| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| However , the induction of fos , jun , and myc by EGF and TPA was not significantly inhibited in this cell line . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| PRL , but not A 23187 or TPA , stimulated c myc gene expression in quiescent Nb 2 cells . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Phorbol ester ( TPA ) increased the binding activity of AP 1 and c Myc , as expected . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| A time course study showed that both hKLK 2 and c myc mRNAs were repressed by TPA as early as four hours . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| In contrast , TPA treatment inhibited the expression of c myc mRNA . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| However , we do not observe an increase in Ser 62 phosphorylation in endogenous c Myc after TPA treatment of cells . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Overexpression of c myc was inhibited by TPA and p21waf1 / cip1 mRNA increased . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| In this study , the c myc message of B cells or B cell lines stimulated by 12 o tetradecanoylphorbol 13 acetate ( TPA ) , A 23187 , Staphylococcus aureus Cowan 1 ( SAC ) , or anti mu was not expressed or was poorly expressed in common variable immunodeficiency ( CVID ) patients whose B cells did not differentiate or only poorly differentiated to SAC plus recombinant interleukin 2 , whereas the c myc message of 1 CVID patient ' s B cells that differentiated well in IgM secretion to SAC plus recombinant interleukin 2 was well expressed when stimulated by TPA , A 23187 , SAC , or anti mu . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| The effects of staurosporine were comparable to those induced by the protein kinase C ( PKC ) activator , 12 O tetradecanoyl phorbol 13 acetate ( TPA ) , with respect to induction of neuronal differentiation , i . e . neurite outgrowth , inhibition of DNA synthesis , induction and down regulation of c myc protein expression , induction of mRNA for both neuropeptide Y ( NPY ) and growth associated protein 43 ( GAP 43 ) and stimulation of tyrosine hydroxylase expression . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Further , the differentiation study with TPA and RA revealed that the growth , S phase and also c myc protein expression were suppressed during the differentiation . . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Thus , treatment of cells with TPA results in a reduction in the levels of c myb and c myc mRNA , while the expression of c fos , c jun , and junB is greatly enhanced . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| In addition , constitutive expression of exogenous myc inhibited induced differentiation of these cells by a variety of treatments including addition to the medium of lipopolysaccharide ( LPS ) or the phorbol ester 12 O tetradecanoyl phorbol 13 acetate ( TPA ) as well as complete withdrawal of serum from the medium . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| The down regulation of the differentiation related oncogenes c myc and c myb was the same in cells treated with TPA in the presence or absence of glucocorticoids . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| The modulation of the c fos and c myc expression has been studied by Northern analysis , 15 , 30 and 60 minutes after treatment of the P388D1 cells by the phorbol ester ( TPA ) , the Calcium ionophore A 23187 ( Ca2+I ) , the N acetyl muramyldipeptide ( MDP ) or the macrophage Colony Stimulating Factor ( CSF 1 ) . ^^^ The highest modulation of c fos and c myc was observed with TPA . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Northern blotting showed that mRNA levels for the four growth related genes transforming growth factor beta 1 ( TGF beta 1 ) , transforming growth factor beta 3 ( TGF beta 3 ) , tissue type plasminogen activator ( tPA ) , and c myc were significantly elevated in clonal mouse prostate carcinomas grown in castrated hosts . ^^^ The response of TGF beta 1 , tPA , and c myc mRNA levels in the carcinomas grown in castrated hosts was similar to that shown previously in normal rat ventral prostate . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| In vivo treatment with the phorbol ester TPA rapidly elevates uterine levels of fos , jun , and myc transcripts , indicating that expression of these protooncogenes is under non estrogenic as well as estrogenic regulation in this target tissue . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| This conclusion derives from the observation that c jun expression mediated via protein kinase C activation with phorbol ester ( 12 0 tetra decanoylphorbol 13 acetate , TPA ) treatment does not lead to c myc expression in parent FDC P 1 cells . ^^^ On the contrary , FDC P 1 cells stably transfected with a c myc gene controlled under the influence of a metallothionein IIA promoter ( containing the TPA responsive element [ TRE ] ) express the transfected MTIIA c myc and downregulate the endogenous c myc in response to protein kinase C activation with TPA . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Using the phorbol ester 12 O tetradecanoyl phorbol 13 acetate ( TPA ) and calcium , two agents known to induce keratinocyte differentiation in vitro , we examined the expression of the genes encoding c fos , c myc , and c jun ; involucrin , a protein precursor of the keratinocyte cornified envelope ; and L 7 , a ribosomal protein . ^^^ The c myc proto oncogene , known to have a high constitutive expression in actively proliferating cells , was strongly downregulated by TPA , but calcium had no effect over a 32 hour period , consistent with the greater growth inhibition of TPA in this system . