days with LPS in the presence or absence of IC or IFNgamma , resulting in fully matured DC ( mDC ) . ^^^ IL 1 , IL 6 , TNFalpha , IFNgamma , IL 12 , and IL 10 levels in supernatants were measured by ELISA and RIA . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study examines the effects of pro inflammatory cytokines , interleukin 1beta ( IL 1beta ) , gamma interferon ( IFNgamma ) and tumor necrosis factor alpha ( TNFalpha ) on human beta defensin 2 ( HBD 2 ) levels in cultured keratinocytes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using this protocol , we were able to identify human proteosome subunit Y as a reliable endogenous reference gene for human umbilical vein endothelial cells treated for up to 18 h with TNFalpha , IL 4 , or IFNgamma and for B cells isolated from healthy controls and patients suffering from IgA nephropathy . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Previously , it was shown that proinflammatory cytokines characteristic of the APR ( TNF alpha , IL 1beta , and IFNgamma ) induced the expression of the PRL receptor ( PRLR ) by pulmonary fibroblasts in vitro . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Brain endothelial cells were incubated with either sodium nitroprusside ( SNP , 10 microM ) , inflammatory proteins ( IL 1beta , IL 6 , TNFalpha , LPS , IFNgamma ) or the PKC inhibitor bisindolymaleimide ( 1 microM ) for 24 h and conditioned media collected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The IFNgamma produced by PMN was biologically active , as demonstrated by its ability to induce TNFalpha synthesis by PMN themselves or to induce IL 10 synthesis by peripheral blood mononuclear cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RDP 58 is a 10 amino acid peptide with two major activities : ( 1 ) inhibition of inflammatory TH 1 cytokines such as TNFalpha , IFNgamma , and IL 12 and ( 2 ) up regulation of heme oxygenase 1 ( HO 1 ) expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pseudomonas aeruginosa induced production of free radicals by IFNgamma plus TNFalpha activated human endothelial cells : mechanism of host defense or of bacterial pathogenesis . ^^^ We have previously shown that human umbilical vein endothelial cells ( HUVEC ) can be activated by IFNgamma plus TNFalpha to kill intracellular ( IC ) Pseudomonas aeruginosa through production of reactive oxygen intermediate , but the cumulative effects of cytokine activation and bacterial infection on host cells has not been extensively addressed . ^^^ In this study we investigated the fate of IFNgamma plus TNFalpha activated HUVEC that have harboured IC bacteria for up to 24 h . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The pro inflammatory cytokine , IFNgamma , also abolishes suppression of TNFalpha in LPS tolerance . ^^^ In addition , IFNgamma pretreatment reversed LPS tolerance as evidenced by increased TNFalpha levels while IL 6 , IL 1beta , and GMCSF levels were further augmented . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Coapplication of CGA with betaA or with IFNgamma resulted in a synergistic effect on NO and TNFalpha secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We investigated the influence of the degree of dilution of whole blood and the duration of the incubation period on whole blood as well as peripheral blood mononuclear cells ( PBMCs ) , cultured in the absence or presence of mitogens , superantigens or specific antigens , for intracellular cytokine production ( IFNgamma , TNFalpha , IL 2 , IL 4 , IL 10 and IL 13 ) by CD4+ and CD8+ T lymphocytes using four colour flow cytometry . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In various models of acute liver injury , a positive amplification loop involving TNFalpha , IFNgamma , and IL 12 has been implied that involves Kupffer cells ( macrophages ) , hepatic lymphocytes and non parenchymatous liver epithelial cells ( NPECs ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found that C3H / HeCr mice produced up to 5 fold more serum TNFalpha and 66 % higher IFNgamma levels in response to i . v . ^^^ These results indicate that the altered TNFalpha / IL 6 ratio in C3H / HeCr mice , as compared to control CBA mice , as well as the increased IFNgamma level , may be responsible for the increased susceptibility to endotoxemia in that substrain . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , TNFalpha , IL 6 and IFNgamma productions were unaffected . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Fc domain integrity was assessed by immunoblotting , quantitatively comparing the binding of irradiated and non irradiated materials to cell surface Fcgamma receptors , and by employing quantitative RT PCR to study the kinetics of accumulation of mRNA for the immune modulatory cytokines IL 1alpha , IL 1beta , IL 4 , IL 8 , IFNgamma , and TNFalpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Perforin , FasL , TNFalpha , IL 1 , IFNgamma , and NO have been claimed as the effector molecules ; however , they , as a single agent , might explain only part of beta cell death in type 1 diabetes . ^^^ Combinations or synergism between IFNgamma and TNFalpha or IL 1beta are being revisited as the death effectors , and molecular mechanism explaining such a synergism was addressed in several recent papers . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , as shifting the immune response toward the Th 2 pattern ( IL 4 , IL 5 , IL 6 ) may benefit the fetus , whereas development of proinflammatory Th 1 cells ( secreting IL 2 , IFN g , TNF a ) may be harmful . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : Prolonged exposure ( 48 hours ) of corneal endothelium to IL 1 , IFNgamma , and TNF ( 100 ng / mL each ) resulted in induction of apoptosis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Interleukin 18 ( IL 18 ) is a cytokine capable of induction of IFNgamma , granulocyte monocyte colony stimulating factor ( GM CSF ) , TNFalpha and IL 1 in immunocompetent cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
