Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P01138 and P08138	Pubmed	SVM Score :1.0174284
In addition , NGF specifically binds to NGFR on Schwann cells although the biological significance of this binding is not known . 1.0174284^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.590982
Several lines of evidence suggest that NGF is also the ligand for trkE ; ( 1 ) normal human keratinocytes bind NGF with high affinity , ( 2 ) NGF stimulates keratinocyte growth in an autocrine fashion , ( 3 ) NGF exerts its biological effect on keratinocytes through the stimulation of a trk specific tyrosine kinase , and ( 4 ) keratinocytes lack trkA but do express large amount of trkE . trkE might also be the NGF receptor by other human peripheral tissues , such as pancreatic islets , and might represent a non neuronal receptor for this ligand . . 0.590982^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.8338844
Furthermore , a NGF stimulated purine analogue sensitive kinase activity was found to coimmunoprecipitate with p75NTR . 0.8338844^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :1.1953799
Here we show by co immunoprecipitation that NGF activates a protein kinase that is directly associated with p 75 ( NGFR ) in dorsal root ganglion ( DRG ) cells and PC 12 cells in culture . 1.1953799^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.65840269
In another study , the p75NTR was shown to induce apoptotic death of maturing oligodendrocytes when exposed to NGF in vitro . 0.65840269^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.61705061
The pro form of nerve growth factor ( pro NGF ) has been shown to be a high affinity ligand for p75NTR and to induce apoptosis through this receptor . 0.61705061^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.71733957
Furthermore , p75NTR appears to play functional roles that are unrelated to its ability to bind NGF and pro NGF , some of which are ligand independent and others of which are dependent upon binding to other neurotrophins . 0.71733957^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The absence of differences in peptide maps generated from the two sizes of NGF receptor proteins together with structural and binding data strongly indicates that the 200 kDa NGF receptor protein is a complex , probably a dimer , consisting of two 80 kDa NGF receptor proteins associated with a single beta NGF dimeric molecule . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The spatial and temporal pattern of beta NGF receptor expression in the developing chick embryo . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Non neuronal drg cells obtained by a procedure designed for the preparation of pure Schwann cells , as well as RN 6 Schwannoma cells , were beta NGF receptor positive . ^^^ The beta NGF receptor positive non neuronal drg cells displayed behaviour typical of Schwann cells in their interaction with drg neurones in single cell , as well as explant cultures . ^^^ Three stages of neurone Schwann cell interaction were discernible : ( 1 ) association neurites preferentially grew over beta NGF receptor positive non neuronal cells ; ( 2 ) cell division / alignment beta NGF receptor positive non neuronal cells were induced to proliferate and aligned and elongated along neurites ; ( 3 ) ensheathment the outline of beta NGF receptor positive non neuronal cells and neurites merged . ^^^ In drg cell cultures prepared from embryonic stages E 6 E10 , 25 40 % of the non neuronal cells were beta NGF receptor positive . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The 192 IgG increases the apparent affinity of beta NGF binding to fast receptors on PC 12 cells at low temperature by a factor of 2 . 5 to 4 fold and enhances the photoactivatable cross linking of beta NGF to the same receptor while decreasing the cross linking of beta NGF to the slow NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We have solubilized the NGF receptor of adult rabbit superior cervical ganglia ( SCG ) with the nonionic detergent Triton 10 100 . ^^^ Gel chromatography of the receptor or the 125I labeled NGF receptor complex on a column of Sepharose 6B indicated , in both cases , a single component of an apparent hydrodynamic radius of 71 + / 5 A . ^^^ In parallel investigations , we have confirmed the similarity between the hydrodynamic size of the NGF receptor of rabbit SCG and that of the insulin receptor of IM 9 lymphocytes evaluated by similar methods . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The physical properties of the detergent extracted NGF receptor were assessed by Sepharose 6B chromatography and sucrose density gradient ultracentrifugation studies in H2O and D2O . ^^^ The predominant form of the NGF receptor has a Stokes radius of 71 A , a partial specific volume of 0 . 74 ml / g , a sedimentation coefficient of 4 . 3 S , and a frictional ratio of 1 . 8 . ^^^ From these parameters , it can be calculated that the NGF receptor in Triton 10 100 is a minimally hydrophobic , highly asymmetric , intrinsic membrane protein with a molecular weight of approximately 135 , 000 . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Low concentrations of phospholipase A from Vipera russelli decrease NGF receptor binding by lowering the number of binding sites , while phospholipase A preparations from Crotalus terrificus terrificus and bee venom do not affect binding . ^^^ NGF receptor binding appears to be absolutely dependent upon calcium ion . ^^^ NGF receptor sites can be protected from 2 hydroxy 5 nitrobenzyl bromide by incubation with NGF . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
A significant overlap of neuronal populations known to express the low affinity NGF receptor ( LNGFR ) with populations containing aFGF immunoreactivity was also observed . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Ciliary neurotrophic factor prevents neuronal degeneration and promotes low affinity NGF receptor expression in the adult rat CNS . ^^^ CNTF is also involved in up regulation of immunostainable low affinity NGF receptor ( LNGFR ) in cholinergic medial septum and neostriatal neurons and in a population of lateral septum neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NGF receptor containing cortical neurons were not observed in young adults ( less than 50 years old ) and were observed only infrequently in non demented elderly individuals ( 50 80 years old ) . ^^^ In contrast , numerous NGF receptor containing cortical neurons were seen in AD patients of all ages and in one 98 year old nondemented patient . ^^^ In advanced age and AD , numerous NGF receptor positive neurons were located within laminae 2 6 of temporal association cortices whereas only a few were seen in the subicular complex , entorhinal cortex , parahippocampal gyrus , and amygdaloid complex . ^^^ In contrast to AD , NGF receptor containing cortical neurons were not observed in Parkinson disease , Pick disease , or Shy Drager syndrome . ^^^ The NGF receptor containing cortical neurons seen in advanced age and AD were similar in morphology to those observed in human fetal cortex . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Spatiotemporal patterns of expression of NGF and the low affinity NGF receptor in rat embryos suggest functional roles in tissue morphogenesis and myogenesis . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In this report , we demonstrate that K252a is a potent inhibitor ( IC 50 of 3 nM ) of the tyrosine protein kinase activity of the NGF receptor gp140trk , the product of the trk protooncogene . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Two distinct nerve growth factor receptor ( NGFR ) complexes are present on NGF responsive cell types ; these correspond to 100 kDa and 158 kDa for the fast ( fNGFR ) and the slow ( sNGFR ) NGFRs , respectively . ^^^ These observations suggest that the trk protooncogene product , expressed in the absence of the fNGFR , binds NGF with properties characteristic of the sNGFR , which was identified as the high affinity NGFR on primary neurons and PC 12 cells . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Additionally , the inhibition of NGF binding to NGF receptor by MAb is fully consistent with a purely competitive model but is not consistent with a model allowing the formation of a ternary complex of NGF , MAb , and NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In addition to the neurons known to be NGF dependent or containing NGF binding sites , a moderate to strong NGFr immunoreactivity was observed in several non nervous tissues such as the ependymal cells , the epithelial cells of the choroid plexus , the leptomeninge and the cerebral blood vessels . ^^^ The present results provide direct evidence for the presence of non neuronal NGFr in the brain of adult rats , and suggest a possible involvement of NGF in roles other than those classically thought . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Spectroscopic and chemical studies of the interaction between nerve growth factor ( NGF ) and the extracellular domain of the low affinity NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We have characterized the effects of NGF on human B cell proliferation and the regulation of NGF receptor expression on these cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Targeted mutation of the gene encoding the low affinity NGF receptor p 75 leads to deficits in the peripheral sensory nervous system . ^^^ We have generated mice carrying a mutation of the gene encoding the low affinity NGF receptor p75NGFR by targeted mutation in embryonic stem cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NGF stimulated tyrosine phosphorylation of the pp140c trk NGF receptor and tyrosine phosphorylation of pp70trk are also inhibited by similar concentrations of staurosporine and K252A , whereas tyrosine phosphorylation of the EGF receptor , insulin receptor , and 5 src is not affected . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The product of the proto oncogene trk , p140trk , is a tyrosine kinase receptor that has been identified as a signal transducing receptor for NGF , while the role of the low affinity NGF receptor , p75NGFR , in signal transduction is less clear . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Present data demonstrate that the choroid plexus of the rat contain NGFr , probably low affinity NGFr , and suggest an involvement of NGF in the regulation of cerebrospinal fluid secretion , but the importance of these findings , if any , must be investigated in future studies . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Nerve growth factor ( NGF ) promotes the outgrowth of neurites from cultured adrenal chromaffin cells from adult rhesus monkeys , but little is known about the distribution , at the cellular level , of the NGF receptors ( NGFR ) responsible for this response . ^^^ We examined changes in immunostaining for NGFR in chromaffin cells cultured for 4 weeks in the presence or absence of NGF , with or without dexamethasone ( DEX ) , which inhibits neuritic outgrowth from these cells . ^^^ Thus , GCs do not appear to antagonize the effects of NGF merely by decreasing the total number of immunoreactive NGFR on the surface of these cells . . ^^^ Effects of NGF and glucocorticoid on NGF receptor immunolabeling of cultured rhesus adrenal chromaffin cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Tyrosine kinase receptor ( trk ) mRNA encoding an essential component of the high affinity NGF receptor was also present at all stages . ^^^ In contrast , expression of low affinity NGF receptor mRNA was only found in stages VIIcd and 8 of the cycle , the sites of onset of meiosis . ^^^ The low affinity NGF receptor protein was present in the plasma membrane of the apical Sertoli cell processes as well as in the basal plasma membrane of these cells at stages VIIcd to 11 . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Nerve growth factor ( NGF ) binds to a specific cell surface receptor ( NGFR ) that exists in high affinity ( now called trk ) and low affinity ( now called p75NGFR ) forms . ^^^ Differential activation of NGF receptor and early response genes in neural crest derived cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
These changes in CNTF expression were qualitatively distinct from changes in the expression of the low affinity NGF receptor ( p75NGFR ) , which increases following crush . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
More importantly , administration of dexamethasone appears to block the NGF mediated induction of NGFR when both agents are administered simultaneously . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Specific inhibition of NGF receptor tyrosine kinase activity by K 252a . ^^^ Tyrosine kinase activity of gp140trk , a constituent of NGF receptor , is activated by NGF for much a longer period compared to the activation of EGF receptor autokinase activity by EGF . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Immunohistochemical localization of nerve growth factor ( NGF ) and NGF receptor ( NGF R ) in the developing first molar tooth of the rat . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In these cells , which express p75NGFR as only NGF receptor , a time and temperature dependent appearance of a nondisplaceable , trypsin resistant , acid wash stable ligand fraction , followed by an increase of trichloroacetic acid soluble radiolabel in the medium was observed . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Nerve growth factor ( NGF ) is required for the maturation and survival of sympathetic neurons , but the mechanisms controlling expression of the NGF receptor in developing neuroblasts have not been defined . ^^^ MAH cells , an immortalized sympathoadrenal progenitor cell line , did not respond to NGF and expressed neither low affinity NGF receptor ( p 75 ) nor p140trk messenger RNAs . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Our results suggest the presence of NGF binding sites in sensory and sympathetic nerve fibers , and / or their target tissues localized on the skeletal muscles of the rat , whereas the motor nerve fibers lack of NGFr . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The aim of this study was to ascertain whether ALCAR could affect the expression of an NGF receptor ( p75NGFR ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The affinity of hNGF toward human NGF receptor is greater than that of mNGF , but the affinity of mNGF toward rat NGF receptor is greater than that of hNGF . ^^^ Together , these results indicate that the sites on hNGF and mNGF , responsible for binding to NGF receptor , are similar but not identical . ^^^ The data are consistent with a model requiring a low level threshold occupancy of NGF receptor ( Kd = 10 ( 9 ) M ) in order to stimulate full biological activity . ^^^ Together , the data provide insight into regions of the NGF molecule involved in contacts with the receptor leading to formation of the NGF : NGF receptor complex . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Exposure to RA leads neither to an increase in the expression of mRNA for trk , another component of the NGF receptor , nor to an increase in binding to high affinity receptors , suggesting that an increase in the expression of p75NGFR is sufficient to make cells more sensitive to NGF . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Histochemical studies conducted in human spleen and lymph nodes have suggested the presence of NGF receptor ( NGF R ) immunoreactive elements in secondary follicles ; however the nature of the cells bearing the NGF R in lymphoid tissue has not been determined . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Nerve growth factor ( NGF ) mediated up regulation of low affinity NGF receptor gene expression in cultured basal forebrain cholinergic neurons from postnatal 3 day old rats . ^^^ We examined the effect of nerve growth factor ( NGF ) on the expression of low affinity NGF receptor ( LNGFR ) in cultured P 3 basal forebrain cholinergic neurons , on which NGF acts to promote differentiation . ^^^ The increase in LNGFR mRNA was found even at 1 ng / ml NGF ( = 40 pM ) , indicating that the up regulation of the LNGFR mRNA level in cultured cholinergic neurons occurred at an NGF concentration near the Kd value for the high affinity NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
A concomitant depletion of mRNA encoding the 75 Kd NGF receptor ( NGFR ) was observed in those fibroblasts . ^^^ Both NGF and NGFR mRNA remained low thereafter in injured pulp . ^^^ The inverse shifts in fibroblastic mRNA encoding NGF and NGFR were not affected by prior denervation of the tissue , or by pretreatment with dexamethasone . ^^^ Altered expression of NGF and P 75 NGF receptor by fibroblasts of injured teeth precedes sensory nerve sprouting . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Nerve growth factor receptor ( NGFR ) like immunoreactivity ( IR ) was studied in PC 12 cells treated for 96 hr with NGF ( 40 ng / ml ) , using immunogold labeling and electron microscopic morphometric analysis . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The synthesis of nerve growth factor ( NGF ) and nerve growth factor receptor ( NGFR ) were studied in a C 6 glioma cell line by Northern blot hybridization . ^^^ NGF receptor ( NGFR ) mRNA showed changes in expression which were similar to those for NGF mRNA , but were less marked . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Specificity of 192 IgG saporin for NGF receptor positive cholinergic basal forebrain neurons in the rat . ^^^ The data provide further evidence of the selectivity of 192 IgG saporin in abolishing cholinergic , NGF receptor positive CNS neurons . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The grafts consist of a stable population of approximately 2 . 0 10 10 ( 3 ) chromaffin cells that survive for at least 6 months ( longest time point studied ) : Immunoreactivity to catecholamine related enzymes ( tyrosine hydroxylase , dopamine beta hydroxylase ) and the low affinity NGF receptor ( 192 IgG ) are expressed by the chromaffin cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
High and low affinity NGF receptor mRNAs in human foetal spinal cord and ganglia . ^^^ The cellular localization of mRNAs encoding the low affinity NGF receptor ( here referred to as LANR ) and the putative high affinity receptor for NGF , trk , have been studied in the human foetal spinal and sympathetic ganglia , and spinal cord , using in situ hybridization . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Analysis of NGF receptor gene products : generation of artifactual splice variants by PCR . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Ouabain ( 1 10 mM ) decreased histamine release by 25 % supporting the proposed links between NGF receptor mediated events in neurones and the sodium pump . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Therefore , to ascertain whether or not the expression of calcitonin gene related peptide ( CGRP ) , vasoactive intestinal polypeptide ( VIP ) , and galanin was regulated by the retrograde transport of factors such as NGF , we carried out an immunocytochemical analysis using vinblastine as an axonal transport blocker and a monoclonal antibody to the NGF receptor ( NGFR ) as a marker of NGF responsive neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Using partial proteolytic cleavage , the nerve growth factor ( NGF ) binding site and the epitopes for two anti NGF receptor ( NGFR ) monoclonal antibodies were localized on the recombinant extracellular domain ( RED ) of the NGFR . ^^^ Since antibody ME20 . 4 inhibits NGF binding but antibody NGFR 5 does not , these antibodies bind to distinct epitopes . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
At postnatal day 10 NGF receptor immunoreactivity is extended to all glomeruli . ^^^ Double staining methods show that TH and NGF receptor immunoreactivity do not overlap in cell bodies and processes . ^^^ In addition , NGF receptor immunoreactivity is not colocalized with carnosine . ^^^ These findings definitely exclude NGF receptor expression in periglomerular and primary olfactory neurons , suggesting that at least part of NGF receptor expression in the olfactory bulb is associated with glial cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
These changes were similar to those observed for low affinity NGF receptor mRNA and protein , with transection causing increased expression in both Schwann cells and fibroblasts . ^^^ Northern blots confirmed that primary cultured fibroblasts express low affinity NGF receptor mRNA . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Furthermore , it has been shown that an age related loss of NGF receptor ( NGFr ) immunoreactivity occurs in cholinergic septal neurons . ^^^ Quisqualic acid induced lesion of the nucleus basalis of Meynert in young and aging rats : plasticity of surviving NGF receptor positive cholinergic neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Primary demyelination induced by exposure to tellurium alters Schwann cell gene expression : a model for intracellular targeting of NGF receptor . ^^^ Tellurium induced demyelination is associated with downregulation of myelin protein expression and a corresponding upregulation of NGF receptor ( NGF R ) and glial fibrillary acidic protein ( GFAP ) expression . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The second NGF receptor is a member of the trk family of tyrosine kinase receptors , p140trkA . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Thus , although NGF produces the rapid activation of PI 3 kinase through a mechanism that involves tyrosine phosphorylation , there is no evidence for tyrosine phosphorylation of p 85 , or for its ligand dependent association with the NGF receptor . ^^^ Perhaps another phosphoprotein may link the NGF receptor to this enzyme . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
All tissues examined coexpressed the low affinity NGF receptor ( LNGFR ) , whereas none of these tissues expressed the high affinity NGF receptor encoded by the trk protooncogene . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Central or peripheral neurons that express the low affinity NGF receptor are selectively destroyed by the immunotoxin 192 IgG saporin . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In contrast , NGF receptor immunoreactive neurons were only observed in the upper subplate zone of the entorhinal cortex at embryonic week 40 ( term ) , a staining pattern not observed in a 5 year old specimen . ^^^ The expression of NGF receptor immunoreactive neurons within the upper subplate zone between embryonic weeks 16 and 40 was characterized by a dense band of immunoreactive neurons and neuropil . ^^^ NGF receptor immunoreactive neurons were also scattered throughout the lower subplate zone and underlying white matter between embryonic weeks 19 and 26 . ^^^ NGF receptor immunoreactive subplate neurons displayed a topographic distribution with the heaviest concentration found within limbic and paralimbic cortices as well as association neocortex . ^^^ In contrast , light to moderate NGF receptor immunoreactivity was seen in sensory motor cortex . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In contrast , the low affinity NGF receptor , p75NGFR , is expressed in both NGF deprivation resistant and sensitive neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Using a solution hybridization protection assay , levels of low affinity NGF receptor p75NGFR mRNA ( pg / microgram total RNA ) were found to be increased 25 % , with no change in cyclophilin mRNA , in the SCG contralateral to the lesion performed 1 or 3 days earlier . ^^^ The nature of the signals involved in the regulation of NGF receptor mRNA levels and their role in initiating and maintaining collateral sprouting remain to be fully established . ^^^ Nevertheless , the time course of the changes in mRNA levels suggests that regulation of the low affinity NGF receptor gene may be involved in the sequence of events associated with the collateral sprouting response by intact sympathetic nerve cells following partial denervation . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The immunoreactive GAP 43 cells possess elaborate processes that branch from the endplate region into the perisynaptic zone and stain with defined Schwann cell markers : the calcium binding protein S 100 and the low affinity NGF receptor ( NGFr ) , but not with a fibroblast marker , Thy 1 . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Colocalization of NGF binding sites , trk mRNA , and low affinity NGF receptor mRNA in primary sensory neurons : responses to injury and infusion of NGF . ^^^ The distributions of mRNAs for the protooncogene trk and the low affinity NGF receptor ( LNGFR ) were studied by hybridization with oligonucleotide probes on sections of adult rat primary sensory and sympathetic ganglia . ^^^ For comparison with high affinity binding sites , adjacent sections were processed for NGF receptor radioautography . ^^^ Among neurons in lumbar dorsal root ganglia and trigeminal ganglia , trk mRNA and NGF binding sites were closely colocalized ; this finding together with previous direct evidence in other cell types is taken to indicate that trk protein is an essential component of the high affinity NGF receptor in adult sensory neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
At thoracic levels occasional neurons of the intermediolateral column cell group were NGF receptor positive . ^^^ The demonstration of NGF receptor immunoreactivity may provide a useful marker of sensory innervation in the human spinal cord . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
It has been postulated that nerve growth factor ( NGF ) binding to the low affinity fast dissociating NGF receptor ( p 75 NGFR ) on Schwann cells and growing neurites is involved with the molecular feedback necessary for continued neurite extension during development and regeneration . ^^^ Since central projections of somatosensory fibers sprout into the spinal cord after daily neonatal injections of antibodies to NGF ( ANTI NGF ) for a one month period , it is of interest to determine if the distribution of p 75 NGFR correlates with the occurrence of sprouting . ^^^ Thus , ANTI NGF treatment interferes with the postnatal downregulation of p 75 NGFR in the dorsal horn and may provide for continued neurite growth . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Approximately 50 times higher concentrations of ( 10 118 ) rhNGF than ( 1 118 ) rhNGF were required to elicit the same extent of tyrosine phosphorylation of key enzymes in different second messenger pathways , i . e . the NGF receptor tyrosine kinase p140trkA , phospholipase C gamma 1 , and the extracellular signal regulated kinase ERK 1 . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Nerve growth factor ( NGF ) has a modulating effect on immune function , which may occur as a consequence of binding to the NGF receptor ( NGF R ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Purification of embryonic rat motoneurons by panning on a monoclonal antibody to the low affinity NGF receptor . ^^^ Motoneurons are the only neurons within the 15 day embryonic rat spinal cord to express the p 75 low affinity NGF receptor and we show that monoclonal antibody 192 IgG , which binds to the extracellular domain of p 75 , selectively labels a sub population of large multipolar ventral spinal cord neurons in vitro . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Using the monoclonal antibody , ME 20 . 4 , against the p 75 nerve growth factor ( NGF ) receptor , NGF receptor like immunoreactivity has been identified in axonal processes innervating the human hippocampus , where previously a loss of reactivity has been reported in a preliminary study of Alzheimer ' s disease [ 10 ] . ^^^ In an extended analysis of 15 cases of Alzheimer ' s disease , the number of NGF receptor positive fibres in the fimbria and alveus was generally decreased compared with age matched normal groups , in presenile but not senile cases ( differentiated by age of onset before or after 65 years ) . ^^^ This pattern of NGF receptor loss in the hippocampus most closely reflects the loss of basal forebrain cholinergic neurones , previously reported within the different clinical groups but not by biochemical measures of cholinergic function . ^^^ It is concluded that even at moderately advanced stages of Alzheimer ' s disease with onset in the senium , axonal processes and NGF receptor mechanisms may be structurally intact in areas of cholinergic innervation from the basal forebrain , despite evidence of cholinergic dysfunction ( decreased choline acetyltransferase ( ChAT ) and acetylcholinesterase ) , but that in presenile Alzheimer ' s and in demented Parkinson ' s disease cases the receptor declines in conjunction with the loss of subcortical neurones and their processes . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We have isolated mammalian neural crest cells using a monoclonal antibody to the low affinity NGF receptor , and established conditions for the serial propagation of these cells in clonal culture to assess their developmental potential . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
These findings suggest that the perineurial cells function to provide NGF for intact and regenerating axons by binding NGF to NGFR on the perineurial cell surface . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NGF and other growth factors induce an association between ERK 1 and the NGF receptor , gp140prototrk . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Expression of neurotrophins and the low affinity NGF receptor in septal and hippocampal reaggregate cultures : local physiologic effects of NGF synthesized in the septal region . ^^^ Nerve growth factor ( NGF ) , brain derived neurotrophic factor ( BDNF ) and neurotrophin 3 ( NT 3 ) are members of a family of trophic factors designated the neurotrophins , each of which can bind to the low affinity NGF receptor ( LNGFR ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Addition of NGF or an antibody against NGF had no effect on either neuronal survival or the percentage of neurons staining positively for AChE or NGF receptor after 18 20 days in vitro . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
A monoclonal antibody to the low affinity NGF receptor , 192 IgG , coupled to a cytotoxin , saporin , was recently introduced as an efficient selective neurotoxin for the NGFr bearing cholinergic neurones in the rat basal forebrain . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
By utilizing the monoclonal antibody , 192 IgG , which recognizes the p 75 NGF receptor ( NGFR ) , and standard immunohistochemical techniques we have localized p 75 NGFR in variously aged ANTI NGF treated animals and compared the anatomic localization and relative density of the p 75 NGFR immunoreactive ( p 75 NGFR 1 ) regions to same age untreated and preimmune sera treated littermates . ^^^ In addition , we demonstrate that p 75 NGFR 1 levels of ANTI NGF treated rats found in the molecular , the granular and the Purkinje cell layers of the cerebellum , the vestibular nuclei , the spinal tract of 5 and the cochlear nuclei remain at lower concentrations compared to same age control animals . ^^^ We also demonstrate that p 75 NGFR 1 levels in the basal nucleus approaches background levels after ANTI NGF treatment . ^^^ We hypothesize that ANTI NGF biologically inactivates NGF , which over a period of 30 days results in decreased p 75 NGFR 1 . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
A continuing signal maintains NGF receptor expression in hypoglossal motor neurons after crush injury . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In this study we investigated the extent to which the low affinity NGF receptor ( LNGFR ) in C 6 glioma cells can discriminate between the neurotrophins NGF and BDNF . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NGF did not interfere with staurosporine binding , and staurosporine did not affect NGF receptor binding . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Recent data have demonstrated that the product of the tyrosine kinase trk proto oncogene binds NGF and is a component of the high affinity NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
A chimeric human rat NGF receptor transfected into fibroblasts indicates that NGF binding and monoclonal antibody recognition sites are separated but contained within the four cysteine repeats . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Sexually dimorphic expression of the NGF receptor gene in the developing rat brain . ^^^ To define relations between trophic molecules and known sexually dimorphic traits in brain , we examined possible sex differences in nerve growth factor ( NGF ) and NGF receptor ( NGF R ) gene expression in the rat cholinergic basal forebrain ( BF ) hippocampal system . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In this culture study of neurons from adult rat brains , the survival of AChE positive neurons from P 58 rat septum was slightly improved by NGF , although low affinity NGF receptor expression was also observed on cultured P 58 rat septum neurons as well as on those from 2 week old rats . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Investigation of a possible function of NGF in development of intradental innervation has recently begun with the demonstration by others of NGF mRNA and NGF receptor in the pulp . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Recent data indicate that the product of the trk gene , p140trk , is a critical component of the NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Subpopulations of neurons in the septal transplants expressed CAT and the NGF receptor ( 192 IgG ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The predominant type of glial cell resembled Schwann cells and immunostained for the S 100 protein , found in all glial cell types ; glial fibrillary acidic protein ( GFAP ) , found in astrocytes and nonmyelinating Schwann cells ; and showed binding of 217C , a monoclonal Schwann cell marker that binds to the low affinity NGF receptor in glioma cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Like the structurally related proteins nerve growth factor ( NGF ) and brain derived neurotrophic factor ( BDNF ) , NT 3 also binds to the low affinity NGF receptor , a molecule that we suggest to designate low affinity neurotrophin receptor ( LANR ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In PC 12 cells that overexpressed the tyrosine kinase encoded by the trk proto oncogene ( a component of the high affinity NGF receptor ) , the NGF induced activation of the regulatory proteins was potentiated . ^^^ These results suggest that the NGF receptor system enhances the activities of both the guanine nucleotide exchange factor and GAP and that the activation of Ras might be controlled by the balance in activity between these two regulatory proteins . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The 30 month old vehicle infused control rats showed a significant cell loss and cell shrinkage relative to the young control rats in the septal / diagonal band area , the striatum , and the nucleus basalis as assessed by NGF receptor ( NGFr ) and ChAT double label immunocytochemistry . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Terminal differentiation in neuroblastoma cells transfected with the NGF receptor gene when treated with NGF . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Primitive neuroectodermal tumors ( PNETs ) of the CNS and PNS that express functional nerve growth factor receptors ( NGFR ) but fail to differentiate in response to NGF . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Analytical purification of the slow , high affinity NGF receptor : identification of a novel 135 kd polypeptide . ^^^ Although nerve growth factor ( NGF ) action is mediated by the slow , high affinity NGF receptor , little is known regarding its molecular composition or mode of action . ^^^ We have used reversible chemical cross linkers and affinity chromatography strategies to purify the slow NGF receptor covalently cross linked to its NGF ligand . ^^^ The previously characterized 85 kd fast , low affinity NGF receptor is not a component of the cross linked slow receptor ligand complex . ^^^ This newly identified 135 kd polypeptide is either the entire slow NGF receptor , or it might be one component of a larger , multisubunit slow NGF holo receptor . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
These mitogenic effects are absolutely dependent on expression of gp140trk receptors , but do not require the presence of the previously described low affinity NGF receptor . gp140trk also serves as a receptor for the related factor neurotrophin 3 ( NT 3 ) , but not for brain derived neurotrophic factor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
TrkB mediates BDNF / NT 3 dependent survival and proliferation in fibroblasts lacking the low affinity NGF receptor . ^^^ These factors utilize the Trk family of receptor tyrosine kinases , perhaps in conjunction with a second component known as the low affinity NGF receptor ( LNGFR ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Here we show that the antigen recognized by 217c is the rat low affinity NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Three forms of the NGF receptor ( 80 , 83 , and 85 kDa ) displaying a precursor product relationship were detected over the 2 h chase period ; only the 85 kDa species was detected on the cell surface . ^^^ Polymerase chain reaction and RNase protection analyses of NGF receptor mRNA targeted toward the coding region for the transmembrane domain detected no splice variants that could generate truncated receptor , and media conditioned by fibroblasts transfected with rat receptor cDNA , in which splicing can not occur , nonetheless contained the truncated receptor protein . ^^^ Taken together , these results suggest that the truncated NGF receptor does not arise as a distinct translation product but rather from a post translational modification of the intact , surface bound form of the protein . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
An internal deletion in the cytoplasmic tail reverses the apical localization of human NGF receptor in transfected MDCK cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We have recently shown that human epidermal melanocytes , pigment cells derived from the neural crest , express the NGF receptor ( p 75 NGF R ) in vitro ( Peacocke , M . , M . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The distribution of nerve growth factor receptor ( NGF receptor ) like immunoreactivity in pulps of developing primary and mature permanent cat canine teeth was examined , by use of a monoclonal antibody against NGF receptor detected by fluorescence immunohistochemistry and pre embedding immunocytochemical light and electron microscopy . ^^^ Both primary and permanent pulps contained a vast number of NGF receptor like immunoreactive nerves . ^^^ In addition , many blood vessel walls in immature primary tooth pulps showed NGF receptor like immunoreactivity , in contrast to permanent pulps where blood vessels rarely were NGF receptor immunoreactive . ^^^ Double labelling immunofluorescence experiments revealed that in the permanent pulp a majority of the NGF receptor positive nerves also showed calcitonin gene related peptide ( CGRP ) like immunoreactivity , and many showed substance P like immunoreactivity . ^^^ However , nerve fibers with neuropeptide Y like immunoreactivity lacked NGF receptor like immunoreactivity . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NGF infused for 14 days starting either immediately after a unilateral fimbria fornix transection or after a 2 week delay period elicited similar dose response relationships for the maintenance or restoration of ChAT and NGF receptor positivity and cell body size and for intraseptal ' sprouting ' of the axotomized medial septum neurons . ^^^ Since the striatal response began to be detectable at a similar concentration as that required for the full maintenance or restoration of ChAT and NGF receptor positivity it could be seen as an unwanted side effect . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
On these bases , a study on the effect of NGF on the PC 12 cells was carried out to ascertain whether ALCAR induction of NGFR resulted in an enhancement of NGF action . ^^^ Treatment of PC 12 cells for 6 days with ALCAR ( 10 mM ) stimulated [ 125I ] NGF PC 12 cell uptake , consistent with increased NGFR levels . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The role of nerve growth factor ( NGF ) and its receptor ( NGFR ) in the regulation of cholinergic activity has been studied during the aging process . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NGF receptor was expressed in the malignant schwannomas and benign peripheral nerve sheath tumors , but not in the endoneurium of apparently normal peripheral nerves . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
RA was found to also induce the biologically active , high affinity NGF receptor : high affinity receptors were undetectable on dissociated E 7 sympathetic neurons and were observed in vitro only in RA treated neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Knowledge of the time and distribution of NGFR expression in the chick embryo will facilitate the assessment of specific functions of NGF and NGF like molecules in an embryonic model with easy access for experimental manipulations . ( ABSTRACT TRUNCATED AT 400 WORDS ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The occurrence of NGFR immunoreactivity in the otic epithelial cells from early stages of development suggests that NGFR in the epithelia might provide a means to concentrate NGF on the cells which would become the substratum for growing nerve fibers in development . ^^^ Furthermore , the specific appearance of NGFR immunoreactivity in the apical microvillous membrane of the maturing supporting cells implies that NGF secreted into the endolymph from somewhere in the otic epithelia may exert some , yet to be defined effects on the supporting cells in the maturation of the organ of Corti . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The present study was conducted to determine the temporal pattern of NGF receptor expression in cochlear and vestibular ganglia throughout development , and was conducted in two species , quail and mouse . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To explore the molecular mechanisms of nerve growth factor ( NGF ) action , we have attempted to identify proteins that immunoprecipitate with the NGF receptor . ^^^ An anti NGF receptor antibody was developed that immunoprecipitated the 75 Kd receptor in PC 12 cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The cerebellar neuroblasts in primary cultures from five day old rats bore NGF receptor immunoreactivity , suggesting a potential responsive to NGF . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Messenger RNAs for trk and the low affinity NGF receptor in rat basal forebrain . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The levels of nerve growth factor ( NGF ) , its receptor ( NGFR ) and NGFR mRNA were measured in forebrain of 2 day old postnatal rats . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The effects of treatment with L thyroxine ( T 4 ) , 2 . 5 S nerve growth factor ( NGF ) , monoclonal anti NGF and monoclonal anti NGF receptor antibodies , separately or together , on the two main processes of cerebellar histogenesis , the disappearance of the external granular layer ( egl ) and Purkinje cell ( PC ) morphogenesis , were studied in 10 day old ( 1 day after the last injection ) and 15 day old normal and hypothyroid rats . ^^^ In contrast , anti NGF receptor antibodies mainly delay the disappearance of egl , with minor changes in PC morphogenesis . ^^^ Moreover , the effects of NGF treatment , but not those of T 4 , disappear over the long term . ( 3 ) Thyroid deficiency strongly reduces NGF receptor immunoreactivity . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In particular , the gelatinosus and interstitial subnuclei stain intensely for NGF receptor immunoreactivity and for acetylcholinesterase activity . ^^^ Since abundant NGF receptor immunoreactivity is observed also in the nodose ganglion and in the solitary tract , it was assumed that the gelatinosus and the interstitial subnuclei represent the principal sites of termination of primary visceral afferents . ^^^ Because the distribution of acetylcholinesterase activity in the nucleus of the solitary tract matches that of the NGF receptor immunoreactivity rather than that of the cholinergic acetyltransferase immunoreactivity , a non cholinergic function for acetylcholinesterase may dominate in the solitary tract nucleus of the ferret . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The NGF receptor was well detectable also at 8 weeks and localized both in differentiated olfactory cells and in some basal cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We have examined NGF receptor gene expression in these neurons after NGF administration . ^^^ Northern blot and in situ hybridization analyses demonstrated that NGF given systemically to neonatal rats increased levels of NGF receptor mRNA in sympathetic neurons within the superior cervical ganglion . ^^^ NGF receptor mRNA levels were also increased in L 4 L5 dorsal root ganglia , although this mRNA was not expressed uniformly in sensory neurons of control or NGF treated animals . ^^^ In contrast to developing neurons , systemic NGF did not increase NGF receptor mRNA in nonneuronal cells of the sciatic nerve . ^^^ To determine if NGF regulated NGF receptor gene expression at the transcriptional level , we examined PC 12 cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The fate of the genetically modified PC 12 cells ( PC12N . 8 ) was assessed at varying times postimplantation by studying immunoreactivity ( IR ) to tyrosine hydroxylase ( TH ) or the rat NGF receptor ( NGFR ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NCAM and N cadherin directly induced a transcription independent change in the morphology of PC 12 cells from an adrenal to neuronal phenotype and also specifically increased Thy 1 , but not L1 / NILE or low affinity NGF receptor , immunoreactivity . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Ethanol did not increase NGF receptor binding or internalization of NGF . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The pattern of hippocampal NGF receptor immunoreactivity was broadly similar to acetylcholinesterase histochemical localization , indicating a principal localization on cholinergic axons innervating this area . ^^^ Preliminary observations indicate an overall reduction in NGF receptor immunoreactive axons and terminals in old age and Alzheimer ' s disease . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The mean dissociation constant ( Kd ) and the mean capacity ( Bmax ) of the NGF receptor were not significantly different between the 5 AD and 5 normal basal forebrain samples examined . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Rat NGF receptor is recognized by the tumor associated antigen monoclonal antibody 217c . ^^^ Both the quantitative data and the electrophoretic mobility of the immunoprecipitated 125I NGF / receptor complex indicate that the antigen recognized by the 217c mAb monoclonal antibody is , in fact , the NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Here we demonstrate that all three of these neuronal survival molecules bind similarly to the low affinity NGF receptor , but that BDNF and NT 3 , unlike NGF , do not act via the high affinity NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To obtain insight into the site and stage specificity of nerve growth factor ( NGF ) , brain derived neurotrophic factor ( BDNF ) , and neurotrophin 3 ( NT 3 ) action in vivo , we compared the expression patterns of the genes for these three related neurotrophic factors as well as for the NGF receptor in developing and adult rats . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Two distinct NGF receptor complexes are detected on PC 12 cells ; these correspond to 100 kd and 158 kd for the low affinity ( LNGFR ) and the high affinity ( HNGFR ) receptors , respectively . ^^^ Interestingly , three different antibodies directed against distinct epitopes on the LNGFR immunoprecipitate the low but not the high affinity NGF receptor complex . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