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Treatment of quiescent MG 63 cells with 12 O tetradecanoylphorbol 13 acetate ( TPA ) or platelet derived growth factor ( PDGF ) stimulates the rapid accumulation of c myc RNA . ^^^ We had previously shown that PDGF action is not affected by prior treatment of MG 63 cells with TPA , a treatment which desensitizes the c myc response to TPA . ^^^ Similarly , TPA pretreatment had minimal effect on forskolin or IBMX induced c myc expression . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Exposure of THP 1 to TNF alpha , IL 6 and TPA was associated with a rapid downregulation of c myc expression , that returned to starting levels within 36 h . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Furthermore , KG 1a cells responded to TNF , but not to TPA , with a partial down regulation of c myc mRNA levels and induction of M CSF gene transcription . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| The expression of the protein products of the c myc oncogene and retinoblastoma susceptibility gene ( RB ) was investigated during either goat anti mouse immunoglobulin ( GaMIg ) or phorbol ester ( TPA ) induced growth arrest of the murine B lymphoma cell line WEHI 231 . ^^^ The myc protein synthesized following 1 2 h of anti immunoglobulin or TPA treatment migrates more slowly in a polyacrylamide gel as a result of increased phosphorylation . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Previous work has suggested that the activation of protein kinase C by TPA contributes to the decrease in c myc expression during differentiation of these cells . ^^^ The present studies demonstrate that the decline in c myc mRNA levels following exposure of HL 60 cells to TPA is preceded by an increase in expression of this gene . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| On the other hand , the protein kinase C agonist , TPA , strongly activated c jun expression but poorly promoted expression ( transcription ) of c myc in FDC P 1 . ^^^ However , stable transfection of FDC P 1 with a c myc expression vector driven by a human methallothionein IIA promoter containing the TPA responsive element ( TRE ) , led to a cell clone , FDMT myc . ^^^ A1 , in which TPA mediated selective transcription of the transfected TRE driven c myc vector and down regulated expression of the endogenous c myc gene . ^^^ Augmented TPA dependent vector derived c myc expression was accompanied by enhanced mitogenesis of the cell line FDMT myc . ^^^ In addition , TPA mediated expression of the transfected c myc gene in FDMT myc . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Progression also did not involve a change in the RNA transcription rate of a number of cellular and viral genes , including actin , c Ha ras , c myc , 5 fos , erbB , TGF alpha , TGF beta , Pro 2 , transin , TPA R 1 , 5 myb and c mos , or other adenovirus genes in addition to E1A and E1B , including E2A and E 4 . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| In unstimulated proliferating JURKAT cells , high levels of C MYC , N RAS , and BCL 2 mRNAs were found that diminished rapidly following TPA induced cessation of growth . ^^^ Despite sustained expression of C MYC , BCL 2 , or the combination of these protooncogenes , TPA continued to inhibit JURKAT cell growth and to induce IL2R expression . ^^^ Thus , although C MYC and BCL 2 proto oncogene expression correlated with proliferation in TPA treated JURKAT cells , continuous over expression of even the combination of these oncogenes was insufficient for abrogating the effects of TPA in these leukemic T cells . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| After TPA and EGF , the time courses of stimulation of c fos and c myc were the same as those for mitogenically stimulated fibroblasts . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Treatment of these cells with phorbol 12 O tetradecanoate 13 acetate ( TPA ) , insulin and concanavalin A ( Con A ) resulted in transient accumulation of c myc transcripts within 2 hours . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Treatment of the SBW 1 cells with 5 azacytidine , TPA or forskolin did not release the downregulated expression of the rearranged c myc , suggesting no causative involvement of DNA methylation or protein phosphorylation in the downregulation . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Furthermore , down regulation of the rearranged c myc in the hybrid was also not restored by treatment with 5 azacytidine ( 5 AzaC ) , 12 O tetradecanoylphorbol 13 acetate ( TPA ) or forskolin , suggesting no causative involvement of DNA methylation or protein phosphorylation in down regulation . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| C myc mRNa is detected for up to 36 h after tape stripping , but only for the first hour after TPA application . ^^^ C myc mRNa is detected for up to 36 h after tape stripping , but only for the first hour after TPA application . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| In contrast to calcium , TPA induced differentiation is accompanied by dramatic changes in proto oncogene expression : marked c fos induction and considerable although transient decrease in c myc expression . ^^^ These effects might be important for the keratinocyte response to TPA : TPA treatment of a keratinocyte cell line ( RBK ) resistant to this substance has no effect on c myc expression and leads only to minimal c fos induction . ^^^ Expression of the myc and fos genes has been monitored in mouse primary keratinocytes after induction of terminal differentiation by calcium or tetradecanoylphorbol acetate ( TPA ) . myc RNA levels in growing cells are very high and remain elevated even at late times after calcium induced differentiation . