| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.5027161 |
| We propose that OT binds to specific OT receptors ( OTR ) on the endometrium to stimulate phosphoinositide ( PI ) hydrolysis , thereby activating the inositol trisphosphate ( IP 3 ) diacylglycerol ( DAG ) second messenger system to promote pulsatile PGF 2 alpha secretion . 0.5027161^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.59082564 |
| Although the interactions between OT and OTR have studied extensively , the relationship to OTase remains to be clarified . 0.59082564^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| The oxytocin receptor ( OXTR ) and its ligand , oxytocin ( OXT ) , regulate reproductive physiology ( i . e . , parturition and lactation ) and sociosexual behaviors . ^^^ To define the essential functions of OXTR , we generated mice with a null mutation in the Oxtr gene ( Oxtr ( / ) ) and compared them with OXT deficient ( Oxt ( / ) ) mice . ^^^ These data suggest a developmental role for the OXT / OXTR system in shaping adult aggressive behavior . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| The influences of visual wulst and optic tract ( OTr ) stimulation on units recorded from the pigeon ' s optic tectum ( OT ) were studied . 127 tectal units were excited with short latency by a single OTr volley . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| The facilitation of sexual receptivity by oxytocin ( OT ) in female rats is related to the regulation of oxytocin receptors ( OTR ) by ovarian steroids in the ventromedial nuclei ( VMN ) of the hypothalamus . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Thus , the OTR is an estrogen induced neurotransmitter receptor that is transported to its site of action , the lateral ventromedial hypothalamus , where it is modulated by P and where estrogen induced OT immunoreactivity is found . . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Injections in area 18 revealed 1 ) zones of discontinuous connections with area 17 that followed a topographic pattern , 2 ) patches of intrinsic connections that spread over distances of up to 6 8 mm from the injection site ; 3 ) two zones of uneven connections with OT cortex suggesting the locations of at least two visual areas , OTr and OTc ; 4 ) connections with limbic cortex rostromedial to areas 17 and 18 ; 5 ) sparse connections with regions of temporal cortex lateral to OT ; and 6 ) uneven callosal connections with area 18 and OT cortex . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| The OT R showed the highest levels during the secretory phase in the isthmus , but showed little change in the ampulla throughout the menstrual cycle . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| We previously cloned a rat OT receptor ( OTR ) gene and showed that its expression in rat uterus results in several transcripts ranging in size from 2 . 9 6 . 7 kilobases . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| The purpose of this study was to explore gene expressions of OT and OT receptor ( OTR ) in human cumulus cells . ^^^ OT and OTR gene expressions were investigated by employing reverse transcription polymerase chain reaction and reverse transcription polymerase chain reaction / single strand conformation polymorphism methods . ^^^ This study is the first to describe the simultaneous expression of both OT and OTR genes in human cumulus cells . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| During the estrous cycle , OTR mRNA levels increased 2 fold between metestrus and proestrus , whereas oxytocin ( OT ) binding rose more than 10 fold within this same interval . ^^^ Treatment of ovariectomized rats with E 2 lead to a significant increase in both OTR mRNA levels ( 4 . 4 fold ) and OT binding ( < 6 fold ) . ^^^ Cotreatment with P 4 strongly reduced OT binding by 75 % ( P < 0 . 01 ) but did not significantly affect the E 2 induced rise in OTR mRNA ( 11 % decrease , P > 0 . 1 ) . ^^^ Our data suggest that the increased expression of OT binding sites observed at the onset of labor and at proestrus is mediated , at least in part , by an E 2 induced up regulation of OTR gene expression . ^^^ However , it also appears that OTR mRNA levels are not the sole determinants of uterine OT binding . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Myometrial contraction induced by oxytocin ( OT ) is dependent on the OT concentration and the number of OT receptors ( OTR ) expressed . ^^^ In the present study , we evaluate the change in the number of OTR expressed on the cell surface after the addition of OT . ^^^ After OT was added under various conditions to myometrial cells , we measured the number of OTR expressed on the cell surface ( surface OTR ) and those in the plasma membrane ( PM ) ( total OTR ) . 1 . ^^^ After the addition of 1 nM OT , total OTR was 25 . 78 fmol / mg protein in the OT negative control and was 23 . 66 and 7 . 88 at 30 minutes and 24 hours . 2 . ^^^ Surface OTR showed no changes after the addition of 1 nM OT at 4 degrees C , and the value after 60 minutes was 8 . 90 fmol / mg protein . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| We demonstrated the enhanced expression of OT receptor ( OTR ) mRNA in chorio decidual tissue , using the polymerase chain reaction after the reverse transcriptase reaction ( RT PCR ) and Northern blot analysis . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| The expression of oxytocin receptor ( OT R ) mRNA in the rat central nervous system was examined by in situ hybridization histochemistry using cRNA probe . ^^^ Wide distribution of cells expressing OT R mRNA was observed not only in the hypothalamus , but also in other regions . ^^^ There were high levels of OT R mRNA in the anterior olfactory nuclei , tenia tecta , olfactory tubercle , rostral most region of the frontal cortex , piriform cortex , layers 2 and 3 of the neocortex , bed nucleus of the stria terminalis , anterior medial preoptic nucleus ( AV3V region ) , magnocellular preoptic nucleus , supraoptic nucleus , paraventricular hypothalamic nucleus , retrochiasmatic nucleus , ventromedial hypothalamic nucleus , paraventricular thalamic nucleus , central amygdaloid nucleus , medial amygdaloid nucleus , posterior cortical amygdaloid nucleus , amygdalohippocampal area , subiculum , prepositus hypoglossal nucleus , and dorsal motor nucleus of vagus . ^^^ However , in the inferior olive nucleus , OT R mRNA was not detected despite an abundance of binding sites showed by receptor binding autography . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| To determine whether the effect of oxytocin ( OT ) on progesterone production in primate luteal tissue may be receptor mediated , we used biochemical procedures to examine the CL of the baboon ( Papio anubis ) for the presence of OT receptors ( OT R ) . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Oxytocin ( OT ) receptors ( OTR ) in rabbit amnion increase more than 200 fold at the end of gestation . ^^^ Judging from the greater effects on PGE 2 release , these results suggest that forskolin and cortisol up regulate the signal response mechanism to OT as well as the OTR concentration . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Eight of 14 paramedian infarctions had complete ocular tilt reaction ( OTR ) with contraversive head tilt , skew deviation , OT , and SVV tilt . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Thus we recently assessed the effects of E and P on OTR expression using cultures of human uterine cells and we examined the changes in the expressed OTRs following treatment of the cell with exogenous OT . ^^^ When 1 nM OT was added to the culture , the total OTR concentration did not change within the first 30 minutes of incubation , but decreased by about 70 % twenty four hours after addition of OT . 2 . ^^^ The concentration of OTRs at the cell surface ( surface OTR concentration ) , as measured in myometrial cells that adhered to the culture plate , did not change with time when the cells were cultured at 4 degrees C in the presence of 1 nM OT . ^^^ However , when cultured at 37 degrees C , the surface OTR concentration showed decreases dependent on the concentration of OT added and the time after the addition of OT . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Although this is considered to reflect OT receptor ( OTR ) augmentation in myometrium , neither spatial expression of OTR nor the level of the receptor message during the course of pregnancy have been investigated at the molecular level . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| OT receptors ( OTR ) in these two species show virtually identical kinetics , specificities , and cDNA sequences ( RNA from parturient uterus ) . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| We have also cloned the rat OT receptor ( OTR ) gene and developed a polymerase chain reaction ( PCR ) based assay to measure OTR mRNA . ^^^ The mechanisms of E induction of the uterine OT gene remain unclear , inasmuch as the OTR gene promoter does not contain a classical estrogen response element ( ERE ) . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| The peptide initiates and maintains myometrial contractions , it stimulates release of PGF 2 alpha from the endometrium and fetal membranes and , as demonstrated in this study , OT induces PGE 2 release from cervical tissues in an OTR dependent manner . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| The distributions of oxytocin ( OT ) and the oxytocin receptor ( OTR ) have been characterised in preovulatory follicles of the marmoset monkey and are described in relation to the process of luteinisation using a combination of in vitro and in vivo techniques , including immunohistochemistry and granulosa cell culture . ^^^ After hCG treatment , almost all granulosa cell layers in antral follicles stained positively for both OT ir and OTR ir , most prominently in preovulatory follicles and especially in the cumulus oophorus . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| The purpose of this study was to examine the evidence for gene expression and localization of OT and OT receptor ( OTR ) in the cumulus cells surrounding the oocytes . ^^^ OT and OTR gene expression was analyzed with reverse transcriptase polymerase chain reaction ( RT PCR ) and RT PCR / single strand conformation polymorphism ( SSCP ) . ^^^ These results are the first observations of simultaneous OT and OTR gene expression in cumulus cells , suggesting that ovarian OT might have some physiological role in the early stage of embryo development . . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| At the end of pregnancy , the myometrium becomes extremely sensitive to oxytocin ( OT ) as result of a dramatic increase in the number of OT receptors ( OTR ) , indicating an important role for OTR in the process of labor . ^^^ Positive staining of OTR in endometrial glandular cells supports the view that OT is involved in PG production by the endometrium in late pregnancy . ^^^ Increased expression of OTR and its mRNA in endometrium may relate to the role of OT in regulating PG production by the endometrium during labor . . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| The expression of the oxytocin ( OT ) receptor ( OTR ) in breast cancer was studied using newly established anti OTR monoclonal antibodies . ^^^ These findings indicated that the OTR is expressed in breast cancer derived not from the myoepithelium but from the glandular or ductal epithelium ; however , the biological function of OT in breast cancer remains to be determined . . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Moreover , they show that region specific regulation of OTR gene expression underlies the previously observed region specific steroid regulation of central OT binding sites . . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| E 2 receptor ( ER ) was measured by enzyme immunoassay , and P 4 receptor ( PR ) and OT receptor ( OTR ) were measured by binding assays . ^^^ Uterine OTR did not increase until the morning of delivery , and uterine OT peptide concentrations increased only during parturition . ^^^ These results support the hypothesis that E 2 stimulates the synthesis of ER , OT , and OTR within the rat uterus and is essential for normal parturition . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Binding studies showed that E 2 treatment also led to an increase in renal OT binding sites , suggesting that the shortened OTR transcripts encoded a functional receptor . ^^^ The differential tissue specific regulation of OTR gene expression may represent a mechanism by which circulating OT can assume a multifunctional role in both reproduction and sodium homeostasis . . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Oxytocin ( OT ) receptor ( OTR ) concentrations were determined in the cervix of nonpregnant cows on cycle Days 0 , 3 , 7 8 , 17 , and 19 ( n = 3 4 cows each day ) ; [ 3H ] OT was used as the labeled ligand . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| We have presented evidence of heterogeneous OTR and 125I P 3 BSA binding sites in the MPOA as well as evidence of extensive interactions of steroids and OT in the MPOA , that can not be adequately explained by genomic effects of steroids . ^^^ We also discuss a putative analogue between steroid control of OTR stimulation of intracellular calcium levels , phospholipase C activity and prostaglandins in the uterus and steroid effects on OT systems in brain . ^^^ We have developed a model for steroid control of both OT release and OTR in which we suggest that steroids and OT bind to membrane receptors coupled to G proteins . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Oxytocin treatment caused a small increase in oxytocin receptor concentration in the luminal epithelium on days 8 and 10 ( 20 + / 4 in Group P and 107 + / 35 fmol mg protein 1 in Group OT , P < 0 . 01 ) but the rise on day 14 was not affected ( 267 + / 82 in Group OT and 411 + / 120 fmol mg protein 1 in Group OTW ) . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| After the RIN cell OTR cDNA was stably transfected into CHO cells ( CHO OTR ) , the cell membranes bound iodinated oxytocin antagonist with an apparent Kd comparable to that of RIN cell membranes and those from other OT target cells . ^^^ Comparison of the ligand specificities of CHO OTR and RIN cells membranes showed that the relative Ki values of a series of OT analogues were approximately equivalent in both preparations . ^^^ Stimulation of CHO OTR cells with OT caused an increase in cytosolic calcium concentration originating from both intracellular and extracellular sources , and a dose dependent increase in inositol phosphate levels . ^^^ The expression of OTR in pancreatic cells implies that OT plays a role in pancreatic function . . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| There was synthesis of both OT and the oxytocin receptor ( OTR ) within the testis , and both were markedly expressed within the Leydig cells . ^^^ Within the prostate , there appears to be no production of OT or neurophysin , though there appears to be weak expression of the OTR in the basal layers of the secretory epithelium . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Collectively , these data suggest that chronic treatment of ewes with estradiol during the cycle can prolong the interestrous interval by reducing uterine concentration of OTr and hence OT induced secretion of prostaglandin . . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| To further investigate the interaction of Gi with the OTR , we showed that OT stimulation of guanosine 5 ' triphosphatase activity in intact myometrial membranes was inhibited by pertussis toxin . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Oxytocin ( OT ) and its receptor ( OTR ) are synthesized in the endometrium and myometrium of the pregnant rat during late gestation . ^^^ The purpose of the present studies was to investigate changes in OT and OTR messenger RNA ( mRNA ) and peptide synthesis within the pregnant rat uterus during RU 486 induced parturition . ^^^ OT , OTR , and estrogen receptor mRNAs were measured with ribonuclease protection assays . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| In contrast , immunoreactivity for the OT receptor ( OTR ) was localized primarily in the basal GC layer . ^^^ After application of exogenous hCG , there was a marked enhancement in both the staining intensity and the number of cells positive for OT and the OTR in all GC layers of antral follicles , especially in the preovulatory follicle . ^^^ These results describing the presence and distribution of OT and OTR and their modulation by hCG , as well as the luteotrophic effect of OT in cultured GC from preovulatory follicles , implicate OT as a paracrine mediator in the luteinization process in the primate ovary . . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| The affinity and specificity of an antagonist of oxytocin , [ 1 D ( CH 2 ) 5 , Tyr ( ME ) 2 , Thr 4 , Tyr NH 2 ( 9 ) ] ornithine vasotocin ( OTA ) , to oxytocin receptors ( OTR ) in bovine gestational endometrium was determined in displacement experiments with oxytocin ( OT ) and vasopressin ( AVP ) analogues and compared to myometrial OTR . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| We evaluated the effects of lateral intracerebroventricular administration of oxytocin ( OT ) and / or a selective oxytocin receptor antagonist ( OTX ) , 1 deamino 2 D Tyr ( OEt ) 4 Thr 8 Orn OT , on ingestion of intraorally delivered 12 . 5 % glucose in rats that were either nondeprived or deprived of food for 20 h . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Oxytocin receptors ( OT R ) are known to be involved in the course of labor since a massive increase in OT binding sites is observed in the uterus just before parturition . ^^^ To determine exactly which subtypes of OT / AVP receptors are expressed in the rat uterus near parturition , we carried out absolute quantitations of the neurohypophysial hormone receptor ( OT R and the vasopressin receptors V1a R , V1b R and V 2 R ) mRNAs with an assay based on reverse transcription polymerase chain reaction ( RT PCR ) using in vitro transcribed mutated cRNAs as internal standards . ^^^ The number of mRNA molecules / ng of total RNA was 35 + / 6 , 220 + / 33 and 39 + / 9 for OT R ( P < 0 . 01 ) and 16 + / 1 , 25 + / 8 and 31 + / 5 for V1a R ( P > 0 . 05 ) on day ( D ) 21 , 22 and 23 of gestation ( post parturient ) , respectively . ^^^ Therefore , the heterogeneity of OT and AVP receptors in the rat uterus can only be assigned to the presence of OT R and V1a R neurohypophysial hormone receptor subtypes , whereas V1b R and V 2 R can not be invoked . ^^^ Only OT R mRNA levels change in the uterus near parturition . . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| These studies were performed to test the hypotheses that : 1 ) endometrial responsiveness to oxytocin ( OT ) in pig endometrium is associated with changes in OT receptor ( OTr ) population density resulting from corresponding regulation of OTr gene transcription , 2 ) endometrial responsiveness to OT is controlled solely through a mechanism involving changes in OTr population density , and 3 ) OTr population density and endometrial responsiveness to OT differ between diestrus and early pregnancy in pigs . ^^^ These results indicate that control of PGF 2 alpha secretion in cyclic pigs appeared to occur primarily at the level of OTr coupling to G protein because changes in OTr number were not associated with increased sensitivity to OT or G protein activation by AIF 4 . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| We showed previously that CHO cells stably transfected with the rat oxytocin receptor ( CHO OTR cells ) also synthesize PGE 2 in response to OT . ^^^ OT treatment caused the rapid phosphorylation of extracellular signal regulated protein kinase 2 ( ERK 2 or p42MAPK ) , which was partially inhibited by pertussis toxin ( PTX ) , consistent with OTR Gi coupling . ^^^ The ERK c fos pathway has been shown to be associated with cell proliferation , but OT had no effect on [ 3H ] thymidine uptake by CHO OTR cells . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| The nona peptide oxytocin ( OT ) induces contraction of the myometrium by interaction with specific plasma membrane associated OT receptors ( OTR ) , whereas stimulation of beta 2 adrenoceptors ( beta2AR ) causes relaxation . ^^^ Treatment with OT had no effect on the amount of myometrial OTR mRNA . ^^^ We have previously found that OT down regulates OTR in the non pregnant rat myometrium , but this therefore does not appear to take place at the level of mRNA production . ^^^ This was accompanied by a 91 % rise in OTR binding and an augmented contractile response of isolated uterine strips to OT , suggesting that the increased production of mRNA reflects formation of active receptors . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Developmental aspects of oxytocin ( OT ) receptors ( OTR ) in uterine tissues before puberty are not known . ^^^ Whole uterus and cervix were taken from third trimester fetuses and at birth . [ 3H ] OT binding and specificity , localization of immunoreactive ( ir ) OTR , OTR messenger RNA , and OT induced release of PGF2alpha were determined . ^^^ The uterus from fetuses and the neonate expressed OTR messenger RNA and bound [ 3H ] OT . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Three experiments were conducted , the objectives of which were to 1 ) examine the effects of exogenous estradiol ( E 2 ) and progesterone ( P 4 ) on uterine concentrations of oxytocin receptors ( OTR ) and OTR mRNA , as well as the effect of exogenous P 4 on progesterone receptors ( PR ) during the late luteal phase of the cycle , and 2 ) ascertain whether chronic E 2 treatment of ewes during the cycle would alter prostaglandin F2alpha ( PGF2alpha ) induced secretion of luteal oxytocin ( OT ) . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Oxytocin ( OT ) stimulated PGE 2 release by rabbit amnion is enhanced by the up regulation of oxytocin receptors ( OTR ) , which increase about 200 fold at the end of pregnancy . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| To determine whether oxytocin ( OT ) could be added to the list of growth factors acting on neoplastic cells of nervous origin , we investigated the presence of oxytocin receptors ( OTR ) in human primary neuroblastomas and glioblastomas and related cell lines . ^^^ In order to clarify whether OT exerts any biological effect on these tumors through OTR , we also studied cell proliferation in 3 human neuroblastoma cell lines ( SK N SH , SH SY5Y , IMR 32 ) and one human anaplastic astrocytoma cell line ( MOG G UVW ) treated with OT 1 nM to 100 nM for 48 and 96 hr . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| These data confirm our previous evidence of a role of OT and OTR in normal and neoplastic breast cells . . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| These effects were mimicked by the oxytocin receptor ( OTR ) agonist [ Thr 4 , Gly 7 ] OT and inhibited by the OTR antagonist desGly NH2d ( CH 2 ) 5 [ D Tyr 2 , Thr 4 ] OVT . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| For over three decades , scientists in a number of different laboratories have worked to design peptide analogues of oxytocin ( OT ) selective for the oxytocin receptor . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Infusion of the OTR antagonist atosiban down regulated OTR binding sites in the parturient myometrium and resulted in an impaired contractile response to OT without affecting gestational length . ^^^ This treatment also down regulated myometrial OTR and weakened the contractile response to OT , but it did not change gestational length . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Vasopressin ( AVP ) and oxytocin ( OT ) are cyclic nonapeptides whose actions are mediated by activation of specific G protein coupled receptors ( GPCRs ) currently classified into V 1 vascular ( V1R ) , V 2 renal ( V2R ) and V 3 pituitary ( V3R ) AVP receptors and OT receptors ( OTR ) . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| A chimeric OT / V2 receptor including OT receptor ( OTR ) sequences from its N terminus to the middle of transmembrane region three showed both high affinity OT binding ( Ki = 3 nM ) and activation of the adenylyl cyclase . ^^^ These results indicate ( 1 ) that the third and / or the fourth intracellular domain of the V2R are involved in G protein coupling and ( 2 ) for high affinity OT binding the N terminal third of the OTR plays an important role . ^^^ By detailed binding studies on a second series of chimeric V2 / OT receptors with AVP , OT and the two hybrid hormone derivatives arginine vasotocin and oxypressin it was further demonstrated that the first two extracellular domains of the OTR are involved in binding to the C terminal tripeptide of OT . ^^^ Moreover , the third extracellular domain of the OTR is able to contact the cyclic part of OT and the fourth outer domain does not interact with the two variable amino acid residues of AVP and OT . ^^^ Thus , the first three extracellular domains of the OTR provide an essential part of the OT binding site . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| The purpose of this study was to determine the specificity and concentration of oxytocin ( OT ) and arginine vasopressin ( AVP ) binding sites in non pregnant ( NP ) human and rhesus monkey endometrium , myometrium and fibromyomas , and to determine the cellular localization of OT receptor ( OTR ) . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Vasopressin ( VP ) and oxytocin ( OT ) are cyclic nonapeptides whose actions are mediated by stimulation of specific G protein coupled receptors ( GPCRs ) currently classified into V 1 vascular ( V1R ) , V 2 renal ( V2R ) and V 3 pituitary ( V3R ) VP receptors and OT receptors ( OTR ) . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Serum / DEX up regulation of OTR restored the responsiveness to OT . ^^^ In addition to showing that OTRs in the breast tumor cells are functional , these studies show that Hs578T cells can be used to study molecular regulation of OTR gene expression and intracellular signaling pathways stimulated by OT . . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| We showed the requirement for GABP alpha / beta and c Fos / c Jun in endogenous OTR gene expression , using oligonucleotide GABP and AP 1 binding decoys to inhibit serum induced increases in 125I labeled OT antagonist binding to Hs578T cells . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| In vivo experimentation implies estrogenic control of both the oxytocin ( OT ) gene and that for its receptor ( OTR ) . ^^^ The current consensus suggests that the sex steroids are acting indirectly on both the OT and OTR genes , possibly involving intermediate transcription factors or cofactors . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Our recent studies have shown that regulation of uterine oxytocin ( OT ) binding involves at least two different mechanism : Estradiol ( E 2 ) induced upregulation is accompanied by an increase in OT receptor ( OTR ) mRNA accumulation , implying that the E 2 effect is mediated via increased OTR gene transcription and / or OTR mRNA stabilization . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| The present study describes the use of nose poke habituation as a memory task in mice and demonstrates that it is sensitive to oxytocin ( OT ) and an oxytocin receptor antagonist ( AOT ) administered after the learning trial . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Furthermore , OT receptor ( OTR ) mRNA was found in the vena cava , pulmonary vein , and pulmonary artery with lower levels in the aorta , suggesting vessel specific OTR distribution . ^^^ In addition , we have demonstrated that diethylstilbestrol treatment of immature female rats increased OT significantly in the vena cava but not in the aorta and augmented OTR mRNA in both the aorta ( 4 fold ) and vena cava ( 2 fold ) , implying regulation by estrogen . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Because the sequence of OC derived hexapeptide ( HP ) is nearly identical to the E 2 region of the oxytocin receptor ( OTR ) , we set out to ascertain whether the HP interferes with the osteosarcoma ( OS ) associated oxytocin ( OT ) system . ^^^ We documented the presence and functional activity of OTRs in several OS cells by means of ( a ) OT mediated inhibition of OS growth ; ( b ) expression of OTR mRNA by means of reverse transcription PCR ; ( c ) immunofluorescence staining with IF 3 monoclonal antibody specific for human OTR ; and ( d ) saturation binding and Scatchard analysis of OT binding to the receptors of isolated membranes or intact OS cells . ^^^ The results indicate the existence of an OTR / OT system in tumor cells of bone origin . ^^^ Suggested plasminogen activator OC OTR / OT interactions may have an effect on the regulation of cell proliferation within the bone tissue as well as properties of the extracellular matrix surrounding the tumor foci in the bone . . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Uterine explants were incubated in vitro to determine the effects of IL 6 on uterine synthesis of oxytocin ( OT ) and its receptor ( OTR ) . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| This is the first report of OTR mRNA and protein expression and of prescribed OT signal pathways in osteoblastic cells . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Subsequently , the OTR complexes with a selective agonist [ Thr 4 , Gly 7 ] OT , a selective cyclohexapeptide antagonist L 366 , 948 and oxytocin itself were modeled and relaxed using a constrained simulated annealing ( CSA ) protocol . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| This study was therefore designed to search for OTR expression and the OT effect in endometrial carcinomas . ^^^ Moreover , in a human endometrial carcinoma cell line ( COLO 684 ) OTR was demonstrated by immunofluorescence and RT PCR and it was observed that OT treatment ( 10 ( 11 ) 10 ( 7 ) M ) significantly inhibited cell proliferation . ^^^ In conclusion , this study presents evidence of OTR expression in human endometrial carcinomas and of an OT antiproliferative effect on human endometrial cancer cells in vitro . ^^^ It is further suggested that OT and OTR may be involved in the regulation of endometrial cells , not only in physiological conditions but also in a neoplastic context . . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| OTR functionality was demonstrated by measuring the OT induced increase in the intracellular calcium concentrations . ^^^ Finally , we observed a dose dependent OT stimulation of cell proliferation associated with OTR activation that was completely abolished by the selective OT antagonist . ^^^ These findings provide the first evidence of the presence of functional OTR in normal trophoblast cell lines as well as in choriocarcinoma cells and show that a specific effect of OT on normal and neoplastic trophoblast is to promote cellular proliferation . . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : To review the potential roles of oxytocin ( OT ) and its receptor ( OTR ) in the regulation of parturition . ^^^ RESULTS : The literature provides strong support for a role for the OT / OTR system in the regulation of human parturition . ^^^ CONCLUSION : The OT / OTR system provides a potentially important therapeutic target for regulating the timing of human birth . . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| At term , uterine epithelial cells express oxytocin ( OT ) as well as the OT receptor ( OTR ) . ^^^ We have studied the subcellular localization of OT like immunoreactivity ( OT IR ) and OTR IR in rat uterine epithelium by immuno gold labelling of ultrathin frozen sections . ^^^ Our observations indicate that OT and OTR are both distributed preferentially to the apical surface of rat uterine epithelial cells . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Here , we structurally characterized OT receptor ( OTR ) mRNA in mammary gland and analyzed its expression during gestation and lactation and in response to steroid treatment . ^^^ During pregnancy , OTR mRNA per mammary gland increased approximately 150 fold and remained high during lactation , consistent with the previously identified regulation of OT binding sites and the role of OT during lactation . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| The results indicate that OT receptors ( OTR ) but not alpha ( 1 ) AR are linked to myometrial PLC activation and uterine contraction in late pregnant and parturient mouse . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| The increased OTR mRNA may serve as a mechanism to increase uterine sensitivity to OT during late gestation . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| We examined the effect of neurons on oxytocin ( OT ) receptors ( OTR ) and OTR gene expression in cultured astrocytes . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| In this context , OT acts as a growth regulator . through the activation of specific G coupled transmembrane receptors ( OTR ) . ^^^ In vitro , an antiproliferative effect of OT was demonstrated in neoplastic cells of either epithelial ( mammary and endometrial ) or nervous or bone origin , all expressing OTR . ^^^ The unexpected role of OT ( and OT analogues ) in regulating cell proliferation , as well as the diffuse expression of OTR in neoplastic tissue of different origin , open new perspectives on the biological role of the OT OTR system in cancer . . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Third , the presence of functional OTRs was evidenced by showing that agonist substances having a high affinity for the human OTR , namely OT , AVP and [ Thr ( 4 ) Gly ( 7 ) ] OT , increased the rate of myoblasts fusion and myotubes formation in the cultures , whereas F 180 , a 5 ( 1a ) R selective agonist , and dDAVP , a 5 ( 2 ) R agonist had no significant effect on the fusion process . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| OT and its receptor ( OTR ) have been identified in the rat heart and great vessels . ^^^ Expression of OT and OTR is increased in some tissues during pregnancy . ^^^ We hypothesized that the OT / OTR system may be a physiological regulator of vascular tone and mediate the decreased vascular resistance noted during pregnancy . ^^^ Although OTR mRNA was identified by RT PCR , OT did not elicit a vasodilatory effect in any of the vessels studied . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| The effects of the peptide hormone oxytocin ( OT ) are mediated by the oxytocin receptor , which is a member of the G protein coupled receptor family . ^^^ Previous reports have indicated that the N terminus of the OTR is required for OT binding . ^^^ The aim of this study was to identify which individual residues within the N terminal domain of the human OTR provided these OT binding epitopes . ^^^ Although a number of residues within the OTR will be required for optimal OT OTR interaction , our data establish that Arg ( 34 ) within the N terminal domain contributes to high affinity OT binding . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| The aim of this study was to evaluate the relationship between the occurrence of retention of the fetal membranes ( RFM ) and the hormonal concentrations of progesterone , estradiol 17beta , prostaglandin E ( 2 ) ( PGE ( 2 ) ) , prostaglandin F ( 2alpha ) ( PGF ( 2alpha ) ) , oxytocin ( OT ) , oxytocin receptor ( OT R ) , endothelin 1 and angiotensin 2 ( Ang 2 ) in the placental tissues of cattle . ^^^ In maternal tissues , the OT and OT R concentrations in the RFM group were lower than those at 0 and 6 h after parturition in the non RFM group . ^^^ In conclusion , the present results suggest that ET 1 and Ang 2 may play differential tissue specific roles in the placental unit that may amplify the local endocrinological cascade involving OT , OT R and PGF ( 2alpha ) interactions which are necessary for normal placental separation in the cow . . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| We recently discovered the existence of the oxytocin / oxytocin receptor ( OT / OTR ) system in the heart . ^^^ In addition , OT as well as DMSO increased OTR protein and OTR mRNA , and OTA completely inhibited the formation of cardiomyocytes in OT and DMSO supplemented cultures . ^^^ These results suggest that the OT / OTR system plays an important role in cardiogenesis by promoting cardiomyocyte differentiation . . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Oxytocin ( OT ) inhibits the proliferation of MCF 7 estrogen dependent human breast cancer cells , via specific OT receptors ( OTR ) . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| In that view , OT receptor ( OTR ) expression , concomitant with OT synthesis and secretion , was evidenced on three different SCCL cell lines ( DMS 79 , H 146 , and H 345 ) and related to the vasopressin ( VP ) system . ^^^ Specific OT , VP , OTR , V1a VP receptor ( V1aR ) , and V1b / V3 VP receptor ( V1bR / V3R ) transcripts were identified by reverse transcription PCR in all cell lines studied . ^^^ Selectivity of 125I OVTA binding was confirmed by displacement curves obtained with various OTR and VP receptor agonists and antagonists ( OT , OVTA , L 371 , 257 , VP , F 180 ) . ^^^ Immunocytochemistry identified cellular OT and VP , and peptide secretion was measured in supernatants of SCCL cultures . [ 3H ] Thymidine incorporations , applied on H 345 cells , demonstrated a dose dependent mitogenic effect of exogenous OT ( 1 and 100 nM ) that was abolished by the OTR antagonist OVTA . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| The signaling between the OTR and the SERT was analyzed by measuring the extent of inhibition of OT and PMA ( activator of protein kinase C on 5HT uptake and the capability of Ro 318220 ( specific inhibitor of PKC ) to increase 5HT uptake and block the effect of the above compounds in mast cells . ^^^ The results showed that in murine peritoneal mast cells in vitro ( 1 ) ovarian hormones modulate OTR but not SERT expression , ( 2 ) the magnitude of OT action on 5HT uptake depends on the number of OTRs expressed in mast cells , and ( 3 ) the signaling between OTR and the SERT is mediated through the activation of protein kinase C . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| To study the physiological role of these OTR , the effect of OT on the release of PGE , from the cervical mucosa of periestrous cows in vivo was determined by inserting bags made of dialysis tubing containing isooncotic saline solution in the endocervix for two 2 h periods , a fresh bag for each period . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Our data indicate that cholesterol acts as an allosteric modulator of the oxytocin receptor and stabilizes both membrane associated and solubilized OT receptors in a high affinity state for agonists and antagonists . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Atosiban , an oxytocin receptor antagonist , effectively attenuated the OT induced inhibition of gastric emptying . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| The OTR selective agonist [ Thr 4 , Gly 7 ] OT , the OTR selective cyclohexapeptide antagonist L 366 , 948 and OT itself were docked via genetic algorithm to OTR , V1aR , and V2R and relaxed using a constrained simulated annealing protocol . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Their main activities include the urine concentration ( via stimulation of AVP V 2 receptors , V2R , in the kidneys ) , blood pressure regulation ( via stimulation of vascular V1a AVP receptors , V1aR ) , ACTH control ( via stimulation of V1b receptors , V1bR , in the pituitary ) and labor and lactation control ( via stimulation of OT receptors , OTR , in the uterus and nipples , respectively ) . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| In this study we investigated on the presence and function of OT receptor ( OTR ) in rabbit and human epididymis . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Atosiban , an oxytocin receptor antagonist , effectively attenuated the OT induced inhibition of gastric emptying and gastrointestinal transit . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| OT receptor ( OTR ) signals predominantly through Galpha ( q ) , but ERK1 / 2 phosphorylation ( ERK1 / 2 P ) in PHM 1 myometrial cells was not eliminated by inhibition of downstream effectors such as phospholipase C or protein kinase C . ^^^ In both COSM 6 cells overexpressing OTR ( OTR COSM 6 ) and in PHM 1 cells , the epidermal growth factor receptor ( EGFR ) tyrosine kinase inhibitor AG 1478 markedly reduced OT induced ERK1 / 2 P , whereas the platelet derived growth factor receptor tyrosine kinase inhibitor AG 1296 had no effect . ^^^ Furthermore , OT increased EGFR tyrosine phosphorylation in OTR COSM 6 cells , which was inhibited by AG 1478 or EGTA plus thapsigargin pretreatment . ^^^ In both OTR COSM 6 and PHM 1 cells , EGTA reduced OT stimulated ERK1 / 2 P ; no ERK1 / 2 P was observed when intracellular calcium increases were blocked by pretreatment with thapsigargin plus EGTA . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Our recent studies indicate that OT receptors ( OTR ) are present in rabbit and human epididymis and mediate contractility . ^^^ In fact , in an experimental model of hypogonadotropic hypogonadism ( hypo ) induced in rabbits , estrogens , but not androgens , fully restored OT induced epididymal contractility , up regulating OTR gene and protein expression . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : In the present study , we localized oxytocin ( OT ) and its receptor ( OTR ) in the rat aorta , and investigated whether genistein , an isoflavonic phytoestrogen , influences their expression in ovariectomized ( OVX ) rats deficient in estrogen . ^^^ OT and OTR immunostaining was concentrated in the aortic tunica intima , suggesting their paracrine / autocrine action within endothelial cells . ^^^ Reverse transcription polymerase chain reaction analysis showed that 1 and 5 microg / g but not 0 . 1 microg / g genistein elevated OT mRNA ( 2 fold P < 0 . 05 ) , OTR mRNA ( 2 . 5 fold , P < 0 . 05 ) and endothelial nitric oxide synthase ( eNOS ) mRNA ( 2 fold , P < 0 . 05 ) in the aorta of OVX rats . ^^^ In contrast , the treatment of OVX rats with E ( 2 ) elevated ERbeta mRNA ( 1 . 5 fold , P < 0 . 05 ) but similarly to genistein increased OT , OTR , eNOS and ERalpha mRNA . ^^^ CONCLUSION : These results provide the first evidence of OT and OTR co localization in endothelial cells . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| The day after OT injection , oxytocin receptors ( OTR ) were found in the uterine tissues of all animals studied . ^^^ In conclusion , E 2 and P 4 are involved in both PGF 2 synthesis / secretion and OTR formation , however , full PGF response to OT does not develop before puberty . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| The N terminus of the related OT receptor ( OTR ) could recover agonist binding in a chimaeric OTR ( N ) 5 ( 1a ) R construct . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| In this study we investigated the spatial and temporal relationship among oxytocin ( OT ) , oxytocin receptor ( OTR ) , and estradiol ( E 2 ) at term , with ( LAB ) and without labor ( NIL ) , in human amnion ( AM ) , chorio decidua ( CD ) , fundal ( FU ) , and lower segment ( LS ) myometrium . ^^^ In contrast to OT and E 2 the OTR is spatially regulated , with significantly greater expression in the fundal region of the uterus . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Oxytocin receptors ( OTR ) have been described in a number of tumours of different origin , and represent a new target for specific radiolabelled oxytocin ( OT ) analogues in cancer diagnosis and therapy . ^^^ By linking the DOTA chelating agent to position 8 of the deamino derivative of Lys ( 8 ) vasotocin ( dLVT ) , we obtained a new compound ( DOTA dLVT ) with the following characteristics : ( 1 ) it forms a monomeric and stable compound that binds to OTR with an affinity comparable to that of the endogenous OT ligand ; ( 2 ) it is characterised by a very good selectivity profile for the human OTR , with a low affinity binding to the closely related V1a , V1b and V 2 vasopressin receptor subtypes ; ( 3 ) it induces rapid and persistent receptor internalisation and ( 4 ) when radiolabelled , [ ( 111 ) In ] DOTA dLVT is efficiently and selectively taken up by OTR positive tumours grown in mice . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Oxytocin ( OT ) as well as its receptor ( OTR ) play a key role in the process of ( preterm ) labor as part of a paracrine system that regulates myometrial contractility . ^^^ Binding of OT to its corresponding receptor , OTR , leads to activation of actin myosin interactions and therewith myometrial contractions as well as production of intrauterine prostaglandins ( PGE ( 2 ) , PGF ( 2 alpha ) ) mainly in decidua and myometrium . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| The hypothalamic nonapeptide oxytocin ( OT ) has an established role as a circulating hormone but can also act as a neurotransmitter and as a neuromodulator by interacting with its central OT receptor ( OTR ) . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| SSR126768A ( 4 chloro 3 [ ( 3R ) ( + ) 5 chloro 1 ( 2 , 4 dimethoxybenzyl ) 3 methyl 2 oxo 2 , 3 dihydro 1H indol 3 yl ] N ethyl N ( 3 pyridylmethyl ) benzamide , hydrochloride ) : a new selective and orally active oxytocin receptor antagonist for the prevention of preterm labor . 4 chloro 3 [ ( 3R ) ( + ) 5 chloro 1 ( 2 , 4 dimethoxybenzyl ) 3 methyl 2 oxo 2 , 3 dihydro 1H indol 3 yl ] N ethyl N ( 3 pyridylmethyl ) benzamide , hydrochloride ( SSR126768A ) , a new potent and selective , orally active oxytocin ( OT ) receptor antagonist was characterized in several biochemical and pharmacological models . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| In some cases , OT exhibits an oxytocin receptor ( OTR ) mediated antiproliferative effect on cancer cells . ^^^ We demonstrated OTR expressions in the large majority of ovarian carcinoma tissues , and OT inhibited not only proliferation but also migration and invasion in ovarian carcinoma cells in vitro . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| This study has localised oxytocin receptor ( OTR ) mRNA expression within the cervix of non pregnant ewes and related this to changes in the sensitivity of the cervical musculature to administered oxytocin ( OT ) during the oestrous cycle by recording electromyographic ( EMG ) activity . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Oxytocin ( OT ) and the oxytocin receptor ( OTR ) seem to be less important for uterine contractility associated disorders of the non pregnant uterus compared to the pregnant uterus . ^^^ In the present study , we investigated the mutual dependence of OTR , OT and 17beta estradiol ( E ( 2 ) ) with regard to the localization of OTR in the non pregnant uterus . ^^^ Stimulation with E ( 2 ) and OT led to significantly higher OTR gene expression than stimulation with OT alone . ^^^ The data presented demonstrate that the dynamics of OTR expression can be modulated by stimulation with E ( 2 ) and OT , not only in the pregnant but also in the non pregnant uterus . . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| In murine fetal thymic organ cultures , a specific OTR antagonist does not modify the percentage of T cell subsets , but increases late T cell apoptosis further evidencing the involvement of OT / OTR signaling in the control of T cell proliferation and survival . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| This literature review summarizes recent potential evidence , most of which is at the molecular / mechanistic level , in support of Hollander ' s hypothesis that excess oxytocin ( OT ) , possibly through OT administration at birth , could contribute to the development of autistic spectrum disorders and related syndromes by proposed down regulation of the OT receptor ( OTR ) . ^^^ In this review , recent molecular evidence for OTR internalization by excess OT is related to OT ' s reported effects on animal social behavior , favoring social bondage , notably in sheep , voles , rats and especially mice . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Here , we show a maximal OT and OTR protein level in the developing rat heart at day 21 of gestation and postnatal days 1 4 , when cardiac myocytes are at a stage of intense hyperplasia . ^^^ In vivo , the OT / OTR system in the fetal heart was sensitive to the actions of retinoic acid ( RA ) , recognized as a major cardiac morphogen . ^^^ The decline of cardiac OT expression from infancy to adulthood of the rat and changes in cell types expressing OTR indicate a dynamic regulation of the OT system in the heart rather than constitutive expression . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| When expressed at equal concentrations , OTR H7 . 62 / A and OTR Q7 . 66 / A stimulated phosphatidylinositide turnover as well as OTR , whereas OTR H7 . 59 / A , OTR E7 . 63 / A , and OTR R7 . 67 / A exhibited an impaired ability to respond to OT . ^^^ While OTR ( H7 . 62 , Q7 . 66 ) / A exhibited PLC activation equivalent to that of OTR , receptors with two or more changes in H7 . 59 , E7 . 63 , and R7 . 67 lost the ability to respond to OT in a dose dependent manner . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| It is known that the oxytocin ( OT ) and vasopressin ( VP ) genes , normally transcriptionally restricted in their expression , are activated in small cell lung cancer ( SCLC ) , concomitantly with expression of their receptors ( OTR , V1aR , V1bR / V3R and V2R ) . ^^^ Known agonists for OTR ( [ Thr 4 , Gly 7 ] OT ) and V1aR ( F 180 ) , in addition to OT and VP , were able to elicit increases in cytosolic Ca2+ levels and this effect could be blocked using an OTR antagonist ( OVTA ) or a V1aR antagonist ( SR 49059 ) respectively . ^^^ In conclusion , the results presented here indicate that OT and VP induced mitogenic effects on SCLC are respectively mediated by OTR and V1aR signalling and that this mitogenic signalling passes through the phosphorylation of ERK1 / 2 and p90RSK in a PLC , Ca2+ , PKC and MEK1 / 2 dependent pathway . . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| The oxytocin ( OT ) receptor ( OTR ) mediates a wide spectrum of biological actions and is expressed in a large number of different tissues , including uterine , breast , and lung tumors . ^^^ Dose response curves in myometrial cells expressing the endogenous OTR indicated a significant effect of OT on eukaryotic elongation factor 2 dephosphorylation at 1 nM , a concentration close to the dissociation constant ( K ( d ) ) of OT . ^^^ These findings establish a novel link between OTR activation and cellular protein synthesis and thus define a mechanism by which OT assumes a so far unrecognized , physiologically relevant trophic function . . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| However , the level of OT receptor ( OTR ) transcripts and proteins was equivalent at the two phases . ^^^ These results suggest that OT induced uterine contraction is not only regulated by the quantity of OTR in the non pregnant mouse uterus . . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| In order to determine whether OTR might mediate a biological effect of oxytocin ( OT ) in prostate cancer cells , OTR expression was studied by RT PCR and immunofluorescence technique in the human androgen independent prostate cancer cell line DU 145 . ^^^ The data obtained showed that DU 145 cells expressed OTR , whereas no OT mRNA was detected . ^^^ Altogether , the data indicate that the OT / OTR system could be involved in the control of prostate neoplastic pathology . . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| From all OT and VP receptors , only OTR was expressed by all T cell subsets , while V1bR was found in double positive and single positive CD 8 cells . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Binding studies using [ 125I ] oxytocin antagonist ( [ 125I ] OTA ) in cultured human fetal penile smooth muscle cells ( hfPSMC ) revealed the presence of specific OTR with a high capacity and affinity for oxytocin ( OT ) and for OTA . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| The neurohypophyseal hormone oxytocin ( CYIQNCPLG NH ( 2 ) , OT ) is involved in the control of labor , secretion of milk and many social and behavioral functions via interaction with its receptors ( OTR ) located in the uterus , mammary glands and peripheral tissues , respectively . ^^^ In this paper we propose the interactions responsible for OT binding and selectivity to OTR versus vasopressin ( [ F 3 , R 8 ] OT , AVP ) receptors : V1aR and V2R , all three belonging to the Class A G protein coupled receptors ( GPCRs ) . ^^^ The nonconserved hydrophobic residues were proposed as responsible for OTR OT selectivity and ligand recognition . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| In this study , the interactions responsible for AVP binding to vasopressin V1a and V 2 receptors versus the closely related oxytocin ( [ I 3 , L 8 ] AVP , OT ) receptor ( OTR ) have been investigated . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| In rat corpus cavernosum strips , oxytocin ( OT ) and an OTR selective agonist ( [ Thr 4 , Gly 7 ] OT ) induced identical increases in tension , while different vasopressin agonists were less active . ^^^ The OTR antagonist , atosiban , counteracted the contractile effect of OT both in vitro and in vivo . ^^^ Our data showed that OTR is present in the rat penis and mediates contractility both in vitro and in vivo , therefore suggesting a role for OT in maintaining penile detumescence . . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| We previously reported that the oxytocin ( OT ) receptor ( OTR ) mediates an estrogen dependent increase in epididymal contractility . ^^^ Two structurally distinct OTR antagonists [ ( d ( CH 2 ) 5 ( 1 ) , Tyr ( Me ) ( 2 ) , Orn ( 8 ) ) OT and atosiban ] almost completely abolished ET 1 contractility , without competing for [ 125I ] ET 1 binding , suggesting that OT / OTR partially mediates ET 1 action . ^^^ Hence , we propose that an ET 1 induced OT / OTR system activation underlies the estrogen dependent hyperresponsiveness to ET 1 . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Availability of the reelin haploinsufficient ( + / ) reeler mouse ( HRM ) provides a model for examining the role of reelin in the development of the OT system and especially in the expression of the OT receptor ( OTR ) . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| MATERIAL / METHODS : Actions of OT on HUVECs transfected with a copy of the human P LAP gene were therefore examined with a focus on changes in parameters linked to OTR such as calcium mobilization , NO production and cell proliferation . ^^^ Conclusions : These findings suggested that P LAP on the plasma membrane in HUVECs regulates the effects of OT with resolution around OTR . . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Vasopressin ( AVP ) and oxytocin ( OT ) are cyclic nonapeptides whose actions are mediated by the stimulation of specific G protein coupled receptors ( GPCRs ) currently classified into 5 ( 1 ) vascular ( 5 ( 1 ) R ) , 5 ( 2 ) renal ( 5 ( 2 ) R ) and 5 ( 3 ) pituitary ( 5 ( 3 ) R ) AVP receptors and OT receptors ( OTR ) . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| The role of the neurohypophyseal peptide oxytocin ( OT ) and its receptor ( OTR ) in the breast has been described mainly in relation to breast feeding or to neoplastic growth regulation . ^^^ Both epithelial and myoepithelial cells contained the mRNA for OT and OTR ; however , only myoepithelial cells showed an effective OT synthesis and detectable peptide release in the culture medium . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| Central oxytocin receptors ( OTR ) may be involved in adaptations of the brain oxytocin ( OT ) system during gestation , which are critical for systemic release of OT during parturition and lactation . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| The objectives of the study were : ( 1 ) to compare OT receptors ( OTr ) concentrations in endometrium and myometrium of cyclic and early pregnant pigs , ( 2 ) to examine the effect of OT on plasma PGF ( 2 ) alpha secretion during the progressive luteal regression , ( 3 ) to ascertain the effect of OT on inositol phosphates ( IPs ) accumulation in endometrial and myometrial cells of cyclic and early pregnant pigs . ^^^ In summary : ( 1 ) concentrations of OTr on both the endometrium and myometrium were the highest during perioestrus period in pigs , ( 2 ) myometrium of early pregnant sows possessed functional OTr , ( 3 ) oxytocin increased plasma PGFM concentration after initiation of luteolysis ; and ( 4 ) OT stimulated accumulation of IPs in myometrial , but not in endometrial cells . ^^^ In conclusion , OT appears to not be involved in the initiation of luteal regression in sows and functional OTr are still present in the myometrium during early pregnancy ( days 14 16 ) . . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| The investigation of oxytocin receptor ( OTR ) regulation will benefit labor management , because the clinical practice of continuous 4 infusion of OT is not optimal . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| The effects of centrally administered OT in the FPT were blocked with peripheral administration of a brain penetrant OT receptor ( OTR ) antagonist WAY 162720 ( 30 mg / kg i . p . ) , and the effects of peripherally administered OT were blocked with central administration of a non penetrant OTR antagonist L 371 , 257 , suggesting OT acts centrally . ^^^ |
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| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| OTR ( but not a V 1 receptor ) antagonist blocked OT effects without blocking the excitatory effect of PGE 2 . ^^^ |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01178 and P30559 |
Pubmed |
SVM Score :0.0 |
| NA |
|