This result suggests the possibility that the NGF dependent increase in phosphoinositide hydrolysis in PC 12 cells is due to selective phosphorylation of PLC gamma by serine and tyrosine protein kinases associated with the NGF receptor . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The effects of cAMP on differentiation of cultured Schwann cells : progression from an early phenotype ( 04+ ) to a myelin phenotype ( P0+ , GFAP , N CAM , NGF receptor ) depends on growth inhibition . ^^^ This phenotype included upregulation of the major myelin protein P 0 and downregulation of GFAP , N CAM , A5E3 , and NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Whereas CD 40 is structurally similar to the NGF receptor and is present on all resting B cells , CD 43 displays no homology to other known proteins and is expressed only on a subpopulation of these cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Based on several criteria , this activity has been correlated with the high affinity and not the low affinity NGF receptor complex . ^^^ These observations suggest that the high affinity NGF receptor complex is a substrate of tyrosine kinase activity . ^^^ This observation suggests that this phosphopeptide may represent the phosphorylation of the receptor kinase or the phosphorylation of a coimmunoprecipitating substrate , and possible signal transducing molecule , of the high affinity NGF receptor complex . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Immunohistochemical detection of a monoclonal antibody directed against the NGF receptor in basal forebrain neurons following intraventricular injection . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Presence of a cholinergic projection from ventral striatum to amygdala that is not immunoreactive for NGF receptor . ^^^ By combining the retrograde axonal transport of a fluorescent dye with nerve growth factor ( NGF ) receptor or choline acetyltransferase ( ChAT ) immunocytochemistry , we show that the cholinergic neurons that project most strongly to the basolateral nucleus of the amygdala in the ferret do not possess NGF receptor immunoreactivity in their soma and are situated in the ventral striatum , an area known to receive a massive reciprocal projection from the basolateral nucleus of the amygdala . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Here we show that these phosphorylation events are directly inhibited in PC 12 cells by K252a in a dose dependent manner , indicating that the site of action of this inhibitor is at the NGF receptor level . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Furthermore , PC 12 mutants lacking the Trk NGF receptor tyrosine kinase also lack NGF inducible p21ras activation . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Nerve growth factor ( NGF ) and its receptor ( NGFR ) are proteins that have a role in the normal development and survival of neurons in the peripheral and central nervous systems . ^^^ During development , NGF is necessary for outgrowth of axons and establishment of synapses , and NGFR is the transmembrane protein that binds NGF and brings it into the cell . ^^^ NGF and NGFR expression in the rat olfactory system have been studied previously , and age differences in NGFR are explored further in this study , using immunocytochemistry and immunoelectron microscopy to determine the changes in two different ages : postnatal day 5 and the adult . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Light and electron microscopic immunohistochemical studies on nerve growth factor ( NGF ) receptors in acoustic neurinoma cases were made using a monoclonal antibody to human NGF receptor . ^^^ Immunoreactivity of the NGF receptor was found exclusively on the cytoplasmic membrane of schwannoma cells . ^^^ These findings suggest that NGF receptor expression has some relationship with the formation of interdigitated schwannoma cell processes . ^^^ Other possibilities for the functional significance of the schwannoma NGF receptor are also discussed . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Mutant NGF receptor DNA constructs were expressed in mouse fibroblasts or COS cells , and the ability of the mutant receptor to bind NGF was assayed . ^^^ These results show that the four cysteine rich sequences of the NGF receptor contain the NGF binding domain . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Because the trophic effect of NGF involves a receptor coupling event , the characterization and cellular localization of the NGF receptor ( NGFR ) in the retina are essential to understanding the possible specific action of NGF in this district of the central nervous system . ^^^ Expression of NGF receptor and NGF receptor mRNA in the developing and adult rat retina . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The levels of NGF and NGF receptor mRNA , the degree of macrophage recruitment , and the ability of sensory and motor axons to regenerate were measured in C57BL / Ola mice , in which Wallerian degeneration following a nerve lesion is very slow . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In contrast , NGFR ' s in nonresponsive cell lines are diffusely distributed . ( 2 ) Receptors on responsive cell lines are largely nondiffusing while most receptors on nonresponsive cell lines are relatively free to diffuse . ( 3 ) NGF does not greatly alter the distribution or diffusion properties of the NGFR on either nonresponsive or responsive cell lines . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
These results indicate that gp140trk is a functional NGF receptor that mediates at least some of the signal transduction processes initiated by this neurotrophic factor . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Virtually all ( greater than 90 % ) NGF receptor containing neurons co localize with the specific cholinergic marker choline acetyltransferase ( ChAT ) within the nucleus basalis in PD . ^^^ NGF receptor containing neurons were reduced on average by 68 % ( range 38 . 6 87 . 4 % ) in the non demented PD cases and by 88 . 6 % in the demented PD patient . ^^^ Loss of these neurons was heterogeneous across the nucleus basalis subfields with only the anterolateral and posterior Ch 4 subregions demonstrating significant reductions of NGF receptor containing neurons . ^^^ The reduction in NGF receptor containing neurons was accompanied by a decrease of acetylcholinesterase ( AChE ) containing fibers within temporal cortex and in some cases ChAT immunoreactivity in the basolateral amygdaloid nucleus . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The identification of p140prototrk as an NGF receptor indicates that this protein participates in the primary signal transduction mechanism of NGF . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
On the right track to the NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To determine the domains of the low affinity nerve growth factor ( NGF ) receptor required for appropriate signal transduction , a series of hybrid receptors were constructed that consisted of the extracellular ligand binding domain of the human epidermal growth factor ( EGF ) receptor ( EGFR ) fused to the transmembrane and cytoplasmic domains of the human low affinity NGF receptor ( NGFR ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Loss of AChE and NGFr labeling precedes neuronal death of axotomized septal diagonal band neurons : reversal by intraventricular NGF infusion . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
A clustering of positively charged side chains may provide a complementary interaction with the acidic low affinity NGF receptor . ^^^ The structure provides a model for rational design of analogues of NGF and its relatives and for testing the NGF receptor recognition determinants critical for signal transduction . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Since the biological activity of NGF is mediated by the NGF receptor ( NGFR ) , we hypothesized that defects in the NGF / NGFR pathway may play a role in maintenance of the undifferentiated state of neuroblastomas . ^^^ In addition , we examined the function of the NGFR in these lines by analysis of NGF binding kinetics , as well as by the ability of NGF to induce c fos expression and neurite outgrowth . ^^^ NGF binding assays and Scatchard analyses were performed on the four NGFR positive lines . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The findings establish that ( 1 ) axons from intact neurons , as well as axonal membrane fragments , and extracts of these axons can down regulate Schwann cell NGF receptor number , ( 2 ) central , as well as peripheral , axons have the ability to affect Schwann cell NGF receptor expression , ( 3 ) cross species reactivity is evident with this phenomenon , ( 4 ) physical contact is necessary for axons to down regulate Schwann cell NGF receptor , ( 5 ) the response of axons is glial specific , and ( 6 ) soluble , truncated NGF receptors elaborated by Schwann cells are also down regulated by axon components . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Segregation of NGF receptor in sensory receptors , nerves and local cells of teeth and periodontium demonstrated by EM immunocytochemistry . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
It is not known what role NGF plays in melanoma development , but nevus and malignant melanoma cells express NGF receptor ( NGF R ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
High affinity NGF binding requires coexpression of the trk proto oncogene and the low affinity NGF receptor . ^^^ Reconstitution experiments by membrane fusion and transient transfection into heterologous cells indicate that high affinity NGF binding requires coexpression and binding to both the low affinity NGF receptor and the tyrosine kinase trk gene product . ^^^ These studies reveal a new growth factor receptor mediated mechanism of cellular differentiation involving trk and the low affinity NGF receptor . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In the present study , we characterized these projection neurons by combining the retrograde cell labeling and histochemical stainings for acetylcholinesterase ( AChE ) activity and NGF receptor , choline acetyltransferase ( ChAT ) and gamma aminobutyric acid ( GABA ) immunoreactivities . ^^^ The surviving microsphere labeled neurons were , for the most part , immunoreactive for NGF receptor in the culture . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The full length NGFR expressed in COS cells bound nerve growth factor ( NGF ) with positive cooperativity , but A 875 NGFr and truncated NGFr in COS cells did not display positive cooperativity . ^^^ The anti human NGFr monoclonal antibody NGFR 5 was characterized and found not to compete with NGF for binding to NGFr . ^^^ Fabs were prepared from NGFR 5 and 192 , an anti rat NGFR monoclonal antibody that was previously shown not to compete with NGF for binding . ^^^ Binding of NGF enhanced lateral diffusion of NGFr in A 875 cells and in PC 12 cells in suspension but did not alter lateral diffusion of NGFr in COS cells or in adherent PC 12 cells . ^^^ NGF had no effect on the diffusing fraction or the distribution of NGFR for any cell line . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The effects of NGF are initiated by binding to cell surface receptors ( NGFR ) . ^^^ However , there are two kinds of NGF binding activities and several reported molecular weights of NGFR . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The role of Schwann cells in peripheral nerve degeneration and regeneration NGF NGF receptor system ] . ^^^ When Schwann cells are released from axonal contact , they express NGF receptor on the surface and also secrete NGF . ^^^ These NGF receptor expression and NGF secretion by Schwann cells also extensively occur in the nerves undergoing active degeneration , and subside again when nerve regeneration is completed . ^^^ Taking account of these evidences , 1 discussed the role of NGF NGF receptor system in the peripheral neuropathies . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The primitive TC cells also express the immunoreactivities of neural cell adhesion molecule ( N CAM ) , neuropeptide Y ( NPY ) , and nerve growth factor ( NGF ) receptor ( and NGF receptor mRNA ) . ^^^ DBH , NPY , and NGF receptor immunoreactivities are expressed by TH immunoreactive transitional cells in the fetal rat gut after TH mRNA is no longer detectable . ( ABSTRACT TRUNCATED AT 400 WORDS ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The KD for the affinity of the pure , recombinant human NGF for NGF receptor in PC 12 membranes is 0 . 20 + / 0 . 05 nM . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The NT 4 protein was shown to interact with the low affinity NGF receptor and elicited neurite outgrowth from explanted dorsal root ganglia with no and lower activity in sympathetic and nodose ganglia , respectively . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
These results demonstrate at least three distinct defects in the NGFR pathway in these tumor lines : 1 ) absence of NGFR mRNA or protein expression , 2 ) expression of only low affinity receptor , and 3 ) inability of high affinity receptor to mediate a response to NGF . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Results obtained with this assay demonstrated that expression of both NGFI A and NGFI B is modulated in the peripheral nervous system during postnatal maturation , but not in a pattern concordant with previously characterized NGF receptor expression . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To analyze the possible influence of nerve growth factor ( NGF ) on neurofilament synthesis in primary sensory neurons , adjacent cryostat sections of lumbar dorsal root ganglia ( DRG ) from adult rats were processed for either NGF receptor radioautography or in situ hybridization with a neurofilament cDNA probe . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
These results suggest that ras proteins are involved in at least two different pathways of signal transduction from the NGF receptor , which can be distinguished by differential sensitivity to p 21 ( Asn 17 ) Ha ras . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The NGF receptor ( NGFR ) mRNA was found in the Sertoli cells of rat testis . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The nucleotide and deduced amino acid sequence of a cDNA clone of the chicken NGF receptor ( NGFR ) is reported and is compared with sequences of mammalian NGF receptors . ^^^ Structure and developmental expression of the chicken NGF receptor . ^^^ The functional significance of wide spread embryonic expression of the NGF receptor is discussed . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
These lines initiate neuronal differentiation in response to basic FGF , but not to NGF , and do not contain NGF receptor mRNA . ^^^ Furthermore , an induction of NGF receptor mRNA can be observed in response to FGF . ^^^ Thus , the development of sympathetic neurons may involve a relay , in which FGF both initiates differentiation and induces the NGF receptor , which in turn controls further maturation and survival . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We have studied the expression of NGF receptor ( NGFR ) mRNA in the mouse trigeminal system at closely staged intervals throughout development . ^^^ These findings suggest that NGFR expression on sensory neurons occurs at a low level prior to target field innervation and is up regulated with this event , and they raise the possibility that NGFRs on mesenchymal cells restrict the distribution of NGF in the target field . . ^^^ Expression of the NGF receptor gene in sensory neurons and their cutaneous targets prior to and during innervation . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Transgenic mice carrying multiple copies of the human NGF receptor gene have been generated . ^^^ These transgenic mice will provide an opportunity to study the elements regulating the NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To characterize the latter population , individual neurons were examined in adjacent sections processed either for GAP 43 hybridization or NGF receptor radioautography . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Results obtained with cells transfected with the low affinity NGF receptor gene indicate that these cells bind BDNF , in addition to NGF , whereas cells before transfection do not . ^^^ These data indicate that the low affinity NGF receptor is also a low affinity BDNF receptor and that whatever is conferring high affinity binding and biological response also considerably reinforces the ability of the low affinity receptor to discriminate between NGF and BDNF . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NGF synthesis and NGF receptor expression in the embryonic mouse trigeminal system . 1 . ^^^ Neither NGF synthesis nor NGF receptor expression are dependent on nerve target contact but appear to occur as part of an intrinsic developmental program in the target field and neurons , respectively . 6 . ^^^ The time course of NGF synthesis and NGF receptor expression indicate that NGF does not play a role in guiding axons to their target fields in development . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Moreover , the protein displaced binding of NGF to its receptor , suggesting that it can interact with the NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We analyzed the kinetics and thermodynamics of 125I nerve growth factor ( 125I NGF ) binding to NGF receptor on PC 12 cells . ^^^ Two possible models were examined : binding to two independent receptors at the cell surface ( i . e . high and low affinity forms of NGF receptor ) and a model for consecutive formation of fast , low affinity binding followed by slow , high affinity binding or internalization . ^^^ Neither of these assays shows any evidence that the slow , high affinity binding step is different from internalization of the 125I NGF receptor complex . ^^^ Based on this analysis , there are only two detectable forms of NGF receptor on PC 12 cells : complexes on the surface of the cells with a binding affinity of 0 . 5 nM at 37 degrees C and complexes internalized by the cells . ^^^ Furthermore , the data are consistent with a model in which NGF receptor is internalized constitutively and independently of occupancy by NGF . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In normal skin and fetal skin NGF receptor was good marker for cutaneous nerve sheath and schwann cells . ^^^ In melanocytic lineage , the NGF receptor was not expressed in fetal , normal and dermal melanocyte and weakly positive in only small part of nevus cell nest . ^^^ The distribution of the receptor suggests that the NGF receptor may be involved in the control of proliferation and malignant transformation in melanocytic lineage . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Regional changes in NGF receptor immunohistochemical labeling in the septum of the aged rat . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In the central nervous system ( CNS ) , the presence of nerve growth factor ( NGF ) and its receptor , NGFR , in cholinergic neurons has been demonstrated . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
A species specific monoclonal antibody directed against the human NGF receptor , which blocks NGF binding , reactivated latent HSV in human , but not rat , sensory neurons . ^^^ In contrast , a monoclonal antibody against the rat NGF receptor , which binds the receptor without blocking NGF action , did not produce reactivation . ^^^ These results indicate that the effects of NGF on HSV latency are mediated via NGF binding to the NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Surprisingly , the 55 kd TNF receptor shows a high degree of sequence homology to the NGF receptor extracellular domain . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Human nerve growth factor ( NGF ) receptor ( NGFR ) cDNA was transfected into a neuroblastoma cell line ( HTLA 230 ) which does not express a functional NGF NGFR signal transduction cascade . ^^^ Short term treatment of stably transfected cells ( 98 3 ) expressing membrane bound NGF receptor molecules resulted in a cell cycle dependent , transient expression of the c fos gene upon treatment with NGF , suggesting the presence of functional high affinity NGFR . ^^^ Our data support the idea that introduction of a constitutively expressed NGFR cDNA into cells with neuronal background results in the assembly of a functional NGF NGFR signal cascade in a permissive extracellular environment . . ^^^ Nerve growth factor ( NGF ) induces neuronal differentiation in neuroblastoma cells transfected with the NGF receptor cDNA . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Furthermore , NGF has been shown to modulate immune responses and NGF receptor expression has been localized to lymphoid organs . ^^^ Infant mice that were exposed to alcohol in utero displayed reduced levels of norepinephrine and beta adrenoceptor density , as well as increased NGF receptor expression in the thymus and spleen , but not the heart . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The distribution of FGF R mRNA differed significantly from that of NGF receptor mRNA ; particularly , no hybridization signal was detected in basal forebrain cholinergic neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Alternating phases of FGF receptor and NGF receptor expression in the developing chicken nervous system . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
BDNF produced a 3 fold increase in the number of cells bearing the NGF receptor , as detected by the monoclonal antibody IgG 192 . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Multiple functions for NGF receptor in developing , aging and injured rat teeth are suggested by epithelial , mesenchymal and neural immunoreactivity . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The human NGF receptor is a 427 amino acid protein with a hydrophobic signal sequence , a 222 amino acid extracellular domain , a single transmembrane domain and a 155 amino acid intracellular domain . ^^^ The NGF receptor and a truncated form lacking the cytoplasmic domain were expressed in a COS cell expression system . ^^^ The extracellular domain of the NGF receptor was also expressed in the same system and detected in the COS cell endoplasmic reticulum and in the culture supernatant . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Nerve growth factor ( NGF ) , proNGF and NGF receptor like immunoreactivity in BB rat retina . ^^^ Immunolocalization of the NGF receptor using a monoclonal antibody ( MC 192 ) revealed positive staining of retinal pigment epithelium and Mller cells but not of ganglion cells . ^^^ The staining intensity and distribution of NGF , proNGF , and NGF receptor like immunoreactivity were similar in retinas of diabetic rats . ^^^ These results suggest that NGF is synthesized endogenously in the retina by both neuronal and non neuronal cell constituents , and that diabetes has no detectable effect on NGF / NGF receptor distribution and possibly on NGF biosynthesis . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
This was further supported by immunocytochemical staining of nodose cell bodies in situ with the monoclonal antibody 192 IgG against the NGF receptor ( 12 ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Although CHP707m is the first central nervous system PNET cell line proven to express NGF receptors , immunohistological survey of tissue sections prepared from human central nervous system PNETs showed that 13 of 35 contained NGF receptor positive tumor cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To generate a human central nervous system neuronal cell line that would respond to NGF , we infected the medulloblastoma cell line D 283 MED with a defective retrovirus carrying the cDNA coding for the human NGFR . ^^^ The resultant cells ( MED NGFR ) expressed abundant low and high affinity NGFRs , and NGF treatment induced a rapid transient increase of c fos mRNA in the NGFR expressing cells but not in the parent line or in cells infected with virus lacking the cDNA insert . ^^^ However , the MED NGFR cells did not internalize the NGFR at high efficiency , nor did they differentiate in response to NGF . ^^^ Three important conclusions emerge from this study : ( 1 ) internalization of NGFRs is not necessary for some early rapid transcriptional effects of NGF ; ( 2 ) an unknown factor ( s ) that cooperates with the cloned NGFR in allowing high affinity NGF binding is found in a primitive central nervous system cell line ; and ( 3 ) NGFRs introduced into and expressed by D 283 MED ( i . e . , MED NGFR ) cells are partially functional but are unable to induce differentiation in these primitive neuron like tumor cells , implying that high efficiency receptor mediated endocytosis of NGF and its receptor may be a necessary step in the cascade of events leading to NGF mediated differentiation . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
A peak of expression during Embryonic Days 5 10 was detected in all regions of the chicken central nervous system , but , shortly after birth , only the cerebellar region displays significant levels of NGF receptor protein . ^^^ The time course of expression confirms the dramatic regulation of the NGF receptor gene during defined embryonic periods . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The procedure involves specific labeling of NGF receptors by covalent crosslinking to 125I NGF with a carbodiimide reagent , and subsequent immunoprecipitation of the detergent extracted 125I NGF receptor complexes with the 20 . 4 IgG . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
HT 168 contained more NGF receptor , while HLA DR appeared only on HT 168 MI cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Triap ' s morphological effects were not inhibited by antibodies directed against NGF or the NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In a previous paper ( Development 105 : 251 , 1989 ) , we have demonstrated that a proportion of morphologically undifferentiated neural crest cells express the Schwann cell markers 217c and NGF receptor , and later , as they acquire the bipolar morphology typical of Schwann cells in culture , express S 100 and laminin . ^^^ However , the number of cells expressing the earliest Schwann cell markers 217c and NGF receptor was not affected by the presence of axons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Previous work had shown that this antibody , designated 192 IgG , does not compete with NGF for binding to the NGF receptor of PC 12 cells , but instead interacts with the receptor to increase NGF binding to PC 12 cells ( Chandler , C . ^^^ In the present study , a solid phase separation assay verified the specific formation of a ternary complex of 192 IgG , the NGF receptor , and NGF : 125I labeled 192 IgG precipitated from solution only when incubated with both solubilized NGF receptor and NGF covalently linked to a solid phase ( Sepharose 4B ) . ^^^ These results establish monoclonal antibody 192 IgG as a specific probe for the rat NGF receptor in vitro and in vivo . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We have developed a method to immunoprecipitate rat nerve growth factor ( NGF ) receptor proteins and have applied the method to detect NGF receptor molecules in the rat brain . ^^^ Further , 192 IgG precipitated two radiolabeled proteins , with the expected sizes ( 80 kDa and 210 kDa ) of noncrosslinked NGF receptor components , from among numerous surface iodinated PC 12 cell proteins . ^^^ These results demonstrate the specific immunoprecipitation of NGF receptor molecules by 192 IgG . ^^^ NGF receptor molecules of the same molecular masses as the peripheral receptor components were consistently detected in all of these regions and in preparations from whole brains . ^^^ Removal of the peripheral sympathetic innervation of the brain did not eliminate these NGF receptor proteins , indicating that the receptor is endogenous to central nervous system tissues . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We report that a unique population of rat basal forebrain magnocellular neurons that project to the cortex and hippocampus are immunoreactive to a monoclonal antibody to the NGF receptor . ^^^ Removal of target contact results in a time dependent loss or shrinkage of cells in the basal forebrain that stain for NGF receptor and acetylcholinesterase , suggesting that under normal conditions , basal forebrain cholinergic neurons utilize NGF for trophic support . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Monoclonal antibody 192 , a well characterized antibody which recognizes only the rat NGF receptor , was labelled with 125I and injected into a lateral ventricle of adult rats . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
These antibodies bind to an antigen present on NGF target cells and not on rat fibroblasts lacking NGF receptor . ^^^ They appear thus to be antiidiotypic antibodies directed against the NGF receptor , developed as a consequence of the long term immunization with NGF . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NGF receptors were immunoprecipitated from the EcoRI digested chromatin of HS 294 melanoma cells , of melanocytes proliferating in the presence of 12 O tetradecanoylphorbol 13 acetate , and of SW 707 colorectal carcinoma cells , using a monoclonal antibody to the Mr 75 , 000 cell surface NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The monoclonal anti rat NGF receptor antibody , but not a control antibody , was also transported . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To determine whether a single NGF binding protein is common to both forms of the receptor , a full length receptor cDNA was introduced in the NR 18 cell line , an NGF receptor deficient variant of the PC 12 pheochromocytoma cell line . ^^^ These findings support the hypothesis that a single binding protein is common to both forms of the NGF receptor and suggest that an additional protein is required to produce the high affinity form of the NGF receptor . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In contrast , the human astrocytes in culture did not have an NGF receptor . ^^^ The rat Schwann cells had an NGF receptor whereas the astrocytes did not . ^^^ The functional significance of this NGF receptor on Schwann cells , as well as the lack of the receptor on astrocytes , is discussed . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The cellular distribution of nerve growth factor ( NGF ) receptor ( NGFR ) immunoreactivity in 3 cholinergic nuclei ( medial septal nucleus , nucleus of the diagonal band and nucleus basalis magnocellularis ) of the aged rat brain was compared to that of young adult animals . ^^^ NGF receptor immunoreactivity in aged rat brain . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
No evidence was obtained for an insufficient synthesis of NGF receptor mRNA in the basal forebrain in Alzheimer ' s disease , where NGF receptor like immunoreactivity was confined to neuronal cell bodies . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Previous studies have established that Schwann cells ( SC ) in culture express an NGF receptor . ^^^ In this study , cultures of fetal human SC were established from fetal nerves and various light microscopic ( LM ) and electron microscopic ( EM ) techniques were used to localize the NGF receptor on the SC . ^^^ Results indicate that NGF receptor is localized to the plasma membrane of the SC . ^^^ Quantitative digital analysis determined that the distal portion of the SC process had high concentrations of NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Expression of NGF and NGF receptor mRNAs in the developing brain : evidence for local delivery and action of NGF . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Scatchard analysis yielded a linear plot , indicating the presence of a single class of NGF receptor . ^^^ The average binding constant ( Kd ) was 8 . 0 nM for OV and 8 . 6 nM for CVG , corresponding to the low affinity ( site 2 ) NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
This pattern of NGF receptor mRNA level was observed in all cranial regions and was further correlated with the appearance and disappearance of cell surface receptors . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
On the other hand , the Ch 4 neurons had high levels of NGF receptor protein , whereas those of Ch 5 Ch6 did not . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
This binding occurs in a specific and saturable fashion to what appear to be low affinity ( type 2 ) NGF receptors ( NGFR ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Colocalization of NGF receptor immunoreactivity and [ 3H ] GABA uptake activity in developing rat septum / diagonal band neurons in vitro . ^^^ Cells that expressed nerve growth factor ( NGF ) receptor immunoreactivity were identified immunocytochemically using MC 192 , a monoclonal antibody against NGF receptor . ^^^ Double labeling experiments combining [ 3H ] GABA uptake with immunostaining for NGF receptor showed that 46 % of the NGF receptor immunoreactive cells accumulated [ 3H ] GABA . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In situ expression of nerve growth factor ( NGF ) receptors in human dermal and plexiform neurofibroma , schwannoma and traumatic neuroma was examined by an immunohistochemical method using a monoclonal anti human NGF receptor antibody . ^^^ Immunoreactivity for the NGF receptor was observed on the principal cells of both neurofibroma and schwannoma . ^^^ Immunostaining by the anti S 100 beta protein antibody in adjacent sections suggested that the vast majority of NGF receptor positive cells were also positive for S 100 beta protein . ^^^ In traumatic neuroma , staining for the NGF receptor was more intense in the perineurium than in the endoneurial cells . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The human melanoma cell line A 875 that also expresses the NGF receptor expresses c jun RNA constitutively , and the level of c jun RNA is not affected significantly by NGF . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Intracellular and consequently nuclear uptake of NGF in intact cells was dependent on the presence of surface NGF receptor , whereas nuclear uptake in a cell free system did not correlate with cell surface receptor expression . ^^^ In the cell free system , nuclear transport was inhibited when NGF receptor was being actively synthesized . ^^^ The data suggest that , in intact cells , NGF transported into the cell via the surface receptors is also bound by the NGF receptor being synthesized in the cytoplasm . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Although it is well known that magnocellular cholinergic basal forebrain neurons are trophically responsive to nerve growth factor ( NGF ) and contain NGF receptors ( NGFr ) , the exact distribution of forebrain NGFr immunoreactive neurons and the degree to which cholinergic neurons are colocalized with them have remained in question . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NGF receptor immunohistochemistry therefore represents a sensitive and reliable procedure to selectively visualize forebrain cholinergic neurons for post mortem analysis . ^^^ However , the intracellular staining intensity was reduced in normal aging , suggesting the tentative conclusion that NGF receptor synthesis may decline in the aged brain . ^^^ In Alzheimer ' s disease , the number of NGF receptor positive cells was decreased . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
A monoclonal antibody raised against the receptor for nerve growth factor ( NGF ) has been used to map the distribution of NGF receptor containing profiles within the human basal forebrain of four male and three female elderly patients without neurologic or psychiatric illness . ^^^ Immunohistochemically processed tissue reveals a continuum of NGF receptor positive neurons located within the medial septum , vertical and horizontal limb nuclei of the diagonal band , and nucleus basalis . ^^^ NGF receptor containing neurons are also found within the bed nucleus of the stria terminalis , the anterior commissure , the internal capsule , and the internal and external medullary laminae of the globus pallidus . ^^^ Virtually all ( greater than 95 % ) NGF receptor containing neurons colocalize with the specific cholinergic marker choline acetyltransferase ( ChAT ) or the nonspecific marker acetylcholinesterase ( AChE ) . ^^^ Conversely , a few cholinergic perikarya are found which are not NGF receptor positive ( and vice versa ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Gene transfer of truncated NGF receptor clones leads to cell surface expression in mouse fibroblasts . ^^^ Transfection of recombinant bacteriophage clones encoding human NGF receptor sequences resulted in cell surface expression in mouse fibroblasts . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NGF receptor immunoreactive neurons within the hippocampal projecting nuclei of the medial septum ( Ch 1 ) and vertical limb of the diagonal band ( Ch 2 ) were minimally affected in AD as compared to control cases . ^^^ In contrast , the Ch 4 region demonstrated a significant loss of NGF receptor immunoreactive neurons in AD that inversely correlated ( 0 . 786 ) with the duration of the disease process . ^^^ All four subregions of Ch 4 were affected in the AD cases with the anterolateral ( 76 . 4 % ) , intermediate ( 62 . 1 % ) and posterior divisions ( 76 . 5 % ) demonstrating the greatest reduction in NGF receptor immunoreactive neurons . ^^^ Nissl counterstained sections failed to reveal magnocellular neurons which were not immunoreactive for the NGF receptor , suggesting that reductions in immunocytochemically stained neurons reflects neuron loss and not the failure of viable neurons to synthesize NGF receptors . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In the olfactory bulb of normal rats , nerve growth factor ( NGF ) receptor ( NGFR ) immunoreactivity was largely confined to the glomerular layer . ^^^ NGF receptor increase in the olfactory bulb of the rat after early odor deprivation . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Loss of NGF receptor immunoreactivity in basal forebrain neurons of aged rats : correlation with spatial memory impairment . ^^^ To test the hypothesis that NGF may play a role in the age related decline of cerebral cholinergic function and loss of cognitive ability , we investigated the possible correlation between the loss of basal forebrain neurons that stain for NGF receptor , and impairment of spatial reference memory performance in aged rats . ^^^ Our results suggest that NGF receptor positive basal forebrain neurons undergo marked cell atrophy and loss of neuropil staining in aged rats exhibiting impaired spatial learning and memory performance . ^^^ Overall , the mean number of NGF receptor positive basal forebrain neurons both in the nucleus of the diagonal band and nucleus basalis correlated with retention of the spatial task ( r = 0 . 84 and r = 0 . 67 , respectively ; P less than 0 . 01 ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NGF receptor immunoreactivity ( NGF RI ) is first visible at embryonic day 13 ( E 13 ) in the ventrolateral telencephalic wall and follows a caudal to rostral gradient in its initial appearance . ^^^ The expression of NGF receptor in the subplate zone at the time afferents arrive and its subsequent disappearance with the specific terminal formation suggest that NGF receptor and concomitant accumulation of NGF in the subplate may act as a temporary target for the early arriving basal forebrain afferents ; ingrowing afferents may then be guided by radially oriented NGF RI fibers to proper synaptic sites . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NGF receptor was scarcely expressed in human melanoma ; 9 of 19 melanomas had positive results , but immunoreactivity was generally restricted to rare cells within the larger tumor cell population . ^^^ Antibodies to NGF receptor may play a role in the identification of benign and malignant soft tissue lesions . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We have used in situ hybridization and immunocytochemistry to map the cellular localization of NGF receptor ( NGF R ) mRNA and protein in the adult rat brain . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NGF receptor gene expression is decreased in the nucleus basalis in Alzheimer ' s disease . ^^^ We have used in situ hybridization to show that NGF receptor ( NGF R ) mRNA positive neurons are lost within all divisions of the nucleus basalis of Meynert ( Ch 4 cell group ) in AD patients as compared to normal aged controls . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Expression of NGF receptor in the rat forebrain detected with in situ hybridization and immunohistochemistry . ^^^ The expression of nerve growth factor ( NGF ) receptor mRNA and NGF receptor protein was examined in the adult rat basal forebrain using in situ hybridization and immunohistochemical techniques . ^^^ NGF receptor mRNA and protein were detected within cells in the medial septum , diagonal band of Broca , and nucleus basalis of Meynert . ^^^ As expected , the distribution of NGF receptor mRNA containing cells correlated nicely with the distribution of NGF receptor immunoreactive cells in each of these areas . ^^^ These data extend previous work which suggests that neurons in these areas express the NGF receptor mRNA and manufacture functional NGF receptors . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We have utilized the delayed NGF treatment protocol to ( 1 ) extend to 3 months the delay time between axotomy and NGF treatment , ( 2 ) define the time course of their recovery , ( 3 ) determine that immunostaining for the ( lower affinity ) NGF receptor ( NGFR ) parallels loss and reversal of the ChAT marker , and ( 4 ) evaluate changes in cholinergic somal size following axotomy and subsequent NGF treatment . ^^^ While NGF treatments starting only 7 days after the fimbria fornix transection ( axotomy ) almost entirely restored the number of both ChAT and NGFR positive medial septum neurons , longer delayed ( 2 3 weeks ) treatment brought about recovery from the baseline of 20 25 % to only about 70 % of the control numbers . ^^^ Nerve growth factor ( NGF ) reverses axotomy induced decreases in choline acetyltransferase , NGF receptor and size of medial septum cholinergic neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We examined in vivo and in vitro expression of nerve growth factor ( NGF ) receptors in 13 dermal and 9 plexiform neurofibromas , 28 schwannomas , and 4 traumatic neuromas with an immunohistochemical method using a monoclonal antihuman NGF receptor antibody ( ME 20 4 ) and anti S 100 beta protein antibody . ^^^ Immunoreactivity for NGF receptor and S 100 beta protein was universally observed on the principal cells of both neurofibroma and schwannoma in vivo . ^^^ Moreover , we examined the NGF receptor and S 100 beta protein immunoreactivity in the culture system of the neurofibroma and schwannoma with an immunofluorescent double staining method . ^^^ In traumatic neuroma , however , a positive staining of NGF receptor was more strongly observed in the perineurium rather than in the endoneurial cells . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Chicken nerve growth factor ( NGF ) receptor cDNAs have been isolated and sequenced in an effort to identify functionally important receptor domains and as an initial step in determining the functions of the NGF receptor in early embryogenesis . ^^^ Transient expression of NGF receptor mRNA has been seen in many regions of the developing CNS . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NGF induction of NGF receptor gene expression and cholinergic neuronal hypertrophy within the basal forebrain of the adult rat . ^^^ Chronic infusion of nerve growth factor ( NGF ) into the forebrain of the adult rat produced increases in NGF receptor ( NGF R ) mRNA hybridization , NGF R immunoreactivity , choline acetyltransferase ( ChAT ) mRNA hybridization , and neuronal hypertrophy , when compared with vehicle infusion or noninfused rat brain . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Catecholaminergic sympathetic and sensory neurons from embryonic ganglia , derived from the neural crest , express both the high and low affinity forms of the NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The expression of nerve growth factor ( NGF ) receptor in adult rat brain was studied by immunohistochemistry with a specific anti rat NGF receptor monoclonal antibody , 192 IgG . ^^^ Intense NGF receptor immunoreactivity ( NGFRI ) was found in structures known to be NGF responsive , including forebrain cholinergic neurons in medial septum , diagonal band of Broca , and basal nucleus of Meynert ; central processes of neural crest derived sensory ganglion neurons and their innervated nucleus also contained such immunoreactivity . ^^^ Biochemical study of the NGF receptor showed a major band of molecular weight of approximately 90 KDa in the area postrema , choroid plexus , median eminence , and medial septum with the relative content consistent with that seen by immunohistochemistry . ^^^ No evidence of a truncated NGF receptor was observed . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To ascertain which types of sensory neurons are potentially responsive to NGF , adjacent cryostat sections of rat dorsal root ganglia were processed either for NGF receptor using radioautography or by one of four histochemical procedures . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
