Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.73439895
Here we show that the CGRP binding protein referred to as the calcitonin receptor like receptor is highly concentrated at the avian neuromuscular junction together with its two accessory proteins , receptor activity modifying protein 1 and CGRP receptor component protein , required for ligand specificity and signal transduction . 0.73439895^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.56376131
Previous studies on the calcitonin like receptor ( CLR ) , which requires co expression of the receptor activity modifying protein 1 ( RAMP 1 ) to function as a CGRP receptor , have shown an 18 amino acid N terminus sequence important for binding CGRP . 0.56376131^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
The calcitonin receptor like receptor ( CRLR ) is a seven transmembrane domain ( 7TM ) protein that requires the receptor activity modifying protein 1 ( RAMP 1 ) to be expressed at the cell surface as a functional calcitonin gene related peptide ( CGRP ) receptor . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
Receptor activity modifying protein 1 determines the species selectivity of non peptide CGRP receptor antagonists . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
RESULTS : mRNA transcripts for the cell surface CGRP receptor subunits receptor activity modifying protein 1 ( RAMP 1 ) and calcitonin receptor like receptor ( CRLR ) as well as for the intracellular adapter protein CGRP receptor component protein ( CGRP RCP ) were found in CD 34 ( + ) cells from 4 / 4 donors tested . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
In this study , we cloned mouse cDNA counterparts of the human CGRP receptor composed of calcitonin receptor like receptor ( CRLR ) and receptor activity modifying protein 1 ( RAMP 1 ) and examined the signal transduction mechanism through the CGRP receptor . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
The receptor for calcitonin gene related peptide ( CGRP ) is a heterodimer formed by calcitonin receptor like receptor ( CRLR ) , a type 2 ( family B ) G protein coupled receptor , and receptor activity modifying protein 1 ( RAMP 1 ) , a single membrane pass protein . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
RCP works in conjunction with two other proteins to constitute a functional CGRP receptor : calcitonin receptor like receptor ( CRLR ) and receptor activity modifying protein 1 ( RAMP 1 ) . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
There appear to be two receptors for CGRP : the CGRP A receptor , a well characterized receptor consisting of calcitonin receptor like receptor and receptor activity modifying protein 1 , and the CGRP B receptor . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
A functional calcitonin gene related peptide ( CGRP ) receptor requires dimerization of calcitonin receptor like receptor ( CRLR ) with receptor activity modifying protein 1 ( RAMP 1 ) . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
Furthermore , the antibody could not detect any immunoreactive protein in HEK 293 cells transiently transfected with CRLR and receptor activity modifying protein 1 ( RAMP ( 1 ) ) indicating the possible existence of another CGRP receptor , which does not involve CRLR . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
Expression of components of the CGRP receptor , calcitonin receptor like receptor ( CRLR ) and receptor activity modifying protein 1 ( RAMP 1 ) , was determined by the use of Western blot analysis . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
The present study was aimed to investigate mRNA expression of CGRP A receptor components , calcitonin receptor like receptor ( CRLR ) and receptor activity modifying protein 1 ( RAMP ( 1 ) ) in the rat placenta . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
CGRP receptor belongs to the calcitonin receptor like receptor ( CRLR ) family of G protein coupled receptors and has been shown to require a single transmembrane domain protein called receptor activity modifying protein 1 ( RAMP 1 ) for its functional expression as well as activity . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
The present study was designed to examine the existence of CGRP A receptor components , the calcitonin receptor like receptor ( CRLR ) and receptor activity modifying protein 1 ( RAMP 1 ) , in the human placenta and the vasoactivity of CGRP in the fetoplacental circulation . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
The present study was designed to investigate the expression of CGRP receptor components , calcitonin receptor like receptor ( CRLR ) , and receptor activity modifying protein 1 ( RAMP 1 ) , and the relaxation response to CGRP in fetoplacental vessels from normotensive pregnant women and women with preeclampsia . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
Western blots showed that calcitonin receptor like receptor ( CRLR ) and receptor activity modifying protein 1 ( RAMP 1 ) , which constitute a CGRP receptor , were significantly upregulated in mesenteric arteries in the Ang 2 group compared to the other three groups ( P < 0 . 05 ) . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
BACKGROUND : Receptor activity modifying protein 1 ( RAMP 1 ) is a single transmembrane domain protein required for the functional expression of calcitonin gene related peptide ( CGRP ) receptors . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
These cells express mRNA for CGRP receptor components , calcitonin receptor like receptor , and receptor activity modifying protein 1 . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
Calcitonin receptor like receptor ( CLR ) and receptor activity modifying protein 1 ( RAMP 1 ) comprise a receptor for calcitonin gene related peptide ( CGRP ) and intermedin . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
First , we detected expression of the CGRP receptor subunits , calcitonin receptor like receptor and receptor activity modifying protein 1 in the GT 1 7 cells by reverse transcriptase polymerase chain reaction . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
The calcitonin gene related peptide ( CGRP ) receptor is a heterodimer of a family B G protein coupled receptor , calcitonin receptor like receptor ( CLR ) , and the accessory protein receptor activity modifying protein 1 . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
The receptor for CGRP is an unusual complex of the G protein coupled calcitonin like receptor and an obligate receptor activity modifying protein 1 ( RAMP 1 ) . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
Only limited pharmacological characterization of the CGRP 1 receptor , a heterodimer of the calcitonin ( CT ) receptor like receptor ( CL ) and receptor activity modifying protein 1 has been performed in cells that do not endogenously express RAMP 2 . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
OBJECTIVE : We analysed the effect of aldosterone on calcitonin gene related peptide ( CGRP ) mediated vasodilation in noradrenaline precontracted endothelium denuded mesenteric arteries segments from Wistar Kyoto rats ( WKY ) and spontaneously hypertensive rats ( SHR ) and the effect of aldosterone on calcitonin receptor like receptor ( CL receptor ) and receptor activity modifying protein 1 ( RAMP 1 ) expression in endothelium denuded mesenteric arteries from SHR rats . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
