| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| In conclusion , the recognition of RAMP 1 and 2 as mediators of CRLR expression as a CGRP or ADM receptor has been extended , with evidence that endogenous RAMP 2 is sufficient to reveal an ADM receptor in UMR 106 cells . ^^^ Inhibition of RAMP 2 evoked ADM receptor expression by RAMP 1 and generation of a CGRP receptor is consistent with competitive interactions of the different RAMPs with rCRLR . . ^^^ |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| In this study it is shown that rat CRLR cDNA specifies a CGRP receptor when co transfected with RAMP 1 cDNA and an adrenomedullin receptor when co transfected with either RAMP 2 or RAMP 3 cDNA in human embryonic kidney 293 cells . 3 . ^^^ CGRP binding correlates well with RAMP 1 mRNA levels ( R=1 . 0 , P=0 . 007 ) , adrenomedullin binding shows a tendency to vary with RAMP 2 mRNA levels ( R=0 . 85 , P=0 . 14 ) and total binding is correlated with CRLR mRNA levels ( R=0 . 94 , P=0 . 03 ) . ^^^ |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| The displacing potencies of CGRP and CGRP [ 8 37 ] were very weak . mRNAs for AM as well as calcitonin receptor like receptor and receptor activity modifying protein 2 which form a receptor specific to AM , were highly expressed in the microvessels . ^^^ |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| Receptor activity modifying proteins ( RAMPs ) determine the ligand specificity of CRLR : coexpression of CRLR and RAMP 1 results in a CGRP 1 receptor , whereas coexpression of CRLR and RAMP 2 or 3 results in an ADM receptor . ^^^ |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| We recently detected the mRNA encoding for RAMP 2 , CRLR , and the CGRP receptor RDC 1 in rat lung . ^^^ |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| Our results also show that the three RAMPs lie close to the peptide binding pocket within the CRLR RAMP heterodimers , since ( 125 ) 1 AM and ( 125 ) 1 CGRP were incorporated in RAMP 2 , 3 , and 1 , respectively . ^^^ |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| Calcitonin receptor like receptor ( CRLR ) constitutes either a CGRP receptor when complexed with receptor activity modifying protein 1 ( RAMP 1 ) or an adrenomedullin receptor when complexed with RAMP 2 or RAMP 3 . ^^^ |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| As expected CRLR RAMP 1 coexpression conferred sensitivity to CGRP , whilst association of RAMP 2 or RAMP 3 with CRLR conferred high affinity ADM binding . ^^^ |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| In the present study , we examined the expression of the CGRP receptor activity modifying proteins ( RAMP 1 , RAMP 2 and RAMP 3 ) and receptor component protein ( RCP ) in human brain astrocytes ( AST ) , cerebromicrovascular endothelial ( EC ) and smooth muscle ( SMC ) cells in culture . ^^^ |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| Receptor activity modifying proteins ( RAMPs ) determine the ligand specificity of the calcitonin receptor like receptor ( CRLR ) ; co expression of RAMP 1 and CRLR results in a CGRP receptor , whereas the association of RAMP 2 or RAMP 3 with CRLR gives an ADM receptor . ^^^ |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| CGRP ( 1 ) receptors are represented by CRLR and RAMP 1 , and specific adrenomedullin receptors by CRLR and RAMP 2 or 3 . ^^^ |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| Thus amylin and CGRP interact specifically with heterodimeric complexes between CTR and RAMP 1 or RAMP 3 , and CGRP and AM interact with complexes between CRLR and RAMP 1 , RAMP 2 or RAMP3 . . ^^^ |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| The selectivity for AM and CGRP agonists and antagonists of human , rat , porcine and bovine CL receptors , co expressed with RAMP 2 and 3 , has been studied in different cell types . ^^^ |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| It is suggested that these may largely be explained by a combination of methodological factors and CGRP responsive receptors generated by CL and RAMP 2 or RAMP 3 and complexes of RAMPs with the calcitonin receptor . . ^^^ |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| The specificity of CRLR was clearly determined by the expression of RAMPs ; RAMP 1 converted CRLR to CGRP receptor , while RAMP 2 and RAMP 3 converted it to AM receptor , but the affinity of CTR for AMY was not increased by the expression of any known RAMPs . ^^^ |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| CRLR functions as either a calcitonin gene related peptide ( CGRP ) receptor or a selective AM receptor depending on which member of a family of receptor activity modifying proteins ( RAMPs ) is expressed : RAMP 1 generates CGRP receptors , while RAMP 2 and RAMP 3 produce AM receptors . ^^^ |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| Calcitonin receptor like receptor ( CRLR ) , a seven domain transmembrane protein functions as CGRP A receptor , in association with receptor activity modifying protein ( RAMP ) 1 , a single domain