An analysis of nerve growth factor ( NGF ) receptor expression and density in human sural nerve biopsies was performed by immunocytochemistry with a murine monoclonal antibody against the human NGF receptor . ^^^ Although there was enhanced expression of NGF receptor within endoneurium in all patients with clinical neuropathies , expression was highest in nerves with axonal disease , consistent with the proposal that disruption of axon Schwann cell interactions triggers the re expression of the NGF receptor . ^^^ These results with human nerves , together with previous studies with animal models , suggest that NGF and NGF receptor play important roles in the general response to neuronal injury . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NGF receptor reexpression and NGF mediated cholinergic neuronal hypertrophy in the damaged adult neostriatum . ^^^ Adult cholinergic interneurons of the neostriatum are not immunoreactive for monoclonal antibody to NGF receptor , whereas the developing neostriatum is immunoreactive for this same antibody . ^^^ Chronic NGF infusion into the adult neostriatum resulted in reexpression of the NGF receptor such that many cholinergic interneurons became immunoreactive for NGF receptor . ^^^ Additionally , in situ hybridization demonstrated an increase in the number of cells expressing NGF receptor mRNA in the NGF infused striatum . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Septal cholinergic neurons were then visualized using NGF receptor immunohistochemistry , which represents a reliable marker for cholinergic neurons in the septal area . ^^^ The fimbrial transections reduced the number of septal NGF receptor positive cells to a similar extent as in young animals . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
However , studies of NGF receptor sites suggest that effects of NGF may also extend to non cholinergic cell groups . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Since the binding of succinylated WGA to N acetylglucosamine residues of cell surface glycoconjugates is not sufficient to prevent the action of NGF , WGA might act on sialic acid residues of the NGF receptor molecule to effect the inhibition of biological actions of NGF . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In WM 266 4 cells , which do not express NGF receptor , NGF does not affect cell proliferation . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We found that ( 1 ) neither GMF beta nor NGF receptor was detectable in intact sciatic nerves , ( 2 ) all Schwann cells of the distal segment of the transected nerve expressed GMF beta as early as 3 d after axotomy that persisted up to 3 weeks , ( 3 ) axonal regeneration repressed the Schwann cell expression of GMF beta , ( 4 ) isolated Schwann cells derived from rat sciatic and adult human sural nerves developed intracellular GMF beta in culture following an initial lag period , and ( 5 ) the induction of Schwann cell NGF receptor coincided temporally with that of GMF beta in the transected nerve and in culture . ^^^ These results show that the expression of GMF beta in Schwann cells , as is the case with the NGF receptor , is induced by the loss of the normal axon Schwann cell contact . ^^^ We propose that the induction of GMF beta , as well as NGF receptor , in Schwann cells after nerve injury plays a role in axonal regeneration . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In newborn rats the levels of nerve growth factor ( NGF ) mRNA ( mRNANGF ) and NGF receptor mRNA ( mRNA ( rec ) ) in the sciatic nerve were 10 and 120 times higher , respectively , than in adult animals . mRNA ( rec ) levels decreased steadily from birth , approaching adult levels by the third postnatal week , whereas mRNANGF levels decreased only after the first postnatal week , although also reaching adult levels by the third week . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In this study , we have examined the expression of the receptor for NGF ( NGFR ) in human neuronal and nonneuronal cells , both in tissue culture and in vivo . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The expression of NGF receptors on human Schwann cells during development and myelination and in culture was analyzed using a murine monoclonal antibody to human NGF receptor . ^^^ Nonmyelinated femoral nerves from 13 to 14 week fetuses stained strongly for NGF receptor , whereas tissues from later stages of development showed a decrease in the staining intensity . ^^^ In adult nerves , only the perineurium and few endoneurial cells were stained with anti NGF receptor antibody . ^^^ Cultured human fetal Schwann cells were positive for NGF receptor by immunofluorescence irregardless of donor age or length of time in culture . ^^^ The decreased staining of NGF receptor with nerve maturation may reflect a dependence of antigen expression on Schwann cell differentiation and / or neuron Schwann cell interaction . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The structure of the lower affinity NGF receptor , determined by gene transfer and cloning , shows it to be a unique , heavily glycosylated protein . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NGF receptor levels on freshly prepared Schwann cells ( day 0 ) were low but increased dramatically during the first week in culture . ^^^ The monoclonal antibody , 192 IgG , which recognizes the rat NGF receptor , was used as an immunohistochemical tool to verify the presence of NGF receptors on the cultured rat Schwann cells . ^^^ The Schwann cell NGF receptor species migrated slower on the gels , with apparent molecular weights of 95 and 220 kDa . ^^^ These results show that NGF receptor levels on Schwann cells increase with time in culture , and this resembles what is observed in Schwann cells in vivo when adult peripheral nerve is injured . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Indirect immunofluorescence microscopy with a monoclonal antineuronal NGF receptor ( NGFR ) antibody indicated that NGFR antigen was expressed on the surface of Schwann cells but not of endoneurial fibroblasts . ^^^ Studies with 125I NGF confirmed this distribution of NGFR in the cultures and showed that the Schwann cell NGFR had a single NGF binding affinity ( Kd of 1 . 8 10 10 ( 9 ) M ) . 125I NGF binding by the cultured Schwann cells increased with time in vitro , reaching a plateau level on the 4th day , but decreased with increasing age , reaching 40 % of the neonatal value in Schwann cells isolated from 12 day old rats . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The results suggest that the NGF / NGFR may have a role in reorganization of septal fibers following entohinal lesion . . ^^^ NGF receptor immunoreactivity in rat brain : topographic distribution and response to entorhinal ablation . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Of the regions in the central nervous system that were sampled , the dorsal spinal cord and basal forebrain were richest in NGF receptor . ^^^ Cross linking studies followed by electrophoresis showed the NGF receptor in the rat basal forebrain to have an apparent molecular weight of 90 kDa . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Axotomy of sciatic nerve fibers in adult rats induces expression of NGF receptor in the entire population of Schwann cells located distal to the injury ( Taniuchi et al . , 1986b ) . ^^^ In the present study we have used immunocytochemistry , with a monoclonal antibody directed against the rat NGF receptor , to examine axotomized peripheral nerves by light and electron microscopy . ^^^ We have found that ( 1 ) the NGF receptor molecules were localized to the cell surface of Schwann cells forming bands of Bungner ; ( 2 ) axonal regeneration into the distal portion of sciatic nerve coincided temporally and spatially with a decrease in Schwann cell expression of NGF receptor ; ( 3 ) Schwann cell NGF receptor could be induced by axotomy of NGF independent neurons , such as motoneurons and parasympathetic neurons ; and ( 4 ) the presence of axon Schwann cell contact was inversely related to expression of Schwann cell NGF receptor . ^^^ Using biochemical assays we have found that , in striking contrast to peripheral nerves , there was no detectable induction of NGF receptor in the spinal cord and brain after axotomy of NGF receptor bearing fibers . ^^^ We conclude that Wallerian degeneration induces Schwann cells , but not central neuroglia , to produce and position upon their plasmalemmal surface the class 2 NGF receptor molecules . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The predominant form of the NGF receptor on Schwann cells exists as an approximately equal to 80 kDa band , as determined by NaDodSO4 / PAGE . ^^^ We demonstrate that cultured Schwann cells shed a truncated ( 50 kDa ) form of the NGF receptor ( NGF Rt ) into their medium . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Expression of NGF receptor in the developing and adult primate central nervous system . ^^^ Monoclonal antibodies against human NGF receptor have been used for immunocytochemical localization of NGF receptors in the CNS of macaques and baboons at various stages of development . ^^^ However , abundant NGF receptor immunoreactivity is present on a population of neurons in basal forebrain , which , on the basis of appearance and pattern of distribution , probably correspond , at least in part , to magnocellular cholinergic neurons of this region . ^^^ NGF receptor immunoreactivity is present on Mueller glia of neural retina . ^^^ In cerebellum , levels of NGF receptor assayed by affinity crosslinking to radioiodinated NGF , and levels of NGF receptor mRNA assayed by Northern blot analysis decrease dramatically during the last month of fetal life . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Furthermore , RNA blot analysis showed the presence of mRNA for the NGF receptor in mouse testis . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Nerve growth factor ( NGF ) receptors in human sural nerve biopsies were detected immunohistochemically using a monoclonal anti human NGF receptor antibody . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
These findings demonstrate the usefulness of colchicine in revealing accumulation of NGFr in adult CNS neurons where previously not visualized and suggest responsiveness of a non cholinergic neuronal population to nerve growth factor ( NGF ) . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Nerve growth factor ( NGF ) receptor expression was studied in rats between embryonic day 11 ( E 11 ) to postnatal day 10 ( PND 10 ) by using a monoclonal antibody , 192 IgG , that specifically recognizes rat NGF receptor . ^^^ Sympathetic ganglia were lightly stained by 192 IgG for NGF receptor immunoreactivity ( NGFRI ) ( E 11 PND10 ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Axons regulate Schwann cell expression of the major myelin and NGF receptor genes . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Factors controlling the expression of the NGF receptor in PC 12 cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To test the hypothesis that NGF may play a role in the age related degeneration of basal forebrain neurons and decline of cerebral cholinergic function , we have used a monoclonal antibody to the NGF receptor , 192 IgG , to immunocytochemically visualize and compare rat basal forebrain neurons responsive to NGF in aged ( 30 months ) and young adult ( 10 months ) rats . ^^^ In a subpopulation of aged rats , NGF receptor immunoreactive cells in the basal forebrain appear vacuolated and shrunken , and the neuropil staining is markedly reduced . ^^^ While no substantial decline in cell density is apparent in Nissl stained sections , the number of NGF receptor positive cell profiles within the vertical limb of diagonal band nuclei is reduced by an average of 32 % in aged rats . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The NGF receptor protein was rapidly processed in oocytes and displayed extensive glycosylation . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Criteria for NGF receptor expression included protein accumulation and cell surface immunofluorescent staining with a monoclonal antibody directed against the human receptor and induction of the messenger RNA species as determined by blot hybridization studies . ^^^ The NGF receptor is inducible in cultured human melanocytes , and we suggest that NGF may modulate the behavior of this neural crest derived cell in the skin . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
A monoclonal antibody raised against the receptor for nerve growth factor ( NGF ) was used to examine the distribution and morphology of NGF receptor containing neurons within the central nervous system of Cebus apella monkeys . ^^^ In a pattern similar to that of cortical acetylcholinesterase ( AChE ) staining , NGF receptor immunopositive fibers were oriented in a tangential plane within the molecular layer of the cortex and in both a radial and tangential fashion within the cortical granular cell layers . ^^^ In addition to neural innervation , there was an extensive vascular apposition by NGF receptor containing neurites on both large caliber vessels and microcapillaries . ^^^ NGF receptor immunoreactivity was extensively , but not exclusively , colocalized with choline acetyltransferase ( ChAT ) and AChE in the basal forebrain . ^^^ A small population of cholinergic neurons were observed that were not NGF receptor immunoreactive . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In the present study , NGF receptors on the surface of cultured Schwann cells were demonstrated by binding of an anti rat NGF receptor monoclonal antibody or of radioiodinated NGF . ^^^ The cultured Schwann cells contained a polyadenylated RNA species homologous with human melanoma NGF receptor mRNA in sequence and size . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Our studies indicate that the NGF receptor ( NGF R ) gene is expressed by anatomically , functionally and biochemically diverse populations , widely distributed in the rat brain , and is not restricted to the basal forebrain / septal region . ^^^ We detect NGF receptor message in frontal cortex , hippocampus , caudate , cerebellum and olfactory bulb . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The type 1 and type 2 NGF receptor species can be interconverted by agents that can manipulate the position of the receptor in the internalization cycle . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Hybridization probes from the transmembrane region of the chick NGF receptor ( NGF R ) that show high homology with the rat NGF R were used to demonstrate an abundant 4 . 5 kb NGF R mRNA in the chick embryo at E3 . 5 . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Scatchard analysis of cultures grown under these conditions again demonstrated the sole presence of the low affinity form of the NGF receptor ( Kd , 3 . 4 nM ) . ^^^ Embryonic catecholaminergic sympathetic neurons exhibit both high and low affinity forms of the NGF receptor , raising the possibility that the Scatchard analysis may not have been sensitive enough to detect the high affinity form of the receptor on a relatively small population of cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
These experiments identify the Mr 158 , 000 species as the slow NGF receptor complex ( chase stable at 0 degree C ) and the smaller Mr 100 , 000 species as the fast NGF receptor complex ( trypsin sensitive ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The antibody , identified as IgG , was able to immunoprecipitate solubilized NGF receptor in the presence or absence of bound NGF . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
These decreased cellular responses are probably due to an underglycosylation of the NGF receptor , since the effects of tunicamycin are correlated with a decrease in 3H fucose incorporation rather than a general decline in cellular metabolism as measured by viability and protein synthesis . ^^^ These results suggest that carbohydrate side chains are important for the function and / or orientation of the NGF receptor in PC 12 cells and that the rapidly dissociating component of NGF binding may be associated with a minimum concentration of functional receptors per cell required for the full biologic response . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
However , cleavage of sialic acid residues with neuraminidase ( EC 2 . 3 . 1 . 18 ) increased the mobility of the NGF receptor complex in a sodium dodecyl sulfate polyacrylamide gel under reducing conditions . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
This is consistent with the finding that 192 IgG recognizes only the rat NGF receptor . ^^^ Thus the decreased protein synthesis caused by the hybrid toxin correlated with the morphological destruction of the neurons . 192 s s A represents a potentially powerful tool by which to selectively destroy NGF receptor bearing cells in vitro . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The WGA induced association with the cytoskeleton , however , is not specific for the NGF receptor , since greater than 90 % of cell surface glycoprotein receptors for WGA become associated with Triton 10 100 insoluble material at lectin concentrations greater than 33 micrograms / ml . ^^^ Concentrations of WGA which change the Triton 10 100 solubility of membrane glycoproteins are similar to those required to alter the kinetic state of the NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
This binding is characteristic of the `` fast ' ' ( low affinity ) NGF receptor , having a Kd of approximately 1 nM and a Bmax of at least 120 fmol / 10 ( 6 ) cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
A possible relationship is also suggested between regulation of cAMP responses and regulation of NGF responses or NGF receptor affinity . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Gene transfer and molecular cloning of the human NGF receptor . ^^^ Mouse L cell transformants have been generated that stably express the human NGF receptor gene transfer with total human DNA . ^^^ Affinity cross linking , metabolic labeling and immunoprecipitation , and equilibrium binding with 125I labeled NGF revealed that this NGF receptor had the same size and binding characteristics as the receptor from human melanoma cells and rat PC 12 cells . ^^^ The sequences encoding the NGF receptor were molecularly cloned using the human Alu repetitive sequence as a probe . ^^^ A cosmid clone that contained the human NGF receptor gene allowed efficient transfection and expression of the receptor . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Membrane proteins were solubilized , and NGF receptor proteins were immunoprecipitated with the monoclonal antibody 192 IgG . ^^^ Analysis by sodium dodecyl sulfate polyacrylamide gel electrophoresis and autoradiography showed that NGF receptor components of Mr = 80 , 000 and Mr = 210 , 000 were phosphorylated . ^^^ We conclude that binding units of the NGF receptor are phosphorylated constitutively in at least two sites in intact cells and that they can be phosphorylated by FA / GSK 3 in vitro . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The effects of NGF on target cells are mediated by a specific cell surface structure , nerve growth factor receptor ( NGFr ) , which has been identified in human cells as a 75 , 000 mol wt glycoprotein . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Characterization of nerve growth factor binding to cultured neural crest cells : evidence of an early developmental form of the NGF receptor . ^^^ In addition , affinity labeling revealed that the 125I NGF receptor complex had a molecular weight of about 93K , characteristic of the low affinity NGF receptor of PC 12 cells . ^^^ The NGF receptor of cultured neural crest cells was trypsin sensitive , as is typical of the low affinity NGF binding sites . ^^^ Cultured neural crest cells which bear NGF receptors were also shown by light microscopic radioautographic techniques to incorporate [ 3H ] thymidine . 1 suggest , therefore , that cultured neural crest cells which have not terminally differentiated , as judged by morphological criteria and continued proliferation , may express an early developmental form of the NGF receptor . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Surgical transection of sciatic nerve was performed on adult Sprague Dawley rats , and , at various times after the operation , the following tissues were dissected for quantitation of NGF receptor : L 4 and L 5 dorsal root ganglia , sciatic nerve proximal to the transection , sciatic nerve distal to the transection , tibialis anterior muscle , and skin of the dorsum of the foot . ^^^ The NGF receptor content of these samples was determined by labeling receptor molecules with radioiodinated NGF ( 125I NGF ) and then specifically immunoprecipitating the 125I NGF receptor complexes with 192 IgG , a monoclonal antibody directed against the rat NGF receptor . ^^^ We observed a time dependent increase in the amount of radioligand labeled NGF receptor proteins found in the distal segment of transected sciatic nerve ; by 7 days the density of receptor ( crosslinked 125I NGF molecules per mg of homogenate protein ) had increased at least 50 fold . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The induction of c fos gene expression was further studied by utilizing a monoclonal antibody ( mAb 192 ) that is directed against the NGF receptor but does not compete with NGF for binding to the receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Identification of NGF receptor in chromatin of melanoma cells using monoclonal antibody to cell surface NGF receptor . ^^^ A 230 KDa species of Nerve Growth Factor ( NGF ) receptor was immunoprecipitated from EcoRI digested chromatin of melanoma cells using a monoclonal antibody to the 75 KDa cell surface NGF receptor . ^^^ The chromatin NGF receptor was shown to exist tightly bound to DNase 2 sensitive sequences which , upon growth factor binding , became resistant to DNase 2 digestion . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Expression and structure of the human NGF receptor . ^^^ The sequence of the extracellular domain of the NGF receptor predicts a highly ordered structure containing a negatively charged region that may serve as the ligand binding site . ^^^ Transfection of a full length cDNA in mouse fibroblasts results in stable expression of NGF receptors that are recognized by monoclonal antibodies to the human NGF receptor and that bind [ 125I ] NGF . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
These observations are best explained by a precursor product relationship for the two NGF receptor species of sympathetic neurons . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Purification to near homogeneity was achieved by chromatography on wheat germ agglutinin agarose , followed by immunoaffinity chromatography on Sepharose columns coupled with anti NGF receptor monoclonal antibody ( MAb ) . ^^^ Possible sequence homology between the NGF receptor and several other proteins is discussed . ^^^ Using the purified receptor as immunogen , new MAbs to the NGF receptor have been produced . ^^^ The NGF receptor was visualized by immunoperoxidase staining in tissue sections of dorsal root ganglia from monkeys . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NGF receptor expression there followed a characteristic pattern . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The identity of the signal ( s ) is unknown ; candidates include NGF itself or some other `` second messenger . ' ' A possible second messenger is the NGF receptor . ^^^ As a first step in assessing the possible role of NGF receptor in the generation of the NGF dependent signal , and in understanding the economy of NGF receptor synthesis and utilization , we determined whether the NGF receptor is retrogradely transported . ^^^ Using immunohistochemical staining with a monoclonal antibody ( 192 IgG ) against rat NGF receptor , we looked for accumulation of NGF receptor molecules distal ( retrograde transport ) , as well as proximal ( anterograde transport ) , to sites of axonal ligation or transection . ^^^ By 10 12 hr in both the ligated sciatic nerve and the lesioned fimbria fornix , accumulated NGF receptor was detected proximal and distal to the ligation / lesion site . ^^^ In both anatomical sites , accumulation of NGF receptor on the proximal ( anterograde ) side occurred in streams of fine axonal processes , whereas staining on the distal ( retrograde ) side occurred in varicose or granular configurations . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To study tissue specific expression of the NGF receptor gene , we have used sensitive cRNA probes for detection of NGF receptor mRNA . ^^^ Moreover , examination of tissues from neonatal and adult rats reveals a marked reduction in steady state NGF receptor mRNA levels in sensory ganglia . ^^^ Our observations suggest that NGF receptor mRNA expression is developmentally regulated in specific areas of the nervous system in a differential fashion . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
When NGFR 2 activity from the acidic region of the PEGG chromatogram was incubated with NGFR 2 from the basic region of the PEGG chromatogram , there was no change in NGF binding or in the number of apparent NGF receptors . ^^^ However , incubation of these same fractions with a fraction having only NGFR 1 showed an apparent increase in high affinity NGF binding and a decrease in low affinity NGF binding . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The ratio of the slow , high affinity to the fast , low affinity NGF receptor decreased over a 5 h incubation with [ 125I ] NGF in a process which did not involve proteolytic conversion of the slow to the fast receptor . ^^^ In contrast , the cross linked fast NGF receptor remained trypsin sensitive for at least 2 h of incubation , indicative of a constant cell surface localization . ^^^ The internalized [ 125I ] NGF in the cross linked slow NGF receptor was not degraded , indicating that cross linking , by preventing the acid pH induced dissociation of the NGF receptor complex in the endosomes , blocks normal sorting of [ 125I ] NGF to the lysosomes . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Scatchard analysis demonstrated losses of both high and low affinity components of NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The site and timing of nerve growth factor ( NGF ) synthesis and NGF receptor expression have been elucidated , thereby clarifying the role of this neurotrophic factor in development . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
It is postulated that the association of NGF or NGF receptor complexes with cytoskeletal elements is instrumental for the mechanism of action of NGF . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
At 2 4 weeks following transection , animals with grafted submaxillary glands exhibited enhanced survival of MS / VDB neurons , which stained positive for acetylcholinesterase and were immunoreactive for the NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The assay involved crosslinking 125I NGF to receptors followed by immunoprecipitation with a monoclonal antibody to rat NGF receptor . ^^^ The NGF receptor was found in whole embryo homogenates as early as embryonic Day 10 ( E 10 ) ( earliest age examined ) . ^^^ The NGF receptor content in whole embryos per milligram protein increased about 3 fold from E 11 to E 18 and decreased slightly at E 20 . ^^^ The NGF receptor content in sciatic nerve homogenates decreased 23 fold from newborn to adulthood . ^^^ The change of NGF receptor level in hindleg muscle had a profile similar to that seen in sciatic nerve . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The results suggest that the Mr = 143 , 000 affinity labeled protein represents a native NGF receptor component . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
WGA also affects the association of the NGF receptor with the Triton 10 100 cytoskeleton . ^^^ Cytoskeleton association of the NGF receptor by WGA shows similar kinetics as the conversion of rapidly to slowly dissociating receptors . ^^^ This interaction may be involved in the alteration of NGF receptor binding properties produced by this lectin . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Elution of purified receptor ( NGF receptor ) was accomplished with 0 . 15 M NaCl , pH 11 . 0 , containing phosphatidylcholine and octylglucoside . ^^^ Scatchard analysis of equilibrium binding of 125I NGF provided similar Kd values for NGF receptors in soluble extracts of A 875 membranes ( 2 . 2 nM ) and with purified NGF receptor ( 3 . 1 nM ) . ^^^ Examination of NGF receptor after electrophoresis on sodium dodecyl sulfate polyacrylamide gels revealed the presence of two major peptides , of Mr = 85 , 000 and Mr = 200 , 000 . ^^^ Affinity labeling experiments , done with 125I NGF and A 875 cells , soluble extracts of A 875 cell membranes , and purified receptor , show that both of these components of the NGF receptor can be specifically cross linked to 125I NGF . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
This finding is consistent with recent studies in which NGF receptor binding has been found in this and other early brainstem and spinal cord motor neuron populations . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The lectin wheat germ agglutinin ( WGA ) alters the characteristics of NGF receptor interaction . ^^^ These data demonstrate that the WGA induced changes in the NGF receptor interaction reflect important alterations in the ability of the receptor to transmit biological signals , resulting in the abrogation of the biological effects of NGF on these cells . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
These MAbs ( 1 ) showed significantly greater reactivity with a melanoma cell line expressing higher levels of NGF receptor , ( 2 ) inhibited the binding of 125I labeled NGF to its receptor , and ( 3 ) immunoprecipitated both metabolically labeled and 125I labeled NGF affinity labeled receptor . ^^^ The NGF receptor was visualized by immunoperoxidase staining in tissue sections of human nevi , melanomas , neurofibromas , a pheochromocytoma , and peripheral nerves . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The increase in uptake does not appear to be due to an increase in NGF receptor number since the number of binding sites is not greatly increased upon NGF starvation . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Three clones lacked NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The order of addition of NGF and WGA has no effect on the rate of conversion of NGF receptor , and the conversion occurs after both NGF and WGA are present . ^^^ The generation of the trypsin resistant , slowly dissociating state of NGF receptor is consistent with a time and temperature dependent conformational change in NGF receptor which occurs after interaction of both NGF and WGA with the receptor or closely associated structures . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Future studies of cell surface phenomena involved in theophylline induced NGF binding site disappearance may lead to a better understanding of the NGF receptor and its disappearance from certain embryonic cells of neural crest origin during differentiation . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Since a slowly dissociating state of the NGF receptor could be produced by receptor clustering , we sought to examine the effects of artificially clustering NGF receptor complexes by using anti NGF antibodies . ^^^ Anti NGF IgG also changes the proportion of Triton 10 100 insoluble NGF binding from 10 % to 50 % , possibly reflecting an association of the NGF receptor with the cytoskeleton . ^^^ Both WGA and anti NGF IgG produce their effects on NGF binding at 37 and 4 degrees C and protect the 125I NGF receptor complex from protease digestion . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The NGF receptor complex migrates as a broad band in NaDodSO4 / polyacrylamide gel electrophoresis with an apparent Mr of approximately equal to 100 , 000 . ^^^ Inhibition of protein glycosylation by tunicamycin generates smaller 125I NGF receptor complexes . ^^^ A second NGF receptor complex with a Mr of 225 , 000 also is obtained from A 875 cells but only occasionally from PC 12 cells . ^^^ Substantial purification of the Mr 100 , 000 NGF receptor complex was achieved by lectin affinity chromatography on columns of wheat germ agglutinin linked to Affi Gel 15 . ^^^ The specific absorption of NGF receptor complexes to these columns indicates that the receptor is a glycoprotein that contains N acetyl D glucosamine . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
These observations coupled with previous findings suggest that the NGF receptor may be a marker found on cells of neural derivation . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
A comparison of binding properties and structure of NGF receptor on PC 12 pheochromocytoma and A 875 melanoma cells . ^^^ However , a difference between equilibrium binding properties of NGF receptor on PC 12 and A 875 cells was observed . ^^^ Cell components other than the binding unit of the NGF receptor may be responsible for the different properties of receptor . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
At 37 degrees C , the NGF receptor complexes cluster and form immobile visible patches . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The NGF concentration changes that elicit neurite retraction suggest that a primary event in retraction may be increased occupancy of a high affinity NGF receptor on neurites . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
TrkA is the NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Nerve growth factor ( NGF ) , a target derived neurotrophic factor , is thought to signal over this distance by receptor mediated internalization of NGF , followed by retrograde axonal transport of the NGF receptor complex . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The loss of low affinity NGF receptor ( p75NGFr ) immunoreactive neurons differed for the toxins ( AMPA cc > QUIS cc > IBO cc ) , whereas treatment with NGF increased the size of the remaining neurons independently of the used toxin . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
PD 90780 , a non peptide inhibitor of nerve growth factor ' s binding to the P 75 NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The binding of MA alpha 2M to trk , which is a part of high affinity NGF receptor , was studied with PC 12 ( 6 24 ) cells and NIH 3T3 fibroblasts expressing trk ( trk 3T3 ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Furthermore , gene expression of brain derived neurotrophic factor ( BDNF ) was found by in situ hybridization and the expression of NGF receptor was found immunohistochemically in the areas where grafted Purkinje and granule cells developed . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
This result , in combination with recent findings concerning NGF synthesis in injured pulp and NGF receptor localization on pulpal sensory fibers , suggests that small diameter sensory fibers in pulp may be under NGF control , but NPY containing sympathetic efferent fibers are not . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Co localization of nitric oxide synthase and NGF receptor in neurons in the medial septal and diagonal band nuclei of the rat . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The RRA uses mouse NGF ( mNGF ) as iodination material and as standard and a rat pheochromocytoma clonal line of cells ( PC 12 ) as a source of NGF receptor ( KD = 3 . 8 10 10 ( 9 ) M ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The ability of a series of substituted kynurenic acids , thienopyridinonecarboxylic acids , and related compounds to inhibit the binding of nerve growth factor ( NGF ) to the p 75 NGF receptor ( NGFR ) was evaluated in a radioligand binding assay that utilized a biotinylated derivative of the extracellular domain of p 75 NGFR ( p75ext ) fixed to streptavidin coated plastic wells . ^^^ These compounds were also found to inhibit the binding of [ 125I ] NGF to its receptors in membranes from PC 12 cells ( which express p 75 as well as trka receptors for NGF ) and DG 44 CHO cells ( transfected with full length p 75 NGFR ) . ^^^ Kynurenic acid derivatives inhibit the binding of nerve growth factor ( NGF ) to the low affinity p 75 NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
These findings suggest that the M 15 , N 60 , and 27 / 21 antibodies bind to a specific site on the surface of NGF where they competitively inhibit binding to the Trk NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Cells with this phenotype expressed the major myelin glycoprotein P 0 and expression of p 75 NGF receptor , N CAM , GFAP and A5E3 proteins was down regulated . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We have further shown that as Schwann cells grow into this nerve wound site they stain intensely for the low affinity NGF receptor p75NGFR . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Furthermore , human placental tissue contains a significant amount of NGF and expresses a NGF receptor . 3 . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The behavioral , biochemical , histological , and electrophysiological effects of a basal forebrain injection of saporin , a ribosome inactivating protein , coupled to a monoclonal antibody against the low affinity NGF receptor ( 192 IgG ) were investigated in adult rats . ^^^ Within the basal forebrain region , the low affinity NGF receptor is exclusively expressed by cholinergic neurons in the medial septal area , diagonal band , and nucleus basalis magnocellularis ( NBM ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Furthermore , concurrent exposure to estrogen and NGF differentially regulated the expression of the two NGF receptor mRNAs . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Retrograde axonal transport and lesion induced upregulation of the TrkA high affinity NGF receptor . ^^^ In this study we examined the distribution and transport of the TrkA NGF receptor using two anti peptide polyclonal antibodies and a monoclonal antibody , all of which are TrkA specific . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Axonal transport of the trkA high affinity NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To determine whether this treatment affected Schwann cell phenotype , we examined expression of the major myelin protein P 0 , and p 75 NGF receptor , both of which are regulated as a function of axon : Schwann cell interactions . ^^^ Levels of p 75 NGF receptor mRNA were unaffected by the cold block , while p 75 NGF receptor protein levels were increased in the region of the nerve immediately adjacent to the cold block , presumably reflecting protein accumulation as a consequence of the block to fast axonal transport . ^^^ These data therefore suggest that P 0 and p 75 NGF receptor are regulated as a function of two different aspects of Schwann cell : axon communication . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The low affinity NGF receptor ( NGFR ) is present on human B lymphocytes and has been shown to have structural homology with another specific B cell surface molecule , CD 40 . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In the case of purified Schwann cells , treatment with TGF beta 1 increases their proliferation , and it promotes a pre or nonmyelinating Schwann cell phenotype characterized by increased NCAM expression , decreased NGF receptor expression , inhibition of the forskolin mediated induction of the myelin protein P 0 , and induction of the Schwann cell transcription factor suppressed cAMP inducible POU protein . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Furthermore , such tight association of GM 1 with Trk appears to be specific , since a similar association was not observed with other growth factor receptors , such as low affinity NGF receptor ( p75NGR ) and epidermal growth factor receptor ( EGFR ) . ^^^ Thus , these results strongly suggest that GM 1 functions as a specific endogenous activator of NGF receptor function , and these enhanced effects appear to be due , at least in part , to tight association of GM 1 with Trk . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The present study was designed to clarify the in vivo function of trkA as an NGF receptor in mammalian neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Here we report that the PI domain of Shc directly binds to tyrosine 490 on the autophosphorylated NGF receptor . ^^^ In addition , the PI domain is able to efficiently compete for binding of full length Shc proteins to the NGF receptor . ^^^ In PC 12 cells , the Shc SH 2 domain interacts with an unidentified tyrosine phosphorylated protein of 115 kDa but not with the activated NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NGF receptor expression in sensory neurons develops normally in embryos lacking NGF . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Evidence indicates that co expression of the two genes for the p 75 receptor and the Trk NGF receptor can potentially lead to greater responsiveness to NGF , and suggests additional levels of regulation for the family of neurotrophin factors . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