Primary cultures of human osteoblast like cells ( hOB ) express calcitonin receptor like receptor ( CLR ) and receptor activity modifying protein 1 , and , because CGRP stimulates cAMP ( one of the modulators of OPG production in osteoblasts ) , it was investigated whether it affects OPG secretion and expression in hOB . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
The study was performed in the presence or absence of the CGRP 1 ( calcitonin receptor like receptor ( CALCRL ) / receptor activity modifying protein 1 ( RAMP 1 ) ) receptor antagonists BIBN4096BS , CGRP ( 8 37 ) and the adrenomedullin receptor antagonist adrenomedullin ( 22 52 ) . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
CTR has been reported to act as an amylin receptor when heterodimerized with receptor activity modifying protein 1 ( RAMP 1 ) or RAMP 3 . mRNA expression of RAMP 1 and RAMP 3 in mammary tissue decreased during pregnancy and lactation , and amylin mRNA was undetectable , suggesting that up regulated CTR in lactating mammary tissues binds CT rather than amylin . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
RAMP 1 presents the receptor at the cell surface as a mature glycoprotein and a CGRP receptor . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
The receptor activity modifying proteins ( RAMPs ) were shown to be essential for the expression of a functional CRLR and furthermore the RAMPs seemed to modify ligand selectivity of CRLR : coexpression of CRLR and RAMP 1 resulted in a CGRP 1 type of receptor while an adrenomedullin receptor resulted when CRLR and RAMP 2 were coexpressed . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
In Xenopus oocytes , expression of RAMP 1 , which potentiates an endogenous CGRP receptor , led to constitutive activation of co expressed GIRK potassium channels . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
In Xenopus oocytes with an endogenous calcitonin gene related peptide ( CGRP ) receptor , a receptor activity modifying protein ( RAMP 1 ) enhancing CGRP stimulated chloride currents of the cystic fibrosis transmembrane regulator was recently cloned [ McLatchie , L . ^^^ In conclusion , expression of a CGRP receptor in RAEC requires RAMP 1 . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
In conclusion , the recognition of RAMP 1 and 2 as mediators of CRLR expression as a CGRP or ADM receptor has been extended , with evidence that endogenous RAMP 2 is sufficient to reveal an ADM receptor in UMR 106 cells . ^^^ Inhibition of RAMP 2 evoked ADM receptor expression by RAMP 1 and generation of a CGRP receptor is consistent with competitive interactions of the different RAMPs with rCRLR . . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
RAMPs 1 , 2 , and 3 transport CRLR to the plasma membrane with similar efficiencies , however RAMP 1 presents CRLR as a terminally glycosylated , mature glycoprotein and a CGRP receptor , whereas RAMPs 2 and 3 present CRLR as an immature , core glycosylated ADM receptor . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
RAMP 1 transported CRLR is a calcitonin gene related peptide ( CGRP ) receptor . ^^^ Cotransfection of RAMP 1 or RAMP 3 with the human CT receptor lacking the 16 amino acid insert in intracellular domain 1 ( hCTRI 1 ) into COS 7 cells induced specific 125I labeled rat amylin binding . ^^^ The RAMP 1 derived amylin receptor demonstrated the highest affinity for salmon CT ( IC 50 , 3 . 01 + / 1 . 44 10 10 ( 10 ) M ) , a high to moderate affinity for rat amylin ( IC 50 , 7 . 86 + / 4 . 49 10 10 ( 9 ) M ) and human CGRPalpha ( IC 50 , 2 . 09 + / 1 . 63 10 10 ( 8 ) M ) , and a low affinity for human CT ( IC 50 , 4 . 47 + / 0 . 78 10 10 ( 7 ) M ) . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
Here , Drosophila Schneider ( S 2 ) cells stably co expressed rCRLR and RAMP 1 or 2 as functional CGRP or ADM receptors . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
In this study it is shown that rat CRLR cDNA specifies a CGRP receptor when co transfected with RAMP 1 cDNA and an adrenomedullin receptor when co transfected with either RAMP 2 or RAMP 3 cDNA in human embryonic kidney 293 cells . 3 . ^^^ CGRP binding correlates well with RAMP 1 mRNA levels ( R=1 . 0 , P=0 . 007 ) , adrenomedullin binding shows a tendency to vary with RAMP 2 mRNA levels ( R=0 . 85 , P=0 . 14 ) and total binding is correlated with CRLR mRNA levels ( R=0 . 94 , P=0 . 03 ) . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
Cell surface complexes of human RAMP 1 ( hRAMP 1 ) and human calcitonin ( CT ) receptor isotype 2 ( hCTR 2 ) or rat CT receptor like receptor ( rCRLR ) have now been identified through protein cross linking , co immunoprecipitation and confocal microscopy . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
Receptor activity modifying proteins ( RAMPs ) determine the ligand specificity of CRLR : coexpression of CRLR and RAMP 1 results in a CGRP 1 receptor , whereas coexpression of CRLR and RAMP 2 or 3 results in an ADM receptor . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
The initially orphan human calcitonin ( CT ) receptor like receptor ( hCRLR ) interacts with novel accessory receptor activity modifying protein 1 ( RAMP 1 ) to reveal a functional CT gene related peptide ( CGRP ) receptor . ^^^ In contrast , the hCRLR Asn ( 60 , 112 ) to Thr double mutant exhibited defective RAMP 1 dependent N glycosylation , and impaired cell surface expression and CGRP receptor function . ^^^ Unlike Asn ( 60 ) and Asn ( 112 ) , Asn ( 117 ) is normally not N glycosylated , but essential for CGRP binding to the hCRLR RAMP 1 complex . . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
A mature , fully glycosylated , form of CRLR was associated with ( 125 ) 1 CGRP binding , upon co expression of RAMP 1 and CRLR . ^^^ Similarly , ( 125 ) 1 CGRP selectively recognized the mature CRLR species upon co expression with RAMP 1 , indicating that the glycosylation does not determine ligand binding selectivity . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
Calcitonin receptor like receptor ( CRLR ) constitutes either a CGRP receptor when complexed with receptor activity modifying protein 1 ( RAMP 1 ) or an adrenomedullin receptor when complexed with RAMP 2 or RAMP 3 . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
RAMP 1 presents the CRLR at the cell surface as a CGRP / AM receptor . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
Using reverse transcriptase polymerase chain reaction the presence of mRNA sequences encoding the CRLR , RAMP 1 and RAMP 2 were demonstrated in porcine left anterior descending ( LAD ) coronary arteries , whereas porcine calcitonin ( CT ) receptor mRNA was not present . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
As expected CRLR RAMP 1 coexpression conferred sensitivity to CGRP , whilst association of RAMP 2 or RAMP 3 with CRLR conferred high affinity ADM binding . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
Co expression of RAMP 1 and CRLR reconstituted a CGRP receptor that was able to activate the pheromone signaling pathway with pharmacological properties similar to those observed previously in mammalian cells . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
Messenger RNA analysis of the candidate genes for CGRP and adrenomedullin receptors revealed an abundance of both CRLR and RAMP 1 mRNA , suggesting that these genes encode one of the CGRP receptors in this tissue . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
