| Interacting proteins: Q2Y0W8 and P00918 |
Pubmed |
SVM Score :0.0 |
| We investigated whether NBC 3 , like some Cl ( ) / HCO ( 3 ) ( ) exchange proteins , could bind CAII and whether PKA could regulate NBC 3 activity through modulation of CAII binding . ^^^ CAII bound the COOH terminal domain of NBC 3 ( NBC3Ct ) with K ( d ) = 101 nM ; the interaction was stronger at acid pH . ^^^ Cotransfection of HEK 293 cells with NBC 3 and CAII recruited CAII to the plasma membrane . ^^^ Mutagenesis of consensus CAII binding sites revealed that the D 1135 D1136 region of NBC 3 is essential for CAII / NBC3 interaction and for optimal function , because the NBC 3 D1135N / D1136N retained only 29 + / 22 % of wild type activity . ^^^ Coexpression of the functionally dominant negative CAII mutant V143Y with NBC 3 or addition of 100 microM 8 bromoadenosine to NBC 3 transfected cells reduced intracellular pH ( pH ( 1 ) ) recovery rate by 31 + / 3 , or 38 + / 7 % , respectively , relative to untreated NBC 3 transfected cells . ^^^ |
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| Interacting proteins: Q2Y0W8 and P00918 |
Pubmed |
SVM Score :0.0 |
| This resistance to inhibition by the physiological anions bicarbonate and chloride suggests an evolutionary adaptation of CA 13 to the presence of high concentrations of such anions ( e . g . , in the reproductive tract of both female and male ) , and the possible participation of this isozyme ( similarly to CA 2 , CA 4 and CA 5 ) in metabolons with proteins involved in the anion exchange and transport , such as the anion exchangers ( AE 1 3 ) or the sodium bicarbonate co transporter ( NBC 1 and NBC 3 ) proteins , which remain to be identified . . ^^^ |
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| Interacting proteins: Q2Y0W8 and P00918 |
Pubmed |
SVM Score :0.0 |
| Functional and physical interactions also occur between CAII and Na+ / HCO3 ( ) co transporter isoforms NBC 1 and NBC 3 . ^^^ |
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| Interacting proteins: Q2Y0W8 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
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