| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| Northern blot analysis indicated that the expression of genes induced during T cell activation , such as those encoding interleukin 2 ( IL 2 ) , c myc , IL 2 receptor , IL 6 , c myb , and cdc 2 , was not significantly suppressed in MV infected cells , suggesting that MV does not interfere with the T cell activation process . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| Northern Blot kinetic analysis of c fos , c myc , cdc 2 , IL 2R , c myb , ODC , TK and H 3 , from 10 minutes to 120 hours , was performed in MAb 01 . 65 treated cultures . ^^^ We found that the expression of four genes ( c myc , IL 2R , cdc 2 and TK ) was depressed 24 hours after PHA stimulation . . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| At the gene expression level , sodium butyrate does not affect the inducibility of early cell cycle related genes ( c myc , c jun ) while blocking the induction of cdc 2 mRNA , a late G 1 marker . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| Early events in T cell activation , as assessed by steady state mRNA levels of c myc , IL 2 , c myb , histone , and cdc 2 kinase , as well as surface IL 2 receptor expression , were unaffected . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| Antisense oligodeoxynucleotides could reduce the increase in cdc 2 expression and inhibited DNA synthesis , but had no effect on several early and mid G 1 events , including blastogenesis and expression of interleukin 2 receptors , transferrin receptors , c myb , and c myc . ^^^ Induction of cdc 2 required prior induction of c myb and c myc . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| The transcription factor E2F plays a pivotal role in the coordinated transactivation of cell cycle regulatory genes such as c myc , cdc 2 , and the gene encoding proliferating cell nuclear antigen ( PCNA ) that are involved in lesion formation after vascular injury . ^^^ Transfection with E2F decoy inhibited expression of c myc , cdc 2 , and the PCNA gene as well as vascular smooth muscle cell proliferation both in vitro and in the in vivo model of rat carotid injury . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| Cells en route to apoptosis are characterized by the upregulation of c fos , c myc , c jun , cdc 2 , and RB phosphorylation , resembling events of early cell cycle traverse . ^^^ Investigating the regulation of early cell cycle genes during this process , we found that c myc , c jun , c fos , and cdc 2 protein presence is induced after serum deprivation , when the phosphorylated form of the RB protein also appears . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| Extinction of the cdc 2 gene expression is also observed in BP A 31 cells overexpressing the human c myc oncogene . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| Northern analysis of the total RNA extracted from these cells showed the accumulation of IL 2R alpha chain specific mRNA but neither c myc nor cdc 2 mRNA was expressed . ^^^ The addition of 100 nM rIL 2 to T cells activated by phorbol dibutyrate was able to induce a proliferative response , and under these conditions tyrosine phosphorylation of the 97 kDa protein , the transient increase in p56lck kinase activity , and specific mRNA for IL 2R alpha chain , c myc , and cdc 2 were detected . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| MSSP 1 binds to a DNA replication origin / transcriptional enhancer of the human c myc gene and has turned out to be identical with Scr 2 , a human protein which complements the defect of cdc 2 kinase in S . pombe [ Kataoka and Nojima ( 1994 ) Nucleic Acid Res . , 22 , 2687 2693 ] . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| The fluctuations in the levels of the proliferation cell nuclear antigen ( PCNA ) , cyclin A , CDC 2 , c myc , and p 53 proteins were monitored in synchronized cultures and in cells undergoing apoptosis by immunofluorescence staining , flow cytometry , and Western immunoblotting . ^^^ Most importantly , however , the level of staining for c myc , p 53 , cyclin A , CDC 2 , and PCNA increased 50 % 150 % during apoptosis compared to the levels observed in cells at G1 / S . ^^^ AGF cells undergoing apoptosis were immunostained for c myc , p 53 , PCNA , cyclin A , and CDC 2 and were viewed by confocal microscopy . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| BON cells also exhibited increased expression of c myc and cdc 2 kinase mRNA and protein ; TGF beta 1 , p 53 and retinoblastoma ( RB ) mRNA and protein levels were decreased . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| We report here that StSp alone and combined with mAb 01 . 65 affects also the expression of c myc and cdc 2 and the membrane expression of two proliferation markers : IL 2R ( CD 25 ) and TfR ( CD 71 ) . . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| We also found that apoptotic mouse 3T3 fibroblasts express c fos , c myc , c jun , and cdc 2 , as well as the upregulation of RB phosphorylation and BrdU incorporation , just before final DNA fragmentation and death . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| Although both p 42 mitogen activated protein kinase and cdc 2 kinase specifically phosphorylate Ser 62 in vitro and cellular phosphorylation of Thr 58 / Ser 62 is stimulated by mitogens , other in vivo experiments do not support a role for these kinases in the phosphorylation of Myc proteins . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| Since c Myc has been shown to function as a transcriptional activator , we have examined the ability of c Myc and activated Ras ( H RasV 12 ) to cooperatively induce the promoter activity of cdc 2 , a gene which is critical for cell cycle progression . ^^^ Microinjection of expression constructs encoding H RasV 12 and c Myc along with a cdc 2 promoter luciferase reporter plasmid into quiescent cells led to an increase in cdc 2 promoter activity approximately 30 h after injection , a period which coincides with the S to G2 / M transition in these cells . ^^^ Expression of H RasV 12 alone weakly activated the cdc 2 promoter , while expression of c Myc alone had no effect . ^^^ Mutants of c Myc lacking either the leucine zipper dimerization domain or the phosphoacceptor site Ser 62 could not cooperate with H RasV 12 to induce the cdc 2 promoter . ^^^ Deletion analysis identified a distinct region of the cdc 2 promoter which was required for c Myc responsiveness . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| In the refractory cells , IL 3 was able to stimulate protein tyrosine phosphorylation and c myc mRNA accumulation , but not rapid Erk 2 activation or cdc 2 mRNA accumulation . . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| The presence of antisense c myc oligonucleotide inhibited the expression of cdc 2 and cdk 2 without affecting the expression of IL 2R alpha and blocked the activated T cells in the G 1 phase . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| The `` low risk ' ' human papillomavirus types ( HPV 6 or 11 ) were determined by in situ hybridization and PCR amplification followed by direct sequencing using consensus primers from the highly conserved L 1 region in six different condylomas . mRNA levels of certain cytokines ( e . g . , TGF beta 1 , IFN beta ) , tumor suppressor genes ( RB , p 53 ) , c myc , epidermal growth factor receptor , and cdc 2 kinase were measured by RT / PCR . ^^^ Western blot experiments demonstrated a higher proportion of the hyperphosphorylated form of RB protein and a higher level of cdc 2 kinase and c myc , but low p 53 and TGF beta 1 levels in condylomas . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| Viral oncoproteins , such as adenovirus E1a and SV 40 large T antigen , overcome such repression by sequestering pRb and p 107 and in so doing are likely to activate genes regulated by DRTF1 / E2F , such as cdc 2 , c myc and DHFR . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| In addition , ligation of the IL 2R leads to rapid increases in myc expression and more delayed increases in the expression of the cdc 2 and cdk 2 kinases and the cyclins through a tyrosine phosphorylation independent pathway . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| We also examined the effect of TGF beta 1 on the expression of proliferation associated `` immediate early ' ' genes ( zif 268 , jun B , c myc ) during the early G 1 phase and the cdc 2 gene during the transition from the G 1 phase to the S phase of the cell cycle . ^^^ The genes activated during G 1 can be divided into TGF beta 1 insensitive ( zif 268 , jun B , and c myc ) and TGF beta 1 sensitive ( the cdc 2 gene ) . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| Moreover , TGF beta 1 induced the mRNA expression of a complete set of proliferation related genes , such as c fos ( 30 min ) , c myc and beta actin ( 2 h ) , and H ras , cdc 2 kinase , and glucose 6 phosphate dehydrogenase ( G6PD ) at 4 and 8 h , as compared to quiescent cells . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| In conclusion , PKC alpha joins the group of proteins such as c myc , proliferating cell nuclear antigen and cdc 2 kinase which may be therapeutical targets , for antisense oligodeoxynucleotides , in order to prevent SMC hyperplasia . . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| Manifestations of these variations were examined in terms of the expression at the mRNA level ( as measured by reverse transcriptase polymerase chain reaction ) of three regulatory genes ( cdc 2 , c myc , and p 53 ) and five that encode structural proteins ( keratins K 5 , K 10 and K 13 , involucrin , and filaggrin ) , in three growth medium formulations . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| To determine whether these alterations were the consequence of E6 / E7 protein expression or whether they resulted from the process of cellular immortalization , we multiply infected primary genital keratinocytes with a retrovirus expressing the HPV 18 E6 / E7 genes and examined the cells for acute , pre immortalization changes in several critical cell growth regulatory proteins including cyclin A , cyclin B , cdc 2 , p 53 and c myc . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| IFN gamma caused a rapid ( within 12 24 h ) down regulation of cyclin A , c myc , and cdc 2 proteins , as well as a disappearance of hyperphosphorylated forms of the retinoblastoma family proteins , Rb and p 130 . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| The response of the cells to TGF beta 1 was monitored in terms of the expression of regulatory genes associated with proliferation and differentiation ( cdc 2 , c myc , p 53 ) and of genes for structural proteins expressed at varying stages of maturation ( keratins K 5 and K 10 , involucrin , flaggrin ) . ^^^ For both tissues , the results obtained with both agents were very similar for those genes expressed in the basal cells ( cdc 2 , c myc , p 53 , K 5 ) , regardless of their function , but diverged for the other genes , which are expressed in the suprabasal cells . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| The most promising experimental results have been obtained with oligonucleotides targeted against genes involved in cell proliferation , such as c myc , c myb , Kras , and cdc 2 . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| The membranes were stained with amido black , scanned and tested separately with the nine monoclonal antibodies p 53 , c myc , PCNA , MEK 1 , pan ras , Cip 1 , Cdc 2 , Kip 1 , and TCTP . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| The procedure has been used to identify new ts alleles of cdc 2 and swi7 / poll , a ts mutant rescued by actin , and to identify a ts allele of cdc 11 which can be rescued by combined mammalian Myc and Max expression . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| Inactivation of a c Myb / estrogen receptor fusion protein in transformed primary cells leads to granulocyte / macrophage differentiation and down regulation of c kit but not c myc or cdc 2 . ^^^ The expression of the c myc , c kit , cdc 2 and bcl 2 genes , which are putatively regulated by Myb , was investigated in ERMYB cells grown in the presence or absence of beta estradiol . ^^^ Neither c myc nor cdc 2 expression was down regulated after removal of beta estradiol demonstrating that differentiation is not a consequence of decreased transactivation of these genes by ERMYB . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| The existence of increased E2F activity during hepatocarcinogenesis was further indicated by the transcriptional induction of putative E2F target genes involved in cell cycle progression , such as endogenous c myc , cyclin A , Cdc 2 , and E2F itself . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Analyzing mRNA levels of various epithelial genes ( c myc , cdc 2 kinase , E2F , PCNA , p 53 , and RB , cytokeratins 5 and 10 ) we concluded that MCCs express markers of epithelial hyperproliferation together with markers of neuroendocrine differentiation ( NSE ) . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| Western blot analysis of six samples showed a high level of expression of c myc and cdc 2 kinase and a low level of p 53 protein in NPCAs that contained both HPV and EBV ( n = 3 ) . ^^^ Increased expression of c myc and cdc 2 kinase was seen in the cases that contained EBV only , but to a lesser extent ( n = 2 ) . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| To test this hypothesis , a recently developed quantitative reverse transcriptase polymerase chain reaction method was used to assess simultaneously expression of 15 genes mechanistically associated with cell cycle control ( c myc , E2F 1 , p 21 , rb , PCNA , cyclin D 2 , cyclin D 3 , cyclin E , cdc 2 , CDK 2 , CDK 4 , mad , max p 21 , max p 22 , and p 53 ) in normal cell cultures from five individuals and in nine different malignant BEC lines . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| Transient transfection assays have implicated pRb in the transcription of several genes , including c fos , the interleukin 6 gene , c myc , cdc 2 , c neu , and the transforming growth factor beta 2 gene . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| The E2F decoy , transferred by HVJ CL , inhibited simultaneously the expression of the mRNAs of several cell cycle related genes such as c myc , cdc 2 , proliferative cell nuclear antigen , and dehydrofolate reductase . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| Both the initiation and progression of mitotic events were confirmed by the expression of c myc and cdc 2 in the presence of NT 3 , PDGF AA or NT 3 plus PDGF AA . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| Whereas no substantial changes in c myc mRNA levels and p 21 , PCNA , cdc 2 , and CDK 2 protein levels were noted in PMEA treated K 562 cells , there was a marked accumulation of cyclin A and , most strikingly , cyclins E and B 1 . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| Moreover , PS oligodeoxynucleotides targeted against the genes c myb , c myc , cdc 2 kinase , cdk 2 kinase , and proliferating cell nuclear antigen ( PCNA ) when delivered adventitially or intraluminally inhibit in vivo neointimal formation after balloon injury in both the rat carotid and porcine coronary artery models . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| Other E2F regulatory genes , including c myc , cyclin A , and cdc 2 , were also down regulated in mesangial cells cultured on polymerized collagen matrix . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| To explore whether GGT affects the transcription of cell cycle regulating genes , we evaluated mRNA expression of p 53 , p21Waf1 PCNA , Cyclin D 1 , Cdc 2 , Histone H 3 , c Myc , and c Fos using a quantitative RT PCR analysis . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| These include alterations in DNA ploidy , cellular proliferation markers ( PCNA , Ki 67 , Mcm 2 , MIB 1 , MIA , and CSE1L / CAS protein ) , nuclear morphology , the p 53 gene and its related molecule MD M 2 , other cell cycle regulators ( cyclin A , cyclin D , cyclin E , cdc 2 , p 27 , p 73 ) , oncogenes and their receptors ( such as ras , c myc , c fms , HGF , c met , and erb B receptor family members ) , apoptosis related factors ( Fas and FasL ) , as well as telomerase activity . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| Leukemias with a 5 / del ( 5q ) have a higher expression of genes involved in cell cycle control ( CCNA 2 , CCNE 2 , CDC 2 ) , checkpoints ( BUB 1 ) , or growth ( MYC ) , and loss of expression of the gene encoding IFN consensus sequence binding protein ( ICSBP ) . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| Leukemias with a 5 / del ( 5q ) have a higher expression of genes involved in cell cycle control ( CCNA 2 , CCNE 2 , CDC 2 ) , checkpoints ( BUB 1 ) , or growth ( MYC ) , and loss of expression of the gene encoding interferon consensus sequence binding protein ( ICSBP ) . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| To elucidate that the cell cycle inhibitory effect of GJS stems from its capability of transcriptional regulation of the cell cycle controlling genes , we investigated mRNA expression of p 53 , Waf 1 , PCNA , Cyclin D 1 , Cdc 2 , Histone H 3 , c Myc , and c Fos . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| The expression of c myc , c fos , cdc 2 , p 21 , and G3PDH mRNAs in transfectants was not significantly changed . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| Our present study shows that c Myc was significantly induced and that it inhibited p 21 and induced proteins such as cyclin dependent kinases , cyclin D , and cyclin E , which are involved in the cell cycle process , along with phosphorylation of the retinoblastoma protein and Cdc 2 ( cell division cycle 2 ) . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| The gene order of CMII proviral DNA is 5 ' delta gag 5 myc delta pol delta env 3 ' . ^^^ |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28340 and P01106 |
Pubmed |
SVM Score :0.0 |
| NA |
|