In the present study , we examined the expression of the CGRP receptor activity modifying proteins ( RAMP 1 , RAMP 2 and RAMP 3 ) and receptor component protein ( RCP ) in human brain astrocytes ( AST ) , cerebromicrovascular endothelial ( EC ) and smooth muscle ( SMC ) cells in culture . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
Co expression of CRLR with RAMP 1 or 2 revealed CGRP or AM receptors , respectively . ^^^ The same CTR is a CGRP / amylin or an amylin receptor upon co expression with RAMP 1 or 3 , respectively . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
The calcitonin like receptor ( CL receptor ) requires a single transmembrane domain protein , termed receptor activity modifying protein , RAMP 1 , to function as a CGRP receptor . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
Receptor activity modifying proteins ( RAMPs ) determine the ligand specificity of the calcitonin receptor like receptor ( CRLR ) ; co expression of RAMP 1 and CRLR results in a CGRP receptor , whereas the association of RAMP 2 or RAMP 3 with CRLR gives an ADM receptor . ^^^ The present results suggested after congestive heart failure in adult rats , an up regulation of the CGRP receptor ( by an increase in RAMP 1 that is associated with CRLR ) in atria and ventricles and of ADM receptor ( by increased RAMP 3 expression that is associated with CRLR ) in atria . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
CGRP ( 1 ) receptors are represented by CRLR and RAMP 1 , and specific adrenomedullin receptors by CRLR and RAMP 2 or 3 . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
An amylin / CGRP receptor is revealed when CTR is co expressed with RAMP 1 . ^^^ CRLR , initially classed as an orphan receptor , is a CGRP receptor when co expressed with RAMP 1 . ^^^ CTR RAMP 1 , antagonized preferentially by salmon CT ( 8 32 ) and not by CGRP ( 8 37 ) , and CRLR RAMP 1 , antagonized by CGRP ( 8 37 ) , are two CGRP receptor isotypes . ^^^ Thus amylin and CGRP interact specifically with heterodimeric complexes between CTR and RAMP 1 or RAMP 3 , and CGRP and AM interact with complexes between CRLR and RAMP 1 , RAMP 2 or RAMP3 . . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
Here , we have investigated the functional relevance of the short C terminal intracellular tail QSKRTEGIV and of the single transmembrane domain of human ( h ) RAMP 1 for their interactions with the hCRLR to constitute a CGRP receptor . ^^^ RAMP 1 is transported to the cell surface together with the CRLR or the CT receptor . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
Co expression of the CL receptor and RAMP 1 , formed a CGRP receptor , as in mammals . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
The pCT hCL receptor was a fully functional CGRP receptor when co expressed with RAMP 1 , but the RAMP 2 dependent AM receptor function was impaired . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
The initially orphan calcitonin receptor like receptor ( CRLR ) was identified as a CGRP receptor when coexpressed with RAMP 1 . ^^^ An amylin / CGRP receptor was recognized when a calcitonin receptor ( CTR ) was coexpressed with RAMP 1 . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
RAMP 1 is an accessory protein for another putative CGRP receptor , calcitonin receptor like receptor . ^^^ Antisense oligodeoxyribonucleotides ( ASODNs , 5 mg . kg 1 . day 1 or 5 and 10 mg . kg 1 . day 1 for RDC 1 ) targeting RAMP 1 and RDC 1 mRNAs were chronically infused to the pulmonary circulation of male Sprague Dawley rats during 7 days of normoxia or hypobaric hypoxia ( 380 mmHg ) , and alpha CGRP ASODN was used as a technical control . ^^^ CGRP , RAMP 1 , and RDC 1 ASODNs significantly elevated pulmonary artery pressure ( PPA ) in chronic hypoxic rats compared with hypoxic mismatched ASODN ( MMODN ) and saline vehicle controls . ^^^ CGRP and RAMP 1 ASODNs raised PPA in normoxic rats briefly exposed to 10 % O 2 above MMODN and saline controls . ^^^ These data confirm the protective role of CGRP in the pulmonary circulation and suggest that endogenous RAMP 1 and RDC 1 are essential in regulation of PPA in hypoxia . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
The specificity of CRLR was clearly determined by the expression of RAMPs ; RAMP 1 converted CRLR to CGRP receptor , while RAMP 2 and RAMP 3 converted it to AM receptor , but the affinity of CTR for AMY was not increased by the expression of any known RAMPs . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
CRLR functions as either a calcitonin gene related peptide ( CGRP ) receptor or a selective AM receptor depending on which member of a family of receptor activity modifying proteins ( RAMPs ) is expressed : RAMP 1 generates CGRP receptors , while RAMP 2 and RAMP 3 produce AM receptors . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
In the present investigation , we examined the mRNA expression of CRLR , RAMP 1 , RAMP 2 , and RAMP 3 in rat uterus ( n = 8 ) by reverse transcriptional analysis and polymerase chain reaction to assess the changes in the expression of CGRP A and AM receptor components during pregnancy and labor and by steroid hormone treatments in adult ovariectomized rats . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
We also hypothesized that the expression of CGRP A receptor components , calcitonin receptor like receptor ( CRLR ) , receptor activity modifying protein ( RAMP 1 ) , and CGRP B receptors are greater in pregnant rats . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
In contrast to CGRP and ADM , which exhibited a preferential stimulation of CRLR when co expressed with RAMP 1 and RAMP 2 or RAMP 3 , respectively , intermedin represents a nonselective agonist for the RAMP coreceptors . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
The CGRP A receptor consists of calcitonin receptor like receptor ( CRLR ) and receptor activity modifying protein 1 ( RAMP 1 ) ; however , the CGRP B receptor needs to be further characterized . ^^^ Messenger RNA levels for CRLR and RAMP 1 were assessed by reverse transcription polymerase chain reaction , and CGRP B receptor proteins levels were determined by Western blot analysis . ^^^ Both mRNA for CGRP A ( CRLR and RAMP 1 ) and the protein for CGRP B receptors in mesenteric arteries were increased with pregnancy compared to nonpregnant , diestrous animals . ^^^ In adult ovariectomized rats , P 4 upregulated CRLR and RAMP 1 mRNA levels as well as [ 125I ] CGRP binding sites . ^^^ Together with earlier findings , these data suggest that increases in the expression of CGRP A ( CRLR and RAMP 1 ) and CGRP B receptors in mesenteric arteries may be important in reducing vascular resistance and in vascular adaptations that occur during pregnancy ; in addition , P 4 may be involved in this process . . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
In addition , AM has appreciable affinity for the CGRP 1 receptor , composed of CL and RAMP 1 . ^^^ The CGRP selectivity of RAMP 1 and RAMP 3 may be conferred by a putative disulfide bond from the N terminus to the middle of the extracellular domain of these molecules . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