A monoclonal antibody to the human low affinity nerve growth factor ( NGF ) receptor , termed p 75 NGFR because of its molecular weight , demonstrated the presence of this receptor in nerve fibers innervating the ovarian vasculature , interstitial tissue , and developing follicles of the gland . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Intracerebroventricular injection of the toxin 192 IgG saporin ( 4 micrograms ) kills the cholinergic neurons of the basal forebrain bearing the low affinity NGF receptor ( NGFr ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Although multimeric complexes of trkA and p 75 were not detected by affinity crosslinking , immunoprecipitation of cross linked NGF receptor complexes with trk specific antibodies resulted in selective immunoprecipitation of crosslinked p 75 . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
This suggests that RET / PTC could share an intracellular signalling pathway with the NGF receptor . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Reverse transcriptase PCR experiments in the five responding cell lines showed expression of both low affinity NGF receptor ( glycoprotein 75 ) and c trk transcripts on the mRNA level . ^^^ Of the five responding cell lines , only 86 HG 39 , the cell line with the lowest responsiveness , revealed low affinity NGF receptor on the protein level ; the other four cell lines with high responsiveness , including the three lung cancer cell lines , expressed no low affinity NGF receptor as shown by fluorescence activated cell sorter analysis and immunoprecipitation using the ME 20 . 4 antibody . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
However , Org 2766 increased immunoreactivity for p 75 low affinity NGF receptor on Schwann cells and evoked the release of neurotrophic factor ( s ) that synergized with NGF in stimulating neurite outgrowth in rat DRG neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In contrast , trkA mRNA , which encodes the high affinity NGF receptor , was undetectable in either region . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The ovary also expresses some of the neurotrophin receptors , including p 75 NGFR , trkB [ the receptor for NT 4 / 5 and brain derived neurotropic factor ( BDNF ) ] , and trkA ( the NGF receptor ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
An ICV injection of NGF produced prominent but transient labeling of cells that contain the low affinity NGF receptor , whereas an injection of BDNF prominently labeled the ventricular ependyma . ^^^ In situ hybridization studies confirmed that the high affinity BDNF receptor ( TrkB ) was much more widely expressed in neurons than was the high affinity NGF receptor ( TrkA ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Depolarization induced neurite outgrowth in PC 12 cells requires permissive , low level NGF receptor stimulation and activation of calcium / calmodulin dependent protein kinase . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To begin to analyze putative relationships of growth factor responsiveness and growth factor / growth factor receptor expressions , IMR 32 , GIMEN , and LAN 1 cell lines were studied for the presence of bFGF , NGF , FGF receptors ( R ) 1 ( flg ) and FGFR 4 , trk , and low affinity NGF receptor ( p 75 ) mRNAs . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Grafts of NGF CINP cells implanted into the septum of adult rats with complete fimbria fornix lesion blocked over 90 % of the cholinergic cell loss in the medial septum and grafts placed in the intact striatum induced accumulation of low affinity NGF receptor positive fibers around the implantation site . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Deletion of a conserved juxtamembrane sequence ( KFG ) in the Trk NGF receptor resulted in impaired neurite outgrowth , somatic hypertrophy , and induction of c fos , c jun , and TIS 1 immediate early genes . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Morphometric analysis of ChAT IR and low affinity NGF receptor IR cholinergic neurons in the middle portion of the NBM shows a prevention of neuronal shrinkage and extensive neuropil in animals treated with microencapsulated NGF secretor fibroblasts . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
B lymphocytes express at their surface the CD 40 antigen which belongs to the NGF receptor superfamily . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Axotomy of motoneurons leads to upregulation of T alpha 1 alpha tubulin and p 75 NGF receptor mRNAs . ^^^ In situ hybridization and image analysis of retrogradely labeled facial motor neurons indicated that such a cold block induced T alpha 1 alpha tubulin and p 75 NGF receptor mRNAs to the same level as did a corresponding nerve transection . ^^^ These results suggest that neurons sense their status by a constant flow of information carried , circulated , or maintained by fast axonal transport and that the axotomy induced increases in T alpha 1 and p 75 NGF receptor mRNAs in motor neurons are , to a great extent , due to loss of such homeostatic signals . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Treatment with transforming growth factor beta 1 ( TGF beta 1 ) results in a marked and rapid downregulation of the low affinity NGF receptor ( NGFR ) on cultured Schwann cells without induction of P 0 gene expression . ^^^ Modulation of Schwann cell phenotype by TGF beta 1 : inhibition of P 0 mRNA expression and downregulation of the low affinity NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Nerve growth factor ( NGF ) , brain derived neurotrophic factor , neurotrophin 3 ( NT 3 ) , and NT 5 stimulated sphinomyelin hydrolysis with similar kinetics in p75NTR NIH 3T3 cells . ^^^ Although brain derived neurotrophic factor ( 10 ng / ml ) was slightly more potent than NGF at inducing sphingomyelin hydrolysis , NT 3 and NT 5 induced significant hydrolysis ( 30 35 % ) at 0 . 1 to 1 ng / ml in p75NTR NIH 3T3 cells . ^^^ In contrast to cells expressing solely p75NTR , NGF ( 100 ng / ml ) did not induce sphingomyelin hydrolysis in PC 12 cells . ^^^ However , in the presence of NGF , NT 3 was unable to induce sphingomyelin hydrolysis , raising the possibility that Trk was modulating p75NTR dependent sphingomyelin hydrolysis . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In mutant PC 12 nnr 5 cells that lack the Trk NGF receptor , the purine analogue sensitive p 75 associated kinase activity was not inducible by NFG . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We document the presence of RNA message for the low ( LNGFR ) and the high affinity NGF receptor ( NTRK 1 ) by using RT PCR on fresh BM aspirate and on LTBMC . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
These data indicate that tyrosine kinase activity of the pp140c trk NGF receptor is essential for the cellular actions of this growth factor . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
B lymphocytes express at their surface the CD 40 antigen which belongs to the NGF receptor superfamily . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Phosphorylation of threonine , but not of tyrosine residues , was inhibited by 6 thioguanine , which therefore inhibits a serine / threonine kinase associated with NGF receptor rather than the receptor kinase itself . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In parallel with the course of NGF synthesis , the expression of low affinity NGF receptor ( p75NGFr ) decreases when a confluent , differentiated , and fully stratified epithelium is obtained . ^^^ The trkA protooncogene product ( p140trkA ) , a component of the NGF receptor , is not expressed by keratinocytes . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Because of the synergistic effects of DHT and NGF , the regulation of NGF receptor mRNA by DHT was evaluated ; however , no significant induction of either trkA or p 75 mRNA expression by androgen was documented . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The authors studied the effect of cold stress on the low affinity NGF receptor ( p75NGFR ) mRNA levels in the basal forebrain and cerebellum of aged rats chronically treated with ALCAR . 3 . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In contrast , expression of mRNAs encoding choline acetyltransferase , p 75 low affinity NGF receptor , neurotrophin tyrosine kinase receptors Trk and TrkB , and brain derived neurotrophic factor showed neuronal sparing in several other regions of the brain . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Correlation between NGF levels in wound chamber fluid and cytological localization of NGF and NGF receptor in axotomized rat sciatic nerve . ^^^ In this study , we analyzed the NGF level in wound fluid by a sensitive enzyme immunoassay and correlated the NGF level with the cytological localization of NGF , low affinity NGF receptor ( p75NGFR ) , and Trk receptor in the axotomized nerve by immunohistochemistry . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
A significant amelioration of the memory impairment and a significant increase in size and number of low affinity NGF receptor ( p 75 ) positive neurons in the basal forebrain were observed . ^^^ Implantation of NGF producing cells into normal young adult rats resulted in a transient but significant memory impairment and hypertrophy of low affinity NGF receptor positive neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Recombinant hNGF was able to save > 70 % of lesioned cholinergic neurons , as assessed by NGF receptor ( NGFr ) and choline acetyltransferase ( ChAT ) immunohistochemistry , from cell death . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Similar estrogen dependent regulation of NGF receptor mRNAs were also observed in the adult dorsal root ganglia , suggesting that estrogen may regulate NGF sensitivity in adult neurotrophin targets as well . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
These results demonstrate that the interaction of NGF with its receptor on human B cells results in the stimulation of major components of the signaling pathway also initiated by NGF receptor ligation in cells of neuronal origin . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
This group of genes is less closely related structurally to the gene structure of the NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Destruction of the cholinergic basal forebrain using immunotoxin to rat NGF receptor : modeling the cholinergic degeneration of Alzheimer ' s disease . ^^^ In order to selectively destroy these neurons , we developed an immunotoxin using monoclonal antibody ( 192 IgG ) to rat NGF receptor ( p75NGFr ) armed with the ribosome inactivating protein , saporin . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We have examined NGF receptor ( NGFR ) mRNA expression in DRG neurons undergoing sprouting and in dorsal root ganglion ( DRG ) neurons whose sprouting had been prevented through exposure to anti NGF antiserum during the course of the experiment . ^^^ Anti NGF treatment blocks the upregulation of NGF receptor mRNA expression associated with collateral sprouting of rat dorsal root ganglion neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Elements in the 5 ' flanking sequences of the mouse low affinity NGF receptor gene direct appropriate CNS , but not PNS , expression in transgenic mice . ^^^ We have initiated a characterization of the cis acting regulatory elements of the murine low affinity NGF receptor ( p75NGFR ) gene . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NGF mediated neuronal differentiation was observed solely in trkA expressing B 104 derived clones and was characterized by reduced cell growth , activation of NGF regulated genes , and downregulation of the endogenous low affinity NGF receptor gene ( gp75NGFR ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The neurite growth observed in plural cell culture without NGF was only partially inhibited by antibody to NGF receptor ( Ab NGFR ) . ^^^ These results suggest that the neurite inducing potency of coexisting cells is via different sites than the NGF receptor . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The spindle shaped subpopulation typically formed small clusters of cells that were immunostained by antibodies to GFAP , vimentin , and low affinity NGF receptor ( LNGFR ) and demonstrated a low incidence of dye coupling ( 30 % ) , whereas the fiber like cells aligned themselves along their long axes , immunostained for gp140trkA , LNGFR , and anti alpha smooth muscle actin and showed extensive ( 100 % ) and diffuse dye spread to numerous other contiguous fiber like cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To determine if immunohistochemical detection of growth factors and / or their receptors correlates with morphological alterations in proliferative lesions of thyroid , we examined the localization of epidermal growth factor ( EGF ) , transforming growth factor alpha ( TGF alpha ) and their common receptor , EGF receptor ( EGF R ) , insulin like growth factor 1 ( IGF 1 ) , IGF 1 receptor ( IGF R ) and IGF binding proteins ( IGFBP ) 1 , 2 , 3 , and 4 , nerve growth factor ( NGF ) , and its receptor NGF receptor ( NGF R ) , transforming growth factor beta ( TGF beta ) , and basic fibroblast growth factor ( bFGF ) , in normal thyroid tissue and various thyroid tumors . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We have demonstrated the expression of Trk A messenger RNA coding for the high affinity NGF receptor in islet cells and the localization of Trk protein in both alpha and beta islet cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
At E 16 in the control , NGF receptor immunoreactivity was expressed in the intermediate zone , subplate and lower cortical plate , and the mode of expression corresponded to the distribution of thalamocortical afferents . ^^^ At the same stage of the reeler mutant , expression of NGF receptor immunoreactivity was confined to the afferent axons that had grown through the neocortical primordium towards the superplate . ^^^ These findings suggest that the subplate and the superplate , which are composed of neurons generated at the earliest stage , attract growing thalamocortical afferent axons specifically by a chemotropic mechanism through the expression of NGF receptor . ( ABSTRACT TRUNCATED AT 400 WORDS ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We have analyzed the effect of prenatal exposure to alcohol on the binding , internalization and secretion of NGF as well as on the content of the NGF receptor ( NGFr ) in cortical rat astrocytes in primary culture . ^^^ Prenatal exposure to ethanol induces a moderate increase in the number of NGFr on the cell surface as well as an increase in the intracellular pool of both NGF and NGFr which is accompanied by an important reduction in the secretion of this factor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Here we have studied changes in the axonal transport of endogenous NGF , NGF receptor ( NGFR ) , and NGFR saturation ( NGF / NGFR ratio ) in the rat sciatic nerve after 2 months of streptozotocin ( STZ ) induced diabetes mellitus . ^^^ Moreover , the NGFR saturation and amount of retrogradely transported NGF were negatively correlated to the individual glucose concentration in diabetics ( r 2 = 0 . 47 and 0 . 55 , respectively ; P < 0 . 0001 ) . ^^^ These findings indicate that , while NGFR expression is normal in the STZ diabetic neuropathy model , the marked decrease in receptor saturation observed in diabetics may reflect low peripheral NGF levels , which in consequence leads to the apparent deprivation of neuronal NGF in diabetic rats . . ^^^ Axonal transport of endogenous nerve growth factor ( NGF ) and NGF receptor in experimental diabetic neuropathy . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Another mAb , 192 IgG , directed against the low affinity NGF receptor , also promotes the adhesion of S 16 , PC 12 , and primary Schwann cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
This down regulation does not occur when PC12nnr5 cells , which lack the high affinity NGF receptor , p140trk , are treated with NGF . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Given the recent findings that the SM cycle is activated to increase the intracellular ceramide level on NGF binding to the low affinity NGF receptor ( LNGFR ) and that NGF binding to LNGFR suppresses apoptosis in neuronal cell lines , our results suggest the possibility of the SM cycle as a signaling mechanism transducing the PCD preventing activity of NGF . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The biological activity of nerve growth factor ( NGF ) has been shown to be mediated by the p140trkA receptor tyrosine kinase , while the role of the p 75 NGF receptor ( p75NGFR ) is still unresolved . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
An NGF beta immunoreactive protein has been localized to the stroma of human prostate tissues , and a low affinity NGF receptor ( gp75NGFR ) has been localized to the adjacent epithelia , consistent with the paracrine regulation of prostate growth . ^^^ In this report we have characterized the expression of the signal transducing component of the NGF receptor , the Trk tyrosine receptor kinase , in prostate epithelial cells that bind exogenous NGF beta and an endogenous NGF beta immunoreactive protein in hPS . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
PNT 1 was shown to efficiently block the chemical crosslinking of NGF , BDNF , and NT 3 to their cognate Trk receptors and to the low affintiy NGF receptor expressed on neuronal and nonneuronal cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
It was previously demonstrated that the high affinity NGF receptor , Trk A , which is sufficient for NGF signal transduction , is expressed in different beta cell lines and in normal rat islet cells in primary culture . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The three mutated sites are situated in two beta loops at one end of the NGF molecule , forming a cleft that could specifically interact with high affinity to the signalling NGF receptor gp140trk . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NGF receptor bearing peripheral glial cells , purified from chicken embryo dorsal root ganglia , were found to secrete neurite growth promoting activity ( NGPA ) but no NGF ( detection limit 1 pM ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Our findings indicate that GM 1 promoted survival can be mediated in part by the Trk NGF receptor as well as by TrkB , and potentially by tyrosine kinase receptors for additional neurotrophic growth factors . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Control monkeys that were either unimplanted or received BHK control ( non NGF secreting ) cell implants did not differ from each other and displayed extensive losses of choline acetyltransferase and p 75 NGF receptor ( NGFr ) immunoreactive neurons within the medial septum ( MS ; 53 and 54 % , respectively ) and vertical limb of the diagonal band ( VLDB ; 21 and 30 % , respectively ) ipsilateral to the lesion . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Colocalization studies revealed that between 66 % and 76 % of trk immunoreactive basal forebrain neurons also expressed immunoreactivity for the low affinity p 75 NGF receptor , an excellent marker for cholinergic basal forebrain cells . ^^^ In this experiment , most single labeled basal forebrain neurons contained only trk immunoreactivity , whereas 4 % of basal forebrain neurons expressed only the low affinity p 75 NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
No significant alteration in NGF receptor density or affinity were noted in the remaining cortex of GM 1 treated decorticated rats , suggesting that GM 1 interacts with an alternative facet of NGF signal transduction to potentiate NGF effects on cholinergic markers . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The decline in p33cdk2 and p34cdc2 kinase activity in response to NGF is accelerated in cells that over express the p140trk NGF receptor , suggesting that the timing of the down regulation is dependent on the level of p140trk and the strength of the NGF signal . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In these cells , NGF ( 100 ng / ml ) failed to induce the mRNA encoding for c fos protooncogene and the low affinity NGF receptor p75NGFR , two NGF responsive genes . ^^^ Our findings indicate that C 6 2B glioma cells do not possess high affinity NGF receptor and thus are unresponsive to NGF and that expression of trkA in neuroectoderm derived cells elicits some of the NGF responses characteristic of neuronal cells . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Recent studies from our group demonstrated the presence of a functional NGF receptor ( NGFR ) on human B lymphocytes . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Maximal inhibition of VGF mRNA induction was achieved using K 252a , an inhibitor of the NGF receptor Trk tyrosine kinase , and by mutating both Y 490 ( SHC association site ) and Y 785 ( PLC gamma 1 association site ) of Trk . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Immunohistochemical localization of the high affinity NGF receptor ( gp 140 trkA ) in the adult human dorsal root and sympathetic ganglia and in the nerves and sensory corpuscles supplying digital skin . ^^^ Recently , a protein tyrosine kinase of congruent to 140 kDa molecular weight , encoded by the proto oncogene trkA , has been identified as the high affinity NGF receptor ( gp 140 trkA ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
These melanoma lines express low affinity neurotrophin receptor p75NTR in relation to their brain metastatic potentials but the variants do not express trkA , the gene encoding a high affinity nerve growth factor ( NGF ) tyrosine kinase receptor p140trkA . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The binding of NGF delta 9 / 13 to Sf 21 cells ectopically expressing the TrkA NGF receptor was also abolished . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The detection of relatively high amounts of NGF synthesis in the BF S was supported in studies demonstrating rapid NGF receptor ( Trk ) activation in the basal forebrain by exogenous NGF and in experiments showing that NGF mRNA was inducible in the BF S by 1 , 25 dihydroxyvitamin D 3 . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Induction of L 1 mRNA in PC 12 cells by NGF is modulated by cell cell contact and does not require the high affinity NGF receptor . ^^^ This increase was not blocked by K252a , an inhibitor of the high affinity NGF receptor , although neurite extension was completely inhibited . ^^^ L 1 mRNA levels also increased in NGF treated mutant PC 12 cells ( PC12nnr5 ) that lack the high affinity NGF receptor . ^^^ Together , these findings suggest that NGF regulates L 1 expression by a mechanism that is independent of the high affinity NGF receptor and that this regulation is modulated by cell cell contact but not by cell extracellular matrix interactions . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We now report that melanocyte stimulation with phorbol 12 tetra decanoate 13 acetate ( TPA ) , previously reported to induce p 75 NGF receptor , also induces trk in melanocytes , and TPA effect is further potentiated by the presence of keratinocytes in culture . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The distribution of the NGF immunoreactive band exactly coincides with the distribution of sprouting cholinergic terminals revealed by acetylcholinesterase histochemistry or NGF receptor immunostaining . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NGF and its receptor ( NGFR ) are suggested to play an important role in the neuronal survival and regeneration under pathologic conditions . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In the present study , we used immunocytochemistry to show that the high affinity NGF receptor protein , gp140trk ( trk ) is detectable in embryonic spinal tissue sections and in cord dissociates . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We have analyzed murine CD4+ T cell clones for their ability to express transcripts encoding NGF , low affinity NGF receptor , and trk protooncogene , the signal transducing receptor subunit for NGF . ^^^ NGF and NGF receptor expression does not seem to be a universal property of all activated CD4+ T cells , since Th 1 type clone 9 / 9 did not express any of the transcripts after either stimulation . ^^^ The absence of low affinity NGF receptor mRNA in resting and activated T cells implies that the low affinity NGF receptor is not involved in NGF signal transduction in CD4+ T cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
EXPERIMENTAL DESIGN : A previously characterized monoclonal antibody ( NGFR 5 ) was utilized for immunohistochemical localization of the p 75 NGF receptor ( NGFR ) in alcohol fixed tissue sections of human fetal kidney ( N = 27 , 54 to 105 days gestation ) , normal mature kidney obtained from nephrectomies for neoplasia ( N = 7 ) , and renal biopsies ( N = 54 ) with various glomerulopathies previously characterized for degree of mesangial alpha smooth muscle actin ( alpha SM ) expression . ^^^ CONCLUSIONS : These studies indicate : ( a ) NGF or other neurotrophins that bind NGFR may be important in human kidney development and glomerular response to injury ; ( b ) NGFR is a marker of developing mesangial cells similar to alpha SM and platelet derived growth factor receptor beta ; ( c ) enhanced expression of NGFR , like alpha SM , is a marker of mesangial cell injury or activation , and that their coordinate upregulation in some glomerular disease states appears to recapitulate a normal developmental state ; ( d ) a population of NGFR and platelet derived growth factor beta expressing interstitial cells can be identified in normal kidneys , which suggests potential signaling mechanisms to recruit or activate these cells at sites of tubulointerstitial injury . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In NGF binding studies using anti trkA and anti low affinity NGF receptor ( LNGFR ) immunoglobulin ( Ig ) G , essentially all binding of NGF can be inhibited . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The low affinity nerve growth factor receptor ( NGFR ) p75NGFR induces apoptosis in the absence of nerve growth factor ( NGF ) binding but enhances neural survival when bound by NGF . ^^^ NGF binding to p75NGFR inhibited the toxicity of beta amyloid peptide , whereas NGF binding to TrkA , the high affinity NGFR , enhanced it . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Other agents , such as fetal calf or carp serum , substrate components , or forscolin failed , however , to induce 36K expression . 04 / 6D2 positive oligodendrocytes could be distinguished from fish 6D2 positive Schwann cells derived from cranial nerves by their antigenic phenotype : Schwann cells but not oligodendrocytes exhibited the low affinity NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The potency of binding of all hNGF variants to the low affinity NGF receptor p 75 was largely unaffected indicating distinct structural contributions of the NH 2 terminal region of hNGF to the binding to TrkA versus p75 . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Furthermore , conditioned medium from clones or lines positive for NGF mRNA was capable of inducing p 140 tyrosine kinase autophosphorylation in 3T3 fibroblasts transfected with cDNA encoding for the tyrosine kinase family NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
While Shc is also found bound to the activated NGF receptor , Trk , no complexes were detectable that contained both Trk and Grb 2 or Sos . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Monkeys receiving the fornix transection and control grafts displayed extensive reductions in the number of choline acetyltransferase ( 57 75 % ) and p 75 NGF receptor ( 53 % ) immunoreactive medial septal neurons ipsilateral to the lesion / implant . ^^^ In contrast , monkeys receiving transplants of encapsulated hNGF secreting cells display only a modest loss of choline acetyltransferase ( 0 36 % ) and p 75 NGF receptor ( 7 22 . 4 % ) immunoreactive septal neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Function perturbing antibodies to the p 75 low affinity NGF receptor potentiated the NT 3 responses of both forms of TrkA in the transfected PC12nnr5 cell lines , suggesting that the low affinity NGF receptor suppresses the ability of TrkA to respond to NT 3 . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Clinical response to retinoic acid was noted only in the two children with tumors coexpressing trk protooncogene mRNA , encoding an essential part of the nerve growth factor ( NGF ) high affinity receptor , and low affinity NGF receptor gene ( LNGFR ) mRNA . ^^^ Clinical stage or age , plasma neuropeptide Y , tumor DNA ploidy and N myc amplification did not as accurately predict response to retinoic acid as NGF receptor mRNAs . ^^^ We hypothesize that part of the therapeutic effect of retinoic acid in neuroblastoma in vivo may be exerted via increased NGF receptor expression and NGF sensitivity . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Recent investigations suggest that this protein may also be a component of the high affinity NGF receptor complex . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Developmental changes in nerve growth factor ( NGF ) binding and NGF receptor proteins trkA and p 75 in the facial nerve . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In order to establish precisely which subsets of neurons are capable of responding to neurotrophins ( NTs ) through the low affinity NGF receptor , p 75 was localized in the primate autonomic and somatic sensory nervous systems . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In contrast , we observed little or no change in the NGFR mRNA levels in regenerating neurons , consistent with the observations that NGF does not play a role in this process . ^^^ Expression of NGF receptor and GAP 43 mRNA in DRG neurons during collateral sprouting and regeneration of dorsal cutaneous nerves . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The brain sections were stained against NGF , NGF receptor , and HSP 70 using the avidin biotin peroxidase method . ^^^ The immunoreactivity for NGF receptor was gradually induced from 1 hour after recirculation with the peak at 1 day in the vehicle group , but it was only slightly induced at 8 hours in the 300 mg / kg DOPS plus benserazide group . ^^^ CONCLUSIONS : Treatment with DOPS is protective to the ischemic hippocampal CA 1 cells , and the NGF receptor system may play a role in this protective effect of DOPS . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The effects on anatomy and behavior of a ribosomal inactivating protein ( saporin ) coupled to a monoclonal antibody against the low affinity NGF receptor ( NGFr ) were examined . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
For an NGF inducible IEG product , such growth factor dependent localization of PC 4 is a novel type of regulation in the pathways from the NGF receptor to the adjacent membrane proteins and to the nucleus . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The results in the glomeruli strongly suggest an effect of the immunotoxin on either periglomerular cells or olfactory nerve terminals , whether directly by NGF receptor ( + ) structures or by trans synaptic mechanisms . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Using a differentiated PC 12 neuronal cell model , we have detected an increase in expression of cell surface NGF receptor protein after acute exposure to nicotine in the micromolar range . ^^^ In addition , we have also observed a persistent effect upon NGF receptor expression which lasted even after nicotine ( nanomolar range ) was removed from the tissue culture medium . ^^^ This increase in cell surface NGF receptor protein was blocked in the presence of mecamylamine , indicating that this effect is likely nicotinic receptor mediated . ^^^ These results are consistent with the hypothesis that the lasting and beneficial actions of nicotine previously observed in vivo may involve an indirect effect upon the level of neuronal cell surface NGF receptor expression . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The results demonstrate that , in addition to Grb 2 , CRK participates in signaling from the NGF receptor and that two GNRPs appear to transmit signals from these adaptor molecules to Ras . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The low affinity NGF receptor , p 75 , can collaborate with each of the Trks to potentiate functional responses to the neurotrophins . ^^^ NGF and the other neurotrophins all bind to the low affinity NGF receptor ( LNGFR ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NGF activity is mediated by cell surface bound receptors including the low affinity NGF receptor ( LNGFr ) which is expressed by some peripheral and central neurons and is present on peripheral nerve Schwann cells during development and regeneration . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
For these reasons , we have used immunocytochemistry to study the distribution of NGF and the low affinity NGF receptor ( p75NGFR ) in the touch domes of adult rat skin . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Collectively , these results demonstrate that : ( 1 ) SH SY5Y cells express functional CNTF receptors ; and ( 2 ) the initial signal transduction mechanism activated by the CNTF receptor in SH SY5Y cells is distinct from that activated by the NGF receptor ; however , both may ultimately stimulate the same downstream biochemical messengers to increase cellular metabolism . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
High levels of BDNF transcripts were observed in the sensory epithelia of all vestibular end organs . mRNAs of low affinity NGF receptor , trkB and trkC , but not of trk , were expressed in the spiral and vestibular ganglia . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Similar results were obtained when an antibody to the low affinity NGF receptor was used to identify the cholinergic neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
BDNF administered locally markedly attenuated the lesion induced decrease of ChAT immunoreactivity and activity and enhanced the lesion induced reexpression of low affinity NGF receptor immunoreactivity in adult facial motoneurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Immunocytochemical staining of C 6 cells with antibodies directed against either the low affinity NGF receptor gp75NGFR or trk proto oncogene products demonstrated a heterogeneous cellular distribution of both antigens . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Using in situ hybridization and immunohistochemistry , we now show that the expression of this receptor is selectively concentrated in a subset of neurons where its appearance is closely associated with that of the NGF receptor TRK . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Time course of cholinergic and monoaminergic changes in rat brain after immunolesioning with 192 IgG saporin . 192 IgG saporin , an immunotoxin targeted at the low affinity NGF receptor , was infused into the lateral ventricle of rat brain . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We first examined the basal forebrain tissues for developmental changes in expression of the high affinity NGF receptor ( trkA ) gene . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We determined whether suramin neurotoxicity can be prevented by nerve growth factor ( NGF ) and if this interaction occurs at the level of the NGF receptor . ^^^ Competitive NGF receptor binding studies were done with 125I labeled NGF in the presence or absence of suramin . ^^^ This effect is most likely due to competition between suramin and NGF at the high affinity NGF receptor . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The immunotoxin 192 IgG saporin , produced by coupling the ribosome inactivating protein saporin to the monoclonal 192 IgG antibody against the low affinity p 75 NGF receptor ( NGFr ) , was injected into the cerebral ventricle , septal area , and substantia innominata of adult rats . ^^^ Dopaminergic and noradrenergic cortical afferents remained intact . 192 IgG saporin damaged two neuronal groups outside the basal forebrain that express the p 75 NGF receptor : NGFr positive cerebellar Purkinje cells after intraventricular injection and cholinergic striatal interneurons after injections into the substantia innominata . ^^^ Complete and selective cholinergic denervation of rat neocortex and hippocampus but not amygdala by an immunotoxin against the p 75 NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Survival of the transplanted human SCs was established by dissociating nerve explants taken from the regenerated cable ( after first placing them in culture for 5 d ) and staining individual cells for a primate specific NGF receptor ( PNGFr ) and S 100 . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Depolarizing influences increase low affinity NGF receptor gene expression in cultured Purkinje neurons . ^^^ To begin investigating the molecular mechanisms by which trophic interactions and neural activity modulate cerebellar development , we have now studied the role of excitatory signals on the expression of both NGF and the p 75 glycoprotein ( the low affinity component of the NGF receptor ) by cerebellar cells in culture . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
CGRP immunoreactive neurons in rat dorsal root ganglia do not all contain low affinity NGF receptor immunoreactivity . ^^^ Nerve growth factor ( NGF ) and its homologues bind to the low affinity NGF receptor ( LNGFR ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Approximately 15 % of the neurons in this culture system were immunoreactive for the low affinity NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Expression of the low affinity NGF receptor during human muscle development , regeneration , and in tissue culture . ^^^ The expression of the low affinity NGF receptor ( LNGFR ) during human muscle development , regeneration , and in tissue culture was analyzed using a murine monoclonal antibody to human LNGFR ( MAb ME 20 . 4 ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We have previously shown that nerve growth factor ( NGF ) induces a rapid and relatively continuous activation of Ras in rat pheochromocytoma PC 12 cells while epidermal growth factor ( EGF ) activates Ras transiently , and that tyrosine kinase activity of the NGF receptor is essential for the activation of Ras ( Muroya et al . , Oncogene , 7 , 277 281 , 1992 ) . ^^^ In contrast to Shc , Grb2 / Ash bound to EGF receptor but did not form a stable complex with the NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Expression and functionality of the trkA proto oncogene product / NGF receptor in undifferentiated hematopoietic cells . ^^^ The expression of the low affinity NGF receptor ( p 75 ) and the trkA proto oncogene product was analyzed in a series of human hematopoietic cell lines at protein and RNA levels . ^^^ We did not detect any form of NGF receptor in cell lines displaying a myelomonocytic phenotype ( HL 60 and U 937 ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Our experiments show that ethanol ingestion causes a reduction of NGF in the hippocampus , of choline acetyltransferase activity in both the septum and hippocampus , and in the distribution of NGF receptor in the basal forebrain cholinergic neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Analysis of the trk NGF receptor tyrosine kinase using recombinant fusion proteins . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Our findings that neither mitogen nor Ag stimulation induced the expression of the low affinity NGF receptor in 9 / 6 T cells indicate that trk alone is sufficient to mediate biologic activity of NGF in T lymphocytes . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
These data demonstrate an increase in activation of the high affinity NGF receptor with a compound that elevates levels of NGF in the central nervous system , and they support the strategy of discovering a pharmacological agent that induces NGF as a method for treating neurodegenerative disorders . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Migrating Schwann cells in developing or regenerating peripheral nerves are known to express dramatically increased levels of nerve growth factor ( NGF ) and the low affinity NGF receptor ( LNGFR ) . ^^^ In contrast to neurons , where pp140trk appears to be the functionally critical NGF receptor , NGF responses in Schwann cells depend on LNGFR . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
This study was designed to examine the relationship between cognitive function and endogenous levels of NGF , low affinity NGF receptor ( LNGFR ) , and amyloid precursor protein ( APP ) mRNAs . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NGF promotes neurite outgrowth and proliferation from such cells , indicating that Trk is sufficient to mediate such responses in the absence of significant levels of the endogenous 75 kDa low affinity NGF receptor ( p 75 ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
If this is the case , neurons in the prevertebral ganglia should express another neurotrophin receptor , in addition to the NGF receptor ( trkA ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Furthermore , in peripuberal and adult mouse and rat testis we have identified the expression of an abundant and shorter mRNA of 3 . 2 kb that cross hybridizes to the low affinity NGF receptor transcript ( 3 . 7 kb ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The number of p 75 NGF receptor immunoreactive neurons in the NBM was significantly decreased ( 49 % ) by the lesion and was not affected by NGF or BDNF . ^^^ Similarly , in situ hybridization showed enhanced expression of the p 75 NGF receptor following treatment with NGF , but not with BDNF . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Animals injected with antibodies to NGF or an NGF receptor had reduced ganglion cell numbers and normal brainstem patterns . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
On the other hand , expression of the high affinity NGF receptor ( trkA ) is restricted to cholinergic neurons in basal forebrain and striatum . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NGF like immunoreactivity exclusively colocalized within p 75 NGF receptor ( NGFR ) containing basal forebrain neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The cultures contain a small number of motoneurons as judged by their immunostaining with ChAT , low affinity NGF receptor ( LNGFR ) , and large size . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Levels of the truncated , extracellular portion of the NGF receptor ( NGF Rt ) are elevated in urine of patients with peripheral neuropathies and in animals with peripheral nerve injury , but it has not been determined whether urine levels of NGF Rt are altered by the presence and / or progression of dementia related neuropathologic changes in patients with Alzheimer ' s disease . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The biological responsiveness of neural cells to nerve growth factor ( NGF ) appears to require expression and ligand binding to both the low affinity NGF receptor ( LNGFR ) and the proto oncogene product trk , the latter being a receptor tyrosine kinase . ^^^ Immunolocalization of the LNGFR and the high affinity component of the NGF receptor , trk ( HNGFR ) was studied by electron microscopic morphometric analysis on cultured PC 12 pheochromocytoma cells , C 6 glioma cells and neonatal rat dorsal root ganglia neurons using a double immunogold labeling technique . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Thus , trkA expression in HTLA 230 cells is sufficient to generate a functional NGF receptor complex that leads to growth arrested and differentiated NB cells in vitro and in vivo in the presence of NGF . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The activity of the neurotransmitter synthesizing enzyme choline acetyltransferase ( ChAT ) , the activity of which is regulated by NGF , has also been determined in parallel experiments . p 75 NGF receptor immunoreactivity was detected in the fetal neocortex as nerve terminal staining , potentially derived from basal forebrain neurons which were positive for NGF receptor , and was also localized in nerve cells of the cerebral cortex . ^^^ Cortical reactivity for NGF receptor increased with age up to the 4th decade thereafter remaining constant . ^^^ NGF receptor reactivity localized to neocortical neuronal cell bodies was not present in the postnatal or adult brain . ^^^ Hippocampal reactivity for the NGF receptor was not present before birth appearing first in the postnatal period and thereafter showing an identical development pattern to the neocortex . ^^^ ChAT activity in the entorhinal cortex and hippocampus partially paralleled NGF receptor development being present in the neocortex in the fetus but not in the fetal hippocampal formation and increasing postnatally to reach maximum levels in the 4th decade . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Cold water swimming stress alters NGF and low affinity NGF receptor distribution in developing rat brain . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The concentration of NGF required to activate Fos kinase is consistent with signaling from the high affinity NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In vitro enfolding of olfactory neurites by p 75 NGF receptor positive ensheathing cells from adult rat olfactory bulb . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
