| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| Moreover , perinatal hypothyroid animals demonstrated a dramatic increase in the ratio of sialylated to sulfated species within oligosaccharides of the same negative charge ( 2 . 9 to 7 . 4 fold increase for TSH alpha ; 15 . 1 to 25 . 5 fold increase for TSH beta ) . ^^^ |
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| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| Chromogranin A was expressed frequently in adenomas in which at least one of the FSH alpha , FSH beta , LH beta and TSH beta subunits was positive . ^^^ |
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| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| The distribution pattern of these forms appeared totally distinct from that of individual TSH alpha ( pI 8 . 8 , 8 . 4 , 8 . 2 , 7 . 6 , 7 . 4 , 6 . 8 , 6 . 6 , 5 . 8 , and 5 . 4 ) and TSH beta ( pI 8 . 7 , 8 . 1 , 7 . 2 , 6 . 8 , 6 . 2 , and 5 . 8 ) subunits . ^^^ |
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| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| Each of the three secreted subunits ( TSH alpha , TSH beta , and free alpha ) incorporated both sulfate and sialic acid . ^^^ In secreted TSH dimer , TSH beta incorporated 1 . 3 times more [ 35S ] sulfate ( P less than 0 . 05 ) and 2 . 5 times more [ 3H ] N acetylmannosamine ( P less than 0 . 02 ) per carbohydrate chain than did TSH alpha . ^^^ TSH alpha and TSH beta were affected equally . ^^^ |
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| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| The immunicytological analysis of PAE fixed semi thin sections of pituitary cells ( of human fetuses aged 6 to 26 weeks ) using immunesera directed against the human total glycoproteic hormones , HCG , LH , FSH and TSH or made specific for the LH , FSH , and TSH beta subunits shows : the presence up to the 12th week of only the subunit of the glycoproteic hormones revealed indiffrently by our anti total hormones immunesera ; the apparition of the betaTSH subunits at the 13th week , characterizing the differentiation of the thyrotrophs ; the apparition of betaLH and betaFSH subunits on the one and same gonadotropic cell population at the 15th week . . ^^^ |
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| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| We found that CDR 1 of 4G11K and CDR 2 of D 2 mu show sequence similarity to regions of TSH alpha and TSH beta that had been previously implicated in the interaction of the hormone with its receptor . ^^^ |
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| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| By dual metabolic labeling with [ 35S ] sulfate and [ 3H ] mannose , we have characterized oligosaccharides from secreted TSH alpha , TSH beta , and free alpha subunits released from the apoprotein by enzymatic deglycosylation . ^^^ Analysis of [ 3H ] mannose labeled oligosaccharides on Concanavalin A agarose showed 85 % binding for those from TSH alpha , 70 % for free alpha , and 50 % for those from TSH beta . ^^^ These data demonstrate that oligosaccharides from secreted TSH beta were more sialylated and sulfated , consistent with a more complex branching pattern , than those from TSH alpha . ^^^ Oligosaccharides from free alpha subunit were more sialylated than those from TSH alpha , and the net negative charge was intermediate between those of TSH alpha and TSH beta . ^^^ |
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| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| Antisera were used against human PRL , human GH , ACTH 1 24 , human ACTH 17 39 , alpha melanocyte stimulating hormone ( alpha MSH ) , human beta endorphin , alpha subunit of hCG ( hCG alpha ) , and beta subunits of human LH ( LH beta ) , human FSH ( FSH beta ) and human TSH ( TSH beta ) . ^^^ Exclusive immunostaining was present in 6 cases : 4 with hCG alpha , 1 with ACTH 1 24 and 1 with TSH beta . ^^^ |
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| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| By covalently crosslinking TSH to its receptor and characterizing the product with antisera specific for either the alpha or beta subunit of TSH , we have shown that the TSH beta subunit but not the TSH alpha subunit crosslinks to the TSH receptor . . ^^^ |
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| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| Dispersed mouse thyrotropic tumor cells were labeled by pulse chase methods with [ 35S ] methionine and various 3H labeled carbohydrates ; cell lysates and media were precipitated with antisera to TSH alpha and TSH beta , and the products were analyzed by sodium dodecyl sulfate gradient gel electrophoresis without or with preexposure to Endoglycosidase ( Endo ) H . ^^^ |
