| Interacting proteins: P01106 and P20226 |
Pubmed |
SVM Score :0.8191721 |
| These results indicate that intracellularly , c Myc interacts with TBP , suggesting a mechanism of interaction of this oncoprotein with the basal transcription machinery . . 0.8191721^^^ |
|
| Interacting proteins: P01106 and P20226 |
Pubmed |
SVM Score :0.59645414 |
| TBP probably interacts with Myc , but only slowly . 0.59645414^^^ |
|
| Interacting proteins: P01106 and P20226 |
Pubmed |
SVM Score :0.0 |
| Using a protein binding assay , we show that the amino terminal 204 amino acids of the c Myc protein interact directly with a key component of the basal transcription factor TFIID , the TATA box binding protein ( TBP ) . ^^^ Essentially the same region of the c Myc protein also binds the product of the retinoblastoma gene , the RB protein . c Myc protein coimmunoprecipitates with TBP in lysates of mammalian cells , demonstrating that the proteins are also complexed in vivo . ^^^ A short peptide that spans the RB binding site of the E 7 protein of human papilloma virus type 16 interferes with the binding of c Myc to TBP . ^^^ The same peptide also blocks binding of adenovirus E1A protein to TBP , suggesting that c Myc and E1A bind to RB and TBP through overlapping epitopes . ^^^ Our data suggest that one of the functions of RB and RB like proteins is to prevent interaction of viral and cellular oncoproteins , such as c Myc and E1A , with TBP . . ^^^ |
|
| Interacting proteins: P01106 and P20226 |
Pubmed |
SVM Score :0.0 |
| The c myc promoter binding protein ( MBP 1 ) and TBP bind simultaneously in the minor groove of the c myc P 2 promoter . ^^^ MBP 1 binds to a sequence which overlaps the binding site for the general transcription factor TBP , within the c myc P 2 promoter region . ^^^ Since TBP binds in the minor groove , MBP 1 might inhibit c myc transcription by preventing the formation of a functional preinitiation complex . ^^^ We conclude that both MBP 1 and TBP can bind simultaneously in the minor groove of the TATA motif on the c myc P 2 promoter . ^^^ |
|
| Interacting proteins: P01106 and P20226 |
Pubmed |
SVM Score :0.0 |
| Conversely , a PNA complementary to a unique sequence of the c myc gene did not inhibit transcription of the AR or TBP genes but did inhibit c myc transcription . ^^^ Comparisons of PNA effects on sense and antisense transcription of the AR , TBP , and c myc genes confirm that progression of the RNA polymerase complex beyond the site of PNA . ^^^ |
|
| Interacting proteins: P01106 and P20226 |
Pubmed |
SVM Score :0.0 |
| In a protein interaction assay , c myc 1 143 bound selectively to two basal transcription factors , the TATA binding protein ( TBP ) and the RAP 74 subunit of TFIIF . ^^^ Taken together , these results identify TBP as an important target for the c myc transactivation domain , during transcriptional initiation . ^^^ Strikingly , addition of TBP caused changes in the CD spectra consistent with induction of protein conformation in c myc 1 143 during interaction with the target factor . ^^^ |
|
| Interacting proteins: P01106 and P20226 |
Pubmed |
SVM Score :0.0 |
| It is the regulatory subunit of the DNA dependent protein kinase that phosphorylates many proteins , including SV 40 large T antigen , p 53 , RNA polymerase 2 , RP A , topoisomerases , hsp 90 , and many transcription factors such as c Jun , c Fos , oct 1 , sp 1 , c Myc , TFIID , and many more . ^^^ |
|
| Interacting proteins: P01106 and P20226 |
Pubmed |
SVM Score :0.0 |
| It was found that the TATA binding protein ( TBP ) , c MYC : and survivin genes are not hypermutated . ^^^ |
|
| Interacting proteins: P01106 and P20226 |
Pubmed |
SVM Score :0.0 |
| Through its transactivation domain ( TAD ) , c Myc protein interacts with several transcription factors , including TATA binding protein ( TBP ) . ^^^ We present data that suggest that in contrast to some other transcriptional activators , an extended length of the c Myc TAD is required for its binding to TBP . ^^^ |
|
| Interacting proteins: P01106 and P20226 |
Pubmed |
SVM Score :0.0 |
| We describe an RPM 1 interacting protein that is an ortholog of TIP49a , previously shown to interact with the TATA binding protein ( TBP ) complex and to modulate c myc and beta catenin mediated signaling in animals . ^^^ |
|
| Interacting proteins: P01106 and P20226 |
Pubmed |
SVM Score :0.0 |
