| Interacting proteins: Q13105 and P01106 |
Pubmed |
SVM Score :0.70084203 |
| Association of Myc with the zinc finger protein Miz 1 defines a novel pathway for gene regulation by Myc . 0.70084203^^^ |
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| Interacting proteins: Q13105 and P01106 |
Pubmed |
SVM Score :0.50801302 |
| Orian and Eisenman discuss how Myc interacts with Miz 1 to block the expression of a cell cycle inhibitory protein , p 15 ( INK4b ) , and how TGF beta is able to unblock Myc dependent repression of Miz 1 . . 0.50801302^^^ |
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| Interacting proteins: Q13105 and P01106 |
Pubmed |
SVM Score :1.116786 |
| Conversely , HCF 1 interacted with two separate regions in Miz 1 : the N terminal POZ domain and a C terminal domain ( residues 637 803 ) previously shown to harbor determinants for interaction with c Myc and the coactivator p 300 . 1.116786^^^ |
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| Interacting proteins: Q13105 and P01106 |
Pubmed |
SVM Score :0.8582004 |
| Our results demonstrate that the c Myc responsive region is situated in the p 21 core promoter . c Myc binds to this region in vitro and in vivo through interaction with the initiator binding Zn finger transcription factor Miz 1 , which associates directly with the promoter . 0.8582004^^^ Using mutants of c Myc with impaired binding to Miz 1 , our results further show that repression of p 21 promoter / reporters as well as the endogenous p 21 gene by Myc depends on interaction with Miz 1 . 0.55742747^^^ |
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| Interacting proteins: Q13105 and P01106 |
Pubmed |
SVM Score :0.7675448 |
| Our results further indicate that p 300 can acetylate DNA bound Myc : Max complexes and that acetylated Myc : Max heterodimers efficiently interact with Miz 1 . . 0.7675448^^^ |
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| Interacting proteins: Q13105 and P01106 |
Pubmed |
SVM Score :0.0 |
| Using yeast two hybrid screen we identified Myc Interacting Zinc finger protein 1 ( Miz 1 ) as new interacting partner . ^^^ |
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| Interacting proteins: Q13105 and P01106 |
Pubmed |
SVM Score :0.0 |
| Two hybrid cloning identifies a novel POZ domain Zn finger protein ( Miz 1 ; Myc interacting Zn finger protein 1 ) that specifically interacts with Myc , but not with Max or USF . ^^^ Binding of Myc to Miz 1 requires the helix loop helix domain of Myc and a short amphipathic helix located in the carboxy terminus of Miz 1 . ^^^ Expression of Myc inhibits transactivation , overcomes Miz 1 induced growth arrest and renders Miz 1 insoluble in vivo . ^^^ These processes depend on Myc and Miz 1 association and on the integrity of the POZ domain of Miz 1 , suggesting that Myc binding activates a latent inhibitory function of this domain . ^^^ Fusion of a nuclear localization signal induces efficient nuclear transport of Miz 1 and impairs the ability of Myc to overcome transcriptional activation and growth arrest by Miz 1 . ^^^ |
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| Interacting proteins: Q13105 and P01106 |
Pubmed |
SVM Score :0.0 |
| We show that Myc is destroyed by ubiquitin mediated proteolysis , and define two elements in Myc that oppositely regulate its stability : a transcriptional activation domain that promotes Myc destruction , and a region required for association with the POZ domain protein Miz 1 that stabilizes Myc . ^^^ |
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| Interacting proteins: Q13105 and P01106 |
Pubmed |
SVM Score :0.0 |
| Recently described interactions between the CTD and other cellular proteins , including YY 1 , AP 2 , BRCA 1 , TFII 1 , and Miz 1 , suggest levels of regulatory complexity beyond Max in controlling DNA recognition by c Myc . ^^^ |
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| Interacting proteins: Q13105 and P01106 |
Pubmed |
SVM Score :0.0 |
| A number of interaction partners of Myc have been identified , such as Max , p 107 , TBP , YY 1 , Miz 1 , AP 2 and Nmi . ^^^ |
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| Interacting proteins: Q13105 and P01106 |
Pubmed |
SVM Score :0.0 |
| Moreover , quiescence and senescence can be distinguished by increased expression of Irlb , c Myc transcription factor , and Miz 1 , c Myc interacting Zn finger protein 1 , only in the former state . ^^^ |
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| Interacting proteins: Q13105 and P01106 |
Pubmed |
SVM Score :0.0 |
| Co transfections with the c Myc interacting zinc finger protein ( Miz 1 ) show that Miz 1 can overcome c Myc repression of Nramp 1 , and , from a deletion construct lacking E box sites , Miz 1 activates the Nramp 1 promoter . ^^^ |
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| Interacting proteins: Q13105 and P01106 |
Pubmed |
SVM Score :0.0 |
