| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| Sputum samples from 25 patients with bronchiectasis were assayed enzymatically for myeloperoxidase , neutrophil elastase and cathepsin B , and immunologically for cystatin A , cystatin B , cystatin C , cystatin S and kininogen . ^^^ These samples were also found to contain high levels of cathepsin B activity and cystatin A , but low levels of cystatin S and of the most effective cathepsin B inhibitor , cystatin C . ^^^ In contrast , sputum samples that were low in myeloperoxidase and neutrophil elastase activities had low levels of cathepsin B and cystatin A , but high cystatin C and S levels . ^^^ |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| The conjugated protein and purified cornified envelope showed an inhibitory effect against papain and cathepsin L , but cathepsin B and H were not inhibited by these P cystatin alpha conjugates . ^^^ |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| Possible functional consequences with regard to a role of cystatin A in the inhibition of the Alzheimer amyloid precursor protein ( APP ) clipping enzyme , cathepsin B , are discussed . . ^^^ |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| The bone pit formation induced by parathyroid hormone ( PTH ) was markedly suppressed by leupeptin , E 64 and cystatin A , while no inhibition was observed by CA 074 , a specific inhibitor of cathepsin B . ^^^ |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| Characterization by kinetic and equilibrium methods of the interactions of cystatin A Gly 4 mutants with papain , cathepsin B , and cathepsin L . ^^^ The latter finding indicates that the intact N terminal region serves as a guide directing cystatin A to the active site of cathepsin B , as has been proposed for cystatin C . ^^^ |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| Expression of cystatin A was used to inhibit cathepsin B , whereas Z Val Ala Asp fluoromethyl ketone ( Z VAD FMK ) was used to inhibit caspases . ^^^ Cystatin A expression prevented cathepsin B activation and apoptosis during treatment with glycochenodeoxycholate ( GCDC ) , a toxic bile salt . ^^^ Caspase N acetyl Asp Glu Val Asp 7 amino 4 methylcoumarin ( DEVD AMC ) hydrolytic activity increased in both wild type and cystatin A transfected cells treated with GCDC , demonstrating caspase activation despite inhibition of cathepsin B . ^^^ Our data demonstrate that 1 ) bile salt induced apoptosis can be inhibited by the cystatin A transgene and 2 ) caspase and cathepsin B activation are linked mechanistically with cathepsin B downstream of caspases . . ^^^ |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| It was slightly suppressed by N ( L 3 trans propylcarbamoyloxirane 2 carbonyl ) L isoleucyl L prol ine ( CA 074 ) , a selective inhibitor of cathepsin B , and by pepstatin , an inhibitor of cathepsin D , whereas it was markedly suppressed by a combination of cystatin alpha and either CA 074 or pepstatin . ^^^ |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| The weaker binding to cathepsin B was largely due to a considerably reduced association rate constant ( approximately 4 10 10 ( 4 ) M 1 . s 1 ) , consistent with the ' occluding loop ' of cathepsin B markedly restricting the access of cystatin A to the active site . ^^^ |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| The contribution of N terminal region residues of cystatin A ( stefin A ) to the affinity and kinetics of inhibition of papain , cathepsin B , and cathepsin L . ^^^ In contrast , the N terminal region is required also for an initial binding of cystatin A to cathepsin B , presumably by promoting the displacement of the occluding loop and allowing facile interaction of the rest of the inhibiting wedge with the active site cleft of the enzyme . . ^^^ |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| Contrary to caspase inhibitors , a panel of pharmacological cathepsin B inhibitors , the endogenous cathepsin inhibitor cystatin A as well as antisense mediated depletion of cathepsin B rescued WEHI S cells from apoptosis triggered by TNF or TNF related apoptosis inducing ligand . ^^^ |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| Replacement of the three N terminal residues preceding the conserved Gly of cystatin A by the corresponding 10 residue long segment of cystatin C increased the affinity of the inhibitor for the major lysosomal cysteine proteinase , cathepsin B , by approximately 15 fold . ^^^ The low dissociation rate constant for the binding of cystatin A to cathepsin B was retained by the chimeric inhibitor , which therefore had a higher affinity for this enzyme than any natural cystatin identified so far . ^^^ |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| In the present study , we investigated the expression and localization of the lysosomal cysteine proteinase cathepsin B and its natural inhibitors cystatin A , B and C in high ( MV 3 ) , intermediate ( SKmel 28 ) and low invasive ( SKmel 23 , WM 164 ) human melanoma cell lines grown on plastic or in contact with monomeric or fibrillar collagen type 1 . ^^^ |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| Chromosomal locations of human cathepsin B , stefin A and kininogen have been determined . ^^^ Using techniques of somatic cell hybrids and polymerase chain reaction , cathepsin B has been mapped to 8p22 , stefin A to 3cen q 21 , and kininogen to 3q26 qter . ^^^ |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| Stefin A , the physiological cathepsin B inhibitor , was less effective ; this might depend on its limited penetrability into living cells . ^^^ |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| In