There are several different possible ways of stimulating NGF receptors such as NGF infusion , implantation of slow release biodegradable pellets , using carrier mediated transport across the blood brain barrier , grafting NGF producing cells , transferring genes directly to the brain , developing NGF receptor agonists , or controlling the endogenous NGF production . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
These cells were also labeled with a monoclonal antibody to the NGF receptor , 192 IgG , suggesting that low affinity NGF receptors are associated with a nonneuronal population . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Immunohistochemical localization of nerve growth factor ( NGF ) and NGF receptor in the hypothalamus of adult rats . ^^^ The aim of the present study was to improve immunohistochemical methodologies for the investigation of NGF and NGF receptor ( NGF r ) in the hypothalamus of adult rats . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We have investigated the role of trkA , the tyrosine kinase NGF receptor , in mediating the survival response of embryonic neurons to NGF . ^^^ The failure of trkA to result in an NGF promoted survival response in ciliary neurons is not due to absence of the low affinity NGF receptor , p 75 , in these neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We tried to detect NGF and NGF receptor in the olfactory epithelium of young and mature rats with an immunohistochemical technique . ^^^ Immunoreactivity to NGF and NGF receptor was detected in all specimens of olfactory epithelium . ^^^ Immunoreactivity to NGF receptor was positive in the basal cell layer and on fibers piercing the olfactory epithelium . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Colocalization of NGF receptor with VIP in rat suprachiasmatic neurones . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Patterns of nerve growth factor ( NGF ) , proNGF , and p 75 NGF receptor expression in the rat incisor : comparison with expression in the molar . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Receptors for NGF ( NGFR ) have been demonstrated on cholinergic perikarya in the medial septum , diagonal band of Broca , and basal nucleus of Meynert . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In situ hybridization and image analysis were then used to determine neuronal levels of p 75 NGF receptor , trkA , and T alpha 1 alpha tubulin mRNAs , with the latter providing a correlative measure of neuronal sprouting . ^^^ Grain counts for p 75 NGF receptor mRNA increased from 2 to 6 fold in the NGF treated neurons , and grain densities , which accounted for neuronal hypertrophy , also increased significantly . ^^^ The NGF induced increased in p 75 NGF receptor mRNA levels was accompanied by terminal sprouting and by an increase in the density of p 75 NGF receptors on terminal neurites , as indicated by IgG 192 immunostaining of the NGF treated iris . ^^^ These data therefore suggest that , in addition to promoting local sprouting , increased target derived NGF increases the levels of p 75 NGF receptor relative to trkA on terminal neurites , by differentially regulating receptor synthesis . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Immunostaining for the low affinity NGF receptor ( LNGFR ) , intense in the NGF supported neurons , was absent in the CNTF supported neurons and reduced in the neurons exposed to the combination of NGF and CNTF . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Since previous studies have shown that these three neurotrophins bind to the low affinity NGF receptor , p75NGFR , with similar affinity , our data suggest that the retrograde transport of neurotrophins by motoneurons may be mediated by additional components , such as the trk family of proto oncogenes . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In order to determine if the IT was killing NGF receptor positive neurons in the CBF ( rather than simply suppressing the cholinergic phenotype in these cells ) , a population of neurons in the nucleus basalis magnocellularis ( NBM ) was prelabeled by an intracortical injection of the neurotracer Fluoro Gold ( FG ) 1 week before intraventricular injections of IT or control substances ( reduced IT or phosphate buffered saline ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The present study was designed to examine ( 1 ) if neural neurotrophin targets respond to estrogen as a general phenotypic feature and ( 2 ) if NGF receptor mRNAs are regulated by estrogen , using a prototypical target of NGF , the dorsal root ganglion ( DRG ) ( sensory ) neuron . ^^^ In contrast , although the mRNAs for the NGF receptors p75NGFR and trkA were also expressed in DRG neurons from OVX and intact animals , expression of both NGF receptor mRNAs was upregulated in sensory neurons during proestrus , as compared to the OVX condition . ^^^ Estrogen regulation of NGF receptor mRNA in adult peripheral neural targets of the neurotrophins supports the hypothesis that estrogen may regulate neuronal sensitivity to neurotrophins such as NGF and may be an important mediator of neurotrophin actions in normal neural function and following neural trauma . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The low affinity NGF receptor was found in the uninduced mesenchyme at early developmental stages , but in the glomerular podocytes at later developmental stages . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Trk immunoreactivity was observed in basal forebrain and striatal cholinergic neurons , whereas low affinity NGF receptor immunoreactivity was observed in basal forebrain but not striatal cholinergic neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The morphological parameters analyzed showed a high degree of heterogeneity among clones , consisting with a different expression of the high affinity NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Induction of apoptosis by the low affinity NGF receptor . ^^^ In contrast to TrkA , the high affinity NGF receptor that transduces NGF signals for survival and differentiation , the function of the low affinity NGF receptor , p75NGFR , remains uncertain . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The signal transduction cascade triggered by NGF receptor binding may prevent the action of neocarzinostatin or the expression of the cellular changes induced in SK N SH cells by neocarzinostatin . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Immunohistochemical and Northern blot analysis revealed high levels of low affinity NGF receptor protein and mRNA in differentiated RN33B cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Developing rat retinal ganglion cells express the functional NGF receptor p140trkA . ^^^ In in vitro preparations of retinal neurons from neonatal rats the functional NGF receptor p140trkA was immunocytochemically detected on retrogradely labeled retinal ganglion cells ( RGCs ) . ^^^ The bands at 90 and 95 kDa correspond to the so called low affinity NGF receptor p75NGFR . ^^^ The presence of the functional NGF receptor on RGCs provides the molecular explanation for the reported sensitivity of these cells to the biological action of NGF . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In fetal and paediatric normal thymus , EGF receptor was expressed by subcapsular , cortical and medullary epithelial cells , whereas immunoreactivity for NGF receptor was detected on only subcapsular and medullary epithelial cells . ^^^ In contrast , the pattern of expression of NGF receptor in thymomas was different for the various histological types . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
TrkA NGF receptor expression by non cholinergic thalamic neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In addition , the long term effects of these agents on choline acetyltransferase ( ChAT ) , glutamic acid decarboxylase ( GAD ) activity , and choline uptake , as well as on ChAT and NGF receptor ( p75NGFR ) immunoreactivity in various brain regions , were also investigated . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Like NGF , BDNF and NT 3 crosslinked to the low affinity NGF receptor ( p75NGFR ) on PC 12 cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Expression of low affinity NGF receptor and trkB mRNA in human SH SY5Y neuroblastoma cells . ^^^ We demonstrate that SH SY5Y cells express transcripts encoding the low affinity NGF receptor ( LNGFR ) and trkB , the signal transducing receptor unit for BDNF . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The enhancement of chemotactic response and heparanase production was detected at NGF concentrations sufficient to fully saturate both low and high affinity NGF receptors ( NGFR ) , the neurotrophin receptor ( p 75 ) and the trkA gene product , respectively . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The present study was designed to assess whether treatment for 6 months with a daily dose of 100 mg / kg of choline alfoscerate has any effect on the expression of NGF receptor immunoreactivity in male Wistar rats of 24 months of age . ^^^ NGF receptor immunoreactivity which was developed in the 3 layers of the cerebellar cortex in adult rats was decreased in the neuropil of the molecular layer and in the cytoplasm of Purkinje neurons of rats of 24 months . ^^^ The number of NGF receptor immunoreactive Purkinje neurons was also lower in the oldest age group , whereas the NGF receptor immunoreactivity in the cytoplasm of granule neurons was unchanged . ^^^ Treatment with choline alfoscerate increased NGF receptor immunoreactivity in the molecular layer and in the cytoplasm of Purkinje neurons as well as the number of immunoreactive Purkinje neurons but was without effect on NGF receptor immunoreactivity in the granule neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Present results demonstrate the presence of NGFr IR in sensory nerve corpuscles of the human digital skin , suggesting that NGFr could be involved in the concentration of NGF and in the conveying of this molecule from the cutaneous sources to the cell body of NGF dependent primary sensory neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
A series of recombinant herpes simplex virus ( HSV 1 ) vectors have been constructed that encode either the full length cDNA of the human p 75 NGF receptor ( p75hNGFR ) or truncated forms of the receptor . ^^^ Hence , these HSV 1 vectors give rise to appropriate NGF receptor binding after viral infection . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The neuron like differentiation of PC 12 cells is induced by nerve growth factor ( NGF ) through stimulation of a membrane bound protooncoprotein signaling pathway containing the NGF receptor Trk , the tyrosine kinase Src , and the GTP binding protein Ras . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
For the purpose of developing a cell free binding assay suitable for the large scale screening of receptor agonists and antagonists , the extracellular ligand binding domains of the low affinity NGF receptor ( p75NGFR ) , a splicing variant of the fibroblast growth factor receptor ( FGFR ) , and the PDGF beta receptor ( PDGFR ) were modified by the biotinylation of amino groups . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In cultured rat pheochromocytoma PC 12 cells , ERK 1 and ERK 2 are activated by nerve growth factor ( NGF ) , which also induces rapid association between ERK 1 and the high affinity gp140prototrk tyrosine kinase NGF receptor . ^^^ In the present work , we investigated the possible association between ERKs and the low affinity NGF receptor , p 75 . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The message for the low affinity NGF receptor , p75NGFR , is induced in Schwann cells distal to the injury and is repressed as regenerating axons make contact with these cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In addition to gp145trkB , the pyramidal neuronal cultures expressed transcripts specific for the NT 3 receptor gp145trkC , but not for the high affinity NGF receptor gp140trk or for p75LNGFR , a low affinity receptor for all known members of the NGF family of neurotrophins including the gp145trkB ligands , BDNF and NT 4 . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In rat or cat , the topography of 125I NT 3 binding differed from that reported for 125I NGF binding or for the low affinity NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In this study , our aim was to determine NGF receptor ( NGFr ) status in medulloblastoma specimens and cell lines and to investigate whether NGF could act as a potential differentiating agent for this common pediatric brain tumor . ^^^ In this study , we also examined the effects of retinoic acid , 12 O tetradecanoyl phorbol 13 acetate , and NGF on medulloblastoma cell lines to evaluate their effect on morphological differentiation and NGFr expression . ^^^ Therefore , because of the lack of de novo or induced NGFr expression , it is unlikely that NGF will be useful as a potential therapeutic differentiating agent for medulloblastomas . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The absence of NGFr immunoreactive neurons within the caudate nucleus , and the paucity of such neurons in the putamen , suggests that NGF receptors play a limited role in primate neostriatal development . ^^^ NGF receptor ( p 75 ) immunoreactivity in the developing primate basal ganglia . ^^^ These data indicate that NGF receptor mediated mechanisms may underlie developmental processes within the primate basal ganglia . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The patterns of neurotrophin mRNA localization were different from those of mRNAs for the low molecular weight NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In order to examine the relationship between the action of opioid neurotransmitters and growth factors in the regulation of brain development , we have studied the long term effect of the opiate antagonist naltrexone ( NTX ) on the content of nerve growth factor ( NGF ) in cortex , hippocampus , septum and neostriatum , and on NGF receptor ( NGFRs ) levels in cortical membranes . 50 mg / kg NTX treatment induced a decrease in the number of 125I NGF high affinity binding sites , without detectable changes in NGF levels . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Pretreatment with an antibody against the p 75 low affinity NGF receptor blocked the NGF induced excitations . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To dissect the signal transduction pathways which lead to NGF actions , we have assessed the relative roles of NGF receptor , Src , Ras , and Raf activities in mediating specific gene inductions . ^^^ From these and previous results , we suggest a model in which a linear order of NGF receptor , Src , Ras , and Raf activities is used by NGF to elicit gene inductions . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The mechanism of low affinity NGF receptor ( LNGFR ) truncation was investigated in cultured Schwann cells . ^^^ Affinity labeling of Schwann cells with 125I NGF or metabolic labeling with 35S cysteine showed that truncated NGF receptor ( NGF Rt ) was derived from the cell surface form of the receptor . ^^^ Addition of full length , exogenous NGF receptor ( M ( r ) = 80 kDa ) to Schwann cell membranes resulted in cleavage of the exogenous substrate to NGF Rt . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
At elevated concentrations , beyond the saturation of its high affinity receptors , NT 3 supports the survival of some E 11 sympathetic neurons , an effect that might be mediated by the high affinity NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Developmental expression of trkB and low affinity NGF receptor in the rat retina . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In addition , ngfR ( low affinity NGF receptor ) gene expression was present in 9 out of 25 cases . ^^^ The simultaneous presence of mRNA related to both forms of the NGF receptor , while not proving the presence of a functional receptor , indicates the existence of a sub set of neuroblastoma cells potentially responsive to NGF . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Radiolabeled NGF was found to be localized to specific areas corresponding to p 75 NGF receptor distribution in the hippocampus and cortex by autoradiography . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The retinoid produced a two to threefold increase in level of p75LNGFR mRNA ( the low affinity NGF receptor ) , without altering expression of the trk protoon cogene ( the high affinity NGF receptor carrying tyrosine kinase activity ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
This was associated with a transdifferentiation of Muller cells into an injury associated glial fibrillary acidic protein ( GFAP ) expressing phenotype , and an up regulation of the low affinity NGF receptor p75NGFR on both RGC and Muller cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Expression of mRNA for the trk protooncogene , encoding a tyrosine kinase receptor essential for functional NGF signal transduction , and mRNA for the low affinity NGF receptor ( LNGFR ) was examined in 45 neuroblastomas and 3 benign ganglioneuromas using Northern blot analysis . ^^^ It is concluded that neuroblastomas coexpressing mRNA for both NGF receptor subtypes are favorable tumors likely to differentiate or regress spontaneously or respond to conventional therapy . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Both high affinity and low affinity NGF receptors ( NGFR ) are expressed on human B lymphocytes . ^^^ In view of the structural similarities of the p 75 NGFR and CD 40 we examined whether NGF may also be involved in the regulation of IgE production . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
This response was apparent by 24 h , did not occur with NGF treatment or when vector or TrkA NGFR were expressed and was reversed by dexamethasone pretreatment . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Our cellular data support the concept that staurosporine acts to inhibit the NGF receptor Trk ( Nye et al . : Mol Biol Cell 3 : 677 686 , 1992 ) , but that downstream steps can be activated by the higher concentration of staurosporine to bypass Trk and lead to neurite generation . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Support for the latter hypothesis comes from our recent observations of co expression of estrogen receptor mRNA with the mRNAs for the neurotrophins and their receptors ; differential and reciprocal up regulation of estrogen and NGF receptor mRNA and protein expression by estrogen in adult female rat sensory neurons , PC 12 cells ; and cerebral cortical cultures ; and putative estrogen response elements in the NGF , BDNF , trkA and p 75 genes . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The high affinity NGF receptor is thought to be a complex of two receptors , gp 75 and the tyrosine kinase TrkA , but direct biochemical evidence for such an association had been lacking . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Nerve growth factor ( NGF ) , brain derived neurotrophic factor ( BDNF ) , and neurotrophin 3 ( NT 3 ) selectively bind to distinct members of the Trk family of tyrosine kinase receptors , but all three bind with similar affinities to the neurotrophin receptor p 75 ( p75NTR ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Brain derived neurotrophic factor is a ligand for the low affinity NGF receptor , p 75 , and for the high affinity neurotrophin receptor , trkB . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We now report that trkA , the NGF receptor tyrosine kinase , is involved in the acute activational process that leads to the first ovulation . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We have investigated the role of trkA , the tyrosine kinase NGF receptor in mediating the survival response of embryonic chicken neurons to NGF . ^^^ The failure of injected ciliary neurons to show an NGF promoted survival response is not due to absence of the low affinity NGF receptor , p 75 , in these neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To investigate whether the failure of axonal regeneration could be related to a lack of NGF receptor production by Schwann cells , we examined the expression of p 75 NGF receptors by Bngner bands immunocytochemically . ^^^ In comparison with other types of peripheral neuropathy , p 75 NGF receptor expression appeared to take place normally . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
This discovery suggests that the signaling NGF receptor , TrkA , can be used as a marker for nociceptive neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
HTLA 230 , an NB cell line isolated from a patient with advanced disease , and its NGF receptor ( trkA ) expressing derivative ( 18 10 ) cells , consistently disseminated to the liver , the adrenal gland , and the bone marrow , but not the lungs . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The high affinity NGF receptor , trkA , is selectively expressed by nociceptive sensory neurons particularly those containing sensory neuropeptides such as substance P and CGRP . 4 . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Furthermore , colocalization studies show that many BDNF expressing sensory neurons also express trk A , the high affinity NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Hsp 150 delta NGFRe , harvested from the culture medium , inhibited the cross linking of [ 125I ] NGF to authentic NGFR on the surface of human melanoma cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The R 3 peptide had no effects on cell survival , cell binding or uptake of [ 125 ] NGF , affinity cross linking of [ 125 ] NGF to p 75 ( NGFR ) or trkA monomers and homodimers , of NGF mediated trkA monomer tyrosine phosphorylation . ^^^ The studies implicate a role for a highly conserved motif of p 75 ( NGFR ) in the downstream modulation of NGF mediated neurite growth . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Cek 8 and low affinity NGF receptor ( LNGFR ) are expressed at high levels on the chick spinal motoneurons of the brachial and lumbar segments from embryonic day ( E ) 5 to E 7 , but weakly on the motoneurons of the non limb innervating segments . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We performed high sensitivity flow cytometry and Western blotting to study the expression of the low affinity NGF receptor ( p75NGFR ) and of the transmembrane tyrosine kinase ( Trk ) family of high affinity receptors for the different neurotrophic factors on Epstein Barr virus ( EBV ) transformed human B lymphocytes . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The first part of this paper presents and discusses experiments which were performed in our laboratory to study the NGF receptor response after intracerebral NGF treatment in vivo . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Binding of 125I NGF to ROS cells displayed the properties of a low affinity NGF receptor ( dissociation constant Kd approximately 10 ( 9 ) M ) . ^^^ In agreement with this result , the mRNA encoding the low affinity NGF receptor ( LNGFR ) was detected in ROS 17 / 2 . 8 cells , unlike trkA transcripts which encode the high affinity receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Nerve growth factor ( NGF ) mediated protection of neural crest cells from antimitotic agent induced apoptosis : the role of the low affinity NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Mutants of NGF that do not bind to the neurotrophin receptor p75NTR or other nonneuronal growth factors were not able to enhance the invasion of 70W melanoma cells . ^^^ When 70W cells were exposed to antisense oligonucleotides directed against p75NTR mRNA , there was a reduction in NGF and NT 3 binding , and the neurotrophins failed to enhance Matrigel invasion . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To test the molecular nature of the NGF receptor responsible for the ability of NGF to rescue septal cholinergic neurons following axotomy , we infused polyclonal antibodies that act as specific agonists of trkA ( RTA ) into the lateral ventricle of fimbria fornix lesioned animals . ^^^ Rats receiving chronic intraventricular infusions of RTA showed significantly more low affinity NGF receptor immunoreactive ( p75NGFR IR ) neurons on the lesioned side than did control animals 2 weeks following unilateral fimbria fornix lesion . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The contribution of nerve growth factor ( NGF ) to inflammatory hyperalgesia potentially could be mediated by any of the three peripheral cell types that express trkA , the high affinity NGF receptor : inflammatory cells , sympathetic neurons , and primary sensory neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We further focused on the mechanisms by which dexamethasone affects the expression of the low affinity NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Reexpression of NGFR in motor neurons after trauma reflects an increased demand of neuotrophic factors to support the post trauma survival of NGF dependent neurons . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The situation is complex , however , in that NT 3 ( / ) mutant mice show far greater neuron loss than mice deficient in the NT 3 receptor TrkC , suggesting that NT 3 may support many DRG neurons via actions on the NGF receptor TrkA . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Since NGF exerts its effects via p75NTR and TrkA receptors coexpressed in vast majority of these neurones and down regulated without NGF treatment after injury , in this study we aimed to examine how does the lesion to the cholinergic tracts affect distribution of both types of receptor proteins in damaged fibres . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Parallel studies were performed in rat dorsal root ganglion cultures ; suramin potentiated neurite outgrowth and activated the NGF receptor on these cells . ^^^ This finding of specific patterns of tyrosine phosphorylation of cellular proteins in response to suramin treatment demonstrated that suramin is a partial agonist for the NGF receptor in both PC 12 cells and dorsal root ganglion neurons . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
This study examines how the family of neurotrophin receptor tyrosine kinases ( Trks ) participates in the regeneration and replacement of olfactory neurons within the adult rat olfactory neuroepithelium . mRNA and protein products representing the high affinity nerve growth factor ( NGF ) receptor Trk A , its family members Trk B and Trk C , and the low affinity NGF receptor ( INGFR ) are all detected within both mature and regenerating olfactory neuroepithelium and within primary cultures of olfactory neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Nerve growth factor ( NGF ) added to SH SY5Y cells stably transfected with the NGF receptor TRK A ( SH SY5Y / trk ) induces a similar differentiated phenotype . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To verify whether the reduction of enzyme activity can be ascribed to changes in or ablation of projecting neurons , we carried out immunohistochemical analysis of ChAT and low affinity NGF receptor ( p75NGFR ) in the basal nucleus of control and MAM treated animals . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The NGFR expressed on Schwann cells could mediate NGF to support and induce the axon regeneration in the central nervous system . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The NGF gene is linked genetically to high blood pressure in the spontaneously hypertensive rat ( SHR ) and there exists a mutation in the SHR low affinity NGF receptor ( LNGFR ) gene . 2 . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
TrkC , the high affinity NT 3 receptor , and trkA , the high affinity NGF receptor , are both expressed from the early OLP through the mature OL stage . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Nerve growth factor ( NGF ) provides critical trophic support to the cholinergic basal forebrain neurons that express high levels of the low affinity NGF receptor ( p75NGFR ) in the adult rat brain . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We have found evidence that part of the survival promoting activity of GM 1 is dependent on the presence , dimerization , and activation of the Trk NGF receptor tyrosine kinase and that GM 1 causes a detectable increase in Trk receptor autophosphorylation . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
These observations indicate that target derived NGF is required for the maintenance of NGF receptor expression in adult rat DRG neurons and that non target derived factors can appropriate this function following peripheral nerve injury . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
These cells maintain the phenotype of Schwann cells as determined by morphology and staining for GFAP , S 100 , p 75 NGF receptor , laminin , and MAG production in co culture with DRG neurons . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The zinc finger transcription factor Zif268 / Egr 1 is essential for Schwann cell expression of the p 75 NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
These findings may give some clues about the function of the bone marrow stromal cells , which are known to be involved in the homing and recirculation of hemopoietic cells ; in addition , the presence and distribution of NGFR in the bone marrow stroma may support the recent evidence of a co stimulatory effect of NGF in early hematopoiesis . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Confocal microscope images of double staining for neuritic markers ( neurofilament or low affinity NGF receptor ) and the astrocytic marker glial fibrillary acidic protein ( GFAP ) demonstrated that regenerating neurites crossed dense buildups of astrocytic processes at the edges of NGF producing grafts and were in apposition with astrocytic processes within NGF producing grafts . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In this study we measured the concentration of nerve growth factor ( NGF ) and the expression of NGF and the low affinity NGF receptor ( NGF r ) mRNA in the central nervous system ( CNS ) of rats affected by experimental allergic encephalomyelitis ( EAE ) during the acute phase of the disease . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
An understanding of the structure function relationship of nerve growth factor ( NGF ) requires precise knowledge of all the residues and regions that participate in NGF receptor binding , receptor activation , and biological activity . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In contrast , the level of trkA , the NGF receptor , regulates these properties in caudate putamen cholinergic neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The migration of Schwann cells along embryonic nerves is reportedly regulated by the 75 kd neurotrophin receptor ( p75NTR ) and one of its cognate ligands , nerve growth factor ( NGF ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Gene transcripts for the high affinity NGF receptor trkA were found exclusively in non germ cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Postnatal changes in the expression of the trkA high affinity NGF receptor in primary sensory neurons . ^^^ In development approximately 70 80 % of dorsal root ganglion ( DRG ) cells are dependent on nerve growth factor ( NGF ) for their survival , while in the adult only some 40 % of DRG cells express the high affinity NGF receptor , trkA . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Glycosylation of rat NGF receptor ectodomain in the yeast Saccharomyces cerevisiae . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In this immunohistochemical study we investigated the expression of low affinity NGF receptor ( p75NGFR ) in peripheral nerves from 16 patients with type 1 or type 2 diabetes mellitus . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NGF binding studies indicated that ethanol exposure ( 400 mg / dl for 4 days ) reduced the density of the low affinity ( p 75 ) NGF receptor on PC 12 cells , but had no effect on the density of the high affinity NGF receptor . ^^^ Low affinity NGF binding is mediated by the p 75 component of the NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Low affinity NGF receptor expression in CMT1A nerve biopsies of different disease stages . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
It is , however , unknown whether other homologous neurotrophins also act outside the nervous system , and whether activation of basophils by NGF requires interaction with trk tyrosine kinase receptors , the low affinity NGF receptor ( LNGFR ) , or both . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Human recombinant NGF mediated phosphorylation of a 140 kDa Trk NGF receptor in the TSU pr 1 cell line was inhibited by treatment with 100 nM K252a . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Interestingly , those cells could hardly make terminal differentiation in the presence of NGF and continued to grow without changing their round shape , although NGF receptor as well as MAP kinase appeared to be activated . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Furthermore , D283MED cells engineered to express the human p 75 NGF receptor ( D283p75 ) also did not undergo apoptosis . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Recently , a monoclonal antibody to the low affinity nerve growth factor ( NGF ) receptor , 192IgG , coupled to a cytotoxin , saporin , has been described as an efficient and selective immunotoxin for the NGF receptor bearing cholinergic neurons in rat basal forebrain . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We have studied the postnatal expression of neurotrophins , their cognate high affinity trk receptors and the low affinity NGF receptor ( p75LNGFR ) in the rat adrenal gland using RT PCR . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
These results suggest that the NGF effect is mediated by the high affinity NGF receptor , Trk A and that neurotrophin binding to the low affinity neurotrophin receptor , p 75 ( NTR ) , alone does not affect the promoter activity . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Inhibition of neuronal differentiation is accompanied by significant changes in the protein and mRNA expression pattern of the high affinity NGF receptor ( trkA ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
K 252a inhibits the effect of NGF on p 140 ( trk ) cells but not on p 75 ( NGFR ) cells ; brain derived neurotrophic factor stimulates calcium uptake in p 75 ( NGFR ) cells but not in p 140 ( trk ) cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To analyze how motoneurons acquire regional specification along the anterior posterior ( A P ) axis and in the transverse plane , we observed the expression of Cek 8 , Islet 1 , SC 1 and low affinity NGF receptor on ectopic motoneurons induced by implanting a supernumerary notochord or floor plate . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To examine their roles in the TRK NGF receptor , these residues ( Tyr 670 , Tyr 674 , and Tyr 675 ) were mutated singly and in all combinations to phenylalanine and stably expressed in Trk deficient PC12nnr5 cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
For nerve growth factor ( NGF ) , these are p75NTR ( p 75 ) and p140trk ( referred to as trk or trkA ) , which binds both BDNF and neurotrophin ( NT ) 4 / 5 , and trkC receptor , which binds only NT 3 . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Using an oxidation sensitive compound , dihydrorhodamine , we measured ROS formation in a central nervous system derived neuronal cell line ( GT 1 1 trk ) and in superior cervical ganglion neurons , both of which express the transmembrane NGF receptor tyrosine kinase , trkA . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In summary , although some cellular events promoted NGF accumulation in cell bodies , distal axons represented by far the principal site of NGF receptor interaction at steady state as a result of a low retrograde transport rate . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The mechanism ( s ) underlying nerve growth factor ( NGF ) mediated rescue of neurons from apoptosis is poorly understood , although it is well established that the high affinity NGF receptor ( TrkA ) plays a pivotal role in mediating NGF effects . ^^^ The report that the low affinity NGF receptor ( p75NGFR ) can induce apoptosis prompted us to analyze the role played by a putative p75NGFR associated signal transduction element , the transcription factor nuclear factor kappa B ( NFkappaB ) , in the modulation of apoptosis in PC 12 cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Following immunocytochemical staining , we counted the low affinity NGF receptor p 75 positive DRG neurons in MRL mice . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
A role for the local activation of the high affinity NGF receptor trkA was suggested by a strong inhibition of the turning response by ( 1 ) the addition of an antibody against the extracellular portion of trkA , ( 2 ) the elevation of the background concentration of NGF to saturate trkA , or ( 3 ) the presence of a concentration of the drug K252a that inhibits trkA activation . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Intracellular application of a phosphopeptide , Trk 490 , which prevents the binding of the adaptor protein shc to the activated NGF receptor , so blocking p 21 ras activation , reduced 1 ( max ) . ^^^ Together , these results suggest that NGF receptor tyrosine kinase activation of p 21 ras is likely to be involved in the tonic regulation of VDCCs in DRG neurons . ^^^ This effect appears largely to involve NGF receptor tyrosine kinase activation of p 21 ras . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Antibodies against low affinity NGF receptors ( p75NTR ) mimicked the effects of NGF , suggesting that this receptor , which is transiently expressed at high levels in postmitotic granule neurons , is involved in apoptosis signaling . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Limiting consideration to the role of p75NTR in cell death , a theory is proposed that is based on the following postulates : ( 1 ) that p75NTR displays a pro apoptotic intrinsic ( ligand independent , Trk independent ) receptor effect ( IRE ) , which is inhibited by ligand binding ; ( 2 ) that p75NTR and TrkA exhibit mutual repression of signaling ; and ( 3 ) that p75NTR and TrkA are required for the efficient generation of high affinity NGF binding sites . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
This down regulation does not occur in PC12nnr5 cells , which lack the p 140 ( trk ) NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Both cortical and basal forebrain explant cultures express the NGF receptor , TrkA . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Our results support the hypothesis that p75NTR , when it is not bound by NGF , may play a role in neuronal selection during embryonic development and suggest that neuroblastomas may arise from immature neuroblasts that escape programmed cell death . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Thus , although NGF signals retrogradely to mediate nuclear responses , the magnitude of these responses differs as a function of the spatial location of the activated NGF receptor : ligand complex . ^^^ To examine the cellular mechanisms whereby distally derived growth factors regulate nuclear responses in neurons , we have utilized compartmented cultures of sympathetic neurons to examine the regulation of two nerve growth factor ( NGF ) inducible genes , tyrosine hydroxylase ( TH ) and p 75 neurotrophin receptor ( p75NTR ) . ^^^ These studies demonstrate that NGF can signal retrogradely to mediate the induction of TH and p75NTR mRNAs . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In this NGF responsive subpopulation of DRG neurons , estrogen regulates expression of the NGF receptor genes and may increase the sensitivity of these cells to the neurotrophin . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Nerve growth factor ( NGF ) treatment of rat PC 12 pheochromocytoma cells results in an increase in the tyrosine phosphorylation of the NGF receptor , TrkA , leading to differentiation to a neuronal phenotype . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We also examined the distribution of the low affinity NGF receptor , p75NGFR , on NGF responsive neurons that are localized in the septum and the nucleus of Meynert , which receive the respective trophic support from the hippocampus and the cortex . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Recovery of ChAT immunoreactivity in axotomized rat cholinergic septal neurons despite reduced NGF receptor expression . ^^^ Here we provide evidence that the recovery of ChAT immunoreactivity after axotomy is not accompanied by a similar recovery of NGF receptor expression in these neurons . ^^^ In addition , there was a persisting loss of p75NTR immunoreactive and NGF immunoreactive medial septal neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Inhibitors of other second messengers , including staurosporine , pertussis and cholera toxins , failed to have an effect on the transport of the NGF receptor complex in both cell types . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Molecular modeling studies predict that Zn2+ binding to NGF will induce structural changes within domains of this neurotrophin that participate in the recognition of TrkA and p75NTR . ^^^ We demonstrate here that Zn2+ alters the conformation of NGF , rendering it unable to bind to p75NTR or TrkA receptors or to activate signal transduction pathways and biological outcomes normally induced by this protein . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To identify nerve growth factor ( NGF ) target cells in normal and pathologic human lymphoid tissues , we have studied the expression of the low affinity NGF receptor ( p75LNGFR ) and the high affinity tropomyosin related kinase NGF receptor ( TrkA ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Pinealocyte synaptic ribbons ( SR ) and dense cored vesicles ( DCV ) were quantitated in mice that have developmental deficiencies in the sympathetic innervation of the pineal gland due to a null mutation for the p 75 low affinity NGF receptor ( NGFR ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
