| Interacting proteins: P01138 and P01023 |
Pubmed |
SVM Score :0.54739261 |
| The objectives of this study are to determine whether alpha 2M can also be modified by larger aromatic biogenic amines such as 5 hydroxytryptamine ( 5HT ; serotonin ) , the nature of interaction between NGF and 5HT modified alpha 2 M ( 5HT alpha 2M ) , and the effect of 5HT alpha 2M on the neurite extension and the growth of embryonic sensory and cholinergic neurons in 2 disparate animal species ( chicken and rats ) . 0.54739261^^^ As determined by gel filtration and polyacrylamide gel electrophoresis studies , both 5HT alpha 2M and normal alpha 2M combine noncovalently with NGF , but 5HT alpha 2M by comparison can combine with NGF somewhat more effectively . 0.54629068^^^ |
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| Interacting proteins: P01138 and P01023 |
Pubmed |
SVM Score :0.0 |
| Monoamine activated alpha 2 macroglobulin ( alpha 2M ) has been shown to inhibit beta nerve growth factor ( NGF ) promoted neurite outgrowth and the survival of embryonic sensory and forebrain neurons , whereas normal alpha 2M has little or no such activity . ^^^ |
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| Interacting proteins: P01138 and P01023 |
Pubmed |
SVM Score :0.0 |
| In serum , nerve growth factor ( NGF ) forms a complex with alpha 2 macroglobulin ( alpha 2M ) , which formation inhibits the immunoreactivity between NGF and its antibodies . ^^^ For measuring the serum level of NGF , it is thus necessary to liberate NGF from the NGF alpha 2M complex and prevent reformation of such complex . ^^^ |
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| Interacting proteins: P01138 and P01023 |
Pubmed |
SVM Score :0.0 |
| The objective of this study is to compare the NGF binding properties of methylamine modified human alpha 2M ( MA alpha 2M ) versus normal alpha 2M and their effects on the biological activity of NGF and neurite extension by embryonic chicken dorsal root ganglia . ^^^ As determined by gel filtration , polyacrylamide gel electrophoresis , and equilibrium binding studies , these two forms of alpha 2M are similar in their binding affinities , with MA alpha 2M binding about twice as much NGF as normal alpha 2M . ^^^ Both normal alpha 2M and MA alpha 2M combine noncovalently with NGF , and prior modification of alpha 2M is unnecessary for the binding to occur . ^^^ In contrast to normal alpha 2M , MA alpha 2M potently inhibits the biological activity of NGF and exerts a dose dependent inhibition on the NGF stimulated neurite outgrowth by embryonic chicken dorsal root ganglia in culture . ^^^ The inhibitory effect of MA alpha 2M can be overcome by higher NGF concentrations , but is irreversible at lower NGF concentrations . ^^^ |
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| Interacting proteins: P01138 and P01023 |
Pubmed |
SVM Score :0.0 |
| Recent studies implicate alpha 2 macroglobulin ( alpha 2M ) as a protein that regulates the distribution and activity of many cytokines , including transforming growth factors beta ( TGFs beta ) , tumor necrosis factor alpha ( TNF alpha ) , platelet derived growth factor ( PDGF ) , interleukin 6 ( IL 6 ) , nerve growth factor ( NGF ) , fibroblast growth factor ( b FGF ) , and interleukin 1 beta ( IL 1 beta ) . ^^^ Some cytokines , including PDGF , NGF , and IL 6 bind preferentially to the native secreted form of alpha 2M , whereas the TGF beta s , TNF alpha and IL 1 beta bind preferentially to forms of alpha 2M that have been modified by proteinases such as plasmin . ^^^ |
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| Interacting proteins: P01138 and P01023 |
Pubmed |
SVM Score :0.0 |
| On polyacrylamide isoelectric focussing gels mouse and human S NGF could be recovered from the same position as NGF as well as from the region where alpha 2 macroglobulin and serum albumin focused . ^^^ |
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| Interacting proteins: P01138 and P01023 |
Pubmed |
SVM Score :0.0 |
| As little as 0 . 35 microM monoamine activated alpha 2M can suppress the ChAT activity , whereas either nerve growth factor ( NGF ) or brain derived neurotrophic factor ( BDNF ) , but not neurotrophin 3 ( NT 3 ) , stimulates ChAT expression of these cells . ^^^ The addition of either NGF or BDNF to the alpha 2M suppressed cells can increase ChAT activity back to its normal levels , while NT 3 can not . ^^^ These results demonstrate that ( 1 ) monoamine activated alpha 2M is a potent non cytotoxic inhibitor of the ChAT activity in cholinergic basal forebrain neurons , and ( 2 ) NGF and BDNF are capable of not only stimulating the ChAT activity but can also specifically reverse the alpha 2M inhibition . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^ Monoamine activated alpha 2 macroglobulin inhibits choline acetyltransferase of embryonic basal forebrain neurons and reversal of the inhibition by NGF and BDNF but not NT 3 . ^^^ |
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| Interacting proteins: P01138 and P01023 |
Pubmed |
SVM Score :0.0 |
| The kallikrein like serine proteinase nerve growth factor gamma ( NGF gamma ) reacted with the plasma proteinase inhibitor human alpha 2 macroglobulin ( h alpha 2M ) . ^^^ In N alpha benzoyl DL arginine p nitroanilide ( BAPNA ) hydrolysis experiments , the catalytic efficiency ( kcat / KM ) of the h alpha 2M NGF gamma complex was decreased by 98 . 5 % compared with free NGF gamma . ^^^ The rate of NGF gamma inhibition by h alpha 2M was ( 1 . 0 + / 0 . 1 ) 10 10 ( 4 ) M 1 s 1 as determined by BAPNA hydrolysis . ^^^ NGF gamma , which was bound within the intact 7S NGF complex , also reacted with h alpha 2M , albeit at a very slow rate . ^^^ NGF gamma was rapidly inhibited by murine alpha 2M ( m alpha 2M ) . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^ |
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| Interacting proteins: P01138 and P01023 |
Pubmed |
SVM Score :0.0 |
| We conclude that NGF and NT 3 appear to bind specifically in significant quantities to the same alpha 2M sites ; but the other cytokines by comparison bind minimally , and primarily or entirely use nonspecific molecular interactions in their binding to alpha 2M . . ^^^ Comparative binding of neurotrophins ( NT 3 , CNTF and NGF ) and various cytokines to alpha 2 macroglobulin . ^^^ |
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| Interacting proteins: P01138 and P01023 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P01138 and P01023 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P01138 and P01023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01138 and P01023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01138 and P01023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01138 and P01023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01138 and P01023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01138 and P01023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01138 and P01023 |
Pubmed |
SVM Score :0.0 |
| NA |
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