contrast , stefin A from sarcoma exhibited a reduced ability to inhibit papain , human liver cathepsins B , H and L and human and murine tumor cathepsin B . ^^^ The Ki for inhibition of liver cathepsin B by sarcoma stefin A was 10 fold higher than that for inhibition of liver cathepsin B by liver stefin A , reflecting a reduction in the rate constant for association and an increase in the rate constant for dissociation . ^^^ |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| The serum levels of lysosomal cathepsin B and L and Stefin A , an intracellular inhibitor of these proteolytic enzymes , were determined in patients with hepatocellular carcinoma ( HCC ) and / or liver cirrhosis ( LC ) and correlated with some clinical and biochemical parameters of these diseases . ^^^ These data suggest that cathepsin B and L and Stefin A may be potentially useful as additional biochemical parameters to monitor the therapeutic response of these diseases to clinical treatments and to identify patients with cirrhosis developing precancerous lesions . . ^^^ |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| Intraventricular infusion of stefin A , an inhibitor of cathepsin B , significantly reduces cerebral infarct volume in rats . . ^^^ |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| The contribution of the second binding loop of cystatin A to protease binding varies with the protease , being largest , approximately 45 % of the total binding energy , for inhibition of cathepsin B . . ^^^ |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| Moreover , IL 18 generation was reduced in THP 1 cells stably transformed either with cystatin A ( an endogenous cathepsin inhibitor ) or antisense cathepsin B cDNA . ^^^ |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| In addition , the concentrations of cathepsin B and L as well as of cathepsin inhibitors stefin A , stefin B , and cystatin C were measured by ELISA . ^^^ |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| The relationship of cathepsin B and stefin A mRNA localization identifies a potentially aggressive variant of human prostate cancer within a Gleason histologic score . ^^^ |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cathepsin B and its inhibitor stefin A in brain tumors . ^^^ Cysteine protease cathepsin B ( CatB ) and its endogenous inhibitor stefin A ( StA ) play an important role in tumor progression . ^^^ |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| Our purpose was to analyze the predictive relevance of DNA ploidy , S phase fraction ( SPF ) , and tissue levels of lysosomal proteinases cathepsin D ( CD ) , cathepsin B ( CB ) , cathepsin L ( CL ) , and urokinase type plasminogen activator ( uPA ) and that of the intracellular cysteine proteinase inhibitor stefin A on clinical outcome . ^^^ |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| Human stefin A is an inhibitor of lysosomal cysteine proteinases cathepsin B , H , L and S . ^^^ |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cathepsin B and L and stefin A and B levels as serum tumor markers in squamous cell carcinoma of the head and neck . ^^^ |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| Ratio of cathepsin B to stefin A identifies heterogeneity within Gleason histologic scores for human prostate cancer . ^^^ |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| In this study we investigated the levels of two lysosomal cysteine protease proteins cathepsin B ( CB ) and cathepsin L ( CL ) and the levels of three cysteine protease inhibitor proteins stefin A ( SFA ) , stefin B ( SFB ) and cystatin C ( CNC ) in squamous cell lung carcinoma ( SQCLC ) and matched lung parenchyma specimens and examined the inhibition of CB and cathepsin C ( CC ) activities by endogenous inhibitors in extracts from SQCLC , lung adenocarcinoma ( LAC ) and lung parenchyma specimens . ^^^ |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| Comparison of potential biological markers cathepsin B , cathepsin L , stefin A and stefin B with urokinase and plasminogen activator inhibitor 1 and clinicopathological data of breast carcinoma patients . ^^^ Enzyme linked immunosorbent assays ( ELISAs ) were used to measure cysteine cathepsin B ( CatB ) and cathepsin L ( CatL ) and their inhibitors , stefin A ( StA ) and stefin B ( StB ) , together with urokinase ( u PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) , in 150 cytosols of primary invasive breast carcinoma . ^^^ |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| Prediction of pelvic lymph node metastasis by the ratio of cathepsin B to stefin A in patients with prostate carcinoma . ^^^ |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| The aim of our study was to examine if concentration of stefin A in plasma of patients with diabetes type 2 is different than in healthy subjects , depends on vascular complications , body mass index , glycaemic control and whether exists the relationship between activities of cathepsin B ( CB ) as well as N acetyl beta D glucosaminidase ( NAG ) . ^^^ |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| Subsequently , we evaluated the effect of stefin A overexpression on cell growth , cathepsin B activity , cell motility and invasion , tumor growth , and metastasis . ^^^ Immunoanalysis was done to assess the expression of factor 8 and to support the localization of stefin A and cathepsin B . ^^^ RESULTS : Both transfection of stefin A and treatment with 10 micromol / L CA074Me significantly reduced cathepsin B activity and inhibited the Matrigel invasion . ^^^ |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01040 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|