Coexpression of RAMP 1 and CRLR resulted in functional CGRP 1 receptors ; the complex of RAMP 2 or RAMP 3 and CRLR created functional adrenomedullin receptor . ^^^ In the present study , we used real time quantitative RT PCR to detect and quantitate the relative expression of CGRP , CRLR , RAMP 1 3 and RDC 1 in the nucleus accumbens of intact rats and rats with inflammation . ^^^ Our results demonstrate that CGRP , CRLR , RAMP 1 and RAMP 2 exist in the nucleus accumbens of intact rats , and that they were significantly upregulated in rats with inflammation . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
In conclusion , mutation of D 69 to N , E or A in the CLR did not affect its expression at the cell surface , but impaired or abolished the CGRP and AM receptor function in the presence of RAMP 1 and 2 , respectively . . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
ELT 3 cells expressed CGRP and AM receptor components CRLR , RAMP 1 , RAMP 2 , and RAMP 3 . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
Using reverse transcriptase polymerase chain reaction ( RT PCR ) , the presence of mRNA sequences encoding the calcitonin receptor like receptor , receptor activity modifying protein ( RAMP 1 , RAMP 2 , RAMP 3 ) and receptor component protein were demonstrated in human subcutaneous arteries , indicating the presence of CGRP ( 1 ) like receptor and the necessary component for the receptor activation . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
BIBN4096BS , a CGRP antagonist , targets RAMP 1 and this gives the compound very high selectivity for the human CGRP ( 1 ) receptor . . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
The receptors have been named AMY ( 1 ( a ) ) , AMY ( 2 ( a ) ) and AMY ( 3 ( a ) ) in accordance with the association of the CT receptor ( CT ( ( a ) ) ) with RAMP 1 , RAMP 2 and RAMP 3 respectively . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
RAMP 1 enables CRL to form the CGRP ( 8 37 ) sensitive CGRP ( 1 ) receptor , which can also be activated by higher concentrations of AM . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
The binding of CGRP and its ability to stimulate cAMP production were investigated in mutant and wild type receptors after transient transfection into COS 7 cells with RAMP 1 . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
Osteoclast like cells expressed CT receptor ( CTR ) , CT receptor like receptor ( CRLR ) and RAMP 2 , but did not express RAMP 1 or RAMP 3 . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
RAMP 1 expression was upregulated by CGRP while RAMP 3 mRNA level was decreased . ^^^ Cell viability assessed by MTT indicates that , contrary to CGRP , pretreatment of stressed cells with ADM , a RAMP 2 agonist , fails to protect them while treatment with CGRP ( 8 37 ) ( a RAMP 1 and 2 inhibitor ) abolished CGRP protective effect . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
The concordance of IMD and RAMP 1 up regulation indicates a CGRP type receptor action ; considering also a lack of response to BP reduction , IMD may , like CGRP , have an anti ischemic function . . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
The AM 1 receptor is composed of CLR and RAMP 2 , and the calcitonin gene related peptide ( CGRP ) receptor of CLR and RAMP 1 , as determined by molecular and cell based analysis . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
CLR and CTR are members of the class 2 ( family B ) G protein coupled receptors ( GPCR ) and bind calcitonin gene related peptide ( CGRP ) with similar affinities when coexpressed with RAMP 1 . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
In particular , the potency of calcitonin gene related peptide ( CGRP ) was most influenced by the C terminal domain present , in which the presence of the RAMP 1 C terminal domain led to increased potency over CTRa alone , whereas chimeras with the RAMP 2 C terminal domain did not induce increased CGRP potency . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
CGRP receptor markers ( CRLR and RAMP 1 ) were expressed in invading macrophages . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
In this study we aimed to assess in vivo , the vasodilator effects of adrenomedullin , proadrenomedullin N terminal 20 peptide ( PAMP ) and amylin in human skin vasculature and compare the responses to the effects mediated by the endogenous neuropeptides calcitonin gene related peptide ( CGRP ) and substance P and to examine the mRNA expression of calcitonin receptor like receptor ( CL R ) and receptor activity modifying proteins , RAMP 1 , RAMP 2 and RAMP 3 in human subcutaneous arteries . ^^^
Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
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Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
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Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
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Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
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Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
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Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
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Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
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Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
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Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
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Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
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Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
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Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
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Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
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Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
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Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
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Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
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Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
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Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
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Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
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Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
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Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
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Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
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Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
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Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
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Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
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Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
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Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
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Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
