| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.59022929 |
| To further dissect the ligand binding selectivity of the ErbB network , we have applied the phage display technique to examine the role of the linear N terminal region in EGF for interaction with ErbB 2 / ErbB 3 heterodimers . 0.59022929^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| The EGF receptor family was recently expanded with the sequencing of HER 3 and HER 4 . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| The c erbB 3 gene is a member of the EGF receptor family of membrane tyrosine kinases . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| TPA did not induce the degradation of related receptors ( ErbB 1 , ErbB 2 , and ErbB 3 ) in the EGF receptor family . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| HB EGF binds to NIH 3T3 cells overexpressing either HER 1 or HER 4 alone , but not HER 2 or HER 3 alone . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| EGF signals through EGFR , whereas heregulin ( HRG ) signaling is initiated through binding to either ErbB 3 or ErbB 4 . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| These include EGF receptor ( erbB 1 ) and erbB 4 when expressed individually and erbB 2 and erbB 3 when expressed together . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| The erbB 3 gene encodes a transmembrane protein that is related to the epidermal growth factor ( EGF ) receptor and erbB 2 . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Epidermal growth factor ( EGF ) receptor tyrosine kinases include ErbB 1 , ErbB 2 , ErbB 3 and ErbB 4 . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| In this report we show that ErbB 2 , ErbB 3 , and ErbB 4 do not potentiate Stat 5 phosphorylation by EGF . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| BTC induced phosphorylation of all three EGF receptors present on HUVECs : ErbB 2 , ErbB 3 , and ErbB 4 . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| The presence of the EGF receptors EGFR , ErbB 2 , and ErbB 3 was confirmed through Western blot analysis of cell lysates . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| BTC induced phosphorylation of all four EGF receptors present on HTASMCs : ErbB 1 , ErbB 2 , ErbB 3 , and ErbB 4 . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Two members of the EGF receptor family , HER 2 and HER 3 , act as key oncogenes in breast cancer cells . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| The family currently comprises three closely related members : the epidermal growth factor ( EGF ) receptor , c erbB 2 and c erbB 3 , all of which show abnormalities of expression in various human tumours . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Expression of the c erbB 3 protein in normal human adult and fetal tissues . c erbB 3 is a member of the type 1 family of growth factor receptors which includes the epidermal growth factor ( EGF ) receptor and c erbB 2 . ^^^ Whereas for EGF receptor and c erbB 2 the expression patterns in normal tissues are well documented , there is currently little information available about the sites of c erbB 3 expression . ^^^ The c erbB 3 protein was identified in cells of the gastrointestinal , reproductive , respiratory and urinary tracts as well as the skin , endocrine and nervous system in a distribution distinctly different from that observed for EGF receptor and c erbB 2 . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Human EGFR 3 ( HER 3 ) / ERBB3 is a recently identified protein related to the EGFR that is widely expressed in breast carcinomas and is a candidate receptor for EGF like growth factors . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Employing reduced stringency hybridization with 5 erbB as a probe , we recently identified a third member of this receptor family , designated erbB 3 . cDNA cloning revealed a predicted 148 kD transmembrane polypeptide with structural features similar to those of the EGF receptor . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Here we show that in A 431 cells , which express both the EGF receptor and ErbB 3 , PtdIns 3 kinase coprecipitates with the ErbB 3 protein ( p180erbB3 ) in response to EGF . p180erbB3 is also shown to be tyrosine phosphorylated in response to EGF . ^^^ Thus , we show for the first time that ErbB 3 can mediate EGF responses in cells expressing both ErbB 3 and the EGF receptor . . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Like heregulin , epidermal growth factor ( EGF ) also stimulate the tyrosine phosphorylation of both Neu and ErbB 3 . ^^^ However , there are clear differences between the EGF and heregulin stimulated phosphorylations of ErbB 3 . ^^^ In the EGF response , at least two tyrosine phosphorylated forms of ErbB 3 are detected , but these phosphoproteins have distinctly lower apparent molecular weights compared with the heregulin stimulated ErbB 3 phosphoproteins and do not complex with p 85 . ^^^ Thus the formation of a stable ErbB 3 p85 complex in PC 12 cells is a unique outcome of heregulin signaling that correlates with the differences in cell morphology induced by the activated EGF receptor and the Neu tyrosine kinase . . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| We have shown previously that binding of Shc to the epidermal growth factor ( EGF ) like receptor , c ErbB 3 , is through an NPXY motif ( Prigent , S . ^^^ In this paper we have used fusion proteins of various Shc domains to show that it is the N terminal domain of Shc that is primarily responsible for binding EGF receptor and c ErbB 3 . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Heregulin is a ligand for the erbB 3 and erbB 4 receptors , with a region of high homology to epidermal growth factor ( EGF ) . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Since NDF , HER 2 / neu , and HER 3 are present in skin , and other EGF family members can influence wound keratinocytes in vivo , we investigated whether NDF would stimulate epidermal migration and proliferation in a rabbit ear model of excisional wound repair . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Identification of c erbB 3 binding sites for phosphatidylinositol 3 ' kinase and SHC using an EGF receptor / c erbB 3 chimera . c erbB 3 is a member of the type 1 ( EGF receptor related ) family of growth factor receptors for which no ligand has been identified . ^^^ In this study we have shown that SHC and phosphatidylinositol 3 ' kinase bind to the activated EGF receptor / c erbB 3 chimera . ^^^ In contrast to EGF receptor and c erbB 2 , we were unable to detect binding of activated c erbB 3 to GRB 2 . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| ErbB 3 is a member of the epidermal growth factor ( EGF ) receptor subfamily of receptor tyrosine kinases and is believed to be a receptor for an unknown ligand . ^^^ We have tested the possibility that heregulin , a growth factor possessing an EGF like domain , is a ligand for ErbB 3 . ^^^ We have found that the iodinated recombinant EGF like domain of heregulin beta 1 ( 125I rHRG beta 1 ( 177 244 ) bound specifically to insect cell expressed bovine ErbB 3 with a dissociation constant of 0 . 85 nM . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| An EGFR / ErbB 3 chimera was expressed on the surface of NIH 3T3 transfectants as two classes of receptors possessing epidermal growth factor ( EGF ) binding affinities comparable to those of the wild type EGF receptor ( EGFR ) . ^^^ EGF induced autophosphorylation in vivo of the chimeric receptor and DNA synthesis of EGFR / ErbB 3 transfectants with a dose response similar to that of EGFR transfectants . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Previously we demonstrated that EGF dependent activation of PI 3 kinase activity in A 431 cells is accompanied by the binding of p 85 to ErbB 3 , an EGF receptor homologue . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Furthermore , neu differentiation factor / heregulin , a ligand that indirectly activates p185erbB 2 by direct binding to erbB 3 receptors , potently stimulated the proliferation of the growth factor dependent H16N 2 cells ( which expressed c erbB 2 and c erbB 3 but not c erbB 4 ) in the absence of both IGF and EGF . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| We show that in various cells ErbB 2 can form heterodimers with both EGF receptor ( ErbB 1 ) and NDF receptors ( ErbB 3 and ErbB 4 ) , suggesting that it may affect the action of heterologous ligands without the involvement of a direct ErbB 2 ligand . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Four transmembrane tyrosine kinases constitute the ErbB receptor family : the epidermal growth factor ( EGF ) receptor , ErbB 2 , ErbB 3 , and ErbB 4 . ^^^ We have measured the endocytic capacities of all four members of the EGF receptor family , including ErbB 3 and ErbB 4 , which have not been described previously . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| While several EGF agonists bind and activate ErbB 1 / EGF receptor , neu differentiation factor ( NDF ) functions as a ligand for ErbB 3 and ErbB 4 . ^^^ The ability to induce tyrosine phosphorylation of ErbB 3 varied dramatically among the different EGF related peptides . ^^^ While EGF , transforming growth factor ( TGF ) alpha , and amphiregulin only had a moderate effect , NDF dramatically increased the ErbB 3 phosphotyrosine content . ^^^ Consequently , only NDF , HB EGF , and BTC significantly stimulated association of phosphatidylinositol kinase activity with ErbB 3 . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| The group of subtype 1 transmembrane tyrosine kinases includes the epidermal growth factor ( EGF ) receptor ( ErbB 1 ) , an orphan receptor ( ErbB 2 ) , and two receptors for the Neu differentiation factor ( NDF / heregulin ) , namely : ErbB 3 and ErbB 4 . ^^^ By using the generated mAbs , we found that the major NDF receptor on mammary epithelial cells is a heterodimer of ErbB 3 with ErbB 2 , whereas an ErbB 1 / ErbB 2 heterodimer , or an ErbB 1 homodimer , is the predominant species that binds EGF . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| The ErbB family of transmembrane tyrosine kinases includes the receptor for EGF ( ErbB 1 ) , two receptors for NDF / heregulin ( ErbB 3 and ErbB 4 ) and an orphan receptor ( ErbB 2 ) . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| In addition , the neu differentiation factor / heregulins ( HRGs ) , a family of ligands that activate p185erbB 2 through direct binding to erbB 3 or erbB 4 , are potent mitogens for various nonneoplastic mammary epithelial cells and carcinoma cell lines in the absence of both IGF and EGF in culture . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Whereas ErbB 1 binds to the epidermal growth factor ( EGF ) , both ErbB 3 and ErbB 4 bind to the Neu differentiation factors ( NDFs , or neuregulins ) , and ErbB 2 , the most oncogenic family member , is an orphan receptor whose function is still unknown . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Overexpression of growth factor receptors ( EGF receptor , c erbB 2 , c erbB 3 , TGF beta receptor 1 3 ) , growth factors ( EGF , TGF alpha , TGF beta 1 3 , aFGF , bFGF ) , adhesion molecules ( ICAM 1 , ELAM 1 ) and gene mutations ( p 53 , K ras , DCC , APC ) are present in a significant number of these tumors . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Stimulation of NRG receptors , erbB 3 and erbB 4 initiates oligomerization between these receptors or between these receptors and other members of the epidermal growth factor ( EGF ) receptor family , resulting in stimulation of their associated tyrosine kinase activities . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Expression of the type 1 tyrosine kinase growth factor receptors EGF receptor , c erbB 2 and c erbB 3 in bladder cancer . ^^^ Overexpression of the epidermal growth factor ( EGF ) receptor ( 36 per cent , 25 / 70 ) and c erbB 2 proteins ( 9 per cent 6 / 70 ) was determined in the same series of cases . c erbB 3 overexpression was positively correlated with EGF receptor expression ( P < 0 . 025 ) but appeared to be inversely associated with c erbB 2 overexpression . . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| EGF R , ErbB 3 , and ErbB 4 were not detected in the CHK immunoprecipitates or in the precipitates of the GST SH 2 fusion proteins of CHK , suggesting that the association of CHK with ErbB 2 upon heregulin stimulation is receptor specific ( ErbB 2 ) and ligand specific ( heregulin ) . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| The EGF family includes EGF , transforming growth factor alpha , heparin binding EGF , amphiregulin , beta cellulin , epiregulin , and heregulins , whereas the receptor family ( the erbB genes ) consists of erbB 1 ( EGF receptor , EGF R ) , erbB 2 , erbB 3 , and erbB 4 . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| They also provide an explanation for previously reported dominant negative mutants of the EGF receptor and have interesting implications for the signaling through homo and heterodimers of the family members : EGF receptor , erbB 2 , erbB 3 , and erbB4 . . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Three signal transducing transmembrane receptors for neuregulins , which bear significant homology to the EGF receptor , are currently known : HER 2 ( erbB 2 ) , HER 3 ( erbB 3 ) , and HER 4 ( erbB 4 ) . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Down regulation of cell surface ErbB 1 or ErbB 2 by intracellular expression of specific antibodies has allowed us to delineate the role of these receptors during signaling elicited by : EGF and heparin binding EGF ( HB EGF ) , ligands of ErbB 1 ; betacellulin ( BTC ) , a ligand of ErbB 1 and ErbB 4 ; and neu differentiation factor ( NDF ) , a ligand of ErbB 3 and ErbB 4 . ^^^ Furthermore , EGF and BTC induced activation of ErbB 3 is impaired in the absence of ErbB 2 , suggesting that ErbB 2 has a role in the lateral transmission of signals between other ErbB receptors . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| In contrast to the results with erbB 2 , EGF did not induce tyrosine phosphorylation , the degradation of erbB 3 , or erbB 3 : EGFr heterodimer formation in cultured keratinocytes . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| A fluorescence assay previously used to characterize the nucleotide binding properties of the EGF receptor indicated that the ErbB 3 protein was unable to bind nucleotide . ^^^ The hisTKD B 3 protein was subsequently found to be an excellent substrate for the EGF receptor protein tyrosine kinase , which suggested that in vivo phosphorylation of ErbB 3 in response to EGF could be attributed to a direct cross phosphorylation by the EGF receptor protein tyrosine kinase . . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Neu differentiation factor ( NDF ) , a member of the epidermal growth factor ( EGF ) related peptide family , activates ErbB 2 via heterodimerization with the NDF receptors ErbB 3 and ErbB 4 . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Here , we report that EGF specifically binds to and can be crosslinked to 32D transfectants co expressing ErbB 2 and ErbB 3 ( 32D . ^^^ E2 / E3 ) , but not to transfectants expressing either ErbB 2 or ErbB 3 individually . [ 125I ] EGF crosslinked species detected in 32D . ^^^ Additional data utilizing MDA MB 134 human breast carcinoma cells , which naturally express ErbB 2 and ErbB 3 in the absence of EGFRs , supported the results obtained employing 32D cells and suggest that EGF and BTC may contribute to the progression of carcinomas that co express ErbB 2 and ErbB3 . . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| The expression of genes involved in mitotic signal pathway ( EGF , TGF alpha , EGF R , erbB 2 , erbB 3 , c myc and c H ras ) was determined immunocytochemically . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Here we discuss several characteristics of hormone receptor interactions and receptor coupling that contribute to specificity : 1 ) a single EGF family hormone can bind multiple receptors ; 2 ) a single ErbB family receptor can bind multiple hormones ; 3 ) there are three distinct functional groups of EGF family hormones ; 4 ) EGF family hormones can activate receptors in trans , and this heterodimerization diversifies biological responses ; 5 ) ErbB 3 requires a receptor partner for signaling ; and 6 ) ErbB family receptors differentially couple to signaling pathways and biological responses . . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Similarly , EGF treatment of A 431 cells induces concomitant hetero oligomerization of active ErbB 2 with inactive EGFR , of active EGFR with inactive ErbB 2 , and of inactive ErbB 2 with kinase defective ErbB 3 . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Exogenous HB EGF and EGF each activated HER 1 and HER 3 receptor tyrosine kinases and induced tyrosine phosphorylation of cellular proteins to a similar extent . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| In RINm5F cells a spectrum of erbB gene expression was detected ( EGF receptor / erbB 1 , erbB 2 / neu , and erbB 3 ) , whereas INS 1 cells showed only expression of EGF receptor . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| When singly expressed , neither ErbB 3 nor ErbB 2 can mediate signaling by EGF . ^^^ In addition , when co expressed , blocking either receptor by using site specific antibodies inhibited EGF and betacellulin activities , indicating strict cooperativity between ErbB 3 and ErbB 2 . ^^^ Through analysis of chimeras between EGF and TGFalpha , we identified the middle portion of EGF ( loop B ) as the site that enables activation of ErbB 2 / ErbB 3 . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| The receptor ( R ) for epidermal growth factor ( EGF ) is expressed at high levels on human breast cancer cells and associates with ErbB 2 , ErbB 3 , and Src proto oncogene family protein tyrosine kinases ( PTKs ) to form membrane associated PTK complexes with pivotal signaling functions . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Binding interaction of the heregulinbeta egf domain with ErbB 3 and ErbB 4 receptors assessed by alanine scanning mutagenesis . ^^^ Wild type HRGbeta egf domain displayed on phage was properly folded as evidenced by its ability to bind ErbB 3 and ErbB 4 receptor IgG fusion proteins with affinities close to those measured for bacterially produced HRGbeta egf domain . ^^^ Binding to ErbB 3 and ErbB 4 receptors was affected by mutation of residues throughout the egf domain ; including the NH 2 terminus ( His 2 and Leu 3 ) , the two beta turns ( Val 15 Gly18 and Gly 42 Gln46 ) , and some discontinuous residues ( including Leu 3 , Val 4 , Phe 13 , Val 23 , and Leu 33 ) that form a patch on the major beta sheet and the COOH terminal region ( Tyr 48 and Met 50 Phe53 ) . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| The HRGbeta egf domain was displayed on the surface of M 13 phage to facilitate mutagenic analysis and optimize for binding to a homodimeric ErbB 3 immunoglobulin ( IgG ) fusion . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Concentrating on ErbB 1 and two of its ligands , epidermal growth factor ( EGF ) and transforming growth factor alpha ( TGF alpha ) , and the Neu differentiation factor ( NDF / neuregulin ) and one of its receptors , ErbB 3 , we show that ligand binding variably accelerates endocytosis of the respective ligand receptor complex . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Co expression of ErbB 2 , a developmentally important co receptor whose expression is frequently elevated in human cancers , specifically potentiated EGF signaling to the level achieved by TGFalpha , an effect that was partially mimicked by ErbB 3 . ^^^ Analysis of the mechanism underlying this trans potentiation implied that EGF driven homodimers of ErbB 1 are destined for intracellular degradation , whereas the corresponding heterodimers with ErbB 2 or with ErbB 3 , dissociate in the early endosome . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Transcripts for ErbB 3 ( a member of the EGF R subfamily ) , and bFGF R were present in all stages of oocyte maturation , after fertilization up to the 2 cell stage , and the blastocyst stage . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| In order to identify whether EGF like growth factors and corresponding receptors ( erbB 2 , erbB 3 , and erbB 4 ) could be involved in the autonomous growth of these transformed rat thyroid epithelial cells , Northern blot for mRNA analysis and Western blot for protein expression were performed . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| ErbB 3 was phosphorylated in vivo in response to either heregulin ( HRG ) in cells expressing both ErbB 3 and ErbB 2 , or epidermal growth factor ( EGF ) in cells expressing both ErbB 3 and EGF receptor . ^^^ A recombinant receptor protein ( ErbB 3 K / M , in which K / M stands for Lys > Met amino acid substitution ) containing an inactivating mutation in the putative ATP binding site was also phosphorylated in response to HRG and EGF . ^^^ Separate kinase inactivating mutations in the EGF receptor and ErbB 2 proteins abolished ErbB 3 phosphorylation and signal transduction activated by EGF and HRG respectively . ^^^ Hence the protein tyrosine kinase activity necessary for growth factor signalling via the ErbB 3 protein seems to be provided by coexpressed EGF and ErbB 2 receptor proteins . . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Expression of mRNA for heregulin ( HRG ) , a member of the epidermal growth factor ( EGF ) family and its receptors , ErbB 3 and ErbB 4 , were evaluated in human upper gastrointestinal ( GI ) mucosa . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| We show here that following treatment with the progestin , R 5020 , breast cancer cells undergo a `` biochemical shift ' ' in the regulation of epidermal growth factor ( EGF ) stimulated signaling pathways : R 5020 potentiates the effects of EGF by up regulating EGFR , c ErbB 2 and c ErbB 3 receptors , and by enhancing EGF stimulated tyrosine phosphorylation of signaling molecules known to associate with activated type 1 receptors . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| The human epidermal growth factor receptor ( HER or ErbB ) family consists of four distinct members , including the epidermal growth factor ( EGF ) receptor ( EGFR , HER 1 , or ErbB 1 ) , ErbB 2 ( HER 2 or neu ) , ErbB 3 ( HER 3 ) , and ErbB 4 ( HER 4 ) . ^^^ High levels of expression of ErbB 3 were correlated with responsiveness to heregulin and sensory and motor neuron derived factor , whereas high levels of EGFR expression were correlated with responsiveness to EGF and betacellulin . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Ligand induced down regulation of two growth factor receptors , EGF receptor ( ErbB 1 ) and ErbB 3 , correlates with differential ability to recruit c Cbl , whose invertebrate orthologs are negative regulators of ErbB . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| IGF 1 induced PI 3 kinase activation is generally mediated via insulin receptor substrate ( IRS ) 1 , but EGF induced PI 3 kinase activation is mediated by various signalling molecules such as ErbB 3 and c Cbl in different cells . ^^^ Anti SHP 2 and anti p 85 subunits of PI 3 kinase antibodies did not coprecipitate tyrosine phosphorylated EGF receptor or ErbB 3 ; in addition , p 115 and p 105 appeared to be distinct from tyrosine phosphorylated c Cbl . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| By immunoprecipitation followed by Western blotting we showed that ErbB1 / ErbB3 heterodimers are the major mitogenic signaling entity in 1 , 25 ( OH ) 2D3 stimulated cells . 1 , 25 ( OH ) 2D3 did not affect the levels of the proteoglycan dependent EGFR ligands amphiregulin and heparin binding EGF nor the synthesis of proteoglycans , as assessed by 35S labeling and ion exchange chromatography . 1 , 25 ( OH ) 2D3 caused a marked increase in the cellular contents of ErbB 1 , ErbB 2 , and ErbB 3 proteins . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| The epidermal growth factor ( EGF ) receptor ( EGFR ) family includes four homologous transmembrane receptor protein tyrosine kinases , EGFR , ErbB 2 , ErbB 3 , and ErbB 4 . ^^^ ErbB 3 and ErbB 4 are present in HASM cells ; however , EGF has no effect on their activation . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| In contrast , ErbB 3 showed much more restrictive ligand binding specificity and measurable binding was observed only with heregulin , neuregulin2beta , epiregulin and the synthetic heregulin / egf chimera , biregulin . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| The panel of potential SEBs of malignancy include p 53 , cytomorphometric indices , ploidy , PNCA , erbB 2 , erbB 3 , EGF receptor , TGF alpha tumor associated glycoprotein 72 , fatty acid synthetase and Lewis Y antigen . ^^^ A similar pattern of increased biomarker expression between uninvolved and PIN or prostate cancer tissues can be observed for EGF receptor ( mean = 1 . 21 , 1 . 87 and 1 . 76 for uninvolved , PIN and prostate cancer , respectively ) and erbB 3 ( mean = 0 . 81 , 1 . 59 and 1 . 30 for uninvolved , PIN and prostate cancer , respectively ) . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Whereas ErbB 1 binds seven different ligands whose prototype is EGF , the three families of neuregulins ( NRGs ) activate ErbB 3 and / or ErbB 4 . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| To understand the underlying mechanism we constructed a chimeric EGF receptor ( ErbB 1 ) whose autophosphorylation C terminal domain was replaced by the corresponding portion of ErbB 3 . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Activation of ErbB 3 and PI3K was also seen with betacellulin ( BTC ) but not epidermal growth factor ( EGF ) and correlated with the growth effects of these ligands . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| In addition , EGF receptor , c erbB 2 and c erbB 3 levels were reduced , and the basal and EGF stimulated mitogen activated protein kinase activities were reduced . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| The ErbB 2 tyrosine kinase functions as coreceptor for the neuregulin receptors ErbB 3 and ErbB 4 and can participate in signaling of EGF receptor ( ErbB 1 ) , interleukin receptor gp 130 , and G protein coupled receptors . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| We analyzed the formation of homo and heterodimers between EGFR , ErbB 2 , and ErbB 3 ( members of the EGF receptor family ) in the human skin keratinocyte cell line HaCaT , in dependence of the added ligand . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| The increased erbB 3 Tyr phosphorylation is to a significant extent due to transactivation by EGFR as > 70 % of radiation and EGF induced erbB 3 Tyr phosphorylation is inhibited by AG 1478 . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Further studies revealed that , while both EGF ( 100 ng / ml ) and CCh ( 100 microM ) stimulated phosphorylation of the EGFR , only EGF stimulated phosphorylation of ErbB 2 , and neither stimulated ErbB 3 phosphorylation . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| The primary cell surface targets of heregulins are heterodimers of the EGF receptor homolog HER 2 with either HER 3 or HER 4 . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Ligand dependent activation of HER 1 , HER 3 , and HER 4 by EGF or heregulin results in heterodimerization and , thereby , HER 2 activation . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Expression of the EGF receptor family members ErbB 2 , ErbB 3 , and ErbB 4 in germinal zones of the developing brain and in neurosphere cultures containing CNS stem cells . ^^^ The epidermal growth factor receptor family consists of four related tyrosine kinases : the epidermal growth factor receptor ( EGF R or ErbB ) , ErbB 2 , ErbB 3 , and ErbB 4 . ^^^ These receptors are capable of extensive cross activation upon the binding of their ligands the EGF family of peptides for EGF R and the neuregulins for ErbB 3 and ErbB 4 . ^^^ Since EGF R is expressed by proliferating cells in the central nervous system ( CNS ) , including multipotent CNS stem cells , we examined the expression of ErbB 2 , ErbB 3 and ErbB 4 in the germinal epithelia of the developing rat brain using in situ hybridization . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Consistently the predominant interaction after EGF treatment was between EGFR and HER 2 , whereas activation of HER 3 and HER 4 depended on the relative abundance of the four receptors in the cells . ^^^ EGF and HRG mediated cell growth through the EGFR / HER2 heterodimer and HER 4 , respectively , but not through HER 3 when it was the only HRG receptor expressed and phosphorylated in the cells . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| The ErbB receptor tyrosine kinase family consists of the epidermal growth factor ( EGF ) receptor ( ErbB 1 ) and three related receptors ( ErbB 2 , ErbB 3 , ErbB 4 ) . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| The nontransformed H16N 2 cells isolated from normal tissue express EGFR , p 185 ( erbB 2 ) , and erbB 3 , and are highly responsive to the mitogenic effects of HRG beta as well as to the combination of EGF and insulin in serum free culture . ^^^ Furthermore , erbB 3 principally mediated the direct recruitment of p 85 in cells stimulated by HRG or EGF , indicating that , in addition to the high level activation of PI3K by p 185 ( erbB 2 ) / erbB 3 , EGFR / erbB 3 heterodimer interaction is essential for the weak but significant level of PI3K activated by EGF in cells that express normal EGFR levels . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| The HER 3 ECD , purified from Drosophila S 2 cells , binds the EGF like domain of heregulin ( hrg ) with a K ( d ) of 1 . 9 nM as measured by surface plasmon resonance ( SPR ) studies . ^^^ This self association of the HER 3 ECD was reversed by the addition of hrg but not by EGF . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Enhanced drug resistance in cells coexpressing ErbB 2 with EGF receptor or ErbB 3 . ^^^ To further determine the role of the ErbB receptors in drug resistance , we selected various NIH 3T3 cell lines that specifically expressed EGF receptor ( EGFR ) , ErbB 2 , ErbB 3 , EGFR / ErbB2 , EGFR / ErbB3 , or ErbB2 / ErbB3 . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Messenger RNA encoding the EGF receptors ( EGFR ) c erbB 2 and c erbB 3 , but not c erbB 4 , was detected in primary cultures of human nasal epithelial cells . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Expression of the BRK tyrosine kinase in mammary epithelial cells enhances the coupling of EGF signalling to PI 3 kinase and Akt , via erbB 3 phosphorylation . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| The ErbB family of receptors , which includes the epidermal growth factor receptor ( EGFR ) , ErbB 2 , ErbB 3 , and ErbB 4 , mediate signaling by EGF like polypeptides . ^^^ K721M alone exhibited no detectable signaling capacity , whereas coexpression of K721M with ErbB 2 , but not ErbB 3 or ErbB 4 , resulted in EGF dependent mitogen activated protein kinase ( MAPK ) activation . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Treatment of cells with EGF ( ErbB 1 specific ) or heregulin ( ErbB 4 specific ) resulted in a hierarchic transactivations of ErbB 2 and ErbB 3 dependent on GF binding specificity . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Expression of individual EGF receptor ( EGFR ) and ErbB 2 , ErbB 3 , or ErbB 4 receptors was detected in 72 . 2 , 44 . 5 , 56 . 3 , and 29 . 8 % of bladder cancer cases , respectively . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Neither EGF nor heregulin ( HRG ) induced c erbB2 / c erbB 3 receptor complexes in BT 474 . ^^^ However , SK BR 3 cells exhibited a high amount of c erbB2 / c erbB 3 heterodimers even without growth factor stimulation which could be elevated after prolonged EGF and HRG treatment . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Expression of herstatin , an autoinhibitor of HER 2 / neu , inhibits transactivation of HER 3 by HER 2 and blocks EGF activation of the EGF receptor . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Six out of 11 human pancreatic cancer cell lines expressed all four epidermal growth factor ( EGF ) receptor family members ( HER 1 ( EGF R ) , HER 2 , HER 3 , and HER 4 ) , including Panc 89 cells . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| HER 3 ( also known as c Erb b 3 ) is a type 1 receptor tyrosine kinase similar in sequence to the epidermal growth factor ( EGF ) receptor . ^^^ We show that the EGF like domain of heregulin ( hrg ) binds to domains 1 and 2 of HER 3 , in contrast to the EGF receptor , for which prior studies have shown that a construct consisting of domains 3 and portions of domain 4 binds EGF . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| However , neither the phosphorylation status nor the cellular localization of the EGF receptors , ErbB 3 or ErbB 4 , were affected in infected cells . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Seven pairs of primers for oncogenes most probably associated with the process of carcinogenesis in stomach including cyclin E , c erbB 3 , HGR , c met , TDGF / cripto , FGF 4 , and EGF were used for the construction of fluorescent multiplex RT PCR . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Activation of ErbB1 / EGFR is mainly triggered by epidermal growth factor ( EGF ) and other related ligands , while activation of ErbB 2 , ErbB 3 , and ErbB 4 receptors occurs by binding to another set of EGF like ligands termed neuregulins ( NRGs ) . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| The EFGR family is a group of four structurally similar tyrosine kinases ( EGFR , HER2 / neu , ErbB 3 , and ErbB 4 ) that dimerize on binding with a number of ligands , including EGF and transforming growth factor alpha . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Epidermal growth factor ( EGF ) and transforming growth factor ( TGF ) alpha are potent activators of the ErbB 1 receptor , but , unlike TGF alpha , EGF is also a weak activator of ErbB 2 / ErbB 3 heterodimers . ^^^ To understand the specificity of EGF like growth factors for binding to distinct ErbB members , we used EGF / TGF alpha chimeras to examine the requirements for ErbB 2 / ErbB 3 activation . ^^^ Here we show that in contrast to these two wild type ligands , distinct EGF / TGF alpha chimeras are potent activators of ErbB 2 / ErbB 3 heterodimers . ^^^ A chimera consisting of human EGF sequences with the linear N terminal region of human TGF alpha was found to be almost as potent as the natural ligand neuregulin ( NRG ) 1beta in activating 32D cells expressing ErbB 2 / ErbB 3 and human breast cancer cells . ^^^ Subsequent exchange studies revealed that introduction of both His 2 and Phe 3 into the linear N terminal region was already sufficient to make EGF a potent activator of ErbB 2 / ErbB 3 heterodimers , indicating that these two amino acids contribute positively to this receptor binding . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| In A 549 and H 322 cells , which express a detectable amount of EGF R ( ErbB 1 ) , ErbB 2 , ErbB 3 , and ErbB 4 receptors , the LA 1 mAb induces up regulation of the E cadherin / catenin complex ( alpha , beta , and gamma catenins ) . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Each member of the group formed by BTC , heparin binding EGF ( HB EGF ) and epiregulin ( EP ) can interact with both the EGF receptor ( EGFR ) and heregulin receptors ( ErbB 3 and ErbB 4 ) , and are hence called `` bispecific ' ' ligands . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| The neuregulin ( NRG ) / epidermal growth factor ( EGF ) family of growth factors consists of several ligands that specifically activate four erbB receptor tyrosine kinases , namely erbB 1 ( EGF R ) , erbB 2 ( neu ) , erbB 3 , and erbB 4 . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Here , we demonstrate that PP 1 , a selective inhibitor of Src family kinases , blocks the EGF induced Gab 2 tyrosine phosphorylation without inhibiting EGF induced phosphorylation of the EGF receptor , ErbB 3 , or Shc . ^^^ Mutation of the proline rich sequences in Gab 2 prevented EGF induced Gab 2 phosphorylation , PI 3 kinase / Akt activation , and DNA synthesis , demonstrating that Gab 2 phosphorylation is critical for EGF induced mitogenesis and is not complemented by ErbB 3 or Shc phosphorylation . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| In these cells , three major p 85 containing complexes were formed after EGF treatment : ErbB 3 p85 , Shc p 85 , and a multimeric Gab 2 Grb2 SHP 2 p85 , which accounted for more than 80 % of total EGF induced PI3K activity ( Kong , M . , C . ^^^ Here we show that , after EGF treatment , ErbB 3 p85 and Shc p 85 complexes were localized to plasma membrane and endosomes , whereas the multimeric Gab 2 Grb2 SHP 2 p85 complex was formed rapidly ( peak at 30 sec ) and exclusively in cytosol . ^^^ In summary , after EGF stimulation , ErbB 3 , Shc , and Gab 2 are differentially compartmentalized in rat liver , where they associate with and activate PI3K . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| While HER 1 ( EGF receptor ) and HER 2 have been extensively studied , little is known about the clinical significance of HER 3 and especially HER 4 expression . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| RT PCR analysis revealed that the EGF receptors erbB 1 , erbB 2 , erbB 3 and erbB 4 were expressed at all stages of the spermatogenic wave , whereas differential expression was found in isolated Leydig , Sertoli and peritubular cells . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| The EGF Receptor ( EGFR ) , the first transmembrane receptor tyrosine kinase cloned and sequenced , and its closely related family members HER 2 , HER 3 , and HER 4 , play myriad roles in mammalian growth and development . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Coimmunoprecipitation experiments also indicated that Grb 7 and NIK could be simultaneously recruited into signaling complexes of all known EGF / heregulin receptors , including EGFR , ErbB 2 , ErbB 3 , and ErbB 4 . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Inhibition of ErbB 2 and ErbB 3 expression by quercetin prevents transforming growth factor alpha ( TGF alpha ) and epidermal growth factor ( EGF ) induced human PC 3 prostate cancer cell proliferation . ^^^ Co treating PC 3 cells with quercetin significantly attenuated EGF and TGF alpha induced growth and phosphorylation of ErbB 2 , ErbB 3 , c Raf , MAPK kinase 1 / 2 ( MEK1 / 2 ) , MAPK , Elk 1 and Akt 1 . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| We have investigated the protein expression pattern of the four ErbB receptors EGFR , Her 2 / neu , Her 3 and Her 4 , and correlated it with their putative ligands EGF , TGF alpha and HRG in 74 women with invasive breast cancer . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| We characterized the expression of epidermal growth factor ( EGF ) like growth factors ( EGF , transforming growth factor alpha ( TGF alpha ) , heparin binding EGF like growth factor ( HB EGF ) , betacellulin ( BTC ) , amphiregulin ( APR ) , epiregulin ( EPR ) and neuregulin ( NRG ) 1 ) and erbB receptors ( EGF receptor ( EGFR ) , erbB2 / neu , erbB 3 and erbB 4 ) in the vaginae of mice treated either neonatally ( 0 4 day ) or as adults ( 55 59 day ) with estrogens . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| NTAK ( neural and thymus derived activator for ErbB kinases ) , also known as neuregulin 2 , is a member of the epidermal growth factor ( EGF ) family , which binds directly to ErbB 3 and ErbB 4 and transactivates ErbB 2 . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| On the basis of the 3D structures of the extracellular ligand binding domains of the epidermal growth factor ( EGF ) receptor ( EGFR ) and ErbB 3 , a mechanism has been proposed for how the extracellular region of the EGFR is maintained in an autoinhibited configuration and for how EGF binding induces EGFR dimerization and activation . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| When erbB 2 monomeric yellow fluorescent protein ( mYFP ) or erbB 3 monomeric Citrine ( mCitrine ) were coexpressed with erbB 1 , the rates and extent of endocytosis of EGF QD and the RTK VFP demonstrated that erbB 2 but not erbB 3 heterodimerizes with erbB 1 after EGF stimulation , thereby modulating EGF induced signaling . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Nrdp1 / FLRF is a RING finger containing ubiquitin ligase that catalyzes degradation of the EGF receptor family member , ErbB 3 . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Anti ErbB 2 monoclonal antibody 2C4 blocked heregulin stimulated phosphorylation of ErbB 2 and ErbB 3 ; activation of mitogen activated protein kinase ( MAPK ) , phosphatidylinositol 3 ' kinase ( PI3K ) , and Akt ; proliferation ; and anchorage independent growth . 2C4 blocked EGF mediated phosphorylation of ErbB 2 and inhibited PI3K / Akt and anchorage independent growth but did not affect ErbB 1 or MAPK . ^^^ Immunoprecipitations showed that ErbB 3 and Grb 2 associated binder ( Gab ) 1 were phosphorylated and associated with PI3K activity after heregulin treatment and that Gab 1 and Gab 2 , but not ErbB 3 , were phosphorylated and associated with PI3K activity after EGF treatment . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| EGF dependent activation of the Erk1 / 2 pathway and the upstream activators of Akt ( Gab 1 , erbB 3 , PI 3 kinase , and PDK 1 ) showed no density dependency . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Here we tested whether the related human LRIG 1 ( also called Lig 1 ) protein can act as a negative regulator of EGF receptor and its relatives , ErbB 2 , ErbB 3 , and ErbB 4 . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| However , expression levels for EGF receptor , erbB 2 and erbB 3 in specialized columnar epithelium ( SCE ) of Barrett ' s esophagus have yet to be determined . ^^^ To investigate the relationship between EGF family receptors and Barrett ' s esophagus , we examined expression levels for EGF receptor , erbB 2 and erbB 3 in SCE of Barrett ' s esophagus . ^^^ Biopsy samples of Barrett ' s mucosa stained or not stained with methylene blue applied endoscopically were used for histological evaluation and Western blot analysis of EGF receptor , erbB 2 and erbB 3 proteins . ^^^ In the Barrett ' s group , Western blot analysis showed that EGF receptor and erbB 2 were equally and strongly expressed in SCE and squamous epithelium ; in contrast , erbB 3 expression in SCE was considerably weaker . ^^^ Immunohistochemical analysis of SCE in Barrett ' s esophagus showed positive EGF receptor and erbB 2 expression , and no erbB 3 expression . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| In an effort to elucidate the role of ErbB receptors in human head and neck squamous cell carcinoma ( HNSCC ) , expression abnormalities and subcellular localization of epidermal growth factor receptor ( EGFR ) , ErbB 2 , ErbB 3 , and ErbB 4 were investigated along with EGF and tenascin by immunohistochemistry in 38 carcinomas as compared to adjacent normal mucosa of 24 cases . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Over the past year , the field has progressed from having a complete absence of 10 ray crystal structures to having eight such structures ; ErbB 2 alone or complexed with the two monoclonal antibodies pertuzumab ( Genentech Inc / Roche Holdings AG / Chugai Pharmaceutical Co Ltd ) and trastuzamab , ErbB 3 without a ligand , EGFR with a ligand bound in an unactivated monomeric conformation , and EGFR with either epidermal growth factor ( EGF ) or transforming growth factor alpha ( TGFalpha ) in a 2 : 2 dimeric complex . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| However , in cells expressing ErbB 3 , EGF and neuroregulin can stimulate PI 3 kinase and Akt activation in a Gab 1 dependent or Gab 1 independent manner . . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Since limited information exists concerning the expression of other parts of the EGF system , we examined the expression of the receptors HER 3 and HER 4 and their activating ligands , the heregulins ( HRGs ) , in bladder cancer patients . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| The ErbB family , ErbB 1 ( also known as the epidermal growth factor receptor EGFR ) , ErbB 2 , ErbB 3 , and ErbB 4 comprise a group of receptor tyrosine kinases that interact with ligands from the epidermal growth factor ( EGF ) superfamily , subsequently dimerize , catalytically activate each other by cross phosphorylation , and then stimulate various signaling pathways . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Mammals contain four members ( HER1 / EGFR , HER2 / Neu , HER 3 , and HER 4 ) of the epidermal growth factor receptor ( EGFR ) family , which transduce extracellular signals by EGF family peptide growth factors . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| The expression of EGF receptors ( ErbB 1 and ErbB 2 , but not ErbB 3 or ErbB 4 ) was down regulated significantly in the same forebrain regions . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Treatment of astrocytes , however , with heregulin , which signals through other members of the EGF receptor family ( ErbB 2 , ErbB 3 , ErbB 4 ) , did not induce CSPG upregulation . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Neuregulins constitute a family of EGF like ligands that bind to HER 3 or HER 4 , preferably forming heterodimers with the orphan receptor HER 2 . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Human EGF Receptor 1 ( HER 1 ) showed highest expression during the proliferative phase , HER 2 and HER 4 during the early and HER 3 during the late secretory phase . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Our results demonstrated that ( 1 ) EGFR kinase can efficiently phosphorylate a broad range of diverse peptide sequences representing ErbB sites ; ( 2 ) certain ErbB 2 , ErbB 3 and ErbB 4 sites had higher specificity constants than any EGFR sequence and ( 3 ) EGF stimulation consistently increases the k ( cat ) approx . 5 fold , but does not significantly alter the K ( m ) for any ErbB peptides . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| We studied epithelial expression of EGF , transforming growth factor a , amphiregulin , heregulin ( HRG ) , betacellulin ( BTC ) , and their receptors , EGFR , HER 2 , and HER 3 , by immunohistochemical analysis in resected bronchial tissue from 20 subjects with ( forced expiratory volume in 1 second [ FEV ( 1 ) ] < 75 % of predicted value ) and 18 without ( FEV ( 1 ) > 85 % predicted value ) COPD . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Six genes were over expressed ( ERBB 2 , TGFA , HB EGF , AREG , EREG and EPGN ) , 3 were under expressed ( ERBB 1 , ERBB 4 and NRG 4 ) and 2 were over or under expressed ( ERBB 3 and BTC ) . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Tumor endothelial cells express epidermal growth factor receptor ( EGFR ) but not ErbB 3 and are responsive to EGF and to EGFR kinase inhibitors . ^^^ On the other hand , normal endothelial cells did not respond to EGF but instead were responsive to neuregulin ( NRG ) , a ligand for ErbB 3 and ErbB 4 . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| In this study , we broadened the ErbB binding specificity of EGF by introducing the optimal sequence requirements for ErbB 3 binding in both the N and C terminal linear regions . ^^^ One such EGF mutant , designated WVR / EGF / IADIQ , gained high affinity for ErbB 3 and showed concomitant ErbB 3 activation through ErbB2 . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| Recently , a high expression of the two remaining receptors of the EGF system , HER 3 and HER 4 has been related to a favourable prognosis . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| EGF receptor ( EGFr ) expression declined in the first 24 h , whereas ErbB 3 expression rose . ^^^ Although ErbB 2 was not present in freshly isolated hepatocytes , EGF and insulin independently induced ErbB 2 while suppressing ErbB 3 expression . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| When stimulated with only the EGF like domain , ERBB 3 shows degradation rates comparable with constitutive turnover , but stimulation with full length neuregulin 1 resulted in receptor degradation at rates that are comparable with activated EGF receptor . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| EGF strongly stimulates p 42 to bind ErbB 3 , and the association depends on PKC mediated phosphorylation of Ebp 1 . ^^^ By contrast , p 48 does not bind to ErbB 3 regardless of EGF treatment . ^^^ |
|
| Interacting proteins: P01133 and P21860 |
Pubmed |
SVM Score :0.0 |
| To go further in the characterization of the EGF pathway , we screened EGFR , ERBB 2 , ERBB 3 , KRAS , BRAF , and PIK3CA for mutations in 2 groups of White patients with nonsmall cell lung cancer ( 45 cancers from women and 46 cancers from men ) . ^^^ |
|