This study examined whether ethanol exposure of the neonatal rat cerebellum altered the levels of either NGF or the expression of p 75 and trkA , which are two components of the NGF receptor . ^^^ Immunohistochemical labeling techniques indicated that both the p 75 and trkA NGF receptors were expressed on Purkinje cell dendrites in the developing cerebellum , with posterior lobules expressing higher levels of p 75 and trkA NGF receptor , compared with anterior lobules . ^^^ These results suggest that ethanol could interfere with neurotrophic support of Purkinje cells by reducing the levels of available NGF receptor . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We have investigated the expression of the signal transducing NGF receptor trkA on mRNA and protein level during development of chick sympathetic neurons obtained from lumbosacral , paravertebral chain ganglia between embryonic days ( E ) 6 . 5 and 10 . ^^^ These data provide evidence that sympathetic neurons upregulate the NGF receptor trkA and in this way acquire NGF dependency . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To confirm this , the relationship of the transported NGF to the level of sensory neurone expression of the NGF receptor proteins was analysed . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Furthermore , bFGF induced expression of the high affinity NGF receptor ( trkA ) gene . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate NGF receptor expression and NGF production and release by human CD4+ cells clones , which have primary relevance in modulating inflammatory events through their different subsets of functional phenotypes . ^^^ RESULTS : Our data have shown that in unstimulated conditions , human CD4+ T cell clones express both immunoreactivity for NGF and the TrkA NGF receptor irrespective of their cytokine profile . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The poor response of mi / mi CMCs to NGF was attributed to the deficient expression of p 75 NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To determine if human mast cells express the NGF receptors , TrkA tyrosine receptor and the low affinity NGF receptor ( LNGFR ) , we first analyzed the mRNA expression by RT PCR of TrkA and LNGFR in a human mast cell line ( HMC 1 ) and in human mast cells cultured in the presence of stem cell factor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Mutants of NGF that do not bind to the neurotrophin receptor p75NTR or other nonneuronal growth factors were not able to enhance the invasion of 70W melanoma cells . ^^^ When 70W cells were exposed to antisense oligonucleotides directed against p75NTR mRNA , there was a reduction in NGF and NT 3 binding , and the neurotrophins failed to enhance Matrigel invasion . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Steady state expression of transcripts for neurofilament ( NF ) proteins ( NF L , NF H ) and the high affinity NGF receptor , trkA was decreased by diabetes in the contralateral ganglia to the crush ( all P < 0 . 05 ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Normal human keratinocytes synthesize and release nerve growth factor ( NGF ) and express both the low and the high affinity NGF receptor . ^^^ We report here that apoptosis is induced in normal human keratinocytes in culture by blocking endogenous NGF signaling with either anti NGF neutralizing antibody or K 252 , a specific inhibitor of the tyrosine kinase high affinity NGF receptor . ^^^ These findings show that autocrine NGF acts as a survival factor for human keratinocytes in vitro through its high affinity NGF receptor , possibly by maintaining constant levels of Bcl 2 . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The present data give evidence for the expression of TrkA , the so called high affinity NGF receptor , and of p75NTR , the so called low affinity NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To clarify the role of the common neurotrophin receptor p 75 in modulating the survival response of sensory and sympathetic neurons to NGF at different stages of development , we compared the actions of wild type NGF with a mutated NGF protein that binds normally to TrkA , the NGF receptor tyrosine kinase , but has greatly reduced binding to p 75 . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The fact that increased nerve growth factor ( NGF ) protein levels were found throughout brains of Alzheimer ' s disease patients led us to investigate neurotrophin synthesis in a human microglial cell line showing typical properties of human microglial cells , including expression of neurotrophins such as NGF , as well as the NGF receptor trkA and the low affinity neurotrophin receptor p 75 . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To address the potential role of p75NTR in axon growth , we have generated a new line of hybrid mice overexpressing NGF but lacking functional p75NTR expression . ^^^ These results show that p75NTR is not vital for new growth of NGF sensitive sympathetic and sensory axons and that immunohistochemical detection of NGF at sympathetic axons requires the functional expression of p75NTR . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Mobility of TrkA is regulated by phosphorylation and interactions with the low affinity NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Mutants of NGF that do not bind to the neurotrophin receptor p75NTR or other nonneuronal growth factors were not able to enhance the invasion of 70W melanoma cells . ^^^ When 70W cells were exposed to anti sense oligonucleotides directed against p75NTR mRNA , there was a reduction in NGF and NT 3 binding , and the neurotrophins failed to enhance Matrigel invasion . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The presence of Nerve Growth Factor ( NGF ) and the expression of low and high affinity NGF receptor were investigated in prenatal , postnatal and pregnant rats . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The neurotrophic activity of APP was independent of the TrkA NGF receptor because neither phospholipase C gamma 1 nor TrkA exhibited tyrosine phosphorylations with APP treatment . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
These results reveal a central specific role of the high affinity NGF receptor during neurodegeneration in AD . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Neurotrophin expression was not unique to adult tissues since pancreatic cells transiently expressed p 75 , the NGF receptor Trk A , and NGF during development . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
A role of the thymus and thymosin alpha 1 in brain NGF levels and NGF receptor expression . ^^^ Using neonatal rats we investigated the role of the thymus and thymosin alpha 1 ( T alpha 1 ) in brain NGF levels , NGF receptor ( p75NGFr ) expression , as well as the activity of choline acetyl transferase , a cholinergic enzyme regulated by NGF . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Next , we investigated the tyrosine phosphorylation of NGF receptor TrkA and the EGF receptor ( EGFR ) as well as mitogen activated protein kinase ( MAPK ) , which is a key protein in the intracellular signal transduction pathway . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Here we report that activation of the TrkA NGF receptor in oligodendrocytes negates cell death by the p 75 receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Administration of NGF induced a short lasting hypertrophy of low affinity NGF receptor immunoreactive neurons within the nucleus basalis magnocellularis ( NBM ) , the horizontal limb of the diagonal band of Broca , and the medial septum . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Low affinity NGF receptor expression in the central nervous system during experimental allergic encephalomyelitis . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Developmental expression of the NGF receptor p140trk in the septohippocampal system of the rat : a quantitative analysis . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
As determined by transient expression , two NGF response elements ( REs ) were required for transcription induced by NGF in SH SY5Y cells with stable expression of an exogenous NGF receptor TRK A gene ( SH SY5Y / trk ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
These axons are responsive to the neurotrophin nerve growth factor ( NGF ) , in that they possess the transmembrane receptors p140proto trkA and p75neurotrophin receptor ( NTR ) which bind NGF . p75NTR deficient mice display reduced patterns of sympathetic innervation of the pineal gland and sensory innervation of the skin ( Lee et al . , 1992 , 1994a ) . ^^^ Four lines of mice were used : wild type C57Bl / 6 mice , transgenic mice overexpressing NGF in the brain , p75NTR deficient mice , and hybrid mice which overexpress NGF in the brain but lack p75NTR expression . ^^^ These data reveal that while p75NTR expression may be a critical factor for initiating axon growth along the extracerebral vasculature during postnatal development , the sympathetic and sensory nervous systems display a remarkable degree of NGF induced axonal plasticity , such that increased levels of NGF can ameliorate perturbed patterns of arterial innervation in p 75 deficient mice . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Here we report the effects of partial immunolesions on the levels of choline acetyltransferase ( ChAT ) activity and NGF receptor mRNA levels in the basal forebrain of 3 and 30 month old rats . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Approximately 6 % of the cells were stained with antibodies for the low affinity NGF receptor , a neural crest cell marker . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To test this , double label immunohistochemistry for the NGF receptor ( trkA ) and sympathetic baskets was performed . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In addition , our results show that MKP 1 mRNA is induced after NGF stimulation of fibroblasts expressing the NGF receptor TrkA , suggesting that MKP 1 is upregulated after activation of the TrkA receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In transactivation assays , C / EBPbeta activity was stimulated by NGF receptor signaling . ^^^ Our data identify the C / EBPbeta protein and gene as direct downstream targets of the NGF receptor and suggest a role for C / EBPbeta in neurotrophin signaling in the brain . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Role of the low affinity NGF receptor ( p 75 ) in survival of retinal bipolar cells . ^^^ Both nerve growth factor ( NGF ) and an antibody ( anti REX ) which interferes with binding to the 75 kD low affinity neurotrophin receptor ( p75NTR ) eliminated BDNF promoted survival , but had no effect on FGF 2 mediated survival . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The genes coding for nerve growth factor ( NGF ) , brain derived neurotrophic factor ( BDNF ) and their common low affinity receptor p 75 ( neurotrophin receptor ) ( p75NTR ) were shown to be expressed in C2C12 myoblasts and downregulated during myogenic differentiation and fusion into myotubes . ^^^ Enhanced muscle differentiation was also seen in p75NTR overexpressing C2C12 myoblasts which maintained high levels of receptors but ceased to produce NGF during differentiation . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In our preceding report , we have shown that nerve growth factor ( NGF ) and its low affinity receptor ( p75NTR ) are expressed in C2C12 myoblasts and downregulated during myogenic differentiation . ^^^ Furthermore , NGF affects myogenic differentiation and cell growth via p75NTR and downregulation of p75NTR is essential for myogenic differentiation ( Seidl et al . , 1998 ) . ^^^ Here we show that NGF and p75NTR are regulated by mechanisms preceding terminal differentiation in myogenic cells . ^^^ Downregulation of NGF and p75NTR occurred as a consequence of increasing cell density , an important trigger for the onset of myogenic differentiation . ^^^ FGF 2 and FGFR 1 were shown to be present in C2C12 cells and exogenous FGF 2 induced NGF and p75NTR expression , implying that FGF / FGFR signaling is an upstream regulator of the NGF / p75NTR system . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
CONCLUSIONS : The NGF receptor is present on the human ocular surface . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Induction of apoptosis through NGF / p75NTR in human glioma cells treated with propentofylline ] . ^^^ Recent studies demonstrated the presence of apoptosis induced via NGF / p75NTR in developmental neuron , oligodendroglia . ^^^ In the present study , to examine whether signal cascade via NGF / p75NTR participates the apoptosis in human glioma cells treated with PPF , we investigated the immunohistochemical study using anti mouse monoclonal antibody , immunoprecipitation and northern blot analysis for p75NTR , NF kappa B in human glioma cells ( U251MG , T98G , U 87 ) treated with PPF . ^^^ According to above results , it is assumed that apoptosis in human glioma cells treated with PPF was induced via NGF / p75NTR . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Exposure of human neuroblastoma cells ( IMR 32 ) to a peptide mimic of the cytoplasmic amphiphilic domain of the common neurotrophin receptor ( p75NTR 367 379 ) resulted in enhanced nerve growth factor ( NGF ) mediated inhibition of cell invasion in vitro . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Nerve growth factor ( NGF ) , neurotrophin 3 , neurotrophin 4 , and brain derived neurotrophin exert their survival effect by binding to two transmembrane receptor types : trk receptors , which exhibit binding specificity , and the p75NTR receptor , which binds all neurotrophins . ^^^ The present study focused on the p 75 receptor and shows that the percentage of neurons expressing p75NTR also increase in NGF ganglia , but only by 10 % . ^^^ These results show that in sensory systems , target derived NGF modulates the level of p75NTR receptor expression , and in so doing , may act to regulate the formation of functional receptor complexes and subsequent trophic action . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
By RT PCR , we detected TrkAI transcripts encoding for the high affinity NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Western blot analysis of these cells revealed tyrosine phosphorylation of the high affinity NGF receptor ( TrkA / gp140 ) and activation of a downstream signal cascade element , ERK 1 / MAP kinase . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The in vivo targeting efficacy of small molecule analogs of nerve growth factor ( NGF ) that bind the NGF receptor p 140 TrkA was evaluated and compared with that of a high affinity anti TrkA monoclonal antibody ( Mab 5C3 ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Finally , NGF recently was shown to activate the neurotrophin receptor p 75 ( p75NTR ) , a receptor with no intrinsic catalytic activity and with similarities to members of the tumor necrosis factor receptor family . ^^^ During normal development , the activation of p75NTR by NGF actually kills cells in the central nervous system . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
There was a close relationship between sCD 27 and soluble TNF alpha , another molecule belonging to the NGF receptor superfamily . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The purpose of this investigation was to determine whether NGF equally affects the immunolocalization of L 1 on both sympathetic and sensory axons , and whether the functional expression of the p 75 neurotrophin receptor ( p75NTR ) is required for the immunodetection of this glycoprotein on peripheral axons . ^^^ Two lines of transgenic mice overexpressing NGF among glial cells were used in this study : ( 1 ) one line of mice possessing two normal alleles for p75NTR , and ( 2 ) another line of mice possessing two mutated alleles for p75NTR . ^^^ From these in vivo data , we conclude that collateral branches of sensory axons which invade a NGF rich target area display L 1 immunoreactivity , and that such immunodetection does not require the functional expression of p75NTR . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The pheochromocytoma PC 12 cell line was used as a model system to characterize the role of the p 75 neurotrophin receptor ( p75NTR ) and tyrosine kinase ( Trk ) A nerve growth factor ( NGF ) receptors on amyloid precursor protein ( APP ) expression and processing . ^^^ Displacement of NGF binding to p75NTR by addition of an excess of brain derived neurotrophic factor abolished NGF ' s effects on neurite outgrowth and APP metabolism , whereas addition of brain derived neurotrophic factor alone did not induce neurite outgrowth or affect APP mRNA or protein processing . ^^^ However , treatment of PC 12 cells with C 2 ceramide , an analogue of ceramide , the endogenous product produced by the activity of p75NTR activated sphingomyelinase , mimicked the effects of NGF on cell morphology and stimulation of both APP mRNA levels and APP secretion . ^^^ These findings demonstrate that in PC 12 cells expressing p75NTR and TrkA receptors , binding of NGF to the p75NTR is required to mediate NGF effects on cell morphology and APP metabolism . ^^^ Furthermore , our data are consistent with NGF having specific effects on p75NTR not shared with other neurotrophins . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Low affinity nerve growth factor receptor ( p 75 NGFR ) , if demonstrated in the urothelium , could provide the means for ( 1 ) NGF mediated modulation of the urothelial response to inflammation ; ( 2 ) NGF mediated autocrine / paracrine regulation of urothelial proliferation ; and ( 3 ) p 75 NGFR mediated induction of apoptosis . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In contrast , human bone marrow cells consistently expressed mRNA for trkB ( the BDNF and NT 4 receptor ) and displayed variable expression of mRNA coding for trkA ( the tyrosine kinase NGF receptor ) and LNGFR ( the low affinity NGF receptor ) , whereas mRNA for trkC ( the NT 3 receptor ) was not expressed . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Using the mice which had received unilateral olfactory bulbectomy and in which antibodies to NGF had been continuously infused with into the contralateral olfactory blub , three kinds of analysis were performed : histological analysis of the olfactory epithelium by HE staining , immunohistochemical analysis of the olfactory epithelium using polyclonal antibodies to trk which forms the NGF receptor , and olfactory mediated behavioral analysis with cycloheximide . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Our previous studies have shown that cholera toxin B subunit , which specifically binds to the cell surface ganglioside GM 1 , and GM 1 itself can enhance the action of nerve growth factor ( NGF ) in responsive cells by enhancing the NGF induced autophosphorylation of the high affinity NGF receptor , Trk . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The role of TrkA and p75NTR receptors was investigated in NGF induced potentiation of glutamate release . ^^^ Block of NGF binding to p75NTR using specific blocking antibodies ( REX IgG ) slightly but significantly reduced the effect of NGF . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The timing of mRNA expression after injury and the phenotype of identified trigeminal neurons suggests a complex signaling cascade in which NGF at the injury site regulates NGF receptor expression at the levels of the cell body as well as increases in BDNF expression . ^^^ Radiolabeled NGF was transported in a receptor mediated fashion from the teeth to a subset of neurons in the trigeminal ganglion within 15 h , indicating that these neurons possess NGF receptors ( trk A and / or p75NTR ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Equilibrium binding of 125I nerve growth factor ( 125I NGF ) to cells coexpressing the tyrosine kinase receptor A ( TrkA ) and common neurotrophin receptor ( p75NTR ) , cells coexpressing both receptors where p75NTR is occupied , and cells expressing only p75NTR , revealed reciprocal modulation of receptor affinity states . ^^^ These data are consistent with receptor allosterism , and prompted a search for TrkA / p75NTR complexes in the absence of NGF . ^^^ The heteroreceptor complex of TrkA and p75NTR alters conformation and / or dissociates in the presence of NGF , as indicated by the ability of low concentrations of NGF to prevent heteroreceptor crosslinking . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Two types of receptors mediate the actions of these polypeptides : the Trk family of tyrosine kinase receptors and the so called p 75 low affinity NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Furthermore , the high affinity NGF receptor ( TrkA ) was found at both the transcriptional and protein levels , while expression of the low affinity NGF receptor was detectable at the mRNA level only . ^^^ Immature human mast cells are thus induced to assume a more mature phenotype in vitro in response to NGF , most probably via stimulation of the high affinity NGF receptor expressed on these cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Recent experiments with NGF responsive cells revealed a specific role for the high affinity NGF receptor , TrkA , in the increases in secretory APP processing and a role for the low affinity neurotrophin receptor , p75NTR , in the transcriptional regulation of APP . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The reverse transcription polymerase chain reaction , immunocytochemical staining , and Western blotting revealed that cultured microglia express Trk A , B , and C , and low affinity NGF receptor , LNGFRp 75 . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We also investigated the novel effect of nerve growth factor ( NGF ) treatment on the levels of mRNA and protein of the NGF receptors , trkA and p75NTR , in normal and diabetic rats . ^^^ Treatment with systemic NGF reversed the diabetes induced deficit in p75NTR transcripts and protein within the DRG and left expression levels of trkA unchanged . ^^^ We propose that in sensory neurons of diabetic rats , the ability to capture and retrogradely transport NGF may be impaired because of suboptimal production of p75NTR receptors and that NGF therapy may overcome this deficiency . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
DEX elicited an increase in NGF mRNA and protein levels in the cerebral cortex and hippocampus as well as specific NGF responses , such as TrkA tyrosine phosphorylation in the septum , choline acetyltransferase ( ChAT ) and p 75 neurotrophin receptor ( p75NTR ) immunoreactivity , and a relative number of cholinergic neurons in the medial septum . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Expression of p75NTR decreased the dose dependent NGF mediated proliferation of the TSU pr 1 prostate epithelial cells . ^^^ The greater the degree of expression of p75NTR in the transfected clones , the less the stimulatory effect of exogenous NGF on cell proliferation . ^^^ Furthermore , the ratio of p75NTR to tropomyosin receptor kinase for each clone was inversely correlated with the ability of NGF to stimulate growth of the TSU pr 1 transfectants . ^^^ Conversely , addition of NGF was able to rescue p75NTR expressing clones from undergoing programmed cell death at levels not significantly different from those of mock transfected clones . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NGF binds to tropomyosin receptor kinase A ( TrkA ) and to 75 kDa NGF receptor ( p75NTR ) . ^^^ NGF induces apoptosis in a human neuroblastoma cell line expressing the neurotrophin receptor p75NTR . ^^^ TrkA is responsible for differentiation and survival , but p75NTR , a member of the death receptor family , seems to mediate the apoptotic effect of NGF . ^^^ Here we demonstrate that NGF but not neurotrophin 3 ( NT 3 ) or brain derived neurotrophic factor ( BDNF ) induced apoptosis in p75NTR expressing human neuroblastoma SK N MC cells . ^^^ Because p75NTR and NGF are upregulated in Alzheimer ' s disease , NGF / p75NTR might be involved in neuronal cell death related to the disease . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Recently , a monoclonal antibody to the low affinity nerve growth factor ( NGF ) receptor , 192IgG , coupled to a cytotoxin , saporin , has been described as an efficient and selective immunotoxin for the NGF receptor bearing cholinergic neurons in rat basal forebrain . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To stimulate TrkA and / or p75NTR signaling in PC 12 cells , we used NGF , brain derived neurotrophic factor ( BDNF ) , and NGF in the presence of an excess of BDNF or the monoclonal antibody 192IgG , to block p75NTR binding to NGF . ^^^ When blocking NGF binding to p75NTR with BDNF or 192IgG , on the other hand , NGF effects on APP secretion were abolished . ^^^ These findings suggest that in cells expressing p75NTR and TrkA receptors , binding of NGF to the p75NTR is required to mediate NGF effects on APP secretion . ^^^ Our data are also consistent with a proposed function of the p75NTR in receptor recruitment and `` presentation ' ' of NGF to receptors . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In cultured neonatal sympathetic neurons , p 53 protein levels are elevated in response to both NGF withdrawal and p75NTR activation . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Increased expression of low affinity NGF receptor in rat retinal Mller cells after ischemia and reperfusion . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We have tested the localization and the molecular nature of the NGF receptor ( s ) responsible for this effect by activating cortical trkA receptors in monocularly deprived rats by cortical infusion of a specific agonist of NGF on trkA , the bivalent antirat trkA IgG ( RTA IgG ) . ^^^ These results suggest that NGF action in visual cortical plasticity is mediated by cortical TrkA receptors with p75NTR exerting a facilitatory role . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Within this population , VR 1 mRNA is detected at widely varying levels in both the NGF receptor ( trkA ) positive , peptide producing cells that elicit neurogenic inflammation and the functionally less characterized glial cell line derived neurotrophic factor responsive cells that bind lectin Griffonia simplicifolia isolectin B 4 ( IB 4 ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The present study examined the effect of long term ( 90 days ) daily injections of an estrogen preparation , Premarin ( Wyeth Ayerst , Radnor , PA ) , to ovariectomized adult rats on lumbar DRG high affinity NGF receptor , trkA , mRNA levels , and on beta preprotachykinin ( beta PPT ) mRNA levels , which have been shown to be regulated by NGF . ^^^ These data suggest the possibility that estrogen modulates DRG neuropeptide gene expression and , perhaps , cutaneous sensitivity by regulating NGF receptor gene expression . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The aim of the present study was , using an antibody specific to the high affinity NGF receptor ( trkA ) , to examine immunohistochemically the distribution of the NGF receptor in the mouse olfactory tract , under normal conditions and during regenerative processes . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We have studied the high affinity NGF receptor TrkA , during the regeneration of the tench ( Tinca tinca L . ) optic nerve , using immunocytochemical techniques . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Blocking the interaction of Abeta with p75NTR using NGF or inhibition of NFKB activation by curcumin or NFKB SN 50 attenuated or abolished Abeta induced apoptotic cell death . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
P75NTR expressing BSR cells were permissive for a non adapted fox RV isolate ( street virus ) and nerve growth factor ( NGF ) decreased this infection . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Since neurotrophins are produced in very small amounts in vivo , NGF sensitive cells have to compete for the limited NGF even under physiological conditions , so that normally only less than 10 % of NGF receptors ( NGFR ) are saturated with their endogenous ligand . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Pentosan polysulfate ( PPS ) was used as another polyanionic agent , to compare these two cellular functions ; NGF receptor interaction and disruption in glycolipid metabolism . ^^^ Like suramin and NGF , PPS induced neurite outgrowth from the PC 12 cell line which correlated with tyrosine phosphorylation of the high affinity NGF receptor ( TrkA / gpl40 ) and ERK 1 / MAP kinase . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The extent of p75NTR expression correlated with the capability of NGF to promote cellular invasion and with production of heparanase , an important ECM degradative enzyme . ^^^ Melanoma cells sorted for high p75NTR expression ( p75NTR H cells ) had markedly greater ( 9 to 13 fold increase ) invasive capabilities in response to NGF exposure than those sorted for low p75NTR expression ( p75NTR L cells ) . ^^^ Additionally , NGF induced a 8 to 10 fold increase of heparanase activity in p75NTR H cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In transgenic mice overexpressing NGF centrally and expressing p75NTR , only a few varicose sympathetic axons invade the optic tract after the first month of postnatal life . ^^^ In other transgenic mice overexpressing NGF centrally but lacking p75NTR expression , the incidence of sympathetic axons within this myelinated tract substantially increases . ^^^ These data provide the first direct evidence that sympathetic axons are indeed capable of NGF induced collateral growth into myelinated tracts of mature mammals , and that their continued growth through this microenvironment is markedly enhanced by the absence of p75NTR expression . ^^^ We propose that p75NTR among sympathetic axons may either directly or indirectly limit collateral branching of these fibers in response to increased levels of NGF . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In order to characterize this specific function of p75NTR , we have tested the effects of nerve growth factor ( NGF ) on embryonic motoneurons from control and p75NTR deficient mice . ^^^ NGF antagonizes brain derived neurotrophic factor ( BDNF ) and neurotrophin 3 ( NT 3 ) mediated survival in control but not p75NTR deficient motoneurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The C 6 glioma cell line can synthesize NGF , respond to exogenous application of NGF and stimulate the expression of NGF receptor in an autocrine manner . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
These data suggest that a blockade of NGF bioactivity using a NGF receptor antagonist is capable of blocking neuropathic and inflammatory pain and further support the hypothesis that NGF is involved in signaling pathways associated with these pain states . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To assess axonal growth and synapse formation , acetylcholinesterase histochemistry , an antibody to choline acetyltransferase ( ChAT ) , and three pig positive / rat negative antibodies : bovine 70kD neurofilament ( NF 70 ) , human low affinity NGF receptor ( hNGFr ) , and human synaptobrevin ( hSB ) were used . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
When human trk A cDNA was transfected into a human glioma cell line , U 251MG , which lacks expression of both endogenous trk A and low affinity NGF receptor , the transfectant expressed the exogenous trk A mRNA and a functional high affinity NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In this report , we provide evidence that NGF and BDNF have functionally antagonistic actions on sympathetic neuron growth and target innervation , with NGF acting via TrkA to promote growth and BDNF via p75NTR to inhibit growth . ^^^ Both exogenous and autocrine BDNF mediate this effect via p75NTR because ( 1 ) BDNF does not inhibit growth of neurons lacking p75NTR , ( 2 ) function blocking p75NTR antibodies enhance NGF mediated growth , and ( 3 ) p75NTR / sympathetic neurons grow more robustly in response to NGF than do their wild type counterparts . ^^^ Thus , BDNF , made by sympathetic neurons and / or their target organs , acts via p75NTR to antagonize NGF mediated growth and target innervation , suggesting that sympathetic target innervation is determined by the balance of positively and negatively acting neurotrophins present in developing and potentially mature targets . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To investigate a putative role of nerve growth factor ( NGF ) in central plasticity linked to chronic pain , modifications in immunoreactivity ( IR ) for the high affinity NGF receptor , TrkA , were studied at spinal levels in a rat model of inflammatory chronic pain , adjuvant induced arthritis ( AIA ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
After the recordings , forebrain transplants and aged brains were immunocytochemically stained for the low affinity NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To determine the role of the p 75 neurotrophin receptor ( p75NTR ) in sympathetic neuron development , we crossed transgenic mice with mutations in p75NTR , nerve growth factor ( NGF ) and neurotrophin 3 ( NT 3 ) . ^^^ Thus , without p75NTR , NT 3 substitutes for NGF , suggesting that p 75 alters the neurotrophin specificity of TrkA in vivo . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Whereas the N 4 , L 38 , and NGFbeta peptide domains present within pro NGF were previously shown to induce phosphorylation of the high affinity NGF receptor , tropomyosin receptor kinase ( Trk ) , only the NGFbeta moiety was able to stimulate anchorage independent growth of prostate tumor cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Ciliary neurotrophic factor enhances the expression of NGF and p 75 low affinity NGF receptor in astrocytes . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To determine whether this action of NGF is mediated by the p140TrkA NGF receptor ( a member of the Trk tyrosine kinase family ) we used a murine basal forebrain cholinergic cell line , SN 56 , stably transfected with rat trkA cDNA . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Identical results were obtained when a specific agonist of the NGF receptor TrkA , the bivalent anti rat TrkA IgG ( RTA ) , was infused into the cortex in place of NGF . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Signal transduction of nerve growth factor ( NGF ) and its TrkA and p75NTR receptors ] . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NGF receptor ( TrkA and p75NGFR ) expression was investigated in human thymuses , including normal thymuses , thymic hyperplasias , thymomas and thymic carcinomas . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Nerve growth factor ( NGF ) exerts its pro apoptotic effect via the P75NTR receptor in a cell cycle dependent manner . ^^^ Nerve growth factor ( NGF ) , the prototypic member of the neurotrophin family of growth factors , exerts its action via two receptors , P75NTR and TrkA , the expression of which varies at the cell surface of neuroblastoma cells ( SH SY5Y cells ) in a cycle phase specific manner . ^^^ NGF was pro apoptotic on growing cells expressing preferentially P75NTR and exhibited a potent anti apoptotic effect on quiescent cells , when TrkA was prevalent at the cell surface , showing that NGF can have a dual action on SH SY5Y cells depending on the relative cell surface expression of TrkA and P75NTR . ^^^ The pro apoptotic activity of NGF but not its anti apoptotic activity was abrogated by an antibody against the extracellular domain of P75NTR and in cell isolated from P75NTR knock out mice indicating that NGF exhibits a proapoptotic activity via P75NTR exclusively . ^^^ On the other hand , we showed that the anti apoptotic activity of NGF was specifically mediated by an interaction with TrkA with no contribution of P75NTR , as demonstrated on SK N BE cells transfected with TrkA in which NGF was a potent anti apoptotic compound but did not exhibit any pro apoptotic activity . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To understand the mechanism of neurotrophin stimulated calcium uptake , the regulation of calcium uptake and intracellular mobilization by nerve growth factor ( NGF ) was investigated using NIH 3T3 cells stably transfected with either the high affinity NGF receptor p 140 ( trk ) ( 3T3 Trk ) or the low affinity NGF receptor p 75 ( NGFR ) ( 3T3 p 75 ) . ^^^ Nifedipine , an inhibitor of calcium uptake through L type calcium channels , inhibited the action of NGF on both 3T3 Trk cells and 3T3 p 75 cells , indicating that both p 140 ( trk ) and p 75 ( NGFR ) receptors are linked to nifedipine sensitive L type calcium channels . ^^^ These studies show that either NGF receptor will support increases in intracellular calcium but that p 140 ( trk ) does so by increasing both uptake and mobilization , whereas p 75 ( NGFR ) does so by increasing uptake only . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
SH 2 B has been shown to be required for nerve growth factor ( NGF ) mediated neuronal differentiation and survival , associate with NGF receptor TrkA , and be tyrosyl phosphorylated in response to NGF . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Neurons surviving independent of NGF in macrowell culture were heterogeneous in size and were lacking the low affinity NGF receptor . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
During the initial hour of culture , the only detectable NGF receptor is p75NTR , which by comparative Western blot is expressed at 50 to 100 fold lower levels than on PC 12 cells . ^^^ An NGF mutant that is deficient in p75NTR binding has no effect on neuronal morphology or ceramide formation . ^^^ Furthermore , two anti p75NTR antibodies ( REX and 9651 ) , which are known to compete with NGF for binding to p75NTR , mimic the effects of NGF , whereas a monoclonal antibody ( MC 192 ) targeted against a different epitope does not . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The second gradient was apparent in the subplate and occurred in spatial opposition to a distinct gradient for the low affinity NGF receptor p 75 . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The ganglioside GM 1 is known to protect neuronal cell lines from apoptosis induced by serum / growth factor withdrawal and its effect is mediated in part by the direct activation of the trkA NGF receptor [ G . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The trkA proto oncogene encodes a high affinity NGF receptor that is essential for the survival , differentiation and maintenance of many neural and non neural cell types . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
While the physiological role of the low affinity NGF receptor ( p 75 ) has not been clearly defined , this receptor promotes activation of nuclear factor ( NF ) kappaB in Schwann cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To investigate the mechanisms by which Ras suppresses neuronal death , we examined whether Ras functions by inhibiting the proapoptotic p 53 pathway ( Jun N terminal kinase / p53 / BAX ) that is necessary for neuronal death after NGF withdrawal and p75NTR activation . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Human monocytes / macrophages ( M / M ) infected in vitro with HIV type 1 ( HIV 1 ) are able to produce substantial levels of NGF that are associated with enhanced expression of the high affinity NGF receptor ( p 140 trkA ) on the M / M surface . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Recent studies have demonstrated that nerve growth factor ( NGF ) induces apoptosis of several cell types in the central nervous system through its low affinity p 75 neurotrophin receptor ( p75NTR ) . ^^^ This pro apoptotic effect was completely reversed by the blocking anti p75NTR ( REX ) antibody which inhibits NGF binding to p75NTR . ^^^ Our results demonstrate , for the first time , that NGF can induce embryonic motoneuron apoptosis through its receptor p75NTR . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To define cellular mechanisms that modulate A ( 2A ) AR expression , A ( 2A ) AR mRNA and protein levels were examined in three PC 12 sublines : 1 ) PC12nnr5 cells , which lack the high affinity NGF receptor TrkA , 2 ) srcDN 2 cells , which overexpress kinase defective Src , and 3 ) 17 . 26 cells , which overexpress a dominant inhibitory Ras . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Recent studies have suggested that NGF receptor activation may occur in caveolae or caveolae like membranes ( CLM ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Binding of four mammalian neurotrophins , nerve growth factor ( NGF ) , brain derived neurotrophic factor ( BDNF ) , neurotrophin 3 ( NT 3 ) , and neurotrophin 4 / 5 ( NT 4 / 5 ) , to p75NTR is studied by molecular modeling based on 10 ray structures of the neurotrophins and the extracellular domain of p55TNFR , a homologue of p75NTR . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The neurotrophin receptor ( p75NTR ) is best known for mediating tropic support by participating in the formation of high affinity nerve growth factor ( NGF ) receptor complexes with trkA , however , p75NTR more recently has been shown to act as a bona fide death signaling receptor , which can signal independently of trkA . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Anandamide ( ANA ) inhibits prolactin and nerve growth factor ( NGF ) induced proliferation of human breast cancer cells by decreasing the levels of the 100 kDa prolactin receptor ( PRLr ) and the high affinity trk NGF receptor , respectively , and by acting via CB ( 1 ) like cannabinoid receptors . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To investigate further its possible mode of action , the ability of monoamine activated alpha1M and normal alpha1M to bind and to activate the NGF receptor ( TrkA ) was investigated . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Moreover , the results showed that the optic nerves also expressed trkA NGF receptor and NGFmRNA , most likely by oligodendrocytes , implying that NGF is locally produced and suggesting that the presence of NGF might be regulated by an autocrine mechanism . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The proliferative effect of NGF appeared to be mediated by the high affinity NGF receptor , p140trka . ^^^ Only p140trka , but not the low affinity NGF receptor , p75LNGFR , was expressed in LNCaP cells ; both the proliferative response and the phosphorylation of p140trka upon NGF treatment were prevented by the tyrosine kinase inhibitor K252a . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
While FGFR 1 interacts with FRS 2 constitutively , independent of ligand stimulation and tyrosine phosphorylation , NGF receptor ( TrkA ) binding to FRS 2 is strongly dependent on receptor activation . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Immunotoxic lesions targeting low affinity NGF receptor ( p75NGF receptor ) bearing CBF neurons provide a selective model for testing the effects of NGF on residual cholinergic neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
K252a , a selective inhibitor for NGF receptor gp140trk , had no effect on acrylamide induction , but completely inhibited NGF induced neurofilament protein synthesis . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
These results suggest that c Abl may be recruited to the NGF receptor complex and be involved in regulating specific phosphorylation events that occur during neuronal differentiation . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
While MC CM raised mRNA for trkA , the effect was not synergistic with NGF . mRNA for the common neurotrophin receptor ( p75NTR ) showed a modest synergistic increase . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Human keratinocytes also synthesize and express the low ( p 75 ) and the high affinity ( trk ) NGF receptor ( NGF R ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Injection of the steroid resulted in increased intraovarian synthesis of NGF and its low affinity receptor , p 75 NGFR . ^^^ Intraovarian injections of the retrograde tracer fluorogold combined with in situ hybridization to detect tyrosine hydroxylase ( TH ) messenger RNA containing neurons in the celiac ganglion revealed that these changes in NGF / p75 NGFR synthesis are accompanied by selective activation of noradrenergic neurons projecting to the ovary . ^^^ Intraovarian administration of a neutralizing antiserum to NGF in conjunction with an antisense oligodeoxynucleotide to p 75 NGFR , via Alzet osmotic minipumps , restored estrous cyclicity and ovulatory capacity in a majority of EV treated rats . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Neurotrophin dependence mediated by p75NTR : contrast between rescue by BDNF and NGF . ^^^ Expression of wild type p75NTR created a state of neurotrophin dependence : cells could be rescued by nerve growth factor ( NGF ) , brain derived neurotrophic factor ( BDNF ) , or neurotrophin 3 ( NT 3 ) , but not by a mutant NGF that binds well to Trk A but poorly to p75NTR . ^^^ Moreover , expression of mutant p75NTR ' s in another neurotrophin insensitive cell line ( HEK293T ) showed that a domain within the intracellular domain governs alternate responses to neurotrophins : the carboxy terminus of the intracellular domain of p75NTR including the sixth alpha helix domain is necessary for rescue by BDNF , but not NGF . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
However , during development precursors to rat sympathetic neurons in the thoracolumbar region are not responsive to NGF because they lack the signal transducing NGF receptor , trkA . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Depletion of nerve growth factor ( NGF ) from differentiated , neuronal PC 12 cells causes a form of programmed cell death that stems from the attenuation of NGF receptor signaling and the resultant expression of certain genes required for cell death . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The low affinity neurotrophin receptor p75NTR can mediate cell survival as well as cell death of neural cells by NGF and other neurotrophins . ^^^ Furthermore , p75NTR / NADE induced cell death was dependent on NGF but not BDNF , NT 3 , or NT 4 / 5 , and the recruitment of NADE to p75NTR ( intracellular domain ) was dose dependent . ^^^ Taken together , NADE is the first signaling adaptor molecule identified in the involvement of p75NTR mediated apoptosis induced by NGF , and it may play an important role in the pathogenesis of neurogenetic diseases . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Preceding the development of follicular cysts , there is an increase in intraovarian synthesis of nerve growth factor ( NGF ) and the low affinity NGF receptor ( p 75 NGFR ) . ^^^ Selective blockade of NGF actions and p 75 NGFR synthesis in the ovary restored estrous cyclicity and ovulatory capacity in estradiol valerate treated rats , suggesting that an increase in NGF dependent , p 75 NGFR mediated actions within the ovary contributes to the development of cystic ovarian disease . ^^^ The NGF producing cells , detected by their content of immunoreactive p 75 NGFR material , were found scattered throughout the ovary with some of them infiltrating the granulosa cell compartment of large , precystic follicles . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