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| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| Immunohistochemical analysis of serial 2 micron sections of the tumor showed that : 1 ) TSH alpha immunoreactive cells were the most numerous , 2 ) TSH beta positive cells were always positive for TSH alpha , 3 ) PRL immunoreactivity was found either uniquely in some cells and colocalized with TSH alpha immunoreactivity in other cells . ^^^ |
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| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| Antisera to human pituitary hormones cross reacted either with cells known to produce corresponding hormones ( or hormone subunits ) in the platyfish ( e . g . , ACTH , prolactin , TSH beta , LH alpha , FSH alpha , TSH alpha ) or with no pituitary cells at all ( e . g . , HL beta , FSH beta ) . ^^^ |
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| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| Because of its therapeutic potential , we have constructed a longer acting analog of TSH by fusing the carboxy terminal extension peptide ( CTEP ) of hCG beta onto TSH beta . ^^^ |
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| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| Using site directed mutagenesis we have replaced Tyr 37 in hCG beta , i . e . , the invariant Tyr in all known mammalian CG , LH , FSH , and TSH beta subunits , with two hydrophobic amino acid residues , Phe and Leu . ^^^ |
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| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| The cDNAs encoding Xenopus laevis prolactin ( PRL ) and the alpha and beta subunits of thyroid stimulating hormone ( TSH alpha and TSH beta , respectively ) have been cloned from a pituitary library . ^^^ |
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| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| Immunohistochemical examination revealed that not only TSH beta and TSH alpha but also prolactin and growth hormone synthesizing cells were present in the tumor tissue . ^^^ |
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| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| In studies of three negatively regulated genes ( the pituitary thyroid stimulating hormone alpha subunit [ TSH alpha ] , TSH beta , and hypothalamic thyrotropin releasing hormone [ TRH ] genes ) , stimulation of basal promoter activity by unliganded TR beta was impaired by introducing the P214R CoR mutation . ^^^ |
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| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| In transient transfection assays , the GS 125 mutant abolished transactivation on three classic pTREs ( DR+4 , LAP , and PAL ) and all negatively regulated promoters in the H P T axis ( TRH , TSH beta , and TSH alpha ) . ^^^ |
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| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| METHODS : Thyrotropin in sera was captured by an alkaline phosphase labeled mAb against TSH beta subunit and a FITC labeled mAb against TSH alpha subunit . ^^^ |
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| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| CGA overexpression was not accompanied by overexpression of the CGB , LHB , TSHB or FSHB genes to produce ectopic glycoprotein hormones . ^^^ |
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| Interacting proteins: P01222 and P01215 |
Pubmed |
SVM Score :0.0 |
| To examine the molecular basis for the decreased pituitary growth hormone ( GH ) and thyrotropin ( TSH ) content during restricted feeding , fasting and diabetes , we measured steady state levels of mRNA for TSH alpha , TSH beta and GH in the pituitary from normal rats either fed ad libitum ( C ) , limited to 75 % , 50 % and 25 % ( FR 75 , FR 50 , FR 25 , respectively ) of ad libitum intake , or deprived of food for 2 and 4 days ( F 2 and F 4 , respectively ) , and also in streptozotocin diabetic ( D ) and D insulin treated animals . ^^^ Pituitary mRNA was quantified by Northern blot hybridization with cDNA probes specific for rat TSH alpha , TSH beta and GH . ^^^ Thyroidectomy resulted in a marked increase in both TSH beta and TSH alpha mRNAs , the changes in TSH beta mRNA being greater than those in TSH alpha mRNA . ^^^ In contrast , FR 50 , FR 25 , F 2 , F 4 and D rats exhibited a decrease in pituitary TSH beta mRNA ( 60 % , 50 % , 35 % , 36 % and 33 % , respectively , of C values ; p < 0 . 01 0 . 05 ) and in TSH alpha mRNA levels ( 81 % , 64 % , 46 % , 43 % and 36 % , respectively , of normal values ; p < 0 . 02 0 . 05 ) , TSH beta mRNA showing the greater changes . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^ |
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