| Myc is directly recruited to the p 21 ( Cip 1 ) promoter by the DNA binding protein Miz 1 . ^^^ |
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| Interacting proteins: Q13105 and P01106 |
Pubmed |
SVM Score :0.0 |
| Using both c myc ( / ) cells and a point mutant of Myc that is deficient in Miz 1 dependent repression , we show that Myc negatively regulates transcription of p21Cip1 upon UV irradiation and facilitates recovery from UV induced cell cycle arrest through binding to Miz 1 . ^^^ Negative regulation of the mammalian UV response by Myc through association with Miz 1 . ^^^ The Myc oncoprotein represses initiator dependent transcription through the POZ domain transcription factor Miz 1 . ^^^ |
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| Interacting proteins: Q13105 and P01106 |
Pubmed |
SVM Score :0.0 |
| Mad 4 is regulated by a transcriptional repressor complex that contains Miz 1 and c Myc . ^^^ We demonstrate that the initiator element is required for Mad 4 activation , and show that induction is associated with the loss from the initiator of a complex that contains Miz 1 and c Myc . ^^^ Miz 1 activates the Mad 4 promoter in transient transfection assays , and this effect is antagonized by c Myc . ^^^ These data suggest that the expression of Mad 4 in proliferating undifferentiated cells is suppressed by the binding of a c Myc Miz 1 repressor complex at the initiator , and that the activation of Mad 4 during differentiation results , at least in part , from a decrease in c Myc mediated repression . . ^^^ |
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| Interacting proteins: Q13105 and P01106 |
Pubmed |
SVM Score :0.0 |
| One mechanism is limited to the binding of Myc Max heterodimers to the Inr element in their promoters and inhibition of Miz 1 or other transcriptional activators via the C terminal domain of c Myc . ^^^ |
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| Interacting proteins: Q13105 and P01106 |
Pubmed |
SVM Score :0.0 |
| Recent evidence suggests that complex formation by Myc with a zinc finger transcription factor , Miz 1 , plays an important role in mediating repression by Myc . ^^^ |
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| Interacting proteins: Q13105 and P01106 |
Pubmed |
SVM Score :0.0 |
| Repression via c Myc occurs at the initiator elements , whereas a c Myc interacting protein ( Miz 1 ) stimulates transcription . ^^^ Repression occurs by c Myc competing with p300 / CBP for binding Miz 1 . ^^^ Miz 1 directed RNA interference confirms a stimulatory role for Miz 1 in Nramp 1 promoter function . c Myc , Miz 1 , and Sp 1 were identified as binding to the Nramp 1 core promoter in control cells and following acute stimulation with interferon gamma and lipopolysaccharide . ^^^ |
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| Interacting proteins: Q13105 and P01106 |
Pubmed |
SVM Score :0.0 |
| In vivo , Miz 1 forms a complex with the Myc oncoprotein and recruits Myc to core promoter elements . ^^^ Myc represses transcription through Miz 1 binding sites . ^^^ |
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| Interacting proteins: Q13105 and P01106 |
Pubmed |
SVM Score :0.0 |
| Nramp 1 expression is repressed and activated by the proto oncogene c Myc and Miz 1 ( c Myc interacting zinc finger protein 1 ) respectively . ^^^ Here we demonstrate , using a c Myc mutant ( V394D , Val ( 394 ) > Asp ) that is incapable of binding Miz 1 , that c Myc repression of Nramp 1 transcription is dependent on its interaction with Miz 1 . ^^^ An oligo pull down assay demonstrates specific binding of recombinant Miz 1 to the Nramp 1 Miz 1 binding site or initiator element ( s ) , and Miz 1 dependent c Myc recruitment . . ^^^ |
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| Interacting proteins: Q13105 and P01106 |
Pubmed |
SVM Score :0.0 |
| Moreover , the effect was independent of Miz 1 binding to c Myc . ^^^ |
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| Interacting proteins: Q13105 and P01106 |
Pubmed |
SVM Score :0.0 |
| Miz 1 has two functions in response to DNA damage : first , it is required for upregulation of a large group of genes , a function that is regulated by c Myc , but not by 14 3 3eta ; second , Miz 1 represses the expression of many genes in response to DNA damage in an Akt and 14 3 3eta regulated manner . . ^^^ |
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| Interacting proteins: Q13105 and P01106 |
Pubmed |
SVM Score :0.0 |
| Myc is known to be recruited to the p21Cip1 promoter by the DNA binding factor Miz 1 . ^^^ Consistent with this , we observe that Myc and Dnmt3a form a ternary complex with Miz 1 and that this complex can corepress the p21Cip1 promoter . ^^^ |
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| Interacting proteins: Q13105 and P01106 |
Pubmed |
SVM Score :0.0 |
| As an example , MYC regulates the MYC interacting zinc finger protein 1 ( MIZ 1 ) , which is able to inhibit the expression of the indirect target p21WAF1 . ^^^ |
