| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.63263634 |
| It is argued that the N terminal cystatin C interaction with cathepsin B is physiologically important and hence that leucocyte elastase could have a function as a regulator of extracellular cysteine proteinase inhibitory activity at sites of inflammation . . 0.63263634^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| These data suggest that the arginyl residue of the tetrapeptidyl diazomethane , and also the corresponding arginyl residue in native cystatin C , interact with a S 4 substrate pocket subsite of both papain and cathepsin B . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cystatin C and cathepsin B in human colon carcinoma : expression by cell lines and matrix degradation . ^^^ Expression of the cysteine proteinase cathepsin B and its physiological inhibitor cystatin C was analyzed in vitro in 1 human fibrosarcoma and 4 human colon carcinoma cell lines . ^^^ Cystatin C antigen as well as cathepsin B activity were detected in the conditioned media of the 5 cell lines . ^^^ The corresponding cell extracts expressed high levels of cathepsin B activity , whereas only trace amounts of cystatin C antigen could be found . ^^^ Northern blot analysis revealed the presence in the 5 cell lines of a 0 . 8 kb cystatin C mRNA transcript and 2 cathepsin B transcripts of 2 . 3 and 4 . 3 kb . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Control of cathepsin B activity by the kininogens is incomplete and depends critically on the active concentration of cystatin C ( 70 nM in normal plasma ) , which is reduced in blood plasma of polytraumatized and septic patients and very low in epithelial lining fluid of the shock lung . . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Human sputum cathepsin B degrades proteoglycan , is inhibited by alpha 2 macroglobulin and is modulated by neutrophil elastase cleavage of cathepsin B precursor and cystatin C . ^^^ Cystatin C in purulent sputum was shown to be the N terminally truncated form generated by neutrophil elastase cleavage , and sputum cathepsin B was only weakly inhibited by recombinant cystatin C that had been cleaved by neutrophil elastase in vitro . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cystatin C , a potent inhibitor of cysteine proteases such as papain and cathepsin B , was examined for its effect on human coronaviruses OC 43 and 229e . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cystatin C and cathepsin B production by alveolar macrophages from smokers and nonsmokers . ^^^ The capacity of alveolar macrophages ( AM ) obtained from smokers and nonsmokers to secrete cathepsin B and its inhibitor cystatin C was examined because of the concept that an imbalance in the production of proteolytic enzymes and / or their inhibitors could be responsible for the lung damage seen in smokers . ^^^ Quantitation of immunoprecipitates on Western blots showed that the amount of total cystatin C secreted into the culture medium by AM of smokers was significantly greater than the amount secreted by cells obtained from nonsmokers , whereas the difference between the amount of cathepsin B secreted by the AM of smokers and that from nonsmokers did not appear significant . ^^^ Cathepsin B was not detected in Western blots at its reported molecular weights but was identified at the exact area occupied by the higher molecular weight cystatin C , i . e . , at bands corresponding to 69 Kd , 63 Kd , and 57 . 3 Kd . ^^^ Therefore , it is clear that in culture media of AM , cystatin C and cathepsin B are present as proteinase antiproteinase complexes . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Sputum samples from 25 patients with bronchiectasis were assayed enzymatically for myeloperoxidase , neutrophil elastase and cathepsin B , and immunologically for cystatin A , cystatin B , cystatin C , cystatin S and kininogen . ^^^ These samples were also found to contain high levels of cathepsin B activity and cystatin A , but low levels of cystatin S and of the most effective cathepsin B inhibitor , cystatin C . ^^^ In contrast , sputum samples that were low in myeloperoxidase and neutrophil elastase activities had low levels of cathepsin B and cystatin A , but high cystatin C and S levels . ^^^ Although this may be due in part to reduced amounts of cathepsin B inhibitors , particularly cystatin C , theoretical considerations suggest that factors other than the gross level of inhibitors must be involved in the control of cathepsin B activity . . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Synthesis and secretion of procathepsin B and cystatin C by human bronchial epithelial cells in vitro : modulation of cathepsin B activity by neutrophil elastase . ^^^ The medium also contained the cathepsin B inhibitor , cystatin C , but cystatins A , B , S , SN , SA , and kininogen were not detected . ^^^ Cleavage both of cystatin C and procathepsin B by neutrophil elastase is essential for the generation of cathepsin B activity in the medium . . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| The interaction between cystatin C variants , in which the evolutionarily conserved Gly 11 residue was substituted by Ala , Glu or Trp , and the cysteine proteinases , papain , ficin , actinidin and cathepsin B , was characterized . ^^^ Comparison with the affinities of wild type cystatin C lacking the N terminal region indicated that even this small structural change affects the conformation of this region sufficiently to largely abolish its interaction with the weakly binding proteinases , actinidin and cathepsin B . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Interactions from the side chains of residues in the N terminal binding region and Trp 106 are jointly responsible for the major part of cystatin C affinity for cathepsin L and are also of considerable importance for cathepsin B and H affinity . ^^^ The contribution of the Arg 8 side chain is minor , but significant for cystatin C interaction with cathepsin B . ^^^ The Leu 9 side chain confers selectivity to the inhibition of the target peptidases ; it contributes to cathepsin B and L affinity by factors of 200 and 50 , respectively , to cathepsin S binding by a factor of 5 only , and results in a 10 fold decreased affinity between cystatin C and cathepsin H . . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Thus , the cellular cathepsin B kinetics for the synthetic substrates Z Arg Arg 4M beta NA and Z Val Lys Lys Arg 4M beta NA , its pH dependence and inhibition by E 64 , stefins A and B , and cystatin C could be determined . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| The results demonstrate that 1 ) cystatin D has a characteristic inhibition profile since it does not inhibit cathepsin B ( Ki > 1 microM ) , and when compared to cystatin C and all other known cystatins it is a much poorer inhibitor of cathepsin L ( mean Ki 25 nM ) but binds cathepsin H and S relatively tightly ( mean Ki values of 8 . 5 and 0 . 24 nM , respectively ) ; and 2 ) the inhibitory activities of the two cystatin D variants are not significantly different , demonstrating that the presence of an extra cysteine residue in the cystatin D molecule affects neither the stability nor the functional activity of the inhibitor , thus explaining the widespread distribution of the Cys 26 cystatin D encoding allele in the population . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cathepsin B and cystatin C were determined by ELISA test in 47 patients with rheumatoid arthritis , seronegative spondylarthritis , osteoarthritis , undifferentiated arthritis and gout . ^^^ The elevation of cathepsin B was accompanied by an increased amount of cystatin C . . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cathepsin B was efficiently inhibited by cystatin C at pH 7 . 4 , whereas the inhibition by stefin B and high molecular weight kininogen was only moderate . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Parallel physicochemical and functional investigations of L68Q cystatin C and wild type cystatin C revealed that both proteins effectively inhibit the cysteine proteinase cathepsin B ( equilibrium constants for dissociation , 0 . 4 and 0 . 5 nM , respectively ) but differ considerably in their tendency to dimerize and form aggregates . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Evidence for the interaction of valine 10 in cystatin C with the S 2 subsite of cathepsin B . ^^^ The interactions between wild type or mutant recombinant forms of human cystatin C and rat cathepsin B were characterized by measuring progress curves for substrate hydrolysis in the presence of inhibitor . ^^^ With cystatin C that has Val 10 replaced by an Arg residue ( Val10Arg cystatin C ) , the inhibition constant , K ( 1 ) , increased 31 fold if the isosteric substitution Glu 245 to Gln was made in cathepsin B . ^^^ The observed effects on K ( 1 ) indicate that the additional positive charge introduced in Val10Arg cystatin C is interacting with the negative charge on Glu 245 in cathepsin B when these two proteins form a complex ; the cystatin variant is thus binding in a substratelike manner with this region of the enzyme . ^^^ Indirectly , these results suggest that when native cystatin C and cathepsin B form a complex , Val 10 in the inhibitor interacts with the S 2 subsite of the enzyme . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| The association rate constants for the binding of recombinant human cystatin C to papain , ficin , actinidin and recombinant rat cathepsin B were 1 . 1 10 10 ( 7 ) , 7 . 0 10 10 ( 6 ) , 2 . 4 10 10 ( 6 ) and 1 . 4 10 10 ( 6 ) M 1 . s 1 , whereas the corresponding dissociation rate constants were 1 . 3 10 10 ( 7 ) , 9 . 2 10 10 ( 6 ) , 4 . 6 10 10 ( 2 ) and 3 . 5 10 10 ( 4 ) s 1 . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Latency of cathepsin B secreted by human colon carcinoma cells is not linked to secretion of cystatin C and is relieved by neutrophil elastase . ^^^ The cell lines also secrete a physiological inhibitor of cathepsin B , cystatin C . ^^^ Ammonium chloride significantly increased latent cathepsin B levels without affecting the constitutive secretion of cystatin C . ^^^ Phorbol 12 myristate 13 acetate induced a 4 to 5 fold increase in secreted latent cathepsin B , but did not alter significantly the accumulation of cystatin C in media . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Their inhibitory properties were compared with those of wild type recombinant cystatin C by determination of the equilibrium constants for dissociation ( Ki ) of their complexes with the cysteine endopeptidases papain and human cathepsin B and with the cysteine exopeptidase dipeptidyl peptidase 1 . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| This role is partly in contrast to that of the N terminal region , which increases the affinity of cystatin C for cathepsin B by increasing the association rate constant . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Determination of equilibrium constants for dissociation ( Ki ) for their complexes with the target proteinase , cathepsin B , produced values not largely different from that for human cystatin C ( Ki 0 . 07 0 . 13 nM ) . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cystatin C rapidly ( kass = 5 . 5 10 10 ( 7 ) M 1s 1 ) and tightly inhibits papain ( Ki = 0 . 02 nM ) , whereas its interaction with bovine cathepsin B is substantially weaker ( Ki = 4 . 4 nM ) . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Studies at the protein level , allowed through E . coli expression of wildtype and L68Q mutated cystatin C genes , revealed that both protein variants effectively inhibit the cysteine proteinase cathepsin B ( equilibrium constants for dissociation : 0 . 4 and 0 . 3 nM , respectively ) , but differ considerably in their tendency to dimerize and form aggregates . ^^^ The three dimensional structure of normal cystatin C , crystallized in a complex with cathepsin B , was elucidated by 10 ray analysis and subsequent refinement of the structure to 3 . 0 A resolution . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| To clarify the significance of the constituents of canine senile plaques ( SPs ) or cerebrovascular amyloid deposits , paraffin and cryostat sections of canine brains were examined by immunohistochemistry using antibodies against cathepsin B ( CB ) , cathepsin D ( CD ) , cystatin C ( CC ) , alpha 1 antichymotrypsin ( ACT ) , heat shock protein 70 ( HSP 70 ) , ubiquitin ( Ubq ) , and apolipoprotein E ( Apo E ) . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| The mature recombinant protein processed by insect cells started at amino acid 4 ( cystatin C numbering ) , and displayed reversible inhibition of papain and cathepsin B ( Ki values of 0 . 39 and 32 nM , respectively ) , in competition with substrate . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| The expression and function of cystatin C and cathepsin B and cathepsin L during mouse embryo implantation and placentation . ^^^ With the regression of the decidua beginning on day 9 . 5 , a coordinated upregulation of both cathepsin B and cystatin C was observed implying a role for controlled cathepsin expression during apoptosis . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Two step mechanism of inhibition of cathepsin B by cystatin C due to displacement of the proteinase occluding loop . ^^^ Stopped flow kinetics showed that the inhibition of the lysosomal cysteine proteinase , cathepsin B , by its endogenous inhibitor , cystatin C , occurs by a two step mechanism , in which an initial , weak interaction is followed by a conformational change . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| The affinity of the modified inhibitor for cathepsin B was 20 fold lower ( Ki 5 nM ) than that of full length cystatin C . ^^^ The cathepsin B inhibitory activity of cystatin C incubated with gingival crevicular fluid was rapidly abolished after Val 10 bond cleavage by elastase from exudate neutrophils , but cleavage at the gingipain specific Arg 8 bond was also demonstrated . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| The latter finding indicates that the intact N terminal region serves as a guide directing cystatin A to the active site of cathepsin B , as has been proposed for cystatin C . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : To study the expression of the cysteine proteinases cathepsin B and L and their most potent inhibitor cystatin C in the synovial membrane of patients with rheumatoid arthritis ( RA ) and osteoarthritis ( OA ) on both the messenger RNA ( mRNA ) level and the protein level . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| In addition , the concentrations of cathepsin B and L as well as of cathepsin inhibitors stefin A , stefin B , and cystatin C were measured by ELISA . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Expression of cathepsin B and cystatin C in human colorectal cancer . ^^^ In addition , the expression of cystatin C , one of several cathepsin B inhibitors , was compared with that of cathepsin B in the same samples to reveal one of the regulation mechanisms of cathepsin B . ^^^ Conversely , cystatin C mRNA levels were similar in all samples , and tended to show an inverse correlation with the cathepsin B levels . ^^^ In conclusion , cathepsin B expression by human colorectal cancers and surrounding noncancerous cell components may contribute to both local invasion at the early stage and remote metastasis without influence of cystatin C . . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| The Ki value for the interaction was 0 . 20 nM , i . e . cystatin C had an affinity for legumain similar to that for the papain like family C 1 cysteine peptidase , cathepsin B . ^^^ However , cystatin C variants with alterations in the N terminal region and the `` second hairpin loop ' ' that rendered the cystatin inactive against cathepsin B , still inhibited legumain with Ki values 0 . 2 0 . 3 nM . ^^^ This was corroborated by analysis of a cystatin C variant with substitution of the Asn 39 residue in this loop ( N39K cystatin C ) ; this variant showed a slight reduction in affinity for cathepsin B ( Ki 1 . 5 nM ) but > > 5 , 000 fold lower affinity for legumain ( Ki > > 1 , 000 nM ) than wild type cystatin C . . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Incubation of cystatin C with papain , cathepsin B or cathepsin H led to no changes in the cystatin C molecule . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| The mature recombinant protein processed by insect cells had an N terminal segment 7 residues longer than that of cystatin C and displayed reversible inhibition of papain and cathepsin L ( Ki = 1 . 1 and 0 . 31 nM , respectively ) , but not cathepsin B . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| The concentrations of immunologically detected cathepsin L and cystatin C , but not cathepsin B , were significantly increased in BAL fluid from the smokers with emphysema compared with those without emphysema , although the activity of cathepsin L , measured using a synthetic substrate and cathepsin L , released from cultured alveolar macrophages at 24 h , did not show any significant difference between the two groups . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| In this study we investigated the levels of two lysosomal cysteine protease proteins cathepsin B ( CB ) and cathepsin L ( CL ) and the levels of three cysteine protease inhibitor proteins stefin A ( SFA ) , stefin B ( SFB ) and cystatin C ( CNC ) in squamous cell lung carcinoma ( SQCLC ) and matched lung parenchyma specimens and examined the inhibition of CB and cathepsin C ( CC ) activities by endogenous inhibitors in extracts from SQCLC , lung adenocarcinoma ( LAC ) and lung parenchyma specimens . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cathepsin B and its endogenous inhibitor cystatin C in rheumatoid arthritis synovium . ^^^ OBJECTIVE : To compare the expression of cathepsin B and its endogenous inhibitor cystatin C in synovial tissue of patients with rheumatoid arthritis ( RA ) and to determine the cell type expressing cystatin C . ^^^ METHODS : The expression of cathepsin B and cystatin C was studied by immunohistochemistry in synovial tissue of 10 patients with RA and compared to healthy controls . ^^^ Applying double labeling methods , the expression of cathepsin B was compared to that of cystatin C . ^^^ RESULTS : Both cystatin C and cathepsin B were strongly expressed in synoviocytes of patients with RA . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Synovial giant cells in rheumatoid arthritis : expression of cystatin C , but not of cathepsin B . ^^^ This study was designed to investigate the expression of the matrix degrading proteinase cathepsin B and its endogenous inhibitor cystatin C in rheumatoid arthritis ( RA ) with special regard to multinucleated synovial giant cells ( SGC ) . ^^^ SGC strongly expressed cystatin C and CD 68 , but were negative for cathepsin B . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Distribution , activity and concentration of cathepsin B and cystatin C in the wall of aortic aneurysm . ^^^ Intensity of immunohistochemical reaction to cathepsin B is stronger and to cystatin C weaker in the wall of aortic aneurysm than in normal aorta . ^^^ Localization of cathepsin B and cystatin C in the aneurysm wall is different from that in the control aorta , but activity and concentration of cystatin C in the aneurysmal wall is lower than in normal aorta . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Modulation of invasive properties of murine squamous carcinoma cells by heterologous expression of cathepsin B and cystatin C . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Expression of cathepsin B and cystatin C in the human adenohypophysis and in pituitary adenomas . ^^^ The localization of cathepsin B , a potential promoter of prolactin ( PRL ) release via extra renal renin angiotensin system in the adenohypophysis , and its inhibitor cystatin C in the human adenohypophysis and in pituitary adenomas were examined using single and dual immunohistochemical staining . ^^^ PRL producing adenomas strongly expressed cathepsin B , but only weakly expressed cystatin C , a pattern of staining contrary to the expression in normal PRL producing cells . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cathepsin B / cystatin C complex levels in sera from patients with lung and colorectal cancer . ^^^ A sandwich type ELISA has been developed for quantification of the complex between the cysteine proteinase cathepsin B ( CB ) and its reversible tight binding inhibitor cystatin C ( CC ) in normal and pathological sera . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Immunostaining of cystatin C within neurons showed a punctate distribution , which co localized with the endosomal / lysosomal proteinase , cathepsin B . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cystatin C and cathepsin B in CSF from patients with inflammatory neurologic diseases . ^^^ Cystatin C , a major cysteine protease inhibitor in CSF , is tightly bound to cathepsin B and H . ^^^ OBJECTIVE : To determine if cystatin C is involved in the disease process of IND , the authors measured the cystatin C concentration by ELISA method and cathepsin B and H activities in the CSF of patients with acute IND . ^^^ METHODS : Cystatin C concentration and cathepsin B and H activities were measured in CSF samples taken from patients during the acute phase of their disease . ^^^ Decreased levels of cystatin C may be related to the high levels of cathepsin B activity seen in the CSF of patients with GBS , CIDP , and MS . . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Applying double labeling techniques , the coexpression of cathepsin B and its major endogenous inhibitor cystatin C was additionally studied . ^^^ Coexpression of cathepsin B and cystatin C occurred only in a few MC . ^^^ Because previous studies showed that osteoclasts express high levels of CD 68 , cathepsin K , and cystatin C but not of cathepsin B , our study contributes to the view that GC of GCTTS and osteoclasts are closely associated . . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Colocalization of cathepsin B and its endogenous inhibitor cystatin C occurred in the majority of MGC . ^^^ Mononuclear cells ( MC ) were positive for cathepsin B , cystatin C , and CD 68 , but only faintly for cathepsin K . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Expression of cathepsin B and cystatin C in human breast cancer . ^^^ In this study , we investigated the expression of cathepsin B and cystatin C , the strongest inhibitor of cathepsin B , and measured the relative amounts of each in human breast cancer tissues . ^^^ Cystatin C expression relative to cathepsin B expression was found to be decreased . ^^^ This report documents the first simultaneous investigation of cathepsin B and cystatin C in breast cancer tissues . . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| One of them , Z Arg Leu Val Agly Ile Val OMe ( compound 17 ) , which contains in its sequence the amino acid residues from the N terminal binding segment as well as the hydrophobic residues from the first binding loop of human cystatin C , proved to be a highly potent and selective inhibitor of cathepsin B . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| We observed progressive reductions in levels of the protease inhibitor cystatin C , an inhibitor of cathepsin B with corresponding increases in the malignancy of glioma cell lines , implying an inverse correlation between cystatin C and tumor grade . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| In contrast to mouse recombinant cystatin C , recombinant CRES did not inhibit the cysteine proteases papain and cathepsin B , suggesting that it probably does not function as a typical cystatin . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cathepsin B and H activities and cystatin C concentrations in cerebrospinal fluid from patients with leptomeningeal metastasis . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| The activities ' of the lysosomal cysteine proteinases cathepsin B and L are regulated by their endogenous inhibitors , stefins A and B , and cystatin C , and their imbalance may be associated with increased invasiveness and development of the malignant cell phenotype . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Matrix metalloproteinase ( MMP ) 1 , 2 , 10 , 13 and 14 , tissue inhibitor of MMP ( TIMP ) 1 , 2 and 3 , cathepsin B and D , and cystatin C were expressed under basal conditions , of which MMP 10 and cystatin C expression have not been described before in endothelial cells . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Replacement of the three N terminal residues preceding the conserved Gly of cystatin A by the corresponding 10 residue long segment of cystatin C increased the affinity of the inhibitor for the major lysosomal cysteine proteinase , cathepsin B , by approximately 15 fold . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| The role of cathepsin B and cystatin C in the mechanisms of invasion by ovarian cancer . ^^^ OBJECTIVE : The aim of this study was to investigate the contribution of cathepsin B and cystatin C to the mechanisms of invasion by ovarian cancer . ^^^ MATERIALS AND METHODS : Using surgical materials from patients with ovarian cancer , immunohistochemistry , sodium dodecyl sulfate polyacrylamide gel electrophoresis ( SDS PAGE ) and Western blotting analysis were performed using antibodies against cathepsin B or cystatin C . ^^^ Serum levels of cathepsin B and cystatin C in patients with benign and malignant ovarian lesions were determined by enzyme linked immunosorbent assay ( ELISA ) . ^^^ An invasion assay using an ovarian cancer cell line was performed by addition of cystatin C or specific inhibitors of cathepsin B . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cystatin C is a strong endogenous inhibitor of lysosomal cysteine proteases , such as cathepsin B , L , H and S , that are involved in various biological processes such as degradation of cellular proteins and regulation of enzymes , as well as in pathological processes . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| The mRNA and protein levels of cathepsin B , L , and cystatin C and M in breast cancer specimens were determined utilizing laser capture microdissection / RT PCR , Western blotting , and immunohistochemical methods . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Staining of cathepsin B , D , H , L , stefin B and cystatin C was observed in the cytoplasm of epithelial cells in decidua . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Also , hypoxia led to down regulation of the inhibitors cystatin C and B , resulting in increased residual activity of cathepsin B . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cystatin C is the strongest inhibitor of cathepsin B . ^^^ Cystatin C and cathepsin B expressions were evaluated by immunohistochemical methods and by semiquantitative real time polymerase chain reaction analysis for the corresponding messenger RNA . ^^^ Tumors with low cystatin C protein expression and high cathepsin B protein expression were significantly more likely to be of high grade , and this pattern was significantly correlated with high Ki 67 LI and tumor recurrence . ^^^ Multivariate analysis demonstrated high tumor grade , high Ki 67 labeling index , high cathepsin B expression , and low cystatin C expression correlated significantly with shorter disease free survival . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Neuronal cell death induced by cystatin C in vivo and in cultured human CNS neurons is inhibited with cathepsin B . ^^^ We further confirmed that cystatin C neurotoxicity was inhibited by simultaneous coapplication of cathepsin B , a cysteine protease . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| In addition , alterations in expression of the lysosomal cysteine and aspartic proteases , cathepsin B ( CB ) and cathepsin D ( CD ) and the endogenous cysteine protease inhibitor cystatin C were observed utilizing immunocytochemical analysis at 24 , 48 , and 72 h following 6 OHDA exposure . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Although total cystatin C levels in the MS patients was not different compared with controls , the patients with the highest 12 . 5 / 13 . 4 peak ratios also had the greatest cathepsin B inhibitory activity . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Expression of SerpinB 3 and cystatin C , relative to the target , protease cathepsin B , was independently predictive of response ( odds ratio , 17 . 8 ; 95 % CI , 2 . 0 to 162 . 4 ; P = . 01 ) , with an accuracy of 72 % . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cystatin C ( CST 3 ) is a secreted inhibitor of lysosomal cysteine proteases cathepsins B ( CTSB ) and CTSL , which are abundant in the ovine endometrium and conceptus . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| Localization of the mRNA species for cystatin C and cathepsin B , as well as , localization of protein species for cystatin C , cathepsins B and L were performed to evaluate the tissue distribution of these species . ^^^ |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P01034 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|