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Treatment of quiescent cells with 12 O tetradecanoyl phorbol 13 acetate ( TPA ) to down regulate protein kinase C inhibited TPA stimulated c myc expression but did not affect the PDGF modulated process . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Induction of other TPA responsive genes , such as metallothionein , was not altered in some ras transformed cells ; c myc and c jun expression was constitutively high in all the ras transformed clones . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| The relative abundance of c myc mRNA was not affected , although a persistent inhibition of cellular proliferation occurred upon chronic exposure to TPA . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Reduced induction of c fos but not of c myc expressions in a nontumorigenic revertant R 1 of Ej ras transformed NIH / 3T3 cells treated with 12 O tetradecanoylphorbol 13 acetate ( TPA ) . ^^^ It has been reported that both c fos and c myc mRNAs are induced in NIH / 3T3 cells after 12 O tetradecanoylphorbol 13 acetate ( TPA ) treatment . ^^^ We have studied the effect of TPA on the expression of c fos and c myc in EJ ras transformed NIH / 3T3 and its nontumorigenic flat revertant R 1 cells . ^^^ Although TPA treatment induces c myc mRNA , as in the case of NIH / 3T3 cells , the induced level of c fos mRNA is greatly reduced not only in slow growing EJ ras transformed NIH / 3T3 but also in quiescent R 1 cells . ^^^ These observations suggest that the pathway from TPA to c fos gene is different from that to c myc gene and that the former pathway is down regulated in association not with the transformed phenotype , but with EJ ras expression , and it is possible that this reduced induction of c fos is not specific to TPA . . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| We have examined the restriction digest patterns of CCGG sequences in Kiras , Ha ras , and c myc oncogenes in rat tracheal epithelial cells transformed in vitro by 7 , 12 dimethylbenz ( a ) anthracene , benzo ( a ) pyrene / 12 O tetradecanoylphorbol 13 acetate ( TPA ) , or TPA alone . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Northern blot analyses demonstrated that TPA induced increased levels of fos , myc , phorbin , and ODC RNAs in control K16MV7 and K22MV7 cells , with maximum induction occurring at about 0 . 5 , 1 , 8 , and 8 h , respectively . ^^^ In K16PKC 4 and K22PKC 2 cells , TPA induction of phorbin and ODC RNAs was markedly enhanced , but this was not the case for myc and fos RNAs . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| When the phorbol ester 12 O tetradecanoyl phorbol 13 acetate ( TPA ) or serum is added to cultures of U 1690 cells the Mr 66 , 000 polypeptide is rapidly enriched while the Mr 60 , 000 form is decreased in the L myc immunoprecipitates . ^^^ The TPA induced phosphorylation of the L myc protein occurs in a protein synthesis independent manner as it is not inhibited by cycloheximide or anisomycin . ^^^ These data indicate that the phosphorylation of the L myc nuclear oncoprotein is modulated in response to TPA via a rapid signal transduction system involving protein kinase C . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Here we demonstrate that the normal c myc allele can be induced in BL cells by 12 O tetradecanoylphorbol 13 acetate ( TPA ) . ^^^ TPA did activate the normal c myc alleles in Raji ( P 207 ) , BL 36 , P3HR1 , Jijoye and LY 91 cells , but not in Raji ( DE 88 ) , BL 41 , BL 67 , LY 47 and KK 124 cells . ^^^ C myc RNA derived from the normal allele appeared 6 h after treatment with TPA and showed the characteristic preferential usage of the second promoter . ^^^ Despite the differences in c myc induction in Raji ( P 207 ) and Raji ( DE 88 ) cells , c fos and the early Epstein Barr virus gene DR were induced to a similar extent and with similar kinetics by TPA . ^^^ Expression of the translocated c myc alleles was also affected by TPA ; however , only if cycloheximide was simultaneously present . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| In addition , EGF , insulin , and TPA , like hCG , elevated mRNA levels of competence oncogenes ( c fos and c myc ) , albeit to different extents . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| The proto oncogenes jun B , c fos , and to a lesser extent jun D were stimulated by increasing the intracellular concentration of cAMP , whereas the TPA stimulation of c jun and c myc was inhibited under these conditions . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Furthermore , the treatment of transfected cells with phorbol ester ( TPA ) revealed that the polyadenylated CAT mRNAs form some genes were stabilized , in contrast , the mRNAs bearing 3 ' end structure of histone or C myc genes were not . . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| In this paper , we show that exposing phorbol ester sensitive ( S ) HL 60 cells to TPA caused the disappearance of the c myc protein antigen ( detected on Western blots ) in 4 h , whereas TPA had no effect on the c myc protein content of PET cells . ^^^ TPA induced the down regulation of c myc mRNA in S cells to a greater extent than in PET cells . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| The PKC activator tetradecanoyl phorbol acetate ( TPA ) alone induced a time and concentration dependent stimulation of the incorporation of [ 3H ] thymidine into the DNA of quiescent FRTL 5 cells , an effect anteceded by an increase in the levels of the mRNAs of the proto oncogene c myc and associated with a stimulation of cell replication . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| In these basal conditions , the individual addition of TSH , insulin , insulin like growth factor 1 ( IGF 1 ) , phorbol 12 myristate 13 acetate ( TPA ) , alpha 1 adrenergic agents , or A 23187 , increase c myc and / or c fos proto oncogene expression . ^^^ The sum of these results indicate that at least four separate signal systems independently increase c myc or c fos gene expression in FRTL 5 cells cAMP ( TSH ) , C kinase ( TPA ) , Ca++ / phosphoinositide ( A 23187 ) , and that influenced by insulin / IGF 1 . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Nuclear run on assays showed that E 1 transcription , as well as transcription of c fos , c myc , and beta actin was stimulated in 293 cells within 30 min of TPA exposure and returned to basal levels by 60 min . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| We show that the plant lectin concanavalin A ( ConA ) , the phorbol ester 12 O tetradecanoyl phorbol 13 acetate ( TPA ) and the Ca2+ ionophore A 23187 each causes a rapid increase in both c fos and c myc mRNAs . ^^^ Exogenous 8 bromo cyclic AMP ( but not 8 bromo cyclic GMP ) inhibits the c myc responses to ConA and TPA . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| To elucidate the early events of lymphocyte activation , we determined ( by Northern blot analysis ) the steady state levels of c fos and c myc mRNA in normal human peripheral blood lymphocytes ( PBL ) stimulated with the Ca2+ ionophore A 23187 and / or 12 O tetradecanoylphorbol 13 acetate ( TPA ) , whose biological activities are well defined . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| After stimulation of human lymphocytes by 12 0 tetradecanoyl phorbol acetate ( TPA ) and by the calcium ionophore A 23187 an early transient expression of proto oncogene fos followed by an expression of c myc is observed , as has been described in rodent fibroblast stimulated by growth factors . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| The phorbol ester TPA and the adenylate cyclase activator forskolin reduced the c myc RNA , levels and after 3 days of treatment a proportion of the cells accumulated in G 1 . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| The tumor promoter tetradecanoyl phorbol acetate ( TPA ) was found to selectively induce the transcription of c myc without observable effect on the transcription of the other oncogenes studied , and without inducing cell division . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| C myc expression in lymphocytes of MRL / MP lpr mice activated by A 23187 and TPA . ^^^ Stimulation by concanavalin A ( Con A ) plus 12 O tetradecanoyl phorbol 13 acetate ( TPA ) or A 23187 plus TPA markedly increased c myc mRNA expression and cell proliferation , whereas stimulation by Con A alone failed to do so . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| TPA and A 23187 synergistically induced both IL 2R and c myc mRNA expression by the lpr Lyt 2 L3T4 T cells , as well as by normal T cells . ^^^ Cycloheximide treatment caused the TPA / A23187 stimulated lpr cells to express large amounts of c myc mRNA , but not IL 2R mRNA . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| It was reported that TPA treatment alters the expression of some protooncogenes , like c myc and c fos , involved in the induction of cell proliferation , and c fos and c fms ( CSF . 1 receptor ) , linked to monocytic differentiation . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| The extent and kinetics of c myc oncoprotein induction have been determined following phorbol ester , 12 O tetradecanoylphorbol 13 acetate ( TPA ) and interferon gamma ( IFN gamma ) exposure of both HL 60 and Daudi cells . ^^^ TPA produced a gradual reduction in the level of c myc protein and arrested the cells in G0 / G1 phase in HL 60 cells . ^^^ However , TPA failed to reduce c myc protein or to change cell cycle distribution in Daudi cells . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| We observed strong induction of c fos and to a lesser extent of c myc also by TPA , and by the calcium ionophore A 23187 , indicating an important role for kinase C in proto oncogene activation by growth factors . . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| The induction of differentiation in SH SY5Y human neuroblastoma cells with 12 O tetradecanoylphorbol 13 acetate ( TPA ) is accompanied by a rapid and a transient expression of c fos mRNA and a down regulation of c myc mRNA . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Taken together these results and previous work on the induction of the protooncogene c myc and c fos suggest that this B CLL clone represents GO cells that undergo differentiation without concomitant proliferation when exposed to TPA . . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Cells treated with 1 10 10 ( 7 ) mol / L and 1 10 10 ( 9 ) mol / L ara C did not evidence any of the changes in c myc , c myb , c fos , or c fes that are noted when dimethyl sulfoxide ( DMSO ) or 12 0 tetradecanoyl phorbol 13 acetate ( TPA , Sigma Chemical Corp , St Louis ) are used to induce the differentiation of HL 60 cells . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| In contrast , c myc mRNA expression is both in TPA and DMSO induced differentiation reduced to less then 5 % of its initial level within 4h . ^^^ Nuclear run on analysis revealed that the reduction of c myc mRNA expression during both TPA and DMSO induced differentiation could be ascribed to the abolition of transcription of the third exon , whereas no change in the transcription of the first exon could be observed . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| However , an early ( 0 . 5 8 . 