| Interacting proteins: P01266 and P13667 |
Pubmed |
SVM Score :0.0 |
| We have previously demonstrated that several endoplasmic reticulum ( ER ) proteins , including BiP , ERp 72 , grp 94 , and protein disulfide isomerase , bind to a denatured thyroglobulin ( Tg ) affinity column and can be specifically eluted by ATP ( Nigam , S . ^^^ Using chemical cross linking , we now demonstrate that BiP , ERp 72 , and grp 94 associate with Tg in two types of cultured thyroid cells , FRTL 5 and PCC 13 . ^^^ Whereas BiP could be coimmunoprecipitated with anti Tg antibodies in the absence of cross linking , only trace amounts of ERp 72 and grp 94 were coimmunoprecipitated . ^^^ Likewise , in both cell types , anti BiP antibodies were able to coimmunoprecipitate Tg in the absence of cross linking , though ERp 72 and grp 94 were only minimally present . ^^^ In contrast , after cross linking , there was a large increase in the amount of ERp 72 and grp 94 that coimmunoprecipitated with anti Tg antibodies , although there was only a slight increase in BiP . ^^^ |
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| Interacting proteins: P01266 and P13667 |
Pubmed |
SVM Score :0.0 |
| Furthermore , coimmunoprecipitation studies after antimycin A treatment demonstrated that Tg stably associates with BiP , grp 94 , and ERp 72 . ^^^ |
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| Interacting proteins: P01266 and P13667 |
Pubmed |
SVM Score :0.0 |
| By coimmunoprecipitation in rat thyroid cells , we were able to demonstrate that BiP , grp 94 , ERp 72 , and grp 170 , four proteins believed to function as specific molecular chaperones , complex with Tg during its maturation . ^^^ BiP , grp 94 , and ERp 72 were also found to associate with Tg in cells in which misfolding was induced by perturbing ER calcium stores . ^^^ |
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| Interacting proteins: P01266 and P13667 |
Pubmed |
SVM Score :0.0 |
| Counteracting the Tg induced rise in cytoplasmic calcium activity by preloading cells with the chelator BAPTA AM did not influence erp 72 expression significantly , suggesting that the activation of erp 72 expression resulted from the depletion of ER calcium stores and not from the corresponding increase in cytoplasmic calcium activity . ^^^ |
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| Interacting proteins: P01266 and P13667 |
Pubmed |
SVM Score :0.0 |
| Transient Tg exposure of neurons induced a marked rise in mRNA levels ( 7 fold and a 21 fold increase in erp 72 and HO 1 mRNA levels ; P < 0 . 001 ) . ^^^ |
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| Interacting proteins: P01266 and P13667 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01266 and P13667 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01266 and P13667 |
Pubmed |
SVM Score :0.0 |
| NA |
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