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Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
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Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
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Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
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Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
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Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
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Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
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Interacting proteins: O60894 and P01258	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.73439895
Here we show that the CGRP binding protein referred to as the calcitonin receptor like receptor is highly concentrated at the avian neuromuscular junction together with its two accessory proteins , receptor activity modifying protein 1 and CGRP receptor component protein , required for ligand specificity and signal transduction . 0.73439895^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.56376131
Previous studies on the calcitonin like receptor ( CLR ) , which requires co expression of the receptor activity modifying protein 1 ( RAMP 1 ) to function as a CGRP receptor , have shown an 18 amino acid N terminus sequence important for binding CGRP . 0.56376131^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
RAMP 1 presents the receptor at the cell surface as a mature glycoprotein and a CGRP receptor . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
In Xenopus oocytes , expression of RAMP 1 , which potentiates an endogenous CGRP receptor , led to constitutive activation of co expressed GIRK potassium channels . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
In Xenopus oocytes with an endogenous calcitonin gene related peptide ( CGRP ) receptor , a receptor activity modifying protein ( RAMP 1 ) enhancing CGRP stimulated chloride currents of the cystic fibrosis transmembrane regulator was recently cloned [ McLatchie , L . ^^^ In conclusion , expression of a CGRP receptor in RAEC requires RAMP 1 . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
In conclusion , the recognition of RAMP 1 and 2 as mediators of CRLR expression as a CGRP or ADM receptor has been extended , with evidence that endogenous RAMP 2 is sufficient to reveal an ADM receptor in UMR 106 cells . ^^^ Inhibition of RAMP 2 evoked ADM receptor expression by RAMP 1 and generation of a CGRP receptor is consistent with competitive interactions of the different RAMPs with rCRLR . . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
RAMPs 1 , 2 , and 3 transport CRLR to the plasma membrane with similar efficiencies , however RAMP 1 presents CRLR as a terminally glycosylated , mature glycoprotein and a CGRP receptor , whereas RAMPs 2 and 3 present CRLR as an immature , core glycosylated ADM receptor . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
RAMP 1 transported CRLR is a calcitonin gene related peptide ( CGRP ) receptor . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
Here , Drosophila Schneider ( S 2 ) cells stably co expressed rCRLR and RAMP 1 or 2 as functional CGRP or ADM receptors . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
In this study it is shown that rat CRLR cDNA specifies a CGRP receptor when co transfected with RAMP 1 cDNA and an adrenomedullin receptor when co transfected with either RAMP 2 or RAMP 3 cDNA in human embryonic kidney 293 cells . 3 . ^^^ CGRP binding correlates well with RAMP 1 mRNA levels ( R=1 . 0 , P=0 . 007 ) , adrenomedullin binding shows a tendency to vary with RAMP 2 mRNA levels ( R=0 . 85 , P=0 . 14 ) and total binding is correlated with CRLR mRNA levels ( R=0 . 94 , P=0 . 03 ) . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
Receptor activity modifying proteins ( RAMPs ) determine the ligand specificity of CRLR : coexpression of CRLR and RAMP 1 results in a CGRP 1 receptor , whereas coexpression of CRLR and RAMP 2 or 3 results in an ADM receptor . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
The initially orphan human calcitonin ( CT ) receptor like receptor ( hCRLR ) interacts with novel accessory receptor activity modifying protein 1 ( RAMP 1 ) to reveal a functional CT gene related peptide ( CGRP ) receptor . ^^^ In contrast , the hCRLR Asn ( 60 , 112 ) to Thr double mutant exhibited defective RAMP 1 dependent N glycosylation , and impaired cell surface expression and CGRP receptor function . ^^^ Unlike Asn ( 60 ) and Asn ( 112 ) , Asn ( 117 ) is normally not N glycosylated , but essential for CGRP binding to the hCRLR RAMP 1 complex . . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
A mature , fully glycosylated , form of CRLR was associated with ( 125 ) 1 CGRP binding , upon co expression of RAMP 1 and CRLR . ^^^ Similarly , ( 125 ) 1 CGRP selectively recognized the mature CRLR species upon co expression with RAMP 1 , indicating that the glycosylation does not determine ligand binding selectivity . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
Calcitonin receptor like receptor ( CRLR ) constitutes either a CGRP receptor when complexed with receptor activity modifying protein 1 ( RAMP 1 ) or an adrenomedullin receptor when complexed with RAMP 2 or RAMP 3 . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
RAMP 1 presents the CRLR at the cell surface as a CGRP / AM receptor . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
The calcitonin receptor like receptor ( CRLR ) is a seven transmembrane domain ( 7TM ) protein that requires the receptor activity modifying protein 1 ( RAMP 1 ) to be expressed at the cell surface as a functional calcitonin gene related peptide ( CGRP ) receptor . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
As expected CRLR RAMP 1 coexpression conferred sensitivity to CGRP , whilst association of RAMP 2 or RAMP 3 with CRLR conferred high affinity ADM binding . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
Co expression of RAMP 1 and CRLR reconstituted a CGRP receptor that was able to activate the pheromone signaling pathway with pharmacological properties similar to those observed previously in mammalian cells . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
Messenger RNA analysis of the candidate genes for CGRP and adrenomedullin receptors revealed an abundance of both CRLR and RAMP 1 mRNA , suggesting that these genes encode one of the CGRP receptors in this tissue . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
In the present study , we examined the expression of the CGRP receptor activity modifying proteins ( RAMP 1 , RAMP 2 and RAMP 3 ) and receptor component protein ( RCP ) in human brain astrocytes ( AST ) , cerebromicrovascular endothelial ( EC ) and smooth muscle ( SMC ) cells in culture . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
These observations suggest that important determinants of small molecule antagonist affinity for the CGRP receptor reside within the extracellular region of RAMP 1 and provide evidence that this receptor accessory protein may participate in antagonist binding . . ^^^ Receptor activity modifying protein 1 determines the species selectivity of non peptide CGRP receptor antagonists . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