To our surprise , nave T cells had a skewed Th1 / Tc1 cytokine secretion pattern with high amounts of IL 2 , IFNgamma and TNFalpha , but low amounts of IL 4 , IL 5 and IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The present article reviews the knowledge regarding the effect of pro inflammatory cytokines ( TNFalpha , IL 1beta , IL 6 , IL 2 , IFNgamma ) on the expression and the functionality of P gp at three major sites of drug absorption and disposition : the liver , the blood brain barrier , and the intestine . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Production of IFNgamma , TNFalpha and IL1beta , and chemokines , namely CXCL 5 , CXCL 9 , CXCL 10 , CXCL 11 , CCL 5 , and CCL 2 , by infiltrating leukocytes and signs of defective neovascularization were detected in tumours expressing h LAG 3 , m LAG 3 , and m IL 12 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Lipopolysaccharide or lipoteichoic acid mediated triggering of PBMCs incubated with IL 12 / IL 18 or IFNgamma led to an increased production of both TNFalpha and IL 6 , indicating the functionality of TLR 2 and TLR 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The role of immunomodulation in systemic anti fungal therapy has been proposed and recombinant cytokine therapy ( G CSF , GM CSF , IFN g , IL 1 , TNF a ) either alone or in combination with AmB have shown good results . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNFalpha in turn could replace the immature production of IL 12 , IL 18 and IFNgamma during URTI to induce an effective clearance of the viral infection and which could stimulate the maturation of Th 1 cytokine production in infancy . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The most useful appear to be TNFalpha antagonists ( oxpentifilline , infliximab , entanercept , onercept and thalidomide ) , anti alpha 4 integrin antibodies ( natalizumab ) , IL 10 , anti CD 4 , IL 6 , IL 12 , IL 18 , IFNgamma , IL 2R antibodies and others . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
They were able to produce a variety of immunomodulatory cytokines such as IFNgamma , TNFalpha , and MIP 1beta , but also GM CSF and G CSF when co incubated in culture with and without various stimuli . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Detection of CD3+ lymphocyte staining for spontaneous intracellular TNFalpha , IL 2 , IL 4 , IFNgamma , and IL 10 , was performed by multicolor flow cytometry . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The cytotoxic T lymphocyte ( CTL ) activity was determined by LDH method , the expression of TNF a and IFN g in tumors were assayed by RT PCR . ^^^ The expression levels of TNF a and IFN g mRNA were remarkably increased ( TNF a , F = 47 . 84 ; IFN g , F = 37 . 23 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Levels of PTHrP and five types of cytokines considered to be associated with cachexia , TNFalpha , IL 1beta , IL 6 , IFNgamma and LIF , were measured during routine blood examination and were compared with clinical findings . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNFalpha , IL 1 and IFNgamma decreased VEGF stimulated vasohibin expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The aim of this study was to evaluate whether there was any correlation between Helicobacter pylori associated diseases and ( 1 ) H . pylori virulence genes or ( 2 ) IL 1B , IL 1RN , IFN G , TNF A , IL 10 genetic polymorphisms . ^^^ IL 1RN intron 2 VNTR polymorphism ( PCR ) , IL 1B 31 C / T ( RFLP ) , the SNPs of IFN G ( +874 A / T ) , TNF A ( 1031 C / T , 857 C / T , 376 A / G , 308 A / G , 238 A / G ) , IL 10 ( 1082 A / G , 819 C / T , 592 A / C ) ( Taqman chemistry ) were studied . cagA , s 1 and m 1 vacA , were PCR amplified . ^^^ H . pylori infection was associated with TNF A 308 AG genotype , while IFN G +874 AA genotype was associated with cagA . ^^^ In conclusion , among host genetic factors contributing to H . pylori disease outcome , IFN G +874 AA genotype favors cagA positive infections , TNF A 857 TT duodenal ulcer while IL 10 819 TT intestinal metaplasia and NCGC . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Antibodies against murine IFNgamma , TNFalpha and IL 18 completely abolished the inhibitory effect of imiquimod on angiogenesis induced by murine L 1 sacroma cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
It is reported here that in two study populations , Canadian Aboriginal individuals have a higher frequency of cytokine single nucleotide polymorphisms favouring a low production of TNFalpha , IFNgamma and IL 10 and high production of IL 6 as compared to a Caucasian population . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Other mediators of T cell induced cytotoxicity , such as TNFalpha and IFNgamma may contribute to mucosal damage but are not required for the induction of mucosal damage in vivo . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokine regulation of PL 74 mRNA expression in term cytotrophoblasts showed that epidermal growth factor and IFNgamma increased PL 74 expression , but TGFbeta and TNFalpha had no effect . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Besides , after activation with PMA plus ionomycin , CD4+2E3 SLADR T cells produced IFNgamma and TNFalpha , although they did also in lower proportion than the whole CD4+2E3 population . ^^^ Most of the IFNgamma TNFalpha+ , IFNgamma+TNFalpha+ , IFNgamma+TNFalpha cells were CD8alpha+ and CD45RA , while IFNgamma TNFalpha cells showed a less differentiate phenotype . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Similarly , numbers of inflammatory leukocytes , cytokine expression of IL 6 , IL 12p40 , IFNgamma IL beta mRNA , and brain edema were significantly increased in TNF ( 0 / 0 ) mice as compared to WT animals . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
GFP ( + ) pDC 1 subset was less potent than GFP ( ) pDC2s in T allostimulation and production of tumor necrosis factor alpha ( TNFalpha ) , interferon alpha ( IFNalpha ) , and interleukin 6 ( IL 6 ) , while only pDC2s made IFNgamma and IL 12 p 70 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