TrkA , the NGF receptor tyrosine kinase , was expressed in the nodose ganglion throughout development and there was a marked decrease in TrkA mRNA expression in the nodose ganglion of NGF / embryos . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Only the TrkA and not the p 75 NGF receptor was detected at m RNA and protein level , and only the TrkA NGF receptor was up regulated during NGF driven culture . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To study peripheral neuron development in the absence of neurotrophin signaling , we have generated mice that are double null for BAX and nerve growth factor ( NGF ) , and BAX and the NGF receptor TrkA . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Proteins for both the high affinity NGF specific tyrosine kinase binding site ( p140TrkA ; TrkA ) and the low affinity neurotrophin ( p75NTR ) receptor were present in all three GBM cell lines . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Nerve growth factor ( NGF ) plays a crucial role in synaptic plasticity during brain development and adulthood by activating a dual receptor system composed of TrkA and p 75 ( p75NTR ) receptors . ^^^ The ability of glucocorticoids to increase NGF expression in the hippocampus prompted us to investigate whether the synthetic glucocorticoid dexamethasone ( DEX ) increases TrkA and p75NTR expression in NGF target cholinergic neurons in developing rats . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In conjunction , evidence of reduced sympathetic innervation in the failing heart was apparent , as measured histologically by catecholamine fluorescence and by expression of the neuronal NGF receptor trkA . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Wild type SH SY5Y cells are unresponsive to nerve growth factor ( NGF ) , but cells transfected with the high affinity NGF receptor TrkA ( SH SY5Y / TrkA ) differentiate in response to NGF . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The discoidin domain receptor ( DDR 1 ) is characterized by a discoidin 1 motif in the extracellular domain , an unusually long cytoplasmic juxtamembrane ( JM ) region , and a kinase domain that is 45 % identical to that of the NGF receptor , TrkA . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The authors demonstrate for the first time the immunocytochemical expression of the low affinity neurotrophic growth factor ( NGF ) receptor p75LNGFR and the high affinity NGF receptor trkA in the human testis . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Cholinergic medial septum neurons express TrkA and p 75 nerve growth factor receptor ( p 75 ( NGFR ) ) and interactions between TrkA and p 75 ( NGFR ) are necessary for high affinity binding and signaling of nerve growth factor ( NGF ) through TrkA . ^^^ In adult p 75 ( NGFR ) deficient ( / ) mice , retrograde transport of NGF and other neurotrophins by these neurons is greatly reduced , however , these neurons maintain their cholinergic phenotype and size . ^^^ Here , we investigated whether chronic and long term absence of p 75 ( NGFR ) ( and possibly reduced NGF transport and TrkA binding ) would affect the cholinergic septohippocampal system during aging in mice . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The peripheral expression of trkA encoding for NGF receptor was investigated by RNase protection assay . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To test this hypothesis , we measured NGF content and NGF receptor expression , p 75 ( NTR ) ( low affinity neurotrophin receptor ) and trkA ( high affinity NGF receptor ) , in control and diabetic rat SMG , CG and superior cervical ganglia ( SCG ) . ^^^ NGF receptor expression was not consistently altered in any of the autonomic ganglia . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Our results illustrate the importance of the genetic background and the motoneuron sub type in studies related to cell death and survival of motoneurons in relation to NGF and p75NTR . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
CONCLUSIONS : IFN gamma could induce the functional NGF receptor even in the aggressive phenotype of NB . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
These results indicate human neurofibroma cells possess functional NGFR and the growth is potentiated through the NGF NGFR system in the paracrine and / or autocrine fashion . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Cellular responses to NGF are facilitated by two receptors called trk A and p 75 neurotrophin receptor ( p75NTR ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In this study , we show that triggering of the high affinity NGF receptor TrkA with agonists protects monocytes from apoptosis induced by gliotoxin or UVB radiation . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In the present study , we assessed the temporal change in gene expression of neurotrophins , NGF , BDNF , and NT 3 , and their receptors , low affinity neurotrophin receptor ( p75NTR ) and Trks A , B , and C , by RT PCR technique in the liver of rats treated with lead nitrate ( LN ; 0 . 1 mmol / kg body weight ) , an inducer of liver hyperplasia . ^^^ The mRNAs for NGF , BDNF with exon 4 , NT 3 , p75NTR , and all Trk members were detected in the LN untreated liver . ^^^ LN treatment resulted in increases in the levels of NGF , BDNF with exon 4 , NT 3 , p75NTR , and TrkA mRNAs and further led to expression of BDNF mRNA with exon 3 . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Moreover , combined deep etching and immunogold techniques revealed the presence of the NGF receptor TrkA in the caveolae of PC 12 cells . ^^^ Taken together , our results demonstrate the presence of caveolae in PC 12 cells , which contain the high affinity NGF receptor TrkA , and the specific involvement of these cholesterol enriched plasma membrane microdomains in the propagation of the NGF induced signal . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NRAGE binds p75NTR in vitro and in vivo , and NRAGE associates with the plasma membrane when NGF is bound to p75NTR . ^^^ NRAGE blocks the physical association of p75NTR with TrkA , and , conversely , TrkA overexpression eliminates NRAGE mediated NGF dependent death , indicating that interactions of NRAGE or TrkA with p75NTR are functionally and physically exclusive . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Intracelluar dynamics of a high affinity NGF receptor TrkA in PC 12 cell . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The C D loop in nerve growth factor ( NGF ) is involved in binding to the NGF receptor , TrkA . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NGF also induced the expression of the p 75 NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Although NGF , brain derived neurotrophic factor ( BDNF ) , and neurotrophin 3 ( NT 3 ) accelerated catagen in the organ cultured skin of C57BL / 6 mice , these neurotrophins failed to promote catagen development in the organ cultured p75NTR null skin . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In the present study , the changes in expression of the neurotrophins ( NGF , BDNF , and NT 3 ) and their cognate receptors ( i . e . , trk and p75NTR ) during the progression of normal prostatic epithelial cells to malignancy were documented . ^^^ In contrast , malignant prostate epithelial cells directly secrete a series of neurotrophins ( i . e . , NGF , BDNF , and / or NT 3 ) and express at least one if not more of the trk receptor proteins ( i . e . , trk A , B , and / or C ) , while no longer expressing the p75NTR receptors . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In order to find out whether thymic epithelial cells are responsive to NGF during the whole lifespan of the rat , we studied NGF receptor expression in the thymus from birth to 2 years of age , using immunohistochemistry . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Impaired NGF production and release has been documented in aged animals , suggesting that decreased NGF receptor stimulation may be one factor contributing to neuronal dysfunction with aging . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
TRK T 1 oncogene is generated by the rearrangement of the NGF receptor TrkA with TPR . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Furthermore , antisense p75NTR oligonucleotides rescued trkA , B , and C expressing neurons , while NGF and leukemia inhibitory factor targeted primarily the trkA positive neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
PC 12 and INS 1 cells both express the nerve growth factor ( NGF ) receptors trkA and p75NTR and the epidermal growth factor receptor ( EGF ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
PS 1 protein accumulation was dose responsive to NGF and required the presence of the TrkA NGF receptor tyrosine kinase . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The immunotoxin 192 IgG saporin ( SAP ) provokes a selective loss of NGFr positive BFCN , somewhat similar to the loss of integrity of BFCN associated with human senile dementia of Alzheimer ' s type , whereas NGF exerts a trophic action on BFCN . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Using a quantitative image analyzer , morphometric and densitometric parameters of ChAT and p75NTR positive cells were measured immediately after cessation of NGF infusion . ^^^ NGF did not influence the morphology of p75NTR positive neurons , which were well labeled , irrespective of treatment and age of the rats . ^^^ In 4 month old rats , NGF infusion decreased the intensity of both ChAT and p75NTR immunostaining . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
METHODS : Immunocytochemistry and Western blot analyses were performed using primary antibodies raised against NTs ( nerve growth factor [ NGF ] , brain derived neurotrophic factor [ BDNF ] , NT 3 , and NT 4 / 5 ) and NT receptors ( TrkA , TrkB , TrkC , and p75NTR ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
CD 137 ( ILA / 4 1BB ) is a member of the TNF / NGF receptor family , and has previously been suggested to be involved in T cell activation and differentiation . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Thus , we concluded that high levels of female steroid hormones such as those due to pregnancy or hormonal replacement therapy could increase NGF receptor expression in DRG that carry more NGF to elevate the CGRP synthesis in these groups . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In this investigation , we assessed the neurochemical and structural plasticity of trigeminal sensory neurons in p75NTR deficient mice in response to either normal or elevated levels of NGF during postnatal development and into adulthood . ^^^ In response to elevated levels of NGF protein , transgenic mice with and without p75NTR expression display both increased levels of trkA mRNA expression and a greater density of trigeminal central afferent axons as compared to control mice . ^^^ These data further reveal that an absence of p75NTR function in trigeminal sensory neurons does not diminish their capacity for NGF dependent plasticity , namely trkA mRNA expression and collateral growth of central afferent axons . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The effect of NGF appeared to be mediated by its trkA receptors ( trkA ( NGFR ) ) , as suggested by the potent inhibition of both MCF 7 cell proliferation and trkA ( NGFR ) phosphorylation occurring upon treatment of cultures with the selective trkA ( NGFR ) inhibitor K252a . ^^^ Surprisingly , the antiestrogen drug tamoxifen ( TAM ) inhibited NGF induced MCF 7 cell proliferation and trkA ( NGFR ) phosphorylation in a concentration related fashion . ^^^ The effect of TAM seemed to be estrogen receptor independent , because the pure estrogen receptor antagonist ICI 182 . 780 was unable to block NGF induced trkA ( NGFR ) phosphorylation . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The TrkA NGF receptor extracellular region contains three leucine repeats flanked by cysteine clusters and two immunoglobulin like domains that are required for specific ligand binding . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Antibody to the extracellular domain of the low affinity NGF receptor stimulates p 75 ( NGFR ) mediated apoptosis in cultured sympathetic neurons . ^^^ Although cell death due to NGF withdrawal is a well described , apoptosis in sympathetic neurons through stimulation of p 75 ( NGFR ) has not been clearly demonstrated . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Using immunohistochemistry , we demonstrated that the mucosa is the primary source of NGF in the mouse bladder , and the bladder mucosa also expresses estrogen receptor ( ER ) alpha , ER beta , and the high affinity NGF receptor tyrosine kinase A . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In contrast , no significant changes in the expression of the NGF receptor TrkA were noted under the same conditions . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The increase of NGF is associated with enhanced expression of high affinity NGF receptor on M / M and with no changes of low affinity NGF receptors ( p 75 ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Nerve growth factor ( NGF ) regulates the nociceptive properties including sensitivity to capsaicin of a subset of dorsal root ganglion neurons , which express the high affinity NGF receptor , trkA . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Using a culture model of the endothelial blood brain barrier ( BBB ) , we found that NGF directly acts on blood derived monocytes via the p 75 NGF receptor , thus interfering with monocyte migration through the activated BBB endothelium . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Several lines of evidence implicate nerve growth factor ( NGF ) signaling through its receptors ( TrkA and p 75 ( NGFR ) ) as a potential source of communication between the two systems . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We have examined nerve growth factor ( NGF ) triggered signaling in two NIH3T3 cell lines exogenously expressing the NGF receptor , TrkA . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The mechanisms described explain at least in part why NGF binding to p75NTR increases cell survival in certain circumstances . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The contribution of low affinity NGF receptor ( p75NGFR ) to delayed neuronal death after ischemia in the gerbil hippocampus . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Sections immunostained for the p 75 NGF receptor ( p 75 ( NTR ) ) revealed circular patches scattered throughout the cortex and hippocampus of the APP ( swe ) / PS1 ( M146L ) mice that contained Abeta , were innervated by p 75 ( NTR ) ir neurites , but displayed virtually no immunopositive neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In general , the upregulation of NGF receptor immunoreactivities in astroglia correlated with the strong intensification of glial fibrillary acidic protein immunocytochemistry , a prominent feature of EAE . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
At E 17 , almost all Na ( 5 ) 1 . 8 expressing neurons also express the high affinity NGF receptor TrkA , and only a small proportion bind to IB 4 , a marker for c ret expressing ( glial derived neurotrophic factor responsive ) neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We measured local cerebral rates of glucose utilization ( ( 14 ) C 2 deoxyglucose ) throughout the brains of awake rats 4 . 5 months after bilateral intraventricular injections of a cholinotoxic antibody directed against the low affinity NGF receptor ( p 75 NGF ) associated with cholinergic neurons ( 192 IgG saporin ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Dorsal root ganglion ( DRG ) neurons that mediate nociception express the high affinity NGF receptor ( trkA ) gene and the preprotachykinin ( PPT ) gene . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To evaluate the ability of Zn2+ to attenuate the biological activities of NGF mediated by p75NTR , we characterized the effects of this transition metal cation on both binding and the pro apoptotic properties of the NGF p75NTR interaction . ^^^ Binding of NGF to p75NTR displayed higher affinity in embryonic chick retinal cells than in PC 12 cells . ^^^ The addition of 100 microM Zn2+ inhibited binding and chemical cross linking of 125I NGF to p75NTR , and also attenuated apoptosis mediated by this ligand receptor interaction . ^^^ These studies lead to the conclusion that Zn2+ antagonizes NGF / p75NTR mediated signaling , suggesting that the effect of this transition metal cation can be either pro or anti apoptotic depending on the cellular context . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Almost all BDNF mRNA expressing neurons coexpressed trkA , a high affinity NGF receptor , mRNA . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Mice carrying a mutation in the low affinity NGF receptor ( NGFR ) gene possess deficits in sensory innervation . ^^^ CCSP NGF transgenic mice exhibited a twofold increase in the number of lung lavage neutrophil level whereas NGFR knockout mice exhibited a nearly 50 % decrease in neutrophilic inflammation compared with wild type mice 18 h after ozone inhalation . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In this study we attempted to antagonize NGF by using trkA IgG , an inhibitor of NGF , consisting of the NGF receptor linked to an immunoglobulin . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
This effect is mediated by the activation of the NGF receptor TrkA because the specific inhibitor of Trk phosphorylation K252a also blocks NGF induced increase in insulin secretion , both in the presence and absence of exogenous NGF . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Developmental sympathetic neuron death is determined by functional interactions between the TrkA / NGF receptor and the p 75 neurotrophin receptor ( p75NTR ) . ^^^ Specifically , experiments presented here demonstrate that the presence or absence of p75NTR does not alter Trk activity or NGF and NT 3 mediated downstream survival signaling in primary neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Importantly , TeNT HC and NGF share the same retrograde transport organelles , which are characterized by the presence of the neurotrophin receptor p75NTR . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The finding that two proteins , p75NTR and Trk , act as receptors for NGF in neurons generated the discovery of other neurotrophic factors / receptor families and has enhanced our understanding of the development , survival , regeneration , and degeneration of the nervous system . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NGF receptor expression was determined by 2 color flow cytometry . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Sections of pancreatic cancer and normal pancreatic tissue were examined by immunohistochemistry for the expression of the neurotrophins NGF , BDNF , NT 3 , NT 4 , and their receptors TrkA , TrkB , and TrkC , as well as the low affinity receptor , p75NTR . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Sciatic nerve mRNA expression of IGF 1 , IGF 1 receptor , NGF , and p 75 ( low affinity NGF receptor ) , as well as protein expression of C FOS , were examined at various time points following crush injury and compared with age and sex matched nondiabetic BB / Wor rats . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The aim of the present study was to determine the NGF levels and the distribution of NGF and low affinity NGF receptor ( p75NGFR ) and mast cells ( MC ) in human atherosclerotic coronary arteries . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Ras independent pathways , originating from the NGF receptor , can thus synergize with second messenger analogues bypassing the ERK cascade but leading to the same biological result neurite formation . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
A slight transient increase in the expression of p 75 ( NGFR ) mRNA was observed by 2 to 4 h after retinoid treatment of undifferentiated cells , whereas a larger increase in the expression of both TrkA and p 75 ( NGFR ) mRNA up to threefold the basal level , was observed by 2 to 6 h after retinoid treatment of NGF differentiated cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To further examine this assertion in vivo , we have studied two populations of NGF dependent neurones during the period of naturally occurring neuronal death in mice that lack BAX , NGF or the NGF receptor TrkA , alone and in combination . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
These findings indicate that the ERK and JNK pathways collaborate downstream of the NGF receptor for regulation of the NFLC promoter . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Here we show that inactivation of p 75 ( NGFR ) , but not of trkA , resulted in D ( 2 ) receptor loss in responder cells and prevented D ( 2 ) receptor expression induced by NGF in the nonresponder . ^^^ Moreover , NGF treatment of nonresponder cells induced both nuclear translocation and DNA binding activity of NF kappaB complexes containing p 50 , p65 / RelA , and cRel subunits , an effect prevented by anti p 75 ( NGFR ) antibodies . ^^^ These data indicate that in prolactinoma cells the effect of NGF on D ( 2 ) receptor expression is mediated by p 75 ( NGFR ) in a trkA independent way and that NGF stimulation of p 75 ( NGFR ) activates NF kappaB , which is required for D ( 2 ) gene expression . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In the analysis of possible pathways involved in the regulation of NGFR expression , we found that neither blockade of the visual input nor NGF administration to the visual cortex resulted in a modulation of NGFR levels of expression . ^^^ In contrast , the expression level of the low affinity NGF receptor p 75 ( NTR ) seems to remain nearly constant throughout development . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In view of conflicting opinions on the identity of glial targets of NGF in human central nervous system ( CNS ) , we examined the cellular distribution of both NGF receptor subunits in normal CNS and in chronic multiple sclerosis ( MS ) lesions . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Nerve growth factor ( NGF ) can induce apoptosis in neural cells via activation of the low affinity neurotrophin receptor p75NTR . ^^^ NADE ( p75NTR associated cell death executor ) is a p75NTR associated protein that mediates apoptosis in response to NGF by interacting with the death domain of p75NTR in 293T , PC 12 , and nnr 5 cells ( Mukai , J . , Hachiya , T . , Shoji Hoshino , S . , Kimura , M . ^^^ Mutation of the NES also abolished self association of NADE , its interaction with p75NTR , and NGF dependent apoptosis . ^^^ Expression of a fragment of NADE ( amino acid residues 81 124 ) blocked NGF induced apoptosis in oligodendrocytes , suggesting that this region has a dominant negative effect on NGF / p75NTR induced apoptosis . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The NGF receptor trkA is a tyrosine kinase receptor comprising an extracellular domain with a ligand binding site , a transmembrane spanning domain ( TMD ) , and an intracellular domain composed of a juxtamembrane region ( JMR ) , a tyrosine kinase domain , and a short carboxy terminal tail . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
CONCLUSION : Restoration of high affinity NGF receptor ( trkA ) expression in neuroblastoma cells could induce non reversal differentiation . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
By NGF and p 75 NGF receptor double labelling , both immunoreactivities showed weak staining in the epidermis and dermis of normal skin . ^^^ However , in the dermis of prurigo nodularis , strong staining for both NGF and NGF receptor antibodies was seen . ^^^ NGF receptor immunoreactive nerves were more dense in areas where there were more NGF immunoreactive cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Inhibitors of phosphatidylinositol 3 kinase ( LY 294002 ) , mammalian target of rapamycin ( rapamycin ) , and the NGF receptor , TrkA ( K 252a ) , but not of mitogen activated protein kinase ( PD 98059 ) , prevented the recruitment of eEF1A 1 mRNA to polyribosomes . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The p 75 low affinity neurotrophin receptor ( p 75 ( LNTR ) ) appears to have various functions that include enhancing nerve growth factor ( NGF ) mediated survival by increasing TrkA ( high affinity NGF receptor ) efficiency , and mediating apoptosis by acting as a ligand regulated pro apoptotic receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
This response reached maximal levels as early as 5 min after the initiation of the coincubation and was completely blocked by anti NGF Ab or by an inhibitor for a tyrosine kinase of the trkA NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
METHODS : Expression of NGF , TrkA , and p75NTR was investigated in HCCs developing and regenerating livers , and primary hepatocyte cultures in B6C3F ( 1 ) mice by reverse transcription polymerase chain reaction , Northern blotting , and / or immunohistochemistry . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In addition , the effect of albumin seemed to depend on the activity of the NGF receptor , TrkA , suggesting that the glutamate synthesized and released due to the presence of albumin promotes neuronal survival through the activity of TrkA . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
PURPOSE : To determine the presence of nerve growth factor ( NGF ) , NGF mRNA , and NGF receptor ( TrkA ) in rabbit ocular tissues , and whether changes occur in NGF and NGF mRNA levels after experimental iridectomy . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The adjacent nerves expressed the high affinity NGF receptor trk A . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In vernal keratoconjunctivitis , NGF plasma levels correlate with the number of mast cells infiltrating the conjunctiva , and NGF mRNA is increased in nasal mucosal scrapings of patients with allergic rhinitis who have high levels of NGF in serum and nasal fluids ; NGF is further increased in nasal fluids after specific allergen challenge . ( 2 ) NGF is produced and released by several modulatory and effector cells of allergic inflammation and asthma , for example T helper 2 lymphocytes , mast cells and eosinophils . ( 3 ) NGF receptors are expressed on the conjunctival epithelium of patients with allergic conjunctivitis and the number of NGF receptor positive cells is increased in the conjunctiva of these patients . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
High affinity NGF receptor ( TrkA ) levels are decreased in AD brains as compared to age matched control brains . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The low affinity NGF receptor , p 75 , has long been thought merely to facilitate NGF binding to TrkA . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Low and high affinity NGF receptor immunoreactivity is markedly expressed during regeneration and at different stages of development in the mouse olfactory system . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The expression level of TrkA in PC 84 cells was comparable to that in PC 12 cells ; whereas that of another NGF receptor , p 75 , was significantly lower . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In cell lines derived from small cell lung cancer ( SCLC ) , NGF treatment stimulates NGF receptor expression , activates NGF secretion , inhibits proliferation and abrogates invasion . ^^^ Cell proliferation was measured by the [ ( 3 ) H ] thymidine incorporation test and NGF receptor expression was evaluated by immunofluorescence . ^^^ RESULTS : We found that RA increased the expression of both trkA and p 75 NGF receptors in NCI N 592 and GLC 8 cell lines and prevented the loss of both NGF production and NGF receptor expression occurring when NGF treatment was discontinued . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
METHODS : DU 145 cells were treated with NGF , then ARs and NGF receptor p 75 ( NGFR ) expression and telomerase activity were studied . ^^^ RESULTS AND CONCLUSIONS : NGF treatment induced a reversion of DU 145 cells to a less malignant phenotype , characterized by the re expression of ARs and p 75 ( NGFR ) NGF receptors . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The NGF receptor TrkA was found on all examined tumor cell lines and is not restricted to cells belonging to the nervous system . ^^^ Detection of NGF receptors TrkA and p75NTR in human tumor cell lines and effect of NGF on the growth characteristic of the UT 7 / EPO cell line . ^^^ The receptors for nerve growth factor ( NGF ) TrkA and p75NTR were detected at the mRNA and the protein level in various human tumor cell lines . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Rat visual cortical neurones express TrkA NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
RT PCR analysis and immunological methods demonstrate that human umbilical vein endothelial cells ( HUVECs ) express the NGF receptors trkA ( NGFR ) and p75NTR . ^^^ NGF treatment caused a rapid phosphorylation of trkA ( NGFR ) in HUVECs , determining a parallel increase of phosphorylated ERK1 / 2 . ^^^ Accordingly , NGF induced a significant increase in HUVEC proliferation that was abolished by the trkA ( NGFR ) inhibitor K252a . ^^^ Endogenous NGF was responsible for the basal levels of trkA ( NGFR ) and ERK1 / 2 phosphorylation observed in untreated HUVEC cultures . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Pharmacological and dominant negative inhibition and kinase assays indicate that Bgamma promotes neuritogenesis by stimulating the MAP kinase cascade downstream of the TrkA NGF receptor but upstream or at the level of the B Raf kinase . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
It occurs also in Panc 1 , but not in human mesangial cells . cAMP induced tyrosine phosphorylation of the NGF receptor is slower and correlates with Akt activation . ^^^ Inhibition of EGF receptor tyrosine phosphorylation , but not of the NGF receptor , reduces cAMP induced neurite outgrowth . ^^^ Together , our results demonstrate that cAMP induces dual signaling in PC 12 cells : transactivation of the EGF receptor triggering the ERK1 / 2 pathway and neurite outgrowth ; and transactivation of the NGF receptor promoting Akt activation and thereby modulating ERK1 / 2 activation and neurite outgrowth . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We examined the effects of NGF treatment on the growth potential of C 6 glioma , which expressed NGF receptor in vivo . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Precursors of NGF have been shown to be the predominant forms of this neurotrophin in human brain and peripheral tissues , and proNGF has been shown recently to preferentially bind p75NTR with high affinity . ^^^ Prepro NGF antibody immunostained rat large / medium sensory neurons , whereas only small sensory neurons were stained with antibodies to mature rhNGF , suggesting that prepro NGF may be preferentially taken up and transported by p75NTR . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Adrenergic mouse pheochromocytoma ( MPC ) cells from heterozygous neurofibromatosis knockout mice show little or no expression of the NGF receptor trk A and do not undergo neuronal differentiation in response to NGF . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
On the one hand , p75NTR provides a positive modulatory influence on nerve growth factor ( NGF ) signaling through the high affinity neurotrophin receptor TrkA , and hence increases NGF survival signaling . ^^^ In mouse hippocampal cells lacking p75NTR ( p75NTR / ) activation of TrkA by NGF was not detectable . ^^^ Moreover , neuroprotection by NGF against glutamate toxicity was abolished in p75NTR / neurons , and the expression of bcl 2 and bcl xl was markedly reduced as compared to wildtype cells . ^^^ P75NTR signaling independent of TrkA , such as increased neutral sphingomyelinase ( NSMase ) activity causing enhanced levels of ceramide , were not detected after exposure of hippocampal neurons to NGF . ^^^ Overall , our results indicate an essential role for p75NTR in supporting NGF triggered TrkA signaling pathways mediating neuronal survival in hippocampal neurons . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Increased expression of nerve growth factor ( NGF ) and high affinity NGF receptor ( p 140 TrkA ) in human osteoarthritic chondrocytes . ^^^ OBJECTIVE : We aimed to investigate the expression of nerve growth factor ( NGF ) and high affinity NGF receptor ( p 140 TrkA ) on chondrocytes from human healthy and osteoarthritic cartilage . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In the older GK rats , both mRNA and protein expression of nerve growth factor ( NGF ) in the sciatic nerve were significantly reduced ( p < 0 . 001 and p < 0 . 05 ) , and NGFR TrkA ( high affinity NGF receptor ) and NGFRp 75 ( low affinity NGF receptor ) protein expression was reduced in dorsal root ganglia ( DRG ) ( both p < 0 . 05 ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Biopsy specimens were taken at 3 , 5 and 9 days following treatment and immunohistochemistry method was used to detect the epidermal growth factor ( EGF ) , EGF receptor ( EGF R ) , NGF , NGF receptor ( NGF R ) , NGF , NGF R , CD 68 and CD 3 . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NTR in the spleen : age dependent changes , effect of NGF and 4 methylcatechol treatment , and structural changes in p75NTR deficient mice . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Short term treatment of PC 12 Fes cells with NGF enhanced tyrosine phosphorylation of Fes , suggesting upstream regulation by the NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Two classes of astrocytes in the adult human and pig retina in terms of their expression of high affinity NGF receptor ( TrkA ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The aim of the present study was to localize the neurotrophic factors glial cell line derived neurotrophic factor ( GDNF ) , nerve growth factor receptor ( NGFr ) and low sensitive receptor for NGF ( p75LNGFR ) in rat testes during the postnatal development . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In the NGF rich environment , p75NTR was re expressed on odontoblasts and the transcription factor NF kappa B activated . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In contrast , expression of the NGF receptor tyrosine kinase and of the SCF and GM CSF receptors was unchanged . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Safety of retroviral gene marking with a truncated NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
RESULTS : These results revealed a low amount of NGF in the plasma of old subjects associated with a reduced expression of mRNA for NGF receptor trkA in peripheral lymphocytes . ^^^ CONCLUSION : Our data indicate that peripheral lymphocytes are targets for circulating NGF and suggest that changes in NGF receptor expression on lymphocytes might be potential marker for aging lymphocytes . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The results suggest two unique functions for the high affinity heteromeric NGF receptor site : ( a ) integration of both the MAPK and Akt pathways in the production of NGF induced neurite outgrowth , and ( b ) rapid and sustained activation of the Akt pathway , with consequent long term cellular survival . ^^^ To address this issue , various combinations of transfected PC 12 cells expressing a platelet derived growth factor receptor TrkA chimera and the p75NTR selective nerve growth factor mutant ( Delta9 / 13 NGF ) were utilized to selectively stimulate TrkA or p75NTR signaling , respectively . ^^^ In addition , a new interaction in the TrkA / p75NTR heteromeric receptor signal transduction network was revealed , namely that NGF induced activation of the MAPK pathway appears to inhibit the parallel NGF induced Akt pathway . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Generally , NGF specifically recognizes neurons with the NGF receptor , whereas GFAP does the glia , and CNTF does the motor neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Here we show that survival of sympathetic neurons supported by NGF on distal axons requires the kinase activity of the NGF receptor , TrkA , in both distal axons and cell bodies . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Our data implicate the involvement of NGF receptors in the postischemic regulation of astrocytic function ; however , the lack of NGF receptor expression on some astrocytes suggests heterogeneity of astroglia population . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The p 75 neurotrophin receptor ( p75NTR ) is a member of the tumor necrosis factor receptor ( TNFR ) superfamily , and can mediate both cell survival and cell death in response to nerve growth factor ( NGF ) . ^^^ NADE ( p75NTR associated cell death executor ) is a p75NTR associated protein that mediates apoptosis in response to NGF by interacting with the cell death domain of p75NTR . ^^^ In this review , we focus on the proapoptotic actions of NADE that regulate p75NTR signaling in response to NGF . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
METHODS : Retroviral vectors were constructed to express the high affinity NGF receptor ( TrkA ) and low affinity NGF receptor ( p 75 ( NTR ) ) . ^^^ CONCLUSIONS : The results indicate that coexpression of both low and high affinity NGF receptors are not only more efficient in restoration of NGF induced differentiation pathway , but also be able to activate the pro apoptotic activity of low affinity NGF receptor and make the tumor cells become NGF dependent and irreversibly differentiated . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
However , NGF rescue from growth factor withdrawal requires NGF signalling through the high affinity tyrosine kinase receptor ( TrkA ) , while NGF rescue from TNFalpha promoted cell death requires NGF signalling through the low affinity p75NTR receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Disruption of the phosphoinositide 3 kinase ( PI 3 kinase ) pathway , one of the signaling pathways downstream from NGF receptor binding , completely eliminates NGF effects on mitochondrial behavior in axons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Pathways responsible for transducing the sensitisation of TRPV 1 by TrkA , the NGF receptor , were characterised by observing the effects of inhibitors of key members of NGF activated second messenger signalling cascades . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
High affinity NGF receptor in the rat spinal cord during acute and chronic phases of experimental autoimmune encephalomyelitis : a possible functional significance . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
METHODS : Rat lenses were dissected and the expression of NGF , NGF mRNA and high affinity NGF receptor ( TrkA ) was evaluated by immunohistochemistry , immunoenzymatic assay ( ELISA ) and in situ hybridization ( ISH ) techniques . ^^^ RESULTS : The epithelium from fresh rat lenses expresses NGF receptor , NGF protein and NGF mRNA . ^^^ Moreover , immunohistochemistry shows that lens epithelial cells also express the NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Finally , levels of TrkA NGF receptor in sensory neurons of T 13 and L 1 dorsal root ganglia ( DRG ) , which supply the uterus , were assessed using densitometric immunohistochemistry . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
This study examined the roles of nerve growth factor ( NGF ) and the p 75 neurotrophin receptor ( p75NTR ) in the growth of dorsal root ganglion ( DRG ) central processes in the dorsal horn . ^^^ Two genetically modified mouse strains were used : transgenic mice that overexpress NGF in the CNS under the control of the glial fibrillary acidic protein promoter , and p75NTR exon 3 null mutant mice that express a hypomorphic form of this receptor . ^^^ In both NGF transgenic and nontransgenic mice with hypomorphic expression of p75NTR , there is a significant loss of DRG neurons compared to mice with normal p75NTR expression . ^^^ Comparisons of calcitonin gene related peptide immunoreactivity in the dorsal horn revealed that the area occupied by DRG central processes was not significantly different between p75NTR hypomorphic mice and wild type siblings , or between NGF transgenic mice with either hypomorphic or normal expression of p75NTR . ^^^ We propose that DRG central processes arborize extensively in both NGF transgenic and nontransgenic p75NTR hypomorphic mice in order to compensate for the loss of DRG neurons and restore dorsal horn innervation to normal levels . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Quantitative in situ hybridization was used to detect changes in neuronal PACAP mRNA expression and to correlate this expression with neuronal mRNA expression of the NGF receptor tyrosine kinase ( trk ) A . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Previous studies have shown that the activation kinetics of the NGF receptor , TrkA , and downstream protein kinases appear rapid and seemingly transient after NGF treatment of naive PC 12 cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Importantly , the constitutive growth of breast cancer cells was strongly inhibited by either NGF neutralizing antibodies or K 252a , a pharmacological inhibitor of NGF receptor TrkA , indicating the existence of an NGF autocrine loop . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Recent evidence indicates that some thymic cells of developing and adult laboratory animals express the neurotrophin NGF and its low affinity p75NTR and high affinity TrkA receptor . ^^^ The immunoreactivity of NGF , p75NTR , TrkA and cytokeratin was found in the epithelial thymocyte microenvironment . ^^^ They suggest a possible immunotrophic / immunoregulatory role of the NGF p75NTR TrkA system for T cell development in human thymus during senile involution . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In B5P cells , a clonal cell line of PC 12 cells overexpressing tyrosine kinase NGF receptor ( TrkA ) , cPA elicited transphosphorylation of TrkA . cPA elicited ERK activation was blocked by K252a and PKI . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Previously , we have shown using the double immunostaining method that the NGF receptors ( NGFR ) p 75 and TrkA are expressed mainly on subpopulations of GFAP+ astrocytes , and this expression increases progressively after ischemia . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In addition , we found that TrkA , the high affinity NGF receptor , was only found on fibers projecting into the barrel cortex . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Postganglionic sympathetic neurons , which are exquisitely sensitive to small changes in levels of target derived nerve growth factor ( NGF ) , express two transmembrane receptors : 1 ) the trkA receptor mediates neuron survival and neurite outgrowth ; and 2 ) the p 75 neurotrophin receptor ( p75NTR ) enhances neuronal responsiveness of trkA to NGF . ^^^ Elevating levels of NGF induces several morphological and neurochemical alterations in sympathetic neurons , including axonal sprouting , increased levels of p75NTR mRNA relative to trkA mRNA , and increased accumulations of NGF in hypertrophied somata . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Inactivation of trkA , but not of p75NTR , and wortmannin prevented NGF induced p 53 nuclear translocation . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Recent findings indicate an early defect in NGF receptor expression in CBF neurons ; therefore treatments aimed at facilitating NGF actions may prove highly beneficial in counteracting the cholinergic dysfunction found in end stage AD and attenuating the rate of degeneration of these cholinergic neurons . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
An 11 mer antisense PNA directed at the initiation codon , but not downstream gene sequences , dose dependently inhibited p75NTR expression and death signalling by nerve growth factor ( NGF ) in Schwann cell cultures . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NGF protein levels in the culture medium , NGFmRNA and high affinity NGF receptor ( Tyrosine kinase A : TrkA ) expression in the cells were evaluated in basal conditions and after stimulation with pro inflammatory cytokines or with the neuropeptide cholecystokinin 8 ( CCK 8 ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We found that in sympathetic neurons , selective activation of p75NTR by brain derived neurotrophin factor or NGF plus K252a induces elevation of ceramide that correlates with SM hydrolysis . ^^^ However , p75NTR activation does not generate sufficient ceramide to block apoptosis probably due to the rapid decrease in p75NTR expression that occurs upon NGF withdrawal . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