Co expression of CRLR with RAMP 1 or 2 revealed CGRP or AM receptors , respectively . ^^^ The same CTR is a CGRP / amylin or an amylin receptor upon co expression with RAMP 1 or 3 , respectively . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
RESULTS : mRNA transcripts for the cell surface CGRP receptor subunits receptor activity modifying protein 1 ( RAMP 1 ) and calcitonin receptor like receptor ( CRLR ) as well as for the intracellular adapter protein CGRP receptor component protein ( CGRP RCP ) were found in CD 34 ( + ) cells from 4 / 4 donors tested . ^^^ CGRP RCP mRNA was expressed in peripheral blood granulocytes of 8 / 15 donors , whereas RAMP 1 and CRLR were not detectable . ^^^ Both RAMP 1 and CRLR mRNA expression and CGRP receptor signaling capacity were lost during in vitro granulocytic differentiation of CD 34 ( + ) cells . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
The calcitonin like receptor ( CL receptor ) requires a single transmembrane domain protein , termed receptor activity modifying protein , RAMP 1 , to function as a CGRP receptor . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
The pCT hCL receptor was a fully functional CGRP receptor when co expressed with RAMP 1 , but the RAMP 2 dependent AM receptor function was impaired . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
There appear to be two receptors for CGRP : the CGRP A receptor , a well characterized receptor consisting of calcitonin receptor like receptor and receptor activity modifying protein 1 , and the CGRP B receptor . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
Receptor activity modifying proteins ( RAMPs ) determine the ligand specificity of the calcitonin receptor like receptor ( CRLR ) ; co expression of RAMP 1 and CRLR results in a CGRP receptor , whereas the association of RAMP 2 or RAMP 3 with CRLR gives an ADM receptor . ^^^ The present results suggested after congestive heart failure in adult rats , an up regulation of the CGRP receptor ( by an increase in RAMP 1 that is associated with CRLR ) in atria and ventricles and of ADM receptor ( by increased RAMP 3 expression that is associated with CRLR ) in atria . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
In this study , we cloned mouse cDNA counterparts of the human CGRP receptor composed of calcitonin receptor like receptor ( CRLR ) and receptor activity modifying protein 1 ( RAMP 1 ) and examined the signal transduction mechanism through the CGRP receptor . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
CGRP ( 1 ) receptors are represented by CRLR and RAMP 1 , and specific adrenomedullin receptors by CRLR and RAMP 2 or 3 . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
The receptor for calcitonin gene related peptide ( CGRP ) is a heterodimer formed by calcitonin receptor like receptor ( CRLR ) , a type 2 ( family B ) G protein coupled receptor , and receptor activity modifying protein 1 ( RAMP 1 ) , a single membrane pass protein . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
An amylin / CGRP receptor is revealed when CTR is co expressed with RAMP 1 . ^^^ CRLR , initially classed as an orphan receptor , is a CGRP receptor when co expressed with RAMP 1 . ^^^ CTR RAMP 1 , antagonized preferentially by salmon CT ( 8 32 ) and not by CGRP ( 8 37 ) , and CRLR RAMP 1 , antagonized by CGRP ( 8 37 ) , are two CGRP receptor isotypes . ^^^ Thus amylin and CGRP interact specifically with heterodimeric complexes between CTR and RAMP 1 or RAMP 3 , and CGRP and AM interact with complexes between CRLR and RAMP 1 , RAMP 2 or RAMP3 . . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
RCP works in conjunction with two other proteins to constitute a functional CGRP receptor : calcitonin receptor like receptor ( CRLR ) and receptor activity modifying protein 1 ( RAMP 1 ) . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
Here , we have investigated the functional relevance of the short C terminal intracellular tail QSKRTEGIV and of the single transmembrane domain of human ( h ) RAMP 1 for their interactions with the hCRLR to constitute a CGRP receptor . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
Co expression of the CL receptor and RAMP 1 , formed a CGRP receptor , as in mammals . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
The initially orphan calcitonin receptor like receptor ( CRLR ) was identified as a CGRP receptor when coexpressed with RAMP 1 . ^^^ An amylin / CGRP receptor was recognized when a calcitonin receptor ( CTR ) was coexpressed with RAMP 1 . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
A functional calcitonin gene related peptide ( CGRP ) receptor requires dimerization of calcitonin receptor like receptor ( CRLR ) with receptor activity modifying protein 1 ( RAMP 1 ) . ^^^ These RAMP 1 mutants were examined for their ability to associate with CRLR to effect CGRP stimulated cAMP accumulation , CGRP binding , CRLR trafficking , and cell surface expression . ^^^ All RAMP 1 mutants were able to associate with CRLR with full efficacy for CGRP stimulated cAMP accumulation . ^^^ However , the RAMP 1 / platelet derived growth factor receptor chimera demonstrated a 10 fold decrease in potency for CGRP signaling and binding , and the RAMP 1 ECD mutant had a 4000 fold decrease in potency . ^^^ The presence of a TM domain and the specific sequence of the RAMP 1 TM domain contribute to CGRP affinity and potency . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
Furthermore , the antibody could not detect any immunoreactive protein in HEK 293 cells transiently transfected with CRLR and receptor activity modifying protein 1 ( RAMP ( 1 ) ) indicating the possible existence of another CGRP receptor , which does not involve CRLR . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
RAMP 1 is an accessory protein for another putative CGRP receptor , calcitonin receptor like receptor . ^^^ Antisense oligodeoxyribonucleotides ( ASODNs , 5 mg . kg 1 . day 1 or 5 and 10 mg . kg 1 . day 1 for RDC 1 ) targeting RAMP 1 and RDC 1 mRNAs were chronically infused to the pulmonary circulation of male Sprague Dawley rats during 7 days of normoxia or hypobaric hypoxia ( 380 mmHg ) , and alpha CGRP ASODN was used as a technical control . ^^^ CGRP , RAMP 1 , and RDC 1 ASODNs significantly elevated pulmonary artery pressure ( PPA ) in chronic hypoxic rats compared with hypoxic mismatched ASODN ( MMODN ) and saline vehicle controls . ^^^ CGRP and RAMP 1 ASODNs raised PPA in normoxic rats briefly exposed to 10 % O 2 above MMODN and saline controls . ^^^ These data confirm the protective role of CGRP in the pulmonary circulation and suggest that endogenous RAMP 1 and RDC 1 are essential in regulation of PPA in hypoxia . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
The specificity of CRLR was clearly determined by the expression of RAMPs ; RAMP 1 converted CRLR to CGRP receptor , while RAMP 2 and RAMP 3 converted it to AM receptor , but the affinity of CTR for AMY was not increased by the expression of any known RAMPs . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
Expression of components of the CGRP receptor , calcitonin receptor like receptor ( CRLR ) and receptor activity modifying protein 1 ( RAMP 1 ) , was determined by the use of Western blot analysis . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