During rejection , IFNgamma , IL 12 and IL 5 were significantly elevated over sham operated controls , whereas IL 2 , TNFalpha , IL 4 , IL 6 , IL 10 , IL 1beta and TGFbeta were unchanged . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFNgamma , TNFalpha ) and TH 2 paradigm ( i . e . ^^^ MS patients displayed a blunted response of IFNgamma ( P =0 . 03 ) whereas TNFalpha and IL 10 responses did not change . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Forty eight patients were analyzed for mutations in the IL1A , IL1B , IL 6 and TNFA genes , and 16 polymorphisms in 10 candidate cytokine genes ( IL1A , IL1B , IL1RN , TNFA , IL 2 , IL 4 , IL4R , IL 6 , IL 10 , IFNG ) were genotyped from all subjects . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Candidate gene and genome wide studies have localized genetic regions involved in the disease , such as the A1AR and CLCA 1 genes ( chromosome 1 ) , IL 1RN and DPP 10 ( 2q14 ) , HLA G and TNF a ( 6p21 ) , GPRA ( 7p14 ) , FceRI and GSTP 1 ( 11q13 ) , NOS 1 , IFNG , STAT 6 , VDR , and other genes ( 12q13 26 ) , PHF 11 and flanking genes ( 13q14 ) , AACT and PTGDR ( 14q ) , and ADAM 33 ( 20p13 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PGE 2 , PGI 2 , and NO levels were assayed in culture media from non tumorigenic mouse lung epithelial cell lines , their spontaneous transformants and mouse lung tumor derived cell lines , before or after exposure to the cytokines TNFalpha , IFNgamma and IL1beta , alone and in combination . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFNgamma and TNFalpha synergistically induce neurite outgrowth on PC 12 cells . ^^^ In addition , because the synergism between TNFalpha and IFNgamma is well known , this study investigated whether or not a mixture of IFNgamma and TNFalpha might augment neurite outgrowth on PC 12 cells . ^^^ In conclusion , it was shown that IFNgamma has the potential to form neurites , and TNFalpha can enhance this ability . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RT PCRs for TNFalpha , IFNgamma , and IL 4 and immunohistochemical staining were performed to identify infiltrating cells and cytokines . ^^^ Cytometric bead array was used to measure the levels of TNFalpha , IFNgamma , and IL 4 protein . ^^^ TNFalpha , IFNgamma , and IL 4 mRNA and protein were upregulated during the evolution of ARN in HSV 1 infected contralateral eyes compared with levels in control subjects . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Studies on the mechanism of action showed that low doses of targeted IFNgamma could activate tumor necrosis factor ( TNF ) dependent antitumor mechanisms , whereas high doses of either targeted or nontargeted IFNgamma induced soluble TNF receptor shedding in circulation , a known counterregulatory mechanism of TNF activity . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Compared to younger animals , older calves showed significantly less fever and lower TNFalpha levels and less virus specific IFNgamma release . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The T cells generated upon stimulation with DCs subjected to AdV infection , and subsequently treated with TNF alpha , exhibited tumor antigen specificity , but accompanied by reduced proliferation and IFNgamma production and increased IL 10 production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The TCRgamma / delta+ anti ergotypic T cells secreted IFNgamma and TNFalpha in response to activated T cells ; the TCRalpha / beta+ T cells proliferated but did not secret detectable cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In particular , it has been shown that the expression of MyoD , a muscle specific transcription factor , is down regulated by cytokines such as TNFalpha and IFNgamma in a NF kappaB dependent posttranscriptional manner . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Canine specific primers for IL 1beta , IL 2 , IL 4 , IL 6 , IL 8 , IL 10 , IL 12 , TNFalpha , IFNgamma and TGFbeta 1 were used . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We demonstrated that both IFNgamma ( + ) and , CD 4 and CD 8 TNFalpha ( + ) T cells specifically respond to FIV antigens . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In liver LPS+FB ( 1 ) attenuated the expression TNFalpha and IFNgamma compared to LPS alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PG 490 88 treatment exhibited significant inhibition of intragraft m RNA levels of iNOS , IL 6 , and perforin and marginal downregulation of IL 2 , IFNgamma , IRF 1 , TNFalpha , and TGFbeta . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
TNFalpha , IFNgamma and IL 10 gene polymorphisms in a sample of Sicilian patients with coeliac disease . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , the majority of central memory CD 4 T cells did not secrete TNFalpha or IFNgamma and thus would not be identified by techniques based on their secretion . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Using real time reverse transcription polymerase chain reaction , treatment of betaTC 3 cells with IL 1beta + TNFalpha + IFNgamma induced a significant seven fold increase in PANDER mRNA expression ( n = 3 ; p < 0 . 01 at 24 h , p < 0 . 05 at 48 h ) , while IFNgamma alone caused a 3 . 2 fold increase ( n = 3 ; p < 0 . 01 at 24 h ) compared to unstimulated and time matched vehicle controls . ^^^ Under those conditions , a similar up regulation was also observed in mouse islet cells , with increases in PANDER mRNA of 5 . 9 fold and 5 . 0 fold after treatment with IL 1beta + TNFalpha + IFNgamma or IFNgamma alone . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : We found that patients with IgA antibodies towards Chlamydia lipopolysaccharide ( LPS ) had elevated levels of IFNgamma ( P = 0 . 048 ) , IL 10 ( P = 0 . 029 ) , TNFalpha ( P = 0 . 009 ) and sE selectin ( P = 0 . 045 ) , while Chlamydia LPS IgG seropositivity predicted elevated levels of IL 10 ( P = 0 . 013 ) . ^^^ CONCLUSIONS : In summary , we demonstrated an association between Chlamydia LPS IgA seropositivity and elevated levels of IFNgamma , IL 10 , TNFalpha , sVCAM 1 and sE selectin in CHD patients that might indicate persistent Chlamydia infection and a proinflammatory state . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Both twins also had significantly elevated levels of IFNgamma , TNFalpha , IL 4 and IL 10 in the serum , compared to the controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Whereas single proinflammatory mediators were unable to induce the maturation of neonatal DCs , various combinations of IFNgamma , CD40L , TNFalpha , LPS and antigens , induced the maturation of neonatal DCs documented by up regulation of HLA DR , CD 83 and CD 86 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Production of IFNgamma , IL 6 , TNFalpha , MIP 1alpha , and MIP 2 , inducible NOS ( iNOS ) , COX 2 , and nitrotyrosine poly ADP ribose ( PAR ) were also less in CII treated GITR / mice . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Non encephalitogenic APL that induce production of the ' regulatory ' cytokines IL 10 and / or TGFbeta and concomitant low levels of the ' proinflammatory ' cytokines IFNgamma and TNFalpha modulated relapsing EAE but were far less effective than the ' proinflammatory ' wild type MOG 8 21 peptide . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The obtained results will direct our further investigations toward obtaining mAbs for receptors for TNFalpha and IFNgamma , transfection of suppressor gene into tumor cell cultures and genes for IL 2 and TNFalpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NK cells are activated and increase their cytotoxic potential and modulate their cytokine production after treatment with IFNgamma , IL 12 , TNFalpha and IL 2 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
T lymphocytes proliferate in response to beryllium antigens and combined with macrophages produce numerous epithelioid granulomas with the release of inflammatory cytokines ( IFNgamma , IL 2 , TNFalpha and IL 6 ) and growth factors . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In the MLNs , on the other hand , the folate supplemented diet failed to influence any age related increase in interleukin ( IL ) 2 , tumor necrosis factor alpha and IFNgamma following stimulation but did result in a significantly increased production of IL 4 ( P < . 05 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Fourteen cytokine genes were identified as candidates : gamma interferon ( IFNgamma ) ; interleukin ( IL ) 1 alpha ( IL 1alpha ) ; IL 1 beta ( IL 1beta ) ; IL 1 receptor ( IL 1R ) ; IL 1R antagonist ( IL 1RA ) ; IL 2 ; IL 4 ; IL 4 receptor alpha ( IL 4Ralpha ) ; IL 6 ; IL 10 ; IL 12B ; TGF beta 1 ( TGF beta 1 ) ; and TNF alpha ( TNF alpha ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
These observations were also generally supported by the results obtained using bioassays for IL 1 and IL 6 and ELISA for IL 1alpha , IL 1beta , IL 6 , TNFalpha , and IFNgamma . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Inhibitors for nuclear factor kappa B ( NFkappaB ) , parthenolide , and Bay 11 7085 , and an inhibitor of p 38 , SB 202190 , inhibited TNFalpha and IFNgamma induced production of CCL 17 by HaCaT KC . ^^^ Roxithromycin ( RXM ) , a 14 membered ring macrolide , suppressed CCL 17 production by HaCaT KC induced by IFNgamma and TNFalpha . ^^^ RXM partially suppressed p 38 phosphorylation and NFkappaB driven luciferase activity induced by TNFalpha and IFNgamma . ^^^ Degradation of inhibitor of nuclear factor kappa B ( IkappaB ) alpha upon stimulation with IFNgamma and TNFalpha was not affected by the addition of RXM . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Bcl xL / Bax ratio is altered by IFNgamma in TNFalpha but not in TRAIL induced apoptosis in colon cancer cell line . ^^^ In HT 29 D4 cells , a model of human colon carcinoma , IFNgamma presensitization is essential to induce an apoptotic response to TNFalpha whereas it only slightly enhances TRAIL induced apoptosis . ^^^ To compare the transcriptional profiles induced by TNFalpha and TRAIL and their regulation by IFNgamma , we optimized a cDNA array analysis on targeted signaling pathways and confirmed the gene expression modulations by comparative RT PCR . ^^^ In contrast , IFNgamma pretreatment prevented Bcl xL gene up expression in TNFalpha stimulated cells and allowed cytochrome c release . ^^^ Thus , we hypothesize that the Bcl xL / Bax ratio can block the apoptotic response in TNFalpha stimulated cells but allows cell death initiation when it is altered by a crosstalk between IFNgamma presensitization and TNFalpha induced signalings . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Of the pro inflammatory cytokines measured , including IFNgamma , TNFalpha , IL 12p70 , and MCP 1beta , none were significantly different in the sera of mice with exacerbated parasitemia compared to uninfected controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IL 12 and IFNgamma were expressed by CD40L engineered DCs , while TNFalpha matured DCs lacked IFNgamma and exhibited low IL 12 expression . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Evaluation of cytokine polymorphisms ( TNFalpha , IFNgamma and IL 10 ) in Down patients with coeliac disease . ^^^ AIMS : To evaluate a possible correlation between TNFalpha , IFNgamma and IL 10 genotype polymorphisms with the susceptibility to coeliac disease in Down syndrome patients . ^^^ METHODS : Single nucleotide polymorphisms of TNFalpha ( 308G > A promoter region ) , IFNgamma ( +874T > A promoter region ) and IL 10 ( 1082G > A promoter region ) have been studied in 10 Down patients with coeliac disease , in 40 Down patients without coeliac disease and in 220 healthy controls . ^^^ CONCLUSIONS : Single nucleotide polymorphisms of IFNgamma and IL 10 do not play a role in predisposing Down syndrome patients to coeliac disease , while the TNFalpha 308 allele could be an additional genetic risk factor for coeliac disease in trisomy 21 . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFNgamma and its transcriptional target tumor necrosis factor ( TNF ) related apoptosis inducing ligand ( TRAIL ) are two major effector molecules of activated CTLs and NK cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pretreatment or co treatment of human PBMC or murine lymphnode cells with EGCG significantly reduced SEB induced proliferation and IL 2 , IFNgamma , and TNFalpha production . ^^^ Culture supernatant from PBMC stimulated in the presence of EGCG failed to increase the permeability of T 84 epithelial cell monolayers : a finding consistent with the reduced IFNgamma and TNFalpha production by SAg+EGCG treated PBMC . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Pretreatment with polymyxin B prevented against acute pancreatitis induced lung injury and the otherwise occurring increases in TNF alpha , IL 1beta , MCP 1 and IL 10 , as well as against the decreases in IL 2 , IFNgamma and TIMP 1 , decreased protease activity and down regulation of CD 31 , CD 54 and CD62L on recruited neutrophils and macrophages in BALF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Bromelain induced in all 24 individuals a significant increase of the macro phage / monocyte associated cytokines interleukin ( IL ) 6 , granulocyte macrophage colony stimulating factor ( GM CSF ) , tumour necrosis factor alpha ( TNFa ) ( p < 0 . 01 ) as well as of the type 1 cytokine gamma interferon ( IFNgamma ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The neurotransmitter dopamine on its own increased significantly TNFalpha and IL 10 secretion by resting normal human T cells , and induced approximately 5 fold elevation of the corresponding mRNA ' s , without affecting IFNgamma and IL 4 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Classically , this disease was viewed in terms of type 1 versus type 2 immunity : the type 1 cytokines IFNgamma and TNFalpha promoting disease , whereas an IL 4 dominated , type 2 response was protective . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
By studying their pattern of expression , we found that three of them were not expressed in peripheral macrophages , even after stimulation with IFNgamma , TNFalpha or IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : AqH from noninflammatory control subjects and patients with idiopathic , Fuchs ' heterochromic cyclitis ( FHC ) , and herpes viral or Behet ' s uveitis were analyzed for IL 1beta , 2 , 4 , 5 , 7 , 8 , 10 , 12 , 13 , 15 , TNFalpha , IFNgamma , CCL 2 ( MCP 1 ) , CCL 5 ( RANTES ) , CCL 11 ( Eotaxin ) , TGFbeta 2 , and CXCL 12 ( SDF 1 ) , using multiplex bead immunoassays . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Real time RT PCR assays revealed that the mRNA levels for IFNgamma , TNFalpha , and IL 8 rose over the first few days of TB pleuritis and then declined over the 9 days of the study . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study , we investigated the effects of poly ( 1 : C ) on the production of chemokines ( IL 8 , RANTES , and eotaxin ) , Type 1 IFNs ( IFNalpha and IFNbeta ) , Th 1 cytokines ( IL 12 and IFNgamma ) , and pro inflammatory cytokines ( TNF alpha and IL 1beta ) by human nasal mucosa derived fibroblasts . ^^^ However , eotaxin , IL 1beta , TNF alpha , IFNalpha , IFNgamma , and IL 12 were not secreted from nasal fibroblasts stimulated with poly ( 1 : C ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The suspension bead array yielded statistically significant cytokine protein expression increases for IL 6 , IL 10 , IFNgamma and TNFalpha at both 2 h and 24 h post treatments , while significant expression at 24 h post treatment only was noted for IL 1beta , IL 5 , IL 12 and GM CSF . ^^^ Suspension bead array provided the greatest range of detection , revealing subtle increased expression of GM CSF , IL 1beta , IL 5 , IL 10 , TNFalpha and IFNgamma at 24 h post treatment , not detected by antibody array or multiple antibody Western blot . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The production of IL 4 , IFNgamma , and TNFalpha by CD 8 T cells was measured by intracytoplasmic detection using flow cytometry in both resting and stimulated cells with a phorbol ester . ^^^ No statistically significant differences were found in IFNgamma and TNFalpha levels at month 3 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We found a significant increase of IFNgamma and TNFalpha production ( p < 0 . 01 ) and a significant decrease of IL 10 production ( p < 0 . 01 ) in cultures of lymphomonocytes taken from patients with AMI and UA compared with SA patients and controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Following intestinal T cell activation in predisposed individuals , different inflammatory mechanisms are triggered under the control of the cytokine balance including those with a pro inflammatory Th 1 pattern such as IFNgamma , TNFalpha , IL 15 and IL 18 ; and regulatory cytokines such as TGFbeta and IL 10 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In addition , the nonspecific stimulation of IFNgamma , TNFalpha , and IL 12 secretion by MLN cells was significantly higher in the CM group as compared to the other groups . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Supernatant samples were analysed by ELISA for TNFalpha , IFNgamma and IL 10 production . ^^^ This was accompanied by increased medium levels of TNFalpha , IFNgamma and IL 10 , which suggest that either CD 4 ( + ) CD 25 ( + ) cells , macrophages , or both , down regulate production of both Th 1 and certain Th 2 cytokines . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
There was a significant increase in IFNgamma , IL 2 , IL 4 , TGFbeta , and TNFalpha production by CD 8 T cells and IFNgamma and TNFalpha production by CD 4 T cells in BAL from transplant patients compared with controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CXCL 13 mRNA and protein expression was induced by TNFalpha and IL 1beta but inhibited by IL 4 and IFNgamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In contrast , the synergistic induction of CD 38 by the combination of TNFalpha with IFNgamma or IFNbeta , but not with IL 1beta or IL 13 , was completely insensitive to the GC inhibitory effects . ^^^ We also found that TNFalpha and IFNgamma impaired GC responsiveness by inhibiting steroid induced both 1 ) GRalpha DNA binding activity and 2 ) GC responsive element ( GRE ) dependent gene transcription . ^^^ Although levels of the GC receptor ( GR ) alpha isoform remained unchanged , expression of GRbeta , the dominant negative GR isoform , was synergistically increased by TNFalpha and IFNgamma with a GRalpha / GRbeta ratio of 1 to 3 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In particular , an important increase in the spontaneous ex vivo secretion of TNFalpha , IL 6 and IFNgamma as well as IL 2 and IL 10 was frequently observed prior to the fourth vaccination . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : We compared whole blood stimulatory capacity for pro ( TNFalpha , IFNgamma ) and anti inflammatory cytokines ( IL 10 ) as well as hypothalamo pituitary adrenal ( HPA ) axis function in 15 MS patients with marked fatigue and 15 patients without fatigue as determined by the Fatigue Severity Scale ( FSS ) . ^^^ RESULTS : Proinflammatory cytokines were significantly higher ( TNFalpha : 478 . 9 5 228 . 2 pg / ml , p = 0 . 01 ; IFNgamma : 57 . 6 5 27 . 8 pg / ml ; p = 0 . 01 ) in MS patients with fatigue . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We used real time PCR to quantify cytokines that contribute to innate and adaptive immune responses , including IFNalpha , IFNgamma , TNFalpha , TGFbeta ( 1 ) , IL 2 , IL 4 , IL 8 , IL 10 , and IL 12p40 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
We show that G CSF enhances MS autoreactive T cell line adhesion to the ECM proteins collagen 4 and fibronectin as effectively as the proinflammatory IFNgamma and TNFalpha , known to exacerbate MS symptoms . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
By using specific cytokine inhibitors we were able to show that endogenous IL 1 , but not IL 18 and TNFalpha was required for IFNgamma and IL 10 release upon stimulation with A . fumigatus hyphae , whereas conidia induced IFNgamma stimulation is independent of these cytokines . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Although the disease was stable , there had been a significant increase of proinflammatory cytokines such as TNFalpha and IFNgamma in the CSF and serum , with no significant changes of IL 12 and IL 10 production . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The study demonstrated the ability of L . acidophilis to activate expression of the genes of pro inflammatory cytokines ( IFNgamma , IL 6 , 12 , and TNFalpha ) , to induce production of IFNalpha and IFNgamma , and increase interferon production , with additional stimulation of lymphoid organ cells with specific inductors in vitro . ^^^ The study detected immunocorrecting effect of L . acidophilis by the example of experimental shigellous infection , demonstrated intensification of the prolipherative activity of immunocompetent T cells , induction of the expression of the genes of pro inflammatory cytokines ( IFNgamma , IL 1beta , 6 , 12 , and TNFalpha ) , but not IL 4 and 10 , increase of synthesis of IFNalpha and IFNgamma by the cells of Peyer ' s plaques , the splen and thymus . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Here we show that a mouse lymph node ( LN ) derived FRC cell line , BLS 4 , expresses a transmembrane chemokine , CXC chemokine ligand ( CXCL ) 16 , in response to tumor necrosis factor alpha ( TNFalpha ) and IFNgamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The Death Associated Protein 3 ( DAP 3 ) , a GTP binding constituent of the small subunit of the mitochondrial ribosome , is implicated in the TNFalpha and IFNgamma apoptotic pathways of the cell and is involved in the maintenance of the mitochondrial network . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Intrahepatic and peripheral blood CD8+T cells were obtained from 32 HCV chronically infected patients and analysed by flow cytometry for surface markers of differentiation , IFNgamma and TNFalpha production , degranulation capacity and perforin content , after CD 3 triggering . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
METHODS : Highly purified ( > 98 % ) CD4+ and CD8+ T cells of HLA B 27 positive AS patients ( n = 13 ) , healthy HLA B 27 positive ( n = 7 ) and negative controls ( n = 9 ) were stimulated for 6h with PMA / Ionomycin and , after fixation , stained for surface markers CD45RA and CD 27 and cytokines TNFalpha , IFNgamma , IL 4 and IL 10 . ^^^ RESULTS : CD27+ CD45RA memory CD8+ T cells of HLA B 27 positive subjects showed a significantly lower percentage of TNFalpha ( median 71 . 4 % ) and IFNgamma production ( median 69 . 7 % ) than HLA B 27 negative controls ( TNFalpha 85 . 1 % ; p < or = 0 . 027 ; IFNgamma 82 . 7 % , p < or = 0 . 026 ) . ^^^ A similar result was also detected in CD 27 CD45RA+ effector CD8+ T cells of which 43 . 2 % produced TNFalpha and 66 . 3 % IFNgamma in HLA B 27 positive subjects , respectively , compared to 75 . 6 % TNFalpha and 84 . 4 % IFNgamma producing T cells in HLA B 27 negatives ( p < or = 0 . 045 and p < or = 0 . 062 , respectively ) . ^^^ For all CD4+ T cell subsets no significant differences between HLA B 27 positive and HLA B 27 negative donors were observed , regarding neither the frequency of IFNgamma , TNFalpha , IL 4 or IL 10 producers nor the coexpression of IFNgamma and IL 4 in memory subsets . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
CO inhibited intragraft upregulation of Th 1 type cytokines ( IL 2 , IFNgamma ) , proinflammatory mediators ( IL 1beta , TNFalpha , IL 6 , COX 2 ) , and adhesion molecule . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Second , local immune response was estimated by TNFalpha , IFNgamma , and iNOS mRNA expression in the grafts by quantitative PCR , which revealed 20 to 40 fold increases at POD 28 after allotransplantation . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study examined the ability of IFNgamma and TNFalpha to stimulate a better invasiveness in B 16 murine melanoma cells , and investigated whether this enhanced ability was related to a higher expression of protease activities , such as urokinase plasminogen activator ( uPA ) and its receptor ( uPAR ) , and matrix metalloproteinases 2 and 9 ( MMP 2 , MMP 9 ) . ^^^ We found that murine melanoma cells enhanced their lung colonizing potential in vivo and invasiveness through Matrigel coated filters upon costimulation with IFNgamma and TNFalpha ; neither IFNgamma nor TNFalpha alone , at the dose used in the experiments , was able to elicit a change in the invasive / metastatic efficiency of melanoma cells . ^^^ The invasive phenotype of murine melanoma cells stimulated with IFNgamma and TNFalpha was characterized by an enhanced uPA / uPAR and MMP 9 expression : TNFalpha promoted MMP 9 mRNA expression and pro MMP 9 protein secretion , and the costimulation with IFNgamma and TNFalpha was required to potentiate the expression of mRNA and protein for uPAR , and to induce a redistribution of uPA from the soluble to the cell body associated form . ^^^ Both monoclonal antibodies , anti uPAR and anti MMP 9 , caused a significant reduction of invasiveness in IFNgamma / TNFalpha stimulated melanoma cells . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Treatment with the LXR activator T 0901317 reduced this increase of IFNgamma , TNFalpha , and IL 2 in a concentration dependent manner with a maximum at 1 micromol / L T 0901317 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
IFNgamma added prior to infection of mouse peritoneal macrophages with IgA opsonized bacilli resulted in a synergistic increase of nitric oxide and TNFalpha production and a 2 3 fold decrease in bacterial counts . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Intestinal epithelial cells , HCT 8 , were stimulated by IL 1beta , IFNgamma and TNFalpha each alone or in combination ( Cytomix ) . ^^^ In contrast , the activity of ubiquitin proteasome system was stimulated by IFNgamma and Cytomix ( 155 , 160 versus 100 , P < 0 . 05 , respectively ) but remained unaffected by IL 1beta and TNFalpha . ^^^ In conclusion , lysosomal , Ca ( 2+ ) activated and constitutive proteasome activities were not affected by IL 1beta , IFNgamma and TNFalpha alone or in combination , in HCT 8 cells . ^^^ These results suggest that IFNgamma , but not IL 1beta and TNFalpha , increases immunoproteasome , which might contribute to enhanced antigen presentation during inflammatory bowel diseases . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The models identified capture many of the known signaling pathways involved in cytokine release and predict potentially important novel signaling components , like p 38 MAPK for G CSF release , IFNgamma and IL 4 specific pathways for IL 1a release , and an M CSF specific pathway for TNFalpha release . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Spleen cells , particularly CD4+ T cells among them produced IL 2 , IFNgamma and TNFalpha in response to the killed BCG or purified protein derivatives . ^^^ CD8+ T cells including cytotoxic T lymphocytes produced IFNgamma and TNFalpha . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
A minor Th 1 function would favor infectious processes by a reduction in TNF alpha and IFNg . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Perforin and Fas induced by IFNgamma and TNFalpha mediate beta cell death by OT 1 CTL . ^^^ It was also prevented by pre incubation with anti tumor necrosis factor alpha ( anti TNFalpha ) antibody or by blocking IFNgamma responsiveness through expressing a dominant negative IFNgamma receptor . ^^^ Perforin deficient CTL produced IFNgamma and TNFalpha that was shown to directly induce islet Fas expression during the assays . ^^^ This suggests that Fas deficiency , SOCS 1 overexpression and blockade of IFNgamma and TNFalpha all protect beta cells from residual cytotoxicity of perforin deficient CTL by blocking Fas upregulation . ^^^ However , in the absence of perforin , the Fas / FasL pathway provides an alternative mechanism dependent on islet cell Fas upregulation by cytokines IFNgamma and TNFalpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Cytokines influencing Th 1 and Th 2 cell differentiation , including IFNgamma , IL 2 , IL 4 , IL 5 , IL 10 and IL 12p40 , as well as dendritic cell regulating cytokines IL 1alpha , IL 1beta , IL 6 , LIF , GM CSF and TNFalpha were also expressed similarly regardless of fertility status . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
However , the present study failed to detect signs of inflammation in the cerebrospinal fluid samples that is , pleocytosis , oligoclonal immunoglobulin G bands , increased expression of IFNgamma , TNFalpha or interleukin 10 , or increased levels of nitric oxide oxidation products . ^^^ Induction of systemic IFNgamma production may still be relevant in neuroinflammation associated with treatment with anti TNFalpha . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The results revealed that the mRNA expressions of IL 1beta , IL 6 , IFNgamma , TNFalpha , and 5 HTT were higher in the depressed patients than those of the healthy controls . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NK cell activity , perforin and CD 81 expression , the IFNgamma , TNFalpha , IL 2 ( Th 1 ) and IL 4 , IL 6 , IL 10 ( Th 2 ) production of LPS stimulated monocytes and PMA + ionomycine stimulated lymphocytes were measured by flow cytometry . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Loss of IL 12 decreased but did not abolish the hepatic expression of IFNgamma , displayed a remarkable increase in expression of TNFalpha , IFNalpha , IFNbeta and decreased expression of IL 4 and IL 5 . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
While no variations were seen in serum IL 12 or TNFalpha levels , a high IFNgamma secretion was detected from Day 8 , concomitant to IFNalpha , followed by IL 10 . ^^^ Specific Th 1 and CD 8 responses were detected on Day 28 , with high IFNgamma / TNFalpha ratios . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In this study we investigated the expression levels of Dpl and PrP on lymphocytes , monocytes and neutrophils ( PMNs ) isolated from bovine blood and incubated ( 2 and 18 h ) with TNFalpha , IL 1 , IL 2 , IL 8 , C5a , IFNgamma , anti PrP , and anti Dpl antibodies by flow cytometry . ^^^ These cells overexpressed PrP when incubated with TNFalpha and IFNgamma , and both PrP and Dpl when incubated with C5a ; incubation with TNFalpha , IL 8 and IFNgamma led to down regulation of Dpl . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