| For the repression , the N terminal transactivation domain of p73alpha is also indispensable , arguing for the importance of the activity of p73alpha in the mechanism . p73alpha binds the C terminal HAP domain of NF YC previously found to be the interaction site with c Myc and TBP . ^^^ |
|
| Interacting proteins: P01106 and P20226 |
Pubmed |
SVM Score :0.0 |
| Further , either a phosphocellulose fraction of HeLa whole cell extract ( WCE ) enriched for transcription factor TF IIIB , or recombinant TATA box binding protein could restore the inhibited c myc pol 3 transcription under conditions that activate PKC . ^^^ |
|
| Interacting proteins: P01106 and P20226 |
Pubmed |
SVM Score :0.0 |
| Several transcription factor binding sequences like ATF / CREB , E2A PBX 1 , EBP , PEA 3 , ETS 2 , Sp 1 , c Myc , TBP , GATA 1 and Oct 3 were conserved in the promoter sequence . ^^^ |
|
| Interacting proteins: P01106 and P20226 |
Pubmed |
SVM Score :0.0 |
| A variety of functions of C MYC , the product of c myc , are attributed to protein protein interactions with various cellular factors including Max , YY 1 , p 107 , Bin 1 and TBP . ^^^ Max and YY 1 bind to the C terminal region of C MYC , while p 107 , Bin 1 and TBP bind to the N terminal region covering myc boxes . ^^^ |
|
| Interacting proteins: P01106 and P20226 |
Pubmed |
SVM Score :0.0 |
| Both enolase alpha and MBP 1 can bind to the P 2 element in the c myc promoter and compete with TATA box binding protein ( TBP ) to suppress transcription of c myc . ^^^ |
|
| Interacting proteins: P01106 and P20226 |
Pubmed |
SVM Score :0.0 |
| Thus , the entire N and C terminal regions of c Myc TAD act in concert to achieve high specificity and affinity to two structurally and functionally orthogonal target proteins , TBP and MM 1 , possibly through a mechanism involving molten globule formation . ^^^ |
|
| Interacting proteins: P01106 and P20226 |
Pubmed |
SVM Score :0.0 |
| They interact with many different proteins including TATA box binding protein ( TBP ) , beta catenin and c Myc and thus , potentially modulate different pathways . ^^^ |
|
| Interacting proteins: P01106 and P20226 |
Pubmed |
SVM Score :0.0 |
| CONCLUSION : Our observations not only demonstrate that TBP is limiting for Myc transactivation in transient transfection experiments , but they also suggest that the inclusion of TBP in Myc transactivation assays may further improve the characterization of c Myc target genes . . ^^^ |
|
| Interacting proteins: P01106 and P20226 |
Pubmed |
SVM Score :0.0 |
| We have selected three types of factors : ( 1 ) oncogene products such as MYC , c MYB and Maf ; ( 2 ) transcriptional activators regulated during the cell cycle as E2F and SRF ; and ( 3 ) general transcription factors such as TATA binding protein ( TBP ) , Sp 1 and NF Y . ^^^ |
|
| Interacting proteins: P01106 and P20226 |
Pubmed |
SVM Score :0.0 |
| A number of interaction partners of Myc have been identified , such as Max , p 107 , TBP , YY 1 , Miz 1 , AP 2 and Nmi . ^^^ |
|
| Interacting proteins: P01106 and P20226 |
Pubmed |
SVM Score :0.0 |
| Changes in the mRNA expression levels of seven genes , Tbp , Eef1a , Fos , Junb , Myc , Vegf , and Glut 2 , were determined by real time reverse transcription polymerase chain reaction . ^^^ Incubation at 25 degrees C for up to 4 h only slightly altered ( by a factor of less than 2 ) levels of mRNA for Tbp , Eef1a , Junb , Myc , Vegf , and Glut 2 . ^^^ |
|
| Interacting proteins: P01106 and P20226 |
Pubmed |
SVM Score :0.0 |
| We investigated the structural and dynamical properties of B Myc , free or associated with the transactivation inhibitor , MM 1 , and the activator , TBP , using NMR spectroscopy . ^^^ The local regions of B Myc involved in binding differ for MM 1 and TBP , and regions not identified by mutagenesis are found to be involved in MM 1 binding . ^^^ |
|
| Interacting proteins: P01106 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01106 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01106 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01106 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01106 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01106 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01106 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01106 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01106 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01106 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01106 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|