CRLR functions as either a calcitonin gene related peptide ( CGRP ) receptor or a selective AM receptor depending on which member of a family of receptor activity modifying proteins ( RAMPs ) is expressed : RAMP 1 generates CGRP receptors , while RAMP 2 and RAMP 3 produce AM receptors . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
In the present investigation , we examined the mRNA expression of CRLR , RAMP 1 , RAMP 2 , and RAMP 3 in rat uterus ( n = 8 ) by reverse transcriptional analysis and polymerase chain reaction to assess the changes in the expression of CGRP A and AM receptor components during pregnancy and labor and by steroid hormone treatments in adult ovariectomized rats . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
The present study was aimed to investigate mRNA expression of CGRP A receptor components , calcitonin receptor like receptor ( CRLR ) and receptor activity modifying protein 1 ( RAMP ( 1 ) ) in the rat placenta . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
We also hypothesized that the expression of CGRP A receptor components , calcitonin receptor like receptor ( CRLR ) , receptor activity modifying protein ( RAMP 1 ) , and CGRP B receptors are greater in pregnant rats . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
CGRP receptor belongs to the calcitonin receptor like receptor ( CRLR ) family of G protein coupled receptors and has been shown to require a single transmembrane domain protein called receptor activity modifying protein 1 ( RAMP 1 ) for its functional expression as well as activity . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
In contrast to CGRP and ADM , which exhibited a preferential stimulation of CRLR when co expressed with RAMP 1 and RAMP 2 or RAMP 3 , respectively , intermedin represents a nonselective agonist for the RAMP coreceptors . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
The CGRP A receptor consists of calcitonin receptor like receptor ( CRLR ) and receptor activity modifying protein 1 ( RAMP 1 ) ; however , the CGRP B receptor needs to be further characterized . ^^^ Messenger RNA levels for CRLR and RAMP 1 were assessed by reverse transcription polymerase chain reaction , and CGRP B receptor proteins levels were determined by Western blot analysis . ^^^ Both mRNA for CGRP A ( CRLR and RAMP 1 ) and the protein for CGRP B receptors in mesenteric arteries were increased with pregnancy compared to nonpregnant , diestrous animals . ^^^ In adult ovariectomized rats , P 4 upregulated CRLR and RAMP 1 mRNA levels as well as [ 125I ] CGRP binding sites . ^^^ Together with earlier findings , these data suggest that increases in the expression of CGRP A ( CRLR and RAMP 1 ) and CGRP B receptors in mesenteric arteries may be important in reducing vascular resistance and in vascular adaptations that occur during pregnancy ; in addition , P 4 may be involved in this process . . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
The present study was designed to examine the existence of CGRP A receptor components , the calcitonin receptor like receptor ( CRLR ) and receptor activity modifying protein 1 ( RAMP 1 ) , in the human placenta and the vasoactivity of CGRP in the fetoplacental circulation . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
In addition , AM has appreciable affinity for the CGRP 1 receptor , composed of CL and RAMP 1 . ^^^ The CGRP selectivity of RAMP 1 and RAMP 3 may be conferred by a putative disulfide bond from the N terminus to the middle of the extracellular domain of these molecules . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
Coexpression of RAMP 1 and CRLR resulted in functional CGRP 1 receptors ; the complex of RAMP 2 or RAMP 3 and CRLR created functional adrenomedullin receptor . ^^^ In the present study , we used real time quantitative RT PCR to detect and quantitate the relative expression of CGRP , CRLR , RAMP 1 3 and RDC 1 in the nucleus accumbens of intact rats and rats with inflammation . ^^^ Our results demonstrate that CGRP , CRLR , RAMP 1 and RAMP 2 exist in the nucleus accumbens of intact rats , and that they were significantly upregulated in rats with inflammation . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
In conclusion , mutation of D 69 to N , E or A in the CLR did not affect its expression at the cell surface , but impaired or abolished the CGRP and AM receptor function in the presence of RAMP 1 and 2 , respectively . . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
ELT 3 cells expressed CGRP and AM receptor components CRLR , RAMP 1 , RAMP 2 , and RAMP 3 . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
Using reverse transcriptase polymerase chain reaction ( RT PCR ) , the presence of mRNA sequences encoding the calcitonin receptor like receptor , receptor activity modifying protein ( RAMP 1 , RAMP 2 , RAMP 3 ) and receptor component protein were demonstrated in human subcutaneous arteries , indicating the presence of CGRP ( 1 ) like receptor and the necessary component for the receptor activation . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
BIBN4096BS , a CGRP antagonist , targets RAMP 1 and this gives the compound very high selectivity for the human CGRP ( 1 ) receptor . . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
RAMP 1 enables CRL to form the CGRP ( 8 37 ) sensitive CGRP ( 1 ) receptor , which can also be activated by higher concentrations of AM . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
The binding of CGRP and its ability to stimulate cAMP production were investigated in mutant and wild type receptors after transient transfection into COS 7 cells with RAMP 1 . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
The present study was designed to investigate the expression of CGRP receptor components , calcitonin receptor like receptor ( CRLR ) , and receptor activity modifying protein 1 ( RAMP 1 ) , and the relaxation response to CGRP in fetoplacental vessels from normotensive pregnant women and women with preeclampsia . ^^^ In contrast , both vascular tissues and trophoblasts showed decreased expressions for CRLR and RAMP 1 proteins and declined CGRP binding sites in preeclamptic placentas ; and 3 ) CGRP produced a dose dependent relaxation of serotonin induced contraction of umbilical and chorionic arteries from normal pregnancies , but the response to CGRP was significantly attenuated in the vessels from preeclampsia . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
Western blots showed that calcitonin receptor like receptor ( CRLR ) and receptor activity modifying protein 1 ( RAMP 1 ) , which constitute a CGRP receptor , were significantly upregulated in mesenteric arteries in the Ang 2 group compared to the other three groups ( P < 0 . 05 ) . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
BACKGROUND : Receptor activity modifying protein 1 ( RAMP 1 ) is a single transmembrane domain protein required for the functional expression of calcitonin gene related peptide ( CGRP ) receptors . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
These cells express mRNA for CGRP receptor components , calcitonin receptor like receptor , and receptor activity modifying protein 1 . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
Calcitonin receptor like receptor ( CLR ) and receptor activity modifying protein 1 ( RAMP 1 ) comprise a receptor for calcitonin gene related peptide ( CGRP ) and intermedin . ^^^ Although CGRP is widely expressed in the nervous system , less is known about the localization of CLR and RAMP 1 . ^^^ Fluorescent CGRP specifically bound to the surface of these cells and CGRP , CLR , and RAMP 1 internalized into the same endosomes . ^^^ Thus , CLR and RAMP 1 may mediate the effects of CGRP and intermedin in the nervous system . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
