| Interacting proteins: P01040 and P09668 |
Pubmed |
SVM Score :0.70842225 |
| These results indicate that cystatin alpha was taken up by kidney lysosomes , formed a complex with cathepsin H and initially decreased the activity of cathepsin H , but that later the level of cystatin alpha in the kidney rapidly decreased . . 0.70842225^^^ |
|
| Interacting proteins: P01040 and P09668 |
Pubmed |
SVM Score :0.0 |
| Although the component protein , P cystatin alpha itself , inhibited cathepsin H strongly , these conjugated proteins inhibited specifically the cathepsin L family . ^^^ |
|
| Interacting proteins: P01040 and P09668 |
Pubmed |
SVM Score :0.0 |
| The opposite was true for stefin A ( p = 0 . 0002 ; p = 0 . 002 ) and stefin B ( p = 0 . 009 ; p = 0 . 003 ) , and in disease free survival also for cathepsin H ( p = 0 . 055 ) . ^^^ |
|
| Interacting proteins: P01040 and P09668 |
Pubmed |
SVM Score :0.0 |
| In comparison to normal sera , cathepsin H concentrations were elevated in all asthmatic patients , concentrations of both stefins were decreased in steroid independent asthmatics , and stefin A concentrations were increased in steroid dependent asthmatics before therapy . ^^^ |
|
| Interacting proteins: P01040 and P09668 |
Pubmed |
SVM Score :0.0 |
| Crystal structure of Stefin A in complex with cathepsin H : N terminal residues of inhibitors can adapt to the active sites of endo and exopeptidases . ^^^ The crystal structure of human stefin A bound to an aminopeptidase , porcine cathepsin H , has been determined in monoclinic and orthorhombic crystal forms at 2 . 8A and 2 . 4A resolutions , respectively . ^^^ On binding , the N terminal residues of stefin A adopt the form of a hook , which pushes away cathepsin H mini chain residues and distorts the structure of the short four residue insertion ( Lys155A Asp155D ) unique to cathepsin H . ^^^ Furthermore , comparison with the stefin B papain complex showed that molecules of stefin A bind about 0 . 8A deeper into the active site cleft of cathepsin H than stefin B into papain . ^^^ The approach of stefin A to cathepsin H induces structural changes along the interaction surface of both molecules , whereas no such changes were observed in the stefin B papain complex . ^^^ |
|
| Interacting proteins: P01040 and P09668 |
Pubmed |
SVM Score :0.0 |
| Recombinant human cathepsin H was inhibited by chicken cystatin , stefin A , and stefin B with the K ( 1 ) values in the range of 0 . 05 0 . 1 nM , which is slightly tighter than the inhibition of purified cathepsin H by the same inhibitors . ^^^ |
|
| Interacting proteins: P01040 and P09668 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P09668 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P09668 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P09668 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P09668 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P09668 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P09668 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P09668 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P09668 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P09668 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P09668 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P09668 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09668 and P01040 |
Pubmed |
SVM Score :0.70842225 |
| These results indicate that cystatin alpha was taken up by kidney lysosomes , formed a complex with cathepsin H and initially decreased the activity of cathepsin H , but that later the level of cystatin alpha in the kidney rapidly decreased . . 0.70842225^^^ |
|
| Interacting proteins: P09668 and P01040 |
Pubmed |
SVM Score :0.0 |
| Although the component protein , P cystatin alpha itself , inhibited cathepsin H strongly , these conjugated proteins inhibited specifically the cathepsin L family . ^^^ |
|
| Interacting proteins: P09668 and P01040 |
Pubmed |
SVM Score :0.0 |
| The opposite was true for stefin A ( p = 0 . 0002 ; p = 0 . 002 ) and stefin B ( p = 0 . 009 ; p = 0 . 003 ) , and in disease free survival also for cathepsin H ( p = 0 . 055 ) . ^^^ |
|
| Interacting proteins: P09668 and P01040 |
Pubmed |
SVM Score :0.0 |
| In comparison to normal sera , cathepsin H concentrations were elevated in all asthmatic patients , concentrations of both stefins were decreased in steroid independent asthmatics , and stefin A concentrations were increased in steroid dependent asthmatics before therapy . ^^^ |
|
| Interacting proteins: P09668 and P01040 |
Pubmed |
SVM Score :0.0 |
| Crystal structure of Stefin A in complex with cathepsin H : N terminal residues of inhibitors can adapt to the active sites of endo and exopeptidases . ^^^ The crystal structure of human stefin A bound to an aminopeptidase , porcine cathepsin H , has been determined in monoclinic and orthorhombic crystal forms at 2 . 8A and 2 . 4A resolutions , respectively . ^^^ On binding , the N terminal residues of stefin A adopt the form of a hook , which pushes away cathepsin H mini chain residues and distorts the structure of the short four residue insertion ( Lys155A Asp155D ) unique to cathepsin H . ^^^ Furthermore , comparison with the stefin B papain complex showed that molecules of stefin A bind about 0 . 8A deeper into the active site cleft of cathepsin H than stefin B into papain . ^^^ The approach of stefin A to cathepsin H induces structural changes along the interaction surface of both molecules , whereas no such changes were observed in the stefin B papain complex . ^^^ |
|
| Interacting proteins: P09668 and P01040 |
Pubmed |
SVM Score :0.0 |
| Recombinant human cathepsin H was inhibited by chicken cystatin , stefin A , and stefin B with the K ( 1 ) values in the range of 0 . 05 0 . 1 nM , which is slightly tighter than the inhibition of purified cathepsin H by the same inhibitors . ^^^ |
|
| Interacting proteins: P09668 and P01040 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09668 and P01040 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09668 and P01040 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09668 and P01040 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09668 and P01040 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09668 and P01040 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09668 and P01040 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09668 and P01040 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09668 and P01040 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09668 and P01040 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09668 and P01040 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09668 and P01040 |
Pubmed |
SVM Score :0.0 |
| NA |
|