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| Interacting proteins: Q13105 and P01106 |
Pubmed |
SVM Score :0.0 |
| Pontin and Reptin regulate cell proliferation in early Xenopus embryos in collaboration with c Myc and Miz 1 . ^^^ The N termini of xPontin and xReptin , which mediate the mitogenic effect were mapped to contain c Myc interaction domains . c Myc protein promotes cell cycle progression either by transcriptional activation through the c Myc / Max complex or by repression of cyclin dependent kinase inhibitors ( p 21 , p 15 ) through c Myc / Miz 1 interaction . ^^^ Importantly , xPontin and xReptin exert their mitogenic effect through the c Myc / Miz 1 pathway as dominant negative Miz 1 and wild type c Myc but not a c Myc mutant deficient in Miz 1 binding could rescue embryonic lethality . ^^^ We propose a novel function of xPontin and xReptin as co repressors in the c Myc / Miz 1 pathway . . ^^^ |
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| Interacting proteins: Q13105 and P01106 |
Pubmed |
SVM Score :0.0 |
| In reporter assays , Miz 1 enhances reporter GFP expression ; this enhancement is inhibited by co expressing c Myc . ^^^ Thus hMad 4 , as does its murine counterpart , contains the Inr element through which Miz 1 activates its expression ; but this action is inhibited in the presence of c Myc . ^^^ Our results suggest that : ( 1 ) replicative senescence specific factors may block c Myc inhibition of Miz 1 activation of hMad 4 expression ; and ( 2 ) the continual presence of hMad 4 protein may transcriptionally repress selected c Myc target genes , whose functions are key to the signaling pathways leading to apoptosis inhibition and permanent exit of cell cycle traverse in normal human fibroblasts . . ^^^ |
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| Interacting proteins: Q13105 and P01106 |
Pubmed |
SVM Score :0.0 |
| The Myc oncoprotein forms a binary activating complex with its partner protein , Max , and a ternary repressive complex that , in addition to Max , contains the zinc finger protein Miz 1 . ^^^ |
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| Interacting proteins: Q13105 and P01106 |
Pubmed |
SVM Score :0.0 |
| For many of the repressed target genes , down regulation by c Myc relies on its ability to bind and inactivate the transcription factor Miz 1 . ^^^ Although Miz 1 inactivation is suspected to be essential for at least some of the biological activities of c Myc , it has been difficult to demonstrate this requirement experimentally . ^^^ Using a combination of short hairpin RNA mediated knockdown and a previously characterized mutant of c Myc that is defective for Miz 1 inactivation , we examined whether this inactivation is critical for three of the most central biological functions of c Myc , cell cycle progression , transformation , and apoptosis . ^^^ The results of this analysis demonstrated that in the in vitro assays utilized here , Miz 1 inactivation is dispensable for c Myc induced cell cycle progression and transformation . ^^^ In marked contrast , the ability of c Myc to induce apoptosis in primary diploid human fibroblasts in response to growth factor withdrawal is entirely dependent on its ability to inactivate Miz 1 . ^^^ |
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| Interacting proteins: Q13105 and P01106 |
Pubmed |
SVM Score :0.0 |
| Myc regulates keratinocyte adhesion and differentiation via complex formation with Miz 1 . ^^^ We show that Miz 1 , which mediates Myc repression of gene expression , is expressed in the epidermal basal layer . ^^^ A large percentage of genes regulated by the Myc Miz 1 complex in keratinocytes encode proteins involved in cell adhesion , and some , including the alpha 6 and beta 1 integrins , are directly bound by Myc and Miz 1 in vivo . ^^^ Using a Myc mutant deficient in Miz 1 binding ( MycV394D ) , we show that Miz 1 is required for the effects of Myc on keratinocyte responsiveness to TGF beta . ^^^ In reconstituted epidermis , Myc induces differentiation and loss of cell polarization in a Miz 1 dependent manner . ^^^ |
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| Interacting proteins: Q13105 and P01106 |
Pubmed |
SVM Score :0.0 |
| Experiments using different p21Cip1 promoter mutants showed that transcriptional repression of p21Cip1 by c Myc was mediated through Miz 1 and Sp 1 binding sites within the proximal region of the p21Cip1 promoter in normal IECs . ^^^ |
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| Interacting proteins: Q13105 and P01106 |
Pubmed |
SVM Score :0.0 |
| The assay revealed that HMGCS 2 is a direct target of c Myc , which represses HMGCS 2 transcriptional activity . c Myc transrepression is mediated by blockade of the transactivating activity of Miz 1 , which occurs mainly through a Sp 1 binding site in the proximal promoter of the gene . ^^^ |
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