First , we detected expression of the CGRP receptor subunits , calcitonin receptor like receptor and receptor activity modifying protein 1 in the GT 1 7 cells by reverse transcriptase polymerase chain reaction . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
RAMP 1 expression was upregulated by CGRP while RAMP 3 mRNA level was decreased . ^^^ Cell viability assessed by MTT indicates that , contrary to CGRP , pretreatment of stressed cells with ADM , a RAMP 2 agonist , fails to protect them while treatment with CGRP ( 8 37 ) ( a RAMP 1 and 2 inhibitor ) abolished CGRP protective effect . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
The calcitonin gene related peptide ( CGRP ) receptor is a heterodimer of a family B G protein coupled receptor , calcitonin receptor like receptor ( CLR ) , and the accessory protein receptor activity modifying protein 1 . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
The concordance of IMD and RAMP 1 up regulation indicates a CGRP type receptor action ; considering also a lack of response to BP reduction , IMD may , like CGRP , have an anti ischemic function . . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
The receptor for CGRP is an unusual complex of the G protein coupled calcitonin like receptor and an obligate receptor activity modifying protein 1 ( RAMP 1 ) . ^^^ Although cultured rat aorta smooth muscle cells express calcitonin like receptor and RAMP 1 , we found that CGRP is not a potent activator of the receptor . ^^^ After overexpression of RAMP 1 by adenoviral gene transfer , there was a striking increase in CGRP induced production of cAMP , with a 75 fold decrease in the EC ( 50 ) and a 1 . 5 fold increase in the maximal response . ^^^ First , RAMP 1 gene transfer caused a CGRP dependent decrease in cell proliferation . ^^^ Second , RAMP 1 and CGRP treatment led to a 3 fold greater free radical induced reduction in cell number . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
The AM 1 receptor is composed of CLR and RAMP 2 , and the calcitonin gene related peptide ( CGRP ) receptor of CLR and RAMP 1 , as determined by molecular and cell based analysis . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
Only limited pharmacological characterization of the CGRP 1 receptor , a heterodimer of the calcitonin ( CT ) receptor like receptor ( CL ) and receptor activity modifying protein 1 has been performed in cells that do not endogenously express RAMP 2 . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
OBJECTIVE : We analysed the effect of aldosterone on calcitonin gene related peptide ( CGRP ) mediated vasodilation in noradrenaline precontracted endothelium denuded mesenteric arteries segments from Wistar Kyoto rats ( WKY ) and spontaneously hypertensive rats ( SHR ) and the effect of aldosterone on calcitonin receptor like receptor ( CL receptor ) and receptor activity modifying protein 1 ( RAMP 1 ) expression in endothelium denuded mesenteric arteries from SHR rats . ^^^ CONCLUSIONS : Aldosterone , through glucocorticoid receptor activation , increases the vasodilatory effect of CGRP in SHR mesenteric arteries , which seems to be mediated by increased RAMP 1 expression . . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
CLR and CTR are members of the class 2 ( family B ) G protein coupled receptors ( GPCR ) and bind calcitonin gene related peptide ( CGRP ) with similar affinities when coexpressed with RAMP 1 . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
In particular , the potency of calcitonin gene related peptide ( CGRP ) was most influenced by the C terminal domain present , in which the presence of the RAMP 1 C terminal domain led to increased potency over CTRa alone , whereas chimeras with the RAMP 2 C terminal domain did not induce increased CGRP potency . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
Primary cultures of human osteoblast like cells ( hOB ) express calcitonin receptor like receptor ( CLR ) and receptor activity modifying protein 1 , and , because CGRP stimulates cAMP ( one of the modulators of OPG production in osteoblasts ) , it was investigated whether it affects OPG secretion and expression in hOB . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
The study was performed in the presence or absence of the CGRP 1 ( calcitonin receptor like receptor ( CALCRL ) / receptor activity modifying protein 1 ( RAMP 1 ) ) receptor antagonists BIBN4096BS , CGRP ( 8 37 ) and the adrenomedullin receptor antagonist adrenomedullin ( 22 52 ) . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
CGRP receptor markers ( CRLR and RAMP 1 ) were expressed in invading macrophages . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
In this study we aimed to assess in vivo , the vasodilator effects of adrenomedullin , proadrenomedullin N terminal 20 peptide ( PAMP ) and amylin in human skin vasculature and compare the responses to the effects mediated by the endogenous neuropeptides calcitonin gene related peptide ( CGRP ) and substance P and to examine the mRNA expression of calcitonin receptor like receptor ( CL R ) and receptor activity modifying proteins , RAMP 1 , RAMP 2 and RAMP 3 in human subcutaneous arteries . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
The receptor activity modifying proteins ( RAMPs ) were shown to be essential for the expression of a functional CRLR and furthermore the RAMPs seemed to modify ligand selectivity of CRLR : coexpression of CRLR and RAMP 1 resulted in a CGRP 1 type of receptor while an adrenomedullin receptor resulted when CRLR and RAMP 2 were coexpressed . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
Cell surface complexes of human RAMP 1 ( hRAMP 1 ) and human calcitonin ( CT ) receptor isotype 2 ( hCTR 2 ) or rat CT receptor like receptor ( rCRLR ) have now been identified through protein cross linking , co immunoprecipitation and confocal microscopy . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
Using reverse transcriptase polymerase chain reaction the presence of mRNA sequences encoding the CRLR , RAMP 1 and RAMP 2 were demonstrated in porcine left anterior descending ( LAD ) coronary arteries , whereas porcine calcitonin ( CT ) receptor mRNA was not present . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
The receptors have been named AMY ( 1 ( a ) ) , AMY ( 2 ( a ) ) and AMY ( 3 ( a ) ) in accordance with the association of the CT receptor ( CT ( ( a ) ) ) with RAMP 1 , RAMP 2 and RAMP 3 respectively . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
Osteoclast like cells expressed CT receptor ( CTR ) , CT receptor like receptor ( CRLR ) and RAMP 2 , but did not express RAMP 1 or RAMP 3 . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
CTR has been reported to act as an amylin receptor when heterodimerized with receptor activity modifying protein 1 ( RAMP 1 ) or RAMP 3 . mRNA expression of RAMP 1 and RAMP 3 in mammary tissue decreased during pregnancy and lactation , and amylin mRNA was undetectable , suggesting that up regulated CTR in lactating mammary tissues binds CT rather than amylin . ^^^
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01258 and O60894	Pubmed	SVM Score :0.0
NA