0 h post induction ) transient reduction of c and N myc transcripts were observed in these cells upon induction to differentiation with the phorbol ester 12 O tetradecanoylphorbol 13 acetate ( TPA ) . ^^^ Treatment of these neuroblastoma cells with TPA for longer periods ( 1 8 days ) , which induces morphological and functional differentiation and growth arrest , was followed by decreased expression of both myc genes . ^^^ Thus , the TPA induced neuronal differentiation of SH SY5Y cells was compatible with high c fos and a substantial N myc mRNA expression . . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| The rat thyroid cell line ( FRTL 5 ) is dependent on thyrotropic hormone ( TSH ) for its growth . c fos and c myc oncogenes expression was measured in these cells after addition of their specific growth factor TSH and after treatment with either forskolin , an activator of adenylate cyclase or with a tumor promoter , TPA . ^^^ In contrast , in 5 ras transformed rat thyroid cells , which express very high levels of p 21 , treatment with either TSH , forskolin or TPA does not induce c fos gene expression , while c myc expression was constitutive . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Prolonged treatment of the cells with TPA , which causes a selective decrease in protein kinase C activity , partially inhibited the induction of c fos and c myc gene expression by bombesin , similar to what has been observed with PDGF . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Stimulation of B lymphocytes with TPA plus ionomycin resulted in increased magnitude and a shift in the kinetics of c fos and c myc expression compared with either agent used alone . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| In freshly isolated thymocytes c myc mRNA is expressed at low levels , while thymocytes activated with Concanavalin A ( Con A ) , the tumor promoter 12 O tetradecanoylphorbol 13 acetate ( TPA ) , Con A in combination with TPA or interleukin 2 ( IL 2 ) are induced to express higher levels of c myc mRNA . ^^^ Dexamethasone and Cyclosporin A ( CsA ) which inhibit early events of T cell activation and the expression of the interleukin 2 ( IL 2 ) and gamma interferon ( gamma IFN ) genes also markedly suppress the expression of c myc mRNA in Con A , and Con A + TPA activated thymocytes . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| A single treatment with TPA ( 1 microgram / ml ) increased the transcription of c fos and c myc proto oncogenes at least 20 fold in the mortal cell line HU 1752 . ^^^ When immortalized cell lines were treated with TPA a similar rapid and transient morphological response was observed , but the TPA treatment only increased the level of c fos mRNA , suggesting that the normal regulation of c myc transcription is altered in immortalized cells irrespective of their tumorigenic properties . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| The effect of a single treatment with the skin tumor promoter 12 O tetradecanoylphorbol 13 acetate ( TPA ) on the expression of the cellular proto oncogenes , c myc , c rasHa , c rasKi and c fos was examined in the non tumorigenic human bladder epithelial cell line HCV 29 . ^^^ The expression of c myc , c rasHa and c rasKi were not enhanced by the TPA treatment . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Treatment of U 937 cells with 12 O tetradecanoyl phorbol 13 acetate ( TPA ) which is associated with the induction of a monocytic differentiation program and growth arrest , revealed an initial up regulation of c myc , c max , and mxi 1 mRNAs after 1 6 h . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| The results of slot blot hybridizations showed that tumor promoter TPA induced c fos and c myc expressions in mouse fibroblast cell line BALB / 3T3 and rat liver , decreased the levels of Rb RNA in BALB / 3T3 cell line and of alpha 1 I 3 RNA in rat liver . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| In this cell line , a transient suppression of HPV transcripts was observed at 5 h , whereas in differentiation resistant , carcinoma derived lines , TPA had little effect on HPV oncogene expression . c myc transcripts were suppressed for the duration of exposure to TPA in only the differentiation competent cells ; c fos and c jun were transiently induced in all cell lines . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| The AP 1 consensus sequences ( TGAGTCA ) are the major 12 O tetradecanoylphorbol 113 acetate ( TPA ) responsive elements shared by several TPA inducible genes , such as c sis , c fos , c myc , collagenase , stromelysin , hMTIIA and SV 40 . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Induction of differentiation of HL 60 cells by exposure to dimethylsulfoxide or 12 o tetradecanoylphorbol 13 acetate ( TPA ) did not alter the level of H 4 acetylation within either the c myc or c fos genes or other coding regions , but did induce a transient increase in H 4 acetylation within centric heterochromatin . . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Treatment with tetradecanoyl phorbol acetate ( TPA ) , a potent activator of protein kinase C ( PKC ) , in combination with IL 7 induced a level of c myc expression greater than that elicited by either factor alone , suggesting that TPA and IL 7 utilize cooperative signaling pathways to increase c myc gene expression . . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| This study was undertaken to assess the effects of a single or two sequential topical applications of 12 O tetradecanoylphorbol 13 acetate ( TPA ) on the expression of c fos , c jun , junB , c myc , and ornithine decarboxylase ( ODC ) in promotion sensitive SSIN mice and the relatively promotion resistant C57BL / 6 strain . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Regulation of cell growth and the c myc proto oncogene expression by phorbol ester 12 0 tetradecanoyl phorbol 13 acetate ( TPA ) in the androgen independent human prostatic JCA 1 cells . ^^^ Following a 24h , 48h , and 72h treatment , with 16 nM TPA , c myc mRNA was suppressed by 91 % , 83 % , and 78 % , respectively , in good agreement with the extent of growth reduction observed . ^^^ At the low dose of TPA ( 0 . 16 nM ) , however , the c myc mRNA expression remained inhibited by 85 % even though cell growth was only reduced by 10 14 % . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| MIL suppressed c myc and enhanced NF kappa B p 50 transcription in the U 937 cell line , comparable to the well characterised differentiation inducing phorbolester 12 O tetradecanoylphorbol 13 acetate ( TPA ) . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Treatment with 10 nM TPA , which also induces keratinocyte differentiation , reduced c myc RNA levels to 70 % of control levels during the first 4 h , but thereafter c myc levels remained approximately constant for a further 20 h . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Curcumin inhibits TPA induced expression of c fos , c jun and c myc proto oncogenes messenger RNAs in mouse skin . ^^^ In the present studies , we investigated the effect of curcumin on the expression of c fos , c jun and c myc oncogenes in TPA treated mouse skin in CD 1 mice . ^^^ A 30 nmol dose of TPA increased the levels of mRNAs for c fos , c jun and c myc oncogenes by 2 3 fold compared with control . ^^^ A dose of 10 mumol of curcumin was found to inhibit 90 % TPA induced expression of c fos and c jun , and 60 % of c myc . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| We investigated the effects of Bryo and TPA on DNA synthesis , proliferation , viability and c myc protooncogene expression of the human carcinoma cell lines COLO 320 , MEL HO , and KB 3 1 . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| The expression of four genes necessary for DNA synthesis in response to tumor promoters was examined : two nuclear proto oncogenes ( c myc and c fos ) , a transcription factor ( c jun / AP 1 ) and a key enzyme involved in polyamine synthesis ( ornithine decarboxylase ) . c jun mRNA levels are not modulated during the action of cytoskeletal disrupting drugs on TPA mediated mitogenesis , whereas c myc and c fos mRNA levels are similarly enhanced . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| The phorbol ester , 12 O tetradeconoylphorbol 13 acetate ( TPA ) , can not support growth of these cells , is a more effective inducer than insulin of c fos , c myc , c jun , jun B , Krox 20 , Krox 24 , fra 1 and JE , and induces fra 1 , JE and c myc with different kinetics from those of insulin . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| C myc , c myb , c fos , c sis and c fms expression were determined by Northern analysis at various times following TPA treatment . ^^^ The results showed that TPA down modulated the constitutive expression of c myc , c myb , and c fms , mRNA to low but still detectable levels . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| In the second experiment , a single application of 10 nmol of TPA to mouse skin led to a marked increase in the transcripts ' level of ornithine decarboxylase ( ODC ) gene , protein kinase C ( PKC ) gene , and c myc oncogene at four hours after TPA administration . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| In our effort to explore genes implicated in neuritic extension and neuronal differentiation seen in response to cAMP and TPA , we have analyzed time dependent induction of a variety of proto oncogenes : c myc , H ras , c ras , and c fos . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Adrenocorticotropin ( ACTH ) treatment increased c myc mRNA accumulation dose and time dependently up to more than 5 fold ( on average ) , with the maximal effect at 2 h . ( Bu ) 2cAMP and 12 O tetradecanoyl phorbol 13 acetate ( TPA ) also induced c myc gene expression . ^^^ H 7 totally abolished ACTH , TPA and ( Bu ) 2cAMP induced c myc expression , while staurosporine inhibited the stimulatory effects of ACTH and TPA , and HA 1004 weakly inhibited the effects of ACTH and ( Bu ) 2cAMP . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Phorbol 12 myristate 13 acetate ( TPA ) further stimulated c fos and c myc expression , whereas treatment with 1 , 25 vitamin D 3 ( 10 ( 7 ) M ) it had no effect either in unstimulated or in TPA stimulated cells . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Surprisingly , all RU clones resisted the suppressing effect of 12 O tetradecanoylphorbol 13 acetate ( TPA ) on c myc oncogene expression and cell proliferation . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Similar to c myc , expression of the 4 kb transcript increased in Nb 2 11C cells given PRL for 3 h ( + / the phorbol ester TPA ) but not in cells given TPA alone . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Expression of the c myc gene was down regulated and c jun and c fms transcripts increased following exposure to 5 500 nM TPA . ^^^ In contrast , exposure to 0 . 5 nM TPA decreased c myc expression and increased c jun transcripts only transiently between 4 and 8 h while little if any effect was detectable on c fms mRNA expression and subsequent differentiation . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| In this context , Western blot analysis of the myc family revealed high levels of c myc protein accumulation in TUR cells even in the presence of TPA . ^^^ In contrast , incubation of U 937 cells with phorbol ester was associated with progressive down regulation of c myc protein . c myc can also form transcriptionally active heterodimeric complexes with the nuclear phosphoproteins p20 / p22 max : thus , TPA treatment resulted in down regulation of the p 20 form of max in TUR cells . ^^^ Another regulatory factor in the myc family , mad 1 , was expressed unaltered in U 937 and in TUR cells regardless of TPA stimulation . . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| In addition to estrogen , epidermal growth factor ( EGF ) and tumor promoter 12 O tetradecanoylphorbol 13 acetate ( TPA ) can also induce c myc expression with no effect on c fos or lactoferrin expression . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| The regulation of the c myc mRNA by TPA correlated inversely with activation of cell death being down regulated in LNCaP cells , and slightly increased in the androgen independent cell lines . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| PAI 1 inhibits the mitogenic effect of tPA to SMC and is dose dependent . tPA increases oncogene c myc mRNA level during SMC proliferation , and the level of c myc mRNA increases significantly in hyperlipidemia . ^^^ These findings indicate that tPA directly promotes human vascular SMC proliferation in vitro , and may contribute to intimal SMC proliferation after vascular injury by increasing the expression of oncogene c myc mRNA . . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Overexpression of c Myc inhibits p21WAF1 / CIP1 expression and induces S phase entry in 12 O tetradecanoylphorbol 13 acetate ( TPA ) sensitive human cancer cells . ^^^ Blagosklonny , et al . , Cancer Res . , 57 : 320 325 , 1997 ) that exposure of human SkBr 3 breast cancer and LNCaP prostate cancer cells to 12 O tetradecanoylphorbol 13 acetate ( TPA ) led to a growth arrest associated with the up regulation of the cyclin dependent kinase inhibitor p 21 ( WAF1 / cIP1 ) and the inhibition of c Myc expression . ^^^ We show here that exogenous c Myc inhibits p 21 expression in SkBr 3 and LNCaP cells induced to enter into S phase . p 27 expression was not increased from basal levels in TPA treated growth arrested cells . ^^^ A time course after infection of TPA arrested cells using a c Myc expressing adenovirus revealed that the inhibition of p 21 expression preceded entry into S phase . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Our results show that TPA + IFN gamma treatment led to an inhibition of 5 Myc and c Myc dependent transcription , and a specific reduction of 5 Myc : Max complexes and associated DNA binding activity , whereas the steady state level of the 5 Myc protein was only marginally affected . ^^^ Constitutive expression of 5 Myc blocks phorbol ester ( TPA ) induced differentiation of human U 937 monoblasts . ^^^ However , costimulation with interferon gamma ( IFN gamma ) and TPA restores terminal differentiation and G 1 cell cycle arrest despite continuous expression of 5 Myc . ^^^ The mechanism by which TPA + IFN gamma counteract 5 Myc activity has not been unravelled . ^^^ Phosphatase treatment of Myc : Max complexes lead to their dissociation , thus mimicking the effect of TPA + IFN gamma . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| These data suggest that dietary CLA modulates TPA induced tumor promotion through a mechanism involving PGE 2 production ; however , dietary CLA had a moderate effect on c myc mRNA levels and little effect on TPA induced hyperplasia and ornithine decarboxylase activity . . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| As judged by the reverse transcriptase polymerase chain reaction , resveratrol selectively inhibited TPA induced expression of c fos and transforming growth factor beta 1 ( TGF beta 1 ) , but did not affect other TPA induced gene products including COX 1 , COX 2 , c myc , c jun , and tumor necrosis factor alpha . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| TPA treatment also elevates the expression of cyclooxygenase 1 ( COX 1 ) , cyclooxygenase 2 ( COX 2 ) , c myc , c fos , c jun , transforming growth factor beta 1 ( TGF beta 1 ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| In addition , the expression of TPA induced c myc mRNA was significantly inhibited by the same InsP 6 treatments in HEL 30 cells and CD 1 mouse skin ( P < 0 . 01 ) . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| We have studied the regulation and role of c Myc and Max in the differentiation pathways induced in K 562 cells by 12 O tetradecanoyl phorbol 13 acetate ( TPA ) and staurosporine , an activator and inhibitor , respectively , of protein kinase C ( PKC ) . ^^^ In contrast , TPA induced a differentiated phenotype that more closely resembled that of the monocyte macrophage lineage . c myc expression was down regulated in K 562 differentiated by both TPA and staurosporine , whereas max expression did not change in either case . ^^^ Although PKC enzymatic activity was low in cells terminally differentiated with TPA and staurosporine , inhibition of PKC activity by itself did not induce c myc down regulation . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Topical application of 5 nmol TPA to CD 1 mice once daily for 5 days caused epidermal hyperplasia , and increases in the levels of c Fos , c Jun and c Myc in the suprabasal layer of epidermis and the muscle layer of dermis . ^^^ Immunocytolochemical examination showed that pretreatment of 1 mumol crocetin repressed the TPA induced epidermal hyperplasia and the expressions of c Jun , c Fos and c Myc to the extent of 47 , 44 and 45 % respectively . ^^^ These data indicate that crocetin suppresses the TPA induced skin carcinogenesis maybe via its antioxidant property which , in turn , leads to a reduction in the TPA induced expressions of c Jun , c Fos and c Myc in mouse epidermis . . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Downregulation of c myc mRNA and upregulation of c fos and egr 1 mRNA and protein , which normally occur during TPA induced differentiation , were not affected by inclusion of the protease inhibitors . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| The levels of c Myc and Mad 1 mRNAs and proteins increased within 3 h of anti mu stimulation , and the levels were further enhanced by TPA . ^^^ Furthermore , the expressions of both c Myc and Mad 1 were reduced by forskolin , which also inhibited the anti mu + TPA driven growth and differentiation of the B lymphocytes . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| TPA induced differentiation of K 562 cells causes rapid down regulation of c myc expression , due in part to an mRNA decay rate that is 4 fold faster compared with dividing cells . ^^^ A cell free mRNA decay system reconstitutes TPA induced destabilization of c myc mRNA , but it requires at least two components for reconstitution . ^^^ Although in dividing cells c myc mRNA decays via a sequential pathway involving removal of the poly ( A ) tract followed by degradation of the mRNA body , TPA activates a deadenylation independent pathway . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Topical application of curcumin was reported to inhibit TPA induced c fos , c jun and c myc gene expression in mouse skin . ^^^ CONCLUSIONS : Whereas earlier work demonstrated that topical application of curcumin to mouse skin inhibited TPA induced expression of c fos , c jun and c myc oncogenes , our results are the first to show that orally consumed curcumin significantly inhibited DMBA and TPA induced ras and fos gene expression in mouse skin . . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Other genes strongly upregulated in the highly invasive cell clone included jagged 2 , STAT 1 , tPA and c myc , whereas MDA 6 ( p 21 ) , caspase 2 and semaphorin were found to be downregulated . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Constitutive expression of c myc in as CD11b transfectants was higher than in control cells and failed to be repressed by TPA treatment . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Her2 / neu potentiated tumor necrosis factor alpha ( TNFalpha ) inducible NF kappaB activation whereas c Myc potentiated 12 o tetracecanyolphorbol 13 acetate ( TPA ) induced NF kappaB activation . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Furthermore , sericin treatment significantly suppressed the elevation in 4 HNE level and elevated expressions of c fos , c myc and cyclooxygenase 2 ( COX 2 ) in normal epidermis induced by double application of TPA . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Transgenic mice expressing T antigen and c myc under the control of the elastase promoter ( Ela 1 TAg and Ela 1 myc , respectively ) were used . tPA was undetectable in normal pancreas , acinar dysplasia , ductal complexes , and in all acinar tumors . ^^^ |
|
| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| In this study , we found that TPA up regulated phosphorylation of p 38 , a mitogen activated protein kinase , and activated c myc mRNA in EBV positive epithelial GT 38 cells . ^^^ Protein kinase C inhibitors , 1 ( 5 isoquinolinesulphonyl ) 2 , 5 dimethylpiperazine ( H 7 ) and staurosporine , inhibited the induction of p 38 phosphorylation and the activation of c Myc by TPA . ^^^ Pretreatment of GT 38 cells with the nitric oxide ( NO ) donor S nitroso N acetylpenicillamine inhibited p 38 phosphorylation and c Myc activation by TPA , suggesting that NO may inhibit EBV reactivation via both p 38 and c Myc . ^^^ Our present study demonstrates for the first time that either p 38 or c myc siRNA can efficiently inhibit TPA induced EBV reactivation in GT 38 cells , indicating that p 38 and / or c myc associated signaling pathways may play critical roles in the disruption of EBV latency by TPA . . ^^^ |
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| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Arsenic / TPA treatment resulted in increased expression of alpha fetoprotein , k ras , c myc , estrogen receptor alpha , cyclin D 1 , cdk2na , plasminogen activator inhibitor 1 , cytokeratin 8 , cytokeratin 18 , glutathione S transferases and insulin like growth factor binding proteins in liver and liver tumors from both male and female mice . ^^^ |
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| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| Different types of tumors developed in transgenic mice following the introduction of the entire coding region of ras , myc or SV 40 large T gene ( T ) linked to the same regulatory unit , consisting of a human immunoglobulin gene enhancer ( Ig ) and SV 40 early gene promoter ( Tp ) with a 21 bp repeat . ^^^ |
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| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| By Northern analysis of mRNA after serum addition , TPI 1 caused a fourfold decrease in the transient elevation of fos and myc proto oncogene as well as a decrease in the levels of both muscle and nonmuscle actin mRNA induced early after serum addition . ^^^ |
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| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| TP treatment of mice with LNCaP 104 R 2 tumors reduced tumor androgen receptor and c myc mRNA levels but increased prostate specific antigen in serum and prostate specific antigen mRNA in tumors . ^^^ |
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| Interacting proteins: P42166 and P01106 |
Pubmed |
SVM Score :0.0 |
| In the early period of leukaemia growth ( NTP ) only 14 . 33 % of c myc positive cells were found , as opposed to the terminal phase ( TP ) of leukaemia when 89 . 7 % of c myc positive cells were detected . ^^^ |
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