transmembrane protein , whereas CRLR and RAMP 2 or RAMP 3 constitute a receptor for AM . ^^^ In the present investigation , we examined the mRNA expression of CRLR , RAMP 1 , RAMP 2 , and RAMP 3 in rat uterus ( n = 8 ) by reverse transcriptional analysis and polymerase chain reaction to assess the changes in the expression of CGRP A and AM receptor components during pregnancy and labor and by steroid hormone treatments in adult ovariectomized rats . ^^^ |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| In contrast to CGRP and ADM , which exhibited a preferential stimulation of CRLR when co expressed with RAMP 1 and RAMP 2 or RAMP 3 , respectively , intermedin represents a nonselective agonist for the RAMP coreceptors . ^^^ |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| Coexpression of RAMP 1 and CRLR resulted in functional CGRP 1 receptors ; the complex of RAMP 2 or RAMP 3 and CRLR created functional adrenomedullin receptor . ^^^ Our results demonstrate that CGRP , CRLR , RAMP 1 and RAMP 2 exist in the nucleus accumbens of intact rats , and that they were significantly upregulated in rats with inflammation . ^^^ |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| CGRP receptors are observed in presence of the RAMP1 / calcitonin receptor like receptor ( CRLR ) , but the association of RAMP 2 or RAMP 3 with CRLR generates an adrenomedullin receptor . ^^^ |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| ELT 3 cells expressed CGRP and AM receptor components CRLR , RAMP 1 , RAMP 2 , and RAMP 3 . ^^^ |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| Using reverse transcriptase polymerase chain reaction ( RT PCR ) , the presence of mRNA sequences encoding the calcitonin receptor like receptor , receptor activity modifying protein ( RAMP 1 , RAMP 2 , RAMP 3 ) and receptor component protein were demonstrated in human subcutaneous arteries , indicating the presence of CGRP ( 1 ) like receptor and the necessary component for the receptor activation . ^^^ |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| Cell viability assessed by MTT indicates that , contrary to CGRP , pretreatment of stressed cells with ADM , a RAMP 2 agonist , fails to protect them while treatment with CGRP ( 8 37 ) ( a RAMP 1 and 2 inhibitor ) abolished CGRP protective effect . ^^^ |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| The AM 1 receptor is composed of CLR and RAMP 2 , and the calcitonin gene related peptide ( CGRP ) receptor of CLR and RAMP 1 , as determined by molecular and cell based analysis . ^^^ |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| Only limited pharmacological characterization of the CGRP 1 receptor , a heterodimer of the calcitonin ( CT ) receptor like receptor ( CL ) and receptor activity modifying protein 1 has been performed in cells that do not endogenously express RAMP 2 . ^^^ |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| In particular , the potency of calcitonin gene related peptide ( CGRP ) was most influenced by the C terminal domain present , in which the presence of the RAMP 1 C terminal domain led to increased potency over CTRa alone , whereas chimeras with the RAMP 2 C terminal domain did not induce increased CGRP potency . ^^^ |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| In this study we aimed to assess in vivo , the vasodilator effects of adrenomedullin , proadrenomedullin N terminal 20 peptide ( PAMP ) and amylin in human skin vasculature and compare the responses to the effects mediated by the endogenous neuropeptides calcitonin gene related peptide ( CGRP ) and substance P and to examine the mRNA expression of calcitonin receptor like receptor ( CL R ) and receptor activity modifying proteins , RAMP 1 , RAMP 2 and RAMP 3 in human subcutaneous arteries . ^^^ |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| The receptor activity modifying proteins ( RAMPs ) were shown to be essential for the expression of a functional CRLR and furthermore the RAMPs seemed to modify ligand selectivity of CRLR : coexpression of CRLR and RAMP 1 resulted in a CGRP 1 type of receptor while an adrenomedullin receptor resulted when CRLR and RAMP 2 were coexpressed . ^^^ |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| Using reverse transcriptase polymerase chain reaction the presence of mRNA sequences encoding the CRLR , RAMP 1 and RAMP 2 were demonstrated in porcine left anterior descending ( LAD ) coronary arteries , whereas porcine calcitonin ( CT ) receptor mRNA was not present . ^^^ |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| The receptors have been named AMY ( 1 ( a ) ) , AMY ( 2 ( a ) ) and AMY ( 3 ( a ) ) in accordance with the association of the CT receptor ( CT ( ( a ) ) ) with RAMP 1 , RAMP 2 and RAMP 3 respectively . ^^^ |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| Osteoclast like cells expressed CT receptor ( CTR ) , CT receptor like receptor ( CRLR ) and RAMP 2 , but did not express RAMP 1 or RAMP 3 . ^^^ |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01258 and O60895 |
Pubmed |
SVM Score :0.0 |
| NA |
|