BLS KETc 1 also increased the levels of transcription of TNF alpha , IL 2 and IFNgamma in Peyer ' s patches , and in spleen , only increased TNF alpha was observed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
At the same time , some pro inflammatory cytokines such as IFNgamma and TNFalpha also increased , but the intestinal homoeostasis was maintained and no tissue damage was observed . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This analysis revealed that mRNAs for tumor necrosis factor alpha , IFNgamma , Krt 16 , CD 2 , IL 23A , IL 12B , and vascular endothelial growth factor are overexpressed in the `` average ' ' psoriatic lesion in a majority of patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Specifically , OFQ / N enhanced TNFalpha and IFNgamma transcripts in the spleen when injected prior to SEA challenge . ^^^ Moreover , mice lacking the OFQ / N precursor gene showed diminished TNFalpha and IFNgamma mRNA induction in the spleen following SEA challenge . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In a murine lipopolysaccharide induced liver failure model , the production of tumor necrosis factor alpha ( TNFalpha ) , IFNgamma , IL 1 beta , ALT and AST were effectively reduced by Man liposome complexes . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
AIRmax mice were more susceptible than AIRmin to acute colitis induced by ingestion of dextran sodium sulfate showing a 3 fold higher disease activity index and presenting an intense inflammatory infiltrate in the base of colon crypts as well as elevated expression of IL 1beta , TNFalpha , IFNgamma and IL 6 mRNA in colon tissue . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Compared with healthy controls , CMV stimulated lymphocytes from HAART recipients had lower 3H thymidine incorporation ; lower IFNgamma and TNFalpha production ; higher CD4+ CD 27 CD 28 and CD8+ CD 27 CD 28 frequencies ; lower CD4+ CD25hi ; and higher FoxP 3 expression in CD8+ CD25hi cells . ^^^ CMV specific proliferation correlated with higher IFNgamma , TNFalpha and IL 10 levels and higher CD4+ perforin+ and CD8+ perforin+ frequencies . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Increased levels of TNFalpha and IFNgamma , observed post ECP in monocytes and lymphocytes , respectively , are thought to further contribute to the proposed anti tumour reaction seen in CTCL . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Despite the controversial discussion of cachexia inducing mechanisms it is clear that proinflammatory cytokines , such as TNFalpha , IFNgamma , IL 1 , IL 6 and IL 8 , are linked to all pathways that induce cachexia . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
PATIENTS AND METHODS : The human TH 1 / TH 2 cytometric bead array ( CBA ) kit was used to investigate IL 2 , IFNgamma , TNFalpha , IL 4 , IL 5 and IL 10 in a control group and in 13 patients . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
In all patients , the cytokines IL 1alpha , IL 1beta , IL 1ra , TNFalpha , IFNgamma , IL 2 , and TGFbeta were investigated using specific antibodies . ^^^ The main difference was the absence of IFNgamma and TNFalpha in TMJ CPA patients and a stronger TGFbeta and IL 1alpha expression . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
With regard to proinflammatory cytokine ( IFNgamma and TNFalpha ) , we found that TNFalpha was mainly produced by NC bacteria , while C bacteria were able to elicit mainly IFNgamma . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The previously reported increase of cytokine ( TNFalpha , IL 1alpha and IL 6 ) production by monocytes macrophages , and the release of IL 2 , IL 4 and IFNgamma by T lymphocytes , may play a role in the initiation of the humoral immune response in CSF . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The aim of this study was to evaluate whether imidazolines RX 871024 and efaroxan protect beta cells against death in the presence of a combination of the cytokines IL 1beta , IFNgamma , and TNFalpha . ^^^ Our data indicate that a combination of pro inflammatory cytokines IL 1beta , IFNgamma , and TNFalpha , conditions modelling those that take place in type 1 diabetes , induces pancreatic beta cell death that does not directly correlate with NO production and can not be counteracted with imidazoline compounds . . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
Mice lacking both TNFalpha receptors show increased constitutive expression of IFNgamma : a possible reason for lack of protection from fumonisin B 1 hepatotoxicity . ^^^ It appears that in the absence of TNFalpha signaling other apoptotic pathways become operative ; particularly the increase of IFNgamma , Fas and IL 18 may compensate for the loss of TNFalpha effects . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
This study documents a novel mechanism to keep TNFalpha levels low with stimuli that cause large fluctuations in IFNgamma release . ^^^ TNFalpha and IFNgamma production was assessed at both the mRNA and protein levels . ^^^ RESULTS : By 72h with IL 15 , IFNgamma concentrations increased markedly , while TNFalpha remained low . ^^^ With IL 12 supplementation , IFNgamma increased 10 fold , while TNFalpha remained low . ^^^ Initially , IELs had similar amounts of transcripts for IFNgamma and TNFalpha , indicating equivalent synthetic rates . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
RESULTS : We developed real time PCR based detection assays for several immune related transcription factor and cytokine genes from deer mice that permit the profiling of CD4+ helper T cells , including markers of Th 1 cells ( T bet , STAT 4 , IFNgamma , TNF , LT ) , Th 2 cells ( GATA 3 , STAT 6 , IL 4 , IL 5 ) and regulatory T cells ( Fox p 3 , IL 10 , TGFbeta 1 ) . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
The preliminary testing of TNF and IFNgamma secretion examined in supernatants of effector cells showed impaired levels of both cytokines in RA and normal levels in RARS in accordance with the findings achieved in alloreactivity analysis . ^^^
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01579 and P01375	Pubmed	SVM Score :0.0
NA