PC 12 cells express two types of membrane receptor for NGF : TrkA receptors and p75NTR receptors , and it was not clear from our studies which receptor was responsible . ^^^ Similarly , NGF fails to activate GSK3beta phosphorylation of MAP1B in PC 12 cells that lack TrkA receptors but express p75NTR receptors ( PC 12 nnr ) . ^^^ Chick ciliary ganglion neurons in culture lack TrkA receptors but express p75NTR and also fail to show NGF dependent GSK3beta phosphorylation of MAP1B , whereas in rat superior cervical ganglion neurons in culture , NGF activation of TrkA receptors elicits GSK3beta phosphorylation of MAP1B . ^^^ These results suggest that the activation of GSK3beta by NGF is mediated through the TrkA tyrosine kinase receptor and not through p75NTR receptors . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Here , we have tested whether overexpression of p75NTR could modulate survival of sympathetic neurons cultured in the presence or absence of NGF . ^^^ We found that over expression of p75NTR had no effect on the survival of sympathetic neurons cultured in the presence of NGF but instead delayed neuronal death following NGF deprivation . ^^^ These results strongly support the finding that p75NTR is not involved in the apoptosis process induced by NGF deprivation in sympathetic neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The local amount of NGF , the immunolocalization of p 75 neurotrophin receptor ( p75NTR ) and the number of MC were correlatively examined in coronary vascular wall and in the surrounding subepicardial adipose tissue , obtained from autopsy cases in humans with advanced coronary atherosclerosis . ^^^ The results demonstrate that NGF levels are decreased in atherosclerotic coronary vascular tissue but increased in the subepicardial adipose tissue , whereas both tissues express a greater number of MC and a stronger p75NTR immunoreactivity , compared to controls . ^^^ In effect , we provide the first evidence for ( 1 ) an altered presence of NGF , p75NTR and MC in both coronary vascular and subepicardial adipose tissue in human coronary atherosclerosis , and ( 2 ) a significant decrease in plasma NGF and BDNF levels and an elevated amount of plasma leptin and adipose MC in metabolic syndrome patients . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In immunohistochemical studies , we found that keratinocytes in lesional and nonlesional psoriatic tissue express high levels of nerve growth factor ( NGF ) and that there is a marked upregulation of NGF receptors , p 75 neurotrophin receptor ( p75NTR ) and tyrosine kinase A ( TrkA ) , in the terminal cutaneous nerves of psoriatic lesions . ^^^ These observations , as well as recent findings about NGF induced chemokine expression in keratinocytes , further substantiate a role of the NGF p75NTR TrkA system in the inflammatory process of psoriasis . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In addition to the Trks , the neurotrophins all bind to another cell surface receptor ( known as p 75 or the low affinity NGF receptor ) , whose role remains quite controversial . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In both systems , high dose NGF inhibited neurite outgrowth , implying that p75NTR is not involved in suppression of neurite outgrowth . ^^^ High dose NGF may induce cytoplasmic relocation of the receptor TrkA and axonal growth arrest independently of p75NTR . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In PC 12 cells , it activated the high affinity NGF receptor , TrkA , and displayed NGF like bioactivity by promoting neurite outgrowth and cell survival after serum deprivation . ^^^ Furthermore , the peptide enhanced gene expression in NGF receptor rich rat primary cortex neurons but not glial cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The expression of pPRAS , pPRAS / pAkt , and pPRAS / 14 3 3 increased in NGF treated mice but decreased with inhibition of phosphatidylinositol 3 kinase and the NGF receptor after tFCI . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
For example , murine hair follicles ( HF ) show developmentally and spatio temporally stringently controlled expression of NTs , including nerve growth factor ( NGF ) , brain derived neurotrophic factor ( BDNF ) , neurotrophin 3 ( NT 3 ) , and NT 4 , and their cognate receptors , tyrosine kinase A C ( TrkA C ) and p 75 neurotrophin receptor ( p75NTR ) . ^^^ NT 3 / TrkC and NGF / TrkA signaling stimulate HF development , while NT 3 , NT 4 and BDNF inhibit the growth ( anagen ) of mature HF by the induction of apoptosis driven HF regression ( catagen ) . p75NTR stimulation inhibits HF development and stimulates catagen . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
AEC expressed both high affinity ( TrkA ) and low affinity ( p 75 ( NGFR ) ) receptors for NGF . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Moreover , similar to previous observations in rat , the high affinity NGF receptor TrkA is also expressed in beta cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Functional role ( s ) for the common neurotrophin receptor p75NTR in nerve growth factor ( NGF ) signaling have yet to be fully elucidated . ^^^ In the present study , we have examined the influence of p75NTR on NGF mediated protective effects from hydrogen peroxide ( H2O2 ) induced apoptotic cell death of PC 12 cells . ^^^ When NGF binding to p75NTR was blocked by treating cells with either BDNF or PD 90780 , and where p75NTR expression was reduced by treating cells with antisense oligonucleotide to p75NTR , the protective effects of NGF were attenuated . ^^^ Further , NGF had no effect on cell viability in PC12nn5 cells , which express only p75NTR . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We show here that their respective receptors , TrkA , TrkB , TrkC , as well as p 75 ( NTR ) ( the low affinity NGF receptor ) , are expressed in vitro by the nasal ensheathing cells ; the three neurotrophins promote purification and proliferation of these glial cells , with an optimal concentration of 50 ng / ml ; and human ensheathing cells can be easily biopsied and highly purified using a serum free medium supplemented with NT 3 . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
CONCLUSION : The data indicate that p75NTR may be the main NGF receptor in the myocardium , and p75NTR immunopositive interstitial cells may have a role in regulating sympathetic nerve growth in canine heart . . ^^^ However , it is unclear whether the NGF high affinity receptor tyrosine kinase A ( TrkA ) and the NGF low affinity receptor p75NTR are altered in the state of sympathetic hyperinnervation in the heart . ^^^ The density of p75NTR immunolabeled myocardial interstitial cells at the NGF infusion site was lower than that at the site remote from NGF infusion , yet the sympathetic nerve density was higher at the infusion site than the remote area . ^^^ The density of p75NTR also was lower in the myocardium with high sympathetic nerve density , induced by NGF infusion or chronic electric stimulation of the left stellate ganglia , compared to control groups . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The p 75 NGF receptor exposed . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Human melanoma cell lines express low affinity NT receptor p75NTR in relation to their brain metastatic propensity with their invasive properties being regulated by NGF , or nerve growth factor , the prototypic NT . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Gene expression of NGF and its high affinity receptor ( TrkA ) are reduced in tumour tissues , whereas the number of cells immunopositive to the low affinity NGF receptor ( p 75 ) is increased . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NGF regulates cell survival and cell death via binding to two different receptors , TrkA and p75NTR ( ref . 10 ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NGF increased proliferation of Neuro 2a cells , which express p75NTR but no TrkA receptors on their surface . ^^^ On the other hand , SK N BE cell proliferation was decreased after NGF treatment , even though these cells also express p75NTR but no TrkA receptors on their surface . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Nerve growth factor ( NGF ) functions as a ligand for two receptors , the TrkA tyrosine kinase receptor and the p 75 neurotrophin receptor ( p75NTR ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The pharmacological inhibition of phosphatidylinositol 3 kinase ( PI3K ) by wortmannin significantly suppressed the survival supporting activity of MC 14 , whereas the NGF receptor ( tyrosine kinase A or TrkA ) inhibitor K252a showed no detectable effect on MC 14 activity . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The objective of the present report was to study the expression of the low affinity nerve growth factor ( NGF ) receptor p 75 and of the activated high affinity NGF receptor TrkA in malignant mesothelioma ( MM ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
There are increased levels of NGF in the keratinocytes and upregulation of NGF receptor ( NGF R ) in the cutaneous nerves of psoriatic plaques . ^^^ The transplanted psoriatic plaques on the SCID mice ( n=12 ) were treated with K252a , a high affinity NGF receptor blocker . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Autophosphorylation of NGF receptor TrkA and activation of ERK1 / 2 after NGF treatment were scarcely detected in GM1+ cells . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
This study examined the effects of hypomorphic p 75 neurotrophin receptor ( p75NTR ) expression and high levels of nerve growth factor ( NGF ) on trkA phosphorylation and downstream activation of p44 / 42 mitogen activated protein kinase ( MAPK ) . ^^^ Post ganglionic sympathetic neurons from postnatal day 1 p75NTR exon 3 null mutant ( p 75 ( / ) ) and 129 / SvJ mice were cultured in the presence of 50 ng / mL NGF and analysed by Western blotting . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
These endothelial cells also express p75NTR and respond to its preferred ligand , pro nerve growth factor ( pro NGF ) , by undergoing apoptosis . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
At necroscopy , adductors were removed for quantification of microvessel density , endothelial cell apoptosis and NGF receptor expression . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Compared to TrkA , the function of p75NTR in nerve growth factor ( NGF ) endocytosis is less clear , and it is unknown whether p75NTR by itself may internalize other neurotrophins besides NGF . ^^^ We directly compared TrkA and p75NTR for their ability to internalize NGF , and we also examined the endocytosis of iodinated brain derived neurotrophic factor ( BDNF ) and neurotrophin 3 ( NT 3 ) by p75NTR . ^^^ Cells expressing solely TrkA internalized NGF more efficiently than cells expressing p75NTR . ^^^ Surprisingly , cells expressing only p75NTR internalized far more BDNF or NT 3 than NGF . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
By real time PCR and immunohistochemistry , stress exposed mice showed an up regulation of NGF and its low affinity receptor p75NTR ; the NGF high affinity receptor TrkA was moderately down regulated . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In these mice , cells expressing cholinergic neuron markers , such as choline acetyltransferase , vesicular acetylcholine transporter and p 75 low affinity NGF receptor , were markedly reduced in the basal forebrain , whereas other cholinergic neurons including brain stem and spinal motor neurons expressed the markers . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The induction of neurite outgrowth by this NGF has been found to involve the high affinity trkA p75NTR complex of receptors . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We have now detected , by immunohistochemistry , beta 3 subunits in 82 % of small / medium and 67 % of large diameter sensory neurons in intact human dorsal root ganglia : 54 % of beta 3 small / medium neurons were NGF receptor trkA negative . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
This interest is based on the observation that CBF neurons , which provide the major source of cholinergic innervation to the cerebral cortex and hippocampus , undergo selective and severe degeneration in advanced AD and that the survival of CBF neurons depends upon NGF and its receptors , namely , trkA and p75NTR . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Persisting embryonal neural crest cells underwent further changes , such as MYCN amplification and repression of NGF receptor expression , during tumor progression . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
However , information is still scarce about the role of the cellular localization of the TrkA co receptor , p75NTR ( where NTR is neurotrophin receptor ) , following stimulation by NGF . ^^^ We found that , after binding to NGF , p75NTR was co immunoprecipitated with TrkA and was transcytosed at the basolateral membrane . ^^^ Interestingly , TrkA specific stimulation by basolateral NGF loading also induced the TrkA p75NTR interaction and subsequent p75NTR transcytosis at the basolateral surface . ^^^ Moreover , specific stimulation of p75NTR by NGF activated TrkA and the MAPK ( mitogen activated protein kinase ) pathway . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The highs and lows of an NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In ALS , motor neurons re express the p 75 neurotrophin receptor ( p75NTR ) and might become vulnerable to NGF . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
It activated NGF receptor , TrkA and its downstream signaling pathways in PC 12 and promoted the survival of neuronally differentiated PC 12 cells deprived of serum . ^^^ The polypeptide could also bind plasmid DNA and enhance polycation mediated gene delivery in NGF receptor expressing PC 12 cells , but not in COS 7 cells lacking NGF receptors . ^^^ The enhancement of gene transfer in PC 12 was inhibited by pretreatment of free , unbound polypeptides , suggesting a NGF receptor specific effect of the polypeptide . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To discriminate these effects we used two p75NTR receptor positive lines of cholinergic neuroblastoma cells , SN 56 and T 17 that are devoid of or express high affinity NGF ( TrkA ) receptors , respectively . cAMP and retinoic acid caused differentiation of both cell lines . ^^^ These effects of NGF were prevented by an antibody against the p75NTR receptor . ^^^ Data indicate that : ( 1 ) positive cholinotrophic effects of NGF required activation of both TrkA and p75NTR receptors ; ( 2 ) cAMP / RA evoked differentiation inhibited NGF effects mediated by TrkA receptors and activated its p75NTR dependent suppressing influences and ( 3 ) a differentiation evoked decrease of mitochondrial acetyl CoA and an elevation of mitochondrial Ca could augment impairment of cholinergic neurons by neurotoxic signals . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Colonic NGF and NGF receptor expression were determined at 14 days and 12 weeks of age . ^^^ Overexpression of NGF receptor messenger RNA , present at 14 days , was not observed later . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NGF controls dendrite development in hippocampal neurons by binding to p75NTR and modulating the cellular targets of Notch . ^^^ The NGF effects on both Hes1 / 5 expression and dendrite morphology were prevented by p 75 antibody ( a p75NTR blocking antibody ) or transfection with enhancer of split 6 ( Hes 6 ) , a condition known to suppress Hes activity . ^^^ The convergence of the Notch and p75NTR signaling pathways at the level of Hes1 / 5 illuminates an unexpected mechanism through which a diffusible factor ( NGF ) could regulate dendrite growth when cell cell interaction via Notch is not in action . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Because SCN neurons do not express TrkA , NGF might have exerted its effects either through direct signalling of the neurons via p75NTR or by enhancing the activity of the cholinergic afferents to the SCN , which arise from the nucleus basalis magnocellularis ( NBM ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We investigated expression of NGF and its receptors , TrkA and p75NTR , in hepatocellular carcinomas ( HCCs ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In the cell culture model of nerve growth factor ( NGF ) deprived sympathetic neurons , we find that CEP 11004 induced a approximately 3 fold increase in the mRNA and protein levels of TrkA , the NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The peptide activated signal transduction pathways of the NGF receptor TrkA in PC 12 cells and supported the survival of the cells after serum deprivation . ^^^ The peptide mediated gene expression was not affected in PC 12 cells by co incubation with a blocking antibody against the low affinity NGF receptor p 75 and was significantly enhanced in NIH3T3 cells stably transfected with TrkA cDNA , suggesting the involvement of the high affinity NGF receptor TrkA without the participation of p 75 . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Thus , alternate ligand independent pathways of p 75 ( NTR ) activation or additional NGF receptor targets may contribute to motor neuron degeneration in ALS mice . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Both the survival and proliferation of breast cancer cells are strongly stimulated by NGF , mediated by TrkA and p75NTR respectively , utilising signaling pathways similar to those described for neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Unlike NGF , the neuraminidase does not react with the apoptosis causing pan neurotrophin receptor p75NTR . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Dose and age dependent axonal responses of embryonic trigeminal neurons to localized NGF via p75NTR receptor . ^^^ We further show that axonal responses to NGF are absent in TG derived from mice that lack the p 75 neurotrophin receptor ( p75NTR ) . ^^^ Collectively , our results suggest that localized sources of NGF can direct axon outgrowth from trigeminal ganglion in a dose and age dependent fashion , mediated by p75NTR signaling through TrkA expressing axons . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
A recent study showed that the precursor for nerve growth factor ( NGF ) can bind to p75NTR with a high affinity and induces apoptosis of neurons in vitro . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We demonstrate that NGF depolarizes the membrane of PC 12 cells within a minute and by using transfected NIH 3T3 Trk and p 75 cells we show that both the high affinity NGF receptor p 140 ( trk ) and the low affinity NGF receptor or p 75 ( NGF ) may be involved in the depolarization . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The data obtained demonstrated that NGF and its antibody affect bromodeoxyuridine ( BrdU ) incorporation and NGF receptor expression by SVZ progenitor cells in the brain of EAE rats . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We report the identification and characterization of two distinct OEC populations in situ and in primary cell suspensions that differed in number , p 75 NGF receptor expression , and O 4 immunoreactivity . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Sustained MAPK activation is dependent on continual NGF receptor regeneration . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
TrkA is essential components of the high affinity NGF receptor necessary to mediate biological effects of the neurotrophins NGF . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Treatment of PC 12 cells with buthionine sulfoximine ( BSO ) reduced the levels of GSH , a major cellular reductant , and enhanced NGF induced neuronal differentiation , activation of AP 1 and the NGF receptor tyrosine kinase , TrkA . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Because NGF signals through the tyrosine kinase ( TrkA ) NGF receptor and / or the p75NTR receptor , we used rescue experiments with a PC 12 cell mutant lacking TrkA to show that NGF induced PPARgamma activation is dependent on TrkA activation . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
No changes were observed in the levels of the NGF peptide or low affinity p 75 neurotrophin receptors ( p75NTR ) , however . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Moreover , C 3 increases axonal outgrowth in the rat hippocampus , raising the possibility of a NGF receptor mediated neuroprotection . ^^^ In order to establish the effect of C 3 on the expression of the NGF receptor IR elements , C 3 was administered orally ( 25 mg / kg , once a day ) , by gavage , 7 days before , and 7 days after the AF64A injection . ^^^ NGF receptor immunohistochemistry revealed that AF64A induced the appearance of NGF receptor IR axonal varicosities in the rat medial septum . ^^^ The increased level of NGF could suppress the increased , AF64A induced NGF receptor expression in the medial septal nucleus . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Differential endocytic sorting of p75NTR and TrkA in response to NGF : a role for late endosomes in TrkA trafficking . ^^^ NGF binds to two receptors , p75NTR and TrkA . ^^^ Both p75NTR and TrkA were internalized in response to NGF and colocalized with early endosomes . ^^^ However , surprisingly , the subsequent endosomal trafficking paths of both NGF receptors diverged : whereas p75NTR recycled back to the surface , TrkA moved to late endosomes and underwent lysosomal degradation . ^^^ Disrupting p75NTR recycling diminished TrkA activation in response to low concentrations of NGF , demonstrating a functional role for the recycling of p75NTR . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
These results suggested that actin driven trafficking of the NGF receptor complex is an essential step for the accumulation and endocytosis of NGF at the growth cone and for the retrograde transport of NGF toward the cell body . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
When normal mybolasts were examined in vivo for expression of NGF receptors , p 75 ( NTR ) was detected on myoblasts but the high affinity NGF receptor , trk A , was not co expressed with p 75 ( NTR ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Furthermore , hyperoxic exposure increased the expression of TrkB in peribronchial smooth muscle but had no effect on the distribution of the specific NGF receptor TrkA . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In embryonic day ( E ) 15 rat trigeminal ganglion ( TG ) , NGF receptor TrkA is expressed by small diameter neurons , NT 3 receptor TrkC and BDNF receptor TrkB are expressed by large diameter neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Surprisingly , the number of choline acetyltransferase containing neurons remains stable despite a significant reduction in NGF receptor positive cells in people with mild cognitive impairment ( MCI ) , suggesting a phenotypic NGF receptor downregulation but not a frank loss of NB neurons during prodromal AD . ^^^ Dysfunction of nerve growth factor ( NGF ) and its high ( TrkA ) and low ( p75NTR ) affinity receptors has been suggested to underlie the selective degeneration of the nucleus basalis ( NB ) cholinergic cortical projection neurons in end stage Alzheimer disease ( AD ) . ^^^ Moreover , there is a loss of cortical TrkA in the face of stable p75NTR and increased proNGF levels , the precursor molecule of mature NGF , in early AD . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To test the hypothesis that trkA ( the high affinity NGF receptor ) is selectively expressed in nociceptive dorsal root ganglion ( DRG ) neurons , we examined the intensity of trkA immunoreactivity in single dye injected rat DRG neurons , the sensory receptor properties of which were identified in vivo with mechanical and thermal stimuli . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
BACKGROUND : Nerve growth factor ( NGF ) and nerve growth factor receptor ( NGFR ) expressions have been found to be increased in sub conjunctival scarring . ^^^ METHODS : Expression of NGF , trkA ( NGFR ) and p75NTR on human fibroblasts grown from conjunctival biopsies and incubated for 2 or 6 days with NGF were evaluated by immunofluorescence , RT PCR , flow cytometry and ELISA . ^^^ RESULTS : NGF induced the expression of p75NTR in the fibroblasts that constitutively expressed only trkA ( NGF ) and increased the migration of wounded fibroblasts , but not their proliferation and collagen production . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Thus , we tested the hypothesis that exposure to ethanol would affect NGF synthesis as well as expression of NGF receptor trkA in this cell population . ^^^ The study results demonstrated that the acute exposure of lipopolysaccharide activated human monocyte / macrophage cultures to ethanol led to a sharp decrease in endogenous produced NGF , which is associated with a reduced expression of high affinity NGF receptor on cell membrane , and to a concomitant impairment of tumor necrosis factor alpha production . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
This study examined the growth capacity of nerve growth factor ( NGF ) responsive dorsal root ganglion ( DRG ) central processes using mice of the following genotypes : wildtype , p 75 neurotrophin receptor ( p75NTR ) exon 3 null mutant , NGF transgenic , and NGF transgenic with p75NTR exon 3 null mutation ( NGF / p75 ( / ) ) . ^^^ Interestingly , NGF / p75 ( / ) mice displayed significantly more new axonal growth when compared to NGF transgenic mice expressing full length p75NTR . ^^^ This collateral growth is restricted to deafferentated areas of the dorsal horn , and we therefore propose that this is an example of compensatory sprouting by NGF sensitive axons in the spinal cord , a response that is enhanced in the absence of NGF binding to p75NTR . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Pro inflammatory cytokines upregulate the skin immunoreactivity for NGF , NT 3 , NT 4 and their receptor , p75NTR in vivo : a preliminary report . ^^^ As a first step toward exploring this , we studied in murine back skin ( C57BL / 6 ) whether intradermally injected interleukin 1beta ( IL 1beta ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) altered the cutaneous immunoreactivity patterns of nerve growth factor ( NGF ) , brain derived neurotrophic factor ( BDNF ) , neurotrophin 3 ( NT 3 ) , neurotrophin 4 ( NT 4 ) , Trk A , Trk B , Trk C and p75NTR and their receptors ( TrkA , TrkB , TrkC , p75NTR ) on the protein level in situ . ^^^ Therefore , this pilot study provides the first preliminary indications that proinflammatory cytokines upregulate the cutaneous immunoreactivity of NGF , NT 3 , NT 4 and their receptor p75NTR in vivo . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Furthermore , EA was able to prevent the EV induced up regulation of p75NTR , probably by normalizing the sympathetic ovarian response to NGF action . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
TrkA NGF receptor plays a role in the modulation of p75NTR expression . ^^^ The cellular response to nerve growth factor ( NGF ) is mediated by two structurally unrelated receptors , TrkA and p 75 neurotrophin receptor ( p75NTR ) , which have been shown to interact resulting in reciprocal modulation of function . ^^^ In this study , we have examined the modulation of p75NTR protein expression by specific TrkA autophosphorylation sites in the presence or absence of NGF . ^^^ In addition , the expression of p75NTR was upregulated in the presence of NGF in the parental and Y490F cell lines only . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The p75NTR dependent effect requires NGF ( nerve growth factor ) binding and activation of the second messenger ceramide . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Kindling , an experimental model of epileptogenesis , and activation induced synaptic reorganization are modulated by nerve growth factor ( NGF ) , but whether NGF acts via its high affinity receptor TrkA and / or the common neurotrophin receptor p75NTR is unknown . ^^^ We previously demonstrated , and confirmed in this study , that inhibition of NGF binding to both TrkA and p75NTR inhibited kindling and decreased kindling induced mossy fiber sprouting . ^^^ NGF binding , but not p75NTR . ^^^ NGF or p75NTR . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In addition , following 2 h exposure of the astrocytes to 10 micromol / L HupA , there was a significant up regulation of mRNA for NGF and P 75 low affinity NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Because recent studies suggest that proNGF , the precursor form of NGF , is more active than mature NGF in inducing apoptosis after binding to p75NTR and a coreceptor , sortilin , we asked whether the precursor of BDNF ( proBDNF ) is also a proapoptotic ligand in the nervous system . proBDNF is secreted by cultured neurons , and recombinant proBDNF binds to sortilin . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
This is the first study that demonstrates the augmentation effect between M 4 mAChR and NGF receptor , and the regulatory role of mAChR on tuberin . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Expression levels of the NGF receptor TrkA , and NGF induced increases in mRNAs for the immediate early genes ( IEGs ) c fos and NGF inducible ( NGFI ) genes NGFI A , NGFI B and NGFI C , were identical among subclones . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In this study we investigated the cell localization of the two main receptors for nerve growth factor ( NGF ) , TrkA and p75NTR , in the cow and pig ovary , using immunohistochemistry . ^^^ Taken together , these results suggest a possible direct role of NGF on oocytes expressing TrkA and p75NTR , in addition to the well known roles in other ovary functions . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Using intrathecal antisense against the NGF receptor , receptor tyrosine kinase ( TrkA ) , we found NGF to act at the primary afferent nociceptor directly in the Sprague Dawley rat . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
It has been associated with mutations in the NTRK 1 gene , located in 1q21 22 and encoding a high affinity NGF receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We developed indirect ELISA methods for : choline acetyltransferase ( ChAT ) ; the vesicular acetylcholine transporter ( VAChT ) ; the high affinity choline transporter ( HACT / CHT ) ; TrkA , the high affinity NGF receptor ; the p 75 neurotrophin receptor ( p 75 ( NTR ) ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NF 1 encodes a GAP for the NGF receptor TrkA , and NF 1 mutations promote survival after NGF withdrawal . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Transient exposure of the cells to 200 microM H2O2 triggered a significant reduction of cell viability and decreased the mRNA and protein levels of NGF , neurotrophin receptor P 75 ( P75NTR ) receptor and tyrosine kinase A ( TrkA ) receptor . ^^^ Incubation of cells with 10 microM huperzine A prior to H2O2 exposure significantly elevated their survival and restored the mRNA and protein levels of NGF , P75NTR receptor and TrkA receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
These results suggest the signal transduction pathway from NGF receptor TrkA to transcription factor Sp 1 protein binding to the promoter Sp 1 like motif in NGF induced rat SPHK 1 gene expression . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
METHODS : Western blotting , RT PCR , transcription factor specific reporter gene systems , mutant cells lacking the low affinity p75NTR NGF receptor and transfections of TNFalpha / NGF chimeric receptors were used to investigate signalling events associated with NGF / TNFalpha promoted iNOS induction in PC 12 cells . ^^^ Furthermore , cells transfected with chimeric TNFalpha / NGF receptors demonstrated that the simultaneous presence of both p75NTR and TrkA signaling is necessary to synergize with TNFalpha to mediate iNOS expression . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Rat weight , estrous cyclicity , ovarian morphology , ovarian mRNA , and protein expression of alpha 1 AR subtypes , beta 2 AR , the NGF receptor tyrosine kinase A ( TrkA ) , p 75 neurotrophin receptor ( p75NTR ) , and tyrosine hydroxylase ( TH ) were analyzed . ^^^ The results indicate that the NGF / NGF receptor system plays a role in the pathogenesis of EV induced PCO in rats . . ^^^ Effect of anti NGF on ovarian expression of alpha 1 and beta 2 adrenoceptors , TrkA , p75NTR , and tyrosine hydroxylase in rats with steroid induced polycystic ovaries . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We analyzed the expression of basic fibroblast growth factor ( bFGF ) and nerve growth factor ( NGF ) and their receptor ( bFGFR and NGFR ) like immunoreactivity in chick basilar papillae , along with bFGF and NGF mRNA expression . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Nerve growth factor ( NGF ) contents in sciatic nerves and the expression of NGF receptor TrkA in DRGs were decreased . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In this study , we investigated alterations in the NGF pathway in keratoconus affected corneas and found a total absence of the NGF receptor TrkA ( TrkA ( NGFR ) ) expression and a decreased expression of NGF and p 75 ( NTR ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The effect of exercise on ( 1 ) ovarian morphology ; ( 2 ) mRNA and protein expression of nerve growth factor ( NGF ) ; and ( 3 ) mRNA and number of ovarian expressing cells for the NGF receptor ( p 75 neurotrophin receptor ) and the alpha ( 1a ) , alpha ( 1b ) , alpha ( 1d ) and beta ( 2 ) adrenergic receptors ( ARs ) in rats with EV induced PCO was evaluated . ^^^ The results obtained indicated that ovarian morphology was almost normalised in the PCO exercise group ; NGF mRNA and protein concentrations were normalised in the PCO exercise group ; high numbers of NGF receptor expressing cells in PCO ovaries were lowered by exercise ; and the number of immunopositive cells of the different AR subtypes were all reduced after exercise in the PCO group , except for the alpha ( 1b ) and beta ( 2 ) AR whereas the mRNA levels were unaffected , indicating transcriptional regulation . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
OBJECTIVE : To assess changes associated with diabetes in urinary bladder nerve growth factor ( NGF ) and NGF receptor p75NTR expression using a streptozotocin ( STZ ) induced diabetic rat model . ^^^ Expressions of the mRNAs that encoded NGF and NGF receptor p75NTR in the rat bladder were evaluated using reverse transcription polymerase chain reaction . ^^^ The mRNA expressions of bladder NGF and p75NTR in the diabetic rats were significantly lower than in the control group ( P < 0 . 05 and < 0 . 001 , respectively ) . ^^^ Treatment with either insulin or phlorizin restored the normal blood sugar level ; moreover , the bladder NGF levels and the expressions of both NGF and p75NTR mRNAs were normal . ^^^ CONCLUSION : The decrease in the genetic expression of NGF and p75NTR might be responsible for the pathogenesis of diabetic cystopathy while hyperglycaemia is part of the cause of these changes . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Meanwhile , nerve growth factor ( NGF ) is critically involved in cell survival and neuroprotective effects via the extracellular signal related kinase ( ERK ) pathway or the phosphatidylinositol 3 kinase ( PI 3 K ) pathway mediated by the high affinity NGF receptor , tropomyosin related kinase A ( TrkA ) . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In chick retina , migratory RGCs express p 75 ( NTR ) , whereas layered RGCs express the high affinity NGF receptor TrkA , which may switch the pro apoptotic signaling of p 75 ( NTR ) into a neurotrophic one . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The expression of NGF is high in injured and inflamed tissues , and activation of the NGF receptor tyrosine kinase trkA on nociceptive neurons triggers and potentiates pain signalling by multiple mechanisms . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
In the present study , we have investigated the expression profile of neurotrophins NGF , BDNF and NT 3 and their high affinity ( TrkA , TrkB , TrkC ) and common low affinity ( p75NTR ) receptors before and during neural differentiation of rat BMSCs by RT PCR . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
However , it is unknown whether human hair growth is also controlled through p75NTR , its high affinity ligand pro NGF , and / or the growth promoting high affinity NGF receptor tyrosine kinase A ( TrkA ) . ^^^ Nerve growth factor ( NGF ) and its apoptosis promoting low affinity receptor ( p75NTR ) regulate murine hair cycling . ^^^ In microdissected human scalp anagen hair bulbs , mRNA for NGF , pro NGF , p75NTR , and TrkA was transcribed . ^^^ Immunohistomorphometry and in situ hybridization detected strong NGF and pro NGF expression in terminally differentiating inner root sheath keratinocytes , whereas TrkA was co expressed with p75NTR in basal and suprabasal outer root sheath keratinocytes . ^^^ During spontaneous catagen development of organ cultured human anagen hair follicles , p75NTR mRNA levels rose , and p75NTR and pro NGF immunoreactivity increased dramatically in involuting compartments primarily devoid of TrkA expression . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Blockade of NGF , using either an endogenous antibody or an antibody against the NGF receptor , prevents neural plasticity and bladder overactivity in experimental models of these conditions . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
We show that NGF receptor complexes have two distinct diffusive states , characterized as mobile and immobile phase . ^^^ The abrupt transition to the immobile phase often occurred simultaneously with the clustering of NGF receptor complexes . ^^^ Immobilization depended on the phosphorylation of the TrkA NGF receptor . ^^^ Using dual color imaging , it was demonstrated that the membrane recruitment of the intercellular signaling protein occurs with NGF receptor complexes in the immobile phase indicating signal transduction occurs during this phase . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Under normal conditions , expression of the p 75 neurotrophin receptor ( p75NTR ) by sympathetic neurons can increase the affinity of the signaling receptor , trkA , to target derived nerve growth factor ( NGF ) at distal axons . ^^^ We have previously reported that sprouting of sympathetic axons into NGF rich target tissues is enhanced when p75NTR expression is perturbed , leading to the postulate that p75NTR may restrain sympathetic sprouting in response to elevated NGF levels . ^^^ Since mice carrying a null mutation of the fourth p75NTR exon may not express a similar splice variant , we sought to determine whether these animals possess the same phenotype of enhanced sympathetic sprouting in response to elevated levels of NGF . ^^^ Both lines of transgenic mice lacking p75NTR displayed similar degrees of sympathetic axonal sprouting into the cerebellum and trigeminal ganglia , two target tissues having elevated levels of NGF protein . ^^^ Furthermore , the densities of sympathetic axons in both targets were significantly greater than those observed in age matched NGF transgenic siblings expressing full length p75NTR . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Bex 1 competed with RIP 2 for binding to the p75NTR intracellular domain , and elevating RIP 2 levels restored the ability of cells overexpressing Bex 1 to differentiate in response to NGF . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NGF in turn induces apoptosis in co cultured motor neurons expressing the p 75 neurotrophin receptor ( p75NTR ) by a mechanism involving nitric oxide ( NO ) and peroxynitrite formation . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
However , binding of neurotrophins , including brain derived neurotrophic factor ( BDNF ) and nerve growth factor ( NGF ) , to the p 75 neurotrophin receptor ( p75NTR ) abolished the neuregulin anti apoptotic effect on motor neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Notch and NGF / p75NTR control dendrite morphology and the balance of excitatory / inhibitory synaptic input to hippocampal neurones through Neurogenin 3 . ^^^ We have previously shown that dendrite morphology of cultured hippocampal neurones is controlled by Notch receptor activation or binding of nerve growth factor ( NGF ) to its low affinity receptor p75NTR , i . e . processes that up regulate the expression of the Homologue of enhancer of split 1 and 5 . ^^^ Hence , a deficit of Notch or NGF / p75NTR activation can lead to the production of high levels of Neurogenin 3 , which stimulates the outgrowth of new dendrites . ^^^ Our results reveal a hitherto unknown contribution of NGF / p75NTR to dendritic and synaptic plasticity through Neurogenin 3 signalling . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Furthermore , since mutations to the NGF receptor , TrkA , have been noted to alter the behaviour of PC 12 cells in response to NGF , a PC 12 subline that expresses a mutated TrkA receptor was utilized to illustrate that a Y785F mutation in the cytoplasmic tail of TrkA results in increased migration in response to the stimulus compared to the control PC12s . . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Studies showing that neurotrophin binding to p75NTR can promote cell survival in the absence of Trk ( tropomyosin related kinase ) receptors , together with recent structural data indicating that NGF may bind to p75NTR in a monovalent manner , raise the possibility that small molecule p75NTR ligands that positively regulate survival might be found . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Interestingly in the cornea , where NGF , trkA ( NGFR ) and p 75 ( NTR ) are expressed by epithelial cells and keratocytes , the NGF eye drop induces the healing of neurotrophic or autoimmune corneal ulcers . ^^^ NGF , trkA ( NGFR ) and p 75 ( NTR ) were found expressed by fibroblastic keratocytes . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Indeed , nerve growth factor ( NGF ) seems to promote axon growth through binding to its p75NTR receptor and inactivating RhoA . ^^^ In cultured hippocampal neurons , the capacity of NGF to promote axon elongation is mostly mediated by p75NTR , and the activation of this pathway leads to the inactivation of GSK 3beta . ^^^ However , the signaling pathway triggered by NGF / p75NTR acts through casein kinase 2 ( CK 2 ) . ^^^ NGF / p75NTR activated CK 2 phosphorylates the phosphatase and tensin homologue deleted on chromosome 10 ( PTEN ) , thus rendering this phosphatase inactive . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Binding to isolectin B 4 ( IB 4 ) and expression of tyrosine kinase A ( trkA ) ( the high affinity NGF receptor ) have been used to define two different subgroups of nociceptive small dorsal root ganglion ( DRG ) neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
BDNF , through p75NTR , promotes apoptosis and inhibits axonal growth of sympathetic neurons , antagonizing the pro survival and axon growth promoting actions of NGF through TrkA . ^^^ While the trafficking of the TrkA : NGF complex is well characterized , little is known about p75NTR : BDNF trafficking in these neurons . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NGF receptor TrkAd 5 : therapeutic agent and drug design target . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
To further explore details of this mechanism , the expression of nerve growth factor ( NGF ) , production of which is induced by inflammatory cells and of the NGF receptor TrkA was examined in the PNS and the dorsal root ganglion using immunohistochemistry and a ribonuclease protection assay . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The neurotrophin family with its first member , nerve growth factor ( NGF ) , binds two classes of receptors , more specifically to Trk receptors and to a shared p75NTR receptor . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
The compound induced sustained extracellular signal regulated kinase ( ERK ) activation that is crucial for neurite outgrowth activity without activating NGF receptor , TrkA . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
Pro NGF from Alzheimer ' s disease and normal human brain displays distinctive abilities to induce processing and nuclear translocation of intracellular domain of p75NTR and apoptosis . ^^^ The pro form of neurotrophic growth factor ( pro NGF ) , purified by chromatography from human Alzheimer ' s disease ( AD ) affected brains ( ADhbi pro NGF ) , has been shown to induce apoptotic cell death in neuronal cell cultures through its interaction with the p 75 neurotrophin receptor ( p75NTR ) . ^^^ In the present work , we report that ADhbi pro NGF stimulates processing of p75NTR with alpha and gamma secretases , yielding a 20 kd intracellular domain ( p 75 ( ICD ) ) that translocates to the nucleus . ^^^ Although human frontal cortex has been described as showing a higher pro NGF increase in AD , the increase in the entorhinal cortex paralleled p75NTR processing in its intracellular domain . ^^^
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01138 and P08138	Pubmed	SVM Score :0.0
NA