| Interacting proteins: P14384 and P01133 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P14384 and P01133 |
Pubmed |
SVM Score :0.0 |
| Approximately 5 ng [ 125I ] iodo EGF ( approximately 10 ( 6 ) cpm ) were injected 4 into day 10 , day 13 , or day 17 pregnant CD 1 mice ; radioactivity in plasma , placentas , and conceptuses was measured up to 2 h after injection . ^^^ |
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| Interacting proteins: P14384 and P01133 |
Pubmed |
SVM Score :0.0 |
| The cells were grown in monolayer culture in replicate wells ; 40 , 000 50 , 000 cpm 125I labeled mouse EGF with a specific activity of 100 120 microCi / micrograms was added to each well . ^^^ |
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| Interacting proteins: P14384 and P01133 |
Pubmed |
SVM Score :0.0 |
| CPM was also found in soluble form in biological fluids such as urine and amniotic fluid where it is the primary enzyme that hydrolyzes epidermal growth factor ( EGF ) , producing des Arg 53 EGF . ^^^ |
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| Interacting proteins: P14384 and P01133 |
Pubmed |
SVM Score :0.0 |
| Results of dose response experiments revealed that EGF had a maximum fibroblast stimulation at 66078 + / 1979 counts per minute ( cpm ) , with peak counts on day 6 of culture , as measured by [ 3H ] thymidine incorporation . ^^^ |
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| Interacting proteins: P14384 and P01133 |
Pubmed |
SVM Score :0.0 |
| The results show that EGF ( 10 ng / ml ) significantly stimulates the incorporation of [ 3H ] thymidine in the SK N SH cells only in the presence of fetal bovine serum ( FBS ) ( control = 58 , 285 + / 9327 cpm ; EGF = 75 , 523 + / 4457 , p < 0 . 05 ) . ^^^ |
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| Interacting proteins: P14384 and P01133 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Biological activity assays of EGF ( 1 53 ) , EGF ( 1 52 ) , and EGF ( 1 51 ) gave almost identical thymidine uptake dose response curves ( maximal responses increasing baseline uptake from 4400 ( 600 ) cpm ( mean ( SEM ) ) to about 22 000 ( 2000 ) cpm when EGF was added at 1 . 6 nM ) . ^^^ |
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| Interacting proteins: P14384 and P01133 |
Pubmed |
SVM Score :0.0 |
| Extracellular conversion of epidermal growth factor ( EGF ) to des Arg 53 EGF by carboxypeptidase M . ^^^ In this study , purified carboxypeptidase M efficiently released the COOH terminal arginine residue from EGF with a Km = 56 microM , kcat = 388 min 1 , and kcat / Km = 6 . 9 microM 1 min 1 . ^^^ This conversion was blocked by immunoprecipitation with specific antiserum to carboxypeptidase M or by 10 microM DL 2 mercaptomethyl 3 guanidinoethylthiopropanoic acid ( a carboxypeptidase M inhibitor ) , indicating that the major EGF metabolizing enzyme in these fluids is carboxypeptidase M . ^^^ When incubated on a confluent monolayer of Madin Darby canine kidney ( MDCK ) cells , EGF was readily converted to a single metabolite , des Arg 53 EGF , by carboxypeptidase M . ^^^ In addition , inhibition of the conversion of EGF to des Arg 53 EGF by the carboxypeptidase M inhibitor did not affect the mitogenic potency of EGF . ^^^ |
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| Interacting proteins: P14384 and P01133 |
Pubmed |
SVM Score :0.0 |
| After incubation with 125I EGF , heparin treated cells also released significantly more cpm of 125I into EGF free medium ( 2526 + / 68 cpm H ; 903 + / 32 C ) . ^^^ |
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| Interacting proteins: P14384 and P01133 |
Pubmed |
SVM Score :0.0 |
| RESULTS : WB F 344 cells showed a proliferative response to the cytokines of hepatocyte growth factor ( HGF ) , epidermal growth factor ( EGF ) , fibroblast growth factor ( FGF ) , Insulin at the dose of 80 ng / ml , and the relative cpm values are 982 . 95 , 906 . 32 , 863 . 98 and 968 . 67 respectively , while non response to interleukin 6 ( IL 6 ) , tumor necrosis factor alpha ( TNF alpha ) at the same dose , and an inhibition or apoptosis response to transforming growth factor beta ( TGF beta ) at 80 ng / ml with a 26 . 89 % apoptotic rate . ^^^ |
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| Interacting proteins: P14384 and P01133 |
Pubmed |
SVM Score :0.0 |
| Differentially expressed genes were screened by subtractive hybridization . ( 3 ) H thymidine uptake was significantly increased by EGF with time ( untreated vs . 24 h treated , untreated vs . 48 h treated : 305 . 5 + / 3 . 5 cpm vs . 380 . 3 + / 17 . 3 cpm ( P < 0 . 05 ) , 309 . 2 + / 4 . 51 vs . 929 + / 9 . 19 cpm , ( P < 0 . 005 ) , respectively ) . ^^^ |
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